LOCUS       DASWPU010000001         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_85589,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000001 DASWPU010000000
VERSION     DASWPU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85589"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VGC27_00005"
     CDS             <1..514
                     /locus_tag="VGC27_00005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004406.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY0280992.1"
                     /translation="ALAVFELALEEKSLEAVERDFGALKTMVLESADLARFVRAPVFS
                     RSEQAKGMTAILGKMDAAPLSKRFVLLLAAKRRLYLLLDVIRDFDSLLARQRGEIDAE
                     IVSARPLAEGETAELKRVLKSKFGREPQLAKRVDPTLLGGLVVKVGSRMIDSSLRTKL
                     IGIRAAMRGS"
     gene            625..2160
                     /gene="atpA"
                     /locus_tag="VGC27_00010"
     CDS             625..2160
                     /gene="atpA"
                     /locus_tag="VGC27_00010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY0280993.1"
                     /translation="MMNIQPAEISAILKREIQNFGAEAEVSEVGQVLSVGDGIARVYG
                     LDNVQAGEMVEFPGGIKGMALNLETDNVGVVIFGSDTTIKEGETVKRTGAIVDVPVGK
                     GLLGRVVDPLGEPIDGKGPLTNVAERRRVDVKAPGIIPRRKVSEPMQTGIKAIDALIP
                     IGRGQRELIIGDRQTGKTAIIVDTIINQKGINAGSDETKKLYCIYVAIGQKLSTVAQI
                     VKTLEDAGAMEYTTVVAATASEPAPLQFLAPFAGCAMGEWFRDNGMHAVIFYDDLSKQ
                     AVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNADNGSGSLTALPVIETQAN
                     DVSAYIPTNVISITDGQIFLETELFYQGIRPAVNVGISVSRVGSSAQVKAMKQIAGSI
                     KLDLAQYREIAAFAQFGSDLDASTQRLLNRGARLTELLKQPQFTPMPVEEQVVSLYAG
                     MRGYLDKLTVSNVGRFEADLLRLVRAKHKVVLDEIRIGGMLTPEIDAKLKAILDEFAK
                     AFD"
     gene            2160..>2370
                     /locus_tag="VGC27_00015"
     CDS             2160..>2370
                     /locus_tag="VGC27_00015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY0280994.1"
                     /translation="MASLKSLRNRIASVKATRKITKAQQMVAASKFRRALDAAIAARP
                     YAEKMGDVIASLAGGLKDNPGAPKLL"
BASE COUNT          460 a          777 c          734 g          399 t
ORIGIN      
        1 cgcccttgcc gttttcgagc tagcgcttga agaaaaatcc ctcgaggcgg tggagcgcga
       61 ttttggcgct ttgaagacga tggttttgga aagcgccgac ctcgcccgct tcgtccgcgc
      121 acccgtgttc agccgcagcg aacaggccaa gggcatgacg gcgatcctcg gcaagatgga
      181 cgcagcgccg ctgagcaagc gtttcgtgct gctgctcgcc gccaagcgcc ggctgtattt
      241 gctgctcgat gtcatacggg acttcgattc cctgctggcc cgccagcgcg gcgagatcga
      301 tgccgaaatc gtctccgcgc gcccgctggc cgaaggcgaa accgccgaac tcaagcgcgt
      361 gctcaagtcc aagttcggcc gcgaaccgca gcttgcaaag cgggtcgatc ccacgcttct
      421 cggcggcctg gtcgtgaaag tgggttcgcg catgatcgat tcctcgttgc gcacgaagct
      481 gatcggcata cgcgccgcga tgagagggag ttgaatccta ttgccgccct cacccgtcga
      541 ccagctcagg tgagggacta gccaaacccc tcaggctgag cttgtcgaag cttgagggcg
      601 ccagaaccga gtaagggaac gagactgatg aatatccaac cggccgaaat ctccgccatc
      661 ctcaagcgcg agatccagaa tttcggcgcc gaagcggaag tgagcgaagt cggccaagtg
      721 ctgagcgtcg gcgacggcat cgcccgcgtc tacggcctcg ataatgtcca ggccggcgag
      781 atggtcgagt tcccgggcgg catcaagggc atggccctga acctggaaac cgacaatgtc
      841 ggcgtcgtga tcttcggttc cgacaccacc atcaaggaag gcgaaacggt caagcgcacc
      901 ggcgccatcg tcgatgtgcc cgtgggcaag ggcttgctcg gccgcgtcgt cgatccgctg
      961 ggcgaaccga tcgacggcaa gggcccgctc accaacgtcg ccgaacgccg ccgcgtcgat
     1021 gtgaaggcgc caggcatcat tccgcgccgc aaggtgtccg agccgatgca gaccggcatc
     1081 aaggccatcg acgcgctgat cccgatcggc cgcggccagc gcgagctgat catcggcgac
     1141 cgccagaccg gcaagacggc catcatcgtc gatacgatca tcaaccagaa agggatcaac
     1201 gccggcagcg acgagacaaa gaagctctat tgcatctacg tcgccatcgg ccagaagctt
     1261 tccaccgtgg cgcagatcgt caagacgctg gaagacgccg gcgcgatgga atacaccacg
     1321 gtcgtcgccg ccacggcttc cgagcctgcg ccgttgcagt tcctggcgcc gttcgccggt
     1381 tgcgccatgg gcgaatggtt ccgcgacaac ggcatgcacg ccgtgatctt ctacgacgat
     1441 ctctccaagc aggccgtggc ctatcgccag atgtcgctgc tgctgcgccg tccgccggga
     1501 cgcgaggcct atccgggtga cgtgttctat ctgcattccc gcctgctgga gcgcgccgcc
     1561 aagatgaacg ccgacaacgg ttcgggctcg ctgacggcat tgccggtcat tgaaacccag
     1621 gccaacgacg tctcggccta catccccacc aacgtgatct cgatcaccga cggccagata
     1681 ttcctggaaa ccgagctgtt ctatcagggc atccgcccgg cggtgaacgt cggcatctcg
     1741 gtcagccgcg tgggatcgag cgcgcaagtc aaggcgatga agcagatcgc cggctcgatc
     1801 aagctggacc tggcgcaata ccgcgagatc gcggccttcg cccagttcgg ttcggacctt
     1861 gacgcctcga cgcagcgcct gctcaatcgc ggcgcgcgcc tcaccgaact cctgaagcag
     1921 ccgcagttca cgccgatgcc ggtcgaggaa caggtggttt ccctgtacgc cggcatgcgt
     1981 ggctatctcg acaagctcac cgtctccaat gtcggccgtt ttgaggccga tctgctgcgc
     2041 ctggtgcgcg ccaagcacaa ggtcgtgctc gacgagatcc gcatcggcgg catgctgacg
     2101 cccgagatcg atgccaagct gaaggccatt ctggacgaat ttgcgaaagc cttcgactga
     2161 tggcgagcct gaagtccctc cgcaaccgca tcgcctcggt caaggcgacg cgcaagatca
     2221 ccaaggccca gcagatggtg gcggcctcga agttccgccg cgccctggat gccgcgatcg
     2281 ccgcgcgtcc ctatgccgaa aagatgggcg acgtgatcgc cagtttggcc ggcggcctga
     2341 aagacaatcc gggcgcgccg aagctgcttg
//
LOCUS       DASWPU010000002         4730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_55047,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000002 DASWPU010000000
VERSION     DASWPU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55047"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..1522
                     /gene="coaBC"
                     /locus_tag="VGC27_00020"
     CDS             323..1522
                     /gene="coaBC"
                     /locus_tag="VGC27_00020"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY0280995.1"
                     /translation="MRARSVLLIIGGGIAAYKSLDLIRRLKERGISTRVILTKAGCEF
                     VTPLTVSALTGGKVYSDLFNLTDEAEMGHIQLSRSAELVVVAPATADLMAKLANGLAN
                     DLASAALLATDKQVLMAPAMNVRMWNHPATRRNLKTLKNDGVLFVGPNDGEMACGEYG
                     PGRMAEPNEIAAAIESALAAGAAGPLAGLKALVTAGPTQEPLDPVRFIANRSSGKQGY
                     AIAEALAKAGAETILVSGPVEIAPPPQVKLLRVETAREMLAACESALPVDVAVCAAAV
                     ADWRPEITPGNKIKKSEGPPAIKLTANPDILSTLARAPRRPALLIGFAAETENVLANA
                     TEKRAKKGCDWIVANDVGGGVMGGDRNKVHLITADGTESWPEMEKSEVGTRLAARIAG
                     ALKGKAT"
     gene            1519..1986
                     /gene="dut"
                     /locus_tag="VGC27_00025"
     CDS             1519..1986
                     /gene="dut"
                     /locus_tag="VGC27_00025"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY0280996.1"
                     /translation="MTKIEIMRLPHAADLPLPSYATGGSAGLDLLAAVDAEIELKPGA
                     RVLVPCGIAIALPAGFEAQVRPRSGLALHHGVTLLNAPGTIDSDYRGEVKATLINHGD
                     IAFRITRGMKIAQMIVARHETAELHEVGELPASERGAGGFGSTGLHIAAERTA"
     gene            1990..2439
                     /locus_tag="VGC27_00030"
     CDS             1990..2439
                     /locus_tag="VGC27_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0280997.1"
                     /translation="MLKLSRKTLLALEAVIDIAFNARPEPVQAKEITARQGVPQRYLE
                     QVMQQLVRAGILRGVRGPRGGYRLAKERRRISVGDIVRAAESIEEESDESAPRSELGS
                     QIVSPFIQTLQGDIMAKLDAITIEDLCQRARAAGVETGDSITADFTI"
     gene            2450..3409
                     /gene="cysK"
                     /locus_tag="VGC27_00035"
     CDS             2450..3409
                     /gene="cysK"
                     /locus_tag="VGC27_00035"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY0280998.1"
                     /translation="MTDRKPGRGRIYNSITETIGDTPLVRLARLPGQSGVKADILLKL
                     EFFNPISSVKDRIGVAMVEALEQQGLISPGKNTLVEPTSGNTGIALAFVAAAKGYKLI
                     LVMPETMSIERRKMLTLLGAQVVLTEGPKGMKGAIAKAQEIVATTPGAVVPQQFENPA
                     NPDIHRRTTAEEIWNDTDGKVDVVISGVGTGGTITGVGQVLKAKKPGVKMIALEPEDS
                     PVLSGGQPGPHKIQGIGAGFVPAVLDRKVIDEVITISNETAFETARKLARLEGIPVGI
                     SSGAAVAAALEVGARSEMAGKTIVAIIPSFAERYLSTALFEGL"
     gene            complement(3448..4386)
                     /locus_tag="VGC27_00040"
     CDS             complement(3448..4386)
                     /locus_tag="VGC27_00040"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY0280999.1"
                     /translation="MRPWNIAHRGGARSRPENTLAAFAHAVANGCDGAELDVQLSRDG
                     EVVVFHDYRLKPEICRDEKGRWLVAPTPRLKDLSLAELKRFDIGRADPASVYAREHGG
                     VVWQDGERIPTLDEVVAAAKRAPAPFRLFVELKTSFFDRDASALPEYLAERTVAVVKA
                     HDYLEQTVFVGFDWPGLLHAKKIAPDVQCWFSTLPQSWFRDGVPPPEDGPPAEPALQV
                     LRYWAREGISPWAGGHDAVRHGGSIVAAVYAAGGDGWFPAWTDATEKAVRQAHGFGLK
                     VGAWTVDEPAVMRRLAALGLDAICTDRPDLMKADSE"
     gene            complement(4413..>4730)
                     /locus_tag="VGC27_00045"
     CDS             complement(4413..>4730)
                     /locus_tag="VGC27_00045"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0281000.1"
                     /translation="LSRVIVHEPIVGGSDLKVRVAGTAIRRRFGRDVTGETMSNLFPA
                     DSFPARFKSVLTAIETGAPQFAECQLSTSSFEILRSELVILPVLAPDRVSKWAMTFAL
                     FFN"
BASE COUNT          928 a         1521 c         1484 g          797 t
ORIGIN      
        1 tcgtccagaa gaaccgataa tgcgaaccag aagctggcgc gctccttctc accttccgcc
       61 gagaacgagg caaaggcttg ttgtgcataa tctcgcgcaa tcaagccatg ttcggcagcc
      121 aattcggctg ccaggaagca cagatcgccg gtggaaatac gcatcgcggc cacgttcttg
      181 acgacgcggc catattgtca gaatagggtg actgtctggt taaggacgtt tagctgccgg
      241 aattgcttga aactacattt acttttcgtt atttttaata aataccttgt ttttatcggt
      301 atttcccatt agggaggctc tcatgcgcgc ccgaagcgtc ctgctcatca tcggcggcgg
      361 cattgccgcc tataaatcgc tggacttgat ccgccggctc aaggagcgcg gcatctccac
      421 gcgcgtgatc ctgacgaagg ccggctgcga attcgtcacc ccgctcacag tgtccgccct
      481 gacaggcggc aaggtctatt cggacctgtt caacctgacc gacgaagccg aaatgggcca
      541 tatccagctc tcgcgcagcg ccgagcttgt cgtcgtggcg ccggcaaccg ccgacctgat
      601 ggccaagctg gcgaacgggc tcgcgaacga cctggcctcc gccgcgctgc tggcgaccga
      661 caaacaagtg ctgatggcgc cggcgatgaa cgtgcgcatg tggaaccatc cggccacccg
      721 ccgcaacctc aagaccttga agaacgacgg cgtgctcttc gtcgggccga acgacggcga
      781 aatggcgtgc ggcgaatacg ggccgggccg tatggccgag cccaacgaaa tcgcggcggc
      841 gatcgagagc gcattggcgg cgggcgccgc cggaccactt gcgggcctca aggcgctagt
      901 aacggcagga ccgacgcaag agccgctcga tcccgtgcgc ttcatcgcca accgttcctc
      961 gggcaagcag ggctacgcga ttgccgaggc cctcgcgaag gcgggggccg agactattct
     1021 cgtctccggg ccggtcgaaa tcgcgccgcc gccgcaggtg aaactcctgc gcgtggaaac
     1081 cgcgcgcgag atgctggcgg catgcgagtc cgccttgccg gtggacgtgg cggtttgcgc
     1141 ggcggctgtc gccgactggc ggccggaaat cacccctggc aacaagatca agaaatccga
     1201 aggtcctccc gcgatcaagc tgacagcaaa ccctgacatt ctttcgacgc tcgcgcgcgc
     1261 tccgcgccgc cccgccctgt tgatcggctt cgcggcggaa acggagaacg tcctcgccaa
     1321 cgccaccgag aagcgcgcca aaaaaggctg cgactggatc gttgccaacg atgtcggcgg
     1381 cggcgtcatg ggcggcgacc gcaacaaggt ccatcttatc actgcggacg gcactgaaag
     1441 ctggccggaa atggagaaga gcgaggtggg cacccggctt gccgcacgca tcgccggcgc
     1501 cttgaagggc aaagcaacat gacgaagatc gagatcatgc gcctgccgca cgcggccgat
     1561 ctgcccctgc cttcctacgc gaccggcggc tcggccggac tggacttgct cgccgccgtc
     1621 gatgcggaga tcgaactgaa gccgggtgcg cgggttctgg tgccgtgcgg catcgccatt
     1681 gccctgcccg cgggcttcga ggcgcaggtg cgtccgcgct ccggcctggc gttgcaccac
     1741 ggcgttaccc tgttgaacgc gccgggcacg atcgacagcg attaccgcgg cgaggtgaaa
     1801 gccacgctca tcaatcacgg cgatatcgcc ttccgcatca cgcgcggcat gaagatcgcc
     1861 cagatgatcg tcgccaggca cgaaaccgcc gagcttcacg aggtcggcga attgcccgcg
     1921 agcgaacgtg gtgccggcgg cttcggttcg acgggacttc atatcgcagc cgaaaggacg
     1981 gcctgaccca tgctcaagct ttcccgaaag acgttgctgg ctttggaagc ggtgatcgat
     2041 atcgccttca acgcgcgccc agagccggtc caggccaagg aaatcaccgc gcgccagggt
     2101 gttccgcagc gctatctgga gcaggtgatg cagcagttgg tgcgcgccgg aattctcaga
     2161 ggcgtgcgcg gaccgcgcgg cggctatcgc ctcgccaagg agcgcagacg gatatccgtg
     2221 ggcgatatcg tgcgcgcggc cgaaagcatc gaagaagaaa gcgacgaaag cgcgccgcgc
     2281 tccgaactcg gctctcagat cgtgtcgccc tttatccaga cgttgcaggg cgacatcatg
     2341 gcgaagctgg atgccatcac catcgaggat ttgtgtcagc gtgcccgtgc cgccggtgtc
     2401 gaaaccggcg acagtatcac cgccgatttc acgatttagg agaacgacaa tgaccgatcg
     2461 caagcccggc cgcggccgta tttataattc catcaccgag actatcggcg acacgcccct
     2521 ggtgcgcctg gcgcgcctgc ccgggcagtc cggcgtcaaa gcggacatcc tgctcaagct
     2581 cgaattcttc aatcccatct ccagcgtcaa ggaccgcatc ggcgtggcga tggtggaggc
     2641 gctggaacag caaggcctga tttcgccggg caagaacacc ctggtggagc cgactagcgg
     2701 caataccggc atcgcgctgg ccttcgtggc cgccgccaag ggctacaagc tcattctggt
     2761 gatgcccgaa accatgtcga tcgagcgccg caagatgctc acgctcctgg gcgcacaggt
     2821 ggtgctgacc gaaggcccca agggcatgaa gggcgccatc gccaaggcgc aggagatcgt
     2881 ggccacgacg ccgggcgcgg tcgtcccgca gcaattcgag aaccccgcca atcccgatat
     2941 ccatcgccgc accacggccg aggaaatctg gaacgatacc gacggcaagg tcgacgtcgt
     3001 gatttccggc gtcggcacgg gtggcacaat caccggcgtt ggtcaggtgt tgaaggcgaa
     3061 gaagcccggc gtgaagatga tcgcgctcga acccgaggat tcgccggtac tgtccggcgg
     3121 ccagcccggc ccgcacaaga ttcagggcat cggcgcgggc ttcgtgccgg cagtcctcga
     3181 ccgtaaggtg atcgacgagg tcatcaccat ctccaacgag accgcctttg aaactgcacg
     3241 caagctggcg cggctggaag gcattcccgt cggcatctct tccggcgcgg cggttgcggc
     3301 ggcgctcgaa gtgggcgcgc ggtcggagat ggcgggcaag accatcgtgg cgatcatccc
     3361 gtcgttcgcg gagcgctatt tatcgacggc gctgttcgaa ggattataac ggcgaatggc
     3421 gaagtagcga agagccacct attcgcttta ttcgctatcc gctttcatca aatccggcct
     3481 gtccgtgcag attgcatcga ggccgagcgc ggcgagacgg cgcatgacgg ccggctcgtc
     3541 gaccgtccaa gcgccgacct tgaggccgaa gccgtgcgcc tggcgtactg ctttttccgt
     3601 cgcatcggtc caggcgggaa accagccgtc gccgccggcc gcatatactg ccgcgacgat
     3661 cgagccgcca tgcctgaccg cgtcgtggcc gcctgcccag ggcgaaatcc cttcgcgcgc
     3721 ccaatagcgc agcacctgca gcgccggttc ggcaggcggt ccgtcctcgg gcggcggcac
     3781 cccgtcgcga aaccagcttt gcggcaaagt cgagaaccag cattgtacgt cgggcgcaat
     3841 cttcttcgca tgcagcagcc ccggccaatc aaagccgacg aagaccgttt gctccagata
     3901 atcgtgcgcc ttcaccaccg caaccgttcg ttcggcgaga tattccggca gcgccgaggc
     3961 atcccggtcg aaaaaagacg ttttcaattc cacaaacagg cggaacggcg caggcgctct
     4021 ctttgctgcc gccaccactt catccagggt cgggatgcgc tcgccgtcct gccagacgac
     4081 gccgccgtgt tcgcgcgcat agacgctagc agggtcggcg cgtccgatat cgaagcgctt
     4141 gagttcggca agcgataaat ccttcaagcg cggcgtcggc gcgacaagcc accgcccttt
     4201 ttcgtcgcgg cagatttcag gcttcaggcg ataatcgtgg aacacgacta cctcgccgtc
     4261 gcgcgacaac tgcacatcga gttcggcccc gtcgcagccg ttcgccacgg catgggcgaa
     4321 ggccgccagc gtgttttcgg ggcgcgaccg cgcgccgccc cgatgagcga tgttccaggg
     4381 ccgcatgggc ggaccctata ccagttcgag agtcaattga aaaacagagc gaaggtcatg
     4441 gcccatttgc tgacgcgatc aggcgccaga accggcaata tcaccaactc ggaacgtaat
     4501 atctcgaacg aactggtgct gagttggcac tcggcaaatt gcggagcgcc ggtttctatg
     4561 gccgtcagaa cggatttgaa gcgagcggga aacgagtccg ccggaaacag gttcgacatg
     4621 gtttcgccgg tgacgtcacg gccgaagcgc cgccggatcg cggtaccggc gacccgcacc
     4681 ttgagatcgc tgccgcccac gatcggttcg tgaacgatga cgcggctcag
//
LOCUS       DASWPU010000003         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_67273,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000003 DASWPU010000000
VERSION     DASWPU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67273"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VGC27_00050"
     CDS             <1..1096
                     /locus_tag="VGC27_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HEY0281001.1"
                     /translation="YPASLFGSVAATSGRYADLATIESIPPPNLRIQLGANLSLREYG
                     SLAASWIEIRRREQDTAQLASASYMLSFGDRWYMGATGFYDCANRNWKAEVFLSLSFN
                     GDFIGHAAVRSGTGTHEEEIGLTKSINPDGGFGYRLSASTGDNNRGLAEATWIGRHGR
                     LDGGISAINGQVATRMLASGAIVAIDGSLYATQVPNGAVALVRTGEEGVHIYRENRDV
                     AIADKDGEALLTGLVPYTKNRVSIEPHDYRLSTIVETTEKIVAPPRMSGVVVDLAPKS
                     RHPVIITLHLADGSPPPLGSRVVRNDGGEPLVVGRDGEIFIADFLHAVKGTVEYGEGP
                     CSFAVAPLASSPPDSILRIGPVLCSKDVTP"
     gene            1093..1599
                     /locus_tag="VGC27_00055"
     CDS             1093..1599
                     /locus_tag="VGC27_00055"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HEY0281002.1"
                     /translation="MRMKTPGRVWCLSGSAALFLASTITPALAAECGTGINPILVVAT
                     GAAFGNYSPSSGTPATTNGTITLTCTVVLLSTLPSFTVSLSASTSGQINPRQMSFGAA
                     RLNYNLFTTASYTTIWGNGTAPTVTQSYASGDNLVLKTFTVYGRIPAGQVVAVGLYTD
                     SLTVTITY"
     gene            complement(1703..>3421)
                     /locus_tag="VGC27_00060"
     CDS             complement(1703..>3421)
                     /locus_tag="VGC27_00060"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY0281003.1"
                     /translation="LARDVRGFAVKIYTKEGNWDLVGNNIPVFFIQDAIKFPDLIHSV
                     KPEPDRDFPQAQSAHDNFWDFVSLSPESLHMIMWVMSDRGIPRSFRFMEGFGVHTFRL
                     LGAKDRSTFVKFHWKPKLGLQSVMWNEAVKINGADPDFHRRDLWDAINSGDFPEWEFG
                     VQLFDDKFAEKFDFDVLDPTKLIPEEILPVRIVGRLVLNRVVENFFAETEQVAFCTQN
                     VPPGIDFSNDPLLQGRNFSYLDTQVKRLGGPNFTHIPINAPKCPFAHFQQDGHMTMRN
                     PTGRVNYEPNSWGAEGGPRENPDAGFRSFAAEESGPKTRLRPESFADHYSQARQFYIS
                     QTPVEQKHIGDAIVFELSKVERPDIRSRVVSHLIHIDADLAKTVANGLGLDLPKPAQA
                     ARPVNTKLPPSAALSMLKKPIDSFAGRKLGILATDGADAEIFHALVAAVGKEKAVYDV
                     VAPKIGGVTLSNGTKVVAKQKIDGGPSVLFDAVAILASKDGAKLLAGDSTAKDFVNDA
                     FAHCKFIGYSPEAMALFEKAGLAKDLDDACIALGARADAGAFVKACRALRHWEREPKI
                     DLDAKA"
BASE COUNT          676 a         1041 c          996 g          708 t
ORIGIN      
        1 ctatccggcg agcctcttcg gctcggtggc ggccacatcg ggtcgctatg cggatctggc
       61 caccattgaa agcattccgc cacccaatct gcgaattcag ctcggcgcca atctcagtct
      121 tcgggaatat ggttctctgg cggcgtcatg gattgaaatc cgccgccggg aacaagacac
      181 cgcccagctt gcctcggcgt cctatatgct gtcctttggc gaccggtggt atatgggcgc
      241 gacaggcttc tacgattgtg ccaaccgcaa ttggaaggcc gaagtgtttc tcagcctttc
      301 ctttaacggc gacttcatcg gccacgcggc ggtccgttcc ggaacaggca cccatgaaga
      361 ggaaatcgga cttaccaaat cgatcaatcc cgacggcgga ttcggatacc ggctatcggc
      421 gtccaccggc gataacaata gaggactggc ggaagcgacg tggatcgggc ggcatggcag
      481 gctggacggg gggatctccg cgataaacgg acaagttgcg acccgtatgc tggcatccgg
      541 cgcgatcgtt gcaatagacg gttcgctcta cgcgacacaa gtgcccaacg gcgccgtcgc
      601 gctggtgcgt acgggtgaag aaggcgtaca catctatcgc gaaaaccgcg atgtggcgat
      661 cgcggataag gacggcgagg ctctgctcac gggcctggta ccctacacca aaaatcgcgt
      721 ctccatcgag ccgcacgact accggctttc cacgatcgtc gagacgacgg agaaaatcgt
      781 cgcgccgccc cgcatgagcg gggtggttgt tgacctggcg cccaaatccc gtcatccggt
      841 tatcatcacc ttgcatctgg cggacggcag ccccccgccg ctcggttcgc gggtcgtgcg
      901 gaacgacggc ggagaaccgc ttgtcgtggg gcgggacggc gaaatattta tcgctgattt
      961 tctgcatgcc gtgaagggaa cggtcgaata tggcgaaggc ccatgcagtt tcgccgtggc
     1021 gcctctcgcc tcatcgccgc cggattccat tctgcggatc ggacccgttc tctgctcgaa
     1081 ggatgtcacg ccatgaggat gaaaacgcca ggacgggttt ggtgcctttc gggaagcgcc
     1141 gcactgtttc tggcttcgac gataacgcct gcattggcgg cagaatgcgg caccgggatt
     1201 aatccgatcc tggtcgtggc gaccggggcc gcctttggca actattcccc cagttctggt
     1261 acgcctgcaa cgacgaatgg caccatcaca cttacctgca cggtcgtgct cctctccacc
     1321 ctgcccagct tcacagtcag cctctccgcc agtacaagtg gtcagattaa tccccggcaa
     1381 atgagcttcg gtgcggcccg gctgaactac aatcttttta ccaccgcctc gtataccacg
     1441 atttggggca acggtacggc cccgaccgtg acgcaatcct acgcatccgg cgacaactta
     1501 gttctgaaga cgttcacggt gtatggccgc atccctgcag gccaggttgt ggcggtcggc
     1561 ctctatacag acagccttac ggtgacgatc acttattgat atcgcatcca atttcttttc
     1621 cggcacagcc gactctcctc ggttcgcggg tacaacacac gttgcttcat caacaccttg
     1681 aggtttcggt tggttcttcc tttcacgcct ttgcgtcgag atcgatcttc ggttcgcgct
     1741 cccagtggcg cagggcgcgg caagccttga cgaaagcgcc ggcatcggcc ctcgcgccca
     1801 gcgcgatgca ggcgtcatcc agatccttcg caaggcccgc cttctcaaac agcgccatag
     1861 cctccggcga gtagccgatg aacttgcaat gagcgaaagc gtcgttgacg aaatccttcg
     1921 cggtcgaatc tccagccaag agtttcgcac cgtccttcga tgcgaggatc gcgaccgcgt
     1981 cgaacagcac cgacgggccg ccatcgatct tctgtttggc gacgaccttg gttccgttcg
     2041 acagggtaac gccgccgatc ttcggcgcga cgacatcata gaccgccttc tccttgccga
     2101 cagccgcgac aagcgcatgg aatatctccg catccgcgcc gtcggtcgcc aggataccga
     2161 gcttgcggcc agcgaaactg tcgatgggct tcttcaacat gctcaatgct gcagacggcg
     2221 gcagtttggt attgaccggc cgcgcggcct gtgccggctt gggcagatcg aggccaaggc
     2281 catttgccac ggttttcgcc aggtctgcgt cgatatggat gaggtgtgac accacgcgcg
     2341 aacggatgtc cggccgctcg accttgctca attcgaacac gatggcatcg ccgatatgct
     2401 tttgctcgac cggtgtctga ctgatgtaga actggcgcgc ctggctgtaa tggtcggcga
     2461 aactctccgg ccgcaatcgc gtttttgggc cgctctcttc cgcggcgaag ctgcgaaagc
     2521 cggcatccgg gttctcgcgc gggccacctt ccgcgcccca actgttgggc tcgtaattaa
     2581 cgcggccggt gggattgcgc atcgtcatgt ggccgtcttg ttggaaatgg gcaaacggac
     2641 atttgggcgc gttgatcggg atatgcgtga agttggggcc gcccaaccgc ttcacctgcg
     2701 tgtcgagata ggagaagtta cggccctgaa gcagcggatc gttgctgaag tcgatccccg
     2761 gcgggacgtt ctgggtgcag aaggcaacct gctcggtttc ggcgaagaaa ttctccacca
     2821 cgcggttcag caccagccgc ccgacgatcc tgaccggcag aatctcttcc ggaataagct
     2881 tggtcggatc gagcacgtcg aagtcgaatt tctccgcgaa cttatcgtcg aaaagctgca
     2941 caccaaactc ccattccggg aaatcgccgc tgttgatggc atcccacaga tcgcggcggt
     3001 ggaaatccgg atcggcgccg ttgatcttca ccgcttcgtt ccacatcacc gattgcaggc
     3061 cgagcttcgg cttccagtgg aatttgacga aggtcgaacg gtctttcgcg ccgagcaagc
     3121 gaaaggtatg gacgccgaac ccttccatga aacgaaaaga gcgcggaatc ccccggtccg
     3181 acatcaccca catgatcatg tgcaggctct cgggcgaaag cgacacaaaa tcccagaaat
     3241 tgtcgtgcgc cgattgtgcc tgcggaaagt cgcgatcggg ctctggtttc acggaatgga
     3301 tgagatcggg aaacttgatc gcgtcctgga tgaagaagac gggaatgttg ttgccaacca
     3361 gatcccagtt tccttccttg gtataaatct tgactgcgaa accgcgcacg tcgcgcgcaa
     3421 g
//
LOCUS       DASWPU010000004         4745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_116183,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000004 DASWPU010000000
VERSION     DASWPU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116183"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..965
                     /locus_tag="VGC27_00065"
     CDS             147..965
                     /locus_tag="VGC27_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0281004.1"
                     /translation="MTEPLYLSRGGERVAYVRRGGAGPGIVWLGGFRSDMEGTKAQAV
                     DAWAARSGRACLRFDYFGHGRSSGDFLRGTISRWKDDALAVLDALTAGPQILVGSSMG
                     GWIAMLAAKARHERVAGLVLIAPASDFTEALMWERMPQAARGAIERDGVWRRESDDAE
                     DCYPISRALIEDGRMNLILDEQHKLPYPVRILQGMADPDVPWPHALKLAGAIDGEVTV
                     TLVKNGDHRLSRPADLKLLERTLDALLEDVDKRNLYPPLEGGANGQRPFGEESC"
     gene            959..1711
                     /locus_tag="VGC27_00070"
     CDS             959..1711
                     /locus_tag="VGC27_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281005.1"
                     /translation="MLKRILPVMGILIFLPFPAAAAGGPTDYSQCVALVQKNPALAEE
                     NARAWQNEGGGAAAIHCSALALTALKRYAEAARKFDALARDKTIGNNLDRAELFDQAG
                     NAWLLAGMANDAVRSFSAALDHSQDNVDFLADRARARAMLKDWAGADADLGAALLGDQ
                     NRVDLLVLRASARWALNRRTDAATDILRALSLYPDYPAALVERGRMKYEAGDKNGARA
                     DWKKAAALGEGETAEAARRYLAEMGEESKTGK"
     gene            complement(1694..2824)
                     /gene="queG"
                     /locus_tag="VGC27_00075"
     CDS             complement(1694..2824)
                     /gene="queG"
                     /locus_tag="VGC27_00075"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY0281006.1"
                     /translation="MPNSISDIREALRARAAAEGFDVVRFARAAATQEAAQRLGAFLE
                     QGRHGTMDWLAKNAERRADPAQLWPEAKSVVVLGANYGPDHNPLDDLKTRIAGAISVY
                     ARGDDYHDALKKRLKRLAGFMVTAFGGDAKVFIDTAPVLEKPLAQEAGLGWQGKHTNL
                     VSREFGSWLFLGSVFTTLEIEADAPERDHCGACHACLDICPTAAFPAPYRLDARRCIS
                     YLTIEHKGHIPREFRQAIGNRIYGCDDCLAVCPWNKFARVSHEAQFRAREALMRPLLA
                     ELARLDDAAFRALFRASPIKRIGRDRFVRNVLIAIGNSGEAALAAEAERLLDDASALV
                     RAMAVWALSKLADARRVAALSSKYRPQETDGDVQEEWLLARL"
     gene            complement(2805..3449)
                     /locus_tag="VGC27_00080"
     CDS             complement(2805..3449)
                     /locus_tag="VGC27_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0281007.1"
                     /translation="MRRLVHLVFQPASRLARLMLGEKRLVVDPQAAEDATAHLPVFTD
                     LDGTRCEGLWAIVDHLEGAYPEVPLVPEEAAARGETMRWLDWSMTVLGERVTKKIVFE
                     KANPRFTGALTRSTPDMNVIRRGREALHEILPKIGETVERRGNLAGRICTIADLALAA
                     HLSALDYFGEVPWDATPAVKEWYVRMKSRPSFRSLLADRVPGQPPVTLYAELDF"
     gene            complement(3488..4243)
                     /gene="mtgA"
                     /locus_tag="VGC27_00085"
     CDS             complement(3488..4243)
                     /gene="mtgA"
                     /locus_tag="VGC27_00085"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY0281008.1"
                     /translation="MGMLSEIRNRLAAFARWIWWGGGERTPWLRRIAAAVFFFLAPAP
                     VLYLLIFRFVPVPVTPQILLDAVTFQPVKYSWRAYDDISPYLARAVIGSEDQDFCNHH
                     GFAWKNIDKALKQHERHPKKRLRGASTISQQVARTLFLLPARSWVRKGAEAYLTVLLE
                     AFWPKKRILTAYLNLVDWGHGNYGAEAAAEVYFGVSADRLTKAQAARLATVLPNPDKW
                     RAARPGRYVASRTGIVEDRAWEVTRDGLDWCVK"
     gene            complement(4281..>4745)
                     /locus_tag="VGC27_00090"
     CDS             complement(4281..>4745)
                     /locus_tag="VGC27_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0281009.1"
                     /translation="FTLIEKAGADASHTVGAGKKFFARPRPLVGNDAPICIERGDIAK
                     SYSYPSGHTTQGWAYALILAELVPDRTNEIAARGRAYGESRVVCGVHWASDVAAGRVA
                     ASALVAALHGSRAFRADMNKARAEIASFRKTAPPPVQKICAVSNAAAAKQPW"
BASE COUNT          818 a         1541 c         1598 g          788 t
ORIGIN      
        1 gcaataagcc accgtgccga attcgccata tttcccgctc ccgttcgttc tccggcgaag
       61 cttagcagat tttattgcgg cgcacagatt gttcgcgggg tatggaatat gcgatgacat
      121 ctcatggaat atttccgccg acagccatga ccgaacctct ttatctgagc cgcggcgggg
      181 agcgtgtcgc ctatgttagg cgcggcggcg ccgggccggg catcgtctgg ctcggcggat
      241 tccgctccga catggaaggt acgaaagcgc aggcggtcga tgcctgggcc gcgcggagcg
      301 gccgcgcctg tctgcggttc gactatttcg gccacggccg ttcgtcgggt gattttctgc
      361 gcggcacgat ctcgcgctgg aaggacgatg ccttggccgt actcgatgcg ctgacggcgg
      421 ggccgcaaat cctggtgggc tcgagcatgg gcggctggat cgcgatgctg gcggcgaagg
      481 cgcggcatga gcgcgtggcc ggactggtgc tgatcgcgcc cgcgtcggat ttcaccgaag
      541 ccctgatgtg ggagcggatg ccgcaagcgg cgagaggcgc gatcgagcgg gacggcgtgt
      601 ggcggcgcga gtccgatgat gccgaggact gctatccgat cagccgcgcg ctgatcgaag
      661 acggacgcat gaacctcatc ctcgacgaac aacataagct gccgtatccg gtgcgtattc
      721 tacagggcat ggccgacccc gacgtgcctt ggccgcacgc gctgaagctc gccggagcga
      781 ttgacggcga ggtgaccgtg accctggtga aaaacggcga ccaccggctg tcgcggcccg
      841 cggatctcaa actgctggaa cgcacgctcg acgccttgct ggaggacgta gacaagagaa
      901 acctttaccc tccccttgag ggaggggcga acggccaaag gccgttcggg gaggaatcat
      961 gttgaagcgc atcttgcccg tgatgggcat tctcatcttt cttccgtttc cggccgccgc
     1021 agccggcggc cccacggatt attcgcaatg cgtcgcgctg gtgcaaaaaa atccggcgct
     1081 cgccgaggaa aacgcccgcg cctggcagaa cgaaggcggc ggggccgccg cgattcattg
     1141 ttcggcgctg gcgctcaccg cgctcaagcg ttacgccgag gcggcgcgca agttcgatgc
     1201 gctggcccgc gacaaaacga tcggcaataa tttggatcgc gcggagctgt tcgatcaagc
     1261 gggcaacgcc tggctgctcg ccggcatggc gaacgatgcc gtgcggtctt tttccgccgc
     1321 gcttgaccac agccaggaca atgtcgattt cctcgccgac cgcgcacggg cgcgcgccat
     1381 gctgaaggat tgggccggcg ccgatgccga tctcggcgcg gcgctgttgg gggaccagaa
     1441 ccgcgtcgat ctcttggtgt tgcgcgccag cgcgcgctgg gcgttgaacc gcaggacgga
     1501 cgccgccacc gatattctgc gcgcgctctc gctgtatccc gattacccgg cggcgctggt
     1561 cgagcgcggc cggatgaaat acgaggcggg cgacaagaac ggcgcgcgcg ccgattggaa
     1621 gaaagcggcg gccttgggcg agggagagac ggccgaagcc gcaaggcgct atctggcgga
     1681 gatgggcgag gagtcaaaga cgggcaagta gccactcttc ttgcacgtcc ccatccgttt
     1741 cctgcggccg atacttggag gaaagcgccg caacccgccg tgcatccgcg agtttcgaca
     1801 gtgcccacac cgccatggcg cgcaccaggg ccgaggcatc gtccagcagc cgttcggctt
     1861 ccgccgcaag cgccgcctcg ccggagttgc cgatggcgat cagcacgttc cgcacgaagc
     1921 ggtcgcggcc gatgcgcttg atcggcgagg cgcggaacag tgcgcggaac gcggcatcgt
     1981 ccagccgcgc cagctcggcg agcagcgggc gcatgagcgc ttctcgcgcg cgaaactgcg
     2041 cctcatgcga aactcgcgcg aatttgttcc acgggcatac cgccaggcaa tcgtcgcagc
     2101 cataaatgcg gttgccgatg gcctggcgga actcgcgcgg gatatgaccc ttatgctcga
     2161 tggtgaggta ggaaatgcag cgccgcgcat cgagccggta gggcgcggga aaggcggccg
     2221 tcgggcaaat gtccaggcaa gcatgacatg cgccgcaatg atcgcgctcc ggcgcatcgg
     2281 cctcgatctc aagtgtcgtg aacaccgagc cgaggaagag ccaggagccg aactcgcggc
     2341 tgaccaaatt cgtatgcttg ccttgccagc cgaggccggc ttcctgcgcc agcggctttt
     2401 cgagcaccgg cgcggtatcg atgaagacct tcgcgtcgcc gccgaacgcc gtcaccatga
     2461 acccggccag ccgcttgagc cgcttcttca gcgcgtcgtg gtaatcgtcg ccgcgcgcat
     2521 agacggaaat cgcgcccgct atgcgggttt tcagatcgtc cagcggattg tgatcggggc
     2581 cgtaattcgc gcccaacacc accacgctct tcgcttcggg ccagagctgg gccggatcgg
     2641 cgcgtcgctc cgcgttcttg gccagccagt ccatcgtgcc gtgccggcct tgttccagga
     2701 acgcgcccag cctttgggcg gcctcctgcg tggccgcggc gcgggcaaag cggaccacgt
     2761 cgaagccctc cgcggcggcg cgggcgcgca gggcttctcg gatgtcagaa atcgagttcg
     2821 gcatagagcg tcaccggcgg ctggccgggc acgcggtcgg cgagcagcga gcggaacgag
     2881 ggccgcgact tcatccgcac ataccattcc ttcacggcgg gggtggcgtc ccacggcact
     2941 tcgccgaaat agtccagcgc cgacagatgc gccgccagcg ccagatcggc gatggtgcag
     3001 atccgccccg ccagattgcc gcgccgttcc accgtctcgc cgatcttggg cagtatttca
     3061 tgcaatgcct cgcgcccgcg gcggatgacg ttcatgtcgg gggtggagcg ggtgagcgcg
     3121 ccggtgaagc gcggattggc cttctcgaag acgattttct tggtcacgcg ctcgcccagc
     3181 acggtcatcg accagtcgag ccagcgcatc gtctcgccgc gcgccgccgc ctcttccgga
     3241 accagcggaa cctccggata agcgccttcc agatggtcca cgatggccca caacccctcg
     3301 cagcgcgtgc cgtccaagtc ggtgaagacg ggcaaatggg cggttgcgtc ctccgcggcc
     3361 tgcggatcga cgacgaggcg cttttcaccc agcatcaggc gcgcaaggcg cgaggcgggc
     3421 tggaagacga ggtggacgag gcggcgcatg cgggcaatct agtgaaactg cgggcgggct
     3481 cctagcctta cttcacgcac caatccagcc cgtcgcgcgt cacttcccag gcgcggtcct
     3541 ccacgatgcc cgtgcggctt gccacgtagc ggccggggcg cgcggcgcgc catttgtcgg
     3601 ggtttggcag aacggtcgcc agccgcgcgg cctgcgcctt ggtcaggcga tccgcggaga
     3661 ctccgaaata aacctcggcc gccgcctcgg cgccgtaatt gccgtgcccc caatcgacca
     3721 ggttcagata ggcggtaagg atgcgcttct tcggccagaa ggcttccagc agcacggtca
     3781 gataagcctc cgcgcctttg cgcacccagc ttcgcgccgg cagcaggaac agggtgcgcg
     3841 cgacctgctg gctgatcgtg ctggcgccgc gcaggcgctt cttgggatgg cgctcgtgct
     3901 gcttcagcgc cttgtcgata ttcttccagg cgaagccgtg atggttgcag aagtcctggt
     3961 cctcggaacc gatgaccgcg cgcgccagat agggcgaaat gtcgtcatag gcgcgccagg
     4021 agtatttcac cggctggaaa gtgacggcat cgagcaggat ctgcggcgtg acgggcacgg
     4081 gcacgaagcg gaagatcagc agatacagaa ccggcgccgg cgccaggaag aagaagaccg
     4141 ccgccgcgat gcgccgcagc cacggcgtac gctccccgcc gccccaccag atccagcggg
     4201 cgaaggcggc gagccggttg cgaatctcgg acagcatccc catgggcgca agcttatcaa
     4261 accgcggcga tcccggcacc ctaccaaggt tgctttgccg cagccgcgtt cgaaacggcg
     4321 caaatctttt gaacgggggg cggtgccgtt ttgcggaagg aggcgatttc ggcgcgcgcc
     4381 ttgttcatgt ccgcgcggaa tgccctgctt ccgtgcagcg cggcgaccag cgcgcttgcg
     4441 gccacgcgcc cggccgcgac atcgctggcc caatgcacgc cgcagacgac gcggctttcg
     4501 ccataagccc gcccccgcgc cgcgatttcg ttcgtccggt cgggcacgag ttcggccagg
     4561 atcaaggcat aggcccagcc ctgagttgtg tggccggacg gataggaata gctcttggcg
     4621 atatcgccgc gttcgatgca gatcggcgca tcgttgccga ccagcgggcg tggacgggcg
     4681 aagaacttct tacccgctcc gacggtatga ctggcatccg cccccgcttt ttcgatcaac
     4741 gtaaa
//
LOCUS       DASWPU010000005         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_79528,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000005 DASWPU010000000
VERSION     DASWPU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79528"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VGC27_00095"
     CDS             <1..456
                     /locus_tag="VGC27_00095"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEY0281010.1"
                     /translation="HAMEQGGIARQPLLVPEVVNLDPAEVERLASRAGELAELGLVVE
                     AFGPDAVMVREVPAMLGQMDLKGLMRDLADDIAETGNALSLKERLEEVAGTLACHTSV
                     RAGRRLTAEEMNALLREMESTPHSGQCNHGRPTYVELKLSDIEKLFGRR"
     gene            complement(557..928)
                     /locus_tag="VGC27_00100"
     CDS             complement(557..928)
                     /locus_tag="VGC27_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0281011.1"
                     /translation="MRTILLGIVLATLLSVPLNASQQVETEQPVICSNENHWNNALPD
                     IITLTITADGKIMWNGNTVSPEIFSDYLSRTGKSNPQPVINIFPRPNVTYATLAPILH
                     KIQKHGIRDIWFGKVGKWIED"
     gene            complement(946..1254)
                     /locus_tag="VGC27_00105"
     CDS             complement(946..1254)
                     /locus_tag="VGC27_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281012.1"
                     /translation="MSKRTKLGLAIEEGLREAVAWKRGGIELPTRTIEPMAAKRVKAI
                     RKSVAKSPREFERRFGVPARTIEGWEQGKKVDVAARVLLTVIEKEPEAVERALEDFEQ
                     "
     gene            complement(1251..1580)
                     /locus_tag="VGC27_00110"
     CDS             complement(1251..1580)
                     /locus_tag="VGC27_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281013.1"
                     /translation="MPFVKIVRTHRYVKDIKKLKASAADVDEMERMIAADPLAGALIQ
                     GLKGVRKARFRIGNRGKSGGGRAIYYVMMADDTVLMITAYAKAEKEDLSPDDRKAILR
                     MLEELNR"
     gene            complement(1817..>2265)
                     /locus_tag="VGC27_00115"
     CDS             complement(1817..>2265)
                     /locus_tag="VGC27_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281014.1"
                     /translation="AEALNAAQWGFAVVFDCQSQQSSRNIATAVGNVKLTAYSCGTRD
                     GGFFVAVADYPARSITAKSIDAAYTGAVTGAASNIKGKIRRVTPYKLGKITGRDALID
                     VKENNQTAHLRVFYVRNRQFQITFIGPKGRENSKASLAFLNSFALK"
BASE COUNT          465 a          671 c          628 g          501 t
ORIGIN      
        1 catgcgatgg aacagggcgg catcgcccgc cagcccttgc tggtgcccga agtggtgaat
       61 ctcgatcccg ccgaggtcga acgcctggcg tcacgcgcgg gtgaactcgc cgaattgggc
      121 ctggtggtcg aagccttcgg ccccgacgcc gtgatggtgc gcgaagtgcc cgccatgctg
      181 ggccagatgg acctcaaggg cctgatgcgc gatctcgccg acgacattgc cgaaaccggc
      241 aacgccctgt cgctcaagga acgcctcgaa gaggtcgccg gaacgctggc ctgccacacc
      301 tcggtgcgcg ccgggcgcag gctcaccgcc gaggagatga acgcgctgtt gcgcgaaatg
      361 gaatccaccc cccattcggg ccaatgcaac cacggaaggc cgacgtatgt ggagctgaag
      421 ctctccgaca tcgaaaagct atttggacgg agatgatttg ttctgccctc cctttgaggc
      481 aggaccgatc aagccgaagc gcgtggcgcg aaggcagatc aaggagaggt gaggttcatg
      541 tttttttaac gtgagcttag tcctcaatcc acttcccaac cttgccgaac caaatgtcac
      601 gaattccgtg tttctgaatc ttatgcagga ttggagccaa ggtggcatag gttacatttg
      661 ggcgaggaaa gatattgatc acaggctgcg gattactttt accagttcgc gaaaggtaat
      721 ctgagaatat ctccgggctc actgtgttgc cgttccacat tatctttcca tctgcggtga
      781 ttgtaagtgt gatgatgtct ggcagagcgt tgttccagtg gttctcgttg ctgcagatta
      841 ccggttgctc ggtttcaact tgttgcgagg cattcaatgg aactgatagc aacgtcgcca
      901 gcacaatacc caacaagatt gttcgcatcg acgttctccc gaagattatt gctcaaaatc
      961 ctctaacgct ctctccaccg cctccggttc tttctcgatc accgtgagaa gcacgcgcgc
     1021 ggcgacatcc accttcttgc cttgttccca cccttcaatc gtccgcgcag gcacgccgaa
     1081 gcggcgctcg aattcacgcg ggcttttcgc gacggatttg cggatcgctt tcacgcgctt
     1141 ggcagccatc ggctcgatgg ttcgcgtcgg caactcgatc ccgccgcgct tccacgccac
     1201 cgcttcgcgc aagccttctt cgatggcgag gccaagcttc gttctcttgc tcatcggttc
     1261 aactcctcca acatacgcag gatcgccttg cgatcatccg gcgaaagatc ttccttctcg
     1321 gccttggcgt aagcggtgat catgagaacg gtgtcgtccg ccatcatcac gtaatagatc
     1381 gcccgcccgc cgccgctctt gccgcgattg ccgatcctga agcgcgcctt gcgcacgcct
     1441 ttcaagcctt ggatcaacgc accggccaaa ggatcggccg caatcatccg ctccatctca
     1501 tcgacatccg ctgccgaagc cttcagcttc ttgatgtctt tgacgtagcg atgagtacgg
     1561 acgatcttta cgaagggcat aatacgctac cagcgtataa attgcaagag aaaatacgct
     1621 gtcagcgtat aagttcaaat gagtcaaaac gcctcaccgc cgaggagatg aacgcgctac
     1681 tgcgcgaaat ggaatccaca cccattccgg tcaatgcaac cacggccgcc cgacctatgt
     1741 ggagctgaag ctgtcggata tagaaaggtt gttcggccgc agatgaacga ttgccggcct
     1801 cgtttcgctt ccccgcttac ttcaacgcaa acgagttcag gaacgcgaga gacgccttgc
     1861 tgttttcccg gcctttcgga ccgatgaacg ttatctggaa ctgcctgttg cgaacgtaaa
     1921 agacgcgcaa atgcgccgtc tgattgttct ctttcacatc gatcagggca tcgcggccgg
     1981 tgatcttgcc gagcttataa ggcgtgacgc ggcgaatctt gcccttgatg ttgctggcgg
     2041 cgccggtgac ggcgccggta taggcggcat cgatggattt tgcggtgatg gaacgggctg
     2101 ggtaatcggc gacggcgacg aagaatcctc cgtcgcgcgt accgcaggaa tacgccgtca
     2161 gtttgacgtt gcccacggcg gtcgcaatat tcctgctgga ctgctgcgat tgacaatcga
     2221 acaccactgc gaagccccat tgagcggcgt tgagggcttc ggccc
//
LOCUS       DASWPU010000006         7430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_55074,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000006 DASWPU010000000
VERSION     DASWPU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55074"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1789)
                     /locus_tag="VGC27_00120"
     CDS             complement(<1..1789)
                     /locus_tag="VGC27_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0281015.1"
                     /translation="MQWKKILSGSASVLVLTLAAGAAFAQIDDQQSVETVVVTGIRAS
                     LEKGLEIKRASTQVVESIVAEDIGKLPDNNIVEALQRISGVQVTNRGSGQVGALYIRG
                     MPDVETTWNGRKIFTASGQFFSYQDMPATLVSQLDVYKTRAASQIETGIGGQLNISSY
                     RPFDFDGFKLSADVRGTYEELRGAFDPALSLLVSDRWQTRIGEIGVMANLSWSVVRFR
                     DESATPGALVPFQTETPTAGWTPLERIFNTDARASEALIWQPGLIEGLPQAPGSTIKI
                     NGVDTPYYLSRDAIFQSDLTGKRTRPNANIAVQWAPNSNSVYTLEVMYNGFRNETFNN
                     LFFSFVDWWGDFAGATPRIDPATSFTLFDGTNIIKTRHAGGVYGFNSGDLTSSKTNSF
                     VYALNAKWDISNRLKLAADASHQTSIYDTQFFAMRIDRSAPDIYVDFNSGGGYLAFNF
                     GDGQDLLTTPSAWNTAQLYDNANRNKGSASTLSFDGTYDLDGIAGVVKTVRFGVRYDD
                     RGAKEAYRQASDWHGQNLSTMPTEILYVNHDFFDGRAAVPDSWVNVDGRYIYTHRDEL
                     RTQWGGQLTDARLHEMFETFKIDEANTTAY"
     gene            2104..2904
                     /locus_tag="VGC27_00125"
     CDS             2104..2904
                     /locus_tag="VGC27_00125"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY0281016.1"
                     /translation="MKMPARFTRMNKLEPLGRSRPISKVLRLHGTIARDLGIAIMAGK
                     YRPGDLLTGEIASSERLEVSRTAYREAVRILAAKGIVESKPKVGTKVSAVHKWHWLDP
                     DVLAWVFESEPDLDLLYSLFELRDIVESAAAAMAAERRSETHLKLMRQAIDGMRRHTL
                     ATEAGRKADQDFHTTLLQATNNPFMISLTTGVNAAVSTTTMFKQRNRPLPRDPVPDHL
                     RVLEAVADKDPVRAHKAMSELVRLALQDTPVPRRPKKRATRRKAKPRA"
     gene            3212..4723
                     /gene="araA"
                     /locus_tag="VGC27_00130"
     CDS             3212..4723
                     /gene="araA"
                     /locus_tag="VGC27_00130"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEY0281017.1"
                     /translation="MTSMKQLEVWFVTGSQHLYGPETLDKVAEDSQKIAKALCAAPAI
                     PVQILFKPVLVSPDSITELALAANASPKCAGLIIWCHTFSPSKMWINGLKQLRKPALH
                     LHTQFNRDIPWSTIDMDFMNLNQAAHGDREHGFIWSRLRLNRKVVVGHWQEETVQQKI
                     GAWTRAAAAWLDWQGAKFARFGDNMREVAVTEGDKVSAQYKFGYSVSGYGVGELVKVI
                     NAVSEKKIDRLVEEYESLFKVAADLRKDGSRHKFVREAARIELGMRAFLKDGGFKGFT
                     TTFEDLHGLEQLPGLAVQRLMADGYGFGAEGDWKTAALLRAVKVMATDLKGGSSFMED
                     YTYHLNPVGKCVLGAHMLEVCPSIAERKPSLEVHPLGIGGKMDPARLVFNTPPGPATA
                     STLIDLGNRFRLLVNELDVISPKQPMPKLPVAQAVWKPRPNLEDACAAWIYAGGAHHT
                     AFSQAVTSEMLEDFASMADIEFLLIGKNTSVPEFRKELRSNEVYYALNRGWAI"
     gene            4740..6407
                     /locus_tag="VGC27_00135"
     CDS             4740..6407
                     /locus_tag="VGC27_00135"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEY0281018.1"
                     /translation="MGVHYTIGLDYGTNSVRALLADTSDGREIATAIWGYEHGHSGVV
                     LGRDPNLARQHPADYLKGAEIAIKQALAEAAKSVRGFAPQQVAGIGVDTTGSTPMPVD
                     ENGQPLAFHTDFADNQAALAWLWKDHTSFAEAAEITALGRDMRPQIMAKIGGVYSSEW
                     FYSKILHCLRSAPEVFDAAHAWIELADFVPAALTGTLAPDKFTAGICAAGHKAMYNEA
                     WGGYPDKEFLSRLDPKLGALRDRLTKRVRAVDSSAGGLTQEWAEKTGLPAGIPVAVGA
                     FDAHLGAIGAGVRPGTLVKIIGTSTCDITVKRNSEALADVPGLCGIVDGSVLPDFFGL
                     EAGQSAVGDIFEWFVGYVKPEGGSHAELSAGAAKLQPGESGLMALDWNNGNRTILVDQ
                     RLTGLLVGQTLYTTPAEIYRALIEATAYGALTIINRFEEYGVKIDQVVNCGGIAEKSP
                     LTMQIYADVTGRPMKISRSAQTPALGAAIAAAVVARVHADFASAQKAMTGLKPEVFTP
                     DPAAHAVYRELYAVYRTLHDAFGTEGWNGSLYNVMKTLIDIRNRARK"
     gene            6412..7104
                     /locus_tag="VGC27_00140"
     CDS             6412..7104
                     /locus_tag="VGC27_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEY0281019.1"
                     /translation="MLEDLKRQVCQANLDLVREGLVIQTFGNVSGIDRARRLAVIKPS
                     GVAYDAMKPEHMVVVSLESGAVAEGDLRPSSDTPTHLVLYRAFKDIGGITHTHSLYAT
                     AWAQARRPIPPYGTTHADYWHGEVPCTRPMMPQEIETDYEANTGEVIVEAFKGVDPMR
                     CPAVLVASHGPFTWGKDAAGAVHNAAVLEFVAKLASETMHINPNTEAMQSALLDKHFL
                     RKHGPKAYYGQK"
     gene            7129..>7430
                     /locus_tag="VGC27_00145"
     CDS             7129..>7430
                     /locus_tag="VGC27_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281020.1"
                     /translation="MNKGITAAAAASGPVFNSLDWFIVALFVAGMVAVVMYSMRKKAE
                     SGKDYFLSSRDSNWLQIGTSIFSSNIGSEHLVGLAGAGFATGMAMAHWEIQSWLILV"
BASE COUNT         1482 a         2285 c         2310 g         1353 t
ORIGIN      
        1 agtaggccgt ggtgttcgcc tcgtcgatct tgaaagtctc gaacatctcg tgcaagcggg
       61 cgtccgtcag ctgcccgccc cattgcgtcc ggagctcatc gcgatgggtg tagatatagc
      121 gaccatccac attgacccac gaatccggca cggcggcgcg gccgtcgaag aagtcgtggt
      181 tcacatagag aatctcggtg ggcatcgtcg acaggttctg cccatgccag tcgctcgcct
      241 gcctgtaggc ctctttggcg ccacggtcat catagcgcac gccgaagcgg accgtcttga
      301 cgacgccggc gatcccgtcg agatcgtaag tgccgtcgaa ggacagcgtg gaggcggagc
      361 ccttgttgcg attggcgttg tcgtatagct gggccgtatt ccaggccgac ggcgtcgtta
      421 ggagatcttg gccgtcgccg aaattgaagg ccagatagcc gccgccggag ttgaagtcga
      481 cgtagatatc cggggccgac cggtcgatgc gcatggcgaa gaactgggtg tcgtagatgc
      541 tggtctgatg ggaggcgtcg gcggccagtt tcaggcggtt gctgatatcc cacttcgcgt
      601 tcaacgcata gacaaagctg ttggtcttcg aggaggtgag gtcgccgctg ttgaatccgt
      661 agacgccgcc cgcatggcgc gtcttgatga tgttcgtgcc gtcgaacagc gtgaaactgg
      721 tcgccggatc gattcttggc gtggcgccgg caaagtcgcc ccaccagtcg acgaaactga
      781 aaaacaggtt gttgaaggtc tcgttgcgga agccgttgta catgacttcg agcgtgtaca
      841 cggagttgct gttaggcgcc cactggaccg cgatgttcgc gttgggacgc gtgcgcttgc
      901 cggtgagatc gctttggaag atggcatcgc gggacaggta atacggcgta tcgacgccgt
      961 tgatttttat ggtcgagccg ggcgcctgcg gaagaccttc gataaggccg ggctgccaga
     1021 tgagcgcttc cgacgccctg gcatccgtat tgaaaatgcg ttcgagcggg gtccaaccgg
     1081 cggtcggcgt ttctgtctgg aacggcacga gcgcgcccgg ggttgcgctc tcgtcgcgga
     1141 accgcacgac cgaccaactg aggttggcca tgacgccgat ctcgccgatg cgcgtctgcc
     1201 agcgatcgct gacaagcagg ctgagggcgg gatcgaacgc ccctctcaac tcctcgtagg
     1261 ttcctcgaac gtctgcggac agcttgaagc cgtcgaaatc gaacgggcgg taggatgaaa
     1321 tgttgagctg accgcctatg ccagtttcga tctggctggc cgcgcgcgtc ttgtagacgt
     1381 cgagctggct gaccagcgtc gccggcatat cctgatatga gaagaactgg ccggatgccg
     1441 tgaagatctt gcgaccgttc cacgtcgttt caacgtcggg cataccgcgg atatataggg
     1501 cgccgacctg gccgctgccg cgattggtca cctgcacgcc ggaaatgcgc tgcagcgctt
     1561 cgacgatgtt gttgtcaggc agtttgccga tgtcttcggc gacgatcgac tcgaccacct
     1621 gagtcgatgc gcgcttaatc tccaaaccct tttccaagct ggcgcggatg ccggtgacga
     1681 ccacggtttc gacgctttgc tggtcgtcaa tctgcgcaaa cgctgcgccc gccgccaacg
     1741 tcaaaacaag taccgacgca gaaccgctca gtattttctt ccactgcata acttccctcc
     1801 ctgcagtcgt ctcgttcggg cgctgattca cccaaaggcg acctcgataa ttactcagac
     1861 aaataggcta tttaatttcg cagacaaata caagatgcag ctggcgcagg gagattcgat
     1921 ggcccgccca tgtggctaat tcgcaccagc ctgccgaggg acacgcagaa gtatgtcggg
     1981 atcgatggcc aggcgatggc gccttcgacc gccgcaacgg cccgggaacg gcctgcggaa
     2041 gcgggccgcg actcacaaaa aggcggattg gacttgcttt ccaatgggct atagtaggag
     2101 cccatgaaaa tgcccgctcg gttcacccgc atgaacaaac tcgaaccact tgggcgttcg
     2161 cgcccgatca gcaaagtgct gcggttgcat ggcacgatcg cgcgcgatct tggtatcgct
     2221 atcatggccg gcaagtatcg gccgggggac cttttgacgg gcgaaatcgc ttccagcgag
     2281 cgcctggaag tctcgcgcac ggcctatcgc gaagccgttc gtattctcgc cgccaagggc
     2341 atcgtcgaat cgaaacccaa ggtcggcacg aaggtaagcg ctgtgcacaa gtggcactgg
     2401 ctcgatccgg acgtgctggc ctgggtgttc gaatccgaac cggacctcga cttgctttat
     2461 agcctgtttg aattgcgcga tatcgtcgaa tccgccgccg cggccatggc cgcggaacga
     2521 cgctcggaga cgcatctgaa gttgatgcgg caggccatcg acggaatgcg gcgccatact
     2581 ttggcgaccg aagccggccg taaagccgac caggattttc acacgacctt gctgcaagcc
     2641 accaacaatc ctttcatgat ttccctgacc acgggcgtca acgccgccgt cagcacaacc
     2701 accatgttca aacagcgcaa tcgtccgttg ccgcgcgacc ccgtccccga ccaccttcgg
     2761 gtgctcgaag ccgtcgcgga caaggatccg gtgagagccc acaaggcgat gagtgagctg
     2821 gtccgcctgg cgctccagga tacgcccgtg ccgcgccggc cgaaaaaaag agcgacccgg
     2881 cgcaaggcca agccgcgcgc gtgacaggtc gcaggcaacg ctccggcggt gcggcgaacg
     2941 cgcgcattac ccagccgccc acggtccgta tttccaaagc agcgatagaa gcgtgccgcg
     3001 gcccctgcgt cacgatgtaa agcggacgcg ccataaagtc gggggcgcag gaacatgcag
     3061 gaattttgcg ggcttcaggc gcccggcgga tgccccggtt aggggccgcc acccctcatg
     3121 ccggcgggcg atactatttg tctgattttt gagatgcggc catttcggaa ttgtgataac
     3181 aagcccgcag taacatcggg acgttttcgc catgacatcc atgaagcagc ttgaagtctg
     3241 gttcgtcacc ggcagccagc atctgtacgg gccggaaacg ctcgacaagg ttgccgagga
     3301 ttcgcagaaa atcgccaagg cgctctgcgc cgcgccggcg attccggtgc aaatcctctt
     3361 caaacccgtt ctggtgtcgc cggattccat caccgagctt gcgctggcgg cgaacgcgtc
     3421 gcccaaatgt gctggtctca tcatctggtg tcacacattc tcgccttcga aaatgtggat
     3481 caacggcctc aagcaactac gcaagccggc gcttcatctg cacacccaat tcaaccgcga
     3541 catcccatgg tcgacgatcg acatggattt catgaacctc aaccaggcgg cccatggcga
     3601 ccgcgagcac ggtttcatct ggagccgtct gcggctgaac cgcaaggtcg tcgtcggcca
     3661 ttggcaggaa gagacggttc agcagaagat cggcgcctgg acgcgcgccg cggcggcctg
     3721 gctcgactgg caaggcgcga aattcgcccg cttcggcgac aatatgcgcg aagtggccgt
     3781 caccgaaggc gacaaggttt cggcgcagta caagttcggc tactcggtca gcggctacgg
     3841 cgtcggcgag ttggtgaaag tcatcaacgc ggtttccgaa aagaagatcg acaggctcgt
     3901 cgaagaatat gaaagcctgt tcaaagtggc ggccgatctt cgcaaggacg gaagccgcca
     3961 caaattcgta cgcgaagccg cccgcatcga actcggcatg cgcgccttcc ttaaggatgg
     4021 aggcttcaag ggtttcacca ccacgttcga ggatttgcac gggctcgaac agcttcccgg
     4081 cctcgccgtg cagcgcctga tggcggacgg ctacggcttc ggcgcggaag gcgactggaa
     4141 gacggcggcg ctgctgagag ccgtaaaggt catggcgacc gatctcaagg gcggttcgtc
     4201 gttcatggaa gattacacct atcatctcaa tccggtgggc aagtgcgtgc tgggcgcgca
     4261 tatgctggaa gtctgtccgt cgatcgcgga gcgcaagcca tcgctggaag tgcatccgct
     4321 gggcatcggc ggcaagatgg atccggcgcg cctggtgttc aatacgccgc caggtccggc
     4381 cacggcctcg acgctgattg atttgggcaa ccgtttccgt cttctggtga acgaactcga
     4441 tgtcatctcg cccaaacagc cgatgccgaa actgccggtg gcgcaagcgg tatggaaacc
     4501 gcggccgaac ctcgaagacg cctgcgccgc gtggatttac gcgggcggcg cccatcacac
     4561 cgcgttcagt caagcggtca ccagtgaaat gctggaggat ttcgcgagca tggccgacat
     4621 agaattcctg ttgatcggca agaacacgtc cgtaccggaa ttcaggaaag aactgcgcag
     4681 caacgaagtc tattacgcgc tgaaccgcgg ctgggcgatc taaatcgggt tcattcgata
     4741 tgggcgttca ttacacgatc ggtctcgact acgggacaaa ctcggtccgc gcgctgctgg
     4801 ccgacacttc cgacggcagg gaaatcgcca ccgccatctg gggctacgag cacggccatt
     4861 ccggcgtcgt cctcggccgc gaccccaatc tcgcgcgcca gcatcccgcc gattacctga
     4921 agggcgcgga aatcgccatc aagcaggcgc tcgccgaggc ggcaaagtcc gtgcgcggct
     4981 ttgcgccgca acaggtggcg ggcatcggcg tggacacgac aggttcgacg cccatgccgg
     5041 tggacgaaaa cggccagccg ctcgcgtttc acacagactt cgccgacaac caagccgcgc
     5101 tcgcctggct gtggaaagat cacacctctt tcgcggaagc ggcggaaatc accgcgctgg
     5161 ggcgcgacat gcgcccgcag atcatggcca agatcggcgg cgtctattcg agcgaatggt
     5221 tctacagcaa gattcttcac tgtctgcgca gtgcgccgga ggtgttcgac gccgcccatg
     5281 cgtggatcga gctggccgat ttcgttcccg ccgcgctgac cggcacgctg gcgccggaca
     5341 agttcaccgc cggcatctgc gccgccggcc acaaggcgat gtacaacgaa gcctggggcg
     5401 gttacccaga caaggaattc ctctcgcgtc tcgatcccaa gctcggcgcc ttgcgcgacc
     5461 ggctgaccaa acgggttcgc gcggtggatt ccagcgccgg cggcttgacc caagaatggg
     5521 ccgaaaagac cggcctgccg gcgggcattc ccgtggcggt gggggccttc gatgcgcatc
     5581 tcggcgccat cggcgcgggc gtccggcccg gcacgctggt caagatcatc ggcaccagca
     5641 cctgtgacat caccgtgaaa cgcaacagcg aagcgcttgc cgacgtgccc ggcctgtgcg
     5701 gcatcgtcga cggcagcgtg ctgccggact ttttcggtct cgaagcgggg caatcggccg
     5761 tcggcgacat tttcgaatgg ttcgtcggct atgtgaaacc ggaaggcgga tcgcacgcgg
     5821 aactctcggc cggcgctgca aaactgcaac ctggcgaatc cggcctgatg gcgctggact
     5881 ggaacaatgg caaccgcacg attctggttg atcagcgcct caccggcctc ttagtcggcc
     5941 agacgctgta caccacgccg gcggaaatct atcgcgccct gatcgaagcg accgcctatg
     6001 gcgcgctgac gatcatcaac cgcttcgagg aatacggcgt gaagatcgac caagtcgtca
     6061 attgcggcgg catcgcggaa aaaagcccgc tcaccatgca gatctatgcc gacgtgacgg
     6121 gccggccgat gaaaatatcg cgctcggcgc agacgccggc gctgggcgcg gcgatcgcgg
     6181 ccgcggtggt ggcgcgcgtt catgccgact tcgcctcggc gcaaaaagcg atgaccgggc
     6241 tgaagccgga agtcttcacg cccgatccag ccgcgcacgc cgtctatcgc gaactctatg
     6301 cggtctaccg cacgctgcac gacgctttcg gcaccgaagg gtggaacggc agtctctaca
     6361 acgtgatgaa gacgctgatc gatatccgca accgcgcccg caagtgacgc catgctcgaa
     6421 gacctgaaac ggcaagtctg ccaagccaac ctcgatctcg tccgcgaagg actggtgatc
     6481 cagaccttcg gcaatgtcag cggcatcgac cgcgcgcgca ggctggccgt catcaagccg
     6541 tccggcgtgg cctacgacgc gatgaagccg gaacatatgg tcgtcgtgtc gctggagagt
     6601 ggtgcggtgg cggaaggtga tctcaggccc agttccgaca cgccgacgca tctggtgcta
     6661 taccgtgcgt tcaaggacat cggcggcatc acccacacgc acagcctcta tgccaccgcc
     6721 tgggcgcagg cccggcgtcc gattccgccc tacggtacga cccacgcgga ttattggcac
     6781 ggcgaggtgc cttgcacccg gccgatgatg ccgcaggaaa tcgagaccga ttacgaagcg
     6841 aacaccggcg aggtgatcgt cgaggctttc aaaggtgtcg atccgatgcg ttgcccggcc
     6901 gtgctggtgg cgagccatgg gccattcacc tggggtaagg acgccgccgg cgccgtgcat
     6961 aacgcggccg tgctggagtt cgtagcaaag ctcgccagcg agacgatgca catcaatcca
     7021 aacaccgaag cgatgcagtc cgcgctgctc gacaagcact tcctgcgcaa gcacggaccg
     7081 aaagcgtatt acggacaaaa atagagaagc cggaggggac accatcacat gaataagggc
     7141 atcaccgccg cggccgcggc atctggaccc gtgttcaatt cgctggactg gttcatcgtc
     7201 gcattgttcg tcgccggaat ggtggcggtc gtcatgtatt ccatgcggaa gaaagcggag
     7261 tccggcaagg actatttcct ctccagccgc gacagcaact ggctgcagat cggcacctcg
     7321 atcttctcct ccaacatcgg ctccgagcat ctggtgggcc tggccggcgc gggcttcgcc
     7381 accggcatgg cgatggcgca ttgggagatc cagtcctggc tcatcctcgt
//
LOCUS       DASWPU010000007         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_73430,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000007 DASWPU010000000
VERSION     DASWPU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73430"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VGC27_00150"
     CDS             <1..294
                     /locus_tag="VGC27_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEY0281021.1"
                     /translation="SVISPEGCASILWRRPEKNQDAAAAMRLTAQDLLGLGLIDAIVA
                     EPMGGAHRAVEEAINNVGEQIAHGLSELSALAPDELRRQRRDKFLAMGRDLAK"
     gene            389..1309
                     /gene="rssA"
                     /locus_tag="VGC27_00155"
     CDS             389..1309
                     /gene="rssA"
                     /locus_tag="VGC27_00155"
                     /inference="COORDINATES: protein motif:HMM:NF007623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HEY0281022.1"
                     /translation="MPRRKIGLALGSGAARGWSHIGVVEALLEAGIVPEVVCGTSIGA
                     LVGAAYVTDKLAALRTRVETFGRRDMVAMVDVRFSGGGLIEGRRIGAFLRDLGISAPI
                     ESLHKRFAAVVTDLATGREVWLEKGPIDEAVRASISVPGVFSPVRLDGAWYLDGALVN
                     PVPVSVCRALGADVIIAVNLSGDLVGRRFTDEGETAASAKTATARREFVGRLIDGLPP
                     SLKKQAGFVAKKLLQPEAAPGYLDVLANALNIMQDRVTRTRLAGEPPDVLLSPRLAGM
                     RWTEFHRGSAAIAAGRTAVQKALPVLREYL"
     gene            complement(1398..2342)
                     /locus_tag="VGC27_00160"
     CDS             complement(1398..2342)
                     /locus_tag="VGC27_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEY0281023.1"
                     /translation="MRILSLLRVTAAFALAVAVAAPATAETIRGKTQRKEAQIPVCSH
                     RIGSIAVYEPENKWWVDLKLESPEALLKVFVMRSGCFSLVDRGKGFSAVQQERELASQ
                     GQLRQGSNMGKGQIKTADYVLVPDVVSRNADSGGSAIGGILGGFIGGGVGAVVSNIRI
                     SSSTADVVLTVTDVRSSEQVAMEEGHGSKSDLGFGVGGGGIWGGGGGALGVSSYQNTQ
                     IGQVVTLAYIDAYTKLVRQMGGISDNASMDNAEQAVTVVKQASLYDRPGGSGKVVRKL
                     DVGMMLYPTGIKDGVWWEVKDEMGNSGWVSSLTLELAK"
     gene            complement(2516..>3935)
                     /locus_tag="VGC27_00165"
     CDS             complement(2516..>3935)
                     /locus_tag="VGC27_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY0281024.1"
                     /translation="QPVLVCTTSIEKSEQLSELLKKRKIKHNVLNARYHEQEAAIVAQ
                     AGVSGTVTIATNMAGRGTDIQLGGNADMRIRTELGGIVDQDELTRRAAEIRAEVDADK
                     EKVIAAGGLYIVGTERHESRRIDNQLRGRSGRQGDPGASKFFLSLQDDLMRIFGSDRM
                     ESILTRLGLEEGEAIIHPWVNRALEKAQQKVEARNFEIRKNILKYDNVLNDQRKVIFE
                     QRKEIMSADDVSDQIGEFRLEVIAELVERHIPPKAYAEQWDATGLHDEVESIFGIDLP
                     IVDWTHEEGIADEEVRERITKIVETRAADRAANFGPEIMRYAEKAILLQTLDHNWRDH
                     IVNLDYLRQYVGLRGYGQRDPLNEYKGEAFELFEGLLSKMRIDVVRQLMHIAISVEQP
                     PPEMPTAVPLQMREVKIDPLTGEDQMEAGAAHRAGQPRHNRPRDVAVDPNDPSTWGKV
                     QRNAPCPCGSGRKFKHCHGALV"
BASE COUNT          641 a         1228 c         1244 g          822 t
ORIGIN      
        1 tcggtgatat cgccggaagg ctgcgcttcg atcctgtggc gccggcccga aaagaaccag
       61 gacgccgccg ccgccatgcg cctgaccgcg caggatcttt tggggcttgg cttgatcgat
      121 gccatcgtgg cggaaccgat gggcggggcc catcgcgcgg ttgaagaagc catcaacaat
      181 gtcggcgagc agatcgccca cggactgagc gaactgtcgg cgctggcgcc cgacgagttg
      241 cgccgccagc gccgcgacaa gtttctcgcc atgggccgcg atctggccaa atagaagaaa
      301 cccgcgcgct tgggttagat agactcatgc gcccgccgcc atcgagcgtt atagtcggtt
      361 tgtccatcgg cgagggagtc agtgcggcat gccgcgtcgg aagatcggac ttgctcttgg
      421 cagcggtgcg gcgcgagggt ggtcgcatat cggggtcgtt gaagcgttgc ttgaagccgg
      481 catcgtgccg gaagtcgttt gcggcacttc gatcggagcg ttggtggggg cggcctatgt
      541 aacggataag ctggcggcat tgcgcacgcg cgtagaaacc ttcggccgcc gcgacatggt
      601 tgccatggtg gacgtgcgct tttcgggggg aggcctgatc gagggccggc gcatcggtgc
      661 gttcctgcgc gacttgggca tttcggcgcc gatcgaatct ctgcacaagc ggttcgctgc
      721 cgtggttacc gatctcgcaa ccggccgcga ggtttggctg gaaaaaggac ccatcgacga
      781 agcggtgcgc gcctcgatca gcgtccccgg cgtctttagt ccggtgcggc ttgacggcgc
      841 ttggtatctc gatggagccc tcgtcaatcc ggtgccggtg tcggtgtgcc gcgccctcgg
      901 cgccgacgtc attatcgcgg tcaatctcag cggcgatctg gtcggcaggc gctttaccga
      961 cgagggcgag accgccgcgt cggcgaagac tgcgacggcg cggcgggaat ttgtgggccg
     1021 ccttatcgat ggacttccgc ccagcctaaa aaaacaggca ggctttgtag cgaagaagct
     1081 gctccagccg gaagccgctc cggggtacct cgacgtcctg gcgaatgcgc tcaacatcat
     1141 gcaggatcgc gtcacgcgca cgcgccttgc cggcgagcct ccggatgttc tattgagtcc
     1201 gcgccttgcc ggtatgcgct ggacggagtt tcatcgcggc tccgcggcga ttgccgccgg
     1261 gcgaaccgcg gtgcagaagg cgctgcccgt gcttcgcgaa tatctttagc cgccgcggtg
     1321 tattaatcga ttgcgtggat tgcccacgaa aaagggggcc tcgggccccc tttcccggcg
     1381 cgcgttgccg tcgcgttcta tttcgccagc tccagcgtga gcgaagacac ccaaccgcta
     1441 ttgcccattt catctttgac ttcccaccac acgccatctt tgatgcctgt ggggtagagc
     1501 atcatgccga cgtcgagctt gcgcacgact ttgccgctgc cgccgggccg gtcgtataga
     1561 ctggcctgtt tcaccacagt gaccgcttgc tcggcattgt ccatcgaagc gttgtccgag
     1621 attccgccca tctgcctcac cagcttggtg taggcgtcga tataagcgag cgtcacgacc
     1681 tgaccgattt gcgtgttctg gtagctggaa acgcccagcg caccgccgcc gccgccccaa
     1741 atcccgccgc cgccgacgcc gaagcccaga tcgctttttg agccatggcc ttcttccatc
     1801 gccacctgtt cggaggagcg cacgtcggtg accgtcagaa cgacatccgc cgtgctgctg
     1861 gaaatcctga tattgctgac gaccgctccc acgccgccgc cgatgaaccc gcccagaatg
     1921 ccgccgatgg ccgagccgcc cgaatccgcg ttccgcgaaa ccacgtcggg cacgagcacg
     1981 tagtccgccg ttttgatctg gcctttgccc atgttggagc cttgacgcaa ctggccctgg
     2041 ctggcgagtt cgcgctcctg ctggaccgcc gaaaagccct tgccgcgatc gaccagcgag
     2101 aagcagccgg accgcatgac gaagaccttg agcagggctt cgggcgattc cagcttcagg
     2161 tccacccacc atttattctc gggttcgtaa accgcgatgc ttccgatcct gtggctgcag
     2221 accgggatct gcgcttcctt ccgctgtgtc ttgccgcgaa tggtttccgc cgtggccggc
     2281 gcggcaacag ccaccgccag cgcgaacgct gcggtcacgc gcagcaggct caaaatgcgc
     2341 atttcaattt tctcctgtgc ttccccaatg tgacgcggcc gtcgcggccg cgcctggcgc
     2401 cctctctatt ccaagactag cgcaagactc taatccggta aacgccggaa agcgccgtcg
     2461 aacactaaaa gatcgcgtcg agacgaagga tatctatcct caaggaaagt aaagttcaga
     2521 ccagcgcacc gtggcagtgt ttgaacttgc gccctgaacc gcaggggcag ggtgcgttgc
     2581 gttgcacctt gccccaggtg cttggatcgt tgggatcgac ggccacgtcg cgcggcctat
     2641 tgtggcgcgg ttggcccgcg cgatgcgcgg cgcccgcttc catttggtct tcgccggtga
     2701 gcggatcgat cttgacttcg cgcatctgca agggcacggc cgtcggcatt tccggcggcg
     2761 gctgctccac gctgattgcg atgtgcatga gctggcggac gacgtcgatg cgcatcttgc
     2821 tgagcaggcc ttcgaacagt tcgaaagctt cgcctttgta ttcgttcagc gggtcgcgct
     2881 gtccgtagcc gcgcaggccc acatattgcc gcagataatc gagattgacg atgtgatcgc
     2941 gccaattatg gtccagcgtc tgcaacagga tcgccttttc ggcgtagcgc atgatctcag
     3001 ggccgaaatt cgcggcgcga tcggcggccc gggtttccac gatcttggtg atgcgttcgc
     3061 gcacttcttc gtcggcgatg ccttcctcat gtgtccaatc gacgatgggc aaatcgatgc
     3121 cgaagatact ctccacttcg tcgtgcaggc ccgtcgcgtc ccactgctcg gcataggctt
     3181 tcggggggat gtggcgttcg accagttcgg cgatgacttc caggcggaat tcgccgatct
     3241 gatcgctgac gtcgtctgcg gacatgatct ccttgcgctg ctcgaagatc accttgcgct
     3301 ggtcgttgag gacgttgtcg tatttcagga tgttcttgcg aatttcgaag ttgcgcgctt
     3361 cgactttctg ctgcgccttc tccagagccc tgttgaccca gggatggatg atcgcttcgc
     3421 cctcttcgag gccgagccgc gtcaggatcg actccatgcg gtccgatccg aagatgcgca
     3481 tcagatcgtc ctgcaggctg aggaagaatt tcgaggcgcc ggggtcgccc tggcggccag
     3541 aacggccgcg cagctggttg tcgatgcgcc ggctttcgtg acgctcggtg cccacgatgt
     3601 aaaggccgcc cgcggcgatc accttctcct tgtcggcgtc gacctcggcg cggatttcgg
     3661 ccgcgcgccg ggtgagttcg tcctggtcga cgataccgcc gagttcggtc ctgatccgca
     3721 tgtcggcatt gccgccgagc tgaatgtcgg tgccgcggcc ggccatgttg gtggcgatgg
     3781 tgacggtgcc ggaaacgccg gcttgggcca cgattgcggc ttcctgctcg tgatagcgcg
     3841 cgttcaacac attatgcttg atcttgcgct tcttcagcag ttcggagagt tgctcggact
     3901 tttcgatgga ggtggtgcag accagcaccg gctgg
//
LOCUS       DASWPU010000008         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_18451,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000008 DASWPU010000000
VERSION     DASWPU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18451"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="VGC27_00170"
     CDS             complement(<1..1201)
                     /locus_tag="VGC27_00170"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY0281025.1"
                     /translation="MSNTVARSARLLPFLFLAWTMADPSFAADVSPSKDDLLAGFSQP
                     PAEAKPRVWWHWMNGNITKEGIKKDLDWMKRIGIGGVDAIDAAIDSPQIVDKRLAYMT
                     PEWKDAFLYAVKLADKYGMEFSIDSSPGWSVSGGPWVKPEQAMKKAVWSTTVLEGGKA
                     FHGRLPQPPDNVGPIQNALFRGDPTPMPKTAATKFYRDAIVVAYKQPVDEPVVEEAAS
                     NGGNIDVAALTDDDLENGMTLKAPTQDSDVWISLKYARKTRIQGWTLGLLADRALGYA
                     ASLEASDNGKNWRHVADMPPGAVAVRLLLVQQTISFNPVTARYFRVVLKPAKPIPRSN
                     RPLSAAAPGYDGAADTPPEPPRSYKLVQLQLRADATVHEFEMKALYATPIRDFYSLAT
                     TSEYAPGS"
     gene            1490..>1674
                     /locus_tag="VGC27_00175"
     CDS             1490..>1674
                     /locus_tag="VGC27_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281026.1"
                     /translation="METTVPGLNRRDFVRIAGSSGLVLPFAEAEAMSRGRKALASASR
                     ENGLAIPDDGWHLWIDQ"
BASE COUNT          303 a          503 c          531 g          337 t
ORIGIN      
        1 cgctgcccgg cgcgtattcg gacgtcgtgg cgaggctgta gaaatctctg atcggcgtgg
       61 cgtacaacgc cttcatctcg aattcgtgca ccgtggcatc ggcgcggagc tggagttgca
      121 ccagcttgta gctgcgcggc ggctccggcg gggtgtcggc cgccccatcg tagcccggcg
      181 ccgccgcact gagggggcgg ttgctgcggg gaatgggctt ggccggcttc aacaccacgc
      241 ggaaatagcg cgcggtgacg gggttaaagg agatcgtctg ctgcaccagc aacaggcgca
      301 ccgccaccgc tcccggcggc atatcggcga cgtgacgcca gtttttgccg ttgtcgctgg
      361 cttccaggct ggcggcatag cccagggcgc gatccgccaa cagcccgagg gtccagccct
      421 ggatgcgcgt cttgcgggcg tatttgaggc tgatccagac atcgctgtcc tgggtcggcg
      481 ccttgagcgt catgccgttc tcaagatcgt cgtcggtcag ggcggcgaca tcgatgttgc
      541 cgccgttgga ggcggcttct tccaccacgg gttcatcgac cggctgcttg taggcgacga
      601 cgatggcatc gcgatagaat ttcgtcgccg ccgtcttggg catgggggtg ggatcgcccc
      661 ggaacagagc attctggatg gggccgacat tgtcgggcgg ctgcggcagg cggccgtgga
      721 aggctttgcc gccttcgagg acggtcgtgc tccacaccgc cttcttcatc gcctgctccg
      781 gcttgaccca aggcccgccc gacacgctcc agcccggcga ggagtcgatg gagaattcca
      841 tgccgtattt gtcggccagc ttcaccgcat agagaaaggc gtctttccat tccggcgtca
      901 tataagcgag gcgcttatcg acaatctgcg gcgagtcgat ggcggcatcg atggcatcca
      961 ctccgccgat gccgatgcgc ttcatccaat cgaggtcctt cttgatgcct tccttggtga
     1021 tgttgccgtt catccagtgc caccacaccc gcggcttcgc ctcggcggga ggctgactga
     1081 agccggccag aagatcgtcc ttggatggag aaacgtcagc ggcaaaggag ggatcggcca
     1141 tcgtccaggc caggaacagg aaaggcagaa gccgcgcgga gcgtgctacg gtgttgctca
     1201 tgtctgaaac ccctcttaac ggacgccctc ggcatccttg tttcttgaca gaaggtcgtc
     1261 tggaattgat tgaatttgac gtttaatgtt ctatgttgcg cactttagat catttatgct
     1321 aacccaacaa cagggcaggc aggataactg ctcacatttg cgagcgcctt cgtgacgcag
     1381 agcgctccgg ggaagaaacg atgggcaaac tgggccggcc aatccagacc agaaccttct
     1441 ttgtcgatcc ttcttcgatt cgtatcaaac tgtctcaacc gcaaaacaga tggagacgac
     1501 cgtgccgggt ttgaaccgcc gcgatttcgt tcgcattgcc ggatcgagcg ggctagtgct
     1561 tccgttcgca gaggccgaag ccatgagccg gggcaggaaa gccctcgcct cggcgtcccg
     1621 ggaaaacggc ctggccatcc ccgatgacgg ctggcacctg tggatcgacc aaaa
//
LOCUS       DASWPU010000009         4975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_12370,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000009 DASWPU010000000
VERSION     DASWPU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12370"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1825
                     /locus_tag="VGC27_00180"
     CDS             182..1825
                     /locus_tag="VGC27_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281027.1"
                     /translation="MSGKVAAAALFVAAAAHGQSAGPPPALTPLYQIRFERWSEIDEK
                     DYGRFIAELGASGCATVDACLHDARNPFRGTDPPGTVFASDCADFPYVLRFYFAWKRG
                     LPFSYESSVRPRGATRDLRYTPYGNSVAGRTAPAGGNGTAILRTLRDAISTATYRIHP
                     NLEEPYQQDFYSPALAPGSIRPGTVIYDPNGHLAVVWRTDPDGRIHYFDAHPDHSVTR
                     GFYDLRFARSSPGMGAGFKNWRPQALTGAVKQADGTYSGGRIELAANKDIADFSDEQY
                     FGNGPRPAENSQWKTGAFTLNGETVGYYDYVRAKLAGGRLSFDPLREVADMVDSNCAD
                     LHYRVQAVELAVQAGLDKQPEPERLPPNIYGTEGDWETYSTPSRDARLKTAFKELRDL
                     AERFMRMADSRDKKLAYDGKDLAADMLAVYDRRAAQCRITYARSDGSRFTIGYEEARR
                     RLFEMSFDPYQCVERRWGASSGEEFAPCPDTTLKTAWYEAERNLRNQIDRTYDARMDF
                     TLAELKTPGPGKGVPAPPDTDVRAYLLSRLRPVETASEK"
     gene            complement(2243..3394)
                     /locus_tag="VGC27_00185"
     CDS             complement(2243..3394)
                     /locus_tag="VGC27_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281028.1"
                     /translation="MIPKKLHAIAMALSLGAVTVVAGSAFTATSAQAAARAAVGNPLN
                     AAKAAMAAGNCNAAMAKVREAQAVGGLSAEEQNYVTAMKNYIASASKGACGADTAIGA
                     KAKFEADYRAGRYRDAINDADLLRKAGALDGPTMVFIAQAYYRLGDFKGCARYASEHT
                     SAGQEMLKWQMTCAAKIGDDALMRSAAEQLVAVNGTPQNWTQLLKLVEAAKALSDHQT
                     LDINRIKLLTGLLKGDSDYFLLATLAIQLGFPSEAVAVIQKGFDAKVLSGDRANRLMN
                     LAKTSTASDLANLPKTVAAANKAKSGDLLVKLGENYCGMGRYKDAVDAVKAGIDKGVS
                     DEDNAYTRLGQALYGAGQKDAALKAFAKAKNKPNGEMIARLWSLYVRGH"
     gene            complement(3482..3886)
                     /locus_tag="VGC27_00190"
     CDS             complement(3482..3886)
                     /locus_tag="VGC27_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0281029.1"
                     /translation="MKAEDDVMLDINMTPLIDVLLVLLTLLIITLPIQTHAVKLDMPQ
                     PNPNPPQIQPIVVNLVVDFDGSILWNGTAVDRGTLASYFQNASQQDPQPEIHLNPNKL
                     ARYDIVAKVLADAQRLGVKKIGFTGLDQYMTQ"
     gene            complement(3930..4376)
                     /locus_tag="VGC27_00195"
     CDS             complement(3930..4376)
                     /locus_tag="VGC27_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0281030.1"
                     /translation="MAALNVQADAGHDEAELNATINVVPLVDIMLVMLIIFLVTIPVI
                     IKTVPMALPNARNIATITKPENIVISVDRNENIYWNNAPVEYSDLRGRFEAALIRSVH
                     SGTPLPEVHIRGDASVRFQALGRVILACQMAGVPKVGFITEPRNIE"
     gene            complement(4415..>4975)
                     /locus_tag="VGC27_00200"
     CDS             complement(4415..>4975)
                     /locus_tag="VGC27_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0281031.1"
                     /translation="SIGTWYIFFTKYLDQSRILNQARTVERRFWTSGTINEGIDRLPK
                     TSMFRAIAEAGVKAAAGGTSLVSLNDWIAMSLSRQLEDATARLQGGVTFLASVGSVAP
                     FVGLFGTVWGILQALIAIGVAGQASIDKVAGPVGEALIMTALGLAAAVPAVLLYNWLV
                     RRNKVITEKLRGFAGDLQTYLITKGK"
BASE COUNT          907 a         1607 c         1511 g          950 t
ORIGIN      
        1 gcaaaccgct tcacactttg ccggattgcg ctctagcttt ccgccggaga cagaaactcc
       61 gcccatcccg ggaagattat ggtgaacagg ccggctctac catatgcgcc gccgcggatc
      121 gcttcaggcg gcgcttgacc cgtcacaact agcctatacc gttcggggta tgggtagggg
      181 attgagcgga aaagttgcag cggcggcgct ttttgtcgcg gccgcggcgc atggccaaag
      241 tgcgggaccg ccgcccgcgc tcaccccgct ttaccagatc cgcttcgagc gctggagcga
      301 gatcgacgag aaggactatg ggcgctttat cgccgagctt ggcgcttccg gctgcgccac
      361 ggtcgatgcc tgcctgcacg atgcgcggaa cccgttccgc ggcaccgatc cgccgggaac
      421 cgtgttcgct tccgactgcg ccgattttcc ctatgtgctg cgcttctact tcgcctggaa
      481 gcggggcctg cccttctcct acgaaagcag cgtccggccc cgcggcgcga ccagagacct
      541 gcgctacacc ccctacggca actcggtcgc cggccgcacg gcgcccgccg gcggcaacgg
      601 caccgcgatc cttcggacgc tgcgcgatgc gatatcgacg gccacctatc gcatccatcc
      661 caatctggaa gagccttacc agcaggattt ctattctccc gccctcgcgc cgggctcgat
      721 ccgccccggc acggtgatct acgacccgaa cggccatctg gccgtggtgt ggcgcaccga
      781 tcccgacgga cgcatccatt atttcgacgc ccatcccgat cattcagtga cgcgcggctt
      841 ttacgatctg cgcttcgcgc ggtcctcgcc gggcatgggc gcgggcttca agaactggcg
      901 gccgcaggct ctgaccggcg cggtcaaaca ggccgacgga acgtatagcg gcggccgcat
      961 cgagcttgcc gccaacaagg acatcgcgga cttttccgac gagcagtatt tcggcaacgg
     1021 gccgcggccg gccgaaaact cccagtggaa gacgggcgct ttcacgctca acggcgaaac
     1081 cgtcggctat tacgactacg tgcgcgccaa actggccggc ggccggctgt ccttcgatcc
     1141 gctgcgggaa gtcgccgaca tggtggattc gaactgcgcc gacctgcact accgcgtcca
     1201 ggccgtggag cttgccgtcc aggccggcct cgacaagcaa ccggagccgg agcgcctgcc
     1261 cccgaacatc tacggcaccg aaggcgattg ggagacctat tccacgccct cgcgcgacgc
     1321 ccggctgaag accgccttca aggagctgcg cgacctcgcc gagcgcttca tgcgcatggc
     1381 cgacagccgc gacaaaaagc tcgcctatga cggaaaggat ctggccgccg acatgctcgc
     1441 cgtttatgac cgccgcgccg cgcagtgccg catcacttac gcgcgtagcg acggttcgcg
     1501 cttcaccatc ggctacgaag aggcgcgccg ccggctgttc gagatgtcgt tcgaccccta
     1561 ccagtgcgtc gagcggcgtt ggggggcgtc atcgggcgag gagttcgcgc cctgccccga
     1621 caccacgctg aagacggcct ggtacgaggc tgaacgcaac ctgcgcaacc agatcgaccg
     1681 cacctatgat gccaggatgg actttaccct cgcggagctg aaaaccccgg ggccggggaa
     1741 aggcgttccc gcgccgcccg ataccgacgt gcgggcctat ctgctgtcac gcttgcgccc
     1801 ggtggaaacg gcgtcggaaa agtgattccg gggcatcctt gatgtgtctt gatttcggcg
     1861 gaatcaacat atctcaccat gaccgggcgt ccgcacgctg gcgctacgga ccccggtcat
     1921 ccagaagctg gatgggcggc tcagggccgc ccatggtgag gggtgttaaa ttcaagtgca
     1981 gcaattcagc ccgaggacac tccagaactt ggttgccgca ttccgccctt gtcatggcag
     2041 gcttcgtcct gcaaggccgc gccccaaaga aaaaggcgcg acggagaccg ccgcgcccga
     2101 tttcttcgaa acagacgccg ctctagagat tgatccgatt taattgaatc ggatcaagct
     2161 ctagattcct tgtttttcgc gcaatctgac ggcaaaccgc gtcggcacgc tgctgctacc
     2221 gaccactttg ccggattgcg ctctagtgac cgcgcacgta cagcgaccac agacgggcga
     2281 tcatttcgcc gttcggcttg tttttcgctt tggcgaaagc cttcagcgcc gcgtcctttt
     2341 gcccggcgcc gtaaagcgct tggccgagac gcgtataagc gttgtcctcg tcgctcacgc
     2401 ctttgtcgat gccggctttc accgcgtcca cggcgtcctt gtaacggccc atgccgcaat
     2461 aattttcgcc cagtttgacc agcaggtcgc cggacttcgc cttgttcgcg gccgcgaccg
     2521 ttttcggcag gttggcgaga tcgctggcgg tgctggtctt ggccaggttc atcaggcggt
     2581 tggcgcggtc gccggacagc accttggcat cgaacccttt ctggatcacc gcgaccgctt
     2641 cgctcggaaa gccgagctgg atcgccaaag tggccagcag gaagtagtcg ctatcgccct
     2701 tcagcaaccc ggtcaaaagc ttgatgcggt tgatgtccag cgtctggtga tcggaaaggg
     2761 ccttggccgc ctccaccagc ttcaacagtt gggtccaatt ctggggcgtg ccgttgaccg
     2821 cgacaagctg ctccgccgca ctgcgcatca acgcatcgtc accgatcttg gcggcgcagg
     2881 tcatctgcca cttgagcatt tcctgtccgg cgctcgtatg ttcggaggca tagcgcgcgc
     2941 aacctttgaa gtcgcccagg cgataatacg cttgcgcgat gaacaccatg gtcgggccgt
     3001 ccagcgcgcc ggccttgcgc agcaagtcgg cgtcgttgat ggcgtcgcga tagcgcccgg
     3061 cgcgatagtc ggcctcgaac ttcgccttcg cgccgatcgc ggtgtcggca ccgcaggcgc
     3121 ctttcgatgc cgaggcgatg taattcttca tcgcggtgac gtagttctgt tcctcggccg
     3181 agaggccgcc gaccgcctgc gcttcccgga ccttcgccat ggcggcgttg cagttgccgg
     3241 cagccatggc ggccttcgcc gcattcaacg gattgccgac agcggcgcgg gccgcggcct
     3301 gcgccgaggt ggcggtgaag gcgctgccgg ccacaaccgt tacagccccc aacgacaatg
     3361 ccatagcgat ggcgtgcagt ttcttcggga tcatcagaga cctcgtcttt tttttacgtc
     3421 cggacgcagg cctcgcaagt acgcaggcgc acacccgcag accgctgcgt ccgctcccac
     3481 attactgcgt catatactgg tcgagaccgg taaacccgat cttcttgaca ccaagacgct
     3541 gggcgtccgc aagtacctta gcgacgatat cgtacctggc gagtttgttc ggattaaggt
     3601 ggatttcggg ctgaggatcc tgctgggatg cgttctggaa gtaggaagcc agcgttcccc
     3661 ggtcgaccgc cgttccattc cacagaatcg acccgtcgaa atccacgacc agattgacca
     3721 cgatcggctg aatctggggc ggattggggt tcggctgggg catgtccagc ttcacggcgt
     3781 gggtctggat cggcagggtg atgatgagca gagtgagcag aacgagcagc acgtcgatca
     3841 aaggcgtcat gttgatgtcc agcatgacat cgtcttcggc cttcattgcc attgccatgg
     3901 taatatacct tcctaattcg ctacgcgagt cactctatgt tgcgaggctc ggtgataaac
     3961 ccgaccttcg gaacgcccgc catctgacat gccagaatca cgcggccgag agcctggaac
     4021 ctcaccgacg cgtcgccccg gatatgcact tccggaagcg gcgtgccgct gtggaccgag
     4081 cggatcagcg cggcctcgaa acggccgcgc aaatccgaat actcgacggg tgcgttattc
     4141 cagtagatgt tctcgtttct atctaccgaa atcacgatat tttccggctt ggtgatcgtg
     4201 gcgatgttgc gtgcattggg cagcgccatc ggcacggtct ttatgatcac cggaatggtg
     4261 acgaggaaga tgatgagcat cacaagcatg atgtccacca acggaaccac gttgatggtg
     4321 gcgtttaact ccgcctcatc atgtcccgcg tcggcctgaa cgttcaaagc agccataact
     4381 attctccgcg aacctaactg tgatcagaac ggttctactt gcccttcgtg atcaggtagg
     4441 tctgcaggtc gcccgcgaaa ccgcgaagct tctcggtgat gaccttgttg cgacggacga
     4501 gccagttgta gagcagcact gccggaacgg ccgcggcaag accgagggcc gtcatgatca
     4561 gcgcctcgcc caccgggccc gcgaccttgt cgatcgaagc ctggccggcg acgccgatgg
     4621 cgatcagggc ttgcagaatg ccccaaaccg taccgaacaa gccgacgaag ggagccaccg
     4681 aaccgaccga tgcgaggaag gtaacgccgc cttgcagccg cgccgtggcg tcttcaagct
     4741 ggcgcgacag cgacatggcg atccagtcgt tgagactgac gaggctggtg ccgcccgcgg
     4801 ccgccttgac gccggcctcc gcgatggcgc ggaacatcga ggtcttcggc aacctgtcga
     4861 tgccctcgtt gatcgtgccc gaggtccaga agcgccgttc gacggtgcgc gcctggttga
     4921 ggatgcggct ctggtcgagg tacttggtga agaagatgta ccaagtgccg atcga
//
LOCUS       DASWPU010000010         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_36843,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000010 DASWPU010000000
VERSION     DASWPU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36843"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VGC27_00205"
     CDS             complement(<1..812)
                     /locus_tag="VGC27_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEY0281032.1"
                     /translation="MNLAQRPTQPCESLDAAAMPSVLEALHCLRVAITVFDAAERLIF
                     VNEHFNHLFRSLPPGDELIGLSYEELIRLELSGGEIADPGLPQDAGDFIAERRSQFTA
                     GQYHPRDLALAGGRIVEIKARRTASGGWIALWTDVTAARHVLGRLQNAIALSADAFAF
                     YDRNDRLLLCNDEYAAINGAERPEELTGRHFREVADRLVEHGRIVSAGDAGALLERRL
                     EAHRIVAGAMTVETASGTAYLLRDRATEDGGRVVVFTDVTEHRRVEKTLAEQT"
     gene            1040..2773
                     /locus_tag="VGC27_00210"
     CDS             1040..2773
                     /locus_tag="VGC27_00210"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY0281033.1"
                     /translation="MDIHGKSPAAKEANQTVQRQSFVAVQASKAAVGTVLSAAFVFLV
                     GRPDAAEAVAVAGLMAPALLALLAYTPVSLAFLEQAALMLFAALIGYLAVLTGGVISP
                     LVVWFALVPAEAALAGGRPAVLRAAFAAAVALLAVAFIQASGALPVSRLPSPAWMFYA
                     VSVFAAVIQAAFVAAASQDRQRAADRAAAEGAAMYRFLADNAMDMITRHSDDGRIRFA
                     SPAARALFGRPPESLLGLAPSALVHPDDLKAIQAAFMEASYFARPASAEVRLKRADGS
                     HVWAEIRCRPVPKQNGEASDIVAITRDITERKAYERELIEARDLSEQASRAKSRFLAN
                     MSHELRTPLNAIIGFSEVMAHEMFGALGGPRYLEYSRLIHESGNHLLELINGILDMSK
                     IEAGKFELSEEVFDLAEVAEASARFVKLPAERAGVAIKTAIAPGAKQIFADKRAVKQI
                     LVNLLSNGVKFTPRGGEVRIRAALDAGGIEIAVADTGVGISKKDLDRLGKPFEQVEGE
                     HVRAKEGTGLGLSLVKALAMMHGGEAVLESTLGEGTVVRVRLPHAAVDDKGKRVVSQE
                     TNILPFRGAAA"
     gene            complement(2935..>3678)
                     /locus_tag="VGC27_00215"
     CDS             complement(2935..>3678)
                     /locus_tag="VGC27_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY0281034.1"
                     /translation="IAVNHLVWGRKLRASAADRARVMALDASREIKDKRLLTYSVWVI
                     GLLIAGFALGEMLHLRAATIALFGGATMLLLDNFRHHRHKHNENILAILNDVEWITIF
                     FFVGLFVVVGGVEKAGLLSYLAGKLTAATGGDMKLTALGILWASAGASAIIDNIPFVA
                     TMIPLLKTMAPGLGGVTQMDPVWWSLALGACLGGNGTLIGASANLVVAGQAEKQGVRF
                     SFVKFALLAFPMMLMSIAICHFYILWRYF"
BASE COUNT          627 a         1186 c         1235 g          630 t
ORIGIN      
        1 gtctgctcgg ccagggtttt ctcgacgcgg cggtgctcgg tgacgtcggt gaagacgacg
       61 acgcgcccgc cgtcttcggt ggcgcggtcg cgcaggagat aggccgtgcc ggatgcggtt
      121 tccacggtca tggcgccggc cacgatgcga tgcgcttcga ggcgccgctc cagcaaggcg
      181 ccggcgtccc cggcgctgac gatccgcccg tgttcgacca gcctgtccgc cacctcgcgg
      241 aagtgccggc cggtcagctc ttcgggccgc tccgcgccgt tgatcgcggc gtactcgtcg
      301 ttgcacagca gcagcctgtc gttgcggtcg tagaaggcga aggcatcggc cgacagcgcg
      361 atggcgtttt gcaggcggcc gagaacatgg cgcgccgcgg tcacgtcggt ccacagggcg
      421 atccagccgc cgcttgccgt acgccgcgcc ttgatctcga cgatgcgtcc gccggcgagc
      481 gcgaggtcgc gcggatgata ttggcccgcg gtgaattgcg aacggcgctc cgcgatgaaa
      541 tcgccggcgt cttggggcag accgggatcg gcgatctcgc cgccgctcag ttcgaggcgg
      601 atgagttctt cgtagctcag gccgatcagc tcgtcgcccg gcggcaggga acggaacagg
      661 tggttgaaat gctcgttgac gaagatcagc cgctcggcgg catcgaagac ggtgatggcg
      721 acgcgcaggc aatgcagcgc ctccaggacg gacggcatcg ccgccgcgtc gaggctttcg
      781 catggttgcg tcggccgctg cgccagattc aaggaaaatt cccttccccc ttgccgaagc
      841 gactttgccg gtaacgggtt gcggaaaggt taggccgtgg ccgggcgcgc ccgaatcacg
      901 ccacctttgt taccaaagcg taaacgcagg aggctgcaaa tcttgtgaat ttgcaaacaa
      961 atcggcggcg cgttcaaagt tcgttaagca ggcgaacgcc aggatgcgcc cgcgcgagac
     1021 cacaagacgg tcggggttaa tggatattca cggaaaatcg ccggcggcca aggaagcgaa
     1081 tcagacggtg cagcgccagt cgttcgtcgc ggtccaggcg tctaaggcgg cggtcggcac
     1141 tgttctctcg gccgccttcg tctttcttgt cggccgcccg gatgccgccg aagccgtcgc
     1201 cgtcgccggg ctgatggcgc cggcgctgtt ggccttgctg gcctatacgc ccgtttcgct
     1261 cgcgtttctg gaacaggccg cgctgatgct tttcgccgcc ttgatcggct acctggccgt
     1321 gctgaccggc ggcgtgattt cgccgctggt ggtgtggttc gcactggtgc ctgccgaagc
     1381 ggcgctggcc ggagggcgtc ccgcggtgtt gcgcgccgcg ttcgccgcgg ccgtggcgct
     1441 tttggccgtg gccttcattc aagcgtccgg cgcgctgccg gtttcgcgcc tgccgtcgcc
     1501 ggcctggatg ttctacgccg tttcggtgtt cgccgcggtg attcaggctg ccttcgtcgc
     1561 cgccgcgtcg caggaccgcc agcgcgccgc cgacagggcc gccgccgaag gcgccgccat
     1621 gtaccgcttc ctcgccgaca acgcgatgga catgatcaca aggcacagcg acgacggacg
     1681 catccgcttc gccagtcccg ccgcgcgcgc gctgttcggc cggccgcccg aatctttgct
     1741 cggcctggcg ccgtcggcgc tggtccatcc cgacgatttg aaggcgatcc aggcggcgtt
     1801 catggaggcg agttatttcg cgcggccggc ctcggcggaa gtccgcctca agcgcgccga
     1861 cggcagccat gtgtgggccg aaatccgctg ccgccccgtg cccaaacaga acggcgaagc
     1921 ctccgacatc gtcgccatca cccgcgacat caccgaacgc aaggcctatg aacgcgagct
     1981 gatcgaggcg cgcgatcttt ccgaacaggc cagccgcgcc aaatcgcgtt ttctcgccaa
     2041 catgagccac gaactgcgca ccccgctcaa cgccatcatc ggcttttccg aagtgatggc
     2101 gcacgaaatg ttcggcgcgc tcggcgggcc gcgctatctc gaatattcgc ggctgatcca
     2161 cgaatcggga aaccatctcc tggagctgat caacggcatc ctcgacatgt cgaagatcga
     2221 ggcgggcaag ttcgaacttt cggaagaagt gttcgatctg gccgaagtgg cggaggcctc
     2281 ggcgcgcttc gtcaagctgc cggccgagcg ggcgggcgtg gcgatcaaga ccgcgatcgc
     2341 gccgggcgcc aagcagatct tcgccgataa gcgggcggtc aagcagattc tcgtcaacct
     2401 attgtcgaac ggggtgaagt tcaccccgcg cggcggcgag gtacgcatcc gcgccgcgct
     2461 ggatgcgggc ggcatcgaaa tcgcggtcgc cgataccggc gtcggcattt ccaagaagga
     2521 cctcgaccgg ctgggtaagc cgttcgaaca ggtcgaaggc gagcatgtgc gcgccaagga
     2581 aggcaccggg cttggcctgt cgctggtcaa ggcgctggcg atgatgcatg gcggcgaagc
     2641 ggtgctggaa tccacgctgg gcgaaggcac cgtggtgcgc gtgcgcctgc cgcacgccgc
     2701 ggtggacgac aagggcaagc gcgtggtgtc ccaggaaacg aatatcctgc ccttccgcgg
     2761 cgcggccgcg tagagcgtga tacacactgt tggaatcgta cgaccatcat gtttcgccgg
     2821 gcgcctgctt cgacaggctc atgcttcgac aggctcagca tgaaggtata gccatacccc
     2881 tcaccctgag cttgtcgaag ggtgagcttg tcgaagggtg gaacgtacga cagttcagaa
     2941 atagcgccat agaatataga aatggcagat ggcgatgctc atcagcatca tcgggaacgc
     3001 gagcagcgcg aacttcacga agctgaagcg cacgccttgc ttttccgcct gacccgccac
     3061 caccagattg gcgctggccc cgatcaaggt gccgttgccg cccaggcacg cccccagcgc
     3121 cagcgaccac cacaccgggt ccatctgcgt gacgccgccg aggccgggcg ccatggtctt
     3181 gagcagcgga atcatggtgg cgacgaaggg gatattgtcg atgatcgccg aggctccggc
     3241 cgacgcccac aggattccga gcgcggtgag cttcatatcc ccgccggtcg ccgccgtcag
     3301 tttgccggcg agataggaga gcagccccgc cttctcgacg ccgccgacca ccacgaacag
     3361 gccgacgaag aagaagatgg tgatccattc gacgtcgttg aggatcgcca ggatgttctc
     3421 gttgtgcttg tggcgatgat ggcggaaatt gtcgagcagg agcatcgtgg cgccgccgaa
     3481 caaggcgatg gtcgccgccc gcaagtggag catctcgccc agcgcgaaac cggcgatgag
     3541 caggccgatc acccacaccg aataggtgag cagccgctta tccttgatct cgcggcttgc
     3601 gtcgagcgcc atgacgcggg cgcggtccgc ggccgaggcg cgcagcttgc ggccccagac
     3661 caggtgattg acggcgat
//
LOCUS       DASWPU010000011         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_79574,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000011 DASWPU010000000
VERSION     DASWPU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79574"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..1424
                     /locus_tag="VGC27_00220"
     CDS             357..1424
                     /locus_tag="VGC27_00220"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0281035.1"
                     /translation="MKNVMLVGGGKIGVAIAQFLSETGDYHVTVVDRDAASLARMPKN
                     VELRKVAIDAPGAFAGEIEGNDVVLSATPYHLTATIAEAAKRAGAHYLDLTDDVESTR
                     SIKKLAEGAKTAFIPQCGLAPGFISIAAYDLAKKFDRLRDVNMRVGALPEFPTNALKY
                     NLTWSTDGLINEYCNPCESIRDGVRGEVPALEETELFSLDGTEYEAFNTSGGLGTLCD
                     TLEGKVENLNYKTVRYPGHRDIVKMLVRDLRLGVRRDILKDVLETAIPITYQDVVLIF
                     VTVSGWQGGLLTQESYAKKIYAQTIGDRLMSAIQVTTAAGICATCDLLVDGKLPQKGF
                     VRQEEIGLADFLSNRFGKYYA"
     gene            1516..3054
                     /locus_tag="VGC27_00225"
     CDS             1516..3054
                     /locus_tag="VGC27_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0281036.1"
                     /translation="MHADLLSIFGIASSGPQIVHSPIDGSEIGRLAYDEAPTIEAKIA
                     ASVEAFRHWREVPAPRRGELVRLFGEELRVHKEALGRLVTVENGKILQEGLGEVQEMI
                     DICDFAVGLSRQLYGLTIASERPGHRMMETWHPIGPVAVISAFNFPVAVWAWNAALAL
                     VCGDSMLWKPSKKTPFTALATQALFARAAKRFGDAPAGLAAPAGLAAPAGLAAPAALS
                     QIVLATREAGDALTKDPRIPVVSATGSTAMGRVLGPAVAMRFGRSILELGGNNGMIVC
                     PSAKLELAERAILFAAVGTAGQRCTSLRRLFVHNEIYDRLVGALKSHYAKLVIGDPLA
                     KTTLVGPLIDRAAFEAMEHALAEAAREGGKITGGGRVLADEYPEAYYARPAIAEMPKQ
                     TPIVMRETFAPILYVMRYRDFAEVLGEHNAVPQGLSSCIFTRDLQEAERFLAASGSDC
                     GIANVNIGPSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTATINYSDALPLAQGI
                     EFGG"
BASE COUNT          545 a          980 c          988 g          562 t
ORIGIN      
        1 ttcaccgtat agcccttgat ggtgccttcg cgttccagcc gcgcgatgcg ctcctgcacg
       61 gtggagcgcg ccagtcccag cttgcgcgcc agcgaggcgg tgggctcgcg cgcattggcg
      121 cgcaacaagg cgatcaattt ggcgtcacct gggcgcattc gctttcacct ttcgacaaac
      181 tcggagtgaa gacatcccct catgctgagc ctgtcgaagc atgagggccg gtccatcatt
      241 ttgccggagt tcccggcgat tcgccggatc atagcgcaag ttccggcgca ttgccgcctg
      301 ctaaccggcg cggtttggcg gcacatttca cccgtagcgc aatgagagtg cggcccatga
      361 aaaacgtgat gctcgtcggc ggcggcaaga tcggcgtcgc catcgcccaa ttcctctcgg
      421 agaccgggga ttaccatgtg acggtcgtgg accgcgacgc cgcctcgctg gcgcgcatgc
      481 ccaagaatgt cgagctgcgc aaggtcgcca tcgacgctcc cggcgccttc gcgggcgaga
      541 tcgagggcaa cgatgtcgtg ctttcggcca cgccctatca tctgaccgcc accatcgccg
      601 aagcggccaa gcgcgccggc gctcattatc tcgacctcac cgacgatgta gaatccaccc
      661 gcagcatcaa gaagctggcc gagggcgcca agaccgcctt cattccgcaa tgcgggctgg
      721 ccccgggctt catctccatc gccgcctacg atctggccaa gaagttcgac aggctgcgcg
      781 acgtcaacat gcgggtgggc gcgctgccgg aattccccac caatgcgctg aaatacaatc
      841 tcacctggtc caccgacggg ctgatcaacg aatattgcaa cccgtgcgaa tccatccgcg
      901 acggcgtgcg cggcgaagtg ccggcgctgg aggagacgga gcttttctcc ctcgacggca
      961 ccgaatacga ggccttcaat acctccggcg ggctgggcac gctgtgcgac acgctggaag
     1021 gcaaggtcga aaacctcaat tacaagaccg tgcgctatcc cggccatcgc gacatcgtaa
     1081 aaatgctggt gcgcgatctg cgcctgggcg tgcgccgcga catcctcaag gacgtgctgg
     1141 agactgcgat ccctatcacc tatcaggacg tagtgctgat cttcgtcacg gtctcgggct
     1201 ggcagggcgg cctactcacg caggaaagct acgccaagaa aatttatgcg cagaccatcg
     1261 gcgacaggct gatgagcgcc atccaggtga cgaccgcggc gggcatctgc gccacctgcg
     1321 acctcctggt ggacggaaag ctgccgcaga agggctttgt gcgccaagag gaaatcggcc
     1381 tggcggattt cctgtccaac cgcttcggga agtattacgc gtagtggcga atagggggta
     1441 gcgaatagcg aatagggttc gctgtttccc tattcgctac tcgctattcg ccattcgctt
     1501 tccttggaga taaccatgca cgccgatctg ctctctatct tcggcatcgc ctcgtcgggt
     1561 ccccaaatcg tgcattcgcc catcgacggt tccgagatcg gccgcctcgc ctatgacgag
     1621 gcgccgacga tcgaagcgaa aatcgcggcg agcgtggaag ccttccgcca ctggcgcgag
     1681 gtgccggcgc ccaggcgcgg cgagctggtg cgcctgttcg gcgaggagct gcgcgtccac
     1741 aaggaagcgc tcggccgtct ggtcaccgtc gagaacggca agattctgca agagggtctc
     1801 ggtgaagtgc aggagatgat cgacatctgc gatttcgcgg tcggtctgtc gcgccagctt
     1861 tacggcctca ccatcgccag cgagcggccc ggccatcgca tgatggaaac ctggcatccc
     1921 atcggcccgg tggcggtgat ctcggcgttc aattttccgg tggcggtgtg ggcgtggaac
     1981 gccgcgctgg ctttggtctg cggcgacagc atgctgtgga agcccagcaa gaagacgccg
     2041 ttcaccgcgc tcgccaccca ggccttgttc gcccgtgcgg cgaaacgatt cggcgatgcg
     2101 cccgctgggc tcgcagcccc cgctgggctc gcagcccccg cggggctcgc agcccccgct
     2161 gctctctcgc aaattgtgct agcgacgcgc gaggccgggg acgctttgac caaggatccg
     2221 cgtattccgg tggtcagcgc cacgggatcg acggcgatgg gccgcgtgct cggtccggcg
     2281 gtggcgatgc gcttcgggcg gagcatcctg gaactgggcg gcaacaatgg gatgatcgtt
     2341 tgtccctcgg ctaagctgga gctggcggaa cgcgccatcc tgtttgccgc ggtgggcacc
     2401 gccggccagc gctgcacctc gctgcgccgc ctgttcgtgc ataacgaaat ttacgacagg
     2461 ctggtggggg cgctgaaatc gcattacgcc aaactcgtta tcggcgatcc gctggcgaag
     2521 acgacgctgg tggggccgct gatcgaccgc gccgctttcg aggcgatgga gcatgcgctg
     2581 gccgaggccg cgcgcgaagg cggcaagatc acgggcggcg ggcgcgtgct ggcggacgaa
     2641 tatccggagg cttattacgc gcgtcccgcc atcgcggaaa tgccgaaaca aacgcctatc
     2701 gtcatgcggg aaaccttcgc accgatcctc tatgtcatgc gctatcgcga tttcgccgag
     2761 gttctgggcg agcataatgc tgtgccgcag gggctttcgt cctgcatctt cacccgcgac
     2821 ttgcaagagg cggagaggtt cctcgcggcg tcgggatcgg attgcggcat cgccaatgtg
     2881 aacatcggcc cttccggcgc ggagatcggc ggcgccttcg gcggcgaaaa ggaaaccggc
     2941 ggcggacgcg agtccggttc ggacgcctgg aaggcctata tgcgccgcca gaccgcgacc
     3001 atcaattatt ccgatgcgct gcctttggcg caggggatcg agttcggcgg ttagcggccc
     3061 tcgttaacct ctctt
//
LOCUS       DASWPU010000012         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_6281, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000012 DASWPU010000000
VERSION     DASWPU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6281"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VGC27_00230"
     CDS             <1..731
                     /locus_tag="VGC27_00230"
                     /inference="COORDINATES: protein motif:HMM:NF016439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEY0281037.1"
                     /translation="MIAEIRDLSPKPGLSLGSETVQAVVPDVIVREGPDGGWLVELNS
                     DTLPRLLVNARYFARISAHARDKDSKTYLTECLNNANWLVKSLDQRARTILKVASEIV
                     RQQDGFLTHGVRHLRPLNLRTIADAIGMHESTVSRVTSNKYVATPRGLFELKYFFTAA
                     IQSMNGAESHSAEAVRDRIREMIEHEGGSDVLSDDRIVSLLTADGVSIARRTVAKYRE
                     AMHIPSSVERRRLKGGETAASLHS"
     gene            934..1407
                     /gene="ptsN"
                     /locus_tag="VGC27_00235"
     CDS             934..1407
                     /gene="ptsN"
                     /locus_tag="VGC27_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEY0281038.1"
                     /translation="MQIADLLSPEGVFAALKVQGKKQLFLELSARAASLVQVPERRVF
                     ETLIERERLGTTGVGQGIAIPHGRLVNLEKIVGVFVRLETPIDYDAVDKQPIDLVFML
                     LAPEGAGADHLKALARVSRLLRNQAVCEKLRAANSAEALYAILTEPAAGANSVAA"
BASE COUNT          386 a          566 c          604 g          365 t
ORIGIN      
        1 acatgatcgc ggagatccgc gacctctcgc cgaagcctgg cctgtcgtta ggcagcgaga
       61 cggtgcaggc cgtggtgccg gacgtgatcg tgcgcgaagg gcccgacggc ggctggctgg
      121 tggaactgaa ttcggacacg ctgccgcggc tgctggtcaa tgcgcgctac ttcgccagaa
      181 tctcggcgca cgcccgcgac aaggattcca agacctatct caccgagtgc ctgaacaacg
      241 ccaactggct ggtgaagagc ctggaccagc gcgcccgcac gatcctgaag gtcgccagcg
      301 aaatcgtgcg ccagcaggac ggctttttga cccacggcgt gcgccatctg aggccgctca
      361 acctgcgcac catcgccgac gccatcggca tgcacgaatc gacggtcagc cgcgtcacct
      421 cgaacaaata cgtcgccacg ccgcgcggcc tgttcgagct gaaatatttc ttcaccgccg
      481 ccatccagtc gatgaacggc gcggaatcgc attccgccga agcggtgcgc gaccgcatcc
      541 gcgagatgat cgagcacgaa ggcggcagcg acgttctctc cgacgatcgc atcgtttcgc
      601 tgctgacggc ggacggcgtg agcatcgcca gacgcacggt cgcaaagtat cgcgaggcca
      661 tgcacatccc gtcttccgtg gaaaggcgca gactcaaggg cggagaaacc gcggcttccc
      721 tgcattcgtg aatttcaagc gttgactttg gaaaaagtga ggactaaacg tccgcgcccc
      781 ttgaacgagg ccccgtaatg cggcgctttt gttacacaaa aggcaatgcg gtgggcggcg
      841 gaatgaatgc aaggcgggct gcggctcgcg cggttaaagt ttgaacgggc gtcgggcctg
      901 attctcagcc aaaaccttag caaaagggcc gtcatgcaga ttgcagattt gcttagcccg
      961 gaaggcgttt ttgcggcgtt gaaggtccaa ggcaagaagc agctttttct ggaattgtcg
     1021 gcgcgggcgg cgtccctggt gcaggttccg gagcggcggg tttttgaaac cttgatcgag
     1081 cgcgagcgtc tggggacgac cggcgtgggg cagggcatcg ctattccaca cggtcgcctc
     1141 gtcaatctcg aaaagatcgt cggcgtcttc gtgcggctgg agacgccgat cgattacgac
     1201 gcggtggaca agcaacccat cgatctggtg ttcatgctgc tggcgccgga aggcgccggc
     1261 gcggatcatc tgaaggcgct ggcgcgcgtt tcgcggctgc tgcgcaatca ggccgtctgc
     1321 gagaaactgc gcgcggcgaa ctccgccgag gcgctatacg ccatcctcac cgagccggcc
     1381 gccggcgcga attcggtcgc ggcgtaaaac tttgtggctg tcttggagcg ggttgagacc
     1441 aggtgggatc ataaactcac ttgtcatggc ccgcgaatgc gggccaccca gttgaaaccg
     1501 gctcaactat tcgaggaaag tacggtgaaa tttctttggc ggtgtgacga tgtcatctgg
     1561 gggacccgca caaagtttac acttcggctg cgcttcgcgc gaaccggggt ggcgggccgt
     1621 gacgccagga gttcttaggt cgccttgatg tcagatcgcc ttagagcgca atccggcaaa
     1681 gtggtcggta gcagcagcgt gccgacgcgg tttgccgtca gattgcgcga aaatcaagga
     1741 atctagagct tgatccgatt caatcaaatc ggatcaagct ctagtgaacc cgatttcttt
     1801 gcctcctccc ttgaggggag tgaagcgtcg ttgaactccc gtaaagaacc cttatatcca
     1861 tcgatcatgg acaaaaccga ctacacccgc gcccccgcgg ccgatttcgt gctgccgttc
     1921 g
//
LOCUS       DASWPU010000013         5711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_79582,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000013 DASWPU010000000
VERSION     DASWPU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79582"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGC27_00240"
     CDS             complement(<1..218)
                     /locus_tag="VGC27_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281039.1"
                     /translation="MKLGAIAALLVGLAIAIFVLLHIGIAPVLDAVASVSWGGFVLIC
                     LFGLLPPMLSGLAVYALLPGQAPWLVFLA"
     gene            complement(259..1236)
                     /gene="hpnK"
                     /locus_tag="VGC27_00245"
     CDS             complement(259..1236)
                     /gene="hpnK"
                     /locus_tag="VGC27_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HEY0281040.1"
                     /translation="MTARTEKVGTGFSMSPRGDKKQRTEKVGTGFSMSPRGEKKRLIV
                     TADDFGAAVQVNDAVERAHREGILSAASLMVSAPAAADAVKRAKALPGLGVGLHLVLV
                     EGRPALAAADIPDLVDEDGMFRNDMVRASFAMFLRPKVRRQLAAEIEAQFEAFAATGL
                     ALDHVNAHKHFHLHPTIAATILRVGRRFGMKAMRVPYEPAALLRRIEPGAQGDFVVEL
                     WARRLRRRLAAAGILSPERTFGLCWSGAMTAARLAALLEHLPEGLSEIYLHPAAGPGY
                     PGSTPTYLYEQELAALTSSQVVDITKKSKITLGSFAGFLPRGEGAKVGR"
     gene            complement(1233..2657)
                     /gene="hpnJ"
                     /locus_tag="VGC27_00250"
     CDS             complement(1233..2657)
                     /gene="hpnJ"
                     /locus_tag="VGC27_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HEY0281041.1"
                     /translation="MKSLFLQAPSFDGYDGGAGARYQMKREVKSFWYPTWLAQAAAMV
                     EGSKLIDAPPHGLKFADIVDDVKAHELIVIHTSTPSFKSDCKTARMIKELNPSAVIGF
                     IGAKVAVQPGESLEACEAIDFVARNEYEFTIKEIAEGGDWAAIKGMSYRGRDGAIVHN
                     EEREILMDMDKLPSVLPLYKQLDTRKYFGGYLKHPYISFYTGRGCKSRCTFCLWPQTI
                     SGHKYRFRSIPVVVEEIKYIQKNFPEVKEIFFDDDTLTDDHDRVEALAVELGKLGVVW
                     SCNAKANVPRKTLEVLKANGLRLLLVGYESGNQKILHNIKKGLLVDVARKFAKDCREL
                     GIIVHGTFILGLPGETRETIRETLAFAQEVNPRTLQVSLAAPYPGTFLYNQAKENGWF
                     ASDVDLLTEDGTQIAPLNYPDLPHGEIFASVEDFYKKFYFRPSKIAEIVGEMLTSRDM
                     LVRRLREGMEFFQFLRTREDMIVS"
     gene            complement(2668..3807)
                     /gene="hpnI"
                     /locus_tag="VGC27_00255"
     CDS             complement(2668..3807)
                     /gene="hpnI"
                     /locus_tag="VGC27_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HEY0281042.1"
                     /translation="MIFFTVLGWLSAALALGGAIYTVLAAIAAGRYAPRSEPAAASPP
                     AATVLKPLHGAEPNLEDNLATFFVQDYPAPFQLVFGVQDEADPAIQVVERLRARYPGV
                     NAALVVDGRRHGSNAKISNLINMQEAARHDVLILSDSDIAVPSDYLGRVATALEPDEV
                     GAVTCLYTGWAGAGVGSRLCAMGIDYQFLPNAVTSIVLGLAEPCFGSTIALKRKVLDE
                     IGGLRAFANFLADDYEIGRAVRARGYHIAFPRLVVRHTCNEKSFGEWLAHELRWARTL
                     RIVTPAGHMSSIVTHAIPLALLGVILTGFTLFALASLVTTVGARAMLKWRIDRLYRCN
                     GGSIWLLPARDVLSFGVFLVSLFGGSVEWQGERLRVKQDGALSEW"
     gene            complement(3885..4991)
                     /locus_tag="VGC27_00260"
     CDS             complement(3885..4991)
                     /locus_tag="VGC27_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY0281043.1"
                     /translation="MADDILKIRPAGECKWDCVSFGEVMLRFDPGFGRVRNARQFQVW
                     EGGGEYNVARAMRKCWGKRSSVVTALPVNDLGWLVEDFICQGGVDTSHIVWREFDGLG
                     RNTSVGLNFTEKGYGIRPALGCSDRGNSAASQIKPGEVDWDKLFGEEGVRWFHTGGIF
                     AALAPNTSEAVIEAVQAAHKYGTVVSYDLNYRASLWKSQGGKDGARKINREIAKYVDV
                     MLGNEEDFTACLGFEVEGLDEHISKIDPANFKKMIAKAVQEYPNFKVAATTLRNAKTA
                     TFNDWGAVIFAGGSFYEAPMRENLEIYDRVGGGDGFASGLAYGFLEGKGPQAAVEYGA
                     AHGALAMTTPGDTSMVNVKEVEAAMKGKGARVIR"
     gene            complement(4994..>5711)
                     /locus_tag="VGC27_00265"
     CDS             complement(4994..>5711)
                     /locus_tag="VGC27_00265"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY0281044.1"
                     /translation="GNRFHLPKLQLDCQGMAKDRMTVLAAMMEQGVIPVFYHPDVEVC
                     TNVIQACADGGAKCIEFTNRGDFAPHVFLEVTRHFAKADKSVIMGVGSVLDAPTAGIY
                     IANGANFVVGPVLNADVAKVCNRRKIPYSPGCGSASEISYAEELGVEIVKIFPGTQVG
                     GPEFVKAVLGPMPWTKIMPTGGVDPTEESLKKWFGAGIVAAGIGSKLITSELLKAKDY
                     KGIETKVAETIALIKKIRGK"
BASE COUNT         1038 a         1817 c         1800 g         1056 t
ORIGIN      
        1 gccaggaaga ccagccatgg ggcttgcccg ggcagcaatg cgtagacggc cagccccgac
       61 aacatgggcg ggaggagtcc gaaaaggcag atcagcacga agccgcccca gctcacgctg
      121 gcaacggcat ccagcaccgg cgcgatgccg atatgcagga gcacgaaaat cgcaatcgcc
      181 agtccgacga gcagagcagc gatagcgccg agtttcatgt gcatttgaat ggtcgcggat
      241 gggatggtgg aaggcgattt acctgccgac tttagcgcct tcgccgcgcg gcaggaaacc
      301 ggcgaatgat cccagggtga ttttgctttt cttcgtaatg tcaacgactt ggcttgacgt
      361 caaggccgcc agttcctgct cgtagagata ggttggcgtg gaccccggat aacccggccc
      421 ggccgccgga tgcaggtaga tctcgctgag gccttcgggc agatgctcca gcagagcggc
      481 cagccgcgcc gccgtcatgg cgcccgacca gcacaacccg aacgtgcgtt caggggaaag
      541 gatgccggcg gccgcaagcc ggcggcgcag acggcgggcc caaagctcga cgacgaaatc
      601 tccctgcgcc cccggctcga tccggcggag aagcgcggcc ggctcgtaag gcacgcgcat
      661 ggctttcatg ccgaaacggc ggccgacgcg caggatcgtc gcggcaatcg tcgggtgcag
      721 atggaaatgt ttgtgcgcgt tgacgtgatc gagtgcgagg ccggtcgccg cgaaggcctc
      781 gaactgggct tcgatctcgg cggccagttg gcggcggacc ttcggccgca ggaacatggc
      841 gaagctcgcg cgcaccatgt cgttgcggaa catgccgtcc tcgtccacga gatcgggaat
      901 gtcggctgcg gcaagcgccg gcctgccctc caccagcacc agatgcaggc ccacgcccag
      961 gcccggcaac gcttttgcgc gcttgaccgc gtcggcggcg gcgggcgccg aaaccatcag
     1021 gctggcggcg ctgagaatcc cctcgcgatg ggcgcgctcg accgcgtcgt tgacctggac
     1081 ggcggcgccg aaatcgtccg cggtgacgat caatcgtttt ttttcgccgc gcggactcat
     1141 cgaaaaaccg gtgcccactt tttcggtccg ctgtttttta tcgccgcgcg gactcatcga
     1201 aaaaccggtg cccacttttt cggtccgcgc ggtcaagaaa cgatcatgtc ctcgcgcgtg
     1261 cgcaggaact ggaagaactc catgccctcg cgcagacgcc gcaccagcat atcgcgcgag
     1321 gtcagcatct cgccgacgat ttcggcgatt ttcgacggcc ggaaatagaa cttcttatag
     1381 aaatcctcca ccgaagcgaa aatctcgccg tggggcaggt ccggataatt gagcggcgcg
     1441 atctgggtgc cgtcctcggt caatagatcg acgtcggaag cgaaccagcc gttttccttc
     1501 gcctgattgt agaggaaggt gccgggatag ggcgcggcca gcgatacttg cagcgtgcgc
     1561 gggttgactt cctgggcgaa ggccagcgtt tcccggatgg tctcgcgggt ctcgcccggc
     1621 aggccgagga tgaaggtgcc gtggacgatg atgccgagct cgcggcagtc cttggcgaac
     1681 ttgcgcgcca cgtccaccaa gaggcccttc ttgatgttgt gcaggatttt ctggttgccg
     1741 gattcgtagc cgaccagcag caggcgcagc ccgttcgcct tcagcacttc cagcgtcttg
     1801 cgcggcacgt tggccttggc gttgcacgac catacgacgc cgagcttgcc caattccacc
     1861 gccagcgctt cgacgcggtc gtgatcgtcg gtcagggtgt cgtcgtcgaa gaagatttcc
     1921 ttcacctcgg ggaaattctt ctggatgtac ttgatctcct cgaccacgac cgggatcgag
     1981 cggaagcggt atttgtgccc gctgatggtc tgcggccaca ggcagaaggt gcagcgcgat
     2041 ttgcagccgc ggcccgtata gaacgatatg taggggtgct tgagatagcc gccgaaatat
     2101 ttccgggtat cgagctgctt gtaaagcggc agcaccgacg gcagcttgtc catgtccatg
     2161 aggatttcgc gttcctcgtt atggacgatc gcgccgtccc tgccccggta ggacattccc
     2221 ttgatcgccg cccagtcgcc gccttccgcg atttccttga tggtgaattc gtattcgttg
     2281 cgggcgacga aatcgatcgc ctcgcaggct tccaggcttt cgcccggctg caccgccacc
     2341 ttggcgccga tgaacccgat cacggcggag ggattcagct ccttgatcat ccgcgcggtc
     2401 ttgcagtcgg atttgaacga cggcgtggag gtatgaatga cgatcagctc gtgcgccttc
     2461 acgtcgtcga cgatatccgc gaatttcaat ccgtgcggcg gggcgtcgat cagcttggag
     2521 ccttccacca tcgcggcggc ctgggccagc caagtcggat accagaacga cttgacctcg
     2581 cgcttcatct gatagcgggc gccggctccg ccgtcgtagc cgtcgaacga aggcgcttgc
     2641 aggaacaagg acttcattac tcgaacctca ccattccgac aacgcgccgt cctgcttcac
     2701 ccgcaggcgt tcgccttgcc attccaccga tccgccgaac agcgaaacca gaaagacgcc
     2761 gaacgatagc acgtctctgg cgggcagcag ccaaatcgat ccgccgttgc aacgataaag
     2821 ccggtcgatg cgccatttga gcatggcccg cgcgcccacg gtagtaacaa ggctcgccag
     2881 cgcaaacaag gtaaagccgg tcaggattac ccctagcaac gctaagggaa tggcatgggt
     2941 cacgatgctg gacatatggc cggcaggcgt aacgatgcgc agggtgcgcg cccagcgcaa
     3001 ctcgtgggcc agccattcgc cgaaggattt ctcgttgcag gtatggcgca ccacgaggcg
     3061 cgggaaggcg atgtgatagc ccctggcccg cacggcgcgg ccgatttcgt aatcgtcggc
     3121 caggaagttg gcgaacgccc gaagcccgcc gatctcgtca agcaccttgc gcttgagcgc
     3181 gatggtcgaa ccgaagcaag gctcggcaag gcccagcacg atgctggtca cggcgttggg
     3241 caggaactgg tagtcgatcc ccatggcgca gaggcgcgag ccgacgcctg cgcccgccca
     3301 gcctgtatac aggcaggtga ccgcgcccac ctcgtccggc tccagcgccg tcgccacccg
     3361 gccgagatag tcggaaggca cggcgatgtc gctgtcgctc aggatcagca cgtcgtgacg
     3421 cgccgcttcc tgcatgttga tcaggttcga gattttggcg ttggagccgt gccgccgccc
     3481 gtccaccacc agcgccgcgt tcacgccggg gtagcgggcg cgcaggcgct cgaccacttg
     3541 gatggccgga tcggcttcgt cctgcacgcc gaagacgagc tggaacgggg ccggataatc
     3601 ctggacgaag aaggtggcga gattgtcttc gagattcggc tcggcgccgt gcagcggctt
     3661 gagaaccgtc gcggccggcg gcgacgctgc ggcgggctcg cttctggggg cgtaacggcc
     3721 ggcggcgatc gccgccagaa cggtgtaaat ggcgccgccc agcgcaagcg ccgccgacag
     3781 ccagcccagg acggtgaaga atatcattcc gctgcgctcc gcgccgctac ccttgcgcgg
     3841 atgcgatcat tacacgtttc aagcgaactt tgaacgcccg ccgcttaccg gatgacccgc
     3901 gcgcccttgc ccttcatcgc ggcttcgacc tccttgacgt tgaccatgga ggtgtcgccg
     3961 ggcgtggtca tggccaaagc gccgtgcgcg gccccgtatt cgaccgccgc ctgaggtccc
     4021 ttgccctcaa ggaagccata ggccaggccg gaggcgaaac cgtcgccgcc gcccacgcgg
     4081 tcgtagattt ccagattctc gcgcatcggc gcttcgtaga aactgccgcc agcgaagatc
     4141 accgcgcccc aatcgttgaa ggtggcggtc ttggcgttgc gcagcgtggt cgccgccacc
     4201 ttgaagttgg gatactcctg caccgccttg gcgatcatct tcttgaagtt cgccggatcg
     4261 atcttggaga tgtgttcgtc gagaccttcc acctcgaagc cgaggcaagc ggtgaagtct
     4321 tcctcgttgc cgagcatgac atcgacgtac ttggcgattt cgcggttgat cttgcgcgcg
     4381 ccgtccttgc cgccctggct cttccacagc gaagcgcgat agttgaggtc ataggacacg
     4441 acggtgccgt atttatgcgc cgcctgcacc gcttcgatca ccgcttccga ggtgttgggg
     4501 gcgagcgcgg cgaaaatgcc gccggtatgg aaccagcgca cgccttcctc gccgaacagc
     4561 ttgtcccagt ccacttcgcc gggcttgatc tgcgacgcgg cggagttgcc gcggtcggag
     4621 cagcccagcg cggggcggat gccgtaaccc ttttcggtga agttgaggcc gacgctggtg
     4681 ttgcggccca gcccgtcgaa ttcgcgccag acgatgtgcg aagtatcgac gccgccttgg
     4741 cagatgaagt cttccaccag ccagcccaga tcgttgaccg gcagcgccgt caccacgctg
     4801 gagcgcttgc cccagcattt gcgcatggcg cgcgcgacgt tgtactcgcc gccgccttcc
     4861 cagacctgga actgccgcgc attgcgcacc cggccgaagc ccggatcgaa gcgcagcatc
     4921 acttcgccga aggagacgca atcccacttg cattcgccgg cggggcggat tttcaggatg
     4981 tcatcggcca ttgttatttc ccacggattt tcttgatgag cgcgatggtc tcggcgacct
     5041 tcgtctcgat gcccttgtag tccttggcct tgagcagctc ggaagtgatc agcttggagc
     5101 cgatgccggc cgcgacgatg ccggcgccga accatttctt cagcgattct tcggtgggat
     5161 cgacgccgcc ggtcggcatg atcttggtcc acggcatcgg gccgagcacc gccttgacga
     5221 attccggccc gcccacctgc gtacccggga atatcttcac gatctcgacg cccagttctt
     5281 cggcgtagga aatctcggag gccgaaccgc agccggggct gtagggaatc ttgcggcggt
     5341 tgcagacttt ggccacgtcg gcattcagca ccggaccgac gacgaaattc gcgccgttgg
     5401 cgatatagat gcccgccgtc ggcgcatcca gcaccgaacc gacgcccatg atcacgctct
     5461 tgtccgcctt ggcgaaatgg cgtgtcacct cgaggaagac atggggggcg aaatcgccgc
     5521 ggttggtgaa ctcgatgcac ttggccccgc cgtcggcgca agcctgaatc acgttggtgc
     5581 agacctcgac gtcgggatga tagaaaacgg ggataacccc ttgctccatc atcgccgcca
     5641 agacggtcat gcggtcctta gccattccct ggcaatcaag ttgaagcttt ggaagatgaa
     5701 accgatttcc t
//
LOCUS       DASWPU010000014         1579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_30719,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000014 DASWPU010000000
VERSION     DASWPU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30719"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..800)
                     /locus_tag="VGC27_00270"
     CDS             complement(18..800)
                     /locus_tag="VGC27_00270"
                     /inference="COORDINATES: protein motif:HMM:NF005095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0281045.1"
                     /translation="MSFSFELTGRRALVTGGTKGVGAAIVTVLRDEGVKVITTARSVP
                     ETSPEGVRFVAADLTTANGCAVVADAVVEQLGGIDIIVDVLGGSSAPAGGFAALDDEE
                     WSKEINQNLMAAVRIDRMLLPSMIEQGSGVIIHVTSIQHELPLPESTTAYAAAKAALA
                     TYSKSMSKEVTPKGVRVVRVSPGWVETEAAVALAERLADQAGTDYEGGKQIIMKSLGG
                     IPIGRPSTPHEVASLVGFLASPRAATITGTEYVIDGGTVPTA"
     gene            complement(797..1123)
                     /locus_tag="VGC27_00275"
     CDS             complement(797..1123)
                     /locus_tag="VGC27_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0281046.1"
                     /translation="MSFDLPASVAAYFAADKGNVETFAKCFTDDAVVKDEGHTYIGIN
                     EIRRWKAETSSKYTYTSEPFASEERGEKIVVTSRLTGNFPGSPVNLHFFFELEHDKIA
                     SLEIVS"
     gene            1265..>1579
                     /locus_tag="VGC27_00280"
     CDS             1265..>1579
                     /locus_tag="VGC27_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0281047.1"
                     /translation="MTPTSAADGVEQALKILEGRWKLVILFHLFGGRVLRFSDLERAI
                     PAISQKMLIQQLRQMEKDGVVRRIVHHQVPPKVEYSLTDWGQALCPALDALLTWAAQR
                     EAM"
BASE COUNT          322 a          458 c          473 g          326 t
ORIGIN      
        1 gtttcgcgga cgaaaagtca ggctgtagga acggtaccgc cgtcgatgac gtattcggtt
       61 ccggtgatgg ttgccgcgcg cggcgaggcc agaaatccaa cgaggctcgc gacctcatgt
      121 ggcgttgagg gtcggccgat cggaatacca cccaacgatt tcatgatgat ctgcttacca
      181 ccctcatagt ccgtgccggc ctgatccgcg aggcgctcgg cgagcgcaac cgccgcttca
      241 gtctccaccc agcccggcga gacgcgcacc acgcggacgc ccttcggcgt gacctctttc
      301 gacatgctct tactatacgt cgcgagcgcc gccttcgcgg ccgcataggc cgtcgtcgat
      361 tcagggagcg gaagctcgtg ctgaatcgaa gtgacatgaa tgatgacgcc cgagccctgc
      421 tcgatcatcg acggcaaaag cattcggtcg attcgaacag ccgccatcag gttctggttt
      481 atctccttcg accattcctc gtcgtcgaga gccgcgaagc cgcccgccgg cgcgctcgat
      541 ccgcccagca catcgacgat aatgtcgatc ccgccaagct gctcgacaac ggcgtcggca
      601 acaacggcgc agccgttcgc cgtcgtaagg tctgccgcga cgaagcgcac gccttcaggt
      661 gatgtctctg ggaccgaacg ggcggtcgta atgaccttga cgccttcatc gcggagcacc
      721 gttacgatcg ccgcgccgac gcccttcgtg ccgcccgtaa cgagtgcgcg gcgacccgtg
      781 agttcgaagc tgaagctcac gaaacgatct ccagcgacgc aattttgtcg tgttcaagtt
      841 cgaagaagaa gtggagattg acagggctgc ccggaaaatt tccggtaagc cggcttgtga
      901 cgacgatctt ttcgcctctt tcttcggatg cgaagggctc gctcgtatag gtatattttg
      961 atgaagtttc ggccttccac cgtctgattt cattgatgcc gatatatgtg tgaccttcgt
     1021 ccttcacgac ggcgtcgtcg gtgaagcact tcgcaaaggt ctcgacgttg cccttgtctg
     1081 ccgcgaagta tgcggcgacg gatgctggca gatcaaaaga catgagtttc tccttggcgt
     1141 cacgcgacga ccggatcgtc ttcgtgatgc tttgattgct tgcacaagag gatggagata
     1201 ggctcctaaa atggcaagaa cgcacgaaaa agtaaggaac acaccaaaag gtaaggatca
     1261 cgagatgacg ccgacgtcgg cggcggatgg cgttgaacag gcgctgaaga ttctcgaagg
     1321 ccggtggaaa ctggtaatcc tgttccacct gtttggcgga agggtactgc gcttttccga
     1381 tcttgagcgg gcgatccccg ccatctcgca gaagatgctg atccagcagc ttcgccagat
     1441 ggagaaggat ggggtcgttc ggcggatcgt ccatcatcag gtgccgccga aggtcgaata
     1501 tagcctgacg gattggggac aggcgctttg cccggcgctc gatgcgttac tgacatgggc
     1561 cgctcaacgc gaggcgatg
//
LOCUS       DASWPU010000015         3984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_104086,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000015 DASWPU010000000
VERSION     DASWPU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104086"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VGC27_00285"
     CDS             <1..777
                     /locus_tag="VGC27_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0281048.1"
                     /translation="GRATHDILIEAVGIVGYIGHVSLEWLEAWYAPRGNLRLTALVRQ
                     IEEVGINALPIIGLLMFLMGIVLAYQGADQLKRFDLEILTINALGIGVLREIGVLFTA
                     IIVAGRSGSAFTAQIGTMKVNEEIDAMQTIGLNVIDVLILPRVIALVIALPLLTFFAD
                     IMAMIGGAVMCYFQLGITVPAFMRQLHDAVTINTLLVGLIKAPVFAYVIAMVGCYEGM
                     NVERNAASVGKLTTRSVVEAVFLVIVLDAGFSVLFSMLGI"
     gene            778..1548
                     /locus_tag="VGC27_00290"
     CDS             778..1548
                     /locus_tag="VGC27_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0281049.1"
                     /translation="MSGAGKETVIRLRGVVSRIGGNLIHDNLDLDVYRGEVLGIVGGS
                     GSGKSVLLRSIVGLRRPQAGSIEVFGHDVLNAGESALRALQIRWGVLFQEGALFSSQT
                     VAQNIQVPLNEYTAMSQQLKNEVAAMKLSMVGLPGDTAQKRPSELSGGMKKRAGLARA
                     LALDPELVFLDEPTSGLDPISANMFDELVRGLQKSLNLTVFMVTHDLDTLKSIADRVA
                     VLVNGKLTVGTIDSLRNEKDPWIREYFSGARGRAALSS"
     gene            1558..2406
                     /locus_tag="VGC27_00295"
     CDS             1558..2406
                     /locus_tag="VGC27_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY0281050.1"
                     /translation="METKANYVAVGAFVLTCMLGLVIALLWLSGLQYSQEFSYYQTYF
                     TGPVTGLGKGTAVRYNGINVGRVNDVQFDPDDPQKVIVTLQVQPKLGIRVDSVISIES
                     EGLTGGSYVQIIGGKKDAPLLTPAEGQRYPVIKSAPSALQKLAKGVPQLLEKLNTAAD
                     RINAILNDDNRKAFARILTNLDQTTGALARRSDDIDSALADFSKASKALPGAVASIDE
                     SAKKFGQLSEDANEFVKGEGLTQFAELLADTRRLVVSLTKLSDELNRQPTKILFGDRR
                     KGYTPK"
     gene            2403..3035
                     /locus_tag="VGC27_00300"
     CDS             2403..3035
                     /locus_tag="VGC27_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEY0281051.1"
                     /translation="MTTTPSLLISRRLLLAGVSGLALAGCGRLVGPGEPPRLYLLKPA
                     LPQAAPGAKVDWALAIAVPDASAGLDSQRIAILRPPATLDYYAGAAWSDRLPPLVQNA
                     LLQAFEGCGRIDKVAPDSDGTHADYLLATDLRDFEARYDQPDGVPMAVVRIGVRLVRS
                     VKRNIAGRFEAAEEVPAAQNSIDAAVAALDEALARALAKIVAWTLEAPKP"
     gene            complement(3119..3208)
                     /locus_tag="VGC27_00305"
     tRNA            complement(3119..3208)
                     /locus_tag="VGC27_00305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3172..3174),aa:Ser,seq:cga)
     gene            complement(3288..>3984)
                     /gene="rlmJ"
                     /locus_tag="VGC27_00310"
     CDS             complement(3288..>3984)
                     /gene="rlmJ"
                     /locus_tag="VGC27_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEY0281052.1"
                     /translation="DSHAGRGVYDLRGKEAERTGEAASGIGRLASLSGGPPALAAYLE
                     LARTEGTYPGSPLIAAKLLRPQDRLVAIEKHPEDAAALAVALGPFRKARAENADGYAR
                     LLALLPPPERRGAVLIDPPYEAPGEFAEAARAVANALRRFATGIYLVWFPVKSRAEAD
                     GFCGEVLAAGARKALRLDVLLGERREDRLTAAGLAVVNPPFGFEDEMRAALALALPPM
                     GATARFHWLARGE"
BASE COUNT          728 a         1297 c         1268 g          691 t
ORIGIN      
        1 gggcgggcca cgcacgatat cctgatcgaa gcggtgggca tcgtcggcta tatcggccat
       61 gtcagcctgg aatggctgga ggcgtggtac gccccgcgcg gcaacctgcg gctgaccgcg
      121 ctcgtccgcc agatcgaaga agtcggcatc aacgcgctgc ccatcatcgg cctgctcatg
      181 ttcctcatgg gcatcgtgct cgcctatcag ggcgcggacc agttgaagcg tttcgatctc
      241 gaaatcctca ccatcaacgc gctcggcatc ggcgttctgc gcgagatcgg cgtgctgttc
      301 accgcgatca tcgtggccgg tcggtcgggc tccgccttca ccgcccagat cggcacgatg
      361 aaggtgaacg aagagatcga cgccatgcag acgatcggcc tcaacgtcat cgacgtgttg
      421 atcctgccgc gcgtcatcgc gctggtcatc gccttgccgc ttctcacctt cttcgccgac
      481 atcatggcca tgatcggcgg cgcggtgatg tgctacttcc agctcggcat caccgtcccg
      541 gcgttcatgc gccagttgca cgacgcggtg acgatcaaca ctttgctggt ggggctgatc
      601 aaggcgccgg tgttcgctta cgtcatcgcc atggtcggct gctacgaagg gatgaacgtg
      661 gagcgcaacg ccgccagcgt cggcaagctg acgacgcgct cggtggtcga agcggtgttc
      721 ctggtgatcg ttctcgatgc gggattttcc gtgctgttct cgatgctggg gatctgaatg
      781 agcggcgcgg gcaaggagac cgtcattcgc ctgcgcggcg tggtgagccg catcggcggc
      841 aatctgattc acgacaacct cgatctcgac gtctatcgcg gcgaggtgct gggcatcgtc
      901 ggcggctcgg gttcggggaa atccgtgctc ttgcgctcca tcgtcggcct caggcggccg
      961 caggcgggaa gcatcgaggt gttcggccac gacgtgctca atgcgggaga atccgccctg
     1021 cgcgcgctgc agatacgctg gggcgtcctt ttccaagagg gggcgctgtt cagctcgcag
     1081 accgtggcgc agaacatcca agtccccttg aacgaataca ccgccatgtc gcagcaattg
     1141 aagaacgaag tcgccgcgat gaagctctcg atggtgggcc tgcccgggga cacggcgcag
     1201 aagcgcccgt ccgaactttc cggcggcatg aagaagcgcg ccggcctggc gcgcgcgctg
     1261 gcgctcgatc ccgaactcgt gtttctcgac gagccgacgt ccggcctcga tccgatctcg
     1321 gcgaacatgt tcgacgaact ggtgcgcggg ttgcagaaaa gcctgaacct caccgtgttc
     1381 atggtcacgc acgatctcga cacgctgaaa tcgatcgccg accgcgttgc ggtgctggtg
     1441 aacggcaagc tcacggtggg aacgatcgac agcctgcgca acgaaaagga tccctggatc
     1501 cgggaatatt tctccggcgc gcgcggacgc gcggcactct cgtcatgagg tgacggcatg
     1561 gaaacgaaag caaattatgt cgccgtaggc gccttcgtgc tgacctgcat gctggggctg
     1621 gtcatcgccc tgctttggct gtccggcctg caatacagcc aggaattctc ctattatcag
     1681 acctatttca ccggtccggt caccgggctc gggaaaggca cggccgtccg ctacaacggc
     1741 atcaatgtgg gccgcgtcaa cgacgtccag ttcgaccccg acgatccgca aaaggtcatc
     1801 gtcacgctcc aggtccagcc caagctcgga attcgcgtcg actcggtcat ctcgatcgaa
     1861 agcgagggcc tgacgggcgg ttcctacgta caaatcatcg gcggcaagaa agacgcgccg
     1921 ctgctgacgc cggcggaagg ccagcgctac ccggtgatca aatcggcgcc ctccgcgctg
     1981 caaaagctgg cgaagggcgt gccgcagctg ctcgagaaac tcaacaccgc cgccgacagg
     2041 atcaacgcga tcctgaacga cgacaaccgg aaagccttcg cccgcattct gaccaacctc
     2101 gaccaaacga cgggagcctt ggcgcggcgc tcggacgaca tcgattccgc ccttgccgac
     2161 ttctccaaag catcgaaggc cctgcccggc gcggtcgcca gcatcgacga gagcgcaaag
     2221 aaattcggtc agctttcgga ggacgccaac gaattcgtca aaggcgaagg gctgacccag
     2281 tttgccgaac tgctcgccga caccagaagg ctcgtggtga gcctcaccaa actttcggac
     2341 gaactcaacc ggcagccgac gaagattttg ttcggcgacc ggcgcaaggg atacacgcca
     2401 aaatgacaac gactccgtct ttgctgataa gccgccgcct gctgctggcg ggcgtttccg
     2461 gcctggcgct tgccggctgc ggccgcctgg tcggccccgg agagccgccc cggctttacc
     2521 tcctcaaacc cgcgctgccg caagcggcgc cgggagccaa agtggattgg gcgcttgcga
     2581 tcgccgtgcc cgatgcctct gccggcttgg actcgcagcg tatcgcgatt ttgcgtccgc
     2641 cggcgacact ggattattac gccggcgcgg cttggtcgga ccgactgccc cctctggtgc
     2701 agaacgccct gctgcaggcc ttcgagggct gcggacgtat cgataaggtg gcgcccgaca
     2761 gcgacggcac ccatgccgac taccttctgg cgaccgatct gcgcgacttc gaagcgcgct
     2821 acgatcagcc ggacggcgtg ccgatggcgg ttgtacgcat cggcgtcagg ctggtccgca
     2881 gcgtgaaacg caacatcgcc ggccggttcg aagcggccga ggaagttccg gcggctcaga
     2941 attcaatcga cgcggcggtt gcggcgctcg acgaagcgct ggcccgcgcc ttggcgaaaa
     3001 tcgtggcctg gacgctggag gcgccaaagc cttaaagcgg atcgctgggc acggcaggag
     3061 ggaccgccgc tctccgcttg gcatgagtta agctattaat atcgaacatg aaaatcattg
     3121 gcggacaggg ggggattcga acccccggta cggttacccg tacgccgcat ttcgagtgcg
     3181 gtgctttcaa ccgctcagcc acctgtccgt cgagccggat caaatactta agccggaggg
     3241 ggatggcaag ccgggcggaa ttggcgctgc gcgaggcgtg ccggcgtcta ttctccgcgc
     3301 gccagccaat ggaaccgggc cgttgcaccc atcggaggaa gcgcgagcgc cagcgccgcg
     3361 cgcatttcgt cttcaaagcc gaacggcgga ttgacgactg ccagacccgc cgccgtcaga
     3421 cggtcttcgc gccgttcgcc caacaggaca tccaggcgca atgccttgcg ggcacccgcc
     3481 gccagcactt cgccgcaaaa gccgtccgct tcggcccgcg atttgaccgg aaaccagacg
     3541 aggtaaattc ccgtcgcgaa acggcgcaaa gcgttcgcca ccgcgcgtgc ggcctcggcg
     3601 aattcgccgg gggcttcgta aggcggatcg atcaggacgg cgccgcgccg ttcgggcggc
     3661 ggcagcaggg caagaagacg cgcataacca tcggcgttct ccgctcgcgc tttgcggaac
     3721 ggccccagcg cgacagccag cgccgctgcg tcttcgggat gtttctctat ggcgaccagc
     3781 ctgtcctgcg gcctcagcag cttggcggcg atcagcggcg aacccggata agtcccctcc
     3841 gtgcgcgcca gttcgagata ggccgcaagc gccggcggcc cgccgctcag agaagcgagc
     3901 cggccgatgc cgcttgccgc ttcgccggtg cgctccgctt ctttgccgcg aagatcataa
     3961 acgccgcgac cggcatggct gtcg
//
LOCUS       DASWPU010000016         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_97960,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000016 DASWPU010000000
VERSION     DASWPU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97960"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..643
                     /locus_tag="VGC27_00315"
     CDS             347..643
                     /locus_tag="VGC27_00315"
                     /inference="COORDINATES: protein motif:HMM:NF017717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HEY0281053.1"
                     /translation="MTIESVVKGVRAAQAAALRNIRLPEVRHKTGLSRPTLYRLIRQG
                     RFPAPGKAGCSSVWLESAVDEWVAACHAARVQAPVDTVAARTARKTKTAQTAEA"
     gene            643..1239
                     /locus_tag="VGC27_00320"
     CDS             643..1239
                     /locus_tag="VGC27_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281054.1"
                     /translation="MDDSNEKGRRTAHAMALESSNPIRVPNFTSTGVVKEGEVVSFVG
                     DACKDIVDFFNFCREHGLPEGCSLELDESEDDDDTELTLTIGRVRMQLSLGGYRPVAC
                     IDAHSWRSRARDIFEHGTETIDSVADELATRRQARTLLTARDLPEDARTMKRESDAVA
                     ETMCDLMDVLDDPRNWRDAIPSDVLILGRARLVKEVSA"
     gene            1236..>1707
                     /locus_tag="VGC27_00325"
     CDS             1236..>1707
                     /locus_tag="VGC27_00325"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEY0281055.1"
                     /translation="MNAAADHEAVALAAATPRTKLDHALALAARGFHIFPVRAKGDVY
                     KSKSGKTKLSDGKVPAVDDWPNKSTRYEAQIRRWWKSHPNRNIGIDTGKPLPDGRFPF
                     AVDVDVKGEKQGEASLRKLIAEVGAGPMSTWEQATPSGGMHLLFSTERPMKQGVD"
BASE COUNT          353 a          510 c          559 g          285 t
ORIGIN      
        1 gatctggaaa tggacgaaga cctgtgaggc ggtacgttac gattaccgta acataaatta
       61 aatcaacaac ttaggcttgc ttgagcttga aagagacggt atagtctcgg acatcgctga
      121 accaaagtaa tttggcggtg gtagttgcta cagggggccg cagagccggc cggcggtgga
      181 aggttcagag agttcaccgc ataccgccag cgcgagctgg aatagcccaa gggcttaccc
      241 gacacacaac acctccgcag atcccccgtc agggccggga tctgcggagg tgtttgcatt
      301 ttgcgcgcct cgccgtggcc ggaaccaacg caaaggtctc cgaaccatga ctatcgaatc
      361 tgttgtcaaa ggtgtgcgtg ctgcgcaagc cgcggccctt cggaatatcc ggctgccaga
      421 ggtgcggcac aaaaccgggc tctcgcgccc aaccttgtat cgactaatcc gacaggggcg
      481 ctttcccgcg cccggcaagg ctggctgttc gtcggtgtgg ctcgagagcg ctgtcgatga
      541 gtgggtggcc gcctgccacg ccgcccgcgt ccaagcaccg gtggacaccg ttgcggcgag
      601 gacggcgcgg aagaccaaga ctgctcagac cgcggaggcg taatggatga ctccaacgaa
      661 aagggccgcc gcactgccca tgcgatggcc ctcgagagct caaacccaat ccgcgttccg
      721 aactttacca gcaccggcgt cgtgaaggaa ggcgaggtgg tgagcttcgt cggcgacgcg
      781 tgcaaggaca tcgttgactt cttcaacttc tgccgcgagc acggcctgcc cgaaggttgt
      841 tcgctcgagc tggacgaaag cgaggacgac gacgacaccg agctgacgct tacgatcggc
      901 cgcgtgcgga tgcaactgtc gctcgggggt tatcgccccg tggcgtgcat cgacgctcac
      961 tcctggcgta gcagagcacg cgatatcttt gaacacggca cggagacgat cgactctgta
     1021 gccgacgaac tcgccacgcg gcgccaggcc cgcacgctgc tcacggcgcg ggatctgccg
     1081 gaagacgcgc ggaccatgaa gcgcgaatcg gatgccgtcg ccgagacgat gtgcgatttg
     1141 atggacgtgc tcgacgaccc gcgcaactgg cgcgacgcca tccctagcga cgtgctcatc
     1201 ctgggccgcg cgcgcctggt caaagaggtg tcagcgtgaa cgccgctgcc gaccatgagg
     1261 ctgtcgcgct cgcagcggcg acgccgcgga ccaagctcga ccacgcgctg gcgctggccg
     1321 cgcgaggctt tcatatcttt ccggtgcggg ccaagggaga tgtctacaag agcaagagcg
     1381 gcaagaccaa gctgagcgac ggaaaggttc cggctgtcga cgactggccc aacaagtcca
     1441 cgcgctacga ggcgcagatt cgtcgctggt ggaaatcgca cccgaaccgg aacatcggca
     1501 tcgacactgg caagccgctg cctgacggcc ggtttccatt cgctgtagat gtggacgtga
     1561 agggtgagaa gcagggcgaa gccagcttga ggaagctgat tgctgaggtt ggcgccggcc
     1621 cgatgtcgac gtgggagcag gccacgccat caggtggaat gcacctgttg ttctcgaccg
     1681 agcgaccgat gaagcagggt gtcgatg
//
LOCUS       DASWPU010000017         8032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_12408,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000017 DASWPU010000000
VERSION     DASWPU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12408"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VGC27_00330"
     CDS             complement(<1..549)
                     /locus_tag="VGC27_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281056.1"
                     /translation="MGTSTVIGLLVLSFPLGMLHALDPDHVLAVATLTGREKTPSRAL
                     AYAVHWAVGHGGLLLLITAAAVIFHWVLPQEVPYWAERCVGVILILTGTSVLLNIRKE
                     RLHQHRHAGGFLRHSHEPAHEMEPGGDKEPSRTRSPLRAPLLIGIVHGTAGSAPFLAL
                     IPAAFMSPAKGILYVAIFSIGVL"
     gene            complement(671..1687)
                     /locus_tag="VGC27_00335"
     CDS             complement(671..1687)
                     /locus_tag="VGC27_00335"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEY0281057.1"
                     /translation="MKKQISRRGFLEKSAVVAGTVAASEFQGLGSAFAAPANTSPVYF
                     TKDITVAGLLKAYAKVNQGMTGKIAVKLHTGEPHGPNLLPIELIKGLQAHVTGSTIVE
                     CNVLYPSPRQVTETHRETLKTNGFDFCPVDIMDAEGDASLPVPGMQALLDKYADPAIT
                     KRPFTPGQHLTEVAVGKHLLNYDSLLVYTHFKGHTMAGFGGSLKNVAIGCASGKVGKL
                     QIHGEGFRRGDLFLERMVEAGKAICTHFGPHITYINVLKNLSVDCDCDAHGAKPTAKD
                     IGILASTDVLAIDKASLDMMYALPAGERHDIIERIESRDGPHQIEYMKTLGMGNERYE
                     LIAI"
     gene            complement(2780..3058)
                     /locus_tag="VGC27_00340"
     CDS             complement(2780..3058)
                     /locus_tag="VGC27_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281058.1"
                     /translation="MLLLARRQEILVPASAVEIPAAVKRPLGLDAERSWIVVAEGNEF
                     LWPGYDLRKLPQGDRYEYGFLPPHVFNQVRDAFVAYYRTGPHHIVPRN"
     gene            3376..3786
                     /locus_tag="VGC27_00345"
     CDS             3376..3786
                     /locus_tag="VGC27_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281059.1"
                     /translation="MKYEEFAANFANPEPVLREAATSDKSATAGAVIAGTVVSAHKDL
                     LHLDINGVQYELDPAEVIDVQLLAKPTAETCDKDRQEDAKGAAEASPATPSNIVLINA
                     HCGAVLYRRVAVPAAQIAALGTWVHVVPAASEAA"
     gene            3874..4110
                     /locus_tag="VGC27_00350"
     CDS             3874..4110
                     /locus_tag="VGC27_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0281060.1"
                     /translation="MRDIIVRYVEAISNEKGFDPATNLFEAGVLTSLDVLSLVAFLEE
                     TFDLSISGDDIDIDHFGTIDGLVALVSAKQSQKT"
     gene            4119..5633
                     /locus_tag="VGC27_00355"
     CDS             4119..5633
                     /locus_tag="VGC27_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0281061.1"
                     /translation="MLAVHDMLDRAARDDPTKIAIISRTASTSYAELDEVSTRIAGAL
                     RRRGVEKGMRVAIYASKSIQEVAVIFAVAKAGGVLVHINPAFRDEQLQHVLQETEPAA
                     LFFHPSKAAAIARAIDGGGRPPCLIALSGPAGTRLLPPCETLEALIATAGPVANGAAS
                     ENDLAAIIYTSGTTSRAKGIKVTHGILSQSTTVSADVLQNGPGDRLISLTPFSFDGAL
                     SQLFTATLVNATLFLQDSQFPKDVIHTLLTQKITGFHAVPSFWLMTLERYPAFAEYHF
                     PHLRYLSLIGEVFPEDALFRLKDILAQTGFYMMYGTTEAFRSTCLLPEDFTRKKGSAG
                     KPLPGVKIAIVGADGTPLKPGQTGEIVHSGAFVSPGYWHRDGDATFREDGVHTGDLGR
                     LDEEGYLYFAGRKDTMIKRLGYQVYPEEIEACLTKLDGVALAAVACAPGCANPLIRAF
                     ITRQPGSALTADDVTEHCRRHLPHYMRPDEVVFRVEMPTTGPNKIDRVQLSRSP"
     gene            5630..6655
                     /locus_tag="VGC27_00360"
     CDS             5630..6655
                     /locus_tag="VGC27_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281062.1"
                     /translation="MRRCTRCVLPDSFPGISFNNDGVCSYCTTFDAQASPRAAPGPLH
                     DTVLLLMEASKGQAKRYDAVVAFSGGKDSTFLLQVLKARFGLRLLAVTFDNGFLSKAC
                     TSNMRNVLTALDIDHLIIAYRRDHMNAAFTASALSRVYPDHLAMFGSGVCISCIRMVL
                     TAVLRTAIEKRIPMVMLGNSPGQILRSASEILYRDNTIPFAVRKALFAKLAERTGDWV
                     YHYVMLDADEYKTTPFPYIVNPLPVLGYDEAEIYRTIAGLGWSKPADVDPNSTNCRLN
                     AFGTIQHKNIYGFHPYDYEMSQLVRLGSLSGEAALERLTDPADTAIAIAADVEREVVC
                     HGCKGGR"
     gene            6660..>8032
                     /gene="asnB"
                     /locus_tag="VGC27_00365"
     CDS             6660..>8032
                     /gene="asnB"
                     /locus_tag="VGC27_00365"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY0281063.1"
                     /translation="MCGLCGILNMQKDSIVSTAVLSAMTVRLAHRGPDDQGIFTDRFV
                     GFGHCRLSILDVANGKQPLTNEDGTVWVTYNGEIYNYRELRRGLIAKGHHFVTDCDTE
                     VIVHAYEEYGERCLSQFNGMFAFALWDSRKQLLFLARDRQGIKPLHYAIFGSHFVFAS
                     EIKAILEYPEAKAEVDLNGIAEYLLCTTLLESRTMFKNIHSVPPGTAIIVEDGLIRRL
                     RYWAIERDSIRHTDSYEDCCERVRHVLHSAVSMEMISDVPLGTLLSGGLDSSLVSAIA
                     AKHACGCLKTFSMDYERNEHVRREPTDHHYAGLVSAAYDTDHTELVFRADDYFDALEK
                     TTWHVEKPVDLTAPSLYLLYRGVKPKVTVVMSGEGADELFAGYFFFLDYRLNGHPVEF
                     PWAPYLDAVSLILNPDIDRQCGYRDRIRATLAAETDWFETDDVVNTQLLLFLKYYLVD
                     MLERLDKT"
BASE COUNT         1646 a         2471 c         2320 g         1595 t
ORIGIN      
        1 cagaacgccg atcgaaaaaa tggcgacgta gagaatcccc ttcgccgggc tcatgaaggc
       61 ggcggggatc agcgccagga acggcgcact gccggcggtg ccgtggacga taccgatcag
      121 taacggggcg cgcagcggcg aacgcgtgcg agagggctcc ttgtcgccgc ccggctccat
      181 ctcgtgcgcc ggttcgtggc tatgccgcaa aaaaccgccg gcgtgcctat gctgatggag
      241 acgttctttc cggatattga ggagcacgga ggtgccggtg aggatcagga tgaccccgac
      301 gcaacgttcc gcccagtacg gcacttcctg cggcagcacc cagtgaaaga tgaccgccgc
      361 ggcggtgatc aacaacagca atccgccgtg gccgaccgcc caatgcacgg cgtaggcgag
      421 cgcgcgcgac ggcgtcttct cgcggccggt gagcgtcgcc accgccagca cgtgatccgg
      481 atccagggcg tgcagcattc cgaggggaaa gctgagcaca agaagaccga tgacggtaga
      541 tgttcccatg ttcgtcctca acccggccgt gcgggcaatc tgcggccact tccgtttcgt
      601 acgttgaccg gcggaagata gcaacgaccg gcgatgcgaa cgccgtcaat atcgccggcc
      661 gcagggcagg tcagatcgcg atcaactcgt accgctcatt gcccatgccg agcgtcttca
      721 tgtattcgat ctgatgcggg ccgtcgcggc tctcgatgcg ttcgatgatg tcgtgccgct
      781 cgcccgcagg cagcgcgtac atcatgtcga gcgaagcctt gtcgatggcc aggacgtcgg
      841 tcgaggcgag aatgccgatg tccttcgccg tcggcttggc gccgtgggca tcgcaatcgc
      901 agtccaccga gaggttcttg aggacgttga tataggtgat gtgcggccca aaatgggtac
      961 agatcgcctt gccggcctcg accatgcgct cgaggaaaag atcgccccgc ctgaagcctt
     1021 cgccgtggat ctgcagcttg cctaccttgc ccgacgcaca gccgatggcg acattcttga
     1081 gcgagccgcc gaacccggcc atcgtgtggc ctttgaaatg ggtgtagacc agcaatgaat
     1141 cgtagttgag cagatgcttg ccgaccgcga cttcggtcag atgctggccc ggggtaaaag
     1201 gacgcttggt gatcgcggga tcggcatact tgtccagcaa cgcctgcatc cctggaaccg
     1261 gtaatgaagc gtcgccctcc gcatccatga tatcgaccgg gcaaaaatcg aaaccgttgg
     1321 tcttcagggt ttcccgatgc gtctcggtta cctgccgcgg gctgggataa agaacattgc
     1381 attccacgat cgtgctgccg gtgacatgcg cctgcagccc cttgatcagc tcgatcggca
     1441 acaggttcgg accgtgcggc tcgccggtat gcagcttgac ggcgattttg ccggtcatgc
     1501 cctgattgac cttggcatag gccttcagca acccggcaac ggtgatgtcc ttggtgaaat
     1561 agaccggcga cgtattcgcc ggcgcggcaa aagcgctgcc caaaccctga aattcgctcg
     1621 cggcgacagt cccggcgaca accgcactct tctccagaaa gccgcgacgc gatatctgct
     1681 ttttcatggt gatccctcca atcgcaggac ggtttaggat accatttccg ctgtatttct
     1741 ttaattcaaa cgccgatcac tttatcgttc aagcggtctg gcaatccgcg tgaaatgtta
     1801 ccctgatacc aaatccgtgc tgccaagcgt gtagcagctt ccaatgacag cttaggcggc
     1861 cgaaacggct tcgaacgtgg gacaagggga cgcattgaat atgcctgtga gcgacacccg
     1921 tcccgaatgc gtgcctgccc aagcaagttc aatcgatgcg agccaacgtg catggccagg
     1981 ccaagagtga cggatgtagt cgctgtccgc tcttatggtg attgtcgggc acgccgtttt
     2041 tttgaatatc gccgtccctg atgccgcccc tcaaagatgt cgccctgacc agggcaccca
     2101 attttccagc aaatcagaat gagcgtccgg cctgcttacc ggcgcagaaa tttgttgcgg
     2161 catgaatagg aattaagtca tggtgccagg gggtggcaca ccctttcccg cctcggaatc
     2221 gaaccaccgc ccaggtgggg tatccggtag ctgacctttg tcgccacggg agagacttga
     2281 gtggttcata aaaacctgcc cgaaggcgac gattgtgaag acgataatcc cgttgggggt
     2341 ggcccatggt catgatcgaa cgtctcaaga aagatgagac gccgccatcc gggtcaatcc
     2401 gctcgggccg cgcagcagga ttttcggtat ggcctcacta attttcagcg cttcgaattc
     2461 gtccaagtgc gcgtaattcg gacaccccga tagaggaagc tactggatct ccagttactg
     2521 acagatttac cgcgtttacg gacagcaaca ttcattgccg cgcccataaa gttgaacctt
     2581 tttcagcgct ttctaaagga gagaaaggct tatcggaacg aaagtctagc cgctggccca
     2641 atgagtgtct atgagcccca ttgtgcaaca atatgcatta cagatgatct cgttggttgg
     2701 gtcagacaga gtgtttgcgc atacccccat ccgactgctg tcagcagcag ccgattatcg
     2761 cgaattacat tcacatttat tagttacgcg gtacgatatg atgcggaccg gtccgatagt
     2821 aagcgacgaa agcatcgcgg acctgattga agacgtgcgg cggcaggaag ccgtattcat
     2881 agcggtcgcc ttgcggcaat ttgcgcaaat cataacccgg ccagagaaat tcgttacctt
     2941 cagcgacaac gatccatgaa cgctccgcgt cgagtccgag aggccttttg actgccgctg
     3001 gtatctcgac ggccgatgcc ggcaccagaa tttcctgacg tcttgccaag agcagcatca
     3061 gatattgact aaaatcaatg ataggttgag tatatagaaa tatacatttt tttgttgaat
     3121 gccatggaag gcgttgtgtt tatggcgctt ttcagtcatg ggcggttggg gacgcaggca
     3181 gtattgggca aggatcgctt gccgcgcttt aactacgcac ccctctccca taggttgtgc
     3241 agtgtactca tgtccttgta gggcgcttgt tcggtcgcgc ccgcacatgc cgagccgctt
     3301 aatgaacagg cacggttcaa gcaatggaat tcccgcgagc cagatctcgc ggcaaccaac
     3361 caatggggga cagtcatgaa gtacgaagaa tttgccgcca actttgcgaa tccggaaccg
     3421 gtcttgcgcg aagccgcaac ctcggacaaa agcgcgacgg ccggagccgt cattgcgggc
     3481 accgtcgtga gcgcccacaa ggacttgctt cacttggata tcaacggtgt gcagtacgaa
     3541 ctcgatccgg ccgaggtgat cgacgttcag ctcctcgcca agccaacggc cgaaacgtgc
     3601 gacaaagaca ggcaggaaga tgcgaaaggc gcagcggaag cctcgccggc gacgccatcg
     3661 aacattgtcc tgatcaatgc gcattgcggc gccgtgttgt ataggcgcgt ggccgttccc
     3721 gccgctcaga tcgccgcctt gggcacctgg gtgcatgtgg tgcccgctgc gagcgaagcg
     3781 gcctagagag acatcgtcca actcctgaat gacaggaggc gagcggctcg ccagttcctt
     3841 gcgaaggcgc tcgtccgggg gcatcagatg aacgtgcgcg acatcatcgt gcggtatgtc
     3901 gaagcgattt cgaacgaaaa aggcttcgat cccgcaacca atctcttcga agccggcgtg
     3961 ttgacatcgc tagatgtcct ctcgcttgtc gccttcctcg aggagacgtt tgacctgagc
     4021 atttcgggtg atgatatcga catcgatcat ttcggcacca tcgacgggct ggtggcgctg
     4081 gtctcggcca agcagtcgca aaagacttag gtgtcccaat gttggctgtt cacgacatgt
     4141 tggacagggc ggcacgggac gacccaacca agatcgccat catctcgagg accgccagca
     4201 cttcctatgc ggagttggac gaagtctcga cgcggatagc cggtgctctg cgccgccgcg
     4261 gtgtggaaaa aggcatgcgc gtcgcgatct acgcgtcaaa atcgatccaa gaggtggcag
     4321 tcatcttcgc cgttgctaag gccggcggcg tgctcgtgca catcaatccg gcatttcgcg
     4381 acgaacaatt gcagcatgtt ctccaggaga ccgaaccggc ggcgctcttc ttccatccca
     4441 gcaaggccgc ggccatcgcg cgcgccatag acggcggcgg gcgccctccc tgcctgatcg
     4501 ccctgagcgg tccagcggga acacgcctgt tgccgccttg cgaaacactc gaggcgctga
     4561 tcgcaacggc cggcccagtt gccaatggcg ccgccagcga gaacgacctt gcggccatca
     4621 tctacacctc cggaacgaca tcccgcgcca aggggatcaa ggtcacgcac gggattttat
     4681 cacaatcgac gacggtctcc gccgacgtcc ttcagaacgg cccgggcgac cggctgatca
     4741 gtctcacgcc cttctccttc gacggcgcct tgagccagct tttcaccgcg acgctggtca
     4801 acgccacgct cttcctgcag gactcgcaat ttcccaagga cgtcatccac acgctgctga
     4861 cgcagaagat caccggcttc catgctgtgc catcgttctg gctgatgacc ctcgagcgct
     4921 atccggcctt cgcggagtac cattttccgc atctgcgtta tctctcgctg atcggcgaag
     4981 tgtttcccga agacgcgctc ttcaggctca aggacatctt ggcgcaaacc ggtttctaca
     5041 tgatgtacgg aacgaccgag gcgtttcgct ccacctgcct gctgccggag gatttcacac
     5101 ggaagaaagg ttccgccggg aagcccctgc ccggtgtgaa gatcgccatc gtcggtgctg
     5161 acggcacccc gctgaagccc ggacagaccg gggagatcgt gcatagcggg gcattcgtct
     5221 cgccgggtta ctggcaccgc gacggcgatg ccacattccg tgaggatggc gtccacaccg
     5281 gcgatctcgg acggctggac gaagagggat atctctattt cgccggccgc aaagacacca
     5341 tgatcaagcg cctcggctat caggtctatc ccgaggagat cgaggcgtgc ctcacgaagc
     5401 tcgacggtgt ggctctggcc gccgtcgcct gcgccccggg atgcgccaat ccgctgatcc
     5461 gcgccttcat cacgcggcag cccggctcgg cgctgacggc cgacgacgtg accgaacatt
     5521 gcagacgcca cctgccgcat tacatgcgcc ccgatgaagt cgtcttccgc gtcgagatgc
     5581 ccactaccgg tccaaacaag atcgaccgtg tccagctttc gaggtcgcca tgagacgctg
     5641 cacgcgctgt gtcctgccgg acagctttcc tggcatctcg ttcaacaacg acggtgtctg
     5701 ctcgtattgc acgacgttcg acgcgcaagc ctcaccgcgt gcggcgcccg gcccactgca
     5761 cgatacggtg ctcctgctga tggaagcctc gaagggacag gccaagcgct atgacgcggt
     5821 cgtggccttc agtggaggca aagacagcac cttcctgctg caggtgctga aggcgcgctt
     5881 cgggctccgg ctgctggcgg tcaccttcga caacggcttc ttgtcgaagg cgtgtaccag
     5941 caacatgcgc aatgtcctta ccgcgctcga catcgaccat ctgatcatcg cataccgccg
     6001 cgaccatatg aacgccgcct tcacggccag cgcgctgtcc cgcgtctatc ccgaccacct
     6061 tgcgatgttc ggcagcggcg tctgcatctc ctgcatccgg atggtgctga ccgcggtact
     6121 ccgcacggcc atcgagaagc ggattcccat ggtcatgctc ggcaacagtc cgggtcagat
     6181 tctgcgctcc gcgagcgaaa tcctctaccg ggacaacacc attccgtttg ccgtgcgcaa
     6241 ggcgctgttc gctaaactgg ccgagagaac cggggactgg gtctatcact atgtgatgct
     6301 cgacgccgac gagtacaaga ccacgccgtt cccctatatc gtcaatcccc tgcccgttct
     6361 cggctatgac gaagcagaaa tctaccgcac catcgccgga ctcggctgga gcaagcccgc
     6421 agacgtcgat cccaattcga caaactgccg tctcaacgcc ttcggcacca tccagcataa
     6481 gaacatctac ggctttcatc cctacgacta cgagatgagc cagctcgtcc gtctgggctc
     6541 gctcagcggg gaggcggcgc tggagcggct caccgatccg gccgacaccg ccatcgccat
     6601 cgcggccgat gtcgagcggg aagtcgtctg ccacggctgc aaagggggcc ggtaggacca
     6661 tgtgcggact ctgcggcatc ctgaacatgc agaaggacag catcgtctcg acggcggtgc
     6721 ttagcgcaat gacagtgcgt ctggcccatc gcggaccaga cgatcaaggc attttcaccg
     6781 accgcttcgt cggcttcggc cactgccgcc tttccatcct cgatgtcgcc aacggcaagc
     6841 agcctctcac taacgaagac ggcacggtgt gggtcacgta taacggagaa atctacaact
     6901 accgggaatt gagacggggc cttattgcca aaggccatca cttcgtcacc gattgcgaca
     6961 ccgaggtcat tgtccacgcc tatgaggaat acggcgaacg ttgcctgtcg cagttcaatg
     7021 gcatgttcgc cttcgcgctg tgggacagcc gtaagcaatt gctctttctg gcgcgggacc
     7081 ggcaaggcat caagccactg cactatgcca tcttcggttc ccacttcgtc ttcgcgtccg
     7141 agatcaaggc gatattggaa tatcccgagg ccaaggcgga ggtcgatctc aacggcatag
     7201 cggaatacct gctgtgcacg acgcttctcg aatctcggac gatgttcaag aacatccatt
     7261 ccgtcccgcc gggcacggcg attatcgtcg aggatggcct cattcgcaga ctgcgctact
     7321 gggccatcga gcgggattcg atacggcata ccgattccta tgaagactgc tgcgagcggg
     7381 tccgtcatgt tctgcattcg gccgtaagca tggaaatgat cagcgacgtg ccgctcggca
     7441 cgctcctcag cggcggcctc gattccagcc tcgtctccgc catcgccgcg aaacacgcct
     7501 gcggctgcct gaagacgttc tcgatggact acgaacggaa cgaacatgtc aggcgcgagc
     7561 ccactgacca ccactacgcc ggtctggtgt cggctgctta tgacacggac catacggagc
     7621 ttgtcttccg ggccgacgac tatttcgacg cgctggagaa gacgacgtgg cacgtcgaga
     7681 agccggtcga cctgaccgcg ccttctttgt acctcctcta ccgcggggtg aagccgaagg
     7741 tcaccgtcgt gatgtcggga gaaggcgccg acgaactctt tgccggctac ttcttcttcc
     7801 tcgattatcg cctgaacggc caccccgtcg agttcccctg ggccccctat ctcgacgcgg
     7861 tatcgctcat cctgaatccc gacattgaca gacagtgcgg ctatcgcgac agaattcgcg
     7921 ccacgctcgc ggccgagacg gactggttcg agacggacga cgtcgtcaac acgcagctgc
     7981 tgctgttcct gaaatactat ctggtcgaca tgctcgagag gctcgacaag ac
//
LOCUS       DASWPU010000018         4444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_61347,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000018 DASWPU010000000
VERSION     DASWPU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61347"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..477)
                     /locus_tag="VGC27_00370"
     CDS             complement(109..477)
                     /locus_tag="VGC27_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281064.1"
                     /translation="MNELLTRRNILIGAGGAVALSAVALEGRRLLRKRYAPSPYDDLL
                     TLLDDRDADAQIGEAVLADLDDFDARIVADDLRERIGDRPLAQVMAEDVAEGRTLEAN
                     GWVIPETLALLCALAAKAAA"
     gene            complement(474..1967)
                     /locus_tag="VGC27_00375"
     CDS             complement(474..1967)
                     /locus_tag="VGC27_00375"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY0281065.1"
                     /translation="MAAFIDARTVPEGTVVETDLAIVGGGPVGISLALTLAATPLRMV
                     LLESGGMTFDAKTQALYEGSKVGAPYLTLDATRLRYLGGSTNHWGGWCRPMDKIDFEE
                     RTWMPYSGWPFGREALDPYYARAQSLCEAGPFLYDRIKEWAPAPLLALGEGGLTTRWF
                     QFSKMRGSVLPTHFGDRYAEDLKRIARLSTYLHANVTRLGLDGSAARIGELDVATLTG
                     RKFKVKPKITVLAMGAIEIARLMLASNDVMPAGVGNAHDLVGRFFADHPIPRDAVTLV
                     LFDGKLAPFYQSNQTIHGAIARAGLFPSEEFRRRHAVMDSSTTIENKTELDELGKAAV
                     AATAKALGVNAGSATAFSLGCGMEVTPDPNRRLILDSERDALGMPKLKLYMRIADSDF
                     ARFRLTLKEMGRQLLASRTGMLRLNQKERSQWLDGLDWASHHMGTTRMDADPHKGVVD
                     ANLQVHGVANLFVAGSSVYPTYSAVNPTLNLLALALRLADHLKGLRR"
     gene            2519..4270
                     /locus_tag="VGC27_00380"
     CDS             2519..4270
                     /locus_tag="VGC27_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY0281066.1"
                     /translation="MSDFSTEAAKDGLKLKLWRGERMCLVGMNVEAPEPDFVGFAIEV
                     KRPGAGGFMPLRNRLAFSYPQTPDASVTGARQFSSLEAPFQKFRWIHFPQEPQLGSYT
                     YRVTKLHMPGEGRLVAGTRLAAGISLGPVTYDGYLDIGFTRNFASSQIYAERYKNNPN
                     IIPTKADGGLEFKKVAGDVYRWLGFEAYQMIFDFLDEAVKDSSVSLDVLAYDLNEPDM
                     VAALEKLGRRLRIVIDDSKTHSAKTTAESKAAARLVKSAGAQNVRRTHFKSLQHHKVL
                     IAKRGGKPFKALFGSTNFSFRGLYIQANNALRFDAPEAAALFGEMFEAAFANPAKFSA
                     VPLAKQWHLVRVANRPLAHLCFSPHTSVNLSLDPVGAAIQQASSSVLFSIAFLNQIKS
                     GPTREAIDALMEKNVFSYGIADKTGGLNVHKPDGSVATVNFAYLAANAPEPFKSEWSG
                     GAGIHIHHKFVVTDFSLPSAKVFTGSSNLSETAETKNGDNLVMIEDSRVATAYAIEAV
                     RMFDHLQFRTLMQAKAGAKSKAKAKSAKAKKPAAKGSHAALTLRLPEKIGGKPAWFAD
                     YYKEDSQAERDRKLFGG"
BASE COUNT          819 a         1404 c         1387 g          834 t
ORIGIN      
        1 ccaggaatca gacatccgcc cgatgtgaac acctggattc ccttcccctc gcgatactga
       61 cgtatcactc ggccgggaat gacaacggag atttgcggtg gttgcgtcct acgccgcggc
      121 tttcgccgcc agggcgcaga gcagggccag cgtttcgggt atcacccagc cgttcgcttc
      181 cagcgtgcgc ccttcggcga cgtcttcggc catgacctgc gccagaggac ggtcgccgat
      241 ccgctcgcgc aggtcgtcgg caacgatcct ggcgtcgaaa tcgtcgaggt cggccagcac
      301 cgcttcgccg atctgcgcgt cggcgtcgcg gtcgtccagc agcgtcagca gatcgtcata
      361 ggggctgggc gcatagcgct tgcgcagcag gcgccttcct tccaacgcga cggccgacag
      421 cgcgaccgcg ccgcccgcgc cgatcaggat attgcgccgg gtgagcagtt cattcatcgc
      481 cgcaggccct tcagatggtc cgccaggcgc agcgccagcg ccagcaggtt cagcgtcggg
      541 ttcaccgcgc tgtaggtcgg ataaaccgag cttccggcga cgaacaggtt ggcgacgccg
      601 tggacctgca gattggcgtc gaccacgccc ttgtgcggat cggcgtccat gcgcgtcgtg
      661 cccatgtgat gactggccca atcgagcccg tcgagccact ggctgcgttc cttctggttc
      721 aggcgcagca tgccggtgcg cgacgccagc agttggcggc ccatttcctt cagcgtcaag
      781 cggaaacggg cgaaatccga gtccgcgatg cgcatgtaca gcttcagttt cggcatgccc
      841 agggcgtcgc gctcgctgtc cagtattaat cggcggttcg ggtcgggcgt aacctccatg
      901 ccgcagccga gtgagaaggc cgttgcacta ccggcgttca cgcccagcgc cttggcggtc
      961 gcggccaccg cggctttgcc cagttcgtcc agttccgtct tgttttcgat ggtcgtcgac
     1021 gaatccatga cggcgtggcg gcggcggaat tcctcgctcg ggaacaggcc cgcgcgcgcg
     1081 atggcgccgt gaatcgtctg gttgctctgg tagaagggcg ccagcttgcc gtcgaacagc
     1141 accagcgtga ccgcgtcgcg cgggatcgga tgatcggcaa agaagcggcc gaccaggtcg
     1201 tgggcgttgc ccactccggc cggcatcacg tcgttggagg cgagcatcag ccgcgcgatt
     1261 tcgatcgcgc ccatcgccag cacggtgatc ttcggcttca ccttgaattt ccggccggtc
     1321 agggtggcga cgtcgagttc gccgatcctg gcggcgctgc cgtccagtcc caggcgggtg
     1381 acgttggcgt gcagataggt ggagagccgc gcaatgcgct tcaggtcttc ggcatagcgg
     1441 tcgccgaaat gggtgggcag cacgctgccg cgcatcttgc tgaactggaa ccagcgggtg
     1501 gtgaggccgc cttcgccgag tgctagcagg ggtgcggggg cccattcctt gatgcggtcg
     1561 taaaggaagg ggccggcctc gcacagcgac tgggcgcgcg cgtaataggg atcgagggcc
     1621 tcgcgtccga acggccagcc cgaatagggc atccaggtgc gttcctcgaa gtcgatttta
     1681 tccatcggcc ggcaccagcc gccccaatgg ttcgtgctgc cgccgagata gcgcagccgc
     1741 gtcgcgtcca gcgtcaggta cggcgcgccg accttggagc cttcgtacag cgcctgggtc
     1801 ttggcgtcga aggtcatgcc gccgctttcc agcagcacca tgcgcagcgg cgtggcggcc
     1861 agcgtcagcg ccagcgaaat cccgaccgga ccgccgccga cgatcgccag atcggtttcc
     1921 acaacggtgc cctcggggac ggtgcgggcg tcgatgaatg cggccaatgc gctcgccttt
     1981 cgaagaattc atccggcagc gtgcgaccat gctagcggtg cccgcgtggt cttgtcttgt
     2041 cggaattgtc gcggcggcac aagccggaat ttaccgcacc ccggatttcg cgaacggccc
     2101 ggacgcgaag cctgaatgac cgtgacttcc acggaaggcc ttcggcccgc ccgcgatgga
     2161 caaatccctt cactagtgtc gtggtttcga agttcgcacc atgcgcacta tcgggctgca
     2221 agcgaacttc gaaaccaaaa cgacactaga gcctttcatc gtttcacgga aacgctgaaa
     2281 ggctctaagt tcttaatttg tcgcgttttc ggacggaaaa ccgcccggcg cgccttgcgc
     2341 gcctattggt tccatagcgc gctttcgcgc gggcactttt cctgaaaacg ctctaggatt
     2401 caataggttg ctagtgtcct tcgattccga aattcgcccg aagccgatat agagagggtg
     2461 cgaatttcgg aatcgggaca ctagtatcaa tttataattg aatccgggag ggcttgtcat
     2521 gtccgatttt tccaccgagg cggcaaagga cggtctgaaa ctgaagctgt ggcggggcga
     2581 gcgcatgtgc cttgtcggca tgaacgtcga ggcgccggag ccggattttg tcggcttcgc
     2641 catcgaggtg aaacggcccg gcgcgggcgg cttcatgccg ctgcgcaacc ggctggcctt
     2701 ttcctatccg cagacgccgg acgcctccgt gaccggagcg cggcaattct cctcgctgga
     2761 agcgccgttc cagaaattcc gctggattca ttttccgcag gagccgcagt tgggcagcta
     2821 cacctatcgc gtcaccaagc tgcacatgcc gggcgagggc aggctggtcg cgggcacccg
     2881 gcttgccgcc ggcatatcgc tcggacccgt cacctatgac ggctatctcg acatcggctt
     2941 cacccgcaac ttcgcctcgt cgcagattta tgcggagcgc tacaagaaca atcccaatat
     3001 cattcccacc aaggccgacg gcggcctgga attcaagaag gtcgcgggcg acgtttatcg
     3061 ctggctcggt ttcgaagcct atcagatgat tttcgatttt ctggacgagg cggtgaagga
     3121 ttcctccgtc tcgctcgacg tgctggccta cgacctcaac gagccggaca tggtcgcggc
     3181 gctggagaaa ctaggtcgcc ggctgcgcat cgtcatcgac gattcgaaaa cccattccgc
     3241 caagacgacg gcggaatcga aggcggcggc ccgcttggtc aagtcggccg gggcgcagaa
     3301 cgtccgccgc acccatttca agagcctgca acatcacaag gtgctgatcg ccaaacgcgg
     3361 cggcaagccg ttcaaggcgc tgttcggctc gaccaatttc agcttccgcg gcttatacat
     3421 ccaggccaac aatgccttgc gcttcgacgc gccggaggcc gccgctttgt tcggcgagat
     3481 gttcgaggcg gcgttcgcca atcccgccaa gttcagcgcg gtcccgctcg ccaagcaatg
     3541 gcatctggtc agggtggcga accggccgct ggcccatctg tgcttttcgc cccacacgtc
     3601 ggtgaacctg tcgctcgatc cggtcggcgc ggcgatccag caggcgtcgt cctccgtcct
     3661 gttctccatc gccttcctca accagatcaa aagcggtccg acccgcgaag ccatcgacgc
     3721 gctgatggaa aagaacgtgt tcagctacgg catcgccgac aagacgggcg gcctcaacgt
     3781 ccataagccg gacggttccg tcgccacggt gaatttcgcc tatcttgcgg ccaacgcgcc
     3841 cgagccgttc aagagcgaat ggtcgggcgg ggcgggcatc cacatccacc acaagttcgt
     3901 ggtcacggat ttcagcctgc cgtcggccaa ggtgtttacg ggctcctcga atctctccga
     3961 aaccgccgag acgaaaaacg gcgacaatct ggtgatgatc gaggattcgc gggtggccac
     4021 ggcgtacgcc atcgaagcgg tccgcatgtt cgaccatctg caattccgca ccctgatgca
     4081 ggccaaggcg ggcgccaaat cgaaggcgaa ggcaaaaagc gcgaaggcca aaaagcccgc
     4141 ggcgaaaggc agccacgccg cactcacctt gcgcctgccg gagaagatcg gcggcaaacc
     4201 cgcctggttc gcggattact acaaggaaga cagccaggcg gaacgggacc ggaaattgtt
     4261 tggggggtga ctagtgtccc gattccgaaa ttcgcccgaa accaatatag agatggtgcg
     4321 aatttcggaa tcgaaaggac actagcaacc tattgaatcc tagtgtcgtt ttggtttcga
     4381 agttcgcttg cagcccgata gtgcgcatgc tgcgaacttc gaaaccacga cactagagcc
     4441 gctt
//
LOCUS       DASWPU010000019         4522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_73582,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000019 DASWPU010000000
VERSION     DASWPU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73582"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="VGC27_00385"
     CDS             <1..1453
                     /locus_tag="VGC27_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281067.1"
                     /translation="TDWNSLISANEVSIASAAACDLDAAGSLFVQITGNTTIASFGTG
                     TNKLRFVRFAQALTLTHNAASLILLGAANRITAANDVGIYASDANGNWRERSYFVAAA
                     APGSGGGSGGTLASKAEAEAGTDNTAVMTPQRTSQAILANALGFRNIAGRNGGFEVWQ
                     RLGGTGGATFIAVPAGTTAYTCDGWYLKTGATQASAVSRIAGLTGGSRYAAQVQRSAV
                     QTGTGAIYFAMPLDIDELKKAAGKALQFFFTVSTGANWSPANGTLTYNVYFGTGAAGK
                     RNGAPYAGETNPVTGSVNLAQGASAAQVIAAISPAVAANVACAEIQFAWTPSGTAGAN
                     DWFAIDDVGLHAVPAGLPASAPAFERTEYVWDLQRCCVHYYRRYATVANETIGMMTAY
                     TTTQLWGTVLQLPVSMRSTPNVAVSAPGDLGTFGAGSGTTAIASTNFSGSSAQTISSY
                     NGIFAANAILTIGQAAILTFRTTTGWIEASAEI"
     gene            1488..1904
                     /locus_tag="VGC27_00390"
     CDS             1488..1904
                     /locus_tag="VGC27_00390"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEY0281068.1"
                     /translation="MQKVLTLDAPWDASRWPHFTAREIACPCCGEVTVWPLALDALEA
                     LRAAMNAPLRINSGHRCALHNARVGGAPLSLHKRLAFDVALARHDPAALASAARACGF
                     RGFGFGQTFLHLDTRPRLARWFYGERSKEKWTSVLS"
     gene            1883..2362
                     /locus_tag="VGC27_00395"
     CDS             1883..2362
                     /locus_tag="VGC27_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281069.1"
                     /translation="MDVGSLLGTGASALGGGLFGLVGNVASKVIGIFEAKQQFAQKKE
                     EWSHEERLLDMQTKAKAAETEQELAVTASSGSWSGLSESLKAETSIGSTYPWVNAVRA
                     LVRPALTLGLASFLCAAFFAMAPGDIDRAYVADSLVFAAVTAIVWWFGDRAPKKARS"
     gene            complement(2369..3094)
                     /locus_tag="VGC27_00400"
     CDS             complement(2369..3094)
                     /locus_tag="VGC27_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281070.1"
                     /translation="MLTCRGFGILFSAACLTVLAAAQAGAEPALPAPFQKYLDSPAFA
                     SRLSDALAMAKTTHFADCGTFKASAPRIVPLSQITLGDDGLPKSGQWKVSAAAEGCGK
                     ARIVNFFFVVGKDDKVFFVQAIPGTTRGDFRLQSDTVMYVLMAAVKQTKDCKGASISD
                     TRFDSEDPLHDGKSAGGIALQFQKSWHETWTIDGCGHFYDVRVDYLLGPAGTTIMASD
                     KNVTERPSPDAKPSDAAVQPAPK"
     gene            complement(3287..3667)
                     /locus_tag="VGC27_00405"
     CDS             complement(3287..3667)
                     /locus_tag="VGC27_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281071.1"
                     /translation="MKLKKLLLTVAAAAVLSSAGAGMALAAPHDRGGHHDSNRGGDRR
                     DGYRHDSNRVGHDRYWRKEYRHGYVDHKRVFVNLRKHHYTRFVGNPYWYQGRYVVRSY
                     DRVGRVVFVEVNPYTGGFVGVIRF"
     gene            3813..4430
                     /locus_tag="VGC27_00410"
     CDS             3813..4430
                     /locus_tag="VGC27_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281072.1"
                     /translation="MRARVISFFFLLLTLASPALAADRQAEGRPSFDRILPEIRRHTP
                     GTFYDAEGPYIGRDGQTRYRIKWMTPDGRVVWFDADARTGRRLAPLPYPGSRYDGGPS
                     GSTPSGGGGYGESRRETGRHGDNRDNRDNRDNRDGSDRYDRGHRGNYRDNYRDNDDSN
                     RFRNWPGYDRGQGDGTRDRGGWQRGGSDRGSSGRGGSRHGRGGRD"
BASE COUNT          780 a         1471 c         1468 g          803 t
ORIGIN      
        1 gacggattgg aattcgctga tttcggcgaa cgaggtttcc atcgcctcgg cggcggcctg
       61 cgatttggac gcggcgggat cgctgttcgt gcagattacc ggcaacacca cgatcgcctc
      121 gttcggaacc ggaaccaaca agctgcgctt cgtgcgcttc gcccaggcgc tgacgctcac
      181 ccacaacgcc gcgtcgctga tcctgctggg tgccgcgaac cgcatcacgg cggcgaacga
      241 tgtcggaatc tacgcttcgg acgcgaatgg aaactggcgc gagcgatcct atttcgtcgc
      301 cgcggcggct ccgggaagcg gcggcggaag cggcggaacg ctcgccagca aagccgaggc
      361 cgaggcgggc accgacaaca cggccgtcat gacgcctcag cgaacctcgc aggccatcct
      421 cgccaatgcg ctcggcttca ggaacatcgc gggccgcaac ggcggtttcg aggtgtggca
      481 gcgattgggc ggcacgggcg gggcaacctt catcgcggtc ccggcgggca cgacagccta
      541 cacctgcgat ggctggtatc tgaaaaccgg cgccacccag gcgagcgccg tcagccggat
      601 tgccggcctc actggcggct cgcgttatgc ggcgcaggtg cagcgcagcg cggtgcagac
      661 cggcaccggc gcgatctatt tcgcgatgcc gctcgacatc gacgaactca agaaggccgc
      721 cggcaaagcc ctgcaattct tcttcacggt ttcgaccggc gcgaactggt cgccggcgaa
      781 cggcacgctg acctataacg tctatttcgg cacgggggcg gccggcaagc gcaacggcgc
      841 accctacgcc ggggaaacca atcccgtcac cggctcggtc aatctcgcgc aaggggcctc
      901 cgcggcccag gtgattgcgg cgatctcgcc ggccgtcgcc gccaatgtgg cctgcgcgga
      961 gatccaattc gcctggacgc cgtcgggcac ggccggggcg aacgactggt tcgccataga
     1021 cgatgtcggc ttgcacgccg taccggcggg gcttccggcg tccgctcccg ccttcgagcg
     1081 gacggaatat gtgtgggatt tgcagcgctg ctgcgttcac tactatcgcc gctacgcgac
     1141 ggtcgcgaac gagaccatcg gcatgatgac ggcctataca acgacccaac tttggggaac
     1201 cgtcttgcag cttcccgtgt cgatgcggtc cacgccgaac gttgccgttt ctgcgcccgg
     1261 cgatctcgga acattcggcg caggcagtgg aactacggcg atagcgagca caaattttag
     1321 cgggtcgagc gcacagacga tatcgagcta taacgggatt ttcgccgcaa acgccatcct
     1381 cacaatcgga caggccgcca tcctcacctt ccgaaccacg accggctgga tcgaagccag
     1441 cgcggagatt tgagcggcgg caggcgcgcg ctttgcggca gactttgatg cagaaggtcc
     1501 ttaccctcga cgcgccatgg gatgcttccc gttggccgca cttcacggcg cgcgagatcg
     1561 cctgcccatg ctgcggcgag gtcaccgtct ggcctctggc gctcgacgcg ctcgaagcct
     1621 tgcgcgcggc gatgaacgcg ccgctgcgga tcaattccgg ccatcgctgc gccttgcata
     1681 acgcgcgcgt cggcggcgcg ccgctgtcgc tgcacaagcg gctcgccttc gatgtcgcgc
     1741 tggcgcgcca cgatccggca gccctggcgt cggcggcgcg cgcttgcggt tttcgcgggt
     1801 tcggcttcgg ccagaccttt cttcatctcg atacgcgccc gcgccttgcg cgctggttct
     1861 acggcgaaag gagcaaagaa aaatggacgt cggttctctc ttaggcacgg gcgcttccgc
     1921 gctcggcggc ggcctgttcg gccttgtcgg caatgtcgcc tcgaaggtga tcggcatctt
     1981 cgaagcgaaa cagcaatttg cgcagaagaa agaagagtgg agccacgaag agcgcctgct
     2041 cgacatgcag acgaaggcca aggcggcgga gaccgagcag gagctggccg tcaccgcgtc
     2101 gtccggttcg tggagcgggc tctccgaaag cctgaaggcc gaaacctcca tcggttccac
     2161 ctatccctgg gtcaatgcgg tgcgcgcgct ggtccggccc gccctcaccc tcggcttggc
     2221 ctcgttcctc tgcgccgcct ttttcgccat ggcgccgggc gacatcgacc gcgcctatgt
     2281 ggcggacagc ctggtcttcg ccgccgtcac cgccatcgtc tggtggttcg gcgaccgcgc
     2341 gccgaagaag gcacgcagct aaagcgtttt atttcggcgc cggctgtacc gccgcatcgg
     2401 atggcttggc gtcgggcgac gggcgctccg tgacgttttt gtcggaggcc atgatcgtcg
     2461 tgccggcggg gcccagcaga tagtcgaccc gcacatcgta gaagtggccg cagccgtcta
     2521 tggtccaggt ttcgtgccag ctcttctgga attgcagagc gatgccgccg gcgctcttgc
     2581 cgtcgtggag agggtcttcg gaatcgaagc gcgtgtcgct gatggaagcg cctttgcaat
     2641 ccttggtctg ttttacggcg gccatcaaca catacatcac ggtatcgctt tgcagcctga
     2701 aatcgccgcg cgtggtgccg gggatcgcct gcacgaagaa gactttgtcg tccttgccca
     2761 ccacgaagaa gaaattcacg atccgcgcct tgccgcagcc ttcggccgcg gccgacactt
     2821 tccattgtcc tgattttggc aggccgtcgt ctccgagcgt gatctgcgac aacggaacga
     2881 tccgcggcgc ggaggcctta aaagttccgc agtcggcgaa atgggtcgtc tttgccatcg
     2941 ccagcgcgtc cgacagacgc gaggcgaagg ccggcgagtc gagatatttc tggaacggtg
     3001 cggggagcgc cggctctgca ccggcttgtg ccgcggcgag aaccgtaagg caggccgccg
     3061 aaaacaggat tccaaatccg cgacacgtca gcatggcaag cccccagcct gaaatcgttc
     3121 taaaggttgc gcgtattcta gcacatcgcg ccggcggcgt ttctctgctt tgcagccccc
     3181 caacgccgaa agcccgcgag aggtctcgcg ggctttcgct tgatcatcgt gcggctctac
     3241 ggggaggggc acaccgcgag cggcccgact caagttacga aaagcatcag aaccggatca
     3301 cgccgacgaa gccgccggtg taggggttca cctccacgaa cacgacgcgg ccgacgcggt
     3361 cgtagctgcg caccacgtaa cggccttgat accagtacgg attgccgacg aagcgggtgt
     3421 agtgatgctt gcgcagattg acgaacaccc gtttgtggtc gacgtaaccg tgacggtatt
     3481 ctttgcgcca gtagcggtca tggccgacgc gattgctgtc gtgacgatag ccgtcgcgcc
     3541 ggtcgccgcc gcgattgctg tcgtgatgtc cgccgcgatc gtgaggggca gccagggcca
     3601 tgccggcgcc ggccgaggac agaaccgccg cggcggcgac tgtcagaaga agtttcttga
     3661 gcttcattgt catctccttt gggctcttgc tgcggaaggc ggttgatcga atcgccgccg
     3721 ttccaatgcg tgcgacatta gggcggcccg cctgaaccag ttctggacgc ggcgttcatg
     3781 gcgggttcat ctgaggcgag gtacaaaacg ccatgcgtgc gcgcgttatt tccttctttt
     3841 ttctgctgct tacgctcgcc tccccggccc tcgctgccga ccggcaggcc gagggcaggc
     3901 cgtcgttcga ccggatcctg cccgaaatcc gccgccacac ccccggcacg ttctacgatg
     3961 ccgaagggcc ttacatcggc cgtgacggcc agacgcgcta ccgcatcaaa tggatgacgc
     4021 ccgacgggcg ggtggtctgg ttcgatgcgg acgcgcgcac cgggcgtagg ctggcgcctc
     4081 tcccctatcc gggcagccgc tatgatggcg gcccctccgg cagcaccccc tccggcggcg
     4141 gcggttacgg cgagagccgt cgcgaaaccg gccgtcacgg cgacaatcgt gacaaccgtg
     4201 acaaccgtga caaccgcgac ggcagcgacc gttacgaccg cggccatcgc ggcaactacc
     4261 gcgataacta tcgcgacaac gacgattcca atcgcttccg taactggccg gggtatgatc
     4321 gcggccaagg cgatggcacc cgggaccgcg gcggctggca gcgtggcggt tcggaccgcg
     4381 gcagttccgg ccggggcggt tcccggcacg gtcgcggcgg cagggattga taaagcgatg
     4441 cgcatcctgc ttgtcgaaga tgatgtcgat ctgcaaaggc tgctgaagaa gtcgctcggc
     4501 gatgccggtt atgtcgtcga ta
//
LOCUS       DASWPU010000020         3078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_24616,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000020 DASWPU010000000
VERSION     DASWPU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24616"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1980
                     /locus_tag="VGC27_00415"
     CDS             112..1980
                     /locus_tag="VGC27_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HEY0281073.1"
                     /translation="MAGVTAPAYAELSRGEAHVWEMQEIILQSQRDYANPYADVECWI
                     DLKGPGFDKRVYGFWDGGRTFKVRFVATAPGEWRWSTGSNQPTDTGLNGGTGVLRAVA
                     WTDAEKAANANRHGFVRATGHALQYADGTPFFLTGDTWLAASTWRLPWRGGAEPDAAY
                     VPGSGISFEEAVAYRKRQGFNSTSFIAAFPNWAADGNGATFADAKGVFLRNAWEKFGT
                     WAPNATVTTADGATTTAKDMQDEAGNLAFATVPDHDGMADFDRLNPAYFQSLDRKMRT
                     LAEQGFVPVLEPVRRDTGPSWKAYFDFNTSYARYVQYLAARYGAYNLIFSGIHLDWIP
                     KEYSLTAGEYNTALTGWWKTYGALPFGQPVTVLIDSSTYTRFGHGAQAPWLTMQSVGN
                     MPRHHGIYPMIEEIFRLNPAYPVANLEPYYAGWNHTMNRPGGETPEPGSERDTYFARA
                     MMYGSVLSGGLAGHVYGSGAYDVTTTGEPSGWRPYIWQALRYESGAQMQYLRKFMLSE
                     GARYQQLILATDDLRPRATPAAPADGLDGWSFMMRTADRSLAFLYFENRAVRPQMNGF
                     SPHAHYRWSWFDPANGSWSPAVTLAANAQGVLAAPAFPGGRVQANRDWAAKLVAAH"
     gene            2140..2874
                     /gene="rquA"
                     /locus_tag="VGC27_00420"
     CDS             2140..2874
                     /gene="rquA"
                     /locus_tag="VGC27_00420"
                     /inference="COORDINATES: protein motif:HMM:NF038261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodoquinone biosynthesis methyltransferase
                     RquA"
                     /protein_id="HEY0281074.1"
                     /translation="MFQSIDETGPLQRVEGDTLSAGDIRIPDYLRRHYWWAYIHPRGV
                     KFFDRRWIVNLILLGNYNRLRDRALAEFGKAASGNILQLACVYGDLTTQLEKRVAAGS
                     GSLDVIDIVPIQLSNLKKKLPGLTRTRLLRMDSADLALPDASYDRVMLFFLLHEQPAS
                     HRTRTLREAFRVVRPGGKIMIVDFARPHWWNPLFYLWRPLLAVLEPFALDLWHSDIAS
                     LLPVRWNKLRRETFFGGFFQKVVVTR"
     gene            2899..>3078
                     /locus_tag="VGC27_00425"
     CDS             2899..>3078
                     /locus_tag="VGC27_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281075.1"
                     /translation="MQPDIADGANFKTTTLGCGRGRGLFSPPKKAEDRAFPAKGIAMK
                     QTRRAFLATAAALSAA"
BASE COUNT          551 a         1001 c          948 g          578 t
ORIGIN      
        1 gcgtacggac gggcgaagac gggctctcgc ggcccaagtt gcgacggcat tggcggcgtc
       61 ttatggtcgg cggccatgcg atatctcttt cgaataattc tcgcgctctt tttggccggg
      121 gttaccgcgc cggcttatgc cgaactgtcg cgcggcgagg cccatgtctg ggaaatgcag
      181 gagatcatcc tgcaaagcca gcgcgactac gccaatcctt atgccgatgt cgaatgctgg
      241 atcgacctga aagggccagg cttcgacaag cgcgtttacg gcttctggga cggcgggcgc
      301 acgttcaaag tacgcttcgt ggccaccgct ccaggcgaat ggcgctggag cacgggttcc
      361 aatcagccaa ccgacacggg cctcaacggc ggcacgggcg ttttgcgcgc tgtggcctgg
      421 acggacgcgg aaaaagcggc caacgccaac cgccacggct tcgtgcgcgc gacgggccac
      481 gcgctgcaat atgccgacgg cacgccgttt ttcctgacgg gcgacacctg gctggccgct
      541 tcgacctggc gcctgccctg gcgcggtggc gccgagccgg atgccgctta cgttcccggc
      601 tccggcatca gcttcgagga ggcggtcgcc tatcgcaagc ggcagggctt caactcgacc
      661 agtttcatcg ccgcgtttcc caactgggcg gcggatggaa acggcgccac ctttgccgac
      721 gcgaagggcg tctttctgcg caacgcgtgg gaaaagttcg gcacctgggc gccgaatgcc
      781 acggttacca ccgccgacgg cgccaccacg accgcgaagg acatgcagga cgaagcgggc
      841 aacctcgcct tcgcgaccgt tcccgatcat gacggcatgg cggatttcga ccggctcaac
      901 ccggcctatt tccagagcct cgaccgcaag atgcgcaccc tggccgagca aggcttcgtg
      961 cccgtgctcg aaccggtgcg gcgcgatacc ggcccgtcgt ggaaggccta tttcgatttc
     1021 aacacatcct acgcccgcta tgtccaatat ctcgcggccc gctacggggc gtataatctg
     1081 atcttcagcg gcatccatct cgactggatt ccgaaggaat acagtttgac cgccggcgag
     1141 tacaacaccg ccctgaccgg ctggtggaaa acctacggag cgctgccttt cggccagccc
     1201 gtcacggtgc tgatcgacag ttcgacctac acccgcttcg ggcacggcgc gcaggcgccc
     1261 tggctcacca tgcagtcggt gggcaacatg ccgcgccacc acggcatcta tccgatgatc
     1321 gaagagattt ttcggctgaa cccggcttat ccggtggcca atctcgaacc ttattacgcc
     1381 ggctggaatc acacgatgaa ccggcccggc ggcgaaacgc ccgagccggg ttcggagcgg
     1441 gacacgtatt tcgcgcgcgc catgatgtac ggctcggtgc tgtcgggcgg cttggccggt
     1501 catgtctatg gcagcggcgc ttatgacgtg accacgacgg gcgagccaag cggctggcgc
     1561 ccttatatct ggcaagcgct gcgctacgaa tccggcgctc agatgcagta tctgcgcaag
     1621 ttcatgctgt cggaaggcgc gcgctatcaa cagctcatcc ttgccaccga cgatctgcgc
     1681 ccgcgcgcga ccccagccgc gcctgccgac ggactggacg gctggtcgtt catgatgcgc
     1741 acggcagacc gcagccttgc cttcctttat ttcgaaaatc gcgccgtgcg tccgcagatg
     1801 aacggatttt caccgcacgc ccattatcgc tggtcgtggt tcgatccggc caatggaagc
     1861 tggagccctg ccgtcacgct ggccgcgaat gcgcaaggcg tgctcgcggc gcccgccttt
     1921 ccgggaggcc gggtgcaggc taaccgcgat tgggcggcca agctcgtcgc ggcgcactga
     1981 agcaggcaca agtacgatcg atccctaagc ggccaatcca gcccttgagg aaagacggcc
     2041 tgccaccttt cgccgcacga cggaaatccg tcgcattgcc caggcgcatg gaaggtgcat
     2101 cctgaatgcg aggcagtttt tcacaaatca gcgggccgca tgtttcagag catcgacgag
     2161 actggcccgt tgcaacgggt cgagggggac acgttgtccg ccggcgacat ccgtattccg
     2221 gattacctgc gccgccatta ctggtgggcc tatatccatc cgagaggcgt gaagtttttc
     2281 gaccgccgct ggatcgtcaa ccttatcctg ctgggcaatt acaaccggct gcgcgaccgc
     2341 gcgctggcgg aattcggcaa ggcggcatcg ggcaacatcc tgcaactggc ctgcgtttac
     2401 ggcgacttga ccacgcagct cgaaaagcgc gtcgccgcgg gcagcggctc gctcgatgtc
     2461 atcgatatcg tgccgatcca gttgagcaat ttgaagaaga agcttcccgg ccttacgcgc
     2521 acgcgcctgc tgcgcatgga ttccgccgac ctcgcgctgc ccgatgcaag ctacgaccgg
     2581 gtcatgctgt tcttcctatt gcacgagcag ccggccagcc atcgcactcg gacgctgcgc
     2641 gaagcgttcc gcgtggtcag gccgggcggc aaaatcatga tcgtggattt tgcccggccg
     2701 cactggtgga acccgctgtt ctatctgtgg cggccgctgc tggccgtgct cgaaccgttc
     2761 gcgctcgatt tgtggcatag cgacatcgcc tccttgctgc cggtcagatg gaacaagctg
     2821 cgccgggaaa cttttttcgg cggcttcttc cagaaagtgg tcgtcacgcg ctagtgtcgt
     2881 tttagtttcg aagttcgctt gcagcccgat atcgcggatg gtgcgaactt caaaaccacg
     2941 acactaggct gcggccgtgg tcgcgggttg ttctcgcctc cgaaaaaggc ggaagatcgc
     3001 gcgttcccgg ccaaaggtat cgccatgaaa cagacccgcc gcgcctttct cgccaccgct
     3061 gctgcgctat cggccgcc
//
LOCUS       DASWPU010000021         5210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_61381,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000021 DASWPU010000000
VERSION     DASWPU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61381"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..684
                     /locus_tag="VGC27_00430"
                     /pseudo
     CDS             34..684
                     /locus_tag="VGC27_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
     gene            complement(721..2133)
                     /locus_tag="VGC27_00435"
     CDS             complement(721..2133)
                     /locus_tag="VGC27_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEY0281076.1"
                     /translation="MSTETAVKALDAFQSKNKSDTVFIGHPRGLGWLSASEYWERFSY
                     YGMQTLLVLYLIHYLLLPGNIEQVWGFDAFRRLILWLYGTQSQMAVASNTAQLYAALV
                     YVTPLAGGYLADRVIGRTKTVTIGAVLMVIGTFLLALNQTFLLGLAFLLAGVGCFKGN
                     IASQVGDLYTIDDPRRADGFQIYFLGIQLAVILSPIICGYLGQRVDWHLGFVAAGVGM
                     VLGLTIYLSGRRTFPPEPLRKTGTEVKRPPLTGRDLRVVILLVALLPVLALSIIGNQE
                     IFDAYLVWGEKNFQTVFFGFNMPITWIVSLDAFVSTTTMVAVIAFWRWYGKRWREPNE
                     ITKMIIGVAIGALGPLVLAGSAAVATATHHPVPLYWALGFHILNDIGFANVLPVGLAL
                     YSRAAPKGLGGTMIAVYYVHLFMGNLLVGYVGGLLGSMPDTEFWLLHVGLMAVSVVLL
                     LAARFFFGHLLAPTNEQTAA"
     gene            complement(2266..2847)
                     /locus_tag="VGC27_00440"
     CDS             complement(2266..2847)
                     /locus_tag="VGC27_00440"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0281077.1"
                     /translation="MAKSDISRPPRADAQRNRQRLLATALKLFTSSKEEVTLSAVAEH
                     AGVGIGTLYRHFPTRDALVEAIYRQEVKRLSAAAPALLKGMPADKALEEWLTRYAGLV
                     AAKRGLKEAVRSIFEPGADTYAYSRDRMTSAVTLLLDAAAKSGAIRSDYDPQDVLLAV
                     AASTWTFVNDGDWQERARRVLRLVMDGLRYKPR"
     gene            2943..3788
                     /locus_tag="VGC27_00445"
     CDS             2943..3788
                     /locus_tag="VGC27_00445"
                     /inference="COORDINATES: protein motif:HMM:NF006114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0281078.1"
                     /translation="MPQSLSYKVWFITGASKGFGYIWTEAALARGDSVAAAARNIGSL
                     DGLKAKYGDRLLPLTLDVGDKAAVDAAVEKAHKQFGRIDIAINNAGYGLFGAIEEVSE
                     QEARAQIETNLFGALWVTQAVLPIMRAQASGHIIQISSIGGVSAFPTIGLYHASKWGL
                     EGFSQALASEVKGLGIKVTIIEPGGFATEWGTTSAHRAKQLPAYDAARAAIASFRTAA
                     TPGDPKATASALLRIVDAAEPPLRVFFGDGGLPMAKREYAERIATWEKWNDVSVEAQG
                     SLAAH"
     gene            3814..4776
                     /locus_tag="VGC27_00450"
     CDS             3814..4776
                     /locus_tag="VGC27_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0281079.1"
                     /translation="MSKVFGTQSTTDDVLEGIDLAGKRVLVTGVSAGLGVETARALAA
                     HGAQVVGAVRDLDKARGATKAVRDQAQNGGGFELVELDLASLASVRASADKLLAAGKL
                     FDAVIANAGVMACPKSTTVDGFETQFGTNHLGHFVLVNRIASLMKRGSRLVNLSSAGH
                     RYSDVDLDDPNFERTPYTEFGAYGRSKTANILFAVEFDRRHKANGIRATAVHPGGIKT
                     ELSRHMTPEATQALIDSINANAKAGTPAFTFKTVPQGAATTVWAGFFAPADLVGGKYC
                     EDCRVAEIIEDAAIRSGVRPYALDPERAKALWAKSEEMVGERFS"
     gene            complement(4787..>5210)
                     /locus_tag="VGC27_00455"
     CDS             complement(4787..>5210)
                     /locus_tag="VGC27_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY0281080.1"
                     /translation="GEFLEHATVFGHRYGTPREPVLEALTAGRDVLFDIDWQGTQQLR
                     QQVREDVVTIFVLPPSREELERRLHTRAQDSAEVVAKRMAKANDEISHWAEYDYVIVN
                     EDIARARAKVETILEAERLKRARQPGIPAFVESLTGGE"
BASE COUNT         1006 a         1665 c         1665 g          874 t
ORIGIN      
        1 gatgtagccg aggcttatcc gggacggctt cagattgtcc ccgccgatgc gctggcactc
       61 gacgaagcaa agctgttgcg cgaatgcggg gtgggaggtc cggtccgcgt cgccgccaat
      121 cttccttaca acatcggcac ggcgctgttg gtgaagtggt tgacgggcgg agcatcggcc
      181 agcaaaagcg ggagcctcgg cgaaccggtt ttgcgtccgg ccgtgcgacc caaagatacc
      241 tggccgccgt tctggagcag cttgacgctg atgtttcagc gcgaggtggc cgagcgcatc
      301 atcgccgcgc cgggcgcgca tgcctatggc cgcctgtcgg tgctggcgta gtggcgcgcg
      361 caggcgaaaa tcctgttcga cgtttcgcga agcgccttcg tgccgccgcc gaaagtgact
      421 tcggccgtca ttcgcgtcga gccgcttgcc gagcctgtcg cgccggcgcg cctcgccgat
      481 ctcgaagcgg tgacggccgc cgccttcggc cagcgccgca agatgctgcg ccagagtttg
      541 aagacgctga cgcccgatgc ggaagcgctg ctgcgcgacg ccggcatcga tccgaccgaa
      601 cggcccgaac aactctccgt ggcgcaattc gcggccctgg cgcgggcttg ggcgaaacat
      661 gttgataacc aaggcggcgc atgaaagagc gacggctgca atcttgaatc gaagcgagcc
      721 tcacgccgcc gtttgctcat tcgtcggggc cagcagatgg ccgaagaaga aacgagccgc
      781 aagcagcagc accacggaaa ccgccatcaa tcccacatgc agcagccaga actcagtgtc
      841 cggcatggag ccgagcagtc cgcccacata acccaccagc aggttgccca tgaaaaggtg
      901 gacgtaatag acggcgatca tcgtgccgcc caatcccttc ggcgccgcgc gcgaatagag
      961 cgccagcccc accggcagaa cattggcgaa gccgatatcg ttgagaatgt ggaatcccag
     1021 cgcccagtac agcggcacgg gatgatgcgt ggcggtcgcc accgcggcgc tccccgccag
     1081 caccagcggc cccagcgcgc cgatcgcgac gccgatgatc atcttggtga tctcgttcgg
     1141 ctccctccag cgcttgccat accagcgcca aaaggcgatc accgcgacca tcgtcgtggt
     1201 gctgacgaat gcatcaagcg acacgatcca ggtgatcggc atattgaagc cgaaaaacac
     1261 cgtctggaaa ttcttctctc cccacaccag ataagcgtcg aagatttcct ggttgccgat
     1321 gatcgacagc gcgagcacgg gaagcagcgc caccaagagg atcaccaccc gcaagtcgcg
     1381 gccggtcaga ggcggacgct tcacttcggt gccggttttg cgcagcggtt cgggcgggaa
     1441 cgtgcggcgg ccggaaagat agatcgtcag ccccagcacc atgccgacac ccgcggcgac
     1501 gaaacccaga tgccagtcca ccctctggcc gagatagccg cagatgatcg gcgagaggat
     1561 cacggcaagc tggatgccga gaaaatagat ctggaatccg tcggcgcggc gcggatcgtc
     1621 gatggtatag agatcgccca cttggctcgc gatgttgccc ttgaagcagc ccacgccggc
     1681 gagaaggaac gccagcccca agaggaaggt ctggttcagc gccaaaagga aagtgccgat
     1741 caccatcaga accgccccga tggtcaccgt cttggtgcgg ccgatcacgc ggtcggcgag
     1801 gtagccgccg gcaagcggcg tgacatagac aagggccgcg tagagttgcg cggtgttgga
     1861 ggcgaccgcc atctgcgatt gtgtgccata gagccacaag atcagccggc ggaacgcgtc
     1921 gaacccccag acctgctcga tgttaccggg cagaagcagg tagtgaatga gatagagcac
     1981 caggagcgtc tgcatgccgt aataggagaa acgctcccag tattcggacg cagaaagcca
     2041 ccccagcccc ctcggatggc cgatgaacac ggtgtcggat ttgtttttcg attggaaagc
     2101 atcaagcgcc ttgacggcgg tttctgtgct caagctgcgt tccccatttg acgcgcaagc
     2161 actgtcgcgg agcgacaaac cgattgcaag ccgggcgcgg catgcgtttc catgttcgtg
     2221 cgcggcgcat cgtggactgt gcccgcggct attcccttag ggcaatcagc gcggtttgta
     2281 gcgcaggccg tccatcacga gacgaagcac gcggcgggcg cgctcttgcc aatcgccgtc
     2341 gttcacgaag gtccaggtgc tggccgccac ggccaacagc acatcctgcg gatcgtaatc
     2401 gctgcggatg gcgccgctct tggccgccgc gtcgagcagc aaggtgacgg cgctcgtcat
     2461 gcggtcgcgc gaataggcgt aggtgtcggc gcccggctcg aagatcgacc gcaccgcttc
     2521 cttgagcccg cgcttggccg caaccagacc cgcatagcgc gtcagccatt cctccagcgc
     2581 cttgtccgcc ggcatgccct tcagcagggc cggggccgcg gcgctcaacc gcttgacctc
     2641 ttggcggtag atcgcctcga ccagggcgtc gcgggtcggg aaatgacgat agagcgtgcc
     2701 gatgccgact ccggcgtgtt ccgcgacggc ggaaagcgtc acctcctcct tcgaggaggt
     2761 gaacagtttc aaggcggtag caagcaggcg ctggcggttt ctctgggcat cggcgcgggg
     2821 agggcgggaa atatcggatt ttgccaacgc agcctcttgt aaaacggagg accctccgta
     2881 tataacgcca gaagcggagc atcctcctct tcccctatat agcgtttcgg aggacaaaat
     2941 ccatgcccca atctctttct tacaaagttt ggttcatcac cggcgcctcc aaaggtttcg
     3001 gttatatctg gacggaggcc gcattggcgc gcggcgacag cgtcgccgcc gcggcccgca
     3061 acatcggctc gctcgacggg ctgaaggcga aatacggcga ccggctcctg ccactcacgc
     3121 tcgacgttgg cgataaagcg gcggtcgatg ccgccgtcga gaaggcgcac aagcagttcg
     3181 gccgcatcga tattgcgatc aacaacgcgg gctacggcct gttcggcgcc atcgaagaag
     3241 tcagcgagca ggaagcccgc gcgcagatcg aaaccaacct gttcggcgct ttgtgggtga
     3301 cgcaagccgt gctgcccatc atgcgggcgc aagcctccgg ccacatcatc cagatttcgt
     3361 ccatcggcgg cgtctcggcg tttccgacca tcgggctcta tcatgcttcc aaatgggggc
     3421 tggaaggctt cagccaagcg cttgcaagcg aggtaaaagg tctcggcatc aaggtcacga
     3481 tcatcgagcc gggcggcttc gccaccgaat ggggcacgac ctccgcgcac cgcgccaagc
     3541 aattgccggc ttatgacgcc gcgcgcgccg cgatcgcgag cttccgcact gcggcgacac
     3601 ccggcgatcc gaaggcgacg gcgtccgctt tgctgcgcat cgtcgatgcc gccgagccgc
     3661 cgctgcgcgt gttcttcggc gacggcggat tgccgatggc caagcgcgaa tatgcggagc
     3721 gcatcgccac ctgggaaaag tggaacgacg tatccgtgga agcgcaaggc agtctcgccg
     3781 cgcattgaga cagagcctta gaaaggaaaa aatatgagca aggtatttgg cacgcaatcg
     3841 accaccgacg acgtgctcga aggcatcgac ctcgccggca agcgcgttct ggtgaccggc
     3901 gtgtcggccg gacttggcgt ggagacggcg cgcgcactcg ccgcgcatgg tgcgcaggta
     3961 gtgggcgccg tgcgcgatct cgataaggcg cgcggcgcga ccaaggcggt gcgcgaccag
     4021 gcgcagaatg gaggcggatt cgaactggtc gaactggacc tcgcctcgct ggccagcgtg
     4081 cgcgcctcag ccgacaaact cctcgcggcc ggcaaactct tcgatgccgt catcgccaac
     4141 gcgggcgtaa tggcctgtcc caaaagcacg accgtagacg gcttcgagac ccagttcggc
     4201 accaaccatc ttggccattt cgtgctggtc aaccgcatcg cctcgctgat gaagcgcggg
     4261 tcgcgcctgg tcaatctgtc gtcggcgggc caccgctatt ccgacgtcga tctcgacgat
     4321 ccgaatttcg agcgcacgcc ttacaccgaa ttcggcgcct atgggcgctc caagacggcc
     4381 aatatcctgt tcgcggtcga gttcgaccgg cggcacaagg cgaacggcat tcgcgccacc
     4441 gccgtgcatc cgggtggtat caagaccgaa ctcagccggc acatgacgcc ggaggccacg
     4501 caagcgctga tcgattccat caacgccaac gccaaggcgg gcacgccggc cttcaccttt
     4561 aagacggtgc cgcagggcgc cgccacgacg gtgtgggccg gatttttcgc gccggccgat
     4621 ctggtcggag ggaagtattg cgaggattgc cgcgttgctg aaatcatcga agacgccgca
     4681 atccgcagcg gcgtgcgtcc ctatgcgctc gatcccgagc gcgccaaggc gctttgggcc
     4741 aagagcgaag agatggtggg cgaacgcttc tcgtaatctt tgaccactac tcgccgccgg
     4801 tcaggctttc gacgaaagcc ggaatgcccg gctggcgggc gcgcttgagc cgctcggcct
     4861 caaggatggt ctcgaccttg gcccgggcgc gggcgatgtc ctcgttgacg atgacatagt
     4921 cgtattcggc ccaatggctg atctcgtcat tggccttggc catgcgcttg gccacgactt
     4981 cggccgaatc ctgggcgcgg gtgtgcagcc ggcgttccag ttcttcgcgc gaaggcggca
     5041 gcacgaagat ggtgaccacg tcttcgcgca cctgctggcg aagctgctgg gtgccttgcc
     5101 agtcgatgtc gaacagcacg tcacgcccgg cggtcagcgc ttccagcacc ggctctctgg
     5161 gcgtgccgta gcgatggccg aacaccgtgg cgtgttcgag gaactcgccc
//
LOCUS       DASWPU010000022         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_91905,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000022 DASWPU010000000
VERSION     DASWPU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91905"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VGC27_00460"
     CDS             <1..465
                     /locus_tag="VGC27_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0281081.1"
                     /translation="RDLARTPIGVAVRSGAAKPDVSTVEAFKQVVLNAKAVAVPGSTS
                     GIFLTKEVFPRLGIADKVNVKVAPRGTGAASMVASGEADVGLLPVSEIMHAPGVELAG
                     VIAEEIQLNQVFAAAVVAGSSEAEAAKRLIAFLASERASAVIKSSGMEPLGK"
     gene            498..872
                     /locus_tag="VGC27_00465"
     CDS             498..872
                     /locus_tag="VGC27_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281082.1"
                     /translation="MEDSNRILEQKALRNVRALVDKLEALEPARPWKPILLLTTVVII
                     AGLVMWVASSRKVAAAADQRQQRSCEFDVWAAKSGEFERGLRQAHPEMPNVDLQKLLE
                     RERPALMAAAQAQCDGRAAKTQ"
     gene            869..1237
                     /locus_tag="VGC27_00470"
     CDS             869..1237
                     /locus_tag="VGC27_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281083.1"
                     /translation="MKEKHRALLMLALVSFSLPLGEMVVLHRPAPRWSTLSFAEAFLT
                     AYAIYWWYVTDKRERSFTAGRVQDFGVILATIIALPVYFVRSRGWWRGALAILGALAI
                     IILVGLLALVGGRIGAAIAF"
     gene            1268..>1552
                     /locus_tag="VGC27_00475"
     CDS             1268..>1552
                     /locus_tag="VGC27_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0281084.1"
                     /translation="MKYQGSCHCGQIAFEVEGQVSEVTSCNCSMCQRKGVLMWFVPRT
                     AMKLLTPPEQMKTYLFNKHKIKHHFCATCGIHPYGEALSPSGEEMAAINAR"
BASE COUNT          296 a          478 c          504 g          274 t
ORIGIN      
        1 agggacctgg cgcgaacacc catcggcgtg gctgtgcgct ccggggccgc gaagcccgac
       61 gtcagcacgg tcgaagcctt caagcaagtt gtgctgaacg cgaaagccgt cgcggtcccg
      121 ggcagcacga gcggcatctt cctcaccaag gaagtgttcc cacggctcgg catcgcggac
      181 aaggttaacg tcaaggtcgc gccgcgcggc acgggcgccg cctccatggt ggcttccgga
      241 gaggcggacg ttgggctcct gccggtcagc gaaatcatgc atgcacccgg tgtcgaactc
      301 gccggcgtga tcgcggagga gatccagctg aatcaggtgt tcgcggcggc ggttgtagct
      361 ggatcgagcg aggctgaagc ggcgaagcgc ctgatcgcgt ttctcgcatc cgaacgcgcg
      421 tcggcagtaa tcaagagcag cgggatggag ccgctcggga agtgaaaccg gaccgccaac
      481 ctcgctagaa tgagccaatg gaagacagca atcgcatcct cgagcaaaag gcccttcgta
      541 acgtccgggc cctggtcgac aagctcgagg cgctcgagcc ggcgcggccc tggaagccga
      601 tcctcctgct gaccaccgtg gtaattattg cgggcttggt gatgtgggtc gcgagctcac
      661 ggaaggtggc cgcggcggca gaccaacgtc aacagcgctc atgcgaattc gacgtttggg
      721 cggcgaagtc aggtgagttc gagcgggggc tccgccaggc gcatcccgag atgcccaacg
      781 tcgacctcca gaaactcctc gaacgcgagc gccctgccct catggcggcg gcacaggcgc
      841 aatgcgatgg ccgggccgcc aagacgcaat gaaggagaag catcgagcgc tcctgatgct
      901 ggcgttggtg tcgttctccc tgccgctcgg tgaaatggtc gtgctccatc gcccggctcc
      961 tcgatggagc acgctctcgt ttgcggaggc attcctgacg gcctatgcaa tctactggtg
     1021 gtacgtcacc gacaagcgcg aacgcagctt caccgctggc agggtccagg acttcggcgt
     1081 catcctcgcc accatcatcg ccctgcccgt gtacttcgtg cgatcccggg gttggtggcg
     1141 cggcgcgttg gcgatcctgg gcgctctcgc catcatcatt ctcgtcgggc tcctcgcctt
     1201 ggtcggcggg agaatcggag cggcaattgc gttctgacgg tagctggcta actcaaggag
     1261 catgcagatg aaataccagg gaagttgcca ttgcggccag atcgccttcg aggtcgaagg
     1321 ccaagtctcc gaagtcacgt cgtgcaattg ctccatgtgc cagcgcaaag gcgtcctgat
     1381 gtggttcgtt ccgaggacgg cgatgaaatt gctcacaccg ccggagcaga tgaagacgta
     1441 cctcttcaac aagcacaaga tcaagcatca cttctgcgcg acctgcggca tccatccgta
     1501 cggcgaagcg ctttctccgt ccggcgagga aatggcggcg atcaatgccc gt
//
LOCUS       DASWPU010000023         3308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_30772,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000023 DASWPU010000000
VERSION     DASWPU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30772"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /gene="hflX"
                     /locus_tag="VGC27_00480"
     CDS             <1..1358
                     /gene="hflX"
                     /locus_tag="VGC27_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY0281085.1"
                     /translation="HVEPKSRKPSDDLDRDASARLAEAVGLTKAIGLEVVAELIVPLA
                     RAVPATLIGSGKVDDIASQVRALEPELVIVNTQLSPVQQRNLEKAWSSKVLDRTALIL
                     EIFGERARTREGRLQVELAHLTWQRSRLVRSWTHLERQRGGFGFLGGPGETQIETDRR
                     LIGERITKIKKELEGVKRTRGLQRKARKRVPFPVIALVGYTNAGKSTLFNALTKAGVL
                     AKDILFATLDPTMRGIRLPGGTKAVLFDTVGFIADLPHELVAAFRATLEEVLEADIIA
                     HVRDAAHAETEAQKADVLAVLAELGVEPRVFPGEVCSGSPSKTRQNIDFRDRPLVEIL
                     NKIDLLEPGARAGLKASAAERPEVPAAVSALTGEGLDDLLRRFESVLTGGNICLRLEL
                     SPSDGEGLAWVYRHGQVLERDDGEAGIVLTLSVHPQDAARFESRFAGKISVEQDIAAD
                     I"
     gene            complement(1453..2280)
                     /gene="mazG"
                     /locus_tag="VGC27_00485"
     CDS             complement(1453..2280)
                     /gene="mazG"
                     /locus_tag="VGC27_00485"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY0281086.1"
                     /translation="MRRLPAFYRGMEKPNPSRDIESILEIMRSLRDPVRGCPWDKEQD
                     FATIAPYTIEEAYEVAGAIEDRDWAHLRDELGDLLFQVVFHARMAEERGLFDFGGVVE
                     AITGKMIARHPHVFGGKETPESAAAQTVAWEEHKRGERAAKPGGLLDDVPRALPALLR
                     AEKLQKRAGSVGFDWESAPKVVEKIAEEAAEIAEAQAAGASAHKLEEEVGDLLFAVAN
                     LARHLKVDAETALRAANAKFVRRFRYIEAGLATRGKTPAEASLEEMEMLWVEAKKSE"
     gene            2410..2913
                     /locus_tag="VGC27_00490"
     CDS             2410..2913
                     /locus_tag="VGC27_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0281087.1"
                     /translation="MRFAAALLFLGLCALPCRAAGPADFAGRILAVHNAERAALKLPP
                     LVWNETLAAHAAVWARHMAETGKPRHSPKDQRKGEGENLWVGTAGSFSPEGMVGGWVK
                     EKQFFRNGTFPDVSTPGEGHVVGHYTQMIWKNTSKVGCAKVSGGGWDMLVCRYSPEGN
                     LIGEKPY"
BASE COUNT          630 a         1040 c         1056 g          582 t
ORIGIN      
        1 ttcatgtcga gcccaaatcg cgcaaaccgt cggacgacct tgatcgcgac gcgagcgccc
       61 ggctggccga agccgtaggc ttgaccaagg cgatagggct tgaagtggtc gccgaattga
      121 tcgtcccgct ggcgcgggcc gttccggcga ccctgatcgg cagcggcaag gttgacgaca
      181 tcgcctcgca agtgcgagcg ctcgagcccg agcttgtcat cgtcaacacc cagctttcgc
      241 cggtccagca gcgcaatttg gaaaaggcct ggagctcgaa ggtgctggac cgcaccgcgc
      301 tgatccttga aatcttcggg gaacgggcgc gaacccgcga aggccggctt caggtggaac
      361 tggcgcatct tacttggcag cgcagccgcc tggtccggtc gtggacccat ctggagcgcc
      421 agcgcggcgg cttcggcttt ctcggcggac cgggcgaaac ccagatcgaa accgaccggc
      481 gcctgatcgg cgaacgcatc acaaagatca agaaggagct tgaaggcgtc aagcgcaccc
      541 gcggcttgca gcgcaaggcg cgcaagcggg tgccgttccc ggtgatcgct ctggtcggct
      601 acaccaacgc cggcaagtcc acgctgttca acgccctgac caaggccggc gtgctggcca
      661 aggacattct cttcgccacc ctcgatccga ccatgcgcgg gattcgcctg ccgggcggaa
      721 ccaaggcggt cctgttcgac acggtggggt tcatcgccga cctgccgcac gagctggtcg
      781 ccgccttccg cgccacgctg gaggaggtgc tggaggccga catcattgcc catgtgcgcg
      841 acgccgctca tgccgaaacc gaagcccaga aggccgatgt actggccgtc ctggccgaat
      901 tgggcgtgga gccgagagtg tttccaggcg aagtgtgcag cggttcgccg tcgaaaacgc
      961 gacaaaatat agatttccgc gaccggccgc tcgtggaaat cctcaataag atcgacctgt
     1021 tggagcccgg cgctcgcgca ggattgaagg ccagcgcagc ggagcgcccc gaagtccccg
     1081 ccgccgtatc ggcgctgacg ggcgagggac ttgatgatct cctaaggcgt ttcgagtcgg
     1141 tgttgacggg tggcaacatc tgcctccgtc ttgagctttc gccatccgac ggtgagggac
     1201 tggcctgggt ctatcgccat ggccaagtac tcgaacgcga cgacggcgag gccggaattg
     1261 tcctgaccct cagcgtccat ccacaggacg cggcgcggtt tgaaagccgc tttgcaggga
     1321 aaatatcggt ggaacaggat attgccgccg acatctgatg tgaattgccg ggtaggggat
     1381 atgtggaaaa ggcgagtagg gagtagcgaa tagcgagtag aaaaagtttc cctattcgct
     1441 actcgccatt cgctactcgc tctttttcgc ctccacccac agcatttcca tctcctccag
     1501 gctcgcctcg gccggcgtct tgccgcgcgt cgcaagtcca gcctcgatgt acctgaagcg
     1561 gcgaacgaat tttgcgttcg cggcgcgcag cgccgtctcg gcatcgacct tgaggtggcg
     1621 cgccagattg gcgaccgcga acaacaggtc gccgacttcc tcttccagct tgtgcgcgga
     1681 agcaccggct gcctgcgcct cggcgatctc ggctgcttcc tcggcgatct tctcgaccac
     1741 cttaggggcg ctttcccaat cgaatccgac gcttccggcg cgcttttgaa gtttttccgc
     1801 ccgcaacagc gcgggcagag cgcgcggcac atcgtccagg agtccacccg gcttggcggc
     1861 tctttcgccg cgtttgtgct cttcccaggc caccgtctgg gcggcggcgg attccggcgt
     1921 ttccttgccg ccgaacacat gggggtggcg ggcgatcatt ttgccggtga tcgcctcgac
     1981 cacgccgccg aaatcgaaca ggccacgttc ttcggccatg cgggcgtgga agacgacctg
     2041 gaacagcagg tcgcccagct cgtccctcag atgcgcccag tcgcggtctt cgatggcgcc
     2101 ggctacttca taggcttcct cgatcgtgta aggcgcgatg gtcgcgaaat cctgctcctt
     2161 gtcccaaggg cagccgcgca cggggtcgcg cagcgaccgc atgatctcaa gaatcgattc
     2221 gatgtctctg gagggattcg gtttttccat gccgcgatag aaggcgggca ggcgcctcac
     2281 ggcaaggggc gcccgccttt tttacacagc gcggcggggc ggctgccgct gcggcaccgg
     2341 tgaaccgagg ccgggaggcg tgtatatttc ccgccggaaa cggatgcagc cccatcttgc
     2401 gaggcgaaca tgagatttgc ggcggcgctt ttgttcctgg gcctgtgcgc gcttccttgc
     2461 cgggcggcgg gcccagccga tttcgccggg cggattctgg ccgtgcacaa cgccgaacgg
     2521 gccgcgctca agctgccgcc gctggtctgg aacgaaacgc ttgccgcgca tgccgccgtc
     2581 tgggcgaggc atatggctga aacaggcaag cccaggcata gccctaagga ccagcgtaag
     2641 ggcgagggag agaatctctg ggtgggtacg gccggaagct tctcgccgga aggaatggtc
     2701 ggcggctggg tgaaagagaa gcagtttttc cgcaatggga cctttccgga cgtcagcacg
     2761 ccgggcgaag gtcatgtggt gggtcactac acgcagatga tctggaaaaa cacgtccaag
     2821 gtcggctgcg ccaaggtgag cggaggcggc tgggacatgc tcgtctgccg ctacagcccg
     2881 gaagggaacc tgatcgggga gaagccttac tagacggcac gggacggcgt cggtcggcac
     2941 aaaacaactt cgcccatcgc gcataaattc gtataagata tattatggga aatactaggt
     3001 ggttgtgcgt ctggctcaac gggcgcagaa tttattccag ccattaccgg cacggagcca
     3061 tccatgggta cgagtcccaa gaaaccaaag aaaccagtgt ccaaaaagac gacgactgcc
     3121 gatcagcagc ccgaccagcc gaaagagccg ccagcagccc aagccgccgc gaaacacccg
     3181 ccgccgccca ggtcgctccc ggccggcatc ccgcccaaca tctcgaacat gttcgggagc
     3241 aaaggcggca agggtcccag gttcacagcc accaagggcc gcaacttccg ccaccagggc
     3301 cgttaacg
//
LOCUS       DASWPU010000024         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_18558,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000024 DASWPU010000000
VERSION     DASWPU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18558"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VGC27_00495"
     CDS             <1..468
                     /locus_tag="VGC27_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HEY0281088.1"
                     /translation="KIEFEQLRGFEGIRTATIDMGNSIGVVKVAMAHGLKPTRELCEL
                     VKNGEADFDFIEIMACPGGCVDGGGNLRSKKAYMPNALKRRATLFSIDRERRVRQSHN
                     NEQIKALYRDFLEKPYSDKAHHLLHTMYNDRKLTLTRSVREIWGEITMSTMVY"
     gene            995..1495
                     /locus_tag="VGC27_00500"
     CDS             995..1495
                     /locus_tag="VGC27_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY0281089.1"
                     /translation="MDAQSQLQSEVGRLVREYGRNRTALMPILQDLNRTHSYISEDAM
                     LCVAKELDVPRTEVYGVATFYSFFSVKPRGKYVIRTCRTISCAKQGHYHSVLVALLQE
                     LGIKFGETTADGLFTLEQTNCNGMCDQGPAMLVNDDVHVKLTETSVREILNLYRHKAS
                     EQGTTV"
     gene            1515..2711
                     /locus_tag="VGC27_00505"
     CDS             1515..2711
                     /locus_tag="VGC27_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY0281090.1"
                     /translation="MDVKTVRGFVGNKLSFADVSAEEGLKMAASMAAEDIIAIIDASG
                     LRGRGGAGFPSGKKWELARGQEGSPKYIIGNADEGEPGTFKDRLIMIDYPDLLFAGMT
                     IAAIAIGAEKGMLYLRAEYAWMREHLEGVLARRRSAKLLGPDALGRKGFNFDITIRMG
                     SGAYICGEETALIESLEGRRGDARNRVPFPVQAGYLGKPTVVNNVETLAWVPCIIQKG
                     PSWFASMGTRKAPGAWLFSVSGDCKRPGVYEYPFGTSLRQILVDAGAEDAKGVQVGGA
                     SGICVPPEQFDRTLTPGDLATGGSIIVFGPKQDMFAVAENFQHFFADESCGQCIPCRV
                     GNVRLLEAIHRMRKEGLDEDTEHTLRQLAKTMQVASKCGLGQSSPKAFLSILDKFAGE
                     LPARSA"
BASE COUNT          600 a          802 c          867 g          513 t
ORIGIN      
        1 aagatcgaat tcgagcagct acgcggcttc gagggcatcc gcaccgccac tatcgatatg
       61 ggcaattcca tcggcgtggt gaaggtggcc atggcccacg gcctcaagcc gacgcgcgaa
      121 ctgtgcgaat tggtgaagaa cggcgaggcc gatttcgact ttatcgagat catggcgtgc
      181 cccggcggct gcgtggacgg cggcggcaat ctgcgttcca agaaggccta tatgccgaac
      241 gccttgaagc gccgcgccac gctgttcagc atcgatcgcg agcgcagggt gcgccagtcg
      301 cacaataacg agcagatcaa ggcgctatac cgcgacttcc tcgagaagcc ttattcggac
      361 aaggctcatc atctgcttca caccatgtac aacgaccgca agctgacgct gacgcggagc
      421 gttcgggaaa tctgggggga aatcaccatg agcacgatgg tgtattgaag cttccgcccg
      481 gtgcgacaat gagtctttga cttcagcctg tcctaaggca ttgattgaac agcaagattt
      541 gtaatgcagc gcgcggttgc agcgccgttt gtaatattcc aacaaagctt tgatttttca
      601 caatgatttc ggagggttgc aggccctagc tgggctgcaa tttgaaatgt tctaactata
      661 gagatgggca tgatctgtct cggcatttcg cgcgagaatg tgcggctcga tctcttccgg
      721 gtccacgagg ctcttgcaac gcaccgtccg ggcggccgcg cgcgggcgcg atcttcttgg
      781 attgcctatt cggccacgcc tcgcctgagg cgcagcagtt cggggagtaa ggtcgcacgc
      841 gtagaaatgt cgcatatggg cgacaaggaa gcggatatct ccgaacgcat catgagggcc
      901 ataaggtaag gaaaaaagga aacgtttact agcagggggc gcaccgggaa tacgggcgca
      961 cagataattt agggcggaaa ccgaggagca ggagatggac gcgcaatcgc aacttcagtc
     1021 cgaagtaggg cggcttgtcc gcgaatatgg ccgcaaccgc acggcgctga tgccgatcct
     1081 tcaggacttg aaccgcaccc acagctacat ctcggaagac gcgatgctgt gcgtggcgaa
     1141 ggaattggat gtgccgcgga cggaggtcta cggggtcgcg accttctaca gttttttcag
     1201 cgtcaagccg cgcggcaaat acgtcattcg cacctgccgt acgatcagtt gcgccaagca
     1261 gggccattac cattccgttc tggtggcgct gctgcaggaa ctgggaatca aattcggcga
     1321 gaccacggcg gacgggcttt tcacgctcga acagacgaac tgcaacggca tgtgcgacca
     1381 gggaccggcc atgctggtga acgacgacgt gcatgtgaag ctgaccgaaa cctcggttcg
     1441 ggagattctg aacttgtatc gccacaaggc gtcggaacag ggaacgaccg tctgatatag
     1501 gctgcgagga caaaatggac gtgaagactg tacgcggatt cgtgggcaac aagctttcct
     1561 tcgcggacgt ctccgcggag gaagggttga agatggccgc cagcatggcg gccgaagaca
     1621 tcatcgcgat catcgacgcc tcggggctca gaggccgcgg cggcgcgggc tttcccagcg
     1681 gcaagaaatg ggaactcgcc cgcggccagg aaggctcacc caaatatatc atcggcaatg
     1741 ccgacgaggg cgagcccggc accttcaaag accggctgat catgatagat tatccggatc
     1801 ttctcttcgc cggcatgacc atcgcggcca tcgccatcgg tgccgagaag ggcatgctct
     1861 atctccgcgc cgaatacgcc tggatgcgcg agcatctcga aggcgtgctg gcgcgccgcc
     1921 gcagcgccaa gctgctcggc ccggatgctc tcggccgcaa gggtttcaat ttcgacatca
     1981 ccatccgcat gggctccggc gcctatatct gcggcgagga aaccgcgctg atcgaatcgc
     2041 tggaagggcg gcgcggcgac gcgcgcaacc gggtcccgtt cccggtccag gccggctatc
     2101 tcggcaagcc cacggtggtg aataatgtgg agacgctggc ctgggtgccc tgcatcatcc
     2161 agaaagggcc gtcctggttc gcctcgatgg gaacgcgcaa agcccccggc gcttggctgt
     2221 tttccgtctc gggcgactgc aagcgtcccg gcgtttacga atatccgttc ggcaccagcc
     2281 tgcgccagat tctggtcgat gcgggcgcgg aagacgccaa gggcgtgcag gtcggcggcg
     2341 ccagcggcat ttgcgtgccg cccgaacaat tcgaccgcac gctgactccg ggcgacctgg
     2401 ccaccggcgg ctcgatcatc gtgtttgggc ccaagcagga catgttcgcg gtggcggaga
     2461 atttccagca tttcttcgcc gacgaatcct gcggccaatg cattccctgc cgggtcggca
     2521 atgtcaggct gctcgaagcc atccatcgca tgcgcaagga aggcctcgac gaagacaccg
     2581 aacacacgct ccgtcagttg gccaagacga tgcaggtggc ctcgaaatgc ggactgggcc
     2641 aatcgagccc gaaggcattt ctgtccattc tcgacaaatt cgcgggcgaa ttgcccgcgc
     2701 gcagcgcgta ggcgaggaag aggaacctct catgacggca gatacgatta ccatctcgat
     2761 cgatggaaaa gaactgaaag tc
//
LOCUS       DASWPU010000025         5017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_110248,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000025 DASWPU010000000
VERSION     DASWPU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110248"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..2168
                     /locus_tag="VGC27_00510"
     CDS             423..2168
                     /locus_tag="VGC27_00510"
                     /inference="COORDINATES: protein motif:HMM:NF036639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaF N-terminal domain-containing protein"
                     /protein_id="HEY0281091.1"
                     /translation="MSGTPYPKAQMHRIAYTKLSAREIESRLSKAASGGPASDSPLSD
                     VFAGKSIRIATDKGLGLSYGFRGKNRLTVSENGGAALEAGYGALALDHIVVFTHLVPG
                     TQRGYAVVVDQNTNLATVFELWFSGYEDNREVQREIYYGYVEQPGRNPSEARHAATNR
                     MEGHGYHWRQDSGIETLEFFPSAAYSHFVELTRLGGELGFCAPSDYIKIDNEYFIYTR
                     TECEFSGIFTLYVIGLNLVEQVGLRLGFNAGDALEYYVFRGKGEWLGQLAHFEKFGDF
                     KGDVVPVPVEDDKPPAKGARPVYRPMKTWTVMSKAEVDAVARKATVYDPESAMAGNAD
                     PPTDFLAGKKLTLRYDNAPAMEYRFDGPETLHWRKEGGRSWVKARYKAWESMPGVIVF
                     GHLLEGEPDHDGHSIVADFHQGLVTCFNGYLNTPYFANEAGVKTLFGVIEMEGIIPPL
                     YRRHSFSDEMAGRALTWEYSPGLTSMHFYSTPHTASWIIFTESGAGGMEWSGPASYVK
                     IRDGLYFAYWLEEACNGTLGTILINLHTMHDAGVSYKCDKEGVHMHPVGALGRHAGKF
                     DVARFYQVKSSGRTA"
     gene            2165..2662
                     /locus_tag="VGC27_00515"
     CDS             2165..2662
                     /locus_tag="VGC27_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281092.1"
                     /translation="MSRNDISTRRTFLKAGAVLSAPLAAAPVAAFAGDSEARLARLED
                     ENAIRVLHQTWLRSINAGTAGKERVPPLLAGVQAAGDKVRSIAANHAAPPDVIMLAAD
                     GRNATGRFHCTIGLERPIAQDCTLAQMAHLQGGGFVRRTESRVLIVDYVKAGGAWSIA
                     RVESA"
     gene            complement(2747..>5017)
                     /locus_tag="VGC27_00520"
     CDS             complement(2747..>5017)
                     /locus_tag="VGC27_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281093.1"
                     /translation="LEGRIVTYRYAATAAKAKIWDLDNPNLYRLNAKWTAGRGASTRM
                     VRFGFRWFDIVGIGSDAHFTLNGRRIKLYSAISWGYWAFNGLWPTPDLAEKEVTQAKA
                     LGLNCLAFHRNPGKHDVLAAQDRLGLLRGMEPGGGKFALGRMPDGTNVGQHSIVMQPP
                     ESEADKFAQGYLLVNTIAMMRAFRSHPSLVHWTVQNDNNADFKDPTATAVLAALRADD
                     ESRIILLNDGMSSPQFNAAQAFYAPYDAKLHRSDQEPWGGWWGDHQGAGDQWYDEFYK
                     DPQHFTYQNPIREQIVQYGEMEGCAVPDNHTLLVAEILRRGGKSYDLNDHKDMLAAYQ
                     SFLDRWNFRGAFPNAESLFLAIGRKSYASWQEYMENARINDETDYAVISGWESTAIDN
                     HSGIVDNQRNFKSDPALIQGSLLPVRPVAKQRSLVAAKGDSVLFDFYLLNDTGKPVTG
                     QLSFSMTDPAGQKSVLAEMAAPAMQPGVFSALIQEGFATPPLTQEGFYTFEYALPGVP
                     AQTRQIWVTDAKRAPVGGKVLRVGVAGVWPSLRAQLAKLPGITVEEFSSSRFDVLGLG
                     RGRYDVLISSGLTEQSSPGQKLGGEEGLAMQRKSGQPAVPGALPDGMLDAIKDGTPLL
                     LLPQEDALSDGVAKQLAAAGAFRYGGDVGRLRAPWMGNWYFLRSHPVFAGMPVNRAMD
                     GYFQARGRLANGLIVDGPEVEVFVGYSRDHERRVGAGTFSARLGKGRILFQRVPDVLA
                     PLQLRFLSNALGWLCA"
BASE COUNT          896 a         1537 c         1632 g          952 t
ORIGIN      
        1 ggcgacaagc attttcttga tcatgacatt ctccctgttc ctggccgctt cttctttctc
       61 tgcgtagata cgatcgtcaa cggatcgtcg gcattggcgc gtttcgttcg ggcagtatag
      121 gcgcaattcc cggccattct atgcgtgtgt cgttccgcca aatccgtatg gttgttggcg
      181 caggaaatcg gctgcatggt tccgccaata gccttgtcag gcgccgcatg atgtccgcgt
      241 cggtatagcc gaggttcacg gaaaacgctg tcctgctctt ttgcgccggt gtgccgaata
      301 tgaatacgga gtatgttgcg ggagtgccga atgcacagca aaatcctcgt cagtcaagct
      361 gatgacctca tgtcatttgc ataatccgcg tatagacgga tcaccatagg gaggcagaag
      421 aaatgtcggg gacaccgtat cccaaagcgc agatgcatcg catcgcttat accaagcttt
      481 ctgcaagaga gatcgaaagc aggctgagca aggccgcgtc gggcgggccg gcaagcgact
      541 ctccgctttc ggatgtgttc gccggcaagt cgatccggat tgccacggac aagggcctcg
      601 ggctcagcta cggctttcgc ggcaaaaatc gcctgacggt gtccgagaac ggcggcgcgg
      661 cgctggaggc cggctatggc gcgcttgcgc tcgatcacat cgtcgtcttc acgcatctgg
      721 tccccggcac gcagcgcggc tacgccgtgg tcgttgacca gaacacgaat ctggcgaccg
      781 tcttcgagtt gtggttcagc ggctatgaag acaatcgcga ggttcagcgc gagatctatt
      841 acggctatgt cgaacagccg gggcggaacc cgtccgaggc gcgccatgcc gcgacgaacc
      901 gcatggaagg ccacggctat cattggcggc aggacagcgg catcgagacg ctggagttct
      961 ttccttccgc agcctattcg catttcgtcg aactgacgcg gctgggcggc gaactcgggt
     1021 tctgcgctcc ttccgactac atcaagatcg ataacgagta tttcatctac acgcgcacgg
     1081 agtgcgagtt ctccggcatc ttcacgctct atgtgatcgg cctgaacctt gtcgagcagg
     1141 tcgggctgag gctcggcttc aatgccggcg acgcgctcga atattatgtc ttccgcggca
     1201 agggcgagtg gctcggccag cttgcgcatt tcgagaagtt cggcgacttc aaaggcgatg
     1261 tggtgcctgt gccggtcgaa gacgacaagc cgcccgccaa aggcgcgcgc cccgtctatc
     1321 gcccgatgaa aacctggacg gtcatgtcca aagcggaagt cgatgccgtc gccaggaagg
     1381 cgactgttta cgatcccgaa agcgccatgg ccggcaatgc cgatccgccg acggattttc
     1441 tcgccggcaa gaagctgacg cttcgctacg acaatgcgcc ggcgatggaa taccgcttcg
     1501 acggtcccga gacgctgcac tggcgcaagg aaggcggcag aagctgggtc aaggcgcgct
     1561 acaaggcgtg ggaatcgatg cccggcgtga tcgtcttcgg ccatctgctc gaaggggagc
     1621 cggaccatga cgggcattcg atcgtcgccg atttccatca aggcctcgtc acctgtttca
     1681 acggctatct caacacgccg tatttcgcca acgaggcggg cgtgaagacg ctgttcggcg
     1741 tcatcgagat ggaggggatc attcctcccc tctaccggcg ccatagcttc agcgacgaga
     1801 tggccggccg cgcgctgacc tgggaatatt cgcccggcct cacctcgatg catttctatt
     1861 cgacgccgca tacggcgtca tggatcatct ttaccgagag cggcgccggc ggtatggagt
     1921 ggagcggccc cgcgtcctac gtcaagatcc gcgacggact ctatttcgcc tattggctgg
     1981 aagaggcctg caacggcacg ctcggcacga tcctcatcaa tctgcacacg atgcacgatg
     2041 cgggcgtcag ctataagtgc gacaaggaag gcgttcacat gcaccccgtc ggtgcgctgg
     2101 ggcgacatgc cggaaagttc gacgtggcgc gcttctacca ggtgaagagt tcggggagga
     2161 cggcatgagc aggaacgaca tctcgacccg gcgtactttt ctgaaggccg gcgccgtgct
     2221 ctccgcgcct ttggcggcgg cgcccgtggc ggcgttcgcc ggcgacagcg aggcgcgtct
     2281 ggcgcggctc gaggacgaaa acgccattcg cgttcttcat cagacgtggc tgcgcagcat
     2341 caacgcaggg acggcgggca aggagagggt gccgcccttg cttgccggcg tgcaggcggc
     2401 cggggacaaa gtccgcagca ttgccgcgaa ccacgcagca ccgcccgacg tgatcatgct
     2461 cgcggcggac ggcaggaacg caaccgggcg cttccattgc acgatcgggc tggagaggcc
     2521 gattgcgcag gattgcacac tggcgcaaat ggcgcatctg cagggcggcg gtttcgttcg
     2581 ccggaccgag tcccgcgttc tcatcgtcga ctacgtaaag gccggcggtg cttggtcaat
     2641 cgcgcgggtc gagtcggcgt aacgccgccg gctgcgtcgc ggatcattgc cggggcctgt
     2701 atcggcagcg tggttttgcc gttcgtctcg ttatctcacc ggcgcctcag gcacacagcc
     2761 agcccagtgc gttgctgagg aagcgcaact gcagcggggc cagcacgtcg ggtacgcgct
     2821 ggaacaggat cctgcccttg ccaaggcggg ccgagaaggt tcccgcccca acccggcgtt
     2881 cgtgatcgcg gctgtagccg acgaacacct ccacctccgg gccatcgacg atcaggccgt
     2941 tggccagccg gccgcgcgct tggaaatagc cgtccatggc gcggtttacg ggcatcccgg
     3001 caaagaccgg atggctgcgc aggaaatacc agttgcccat ccagggcgcg cgaagacgtc
     3061 ccacatcacc gccgtagcgg aacgcgcctg ccgccgcgag ctgtttggcg acgccgtcag
     3121 agagcgcgtc ttcctgaggc aggagcaaga gcggcgtgcc gtccttgatc gcgtcgagca
     3181 tgccgtcggg cagggcgccg ggcacggcgg gctggccgga tttcctttgc atcgcgaggc
     3241 cttcctcgcc gcccagcttc tggccgggcg aggactgctc ggtgaggccg gaggagatga
     3301 gcacgtcgta gcggccgcgg ccgaggccca gcacgtcgaa gcggctgctg ctgaactctt
     3361 ccaccgtgat gcccggcagt ttggccagtt gcgcccgcag cgaaggccac accccggcca
     3421 cgcccacgcg caagaccttg ccgccgaccg gcgcacgctt ggcatcggtg acccagattt
     3481 ggcgcgtctg ggcggggacg ccgggcagcg catattcgaa agtatagaaa ccttcctgcg
     3541 tcagcggcgg ggtggcgaag ccttcctgga tgagggcgct gaagaccccc ggctgcatcg
     3601 cgggcgcggc catctctgcc agcacgctct tctgcccggc gggatcggtc atgctgaagc
     3661 tcaactgtcc cgtcaccggt ttgccggtat cgttcaagag atagaaatcg aacagcacgc
     3721 tgtcgccctt cgccgcaacc agcgaacgct gcttggccac cggccgcacc ggcaagaggc
     3781 tgccttggat cagcgccggg tcgctcttga agttgcgctg gttatcgacg atgcccgaat
     3841 ggttgtcgat ggcggtggat tcccagccgc tgatcaccgc gtaatcggtc tcgtcgttga
     3901 tgcgcgcatt ctccatatat tcctgccaac tggcatagct cttgcggccg atggcgagga
     3961 acaggctttc ggcattgggg aaggcgccgc ggaaattcca gcggtcgagg aacgactgat
     4021 aggccgcgag catgtccttg tggtcgttga ggtcgtagct cttgccgccg cgccgcagga
     4081 tttcggccac gagcagcgtg tggttgtcgg gcacggcgca gccttccatc tcgccgtatt
     4141 gcacgatctg ctcgcggatc ggattctggt aggtgaagtg ctgcgggtcc ttgtagaact
     4201 cgtcgtacca ctggtcgccg gcgccctgat ggtcgcccca ccagccgccc cagggctcct
     4261 ggtcggagcg gtgcagtttg gcatcgtaag gcgcatagaa ggcctgggcg gcattgaact
     4321 ggggcgagga catgccgtcg ttcagcagga tgatgcggct ttcgtcatcc gcccgcaggg
     4381 cggcgagaac ggccgtcgcc gtggggtcct tgaaatcggc gttattatcg ttctgcaccg
     4441 tccagtgcac cagactggga tgggagcgga aggcccgcat catggcgatg gtgttgacca
     4501 gcaggtagcc ctgggcgaat ttgtcggctt cgctttccgg aggctgcatc acgatgctgt
     4561 gctgcccgac attggtgccg tcgggcatgc gcccgagggc gaatttgccg ccgcccggtt
     4621 ccatgccgcg caagaggccg agccggtcct gcgccgccag cacgtcgtgc ttgccgggat
     4681 tgcggtggaa ggcaaggcaa ttgagtccca gagccttggc ttgcgtcact tccttttcgg
     4741 ccaggtcggg cgtcggccac aggccgttga aggcccaata gccccaactg atggccgaat
     4801 agagcttgat gcgccgtccg ttgagcgtga aatgcgcgtc gctgccaata ccgacgatgt
     4861 cgaaccagcg gaagccgaag cgcaccatgc gcgtggaagc gccgcgcccc gccgtccatt
     4921 tggcgttcaa acgatagaga ttgggattgt cgaggtccca tattttcgcc ttcgcggccg
     4981 tcgcggcata acgataggtg acaatgcgcc cttcgag
//
LOCUS       DASWPU010000026         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_91928,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000026 DASWPU010000000
VERSION     DASWPU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91928"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..787
                     /locus_tag="VGC27_00525"
     CDS             2..787
                     /locus_tag="VGC27_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HEY0281094.1"
                     /translation="MRSASPLSDDQIRTVAPSIFAINKHDSRSERYTYIPTIDVLNGL
                     RHEGFQPFMVCQTRVRNEGKREHTKHMIRLRHAADIGSAEANEIVLLNSHDGSSSYQM
                     LAGMFRFVCQNGMVCGDTVGDIRVPHKGDVIGEVIEGAFKVLDSFEAATHQREEMAGL
                     ALTDGEQTAFARAALTLRYDDDKPAPITEQQLLTPHRMEDRAPDLWTTFNRVQENMIS
                     GGLRGRNANGRATTTRAVTGIDQNIRLNRALWVLADELCRLRD"
     gene            826..1137
                     /locus_tag="VGC27_00530"
     CDS             826..1137
                     /locus_tag="VGC27_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281095.1"
                     /translation="METDMDTAHIRPTWLTALLPLLAVYQYGDRSLVRAELFRMALSA
                     DAAARSAEALGRIANMLDRDGNPIDMHREELRAIARNALSEFDRVPPRAPQSALPEPR
                     F"
     gene            complement(1134..>1580)
                     /locus_tag="VGC27_00535"
     CDS             complement(1134..>1580)
                     /locus_tag="VGC27_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281096.1"
                     /translation="GNGSGVNRGRRGLFLTPRALRPLEFARCAEFEIIDREVVAVALD
                     AFTVCEIVVRQLRFNDQLIALQNVSGDGLTARTERRKLDSQLTLFRFAAAFVATGVLV
                     GNQCEFRHLRITVIRKHWGGSNTACKKILFMICTPESVNETEGDLR"
BASE COUNT          346 a          500 c          444 g          290 t
ORIGIN      
        1 gctgcgctcg gcctcgccgc tgtcggacga ccagattcgc accgtcgctc cgtcaatctt
       61 cgccattaac aagcacgaca gccgctccga gcggtacacc tatatcccca ctatcgacgt
      121 actcaatggt ctgcgtcacg aaggctttca gcccttcatg gtctgccaga cccgcgtgcg
      181 caatgaaggc aagcgcgaac acaccaaaca catgattcgt ttgaggcacg ccgcagacat
      241 tggtagcgcg gaagccaatg aaatcgtcct gctcaattca catgacgggt cgagcagcta
      301 tcagatgctg gctggtatgt tccgcttcgt ttgccagaac ggcatggtct gcggcgacac
      361 cgtcggcgat atccgtgtgc ctcacaaggg tgacgtgatc ggcgaggtga tcgagggagc
      421 gttcaaggtg ctcgacagct ttgaggcagc gacccatcag cgcgaagaga tggccggtct
      481 ggcattgacc gacggcgaac agaccgcttt cgcacgggcc gcgcttacgc tgcggtacga
      541 cgacgacaag cccgcaccca tcactgagca gcaacttcta acaccgcatc gcatggagga
      601 tcgcgcacca gacctgtgga cgacattcaa tcgcgtgcag gaaaacatga tcagcggcgg
      661 cctgcgcggt cgtaatgcga acggccgggc aaccacaacc cgagccgtga ccggcattga
      721 ccagaacatc aggctcaacc gcgccctctg ggtgctcgcc gacgaactgt gccgcctgcg
      781 cgactgacag gtgcgcccgg ccctcgggcc gggcatgacc tcactatgga aaccgacatg
      841 gataccgctc acatccgccc aacgtggcta accgccctcc ttccgctgct agccgtctat
      901 caatacggcg atcgttctct ggtgcgcgcg gaactgttcc gcatggccct gtccgccgat
      961 gccgcagcgc gctccgccga agcactcggc cggattgcca acatgctgga tcgcgacggg
     1021 aacccaatcg acatgcaccg ggaagagttg cgcgcgatag cccgcaatgc tctctctgag
     1081 tttgatcgcg tgccgccgcg tgccccgcag tctgccttac ccgagccaag attctaacga
     1141 aggtctccct ctgtttcatt cacactctca ggagtacaaa tcatgaacaa aattttttta
     1201 caggccgtgt tgctgccgcc ccaatgcttt cgaatcacgg tgattcgaag gtgacgaaat
     1261 tcacactgat ttccaacgag tacgccggtc gcgacgaaag cagcggcgaa gcgaaagagc
     1321 gtcagctgag aatccagttt acggcgttcg gttcgcgcgg tgaggccatc gccagaaacg
     1381 ttctgaaggg cgatcaactg atcgttgaag cgaagctgtc gaacaacgat ttcacagacg
     1441 gtgaaggcat cgagcgctac agctacaact tcacggtcga taatttcgaa ttcggcgcac
     1501 cgggcgaact caagcgggcg caacgcgcgc ggcgtcagga ataatccacg tcggccccgg
     1561 ttcacaccgg agccgttgcc
//
LOCUS       DASWPU010000027         2610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_110258,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000027 DASWPU010000000
VERSION     DASWPU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110258"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VGC27_00540"
     CDS             complement(<1..730)
                     /locus_tag="VGC27_00540"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF007989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY0281097.1"
                     /translation="MTALPDRMAMEIKQDALAAIGNTPLIRLRRASEETGCEILGKAE
                     FMNPGQSVKDRAALFIVEDAIATGRLRPGGVIVEGTAGNTGIGLAVVGNAMGFRTVIV
                     IPDTQSVEKKDALRLLGAQLIEIPAVPHRDANNYVKISGRIAEQLAQSEAAGAVWANQ
                     FDNVANRRAHEKGTGPEIWRQTNGRVDGFVSAVGTGGTLAGVAMALKERRPSVHIAAA
                     DPLGAAIYSWIKTGELKAHGTSITE"
     gene            993..1235
                     /locus_tag="VGC27_00545"
     CDS             993..1235
                     /locus_tag="VGC27_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281098.1"
                     /translation="MGTVLRAVFAFAVAYAVYAPHEFAAHFETPRFAAVKADLAAAQP
                     GVKLQRMIEDGLKRLPDRIAAEANGSPGHVGGHRLL"
     gene            complement(1264..1338)
                     /locus_tag="VGC27_00550"
     tRNA            complement(1264..1338)
                     /locus_tag="VGC27_00550"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1304..1306),aa:Asn,seq:gtt)
     gene            1423..1692
                     /locus_tag="VGC27_00555"
     CDS             1423..1692
                     /locus_tag="VGC27_00555"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEY0281099.1"
                     /translation="MTVTQHHQSTIRFGRFRVKVPGTRLQRTILGIGLCIGGVLGFLP
                     IVGFWMLPLGILVLSLEYHPIRRLRRRFEVRWGRWRRKDSVAKKR"
     gene            complement(1842..1915)
                     /locus_tag="VGC27_00560"
     tRNA            complement(1842..1915)
                     /locus_tag="VGC27_00560"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1881..1883),aa:Cys,seq:gca)
     gene            2247..>2610
                     /locus_tag="VGC27_00565"
     CDS             2247..>2610
                     /locus_tag="VGC27_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0281100.1"
                     /translation="MRIARGFVVALISCMATVPALGADKPAAAPEKPSGFSLLEALSV
                     AYETNPQLGAAQAGLRASDESVAQANAKWRPNINATGTYGFEKYNITGTPGWFGTHPL
                     NAQLGISQAIFRGGRTYAE"
BASE COUNT          514 a          770 c          799 g          527 t
ORIGIN      
        1 cctcggtgat cgaggtgccg tgcgctttca attcgccggt cttgatccag gaatagatcg
       61 cggcgcccaa aggatcggcg gccgcgatgt ggacggaagg gcggcgttcc ttgagcgcca
      121 tcgccacgcc ggccagcgtg ccgccggtgc cgacggcaga gacgaaaccg tcgacgcgtc
      181 cgttggtctg ccgccaaatt tccggtccgg tgcctttttc atgcgcccgg cggttggcca
      241 cattgtcgaa ctgattggcc cagaccgcgc cggccgcttc gctttgcgcg agttgttcgg
      301 cgatgcggcc ggagattttc acgtaattgt tcgcgtccct gtgagggacg gcagggattt
      361 caatgagctg ggcgccgagc aggcgcagcg catccttctt ttccacgctc tgggtatcgg
      421 gaatgacgat cacggtacgg aagcccatgg cgttgccgac cacggcgaga ccgatgccgg
      481 tgttcccggc ggtgccctcg acgatcacgc cgcccggccg cagccgcccg gtcgcgatcg
      541 cgtcctcgac gatgaaaaga gcggccctgt ctttgaccga ctggccggga ttcatgaatt
      601 cggccttgcc caggatttcg cagccggtct cttccgaggc gcgccggagg cgaatcagtg
      661 gcgtgttgcc gatggcggcg agagcgtcct gcttaatttc catggccatg cggtccggca
      721 gcgccgtcaa cctagtgtcg tggtttcgaa gttcgcacca tccgccatat cggactgcaa
      781 gcgaacttcg aaaccaaaac gacactagga ttcaataggt tgctagcgtc ctttcgattc
      841 cgaaattcgc ccgaagccga tattagagag gaagcgaatt tcggaatcgg gacactagtg
      901 gccccgacag aagccttcaa gttttccccc gccgtcctgc ccagaatttg cgtggggtaa
      961 ggcggaaacg cggttttgtc ccggatcgga ccatgggtac tgtcttgcgc gccgtttttg
     1021 cctttgccgt cgcctacgcg gtctatgcgc cgcacgagtt tgcggcccat ttcgagacgc
     1081 ctcgcttcgc ggccgtgaag gccgatttgg cagccgccca gccgggtgtc aaacttcagc
     1141 gcatgatcga agatggattg aagcgcctgc cggacaggat agccgccgaa gcaaacgggt
     1201 cccccgggca tgtcggggga catcggcttc tgtaaattcg cttcgctcac aagaaaagca
     1261 aactggctcc ccgggccgga ttcgaaccag cgaccaaccg gttaacagcc ggttgctcta
     1321 ccactgagct accggggaac ttgagggcgc gttctctagc gcgtcccgca aggcatggct
     1381 atatcaaact ggaccgccga aaccaattaa tcgacaaggc cgatgaccgt gacacagcac
     1441 caccagtcga cgatccgttt cgggcgcttc cgcgtgaaag tgccgggcac aaggctacag
     1501 cggactattc tgggcatcgg cctgtgcatc gggggcgtac tggggtttct gccgatcgtc
     1561 ggcttctgga tgctgccgtt gggcatcctc gtcctgtccc tcgaatatca tccgattcga
     1621 cgcttgcgca ggcgtttcga ggtgcggtgg gggcgctggc ggcgcaaaga ttctgtcgca
     1681 aaaaagcgct agtacggcac aaaagaaaaa gaaaagggcc gggtgttaac ccggccccaa
     1741 ggcgttgggt aatggtgggg tgtcttcaag ggaagacatc gtcaagatgc cgtatcaagg
     1801 ttaataaagc cttaacatgc ataaacatta agcttgtgag atggaggcca cgcccggaat
     1861 tgaaccgggg tacacggttt tgcagaccgt tgcgtcacca ctccgccacg tggcccggga
     1921 gcgcaatcgg caaaggtgaa gtgcggtttg ccggaaaatt gcgcgaccat caagagcgcc
     1981 cggctatatc agatcgatgc gcccagcgac aacctgccct gcacagactt taacgctagg
     2041 tcaatttgtc accacaccaa cgccaccata cgcctcgcgt tgccggattt tgtgccgctg
     2101 accaggcaaa atccaggatt cggtaggccg caggctcgca aaggcgattt tgttcgcaat
     2161 caatcccggc actagttgga tttcgtaagt cagttggatt cgccttctga catccccaca
     2221 taggaccgtc tttggagggg tatgccatgc gaattgcgcg cggtttcgtc gtcgccttga
     2281 tctcttgtat ggcgacggta ccggctttgg gtgcggataa accggctgcc gcgccggaga
     2341 agccgtcagg gttctcgctg ttggaagcgc tgagtgtcgc ttacgagacc aatccgcaac
     2401 tcggcgcggc ccaggcgggt ctgagggcca gcgacgaaag cgtcgcgcag gccaacgcaa
     2461 aatggcggcc caacatcaac gccaccggca cttacggctt cgagaaatat aatatcaccg
     2521 gtactccggg gtggtttggc acccatccgc tcaatgccca gttgggcatt tcccaggcga
     2581 tcttccgcgg cggccggacg tatgcggagg
//
LOCUS       DASWPU010000028         5010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_91936,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000028 DASWPU010000000
VERSION     DASWPU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91936"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VGC27_00570"
     CDS             <1..434
                     /locus_tag="VGC27_00570"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY0281101.1"
                     /translation="PTSSGVPCEMIELLVNKKSGRRVLGVLIVYNPTENQNLMQVAMP
                     LGVMVQNGAVLSSDTYTSPVLRYRLCDMQGCYAVAPLDDAAVKALGRATKAFMKIVSA
                     DGKKFDLSFSLNGFTAAHSALVDMTRRSASSAPAAAAEPAR"
     gene            831..2321
                     /locus_tag="VGC27_00575"
     CDS             831..2321
                     /locus_tag="VGC27_00575"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HEY0281102.1"
                     /translation="MTNQSDETSRFQAAAEEARRRFDDANGRIKPYLEKASGAFGGFR
                     RWCSKHLPGGERTMWVALGLIVLILFVWAILPGKGTVGRRGFGADGPQPVGVAKAARG
                     DIEVALNALGTVTPLATVTVRPQVSGQLVRVAFEEGQLVKAGDLLAEIDPRSFQAALD
                     QAKGQLARDSALLANARLDLKRNQALFAANAISQQALATQQTLVKQYEGVVLSDRANV
                     DSAAINLGYTRVVAPVSGLAGLRQVDVGNFVGAGQTAGIVVITQLEPISVLFTIPEDN
                     IGAVVSRMKSGAALPAIAYDRGQAAKLATGTLSAVDSQVDTTTGTVKLRALFDNKDGA
                     LFPNQFVNIRLLVNTLHGQIAVPASAIQRGSAGAYVFVVKPDHMVAMRAVTLGPQQDD
                     RVSIVKGLNAGETVVTDGGDRLREGAEVAIPSGQKVDASVAPPKEASAPVQSVQSDRR
                     ALLRKLTPEEREKLRGMSQDERQTWMKDHRDELLKRKDQPGGGRRR"
     gene            2375..>5010
                     /locus_tag="VGC27_00580"
     CDS             2375..>5010
                     /locus_tag="VGC27_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0281103.1"
                     /translation="MNISAPFIQRPVATSLLMIAIMLLGLAGYKYLPLSALPEVDYPT
                     IQVQTFLPGASPDVMTTSVTAPLERQLGQMPGLKQMSSVSSGGASIITLQFDLSLSLD
                     VAEQEVQAAINGASNLLPQSLPAPPIYAKVNPADAPILTLAIHSKTLPLTQVEDIADT
                     RIAQKISQLSGVGLVSINGGQRPAVRVRANIQALAAYGLNIDDLRTTISNANSNAPKG
                     SFDGPSRSYTINSNDQIKTADEYKNIIIAYRNGAPVRLSDVASVEQSAENVNLSSWMN
                     TTPAILLNVQRQPGANVISVADSVKDLLPDIRASLPGSISVDVLTDRTNTIRASVADV
                     QFELELAVVLVVLVIFLFLRNPSATFIPSLSVPLSIVGTFAAMYLLGYSLNNLSLMAL
                     TIAAGFVVDDAIVMIENITRYLEAGESPFDAAMKGAGEIGFTIVSLTVSLIAVLIPLL
                     FMQEVVGRLFREFAVTLAITILISAVVSLTLVPMLCAKLLRRRSERAHPGAVARKAEE
                     YYDVMVREYDRLLRWVLDHQPLTLAVAVGTLVFTVLLYVLIPKGFFPVQDTGFIQGYT
                     RAGPTVSFAEMSKRQEALASVVQQDPDVESLSSFIGVDGTNTTLNGGRMLINLKPKGQ
                     RGDVAATMRRILDLSHQVPGITLYLQPVQDLTIDSTISRAGYQFVLEDASTAVLNQWV
                     PRLMDRLSQAPALRNVSTNYLDRGLSAYVTVDRDTAARFGVTAATIDNALYDSFGQRI
                     ISTIFTESNQYRVILEASPDVQNSVASLSNIYFPAAGGGQVPLAALARVEIKPVPIEN
                     NHLGQFPAAMVSFDVAPSYSLGAAVKAVQKAEADTGLPASINTSFQGSALAFQSTLGN
                     ELLLILAALVTVYIVLGVLYES"
BASE COUNT          891 a         1620 c         1545 g          954 t
ORIGIN      
        1 acccgacctc ttcgggcgtg ccctgcgaga tgatcgagct gctggtgaac aagaaatccg
       61 gccggcgcgt tcttggcgtt ctgattgttt acaatccgac ggaaaaccag aatctgatgc
      121 aggtcgcgat gcccttgggc gtgatggtcc agaacggtgc ggtcctcagt tcggacacct
      181 acacctcccc ggttctgcgt tatcgtcttt gcgacatgca aggctgctat gcggttgccc
      241 cgttggacga cgcggccgtc aaggcgctgg ggcgtgcgac caaggcgttc atgaagatcg
      301 tctccgccga cggcaagaag tttgatctca gcttctcgct gaacgggttc accgccgcgc
      361 acagcgcgct cgtggacatg acgcgcagga gtgcctccag cgcgcctgcg gccgcggcgg
      421 aaccggctcg ctaagagctt agggttcccc cccccttgct cgtccggaac ctgcggaccg
      481 caacggccgc cggataattt gttcatggcg cacgttccgc gtttgttcta gagcgcaatc
      541 cggcaaagtg tgaagcggtt tgccgtcaga ttgcgcgaga atcaaggaat ctagagcttg
      601 atccgattca attaaatcgg atcaagctct agtctgcggc gggttatatt ggttgaagac
      661 cggcgtcgcc ggcgggcttg cgccgatgga cttcgcgaca attcggacaa aattcccggg
      721 aactgctctg ttatctcccc gtccgtctaa cgtacccttc tgcgttgtga atgcttaagg
      781 gatagaacac ggccgttccg cacggccgcg cgtcagcatt ggaagatcgc atgacaaacc
      841 agagcgacga gacgagccgc ttccaagccg cagccgagga agcccggcgc cgttttgacg
      901 atgctaacgg tcgcatcaag ccctatttgg aaaaggcctc gggcgccttc ggcggcttca
      961 ggcgctggtg ctcgaaacat ctgccgggcg gcgaacgcac gatgtgggtc gctctcggac
     1021 tcattgtgct gatccttttc gtgtgggcga tccttcccgg caagggcacc gtgggccggc
     1081 gcggcttcgg cgcggacggc ccacagcccg tgggcgtcgc caaggccgcc aggggcgata
     1141 tcgaagtcgc tttgaatgct ctcggcacgg tcacgccgct ggcgaccgtg accgtcaggc
     1201 cgcaggtgag cggacagctt gtacgcgtcg cttttgagga aggccagttg gtgaaggcgg
     1261 gcgatctgct cgccgagatc gatccgcgca gcttccaggc ggcgctcgat caagccaaag
     1321 gccagctcgc gcgcgacagc gccctgcttg ccaatgcacg cctcgatctg aagcgcaatc
     1381 aggctctttt cgccgcgaac gcgatttcgc agcaggcact ggccacgcag caaaccctgg
     1441 tcaagcaata tgaaggcgtg gtgttgtccg accgggccaa cgtcgattcc gcggccatca
     1501 atctcggtta tacgcgcgtc gttgcgccgg tatcggggct tgcgggtctg cgccaggtcg
     1561 atgtcggcaa cttcgtcggc gccggacaga cggcgggcat cgtcgtcatc acccagctcg
     1621 agccgatatc ggtgctcttc accatccccg aggacaatat cggcgcggtc gtttcgcgca
     1681 tgaagagcgg cgccgcgctt cccgcgatcg cctacgaccg cggccaggcc gcaaagctcg
     1741 ccaccggaac gctatccgcg gtggattccc aggtggatac gacgaccggc acggtcaagc
     1801 tgcgcgcgct cttcgacaac aaggacggcg cgctgtttcc caaccagttc gtcaatatcc
     1861 gccttctggt caacacgctg catggccaga tcgcggtccc ggcgtccgcg atccagcgcg
     1921 gttcggccgg cgcttacgtt ttcgtggtca agcccgacca tatggtggcg atgcgcgccg
     1981 tgacgctcgg tcctcagcag gacgaccgtg tttccatcgt caagggactc aatgccggcg
     2041 aaaccgtggt gaccgacggc ggcgacaggc tgcgcgaagg cgcggaagtg gcgatcccga
     2101 gcggccagaa ggtcgatgcc agtgtcgcgc cgccgaaaga ggcgtcggcg ccggtgcaga
     2161 gcgtgcaatc ggaccgccgc gccctgctcc gcaaattgac tccggaggag cgcgaaaaac
     2221 tgcgggggat gagccaggac gagcggcaga catggatgaa agaccatcgc gacgagttgc
     2281 tgaagcgcaa agaccaaccc ggcggcggcc ggcgccgctg atgtctctcg cgatgcttcg
     2341 gatattccgt caaaggcctt gaaggcgaag tgcgatgaat atttccgcgc ctttcatcca
     2401 gcgtccggtc gcgacgtctc tgttgatgat cgcgatcatg ctgctggggc tggcgggata
     2461 taaatatctg ccgctgtcgg cgctgcccga agtcgattac ccgaccatcc aggtgcagac
     2521 tttccttccc ggcgccagcc cggatgtgat gacgacgtcg gtcacggcgc cgctggaacg
     2581 ccagctcggg cagatgcccg gcctgaagca gatgtcgtcc gtcagttcgg gcggcgcctc
     2641 gatcatcacc ttgcagttcg atttgagcct cagcctcgat gtcgccgaac aggaggttca
     2701 agccgcgatc aacggggcga gcaatctctt gccgcaaagc cttccggcgc cgccgatcta
     2761 cgccaaggtc aatccggcgg acgcgcccat cctgacgctg gcaatccact cgaaaacctt
     2821 gcccctcacc caagtggagg acatcgccga cacgcgcatc gcgcagaaaa tctcgcagct
     2881 ttccggcgtc ggcctggtca gcatcaacgg cggacagcgc cccgccgtgc gcgttcgcgc
     2941 caacatccag gcgctggccg cctacggcct caacatcgac gatctccgga cgacgatctc
     3001 caacgccaat tccaacgcgc cgaaaggcag tttcgacggc ccttcgcgct cctacacgat
     3061 caactccaac gaccagatca agacagccga cgagtacaag aacatcatca tcgcctaccg
     3121 gaacggagcg ccggttcgcc tgtccgacgt cgccagcgtg gagcagagcg cggagaatgt
     3181 gaacctttcg agctggatga acacgacgcc ggccattctg ctcaacgtgc agcgccagcc
     3241 cggcgccaac gtcatcagcg tggccgactc cgtcaaggac ctgttgccgg atatcagggc
     3301 gtccctgccg ggttccatct cggtggatgt tttgaccgac cgcaccaaca cgatccgcgc
     3361 ttcggtggcc gacgtgcagt tcgaactcga actcgccgtg gtgctggtgg ttctggtgat
     3421 cttcctgttc ctgcgcaacc cgtcggcgac tttcatcccc agcctgtcgg tgccgctttc
     3481 gatcgtcggc accttcgcgg cgatgtacct gctcggctat tcgctcaaca atctgtcgct
     3541 gatggcgctg accatcgcgg cggggttcgt cgtcgacgac gccatcgtca tgatcgagaa
     3601 catcacccgc tatctcgaag cgggcgagag cccgttcgac gccgccatga agggtgccgg
     3661 cgagatcggc tttaccatcg tgtcgctgac cgtgtcgctg atcgccgttc tgattccgct
     3721 gctgttcatg caggaggtgg tgggacggct gttccgcgaa ttcgccgtga cgctggcgat
     3781 cacgattctg atctcggcgg tggtgtcgct gaccttggtg ccgatgctgt gcgcgaagct
     3841 tctgcgccgc cgctccgagc gcgcgcatcc cggtgccgtg gcgcgcaagg ccgaggaata
     3901 ttacgacgtc atggttcgcg agtatgaccg tctgctgcgt tgggttctgg atcatcagcc
     3961 gctgacgctc gcggtagccg tcggaacgct ggtcttcacg gttctgctct acgtgctgat
     4021 tcccaagggc ttctttccgg tgcaggacac cggcttcatc cagggctaca cccgggccgg
     4081 gccgaccgtt tcctttgccg aaatgtcgaa gcgccaggaa gcgctcgcca gcgtggtcca
     4141 gcaggacccc gacgtcgaga gcctctcctc cttcatcggc gtggacggca ccaatacgac
     4201 gctcaacggc gggcgcatgc tgatcaacct gaagcccaag gggcagcgcg gcgacgtcgc
     4261 cgcgaccatg cgccggattc tcgacctgtc gcaccaggtt cccggcatca cgctctatct
     4321 gcaaccggtg caggatttga cgatcgactc gacgatcagc cgcgccggct atcaattcgt
     4381 gctcgaagac gccagcaccg ccgtcctcaa tcagtgggtc ccgcgtctca tggatcgctt
     4441 gtcccaggct ccggcgctgc gcaacgtctc cacgaattat ctggatcgcg gtctttcggc
     4501 ctatgtgacg gtggaccgcg acaccgcggc ccgtttcggc gtcaccgccg cgacgatcga
     4561 caacgcgctg tacgactcct tcggccagcg catcatctcg acgatcttca cggagtcgaa
     4621 ccaataccgg gtgattctgg aggccagtcc cgacgtccag aactcggtgg cctcgctgtc
     4681 caacatctac ttcccggccg cgggcggcgg acaggtgccg ttggcggcgc tggcgcgcgt
     4741 cgaaataaaa cccgtgccga tcgagaacaa ccatctcggc cagttccccg cggcgatggt
     4801 gtctttcgac gtggcgccga gctattcgct cggggccgcg gtcaaagcgg tgcagaaggc
     4861 ggaagcggac accggccttc ccgccagcat caacacctct tttcaaggct cggcgctggc
     4921 ttttcagtcc acgctcggca acgaattgct gctgatcctg gcggcgctgg tgacggtcta
     4981 tatcgtgctg ggcgtgctct atgagagctt
//
LOCUS       DASWPU010000029         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_55309,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000029 DASWPU010000000
VERSION     DASWPU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55309"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VGC27_00585"
     CDS             complement(<1..940)
                     /locus_tag="VGC27_00585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0281104.1"
                     /translation="MAKTALALMSDCHVLPTPDNFELFYVYASGENAALSRILGGMIA
                     QNAPFTPDALSELRARALIRERAAKTVEDVGSGINEMLDTVLGKLEAAGKHAGDYGRT
                     LSEMSGGLGGDHSPDGLRKLVDGLLGATRAMEERTKSLESELQQSSQQVSELKVQLDV
                     VRKETRIDPLTGIANRKAFDLELQAAIEDAIGSGDALTLLMCDIDHFKTFNDSWGHQT
                     GDQVLRLVANCLSENVKGRDTAARYGGEEFTVILRQTALGDAVKLANQVRSTVEGKKL
                     VKKSTGDILGRITISIGVAQLAARDHAETLVRRADTC"
     gene            complement(1078..2763)
                     /locus_tag="VGC27_00590"
     CDS             complement(1078..2763)
                     /locus_tag="VGC27_00590"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0281105.1"
                     /translation="MPQAEVPGLYTLTESAPAAPLTWPPKYRSSVPPAAEPAAPAQPE
                     EAATILVPDSAPVAGFDALGLSPDLLKAVAAAGYTTPTPIQAEGIPHVMKRRDIIGIA
                     QTGTGKTASFTLPMIELLSRGRAKARMPRSLILEPTRELAAQVAESFEKYGKFNKLSM
                     ALLIGGVSFDEQEKKLDRGVDVLIATPGRMLDHFERGKLMLSQVQILVIDEADRMLDM
                     GFIPDVERICKLIPFTRQTLFYSATMPAEIRRLTEQFLQNPVRVEVARPATTAENVTQ
                     ILIEVPNNDWAKREALRRLIGEAGEITNAIVFCNRKRDVDIVAKSLSKHGFNAGPLHG
                     DLDQSVRTKTLDSFRSGKLTFLVASDVAARGLDIPATSHIFNYDVPYHPDDYVHRIGR
                     TGRAGRSGSALMLATSRDAKYVEAIEKLTKMSLERRTLEGLAEAVEVRPPRDDRQGRD
                     RHGRDRNKSGRGRDRQHPVKPHGQVAAMEPVKADAATPAPRPQQVRPPKPQQQQQPRQ
                     RQPKPQQPREPQQPRESSQPDVNQLPAFLLRPVVLPKAPEKPARKPKKENPES"
BASE COUNT          488 a          961 c         1107 g          632 t
ORIGIN      
        1 ggcaggtatc ggccctccgc accagcgtct cggcatggtc ccgggcggcg agctgggcga
       61 cgccgatcga gatggtgatc ctgcccagga tgtctccggt cgatttcttt accagctttt
      121 tgccctcgac ggtggaacgg acctggttgg ccagcttgac cgcatcgccg agcgcggtct
      181 ggcgcaggat cacggtgaac tcctcgccgc cgtagcgcgc cgcggtgtcg cggcccttga
      241 cgttctccga caggcaattg gcgaccagcc gcagcacctg atcgccggtc tggtggcccc
      301 aggaatcgtt gaaggtcttg aagtggtcga tgtcgcacat cagcagcgtc agcgcgtcgc
      361 cgctcccgat ggcgtcttcg atggccgcct gaagttccag atcgaaagcc ttgcggttgg
      421 cgatgccggt caacggatcg atgcgcgttt ccttgcgcac gacgtcgagc tggaccttga
      481 gttcgctgac ctgctgggag gattgctgca actcgctttc caggctcttg gtgcgttctt
      541 ccatcgcacg ggtggcgccg agcaacccgt ccaccagctt gcgcagcccg tccggcgagt
      601 ggtcgccgcc caggccgccg ctcatctccg acagggtgcg gccgtaatcg cccgcgtgct
      661 tgccggcggc ttccaacttg cccaggaccg tatccagcat ttcgttgatg ccgctgccga
      721 cgtcttccac ggtcttggcg gcgcgttccc gtatcagggc gcgtgcgcgc aactcggaaa
      781 gggcgtccgg cgtgaacgga gcgttctggg cgatcatccc gccgaggatg cgcgagagcg
      841 cggcgttctc gcccgaggca taaacgtaaa acagttcgaa attgtcgggc gtgggcagca
      901 catgacagtc gctcatcagg gcgagcgcgg ttttcgccaa ggcttcttca cgttcctgac
      961 tatgcacctt tgcccccgag cttcgccgca gacccgtagc ggcagatttt cgaccgccgg
     1021 ttcgtaaaaa aacgttaagg gggcgacgcg gctcgcgcgg attattcaga aggtctctta
     1081 gctctccgga ttttctttct ttggtttacg cgccggtttt tcgggcgcct tgggcaggac
     1141 gaccggccgg agcaagaacg ccggaagctg gttcacatcg ggctgactgg actcgcgcgg
     1201 ctgctgcggt tcgcgcggtt gctgcggttt cggttgccgc tgtctcggct gttgttgctg
     1261 ctgcggcttc ggcggacgaa cctgttgcgg gcgcggcgca ggcgttgcgg cgtccgcctt
     1321 gaccggctcc atggcggcaa cctggccgtg cggcttcacc ggatgctggc gatcccgccc
     1381 gcggccgctt ttgttgcggt cccggccgtg ccggtcccgt ccctggcggt cgtcgcgcgg
     1441 cggacgcacc tcgaccgctt cggcgaggcc ttccagcgtg cgccgctcca agctcatctt
     1501 ggtcagcttc tcgatggctt ccacatattt cgcgtcgcgc gaggtggcga gcatcaaggc
     1561 cgatccgctg cgcccggcgc ggccggtgcg gccgatgcgg tgaacgtaat cgtcgggatg
     1621 atagggcacg tcgtaattga agatgtggct ggtggcggga atgtccagcc cgcgcgcggc
     1681 cacgtcgctg gcgaccagaa aggtcagttt gccggagcgg aagctgtcca gcgtcttcgt
     1741 gcgcaccgat tggtcgaggt cgccgtgcag cggcccggcg ttgaagccgt gcttcgacag
     1801 ggatttcgcc acgatgtcca cgtcgcgctt gcggttgcag aagacgatgg cgttggtgat
     1861 ctcgcctgcc tcgccgatca ggcggcgcag cgcctcgcgc ttggcccagt cgttgttcgg
     1921 aacctcgatg aggatttggg tgacgttctc ggcggtggtg gcgggacgcg ccacttccac
     1981 ccgcacgggg ttctgcagga actgttcggt caggcgacgg atttccgccg gcatggtggc
     2041 ggaatagaac aaggtctgcc gggtgaaggg gatcagcttg cagatgcgtt ccacgtcggg
     2101 gatgaagccc atgtccagca tgcggtcggc ttcgtcgatg acgaggatct gcacttgcga
     2161 cagcatcagc ttgccgcgct cgaaatggtc gagcatgcga ccgggcgtgg cgatcagcac
     2221 atcgacgccg cggtcgagct tcttttcctg ctcgtcgaac gaaacgccgc cgatcaagag
     2281 cgccatcgag agcttgttga acttgccgta tttctcgaag ctttcggcca cttgcgccgc
     2341 cagttcgcgc gtcggctcca ggatcagcga gcggggcatg cgcgccttgg cgcgtccgcg
     2401 cgacaaaagc tcgatcatcg gcagggtgaa ggaggcggtc ttgccggtgc cggtctgggc
     2461 gatgccgatg atgtcgcgcc gcttcatcac atgggggatg ccctcggcct ggatcggcgt
     2521 gggcgtggtg tagcccgccg cggcgacggc cttcagcaga tcgggcgaca gaccgagcgc
     2581 gtcgaagccc gcaaccgggg cagaatccgg aaccaggatg gtcgccgctt cctcgggctg
     2641 cgcgggcgcg gcgggttcgg ccgcgggcgg tacggatgac cggtatttcg gaggccaggt
     2701 caggggtgca gcgggggcac tttcggtgag cgtgtacagc cccgggactt ccgcctgggg
     2761 cacggtcgcc ggtgcgtcgc tgggcgagct ggactcggta gtttcagaag gaggtgaggc
     2821 gccggatgcc ggaggcggcc aatggaattc gagatcaggg gcgggggcag cagcctccgc
     2881 cgggccagct tgatcagggc tcgcattttc aggggaaagg ggtgcggcgg tggccgcctg
     2941 attaggtgaa gtatccgaag ggcttacttc aactgtaccg gcctgtgtgc cggcgttcgt
     3001 cacgttcgtc aaatacgctc ctcaaggagg gcgcattcaa gcgcccgcga ctacccgaat
     3061 tggcagccgg tttcctatat gcgccgtcat atggggaaca aaaggcgaaa agtcaaggtg
     3121 gaggggcgtt agaggatcag tttgaatttc ggtgagttag aaagtcgcgt ctgatccaga
     3181 tgaccaag
//
LOCUS       DASWPU010000030         6721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_18603,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000030 DASWPU010000000
VERSION     DASWPU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18603"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..485
                     /locus_tag="VGC27_00595"
     CDS             189..485
                     /locus_tag="VGC27_00595"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEY0281106.1"
                     /translation="MPQDNQTNPSEDEIRIRSYLIWEREGRPEGKAAEHWLQAKAELE
                     TEFEADWHAASLEGETTSFVLPHLPISSPPNRSVSAKIGPEPEAVPARAQSGKV"
     gene            complement(721..3240)
                     /gene="glgP"
                     /locus_tag="VGC27_00600"
     CDS             complement(721..3240)
                     /gene="glgP"
                     /locus_tag="VGC27_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY0281107.1"
                     /translation="MVTLPMPQLPDEISGLQSLALDLRWTWSHEGDALWTYVDEQLWK
                     RTHNPWTVLQSAPAERLKLLAADQKFRERLANFVTERQRYLQTPGWFRGTENASQLKG
                     VAYFSMEFGLGAALPLYAGGLGVLAGDFLKTASDLDVPIIGIGLLYQEGYFRQLVDAS
                     GMQQEFYPYNEPAAMPVEPVVLPEDGWLRIGLEMPGRTVQLRVWQANVGRVKLYLLDS
                     NDALNSPLDRGITAKLYGGGEEMRLMQEIVLGVGGWRVVEALHPEVEICHVNEGHAAF
                     AIIERARHLALKANLDFWTALWATRAGNVFTTHTPVAAGFDRFPADLLRKYLPYVEGA
                     LTGRGVKLEDVLALGRANPDDPNEPFNMAFLAQRGSAMSIAVSRLHGEVSRRIFQPLF
                     PRWPSCEVPITHVTNGVHIPTWDSAGADRIWTKAYGKERWRSGVVESGERIEAVSDDE
                     LWAMRGEGRQRVVHIARQHLARQLRERGAGLEAIQIADTVLDPNVFTLGFARRFTEYK
                     RPNLLLRDLKRFDDLLHNDMLPVQIVVAGKAHPADTVGKEMIQEWIKLARQPRYRRHV
                     VFLEDYDISVAQDLVQGVDVWINTPRRPWEACGTSGMKVLVNGGLNCSTLDGWWDEAY
                     EPSVGWLIGDGIGGAAEEVDARDAASLYDVLESKIIPEFYDRDADGLPRVWLSRIRES
                     MGRLTPGFGGARMMRDYVEAYMPLSKAVRSRLKDGCALAKAMNQWARTLHGRWQGLHI
                     GSAIANQTDSTWHLSVPVYMGEVSPESVRVELFADATAEYAPEVVLLHQDHAIAGSAN
                     GYIYAGALNSSRPLKDFTVRVVPYHAGAFLPAELPLIAWQH"
     gene            complement(3559..4875)
                     /gene="xylA"
                     /locus_tag="VGC27_00605"
     CDS             complement(3559..4875)
                     /gene="xylA"
                     /locus_tag="VGC27_00605"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY0281108.1"
                     /translation="MSLFSDVAPIRFEGPDSTNDFAYKVYDPNRQVLDKRMADWLRLT
                     VCYWHSFSASGMDTFGSGTWPRPWLGGAIDQKVAEQRAEAAFDFISRLGTPFFAFHDA
                     DVIADSSSLKEQIDNLKKMEDVFAKKMAETKVKLLWGTANLFGPPRFAAGASTNPNPE
                     VVAWCAAQVKAILETTHRLGGENYVLWGGREGYDTLLNTDLKRELEHMGRFLTMVVEH
                     KHKIGFKGTILIEPKPFEPTKHQYDRDVATIYGFLLKNGLEKEVKVNVEVNHATLAGL
                     DFEHELAVANVHGILGSLDINRGDPRNGWDTDQFPNNAQDLVPAMLEIIKAGGLGTGG
                     CNFDAHLRRQSVDAVDLYIAHITGIDTLARALLAAEVVVKQGKLEAIRKARYAGWDAE
                     LGQKIEKGEFTLESLADYALKHAVDPKPQSGKQELCEGIIAGACKF"
     gene            complement(4872..6332)
                     /gene="xylB"
                     /locus_tag="VGC27_00610"
     CDS             complement(4872..6332)
                     /gene="xylB"
                     /locus_tag="VGC27_00610"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEY0281109.1"
                     /translation="MFLGIDVGTSSVKAVLVDGNDRVAAQASAPLTVERPQPLWSEQD
                     PDSWWRATVEAVRALPEKERSAVKAIGLSGQMHGATLLDKSDKPLRPAILWNDGRSEQ
                     ECQELERLEPRSREITGNLAMPGFTAPKLLWVRRHEPDIFSRTQTVLLPKDYVRLKLT
                     GEKASDMSDAAGTLWLDVGKRDWSDAMLQATGLKRANMPRLFEGNAPTGKLTADAARQ
                     LGIPAVVVAGGGGDNAASAVGLGVVKTGEAFLSLGTSGVLFVVTDKFRPNPAKAAHAF
                     CHAIPARWHQMSVILSAAANLDWVMQLCGFKDIPDTFHAAEAKGLHKQSPIFLPYLSG
                     ERTPHNDPCARGVFFGLSAETDKADLAYAVLEGVAFAFADGLDVLVEAGSAIGDISMT
                     GGGARIPYWGRLLASALDKPLTYREGGEVGAAYGAARLGRLALTGEDPDKVCVAPPVT
                     RIVEPEAAFKEQMARRRPLFVNLYRNLKTSFREFAR"
     gene            complement(6538..>6721)
                     /locus_tag="VGC27_00615"
     CDS             complement(6538..>6721)
                     /locus_tag="VGC27_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0281110.1"
                     /translation="TAAMVLGVMPLIMASGAGAAARYNLGLVIATGIAIGTCFTLFVV
                     PAIYLLIGRTHTKETG"
BASE COUNT         1296 a         2102 c         2079 g         1244 t
ORIGIN      
        1 cgtggaagag acccgcgcca aggcggcggc gcaagaaaca gcaagacaaa tcgcggggtg
       61 acgcgctatc atcgcggcat ggattgcggc tccgttaacg agttgttttt caaccgctag
      121 gtatggaatc attcacaaaa cgtgaatgaa gtatgaccgt tggtgcagac gaagggcggg
      181 agagtgccat gccccaagac aaccagacca atccttccga ggacgaaatc cgtatacgca
      241 gctatctgat ctgggagcgc gaaggccgcc cggaaggcaa ggcggcggaa cattggttgc
      301 aggccaaggc ggagttggag acggaattcg aggcggactg gcatgccgcc tcgctcgagg
      361 gcgaaacgac gagtttcgtt ttgcctcacc tgccgatttc ttcgccgccg aacagaagcg
      421 tatccgccaa gatcggcccg gaaccggaag ccgttcccgc tagagcgcaa tccggcaaag
      481 tgtgaagcgg tttgccgtca gattgcgcga aaaccaagga atctagagct tgatccgatt
      541 caatcaaatc ggagcaagct cgagtgtccc gattccgaaa ttcgctctct atatatcggc
      601 ctcgggcgaa tttcggaatc gaaaggacac tagcaaccta ttgaatccta gtgtcgtttt
      661 ggtttcgaag ttcgcttgca gccagatata acgaatgctg cgaacttcga aaccacgaca
      721 ctagtgctgc caagcgataa gcggcagttc ggcaggcaag aacgcgcccg cgtgatacgg
      781 cacgacgcgg accgtaaagt ccttcagcgg acgagacgaa ttcaacgcac cggcgtagat
      841 gtagccgttg gccgatcccg caatggcgtg gtcctgatgc agtagaacca cctcgggcgc
      901 gtactccgcc gtggcgtcgg caaacagttc gacgcgcacg gactccggcg aaacctcgcc
      961 catatagacc ggcaccgaca gatgccatgt cgaatcggtc tggttggcga tcgcacttcc
     1021 gatgtgcagg ccctgccagc ggccgtgcag ggttctcgcc cattggttca tggccttggc
     1081 caaggcgcag ccgtccttca gccgggaacg caccgccttg gagagcggca tataggcttc
     1141 gacatagtcg cgcatcatgc gcgcgccgcc gaaccccggc gtcagcctgc ccatgctttc
     1201 gcgaatccgg ctcaaccaga cgcgcggcaa accgtcggcg tcgcgatcgt agaattccgg
     1261 aatgatcttg ctttcgagaa catcatagag gcttgccgcg tcgcgggcat cgacctcctc
     1321 cgccgcgccg ccgatgccgt cgccgatcaa ccagccgacg gagggttcat aggcctcgtc
     1381 ccaccagccg tcgagcgtcg agcaattgag gccgccattg accagcactt tcatgccgct
     1441 ggtgccgcag gcttcccagg gccggcgcgg cgtgttgatc cagacgtcca cgccttgcac
     1501 cagatcctgc gccaccgaga tgtcgtaatc ctccagaaac acgacatggc gccggtaacg
     1561 cggctggcgc gccagcttga tccattcctg gatcatctcc ttgccgacgg tgtcggccgg
     1621 atgcgccttg ccggcaacga caatctggac cggcagcata tcgttgtgga gcagatcgtc
     1681 gaagcgcttg aggtcccgca acagcagatt ggggcgcttg tactcggtga accggcgcgc
     1741 gaagccgagc gtgaagacgt tgggatcgag tacggtgtcg gcgatctgaa tggcttccag
     1801 tccggcgccg cgttccctta gctgcctggc cagatgctgg cgcgcaatat gtacgacgcg
     1861 ctggcgtcct tcgccgcgca tggcccacag ctcgtcgtcc gaaacggcct cgatccgttc
     1921 gccgctttcg accacccccg aacgccagcg ttccttgccg taggccttgg tccaaatcct
     1981 gtcggctccg gccgaatccc aggtcgggat atgcacgccg ttggtcacat gcgttatcgg
     2041 cacttcgcag gaaggccagc ggggaaacag cggctggaag attcgccggc tgacttcgcc
     2101 gtgcaggcgg ctgacggcga tgctcatggc cgaaccgcgc tgcgccagga acgccatatt
     2161 gaacggttcg ttaggatcgt ccggattggc gcggccaagc gccaggacgt cttccagctt
     2221 gaccccgcgc ccggtcagcg ccccttccac atagggcaga tatttgcgca gcagatcggc
     2281 cggaaaacgg tcgaacccgg cggctacggg ggtatgggtg gtgaagacgt tccccgccct
     2341 ggtggcccac agagccgtcc agaaatcgag attggccttc agcgccagat ggcgcgcccg
     2401 ctcgatgatc gcgaacgctg catggccttc gttgacatgg cagatttcaa cctcgggatg
     2461 aagcgcttcc accacgcgcc agccgccgac gccgaggacg atctcctgca tcagacgcat
     2521 ttcttcgccg ccgccgtaga gctttgcggt gatgccgcgg tccagcgggc tgttgagcgc
     2581 gtcgttgctg tccagcagat aaagcttcac gcggcccaca ttggcctgcc agacgcgcaa
     2641 ctgcacggtg cggccgggca tttcgagccc gatgcgcagc cagccgtctt ccggaagcac
     2701 gaccggctcg acgggcatcg ccgccggttc gttgtaggga tagaactcct gctgcatgcc
     2761 gctggcgtcg acgagctgcc ggaaatagcc ttcctgataa agcagcccga tgccgatgat
     2821 gggaacgtcg aggtcgctgg ccgtcttgag aaaatctccg gcgagaacgc ccaatccgcc
     2881 ggcataaagc ggcaaggccg cgcccaaacc gaattccatg ctgaaatagg cgacgccttt
     2941 cagctgcgac gcgttctcgg tgccgcgaaa ccagccgggc gtctgcaaat agcgctgccg
     3001 ttcggtgacg aagttcgcaa gcctttcgcg aaatttctgg tcggccgcca gcagtttgag
     3061 gcgctccgcg ggagcgcttt gcagcacggt ccaaggatta tgggtgcgct tccaaagctg
     3121 ctcgtccaca taggtccaca aagcgtcgcc ttcgtgactc catgtccagc gcaaatcgag
     3181 cgccaacgac tgcaaccctg aaatttcatc cggcaattgc ggcataggaa gcgtcaccat
     3241 gatctcgatc cgtaaactct ctttgttgca gggccgccgt tcgtttggcg ccgggttttc
     3301 gttcggagaa tcatcacata agcgggtaag ttaattgaac ttaattaaaa agtccctgtt
     3361 gcgggggcgt gcgaaggctg cggcgccggt ttggcgaata tccatgaaag gcgcggaaca
     3421 aattcccctg cccgccacta tgcggtccgt ttcctgaaat ttactatttg cacaatcctc
     3481 cgcgcgcaac cccaaaaaac atggccaccc tcacccagat tgaaaaggcg agaatggcca
     3541 atgctagtgt ttcgccgttt agaacttgca ggctccggcg atgatgccct cgcacagttc
     3601 ctgcttaccc gactgcggct tgggatcgac ggcgtgcttc agcgcgtagt cggcgaggga
     3661 ctccaaagtg aattcgccct tttcgatctt ctggccaagc tcggcgtccc aacccgcgta
     3721 gcgggccttg cggatcgctt ccagcttgcc ttgcttgacg acgacttcgg cggccagcaa
     3781 cgcgcgggcc agcgtatcga tgccggtgat gtgggcaatg tagagatcta ccgcatcgac
     3841 cgactggcgg cgcagatggg cgtcgaagtt gcagccgccg gtgcccagac cgcccgcctt
     3901 gatgatctcc agcatcgccg gaaccaggtc ctgcgcgttg ttggggaact ggtcggtatc
     3961 ccagccgttg cgcggatcgc cgcggttgat atcgagcgag ccgagaatgc cgtggacgtt
     4021 ggccaccgcc agctcatgtt cgaagtcgag gccggccagc gtggcgtgat tgacctcgac
     4081 attgaccttc acttcctttt ccaggccgtt cttgagcagg aagccgtaga tggtggcgac
     4141 atcgcggtcg tattgatgct tggtcggctc gaacggcttg ggctcgatca ggatggtgcc
     4201 tttgaagccg atcttgtgct tgtgctcgac caccatcgtg aggaagcggc ccatatgttc
     4261 gagctcgcgc ttcaaatcgg tattgagcag cgtgtcgtag ccttcgcggc cgccccacag
     4321 cacatagttc tcgccgccga gacggtgggt ggtctccagt atcgctttga cctgcgcggc
     4381 gcaccaagcg acgacttccg ggttcgggtt ggtcgaggcg ccggcggcga agcgcggcgg
     4441 cccgaacagg ttagcggtgc cccacaacag cttcaccttg gtctcggcca tcttcttggc
     4501 gaagacgtct tccatcttct tgagattgtc gatttgttcc ttcagcgaag acgaatcggc
     4561 gatcacgtcg gcgtcgtgga aggcgaaaaa tggcgtgccc agacggctga tgaagtcgaa
     4621 cgcggcctcg gcgcgctgtt cggcgacctt ctggtcgatg gcgcctccga gccacgggcg
     4681 cggccaggtt cccgaaccga acgtgtccat gccggacgcg ctgaaggaat gccagtagca
     4741 gaccgtcagg cgcagccaat cggccatgcg cttgtccagg acttggcggt tcgggtcata
     4801 gactttgtag gcaaaatcgt tggtgctgtc cggcccttcg aagcggatcg gagcgacgtc
     4861 ggaaaagagg ctcatctggc gaactccctg aaagatgtct tgaggtttcg gtaaaggttg
     4921 acgaaaagcg gccggcggcg cgccatctgc tctttgaagg ccgcctcggg ttcgacgatg
     4981 cgcgtaaccg gcggggcaac acaaaccttg tcgggatcct cgccggtcag cgccagccgg
     5041 ccgaggcggg cggcgccgta agcggcgccc acttcgccgc cttcacgata ggtcagcggc
     5101 ttgtcgagtg cagaggcgag cagacggccc caatagggaa tgcgcgcgcc gccgccggtc
     5161 atcgagatat cgccgatagc gctgccggct tccaccagca cgtccagccc gtcggcgaag
     5221 gcgaaggcga cgccttccag caccgcatag gcgagatccg ccttgtcggt ttcggcggaa
     5281 agcccgaaga acacgccgcg cgcgcacgga tcgttatgcg gggtgcgctc accggaaaga
     5341 tagggcagga agatcggcga ctgcttgtgc aggcccttgg cttccgccgc gtggaaggtg
     5401 tcggggatgt ccttgaaacc gcaaagctgc atcacccagt cgaggttggc ggcggcggaa
     5461 agaatgaccg acatctgatg ccaacgcgcc ggaatggcgt ggcagaaggc atgcgccgcc
     5521 ttggccgggt tgggccggaa cttgtcggta accacgaaca gcacgcccga agtgccgagc
     5581 gacagaaacg cctcgccggt cttcaccacg ccaaggccca ccgcgctggc cgcgttgtcg
     5641 ccgcccccgc ccgccacgac gacggcggga atccccaact gccgcgccgc gtcggccgtc
     5701 agcttgccgg tcggcgcatt gccttcgaac agacgcggca tgttcgcgcg cttcagcccg
     5761 gtcgcctgca acatcgcgtc ggaccagtcg cgcttgccga catcgagcca cagcgttccg
     5821 gccgcgtccg acatgtcgct ggccttctcg cccgtgagtt tgagacggac ataatccttg
     5881 ggcagcaaaa ccgtctgtgt acggctgaaa atatccggct cgtggcggcg gacccaaagc
     5941 agtttgggcg cggtgaaccc aggcatggcc agattgccgg tgatctcgcg cgagcgcggt
     6001 tcaagccgtt cgagttcctg gcattcctgt tccgagcgcc cgtcgttcca gagaatcgcg
     6061 gggcgcagcg gcttgtccga cttgtcgagc aaggtggcgc catgcatttg cccgctcaga
     6121 ccgatggcct tgacggcact gcgttccttt tcgggcagag cgcgcacggc ctcgaccgtc
     6181 gcgcgccacc aggaatccgg atcctgctcc gaccacaaag gctggggccg ctccaccgtc
     6241 aacggcgcgg aggcttgcgc cgccacccgg tcgttgccat cgaccagaac ggccttcaca
     6301 cttgatgtgc caacatcaat cccgagaaac atgacgcttc ctgtccctga attttcatct
     6361 tgtcgccaag ggacggtacc caggtcttcg cggcccgccg ccagcgccgt ttggtagcgc
     6421 tatcatatag cagcccacgc cgaggtaaac ctagaaatcg ccgctcagaa gcctagagaa
     6481 aggccgggat ttcccggcct gtcaagcggc caagcagcgc atccgggccg gcgcggccta
     6541 gccggtctct ttggtgtggg tgcggccaat aagcaggtag atcgccggta cgacgaaaag
     6601 ggtgaaacac gtcccaatgg cgatacccgt ggcgatgacc aggcccaaat tgtagcgcgc
     6661 cgccgctccg gcgcccgagg ccatgatcag cggcatgacg cccagcacca tggcggccgt
     6721 g
//
LOCUS       DASWPU010000031         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_98048,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000031 DASWPU010000000
VERSION     DASWPU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98048"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VGC27_00620"
     CDS             complement(<1..327)
                     /locus_tag="VGC27_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY0281111.1"
                     /translation="MSTQILMPALSPTMEEGKLAKWLVKEGDTVKSGDILAEIETDKA
                     TMEFEAVDEGRIGKILVPEGTEGVKVNSPIAELIGEGEDSSSSASPLEGEAVLGARAR
                     ATETVGG"
     gene            complement(327..1334)
                     /gene="pdhA"
                     /locus_tag="VGC27_00625"
     CDS             complement(327..1334)
                     /gene="pdhA"
                     /locus_tag="VGC27_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY0281112.1"
                     /translation="MSDPTKDVVAGVNTAVPVANSMLKTFYSDMLLIRRFEERAGQLY
                     GMGLIGGFCHLYIGQEAVVVGMQANVGPQDQVITAYRDHGHMLACGMDPKGVMAELTG
                     RAAGYSRGKGGSMHMFSAEKRFFGGHGIVGAQVPLGTGLAFADRYKGNGGVTLNYFGD
                     GAANQGQVYESFNMAELWKLPVVYIIENNQYAMGTSVERSSAQPQFFKRGASFNIPGE
                     EVDGMDVEAVYEAGRKAVEWCRSGKGPFILEMKTYRYRGHSMSDPAKYRTREEVQTVR
                     ERRDPIEQLGQKLIARKIATEDELKAIDKETRLIINTAAEFAIETPEPDPSELFTDVV
                     A"
     gene            complement(1446..1751)
                     /locus_tag="VGC27_00630"
     CDS             complement(1446..1751)
                     /locus_tag="VGC27_00630"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY0281113.1"
                     /translation="MTRIFGLAVLPAISAAVISYFGYHGIWGERGYLALNDTRTRIAA
                     ADRQLDQISDQNRRLEHRIMLLKAGSADPDLVEELARTQLMDGAPGQVAVPRAAPLQ"
BASE COUNT          337 a          629 c          625 g          424 t
ORIGIN      
        1 accccccacc gtttcggtcg cgcgtgcccg cgcgcccaaa accgcctccc cctcaagagg
       61 ggaggctgaa cttgaactgt cctcgccctc gccgatcagt tcggcgatgg gagaattcac
      121 cttgacgcct tcggtgcctt cgggaaccag gattttaccg atgcggcctt cgtccaccgc
      181 ttcgaattcc atcgtcgcct tgtcggtctc gatctcggcc aggatgtcgc cggatttcac
      241 cgtatcgcct tccttgacca gccacttggc gagcttgccc tcttccatgg tgggcgacag
      301 ggcgggcatc aaaatctggg tggacatcag gcaaccacgt cggtgaagag ttcggacgga
      361 tcgggttccg gggtttcgat ggcgaattcg gcggccgtgt tgatgatcag gcgggtttcc
      421 ttgtcgatgg ccttcagctc gtcttccgtg gcgattttgc gcgcgatcag cttctggccc
      481 agctgttcga tgggatcgcg ccgttcgcgc acggtctgga cttcctcgcg cgtgcggtat
      541 ttggcggggt ccgacatgga atggccgcga tagcgatagg tcttcatttc cagaatgaag
      601 gggcctttgc cggagcggca ccattccacc gccttgcgtc ccgcttcgta aacggcttcg
      661 acgtccattc cgtccacttc ctcgcccgga atattgaaag aggcgccgcg cttgaagaat
      721 tgcggctggg cgctcgagcg ttcgacgctg gtgcccatgg cgtattggtt gttctcgatg
      781 atgtagacga ccggcagctt ccatagttcg gccatgttga agctctcgta tacctgacct
      841 tggttggccg cgccgtcgcc gaaatagttg agcgttacgc cgccattgcc cttgtagcgg
      901 tcggcgaagg ccagcccggt gccgagcggc acctgcgcgc cgacaatgcc gtgaccgccg
      961 aagaacctct tctcggccga aaacatatgc atcgagccgc ccttgcccct ggaatagccg
     1021 gcggcgcggc ccgtcagctc cgccatcacg cccttggggt ccatgccgca ggcgagcata
     1081 tggccgtgat cgcgataggc ggtgatgacc tggtcctgcg gcccgacatt ggcctgcatg
     1141 cccacgacca cggcctcctg gccgatatag aggtgacaga aaccgccgat cagccccatg
     1201 ccgtaaagct ggcctgcccg ttcctcgaac cggcggatca gcagcatgtc ggaatagaag
     1261 gttttcagca tcgaattcgc gaccggcacg gcagtgttaa caccggctac aacatccttc
     1321 gtcggatcgc tcatttcccg gcccactttg cccgtacgtt tgaggagcgg gcgttgtggc
     1381 aagcgcgccc gcattttgca agcttaacgg catatccggg cattgttttg gcggcggact
     1441 gggggctatt gcagcggtgc cgccctggga accgccactt ggccgggggc gccgtccatc
     1501 aactgggtac gggccagctc ctcgaccagg tcgggatcgg ccgagccggc cttgagcagc
     1561 atgatgcggt gttcgagccg ccggttctga tcgctgatct ggtccagctg gcggtccgcg
     1621 gcggcgatcc gggtcctggt gtcattgagc gccagataac cgcgttcacc ccagatcccg
     1681 tgatagccga aataggaaat cacggcggcg ctgatggctg gcaggaccgc cagaccgaag
     1741 attcgcgtca cgcttcgcct gattcgcatg actggaacga atcacgactg attcgggtgg
     1801 tcaagcgaag aatggttaag actgcaacca tggcggccga tatgcccgta ccggccccga
     1861 cgttacgcgc aggaacggcg attgccgcgc cccaacctct gcgttaatgg acgttaagct
     1921 tgtccacgga ggcgatcgcc ttgaggggta ttgctcctcg tgccgcagtg ttcgggatcg
     1981 gggcgccgcg ctccgattgg ttcggtgcca tgctt
//
LOCUS       DASWPU010000032         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_85772,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000032 DASWPU010000000
VERSION     DASWPU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85772"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VGC27_00635"
     CDS             complement(<1..191)
                     /locus_tag="VGC27_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281114.1"
                     /translation="MSASTSFQTYDQVAGAFVGIIPGETRLSDLPKLGFDTATTPNVE
                     ILSYLGVIERFMPRNSLTF"
     gene            complement(430..1416)
                     /locus_tag="VGC27_00640"
     CDS             complement(430..1416)
                     /locus_tag="VGC27_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY0281115.1"
                     /translation="MAVKPPTFADIEAAAARIAPFAFRTPLVENAGLNRQLGGRVLLK
                     LETLQRTGSFKFRGALNRIGMIPPDARGRGVVAFSSGNHAQGVAAAAALFGVPALIVM
                     PKDAPRAKLEGTKAYGAEIVFYDRVRDDREAIALALCKERGSTLVRPFDDAGVVAGQG
                     TVGLEIAQDAAARGVTLDAVLAPCSGGGLIGGIALAFSVLSPATRTVSVELDGYDGMR
                     RSLAAGARIAAPGGALSIGDGLMAPMPGEIPFALAQLHLTDTLAVGDAELVSAVAFAA
                     RILKLIVEASGVAALAALISGKYAVRDKAVAVVLTGANVDFSTIADACARTR"
BASE COUNT          317 a          522 c          474 g          297 t
ORIGIN      
        1 tcgaaggtca ggctgttgcg cggcatgaag cgctcgatga cgccgagata ggaaaggatc
       61 tcgacattcg gcgtcgtcgc cgtatcgaag ccgagcttcg gcaggtccga cagacgcgtc
      121 tcgccgggaa tgattccgac aaaggcgccc gcaacttgat cgtaggtctg gaaactcgtg
      181 cttgccgaca ttcgggattc gtgcggcaga agtcccagcc cgccgcaccc cacgagcgac
      241 gacatgaacg atgctatgac gattattttc gctgtacggc gaggtccgcc gtccgccccg
      301 ttttgcatgt gccccaaccc accaaaaccc acggggggaa tttaccttcc cctcttcttg
      361 aggttagcgg tctttgcgga atttggaaag gagcgcggca ctttatcgtg actatacgcc
      421 gcgtcatggt tacctggttc gggcgcacgc atcggcgatc gtggaaaagt caacattggc
      481 tccggtcagc accacggcga cggctttgtc ccttaccgca tatttgccgg aaatcagggc
      541 cgcaagagcc gcgacgccgc tcgcttccac gatcaatttc agtattcgcg ccgcgaaagc
      601 gaccgcgctc accagctccg catcgccgac ggccaatgtg tcagtaagat gaagttgtgc
      661 aagtgcgaag ggaatttcgc ccggcatcgg cgccatcaga ccgtcgccga tcgagagcgc
      721 gcctccgggc gcggcaatac gcgcaccggc cgccaacgat cttctcatgc cgtcatagcc
      781 gtcgagttcg acgctgacgg ttcgcgtcgc cggactcaag acggagaacg ccagcgcgat
      841 tccgccgatc aagcctccgc cgctgcacgg cgccaggacg gcatcgagcg ttacgccgcg
      901 cgcggccgca tcctgcgcaa tctccaaacc caccgtgcct tgccccgcca cgacgccggc
      961 atcgtcgaac ggccgcacca gagtggagcc gcgttccttg cacagcgcca gcgcgatcgc
     1021 ttcgcggtcg tctctgaccc ggtcatagaa gacgatctcg gcgccatacg ccttggtgcc
     1081 ctccagtttg gcgcgcggcg catccttggg catgacgatc agcgccggta cgccgaacag
     1141 cgcggcggcc gccgccacgc cttgcgcatg attgccggag gagaacgcca ccacgcccct
     1201 gccgcgcgcg tccggcggga tcatgccgat gcggttaagg gcgccgcgga atttgaacga
     1261 gccggtccgc tgcaaggtct ccagcttcaa cagcacccgc ccgcccagtt gccggttgag
     1321 gccggcgttt tccaccaaag gagtcctgaa ggcgaacggc gcaatgcgcg cggcggcggc
     1381 ttcgatatcg gcaaaggttg gcggcttgac agccatgtta taactccggt gaacgaaacc
     1441 caatctagtg tcccgattcc gaaattcgct gcatctctat atcggcgtcg ggcgaatttc
     1501 ggaatcgaaa ggacactagc aacctattga atcctagtgt cgttttggtt tcgaagttcg
     1561 cttgcagccc gatataacga atggtgcgaa cttcgaaacc acgacactag
//
LOCUS       DASWPU010000033         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_55331,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000033 DASWPU010000000
VERSION     DASWPU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55331"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VGC27_00645"
     CDS             <1..283
                     /locus_tag="VGC27_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0281116.1"
                     /translation="LILGTLAAGPRRFSAVQRAIPDISKRMLTQTFRDLERDGLIART
                     VFPTKPPSVEYRLTPLGATILEPLASLIRWANRSHAAIKDARLAFDEAV"
     gene            370..1327
                     /locus_tag="VGC27_00650"
     CDS             join(370..705,707..1327)
                     /locus_tag="VGC27_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612137.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY0281117.1"
                     /translation="MTRPYSEDIRERALSRADAGETVRSIAEALQISPSCVTKWKNLR
                     RATGSLSPGKIGGHKKRVLSDANADWLRKRVRSGPFTLRKLTQELAARGIKTDVRAVW
                     TFVHTEGLSFKKTIHPTEQDRPDVARKRTRWKAHQGRIEASRLVFIDETWIKTNMAPL
                     RGWGPCGQRLQASAPFGHWKTMTFIAALRHDRISAPWIIDSPINGELFTLYVEKVLAP
                     TLTRGEVVILDNLGSHKGKLARDAIRAKGAHLFFLPPYSPDLNPIEQVFVEQVFAKLK
                     HLIRAAEPRDVEATWRKVGELLNLFSKDECANYLKNSGYVSV"
     gene            complement(1489..>1675)
                     /locus_tag="VGC27_00655"
     CDS             complement(1489..>1675)
                     /locus_tag="VGC27_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281118.1"
                     /translation="GFLGGPTPVSVQTVLFMSKTLTMKRFSNFESATVKDPTRLASAL
                     RSGGTDRGPFVQNKYRA"
BASE COUNT          348 a          520 c          493 g          314 t
ORIGIN      
        1 gctgatactg ggcactctcg cggcggggcc gcgccgattc agtgccgtcc agcgcgcgat
       61 cccggacatt tccaagcgga tgctgacgca gactttccgc gacctggagc gtgatggcct
      121 gatcgcgcgc acggtattcc cgaccaagcc gcccagcgtc gagtaccggc tgacccctct
      181 tggcgccacg atccttgaac cgctggcttc ccttattcga tgggcaaatc ggagccatgc
      241 cgcaattaag gacgcccgct tggcgttcga tgaggccgtc tagagcatgt tgtttttgcc
      301 tggaatcggg attcccagta ggggcgattt ctgattcaag ctccctgctg ggtacggagg
      361 ccagcatgga tgacgcgacc ctattcagaa gatatccgcg aacgcgcttt gtcgcgagct
      421 gacgcggggg agacggttcg ttcgattgca gaagcgctac agatcagtcc ctcctgcgtg
      481 acgaagtgga agaatctgcg gcgggctacc gggagcctgt cgcctggcaa gatcggcggc
      541 cacaagaaac gggttctgtc ggatgccaat gccgactggc tacgcaagcg cgttcgctcg
      601 gggccattca ccttgcgcaa gctgacccag gaactggccg cacgcgggat caagacagac
      661 gtgcgagcgg tgtggacttt tgttcacacc gaggggctga gcttcaaaaa aaacgatcca
      721 tcccaccgaa caagaccgtc ccgacgtcgc ccgtaaacgg acccgctgga aggcgcatca
      781 aggcaggatc gaggcttcgc gcctggtgtt tatcgacgag acctggatca aaaccaacat
      841 ggcgccgctg cgcggctggg gaccttgtgg gcagcgcctc caagcatctg caccgttcgg
      901 ccattggaag accatgacct tcatcgcggc gctgcgtcac gaccggatca gcgcgccctg
      961 gatcattgac agccccatca atggcgagct cttcacgctc tatgtcgaga aggtgctggc
     1021 gcccaccctg acaaggggcg aggtcgtcat cctcgacaac ctcggcagtc acaagggaaa
     1081 gctcgcccgc gacgcgatcc gcgccaaggg ggcgcatctg ttcttcctgc cgccttacag
     1141 ccccgacctc aatccgatcg agcaagtttt cgtcgagcag gttttcgcca aactcaaaca
     1201 cctgatccga gccgccgagc ctcgtgacgt cgaggcaacc tggcgaaagg tcggcgaact
     1261 cctcaatctc ttctccaagg acgagtgcgc caactatctt aaaaactcag gctatgtttc
     1321 cgtataaaaa catcatgctc taaggcccga tgcatggtgg cgatgtccgc attacgcatc
     1381 gggctgcgcc tgttcgacgc cttgaggcaa ggtcaggaaa cgagcacggc cttggcgccc
     1441 ccggccggag cgtaacggag cgccgcttcg atgtcctcaa agccgaactc atgcccgata
     1501 tttgttctga acaaagggtc cgcgatcagt ccctccagat cggagcgcag aggcaagccg
     1561 ggtggggtct ttcacggtag cgctctcgaa attactgaac cgcttcatcg tcagggtctt
     1621 cgacatgaac agcacggttt gaaccgacac cggagtcggg ccaccgagaa acccg
//
LOCUS       DASWPU010000034        13467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_116420,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000034 DASWPU010000000
VERSION     DASWPU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 13467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13467
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116420"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VGC27_00660"
     CDS             <1..402
                     /locus_tag="VGC27_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281119.1"
                     /translation="GLGFLMLVFGSNYHNAVFRVPLYWLVFAYFLHSMGELCLSPVGL
                     SMISKLSVQKMVGMMFGVWLLSSAMAQYVAGIIAQLASTETVAGKVLNAQVSLETYLG
                     VFQTIGIAGILSGCVLLALWPILKRGMHGIH"
     gene            485..1645
                     /gene="metC"
                     /locus_tag="VGC27_00665"
     CDS             485..1645
                     /gene="metC"
                     /locus_tag="VGC27_00665"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEY0281120.1"
                     /translation="MTDERDKPETIAVSAGRMSEAHFGTVNTPVYRTSTILHPDVASL
                     KSHDAPYSYGRRGTPTTRSFEEAMEALEGAARTVSLPSGLNAIATAVLSVCRAGDHLL
                     MVDSCYGPTRHLCGTVLKRLGVETTYYDPLIGEGIAALLRPNTRAVYCESPGSLTFEV
                     QDVPAIAAAAHAKGASVLMDNTWGTPLYFPALARGVDLSIHAATKYICGHSDTMMGTV
                     SANQSHAARLGNFVGDLGLYASGDDCFLALRGLRTLAVRLKRHQETALTLSRWLLGRP
                     EVSRVLYPALENDPGHALWKRDFLGASGLFGVVLKPVGEKSVTAMIDGLKHFGIGYSW
                     GGYESLIIPADFKRTAKAFEAEGPVIRIHAGLEDVDDLRADLEAGFVRLNAS"
     gene            1642..1983
                     /gene="hisI"
                     /locus_tag="VGC27_00670"
     CDS             1642..1983
                     /gene="hisI"
                     /locus_tag="VGC27_00670"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY0281121.1"
                     /translation="MTDTISHEAFLNAVKFNGDGLVPVIAQSHATGAVLMLAWMNRHT
                     LEETLSTGQVTYWSRSRGKVWRKGETSGNTQRLVEAWIDCDGDTLLLKVDQLGPACHT
                     GAPSCFFRKLG"
     gene            complement(1980..3284)
                     /locus_tag="VGC27_00675"
     CDS             complement(1980..3284)
                     /locus_tag="VGC27_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEY0281122.1"
                     /translation="MKLVGGRWSNWSGGVWCRPRTVVAPRDEVELAAAVRKAEDVVRV
                     PGGGHSFSPLNATSGTLIDLKAFNGLLGFDPERQVATIAAATPLWEVGPLLRPAGCGL
                     KNMSDIDRQTLAGAVSTGTHGSGIALGSYSAEVAGFRLVKANGEVIDCSANENADIFA
                     AGRTSLGTFGVITEIAMNVRPTYKLVKKEFSHSIGELFRQLDGLVAANRHFKFFWFPY
                     SDTAVCKSLNETEANARAPRSAEKLRARGERVSIEDDVFTAVNDALPRAPLLMKPAHR
                     LFSQLMPGRQHVRWSHEILPSPRSVRFNEMEYAVPYEKGPEVLREIVETIRKRQIKTG
                     FPIEYRTVAADDVWLSPFYRRKSATIAVYQYHRVDATALFEACEAVFRRYDGRPHWGM
                     RHTLTARELADLYPQYGAFRALRAKLDPAGKFLNEHLRGLFG"
     gene            complement(3396..3472)
                     /locus_tag="VGC27_00680"
     tRNA            complement(3396..3472)
                     /locus_tag="VGC27_00680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3436..3438),aa:Arg,seq:tct)
     gene            complement(3490..3795)
                     /locus_tag="VGC27_00685"
     CDS             complement(3490..3795)
                     /locus_tag="VGC27_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEY0281123.1"
                     /translation="MRARIYMPAKSAMQSGKARTKAWVLEHEPVSPRRIDALMGWTGS
                     TDMLSQVQLEFDSKEEAIAYAERHGIAFDLFEPHRPVPKPKAYADNFRYDRKVPWSH"
     gene            complement(3916..3992)
                     /locus_tag="VGC27_00690"
     tRNA            complement(3916..3992)
                     /locus_tag="VGC27_00690"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3956..3958),aa:Pro,seq:tgg)
     gene            complement(4083..4592)
                     /locus_tag="VGC27_00695"
     CDS             complement(4083..4592)
                     /locus_tag="VGC27_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY0281124.1"
                     /translation="MTALPQAKPAVEWRMRQLFAVLAACALLAACSPRDPQPQTSLPT
                     TKVVIDTQKGPVAFNVEVASDGASLEKGLMYRTKLAEDAGMLFDFRKPTLAVFWMKNT
                     PLSLDILFIRADGVVSTIAADAVPYSEDKIPASEPVRAALEINGGQATAKGIRPGDKV
                     RAAIFGNTR"
     gene            complement(4612..5130)
                     /locus_tag="VGC27_00700"
     CDS             complement(4612..5130)
                     /locus_tag="VGC27_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0281125.1"
                     /translation="MASPSGDAAQEIVGQVKWFDLAKGYGFIKPASGVQGDILLHQTC
                     VRQSGFKAAYEGARVVCEAVQGPKGLQARRLISLDNSTAQPSEVLQQRTARYTAEPRG
                     PAFDATVKWFNRSKGYGFVSRGPDTPDIFVHMETLRRGGIRELRQGQRVRVRAGDGPK
                     GELAAEITVLDA"
     gene            5406..5567
                     /locus_tag="VGC27_00705"
                     /pseudo
     CDS             5406..5567
                     /locus_tag="VGC27_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
     gene            complement(5634..6524)
                     /locus_tag="VGC27_00710"
     CDS             complement(5634..6524)
                     /locus_tag="VGC27_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281126.1"
                     /translation="MKTKLKIALLLGAVTVAAGAELAMAQMDGPQPPDGPMQGLMRSH
                     SRLADRLQHAFDLNHDGKITHDEMNQAIEARFAAATHHAAAMTPDQFAALHRAEFRQH
                     SGEMFRRLDWNGDGGLSADEYAIPQRARFMIMDKEGMGSVSCAPAMRAGHVRKPPQEK
                     ANARRARTQFGSHGNFGLAGFCADNDLNKDGTVTRAEFDAAVGKRFALAAGGAAAMNA
                     AQFAGDLERHFRDGNAKRFKRLDKDGDGKLTLAEFAANELKLFARLDKNKDRAITADE
                     MKSKFRAGGDRNASTKANYH"
     gene            6709..7437
                     /locus_tag="VGC27_00715"
     CDS             6709..7437
                     /locus_tag="VGC27_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0281127.1"
                     /translation="MAKDESKPHILVVEDARDIREPLARYLREHGYRATTAAEASAAR
                     KVMKGSAIDLVVLDIMMPGEDGLSLCRSIRETSQTPVILLTARGEEVDRIVGLEMGAD
                     DYIPKPFSPRELIARIAAVLRRTQALPPRLKPPAAARIRFGDWTLDTGQRELTGHDGV
                     AMPLSSGEFRLLSALLERPKIAVTRNQLLDLTKGRDADLFDRSIDNHVSRLRKKIEPD
                     PKNPRYIKTVWGGGYIFAMDPVTE"
     gene            7434..8792
                     /locus_tag="VGC27_00720"
     CDS             7434..8792
                     /locus_tag="VGC27_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0281128.1"
                     /translation="MKLWPRTLGMQLVIVTSVAVFLSNAAVALWFELGNERFNESALT
                     ERMLDRAASVATLMSSIPPKAREAATRALAGNFWRFQIRSGKTVAQPMTPAEAKLAAR
                     LKAMLPAKLAKMPVTVRFRKVIPAEDMIHGRSRIASEKMAMEMTIPVVRNTQLVATFF
                     RPPAPPWPAEILLAAFVTIVVASAAAAYIARRVARPLSELADAASVVARGGAAPRLRE
                     IGPDDVRNAAIAFNAMTDQVTRTLESQRQLLSAVGHDLRTPITAMRINLEFVEDTELR
                     DRLQRNLDELQVLTEAVLSAARGAGGEPRRQVDLAALVESVCADLDDLGEPVSWAGHK
                     PAPLYCRSNEILRAVRNLIENAVAYGKKAEVRIEEFPDAYEIVVEDEGPGIPEADRSR
                     VFEPFVRLETSRNEDTGGAGLGLTLVKASAEGHGGAVVLEDRPQGGLRVRLRLPREAK
                     RA"
     gene            8822..9877
                     /locus_tag="VGC27_00725"
     CDS             8822..9877
                     /locus_tag="VGC27_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY0281129.1"
                     /translation="MIAQPPRVPRIGAVVLAAGLSSRMGANKLLMPLWGRPLIRHAVE
                     AAVASSADPVIVVTGQHAKEVENSLNGLTVRFVENKEYSKGLSASLKCGLRALPEDCD
                     GAVILLGDMPLVTSSLIDRLIAAFDPVQERAIVVPVRGGRRGIPVLWAKRFFPEMLAL
                     EGDQGAKDLLARHQDLIYEIDAADDGPLIDFDTPEALAAYEAMSDPLYTKAVLRLAAE
                     AKRAGRLPDPHGTYTERNPVCSDRSTVDLIIEGGRIAAMAHDTKACVLAQASASILAA
                     LPGCTYDDLVKLKADVASMLESGKPPAEPFAPYAALSDVAKYPSRQKCVLLPIDAALK
                     AFEASQAREPGSQGSKG"
     gene            complement(9836..11440)
                     /locus_tag="VGC27_00730"
     CDS             complement(9836..11440)
                     /locus_tag="VGC27_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase family protein"
                     /protein_id="HEY0281130.1"
                     /translation="MSTAMPWWRGAVVYQVYIRSFCDGNGDGQGDFAGLIGKLDYIAA
                     LGVDAVWLSPIHPSPNRDWGYDVSDYENVHPDYGTLADFDALLEAAHARGLKIIIDEV
                     LCHTSDEHPWFQDSLNKGAKADWYVWADPKPDGTAPNNWLSAFGGPAWSYQPARRQHY
                     HHKFLRQQPKLNWHNPEAKKAALAVLEFWLARGADGYRLDVANAYLHDPALADNEPVP
                     LAERTPAIWADAAELQQHFHDCNLPENIGCLADIRRTVERHADRFVFGEFSEGYERSG
                     AFAAPDEGLHSAYIFFMLRANKLSPDFVKGNYALLAPHPRHWPCVSFCNHDITRTVTR
                     FGGGADGDPALAKLMLALLFSIKGTVLLYQGEELGLPEVDLRRGQLKDPVGDLYYPLF
                     KGRDGCRTPMPWDAGAPNLGFTTGAPWLPPGKTHRALAVSEQEGDTDSVLAYARRFLS
                     ARRQSTALRLGEMEFLDAPESVLAFARAHDGERMLCVFNMSRDKAAFRHGLISGATAT
                     EFGCGEAKAEGGELSLAPLAAWFARL"
     gene            complement(11437..12036)
                     /locus_tag="VGC27_00735"
     CDS             complement(11437..12036)
                     /locus_tag="VGC27_00735"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY0281131.1"
                     /translation="MSRLDEEEDDKKQNETPEPQPSQVQKALFKSRTVLIFGDIDMKM
                     AERVSAQLLALASEGAGDIRVLINSPGGHVESGDTIHDMIRFVGPRVKMIGTGWVASA
                     GAHIYLGAVKENRFCLPNTRFLLHQPLGGVRGQASDISIEAEEIIKMRERLNRIIARE
                     TGQTFEKVVADTERNFWMGAEEAKAYGLVSKVVARASEV"
     gene            complement(12298..12501)
                     /locus_tag="VGC27_00740"
     CDS             complement(12298..12501)
                     /locus_tag="VGC27_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0281132.1"
                     /translation="MTIGTVKFFNTSKGFGFITPEDGGKDVYVHASAVEAAGLQSLAE
                     GQKVSFDIQPDARGSKAANLKAV"
     gene            complement(12707..12982)
                     /locus_tag="VGC27_00745"
     CDS             complement(12707..12982)
                     /locus_tag="VGC27_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281133.1"
                     /translation="MSNSSDKTDSARRRAQTHFTASEQRDTLVRREIEKERAASNAKT
                     AKLKALRLAKEAVDKEEADRLAAEKALQKTAKTRTRTVPRKKQQPAA"
BASE COUNT         2424 a         4265 c         4192 g         2586 t
ORIGIN      
        1 ggcctcggct tcctgatgct ggtcttcggc tcgaattacc acaatgccgt tttccgggtg
       61 ccgctctatt ggctggtgtt cgcctacttc ctgcattcga tgggcgagct gtgtctgtcg
      121 ccggtggggc tgtcgatgat ctcgaagctg tcggtacaga agatggtggg catgatgttc
      181 ggcgtgtggc tgctctcctc cgcgatggcg cagtatgtcg ccggcatcat cgcccagctc
      241 gcctcgaccg aaaccgtcgc cggcaaggtg ctcaacgcgc aagtgtcgct ggaaacctat
      301 ctgggcgtgt tccagaccat cggcatcgcc gggatcctct cgggctgcgt gctcctggcg
      361 ctgtggccga tcctcaaacg ggggatgcac ggcatccatt gagcatcagc cttgcgtttc
      421 atccccttgt catttcctgc cgcgcaaccg ataaggcggc gggaagcgag atgcaggtga
      481 cgacgtgacg gacgaacgcg acaaacccga aaccatcgcg gtttccgccg gacggatgag
      541 cgaggcgcat ttcggcacgg tcaatacgcc ggtctatcgc acctcgacga tcctgcatcc
      601 cgatgtggcg tcgctgaagt cccacgatgc gccctattcc tatggccggc gcggcacgcc
      661 cacgacgcgc agcttcgagg aagcgatgga agcgctggaa ggcgcggcgc gcaccgtcag
      721 cctgccctcc ggcttgaacg ccatcgccac ggcggtcctg tcggtctgcc gcgcgggcga
      781 tcatctcttg atggtcgata gctgctacgg gccgacgcgt catctctgcg gcaccgtgct
      841 gaagcgcctc ggcgtcgaga ccacgtatta cgatcccttg atcggcgaag gcatcgccgc
      901 gttgctgcgt ccgaacacca gggccgtata ttgcgaaagc ccgggctcgc tcacgtttga
      961 agtccaggac gtgccggcga tcgcggcggc ggcgcacgcc aaaggcgcct cggtgctgat
     1021 ggacaatacc tggggcacgc cgctttactt tccggcgttg gcgcgcggcg tcgacctgtc
     1081 gatccatgcg gcgacgaaat atatctgcgg ccattccgat acgatgatgg gcacggtcag
     1141 cgccaatcaa agccacgccg cgcggctcgg caatttcgtc ggcgatctgg gactttacgc
     1201 cagcggcgac gactgcttcc tggcgctgcg cggattgcgc accctcgccg tgcggctgaa
     1261 gcgccaccag gagaccgcgc tgacgttgag ccgctggctg cttgggcggc ccgaggtttc
     1321 gcgcgtgctc tatccggccc tggaaaacga tcccggccat gccctgtgga agcgggactt
     1381 cctcggcgcc agcggcttgt tcggcgtcgt gctgaagccc gtcggcgaga aatccgttac
     1441 cgccatgatc gacgggctga agcatttcgg catcggctat tcctggggag gctacgagag
     1501 cctgatcatt ccggccgatt tcaaacgcac cgcaaaagca ttcgaggccg aagggccggt
     1561 gatccgcatc catgccggac tggaagacgt agacgatctc cgcgccgatc tcgaagccgg
     1621 tttcgtcagg ttgaacgcct catgaccgat accatttcgc acgaggcctt tctcaacgcc
     1681 gtcaaattca acggcgacgg gcttgtgccg gtcatcgccc aaagccatgc caccggcgcg
     1741 gtgctgatgc tcgcctggat gaaccgccac acgctggagg aaacgctttc caccggacaa
     1801 gtcacctatt ggtcgcgttc gcgcggcaag gtctggcgca aaggcgaaac ctcgggcaac
     1861 acgcagcgcc tggtggaagc ctggatcgat tgcgacggcg atacgctgct tctcaaggtc
     1921 gatcagcttg gcccggcctg ccacaccggc gcgccgtcct gcttcttccg caagcttggc
     1981 taaccgaaca atccacgcaa atgctcgttc aggaattttc ccgccggatc gagcttcgcg
     2041 cgtaacgcgc gaaacgcccc gtactgcgga tagagatccg caagttcgcg cgccgtcagc
     2101 gtatggcgca ttccccagtg cggccgccca tcgtaccggc ggaagaccgc ttcgcacgcc
     2161 tcgaacaacg cggtcgcatc gacccgatga tattgataca cggcgatggt ggcactcttg
     2221 cggcgataga aagggctgag ccagacgtcg tccgccgcga ccgtgcgata ctctatcgga
     2281 aaacctgttt tgatttgccg cttgcgaatc gtctccacga tttcgcgcag aacctccggg
     2341 cccttttcgt aaggcaccgc gtattccatc tcgttgaagc gcacgctgcg cggactgggg
     2401 agtatttcgt ggctccagcg cacatgctgc cttcccggca tcagttggga gaacagacga
     2461 tgcgccggtt tcatcaggag aggcgcgcgt ggcagggcgt cgttgacggc ggtgaagacg
     2521 tcgtcctcga tgcttacgcg ctcgccgcgc gcgcgcaact tttcggcgct gcgcggcgcg
     2581 cgcgcattgg cctcggtttc attgagcgat ttgcagaccg ccgtgtcgga atagggaaac
     2641 cagaagaact tgaaatgacg gttcgcggcg accagcccgt cgagctggcg gaacagttcg
     2701 ccgattgaat gcgagaactc ctttttcacc agcttgtagg tggggcgcac attcatcgcg
     2761 atttcggtga tcacgccgaa tgtgcccagc gaggtgcggc cggcggcgaa aatgtcggcg
     2821 ttctcgttcg cggaacaatc gatcacctcc ccgttcgcct tcaccaggcg aaagccggcg
     2881 acttcggcgg agtagctgcc cagcgcgatc ccgcttccat gcgtgcccgt gctcaccgcg
     2941 ccggccaagg tctggcggtc gatgtcggac atgttcttga ggccgcagcc ggccggacgc
     3001 agcagcgggc cgacttccca caagggcgtc gccgccgcaa tggtcgccac ctggcgttcg
     3061 ggatcgaagc ccaagaggcc gttgaaagcc tttaggtcga tcagcgtgcc gctcgtcgcg
     3121 ttgagcggtg agaaggaatg gccaccgccg ggaacccgca cgacgtcctc ggctttgcgc
     3181 acggccgcgg cgagttcaac ctcgtccctg ggtgcgacaa ccgtgcgcgg acggcaccag
     3241 acgccgcccg accagttcga ccatctgcct ccgacgagtt tcatgcacgc tgcctcagcc
     3301 cggctttgcg ggttgggggt ttgagcagca ccagcgctcc gcgtcaatcc tgcgttcgct
     3361 gagaagatga atcggcccgc atggcaggcg gaaactggcg accccggcgt gactcgaaca
     3421 cgcaacccct tgcttagaag gcaagtgctc tatccggttg agctacgggg tcggtcaggt
     3481 atcagctatt cagtgggacc aaggtacttt tcgatcataa cggaaattgt cggcataggc
     3541 tttaggcttc ggaaccggtc tgtgcggctc gaacaaatcg aaagcgatgc cgtgtctttc
     3601 ggcataggca atcgcctctt ccttgctgtc gaattcgagt tgcacttgcg aaagcatgtc
     3661 ggtggaaccc gtccagccca tcaaggcatc gatccggcgc ggactgacag gttcatgctc
     3721 caacacccag gccttggtgc gggccttgcc cgattgcatc gcggatttgg ccggcatgta
     3781 aattcgcgcg cgcaacggcg tctccctttc gcatcacata cgtatacttt tacagcccgc
     3841 cttcacttcg ttttggcgtg gcatccgcct tcgctatatc acaacgcggt cacgccgtag
     3901 cgtagcgaag gcggatggtc ggggcggcgt gattcgaaca cgcgaccctc tgctcccaaa
     3961 gcagatgcgc taccaggctg cgccacgccc cgtgaaagcc gctcctgtgc tttcgggaca
     4021 aggccgtaca cgaaagcaag gcgtaacttc aacccgtccc gatccatcaa accgccagac
     4081 gcctaccgag tgttgccgaa aatcgccgca cggaccttgt cccccggccg gatgcccttg
     4141 gctgtggcct gcccgccgtt gatttcaagc gcggcgcgaa ccggctcgct ggccgggatc
     4201 ttgtcctcgg aataagggac cgcgtcggcg gcgatcgtcg aaacgacccc gtccgcccgg
     4261 atgaacagta tgtcgagcga aagaggcgtg ttcttcatcc agaagaccgc caaggtcggc
     4321 ttgcggaaat cgaacagcat gccggcatcc tcagccagct tcgtacggta catcagcccc
     4381 ttctccaaag aggcgccatc cgaggcgact tcgacgttga aagccaccgg ccccttttgg
     4441 gtgtcgatca ccacctttgt ggtcggcagc gaggtctggg gctgagggtc gcgaggcgaa
     4501 cacgcagcaa gaagcgcaca ggcggcgagg acggcgaaga gctgacgcat gcgccactct
     4561 accgccggct ttgcctgcgg caaagcggtc attaaaagaa ccggtctgag gtcaggcgtc
     4621 gagcaccgtg atttccgcag ccaactctcc ctttggaccg tcgcccgcgc gtacccgcac
     4681 ccgctggccc tgccgcagtt cccggatgcc gccgcgccgg agcgtctcca tatggacaaa
     4741 aatgtccggc gtgtccggtc cgcgcgaaac aaaaccatag cccttgctgc ggttgaacca
     4801 tttgacggtt gcatcaaagg ccggtccgcg cggctcggcc gtgtagcgtg ccgtgcgctg
     4861 ttgcaacacc tcgctcggct gggccgtcga gttgtcgagg gagatcagtc tacgggcttg
     4921 caggcccttg gggccctgga cggcctcaca gacgacccgc gctccttcat aggcagcttt
     4981 gaagcccgac tggcgaacgc aagtctggtg caggaggata tccccttgca cgccgctcgc
     5041 aggtttgatg aaaccataac ccttcgcgag atcgaaccac ttgacctgac cgacgatctc
     5101 ttgtgcggca tcccccgacg gagaagccat gacgctagta tccatccctt tccccaaaac
     5161 tcatgaaagt aaaaataagg ttaacacagt cgggttccaa ggtgaagtca aagtgtcgcg
     5221 gttttgtcgt aaacagccca tttttggttg ggtgcaggtt gtttgcgttt tttgaggttc
     5281 tttcatggac ttgaccgcca aagccgtgcc cccggcctgg ccaggtaccc ttaggaacgg
     5341 tgtaatatct ggctcccgcc acctccacgg agtggctccc gcatgactga agctcccgac
     5401 gatccagtgc tcgtggcggc gcccgcctca aggtccacgg tgttgccgac gacgcgctaa
     5461 cgcgcatcca cggtcccgtg ggcctcgtga tcggcgccga gagtccggcg gagattgccg
     5521 tctccatcca ggcgcacatg accgaacggc cgcgcacgcg ctcctagtgt cgtggtttcg
     5581 aagttcgcag catgcgcact atcgggctgc aagcgaattt cggaatcggg acactagtga
     5641 taattagcct tcgtgctcgc attgcgatcg cctccggcgc ggaatttcga cttcatctcg
     5701 tccgccgtaa tcgcacggtc cttgttcttg tccaggcgtg cgaacagctt cagttcgtta
     5761 gcggcgaatt cggccagcgt gagcttgccg tcaccgtcct tgtccaggcg cttgaatctc
     5821 ttggcattgc cgtcgcggaa atgccgttcc aggtcgccgg cgaactgggc cgcattcatc
     5881 gccgccgcac cgcccgcagc gagcgcgaaa cgcttgccga ccgcggcatc gaattccgcg
     5941 cgggtcaccg tgccgtcctt gttcagatca ttatcggcgc agaatccggc cagcccgaaa
     6001 ttgccgtgtg aaccgaactg cgtccgcgcg cgccgggcat tggccttctc ctgcggcggt
     6061 ttgcgtacgt gccccgcgcg cattgcgggc gcgcacgaca cgctccccat gccctccttg
     6121 tccatgatca tgaaacgcgc gcgctgcggg atggcgtatt cgtccgccga cagcccgccg
     6181 tcgccattcc agtcgagccg gcggaacatc tcgccggaat gctgccggaa ctcggcccgg
     6241 tggagggcgg cgaactggtc cggcgtcatc gccgccgcat ggtgcgtggc cgccgcgaaa
     6301 cgcgcctcga tggcctggtt catctcgtca tgggtgatct taccgtcatg attgagatcg
     6361 aatgcgtgct ggagacggtc ggccaagcgg ctatggctgc gcatcagccc ttgcatcggc
     6421 ccgtccggcg gctgcggccc gtccatctgc gccatggcaa gctcagcacc ggcggcgacg
     6481 gtcactgccc ctaaaagcaa ggcgattttc agtttcgttt tcaagataga ctcccctcaa
     6541 atcgacaccc gctaaatcaa tccgaggttg tggtagaact ttgccggaac aaaaattaat
     6601 tttcgcaaag ttcctgcggg ttacagcgtt cgacaaactt tgcggcaaat gggggtttgc
     6661 ggtgtcaaca cggacgccgc acagtggggc atgctagaat tccctgccat ggccaaggac
     6721 gaatcgaagc cgcatatcct tgtggtcgag gatgccagag acattaggga gccgctcgcc
     6781 agatacctgc gcgaacacgg ttatcgtgcg acgaccgctg cggaggcgtc ggccgcgcgc
     6841 aaagtcatga agggctcggc catcgatctg gtcgttctcg atatcatgat gcccggcgaa
     6901 gacggactga gcctttgccg ctcgatccgc gagacctcgc agacccccgt gatcctgctg
     6961 acggcgcgcg gcgaggaagt cgaccgcatc gtcggcctgg aaatgggcgc cgacgactat
     7021 attccgaagc ccttcagccc gcgcgaactg attgcacgta tcgccgccgt tctgcgccgc
     7081 acgcaggcgc tgccgccgcg cctgaaaccg ccggccgccg cgcgcatccg cttcggcgat
     7141 tggactctgg acaccggcca acgcgagttg acggggcacg acggcgtggc gatgccgctg
     7201 tccagcggcg agttccggct tttgagcgcg ctgcttgagc ggcccaagat cgccgtgacg
     7261 cgcaatcagc tgctcgacct caccaaaggg cgcgacgccg atcttttcga ccgctcgatc
     7321 gacaatcatg tcagccgcct gcgcaagaag atcgagcccg atcccaagaa cccccgctac
     7381 atcaagacgg tttggggcgg cggctacatt tttgccatgg acccggtgac ggaatgaagc
     7441 tgtggccgcg tacgctgggt atgcagctcg ttatcgtcac ctccgtggcg gtgtttctct
     7501 ccaacgccgc cgtagcgctg tggttcgagt tgggcaacga gcggttcaac gaaagcgcgc
     7561 ttaccgaacg gatgctggac cgcgcagcct ccgtcgccac gctgatgagt tccattcctc
     7621 cgaaggcgcg cgaggcggcg acgcgtgcgc tcgccggcaa tttttggcgg ttccagattc
     7681 gctccggcaa gaccgtcgcc cagccgatga ccccggcgga ggcgaagctg gccgccaggc
     7741 tgaaggcgat gcttccggcc aaattggcga aaatgcccgt gaccgtccgc tttcgcaaag
     7801 tgattccggc cgaagacatg atccatggac gcagccgcat cgcttcggaa aaaatggcga
     7861 tggaaatgac gataccggtg gtgcgcaaca cgcagctggt ggcgaccttc ttccgccccc
     7921 cggcgccgcc ctggccggcc gagatcctgc tggccgcctt cgtcaccatt gtcgtcgcct
     7981 cggccgccgc cgcctatatc gcgcggcggg tggcgcggcc tctgtccgaa ctggcggacg
     8041 cggcgtcggt ggtggcgcgc ggcggcgcgg ccccgcgcct gcgggagatc ggacccgatg
     8101 acgtccgcaa cgccgccatc gccttcaacg ccatgacgga ccaggtgacg cgcaccctcg
     8161 aaagccagcg ccagcttctg tcggcggtgg gacacgatct gcgcaccccg atcaccgcga
     8221 tgcggatcaa cctcgaattc gtcgaagaca cggaactgcg cgaccggctt cagaggaatc
     8281 tcgacgagct gcaagtcttg accgaagcgg tgctgtcggc ggcgcgcggc gcggggggcg
     8341 aaccccgccg ccaggtcgat ctggcggctt tggtcgaaag cgtctgcgcc gacctcgacg
     8401 atctgggcga gccggtaagc tgggcggggc acaagccggc gcccttgtac tgccgttcga
     8461 acgagatcct ccgcgccgtc cgcaacctga tcgagaacgc ggtggcctat ggcaaaaagg
     8521 ccgaggtgag gatcgaggag ttccccgacg cctacgagat cgtggtcgag gacgaaggcc
     8581 ccggcattcc cgaagcggac cgcagccggg tgttcgaacc cttcgtccgg ctcgaaacct
     8641 cgcgcaacga ggatacaggc ggggcgggcc tcggacttac gctggtcaag gccagcgccg
     8701 aaggtcatgg cggggcggtc gttctggaag atcgccccca aggcggcctg cgcgtgcgct
     8761 tgcgtttgcc gcgagaggca aaacgcgctt aagaactccc ggaatcgtgt ggagaaccag
     8821 tgtgatcgca cagccgcctc gcgtgcccag gatcggcgcc gtcgttctgg cggccggtct
     8881 gtcgtcgcgg atgggcgcca acaagctgct catgccgctt tggggtaggc cgctcatccg
     8941 ccatgccgtc gaggcggcgg tggccagcag tgccgatccc gtcattgtcg tcaccggaca
     9001 gcatgccaag gaggtggaaa actcactgaa tgggctgacc gtccgtttcg tggaaaataa
     9061 agagtattca aagggtttaa gtgcatccct caagtgcggt ctgagggcat tgccggagga
     9121 ttgcgacggc gccgtgatcc tgctcggcga catgcctctg gtgacttcaa gtctgatcga
     9181 ccgtctgatt gcggccttcg atccggtgca ggaacgcgcc atcgtggtgc cggtccgtgg
     9241 cggccgccgg ggcattcccg tgctctgggc caagcgattc ttccccgaga tgctcgctct
     9301 cgagggtgac cagggcgcga aggatctgct ggcccggcac caggacctga tctacgagat
     9361 cgacgctgcc gacgacggcc ccttgatcga cttcgacacg cctgaagcgc tggcggcgta
     9421 cgaggccatg agcgatccgc tctacaccaa ggccgtgctt cggctggccg ccgaggcgaa
     9481 acgcgcgggc cgtctgcccg atccgcacgg tacgtacacc gagcgcaatc ctgtatgcag
     9541 cgaccgttcg accgtcgatt tgatcattga aggcggccgc atcgcggcca tggcccacga
     9601 taccaaagcg tgcgtgctgg cgcaggcttc ggcctcgatc ctagctgccc tgcccggctg
     9661 cacctatgac gacctggtga agctcaaggc ggatgtcgct tccatgctcg aaagcggtaa
     9721 gccgccggcg gagccgttcg cgccttatgc cgccttgagc gacgtcgcca aatatcccag
     9781 ccggcagaaa tgcgtgctgc tgcccatcga cgccgcgctc aaggccttcg aggcgtcaca
     9841 ggcgcgcgaa ccaggcagcc aagggagcaa gggatagttc gccgccttcc gctttcgcct
     9901 cgccgcatcc aaattccgtc gccgtcgcgc ccgatatcaa gccgtgacgg aatgcggctt
     9961 tgtcgcggct catattgaag acacatagca tccgttcgcc atcgtgcgcg cgcgcgaagg
    10021 ccaggacgga ttcgggtgca tccaggaatt ccatctcgcc taggcgcaac gccgtgctct
    10081 gtctgcgcgc cgacaagaac cgtctggcat aagcgaggac ggaatccgta tcgccctcct
    10141 gctctgacac ggccagagcg cggtgcgttt taccgggcgg aagccacggc gcaccggtgg
    10201 tgaaccccag attcggcgcg cctgcatccc agggcatcgg cgtgcggcat ccgtcgcgcc
    10261 ccttgaacag cgggtaatag aggtcgccga ccggatcctt gagctggccg cggcgaagat
    10321 cgacttccgg caggcccagt tcctcgccct gatagagcag caccgtgcct ttgatggaaa
    10381 acagcagcgc cagcatcagt ttggccaggg ccggatcgcc atccgctccg ccgccgaagc
    10441 gtgtcacggt gcgtgtaatg tcgtggttgc aaaaagagac gcagggccag tggcgcggat
    10501 ggggcgccag cagcgcgtaa ttgcccttga cgaaatccgg cgacagcttg ttcgcccgca
    10561 gcataaaaaa gatgtaggcg gagtgcagcc cttcgtccgg tgcggcgaag gcgccggagc
    10621 gctcatagcc ttcggagaac tcaccgaaga cgaaccggtc ggcatggcgt tccaccgtgc
    10681 ggcggatgtc ggccagacag ccgatatttt cgggaagatt gcagtcgtga aaatgctgct
    10741 gcaattccgc cgcgtccgcc cagatcgcgg gcgtgcgttc ggcgagcggg acgggttcat
    10801 tgtcggcaag cgccggatcg tggagatagg cgttggcgac gtccaggcgg tagccgtcgg
    10861 ccccgcgcgc cagccagaac tccagcacgg ccagcgcggc cttcttcgct tcgggattgt
    10921 gccagttcag cttgggctgc tggcgcagga atttgtggtg ataatgctgg cggcgcgccg
    10981 gctgatagga ccaagccggt ccgccgaacg ccgacaacca attgttcggc gcggtgccgt
    11041 ccggcttggg atcggcccag acgtaccaat cggcttttgc gcctttgttc agactgtcct
    11101 ggaaccaagg atgctcgtcc gaggtgtggc acagcacttc gtcgatgatg atcttcagcc
    11161 cgcgcgcatg agccgcttcg agcagcgcat cgaaatcggc cagggtgccg taatcgggat
    11221 ggacgttctc gtaatcggag acgtcatagc cccaatcgcg gttgggcgaa ggatggatcg
    11281 gcgacagcca gacggcatcg acgccgagcg cggcgatgta atcgagcttc ccgatcaggc
    11341 cggcgaaatc gccctgcccg tcgccattgc cgtcgcagaa gctgcggata tagacttgat
    11401 aaacgaccgc cccgcgccac cagggcatgg ccgtgctcac acctcgctcg cgcgggcgac
    11461 gaccttggag accagcccgt aggctttggc ctcctccgcg cccatccaga aattgcgctc
    11521 ggtatcggcg acgacttttt cgaaggtctg cccggtctcg cgcgcgatga tgcggttcag
    11581 ccgttcgcgc atcttgatga tttcctcggc ctcgatggag atatcggagg cttggccgcg
    11641 cacgccgccg agaggctggt gcagcaagaa gcgggtgttg ggcaggcaga agcggttttc
    11701 cttgaccgcg cccaaataga tgtgcgcccc ggcgctggcc acccagccgg tgccgatcat
    11761 cttcacccgc gggccgacga agcggatcat gtcgtggatc gtgtcgccgg attccacatg
    11821 gccgcccggc gaattgatga gaacgcggat atcgccggcg ccctcggagg caagcgccag
    11881 aagctgggca ctgacccgct cggccatctt catgtcgata tcgccgaaga tcagcacggt
    11941 gcgcgacttg aacagcgcct tctgcacttg tgagggctgc ggttcgggcg tttcgttctg
    12001 cttcttgtcg tcttcttctt cgtcgaggcg ggacatgagt gctcctgtga caagggacga
    12061 acctgggaaa tactagggtg cgggtgtcac ggccagagga aaatgcacgc accgagccaa
    12121 gcttggcgtc catgtgctta aagcatgtta acggaagggg ccggactgga ttaccgtcct
    12181 ggccctctat ggacgatcag acgcgcgccg agcaggacat atccccgccg gggacattcc
    12241 cacagcagac tggcgcaagc cgatccgccg cctagccgcc cgatcgcgaa attcgagtca
    12301 aacggccttc agattggccg ccttcgagcc gcgcgcgtcc ggctggatgt cgaacgacac
    12361 tttttgccct tcggccaggc tctgcaaccc cgccgcttcg acggccgagg catggacgta
    12421 aacgtccttg ccgccgtctt ccggcgtaat gaatccaaag ccctttgaag tgttgaagaa
    12481 cttcactgtt ccgatggtca tcgattggtc cttagtcatc gggttccgca tgagcaaaaa
    12541 tactccgcgg tcgcgagagg cgctgagatc aggagaaggt ccgactgttc gtccgggcgc
    12601 gcagcattgc gggcatggat tcaaacaaca gatgcgctca tctgacggga cgccgtcgcg
    12661 tgcctctgta tagccgtcta tttaccaaag cacaatggat tgcgggtcag gcggccggct
    12721 gctgtttctt gcggggcact gttctggttc tggtttttgc ggttttttgc agtgctttct
    12781 ctgccgcaag tctgtccgcc tcttccttgt cgacggcttc cttcgcaagg cgcaacgcct
    12841 tcagctttgc ggtttttgca ttgctggccg cacgttcctt ttcgatctcc cggcgcacca
    12901 gcgtgtcgcg ctgttcagat gcggtgaaat gcgtctgcgc acggcggcgg gcggaatcag
    12961 ttttgtcgct ggaattggac atgtggttct ttcatcaatt gtctcggcgc ccgggcggtt
    13021 atcgccgacc ggggccattc ctctgcacga acgagagagg cgcaacgctg cgcctgggaa
    13081 ttgataccac ccaatgtcat caaaaggaat ttttccagac gaatactttg cggccggctt
    13141 tcgcttgcgg ccgccgcggc gcggtcctgg cgcctgcgcc ttgctgttaa taccgtaatt
    13201 acagaggctt ggacgccacg cagaaggaat tgtgtcgcgt ctggtgcatc ccttccggga
    13261 acaagaaaat cgtgacgttt gctaggtgac gaccctctcg cctcgccaac gctttaactc
    13321 gacgggctgc cgccaagaaa tcgagccgtt tgccaagatt taagcggcca gaggtgctct
    13381 gctttcatta cggcgggccg agatgtcctg gtgcagattg gttgataact tgacttgtgg
    13441 ataaatgttg catattaacc atctgtt
//
LOCUS       DASWPU010000035         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_67607,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000035 DASWPU010000000
VERSION     DASWPU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67607"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VGC27_00750"
     CDS             complement(<1..437)
                     /locus_tag="VGC27_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281134.1"
                     /translation="MARKFAACSLFALAASLTAAAAADLDASKAKKLPVSDGSAGSFD
                     AHFGDGNGLVWFNVDTGIAWDAASGGYVAFTLPFKVVTWNGQTFNSYNFTDMTVEPTV
                     KLVIYGTNPAMFLAKGDISFAGQFTIAAAAGAGGAAPDPYNPP"
     gene            511..1893
                     /locus_tag="VGC27_00755"
     CDS             511..1893
                     /locus_tag="VGC27_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281135.1"
                     /translation="MAATTGNTGQMRAFQDMAAEIGQKPTSRRFDTWKEIASYFGRDE
                     RTVRRWETGRGLPIHRVPGGAGGTVYAFEAELAAWLRGNPENIEPKEPVPTKTTPPAS
                     RFMLLWPILAILSVTVAFGIWFTSDHGAETPLATSHDPQAAASYRLGLYEWQTRTPVG
                     LANAVKDFKRAIARDPNFAQAYAGLANSYNLLREYTTMPPGTAYPLAKVAAEKAIALD
                     PKIGEAHAALAFDDFYWSRDVRGARREFLSALALEPSSAAIHHWYATFLMTNRAFAQA
                     LVEIEKAEALESSSSAILADKGLILFYDGKGDEAVALLRRLEQAQPDFLSPHQYLSSI
                     YRAQGDDKHFLAEIGEAARAQHSETGKAVADAGMAGIAAGGRAKMFSAMLDVEKRLYA
                     QGKISAYELAVACADAGDEPNAIAYLRVSLSRRETENVALAVDPAFEDLRAKPEFQPL
                     LSMAGLASHA"
     gene            complement(1900..>2933)
                     /locus_tag="VGC27_00760"
     CDS             complement(1900..>2933)
                     /locus_tag="VGC27_00760"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY0281136.1"
                     /translation="KSLQPKAVHDAGVWRLPDGPAYYAASLEASTTTKMKPDEIHQLG
                     LDVVKDHTAKIDAIMQANGLTKGTVGERLRQMYNDPKFRYENSDAGKDKLLADLNVKV
                     QTIRAKLPGYFGALPKADLIIKRVPKYIEAGAPGGYYNNGSLDGTRPGTYYINLRDTA
                     EVPSWTLPTLTYHEGIPGHHLQISISQESPLPLIRKVGGFTAYIEGWALYAEQLAGEM
                     GMYEGDPWGQIGQLHDSMFRGVRLVVDSGMHAKKWSREQAVKYYVDTLGDQEASAITE
                     IERYCVWPGQACSYMVGKLTILRLRDKAKAALGAKFDIHKFHDTVLLSGALPLDVLEK
                     VIDDYIAKA"
BASE COUNT          546 a          889 c          936 g          562 t
ORIGIN      
        1 taaggggggt tgtatggatc gggcgcggcg ccgcccgcgc cggcagccgc ggcaatggtg
       61 aattgacccg cgaaggagat gtcccctttg gcgaggaaca tcgctggatt ggttccgtag
      121 atcaccagct tgaccgtcgg ctccacggtc atgtcggtga agttgtagga attgaacgtc
      181 tgcccgttcc acgtcaccac cttgaacgga agggtgaacg cgacataccc gccgctggcg
      241 gcgtcccagg cgataccggt atcgacgttg aaccagacca ggccgttgcc gtcgccgaaa
      301 tgcgcgtcga agctacccgc gctgccgtcg ctgacgggca gcttcttggc tttcgaggcg
      361 tcgagatcgg ctgcggccgc cgcggtcaac gacgcggcaa gcgcgaacaa cgagcaggct
      421 gcaaatttgc gggccatgag agactccaga catcgccggt tgcggcgatt gcggttggca
      481 ggaagaccgc gcaaacttga tggacccgtc gtggcagcca caacgggtaa taccgggcag
      541 atgcgggcat ttcaggacat ggcggcggaa atcggccaaa aacccacctc gcggcgcttc
      601 gatacctgga aagagatcgc cagctatttc gggcgtgatg aacgcaccgt tcgccgttgg
      661 gaaacaggcc gtgggcttcc catccaccgc gttcccggcg gcgccggggg aacggtctac
      721 gcgttcgaag cggagcttgc ggcgtggctg cgcggcaatc ccgaaaatat tgagcccaaa
      781 gagccggtcc cgacaaagac gacgccacct gcgagccgct tcatgctgct ttggccaata
      841 ctcgcgatcc tgtccgtcac cgtggcattt ggtatctggt tcacctccga tcacggggcc
      901 gagacaccgc tggcgacgag ccacgacccg caggccgccg cgtcataccg gttgggcctt
      961 tatgaatggc agacgcgcac gccggtggga ttggcgaacg ccgtgaagga tttcaaacgg
     1021 gcgatcgcgc gcgaccccaa tttcgcgcag gcctatgccg ggcttgccaa cagctacaac
     1081 ctgctgcgcg aatataccac gatgccaccg ggcacggcgt atccgctcgc caaggtggct
     1141 gccgaaaagg cgatcgcgct cgatccgaag atcggtgagg cccatgccgc gctggcattc
     1201 gacgatttct actggtcgcg cgatgtgcgc ggtgcaaggc gggaatttct gtcggcgctt
     1261 gcgctggagc cgagtagtgc cgcgatccat cactggtacg caacgtttct gatgaccaat
     1321 cgggcctttg cgcaagcgct tgtggaaatc gaaaaagcgg aagcgctgga atcgtcgtcg
     1381 agcgcaatcc tcgccgataa aggcctgatc ctgttttacg acggcaaggg tgacgaggcg
     1441 gtcgcgttgc tgcggcggct cgagcaggcg caaccggatt tcctatcgcc gcatcaatat
     1501 ctgtcgtcca tctatcgcgc gcagggcgac gacaagcatt tcctagcgga aatcggcgaa
     1561 gccgcgcgcg cgcagcacag cgaaaccggc aaagcggttg cggacgcagg catggcggga
     1621 atcgcagcag gcggccgtgc gaagatgttc agcgccatgt tggacgtcga gaaacgtctc
     1681 tacgcccagg gaaaaatatc ggcttatgaa ttggcggttg cctgcgccga cgccggcgat
     1741 gagcccaatg ccatcgcata tttgcgggtg agcctgtcac gccgcgaaac ggaaaacgtc
     1801 gcgcttgccg tcgatcctgc atttgaagac ctgcgtgcga agcccgagtt ccagccactc
     1861 ttatcgatgg ccggtctggc ttcgcacgcc tgaatccgct caggccttgg cgatatagtc
     1921 gtcgatgact ttctccagca cgtccagcgg caaggcgccc gagagcagca ccgtgtcgtg
     1981 gaatttgtgg atgtcgaatt tcgcgcctag tgccgccttc gccttgtcgc gcagacgcag
     2041 gatcgtgagc ttgcccacca tgtaggagca ggcctgcccc ggccatacgc aatagcgctc
     2101 gatctcggtg atggctgagg cttcctggtc gcccagcgtg tcgacatagt atttcaccgc
     2161 ctgctcgcgg ctccatttct tggcatgcat cccgctatcg acgacaaggc gcacgccgcg
     2221 gaacatggaa tcgtgaagct ggccgatctg gccccacgga tcgccttcgt acatgcccat
     2281 ctcgccggcg agctgctcgg cataaagcgc ccagccttcg atataggcgg tgaacccgcc
     2341 aaccttgcgg atgagcggca agggcgattc ctggctgatc gagatctgca ggtgatggcc
     2401 cggaatgcct tcgtgatagg tgagcgtggg caaggtccag ctcggcactt cggcggtgtc
     2461 gcgcagattg atgtagtagg tgcctggacg cgttccgtcg agcgagccgt tgttgtaata
     2521 gccgcccggc gcgccggcct cgatgtattt cggcacgcgc ttgatgatga gatcggcctt
     2581 cggcagcgcg ccgaaataac cgggcagctt ggcgcggatc gtctgaacct tcacgttcag
     2641 atcggcgagc agcttgtcct tgcccgcatc ggagttctcg tagcggaatt tcggatcgtt
     2701 atacatctgc cgcaggcgtt cgccgaccgt gcccttggtc agcccgttcg cctgcatgat
     2761 ggcgtcgatc ttcgcggtgt gatccttcac cacgtcgaga cccagctggt ggatctcgtc
     2821 gggcttcatc ttggtcgtgg tcgaggcttc gagcgaggct gcgtagtacg ctggcccgtc
     2881 gggcaggcgc catacaccgg cgtcatgcac ggccttcggc tgcaagctct tga
//
LOCUS       DASWPU010000036         4370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_110332,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000036 DASWPU010000000
VERSION     DASWPU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110332"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1673
                     /locus_tag="VGC27_00765"
     CDS             177..1673
                     /locus_tag="VGC27_00765"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY0281137.1"
                     /translation="MQGKSALMAGAAAVALVMSAQAGLAGKGASEGGSNSSSSQATSG
                     PSNAELAARIQALEDQLQASELKVDNDHSRLSSLEQNFNDTVWTFSNGRPIVTSGDKR
                     FSMIFRVRFQSDFGAFMQDDPTAAGNQAQYKDLGTGMVVRRAHLGVQGIAFKDFWYEF
                     RYNMGGSDAEAAGLSLARVAYTGIPNFRINVGVIEPAFMHEGTTSSSQLMFMERPEID
                     NIAADVFGAGDARRGIELVWQKAGLFKPDDNLVLMTALTGSATGSATGHANGGDEQSQ
                     ILGRLSYRFWNDGASNAMFGVSAGHVLNSAGQGIRFRDRPQIRINGDRLIDANFAAAK
                     RATMYAFDGGININNFFLGGEYANFMPDVPGTSDDPEFHGWYVEGSWILTGETKGYTV
                     SALNNEIGGWTGPKVANPFSLAGDSWGAWEVVARYSDTNLDWHRLSASGTRGGKERII
                     NLGINWYLNNQVRLGLYDQIVKVDKLNASYAQIGQDINVIGARLQFTN"
     gene            1718..2758
                     /gene="pstS"
                     /locus_tag="VGC27_00770"
     CDS             1718..2758
                     /gene="pstS"
                     /locus_tag="VGC27_00770"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY0281138.1"
                     /translation="MNLSFKSLMTAAAGIVSAAVIASSAFAATVSGAGASFPYPIYAK
                     WSGAYQSIAGFQLNYQSIGSGGGIAQIKARTVAFGATDAPLKPAELDKFGLAQFPTVI
                     GGVVPVVNVPGLAPGQLVLDGQTLANIFLGKITNWNDPAIKALNPGVNLPNKAISVVH
                     RSDGSGTTFIFTTYLSRVSNEWKTNVGASTAVDWPLGIGAKGNEGVAGNVAQSSGSIG
                     YVEFAYAKVNHLKHTRMINRDGKTVSPTADTFKAAAANADWVGAANQGFYIILVDQPG
                     ATSWPITATTYILVYKKPADVSGTAETLKFFKWAYASGGDAALSLDYVPLPPNAVQAI
                     QASWKDIQVPVN"
     gene            2880..3908
                     /gene="pstS"
                     /locus_tag="VGC27_00775"
     CDS             2880..3908
                     /gene="pstS"
                     /locus_tag="VGC27_00775"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY0281139.1"
                     /translation="MNGLKTFAAAAAMAAMAASGALAASISGAGATFPYPVYAKWAGA
                     YQKVSGVGLNYQSIGSGGGIAQIKAKTVVFGATDMPLKPDVLAPLGLVQFPTVIGGIV
                     PVVNIQGVAPGQLVLDGPTLAGIFLGQIAKWNDPRIAKINPGVKLPSQPIVVVHRSDG
                     SGTTFLFSTYLARASKDWRNNVGVNTSVSWPLGIGAKGNEGVAACVAQFGGAIGYVEY
                     AFAKQNHLKFTRLINKDGKPVAPTAETFGAAAANMDWGAASKQNFYALLIDQPGAASW
                     PITATTYILVYKRPVDPKSSAEVLKFFKWAFASGDGMALGLGYVPLPDNAARVIEASW
                     KQIQGSGM"
     gene            4117..>4370
                     /locus_tag="VGC27_00780"
     CDS             4117..>4370
                     /locus_tag="VGC27_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0281140.1"
                     /translation="MSTQLFDNDLLSTLHDVARLMRTRFDRFARTFGMTRAQGLILLR
                     LQHHPGLSQNELAVTCDVEPITVARLIDRLEAGGYVKRCA"
BASE COUNT          868 a         1377 c         1306 g          819 t
ORIGIN      
        1 attcacaacc gtctgttgct gtcatattta cttcatcccc ctgtcacatc gctgtcgccg
       61 cccactggaa tccatccctg cgggcaaaaa ggaccgctgg ccgacgggtg ctacttggga
      121 tgcacattgc ggcttcgata tcgggtcttt tcccttaacg actaagagag ggttcaatgc
      181 aggggaaatc agctttgatg gccggggccg ccgcggtggc gctggtcatg tcggcgcaag
      241 ccggcctagc cggcaagggc gcgtccgagg gtgggtccaa ttccagttcg tcgcaggcga
      301 catcggggcc gagcaacgcg gaactcgccg cgcgcataca ggcgcttgag gaccagcttc
      361 aggcgagtga attgaaagtg gacaacgatc actcccgttt atcgtctctg gagcagaact
      421 tcaacgacac ggtgtggacg ttcagcaacg gccgcccgat agtgacctcg ggcgacaagc
      481 ggttcagtat gatattccgc gtgcgtttcc agtcggactt cggcgcattc atgcaggacg
      541 atccgaccgc cgccggcaac caagcccagt acaaggacct tgggacaggc atggtcgtgc
      601 gccgcgccca tctcggcgtc cagggcatcg cgttcaaaga tttctggtat gaattccgtt
      661 acaacatggg tggttcggat gcggaagccg cgggcctctc gctggcgcgc gttgcctata
      721 ccggcattcc caactttcgc atcaacgtcg gcgtcatcga acccgcgttc atgcatgaag
      781 gcacaacctc atcttctcag ttgatgttca tggagcggcc ggaaatcgac aatatcgccg
      841 ccgacgtgtt tggcgcgggc gatgcccgcc gcggcatcga actcgtctgg cagaaggcgg
      901 gcctgttcaa gcccgacgat aatctggtgc tgatgacggc ccttaccggc agcgccaccg
      961 gttcggccac cggccacgcc aatggcggcg acgagcagtc ccagatcctg ggccgcttgt
     1021 cctatcgttt ctggaacgac ggggcctcca acgccatgtt cggcgtcagt gccggccacg
     1081 tattgaactc cgccggccag ggcatccgct tccgcgaccg tccgcaaatc cgcatcaacg
     1141 gcgaccgcct gatcgatgcg aacttcgcgg ccgccaagcg cgccacgatg tatgccttcg
     1201 acggcggcat caacatcaac aacttcttcc tgggcggcga atacgccaac ttcatgccgg
     1261 acgtgcccgg cacgtcggac gatccggaat tccatggctg gtatgtcgaa gggtcgtgga
     1321 tcttgaccgg tgaaaccaag ggctacaccg tcagcgcact gaacaacgaa atcggcggct
     1381 ggactggtcc gaaggtcgcc aatccgttct cgctggccgg cgattcctgg ggcgcctggg
     1441 aagtcgtggc ccgctacagc gacaccaatc tcgactggca caggttgtcg gcgagcggca
     1501 cgcgcggcgg caaagagcgg atcatcaacc tcggcatcaa ctggtatctc aacaaccagg
     1561 tacgcctcgg cctctatgac cagatcgtca aggtcgacaa gctgaacgcg tcctacgccc
     1621 agattggcca ggacataaac gtcataggcg cacgcctgca attcaccaac tagccaatgt
     1681 ggatagagat caactctaac catcgagagg aagagaaatg aatttgtcct tcaaatcgct
     1741 gatgaccgcc gctgcgggga tcgtatccgc ggccgtgatc gcaagcagcg cgttcgccgc
     1801 taccgtttcc ggcgccggcg cgtcgtttcc gtacccgatc tatgccaaat ggtcgggagc
     1861 ctatcagagc atcgccgggt tccagctcaa ctaccagtcc atcggttcgg gcggcggcat
     1921 cgcgcagatc aaggcgcgga cggtcgcttt cggagccacc gatgcgccgc tcaagccggc
     1981 cgaactggac aagttcggac tggcgcaatt cccgaccgtc atcggcggcg tcgtgccggt
     2041 ggtgaacgtt cccggcctcg ctccgggcca actggtcctc gacggccaaa cgctcgccaa
     2101 catctttctc ggcaagatca cgaactggaa cgatccggcc atcaaggcgc tcaatccggg
     2161 cgtcaatctt cccaacaagg cgatctccgt cgttcatcgc tcggacggat cgggcacgac
     2221 tttcatcttc acgacctatc tgtcgcgcgt cagcaacgaa tggaagacca acgtaggcgc
     2281 aagcaccgcg gtcgattggc cgctcggcat cggcgccaag ggcaatgagg gcgttgcggg
     2341 caatgttgct cagtcgtccg gctcgatcgg ctatgtcgag ttcgcctacg ccaaagtaaa
     2401 ccacctcaag catacccgca tgatcaacag ggacggcaag accgtcagcc cgacggcgga
     2461 taccttcaaa gccgccgcgg caaatgcgga ttgggtcggc gcggcgaacc agggcttcta
     2521 catcatcctc gtcgatcagc cgggcgccac gtcctggccg atcacggcaa cgacctacat
     2581 cctggtctac aagaagccgg ccgatgtcag cggcacggcg gagaccctca aattcttcaa
     2641 gtgggcctat gccagcggcg gcgacgctgc gttgagcctc gactatgtgc ctctgccgcc
     2701 gaacgcggtg caggcgatcc aggcaagctg gaaggatatc caggttcctg taaattaatc
     2761 cggcgtgaat tgcctgccgt aacggggcag gccatacggg acaggggcgg tcgccagacc
     2821 gcccctgttt ttttgcctgt gatactgtgc cggcaccgga ctcaggttgg aggtgcttag
     2881 tgaacgggtt gaaaactttc gccgccgcgg ccgccatggc ggccatggcc gcctcaggcg
     2941 ccttggcggc cagcatctcg ggcgccgggg caacttttcc ctatccggtc tacgccaaat
     3001 gggcgggggc gtatcagaag gtcagcggcg tgggcctcaa ctaccaatcc atcggttcgg
     3061 gcggcggcat cgcgcagatc aaggccaaga cggtggtttt cggggcgacc gacatgccgc
     3121 tcaaacccga cgtgctggcg ccgctcggac tggtccagtt cccaaccgtc attggcggca
     3181 tcgtgccggt ggtgaacatc caaggcgtcg cgccgggcca gttggttctc gacggcccga
     3241 cgcttgccgg aatcttcctc ggccagatcg cgaaatggaa cgatccgcgg atcgccaaga
     3301 tcaatcccgg cgtgaagctg ccaagtcagc ccatcgtcgt cgtccatcgt tccgacggct
     3361 ccggcacgac cttccttttc tcgacctatc tggcgcgcgc cagcaaggat tggcggaaca
     3421 atgtcggggt gaacacgtcg gtgagttggc cgctcggcat cggcgccaaa ggcaacgaag
     3481 gcgtcgcggc ctgcgtcgcc cagttcggcg gggcgatcgg ctatgtcgaa tacgcctttg
     3541 ccaaacagaa ccatctcaaa ttcacccgct tgatcaacaa ggacggcaag cccgtcgcgc
     3601 ccacggcgga gacgttcggg gccgccgcgg caaatatgga ttggggcgcg gcgagcaagc
     3661 agaatttcta cgcgctcctc attgaccagc ccggcgccgc gtcttggccg atcacagcca
     3721 cgacctacat cctggtctac aagcggccgg tcgatcccaa atcgtccgcc gaggtcctga
     3781 agttcttcaa atgggccttc gccagcggcg acggtatggc tctgggcctc ggatacgtgc
     3841 ccctgccgga caatgccgcg cgggtgatcg aagcgagctg gaagcagatt caaggctcgg
     3901 ggatgtaggt ccggccgctc caggcggaca aacagagcaa ggtggccggc cggtcacccc
     3961 agttttttgc tttgcgaccg caagtcgctg gagctttgcc caaaaatcgc ccggatcatg
     4021 gttatatacc atatatcata acgccggtta taatattgtg cgttattata agcatagcta
     4081 tattatagca gcttatgaac cacaaaacag gccaagatgt cgacgcaact gtttgataac
     4141 gatctgcttt ctaccctgca tgacgtcgcc cgcctgatgc ggacgcgctt cgaccgtttt
     4201 gcccgtacct tcggcatgac gcgcgcccaa ggactcattc tgctgcgtct gcagcaccat
     4261 ccgggcctgt cgcagaacga actggcagtc acttgcgacg tcgaaccgat caccgtggcg
     4321 cggctgatcg accggctgga ggcgggcggc tacgtcaagc gctgcgccga
//
LOCUS       DASWPU010000037         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_104227,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000037 DASWPU010000000
VERSION     DASWPU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104227"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VGC27_00785"
     CDS             <1..1126
                     /locus_tag="VGC27_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281141.1"
                     /translation="FDQWPLAQNLSFTFSDRFSFTENDGIVFPRDAVRNDLREAYFTW
                     EPTTATFLEFGRVNLKNGIALGFNPTDFFKTRSAVALASADPSALRGDRLGTLMLRAQ
                     TIWDGGSATFVYAPKLHTPRPLVGAAPSWIDPGFDQTNASDRFLFALNLELGDLSPQA
                     LVYHESGRTKIGLNISRPIGQSVIAYGEWTGGSEPNLIAQAIAYGKATGTLPSFAPVL
                     PPASTSHAFQNDLAVGASWTSEAKITLNLEYHFHQAGFSGADWNNWFDIGAAGPLAAS
                     ELWYVRGFASAMQQPISRQQIFWRADWQDAFIQNFELSGISFVNLTDGSGMAQISANY
                     NLSNAWSVGLYAGGTFGGRRSEWGSLPGASNAIFQVVRYF"
     gene            1450..2061
                     /locus_tag="VGC27_00790"
     CDS             1450..2061
                     /locus_tag="VGC27_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281142.1"
                     /translation="MKIKLHKNDLPGGLELGPVVAIDSETLGLNPFRDRLCLVQLSAG
                     DGVCHAVQFKRGAYAAPNLKRLLADPKVLKLFHFARFDVAMFKRYLGVRTAPVYCTKI
                     ASKLVRTYTDKHGLKDLVKELLAVDLAKEQQCSDWGAAELSERQLAYAANDVAYLHRL
                     KEALDAMLAREERTELARACFDFLPARVDLDLGGWAEPDIFAH"
     gene            2239..>2675
                     /locus_tag="VGC27_00795"
     CDS             2239..>2675
                     /locus_tag="VGC27_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY0281143.1"
                     /translation="MRPASVKAVPSSQPHLAGDLAVARRVLDSAGEAIKSLGEALNGD
                     FARAIDTIMAVKGRVIVSGMGKSGLIGRKIAATLASTGTPAYFVHPAEASHGDLGMIT
                     RADVVLAISNSGGTAELGDLVAHARRFRIPLIVITSRASSALA"
BASE COUNT          447 a          882 c          832 g          514 t
ORIGIN      
        1 gttcgaccag tggccgctgg ctcagaacct gtcctttacc ttcagcgatc gcttcagttt
       61 caccgagaac gacggcatcg tgtttccacg cgacgccgtg cgcaacgatc tgcgcgaggc
      121 ctatttcacc tgggagccga cgacggcgac gttcctggaa ttcggccgcg tcaatctcaa
      181 gaacggcatt gcgctgggct tcaatccgac cgacttcttc aagacccgca gtgcggtggc
      241 tctggcttcg gccgatccct cggcgttgcg cggcgaccgg ctcggtacgc tgatgttgcg
      301 cgcgcaaacc atttgggacg gcggttcggc gacgttcgtt tacgcgccca aactgcacac
      361 gccgaggccg ctggtgggcg ccgcgccaag ctggatcgat ccgggtttcg atcagaccaa
      421 tgcctccgac cgctttctct tcgccctcaa tctcgaactc ggcgacttga gcccgcaggc
      481 gctcgtctat cacgagagcg gccgcaccaa gatcgggctc aatatctccc gtcccatcgg
      541 ccagagcgtc atcgcctatg gcgaatggac gggcggcagc gagccgaacc tgatcgcgca
      601 ggccatcgcc tacggcaagg cgaccgggac gctgccttcc tttgccccgg ttctgccgcc
      661 ggcctcgacg agccacgcct tccaaaacga tctggccgtt ggcgcatcgt ggaccagcga
      721 ggcgaagatc acgctcaatc tcgaatatca tttccatcag gccggatttt ccggcgcgga
      781 ttggaacaat tggttcgaca tcggcgccgc cggtccgctg gccgcctccg agctttggta
      841 cgtccgcggc tttgcctccg ccatgcagca gccgatcagc cggcagcaga ttttctggcg
      901 cgccgattgg caggacgcct tcattcagaa cttcgaactg tcgggaatct cgttcgtcaa
      961 tctcaccgac ggttccggca tggcgcaaat ttccgccaac tacaatttgt ccaacgcctg
     1021 gagcgtcggc ctctatgccg gcggcacttt cggcggccgc cgcagcgaat ggggcagcct
     1081 gcccggcgcc tcgaacgcga ttttccaggt tgtgcggtat ttctgagctt ttcttcgtcg
     1141 ggcacctctt atttcgcgtt gctgccgccc ccttcgcctc gtgctacgcg ctcgtcactt
     1201 ccctcgtgaa cgaggggcag aaacgaagcg cgttgccttt ccgcccccgc gaagcggggg
     1261 aggtgccgag cgagcgatag cgaagtgagg cggagggggc agttatccca aaggacattt
     1321 ccggccttgg ggctccttgg gcatggtggc aacatctcac ggcgacggca ggggccccct
     1381 tccgtcgcgt tgcgggccgg ggcggagtgg cataggttct gccgcacacg ccgatatgga
     1441 caaaatccct tgaaaatcaa actccacaag aacgatttgc ccggcgggct ggagctcggc
     1501 cccgtcgtcg ccatcgacag cgagaccttg ggcctcaatc cgttccgcga ccggctctgc
     1561 ctggtgcagc tttcggccgg cgacggcgtc tgccatgcgg tgcagttcaa gcgcggggct
     1621 tatgccgcgc ccaatctgaa gcgcctcttg gccgatccga aggtgctgaa gctgtttcac
     1681 ttcgcccgct tcgacgtggc gatgttcaag cgctatctgg gggtcaggac cgcgccggtc
     1741 tattgcacca agatcgcctc caagctggtt cggacgtaca ctgacaaaca cgggctgaag
     1801 gacctggtga aggaactgct ggccgtcgat ctcgccaagg agcagcaatg ttccgactgg
     1861 ggcgcggccg aactcagcga acgccagctc gcctatgcgg cgaacgacgt ggcctatctg
     1921 caccggctga aggaggcgct cgacgccatg ctggcgcgcg aagagcggac cgagctggcc
     1981 agggcgtgct tcgatttcct gcccgcgcgg gtggacctcg atctcggcgg ctgggcggaa
     2041 ccggacattt tcgcgcacta agaatccttg tttaccgcct tcttatgagc cgccttgaac
     2101 ctggccgcgc ttttggccac acttggcacg aatttcgcgt cagggccggt ttcctggggt
     2161 cagcctggta gccgccaccg agcgcaggcg ggtttctggg ggcgggaacc ataggtaaca
     2221 aacaagacgg gtgtctgcat gcgtcccgca agcgtcaagg ctgttccttc atcgcaaccg
     2281 catctggcag gcgatctcgc cgtggcgcgg cgggtgctcg attccgccgg cgaggcgatc
     2341 aagtcgctgg gcgaagcgct gaacggggat ttcgcgcgcg ccatcgatac gatcatggcg
     2401 gtcaagggcc gcgtcattgt cagcggcatg ggcaagagcg gtctgatcgg ccgcaagatt
     2461 gccgccacgc tggcctccac cggcacgccg gcctatttcg tccatcccgc cgaagccagc
     2521 cacggcgatc tcggcatgat caccagggcc gacgtggtgc tggcgatttc caattccggc
     2581 ggcaccgccg agctcggcga cctggtcgcc catgcgcggc gcttccgcat cccgctcatc
     2641 gtcatcacca gccgggccag cagcgcgctc gccga
//
LOCUS       DASWPU010000038         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_116460,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000038 DASWPU010000000
VERSION     DASWPU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116460"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VGC27_00800"
     CDS             <1..191
                     /locus_tag="VGC27_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281144.1"
                     /translation="SGDYDLRTVCVSVLPAGLCGREGLGGMIGVDMIGAIRRAYFEEH
                     RPIKEIVRTLSVSRGTVR"
     gene            414..653
                     /locus_tag="VGC27_00805"
                     /pseudo
     CDS             414..653
                     /locus_tag="VGC27_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053284.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            804..1616
                     /locus_tag="VGC27_00810"
     CDS             804..1616
                     /locus_tag="VGC27_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281145.1"
                     /translation="MLPASNLAAAERICGHPRFDKALRRLSRDLATLHRFEPQLVYAV
                     GDRGAFAIVAAGIAFETERGVTLAAVQDFVVGAKLASVGRVRGFFSNLARTGAATPLH
                     DRQDGRRRPWSFGGWLLPTLERWLGAYRDALAEMDGVVDGPTLPLYMRRSVAAYQRQG
                     APLAAAYSGLSLFLDRQLGHLLLLEAVVRAEDTLTDRDIVSTVSRKRFARGLRTSRAH
                     ITLLLADAERRGLLQRTQSGSDLWLADTTSRSARMWFAHELAWVMDVCGAPT"
BASE COUNT          312 a          519 c          507 g          315 t
ORIGIN      
        1 tgtcaggtga ttacgatctc aggacagttt gtgtcagcgt ccttccggca ggtctttgcg
       61 gccgggaagg actggggggg atgatcggcg tggacatgat cggagcgatc cggcgcgcgt
      121 attttgagga acatcgcccg atcaaagaga tcgtgcgcac gctgtcggtg tcgcgcggaa
      181 cggttcgcta ggtcgtccgc agccacgaga cagagttcaa atacgagcgc gaggtccagc
      241 cgacgccgaa gcttggccac ccaagccgcc gttgcaattg cggcgataac tccaaatatc
      301 gaccagttat agagaaccag ttggtccttc agcttgtaat cgccttggat gcggttgagc
      361 ctcaccgcga gaatgcaggt caagctgcct acaactgcca cggggagaaa aagcaggccg
      421 ggcgcttcat atatacgtcg ctgcgtgaat gggcttacgc cagagcctgt gacacctcat
      481 gacaacacgc cgccgaactg cccttctgga cgcaccgcta caactggcat cgccctcatg
      541 gcagcatcgg ctcaaagccg cccatcaggc aactccgacc gacagggaac aacctgttga
      601 gctccacacc taaggcgttt acggcctggt tacttggggc gtttgcgcca taataccgca
      661 attccgcgca atcggcgccg gccgaacagg cgatgcaata aaacgcccat caacattcaa
      721 gacactgctg gaactcagat cttcgtatcc tttcctattg ttcgcgtaga ctgtcgaata
      781 ctgaaaaagt tcaggaaggc gcattgcttc ccgccagcaa cttggctgct gcggaacgta
      841 tttgtggtca cccaagattc gacaaggcac tgcgtcgctt aagccgcgat cttgcgactc
      901 tccaccggtt cgagccgcaa ttggtatatg cggtcggtga tcgtggcgcc ttcgccatcg
      961 tcgcggcggg catcgcgttc gagaccgagc gcggtgtcac cctcgccgcc gttcaggact
     1021 tcgtcgtcgg cgccaaactg gcatcggtgg ggcgcgtccg agggttcttc tcgaaccttg
     1081 cgcggacagg cgcggcgacg ccgctgcacg accggcagga tggacgccgg cgaccctggt
     1141 ccttcggcgg gtggctgttg ccaacgctgg aacgctggct gggcgcgtat cgcgacgcgc
     1201 tcgccgagat ggatggggtg gtggatggac caaccttgcc cctttacatg cggcgctcgg
     1261 tggcggccta tcaacgccaa ggcgccccac tcgcggcggc ctatagcggg ctctcgcttt
     1321 tcctcgatcg tcagctgggc cacctcctgc tgctcgaggc cgtcgtccgg gcggaggaca
     1381 cactgacgga ccgcgatatt gtctcgacgg tctcgcgcaa gcggttcgcc cgcgggctgc
     1441 ggacgagccg cgcgcacatc acgctgctgc tggccgacgc cgaaagacgc ggcctactcc
     1501 aacgcaccca atctggctcc gatctctggc ttgcagacac aaccagtcga tccgcccgca
     1561 tgtggttcgc gcatgaactg gcatgggtga tggacgtctg tggcgcgccc acttgaagca
     1621 ccgtgacgga gtggccgcgc cgcgccgcgc tac
//
LOCUS       DASWPU010000039         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_37094,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000039 DASWPU010000000
VERSION     DASWPU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37094"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1729)
                     /locus_tag="VGC27_00815"
     CDS             complement(158..1729)
                     /locus_tag="VGC27_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEY0281146.1"
                     /translation="MHLSLLDIAVVILYVTFVLVLAQFVSRKREEDTEDGSDPAPRSR
                     ALPWWAIGTSLIAANISAEQIIGMSGSAYALGLAIASYEWTAALVLLAVGKYFLPIFL
                     KNQIYTMPQFLRRRYGRNIQLIMASFWLVLYVFVNLTAILWLGAIAVHTVTGLTLQMS
                     LILLGLVAGNYALYVGLKANAFSDVVQVSMLVLGGLVVVYVALTQVAAAGGSSGLIGG
                     FAILMERMPGHFHMILTPDNPYYKYVPGLSVLLGGMWIIHLSYWGFNQSIIQRALTAK
                     SVREAQKGVVLAAFLKLLIPVLVVVPGIAAAVLLPDLSRPDEAYPMLMTLLPSGLLGL
                     VFVALVAAIVSSMGSTLASIATIFSNDVLKTLNAGASLRLQVIAKRFAAIIALVIAMV
                     AAIPLLSNFDQAFQYIQEYTGFFTPGIVVIFLLGMFWKRATETGALVAAMGSILLSFF
                     YWIFLPAVPFMNRMGYIFLICLGAGVVVSLLEKPKPQSSTIDVAGIDYSTSTSFNIAG
                     LAIILILVALYAFLW"
     gene            complement(1874..>2167)
                     /locus_tag="VGC27_00820"
     CDS             complement(1874..>2167)
                     /locus_tag="VGC27_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281147.1"
                     /translation="GALTAGVSSVALSYLYANKWPYIPAFDSLGHIPFMNRMGYVFLI
                     CLGLAVIVSLMQKPKPQTMTVELADIDYSTSKSFVIASFAVIAVLIALYATWW"
BASE COUNT          521 a          639 c          644 g          363 t
ORIGIN      
        1 cactctcatc gaggatccgg ctgacgggat cgtaacggaa caggcgccgg ccctggatgt
       61 cggtccacca cagccgcgcg tcgcgcacgt tccaaagcgg gccttcgccc agaatgttcc
      121 ggaccggcac catgtcgacc agttcggcca cggccggcta ccataagaac gcgtagagcg
      181 ctacgaggat caaaatgatc gccaaaccgg cgatgttgaa ggaggtgctg gtcgaataat
      241 cgatgcccgc gacatcgatg gtcgaacttt gcggcttggg cttctccaga agcgatacga
      301 ccactcctgc tcccaggcag atcaggaaga tgtatcccat gcgattcatg aaggggacgg
      361 ccggcaggaa tatccaatag aaaaaggaaa gcaggatgga acccatggcg gcaaccaggg
      421 cgcccgtctc cgtcgcgcgc ttccagaaca tgccgagcag gaagatcacc acgatgcccg
      481 gcgtgaagaa gccggtatat tcctggatgt attggaacgc ttgatcgaaa ttgctcaaca
      541 gcgggatggc cgcgaccatc gcaatcacca atgcgatgat ggcggcaaac cgtttggcga
      601 tcacttggag gcgcaagctg gcaccggcgt tgagggtttt gagcacgtcg ttcgagaaga
      661 tcgtggcgat cgaagcgagc gtcgatccca tcgaactgac gatcgcggcg accagcgcca
      721 cgaagaccaa ccccaaaaga ccgctcggca acagggtcat gagcatcggg tacgcttcgt
      781 cgggccgcga cagatcaggc agaagaacgg cggcggcaat gcccggcacc accaccagaa
      841 ccgggatcag aagcttcagg aaggcggcga gaacgacgcc tttctgcgct tcgcgcacgc
      901 ttttcgcggt cagggcgcgc tggatgatgc tctggttgaa gccccaatag gagaggtgga
      961 tgatccacat gccgcccaac agcacggaaa gacccggcac atatttgtag tacggattgt
     1021 ccggggtcag gatcatgtgg aaatgccccg gcatacgctc catgaggatg gcgaacccgc
     1081 cgatcaatcc cgacgagccg ccggccgcgg cgacttgtgt aagggccaca taaaccacca
     1141 ccaatccgcc cagcaccagc atggacactt gcaccacgtc ggagaaggcg ttggccttca
     1201 agccaacgta aagcgcataa ttgcccgcca ccagaccaag cagaatcaag ctcatctgca
     1261 gcgtcagtcc ggtgaccgta tggaccgcaa tggcgcccag ccaaaggatc gccgtcaggt
     1321 tcacgaaaac atagagaaca agccaaaagc tggccatgat cagctggatg ttccggccgt
     1381 aacggcgcct gaggaactgc ggcatcgtgt aaatctggtt cttcaggaag atcggcagga
     1441 agtattttcc caccgccagc agaaccagcg cagccgtcca ctcgtaggac gcgatggcca
     1501 gaccgagcgc ataggccgaa ccggacatgc cgatgatttg ctcggcggaa atgttggccg
     1561 cgatcagcga cgttccgatc gcccaccaag gcagggcgcg gctgcgggga gcgggatcgc
     1621 tgccgtcctc cgtatcttct tctctcttgc gcgacacgaa ttgcgccagc accagaacga
     1681 aggtgacata aaggatcacg acggcgatat cgaggaggga aagatgcatg tttttcacat
     1741 ctttttagat cgaacgtcaa tctagctgat tcgccgttcg accacccgca aggatatcct
     1801 ccggcctgcc ggttccactc ttgctgtctg tttcggggcc gcggcggaac gggcaccgct
     1861 ccgccgcgac cggctaccac caagtcgcgt aaagagcgat cagaacggcg ataaccgcaa
     1921 aacttgcaat gacgaacgat ttgctggtcg aataatcgat gtcggcaagc tcgaccgtca
     1981 tggtctgcgg cttgggcttc tgcatgagcg acacgatgac cgccagtccg agacagatca
     2041 ggaacacgta gcccatgcgg ttcatgaacg gaatatgtcc gagcgagtcg aaggccggga
     2101 tgtacggcca cttgttggcg tagaggtacg acagggccac ggaagaaacc cccgccgtca
     2161 atgcgcc
//
LOCUS       DASWPU010000040         7470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_18660,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000040 DASWPU010000000
VERSION     DASWPU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18660"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..598)
                     /locus_tag="VGC27_00825"
     CDS             complement(95..598)
                     /locus_tag="VGC27_00825"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEY0281148.1"
                     /translation="MTSSDTLLQCVPFARDHSQIALHGDAYTWWDQAAGRFARSTSPK
                     PGAVMVLSGYAGPNRSHLAVVRELVDAREIRVDHANWLNDGAIYIDDPVRDVSPGNDW
                     SQVRIFNLRTRAWGGKTYPVQGFIGPDGAEPQALRLAQNPPSASGQRDEIGALIASDQ
                     DWPDEDN"
     gene            complement(821..2269)
                     /locus_tag="VGC27_00830"
     CDS             complement(821..2269)
                     /locus_tag="VGC27_00830"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0281149.1"
                     /translation="MYGLSLILQCGLAGVLLVLALLSAQLVMLGYFRLFHPERRVRSP
                     HLPDEALPAVLVQLPVRDEGALALRVAAAAARLDWPRDKLEVQLLDDGSAEVHDELAR
                     AVREAVPEGVKVSVLRRNERTGFKAGNLAFGLKHSKAPYVAIFDADFVPPADFLRRTV
                     PTLVADSGLAFVQTRWTHANRNLNWLTRAQGLLLDAHFAVEQEGRFRAGLPISFNGTA
                     GVWRREAIESAGGWTGDTLTEDLDLSMRCLLKGWRTAYLKDVEVPGELPESAAAWRGQ
                     QARWTKGHAQCARKLLPEIWASNLPLWCKAAVSLQMCQFAFYTMAFISAAISLTLMYM
                     GVVYLQSVALLGLTVTAFGLTGSMSYLFLGQKLLGRHRAIGLHRSLLLAIVFPSGLIL
                     ANTRATYEAFFTTHMVFHRTLRPGERYAGGWRGGPELIAGLLLPIFAFAEQAWSAPFF
                     AFAVAGLVSIGAMGFSGGGTRQIASNRVIPRE"
     gene            complement(2521..3657)
                     /locus_tag="VGC27_00835"
     CDS             complement(2521..3657)
                     /locus_tag="VGC27_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0281150.1"
                     /translation="MIGRFSLARVFAMLGKEFIQMRRDRLTLGMLIGIPLMQLFLFGY
                     AINMVPRHLATAVSISDPGPFSNSIVAALSNSSYFDVVKATNSPAQARQMLADGRVSF
                     VIDIPVNFSRDLVRGAKPSLLVEADATDPAAGSYAIAAIPTLVTTALRDDLTGPLAAR
                     AAAAPAFDVVVHSLYNPEATTQYNIVPGLLAVILTLTMVLATAIALTRERERGTYENL
                     LAMPTHPVEIMIGKIVPNIFVGAVQCGLILFVAKFVFAVPMIGSLGLLAATAIFYIAA
                     LLAVGYTFSTLAQNQMQAMQMTFFFLLLSIMLSGFIFPFRGMPDWAQWIGEVMPITHF
                     LRIVRGILLKGNGFIEVWPDVWPMILFLFAAGTVAVGRFRKTLD"
     gene            complement(3654..4577)
                     /locus_tag="VGC27_00840"
     CDS             complement(3654..4577)
                     /locus_tag="VGC27_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0281151.1"
                     /translation="MTNNAIDVRGLTKRFGKKIAVNNVDIAVPEGEVWGFLGPNGSGK
                     TTTIRMLCGLLRADAGEGTCLGYDFRTQSEQIKRETGYMTQKFSFWEDLTIRENLDFV
                     ARLYELPKRRALIDRTLETLGLAERQHQLAADLSGGWKQRMALAACTLHSPKLLLLDE
                     PTAGVDPKARRDFWDQIHTLSNQGITVLVSTHYMDEAERCDRIVYISVGTIVARGSVP
                     EIIAQSGLITFTIEGEGARQLAEKLRGLPGVVFVSFFGAVLHVSGYDRALLEAALAPY
                     RHNPNLAIRETTPSMEDVFIELQQDKGRGNA"
     gene            complement(4574..5392)
                     /locus_tag="VGC27_00845"
     CDS             complement(4574..5392)
                     /locus_tag="VGC27_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0281152.1"
                     /translation="MKRLSIVLALLLAGCGQNDGTTWLGYAEGDYAFVSAPQSGWVTS
                     VKVQRGDWVKNGDLLFTLDDTSQIAARDSAAASIAQAQGQMAQAEANLDLAQKQLVRQ
                     QGLLRANATSKQAYDLAKSSYDSAAAQVSQIAAAENQARAALDNAVYQLAQRRIASRT
                     SGRVQDVYFRQGEYAPAMTPVISLLPPENVYVRFFVPENQFAKIHLGDKVKIGCDGCA
                     DIVATISFIAAREEFTPPVIFSIGSREKLVFKVEARLPAGLKLNPGQPVDVRPL"
     gene            complement(5392..6045)
                     /locus_tag="VGC27_00850"
     CDS             complement(5392..6045)
                     /locus_tag="VGC27_00850"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0281153.1"
                     /translation="MSHTRTLSKPAKGWSRRKQARPGEILEAAMKVFAEKGFAAARME
                     DIAARAGVTKGTIYLYFPSKEEMFKSLARETVHAALAGGAQQVRDFSGTSREMLVTLL
                     SAISAFMQDKDRAMLPKIIMSESGNFPELARFWRSEVVDRALAMVAAAITQGIERGEF
                     RPLPPEHVAKLCVAPIILSLIWRTTFGALETVPTDHQKMFALHLDVLLKGLAPEGAA"
     gene            6186..6710
                     /locus_tag="VGC27_00855"
     CDS             6186..6710
                     /locus_tag="VGC27_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281154.1"
                     /translation="MSSINRLGYAAIAAACVFALAGCQTATPYQPSKGPGKVGYFDQQ
                     LAGNRYRVTFTGNSVTRRDKVEDYLLRRSAEVTLQAGYPYFLFDTRDTKSETTYHSDF
                     VGWPGWRGRGWYWHSWPYGPDPSFDSRPITSYEAYAEIVLLTDDQAKSEARAVNAQEV
                     IDRIGPQPAPEPQP"
     gene            complement(6714..6932)
                     /locus_tag="VGC27_00860"
     CDS             complement(6714..6932)
                     /locus_tag="VGC27_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY0281155.1"
                     /translation="MDTLDLRGLKCPLPALLAKRALVGAPPGASIKIVTDDPLAALDV
                     PHMCHRENFEIVSLARDGDLARMVLRKP"
     gene            7007..>7470
                     /locus_tag="VGC27_00865"
     CDS             7007..>7470
                     /locus_tag="VGC27_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0281156.1"
                     /translation="MLEGLKREYIYIRCLMRTLVRMRHVKPDATRTIVDIVEELAAKK
                     PANIAIFYQDRSVTYRQLDEGANRYARWAQAQGVRRGHAVVLLMENRPDYLMAWLGLL
                     KLGAVAALINTNLKGAPLAHSITIAGARHAILGAELGANYIEAAALLDAAPAA"
BASE COUNT         1434 a         2283 c         2461 g         1292 t
ORIGIN      
        1 ccccgcgagg gcgcggacca taatccggcg tgcgccgggc acaagagggg atgagaccga
       61 ttggcacagg aattttgtaa gacgttgtta ttattcagtt atcttcgtct ggccaatcct
      121 gatcggaagc gatgagggca ccgatttcat ccctctggcc gctggcggag ggcggattct
      181 gggccaggcg cagcgcttgc ggttcggcgc cgtccggacc gatgaaaccc tggacggggt
      241 aggtcttgcc gccccaggct cttgtcctga ggttgaagat ccgcacctgg ctccagtcgt
      301 tgccggggct gacgtcgcgg accggatcgt cgatatagat cgcgccgtcg ttgagccaat
      361 tggcgtgatc gacgcggatt tcgcgcgcgt ccacgagctc gcgcaccacc gccagatggg
      421 aacggttggg gccggcataa ccgctcagaa ccatcaccgc gcccggtttg ggcgaagtgc
      481 tgcgggcgaa gcgccccgcc gcctggtccc accaagtata ggcgtcgccg tggagcgcga
      541 tttgggaatg gtcgcgcgcg aaaggcacgc attgcagcag cgtatcggat gatgtcacgc
      601 cgccaccgtc ataagaggaa gaaggcgcgc ccgcacagcc tgccaggagg gcgcaaaagg
      661 cggctgccgc cgcgagccga acgaaagacc ccaccatcac aagcctcatt tatgctcaag
      721 cggcagcaac cgatgccact gtagcgcaca ggcttcaaag cgaggttagg gcaatcgttc
      781 ggattcgacc gattaaaatt tgaggaaaaa tgtagcaggc tcactcgcgc gggatgactc
      841 ggttggacgc gatctgacgg gtgccgccgc cggaaaagcc catggcgccg atggacacca
      901 gaccggccac cgcgaaggcg aagaacggcg cgctccaggc ctgctcggcg aaggcgaaga
      961 tcggcagcaa caggccggcg atcagttccg gcccgccgcg ccagcctccg gcataacgtt
     1021 caccgggcct cagtgtgcgg tggaaaacca tatgggtagt gaaaaacgct tcgtaggtcg
     1081 cccgcgtgtt ggcgagaatg aggccgctgg gaaagacgat ggcgagcagc agcgagcgat
     1141 gcaggccgat ggcgcgatgg cggccgagca gcttctggcc gaggaacaga tagctcatgc
     1201 ttcccgtgag gccgaaagcg gtgacggtga ggcccagcag cgccacgctc tgaagataga
     1261 cgacgcccat atacatcagc gtcaggctga tcgccgcgct gatgaacgcc atggtgtaga
     1321 aggcgaattg gcacatttgc aggctgaccg ccgccttgca ccacaaaggc aggttgctgg
     1381 cccagatctc tggcagcagc ttgcgggcgc attgggcgtg gcccttggtc cagcgcgcct
     1441 gctggccgcg ccaagcggcg gcgctttcgg gcagctcgcc cggcacttcc acgtctttca
     1501 gatacgcggt gcgccagcct ttcagcaggc agcgcatcga caggtcgaga tcttcggtca
     1561 gcgtgtcgcc ggtccagccg ccggcgcttt cgatggcttc gcggcgccac accccggcgg
     1621 tcccgttgaa cgaaatcggc aagccggcgc ggaagcgtcc ttcctgctcg acggcgaaat
     1681 gggcgtcgag cagaagtcct tgcgcgcgcg tcagccagtt cagattgcga ttggcatggg
     1741 tccagcgcgt ctgcacgaaa gcgagtccgg aatcggcgac cagcgtcggc accgtgcggc
     1801 gcaggaaatc ggcgggcggt acgaaatcgg catcgaagat cgccacatag ggcgccttgg
     1861 aatgtttcag gccgaaggca agattgccgg ccttgaagcc ggtgcgctcg ttgcgcctga
     1921 ggacggagac cttgacaccc tcgggcaccg cctcccttac cgcgcgcgcc agttcgtcgt
     1981 gaacctcggc gctgccgtcg tcgagcagct ggacttcgag cttgtcgcgc ggccagtcga
     2041 gacgggccgc cgccgcggcc acgcgcaagg ccaaggcgcc ttcgtcgcgc accggcaatt
     2101 gcaccagcac cgcaggcaac gcctcgtcgg gcaagtgtgg gctgcgcacg cgccgttcgg
     2161 gatggaagag acggaaataa ccaagcatga ccaactgagc ggaaagcagg gccagcacca
     2221 gcaggacgcc ggcaagcccg cattgcaaga tcagcgataa gccatacatg cggaccgcac
     2281 tgccccccat accgttccga agtacacgcc gcctggccgg cggactggcg gaaaccccca
     2341 agggcttaaa gacctgcccc ttcaggcgga ggtgaatttg ttacgcagtt ttatctttcg
     2401 caagaggcga gcgccagtct caggggccga tttcccttaa taatggttcg cgaaatcgcc
     2461 cccaattccg gctttggccg gttttcctgc ggcgaaccgc tgcatgggcg ggtgaaaggc
     2521 tcaatccagc gtcttgcgga aacggccgac cgccacggtt ccggccgcga acaaaaagag
     2581 aatcatgggc cagacatcgg gccaaacctc gatgaagccg ttgcccttga gcaggatgcc
     2641 ccggacgatg cgcaggaaat gggtgatcgg catgacctct ccgatccatt gcgcccagtc
     2701 cggcatgccg cggaagggaa agatgaagcc cgacagcatg atcgacagca gaaggaagaa
     2761 aaacgtcatc tgcatcgcct gcatctggtt ttgcgccagg gtggagaagg tgtagcccac
     2821 cgccaacagc gcggcgatgt agaaaatggc cgtcgccgcc aacaggccca acgacccgat
     2881 catcggtacg gcgaacacga atttggcgac gaacaggatg aggccgcact gcaccgcacc
     2941 gacgaaaatg ttgggaacga tcttgccgat catgatttcg accgggtgcg tcggcatggc
     3001 aagcaggttt tcgtaggtgc cgcgttcgcg ctcgcgcgtc agggcgatcg cggtcgccag
     3061 caccatggtc aaggtgagaa tcacggccaa caggcccggc acgatattgt attgggtggt
     3121 ggcttccgga ttgtagagac tgtgcaccac gacgtcgaac gcaggcgcgg cggcggcgcg
     3181 cgccgccaac ggcccggtga gatcgtcgcg cagcgccgtg gtgacgagcg tgggaatggc
     3241 ggcgatggca taagaaccgg cggcgggatc ggtggcgtcg gcttccacca gcagcgatgg
     3301 cttcgcaccg cgcaccaggt cgcgcgagaa attgaccggg atgtcgatga cgaaggaaac
     3361 ccgcccgtcg gccagcatct ggcgcgcctg cgccggcgag ttggtcgctt tgacaacgtc
     3421 gaaatagctg gaattgctca gcgcggcgac gatggaattc gagaacggac ccggatcgga
     3481 gatcgacacc gccgtcgcca gatgccgggg caccatgttg atggcgtagc cgaacaggaa
     3541 caactgcatc agcggaatgc cgatcagcat gccgagcgtc agccggtcgc ggcgcatctg
     3601 gatgaattcc ttgcccagca tggcgaagac gcgggcgagc gagaaccgtc cgatcatgcg
     3661 ttccccctgc ccttgtcctg ctgcaactcg atgaagacgt cttccatgct gggcgtggtt
     3721 tcgcggatcg ccagattggg gttgtggcga tagggcgcca acgccgcctc aagcagggcg
     3781 cggtcatagc cgctgacatg caggacggcg ccgaagaagg agacgaagac gacgccgggc
     3841 agcccgcgca gtttttccgc gagctggcgg gcgccttcgc cttcgatggt gaaggtgatg
     3901 aggcccgatt gggcgataat ctcgggcacc gagccgcgcg ccacgatagt gccgacgctg
     3961 atatagacga tgcggtcgca gcgttcggct tcgtccatgt aatgggtgct gaccagcacg
     4021 gtgatgcctt ggttcgacag agtgtgaatc tggtcccaga agtcgcgccg ggccttggga
     4081 tcgacgccgg cggtgggttc gtcgagcaag agcagcttgg gcgaatgcaa ggtgcaggcg
     4141 gcgagcgcca tgcgctgttt ccagccgccc gaaagatcgg ccgcgagctg gtgctggcgc
     4201 tccgcgagac cgagcgtttc gagagtccgg tcgatcaggg cgcggcgctt gggcagttcg
     4261 taaagccggg cgacgaaatc gagattctcg cggatcgtca ggtcttccca gaagctgaac
     4321 ttctgggtca tgtagccggt ttcgcgcttg atctgttcgg actgggtgcg gaaatcgtaa
     4381 ccgaggcagg tgccttcgcc ggcgtcggcg cgcaacagcc cgcacagcat gcggatcgtc
     4441 gtggtcttgc ccgaaccgtt gggcccgagg aagccccaca cctcgccttc gggcacggcg
     4501 atgtccacgt tattgaccgc gatcttcttg ccgaagcgct tggtcaggcc gcgaacgtcg
     4561 atggcgttgt tggtcacagc gggcgcacgt ccacaggttg gccgggatta agtttcaagc
     4621 ccgcaggcag gcgcgcctcc accttgaaga cgagcttttc gcggctgccg atggagaaga
     4681 tcacgggcgg agtgaattcc tcgcgggcgg cgatgaagct gatggtcgcg acgatgtccg
     4741 cgcagccgtc gcagccgatc ttcaccttgt cgccgagatg aattttggcg aactgattct
     4801 caggcacgaa gaagcgcaca tagacgttct cgggcggcag cagggaaatc accggagtca
     4861 tggcgggggc gtattcgcct tggcggaaat aaacgtcctg cacgcgcccc gaggtgcgcg
     4921 aagcgatccg gcgctgggca agctgataga cggcgttgtc gagcgcggcg cgggcctggt
     4981 tttccgccgc ggcaatctgg gagacctggg cggcggcgct gtcgtagctc gatttcgcca
     5041 gatcataggc ttgtttcgag gtggcgttgg cgcgcagcaa gccttgctgg cggaccagtt
     5101 gcttctgggc gagatcgaga ttggcctcgg cctgggccat ttggccttgc gcctgggcga
     5161 tggaagccgc cgccgaatcg cgcgccgcga tttggctggt gtcgtcgaga gtgaagagca
     5221 gatcgccgtt cttcacccag tcgccgcgct gaaccttgac ggaggtgacc cagccggact
     5281 gcggcgcgga aacgaaggca tagtcgcctt cggcgtagcc cagccaggtg gtgccgtcat
     5341 tttgtccgca tcccgcaagc agcaaggcca gcacaatgct caatcgcttc atcatgcggc
     5401 tccttcgggc gccaaccctt tcaacagaac atccaaatgc agggcgaaca ttttctgatg
     5461 atcggtgggc accgtctcca gcgccccgaa ggtcgtgcgc cagatgaggc tcaatataat
     5521 cggggcgacg catagtttgg cgacatgctc gggcggcagc gggcggaatt cgccgcgctc
     5581 tatgccctgg gtgatcgcag ccgccaccat cgccagcgcc cggtccacga cttcgctgcg
     5641 ccagaagcgc gccagctcgg ggaaattgcc gctttccgac atgatgatct tgggcagcat
     5701 ggcgcggtct ttgtcctgca taaacgccga gatggccgac agcagcgtca ccagcatctc
     5761 gcgcgaggtg ccgctgaaat cgcgcacctg ttgggcgccc cccgccagcg ccgcgtggac
     5821 cgtttcgcgc gccagcgact tgaacatctc ctccttgctg gggaagtaga gatagatcgt
     5881 gcctttggtg accccggcgc gcgccgcgat gtcttccatc cgggcggcgg caaaaccttt
     5941 ttccgcgaat actttcatcg ccgcttccag aatctcgcct gggcgggcct gtttgcggcg
     6001 gctccagcct ttcgccggct tggaaagcgt gcgggtatgg ctcatggtca gtatattaat
     6061 aactgaccag tcatttatca agtgcaaaaa agcctctggt gcaaatgaat tttccggccg
     6121 cgaaaaagtt cgggtcaagc cgccttggaa tttgctaaac taaggccagc gcaaggagat
     6181 ttcggatgtc cagcatcaac cgcctaggct acgcagccat tgccgccgcc tgtgtgttcg
     6241 ctctggccgg ttgccagacc gccacgccct atcagcccag caaggggcct ggcaaggtgg
     6301 gctatttcga tcagcaactg gccggcaacc gctatcgggt gaccttcacc ggcaactccg
     6361 tcacgcggcg cgacaaggtg gaagattatc tgctgcggcg ttcggcggaa gtaacgctgc
     6421 aggcgggcta tccctatttc ctgttcgaca cgcgcgatac caaaagcgag accacctatc
     6481 acagcgattt cgtcggctgg ccgggctggc gcggccgcgg ctggtattgg cactcttggc
     6541 cttacggacc cgatcccagt ttcgatagcc gcccgatcac cagctacgag gcttatgccg
     6601 agatcgtgct gctcaccgac gatcaggcga agagcgaggc gcgtgcggtc aacgcccaag
     6661 aagtgatcga ccgtatcggc ccgcagccgg cacccgagcc tcagccttaa agttcacggc
     6721 ttcctgagca ccatgcgcgc gagatcgccg tcgcgcgcga ggctgacgat ctcgaagttc
     6781 tcgcggtggc acatatgggg cacgtcgagg gcggcgagcg gatcgtcggt gacgatcttg
     6841 atagaggcgc cgggcggggc gccgacgagg gcgcgcttgg ccagcagtgc cggcagcggg
     6901 catttcaggc ctctgagatc gagcgtgtcc atccggacct cgttgccttg acgctgaacg
     6961 actacactct aacctgcgcg gccggggaga tggtaccggg gctgcgatgc tggaaggcct
     7021 caagcgcgaa tatatctaca ttcgctgcct gatgcggacc ctggtccgca tgcgccatgt
     7081 caagcccgac gccacgcgca ccatcgtcga tatcgtcgag gaattggcgg cgaaaaagcc
     7141 ggcgaacatc gccatcttct atcaggaccg cagcgtcacc tatcgccagc tcgacgaggg
     7201 cgcgaaccgt tacgccagat gggcccaggc ccagggcgtg aggcgcggcc atgccgtggt
     7261 gctgctgatg gagaaccgcc cggactatct gatggcctgg ctcggtctgc tcaagctcgg
     7321 cgcggtggcg gcgctgatca acaccaatct gaagggcgcg ccgctggccc attccatcac
     7381 catcgccggt gcgcgccacg ccatcctcgg cgcagagctg ggcgcgaact atattgaagc
     7441 cgccgcgctg ctcgatgccg cgccggcggc
//
LOCUS       DASWPU010000041         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_67655,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000041 DASWPU010000000
VERSION     DASWPU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67655"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..707)
                     /locus_tag="VGC27_00870"
     CDS             complement(330..707)
                     /locus_tag="VGC27_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281157.1"
                     /translation="MMRKLLLAAMLTVLMPAAANATAWIAACYDGKNLQYNQIIGGKG
                     TLFLQKDDGNYQSLALVQSSYEGNVVCGVADDKQAQAGGLYIKVCADKANNTITVKYK
                     DPTGKGLSIDDTAPYCGAAVTVH"
     gene            complement(867..1241)
                     /locus_tag="VGC27_00875"
     CDS             complement(867..1241)
                     /locus_tag="VGC27_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281158.1"
                     /translation="MRKFLLIAALTALMPAAANAATWVATCTDGKNVQYNQTVGGAGF
                     LYLKTDMGVYQVARLSQTHASRAAVCCTVYGNAPPGAEPITQVCADKVRKVIHLKYQN
                     PAKPGSSVVGAGTFCSATVTVQ"
     gene            complement(1397..>2528)
                     /locus_tag="VGC27_00880"
     CDS             complement(1397..>2528)
                     /locus_tag="VGC27_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0281159.1"
                     /translation="TDDPRAAPGHVVFPRRFHALHAATGAAIGTQSAMQYRPLGNTGM
                     FISELCLGTMTFGGKGPIYGKMGNVQQAEATALVARSLEAGVNFLDTADVYSFGVSEQ
                     MTGQALKDIGVKRSDVVIATKVFGRMGEGPNDTGLARGHIMDAVEKSLERLQTDHIDL
                     YQVHAQDLSTPVEEVMRALDDLVSRGLVRYAGCSNWQAWRIMQAQGIADKRGWARFET
                     LQAYYSLAGRGLERELVPMMADQKIGLLVWSPLAGGILSGKFGPNSTPEGARRTTFDF
                     PPVDKERVWPIVDAMREIGKPKGASPARVALAWVLSKPFVTSVIMGVKTPEQLDDNLA
                     ASELALSPGDIKRLDDLSALEPEYPGWMVERQNEGRRSGTNK"
BASE COUNT          418 a          783 c          797 g          530 t
ORIGIN      
        1 cgcgcgcggc tacacgcgcg ccgaagagct gctgcaactc taccgcacgg actggggccg
       61 cagcctcgat ccgctgttcg ccgaatataa tttcctctga agctggggtt tggcctcgat
      121 tgcctatggc ctaatcaatt acttgtcatg gcccggcttg accgggccat ccagcaggcg
      181 aggtccgtag cgacagcgta cggacgtgag ccaaagagtg ctgcgatcac ctggatggcc
      241 tgcgttcata cgctgtcgct atgaaccggg ccatgacagt tttgataagg atcttcttat
      301 tgagtacgga cccttggcgg cctgacgggt cagtgaaccg tcaccgccgc gccgcagtac
      361 ggagccgtgt cgtcgatgga cagtcctttg cccgtcgggt ccttgtattt cacggtgatg
      421 gtgttgttcg ctttgtcggc gcacaccttg atgtacagcc cgccggcttg cgcctgtttg
      481 tcgtcggcca cgccgcagac cacattgcct tcataagagc tttggacaag ggcaaggctc
      541 tgatagttgc cgtcgtcctt ctgcagaaag agagtgcctt tgccgccgat gatttgattg
      601 tattgcaggt tcttgccgtc gtagcaggcc gcgatccagg ccgtggcgtt cgcggcggcg
      661 ggcatcagca cggtcaacat agcggcgagc agcaatttac gcatcatgat ctccgtcgga
      721 tgcgaacgcg cctgcgcgtc cgtttccccg gcgattgttg cgccgcctcg cgtcgatggc
      781 aacccttcaa aggacggtgg cgctgttcgg caaaaaaaag agcggctgtg cgccgctctt
      841 gcaggtttgc aaggggtcgc cgcccgttac tggaccgtca ccgtggcgga acagaaagtt
      901 cccgcaccta cgacggagct gcccggtttt gccggattct ggtatttcaa atggatgacc
      961 ttgcgcactt tgtcggcgca gacttgcgtg atgggttcgg cgccgggagg tgcgttgcca
     1021 tagaccgtgc agcagactgc cgctctgctg gcgtgggtct gggaaagacg ggctacttga
     1081 taaacgccca tgtcggtttt cagatagaga aatcccgcgc cgccgactgt ctgattatat
     1141 tggacgttct tgccgtcggt gcaggtcgcc acccatgtcg cggcgttcgc ggcggctggc
     1201 atcaatgcgg tcaatgccgc gatgagaagg aatttgcgca tcctgaactc cccgagtccc
     1261 ggcggatgcg gacgcacgag aaccgtgccg agcaacggtc ctcgaccgtg tgcccgaata
     1321 ttacaccctc ggccggcaat tgtcccgttt ggattttgtc gagcctttca ggcgtcggct
     1381 cctgagtcgc tgtgcgctat ttattcgtgc cggagcggcg cccttcgttc tggcgctcga
     1441 ccatccagcc cggatattcc ggttcgagcg cgctgagatc gtccagccgc ttgatgtcgc
     1501 cgggcgacag cgccagctcc gaggccgcga gattgtcgtc gagctgttcc ggcgtcttga
     1561 cgcccatgat gacgctggtg acgaacggct tcgaaaggac ccaggccagc gccacgcgcg
     1621 ccgggctggc gcccttcggc ttgccgattt cgcgcatggc atcgacgatc ggccagacgc
     1681 gttccttgtc gaccggcggg aagtcgaagg tggtgcgccg tgcgccttcg ggcgtgctgt
     1741 tcggaccgaa cttgcccgac aggatgccgc cggccagcgg gctccacacc agaagcccta
     1801 tcttctggtc tgccatcatc ggcacgagtt cgcgctccag gccgcgcccg gccagggagt
     1861 aataggcctg caaggtctcg aaccgcgccc agccgcgttt gtcggcgata ccttgcgcct
     1921 gcatgatccg ccaagcctgc cagtttgagc agccggcgta gcgcaccaga ccgcggctca
     1981 ccagatcgtc gagcgcgcgc atcacttcct cgaccggcgt ggaaagatcc tgggcgtgga
     2041 cttggtagag gtcgatgtga tcggtctgca aacgctccag gcttttttcc accgcgtcca
     2101 tgatgtggcc gcgggccagc cccgtatcgt tcggcccctc tcccatgcgg ccgaacacct
     2161 tggtggcgat gacgacgtcc gagcgcttga cgccgatgtc cttgagcgcc tgtccggtca
     2221 tctgctccga cacgccgaac gaatagacgt cggcggtatc gaggaagttg acgccggctt
     2281 ccagcgagcg cgcgacgagg gcggtcgcct ccgcctgctg cacgttgccc atctttccat
     2341 agatgggacc ctttccgccg aacgtcatgg tgccgagaca cagctcggaa atgaacatgc
     2401 cggtgtttcc gagcgggcgg tattgcatcg cgctttgcgt tcctatggct gcgccggtgg
     2461 cggcgtgtag ggcgtgaaat cgccgaggga acacgacgtg ccccggcgca gcacgcggat
     2521 catctgtg
//
LOCUS       DASWPU010000042         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_24729,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000042 DASWPU010000000
VERSION     DASWPU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24729"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VGC27_00885"
     CDS             complement(<1..848)
                     /locus_tag="VGC27_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281160.1"
                     /translation="MLTPDKAGLLQSFLGGLPEPMAARLAKAVEVDRLVEGKGLPHEM
                     ILDGLRPALRKSDAPQRILNPLRLFCLPFEDLLFNGARKEKQKGRVLRSSIMPVWNWL
                     GRTVIPETARSYSGEIKALVLATKLEEALECASKFWPLAGEALRTALAANPKGARAAL
                     GGDLVVGDAEDISLLLCAGPAMVEIQHMLPKPVPVLTDELLWSLRRIYDRVSESALDS
                     APYVPVVVMARLAKPWEALKLALLITHQTHDTLISSTDMGLVGDILFNRLEECRMAIH
                     AVRHPN"
     gene            complement(1130..1414)
                     /locus_tag="VGC27_00890"
     CDS             complement(1130..1414)
                     /locus_tag="VGC27_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY0281161.1"
                     /translation="METRPVIVVSYRNTLYGPCLVVPTSTDPQEGNKWAHKLSVTFDD
                     VQSWAVCNQPSTIAPSRLSQFKGRIPLVPQADFDAVLRLLLQWIPAPRDH"
     gene            complement(1530..2912)
                     /locus_tag="VGC27_00895"
     CDS             complement(1530..2912)
                     /locus_tag="VGC27_00895"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY0281162.1"
                     /translation="MPFWARLKLDSLAVRLILAAVVWAMVGLAAGGYVLSDAFVSAMR
                     DNFDTALQVDLDGMIAAAEPDANGEVELREHFLNRRFARVYSGLYWQIVPQKPGARVQ
                     ISRSLFDRTIQIRNEYKTGMLVWGTAEGPDNQHLRVLARRVSFPISATAEVGDSQTYT
                     FMVAGDLASVDNDVAEFNGMLIWSFVGFLLGLVVAIFIQVRVGLSPLRRAREALARIR
                     SGAARRLEGNFPAEIAPLAAELNSLIEHSAEVVGRARTHVSNLAHFLKTPLTVLASEA
                     EAAPGPLAEAVKRQVDVMRRQVDHYLARARAAGALDVLGNSAPVAPVLDDLARVLERI
                     HEARGIEIEVDCPRPVAFRGDRQDLEEMAGNLMDNACKWAATRVVVTVRGEGTRLVLT
                     VEDDGPGLAAADLGRVLERGERLDESVPGSGLGLSIVRDISKLYEGGLELKQSALGGL
                     CAVLDLPAIG"
     gene            complement(2912..>3190)
                     /locus_tag="VGC27_00900"
     CDS             complement(2912..>3190)
                     /locus_tag="VGC27_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281163.1"
                     /translation="SSKRCVALAIALTIRRLLARTARRTVLDWLVPSANVSWPAKAGH
                     PGDEERTRLRKISALPAKAIGRQLGGPLLVRHSFSDGGLRAVTKEFNM"
BASE COUNT          629 a         1044 c          994 g          523 t
ORIGIN      
        1 aaattggggt ggcggacggc atggatcgcc atgcggcact cctccagccg gttgaacagg
       61 atgtcgccga ccagtcccat gtcggtcgag gaaatcagcg tgtcatgggt ctggtgcgtg
      121 atcagcaggg cgagcttcaa ggcttcccag ggcttggcca ggcgcgccat caccaccacc
      181 ggaacatagg gcgcggagtc gagcgcgctt tcgctcaccc ggtcgtagat gcgacgcagg
      241 ctccacagaa gctcgtcggt cagcaccggc accggcttgg gcagcatatg ctggatctcg
      301 accatcgccg gaccggcgca caagagcagc gagatgtcct cggcgtcgcc gacaacgaga
      361 tcgccgccca gcgccgcgcg cgcgcccttg gggttcgcgg cgagcgccgt gcgcagcgct
      421 tctcccgcca gcggccagaa tttgctggcg cattcgagcg cctcttccag cttggtggcg
      481 agcaccaggg ctttgatctc gccgctatag gaccgggccg tttcggggat caccgtccgg
      541 ccgagccagt tccacaccgg catgatgctc gaacgcagga cgcgcccctt ttgcttttcc
      601 ttgcgcgccc cgttgaacag aagatcctcg aacggcaggc agaacaggcg aagcggattg
      661 aggatacgct ggggcgcatc gcttttgcgc agcgccggac gcaggccgtc gagaatcatc
      721 tcgtgcggca ggcccttgcc ctcgaccagc cggtcgactt ccaccgcctt ggccaggcgc
      781 gcggccatgg gctcgggcaa accgccgagg aaagattgca gcaaaccggc cttatcgggc
      841 gtcaacatcg cagttccgtc gcgtccccaa acacactgct cgcagaggag aatagcaacc
      901 catccttaac gggccattgc cgggcgaaat caaccgattg cgggcagatc gaggacggcg
      961 cagagcacgc ccaacgggct ttgcttaagc tccagccgcc gctgtatagc ccagaaaagc
     1021 ctaaggggag gctttgcggc ctccccttgg tcaagtctct cccttgcggg cgacggtcac
     1081 ccttacgggg ccgcatgtac aaacaacata gacttaaaag acctaaattt caatgatcgc
     1141 gtggggccgg tatccattgc aggaggagcc gcaaaactgc gtcgaaatcg gcttgcggca
     1201 ccagcggaat ccggcccttg aactggctta ggcggctcgg cgcgatggtg gagggctgat
     1261 tgcagacggc ccacgattgc acatcgtcaa acgtcaccga gagcttgtgc gcccacttgt
     1321 tgccctcctg cggatcggtg gaggtcggga cgacaaggca cggcccgtag agcgtgttcc
     1381 tataggaaac gacgattacc ggccgcgttt ccagaactca ggcagttggg cgtcgaccca
     1441 aaaatcacac caatataaac cgcgaatctt aggagcgccg ataagacgag gtttgatccg
     1501 cggtggtttc tgatcggcca tataatcctt caccctatcg cgggcagatc gaggacggcg
     1561 caaagcccgc ccagagcgct ttgcttaagc tccagcccgc cttcgtaaag cttggaaata
     1621 tcgcgcacaa tcgacagccc caggcccgag ccggggacgc tttcgtccag cctttcgccc
     1681 cgctccagca cccggccgag gtcggcggcg gcaaggccag gcccgtcgtc ctcaacggtg
     1741 agaacaagac gcgtgccctc gccccgcacc gtcaccacga cgcgcgtggc ggcccatttg
     1801 caggcattgt ccatcagatt gcccgccatc tcctccagat cctggcggtc gccgcggaag
     1861 gcgacgggcc gaggacaatc aacctcgatc tcgatcccgc gcgcctcgtg gatgcgttcg
     1921 agcacccgcg ccagatcgtc cagcaccggg gcgacgggcg cgctgttgcc gagcacgtct
     1981 agcgcgccgg cggcgcgcgc ccgcgccagg taatgatcga cctggcggcg catgacatcg
     2041 acctggcgct tcaccgcctc ggcgagcgga ccgggagcgg cctcggcctc gctggccagc
     2101 acggtcaggg gtgttttcag gaaatgcgcc agattggaga cgtgggtgcg ggcgcggccc
     2161 accacttcgg cggaatgttc gatcaggctg ttgagttcgg cggccagcgg cgcgatctcg
     2221 gcggggaagt tgccttccaa ccgccgcgcg gcgccgctgc ggatgcgcgc caaagcctcg
     2281 cgcgcgcgcc gcagaggcga cagcccgacg cgaacctgga tgaagatcgc caccaccagc
     2341 cccagcagaa agccgacgaa cgaccagatc agcatgccgt tgaactccgc cacgtcatta
     2401 tcgaccgagg caagatcgcc cgcgaccatg aaggtgtagg tctggctgtc gccgacttcc
     2461 gccgtggcgg agatgggaaa cgaaacgcgg cgcgccagca cgcgcaggtg ctggttgtcg
     2521 ggaccctcgg ccgtacccca gaccaacatg cccgtcttgt attcgttccg tatttgaatc
     2581 gtcctgtcga acagcgagcg ggagatctgc acccgggcgc cgggtttctg cggtacgatc
     2641 tgccaataaa ggccggaata gacacgcgcg aaacggcgat tgaggaaatg ctcccgaagc
     2701 tcgacttcgc cgttggcatc gggctcggcg gcggcgatca tgccgtcgag atcgacctgc
     2761 agcgcggtat cgaaattgtc gcgcatggcg gaaacgaacg catcggacag cacatagccg
     2821 cccgccgcca gaccgaccat cgcccaaacc acggcggcca ggatcagccg caccgcgagc
     2881 gaatcgagtt tgaggcgagc ccagaacggc atcacatatt aaactccttt gtcacggcac
     2941 gcaatccgcc atcgctgaag ctatgacgga caagaagcgg gccgcccagt tgacgcccga
     3001 tagctttcgc aggcaacgca gagattttcc gtagccgcgt gcgctcttca tcacctggat
     3061 ggcccgcttt tgcgggccat gacacgttgg cgctaggaac cagccagtct aaaaccgtcc
     3121 tacgcgctgt tcttgcgagg agccgccgga tcgtcaaggc aatagccaag gccacgcacc
     3181 gtttggatga
//
LOCUS       DASWPU010000043         1717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_18682,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000043 DASWPU010000000
VERSION     DASWPU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18682"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VGC27_00905"
     CDS             <1..703
                     /locus_tag="VGC27_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEY0281164.1"
                     /translation="IGVHFATAPASEGPPTACSLIAVAPDGQRSMNTHLGAARDLSPD
                     DIKEAEIAAAGILYIEGYLWDAEAAKAASRKAIAIAKAAGTKVAFTLSDPFCVGRFRE
                     EFLDLLANDLDILFANEEEALALFETESFDEVQRQMGFWGGVAAVTRSEKGCVVVRGQ
                     EVHAVEAAPAPRVVDTTGAGDQFAAGFLYGLARGKNLRVCGQLGALAAGEIISHFGAR
                     PETSLEKLAARAALL"
     gene            complement(707..1204)
                     /locus_tag="VGC27_00910"
     CDS             complement(707..1204)
                     /locus_tag="VGC27_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY0281165.1"
                     /translation="MSRRRTTDEERELFRAVLAGKTLPKPLRPQPKAARAKAVEKKAP
                     AVLTGVDGGTGERLRRGAIVPDSKLDLHGLTESAAYRALTFFLQAAHKRGDRLVLVVT
                     GKGAPAASEPFALEPRSRGVLKTMVPRWLGEPQFARLIADMRGAHRRHGGEGALYLYL
                     RKPKA"
     gene            complement(1201..>1717)
                     /locus_tag="VGC27_00915"
     CDS             complement(1201..>1717)
                     /locus_tag="VGC27_00915"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEY0281166.1"
                     /translation="AAQNGQIYTPIGRTLKQRGALKPADISMPSIRAWLRAHPGEARE
                     VMQTNASYVFFKEEPVGDPALGAKGAQGVALTPGASLAVDTRLHLLGAPYFVSTSLPD
                     TKPLRRLFVAQDSGGAIRGPVRGDIFFGFGTEAERLAGTMNRSGRLYVLLPKPVAARL
                     ADRTDYPASAP"
BASE COUNT          279 a          550 c          589 g          299 t
ORIGIN      
        1 catcggcgtc cacttcgcga ccgcgcccgc cagcgaggga ccaccgacgg cctgttcctt
       61 gattgcggtc gcgccggacg gccagcgcag catgaatacg catctgggcg ccgcccgcga
      121 tctgtctccg gacgacatca aggaagccga gattgccgcc gccgggatcc tctatatcga
      181 gggctatttg tgggacgccg aagcggccaa ggcggcttcg cgcaaggcga tcgcgatcgc
      241 caaggcggcg ggaaccaaag tcgcctttac cttgtccgat ccgttctgcg tcgggcgttt
      301 ccgcgaggag tttctcgact tgctggcgaa cgacctcgac atcctgttcg ccaacgaaga
      361 ggaagccctg gcgctgttcg aaaccgaaag cttcgacgaa gtgcagcggc agatggggtt
      421 ttggggcggt gtcgcggcgg tgacgcgttc ggaaaagggt tgcgtcgtgg tgcgggggca
      481 ggaagttcac gccgtcgaag ccgcgccggc tccgcgcgtc gtcgatacca ccggcgccgg
      541 cgaccagttc gcggcgggat ttctctatgg actggcgcgc ggcaagaacc tgcgcgtctg
      601 cggacaattg ggcgcgctgg cggcgggcga gatcatctcc catttcggcg cgcggccgga
      661 gacttcgctg gagaagcttg ccgcgagggc cgcgctgctt tgaagctcag gctttgggtt
      721 tgcgaagata gagatagagc gcgccttcgc cgccgtggcg gcgatgcgcg ccgcgcatgt
      781 cggcaatcag gcgggcgaat tgcggttcgc cgagccagcg cggcaccatc gtcttgagca
      841 cgccgcgcga acgcggttcg agggcgaacg gctcgctcgc ggcgggggcg ccttttccgg
      901 tgacgaccag aaccagcctg tcgccgcgct tatgggccgc ctgaagaaaa aaggtgaggg
      961 cgcgatacgc cgcgctttcc gtcaagccat gcaggtcgag cttggaatcg ggaacgatag
     1021 cgccgcggcg cagccgttcg ccggttcctc cgtccacgcc ggtgagcact gccggagctt
     1081 tcttttccac cgctttggcg cgggcggctt tgggctgcgg ccgcaaaggt ttgggcagcg
     1141 tcttccccgc caagacggcg cgaaaaagct cgcgttcctc gtcggtggtg cggcggcggc
     1201 tcatggcgcg ctcgcaggat aatcggttct atccgccagc cgtgcggcca ccggcttggg
     1261 caacagcaca taaagccggc cgctccggtt catcgtgccc gccagcctct ccgcctccgt
     1321 gccaaaaccg aaaaagatgt cgccgcgaac cgggccgcgg atcgccccgc cgctgtcctg
     1381 ggcaacgaaa aggcgccgca acggtttggt gtcgggcaag ctggtggaaa cgaaataggg
     1441 cgcgcccaac aggtgcagcc tggtatcgac ggccagactt gctcccggcg tcaacgccac
     1501 gccttgagcg cccttggccc ccagcgccgg atcgccgacc ggctcttcct tgaagaagac
     1561 ataggacgca ttggtttgca tcacttcgcg ggcttctccg ggatgggcgc gcagccaagc
     1621 gcgaatgctc ggcatggaaa tgtccgccgg cttgagcgca ccgcgctgtt tcagcgtccg
     1681 cccgatgggc gtgtagattt gtccgttttg cgccgcg
//
LOCUS       DASWPU010000044         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_6505, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000044 DASWPU010000000
VERSION     DASWPU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6505"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VGC27_00920"
     CDS             <1..1186
                     /locus_tag="VGC27_00920"
                     /inference="COORDINATES: protein motif:HMM:NF018010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEY0281167.1"
                     /translation="GQQLPYQGMQQPAVPNLDEDSSVSGRTGGSNDVGRLSQLAAQLR
                     VDPLVLVNFLQDHAVNVDGAVRVGGLYFVGPDADLQSLLLAAGGVESCADRGTIEVIS
                     TNIDSRAGSSQTRRRTVSLADAGGTDYIVSPHDEVRVAKVFTDVGIGSVKLQGQFRHA
                     GTYQIVRGERLSDVLMRAGGLTDTAYPYGTVLLRRSAAEKEREAFRREATEINDQLLA
                     AMSRRDPMAKLPPEAFTALQSYVNDLKNQPALGRVTITADPTVLAANPSLDPLLEPGD
                     VVFVPQRPYSVYVLGQVLQPGTVPFRANMSVSDYIAQAGGYAQYADKSQTFVVLPDGS
                     ARQAGTSWFDFGGNKIPPGSTVFVSREIAGFDWRQTILDSASIFRDFATTAASLAVLS
                     KQ"
     gene            1540..>1799
                     /locus_tag="VGC27_00925"
     CDS             1540..>1799
                     /locus_tag="VGC27_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281168.1"
                     /translation="MFKRFGAAALAASLLVSSTAFAAANANQGALAQGKPATVKQAES
                     YQGQKYVWWVLGAGVVIGGVALVASGDGHGLVGTPCPFGGCPT"
BASE COUNT          331 a          496 c          572 g          400 t
ORIGIN      
        1 cggccagcaa ctgccgtatc agggcatgca acaacccgcg gtgcccaatc tggacgagga
       61 ctcatcggta tcgggacgga cgggaggcag caacgacgtc ggccgcctga gccagcttgc
      121 cgctcaactg cgagtcgatc cgttggttct ggtcaatttc ctgcaggatc acgccgtaaa
      181 tgtcgacggc gccgtaagag ttgggggcct gtatttcgtc ggcccggatg ccgatcttca
      241 atcgctgctg ctggctgccg gcggcgtaga gagctgtgcc gacaggggca cgatcgaagt
      301 gatttcgaca aatatcgatt cgcgagcggg cagttcgcag actcggcgaa ggaccgtttc
      361 gttggccgac gcggggggca ccgactacat tgtttccccg cacgacgagg tgcgcgtcgc
      421 caaggtattc accgatgtcg gcatcggctc ggtcaagctg caaggccaat ttcgtcatgc
      481 cggcacctat cagatcgttc gcggcgagcg tctctccgac gtgttgatgc gcgcgggcgg
      541 actcaccgac accgcctatc cttacggtac ggtgttgttg cgccgttccg cggcggaaaa
      601 ggaacgggaa gcgttccgac gcgaagctac cgagatcaac gatcagcttc tggccgccat
      661 gagcaggcgc gatccgatgg cgaagcttcc tccggaagcg ttcaccgcgc tgcaaagcta
      721 cgtcaacgat ctgaagaacc agccggcgct ggggcgggtg acgattacgg ccgatccaac
      781 cgtactcgcg gcaaatccat cgctggatcc gctgctcgag cccggcgacg tcgtattcgt
      841 tccgcagcgt ccctactcgg tttacgtgct gggacaagtc ctccagcccg gaaccgtgcc
      901 cttcagagcc aacatgagtg tctctgacta tatcgctcag gcgggcgggt acgcgcagta
      961 cgccgacaaa tcgcagactt tcgtggttct tcccgacggt tcggcacggc aagcggggac
     1021 ttcttggttc gatttcggcg gcaataaaat cccgccgggg agcacagtct tcgtttcccg
     1081 cgagattgcg ggcttcgatt ggcgccagac cattctcgac tccgcctcga ttttcagaga
     1141 tttcgcgacg actgcggcct ctctggcggt tctgtccaag cagtaacctc ttgatctcag
     1201 cgcgattcgc tggggtcgtg tccgacggtt gaatattcta gcgggcttgt gccaacccat
     1261 tgtgagactt gaataaaggg ggttttgccg gaggcctgga gaacgcatgg cccgcgtggc
     1321 cgtggcggtc ctgcgagccg cataatggag ccatagcgga ttgctattaa cttcattcag
     1381 acgcgatttt ttgcttggct tatcagagct ttgtcggtaa tataactatc gctagttgtt
     1441 gcacggacgc aacggtttct ggggggtggg tttgttgacc ataacggggt ccgtgtggta
     1501 actctcagat cacagtcggc agaggcatgg aggggattga tgtttaaaag atttggcgcc
     1561 gcggcgttgg cggcttcgtt gcttgtttca agcacggcgt ttgctgcggc caatgctaat
     1621 cagggtgcgc ttgcgcaagg taagcctgcc actgtgaagc aagcagagtc gtatcaaggc
     1681 cagaagtacg tttggtgggt tttgggggcg ggtgttgtga ttggcggtgt tgcgcttgtt
     1741 gcaagcggcg atggccatgg attggtcggt acaccatgcc cgtttggcgg ctgcccaac
//
LOCUS       DASWPU010000045         5354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_12613,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000045 DASWPU010000000
VERSION     DASWPU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12613"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGC27_00930"
     CDS             complement(<1..218)
                     /locus_tag="VGC27_00930"
                     /inference="COORDINATES: protein motif:HMM:NF044550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEY0281169.1"
                     /translation="MKTTYAPFDAADYLDNDEVIAEYLSAAIEDPKPDVFLAAIGDVA
                     KARGMAQIAKDAGLGRESLYKALSSGAHP"
     gene            complement(215..520)
                     /locus_tag="VGC27_00935"
     CDS             complement(215..520)
                     /locus_tag="VGC27_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0281170.1"
                     /translation="MNTFLRSPEFDAWLRAIADLKAKARVLARLRSATLGNFGDCEPV
                     GEGVSEMRIHAGPGYRVYFMRTGTTIYVLLVGGDKSTQKRDIARAKKMARELKETRT"
     gene            complement(574..2121)
                     /locus_tag="VGC27_00940"
     CDS             complement(574..2121)
                     /locus_tag="VGC27_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0281171.1"
                     /translation="MAGGRAASWPFAFLHGALAWIAALPSRNIRITVVLCVVAIGGSF
                     AAAGALQMRFDRVQAYRQAAYFEAKRANAVAAVTAASLARYERFGRLFASDQITDHTL
                     AASSELRGVAIADSAGQTLRASGEAIDMQSLTEPARKGRVVLDAGQRTLIAFADHNNT
                     VAVSFDSSALAPAAMMDGVELVNAAGLRLAGTPSDAEAVAVRVGNWPVEVRVALSDEN
                     ALRAWYGSLPLYLFVILGPALVGGGLAAVFVREFERRAKASEAIRALRSTHPAEARLL
                     VRLATAEREAVEAQRSKTEFVAHMSHELRTPLNAIIGFAEVIERGFYGAVGHPKYIEY
                     ARDIGMAGRSLHSKIGDILEFANLEAGRYPLKPIRFDVTELVCACVNENVGRAFSRRI
                     ALEMVPADAAEVNADPLAVKRIVTNLLSNALLYTQEGGRVRVSVREDEGAVAVAVTDN
                     GNGFNQDEASRAGTAFRRFERAGVSTGTGLGLAIVVALARRMGGAVRLASAHGEGTCA
                     ELRLPKA"
     gene            complement(2215..4845)
                     /gene="pepN"
                     /locus_tag="VGC27_00945"
     CDS             complement(2215..4845)
                     /gene="pepN"
                     /locus_tag="VGC27_00945"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY0281172.1"
                     /translation="MRTEEPRAVRLKDYRAPDYKVSEIALDFVLDPEATRVTATTKFA
                     RNGAPGGAAVPLVLDGEYLKLVSVAMDGKTLGAGDYAIGPETLTIAKPPERFTLEIVT
                     EIAPAKNTALEGLYLAKGIFCTQCEAEGFRRITYFLDRPDVLAVYTTRIEAPKAEYPV
                     LLSNGNRVAKGELNGGRHFAVWNDPFPKPCYLFALVAGDLGVLEDSFVTMSGRKVDLR
                     IFVEHGNEPKAAYAMDSLKRAMKWDEEKYGREYDLDIFMIVAVSAFNFGAMENKGLNI
                     FNDKVLLALPETATDDDYAHIEGVVAHEYFHNWTGDRITCRDWFQLSLKEGLTVFRDQ
                     SFSADQRSAPVQRIENVRALRLRQFQEDAGPLAHQVQPQSYITIDNFYTATVYEKGAE
                     IIGMLKTLVGEEGYRKATDLYFERHDGQAATVEDWVKCFEDTSGRDLGQFRLWYAQAG
                     TPEIEADGSYDAANKTYVLRLKQSLAPTPGQPVKQPMLIPVRLGLVGASGRALPLTLE
                     GENAPGPDERVLELTGAEQTFTFVGVDEEPLLSAGRGFSAPAVFKVAQSPQQRAALMA
                     RDADSFNRWEAGQAAARAAMLDLIAGKASKPDPHYVDAVGEVLKHAKDDMAFAANMLS
                     PPLESEMAMAMKTVDPDAIHAARATLIRTVAAAHGEAFAALYESLADAGAFSPDAASA
                     GRRALRNACLRYLTSADDDNAAALADMHYRSATNMTDMVAGLAALSRMVSPRTDAAFT
                     HFYDRFAKDPLVIDKWMGLQAGSPRPDTVLRVRALMSHPAYDIKNPNRVRALVGAFSA
                     NQLRFHAADGSGYALVGETIQSLDKLNAMVAARMAGAFETWRRFDSGRQALMRRELET
                     MVKTPGISSNLFEVTTKMLG"
     gene            complement(4903..>5354)
                     /locus_tag="VGC27_00950"
     CDS             complement(4903..>5354)
                     /locus_tag="VGC27_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY0281173.1"
                     /translation="ALPDGRIVMTDELWKMVQNDDEIEGVFAHEMANVDRAHGLQRVY
                     EASLVPAAIAVVTGDLSQVSQMSVILPGILVQSAYSREFERQADDDGAKLLLGMGGHP
                     ARMGDLLLRIDRKLCGKDGCGPGWLGSHPETSVRVERLRAADPARKK"
BASE COUNT          878 a         1721 c         1767 g          988 t
ORIGIN      
        1 gggtgcgcgc cggaactgag ggctttgtaa aggctttcgc ggcctagacc cgcgtccttg
       61 gcaatctgcg ccatgccgcg cgctttggcg acatcgccga tggccgcgag aaacacatcg
      121 ggcttggggt cttcgatggc cgccgaaagg tactcggcga tgacttcgtc gttgtcgaga
      181 taatccgccg catcgaaggg ggcgtaggtc gttttcatgt tcttgtctcc ttcaactcgc
      241 gagccatctt ttttgcgcgc gcaatatcac gcttctgtgt ggacttatcg ccgcccacga
      301 gcaggacgta gatcgtcgtc cccgtgcgca tgaaatagac ccgatagccc ggcccggcat
      361 gaatgcgcat ctcggagacg ccttcgccga ccggttcgca atcgccgaaa ttgccgagcg
      421 tcgcgctgcg caggcgcgct aaaactctgg ccttcgcttt gagatcggca attgccctta
      481 gccacgcatc gaattccggc gaccggagaa acgtgttcat gcattccgat tgtatccaat
      541 tggatacggt tgcgcaagtt caatttttgg aaattacgcc ttcggcaggc gcagttcggc
      601 gcaggtgcct tcgccgtggg cgctggcgag cctgaccgcg ccgcccatgc gccgcgccag
      661 cgccaccacg atggcgaggc ccagcccggt accggtcgag accccggcgc gctcgaagcg
      721 gcggaaggcc gtgccggccc gactggcttc gtcctggttg aagccgttgc cgttgtcggt
      781 caccgcgacg gcgaccgcgc cttcgtcctc gcgcacgctg acccgcacgc gcccgccttc
      841 ctgggtgtag agcagggcgt tcgacaacag gttggtgacg atgcgcttga ccgccagcgg
      901 atcggcgttc acctcggcgg cgtccgccgg caccatctca agggcgatgc ggcgcgagaa
      961 ggcgcggccg acattctcgt tgacgcaggc gcagaccagt tcggtgacat cgaagcggat
     1021 gggcttcagc ggatagcgcc cggcctccag attggcgaat tcgaggatgt cgccgatctt
     1081 ggaatgcagg ctgcgccccg ccatgccgat gtcgcgggcg tattcgatat atttgggatg
     1141 gccgaccgcg ccgtagaagc cgcgctcgat cacttcggcg aatccaatga tggcgttgag
     1201 cggcgtacgc aactcgtggc tcatatgagc gacgaattcg gtcttcgagc gctgggcctc
     1261 caccgcttcg cgctcggcgg tggccagccg caccagcagt cgcgcttcgg ccggatgggt
     1321 ggagcgcagc gcgcggatgg cttcggacgc cttggcgcgg cgttcgaact cgcgcacgaa
     1381 cacggccgcc agcccgccgc cgaccagcgc cggccccagg atcacgaaca gatagagcgg
     1441 cagcgagccg taccaggctc tgagcgcgtt ctcgtcactg agcgccacgc gcacttccac
     1501 cggccaattg ccgacccgca ccgcgacggc ctctgcgtca ctcggcgtac cggccagccg
     1561 aagcccggcc gcattcacca gctccacgcc gtccatcatg gccgccggcg ccagcgccga
     1621 gctgtcgaat gacaccgcca cggtattgtt atggtccgcg aaggcgatga gcgtgcgctg
     1681 ccccgcatcc agcacgacgc ggcctttgcg cgccggttcg gtcaggctct gcatgtcgat
     1741 ggcctcgccg ctggcgcgca gggtctgccc ggcggaatcc gcgatcgcta ccccgcgcaa
     1801 ttcggacgat gccgcgagcg tgtggtcggt gatctggtcc gatgcgaaca ggcggccgaa
     1861 ccgttcgtag cgcgccagcg aggccgccgt tacggcggcg acggcattgg cgcgcttggc
     1921 ttcgaaatag gcggcttggc gataggcctg aacgcggtcg aagcgcatct gcaatgcgcc
     1981 cgccgcggcg aagctgccgc cgatggcgac gacgcagaga acgaccgtga tgcggatatt
     2041 gcgcgacggc agagcggcaa tccacgccag cgctccatga aggaacgcga agggccatga
     2101 agctgctcgt ccgcccgcca tagtgaaaac accccagccc tgcgcggaca ggtgattcgc
     2161 gcttttgtcg attatgcgaa tcggtgagtc gctgtcacct gcttcggtac cgaatcaacc
     2221 cagcatttta gtcgtgacct cgaacaggtt cgaggaaatt cccggtgttt tgaccatggt
     2281 ttcgagttca cggcgcatca acgcctgacg ccctgagtcg aaacgccgcc aagtctcgaa
     2341 ggcgccggcc atgcgcgcgg cgaccatcgc gttgagcttg tcgagggact ggatggtctc
     2401 gccgaccagg gcgtagccgc tgccgtcggc ggcgtggaag cgcaactggt tggccgagaa
     2461 ggcgccgacc agcgcgcgca cgcgattggg attcttgatg tcgtaggcgg gatggctcat
     2521 cagcgcccgc acgcgcagca cggtgtcggg cctcggcgag cccgcctgca atcccatcca
     2581 tttgtcgatc accagcggat ccttggcgaa gcggtcgtag aagtgcgtga aggcggcgtc
     2641 ggtgcgcggc gacaccatgc gcgacagcgc ggcgaggccc gcgaccatat ccgtcatgtt
     2701 ggtcgccgaa cggtaatgca tgtcggcgag cgccgccgcg ttgtcgtcgt cggccgaggt
     2761 gagatagcgc aggcaggcgt tgcgcagcgc gcgccgcccg gccgaggcgg cgtccggcga
     2821 gaacgcgccg gcatcggcca ggctttcgta aagcgcggcg aaggcttcgc cgtgcgcggc
     2881 ggcgacggtg cggatcagcg tcgcgcgcgc cgcatggatg gcgtcgggat cgactgtctt
     2941 catcgccatg gccatttcgc tttccagcgg cggcgacaac atgttggcgg cgaaggccat
     3001 gtcgtccttg gcatgcttca gcacttcgcc gacggcatcg acataatgcg gatcgggctt
     3061 ggacgctttg ccggcgatca ggtcgagcat ggccgctctg gccgccgctt ggcccgcttc
     3121 ccagcggttg aaggaatcgg catcgcgcgc catcagcgcc gcgcgctgct gcgggctctg
     3181 cgccaccttg aagaccgccg gcgcggaaaa gccccgcccg gccgacagca gcggttcctc
     3241 gtccacgccg acgaaggtga aggtctgttc cgccccggtc agttcgagca cccgctcgtc
     3301 cggccccggc gcgttctcgc cttccagcgt cagcggcaag gcgcggccgg atgcgcccac
     3361 cagccccagc cgcaccggaa tgagcatcgg ctgcttgacc ggctgtcccg gggtgggtgc
     3421 aaggctctgc ttcaaacgca ggacgtaggt tttgttcgcg gcgtcgtagc tgccgtccgc
     3481 ttcgatctcc ggtgtgccgg cctgcgcata ccacaggcgg aattgcccga gatcgcgccc
     3541 gctggtgtcc tcgaagcatt tgacccaatc ttccaccgtc gccgcctggc cgtcgtggcg
     3601 ttcgaaatag agatcggttg ccttgcgata gccttcctcg cccaccaggg tcttcagcat
     3661 gccgatgatc tcggcgcctt tctcgtaaac cgtcgcggta tagaaattgt cgatggtgat
     3721 gtagctctgc ggctgcacct gatgggcgag cgggccggcg tcttcttgga actggcgcag
     3781 gcgcagcgcg cgtacgtttt cgatgcgctg gacgggggcc gagcgctgat cggcggaaaa
     3841 gctctggtcg cggaacacgg tcaggccttc cttgagcgaa agctggaacc agtcgcggca
     3901 ggtgatgcgg tcgcccgtcc agttgtggaa atattcgtgg gcgaccacgc cttcgatgtg
     3961 ggcgtaatcg tcgtcggtgg cggtttcggg caaagccaga agcaccttgt cgttgaagat
     4021 gttgagaccc ttgttttcca tcgccccgaa gttgaaggcc gacacggcga cgatcatgaa
     4081 gatatcgaga tcgtattcgc ggccgtattt ttcctcgtcc catttcatcg cccgcttgag
     4141 cgaatccatg gcgtaagcgg ctttcggttc gttgccgtgc tcgacgaaga tgcgcagatc
     4201 gaccttgcgc ccgctcatgg tgacgaagct gtcttccagc acgcccagat cgccggccac
     4261 cagggcgaac aggtagcagg gcttgggaaa cggatcgttc cataccgcga agtgcctgcc
     4321 gccgttgagt tcgcccttgg cgaccctatt gccgttcgac agcagcaccg gatattcggc
     4381 cttcggggcc tcgatgcggg tggtatagac cgccagcacg tcgggccggt cgaggaaata
     4441 ggtgatgcgc cggaaccctt ccgcttcgca ttgggtgcag aagatgccct tcgccagata
     4501 aagcccttcg agcgccgtgt tcttggcggg cgcgatctcg gtcacgattt cgagcgtgaa
     4561 gcgctccggc ggcttggcga tggtgagcgt ctccgggccg atggcatagt ccccggcacc
     4621 cagcgttttt ccgtccatag cgacggacac cagcttcaga tattcgccgt cgagcaccag
     4681 cggcacggcg gcgcccccgg gcgcgccgtt gcgtgcgaat ttcgtggtgg cggtgacgcg
     4741 cgtggcttcg ggatcgagca cgaaatcgag cgcgatctcc gacactttat aatccggcgc
     4801 gcgataatcc ttcagacgga cggcacgggg ctcttcggtg cgcatgcagg gtccttgctg
     4861 gtgggccaga tgggtatagc cgaaagcggt acgaagttgc tgctattttt tccgggcggg
     4921 gtctgcggcg cgcaggcgct cgacgcgcac cgaggtttcc ggatggctgc cgagccagcc
     4981 cggcccgcag ccgtccttgc cgcagagttt ccggtcgatg cgcaacagca agtcgcccat
     5041 gcgcgcgggg tgaccgccca tgccgagcag cagcttggcg ccgtcgtcgt cggcctgccg
     5101 ttcgaattcg cgcgaatagg ccgactgcac caggatgccg ggaaggatga ccgacatctg
     5161 gctcacttgc gaaagatcgc cggtgacgac ggcaatggcg gcgggcacca gcgaggcttc
     5221 gtagacgcgc tgcaagccgt gggcgcggtc cacattggcc atttcgtgcg cgaagacgcc
     5281 ttcgatctcg tcgtcgttct gcaccatctt ccacaattcg tccgtcatca cgattcgtcc
     5341 gtcgggcagg gcga
//
LOCUS       DASWPU010000046         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_98167,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000046 DASWPU010000000
VERSION     DASWPU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98167"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VGC27_00955"
     CDS             complement(<1..321)
                     /locus_tag="VGC27_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281174.1"
                     /translation="MSPFLERVNRTKVTADLLGDVLKMHAAMAPEDRAVLEVRFIRLL
                     ELLDKKGLGRGRRRAALATAIEFRLEALAGLESDHALRAWIMPGEELGCDYVHAELLE
                     AAAKE"
     gene            598..831
                     /locus_tag="VGC27_00960"
     CDS             598..831
                     /locus_tag="VGC27_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281175.1"
                     /translation="MTEEELEKAVANLSSGEQTKFQAWFCDFITARFGEDAYVTTPSE
                     LEGIKRGLHDAAEGKFATDEQVEAVFAKHRPKS"
     gene            complement(828..1157)
                     /locus_tag="VGC27_00965"
     CDS             complement(828..1157)
                     /locus_tag="VGC27_00965"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEY0281176.1"
                     /translation="MKFVLDAQLPPRLAKALVRAGHVAWHVYECGLLTAKDRDIWRFA
                     MQEGAAVVTKDADFPAMRMHAPDGPPVVWLRLGNTGNDALEKALLSALPEIVAAIAAG
                     EGMVELA"
     gene            complement(1154..1561)
                     /locus_tag="VGC27_00970"
     CDS             complement(1154..1561)
                     /locus_tag="VGC27_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY0281177.1"
                     /translation="MAVVKIHDAKTNLSKLIARVEAGEEIIIARDDKPVAKLTAMREA
                     DGARLSYEFAEEAAPYEHLPLRARITTDPEVCGGKPCIRGMRIRVSDVVGLLAGGMSR
                     ENILTDYPYLEAADIDAALAYAAGSANHRIIRA"
     gene            complement(1630..>1927)
                     /locus_tag="VGC27_00975"
     CDS             complement(1630..>1927)
                     /locus_tag="VGC27_00975"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0281178.1"
                     /translation="REILEEAGYEVEKLERISSFYSTPGITSERVILFFAETRGDRPV
                     AKGGGLTEENEDIKVLELPPEEAFRQVERGEIADAKTLIGLMWLKERMAQGRVE"
BASE COUNT          364 a          620 c          533 g          410 t
ORIGIN      
        1 ttccttggcc gctgcctcga gaagttcagc gtgcacataa tcgcagccca gttcctctcc
       61 gggcatgatc catgcccgca acgcatgatc agactccaac cctgcgagtg cctcgaggcg
      121 gaattcaatt gctgtagcga gcgctgcccg tcttcttccc ctgcccaagc ctttcttgtc
      181 gagcaattcc aataaccgaa tgaaccgcac ttcgagaacg gctctgtctt caggcgccat
      241 tgctgcgtgc atcttcagca cgtcgccgag taagtcagcg gttacctttg tgcgatttac
      301 tcgctccaaa aagggtgaca tcgacgatcc cttgcatcaa gttccagacg ctctgttgta
      361 ccacaacggt cgtgcgcagt tgtagaatgc acgagtcgtt ttctgtggaa ttagtgctcc
      421 tactatcgcg ggaatgagac tttccagatc gcttacaccc agtttcgtca tggccgggct
      481 cgacccggcc atccagcggg cgagcgtccg tgagccgaaa actctggaag gccgcctcaa
      541 gggcggccat tacgaaaaag ggcaaatatt gtcttctgat atgttaggat tggcctcatg
      601 actgaggaag aactggaaaa agccgtcgca aatctttctt ccggcgagca aaccaagttt
      661 caagcttggt tctgtgattt catcactgct cgcttcggcg aagacgctta tgtcacgacg
      721 ccatcggaac ttgaaggcat aaaacgcggc ttgcacgatg cggcggaggg caaattcgcg
      781 actgacgagc aagtggaagc cgtcttcgcg aagcatcgtc ccaagtctta ggccaactcc
      841 accatcccct cccccgccgc aatcgccgca acgatttccg gcagcgccga cagaagcgct
      901 ttttccagcg catcgtttcc ggtattgccg agccgcagcc agacgaccgg cgggccgtcg
      961 ggcgcgtgca tccgcatcgc ggggaaatcg gcgtccttgg tgacgaccgc cgcgccctcc
     1021 tgcatcgcaa accgccagat gtcgcgatcc ttcgccgtta acagcccgca ttcgtagacg
     1081 tgccatgcca cgtggccggc gcgcaccagc gctttggcca gccgcggcgg cagttgcgca
     1141 tcgaggacga acttcacgcg cggatgatgc ggtggttcgc cgatccggcg gcataggcga
     1201 gcgccgcgtc gatgtccgcc gcctcaaggt agggatagtc ggtaagaatg ttctcgcggc
     1261 tcatgccgcc cgccaagagg ccgaccacgt cgctcacgcg gatgcgcatg ccgcggatac
     1321 atggtttgcc gccgcacact tccggatcgg tggtgatgcg cgcgcgcagc ggcaggtgct
     1381 catagggcgc ggcctcttcg gcgaattcgt aggacaaccg cgcgccgtcc gcctcgcgca
     1441 tcgccgtcag tttcgccacc ggcttgtcgt cgcgggcaat gatgatctcc tcgcccgcct
     1501 caacgcgcgc gatgagcttc gacaggttgg tcttggcgtc gtgaatcttg acgactgcca
     1561 tagcgtggct ccttagctaa taagttagct aacatgcgcg gcctcgtcaa cttcattttg
     1621 gcccacgacc tattctaccc gtccctgcgc cattctctcc ttcaaccaca tcaggccgat
     1681 gagggtcttg gcgtcggcga tctcgccgcg ttcaacttgc cggaacgcct cttcgggcgg
     1741 caattcgagc accttgatgt cttcgttctc ttcggtcagt ccgccgccct tggccaccgg
     1801 ccggtcgccg cgcgtttccg caaagaacag aataacccgc tccgaggtga tgcccggcgt
     1861 cgaatagaag ctggaaatgc gctccagctt ttccacttcg tacccggctt cctcaagaat
     1921 ttcccgg
//
LOCUS       DASWPU010000047         4325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_73782,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000047 DASWPU010000000
VERSION     DASWPU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73782"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /gene="radA"
                     /locus_tag="VGC27_00980"
     CDS             <1..1065
                     /gene="radA"
                     /locus_tag="VGC27_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY0281179.1"
                     /translation="GSAVYISGEEAMAQVRLRAGRMDLSDAPVALGSATCIEDILTTL
                     EKGKTPGVVAIDSIQTIWTGALEAAPGTIAQVRTASFDLVRYAKSTGAAVILVGHVTK
                     DGQIAGPKAVEHLVDAVLYFEGERGHQFRILRAVKNRFGPTDEIGVFEMTGKGLAEVA
                     NPSALFLGDRASTSPGTAVFAGIEGTRPLLVEIQALVAEAGYGSPRRAVVGWDSGRLA
                     MILAVLDARGGLGVSAHDVYLNVAGGLKVGEPAADLAAAAALISSFSGQALPRDTVFF
                     GEISLSGDVRPAPQAELRLKEAAKLGFSTAVVPRETRTDTAGLSLRPIKDVGDLLALA
                     GAAGPARPARAATRSGKIES"
     gene            1062..1745
                     /locus_tag="VGC27_00985"
     CDS             1062..1745
                     /locus_tag="VGC27_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY0281180.1"
                     /translation="MSVAFQFIDVAVVVIIIASTVYATYRGFISETLSIFAWIAAAFA
                     TLYFGSWLAHLTRDMISPPWLGLLVGYAAVFLVVLIPLSFMSYRFSEGVKKSQVSAID
                     RALGGAFGVVRALAIIGIAYLVFSAIVPIPTQPGWIANARLLPLIQGSAEVVASLVPD
                     QDLDRGPRAREKPREKPVIHKESAKPAPAPAAKPAAKKTQKKTYGAKDRQALDRLIET
                     TGSNGNGKP"
     gene            1742..3211
                     /gene="purF"
                     /locus_tag="VGC27_00990"
     CDS             1742..3211
                     /gene="purF"
                     /locus_tag="VGC27_00990"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY0281181.1"
                     /translation="MTGLTGDFGFEDDTLHEECGVFGIFGHPGAGALTVLGLHALQHR
                     GQEAAGIVTFDKGQFVAERHPGLVGDIFGKNNSYAEKLKGSFAIGHNRYSTAGGSAVR
                     NIQPLFADLTGGGIAIAHNGNLTNARLLRARLVQEGAIFQSTSDTEVIIHLTARSSFS
                     PIVDKLIDALKQVEGAYSLVALTSKKLIGVRDPWGVRPLVLGRFEGAPILASESCALD
                     IIGAEFERDVKPGEVIVITKDGIESHYPFLKQRPRFCVFEYIYFARPDSTVEGMNVYE
                     ARKRIGEELAKEAPVAADMVIPVPDSGTPAALGYAAASGLPFDLGIIRNHYVGRTFIE
                     PSDGIRHFSVRRKHNPNRAILAGKRVIMIDDSIVRGTTSKKIVQMVRDAGASEVHFRV
                     ASPPTRHSCFYGVDTPNEHALLAHEMKIEEMRSFIGADSLAFVSIDGLYRAMGEAAGR
                     DPAAPRFCDACFSGDYPIPLTDTADGAKNGQLSFLAEVA"
     gene            3307..4023
                     /locus_tag="VGC27_00995"
     CDS             3307..4023
                     /locus_tag="VGC27_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0281182.1"
                     /translation="MTRSLANRIALVTGASRGIGRAAAKALAASGAHVILVARTVGGL
                     EEADDEIRQAGGSATLVPLNLKDFDALDRLGASLFERWGRLDAFFANAGVLGAVSPLT
                     HLEPKTVEELLDVNVTANWRLIRSLDPLLRRSEAGRVLFVSSGVARKHIPYWGGYAMS
                     KAALESLALTYAAECEGTNIKVNLLNPGPVRTGMRAKAMPGEDPETLPPPEALTPLIV
                     DMLSPSYEKNGELAVFKRTA"
     gene            complement(4020..>4325)
                     /locus_tag="VGC27_01000"
     CDS             complement(4020..>4325)
                     /locus_tag="VGC27_01000"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281183.1"
                     /translation="PAVLAADRAWNTRIPTATLNRFLEDALTRHAPPAIHGRRVRIRY
                     MTQPKSRPPTFALFGTQLKALPEAYLRYLANNLRETFHLEGAPIRFLLRTSKNPYAD"
BASE COUNT          759 a         1386 c         1377 g          803 t
ORIGIN      
        1 ggcagcgcgg tctatatctc cggcgaagag gcaatggcac aggtgcgcct gcgcgccggc
       61 cgcatggacc tttccgatgc gccggtggcg ctcggctcgg ccacctgcat cgaagacatt
      121 ctcaccaccc tggaaaaggg caagacgccg ggcgtggtcg ccatcgattc catccagacc
      181 atctggacgg gtgcgctgga agcggcgccg ggcacgatcg cacaggtgcg cacggcctcg
      241 ttcgatctgg tgcgctacgc caaatcgacc ggcgcggcgg tgatccttgt cggccatgtc
      301 accaaagatg gccagatcgc cggtcccaag gcggtggagc atctggtcga tgccgtcctc
      361 tatttcgaag gcgagcgcgg tcaccagttc cgcattctgc gcgcggtgaa gaaccgcttc
      421 gggccgaccg acgagatcgg cgtcttcgag atgaccggca agggcttggc ggaagtcgcc
      481 aatccttcgg cgctgttcct aggcgaccgc gccagcacct cgccagggac ggcggtcttc
      541 gcgggcatcg aaggcacgcg ccccttgctg gtcgaaatcc aagctttggt cgcggaggcc
      601 ggctacggct cgccgcgccg cgccgtcgtc ggctgggatt ccggccgtct ggcgatgatt
      661 ctcgccgtgc tcgacgcccg cggcggactg ggagtcagcg cccacgatgt ctatctcaat
      721 gtggcgggcg gcttgaaagt cggcgaaccg gcggcggatc tcgccgccgc ggcagccctg
      781 atttcgtcgt tcagcggtca agctttgccg cgcgacacgg tgtttttcgg cgagatcagc
      841 ctttcgggcg atgtgcgccc cgcgccgcag gccgaattgc gcttgaaaga ggccgcaaaa
      901 ctgggttttt ccaccgctgt ggtgccgcgg gaaacccgta ccgacaccgc cggactgtct
      961 ttgcggccta ttaaggatgt cggcgatcta ctggcgcttg ccggggccgc gggcccagcg
     1021 cgccccgcgc gcgcggcaac gagaagtggt aaaatagaat catgagcgtg gcgttccagt
     1081 tcatcgatgt ggcggtggtg gtgatcatca ttgcgtctac cgtctacgcg acgtatcgcg
     1141 gcttcatctc ggagacgtta tcgattttcg cctggatcgc ggccgccttt gcgacgctct
     1201 attttggttc ctggctggcg cacctgacgc gcgacatgat ttcgcccccc tggctgggct
     1261 tgctggtggg gtacgccgcg gtgttcctgg tcgtcctcat cccgctgtcg ttcatgagtt
     1321 accgcttttc ggaaggggtg aagaaatcgc aggtcagcgc gatagaccgc gcgcttggcg
     1381 gggctttcgg cgtggtgcgc gcgctggcga tcataggcat cgcctatctg gtcttttcgg
     1441 ccatcgtacc catcccgacc cagcccggct ggatcgccaa tgcccgcctt ctgccgctga
     1501 tccaaggatc ggccgaggtc gtcgcttccc ttgtcccgga tcaggatttg gatcgcggtc
     1561 cccgtgcgcg ggaaaaaccc cgcgaaaaac cggttatcca taaggaatca gcaaaacccg
     1621 cgccggcccc tgcggcaaaa ccggctgcca aaaaaacaca gaagaagacc tatggcgcca
     1681 aggaccgaca agcgctagac aggctcatcg aaacaacagg cagcaacgga aacggcaagc
     1741 catgaccggc ctgaccggcg atttcggctt tgaagacgat acgctgcacg aagagtgcgg
     1801 cgtattcggc attttcggcc atcccggtgc cggcgccttg accgtgcttg gcttgcacgc
     1861 gctccagcac cgcggccagg aagccgccgg aatcgtgacc ttcgacaagg gccagttcgt
     1921 ggccgagcgc catccgggct tggtcggcga cattttcggc aaaaacaatt cgtatgccga
     1981 aaagctgaaa ggttctttcg ccatcggcca caaccgctat tcgacggcgg gcggatcggc
     2041 ggtgcgcaac atccagccct tgttcgccga tctgaccggc ggcggcattg ccatcgccca
     2101 taacggcaat ctcaccaatg cgcgcctgct gcgcgcccgc ctggtgcagg aaggcgcgat
     2161 tttccagtcc acctccgaca ccgaagtcat catccacctg accgcgcgca gcagcttcag
     2221 cccgatcgtc gacaagctga tcgacgcgct caagcaagtg gaaggcgctt attccctggt
     2281 ggcgctgacc tccaagaaat tgatcggcgt acgcgatcct tggggcgtgc gcccgctggt
     2341 gctggggcgt ttcgagggcg cgccgatcct ggcgtcggaa agctgcgctc tcgacatcat
     2401 cggcgcggag ttcgaacgcg acgtcaagcc gggcgaagtg atcgtcatca ccaaggacgg
     2461 catcgaatcc cattacccct tcctcaagca gcgcccgcgc ttctgcgttt tcgagtacat
     2521 ctatttcgcg cgcccggatt cgaccgtgga gggcatgaat gtctacgagg cgcgcaaacg
     2581 catcggcgag gaacttgcca aggaggcgcc cgtcgccgcc gacatggtga tcccggttcc
     2641 cgactccggc acgccggccg cgctcgggta cgccgcggcg tccggactgc cgttcgatct
     2701 tggcatcatc cgcaaccatt atgtcgggcg caccttcatc gaaccgtccg acggcatccg
     2761 ccatttcagc gtgcgccgca aacacaatcc caatcgcgcc atactcgccg gcaaacgcgt
     2821 catcatgatc gacgattcca tcgtccgcgg gacgacctcc aagaagatcg tgcagatggt
     2881 ccgcgacgcg ggagcctcgg aagtccattt ccgcgtcgcc agtccgccga caaggcattc
     2941 ctgcttctac ggcgtcgata cccccaacga acacgccttg ctcgcccacg agatgaagat
     3001 cgaggagatg cgcagtttca tcggcgcgga cagcttggcg ttcgtctcca tcgacgggct
     3061 ttaccgggcg atgggcgagg cggcaggccg cgatccggcc gcgccgcgct tctgcgacgc
     3121 ctgcttctcc ggcgattatc cgattccgct gaccgacacg gccgacggcg ctaagaacgg
     3181 ccagctttcg ttccttgccg aagtggcgtg aataagggcg agtagcgaat agggagtagc
     3241 gaataggggc tttctattcc attcgctatt cgctattcgc tattcgccgt cacatcgcgc
     3301 tcgaacatga cccgctccct tgccaaccgc attgctcttg tcacgggagc ttcgcgcggc
     3361 atcggacgcg ccgcggccaa agcgctggct gcttccggcg cgcatgtgat cctggtggcg
     3421 cgcaccgtcg gcgggctgga agaagccgac gacgaaatcc gccaagccgg cggcagcgcc
     3481 acgctggttc ccttgaacct caaggatttc gacgcgctgg atcgccttgg tgcatcgctc
     3541 ttcgagcgct ggggcaggct cgacgcgttc ttcgccaatg ccggcgtgct gggcgcagtg
     3601 tccccgctca cccatctcga gcccaagacc gtcgaagaac tgctggatgt caatgtgacc
     3661 gccaattggc ggctgatccg ctcgctcgat ccgctgctgc gccggtccga ggcgggcagg
     3721 gtgctgttcg tcagttccgg tgtggcgcgc aagcacattc cgtactgggg tgggtatgcg
     3781 atgtcgaagg cggcgctgga atcgctggcg ctgacctatg cggcggaatg cgaaggcacc
     3841 aacatcaagg tgaacctgct caatccgggg ccggtgcgca ccggcatgcg cgccaaggcg
     3901 atgcccggcg aagaccctga gaccttgccg ccgcccgagg cactgacgcc gttgatcgtg
     3961 gacatgctgt cgccgtccta tgaaaagaac ggcgagctgg ccgtgttcaa acgtaccgcc
     4021 taatccgcat acggattttt tgaggttcgc agcagaaagc ggatcggggc gccttcaagg
     4081 tggaaggtct cgcgcagatt gttggccaga taacgcagat aagcctctgg cagcgccttc
     4141 aactgggtgc cgaacagcgc gaaggtcggc ggccggctct tgggctgagt catgtagcgg
     4201 atacgcacgc gccggccgtg gatggcgggc ggggcatgcc gcgtcagtgc gtcttcaaga
     4261 aagcgattga gggttgcggt ggggatgcgc gtgttccagg cgcggtcggc cgccagcacc
     4321 gccgg
//
LOCUS       DASWPU010000048         4110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_12627,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000048 DASWPU010000000
VERSION     DASWPU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12627"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..606
                     /gene="arsC"
                     /locus_tag="VGC27_01005"
     CDS             238..606
                     /gene="arsC"
                     /locus_tag="VGC27_01005"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523256.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEY0281184.1"
                     /translation="MHITIYHNPSCGTSRNVLAAIRAAGQEPRIIEYLKTPPTREELK
                     HLIARMNLTVRDVVRKKEPLYAELRLDERDVGEDELLDAMIENPILINRPIVVTERAV
                     RLCRPSELVKDLLGSEASQP"
     gene            603..785
                     /locus_tag="VGC27_01010"
     CDS             603..785
                     /locus_tag="VGC27_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281185.1"
                     /translation="MSGSATFKLSRVFAEGWNAASRLTAEESDGLDQRGAALNPYANE
                     PEKSRWSEGFAKALNK"
     gene            1002..2249
                     /locus_tag="VGC27_01015"
     CDS             1002..2249
                     /locus_tag="VGC27_01015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0281186.1"
                     /translation="MTNPSFTELGVAEPLRRALVAEGYTEPTPIQVSAIPVLMAGRDL
                     LGLAQTGTGKTAAFALPLLQQLSVGHEQRRGRSVRALILAPTRELAIQIHDSLRTYGR
                     FLHLRTAVIVGGVSQGAQVKAIAGGLDILVATPGRLLDLMEQGHVRLDAVTHLVLDEA
                     DRMLDMGFIRDIRKIMAALPKKRHSMLFSATMPADVEHLAQSILHQPVRIDVSPPKRT
                     AENIDQRVYYVAAAEKRALLATLLKDAALTRVLVFTRTKHTANRVADHLDKNGFPADA
                     IHGNKSQGARQRALERFRSGDSRILVATDIAARGIDIDNITHVINFELPNEPENYVHR
                     IGRTARAGAAGIAIAFCDPAENGYLRDIERLTKASLTVVQGERGADQPAKARDYARKT
                     PFRGKREKYGNRDRHNGGARRVA"
     gene            2494..2697
                     /locus_tag="VGC27_01020"
     CDS             2494..2697
                     /locus_tag="VGC27_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0281187.1"
                     /translation="MAIGTVKFFNSSKGFGFISPEGGGKDVFVHATAVEMAGMRTLAE
                     GQRVSFDIQPDAKGSKAVNLQAV"
     gene            2806..3201
                     /locus_tag="VGC27_01025"
     CDS             2806..3201
                     /locus_tag="VGC27_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281188.1"
                     /translation="MSYQQRRERIEPEQETDTTARKLRADQPILSELIKVLTAHPGGL
                     RRWSVMRAIRNDRERASREIPQKFEDEVERTFRRFCADADGAKTRICDPTEALFYRPR
                     EKAGEVWAIFPERASAWLNTETDKAEDGR"
     gene            3224..3466
                     /locus_tag="VGC27_01030"
     CDS             3224..3466
                     /locus_tag="VGC27_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281189.1"
                     /translation="MAADRPISEHEGALFDAVRVLATAVLDLGADAGVLRRRLTEARD
                     AAEALGNKHGAETLDFLIGSLFGVPDPSPKPSFRIV"
     gene            3649..>4110
                     /locus_tag="VGC27_01035"
     CDS             3649..>4110
                     /locus_tag="VGC27_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281190.1"
                     /translation="MLDDMIDYIAQVRERPVWQPIPQESRDRFRAGLPRRPTELREAY
                     DEFTRHVAPYTSGNVHPRFMGWVHGGGTAVGMLAEMLAAGLNANLGGRDQMPIEVERQ
                     IVEWARSMFGFPAGASGIERQSRRPRSDADRSRTPDRRMGAFDVRLSRGGQR"
BASE COUNT          809 a         1309 c         1294 g          698 t
ORIGIN      
        1 ggtacggcga tgggcacggt gaagagccgc gtcggtcgcg cgcgcgccgc gctgatgaag
       61 atgcttgacg gcgacgcacc tctgccgccg cgcatggccg cacattctac cggcgcctcg
      121 gacgccattc tggcgcagct cggcgcactc atgcctgccg gcgccgccta tgcgtgagcc
      181 gagccggcca aacggtgctg gagcggcggg caggggagac aatatcagga gcctaccatg
      241 catattacca tctatcacaa tccaagctgc ggaacctcgc gcaacgtcct tgccgccatc
      301 cgcgccgccg gccaggagcc gcgtatcatc gaatacctga aaacaccccc gacgcgcgag
      361 gaattgaagc atctgattgc gcgcatgaac ctgacggttc gagacgtcgt aaggaagaag
      421 gagccgttgt acgccgagct tcgccttgac gagagggatg tcggcgaaga cgaactcctc
      481 gacgcgatga tcgagaaccc gatcctgata aaccggccga tcgtggttac ggaacgggcg
      541 gtgcggctat gccgcccgag cgagttggtg aaggatttgc ttggcagcga ggcgtctcaa
      601 ccatgagcgg cagcgcaacc ttcaaactga gccgcgtctt tgccgaaggc tggaacgcgg
      661 caagcaggct gactgccgaa gaaagcgacg gcttggatca gcgcggcgcg gcacttaatc
      721 cctatgcgaa cgaacccgaa aaatctcggt ggagcgaagg tttcgccaag gcgttaaata
      781 aatagccgga cgggcctcat gggctgttct cttgacattt cggtaaggac accgctatag
      841 gctgccaacc tcgcgatttg cacgacgcgc acccggtgcc tgtcatcgcg aggatcatcg
      901 aaacaatccg ttttttccga acgtgcatgg gaagcggcaa aacgccgtac ccgcgcgtcc
      961 taaacggccg ctcgttttct tggcgtagtt aggacaattc agtgacaaat ccatctttta
     1021 ccgagcttgg cgttgccgag ccgcttcggc gcgcccttgt ggccgaaggc tataccgaac
     1081 cgacgcccat ccaggtcagc gccattccgg tgctcatggc ggggcgggat ctgctcggtc
     1141 tggcgcagac cggaaccggc aagaccgccg ccttcgcgct gccgctgttg cagcaacttt
     1201 cggtcggaca cgaacagcgc cgaggacgtt ccgtgcgcgc cttgatcctg gcgccgacgc
     1261 gggaactggc gatccagatc cacgacagcc tgcgcaccta tgggcgcttt ctgcatcttc
     1321 gcaccgccgt tatcgtcggc ggcgtgagcc agggcgcgca ggtcaaggcg atcgccggcg
     1381 gcctcgacat tttggtggcg acgccggggc ggctgctcga cctcatggag cagggccatg
     1441 tgcggctcga tgccgtgacg caccttgtgc tcgacgaagc cgaccggatg ctcgacatgg
     1501 gcttcatccg cgatatccgc aagatcatgg cggcgctgcc caagaagagg cattccatgt
     1561 tgttttcggc gaccatgccg gcggacgtgg agcatctggc gcagtcgatc ctccaccagc
     1621 cggtgcggat cgacgtttcg ccgcccaaac gcaccgccga gaatatcgac cagcgcgtct
     1681 attatgtcgc cgcggccgag aagcgcgcgc tgctcgccac gctgctcaaa gatgccgcgc
     1741 tgacgcgcgt tctcgtcttc acgcgcacca agcacacggc aaaccgcgtg gccgaccatc
     1801 tcgacaagaa cggctttccg gccgacgcca tccacggcaa caagtcccag ggcgcgcgcc
     1861 agcgggcgct ggaacggttc cgttcgggcg attcccgcat tctggtcgcg accgatatcg
     1921 cggcgcgcgg catcgacatc gacaacatca cgcacgtcat caatttcgag ctgccgaacg
     1981 agccggagaa ctatgtgcac cgtatcggcc ggacggcgcg cgccggcgcc gcgggcatcg
     2041 caatcgcgtt ctgcgatccc gccgagaacg gctatcttcg ggacatcgag cggctgacca
     2101 aagcgtcgct caccgtcgtg caaggcgagc gcggcgccga tcaaccggcc aaagcccgcg
     2161 attacgcccg caagacgccg tttcgcggca agcgcgagaa atacgggaat cgcgaccggc
     2221 ataatggcgg cgcgcgtcgc gtggcctgaa agacgtacgg gaagggcatg aaatgaaagg
     2281 caattgctac ctttcccgag cgcaacttgc aattggcgca caaagcatct attaaagacg
     2341 cagcgacggc gttccgtcag atgaagcgca ctgttgtact cccgactcgc attgtgcgcg
     2401 cctggccgaa acagccgaac ttccagcctg attgtctccg ctcgcgccgc ccaagcggtt
     2461 gtttgggcgc aacccgatgc actaggagta aacatggcta tcggaacagt gaagtttttc
     2521 aattcgtcca agggctttgg ctttatttcc ccggaaggcg gcggcaagga cgtgttcgtt
     2581 catgcgacgg ccgttgaaat ggcgggcatg cgcacgttgg ccgaaggcca gcgcgtatcg
     2641 ttcgacattc agccggacgc caaaggctcg aaggcggtca atctgcaggc cgtttaaggg
     2701 tcgtttataa gacgggaacg gcggaggtac gcctccgccg catcccgccc cgactatcgc
     2761 gaacgcgcgc tcgagccgcc gccgcggttg ctgcaaggaa gtcgaatgtc gtatcaacaa
     2821 cgtcgcgaac gcatcgagcc ggagcaagaa accgatacca ccgcacgcaa acttcgcgcc
     2881 gatcaaccta ttctgtccga acttatcaag gtcctcaccg cgcacccggg cgggctgcgg
     2941 cgctggagcg tgatgcgggc gatccgcaac gaccgcgaac gcgcgtcgcg cgaaatcccg
     3001 caaaagttcg aagacgaagt cgaacgcact ttccgcaggt tctgcgccga tgcggacggg
     3061 gcgaagaccc gcatatgcga tccgaccgag gcgctgttct atcggccccg cgaaaaggcc
     3121 ggcgaggtat gggcgatctt tcccgagcgc gccagcgctt ggctcaatac cgaaacggac
     3181 aaggccgaag acggacgtta gtcggccgca gcggagtcac gacatggcag cggatagacc
     3241 gatctccgaa cacgaaggcg cgctgttcga tgccgttcgc gtgctcgcga ccgccgttct
     3301 tgatctcggc gcggatgccg gcgttctgcg ccggcgtctg accgaagcgc gcgacgcggc
     3361 agaggcgctg ggaaacaaac atggcgccga gacgctggat tttctcatcg gctcgctctt
     3421 cggcgtaccc gatcccagcc ccaagccgtc gttccggatc gtctgatccg aatgcgcgcc
     3481 ggcgcgccgg gctgcattgc acaagcattt cgacgccttc gctgccgtgc tgtataagca
     3541 cgccggaaac cttcgagtcc gccccgcgtg agcaccagga acagcgattt atccggcatc
     3601 actctcgatc ccgaagactg ggccgagctg cgggccctgg gccacgaaat gctcgacgac
     3661 atgatcgatt acatcgcgca ggtccgcgag cgtccggtgt ggcagccgat tccgcaagag
     3721 tcgcgcgatc gcttccgggc cgggctgccg cgccgcccga ccgagcttcg cgaggcctat
     3781 gacgaattca cccgtcatgt ggcgccctat accagcggaa acgtccatcc ccgcttcatg
     3841 ggctgggttc acggcggcgg cacggcggtc gggatgctgg cagaaatgct ggcggcggga
     3901 ttgaacgcca atctcggcgg ccgcgatcag atgccgatag aagtcgaacg ccagatcgtc
     3961 gaatgggcgc gttcgatgtt cggctttccc gcgggggcca gcgggattga acgccaatct
     4021 cggcggccgc gatcagatgc cgatagaagt cgaacgccag atcgtcgaat gggcgcgttc
     4081 gatgttcggc tttcccgcgg gggccagcgg
//
LOCUS       DASWPU010000049         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_24787,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000049 DASWPU010000000
VERSION     DASWPU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24787"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1640
                     /locus_tag="VGC27_01040"
     CDS             <1..>1640
                     /locus_tag="VGC27_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY0281191.1"
                     /translation="LSQHAGQNYHQEWIGWAMPVVLLATGLTLAVLAADLRLKPLLKG
                     AATTAALFYWLGSLLKLPAFFTRKPYDDDSDIEPDVEDEGDFGLGIAPEPLASSSREN
                     RVRREEARKAPLKRARQPALSLESSEYQLPALGLLAEPKHIADATALSDESLEENARM
                     LEAVLADFGVKGRIVAVRPGPVVTLYEFEPAAGVKSSRVISLADDVARSMSAVAARIA
                     AIPGRNVLGIELPNQTRETVYLRELLASAEYEKARAPLILALGKTIGGDAVMADLAKM
                     PHLLIAGTTGSGKSVALNTMILSLLYRMPPDQCRMIMIDPKMLELSAYDGIPHLLAAV
                     VTDPKKAVVALKWAVREMEERYRKMSKLAVRGVEAFNERVKKAKAKGETLKRTVQTGF
                     DRETGKPIYEDEVLELEPMPYIVVVVDEMADLMMISGKEIEGAVQRLAQMARAAGIHL
                     IAATQRPSVDVITGTIKANFPTRISFQVTSKIDSRTILGEQGAEQLLGSGDMLYMMAG
                     GRIRRVHGPYVSDREVEDVVKFLKTQGTPDYLEAVTEE"
BASE COUNT          311 a          546 c          525 g          258 t
ORIGIN      
        1 ccctgtcgca gcatgccgga caaaactatc atcaggaatg gatcggctgg gcgatgccgg
       61 ttgtgctgtt ggccaccggc ctgacgttgg ccgtgctcgc ggccgatctg cgcctgaagc
      121 ccttgctgaa gggcgcggcc accacggcgg cgttgttcta ttggctcggc tcgcttctga
      181 agctgccggc ctttttcacc cgcaagccat acgacgacga cagcgacatc gaaccggacg
      241 tggaggacga aggggatttc ggcctcggca tcgctcccga accgctggct tcaagctcgc
      301 gcgaaaaccg ggtgcgccgc gaagaggcac gcaaggctcc gctcaagcgc gcgcgccagc
      361 ccgcgctcag tctggaatcc agcgagtacc agcttcccgc gctgggcctg ctggccgaac
      421 ccaagcatat cgccgacgcc accgcgcttt ccgacgagtc gctggaagaa aacgcgcgca
      481 tgctggaagc cgtgctcgcc gatttcggcg tcaagggccg cattgtcgcc gtgcggcccg
      541 gaccggtggt gacgctctac gaattcgagc cggcggcagg ggtgaaatcc tcgcgggtga
      601 tttcgctggc cgatgacgtg gcccgctcga tgagcgcggt ggcggcgcgc atcgccgcca
      661 ttcccggccg caacgtgctg ggcatcgaac tgcccaacca gacgcgcgag acggtttacc
      721 tgcgcgagct gctggccagc gccgaatacg aaaaagcgcg cgcgccgctg atcctggcgc
      781 tgggcaagac catcggcggc gacgcggtga tggccgacct cgccaagatg ccccacctct
      841 tgatcgccgg caccaccgga tcgggcaagt cggtggcgct caacaccatg atcttgtcgc
      901 tgctctaccg catgccgccg gaccagtgcc gcatgatcat gatcgatccc aagatgctgg
      961 aactgtcggc ttacgacggt attccgcatc tgctcgccgc cgtcgtcacc gatccgaaga
     1021 aggcggtggt cgcgctcaaa tgggcggtgc gcgagatgga agagcgctac cgcaaaatgt
     1081 ccaagctcgc cgtgcgcggc gtggaagcct tcaacgagcg cgtcaagaag gccaaggcca
     1141 agggcgagac cttgaagcgc acggtgcaga ccggcttcga ccgcgagacc ggcaagccga
     1201 tctacgaaga cgaagtgctc gaactggagc cgatgcccta catcgtcgtg gtggtcgacg
     1261 agatggcgga cctgatgatg atctccggca aggagatcga aggcgcggtg cagcgcctgg
     1321 cgcagatggc gcgcgccgcc ggcatccatc tgatcgccgc cacccagcgc ccttcggtgg
     1381 acgtcatcac cggcaccatc aaggccaact tccccacccg catctctttc caggtgacca
     1441 gcaagatcga cagccgcacc atcctgggcg agcaaggcgc cgagcaactg ctcggctccg
     1501 gcgacatgct ctatatgatg gcgggcgggc gcatccgccg cgtccacggc ccctatgtca
     1561 gcgaccgcga agtcgaagac gtggtgaagt tcctcaagac ccaaggcacg cccgattatc
     1621 tggaggccgt caccgaagaa
//
LOCUS       DASWPU010000050         1973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_110465,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000050 DASWPU010000000
VERSION     DASWPU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110465"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1973)
                     /locus_tag="VGC27_01045"
     CDS             complement(<1..>1973)
                     /locus_tag="VGC27_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0281192.1"
                     /translation="LFGGDGEDTASYASAASGVRVSLDYTLANSGDARGDAFHSIEDL
                     EGSAFNDTLIGGDSGNLLRGGAGNDTLRGGLGDDTYEYKRGDGNDVINDVGISGAEEV
                     LGADGSLNTSLYTASWESIGTTMVGGYTWYAYRLTVTRNGTGEVVYRSRDNVDFLYTT
                     ANQSLPSGAGWPFADGQWRLDAARTGNGVQTVRTLDGSAGGDDALLLDNITLADLTAS
                     RNGSDLILNIAGGGQITIKNQTNAATAVESLLLGDGLAASLTNLRLPGEAATDGDDLF
                     LGDANANTFDGGAGDDVISGGAGNDVLHGGAGNDTLEGGPGADILDGGSDSVTLAESP
                     DADNFAKPYGDTIRYAGSSSAVTINLKTLTASGGDAAGDTIVADGNGVSTIENVVGSD
                     AGNDVLTGDDRANRLSGLGGDDTLFGLGGDDVLIGGTGNDTLDGGDGNDNLAGQDGND
                     ILYGGLGNDLLDGGAGDDVLYGGSGTDTLVGGSGSDTLYGGDDNDQLSGGDGNDTLYG
                     GTGNDTLSGDAGDDHLEGGAGDDIYVFSASSGTDTVLDAEGKNRIVISGVSSSQIWLN
                     HSGNDLVIGVIGGTATIRILGYYASSAPSRMFSIAIGDQTLFLAYAEPLINAMSALYA
                     NAPASMPQAVGDILGDYWDTGSKAAPRVVAQSYT"
BASE COUNT          370 a          648 c          568 g          387 t
ORIGIN      
        1 gtatagctct gggcgacgac gcgcggagcc gccttgctgc ccgtgtccca gtaatctccc
       61 aggatatcgc caaccgcctg gggcattgaa gccggcgcat tcgcatacag ggccgacatc
      121 gcgttgatga gtggttcggc ataagccagg aagagcgtct gatcgccgat cgcgatgctg
      181 aacattcgtg atggcgcgct cgacgcgtag tagcccagaa tacggatcgt cgccgtccca
      241 ccgataacac cgatcacaag atcgttgccc gaatggttga gccagatttg cgagctgctg
      301 acgccggaaa ttacgattct gttctttcct tcggcatcca aaaccgtatc ggtgccggaa
      361 ctagcgctaa acacgtaaat atcgtcgccc gcaccgcctt ccaagtgatc gtccccagcg
      421 tcgccggaaa gcgtatcatt gccggtcccg ccgtacagtg tgtcgttacc gtcaccgccg
      481 ctcaactgat cgttatcgtc tccgccataa agcgtatcgg agccgctacc gcccacgagc
      541 gtatccgtgc ccgaacctcc gtagagcaca tcgtcgccgg ctccaccgtc cagcagatcg
      601 ttacccaaac cgccgtaaag aatatcgttg ccgtcttgac cggcgaggtt atcgttgccg
      661 tctccgccat caagcgtgtc gttgccggtt ccgccgatga gcacgtcatc gccccccagc
      721 ccgaaaagcg tgtcatcccc acccaagccc gaaagccggt tggcgcggtc gtcgccggtc
      781 agcacatcgt tgccggcgtc cgatccaacc acgttttcaa tcgtggagac gccattgcca
      841 tcggcgacaa tggtatcgcc cgcggcgtca ccacccgagg cggtgagagt tttcaaattg
      901 atcgtgactg cggaggacga acctgcgtag cggatcgtgt cgccataagg cttggcaaaa
      961 ttatcggcgt ctgggctctc ggccagcgtt acgctgtcgc tgccgccatc gaggatgtct
     1021 gcgcccggac cgccttccaa cgtgtcgttg cccgccccgc cgtggagcac atcgttgccg
     1081 gccccgccgg agatcacatc atcgccggcc ccgccatcga aggtgttcgc attggcgtcg
     1141 cccaggaaca ggtcatcgcc gtccgtggct gcttcgccgg gaaggcgcag attggtgaga
     1201 gaagccgcca acccatcgcc cagcagtagg ctttccacgg cggtagcagc attagtctgg
     1261 ttcttgatcg tgatctgacc gccgccggct atattgagga tcagatccga gccattccgg
     1321 ctggccgtca gatccgcaag cgtaatgttg tcgagtagaa gagcatcatc gccgccggcc
     1381 gaaccgtcga gtgtgcgcac cgtctgcacg ccgttgccgg tgcgtgcggc atcaagccgc
     1441 cattgtccgt ccgcgaacgg ccacccggcc cccgaaggca agctttggtt ggctgtcgtg
     1501 tagaggaaat ccacattgtc gcgcgaacga taaacgactt cgccagtgcc gttccgcgtg
     1561 accgtcagcc ggtaggcata ccaggtgtag ccgcccacca tagttgttcc aatgctttcc
     1621 caggaagcgg tgtaaagact agtattgagc gagccatccg cgcctaggac ttcctcggcg
     1681 ccggagattc cgacatcgtt gatgacgtca ttcccatccc cgcgcttgta ctcgtatgtg
     1741 tcgtcgccca aaccgccgcg cagcgtatca ttgccggcgc cgcctctgag cagattgccg
     1801 ctgtcgccgc caatcaaagt gtcgttgaag gcggaacctt cgaggtcctc gatgctgtgg
     1861 aacgcatcgc cgcgggcgtc gccgctgttg gcgagcgtat agtccagcga cacccgcacg
     1921 ccggacgcgg cggaggcata ggacgccgtg tcctcgccgt cgccgccgaa caa
//
LOCUS       DASWPU010000051         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_24791,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000051 DASWPU010000000
VERSION     DASWPU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24791"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VGC27_01050"
     CDS             complement(<1..1093)
                     /locus_tag="VGC27_01050"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY0281193.1"
                     /translation="MLDRRAVLRSGTAIAFLAAASEATAKAVPKSASPEAVKLNALFA
                     AFMNETLDRAPEFVTSLGLDKGRRAYQKSMLANRSPAEIAALKRLNASQLARLRAVKR
                     SALGGTDAVNVDVVLYGLQQQEETNRRFQFGAIGASAPYVLYQLGGAYQSIPDFLDSQ
                     HQIAGKSDADDYLARLAALGTVLDQEREAAAHDIGLGIVPPDFVLARTLEQMTKLRGQ
                     KAETSPLVESLARRVKEKKIAGDYAGQAARIVGEIVYPALDRQIALLKSLQPKAVHDA
                     GVWRLKDGEAYYAASLAAQTTTNMKPAEIHKLGLDLVKSQAAQMDALMKAGGLTKGTV
                     GARLRAMFDDPKFRYPNTDAGKEKLIADLN"
     gene            1792..>2181
                     /locus_tag="VGC27_01055"
     rRNA            1792..>2181
                     /locus_tag="VGC27_01055"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          423 a          611 c          713 g          434 t
ORIGIN      
        1 ggttcagatc ggcgatgagt ttttccttgc ccgcgtcggt gttgggatag cggaacttgg
       61 gatcgtcgaa catcgcgcgc agccgggcgc cgaccgtgcc cttggtcagt ccgcccgctt
      121 tcatcagggc atccatctgc gccgcctggc tcttcacgag gtcgaggccg agcttgtgga
      181 tttccgccgg tttcatgttc gtcgtcgtct gcgcggcgag cgaggcggca taataggctt
      241 cgccgtcttt cagccgccac acgccggcat cgtgtacggc cttaggctga aggcttttca
      301 acagtgcgat ctgccggtcg agcgcgggat agacgatctc gccgacgatg cgggcggcct
      361 gtccggcgta atcgccggcg atcttcttct ccttgacgcg gcgggcgagg gattcgacca
      421 gcggcgatgt ttccgctttc tggccgcgca gcttcgtcat ctgttcgagc gtgcgggcca
      481 gcacgaaatc gggcgggacg attccgagcc cgatgtcgtg cgcggcggct tcgcgttctt
      541 gatcgaggac ggtgcctaac gcggcgagcc gcgcgagata atcgtcggca tcggacttgc
      601 cggcgatctg gtgctggctg tcgaggaagt ccggtatgga ttggtaggcg ccgccgagct
      661 gatagaggac atagggcgcg ctcgcgccga tggcgccaaa ctggaagcgc cggttggttt
      721 cctcctgctg ctgcaagcca tagagcacga cgtcgacatt gaccgcatcc gtgccgccga
      781 gcgcgctgcg cttgacggca cgcaggcgcg cgagctggct cgcgttgagc cgtttgagcg
      841 ccgcaatttc cgccggcgag cggttcgcca gcatgctttt ctgataggcc cgcctgccct
      901 tgtccaagcc gaggctggtg acgaattccg gcgcccggtc cagggtctcg ttcatgaacg
      961 cagcgaacag cgcgttcagc ttgaccgctt cggggctggc ggatttgggc acggccttgg
     1021 ccgttgcctc gctggctgcg gcaaggaaag cgatggcggt tccggaacga agaacggcgc
     1081 ggcggtcgag catgatggtc ccccatgtgc agaggagaga gctttaatcg gcgcggcgcg
     1141 aggccacaac ccggcctttt gggctgcgaa atgccggggg ccgcgtcaac aggccgaaaa
     1201 cgccgcagtg ctgcgcggcc cttccgggcg cgacacttgt tcgcttttgc acatcggcgg
     1261 ctagggagcc cggaatttgc cggttccgaa gccctataga tatgggcgca gatcaatatg
     1321 ccgccacaac gggttgagca atttcgttgc ggcgggaatc gccatgatgc ggcgcaaaat
     1381 atcaatccac aggagcgcga caaagcgcgg aaaaaaagca aataaaacat gaattagggg
     1441 tttgacaggc tattgaggcg cgcctataca ccccgcccac gccgcccgct gggccaagtc
     1501 ttgcgggcgg tgttctgtct gaaccggaag cgatttttcg cctcaggcgg cgctctttga
     1561 cattgtgatt taaggaaggg gaacgtgggc ggcggctcgg cttgccacgc gtcggatcta
     1621 gcatccggcg tgaagcaata tcgagcaact cagttgaatg ctcgcgttcc tttgcaaaca
     1681 catgaaagat gttcgattgt ttcggagctg tgacggcgcc gaagcagtca aacctcgtca
     1741 aacgtgtaag cgaactaacg caatagcatt cagcgtcgtt gcttaggatc ataacttgag
     1801 agtttgatcc tggctcagaa cgaacgctgg cggcaggcct caaacatgca agtctagggg
     1861 gcgtagcaat acgtcaccgg cggacgggtg cgtaacacgt gggaatgtac ctattggttc
     1921 ggaacaacgc agggaaactt gcgctaatac cggatgtgcc cgaaagggga aagatttatc
     1981 gccaatagat cagcccgcgt aggattagct agttggtagg gtaacggcct accaaggcaa
     2041 cgatccttag ctgttctgag aggaagatca gccacactgg gactgagaca cggcccagac
     2101 tcctacggga ggcagcagtt gggaatcttg gacaatgggg gaaaccctga tccagccatg
     2161 ccgcgtgagt gatgaaggcc t
//
LOCUS       DASWPU010000052         4310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_374, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000052 DASWPU010000000
VERSION     DASWPU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..2389
                     /locus_tag="VGC27_01060"
     CDS             314..2389
                     /locus_tag="VGC27_01060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY0281194.1"
                     /translation="MTIQSAAAPNEMPLLAPWKGPHGGVPAFAGVKTADFKPALLEGI
                     ERNRAEIAKIAADKAPPSFENTIAALEDTGRPLGRAASIFYIYVSTMNDKEMQAVEKE
                     MSPILAAFNDEVIQNAALFARINAIYQARENAGLTPEQQRLVWVIYRRFTREGAALAA
                     ADKARLAELNQQLAALYTAFGQNVLGDEEDHWLALEEAELDGLPDWLRASAAAAAEKK
                     TQTGKFLFANTRSAIEPFLTYSTRRDLREKAFRMWTSRGDNGDAHDNNAAIAQILQLR
                     AEKAKLLGYPTFAHWVTDYQMAKTPDAAMDLLLKVWAPAAARVREEVADMQKIADSEG
                     AGIKIAPWDYRFYAEKVRKAKYDLDESEIKPYLQLEKIREGMFWAAGQLYGLEFVQVH
                     DLPVYRSDVRVFDVRRGGKHVGLWYFDPYAHTGKSSGAWMNEYRAQERFRGVVTPIVS
                     NNTNFVPGAPGEPILISWDDATTMFHEFGHALHGLNSNVTYPTLAGSNVVRDFVEFPS
                     QLNEHWLPTHQVLSRFALHYKTGKPMPDALVAKIKQARTFNQGFTTVEYLASAIVDMK
                     AHLAGAEKIDPRAFEAAALKQIGMPEEIVMRHRLPHFGHIFSGEGYASGYYDYIWADT
                     LVADAAEAFEEAPGGYYDKALAKKLYDGIVSRGNTVDAGEAYRKFRGRDATVDALMRD
                     RGFPAPPKT"
     gene            complement(2422..3138)
                     /locus_tag="VGC27_01065"
     CDS             complement(2422..3138)
                     /locus_tag="VGC27_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0281195.1"
                     /translation="MLDVFKPGAKRVVNSALSVLPAQARKRFKEYHELRYWRGVTRPI
                     AHDRAKLEHERSHYEFFFTTFFGLDAGDYAGAAILDIGCGPCGSLEWAAGARERVGLD
                     PLADRYRGLAEDKQAMSYCAASSEAIPYPDGHFDVVSSFNSLDHVDDVEATIREIKRV
                     TAPAGRILLIVEIGHAPTPTEPHWLDESVVAKFAPEFAPTGVRLFGVREDHNLYASLE
                     HAIPYVEGNPGVLAARLERA"
     gene            complement(3328..3413)
                     /locus_tag="VGC27_01070"
     tRNA            complement(3328..3413)
                     /locus_tag="VGC27_01070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3378..3380),aa:Leu,seq:caa)
     gene            complement(3492..3914)
                     /locus_tag="VGC27_01075"
     CDS             complement(3492..3914)
                     /locus_tag="VGC27_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281196.1"
                     /translation="MNENRKQILDMLAQGKITADEAERLIAALEAAPAEAAPRAGAPK
                     YLRVLVEADEETGGEEPTKVNIRVPIKLMRAGVRLAGLIPQEARGHINEAMREKGMDF
                     DISQLRPENLDELIDNLQDLSIDVDQKAHKAKVKVFCE"
     gene            complement(3924..4184)
                     /locus_tag="VGC27_01080"
     CDS             complement(3924..4184)
                     /locus_tag="VGC27_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281197.1"
                     /translation="MIPLWMSFHLRTPRGTSLRLGLPLFLIWLLLLPLAIVIAPFALI
                     ACVLLRFSPLGCIAFIWGLLSAARGTHLEIEAPDGAVFLHLY"
BASE COUNT          801 a         1412 c         1379 g          718 t
ORIGIN      
        1 ggccgaacac gcacggccgt tccagcgcgt gtcgcgatcg tccggtgccg agaatacgcc
       61 ttgccgggcg gggccgctgc cggagggccc cgcccccgcc tcggcttatt gtcgcacctc
      121 ggatgcgact ttattgcccg gcagcggcgg ccggatccaa tagctccaca cggaacgttg
      181 agttgaacct cttgatggaa agacgcattc ttcccggcag cgcaattgtc tccaggggga
      241 tgacagacac atgcaaagac gcacttttct gctagcttcg gccgcagcag ccgccaccac
      301 ctcgcttttg tccatgacga ttcagtccgc cgccgcgccg aacgagatgc ctttgctggc
      361 gccctggaag ggaccccatg gcggcgtgcc ggcgtttgcc ggagtgaaga ccgcggattt
      421 caagccggcg ctgctcgaag gcatcgaacg caaccgcgcc gaaatcgcca agatcgccgc
      481 cgacaaggcc ccgcccagtt ttgaaaacac catcgccgcg ctggaggata ccggccgccc
      541 gctcggccgc gccgcatcga tcttctacat ctacgtctcg acgatgaacg acaaggagat
      601 gcaggcggtc gagaaggaga tgtcgcccat cctcgccgcc ttcaacgacg aagtgatcca
      661 gaacgcggcg ctgttcgccc gcatcaacgc gatctaccag gcgcgcgaaa acgccggact
      721 gacgcccgaa cagcagcgcc tggtctgggt gatctatcgc cgcttcaccc gtgaaggcgc
      781 cgcgctcgcc gcggcggaca aggccaggct cgccgagctc aaccagcaac tcgccgcgct
      841 ctacaccgcg ttcggccaga acgtattggg cgacgaggaa gaccattggc tggcgctcga
      901 ggaagccgaa ctcgacggcc tgccggattg gctgcgcgcg tcggcggcgg ccgcggcgga
      961 aaagaagacg cagacgggca aattcctctt cgccaacacg cgctcggcga tcgagccctt
     1021 cctcacctat tccacccgcc gcgacctgcg cgaaaaggcg ttccggatgt ggacctcgcg
     1081 cggcgacaat ggcgacgcgc acgacaacaa cgccgccatc gcgcaaatcc tgcaattgcg
     1141 cgccgagaag gccaagctgc tcggctatcc cactttcgcc cattgggtca cggactatca
     1201 gatggcgaag acgccggacg ccgccatgga cctgttgctg aaggtgtggg cgccggcggc
     1261 ggcgcgggtg cgcgaggaag tcgccgacat gcagaagatc gccgattccg agggcgcggg
     1321 aatcaagatc gcgccgtggg attaccgttt ctatgccgag aaggtgcgca aggcgaaata
     1381 cgatctcgac gaaagcgaaa tcaagccata cctccagctc gaaaaaatcc gcgaaggcat
     1441 gttctgggcg gcgggccagc tctacgggtt ggaattcgtc caggttcacg acctgccggt
     1501 ctatcgcagc gacgtgcgcg tgttcgacgt gcgccgcggc ggcaagcatg tgggcctgtg
     1561 gtacttcgat ccctacgcgc acacgggcaa atcgtccggc gcctggatga acgaataccg
     1621 cgcccaggag cgtttccgcg gcgtggtcac gccgatcgtt tccaacaaca ccaatttcgt
     1681 tccgggcgcg ccgggcgagc cgatcctgat ttcgtgggac gacgccacca ccatgttcca
     1741 cgaattcggc cacgccttgc acgggctgaa ttcgaacgtc acctatccca cgctggcggg
     1801 cagcaatgtg gtgcgcgact tcgtggagtt ccccagccag ctcaacgagc attggctgcc
     1861 gacccaccag gtgctgtcgc gcttcgccct gcattacaag accggcaagc ccatgcccga
     1921 tgcgctggtc gccaagatca agcaggcgcg caccttcaac cagggcttca ccacggtgga
     1981 atatctggcg tcggccatcg tcgacatgaa ggcgcatctg gcgggggccg agaagatcga
     2041 tccgcgcgcc ttcgaggcgg ccgccttgaa gcaaatcggc atgccggagg aaatcgtcat
     2101 gcgccatcgc ctgccgcatt tcgggcacat cttttcgggc gagggctatg cgtcgggcta
     2161 ttacgattac atctgggccg acacgctggt cgccgatgcc gcggaggcgt tcgaggaagc
     2221 gccgggcggc tactacgaca aggcgctcgc caagaagctg tacgacggca tcgtcagccg
     2281 cggcaacacg gtcgatgcgg gcgaagccta ccgcaagttc cgcggccgcg acgccaccgt
     2341 cgatgcgctg atgcgcgacc ggggatttcc ggcgccgccc aagacgtgag gcaaacgcct
     2401 aggcgacgtg tctggcgccg gtcaggcgcg ttccagccgc gccgcaagaa cgccgggatt
     2461 gccctcgacg tagggaatgg cgtgttcgag cgaggcatag aggttgtgat cctcgcggac
     2521 gccgaacagg cgaacgcctg tgggcgcgaa ttcgggcgcg aactttgcga ccacgctttc
     2581 gtccagccaa tgcggttcgg tgggcgtcgg cgcgtggccg atctcgacga tcagcaggat
     2641 gcgtccggcg ggcgccgtca cccgcttgat ttcgcggatc gtcgcctcga catcgtcgac
     2701 gtgatccagg ctgttgaagc tcgaaacgac atcgaaatgg ccgtcgggat aggggatcgc
     2761 ttccgacgaa gcggcgcaat agctcatcgc ctgcttgtct tcggcaagcc cgcggtaacg
     2821 gtcggccaag ggatcgaggc cgacgcgttc gcgcgcgccc gccgcccatt cgaggctgcc
     2881 gcaggggccg cagccgatgt cgagaatggc ggcgcccgcg taatcgccgg cgtcgagccc
     2941 gaagaacgtc gtgaagaaga attcgtaatg cgagcgctca tgctctagct tcgcccggtc
     3001 gtgcgcgatg ggccgggtga cgccccgcca gtagcgcagt tcgtgatatt ccttgaaccg
     3061 cttgcgcgcc tgtgcgggca ggacggacag cgccgaatta accacccgct tcgcgcccgg
     3121 tttgaagacg tcaagcattc cgcatccttt cttgcacaga gccgccgtgc caaaacggtt
     3181 caaagccggc ggcgaagaga tacggagcaa aaacgaggcc agcggcgcag gcggcccgcc
     3241 aagtcgcagc cgaacgatcc caggcagccc ggcttcgttg ctgcgcaact gcgccgtggc
     3301 agtctccgcc gctagcgcgg cgtagactgg tgcccctggc cggacttgaa ccagcacgcc
     3361 cgtgaaggca acagattttg agtctgccgc gtctaccatt ccgccacagg ggcacgtttc
     3421 gcggttaaca agcgaatgga ggcgcggatc atagccaggc cgggcgttcg gtcaatgcgc
     3481 accctggcgc gctattcgca gaacaccttg accttcgcct tgtgggcttt ctggtccacg
     3541 tcgatcgaca gatcttgcag attgtcgatc agttcgtcga ggttctccgg ccgcaactgg
     3601 ctgatgtcga agtccatgcc cttctcgcgc atggcctcgt tgatatggcc gcgcgcctcc
     3661 tgggggatca gaccggcgag tctcaccccg gcgcgcatca gcttgatcgg cacgcggata
     3721 ttcaccttgg tcggttcttc gccgcccgtt tcctcgtccg cctccaccag cacgcgcaga
     3781 tatttcggtg caccggcgcg cggcgcggct tcggcgggcg cggcctccag cgccgcgatc
     3841 agccgctcgg cctcatcggc cgtgatcttg ccctgggcca gcatgtccag gatctgcttg
     3901 cggttctcgt tcatggcatt ctcctaataa agatgcagga aaacggcgcc gtccggcgcc
     3961 tcgatttcga gatgagtgcc tctggcggcg ctgagcaggc cccagatgaa ggcgatgcaa
     4021 cccagcgggc tgaaacgcag cagcacgcaa gcgatcagcg cgaagggcgc gatcacgatc
     4081 gccaagggca gcagcagcag ccagatcagg aacagcggca ggccgagccg caagctggtg
     4141 ccgcgtggcg tgcgcagatg aaaactcatc cagagcggga tcatagggcc tccagggcgg
     4201 cgatggcctc tccggcgctt atctcgccgc gcgcgaggcg ttcgaggatt tcgccgcgcg
     4261 gcggtaccgg atcggtctcg acgaagtcga gctgcgccga aatgcggttc
//
LOCUS       DASWPU010000053         4531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_43320,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000053 DASWPU010000000
VERSION     DASWPU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43320"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..792
                     /locus_tag="VGC27_01085"
     CDS             199..792
                     /locus_tag="VGC27_01085"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEY0281198.1"
                     /translation="MPASQRKIVPADILSDDEYARQRDALRKEAIAVKKNRRVEVGPY
                     ATFYFENYDTMWQQVMEMLRIEKGGAEQAAGELETYNPLIPQGRELIATLMLEIEDTV
                     IRDRTLLTLGGIEETVFLDIGKDRIKATPTEYEDRTTPEGKTSSVHWLRFAFTPEQID
                     RFKRGEDRVMLGVAHTHYGHMAVMPGAVRAELAKDFA"
     gene            1032..1559
                     /locus_tag="VGC27_01090"
     CDS             1032..1559
                     /locus_tag="VGC27_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281199.1"
                     /translation="MPAIDYSSHVNRTAPAPPPAAAKKAETPHFSFGDLLDIINPLQH
                     IPVVSTLYRAISGDGIGVPEKIAGDTLYGGVIGLVVSLADTAYKGITGEYAGDTVLAF
                     LTGDGTDAPVQVAAAPETPAQVTPAQLTDAPDLSALMGALSRKGVDAGTAARAASAYR
                     FTIGLTARAPLLPQS"
     gene            complement(1556..2224)
                     /locus_tag="VGC27_01095"
     CDS             complement(1556..2224)
                     /locus_tag="VGC27_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY0281200.1"
                     /translation="MVPEQIARNFALILARIEAARKSALKPAPITRLIAVSKFHDIGH
                     IRPLLEAGHRLFGESRVQEALAKWLPLKSEFPDIELHLIGPLQTNKAREAVGLFDAIH
                     SLDRPKLAQVLKSESERAGRSPMLFVQVNTGEEPQKAGVAPKDTAALIAEARVLGLNL
                     QGLMCIPPVGAAPAPHFALLAKLAAEQNLPLLSMGMSDDFKTAIRFGATHVRVGSAIF
                     GERD"
     gene            2228..2836
                     /locus_tag="VGC27_01100"
     CDS             2228..2836
                     /locus_tag="VGC27_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0281201.1"
                     /translation="MSDSLARRRLARAAARLAAHARHALPPLALFTDDDRLADPLAAA
                     RALPRGSLVVVRARGGARRAALARAMAELARSRDLIVLVAGDGALASACGADGIHLPE
                     ARLGEAAGWRARRNFLITGSVHSFAGLARATWLDAVFLSPVFPTASHPGRTALTSMRA
                     NAIARGARLPVYALGGIEPRNAGLLSGFAGIAAIGALECDPI"
     gene            complement(2936..4099)
                     /locus_tag="VGC27_01105"
     CDS             complement(2936..4099)
                     /locus_tag="VGC27_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281202.1"
                     /translation="MRAFLLAGMAAMIAAPASASDLGSFNPWTTNLSNLSLTVGGRAQ
                     GTLFTAAAGIDETRGSGAAALTASLQRDYDSGLTLALKGAFEIYHDRLSGDNYGDDLV
                     QKVYAVAQTGLGRVEIGMTDGAVYALAVSGPVVDRDTSLDNANATFFRDPSTGRAFIN
                     VFRLDSAAESSLNYAKISYYTPRLFGVQLAASYTPSEGKDVIPFLSSGPRVANRQESI
                     WEAVLSYSGTFGPVALGAYGGVVFAHADGATKTAGHEGLTEWSAGSEIDYNLNDDVKL
                     AVGGGYRRSNAYAFDINNVLADGATSSAHLSATVSSGPWIVGGEVGEGTAEGAPKLGV
                     QGVSASVGYVFNANWQATLGWQQLLYKSDAGTFYNGAPRIRMNAAFLHLSLRV"
BASE COUNT          797 a         1447 c         1431 g          856 t
ORIGIN      
        1 atggcccacg cctatgggct gatctgattg aatcgtttcc cctcaccctt cgacaagctc
       61 agggtgaggg gattttaccg tccgtcatgc tgagcttgtc gaagcatgag cttgtcgaag
      121 catgagtttg tcgaagcata agcgtgtcga agcctgagag ccatgcgatc cccttggatc
      181 gcccgcatgg agagccaaat gcccgcaagt caacgtaaga tcgttcccgc cgacattctt
      241 tccgacgacg aatatgcacg gcaacgcgac gcgctgcgca aggaggccat cgcggtcaag
      301 aagaaccgcc gggtggaagt cggcccctac gccacctttt atttcgagaa ttacgatacg
      361 atgtggcagc aggtgatgga gatgctgcgc atcgaaaagg gcggcgccga gcaagcggcg
      421 ggcgaattgg aaacctataa tccgctgatc ccgcaaggac gcgagctgat tgccacgctg
      481 atgctggaga tcgaggatac ggtcatccgc gaccggacgc ttctgaccct cggcggcatc
      541 gaggaaacgg tcttcctcga catcggcaag gaccgcatca aggcgacgcc gaccgagtac
      601 gaagaccgca ctaccccgga aggcaaaacc tcttcggtgc attggctgcg ctttgccttc
      661 acgccggaac agatcgaccg cttcaagcgc ggcgaagaca gggtgatgct gggcgtcgcg
      721 cacacccatt acggccacat ggcggtgatg cccggcgcgg tgcgcgcgga attggcgaag
      781 gatttcgcct aaagcatttt tcagggaagt ggaatccggt tttccgtccg aaaacgcgac
      841 aaattaagaa cttggaacct ttcagcgttt ccgtgaaaca ctgaaggctc tggaacgaat
      901 tcgacaccgg gttttcatgc gccatcttga cgccgatccg ctcttagccg gcaatttttg
      961 cccgctgtgt gaagacgccg cgccggaaat caaggctccg tctctggcaa ggtgcttgct
     1021 tgaaggcatc catgccggcg atcgattact ccagccatgt gaataggacg gcgccagcgc
     1081 cgccgccggc ggctgccaaa aaagccgaga cgccgcattt ctccttcggc gaccttctgg
     1141 acatcatcaa tccgctccag catatccccg tggtttcgac cctctaccgt gccatcagcg
     1201 gcgacgggat cggcgtgccc gagaagatcg cgggcgatac gctgtacggc ggcgtcatcg
     1261 gcctcgtcgt ctcgctggcc gacacggcct acaaagggat caccggcgaa tatgccggcg
     1321 atacggtgct ggcgttcctg accggcgacg gcaccgatgc tcccgtccaa gtcgccgccg
     1381 cgcccgaaac gccggcgcag gtgacgccgg cgcagttaac ggacgcgccc gacctgtccg
     1441 cgttgatggg cgcactgtcc cgcaagggtg tcgatgccgg caccgccgcg cgcgccgcct
     1501 cggcctatcg cttcaccatc ggcttgacgg cacgagcccc gctgctgccg cagagctaat
     1561 cccgctcgcc gaagatcgcg ctgcccaccc gcacatgcgt ggcgccgaag cggatcgcgg
     1621 ttttgaaatc gtcactcatg cccatgctga gcagcggcag attttgttcc gcggcgagct
     1681 tcgccagcag cgcgaaatgc ggcgcgggcg cggcgccgac cggcgggatg cacatcaatc
     1741 cttggaggtt cagtcccaat acgcgcgcct cggcgatcag ggcggcggta tccttgggcg
     1801 ccacgcccgc cttttgcggc tcctcgccgg tgttgacctg cacgaacagc atgggcgagc
     1861 gcccggcccg ttcgctttcg ctcttcagca cctgagcgag cttcggccgg tcgagcgaat
     1921 ggatcgcgtc gaacagaccg acggcctcgc gcgccttgtt ggtctgcaac ggcccgatca
     1981 gatgcagttc gatgtcgggg aattcgctct tcaaaggcag ccatttcgcc agcgcttcct
     2041 gcacccggct ttcgccgaac aggcggtggc cggcttcgag cagcggacgg atatgcccga
     2101 tatcgtggaa cttgctgacg gcgatcagcc tggtaatggg ggcaggcttg agcgcgctct
     2161 ttcgcgccgc ctcgatgcgc gccaggataa gggcgaaatt ccgcgcgatt tgttccggaa
     2221 ccatggcatg agcgatagct tggcaaggcg gagactggca agagcggcgg cacggctggc
     2281 tgcgcatgcc cgccatgccc tgccgcctct ggccctgttc accgacgacg accgcctggc
     2341 cgatcccttg gcggcggccc gtgccttgcc gcgcggcagc ctggtggtgg tgcgcgcccg
     2401 cggcggcgcc cgccgcgccg ctttggcccg ggcgatggcc gagctggcgc gcagccgtga
     2461 cttgatcgtg ctcgtcgccg gcgacggcgc cctggcgtcc gcttgcgggg cggacggcat
     2521 tcatctgcct gaggcgcgcc tcggcgaggc ggccggctgg cgcgcgcgcc gcaattttct
     2581 catcaccggg tcggtgcatt ccttcgcagg gctggccagg gcgacttggc tcgacgcggt
     2641 ttttctttcg ccggtctttc ccacggcaag ccatcccggc cgcaccgcgc tgacgtccat
     2701 gcgcgccaat gcgattgcgc gcggcgcgcg ccttccggtc tatgcgctgg gcggcataga
     2761 gccgcgcaac gccggcctgt tgtcgggctt tgccggcatc gcggcgattg gtgcgctaga
     2821 gtgtgatccg atttaattga atcggatcac actctcgatt tcttgttttt cgcgcaatct
     2881 gacggcaaac cgcgtcggca cgctgctgct accgaccact ttgccggatt gcgctctaga
     2941 cgcgcaggct taggtgcagg aatgccgcgt tcatgcggat gcgcggcgcg ccgttgtaga
     3001 aggttcccgc gtcgcttttg tagagcagct gttgccagcc cagcgtcgcc tgccaattgg
     3061 cgttgaaaac gtaaccgacg gaggcggaga cgccttgcac gccgagcttg ggcgcgcctt
     3121 cggcggtgcc ctcgccaacc tcgccgccga cgatccacgg cccgctgctc accgtcgcgc
     3181 tgagatgagc gctggaggtt gcgccgtccg ccagcacgtt gttgatgtcg aaggcatagg
     3241 cgttgctgcg gcggtagccg ccgccgacgg cgagcttcac atcgtcgttg agattatagt
     3301 caatttcgct gccggcgctc cattcggtga gcccttcatg gcctgcggtc ttggtggcgc
     3361 cgtcggcatg ggcgaatacg acgccgccgt aagcgccaag cgcgaccggc ccgaaagtgc
     3421 cgctatagct gagcaccgct tcccagatgc tctcttgccg gttcgccacg cgcggcccgc
     3481 tggagaggaa ggggatcacg tcctttcctt ccgagggcgt ataggatgcg gcaagctgca
     3541 cgccgaacaa tcggggcgtg taataggaaa tcttggcgta gttgagcgag gattcggcgg
     3601 cgctatcgag cctgaagacg ttgatgaaag ccctgccggt ggacggatcg cggaaaaagg
     3661 tggcgttggc gttgtccagg gacgtgtctc tgtcgaccac cggcccgctg accgccagcg
     3721 catagaccgc tccgtcggtc atgccgattt cgacgcggcc cagccccgtc tgcgccaccg
     3781 catagacttt ctgcaccaaa tcgtcgccat aattgtcgcc cgaaagccgg tcgtgataga
     3841 tctcgaacgc gcctttcagc gccagggtca ggccgctgtc ataatcgcgc tgcaaggaag
     3901 cggtcagcgc tgccgcgcca ctccccctgg tttcgtcgat tccggcggca gccgtgaaaa
     3961 gggttccttg cgcccgcccg ccgaccgtca agctgaggtt cgagaggttt gtcgtccaag
     4021 gattgaaaga ccccagatcg gaggcggagg cgggcgcggc gatcatggcg gccataccgg
     4081 caagcagaaa ggctctcatg accactccta aaacccgttt cggcggcatt tccggcggca
     4141 aacggctgca tgctccggat cgaagccggc cggaaaatgc tccgtacggc ccttttaccg
     4201 gacctcactc tgcttcttac cttaaacctt cataacgtaa gatgatctgg gctgcggcgc
     4261 gttgcacgcc gggctgcggc ccagtataag ccaaacccgc gtgaaaatcg gggtatgcgc
     4321 gggacggaag acttgtctcg ccctaataag gatgaagagc ggcccgatgc ggattttaac
     4381 cctagttgcg tgcggcctgc tgttggcggg ttgcggctcg tccaacccca acgtcataca
     4441 ggacatcgat tccggcacca ccgtcatatc cagcgacagc ggtcagcgca ccctgggtgt
     4501 gaattcctat ttgtggcatg cggccttgga c
//
LOCUS       DASWPU010000054         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_55514,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000054 DASWPU010000000
VERSION     DASWPU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55514"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VGC27_01110"
     CDS             <1..584
                     /locus_tag="VGC27_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0281203.1"
                     /translation="GFEGETSSIASARKQFGAIARRYGALPLGRALGEQWRQSRFQAP
                     YLRDPMMDRGLGIATLETAANWSKLGALYAATRAALEAAILKTVPREGAKGIVMCRIG
                     HSRVDGASLHFTAIFPRSLDGDIAQWKAIKQAASEAIMANGGTISHHHGVGEDHLPWI
                     AQEKGALGIEVLRAVKQVLDPAGVLNPGKLLPG"
     gene            686..1444
                     /locus_tag="VGC27_01115"
     CDS             686..1444
                     /locus_tag="VGC27_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281204.1"
                     /translation="MYRRLVRTEVRFFTLPEAMPRVDILKLRCLSAPYPGVENESLPT
                     VVIDLAPPEDALWNGVDVQTRKAIRQAEREGVQVGRVSELSAELWNRFYAAYQKLRSR
                     KKAADPLGIGQISELIERDSYVMTTSRDANGTILSWHGYVYGSGKVRLVNTISDIDPS
                     RGTQWNNLIGRAHRLHHWKDMLLFKEDGAETYDLGGVYRGTADQEQINIAKFKMRFGG
                     SPAETYDAVLPLTVKGRFALLLLAKISAEARSGG"
BASE COUNT          318 a          466 c          504 g          287 t
ORIGIN      
        1 cgggtttcga aggcgagaca tcatccatcg cgtcggcgcg aaagcaattc ggcgccattg
       61 cgcgcaggta cggcgcgctg ccgctgggcc gcgccttggg cgagcaatgg cggcagtccc
      121 gtttccaagc gccttatctg cgcgacccca tgatggaccg tggcctggga atcgcaacgc
      181 tggagaccgc cgcgaactgg agcaagctcg gcgcgttgta cgccgcaaca cgcgcggcgc
      241 tcgaagccgc gatcctcaag accgttccac gcgaaggcgc caaggggatc gtaatgtgcc
      301 gcatcggcca ttcccgagtt gacggcgcca gcctgcattt cacggccatc ttcccgcgct
      361 ccctcgacgg cgacatcgcg caatggaaag cgatcaagca ggcggcgagc gaggcgatca
      421 tggcgaacgg cgggacgatc agccatcatc acggtgtcgg cgaggatcat ttaccctgga
      481 tcgcgcagga aaaaggcgcg ctcggcatcg aggtgctgcg cgcggtcaag caggtgctcg
      541 atccggccgg tgtgctcaac ccagggaagc ttctgcccgg atagaatctg ggagacttag
      601 gcgaatcgca tatgttcttt accgaagtcg cgccacaagg accttatcga tgccgtttgt
      661 tccgaaggca tccctagctt ggagaatgta ccggcggctg gtgcgcaccg aggtccggtt
      721 tttcacgctg cccgaagcca tgccccgcgt cgatattctg aagctgcgct gtttgtccgc
      781 gccctatccg ggcgtcgaga acgagagttt gccgaccgtc gtgatcgacc ttgcgccgcc
      841 ggaagatgcg ctgtggaacg gtgtggacgt ccagaccaga aaagccatcc ggcaggccga
      901 gcgcgagggc gttcaggtcg ggcgggtgtc cgaattgtcc gcggagttat ggaatagatt
      961 ctacgccgcc tatcagaaac tcaggagccg caagaaggcg gcggacccgc tgggaatcgg
     1021 gcagatcagc gagttgatcg aaagagactc ctacgtgatg accacgagcc gcgacgcgaa
     1081 cggaactata ttgtcgtggc acggctatgt gtacggcagc ggtaaagtgc gcctggtcaa
     1141 cacgatttct gacatcgacc cttcgcgcgg cactcagtgg aacaatctga tcggacgcgc
     1201 gcatcgtctc caccattgga aggacatgct tcttttcaag gaagacggcg ccgagactta
     1261 cgatctcggc ggcgtctatc gcggaaccgc ggaccaggaa cagatcaaca tcgccaagtt
     1321 caagatgaga tttggcggca gcccggccga gacctacgat gccgtcttgc cgttgaccgt
     1381 caagggacgg tttgctctgc ttctccttgc gaagatcagc gccgaggcgc gctccggcgg
     1441 atgaggaaaa gaccatgaaa atctgtctga tgttgcatgg gctgggagcc ctgccgccgg
     1501 gcatttccga ggacgaagcg cgctattggc tgccggtgga gaagttcgcc tatatcctcg
     1561 gactcatccg gcgct
//
LOCUS       DASWPU010000055         6769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_98221,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000055 DASWPU010000000
VERSION     DASWPU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98221"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /gene="pyk"
                     /locus_tag="VGC27_01120"
     CDS             <1..1236
                     /gene="pyk"
                     /locus_tag="VGC27_01120"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY0281205.1"
                     /translation="ILVDMQGPKIRLGTLAGGVREVHNGDRLRLVRKVAAENETDIPI
                     PHAEVFAALRPGENILIDDGKVRLKIEEVNKDWANAVVEAGGLLKDKKGVNLPDTILP
                     IPAMTPKDRSDVDAALHLGVDWIALSFVQRPDDVAELKKVVGGRAACLAKIEKPRAIE
                     WLEDILDISDALMVARGDLGVELPVQEVPGKQKFITRAARKAGKPVVVATQMLESMIT
                     APVPTRAEVSDVATAVFEGADAVMLSAESAAGKYPAQAVAMMDKIATTVEGDPLYVSI
                     IDAQRFAPEATTADAIMAAVHEVTHTIHARAIVCWTKSGATALRAARERSDAPIIALT
                     PSLEISRQLSLVWGLHSVYTEDAQDLDDMVERAARFAAQEGFAKSGDRIVVTAGVPLR
                     TPGATNMLRVAFVGSQKNE"
     gene            complement(1286..1825)
                     /locus_tag="VGC27_01125"
     CDS             complement(1286..1825)
                     /locus_tag="VGC27_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281206.1"
                     /translation="MRLAIFLLALLLPAAAWAAPPSPLDALFAQLRAAGTPEDAKPIE
                     EQILAHFRQSGSPSIDLLMARAAAAQGAGDRDTARQLLDAVTNIAANYAEGWHRRALL
                     QAETGDDEGAMVSLQKTIALNPRQFEAMEQLGEMLEAYGDKEGALRMYRRALALNPQF
                     EGLQRHVDALSRAVEGQGI"
     gene            complement(1916..2041)
                     /gene="ykgO"
                     /locus_tag="VGC27_01130"
     CDS             complement(1916..2041)
                     /gene="ykgO"
                     /locus_tag="VGC27_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEY0281207.1"
                     /translation="MKVRNSLRSLKKRDKDCRVIRRRGRTYVINKKNPRFKARQG"
     gene            2222..3505
                     /locus_tag="VGC27_01135"
     CDS             2222..3505
                     /locus_tag="VGC27_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY0281208.1"
                     /translation="MRLILLFLAYILSLIIMAAAYGAIAAEAPPVSAPLAAAAVPVPA
                     PVPDENLAFARFVRDFRATALAAGIAPEIYDAAMSRIARNQRVVDLNLNQPEFTAPVW
                     SYLDNAVSPQRIAGGRAVLAGNAAILAPIVAKYRVPKEILVSIWGNESDYGRSMGRFN
                     LFEALATLAYDGPRIDYARPQLIAALQMMQRQKFAAADMMSSWAGAFGQTQFVPTTFL
                     AQAVDGDGDGKVDLWNSVADALASAARVISDAGWREGKPWGYEVVLPGGFAYEDADLD
                     NALPLAVWKKHGVKTAAGGTLPASDEPASIYLPAGARGPAFFVFSNFKTILKYNNAAS
                     YALAVCLLADQLQGIAPVAASWPRDQAALNRNERLALQTSLAKLGHDIGKIDGFIGSK
                     SRAAIRAWQKKHGLPADGFATLDLFTRIAMDAKGQ"
     gene            complement(3627..4712)
                     /locus_tag="VGC27_01140"
     CDS             complement(3627..4712)
                     /locus_tag="VGC27_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEY0281209.1"
                     /translation="MRPFSSASRIGAVVGVVLAAALAGCASISPEQPKEAVFPEQPYV
                     PIDAFAQNTQMGRGVAVLSEDPGWNDPAKGRFTPRHFKLIRDAGFDTVRIVLTAFDHM
                     DAAYTLDPRYLERLDLMVKAALAQDLNVVIDEHDYELCGKDVVVCKAKLNAFWSQVAP
                     RYKDAPNKLVFEMLNEPHEALTPELWNEQLVETLAIIRATNPTRNVVIGPGFWNGLEL
                     LPKLELPADDQHIIATVHYYHPMEFTHQGAAWTPQYEQLGVTWGSDADFALLNKEFDI
                     VKAWSEAHNRPIFLGEFGAYENGATADRVRWTSAVVRGAEARGFSWAYWQFDKDFIVY
                     DIDKDQWVEPILHALIPPNDKVSDATH"
     gene            complement(4820..>6769)
                     /gene="pbpC"
                     /locus_tag="VGC27_01145"
     CDS             complement(4820..>6769)
                     /gene="pbpC"
                     /locus_tag="VGC27_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEY0281210.1"
                     /translation="GVLLRAFLSRGGYWRMKTGVSEVSPRYLAMLKAYEDKRFDGHFG
                     VDPLAMARAALQFASAGRIVSGGSTLTMQVARLLEPPKSRGIGTKLIQMVRAVQLEER
                     YSKDEILSFYLTLAPMGGNLEGARAASLSYFGKQANQIDLAEAALLVALPQSPGKQRP
                     DRHAIAAAKGRDKVLMRMVREGAVSEADAKLAMKEGVPFARQAMPLAAPHLAERLARS
                     MKSTHIVTTLDAGLQGAVERLAVQESGYFGDGAALAVVVVENKTRNVLAYLGGTDYWG
                     KAGQIDLARQARSPGSALKPFIYGLAFDEFILHPSTMMQDAPISFGDYAPRDFDGGFQ
                     GAVTARDALRMSLNVPAVMVLDRVGPLAFTLALQNAGARLAFPSREEGPSLPVALGGL
                     GISPADITMLYAGIAEGGQARALRYVEGAPDAPRHRLFGAAAAYYLRDILDGVTLPEG
                     WAMGAGLARQRSIGFKTGTSYGFRDAWSVGFSNDYTVGVWVGRADGTPRPGHVGRDAA
                     APILLKVFGLLPPDRRPAPERPAGVILAQSTDQLPPSLRLFTRAEQPQMAQQTVPPPA
                     ISFPPNGTVVPLPPASAKDKTIMLKADGGRAPLTWLVNGALLGSFDRFQPALYLPRGE
                     GLARITVVDSEGRSDSSEVRFKRDR"
BASE COUNT         1182 a         2254 c         2185 g         1148 t
ORIGIN      
        1 atcctggtcg acatgcaagg cccgaaaatc cgccttggaa ccctggccgg cggcgtgcgc
       61 gaggttcaca acggcgaccg tctgcgtctg gtgcgcaaag tcgcggccga aaatgaaacc
      121 gacatcccca tcccccatgc cgaggttttc gccgcgctgc ggcccggcga gaatatcctg
      181 atcgacgacg gcaaggtccg cctcaagatc gaagaagtga ataaggattg ggccaatgcc
      241 gtggttgaag cgggcggcct gttgaaggac aaaaagggcg tcaatctgcc cgacacgata
      301 ttgccgatcc cggccatgac gcccaaggac cgcagcgatg tcgatgccgc gctgcatctg
      361 ggcgtggatt ggatcgcgct cagcttcgtg cagcgccccg acgacgtggc cgaattgaag
      421 aaggtggtcg gcgggcgcgc cgcctgcctc gccaagatcg agaagcccag ggccatcgaa
      481 tggctggaag acatcctcga catctccgac gcgctgatgg tggcgcgcgg cgatctgggc
      541 gtcgaactgc cggtccagga agtgccgggc aagcagaaat tcatcacccg ggcggcgcgc
      601 aaggcgggca agccggtcgt ggtggccacc cagatgctgg aatcgatgat cacggccccg
      661 gtgccgaccc gcgccgaagt ctccgacgtg gcgaccgccg tgttcgaggg cgccgacgcg
      721 gtgatgctgt cggccgaatc cgccgccgga aaatatccgg cgcaagccgt cgccatgatg
      781 gacaagatcg cgaccacggt ggagggcgat ccgctctacg tttccatcat cgatgcgcag
      841 cgcttcgcgc ccgaggcgac cacggccgac gcgatcatgg ccgcggtgca tgaagtcacc
      901 cacacgatcc acgcccgcgc catcgtctgc tggacgaaat ccggcgccac cgcgttgcgc
      961 gccgcgcgcg agcgctccga cgcgccgatc atcgcgctga cgccgagtct ggagatttcg
     1021 cggcagctct ctctcgtctg gggcctgcat tcggtctaca ccgaagacgc gcaggacctc
     1081 gacgacatgg tcgaacgcgc cgcgcgcttc gccgcccagg aaggcttcgc caagtccggc
     1141 gaccgcatcg tggtgacggc cggcgttcct ctgcgcacgc cgggcgccac caacatgctg
     1201 cgcgtggcgt tcgtgggatc gcaaaaaaac gaatagcgaa tagggatttc cctactcgct
     1261 attcgctact ccctattcgc tacttctata tcccctgccc ctccaccgcg cgcgaaagcg
     1321 cgtcgacgtg gcgctgcaag ccctcgaact gcggattgag cgccagcgcg cggcgataca
     1381 ttctgagcgc gccttccttg tcgccatagg cttccagcat ctcgcccaac tgttccatcg
     1441 cctcgaactg gcgcggattg agcgcgatgg tcttctgcag cgacaccatc gcgccttcgt
     1501 cgtcgccggt ctcagcttgc agcagggcgc ggcggtgcca gccttcggcg tagttcgcag
     1561 cgatattcgt caccgcatcg agaagctggc gcgccgtgtc cctgtcgccc gccccctggg
     1621 cggccgccgc gcgcgccatc agcagatcga tgctcgggct tcccgattgg cggaaatgcg
     1681 ccaggatctg ctcttcgatg ggtttggcgt cttccggcgt ccccgcggcg cgaagctgcg
     1741 cgaaaagggc gtcgagcggg ctgggcggcg cggcccaggc cgccgccggg agcaacaagg
     1801 caagcaggaa gatggcgaga cgcatgcccc aaatctacga tggtttgggt tcggcttaaa
     1861 tcacctctgt catggcccgc acgcgggcca tgacaccggg gaatgtgtct aaaccttaac
     1921 cttggcgcgc cttgaaacgg gggttcttct tgttgatgac ataggtgcgg ccgcggcggc
     1981 ggataacgcg gcagtccttg tcgcgctttt tgagcgagcg gagcgaattg cggactttca
     2041 tggcacgcct tttgaactta tttcctgcga gatggccccg ccaaagaggc gcggaagcta
     2101 gtcggcgggg atttgtgtgt caacagcgcg aaaatctggg atttctgccg caatttctac
     2161 aggggccgcg tgctgaaaac tgccccgaaa cgggccgttt gagtaggggg agctaaattc
     2221 ggtgcgtctg atattactat tcctggccta tatcctgtcc ttgatcatca tggctgcggc
     2281 atacggggcg atcgccgccg aggcgccgcc tgtctccgcg ccgcttgccg cggccgccgt
     2341 gcccgtcccg gccccggtgc cggacgaaaa cctcgccttc gcccggttcg tgcgcgattt
     2401 ccgcgccacc gccttggccg ccggcatcgc ccctgaaatc tacgacgcgg cgatgagcag
     2461 gatcgcccgt aaccagcgcg tggtggatct caacctcaac cagcccgaat tcaccgctcc
     2521 ggtctggtcc tatctggaca acgcggtctc gccccagcgc atcgccggcg gccgcgccgt
     2581 actggcgggc aacgccgcca ttctggcccc catcgtggcg aaataccgcg tgcccaagga
     2641 gatcctggtt tcgatctggg gcaacgaatc cgactacggc cgcagcatgg gccgcttcaa
     2701 cctgttcgag gcgctggcga cgctggcgta tgacgggccg cgcatcgact atgcccgccc
     2761 gcaactgatc gcggcgttgc agatgatgca gcggcagaaa ttcgccgccg ccgacatgat
     2821 gtcgtcctgg gcgggcgcct tcggccagac ccagttcgtg cccacgacct ttctcgccca
     2881 ggccgtggac ggcgacggcg acggcaaggt cgatttgtgg aacagcgtcg ccgatgcgct
     2941 cgcctccgcc gccagggtga tttccgacgc cggctggcga gaaggcaagc cgtggggcta
     3001 tgaggtcgtc ctgccggggg ggttcgctta cgaagacgcc gatctcgaca acgcgctgcc
     3061 gctcgccgtg tggaagaagc atggcgtcaa gacggcggcg ggcgggactt tgccggcgag
     3121 cgacgagccg gcctcgatct atctgccggc gggcgcgcgc gggcctgcgt tcttcgtttt
     3181 ttccaatttc aagaccattc tgaaatacaa caacgccgca agctatgcgc tggcggtgtg
     3241 cctgttggcc gatcagttgc agggcatcgc gcccgtcgcc gcatcctggc cgcgcgatca
     3301 ggcggctttg aaccgcaacg aacgcttggc gctgcaaacc tcgctggcca aactcggcca
     3361 cgacatcggc aagatcgacg gcttcatcgg cagcaaatcg cgcgccgcca tccgggcctg
     3421 gcagaagaag cacggcctgc ccgccgacgg cttcgccacc ctcgacctgt tcacccgcat
     3481 cgcgatggac gcaaaggggc aataatccgc aattccgagc acgccggaga tgggcgcgcc
     3541 cgcatctccc gcatctgaaa aagacgccct cgccccagcg acgggacaaa ggcgttcaag
     3601 gcaagcatgc aggaaggatc ctgtattcag tgcgttgcgt ccgacacttt gtcgttcggc
     3661 gggatcaggg cgtgcaggat cggctcgacc cattggtcct tgtcgatgtc gtagacgatg
     3721 aaatccttgt cgaactgcca ataggcccaa gagaagccgc gcgcttcggc gccgcggacg
     3781 accgcggagg tccacctcac ccggtcggcc gtcgccccgt tttcataggc gccgaattcg
     3841 cccaggaaaa tcggccggtt gtgcgcctcc gaccacgcct tcacgatgtc gaattccttg
     3901 ttcagcagcg caaagtccgc gtccgagccc caggtgacgc caagctgctc gtactggggc
     3961 gtccaggccg cgccctgatg ggtgaattcc atcgggtgat aataatgcac ggtggcgatg
     4021 atgtgctgat cgtccgccgg caattcgagc ttgggcagaa gttcgaggcc gttccaaaag
     4081 cccggcccga tgacgacgtt gcgcgtcgga ttggtcgccc ggatgatggc gagcgtttcc
     4141 accagttgct cgttccacaa ttccggcgtc aaggcctcgt gcggctcgtt gagcatttcg
     4201 aacacgagct tgttcggcgc atccttgtag cgcggagcga cctgcgacca gaacgcattc
     4261 agcttggctt tgcagacgac gacatccttg ccgcagagtt cgtagtcatg ctcgtcgatg
     4321 acgacgttca gatcctgagc gagcgccgcc ttcaccatga gatcgagccg ctccagatag
     4381 cgcggatcga gcgtgtaggc cgcgtccatg tgatcgaagg ccgtcagaac gatgcgcacc
     4441 gtgtcgaagc ccgcgtcgcg aatgagcttg aaatgccggg gcgtgaagcg ccccttggcc
     4501 ggatcgttcc agcccgggtc ctcgctcagc accgctacgc cacggcccat ctgggtattc
     4561 tgcgcgaatg catcgatggg cacataaggc tgttcgggga acaccgcttc cttcggctgc
     4621 tccggcgaga tgctcgcgca gcccgccagc gccgccgcca gaacaacgcc aacaaccgcg
     4681 ccgatgcgcg aagcgctgga aaatggtctc atctcttgcc tccctggaaa cggcgggatc
     4741 gccacaccgc ttctcgctct tttgaccgat aatgatagcg cttaccgcgg ccgtcaagaa
     4801 acggcacggt gacggccctt cagcgatccc gcttgaagcg cacttcggaa ctgtcgctgc
     4861 ggccttcgga atccaccacc gtgatgcgcg caagcccttc gcctctcgga agatagagcg
     4921 caggttggaa acggtcgaaa ctgccgagca gggcgccgtt gaccaaccag gtgagcggcg
     4981 cccgcccgcc gtcggccttc agcatgatgg tcttgtcctt ggcgctggca ggcggcagag
     5041 gtaccactgt gccgttgggc ggaaatgaaa tggcgggcgg cggcacggtc tgctgcgcca
     5101 tctgcggctg ctcggcgcgc gtgaagaggc gcagcgacgg cggaagctgg tcggtgcttt
     5161 gcgccaggat gacgcctgcc ggccgctccg gcgcgggccg tctgtccggc ggcaacaggc
     5221 cgaacacttt gagcaggatg ggcgcggccg cgtcgcgccc gacatggccg gggcgcgggg
     5281 taccgtcggc gcggccgacc cagacgccca ccgtgtaatc gttggaaaac cccaccgacc
     5341 aggcatcgcg gaagccgtaa gaggttccgg tcttgaaacc gatgctgcgc tggcgcgcca
     5401 gcccggcgcc catcgcccag ccttcgggca gcgtcacgcc gtcgaggata tcccgcagat
     5461 aataagccgc cgccgcaccg aacagccggt gccgcggtgc gtcgggcgcg ccttccacat
     5521 aacgcagagc gcgcgcctgc ccgccttcgg cgatccccgc atagagcatg gtgatgtcgg
     5581 cgggcgaaat acccagcccg cccagcgcca ccggcaggct cggcccttct tcgcgcgagg
     5641 ggaacgccag gcgcgcgccc gcgttctgca aggcgagggt gaaggcaagc gggccgacgc
     5701 ggtccagcac catcaccgcg ggcacgttga gcgacatgcg cagcgcgtcg cgcgccgtca
     5761 ccgcgccctg gaagccgccg tcgaaatcgc gcggcgcgta atcgccgaac gagatcggcg
     5821 cgtcttgcat catggtggag ggatgcagga tgaactcgtc gaaggcgagg ccgtagatga
     5881 acggcttgag cgcggaaccg ggcgagcgcg cctgccgcgc cagatcgatc tgcccggcct
     5941 tgccccagta atccgtgccg cccagatagg cgagcacgtt gcgggtcttg ttctcgacca
     6001 cgacgacggc cagcgcggcg ccgtcgccga aatagccgct ttcctgcacg gccaggcgct
     6061 ccaccgcgcc ttgcaggccg gcgtcgagcg tggtgacgat gtgggtggat ttcatgctgc
     6121 gcgccagacg ctcggccaga tgcggcgccg caagcggcat cgcctggcgc gcgaacggca
     6181 cgccttcctt cattgccaat ttggcgtcgg cttcgctgac cgcgccttcc cgcaccatgc
     6241 gcatcagcac cttgtcgcgg cctttggcgg cggcgatggc gtggcggtcc gggcgctgct
     6301 tccccggcga ctgcggcagc gccaccaaga gcgccgcctc ggccagatcg atctggttcg
     6361 cctgcttgcc gaaataggac agcgaggccg cgcgcgcgcc ttccaggttg ccgcccatcg
     6421 gcgccagggt cagataaaag gaaaggattt cgtctttgga atagcgctcc tcaagctgca
     6481 ccgcgcgcac catctggatc agtttggtgc caatgccgcg cgacttgggc ggttcgagca
     6541 gccgcgccac ctgcatggtc agggtcgaac cgccggaaac gatgcgcccg gccgaggcga
     6601 attgcagcgc cgcgcgcgcc atcgccagcg gatcgacgcc gaaatgcccg tcgaagcgct
     6661 tgtcttcata ggctttcaac atggcgagat agcgcgggct gacctcgctg acgccggtct
     6721 tcatgcgcca atagccgccg cgcgaaagaa aggcgcgcag cagcacgcc
//
LOCUS       DASWPU010000056        10904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_92138,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000056 DASWPU010000000
VERSION     DASWPU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92138"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..327
                     /locus_tag="VGC27_01150"
     CDS             100..327
                     /locus_tag="VGC27_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY0281211.1"
                     /translation="MVTSIRLEAPLEKRLDELARRTGRTKAYYLRELVQRGLDDLEDY
                     YLAAETVERIRKGEEQTYSAADVRSDLGLDD"
     gene            311..580
                     /locus_tag="VGC27_01155"
     CDS             311..580
                     /locus_tag="VGC27_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0281212.1"
                     /translation="MAWTISYTDTARKQLRKLDKQTARRILDFMDERIAGAHNPRDAG
                     KALTGPMLGTFWRYRVGDYRIICEIQDGKLCVLVVEIGHRREVYR"
     gene            596..736
                     /locus_tag="VGC27_01160"
                     /pseudo
     CDS             596..736
                     /locus_tag="VGC27_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            836..1252
                     /locus_tag="VGC27_01165"
     CDS             836..1252
                     /locus_tag="VGC27_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HEY0281213.1"
                     /translation="MMKPVLAPLLALGIVLGFAAIPARAATAEPTSSAWQWLALIDQE
                     QYVESWAEGSALFRARITERSWESKTSLYREAVGRAVWRDVDSVSLAGRLPDLPEGQY
                     ALVRYRSKFERKTDCTETVGLVLENGLWRVASYSIK"
     gene            1406..2098
                     /locus_tag="VGC27_01170"
     CDS             1406..2098
                     /locus_tag="VGC27_01170"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEY0281214.1"
                     /translation="MRKMFMAATAVALVFGATAAPAQRGATPPPDRDPDGYYSQSDQD
                     GYYDRDGNYVRYDNRNQDYGYNGPDRRDTNYGPPPDTYRQGVYEDNCRRSNNAAGTIF
                     GAIAGGLIGSAASHGRSHHGHGNNQGGAIVGGAILGGLLGNALTRDIPCEDHRHAFSV
                     YAQGLEGPVGGRYDWRNRDNGDYGYFIPQREFKRSGYVCRSFSTTTYRGGRTYTQSGT
                     ACRMSDGNWRFD"
     gene            2303..2887
                     /locus_tag="VGC27_01175"
     CDS             2303..2887
                     /locus_tag="VGC27_01175"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HEY0281215.1"
                     /translation="MPETWTISANGHTYGPYTAAQMRAFAAEGRLAAHSLVAREGEEQ
                     FHPASADSELAALFRPAAPAPAPARPVFYTADGETPQQSFGRNEDDKTGERSHYVIVA
                     DMKSRSISGLEEEIFNFGPAYPIMPQGWILSSEMSINAIRNTLVKKLGKLDTLFVVDA
                     THDKAAWFNFGPEAETRFRRIWQKAAEPAVRAAG"
     gene            complement(2888..3418)
                     /locus_tag="VGC27_01180"
     CDS             complement(2888..3418)
                     /locus_tag="VGC27_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY0281216.1"
                     /translation="MPVYALNGVAPELPADGQYWIAPTAVVVGRVRLMKNASIWFGAV
                     LRGDNDLISIGESSNIQDNAVVHTDPGQPATVGANVTVGHHVILHSASVGDNSLIGMG
                     ATLLNRSRIGKHCIVGANALVTEGKEFADGMLIVGAPARAMRPLAETELALLKASAEI
                     YVANYRRFRDGLGVPA"
     gene            3690..4436
                     /locus_tag="VGC27_01185"
     CDS             3690..4436
                     /locus_tag="VGC27_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY0281217.1"
                     /translation="MAKRSARYKSRESSFADDENVRPLFPAKGGWTPRDGEARDRKYV
                     KNVRAMSPTQQRFMDAIDERAVALALGPAGTGKTYLAIAKAVEALETGRASRIVLSRP
                     AVEAGESLGFLPGDLQDKLAPYLRPLYDCLTERLGTKRLKALVAEGVIEIAPVAYMRG
                     RTLNDCFIVIDEAQNCTYGQLKMLLTRLGWRSTMVLTGDPDQTDLLSGLSGLHDVAER
                     LEKVPEIAVIWLGEGDIVRHPLVAGMLTVL"
     gene            complement(4554..4871)
                     /locus_tag="VGC27_01190"
     CDS             complement(4554..4871)
                     /locus_tag="VGC27_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281218.1"
                     /translation="MPKHTGVGMLRDIIVILFSVAAGFTASGIVANLYRLMADKPQSR
                     AGKTFYIAVMVIAGPCVLVANAAKSRRAKGCSHFAFWLATAVAGYWSFAIGLFVLDLS
                     LAL"
     gene            5066..5797
                     /locus_tag="VGC27_01195"
     CDS             5066..5797
                     /locus_tag="VGC27_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281219.1"
                     /translation="MSRTTDDDETIRKHSGWLIPLGVFLVTFVLSALFLLIYLVPSAP
                     SLFQEQVSPTSRSDVIALKVGGRAFHIPANYLEYESARRGGDRREVALYALLPDMSGW
                     SNWDAQSFTDNSANSKVLEMRLRQDNLNLSEADRLQRVYMGYIANPRGTEGPYGLKQY
                     AFRADSGYHNEDLLVGETETGLLVMRCVRLGPDVTSPNCMRDMPVARGVSLFYRFKRA
                     HLSQWRKIDAGVTKLIASFEKAPPK"
     gene            5872..6342
                     /locus_tag="VGC27_01200"
     CDS             5872..6342
                     /locus_tag="VGC27_01200"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY0281220.1"
                     /translation="MAGKLAFLFVCLGNICRSPLAEAAFKAEAARIGLDAEVDSAGIG
                     GWHAGEPPDPRARAVAKRHGMDISLYRARKVTQADFRRFSHVVALDHEVLAALKAMRP
                     ANAGAKISLLLDHVEGRKDEEVADPYYGDEAGFETAWADIADGVKALARRAAKK"
     gene            6339..7145
                     /locus_tag="VGC27_01205"
     CDS             6339..7145
                     /locus_tag="VGC27_01205"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEY0281221.1"
                     /translation="MNALAEAGAALIGGVLRRSEALAGGSLSQIARIELTDGRDAIVK
                     NGPAPRIEAAMLQAIAASAAPAPAVLGVSDEVLVLERLPAGGALHHAWPSLGTALATL
                     HQAKGERYGWPENYAFASVEIENGWADDWPRFWAERRLLVHVAHLPPSLARRLEALAA
                     DLPNRLPARPKPSLLHGDMWGGNVLVAENRVSGLIDPACIYGHGEVDLAMLTLFDGPS
                     AAFYDAYGPLQPGHEERLAIYQLWPALVHLRLFGSGYRGMVERFLADAGV"
     gene            complement(7324..8562)
                     /locus_tag="VGC27_01210"
     CDS             complement(7324..8562)
                     /locus_tag="VGC27_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281222.1"
                     /translation="MAGNGGEAGAARQFALRNRVLLLSLSLSVVGIATGVATANSILA
                     KASLGNAGLILAGAALAAVVSLLHTRHLALALLTAAAPLPGLIWAAPVSGDASYGAVP
                     VLAYIFAYAIAVMLAHNILIRTLGGGTAEHPFKPAAAAAGLMTALSILWFWRTRAGAA
                     SFQAVMDVVLATASTLIIMPIGEWFLHFDEMFVATANRVRERRQRLFEKIAMTAVPRW
                     GMSLAGIALIFVALAWFGAEPAFSFVPLVNAKVLLAVSLGLALALFMPVCPGWREGIG
                     ATVAAGLAGLMALWAFAAIGKMTPAAPAGIVEIISLTLFLVFCGTRRAAAYRRLGDDP
                     AIARLRAVEDTGGPLLFAILGGIAALLPFIAVYPAHAAYAVALAFAAAGALGFAPALA
                     TACEMLVPRRRSFKELYGRR"
     gene            complement(8569..8802)
                     /locus_tag="VGC27_01215"
     CDS             complement(8569..8802)
                     /locus_tag="VGC27_01215"
                     /inference="COORDINATES: protein motif:HMM:NF022768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HEY0281223.1"
                     /translation="MTRSEIWRIEKYLRNLFRLDTISVVERPKQADSVEVHVNGEFIG
                     VIFKDEEDGETSYAFNMAILEMDLPEPPASRAG"
     gene            complement(9099..9914)
                     /gene="cysE"
                     /locus_tag="VGC27_01220"
     CDS             complement(9099..9914)
                     /gene="cysE"
                     /locus_tag="VGC27_01220"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY0281224.1"
                     /translation="MAVTVEKVASCDPIWSALREQAQSLALREPTLTSFVHATILKHN
                     RLEDALSYHLAKKIGGEDLSPMLTREIFEEAIDADPSIVEAVRADLSAVFDRDPACHS
                     HAEAFLFFKGFQALESYRIAHWLWTQKRDGMALFFQSRISQLFDVDIHPAAKLGRGIM
                     IDHATGVVIGETAVVEDDVSMLHGVTLGGTGKETGDRHPKVRRGVLLSMGVKVLGNIE
                     IGEYSRVGAGSVVLKSIPPHCTAVGVPARLINCAGSERPSQEMDQFIDEADSI"
     gene            10042..10644
                     /locus_tag="VGC27_01225"
     CDS             10042..10644
                     /locus_tag="VGC27_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY0281225.1"
                     /translation="MNSRPKDIFNKAAPQTIDLLLSRRSGTAKAMTGPGPDAEELRTI
                     LRAAARVPDHGRLFPWRFIVFEGDARARFGKLLVECLKETDAVIVTPERAAQEAGRFL
                     RAPVVVGVVSRVRAAIPIPEWEQQLSAGAVCQTVLIAAHALGFVANWLTEWYAYHPLV
                     SERLGLKPGERIAGFIYIGKSAIALEDRLRPDMEKIVTRF"
BASE COUNT         2141 a         3553 c         3324 g         1886 t
ORIGIN      
        1 ggggcacctg tccctaactc ccgcctggac gcctgtcatt gacataatgc cgtccagtga
       61 atacattgaa tacacgtatt cagcagcaga gttttcccca tggttacctc catccgcctc
      121 gaagcccctc tggaaaaacg cctcgacgag ttggcccggc gtaccggccg gaccaaagcc
      181 tattacctgc gcgaactggt tcagagggga ttggacgatc tggaggacta ctacctcgcc
      241 gctgaaacgg tcgaacgcat ccgcaaggga gaagaacaga cctattccgc ggcagatgtg
      301 agaagcgacc ttggcctgga cgattagcta caccgacacg gccaggaagc agcttcgcaa
      361 actcgacaag cagacggccc gccgcatcct tgatttcatg gacgaacgca ttgccggtgc
      421 gcacaacccc cgcgacgcgg gcaaggcgct caccgggccg atgttaggca ccttctggcg
      481 ttaccgggtt ggggactacc gtatcatctg cgagattcag gacggtaagc tgtgtgtgct
      541 cgtggtcgaa atcggccacc gccgggaagt ctataggtag cgctatccca accgcgacga
      601 cgccaactgg atgaagcaca tcttgtcgtg gttcgacgcc aagaccggcg aagtccgcct
      661 cgactaccgg ccggtccaca cctacacgct gacctacgaa gtggaataca tcccgccgaa
      721 agccagggtg tactgacggc ccctcccgct ctttcctaaa aatagggggg ccggccgggg
      781 gactgtatcg gaaccgtcat gattggcggc caaagtccct gtgcagaggg acgccatgat
      841 gaaacccgtt ctcgctcccc ttttggcgct cggcatcgtc ttggggtttg ccgccattcc
      901 ggcccgggcc gccaccgcgg agccgacctc cagcgcctgg cagtggctgg cgctgatcga
      961 ccaggagcaa tacgtcgaga gttgggctga gggcagcgcc cttttccgcg cccgcatcac
     1021 cgaaaggtcc tgggaaagca agacgagcct gtaccgcgag gccgtcggcc gcgccgtctg
     1081 gcgcgatgtg gatagcgttt cgctcgccgg ccgcttgccc gacctgcccg aaggccaata
     1141 tgcgctcgtg cgctaccgct ccaaattcga gcgcaagacg gactgcacgg agacggtcgg
     1201 cctggtcctc gaaaacggct tgtggcgcgt ggcaagctat tccatcaaat aggcaacccg
     1261 gcctgctgca gatcgtggcc cggaagcggt aagcgcagcc aaaatcccgc cttattccgt
     1321 tcagcgggca ttcagcctgt atgagtcatg ctatccttac ggtttgcctg tctgcccacg
     1381 gcaagcgaca aaggagagca gcgtgatgag gaagatgttc atggcggcga cggcggttgc
     1441 gctggtgttc ggcgcgacgg cggcgcctgc gcaacggggc gccacaccgc cgcccgaccg
     1501 cgaccctgac ggctactaca gccaaagcga tcaagacggc tattatgacc gcgatggcaa
     1561 ctatgtccgt tacgacaacc gaaaccagga ttacggctac aacggccccg accggcgaga
     1621 tacaaactac ggcccgccgc cggacactta tcgacaaggc gtttacgaag ataattgccg
     1681 ccgcagcaac aatgccgccg gaacgatttt cggcgccatc gcaggcggcc tgatcggatc
     1741 ggcggcgagt cacggccgca gccatcacgg tcacggcaac aatcagggcg gagcgatcgt
     1801 cggcggagcg atcctcggcg gcctgctcgg caacgccctg acccgcgaca taccttgcga
     1861 agatcaccgc catgccttca gcgtttatgc gcaaggcttg gaaggcccgg tcggcgggcg
     1921 ctacgattgg cgcaaccggg acaatggcga ttacggctat ttcattccgc agcgcgaatt
     1981 caagcgtagc ggttacgtct gccgttcgtt cagcaccacg acctaccggg gcggacgcac
     2041 ctacactcaa tccggtacgg cctgccgcat gagcgacggc aactggcgct tcgactgaac
     2101 ggcgctccag cggccatgat aaaaccccgg cttggcggca ggccggggtt ttcttttggg
     2161 tgtgcgcctt ccgccggaaa cgcgtctttc ccctggattt gtcccccgcc gccgcaagag
     2221 ccttacgtgc cgctaccgga cggaaagaat tgagtaacca ttcggcccta ggctcgaagc
     2281 agtcactgga ttttgagccg aaatgccgga aacctggact atcagcgcga acggacacac
     2341 ctacggcccg tacacggcgg cgcaaatgcg cgccttcgcg gcggaaggaa ggcttgcggc
     2401 ccattcgctc gtcgcccgcg aaggcgagga gcagttccat cccgcctcgg ccgacagcga
     2461 actggccgcc ctgttccggc ccgcggcgcc cgctcccgcg ccggcaagac ccgtctttta
     2521 caccgccgac ggcgagacgc cgcagcagag tttcggccgc aacgaggacg acaagacggg
     2581 cgaacgttcg cattacgtca tcgtcgccga catgaagtcg cgttcgatct ccgggctgga
     2641 agaagagata ttcaatttcg ggccggccta tccgatcatg ccccagggct ggatcctgtc
     2701 cagcgagatg tcgatcaacg cgatccgcaa tacgctggtg aagaaactcg gcaagctcga
     2761 cacgctgttc gtcgtcgacg ccacgcacga caaggccgct tggttcaatt tcgggcccga
     2821 agccgagacc cgcttccgcc gcatctggca gaaggctgcg gagccggccg tccgcgcggc
     2881 cggctgatca ggccggcacg ccaaggccgt cgcggaagcg ccggtaattc gcgacgtaaa
     2941 tctcggccga cgctttcagc aacgccagct ccgtttcggc gagcggccgc atcgcgcgcg
     3001 ccggagcgcc gacgatcaac atgccgtcgg caaactcctt gccctcggtg acgagcgcat
     3061 tcgcgccgac gatgcaatgc ttgccgatcc gcgaacggtt gagcagcgtc gctcccatgc
     3121 cgatcagcga attgtcgccg acgcttgccg aatgcaggat cacatgatgc cctacggtca
     3181 cgttcgcgcc caccgtcgcc ggctggccgg gatcggtatg aaccacagca ttatcctgga
     3241 tattgctgct ctcgccgatc gagatcagat cgttgtcgcc gcgcagcacg gctccgaacc
     3301 agatgctggc gttcttcata agccgcacgc ggccgacgac aaccgccgtc ggcgcaatcc
     3361 agtactggcc gtccgccgga agctccgggg caacgccatt caacgcatag accggcatcc
     3421 gaaatcccgc ctgcttaacc gcttcgcttg acattctgac gacgatttga cgattgcgcc
     3481 agttcgccct tcgccgcgaa aaaatgcggc tgcgattttc gaagttctgt tattctgaat
     3541 gcgttcgcgt gattctttca cgccaggggg tttacggtgc gtcgctgcac tttgcgaatt
     3601 ccgaatttcc aaggcgccat gccccgtccc cgcgcacatt cttctttgct tcttccgatc
     3661 agcgttcgtt ttttgaccag ggagtttcca tggccaagcg ttccgcgcgt tacaaatccc
     3721 gtgaaagcag ctttgcagac gacgaaaacg tccgtccatt gttcccggca aagggtggat
     3781 ggactccgcg cgacggagag gcgcgcgacc gcaaatacgt aaagaacgtc cgggccatga
     3841 gcccgaccca gcaacgcttc atggacgcca tcgacgagcg tgccgtggcg ttggcactcg
     3901 gtcccgccgg aaccggcaag acctatctcg ccatcgccaa agccgtcgaa gcgctggaga
     3961 ccggccgtgc cagccgcatc gtgctgtcgc gcccggcggt ggaggccggc gagagcctgg
     4021 gcttcctccc cggcgacctt caggacaagc tggcgcctta cctgcggccg ctctacgact
     4081 gcctgaccga acggctcggg accaaacggc tcaaggcgct cgtggccgag ggcgtgatcg
     4141 aaatcgcgcc ggtcgcctat atgcgcgggc gcacgctcaa cgactgcttc atcgtcatcg
     4201 acgaagcgca gaactgcacc tacggccagc tcaagatgct gctgacccgg ctcggctggc
     4261 gctcgaccat ggtgctgacg ggggatccgg accagaccga cctgttgagc ggcctgtcgg
     4321 gtctgcacga cgtggccgag cggctggaga aagtgcccga gatcgcggtc atctggctgg
     4381 gtgaaggcga catcgtgcgc catcccctgg tcgccggaat gctgaccgtt ctctaggaca
     4441 cgatgagccc cacccttcga caggctcagg gtgaggggca gcgtttctcc ttcatgctga
     4501 gcttgccgaa ggatgaggtg cgcaatcgcg aatagaccga ggattcaggg cagttacaaa
     4561 gccaagctga ggtcgaggac aaagagcccg atggcgaagc tccaatagcc ggcgaccgcg
     4621 gtcgccagcc agaaggcgaa atgggagcag cccttggccc ggcggctctt ggccgcgttc
     4681 gcaaccagaa cgcaggggcc ggcgatgacc atgacggcga tataaaaggt ctttccggcc
     4741 ctgctttggg gcttgtccgc catcaggcgg tacaggtttg ccacgatccc tgatgccgtg
     4801 aacccggcgg cgaccgaaaa caatatgacg ataatgtcgc gcaacatgcc caccccggtg
     4861 tgcttgggca tggtgttgca ggcgctgtgc ccgtcccgga ggcgtgcccg gagctgtgtt
     4921 ttgtgccgtt aagggacggt tccagggcgt atccccggcc gccgcttgag gcggccgtgt
     4981 cgatttggac cgcaaccggt gcagaacgtt aattccgtta tgctagatag agcggttacg
     5041 ataccgactc ggatacggac ggggaatgag ccgcacgacc gacgatgatg aaacaatccg
     5101 gaaacactcc gggtggctga tcccgctcgg cgtgttcctg gtgacgtttg tcttgtcggc
     5161 gctgttcctg ctgatctact tggtccccag cgcgcccagc ctgtttcaag agcaggtttc
     5221 gccgacttcg cgcagcgatg ttatcgccct caaggtcggc ggccgcgcct ttcatattcc
     5281 ggcgaactat cttgaatatg aaagcgcgcg ccggggcggc gaccgccgcg aagttgcgtt
     5341 gtacgccctg ctcccggaca tgtccggctg gtccaactgg gacgcgcagt cctttaccga
     5401 caacagcgcg aactcgaagg tcctcgaaat gcgtctcagg caggataatc tcaacctgag
     5461 cgaagccgac cggctccagc gggtctatat gggctatatc gccaatccgc gcggcacgga
     5521 aggaccgtac ggcctgaagc aatacgcctt ccgcgccgat tccggctatc acaacgagga
     5581 tctgctggtg ggcgaaaccg aaaccgggct tctggtgatg cgctgcgtgc gcctgggacc
     5641 ggacgtgacc agtccgaact gcatgcgcga catgccggtg gcgcgcggcg tttcgctgtt
     5701 ctaccgcttc aagcgcgcgc acctttccca atggcgcaaa atcgacgccg gcgtgaccaa
     5761 gctgatcgct tcgttcgaga aagcgccgcc aaaataattc aggaccagat tggaaattca
     5821 cagccgccgc gtttcgagcg aaaatcgcct tgatccaggg agtttcaact cgtggccgga
     5881 aagcttgctt tcttgttcgt ctgtctcggc aacatctgcc gttcgccgct cgcagaagcg
     5941 gccttcaagg cggaggcagc gcgaatcggc ctcgacgcgg aagtcgattc ggccggcatc
     6001 ggcggctggc atgccggcga accacccgac ccgcgcgccc gcgccgtcgc caagcgccac
     6061 ggcatggaca tttccctcta tcgcgcacgc aaagtgacgc aagcggattt tcgccgcttc
     6121 agccatgttg tggcgctcga ccatgaggtg ctcgccgcgc tcaaggcgat gcgtccggcg
     6181 aatgccggcg cgaagatcag cctgctgctc gatcatgtcg aggggcgcaa ggacgaggag
     6241 gtcgccgatc cctattatgg cgacgaggcc ggcttcgaga ccgcctgggc cgacatcgcg
     6301 gacggcgtga aggcgctcgc acgccgggcg gcaaagaaat gaatgcgctc gccgaggccg
     6361 gcgccgcgtt gatcggcggc gtccttcgcc ggagcgaagc gctcgcgggc gggagcctgt
     6421 cccagatcgc gcggatcgaa ctgaccgacg ggcgcgacgc gatcgtgaag aacggacccg
     6481 cgccaaggat cgaagcggcg atgctgcaag ccatcgcggc gagcgccgcg cccgcccctg
     6541 ccgtgctcgg cgtcagcgac gaagtgctgg tgctcgaacg tctcccggcc ggaggcgcgc
     6601 tgcaccatgc ctggcccagc ctgggaacgg cgctcgccac gctgcatcag gcgaaaggcg
     6661 agcgctacgg ctggccggag aattatgcct tcgcatcggt cgagatcgaa aatggctggg
     6721 cggacgactg gccgcgcttc tgggccgagc gccgcctgct ggtccatgtt gcgcatcttc
     6781 cgccgtctct cgcgcgccgt cttgaagcgc ttgccgccga tcttcccaac cggctgccgg
     6841 cgcgcccaaa gccttccctg cttcacggcg atatgtgggg cggcaatgtg ctggtggcgg
     6901 aaaaccgcgt cagcggcctg atcgatccag cctgcattta cggccatggc gaagtcgatc
     6961 tcgcgatgct gacgctgttc gatggaccct ccgccgcatt ttacgacgct tacgggccgt
     7021 tgcagccggg ccatgaagaa cgcctggcga tctaccaact gtggccggcc ctcgtccact
     7081 tgcgtctgtt cggcagcggt taccggggaa tggtcgaacg cttcctggcg gacgccgggg
     7141 tttgaccgcg cggaagaacg cacactccgg cttcatcatg gccgggcttc atcctgagga
     7201 gccgccctcg tccttcgaga cggccccctt cgggtgcctc ctcaggatga gggcggcggc
     7261 tcaaaggccg cccccatggt gaggagtgtg gaaacgagcg cctcgattta ttccaaagcg
     7321 actctaccgc cgtccgtaca gctctttgaa ggaacgccgt ctgggcacaa gcatctcgca
     7381 ggccgtcgcc aacgccggag caaagccgag cgcccccgcc gccgcaaacg ccagcgccac
     7441 cgcataagcc gcatgggccg gatagacggc aatgaacggc aaaagcgcgg cgattccgcc
     7501 caggatcgcg aacagcagcg gaccgcccgt atcttcaacg gcacgcaacc gggcaatagc
     7561 cggatcgtcg ccaaggcggc gatacgcagc cgcccgtctc gtgccgcaga acaccaggaa
     7621 caatgtcagc gatatgatct ccacaatgcc ggccggcgcg gccggagtca tctttccgat
     7681 ggccgcaaac gcccatagcg ccatgagacc ggcaagcccc gccgcgacgg tggcgccaat
     7741 tccctcgcgc catcccggac agaccggcat gaagagcgcc aatgccagcc ccagcgaaac
     7801 ggcgaggagc accttcgcgt tcaccaatgg cacaaaggaa aatgccggtt cggcgccgaa
     7861 ccaggccaag gccacgaaga tcagcgcgat gccggcgagg ctcatgcccc aacgcggcac
     7921 cgcggtcatg gcgatctttt cgaacaggcg ctggcggcgt tcgcgcacgc ggttcgccgt
     7981 ggcgacgaac atctcgtcga aatgcaggaa ccactcgccg atcggcatga tgatcaaggt
     8041 cgaggcggtt gccagaacga cgtccatcac cgcctggaaa gacgccgcac ctgccctggt
     8101 ccgccagaac catagtatcg acaacgccgt catcagcccc gccgccgcgg cagccggctt
     8161 gaacggatgc tctgcggtcc cgccgccgag cgtacggatc aggatattgt gcgccagcat
     8221 gacggcaatg gcgtaggcga agatataggc cagcaccggc acggcgccgt atgacgcatc
     8281 gccgctgacc ggcgccgccc aaatcaagcc cggcaaggga gccgccgccg tcagcaacgc
     8341 cagcgccaga tggcgcgtat gcagcaggga aacaaccgcc gccaaggccg cgcccgcaag
     8401 gatcaacccc gcattaccga gcgacgcctt ggcaaggatg gaattggccg tcgccacgcc
     8461 ggttgcgata ccgacgacgg aaagcgagag cgagagcagg aggacgcggt tgcggagcgc
     8521 gaattgcctt gccgcgccgg cctctccgcc gtttccagcc atatagattc accccgcgcg
     8581 gctggccggc ggctcgggca gatccatttc gagaatggcc atgttgaaag cgtaggaggt
     8641 ctcgccgtcc tcctcgtcct tgaagatcac gccgatgaat tcgccgttga cgtggacttc
     8701 gacggaatcc gcctgcttcg gccgctcgac cacggaaatc gtatcgagac gaaaaagatt
     8761 ccgtagatat ttctcgatgc gccagatttc gctgcgggtc acgggcccct ccttcacacg
     8821 gtgcgtcttg aactcgcatg acggccggaa ccggtcaagc gccaagatcg cacggccggg
     8881 catgtttcaa cggaaaggcg aacctaagca aaccgctaaa ctattgcacg gcggcgctag
     8941 tgtcccgatt ccgaaattcg caccacctct atatcggctg tgggcgaatt tcggaatcga
     9001 aaggacacta gcaacctgtt gaatcctagt gtcgttttgg tttcgaagtt cgcttgcagc
     9061 tcgatatagc agatagagcg aacttcgaaa ccacgacact agatactatc cgcctcgtcg
     9121 atgaactggt ccatctcctg cgaggggcgt tcgctgcccg cgcagttgat cagccttgcc
     9181 ggcacgccca ccgccgtgca atggggcgga atcgatttga gcaccaccga gcccgcgccg
     9241 acgcgcgaat attcgccgat ctcgatattg cccagtacct tcacccccat cgacaacaac
     9301 acgccgcggc ggaccttcgg atggcggtcg cccgtctcct tgccggtgcc gcccagcgtc
     9361 acgccgtgca gcatcgaaac gtcgtcttcc accaccgcgg tctcaccgat caccacgccg
     9421 gtggcatggt cgatcatgat gccgcggccg agcttggcgg ccgggtggat atcgacatcg
     9481 aacaattggg atatgcgact ctggaaaaac agggccatgc cgtcgcgctt ctgcgtccac
     9541 aaccaatggg cgatgcgata ggattcgagc gcttggaagc ctttgaagaa caagaacgct
     9601 tccgcatggg aatgacaggc gggatcgcgg tcgaacacgg ccgacagatc cgcccgcacg
     9661 gcctcgacga tggaaggatc ggcgtcgatc gcctcctcga aaatctcgcg ggtgagcatc
     9721 gggctcaaat cctcgccgcc tatcttcttg gcgaggtgat aggaaagtgc gtcctccagc
     9781 cggttatgct tgagaatggt ggcatgcacg aagctggtca gcgtcggctc gcgcagggcc
     9841 agactttggg cctgctcgcg caaggcgctc cagatcgggt cgcaactggc gactttttca
     9901 accgtaacgg ccattttgtc ctcccgcgaa ccaccttaca acaagccgga ctcgttgcca
     9961 aagcgaccgg tcgcccttcc cccccaccta gataggaagt ggcagccacg ctacaacatt
    10021 taaggatcac atgggttaaa catgaattcc cgccccaagg acatcttcaa caaggccgca
    10081 ccgcagacca tcgatctgtt gctctcccgg cgctccggca cggccaaagc catgaccggt
    10141 cccggccccg atgccgagga gctgcggacc atcctgcggg cggcggcccg cgtgcccgat
    10201 cacggcaggc tgttcccctg gcgcttcatc gtcttcgaag gcgacgcaag ggcgcgtttc
    10261 ggcaaactgc tcgtcgaatg cctcaaggaa accgacgccg tgatcgtgac gcccgagcgc
    10321 gctgcgcagg aggccggacg gtttctgcgc gccccggtcg tggtcggcgt ggtcagccgc
    10381 gtgcgcgcgg ccatccccat cccggaatgg gagcagcagc tttccgcggg cgccgtctgc
    10441 cagaccgtgc tgatcgcggc gcatgcactg ggcttcgtcg ccaactggct gacggaatgg
    10501 tacgcctatc atccactggt gtcggaacgg ctgggcctga agccgggcga gcggatcgcc
    10561 ggattcattt acatcggcaa gagcgcgatc gcgctcgaag accgtctgcg gccggacatg
    10621 gaaaagatcg tcacacgttt ctagggtctg gactcgatta ccgaattcct aatcaactac
    10681 ttgtcatggc ccgacgtctc ctggccatcc agcaggcgag gtccgtagcg acacagcgta
    10741 tggacgcgag ccaaagatac caacgatcac ctggatggcc cgcttttacg ggccatgaca
    10801 ctatgaatga aggttttctt attgagttca gaccctagac cgcccgcgcc tcaacccaga
    10861 aaccagcgcc acagcgccag cagctcgccc gcgacgaacc aggt
//
LOCUS       DASWPU010000057         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_55544,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000057 DASWPU010000000
VERSION     DASWPU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55544"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..413)
                     /gene="rplL"
                     /locus_tag="VGC27_01230"
                     /pseudo
     CDS             complement(42..413)
                     /gene="rplL"
                     /locus_tag="VGC27_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
     gene            complement(464..979)
                     /gene="rplJ"
                     /locus_tag="VGC27_01235"
     CDS             complement(464..979)
                     /gene="rplJ"
                     /locus_tag="VGC27_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY0281226.1"
                     /translation="MEKAKKSEVVEELKGVFANAGSVVVAHYTGMTVAQMGDLRSRMR
                     VAGASFKVAKNRLAVRALQGTAVEAISDLFKGPTGIAYSKDPIAASKVAVAYAKDNAK
                     LVILGGSVGVTMLDAEGVKALASLPSLDELRGKIAGLLKAPATKIAGIVQAPAGQLAR
                     VVGAYSKKEAA"
     gene            complement(1335..2045)
                     /gene="rplA"
                     /locus_tag="VGC27_01240"
     CDS             complement(1335..2045)
                     /gene="rplA"
                     /locus_tag="VGC27_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY0281227.1"
                     /translation="MTKISKRFKKVVEGVDANKAYSVEEAVKLIKARATAKFDETIEL
                     AINLGVDPRHADQMVRGVVSLPNGTGRTQRVAVFAKGPKADEARAAGADIVGAEDLAE
                     TVQKGEINFDRCIATPDMMGVVGRLGKVLGPRGLMPNPKVGTVTMDVTQAIKDAKGGA
                     VEFRVEKAGIVQAGVGKASFSEDAIVQNVKTFVDSVIKAKPQGAKGTYVKKVSISSTM
                     GPGVKIALPSIGAASAEA"
     gene            complement(2051..2479)
                     /gene="rplK"
                     /locus_tag="VGC27_01245"
     CDS             complement(2051..2479)
                     /gene="rplK"
                     /locus_tag="VGC27_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY0281228.1"
                     /translation="MAKKIIGYIKLQVPAGAANPSPPIGPALGQRGLNIMEFCKSFNA
                     KTQDLEKNMPIPVNITVFSDKSFTFEMKTPPASFFLKKAAKLGSGSKTPSRDIAGSVT
                     KAQVRQIAEAKLKDLNANDVEMAMRMIEGSARSMGIEVKE"
     gene            complement(2593..>3059)
                     /gene="nusG"
                     /locus_tag="VGC27_01250"
     CDS             complement(2593..>3059)
                     /gene="nusG"
                     /locus_tag="VGC27_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY0281229.1"
                     /translation="RRQAQLQGQEDLIEEVLVPTEEVIEVRRGQKVNAERKFLPGYVL
                     VRAKLTDEAYHLIKNVAKVTGFLGQNNKPMPLRQSEVDRILNQVTEGAERPKPSITFE
                     IGEQVRVADGPFTSFNGTVEEVDEDRSRLKVAVSIFGRATPVELEFTQVEKV"
BASE COUNT          507 a         1044 c          827 g          681 t
ORIGIN      
        1 aatatgcacg cgcacgcggt ttttcaagcc gcagcgcctc cttacttcaa ctcgactttc
       61 gcgcctgcgt cttcgagctg tttcttgatc ttggccgctt cgtccttgtt cacatcggcc
      121 ttgacctctt tgggagcgcc ctcgacgagg tctttcgctt ccttcaggcc caaaccggtg
      181 atcgcacgga cttccttgat gacgttgatc tttttgtcgc cggcagccac aagcacgacc
      241 gtgaattcgg tcttctcgac cgcagcagcc gccgccgcga ccggcgcagc cgcagccgcg
      301 accggagccg ccgccgacac gcctcatttg tcctcaagca gcttcgcaag ctcggccgct
      361 tccagaaccg tgagggccga caaatcttcg accagtttct cgatattcga catttcttat
      421 ttccttcaag tgcttacggg tttgaatgtt gtttggatgc cgcctaggcc gcttctttct
      481 tggaatacgc gccgacaacg cgggcaagct gtccggcggg cgcctgaaca attccggcga
      541 tcttggtcgc cggagccttc agcaggcccg caatcttgcc tctcagctca tcgagcgacg
      601 gcaggctggc gagggccttc acaccctcgg catcgagcat cgtcactccg accgaaccgc
      661 ccaggatcac gagtttcgcg ttgtccttgg cgtaagccac cgccaccttc gaagccgcga
      721 tcgggtcctt gctgtacgca atgcccgtcg gacctttgaa gagatcggag atggcctcca
      781 cggccgttcc ctgaagcgca cgaacggcaa gacggttctt cgccaccttg aacgacgctc
      841 cggccacgcg catgcgcgac ctgagatcgc ccatctgggc aaccgtcata ccggtatagt
      901 gggcgacgac cacggagccg gcattcgcga agacgccttt cagctcttcg acgacttccg
      961 actttttggc tttctccact gtgctctcct tttccctggc ctaccgcttc gctgcttgag
     1021 gccaagtctc aaccggcgat gcgtgacgac ccgcatcccc gccctgttac cagaccatcc
     1081 ttcgcgcgtt tcgcgcttcg gacggccact cctgccccag ggttcaaacc cgaacggcgg
     1141 aatctcttcc gcaaattcgg ttctcgcccc gtctgcgctg gaacgtttaa gtccttaagg
     1201 actccagcgg tctcggacag gtcccggcga tcgtccgccg gaatttgacc tccctcatcc
     1261 tgagcttgtc gaaggatgag ggccgaaact gtgtccttca tggttcgaca agctcaccat
     1321 gaagggactt tcctttaagc ctccgcgctc gccgcgccga tggacgggag cgcgatcttc
     1381 acgcccggac ccatcgtcga actgatggaa accttcttca cataagtgcc cttggcgccc
     1441 tgcggcttgg ctttgatgac gctgtccacg aacgtcttca cattctgcac gatcgcgtct
     1501 tcggaaaagc tcgccttgcc gacgcccgcc tgcacgatgc ccgccttctc gacgcggaat
     1561 tccacggcgc cgcccttggc gtccttgatc gcctgggtca cgtccatggt caccgtgccg
     1621 accttcggat tcggcatcag gccgcgcggg cccaacacct taccaagacg gccgacgacg
     1681 cccatcatgt caggcgtcgc gatgcagcgg tcgaaattga tctcgccctt ctgcaccgtc
     1741 tccgccagat cttcggcgcc gacgatgtcc gcgccagcgg cgcgcgcttc gtccgccttc
     1801 ggtcccttgg cgaacaccgc gacgcgctgc gtacggccgg tgccgttggg cagcgagaca
     1861 acgccgcgta ccatctgatc ggcatggcgc ggatcgacgc ccaaattgat cgccaattcg
     1921 atcgtttcgt cgaacttcgc ggtggcccgc gccttgatca gcttcaccgc ctcctcgacg
     1981 gaataggcct tgttcgcgtc cacgccttcg acgacttttt tgaatctctt cgatatcttg
     2041 gtcatggcgc ctactccttg acctcgatgc ccatcgaacg cgccgaacct tcgatcatgc
     2101 gcatggccat ctcgacgtcg ttggcgttca aatccttcag cttggcttcg gcgatctggc
     2161 ggacttgcgc cttggtcacc gagccggcga tatcgcggct cggcgtcttc gatcccgatc
     2221 ccagcttcgc ggccttcttg aggaagaagg acgcgggcgg cgtcttcatc tcgaaggtga
     2281 aagacttgtc cgaaaacacg gtgatgttca ccggaatcgg catgttcttt tccaggtcct
     2341 gcgtcttggc gttgaacgac ttgcagaact ccatgatgtt gaggccgcgc tggcccaaag
     2401 caggcccgat cggcggagag gggtttgccg cccccgccgg tacctgcagc ttgatgtaac
     2461 cgataatctt cttcgccact ttcggtccgc ctccttagcg gtttgcggtg caaacggagc
     2521 tggccgcccc tcccgcgtcg tgcaccactc gagctaggcc ctgcgagccg ccctcggatg
     2581 cgattccaat aactacactt tctcgacttg cgtgaactcc aactccaccg gcgttgcgcg
     2641 gccgaagatc gaaaccgcca ctttcaagcg cgaacggtct tcgtccactt cttccaccgt
     2701 accgttgaac gaggtgaacg ggccgtccgc cacgcgtacc tgctcgccga tttcgaacgt
     2761 gatcgacggc ttcggccgct cggcgccttc ggtcacctga ttgagaatgc ggtcgacctc
     2821 gctctggcgc agcggcatcg gcttgttgtt ttgaccgagg aagcccgtca ccttcgccac
     2881 gttcttgatg agatggtagg cctcatccgt cagcttggcg cgcaccagca cgtagcccgg
     2941 caaaaacttg cgctcggcgt tgaccttctg gccccggcgc acttcgatca cttcctcggt
     3001 gggaacaagg acttcctcga tgaggtcttc ctggccctga agttgagcct ggcgcctga
//
LOCUS       DASWPU010000058         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_67814,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000058 DASWPU010000000
VERSION     DASWPU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67814"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /gene="guaB"
                     /locus_tag="VGC27_01255"
     CDS             <1..1318
                     /gene="guaB"
                     /locus_tag="VGC27_01255"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY0281230.1"
                     /translation="LAIAMAQSGGIGVIHRNLSIEEQAEHVRRVKKYESGMVVNPLTI
                     GPDATLADALALMEHHHISGIPVVVGASPAGAGKLVGILTNRDVRFATDTSLKVAELM
                     TKENLVTVREGVKMDEAKKLLHARRIEKLLVVDDAYRCVGLITVKDIEKAQKFPNACK
                     DERGRLRVAAAVNVGADGLKRAMALVAAECDVVVVDTAHGHSKGVLDAIAAIKRESNY
                     TQVLAGNIATADGAKALIDAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIMDAVSAA
                     RKQGIPVVADGGIRYSGDLAKAMAAGADVVMVGSLLAGTEESPGEVFLYQGRSYKGYR
                     GMGSLGAMARGSADRYFQAEVKDSLKLVPEGVEGQVPYKGPVSGVIHQVVGGLRAAMG
                     YTGCKTIEDFHKNSQFVRITGAGMAESHVHGVVVTRESPNYPGVVQ"
     gene            1319..>1644
                     /locus_tag="VGC27_01260"
     CDS             1319..>1644
                     /locus_tag="VGC27_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281231.1"
                     /translation="MTPSARLQAVIDILEGLEATAQPADRFLRDWARARRYAGSKDRA
                     AIAERTYAVLRHRESFAWRMGSADARALVIASLLAEGLSADAIADLFDGKAYGAPALS
                     EAERRAL"
BASE COUNT          282 a          532 c          575 g          255 t
ORIGIN      
        1 gctcgccatc gccatggcgc agtcgggcgg catcggggtg atccatcgca acctcagcat
       61 cgaggaacag gccgagcatg tccgccgggt gaagaaatac gaaagcggca tggtggtgaa
      121 cccgctgacc atcgggcccg acgccaccct ggccgacgcg ctggccttga tggagcatca
      181 ccacatctcc ggcattccgg tggtggtcgg cgcttcgccc gccggcgcgg gcaagctggt
      241 cggcatcctc accaaccgcg acgtgcgctt cgccaccgac accagcctca aggtcgccga
      301 gctgatgacc aaggagaacc tcgtcaccgt gcgcgaaggc gtcaagatgg acgaggccaa
      361 gaagctgctg cacgcgcggc gcatcgaaaa gctgctggtg gtggacgatg cctatcgctg
      421 cgtcgggctg atcaccgtca aggacatcga gaaggcgcag aaattcccca acgcctgcaa
      481 ggacgagcgc ggccgcctgc gggtcgccgc cgccgtcaat gtcggggccg acggcctcaa
      541 gcgcgccatg gcgctggtcg cggccgaatg cgacgtggtg gtggtcgata ccgcccacgg
      601 ccattccaag ggcgtgctcg acgccatcgc cgccatcaag cgcgagagca attacaccca
      661 ggtgctcgcg ggcaacatcg ccaccgccga cggcgccaag gcgctgatcg atgcgggggc
      721 cgacgcggtg aaggtgggca tcgggccggg atcgatctgc accacgcgca tcgtcgcggg
      781 cgtcggcgtg ccgcaactga ccgcgatcat ggatgcggta tcggcggcgc ggaagcaggg
      841 cattccggtc gtggccgacg gcggcatccg ctattccggc gacctggcca aagcgatggc
      901 ggcgggcgcc gacgtggtga tggtgggatc gctcttagcg ggcacggaag aaagccccgg
      961 cgaggtgttc ctctatcagg gccgttccta taagggctat cgcggcatgg gttcgctggg
     1021 cgcgatggcg cgcggttcgg ccgaccgtta cttccaggcc gaggtgaagg attcgctcaa
     1081 gctggtgccg gaaggcgtcg aaggccaggt gccgtacaag gggccggtca gcggggtgat
     1141 ccatcaggtc gtcggcggct tgcgcgcggc gatgggctat accggctgca agaccatcga
     1201 agacttccac aagaattcgc aattcgtgcg catcaccggc gcgggcatgg ccgaaagcca
     1261 cgtccatggc gttgtggtca cgcgcgaatc ccccaactat ccgggcgtgg tgcaataatt
     1321 gacgccgtcc gcgcgcctgc aagcggtcat cgatatcctc gaagggcttg aggcgacggc
     1381 gcagcccgcc gaccgttttt tgcgcgattg ggcccgcgcc cgccgctatg ccggctccaa
     1441 ggaccgcgcc gcgatcgccg aacgcactta cgcggtgctg cgccatcgcg agtcctttgc
     1501 ctggcggatg ggcagcgccg atgcgcgggc gctggtgatc gcgtccttgc tggccgaagg
     1561 tctttcggcg gacgccattg cggatctgtt cgacggcaaa gcctatggcg cgcccgcctt
     1621 gagcgaagcc gagcgccgcg cgct
//
LOCUS       DASWPU010000059         3505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_18810,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000059 DASWPU010000000
VERSION     DASWPU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18810"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VGC27_01265"
     CDS             complement(<1..432)
                     /locus_tag="VGC27_01265"
                     /inference="COORDINATES: protein motif:HMM:NF020068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY0281232.1"
                     /translation="MFAAALFAMNQNALAQVPVGAGGQIQQIPQAPVLQKTVPDLPTR
                     RDETVPPPEAAGPKFLVTSLHVTGQTRFTEAELIAATGFKPGSELSLADLRVLASKIT
                     HLYNRNGYFVAQAYLPPQDIKDGAVTIAVIEGRYGKIGLDNQ"
     gene            complement(976..1683)
                     /locus_tag="VGC27_01270"
     CDS             complement(976..1683)
                     /locus_tag="VGC27_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281233.1"
                     /translation="MAAITAAGLCLGVFAAIPTFSQAAVEHHSHLFVASGRPIETQPS
                     ISPRPILANFLGEPASDDARQIADWVVYSGDNRGLPFVIVDKKDTKVFAFDGHGYLLG
                     ATPALLGLARGDDSAPGIGDRELSDIRPEERTTAAGRFVSALGRNLRGQEVLWVDYDA
                     ALTLHRVLARNTKEARLQRLAAASPLPRRITFGCINVPASFFDGVVHPAFTGTNGVVY
                     ILPEIKSIREVFFTSIS"
     gene            2357..>3505
                     /locus_tag="VGC27_01275"
     CDS             2357..>3505
                     /locus_tag="VGC27_01275"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY0281234.1"
                     /translation="MWKRTRFCRLTFLGVCAVLAGTADKSAQAADPQPYKIVFPATGS
                     SPIDEALRASAQLVTLNSKAPVPPFALIERAKDDIPRLQTALDSFGYYQNNVTITIAD
                     RDLADADLPVVLDAVPQGTAVTVKVSVTMGPLYRLGNITIEGLLLESARAALGIKSGD
                     PALAGAVLDAQTRLVSALQEDGYALAKVDSPVATANDAAHLIDVVYKVAAGPQIDIGA
                     IDFKGLKDVNESFARRALTVKSGERYRPSRIEAARQSLAGLGVFSGVSVHAADSLSAD
                     GRIPLTFDVQERKPHAVTLAGTYSTDLGISFSTTWSHRNLFGNAEQLNLTAAAQLGGN
                     ALTKPGYQVGAQFIKPDFLARDQALELDLNAVKQSLQAYDQTALLEKIA"
BASE COUNT          740 a         1064 c         1037 g          664 t
ORIGIN      
        1 ctggttgtcc aggccgatct ttccgtagcg gccttcaatc accgcaatcg ttacggcccc
       61 atccttgatg tcctggggag gcagataggc ttgcgccacg aaatatccgt tccgattgta
      121 gaggtgcgtg attttcgaag ccagcacgcg caaatcggcc aaactcaatt cactgcccgg
      181 cttgaatccg gtggccgcga tcaattcggc ttcggtaaac cgcgtctgcc cggtcacgtg
      241 cagcgacgtg accaggaact tgggtccggc agcctcggga gggggtacag tctcgtctcg
      301 cctggttgga aggtccggaa ctgttttttg cagaacaggg gcctgcggaa tttgctgaat
      361 ttgcccgcct gcgcccaccg gcacttgggc gagagcgttt tggttcatcg caaacagagc
      421 ggccgcaaac aagctcgatt tcaggacaag gccggcgaac ccggcccgga cgaatttgtg
      481 gccgccccgc tcaaaagcgg cgtgcagaag tgcgacaagg tgatttcgtg tggtgcacat
      541 cgcagtcccc cctactagaa agagcctagc cgtcatccag ggcgattaac cgtccgccac
      601 agaaaaggct tccgcgcaaa cggtgccgga atcacataga atcgcctccg gcttacttta
      661 gtgggcgcga gaacgacctg ggaggttcgg aatctaccta ggtaccctac tggtacctag
      721 tgtcccgatt ccgaaattcg cccacagccg gtatagagag ggtgcgaatt tcggaatcga
      781 aaggacacta gcaacctatt gaatcctagt gtcgttttgg tttcgaagtt cgcttgcagc
      841 ccgacatggc gcatgctgcg aacttcgaaa ccacgacact aggtacaggt tgtactgcct
      901 ggggcgattc gaatgccatt cacgaggcag gcgagcctgt acgggaaggc gtgggcgcga
      961 acgcgccggt cccactcatg agatagaggt gaagaaaact tcgcggatcg atttgatttc
     1021 cggcagaata tagacgacgc cattcgtccc cgtaaacgcg ggatgcacaa cgccgtcgaa
     1081 gaaacttgcc ggaacattga tgcagccaaa agtgattcgg cggggaaggg gtgatgccgc
     1141 cgcgagtcgt tgcaggcgcg cctccttggt atttcttgcc aacacacgat gcagggtcaa
     1201 agcggcatcg taatccaccc agagaacctc ctgcccacgc aaattacggc ccaaagcaga
     1261 cacaaagcgt cccgccgccg tcgtgcgctc ctccggacgt atgtccgaca gttcccggtc
     1321 cccgattcca ggagccgagt cgtccccccg agccaatccg agcaacgcgg gagtggcgcc
     1381 gaggagatat ccatgcccgt cgaatgcaaa gacttttgtg tctttcttat cgacaatcac
     1441 gaacggcaag ccgcgattgt cgcccgaata gaccacccag tccgcgattt ggcgcgcgtc
     1501 gtctgacgca ggctctccca ggaaattagc cagtatcggc cgaggcgata ttgaaggctg
     1561 tgtctcaatg ggacggccgc ttgcgacgaa caagtgtgaa tgatgttcca ccgccgcttg
     1621 agagaatgtg ggaatggccg caaacacgcc gaggcaaagt cctgcggccg tgatcgccgc
     1681 catcttgcgc attcggcgcg gggatttcgc aatccttaac gcacccaaag tgcgcgatcc
     1741 gatatttacc ctcttcgatg acgcgaggtc agacatagtc cacccacggg ttgtcgctcg
     1801 agcgggatga agcttggtcc ttggtgttga gaccaacttg caggcccgat ggaaaacggg
     1861 cccggtacgg tcgctcgcat aggcacggcg ccagctaacc cgttctgata gccacaccgc
     1921 cgccaagact tgagcaacgc ccgtgccagc aaatttgggg gttttggggt tatttgtgtc
     1981 tcattgcgaa acgtcggccg ccgtgaagac acagccgaag accgatcggc ccctggcaca
     2041 gccgttcgtt agaggcaagg gtcacgcttg agattcggcc gcgtttctcg cgcaagaccg
     2101 aaatggaaag cggacatgcc atgcccgtaa aaatagctcg gatgaggaac cggcaggcgt
     2161 gggaaggctt gggcgacatc aaaccttccg tggcgggcct gttcgagccg gcatggtgat
     2221 catcgcaggc ggtggcttcg gtcatggtga cggcatagag tgaaaccgtg gccctcggtc
     2281 ccggtaacga atggaaccga tcatccccaa agcagtcttc caaccccgcg cgtcgcaagg
     2341 tacgctttcg tcaagtatgt ggaagcgcac gcgattctgc cggctgacgt ttctgggtgt
     2401 ttgcgccgta ctggcgggga ccgccgataa atctgcacaa gccgccgatc cgcagcccta
     2461 caaaattgtg tttcccgcta cggggtcttc cccgatcgac gaggcgttgc gggccagcgc
     2521 ccagctcgta acgctgaaca gcaaggcgcc ggttccgccc ttcgccctga tcgagcgcgc
     2581 caaggacgac attccgcgcc ttcagacggc attggacagc tttggctact accagaacaa
     2641 tgtgaccatc accatcgcgg accgcgacct cgccgatgcc gatcttcctg tcgttctgga
     2701 tgcggttccc cagggaaccg cggtgaccgt gaaagtttcc gtgacgatgg gaccactcta
     2761 tcgtctggga aacatcacca tcgaaggttt actgctcgaa agcgcccgcg cggcgttagg
     2821 catcaagagc ggcgatccgg cgctggcggg cgccgttctg gacgcgcaaa cgcgcctcgt
     2881 cagcgcattg caggaggacg gttatgcgct ggcgaaggtg gatagccccg tcgccacggc
     2941 caacgacgcc gcgcacctga tcgatgtcgt ttataaggtt gcggccgggc cgcagatcga
     3001 catcggagcg attgacttca aaggcctgaa ggacgtcaac gagtctttcg cgcggcgcgc
     3061 cctgaccgtc aaatccggcg aacgctatcg tcccagcagg atcgaagcgg cgcgccagag
     3121 ccttgccggc ctgggcgtgt tttccggcgt cagcgtccat gctgcggaca gtctctcggc
     3181 cgacggccgc attcctctca ccttcgacgt ccaggaacgc aaaccgcacg ccgtaacgct
     3241 ggcggggaca tattccaccg atctcggcat cagtttttcg acgacatggt cgcaccgcaa
     3301 cctgttcggc aacgccgaac aactgaacct gacggccgcg gcccagcttg gcggcaacgc
     3361 cctgaccaaa cccggctacc aggtcggggc gcagttcatc aaaccggatt tcctggcccg
     3421 cgaccaggcg ctcgaactcg acctcaatgc cgtcaagcag agcctgcagg cgtacgacca
     3481 gaccgcattg ctggaaaaga tcgcc
//
LOCUS       DASWPU010000060         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_79905,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000060 DASWPU010000000
VERSION     DASWPU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79905"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1043)
                     /locus_tag="VGC27_01280"
     CDS             complement(72..1043)
                     /locus_tag="VGC27_01280"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY0281235.1"
                     /translation="MIRSHVIGCGAYLPKTILTNHDLAKREDTSDEWIVARTGIRERH
                     VAGPGEKTSDLAFEAAQAALADAGASASDIDLIVCATTTPDETFPAVAAMLQGRLGMT
                     RGAAFDVQAVCSGFIFGLAVADNFIRCGQAKTALLVGADCMTKLLDWNDRTTSVLFGD
                     GAGAVLLKAGEGTGSNEDRGVLNTKLYSDGRLHDLLYVDGGPSTTQTTGHLRMQGREV
                     FRHAVTNIAASIVASLEEAGLGIEDIDWFVPHQANLRILDGTAKKLGIPPEKVVVTIA
                     KHGNTSAASVPLALAAAVGDGRIKRGDFVLLEAMGGGLTWGASLVRW"
     gene            complement(1040..2092)
                     /gene="plsX"
                     /locus_tag="VGC27_01285"
     CDS             complement(1040..2092)
                     /gene="plsX"
                     /locus_tag="VGC27_01285"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY0281236.1"
                     /translation="MTNVSRELTVSIDAMGSDAGPSIVVSALARSLLRHPPVKFILHG
                     DEAIIDKLLGKRRAKFAGRVSVRHAETYVRMDEKPSLALRRGRKTSMWRAIESVKNGE
                     AEAVVSAGNTGALMAMAMYQLGVIDGISRPAIAALWPTRRGQCVVLDMGANIECDADQ
                     LVDFAVMGEAFARAVLGIKRPSVGLLNVGSEEVKGHDAVKGAAQMLRDVSLPMEFAGF
                     IEGDDIAEGTVDVAVTDGFTGNIALKTAEGTAKLVVGYLRAALKRSFASRLGAFLASG
                     ALNALRRKLDPRTSNGGMFLGLNGVVVKSHGGTDALGFASATDMAIDMAEGGINKSIV
                     ADRAGVSTISREAVTT"
     gene            complement(2133..2312)
                     /gene="rpmF"
                     /locus_tag="VGC27_01290"
     CDS             complement(2133..2312)
                     /gene="rpmF"
                     /locus_tag="VGC27_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY0281237.1"
                     /translation="MAVQKSKKSTSRRNMRRAHHALAPTTHNECPNCGEQKRPHHVCA
                     ACGYYDGREVAKAKA"
     gene            complement(2455..2985)
                     /locus_tag="VGC27_01295"
     CDS             complement(2455..2985)
                     /locus_tag="VGC27_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281238.1"
                     /translation="MNDEQTAPIHRLYELGDLSQAGSLIKIAADGEDLKRLAHWANVD
                     SVRAFSARIELRRLSRTSFSFEAELEADIVQSCVVTLDPVATHIAKHIARELHFSPLP
                     REEGGELTLSAGDEDVPEEISSLDYDLAAPLLEEFLLAIDPYPRKEGVAFEPPAQPAE
                     PPQSPFAVLKRLNEPG"
BASE COUNT          545 a         1013 c          987 g          585 t
ORIGIN      
        1 acccggtaag cctattcggg atggggattt gccgtcaaca tcaaagggat aggtggagcc
       61 gctcagacct gttaccaacg caccaggctg gcgccccagg tcaggccgcc gcccatggcc
      121 tccagaagaa caaaatcccc ccgttttatc cggccatcgc ccacggccgc ggccaaggcc
      181 aggggcacgg aagccgccga ggtgttgccg tgctttgcga tggtgacgac caccttttcg
      241 ggtggaatcc ccagcttttt tgcggtcccg tcgaggattc gcagattggc ctggtgcggt
      301 acgaaccagt cgatatcctc gatgcccagc cccgcctctt ccagagaggc cacaatcgag
      361 gcggcgatat tggtgaccgc gtggcgaaac acttccctgc cttgcatgcg caggtgaccg
      421 gtcgtctggg tggtcgacgg gccgccatcc acataaagca ggtcgtgcag cctgccgtcc
      481 gaatagagct tggtgttgag gacgccgcga tcctcgttgc tcccggtccc ctccccggct
      541 ttcagcagca ccgcgcccgc cccgtcgccg aacaacacgc tggtcgtgcg gtcgttccag
      601 tcgagcaact ttgtcatgca gtccgcgccg accagcagcg ccgtcttggc ctggccgcag
      661 cggatgaaat tgtcggcgac cgccagcccg aagatgaagc cggagcaaac cgcctggacg
      721 tcgaacgcgg cgccgcgcgt catgcccagc cgtccttgga gcatggcggc aacggccgga
      781 aacgtctcgt cgggcgtcgt cgtggcgcag acgatgagat cgatatcgct tgccgacgcg
      841 ccggcatcgg ccaaagccgc ctgcgcggcc tcgaacgcca gatcggaagt cttttcaccc
      901 ggcccggcga cgtggcgctc gcggattccc gtgcgcgcga cgatccattc gtcgctggtg
      961 tcctcccgct tggccagatc gtggttggtg aggatggtct taggcagata ggcgccgcaa
     1021 ccgatgacat gggaacggat cacgtggtga cggcctcccg gctgatcgta ctcacgccgg
     1081 ccctgtccgc gacgatgctc ttgttgattc cgccttcggc catgtcgatg gccatgtcgg
     1141 tggcgctggc gaatccgagc gcgtcggttc cgccgtggct cttcaccacc acgccgttga
     1201 ggccgaggaa catcccgccg ttcgaggtgc gcggatcgag cttgcggcgc agcgcgttca
     1261 gcgccccgct cgccaggaaa gcacctaacc gcgaggcaaa ggagcgcttg agagcggcgc
     1321 gcagatagcc cacgaccagc ttggcggtgc cttccgccgt cttgagcgcg atattgccgg
     1381 tgaagccgtc ggtcaccgcc acgtcgacgg tgccttcggc gatgtcgtcg ccttcgatga
     1441 acccggcgaa ctccatcggc agcgagacgt cgcgcagcat ctgggcggcg cccttcaccg
     1501 catcgtggcc cttcacctct tccgaaccga cgttgagcag gcccacggac ggacgtttga
     1561 tcccgagcac ggcgcgcgcg aaagcctcgc ccatcaccgc gaaatcgacg agctgatcgg
     1621 cgtcgcattc gatgttggcg cccatgtcga gaaccacgca ctgtccccgc ctggtcggcc
     1681 acagagccgc gatcgccggc cgcgaaatcc cgtcgatcac gccgagctga tacatcgcca
     1741 tcgccatcag cgctccggtg ttgccggcgg agacgacggc ttcggcctca ccgttcttca
     1801 ccgattcgat ggcgcgccac atgctggttt tgcgtccgcg gcgaagcgcc agactcggct
     1861 tttcgtccat gcgtacatag gtctcggcgt ggcgcacgct gacgcggccc gcgaacttgg
     1921 cgcggcgctt gccgagcagc ttgtcgatga tcgcttcgtc gccatgcaga atgaacttga
     1981 cgggggggtg gcgcaggagc gagcgggcga gcgcggacac gactatggat gggccggcgt
     2041 cgctgcccat cgcatcaatc gaaaccgtca attcgcggga cacgttcgtc actgcccctc
     2101 agagtggccg ccggatgctc tcgccgtgcc ggtcaggcct tggccttggc cacttcgcgg
     2161 ccgtcgtaat agccgcaagc ggcgcacaca tgatgcggac gcttctgctc gccgcaattg
     2221 gggcattcgt tatgggtcgt gggcgccaga gcgtggtgcg cgcggcgcat gttgcgccgc
     2281 gatgtcgact ttttactttt ttggaccgcc atggcggaaa tcttctccat cgggcacggc
     2341 cggaacggcg cagaaacagt gcgcgaccgt tcggaccggc aggcaaagca aggcgcggag
     2401 tgaacaggaa accgtccccg gattcaagcg cgaaaaacgg ccgttttccg cgattcagcc
     2461 gggctcgttg agcctcttca gcaccgcgaa cgggctttgg gggggctctg cgggttgtgc
     2521 gggcggctcg aacgccaccc cttccttgcg ggggtaggga tctatcgcca gaaggaattc
     2581 ctccagcaac ggcgccgcca ggtcgtaatc gaggcttgag atctcctccg ggacgtcttc
     2641 atcgcccgcc gaaagggtca attcgccgcc ctcttcgcgc ggaaggggcg aaaaatgcag
     2701 ttcgcgcgcg atgtgcttgg cgatatgcgt ggccaccgga tcgagcgtca ccacgcagct
     2761 ttgtacgatg tcggcctcca gctccgcttc gaacgagaaa cttgtccgcg acaggcggcg
     2821 cagttcgatc cgggcgctaa aggcccgcac agaatccaca ttggcccaat gggccaagcg
     2881 tttgagatcc tcgccgtcgg cggcgatttt tatcaggctg ccggcctgcg acagatcgcc
     2941 aagttcataa agacgatgga tcggtgccgt ttgttcgtcg ttcatcgggc tagtgtcgtg
     3001 gtttcgaagt tcgcaccatt tgttatatct ggctgcaagc gaacttcgaa accaaaacga
     3061 cactaggatt caacaggttg ctagtgtcct ttcgattccg aaattcgccc ggagccgata
     3121 taaagatagt
//
LOCUS       DASWPU010000061         1961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_37254,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000061 DASWPU010000000
VERSION     DASWPU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37254"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VGC27_01300"
     CDS             complement(<1..1144)
                     /locus_tag="VGC27_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281239.1"
                     /translation="MARMRGPVGALCLGVGAIVALAGCTETVNEGVTDYRKATINSQS
                     AQGAPTDISKKYALSNTTDFSGNGAIPITAGRRYYVSLLQAFIGPEARVGGTNWFREH
                     EQVALVLRVHDSNDRTRPGRLVFFTDDLKLGGQFLNFSNLLTIGPTDYKGGVITIDVD
                     EVHLRGTTNHIKERLQELAQEPYCPLGAHPASSGHDLCDDEPAPDAGGASDSAQSALV
                     GPAPNKRRTWQARERDLFNLIDTDGYSTRYTLTLLPAGGVVGLPYPRFEAGNYVLMRH
                     DKRDNAFEWGRLQLDNNTGRLVYAATRSDAGGQLASRDPHDDKDLNYQSRKDRFASSG
                     GKALWQEQTGDYREQSYVTLQINALDDPAPPTVINPPRTRSLTQEQT"
     gene            1382..1942
                     /locus_tag="VGC27_01305"
     CDS             1382..1942
                     /locus_tag="VGC27_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEY0281240.1"
                     /translation="MLRLLALVLLTAALAAPAAAAGARAAAKLIGLDGKPMGRASFRQ
                     TAHGVLIEIDLRGLKPGAHAIMIHTTASCDPKKLFTSAGPDLSFEPTKPHGFLAKGGP
                     RAGDLPNQFAHADGTLHAAMITNAFSLGNGKKSIFDRDGASIIVHERADDYTTQPDGN
                     AGPRLACGTIIRTQGPRKGVPHRAHK"
BASE COUNT          337 a          618 c          635 g          371 t
ORIGIN      
        1 tcgtctgttc ctgggtcagc gagcgcgtac gcggcggatt gatcaccgtg ggcggcgccg
       61 ggtcgtcgag cgcgttgatc tgcagcgtca cataggactg ttcgcggtaa tcgccggtct
      121 gctcctgcca gagagccttg ccgccgctcg aagcgaagcg gtccttgcgg ctctgatagt
      181 tcaagtcctt gtcgtcgtgc ggatcgcgcg aggcaagctg gccgccggca tcgctgcgcg
      241 tcgcggcata gacgaggcgg ccggtattgt tgtcgagctg caaccgtccc cactcgaagg
      301 cgttgtcgcg cttgtcgtga cgcatcaaca cgtaattgcc cgcctcgaag cgcggataag
      361 gcagtcctac cacgccgccc gcgggcagca gcgtcagggt gtagcgcgtg gaatagccgt
      421 cggtgtcgat cagattgaac agatcgcgct cgcgcgcctg ccaggtgcgg cgcttgttgg
      481 gcgccgggcc gaccagggcc gactgcgccg aatccgatgc accgccggca tcgggcgccg
      541 gttcgtcgtc gcacaggtcg tgaccgctgg aggcgggatg ggcgccgagc gggcaatagg
      601 gttcctgcgc cagctcttgc agcctttcct tgatatggtt ggtggtgccg cgcagatgca
      661 cttcgtccac gtctatggtg atgacgccgc ctttgtaatc ggtagggccg atggtgagca
      721 ggttggagaa gttgaggaac tgtccgccca gcttcagatc gtcggtgaag aacaccagcc
      781 ggccgggacg ggtgcggtcg ttgctgtcgt gaacgcgcag caccaaggcc acctgttcat
      841 gttcgcggaa ccagttggtg ccgcccaccc gcgcctcggg accgatgaag gcttgcagca
      901 gagagacgta atagcggcgg cccgcggtga tcgggatggc gccgttgccg gagaaatcgg
      961 tggtgttgga gagagcatac ttcttggaga tatcggtcgg tgcgccttgc gccgactggc
     1021 tgttgatcgt cgccttgcgg taatccgtga cgccttcgtt caccgtctcc gtgcagcctg
     1081 ccagcgccac gatcgcgccg acaccgagac acagcgcgcc gaccggcccg cgcatccgag
     1141 ccatagctcc agcagacatc accgccccca acattccaca acttcgtttg gacgtgattc
     1201 cggcttcaaa cgtgcaaaga gtcaacctta agagttaagg ttgattcggt gaagtttcag
     1261 cgcttcgttt tcggcatgag aaattattca ttcctttcag cgcgttacaa cacccgctca
     1321 gccgccttcc ggcgctggcg cggttttcgg tgcattaaat tcggggggtc agatgaaata
     1381 catgctgcgg ttgcttgctc tggttctgtt gacggcggcg ctcgccgcac ccgccgccgc
     1441 ggccggcgcg cgggcggccg cgaaattgat cgggctggac ggcaagccga tgggccgggc
     1501 gagctttcga caaaccgcgc acggcgttct gatcgagatc gacctgcgcg ggcttaagcc
     1561 cggggcgcac gcgatcatga tccacaccac ggcgtcgtgc gatccgaaga agctcttcac
     1621 ctcggcgggg cctgatctct ctttcgaacc gaccaagccg cacggtttcc tcgccaaggg
     1681 cggtccgcgt gccggcgacc tgcccaatca attcgcgcac gccgacggaa ccctgcacgc
     1741 cgcgatgatc accaacgcct tctcgctggg caacggcaag aaatcgattt tcgaccgcga
     1801 cggcgcctcg atcatcgtgc atgaacgggc cgacgattac accacccagc ccgacggcaa
     1861 cgccgggccg cggcttgcct gcggaaccat catccgcacg caggggccgc gcaagggagt
     1921 tccgcatcgc gcgcataagt gaggaaattc atgccggtcc t
//
LOCUS       DASWPU010000062         4323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_12703,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000062 DASWPU010000000
VERSION     DASWPU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12703"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VGC27_01310"
     CDS             complement(<1..890)
                     /locus_tag="VGC27_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HEY0281241.1"
                     /translation="MSQQKASRPKARLPRGFRDRGEGEIAAERRLIDTIRTVYESYGF
                     SPLETPAFEYTDALGKFLPDVERPNEGVFSLKDDDEQWLSLRYDLTAPLARYVAENFQ
                     NLPKPFRRYQVGPVWRNEKPGPGRFREFTQFDADTVGSNSPAADAELLMMVCDTLEAL
                     GLKRGEYVVKMNNRKWLDGVLELVGISADDRARRGIVLRAIDKLDRLGFDGVEALLGK
                     GRKDESGDFTKGAELTDEQVYLITTFLGKVRPDLDMKVVEALNKRVDDESTLLGKGVV
                     ELRQIIRILQSNSYGEDRVR"
     gene            complement(1253..3466)
                     /locus_tag="VGC27_01315"
     CDS             complement(1253..3466)
                     /locus_tag="VGC27_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0281242.1"
                     /translation="MAFLSAVHPFSLVRPLLRLLCRVKITGLENLAAAGDRVLIVANH
                     VSVLDAVLLSAFLPGRILFAAPANIAGRRWLKPYWRSGHTLDQTHPITAKMMVEAIKA
                     GRHCMIFPEGRVGRTGGLMKVYESPGMIADKAGAAIVPIRIDGPQYSRFSALGGPVRK
                     ILFPKITLTILPPRKLDLPKEIKGHKRRLLAGSRLHDVMEETLVAGTPVSTLLRQLLA
                     TRAVRGGGHTIIEDAARAPLKHNAFVAGFFTLARLLARELGEREKTVGIMLPNGIPNA
                     TAIFAVQALDRVVAMINFTSGPPRVVQACRMAEIRSVVTLRAFVTAQRLEPVAEALTR
                     ENIRVLYLEDVLARRGAWDRVAGWLRARLPVPLAASGLAKDADGPALLLFTSGTEGAP
                     KGVLLSHRNIIANGFQFGVRIGFSAGDSVFACLPMFHSFGLTLGLFAPLFYGTRTFLY
                     PSPLRYHDIPELVYDTGATLLLGTDTFLGNYARNATPHDFYFLRFAIGGAERIKPETR
                     RIWADKFGVRLFEGYGTTETAPVISVNSPLHYKAGSVGRAVSCLDVKLSPVEGIKDGC
                     ELLVRGLNLMLGYIKADLPGVVQPVTEGWYNTGDVVSLDEAGFITILGRTKRFAKIGG
                     EMVSLGAIEAVVGSLRPNVRHAAVSIPRDRKGETIILLTESDEIELSHLHAPLRAAGL
                     TKLSFPRKVVKVAQIPLLGSCKTDYAAAELLALESEAAAKAGASAVGTDAAGHSE"
BASE COUNT          844 a         1366 c         1330 g          783 t
ORIGIN      
        1 atccgaaccc ggtcttcgcc atacgagttg ctttgaagaa tgcggataat ctgccggagc
       61 tcgaccacgc cttttccaag cagcgtgctt tcgtcgtcga cacgcttgtt gagggcctcg
      121 acgactttca tatcgagatc ggggcggact ttcccaagaa acgtggtgat caaatagacc
      181 tgctcgtccg tcagttccgc gcctttcgtg aagtcgccgg attcgtcttt ccgtcccttc
      241 ccgagcagcg cttcgacgcc atcgaaccca agccggtcga gcttatcgat ggcccggagc
      301 acaatgccgc ggcgcgcgcg gtcgtcggcc gaaatgccga ccaattccag gacgccgtca
      361 agccattttc tgttgttcat cttgacgaca tattcgccgc gcttgaggcc gagcgcctcc
      421 agcgtatcgc agaccatcat cagcagttcg gcgtccgccg cgggagaatt gctgcccacc
      481 gtgtcggcgt cgaactgggt gaattcgcga aagcgccccg gtcccggctt ttcgttgcgc
      541 cacaccggcc ccacctgata gcggcggaag ggcttgggca ggttctggaa attctccgcc
      601 acgtagcgcg ccaagggcgc ggtcaggtcg tagcgcagcg acagccattg ctcgtcgtcg
      661 tccttgagcg agaacacgcc ctcgttcggc cgctccacgt cgggcaggaa cttgcccagc
      721 gcgtcggtat attcgaaggc cggcgtctcc agcggcgaaa agccgtagct ctcgtaaacc
      781 gtgcggatgg tgtcgatcag gcggcgctcc gccgcgatct cgccctcgcc gcggtcgcga
      841 aatccgcgcg gaagccgcgc cttcggacgg gatgcttttt gctgactcat atgtttcacc
      901 cggaatgtgc gggccttgta acggctggat tcccacccgg caaggaaaac gcccgcagcc
      961 cggttcgaga gtccgggagc ggcgcacctg cctcgcttcg ttccatttct ggcgcataac
     1021 catttgaaaa caaaggaaac cgtagaaggt ctgattcggc gctatactgc gtcattgcgc
     1081 tcaggaccct agcgcaacga aggctcgagc gcgccctgcc agccggcggg tacaaacaga
     1141 gggcgaaagc atgcaaaagc ggagccgccc gcgcctgccc gctctggtca ccgtcgaaat
     1201 taggacggcc acctgcccca cgatggcgcc cagtggcgcc gtgccttccc ttctattcgg
     1261 aatggccggc cgcatccgtc ccgacggcgc ttgcgccggc cttcgccgcc gcctcgctct
     1321 caagcgccag aagctccgcg gcggcataat ccgttttgca ggaaccgagc agcggaatct
     1381 gcgccacttt gacgaccttc cgcgggaagg aaagtttggt caaccccgcg gcgcgaagag
     1441 gcgcatgcaa atggcttaat tcgatctcat cggattcggt caacaatatg atggtctcgc
     1501 ccttgcgatc ccgcgggatg ctgacggcgg catggcgcac attcggacgc aacgaaccga
     1561 ccaccgcctc gatggcgccc agagagacca tttcgccgcc gatcttcgcg aagcgtttgg
     1621 tccggccgag gatggtgatg aatcccgcct catcgaggct caccacatcg ccggtattgt
     1681 accagccctc cgtcaccggc tggaccacgc ccggcaggtc cgccttgata tagcccagca
     1741 tcaggttgag accacgcacc aacaactcgc atccgtcctt gatgccttca acaggcgaga
     1801 gtttgacatc gaggcatgag acggcacgcc cgaccgagcc cgctttgtag tgcagcggcg
     1861 agttgacgct gatgacgggc gccgtctcgg tcgtgccgta cccctcgaac agccggacgc
     1921 cgaatttgtc ggcccagatg cgccgagtct cgggcttgat cctttccgcc ccgccgatgg
     1981 cgaaacgcag gaaatagaag tcgtgcggcg tggcgttgcg cgcgtaattg ccgaggaacg
     2041 tatcggtgcc gagcagcaag gtcgcgccgg tgtcgtaaac cagctccgga atatcgtgat
     2101 aacgcagcgg cgacggatag agaaaggtcc gcgtcccgta aaacagcggc gcgaaaagac
     2161 cgagcgtcag accgaaggaa tggaacatcg gcaaacaggc gaagacgctg tcgccggcgc
     2221 tgaaaccgat gcgaacgccg aattgaaacc cgttggcgat gatgttgcgg tgactcagca
     2281 agacgccctt gggcgcccct tcggtgccgg aggtgaacag cagcagggcc gggccgtccg
     2341 cgtccttcgc aaggccgctt gccgcaagcg gcacgggcaa acgcgcccgc agccagccgg
     2401 caacgcgatc ccaagcgccc ctccgggcca gaacatcctc caaatagagg acgcggatgt
     2461 tctcccgtgt caaagcctcg gcgaccggct ccaggcgctg ggccgtgacg aaggcgcgaa
     2521 gcgtgacgac gctgcggatc tcggccatgc ggcaggcctg caccacgcgc ggcgggccgg
     2581 aggtgaaatt tatcatggcg acgacgcgat ccagcgcctg aacggcgaag atcgccgtgg
     2641 cgttagggat gccgttcggc agcatgatgc cgacggtctt ctcccgttcg ccgagctcgc
     2701 gcgccagcaa tcgcgccaga gtgaagaacc cggcgacgaa ggcattgtgt ttcaacggcg
     2761 cacgcgcggc gtcctcgatg atcgtatggc cgccgccgcg taccgcgcgc gtcgccagca
     2821 actggcgcag caaggtggag acgggggtgc ccgccaccag cgtctcttcc atcacatcgt
     2881 gcagccgcga tccggcgagc aggcggcgct tatgaccctt gatctctttc ggcaggtcca
     2941 gcttgcgcgg tggcaggatg gtcagggtga tcttgggaaa cagaattttg cgcaccggcc
     3001 cgccgagggc ggagaaccgg gaatattgcg gcccgtcgat gcggatgggc acgatcgccg
     3061 cccccgcctt gtcggcgatc atgccggggc tctcatagac cttcatcagg ccgccggtgc
     3121 ggccgacgcg gccttcggga aagatcatgc aatgcctgcc ggccttgatg gcctccacca
     3181 tcattttggc ggtgatcggg tgcgtttgat ccagcgtgtg gccgctgcgc caatagggtt
     3241 tcagccagcg gcgccccgca atattggccg gggccgcgaa cagaatgcgc ccgggcagga
     3301 aggcggacag caagaccgca tccagcaccg aaacatggtt ggcgacgatc agcacgcggt
     3361 ctccggcggc ggcgaggttc tccaggccgg ttatcttgac gcggcacagc agacgcagca
     3421 gcggccgcac aagggaaaaa gggtgaaccg cggaaagaaa agccatgaca atctcacgac
     3481 gaccaaaagg gacacgggat caggattccg gaaggtccgc tcgtctcgga attgcgagcc
     3541 ggccacgaca aacccgtcat gcgggaaaca ctccagaacg cacctatggc actttttgta
     3601 ctcaagctcg agcaaaagac cccgctcagg ccgcggctgc gacactgtgc cgaacggtcc
     3661 gcccgcggcc cgggcatgcg ggcaaagccg gccggcatcc cggtacgcgg cactgaacca
     3721 ctaaggctgc cgtgaagccg aagatcgtgc cgcttggccc aaaggccccg gcggaactgc
     3781 gccgcgcatt tccaccgtgg cgcattcccc gccacgactt cgccgccatc gcaaaacgcg
     3841 cgcggtgaaa ttcgattgaa tttggaagca gccgacttcg tatcggcgat ctgccgcagc
     3901 gttcgcgccg tccgtgtaaa gacgttttgc tcgctttagc gaacgcgcca tcgcaaggcg
     3961 ggcgttgcgg acaccttatt atacatcccg gttttggatt tcgatctgcg tttaccaacg
     4021 acgcgatttt ctagcggcac ggtgagccaa agcggataac atcgtcatgg cgtgatgtga
     4081 tccgcgacaa caaaagcatt ttttgcatgt tgtttggagg cattttgacg cacaactaag
     4141 tcgggtgtat accctggttt cattgccgga ggttgagtca gcaatgtgtg gtcatagata
     4201 tcctcagttg cacgccgtct tcttcgtttt tggaatgagt tgatccagtt tctgtatggc
     4261 gcatttcacg ggtgagtctc tcggattgcc gacaattcgt aggagcactc atggcgcgat
     4321 atc
//
LOCUS       DASWPU010000063        11340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_55561,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000063 DASWPU010000000
VERSION     DASWPU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55561"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VGC27_01320"
     CDS             complement(<1..504)
                     /locus_tag="VGC27_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281243.1"
                     /translation="MIGGFPRLLVMVCAMAALAIFARADAQTAKGPTPPAVLTADQDH
                     QRLMDLLHIMTLRPGANGSDPASPNAANYDEARANPYPVLPDPLKLNNGKPVTTAAQW
                     WSERRPQIVADFDREMYGRVPTKLPAVRWELTGTTSATIGTQSAVTKRLLGHVDNSSY
                     PPVKVGIA"
     gene            complement(515..2029)
                     /locus_tag="VGC27_01325"
     CDS             complement(515..2029)
                     /locus_tag="VGC27_01325"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0281244.1"
                     /translation="MHAADNAAIVRGELTVHADKPLAVINPDVYGQFAEHLGNGIYGG
                     LWVGEDSKIPNLRGFRKDVVGALKKLDVPVVRWPGGCFADQYHWRDGIGPRAKRPVRV
                     NTTWGGVTETNEVGTHEYFELMEQLGSKSYINGNVGTGSPQETKDWLEYLTSDSKSSL
                     ALERAKNGRVKPWKIDYFAFGNELWGCGGNMRPDFYADLYHWYAAFAKTPADNTPLWI
                     AAGATDGDTAWNEALMATNKFKDMNAVTMHHFSLPTRSWTGPKGPATGFGEDQWMSIM
                     QQALQIDGMIAAHSAMMDKYDPQKNVGLFVDEWSNWYDVEPGTNPGFLHQQNTLRDAV
                     TAAVNLDIFQSHADRVRMACITQMVNVLQAMILTDGPRMLLTPTYHVYMMYKPFRGAS
                     ALAIDLKTPDYRFGDAAVPALHASAARRADGTLVISLVDLDPNRPAHVAVSLAGAGAK
                     SVTGTILTADAMDAHNSFDHPNAVEPAVFTGATLQDGVLNVDLPAKSVVVLTLQ"
     gene            2300..5152
                     /locus_tag="VGC27_01330"
     CDS             2300..5152
                     /locus_tag="VGC27_01330"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HEY0281245.1"
                     /translation="MAAPASAAPSLTLSDGKSVAAIVYDTADSAPIAKAAELLAHDLK
                     ARSGFAPVIATHFNGPGPAILIGRSDSPLIASLLKANKIDTAPLAGKWETYGRAVVPA
                     PWNPQQSVLVIFGSDTRGTIWGVIDLTREIGVSAWEWWADVKILKADRIAVDAALRTS
                     KEPSVKYRGIFLNDEEYGLFPWASMTYDPKLGNIGPKTYARIYELMWRLKANTFWPAM
                     HTVTEAFNKVPGNPEMADAYAMIHASSHVEMMLRSNPREWDEKTMGPYNFVTNRENML
                     KYWDEAVQKFGKYENVYTVGLRGADDYPMAGADTPQGMARILEDVFAEQRKILSNRLG
                     KPADQIPQVFTPYKEVLPAYDAGLKVPDDVTLAWPDDNFGYIRRLSNAEERKRAGGAG
                     VYYHISYWGGPMSYLWLASTHPALLWEEMNKAYRFEARRYWILNVGDIKPGEYLTQLF
                     MDIGFDAEAFPDPASVRAHLKNWAAENFGAANADEIADIMWRTYDLAFGRNPEFMAWS
                     TAFPETAVSQTKFNMLDFGDENARRAEAYRAVMASAQALAAKLPDDLKPAYYQLVQYP
                     VEAAGNMNIRQLSLDKTITYGLQRRESANSYSAEAKRAQATIEAGAHYYNNVMLDGKW
                     RGMMGIRPHDLPAYDSPHLPVWDNHGDKSCGWQTEGGGYFAGGGWPQRLATFQRAVPR
                     QRYLDFFVTGPVAANWTLKPTVPWITLSQSSGSLGPQRLEQRVWIGIDWAKAPDGATA
                     GIVASCGETKAEMPITVRTAPANTVKDVSFLEDNGIVSIYAVHADEKSDGWEVLDGLG
                     HTGASLRSDLGLASVDAGNPAAIAKAPHVTYRFATATKRGYDLVQYAQAKLRVIALPI
                     LPITSDNGMRVAVSVDGGPAKVLDFYAAEFTEPWRQHVLTNAAVETVNALDLKPGAHT
                     LTVYALDPGVILDRFEIAFDEAPRAYGPVPETRIAQ"
     gene            5149..6402
                     /locus_tag="VGC27_01335"
     CDS             5149..6402
                     /locus_tag="VGC27_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEY0281246.1"
                     /translation="MSKLSRRDWMITAAAAAGGMSFGQALGAAKRPNEPGRYAGREPG
                     GSAPDSLNGIARTKGLRFGSCIGTGPTKGELELLRPEVLARPRRASAYEDTQMRVLTA
                     SQCGIVVPENELKFYVLRPRPGVFDFRRADMLMDFASRNHLAMRGHTLFWNRTNWTPR
                     WVNELDFGSRPATAAEKMLREHIFKVCRHYGERIFSYDVINETVNQDTGELEDTVFTK
                     HLGWNVIDICFHAAREAAPHAEMVYNDFPSWGPDGTHRAGLLKLLDYTKKKNLPVDTF
                     GIQAHIGFGNTGGGPGAERDETAWRKFLDEVTAMGFALIITEFDVNERYTQGDVAARD
                     RIVADLGKTYLDIILSYPQLRYVMAWGLVDKYSWLQEFTPRADGLPQRCTPYDDNYQP
                     KPLREAIAAAFAAAPARTEMKIEPA"
     gene            6486..7706
                     /locus_tag="VGC27_01340"
     CDS             6486..7706
                     /locus_tag="VGC27_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEY0281247.1"
                     /translation="MNKFTRRDCMVLGLAATAAGCAPLDSVTNSLTPAPAAGSAPDSL
                     NGLAKANGLLFGNCLGTGPSGAPTRPGAPPDTRANQFDDPDMRALMIAQCGILVPENE
                     LKWHSLRKTPEGFDFARADKLVDFAGESGLAVRGHTLFWARPQNIPGWVADYDFGANP
                     RAAAEAMLVEHVKTVCGRYGKRIFSYDVINELIVPETGELEQSAFTKYLGPDVVDVMF
                     HAAHEAAPHAQLVYNDYMSWEPKSEKHRATVLKLLERMKKNNVPVDALGIQAHIGSSP
                     TGTAEGLADTDFQEWRKFLDDVTAMGIDLIVTEFDVNDKYVEGDIAKRDRVVADYGKS
                     YLDVALSYPQLRYVMAWGIVDKYSWLQRTSPRPDGLPKRPCPYDDNFQPKLLREVMAE
                     AFRTAPARPAVKVG"
     gene            7720..9090
                     /locus_tag="VGC27_01345"
     CDS             7720..9090
                     /locus_tag="VGC27_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0281248.1"
                     /translation="MNWDSNRRQVLSGAVAAAAAASLGGAAFAKARPYRVGVVGAGWM
                     GKANVFALMQVAPVEVVALCDVDRVMLEEARKMIVAFPDSVAPQSRRPEIYRDYRTMF
                     AKHRFDIVIIGTPDHWHALPALAAIKAGAHVYLEKPVALDVVECQALVAAARAGNRVV
                     QVGTQRRASPPHIEARDRVLREGRLGQIGYVEIFGYSRQRAKSFPPPSPPPSTLDWDF
                     YCGPAPLVAYNPGIHPLNWRAFREFGNGHIGDLGVHLIDTCRWMLDLGWPKKISSAGG
                     VFVNTDSISTQPDTQTAQFEYDNLLMNWTSRQWGTVPEGPAGGWGAHIYGDKGTLRLG
                     SISYVFEPAPGQGEPLSGNLDAETGKFPNDKLLPPNFHQLVPLTRPNMRDFIAAIEAG
                     RKPVADIEEGYISTSSIILANIAMDLGRPVRWDAQQQRVTGDDEAQKRLARPYRAPWV
                     HPGAAV"
     gene            9128..10420
                     /locus_tag="VGC27_01350"
     CDS             9128..10420
                     /locus_tag="VGC27_01350"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0281249.1"
                     /translation="MDRCKAFILIAAVSLGGCGTTGDASQSNGGNGVDPATVDTVLKT
                     FIDNGSLVGVSALVHKDGKEAYFGAFGLADREAGRPMARDTIVQIFSMTKPVTGVALM
                     TLWEQGKFRLDDPVAKYIPELADVRVYAGTDAAGNIVTVPALRPITIRDLTRHTAGLT
                     YCGNDNLPALKPGCDAANPTALDNTLDDFARKLGTLPLEFQPGEKWLYSYAVDVQAVL
                     VQRLSGEPFDKYIREHVLTPLGMTHTGFFVAPDQRDKLAAIYDWHPDGSLTRQPDEKA
                     FRLNTNHWPLTPGGHGLTSTLDDYSRLTRMLLNGGELDGVRVLHPDTVRMMSTSQLDP
                     AVTDRAWLVADKGEVGFGIDFAVRLYPPQKADGRAGEAGEFFWDGFANTLFWVDPKNH
                     LTAVLFTQYQPFGKVPLHKAFRDAIYSSVDKSAMPPQE"
     gene            10475..>11340
                     /locus_tag="VGC27_01355"
     CDS             10475..>11340
                     /locus_tag="VGC27_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HEY0281250.1"
                     /translation="MKFLPALRSLAALIALCCFASAAHAEDGYDLWLRYHPVEKASLA
                     KYRAAAAVLVTTGTSPTIQAARDELVRGLSGLLARTAPVDDTIRGNDAILFGTPANSP
                     TIAGLNLPLAKLGADGYVIKSVTAKGRKLTVIAANNDIGVLYGAFHYLRLIQTGQPVD
                     DLDISSAPLIQNRILNHWDNLDRNIERGYSGFSIWNWPKLPGYLDPRYTDYARAQASI
                     GVNGVVLNNVGAQAAILTPLYLKKAAALADVFRPYGVKIYFSARFSAPIELGGLKTAD
                     PLDPAVAAWWKA"
BASE COUNT         2124 a         3637 c         3573 g         2006 t
ORIGIN      
        1 agcgatgccg accttcaccg ggggatagga cgaattatcg acgtgaccca gcagacgttt
       61 ggtgacggcg gattgcgttc cgatcgtcgc gctggtcgtc cccgtcagtt cccagcggac
      121 ggcgggcagc ttggtgggca cgcggccgta catctcgcgg tcgaaatccg cgacgatctg
      181 agggcgccgt tcgctccacc attgcgccgc cgttgtcacc ggcttgccgt tgttcagctt
      241 caagggatcg ggcagcacgg gataaggatt ggcgcgcgct tcgtcgtaat tggccgcgtt
      301 gggcgacgcg ggatcgctgc cgttcgcgcc ggggcgcagc gtcatgatgt gcaacaggtc
      361 catcaggcgt tgatggtcct gatctgcggt cagcacggcg ggaggcgtcg gacctttggc
      421 cgtctgcgcg tcggcgcgcg caaaaatcgc cagcgccgcc atcgcgcaca ccatcacgag
      481 aagccgaggg aagccgccga tcatcttccc ggcctcattg cagcgtgagc acgacgaccg
      541 atttcgccgg caggtccacg ttcagcacgc cgtcctgcag cgtggcgccg gtgaagacgg
      601 caggctcgac cgcgttcgga tgatcgaagc tgttgtgcgc gtccatggcg tccgccgtca
      661 gaatcgtccc cgtcaccgat ttcgcccccg cacccgcaag cgagaccgcg acatgcgccg
      721 ggcggttcgg atcgagatcg accagcgaga tcaccagcgt gccgtcggcg cggcgcgcgg
      781 ccgaggcgtg cagcgcgggc acggccgcat cgccgaagcg ataatccggc gtcttcagat
      841 cgatcgccag ggcggacgcc ccacggaacg gcttgtacat catgtaaacg tgataggtcg
      901 gcgtcagcag catgcgcggg ccgtcggtga ggatcatcgc ttgcagcaca ttgaccatct
      961 gggtgatgca ggccatgcgc acgcggtcgg catgggattg gaaaatgtcg agattgacgg
     1021 ccgccgtgac ggcgtcgcgc agcgtgttct gctgatgcag gaagccggga ttggttccgg
     1081 gttcgacatc gtaccaattg ctccattcgt cgacgaagag accgacgttc ttctgcggat
     1141 cgtacttgtc catcatggcg ctatgggccg cgatcatgcc gtcgatctgc aacgcctgct
     1201 gcatgatgct catccactgg tcctcgccga agccggtggc gggacctttg ggtccggtcc
     1261 agcttcgggt cggcagcgag aaatgatgca tcgtgacggc gttcatgtcc ttgaacttgt
     1321 tggtggccat caaggcttcg ttccacgccg tatcgccatc ggtggcgccg gccgcgatcc
     1381 atagcggcgt gttgtccgcg ggcgttttgg cgaaggccgc gtaccagtga tagagatcgg
     1441 catagaagtc cggccgcatg ttgccgccgc agccccacaa ctcgttgccg aacgcgaaat
     1501 agtcgatttt ccacggcttc acgcgcccgt tcttggcgcg ctccagcgcc agcgacgatt
     1561 tggaatccga ggtcagatat tcgagccagt ccttggtttc ctgcggcgag ccggtgccga
     1621 cattgccgtt gatgtacgac ttggagccca gttgctccat caactcgaaa tattcgtggg
     1681 tgccgacttc gttggtttcc gtcacgccgc cccaagtcgt attgacgcgg acggggcgct
     1741 tggcgcgtgg gccgatgccg tcgcgccaat gatattggtc ggcgaagcag ccgcccggcc
     1801 agcgcacgac cggcacatcg agtttcttca gggcgccgac gacgtccttg cgaaaaccgc
     1861 gcaggttcgg aatcttggag tcctcgccga cccacagccc gccatatatg ccgttgccga
     1921 gatgctcggc gaattgtccg tagacatcgg gattgatgac ggccagcggc ttgtcggcat
     1981 ggacggtcaa ttcgccccgc acgattgccg cgttatctgc tgcatgcatg tcgctttgcg
     2041 ggacgaacag gagcaagata aaggcgaatg gaatcatgca aaaaaacatc cggtggcgat
     2101 tgtgtttcat cgacgtctcc tctttgccgc gtccgacttg agttgaaccg gaagccgaaa
     2161 aatggtagcg ctcccgcgcg ggggggccaa aggaaaaaga caagagcgga ggaccccgat
     2221 gcggaatatc catggtgcga tgcagcggac aagagcatgt cggtggaaag ctctgtttgg
     2281 tattttggct gctctcgcct tggccgctcc tgcttccgcg gcgccctctc tgaccttgtc
     2341 cgacggcaaa tcggtcgccg ccatcgtcta tgatacggcc gatagcgctc ccatcgccaa
     2401 ggccgcagaa ctgctggcgc acgatctgaa agcgcgtagc gggtttgcgc ctgttattgc
     2461 gacgcacttc aacgggcccg gccccgcgat cctgatcggc cgcagcgatt cgcctttgat
     2521 cgcttccctg ctcaaggcca acaaaatcga tacggcgccg ctcgcgggca agtgggagac
     2581 ttatggacgc gccgtcgttc ccgcgccgtg gaacccgcaa caatccgtgc tggtgatttt
     2641 cggctccgat acgcgcggca cgatttgggg cgtgatcgat ctgacgcggg aaatcggcgt
     2701 ctccgcctgg gaatggtggg ccgacgtgaa gattctcaag gcggaccgca tcgccgtcga
     2761 tgccgctctg cgcacttcga aagagccgtc ggtcaaatac cggggcatct tcctcaacga
     2821 cgaggagtat ggcctgtttc cctgggcatc gatgacctac gaccccaagt tgggcaatat
     2881 cggccccaag acctatgcgc gcatctacga gttgatgtgg cggctgaaag ccaacacctt
     2941 ctggcccgcc atgcacaccg tgaccgaggc gttcaacaag gtgccgggca atccggaaat
     3001 ggcggacgca tacgccatga tccatgccag ctcccatgtc gagatgatgc tgcggagcaa
     3061 cccgcgcgaa tgggacgaaa agacgatggg tccctacaac ttcgtcacca accgggaaaa
     3121 catgctgaag tactgggacg aggcggtgca gaagttcggc aaatacgaga acgtctacac
     3181 ggtgggattg cgcggcgcgg acgactatcc catggcgggc gccgatacgc ctcagggcat
     3241 ggccaggatc ctggaagacg ttttcgccga acagcggaaa attctgtcga accggctggg
     3301 caaacccgcc gaccagattc cgcaggtgtt cacgccctat aaggaagtgc tgccggccta
     3361 tgatgcgggc ctcaaagtgc ccgacgacgt tacgctggcc tggcccgacg acaatttcgg
     3421 ctacatccgc cggctgagca atgcggaaga acgcaagcgc gcgggcggcg cgggcgttta
     3481 ttaccacatc tcctattggg gcgggccgat gtcctatctc tggctcgcct ccacccatcc
     3541 cgcgctcttg tgggaggaga tgaacaaggc ataccgcttc gaggcgcggc gctattggat
     3601 tctcaatgtc ggcgacatca agccgggcga atatctgacg caactcttca tggatatcgg
     3661 cttcgacgcc gaggcgtttc cggacccggc gagtgtgcgg gcgcatctga agaactgggc
     3721 ggcggagaat ttcggcgcgg ccaacgccga cgagatcgcc gatatcatgt ggcgcactta
     3781 cgacctcgcc ttcggccgca acccggaatt catggcctgg tccacggcct tcccggagac
     3841 ggcggtgtcg caaaccaaat tcaacatgct cgacttcggc gatgaaaacg cgcgccgggc
     3901 ggaggcctat cgcgccgtca tggcaagcgc ccaggcgctg gcggcgaagc tgccggatga
     3961 cctcaagccc gcctactatc agctcgtgca atatccggtg gaagcggcgg gcaacatgaa
     4021 catccgccag ctctcgctcg acaagacgat tacctacggc ttgcagcgcc gcgaaagcgc
     4081 caacagctac agcgccgaag ccaaacgggc gcaggcgacc atcgaagccg gggcgcacta
     4141 ttacaacaat gtgatgctgg acggaaaatg gcgcggcatg atgggcatcc gtccgcacga
     4201 cctgccggcc tacgactcgc cgcatctgcc ggtgtgggac aaccatggcg ataaatcctg
     4261 cggctggcag accgagggtg gcggctattt cgcgggcggc ggatggccgc agaggctcgc
     4321 caccttccag cgggcggtgc cgcgccaacg ctatcttgat ttcttcgtca ccggaccggt
     4381 cgccgccaat tggacgctga agccaaccgt gccctggatc acgctgagcc aaagctccgg
     4441 cagcctcggt ccgcagcggc ttgagcagcg cgtctggatc ggcatcgact gggccaaggc
     4501 gccggacggg gccaccgccg gcatcgtggc gagctgcggc gaaaccaagg cggagatgcc
     4561 gatcacggtg cgcacggcgc cggccaatac ggtgaaagac gtttccttcc tcgaagataa
     4621 cggcatcgtc tccatctacg cggtccatgc ggacgagaag agcgacggct gggaggtgct
     4681 ggacgggctg gggcataccg gcgccagcct gcgcagcgat ctgggcctgg cctcggtcga
     4741 tgcgggcaac cctgccgcga tcgccaaggc gccgcacgtc acctatcgct tcgcgacggc
     4801 cacaaagcgg ggctacgact tggtgcagta cgcacaagcg aagctgcgcg tgatcgccct
     4861 gccgatcctg ccgatcacca gcgacaatgg catgcgcgtg gcggtttcgg tcgacggcgg
     4921 gccggccaag gtcctcgact tctatgccgc cgagttcacc gagccgtggc gtcagcacgt
     4981 gctgaccaat gcggcggtcg agaccgtaaa cgcccttgac ctcaagcccg gcgcgcacac
     5041 actgacggtt tacgccttgg atcccggggt catcctcgac cgcttcgaga tcgccttcga
     5101 cgaggcgccg cgtgcctatg gacccgttcc ggaaacaagg attgcgcaat gagcaaacta
     5161 tcacgacgcg actggatgat aacggcggcg gcagcggcgg gcggcatgtc ttttgggcaa
     5221 gcgctcggcg ccgcgaagcg gccgaatgag ccgggccgct atgccggccg cgaacccgga
     5281 ggcagtgcgc ccgactcgct caacggcatt gcgcggacca aagggctgcg cttcggaagc
     5341 tgcatcggca ccggccccac caagggagag ctggaactcc tgcggccgga ggtgctggcg
     5401 aggccgcggc gcgcgtctgc ttacgaggat acgcagatgc gcgtcctcac ggcatcccaa
     5461 tgcggcatcg tggtgcccga gaacgaactc aaattctatg tgctgcggcc gcgccctgga
     5521 gtgttcgatt tccgccgtgc ggacatgctg atggatttcg ccagccgcaa tcacctggcg
     5581 atgcgcggcc acaccctgtt ttggaaccgc acaaattgga cgccgcgctg ggtcaacgaa
     5641 ctcgatttcg gctcccgtcc ggcaacggcg gcggaaaaga tgctccgcga acacatcttc
     5701 aaggtctgcc gccactatgg cgagcgcatt ttcagctacg acgtgatcaa cgagaccgtc
     5761 aaccaggaca ccggagagct ggaagacacg gtcttcacca agcatctggg gtggaacgtc
     5821 atcgacatct gcttccatgc ggcgcgcgaa gcggcgcctc atgcggagat ggtctacaac
     5881 gatttcccca gttgggggcc cgacggcacg caccgcgcgg gtttgctcaa gctgctggac
     5941 tatacgaaga agaagaacct gcccgtcgat acgttcggaa tccaggccca tatcggcttc
     6001 ggcaataccg gcggcggccc cggcgccgag cgggacgaaa cggcctggcg caagttcctc
     6061 gacgaggtga ccgccatggg cttcgccctc atcatcacgg aattcgacgt caacgaaagg
     6121 tacacgcagg gtgacgtggc cgcgcgcgac cgtatcgtgg ccgatctggg caaaacctat
     6181 ctcgacatca tattgagcta tccgcaactg cgttacgtga tggcttgggg cttggtggac
     6241 aagtactcct ggttgcagga gtttacgccg cgcgccgacg gcctgcccca gcgttgcacg
     6301 ccttatgatg ataactatca gccgaagccg ctgcgcgagg cgatcgcggc ggcgttcgcg
     6361 gcggcgccgg caagaacgga gatgaagata gaacccgcat gacgagtgga agacagagcc
     6421 ctctggcagg cgcataattc tgtgcggccg gtttccgcgc cagttcctaa aacgaggatg
     6481 acagaatgaa caaattcacg cgacgcgact gcatggtact cggactggcc gcgacggcgg
     6541 ccggctgcgc gccactcgac tctgtgacga actcccttac ccctgcgccg gcggccggct
     6601 cggcgccgga ttcgttgaac ggactggcga aggccaatgg gcttcttttc ggaaactgcc
     6661 tgggcaccgg tccgagcggc gcccccaccc gtccgggcgc tccccccgac acacgcgcca
     6721 accagttcga tgacccggac atgcgcgcac tgatgatcgc ccaatgcggc atcctggtcc
     6781 ccgaaaacga gcttaaatgg cactcgctgc gcaaaacgcc ggagggcttc gacttcgcgc
     6841 gcgccgacaa gcttgtcgat ttcgccgggg aaagcggcct ggcggtgcgc ggccacacct
     6901 tgttttgggc ccgtccgcaa aacatacccg gttgggtggc agactacgat ttcggcgcca
     6961 atccgcgcgc cgcggccgag gccatgctgg tcgagcacgt caagacggta tgcgggcgtt
     7021 acggcaaacg tattttcagc tatgacgtga tcaacgaatt gatcgtcccc gaaacgggcg
     7081 agctggagca gagcgccttc accaaatatc tggggccgga cgtggtggac gtcatgttcc
     7141 acgccgcgca tgaagcggcg cctcatgccc agcttgtcta taacgactat atgagctggg
     7201 agccgaagag cgagaagcat cgcgccaccg tgctcaagct tctcgaacgg atgaagaaga
     7261 acaacgtgcc cgtcgatgcg cttggcatcc aggcccatat cggctccagc cccacgggta
     7321 ctgcggaagg gttggcggac accgactttc aggaatggcg caaatttctc gacgacgtga
     7381 cggcgatggg aatcgacctc atcgttaccg aattcgacgt caacgacaaa tatgtggaag
     7441 gcgatattgc caaacgcgac cgcgtcgtcg ccgattacgg caagtcgtac ctcgatgtgg
     7501 ccctcagcta tccccaactg cgctatgtga tggcctgggg aatcgtggac aaatattcgt
     7561 ggctgcaacg cacttcgccg cgcccggacg ggttgcccaa gcggccgtgt ccgtacgacg
     7621 acaatttcca gcccaagctg ctgcgcgagg tcatggccga agctttccgc accgcgcccg
     7681 cgcgccccgc cgtgaaagtc ggttgatagg gagacgccta tgaactggga ttcgaatcga
     7741 cgccaagtgc tgagcggggc ggtcgccgcc gcggcggcgg cgagcctggg cggcgccgcg
     7801 ttcgccaagg cgcggcccta ccgggtcggc gtggtgggcg ccggctggat gggcaaggcc
     7861 aatgtctttg ctctcatgca ggtcgcgccc gtcgaagtgg tcgccctgtg cgacgtagac
     7921 cgcgtcatgc tcgaagaggc gcggaagatg atcgtggcgt ttcccgattc cgttgcgccc
     7981 cagagccggc gcccggagat ttatcgcgac tatcgcacca tgttcgcgaa gcatcgcttc
     8041 gacatcgtga ttatcgggac gcccgatcac tggcacgccc ttccggcgtt ggcggcgatc
     8101 aaggccggcg cgcatgtcta tctggaaaag ccggttgcgt tggatgtcgt cgagtgccag
     8161 gccctggtcg ccgccgcgcg cgcgggcaac cgggtggttc aggtcggcac ccagcgccgc
     8221 gcctcgcccc cgcacatcga ggcgcgcgac cgcgttctgc gcgaaggccg gctcggccag
     8281 atcggatatg tcgagatatt cggctactcc cgccagcgcg caaaatcctt tccgccgccg
     8341 tcgccgccgc cctccacgct cgattgggat ttctactgcg gcccggcgcc gctggtcgcc
     8401 tacaaccccg gcatccatcc tttgaactgg cgcgcgttcc gcgaattcgg caacggccac
     8461 atcggcgatc tcggcgttca tctcatcgat acctgccgct ggatgctgga cctgggctgg
     8521 cccaagaaaa tttcgtcggc cggcggcgtc ttcgtgaaca cggacagcat ttccactcag
     8581 cccgatacgc agacggccca gttcgagtac gacaatctcc tgatgaactg gacgagccgc
     8641 caatggggta ccgtccccga ggggccggcc ggcggctggg gcgcgcacat ctacggcgac
     8701 aaaggcacgc tcaggctcgg ctcgatcagc tatgtgttcg agcccgcgcc ggggcaaggc
     8761 gaaccgctgt cgggcaatct cgacgcggag acgggcaagt tccccaatga caagctgctg
     8821 ccgccgaact ttcaccagct cgtgccgctg acgcggccca acatgcgcga cttcatcgcc
     8881 gccatcgaag ccggcaggaa gccggtggcc gacatcgagg aagggtatat ctccacctcg
     8941 agcatcatcc tggccaacat cgcgatggac ttgggccgtc cggtccgctg ggacgcacag
     9001 cagcagcgcg tgaccggcga cgacgaggcg cagaagcggc tggcgcggcc ctatcgtgcg
     9061 ccatgggtcc acccgggggc ggcagtttaa acagcgtgcg catcatcttt tcgattgggg
     9121 agacgagatg gaccggtgca aagcttttat actgatcgcg gcggtctcgc tcggcggctg
     9181 cggcacgacc ggcgatgcgt cgcagtccaa tggcggcaat ggcgtcgatc ccgccacagt
     9241 cgacacggta ctgaagacgt ttatcgacaa cggatcgctt gtcggggttt cggcgcttgt
     9301 ccacaaggat ggcaaggaag cctatttcgg cgccttcggc ttggccgacc gcgaggcggg
     9361 gcgccccatg gcgcgcgata cgatcgtgca gatattttcg atgaccaagc ccgtcaccgg
     9421 cgtcgcattg atgacgctgt gggagcaggg caagttccgg ctcgacgatc cggtcgcgaa
     9481 gtacattccg gaactggccg atgtgagggt ctatgccggc accgatgcgg ccggaaacat
     9541 cgtgacggta cccgcgctgc ggccgatcac catccgcgat ctgacgcgcc acacggcggg
     9601 ccttacctat tgcggcaacg acaatcttcc ggcgctcaag ccgggctgcg acgccgccaa
     9661 tcccacggcc ctcgacaaca cgctcgacga ctttgcgcgg aagctgggaa cgctgccgct
     9721 cgaattccag cccggcgaga aatggctgta cagctatgcc gtggacgtcc aggcggttct
     9781 ggtgcagcgg ctgtcgggcg aacccttcga caagtacatc cgggaacacg tcctcacgcc
     9841 tttgggcatg acccataccg gcttcttcgt ggcgccggac cagcgcgaca agcttgcggc
     9901 gatctacgat tggcatcccg acggcagcct cacgcggcag cccgacgaga aggcgttccg
     9961 gttgaacacc aaccattggc cgctgacgcc tgggggccac ggcctgacct cgacgctcga
    10021 cgattattcc cgcctcaccc gcatgctgct gaacggcggc gagcttgacg gcgtgcgcgt
    10081 cctgcatccc gacacggtgc gaatgatgtc caccagccag cttgacccgg ccgtaaccga
    10141 ccgtgcctgg ctcgtggccg ataaaggcga agtcggtttc ggcatcgatt tcgccgtccg
    10201 gctttacccg ccgcagaagg ccgacggcag ggcgggcgaa gcaggcgagt tcttctggga
    10261 cggcttcgcc aacacgctgt tctgggtgga tccgaagaac catctgaccg ccgtgctttt
    10321 cacccaatac cagccgttcg gaaaggtccc gctgcacaag gctttccgcg atgcaatcta
    10381 cagctcggtc gacaagtccg cgatgccgcc gcaagagtga gacgcgcttg tcgttccgat
    10441 tccgtccgtt cctcgaacaa gctgaggtcc ctccatgaaa ttccttcccg ccctgcgcag
    10501 cctggccgcg ctgatcgcgc tatgctgctt cgcctctgcc gcccacgcgg aagacggcta
    10561 cgatctatgg ctccgctatc atccggtgga aaaggcgtcg ctcgcgaagt atcgggccgc
    10621 cgctgcggtt ctggtcacta ccggaacctc gcccacgatc caagccgcgc gcgacgagct
    10681 tgtccgcggt ttgagcgggc tgctggcccg caccgcgccg gttgacgaca cgatccgcgg
    10741 caacgacgcg atcctgttcg gcactccggc caactcgccg accatcgccg gcctcaatct
    10801 gccgctcgcc aaacttgggg cggacggtta cgtcatcaag agcgtgaccg ccaaaggccg
    10861 caagctgacc gtcatcgccg ccaacaacga tatcggcgtt ctctacgggg ccttccatta
    10921 cctgcgcctg atacaaaccg gccagccggt cgatgatctc gacatctcct ccgcgccgct
    10981 aatccagaac cgaatcctca atcattggga caatctcgac cgcaacatcg agcgcggtta
    11041 ttcgggcttc tcgatctgga attggccgaa gctgcccggc tatctcgacc cgcgctacac
    11101 cgactatgcc cgcgcccaag cctccatcgg cgtcaacggc gtcgtcctca acaatgtcgg
    11161 cgcccaggcg gcgatcctga cgccgctcta tctgaagaag gcggccgcgc tggccgatgt
    11221 cttccgtccc tacggggtca agatctattt ctcggcgcgg ttcagcgccc ccatcgaact
    11281 gggcggcctc aagaccgccg acccgctcga tccggcggtg gcggcgtggt ggaaggccaa
//
LOCUS       DASWPU010000064         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_31058,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000064 DASWPU010000000
VERSION     DASWPU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31058"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VGC27_01360"
     CDS             <1..632
                     /locus_tag="VGC27_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281251.1"
                     /translation="DGQNGVVGSMYVIWSGCLLFVTSGTSQQIAGVLFVLSLVFLVFN
                     LRGKLFLGDCGSYGVTFAVGLLVTLAHAKAQVSLETVIVWFFIPVADCIRLLISRPLR
                     GVSPFMGDHDHFHHRLEDKLGKNQGLATYAVVIAASSLIATLDPRFALVCLCALSAFY
                     FSFAWLSDATTVSVSKPESESDEVTATAGNVVPLTGDSAGDRKRQRTTH"
     gene            709..1545
                     /locus_tag="VGC27_01365"
     CDS             709..1545
                     /locus_tag="VGC27_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281252.1"
                     /translation="MAHVSVKDTLEFSFSFLFRNAARIAARIALPALGGWFTFYIAFF
                     LYLTELERYLGNPSDRVASLVLGLATAGLLVTLFMHAIIVSAIASLALGLEAPGWKYF
                     SIGRRAWRLYAADLRCVLVFAAFIAAVRLGEAAIGRLGVMPGQVWIADLVMGVGLYWL
                     FVRFGFLVAPIAVARRDGEILRRGWRLSSGHFWSIKAITAVLALPGFAIELAGEFLLR
                     LLGTFPELGATGSMASLVALYREILPAVLIAIGVGYLVAVTLLTLARVSVYRQLMDQP
                     ET"
     gene            1559..>1750
                     /locus_tag="VGC27_01370"
     CDS             1559..>1750
                     /locus_tag="VGC27_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEY0281253.1"
                     /translation="MHVGQFDFDLPEELIALRPARPRDAARLLVVHGDGRLEHALVGD
                     LGRFLRAGDALVLNDTRVIR"
BASE COUNT          243 a          536 c          548 g          423 t
ORIGIN      
        1 ccgacggtca aaacggcgtt gtcggcagca tgtacgtcat ttggtcgggc tgtctgctgt
       61 tcgtcaccag cggcacgagc cagcagattg cgggcgtgct gttcgtcctg tcgctggtgt
      121 ttctggtttt caatctcagg ggaaagctct ttctcggcga ttgcggctcc tacggcgtca
      181 ccttcgcggt cggccttctg gtcacgctgg cgcacgccaa ggcgcaggtt tcgctcgaga
      241 ccgtcatcgt ctggttcttc atcccggtgg cggactgtat tcgcctgctt atcagccgcc
      301 cgcttcgcgg cgtctcgccg ttcatgggcg atcacgatca tttccaccat cgcctggaag
      361 acaagttggg caagaatcaa ggccttgcca cttatgcggt tgtcatcgcg gcctcttccc
      421 tcattgcgac gctggacccg cgttttgcgc ttgtctgcct ttgcgccctt tcggcgtttt
      481 atttcagttt tgcttggcta agcgatgcga ctacggtatc cgtgtcaaag ccggagtcgg
      541 agtcggacga ggtgactgca actgcgggta acgtcgttcc ccttacgggc gacagcgctg
      601 gcgatcgaaa gcgccagcgg acaacacact gacgagccgc agggaggggc gtccccgcct
      661 gggggcgtgg atgcctctgt tctcgcctta gccgtatgag ggccagtctt ggcgcatgtt
      721 tccgtaaaag acacgctgga attttccttt tcctttctat tccgtaacgc agcgcggatt
      781 gccgcccgca ttgcgctgcc ggcgctcggc ggctggttca cgttctacat cgcgttcttc
      841 ctctacctga cggagcttga aaggtatctc ggcaacccca gtgaccgcgt cgccagcctg
      901 gtgcttggcc tggccaccgc cgggctgttg gtaacgcttt tcatgcacgc gatcatcgtt
      961 tcagccatcg cctcactggc gctcgggctg gaggcgccgg gctggaagta tttcagcatc
     1021 gggcggcggg catggcggct ctatgccgcc gacctgagat gcgttctggt tttcgccgcc
     1081 ttcattgccg cggtacggtt gggcgaagcc gcgataggcc gccttggggt gatgcccggg
     1141 caagtctgga tcgccgatct cgtcatgggc gtcggcctgt actggctgtt cgtccgcttc
     1201 ggtttcctgg tcgccccgat tgccgtggcc aggcgggatg gcgagattct gcggcgcggg
     1261 tggcggcttt cctcgggcca tttctggagc ataaaggcga ttacggccgt cctggcgctt
     1321 cccggctttg ctatcgagtt ggcgggcgag tttcttttgc gtctcttagg tacatttccg
     1381 gagctcggtg cgaccggatc catggcgtcc cttgtggcgc tgtaccgcga aatcctgccc
     1441 gccgttctca tcgcaatcgg agttggctat cttgtcgcgg tcaccctgct gacgctggcc
     1501 agggtgtcgg tctatcgcca gcttatggat cagcccgaga cctagtacgc tccacctcat
     1561 gcatgtcgga caattcgatt tcgatctgcc ggaagaactg atcgcgctca ggccggcccg
     1621 gccgcgcgat gcggcgcgcc tgctggtcgt gcatggcgac gggcgcctcg aacacgccct
     1681 tgtaggcgat ttgggacgct tcctcagggc cggcgacgcg ctggtcttga acgacaccag
     1741 ggtcattcgc
//
LOCUS       DASWPU010000065         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_116668,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000065 DASWPU010000000
VERSION     DASWPU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116668"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VGC27_01375"
     CDS             complement(<1..1315)
                     /locus_tag="VGC27_01375"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEY0281254.1"
                     /translation="MKQEQIIDFLKRNSRLVAVGSAAGIILVSFGAFALLPHGPSQDD
                     TTQQVRYVTAQQDPGSAGAPAPRMVESGMPFSFADLVERVSPAVVSVTATATQAVGVE
                     SNLPDNIPDPFRELFKQFRQGRPPVQRAMSAGSGFIIDKSGLIVTNNHVVDGGKKIKV
                     KLPDGRSFDATLVGADAATDVAVIKIKADKPLPTVEFGNDRQLRVGDWVVAVGNPFGL
                     SNSVTAGIVSSIGRDLGGTNQPYTDFIQIDAPINRGNSGGPTFNLRGQVIGMNSMIFS
                     PTGGSVGIGFAIPSSTVREVVTQLETHGRVARGWLGVQIQSVTPEVAASLGIAEPKGA
                     IIASVVADSPASKAGLRQGDVIVAVNGKPVEDSQGLTRRVASLAAGKKATFTILRDGS
                     NKTVEVAIAQRKDDQVASAAQTPPPSEASATGSAMGLGLAAVTPDL"
     gene            complement(1445..1894)
                     /gene="ccmE"
                     /locus_tag="VGC27_01380"
     CDS             complement(1445..1894)
                     /gene="ccmE"
                     /locus_tag="VGC27_01380"
                     /inference="COORDINATES: protein motif:HMM:NF009731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY0281255.1"
                     /translation="MNAKKRRRLYFALALVAGGAGAAALVVAALQDSVLYFYSPSDVV
                     AKHVAPGVAFRIGGLVEEHSFRKEHGTEIRFTVTDGRAQVPVTYNGVLPALFEEGQGV
                     VAIGRLTPLGTFAANQVLAKHDEKYMPPEVADALKRSGRWQETTGSP"
     gene            2024..2635
                     /locus_tag="VGC27_01385"
     CDS             2024..2635
                     /locus_tag="VGC27_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281256.1"
                     /translation="MKIHRIALAVAAGLVLAGCLPVTTKTPVGTTAGLGADPALIGSW
                     IARSKDQEDKNHKDIGVAYFHFLQIKGAGAINVLWVSASDRKGDNGWMSFSASTAKLG
                     AYNYLNAIWLSENDKPAGDNLKEANFPLLYSFGGDRRLTLYLLDEDKAKEAIKAGKIA
                     GTIEPGNFGDVKITAAPGALDAFFATPEAAKLFKVFIVLRRVE"
     gene            complement(2785..3126)
                     /locus_tag="VGC27_01390"
     CDS             complement(2785..3126)
                     /locus_tag="VGC27_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281257.1"
                     /translation="MPPKSNPLKLNPLQLRTLTLLQGLASLPDSAQREANGEVTITRF
                     PQAHGDHFHLGDAVVAAKDATGLYNEAVWHALARKNLARSDWPHRITLTLDGLAYDTG
                     LGTEIFQRSAH"
     gene            complement(3101..4540)
                     /locus_tag="VGC27_01395"
     CDS             complement(3101..4540)
                     /locus_tag="VGC27_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0281258.1"
                     /translation="MTPQAAFEYLNRIAGIAPLGVPTFEGPEAVIPTPLRAATAAAAS
                     LGLGAAVAGEIWRFRGGDKQSVAVDLKAAAASLVSASLLRRNGEKLPGGGEAVPVTGF
                     YQCADGRWIHLHGGFPHLAMRTLDLLNAENDPKAILDSVSKWSSLALEDALGFMQLCG
                     AVVRSEEEWRAGVQGRVVGPPIVLKKIGEAPPLRLPESATPLDGVRVLDLTRVLAGPA
                     CSRLLATHGADVLTIRAERLPTIAQFDLDTGAGKRLAFLDLAEPADAESLRRLVRGAH
                     VFVESYRPGALSRLGFSPAALAHVAPGMTYVSVSAYGLEGPWAGRRGWEELVQAATGI
                     AVEQGVFVAARRKSRRETFPELIPAAVLDYTTGYLAAAGAMAALLRRIREGGSWLVQV
                     SLAGTAMWLQSLGKIDAASVPESWEPRDGLDPYLVSCDTRSGRLELLGPVARMSKTPP
                     LMGGPPEIADAPRWASAQEGADAAEVQSA"
BASE COUNT          872 a         1571 c         1509 g          817 t
ORIGIN      
        1 gcaagtccgg cgttacggcc gccaatccca gccccatcgc ggaaccggtg gcgctggcct
       61 cgctcggcgg cggcgtctgc gccgcggagg ccacctgatc gtcttttctc tgggcgatgg
      121 cgacctcgac cgttttgttg gagccgtcgc gaaggatggt gaaggtggct ttcttgccgg
      181 cggcaaggct cgccacgcgg cgggtcaggc cttgcgaatc ctcgacgggt ttgccgttca
      241 cggcgacgat cacatcgcct tggcgcaagc ccgctttcga ggcaggactg tcggccacga
      301 cactggcgat gatggcgccc ttcggctcgg caatgccgag cgaagcggct acttccggcg
      361 tcacgctctg gatctgcacg ccgagccagc cgcgcgccac gcggccgtga gtctcgagct
      421 gtgtcacaac ttcgcgcacc gtcgaggagg gaatggcaaa gccgattccg acgctgccgc
      481 ccgtcggcga aaagatcatc gaattcatgc cgataacctg gccgcgcaga ttgaaggtcg
      541 gaccgccgga attgccgcga ttgatcggcg catcgatctg gatgaaatcg gtgtagggct
      601 ggttggtgcc cccaagatcg cgtccgatcg aggagacgat gcccgcggtc acgctgttcg
      661 acagcccgaa cggattgccg accgcgacga cccaatcgcc gacgcgcaac tgacggtcgt
      721 tgccgaattc caccgtcggc agcggcttgt cggccttgat cttgatcacc gcgacgtcgg
      781 tggcggcgtc ggcgccgacc agcgtggcgt cgaagctgcg tccgtcgggc agtttcacct
      841 tgatcttctt gccgccgtcg acgacgtgat tgttggtcac gatcaggcct gacttgtcga
      901 tgatgaagcc ggaacccgcc gacatcgccc tctgcaccgg gggacggccc tgtctgaact
      961 gcttgaacaa ttcgcggaac ggatcgggga tattgtcggg cagattgctt tcgacaccga
     1021 ccgcctgcgt cgccgtggcc gtgacgctga cgacggcggg gctgacgcgc tccaccagat
     1081 cggcgaagct gaacggcatg ccgctctcga ccatgcgcgg cgccggtgcg ccggcgctgc
     1141 cgggatcctg ctgggctgtg acgtaacgca cctgttgggt cgtatcgtcc tgcgacggac
     1201 catgcggcag cagcgcaaag gcgccaaagc tcacgagaat gatgccggca gccgacccta
     1261 cggccaccaa ccggctgttc cgtttaagaa aatctatgat ctgctcctgt ttcatcacgc
     1321 ttttttcccg gttcggctgg aacggtcacc agtccgtcgc ttattccaat acgccctaat
     1381 cattgcttgt ttatatccaa actataaata aaatgtaatg ttatgccgat ggcggcccgg
     1441 aaaatcaggg cgaaccagtg gtttcctgcc agcggccgga acgcttgagc gcatcggcca
     1501 cttccggcgg catgtatttt tcgtcgtgct tggcgagaac ctgattggcc gcgaaggtgc
     1561 ccaagggcgt caggcggccg atcgccacca cgccctggcc ctcctcgaaa agagccggca
     1621 gcacgccgtt atacgtgacc ggtacttgcg cccgcccgtc ggtgacggtg aagcggattt
     1681 cggtgccgtg ctccttacgg aagctatgtt cttcgaccaa cccaccgata cggaatgcca
     1741 cgcccggcgc gacatgcttc gccaccacgt cgctgggact gtagaaataa agcacgctgt
     1801 cctggagcgc cgcgaccacc agagccgccg cgcccgcccc gcccgccaca agcgcgagcg
     1861 cgaaataaag acggcggcgt ttttttgcgt tcatgggttc gcacctgatc cacgagccgg
     1921 actataatgg gccaactccc cgcgtccatc cttcgagacg cccttttgcc tccggcaaaa
     1981 cgggctcctc aggatgaggg ggccttcgat gggggaaaac accatgaaaa tccatcgcat
     2041 cgcattagcc gttgcagcag ggcttgtgct cgcgggttgc ctgccggtga cgaccaagac
     2101 gcccgtcggc acaacggcgg ggcttggtgc cgatccggcg ctcatcggta gctggatcgc
     2161 gcgcagtaaa gaccaagagg acaaaaacca taaagatata ggcgtggcgt atttccattt
     2221 cttgcagatc aaaggcgccg gcgcgatcaa cgtgctttgg gtgtcggcca gcgaccgcaa
     2281 gggcgacaat ggctggatga gctttagcgc cagtaccgcc aagctcggcg catacaatta
     2341 cctgaatgcg atttggctga gcgagaacga caagcccgcc ggtgacaatc tcaaggaggc
     2401 gaattttccc ctgctctaca gcttcggcgg cgaccgcagg ctgacgcttt acctgctcga
     2461 cgaagacaag gcgaaagagg cgatcaaggc cggcaagatc gcgggcacca tcgaaccggg
     2521 caatttcggc gacgtgaaaa tcaccgccgc acctggcgca ctcgacgcct tcttcgcgac
     2581 gccggaagcg gccaagctat tcaaggtttt catcgtgctg agacgggtgg agtagagctg
     2641 ctctatagtt gaagcgtttt tctattttcg tcatggccgg gcttgacccg gccatccagc
     2701 gaaacagaga gttttccgcc gcgcgacgcg cggcaactgg atggccgcct ctggggcggc
     2761 catgacgaaa taaatgaatg ccagctaatg cgccgagcgc tggaagattt cggtccccag
     2821 gccggtgtcg taggccagcc cgtcgagcgt gagcgtgatg cggtgcggcc agtcgctgcg
     2881 cgccaagttc ttgcgggcca gcgcgtgcca caccgcttcg ttgtaaagcc cggtcgcgtc
     2941 cttggcggcc accacggcgt cgccgagatg gaaatgatcg ccatgcgcct gcgggaagcg
     3001 ggtgatggtg acctcgccat tggcctcgcg ctgggcggaa tcgggaaggc tcgccaggcc
     3061 ttgcagcaag gtcagcgtgc gcagttgcag cggattgagc ttaagcggat tggacttcgg
     3121 cggcatcggc accctcttgc gcggatgccc agcggggtgc atccgcaatt tccggcggcc
     3181 cccccatcag aggcggagtc ttcgacatcc gggcgaccgg ccccagaagt tcgagtcgcc
     3241 cgctcctcgt gtcgcacgat accagatacg ggtcgagtcc gtcgcgcggt tcccagcttt
     3301 ccggcaccga cgctgcgtcg atcttgccca gcgattgcaa ccacatcgcc gtgcccgcca
     3361 gcgacacctg caccagccaa ctgccgcctt cgcggatgcg cctgagcagc gccgccatcg
     3421 cgccggccgc tgccagatag ccggtcgtat aatccaagac ggcggcggga atcagctcgg
     3481 ggaaggtttc gcgccggctc ttgcggcgcg ccgctacgaa gaccccctgc tccacggcaa
     3541 tcccggtcgc cgcctggacc agttcctccc agccgcgccg gcccgcccag ggaccttcca
     3601 gcccataggc tgataccgaa acataggtca tgcccggcgc gacatgagcc agcgccgccg
     3661 gcgaaaagcc gagcctggac agcgcccccg gccggtagga ctccacgaag acgtgcgcgc
     3721 cgcgcaccag ccggcgcagc gattccgcat ccgccggctc ggccagatcg aggaacgcca
     3781 agcgcttgcc cgcgccggtg tccagatcga attgcgcgat cgtcggcagg cgctcggcgc
     3841 gaatcgtgag cacgtcggcg ccgtgcgtcg ccagaaggcg cgaacaggcc ggtcccgcca
     3901 gcacccgcgt caagtcgagc acgcgaacgc cgtcgagcgg cgtggcgctc tcgggcaagc
     3961 gaagcggcgg cgcctcgccg atcttcttga gaacgatggg cgggccgacg acccggcctt
     4021 gcaccccggc gcgccattcc tcttcgctgc gcaccaccgc gccgcaaagc tgcatgaagc
     4081 cgagcgcgtc ctccagcgcc aagctgctcc atttggaaac gctgtccagg atcgccttgg
     4141 gatcgttctc ggcgttcaac agatcgagcg tgcgcatcgc cagatgcgga aagccgccat
     4201 gcaggtgaat ccagcgtccg tccgcgcatt gatagaagcc ggtgacggga accgcttcgc
     4261 ccccacccgg cagtttctcg ccgttccggc gcagcagcga agcggacacc agcgacgcgg
     4321 ccgcggcctt caaatcgacg gcgaccgact gcttgtcgcc gccgcgaaag cgccaaatct
     4381 cgccggccac ggcggcgccg aggccgagcg aagctgccgc cgcggtagcc gcgcgcaacg
     4441 gagtcggtat gaccgcctca ggcccctcga aggtgggaac gcccaaaggc gcgatgcccg
     4501 ctatccggtt caggtactcg aaagcagcct ggggcgtcat gcaagtctcc agcgcccccc
     4561 cggtatatcc tcgccgattc aacgccgcaa tgacaggggc ggagggcagg ccatgacaat
     4621 caagttcaag cgagattttg cccttacgcc gccgctgaag cgattgcggc cttggcgcgg
     4681 cggcggagga attcggcttc ctcggcgctg aacagcagcg cggtgagttc gacgacaagc
     4741 tcgaactggc gttcgacgac ggcgcggcg
//
LOCUS       DASWPU010000066         5736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_31069,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000066 DASWPU010000000
VERSION     DASWPU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31069"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1492
                     /locus_tag="VGC27_01400"
     CDS             152..1492
                     /locus_tag="VGC27_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEY0281259.1"
                     /translation="MTASPWHDADRIAAAIRPARFPIRDFLVTAFGATGNGKTDCTAA
                     FASAIGACHRAGGGRVIVPPGIWFTGAIELKSNVNLHLSKGATITFSTDPARYLPQVL
                     TRWEGVELMNYSPLVYAFGQENIAITGEGILDGNADNAHWWPWCGDPRFGWNESILKQ
                     TAARAKLFAMGEANVPVAQRRFGDGSALRPPFVQPYRCRNVLIEGVTIRNAPFWVIHP
                     VLCTNVVVRAVVMDSKGPNNDGCDPESCDHVLIENCTFDTGDDCIAIKSGRNADGRRL
                     GVPSQNIVIRNCLMKNGHGGITVGSEISGGVRQVFVENCRMGGADLGSAIRIKSNAVR
                     GGTLEHIHVRHVTISRVSHAVLTVDLNYEEGANGPFRPVVRDVTLEGVTSGASTYGID
                     LQGLPHSNVSHIVLKNCNLQNVANGNIVQNVHDLTMDNVTINGEIHFTPGLVPH"
     gene            1575..2984
                     /locus_tag="VGC27_01405"
     CDS             1575..2984
                     /locus_tag="VGC27_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEY0281260.1"
                     /translation="MVRLHKLCCAVCSSLLFGSLLAAPAAAQAKVSFASRICNVRDYG
                     ADGARLVPETAAFQKAIDDCTARGGGTVEVPRGWWYIGPIFLKSNINLHVAKYAVVQF
                     ATDPKLYGTTEHPMSAEGKSADLALINITDATNVAITGEGMFDGQGYVWWEWLRNYWR
                     DSEAAHDGEANQRQKVTRPKMLIANNVKNLLLEGATFANSPSFHIVLNRSEDITVRNT
                     SILAPASSPNTDAIDPGNSRNVLIEGNTISCGDDVVAIKGSGVDPAHPDAAVFNIVIR
                     NNKIFAGHGISIGSGTSGGVKHVLIENNSFEGSFHGIRIKTRRGYGGEVSDIVIRNNT
                     MKDVQNVLTIADYFQYAPLDLNIARAQVAKGGMIIVDLMYPPDDDPAQPYVENKTPNI
                     HDVTIDGLTATGAEAAGIVLGIPEKEIANLTMKNVRIEAKTGLIVRNATITTENTTIT
                     AEKGKPVILQRHGRYKTVK"
     gene            complement(3153..4682)
                     /locus_tag="VGC27_01410"
     CDS             complement(3153..4682)
                     /locus_tag="VGC27_01410"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEY0281261.1"
                     /translation="MTKQDPTNNTGVIRLHPADNVVVALNPIPAGTTIARENVTTLDA
                     IPFGHKIAIRPIKTGEPVRKYGQVIGVATKDIAPGAHVHVQNVTTSDLRSEAEKIEVF
                     SSNEMPRSFMGYKRPDGRVGTRNYIAVLTSVNCSATVAKFVAEQATKRGLLKDYPNID
                     GIVPIVHHSGCGMPADGELYDLLVRTLWGTANNPNFVAVLLVGLGCEVPQIARIQKDY
                     GIQTGKKFQSYTIQDVGGTRNAVEEGLKRLALMLPEANQAKRTEQPASELVVGLQCGA
                     SDAWSGVTGNPALGNACDRLVELGGTVILSETPEIYGAEHLLYARAAKPEIAAKLRER
                     IVWWENYTKLNGMEMNNNPSPGNKAGGLTTILEKSLGAVAKAGTSPLNEVVEYAKPIT
                     QHGLVYMDSPGLDPCSATGQVASGANLIAFTTGRGSAYGCKPTPTIKLATNTTMYERM
                     TGDMDINCGTIATGNSSIEEKGAEILDYMLKVASGMKSKSEELGYGDMEFVPWHIGAV
                     M"
     gene            complement(5056..>5736)
                     /locus_tag="VGC27_01415"
     CDS             complement(5056..>5736)
                     /locus_tag="VGC27_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0281262.1"
                     /translation="NCFGQADPNNLYCQQIFRINGTIVGGTGYVNAANANISGLKTQG
                     FDFNGQYSFGLDWGLLTNESMFDVGASWNYLLELVSLPDVTAPGVTSNCAGAFGNTYC
                     FWEPTPRLKGSTRVTWHDGPVSLSLRWRYIDGVTVDKYLIPLRRGTTGLALANFTRPT
                     LPNMNYFDLSASWDINENIELYGGINNLFSKDPPIMGSAASYANSFPATYDAFGRVTF
                     IGVRARTN"
BASE COUNT         1147 a         1757 c         1698 g         1134 t
ORIGIN      
        1 cgtcacaccc cgatttggcc gcatagaggt gttgtccgtc atgcgctccg atccaacgat
       61 aaatactccc ttccggtggc tgtcccgtcg caacatgctg cttgggacca tggcatatgg
      121 cacgttcccg gcgcagtcgt gctgggcgcg agtgaccgcc tccccttggc acgatgccga
      181 cagaattgcc gcggcgatcc gccccgcacg ttttcccatt cgcgatttcc tcgtcacggc
      241 attcggcgca accggcaacg gcaagaccga ttgcaccgct gccttcgcga gcgccatcgg
      301 cgcctgtcat cgggccggcg gcgggcgggt gatcgttccg ccgggcattt ggttcaccgg
      361 tgccatcgag ctgaaaagca acgtcaatct gcacctgtcg aaaggcgcca cgatcacctt
      421 cagcaccgat cctgcccgtt atctgcctca ggtcctgaca cgctgggaag gcgtcgaact
      481 gatgaactat tccccgctcg tctatgcctt cggacaggag aacatcgcga tcacgggcga
      541 aggaatcctc gacggcaacg ccgacaacgc acactggtgg ccgtggtgcg gcgacccgcg
      601 gttcggctgg aacgagagca tacttaagca gaccgccgcc cgcgccaagc ttttcgccat
      661 gggcgaggca aacgtgcctg tcgcccagcg ccgcttcggc gatggaagcg cgctgcgccc
      721 gcctttcgtc cagccctatc gctgcaggaa tgtcctgatc gaaggcgtca ccatccgtaa
      781 tgcgccgttc tgggtgatcc acccagttct ctgcacgaac gttgtggtgc gcgctgtggt
      841 catggacagc aaaggtccca acaacgacgg ctgcgatcct gaatcctgcg atcacgttct
      901 gatcgagaac tgcaccttcg ataccggcga cgactgcatc gccatcaaat cggggcgcaa
      961 tgccgacggg cggcgcctcg gcgtgccgtc gcagaacatc gtgatccgca actgcctgat
     1021 gaagaacggc cacggcggca tcaccgtcgg cagcgaaatc tcgggcggcg tccggcaggt
     1081 tttcgtcgag aactgccgga tggggggggc cgatctcggt tccgccattc gaattaagag
     1141 caatgccgtg cgcggtggca cgctggaaca tatccatgtg cgccatgtca cgatctcgcg
     1201 tgtctcccac gccgtcctca ccgtagatct gaactacgag gaaggcgcga atggtccgtt
     1261 ccggcccgtg gtgcgcgatg tgacgctcga aggtgtgacg agcggggcaa gcacgtacgg
     1321 catcgatctg cagggactgc cgcacagcaa cgtgtcgcac atcgtcctga agaactgcaa
     1381 tctgcagaac gtggccaacg gcaatatcgt gcagaacgtg catgatctga cgatggacaa
     1441 cgtcacaata aacggcgaga tacactttac acccggcttg gtcccgcatt gacatgcctg
     1501 ctggacgttc ctaacgcgtc acccgagtag agccggatga tgctggtccg gcggcgccag
     1561 atgagggaaa tactgtggtt cgacttcaca aactttgttg cgcggtatgt tcgtcgctac
     1621 tgttcggttc gctgctggcc gctccggccg cagcacaggc gaaggtgtca ttcgccagcc
     1681 gcatctgcaa cgtgcgcgat tacggcgccg acggcgcccg gctcgttccc gagaccgcgg
     1741 ccttccagaa agcgatcgac gattgcacag ccaggggcgg cggtaccgtc gaggttccgc
     1801 gcggctggtg gtacatcgga ccgatcttcc tgaagagcaa catcaatctg cacgtcgcca
     1861 aatatgccgt cgtgcagttc gccaccgatc ctaagctata tggcacaacc gagcatccga
     1921 tgtcggcgga aggcaagagc gcggatctcg cccttatcaa catcaccgac gccaccaacg
     1981 tcgccatcac cggcgaaggc atgttcgacg gacagggtta tgtctggtgg gaatggctgc
     2041 gcaactactg gcgcgattcc gaggcggcgc atgacggcga ggccaaccag cgtcaaaagg
     2101 tgacgcgccc caagatgctg atcgccaaca acgtcaagaa tctcctgttg gaaggcgcca
     2161 ccttcgccaa ttcgccgtct ttccacatcg tgctgaatcg ctccgaggac atcacggtcc
     2221 gcaatacctc gatcctggca ccggcgtcct cgcccaacac cgatgcaatc gatccgggca
     2281 acagccgcaa cgtgttgatc gagggcaaca ccatcagctg cggcgacgat gtggtggcca
     2341 tcaaaggctc cggcgtcgat cccgcccatc ccgatgccgc agttttcaac atcgtgatcc
     2401 gcaacaacaa gattttcgcc ggccacggca tttccatcgg cagcggcact tcgggcggcg
     2461 tcaagcacgt gctgatcgag aacaacagct tcgagggctc gttccacggc atccgcatca
     2521 agacacggcg cggctacggt ggcgaggtgt cggacatcgt gatccgcaac aacacgatga
     2581 aagacgtcca gaacgtgctg accatcgccg actatttcca atatgcgccg cttgatctca
     2641 atatcgcgcg cgcccaggtc gccaagggcg gcatgattat cgtcgatctg atgtatccgc
     2701 cggacgacga cccggcccag ccttacgtcg agaacaaaac gccgaacatc cacgacgtga
     2761 ccatcgacgg cctcaccgcc acaggcgcgg aagcggccgg catcgtgctc ggcattccgg
     2821 aaaaggaaat cgccaatctg acgatgaaga acgttcgtat cgaggccaag actggcttga
     2881 tcgtacgcaa cgcgacgatc actaccgaaa acaccacgat cacggcggaa aaaggcaagc
     2941 ccgtcattct gcagcgccac ggccggtaca aaactgtaaa gtgatctgat acacccccgc
     3001 aagaatcacc ctgccggacc gtccggtagg gcttttttgc catctcagtc tgtagggcgc
     3061 ggcgacattc cggccggcag cacaatggca gattcaaaag cggacgttcg acggttcgag
     3121 ggatttccgt tgttccgccg gataccgggc agctacatca cggcgccgat gtgccagggc
     3181 acgaattcca tgtcgccgta gccaagctct tcactcttgc tcttcatgcc ggacgcgact
     3241 ttgagcatgt agtcgaggat ttccgcgccc ttttcttcga tgctggagtt gccggtggca
     3301 atcgtgccgc aattgatgtc catgtcgccg gtcatacgct catacatggt ggtgttggtc
     3361 gcgagcttga ttgtcggcgt gggtttgcag ccgtaagccg agccgcgccc ggtggtaaag
     3421 gcgatcagat tggcgcccga cgccacttgg ccggtggccg agcagggatc gaggccgggg
     3481 ctatccatgt aaaccagccc gtgctgggtg atcggcttgg cgtactcgac gacctcgttg
     3541 agcggcgaag tacccgcctt ggccaccgca cccaaggatt tctccagaat ggtggtcagc
     3601 ccgcccgctt tgttgccggg cgaagggttg ttgttcatct ccatgccgtt cagcttggtg
     3661 tagttctccc accagacgat gcgctcgcgc aatttcgcgg cgatttccgg cttggcggcg
     3721 cgagcataca agagatgctc ggcgccgtag atttcggggg tctccgacaa gatcacggtg
     3781 ccgcccaatt ccaccagccg gtcgcaggca ttgcccagcg ccgggttgcc ggtgacgccc
     3841 gaccaggcat cggaagcgcc gcattgcagg ccgacgacca gctcgctggc cggctgctcg
     3901 gtgcgtttgg cctgattggc ttcgggtagc atcagcgcca gccgtttcag gccctcctcg
     3961 acggcattgc gcgtgccgcc gacatcctga atcgtatagg actgaaactt ctttccggtc
     4021 tggatgccat agtccttttg gattcgggcg atttgcggca cctcgcagcc caaacccacc
     4081 agcagcaccg ccacgaagtt cgggttgttc gccgtgcccc acaaggtacg gaccaaaagg
     4141 tcgtataatt cgccgtctgc cggcatgccg cagccgctgt gatgaacaat cggcacgata
     4201 ccgtcgatgt tgggataatc cttcaacagg ccgcgtttgg ttgcttgttc ggcgacgaac
     4261 ttggctacgg tagccgaaca gttcacggag gtgagcacgg cgatgtaatt gcgggtgccc
     4321 acccgtccat cggggcgctt gtagcccatg aagctgcggg gcatttcatt ggagctgaaa
     4381 acttcgatct tctctgcttc cgagcgcagg tcggaagtgg tgacgttctg gacatggaca
     4441 tgtgcgccag gggcaatgtc cttggtggcg acaccgatca cctggccgta tttgcgcacc
     4501 ggctcgccgg tcttgatcgg gcggatggcg atcttgtggc cgaaaggaat ggcgtcgagc
     4561 gtggtgacgt tctcacgggc gatcgtggtg ccggcgggaa tgggattcaa cgcgacaacc
     4621 acattgtcgg ccgggtgcag acgaatgaca ccggtgttat tcgttgggtc ctgttttgtc
     4681 acgatctaac cttcaatgtc ttgaactggt gaactcttct attcataagc cttctgcgac
     4741 ccggtgtggg gtttcgccag gctctgcgac gccggcagtg tcgaagtgac gaggccggca
     4801 atctccgcag ccagaacaaa gtcacgacga ttgaagcgac gagaaggcat caaatcctcc
     4861 gtccggccgt tgccgtcagc actcctatcc cggccgttat gccgcccacc tcaacgcctt
     4921 gaaaaaggtg cgccccagag gaggtttctg gggcgcgctc acgctagaaa gaaaggcacg
     4981 acggaaagac attcaattga aaatatttcg tcttccaaaa atgcatcgtg ccttttctca
     5041 tccttacact tcagatcagt tcgtcctggc cctgacaccg atgaacgtca cgcgaccgaa
     5101 cgcgtcatac gtcgccggga aggagttcgc ataactcgcg gcacttccca tgatcggcgg
     5161 atctttgctg aacaggttgt tgatgccgcc ataaagctcg atgttttcgt tgatgtccca
     5221 cgaggccgaa aggtcgaagt agttcatgtt cggcagagtc ggccgcgtga agttggccag
     5281 tgccagacct gtggtgccgc gacggagcgg gatgaggtac ttatctaccg tcacgccatc
     5341 gatataccgc caacgcaggc tcagagaaac cggcccgtca tgccatgtga cgcgcgtcga
     5401 acccttgagc cgtggcgtcg gctcccagaa gcagtaggtg ttgccgaacg cgccggcgca
     5461 gttgctcgtg acacccggtg cggtcacgtc gggcagcgag accaattcca acaggtagtt
     5521 ccagctcgcg ccgacgtcga acatgctctc gttggtgagc aaaccccagt cgagtccgaa
     5581 actgtactgc ccgttaaaat cgaagccctg agtcttcagt ccgctgatgt tggcgttcgc
     5641 ggcgttgacg tagccggtgc cgccgacgat cgtgccgttg atacgaaaga tctgctggca
     5701 atagaggttg ttcggatcgg cttggccgaa gcagtt
//
LOCUS       DASWPU010000067         5320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_37313,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000067 DASWPU010000000
VERSION     DASWPU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37313"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VGC27_01420"
     CDS             complement(<1..1154)
                     /locus_tag="VGC27_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY0281263.1"
                     /translation="MAHNNGMVPRGLRGSAASPSQEGGFAETARGFGDMTGAEIAGFV
                     PHRPARPEKSEGGKRFVLVSEYEPAGDQPTAIAELVRGANSGERDQVLLGVTGSGKTF
                     TMAKVIEIVQRPALILAPNKTLAAQLYAEMKNFFPENAVEYFVSYYDYYQPEAYIPRT
                     DTYIEKDSSINEEIDRMRHSATRAILERDDVIIVASVSCIYGIGAVETYSGTAVTIKQ
                     GARTDRTELMRQLSALQYRRNDDNFVRGAFRVRGDTIDIFPAHYEDRAWRIQLFGDDV
                     DSIREFDPLTGRSAGTLETIKIYANSHYVTPRPTLGQALKGIKEELRLRLDQFRAEGK
                     LLEAQRLEQRTNFDMEMIEATGSCAGIENYSRWLTGRKPGEPPPTLFEYIP"
     gene            1497..2630
                     /locus_tag="VGC27_01425"
     CDS             1497..2630
                     /locus_tag="VGC27_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281264.1"
                     /translation="MRVIGVKHDGLGKFRRIAGYGVVEQKAAAQKQLWDKQWQRRREL
                     ERKLPADSTVLQALKKIQAEEQSEEARREILSLTSILLDALRARPAVDWAQFYDGGEF
                     SEPPPAEPASPRTPEAPREAEFKPAPPRSLFELLHARRRRELKAASERKFKNAYDDWQ
                     HSVRWKTRQYEAAAERYRAALAGWEARKSAFYAAQAEAVARLNSLHQRYAGKDADAVI
                     AYSDMVLLSAGRPDGFPKHWTIAFAGGMMTVDYELPSTEAVPGVKAVKYLPSRDAFET
                     VELPPRERDQLYAEAMYQTCLSVLHLLFAADEAGAIKSIIFNGWANFIDKARPARACI
                     MTVQTAKTDFSRIDLASVDPRACFKSLNGVASAKLAAMTEAAP"
     gene            complement(2713..3018)
                     /locus_tag="VGC27_01430"
     CDS             complement(2713..3018)
                     /locus_tag="VGC27_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY0281265.1"
                     /translation="MALKRALPPTHPGEILREDVLPALGLSVMDAAAKLGVTRQTLHR
                     IIAKKNPRPVTPEMAVRLGKLCGNGPRLWLNLQSAYDLWHAERRIDTRKIPTLEAAE"
     gene            complement(3008..3292)
                     /locus_tag="VGC27_01435"
     CDS             complement(3008..3292)
                     /locus_tag="VGC27_01435"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0281266.1"
                     /translation="MIGSFRSKALRNLWEKDDPTGVGASVRARIGRILSILESAESIG
                     DLDVPGYRFHALKGFVPRRYSMRVTGNRRITFAWHDGTANAVDLEDYHGS"
     gene            complement(3352..3669)
                     /locus_tag="VGC27_01440"
     CDS             complement(3352..3669)
                     /locus_tag="VGC27_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY0281267.1"
                     /translation="MSKARRTARSTQLPLVTPGEILAEEFLGPMGLSQNALARAIRVP
                     PRRINEIVLGVRAVTADTDLRLARYFGMSEGFFLGLQTDYDLRAKKREIGGDLALIRP
                     KAA"
     gene            complement(3662..3949)
                     /locus_tag="VGC27_01445"
     CDS             complement(3662..3949)
                     /locus_tag="VGC27_01445"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0281268.1"
                     /translation="MIRSFRDAETKIVWEGRRSRKLLFDIQATALRKLMMLHRSRTLN
                     DLRVPPANRPEPLKGSRAGQWSIRTNRQWRICFRWNEGDANDVEIVDYHDE"
     gene            complement(4097..4282)
                     /locus_tag="VGC27_01450"
     CDS             complement(4097..4282)
                     /locus_tag="VGC27_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281269.1"
                     /translation="MPSYEIHYMNDDGTLDAKVAADCANDRQAKVLAHALKSKGLRRI
                     KVWEGDNLVYERPHRLQ"
     gene            4501..4851
                     /locus_tag="VGC27_01455"
     CDS             4501..4851
                     /locus_tag="VGC27_01455"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEY0281270.1"
                     /translation="MADVRGPVASADSARTMTIIIYLLYLASLVNGITAIIGVALAYA
                     KREDARGTPYDSHFSNAIEIFWIFLAGMLVAVPLCFVLIGIPLVIALYVWVIFRTVKG
                     LVRAVDGRPYFYTG"
     gene            complement(4886..>5320)
                     /locus_tag="VGC27_01460"
     CDS             complement(4886..>5320)
                     /locus_tag="VGC27_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281271.1"
                     /translation="MMLGGGLRDTLLLAWGISTNAFLLIAVSIAATLAAFAAHRPLTG
                     TLSLLLLPILSLVLPMIAVLTARHGDCAINSDGIGACTLWGAKMGMSFHTAASVPDII
                     YGLMPFSFSLTLMLGLLGFFFARPRERKAPHLSEQMRHFEEK"
BASE COUNT          935 a         1661 c         1717 g         1007 t
ORIGIN      
        1 gggatgtatt cgaacagggt cggcggcggc tcgcccggct tgcggcccgt cagccagcgc
       61 gaataattct cgatgccggc gcaggagccg gtggcctcga tcatctccat gtcgaaattg
      121 gtgcgctgct ccaggcgctg cgcttccagc agcttgccct cggcgcggaa ctggtcgaga
      181 cgcaggcgca gctcttcctt gatgcctttc agcgcctggc ccagcgtcgg gcgcggcgtg
      241 acgtaatgcg aattggcgta gattttgatc gtctccagcg tgcccgccga gcggccggtg
      301 agcggatcga attcgcggat cgagtccacg tcgtcgccga aaagctggat gcgccaggcg
      361 cggtcttcgt aatgggccgg gaaaatgtcg atggtgtcgc cgcgcacgcg aaaggcgccg
      421 cgcacgaaat tatcgtcgtt gcgccggtat tgcagggccg aaagctggcg catcagttcc
      481 gtgcggtcgg tgcgcgcgcc ctgcttgatc gtcaccgccg tgcccgaata ggtttccacc
      541 gccccgatgc cgtagatgca cgacaccgac gccacgatga tcacgtcgtc gcgctccagg
      601 atcgcccgcg tcgccgaatg gcgcatgcgg tcgatctcct cgttgatcga agaatccttt
      661 tcgatatagg tgtcggtgcg cgggatatag gcttcgggct ggtagtagtc gtaataggag
      721 acgaaatact ccaccgcgtt ctcgggaaag aagttcttca tctcggcgta aagctgggcg
      781 gccagcgtct tgttcggcgc caggatcagc gccgggcgct gcacgatctc gatcaccttg
      841 gccatggtga aggtcttgcc cgagccggtg acgccgagca gcacctggtc gcgctcgccc
      901 gaattggcgc cgcgcaccag ctccgcgatg gcggtgggct ggtcgcccgc cggctcatac
      961 tcgctgacca gcacgaagcg cttgccgcct tcggatttct ccggccgcgc cgggcggtgg
     1021 ggcacgaaac cggcgatctc cgcgccggtc atgtcgccga agccgcgggc ggtctccgcg
     1081 aatccgcctt cctgggaggg cgaagcggcg gagccgcgaa gacccctggg aaccattccg
     1141 ttgttgtgcg ccatgggggc aatataggcg ttgttcgcgc tttgtgcagc ccagccccgg
     1201 aggccattat tccggccgcg cttgaggcca ggcatccgtc ccgaacacgg gggtgaaaac
     1261 gcgatgacgg ttcgctaagc cggaggagaa ggagaggcgc gtgcggcaaa cgaaatatcc
     1321 tgccgccgtg ttttccgcgc gtcataaaat tccgctcaaa ttctcttcgc ggcgttggtc
     1381 gcgcgcagga atgccgatct tctgcgcaat tgcacaaata tagagcacgg tccgaaattg
     1441 tgcgttttgc ggatttgtgt tttcctaagc ggcaaaagag tggggggctt tcagatatgc
     1501 gtgtgatcgg cgtcaagcat gacggcttgg ggaaattccg ccgcatcgcc ggctatggcg
     1561 tcgtcgagca aaaggccgcc gcccagaagc agctttggga caagcaatgg caaaggcggc
     1621 gggagctgga gcgcaagctt cctgccgatt ccaccgtcct tcaggcgctg aaaaaaatcc
     1681 aagccgagga gcagagcgag gaagcaaggc gcgagattct ttcgctgacc tcgatcctgc
     1741 tcgacgcgct gcgtgcgcgc cctgcagtcg attgggcgca gttctacgac ggcggcgaat
     1801 tctccgaacc gccgcccgcg gagccggcct cgccgcgcac gcccgaggca ccgcgcgaag
     1861 cggaattcaa gcccgcgccg ccgcgctctc tgttcgagct gctgcatgcg cgccgccgcc
     1921 gcgaactcaa agcggcgagc gaaaggaagt tcaagaacgc ctatgacgat tggcaacact
     1981 cggtgcgctg gaaaacgcgc cagtacgagg cggcggcgga acgctaccgc gccgcgctgg
     2041 ccggatggga ggcgcgcaag tcggcttttt atgccgccca ggccgaggcc gtggcgcggc
     2101 tcaattccct gcaccagcgt tacgccggca aggatgcgga cgccgtcatc gcctatagcg
     2161 acatggttct gctctcggcc ggccggcccg acggttttcc gaaacattgg acgatcgcgt
     2221 tcgcaggcgg catgatgacg gtcgattacg aactgccctc caccgaggcg gtgccggggg
     2281 tgaaggcggt gaaatacctc ccctcgcgcg acgccttcga gaccgtcgaa ttgccgccgc
     2341 gcgaacggga ccagctttac gccgaagcga tgtaccagac ctgtctttcg gtcctgcatc
     2401 tgttgttcgc cgcggatgag gcgggcgcga tcaagtcgat catcttcaac ggctgggcga
     2461 acttcatcga caaggcgcgg cccgcgcgcg cctgcatcat gacggtgcag acggcaaaga
     2521 cggatttcag ccggatcgat ctggccagcg tcgatccgcg ggcctgcttc aaatccctga
     2581 acggcgtcgc cagcgccaaa ctggcggcga tgacggaagc ggcgccgtaa ggcggacggc
     2641 acggcgggcg gaccttgcgc aaaatgctgc ggacagcggt gggggttagg ggacgttcgg
     2701 cagtttgcgc catcactccg ccgcttcaag cgtcggaatc ttccgcgtgt cgatgcggcg
     2761 ctcggcgtgc cacagatcgt aggccgattg cagattgagc cacagccggg ggccgttgcc
     2821 gcacagcttg ccgagccgca ccgccatctc cggcgtcacc gggcgcggat tcttcttggc
     2881 gatgatgcgg tgaagggtct gacgcgtcac gccgagcttc gcggcggcgt ccatgaccga
     2941 aaggccgagc gcgggcagaa cgtcctcgcg caggatttct cccggatggg tcggcggcag
     3001 cgcgcgctta agagccatga taatcctcca gatcgacggc attcgccgtg ccgtcatgcc
     3061 aagcaaaagt gatccgccgg tttcctgtca cgcgcatgga atagcgcctc ggtacaaatc
     3121 ctttaagcgc gtgaaagcga tatcccggta cgtcgaggtc gccgatactt tccgcacttt
     3181 ccaggatcga caggatgcgc ccgatccgtg cccgcacgct cgcgccgacg ccggtgggat
     3241 cgtccttctc ccacagattg cggagcgcct tgctgcggaa cgatccgatc atgtgtaact
     3301 tataaggtta cagtgcgccc atgtcaagcg gcgagttaca ctgcgcaaag cttatgccgc
     3361 tttcggccgg atcaacgcca gatcgccacc gatctcccgc ttcttggctc tgaggtcgta
     3421 atcggtttgc aggccgagga agaagccctc cgacatgccg aaatagcgcg ccagcctgag
     3481 gtcggtgtcg gcggtcaccg cgcgcactcc cagcacgatc tcgttgatgc ggcgcggcgg
     3541 cacgcggatg gcgcgcgcca aagcgttctg gctgaggccc atcggcccca ggaactcctc
     3601 cgccaggatt tcgcccggcg tgacgagcgg aagctgtgtg ctccgcgccg tcctgcgcgc
     3661 cttactcatc gtggtagtcg acgatttcga catcgttagc gtctccttcg ttccagcgga
     3721 agcaaatgcg ccattgccgg ttggtccgga tgctccactg gccggcgcgg ctgcctttca
     3781 aaggctccgg ccggttggcg ggcggcacgc gaagatcgtt cagcgtgcgc gaccggtgca
     3841 gcatcatcag cttgcgcaac gcagtcgcct ggatatcgaa gagcagcttg cggcttctgc
     3901 gtccctccca gacaattttc gtctccgcgt cgcggaagct ccgaatcatg cccggctata
     3961 acgcataacg ttatgcaacg caacgcgccg atggatcgtc cggcaaggcc cgttcgctat
     4021 tccgtaccct tcagcctctc cgccgtcttg gcggcgtgaa gcggtgcctt ggcgatgtcg
     4081 ggcgaccggt catgcgctat tgaagccggt gcgggcgttc gtaaacgaga ttgtcgccct
     4141 cccacacctt gatgcgcctc agccccttgc tcttcaaggc gtgcgccagc actttggctt
     4201 gccggtcatt ggcgcagtcg gccgcgactt tcgcatccag cgttccgtcg tcgttcatgt
     4261 agtggatttc gtagctcggc atgcgtcgtc tccttcgact attccatgcc ccctgctggc
     4321 ctaaaccttg tgccacgcgc ctttgaatgt cgaatgacaa atccttagga atgctcataa
     4381 atttacgtgg cagatcggcg aaggatggtg cgttgcggca agtcgcggcc gggacgtttt
     4441 gttgacattt ccttgcgggc tacccacatc attcgggaac agtcctggga gggatgagcc
     4501 atggcggacg tccgcgggcc cgttgcgagc gccgattccg cgcgcacgat gacgatcatc
     4561 atctatctgt tgtatctcgc atcgctggtc aacggcatca ccgccatcat cggcgtggcg
     4621 ctggcctatg ccaagcgcga ggacgcgcgc ggcacgccct acgacagcca cttctccaac
     4681 gcgatcgaga tattctggat tttcctggcc ggcatgttgg tggcggtgcc gctgtgtttc
     4741 gtgctgatcg gcatcccgct ggtgatcgcg ctttatgtct gggtgatttt ccgcaccgtg
     4801 aagggcctgg tgcgcgccgt cgacggcagg ccgtatttct acacgggctg acgcttgacg
     4861 atgaaggcgg tcggcatcgc cgccgctact tttcttcaaa gtgccgcatt tgttcgctga
     4921 ggtgcggagc cttgcgctcg cgcggccgcg cgaagaagaa gcccagcaga cccagcatca
     4981 gggtcaagga gaacgagaac ggcatcaggc cgtagatgat gtcggggacg ctggcggcgg
     5041 tatgaaaact catgcccatc ttcgcgcccc acagcgtgca agcgccgatc ccgtcgctgt
     5101 tgatggcgca atcgccgtgg cgggcggtga gcaccgcgat catcggcagc accagcgaca
     5161 ggatcggcag cagcagcaga ctcagcgtac cggtcagcgg gcgatgcgcg gcgaaggcgg
     5221 ccagcgtggc ggcgatcgaa acggcgatca ggagaaacgc gttggtggag attccccagg
     5281 cgagcagcaa ggtgtcgcgc agaccgccgc ccagcatcat
//
LOCUS       DASWPU010000068         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_98328,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000068 DASWPU010000000
VERSION     DASWPU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98328"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..882)
                     /locus_tag="VGC27_01465"
     CDS             complement(211..882)
                     /locus_tag="VGC27_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY0281272.1"
                     /translation="MAYTLILGTKNWSSWSLRPYLAMRATGAPFEEIGIRLRHSESPT
                     TREQILKHSPAGRVPVLKIEENGRTTTVWDSLAICETLAERHPEAKLWPDDAGRRALA
                     RSYAAEMHAGFPDLREQLTMEFARTLPSPGLSDASKAQIARVIEAWTLALNEHGGGGF
                     LFGRFSIADCMYAPVVSRFLTYSIALPPSVAAYCERMMALPAMQDWGRAAKAEVDAGL
                     AFYYA"
     gene            953..2335
                     /locus_tag="VGC27_01470"
     CDS             953..2335
                     /locus_tag="VGC27_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEY0281273.1"
                     /translation="MHPSLVRSAKGAVALHAVAHSDLKRFLAACSKREAAFLKASGFA
                     ANEDELKLVPDAAGGLAFAVLGLGKGGDPLAPALFSEKLPAGVYRYAMPPFSSALATL
                     AWALGAYRFARYRNKDEPSPKLVVPEGVDGAEVGRIAEGVFLARDLINTPPNDMGPQE
                     LSDAARALAKRHKARFSEIVGEALKRKNYPLIHAVGQGSARPPRLIDLVWGRPGAPKV
                     TLVGKGVCFDTGGYDLKTASGMAAMKKDMGGAAVALAVASMAMSARLDLRLRVLIPAA
                     ENSVSGGAYRPSDVFPSRKGLTVEIGNTDAEGRLVLADALAEADSEAPDLLIDVATLT
                     GAARIATGFELPPFFTDDEMLAAELMTYAAEVHDPLWRLPLWRGYEPALASPVADLTN
                     APDYPYAGAITAALFLNRFVAQAKSWVHFDIAAWTDRPRPGRRRGAEATAARAIYALL
                     KERYGKASSK"
     gene            complement(2440..>3390)
                     /locus_tag="VGC27_01475"
     CDS             complement(2440..>3390)
                     /locus_tag="VGC27_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEY0281274.1"
                     /translation="ERSAMAKPNLLMIVPAKWTDLETAAPGECAVNILLHGRDAYAPE
                     NIDYVLSFRPPQGLLKSFSNLKAAFALGAGVDGFLADPAYPKRVPLVRFADRTLSAEM
                     AQFVLMHVLIRHRQQRLFDEAQAKRQWQQMMLPRRTEDTRIGMLGLGEIGSFTAARLI
                     EFGFPVASWSRSRKNLPGLKSFAGEAELDAFLARTDILVCLLSLTRKTAGILNAKAFA
                     AMPQDGFVINVARGEHLVEADLIAALDSGHLSGAVLDVFDQEPLPETSRLWRHPKITV
                     TPHIAAISQPAIVFGTVLGGIAAFERGEKPDHIVDVEKAY"
BASE COUNT          572 a         1100 c         1120 g          598 t
ORIGIN      
        1 tgatcgtcca caccggcaat aatacgggct tggagccttg agccggttct ggcgcattcc
       61 ggccgttgga ctggattttt aaccgcttgg cgtcgagagc cgccgtagcg cagcgaaggc
      121 gggcaaactc agtatgaagg acaaggtact cccctcaccc tgagcctgtc gaagggtgag
      181 gggagggatt cagctggggc ggatcacgtt ctaggcgtag taaaacgcca gcccggcgtc
      241 cacttcggct ttcgccgcgc ggccccaatc ctgcatcgcc ggcagcgcca tcatccgttc
      301 gcaataggct gcaacggaag gcggcagcgc gatggaatag gtgagaaagc gcgaaaccac
      361 cggcgcgtac atgcaatcgg cgatggagaa gcgtccgaac aggaatccgc cgccgccatg
      421 ctcgttcaag gccagcgtcc aggcttcgat gacgcgcgcg atctgcgcct tgctggcgtc
      481 gctcaggccg ggcgagggca gggtgcgggc gaattccatc gtcagttgct cgcgcagatc
      541 gggaaagccc gcatgcatct cggcggcata ggagcgcgcc aacgcgcgcc gcccggcatc
      601 gtcgggccac agcttcgcct cgggatggcg ctcggccagc gtctcgcaga tggcgaggct
      661 gtcccacacc gtcgtcgtgc gcccgttctc ttcgatcttg agcaccggca ccctgcccgc
      721 cggggagtgt ttcaggattt gctcgcgcgt cgtcggcgac tcgctgtggc gcaggcggat
      781 gccgatttcc tcgaacggcg cgccggtcgc gcgcatcgcc agatagggcc gcaggctcca
      841 gctcgaccag ttcttggtgc cgaggatcaa agtatacgcc atggcgtttc tccggttgcg
      901 ggcgggacgc gctgtttcta aggtctcggc cgacagactc aaaggccgtt ccatgcatcc
      961 cagccttgta aggagcgcga agggcgcggt ggcgctgcat gccgtcgcgc acagcgatct
     1021 caagcgcttt ctggccgcgt gctccaagcg cgaagccgcg ttcttgaagg cgtcgggctt
     1081 cgctgcaaac gaagacgagc tgaagctggt gccggatgcg gcgggaggtt tggccttcgc
     1141 cgttctgggg ctgggcaagg gcggcgatcc cttggccccg gctctgttca gcgaaaagct
     1201 tccggcgggc gtctatcgct atgccatgcc gcccttttcg tccgcgctgg cgacgctggc
     1261 ctgggcgctc ggcgcctatc gcttcgcgcg ctatcgcaac aaggacgagc cgtcgcccaa
     1321 actggtggtg ccggagggcg tggacggcgc cgaggtcggc cgcatcgccg aaggcgtttt
     1381 cctggcgcgc gatctgatca acactccgcc caacgacatg gggccgcaag agctttccga
     1441 cgcggcgcgc gcgctcgcca aacggcataa ggcgcgcttc tccgaaatcg ttggcgaggc
     1501 gctcaagcgg aagaattatc cgctgatcca tgcggtgggc caaggctccg cgcgcccgcc
     1561 gcgcctgatc gatctcgtct ggggccggcc gggcgcgccc aaagtcacgc tggtgggcaa
     1621 gggcgtgtgc ttcgataccg gcggctacga tctgaaaacc gcctccggca tggcggcgat
     1681 gaagaaggac atgggcggcg ccgcggtcgc gctcgccgtg gcctctatgg cgatgagcgc
     1741 caggctcgac ctccgcctgc gggtgctgat tccggcggcg gagaattcgg tgtcgggcgg
     1801 cgcctatcgt ccgtccgacg tgttccccag ccgcaagggg ttgacggtgg agatcggcaa
     1861 taccgacgcc gaaggccgtc tggtgttggc cgacgcgctc gccgaggccg actcggaagc
     1921 acccgatctg ctgatcgacg tcgccacctt gaccggcgcg gcgcgcatcg ccaccggctt
     1981 cgaactgccg cccttcttca ccgacgacga aatgctggcc gccgaattga tgacctatgc
     2041 ggcggaagtc cacgatccgc tctggcggct gcccttatgg cgcggctacg aaccggcgct
     2101 ggcaagcccc gtcgccgatc tcaccaacgc gcccgattat ccctatgccg gagcgatcac
     2161 ggcggcgttg tttctcaacc gcttcgtcgc ccaggcgaag tcctgggtgc atttcgacat
     2221 cgccgcttgg accgaccgtc cgcgtccggg ccggcgccgc ggcgccgaag ccactgccgc
     2281 gcgggcgatc tatgcgctgc tgaaggaaag atacggaaag gcgagtagca aatagggttt
     2341 tttgccccat gcgtttctgc ccccgctata gcggggcaag tgccgacccc ggccttgagc
     2401 cgggggaggc gaagggggtt ctattcgcta ttcaccaatt taatacgcct tctccacatc
     2461 cacgatatgg tccggctttt cgccgcgttc gaacgcggcg atgccgccca gcacagtgcc
     2521 gaagacgatg gcgggctggg aaatcgccgc gatgtgcggt gtcaccgtga tcttgggatg
     2581 gcgccacagc ctgcttgttt ccggcaacgg ctcttggtcg aagacgtcga gcacagcgcc
     2641 ggaaagatgg ccgctgtcca gcgccgcgat cagatcggct tccacaagat gttcgccgcg
     2701 cgccacattg atgacgaagc cgtcttgcgg catcgccgcg aaagccttcg cgttgaggat
     2761 tccggcggtc ttgcgcgtca gcgacagcag gcagaccaaa atgtccgtcc gggcgaggaa
     2821 ggcatcaagc tccgcctcgc ccgcgaagct cttcagcccg ggaagattct tcctgctccg
     2881 gctccagctc gccaccggaa aaccaaactc gatcagccgc gcggcggtga aactgccgat
     2941 ctcgcccagc cccaacatgc cgatgcgcgt gtcctcggtg cggcgcggca gcatcatctg
     3001 ctgccattgg cgtttggcct gagcctcgtc gaacaggcgc tgttgccggt gacggatcag
     3061 cacatgcatc agaacgaact gtgccatctc cgccgaaagc gtcctgtcgg cgaagcgcac
     3121 cagcggcacg cgcttgggat aagcgggatc ggcgaggaag ccgtccacgc ctgcgcccag
     3181 cgcgaaagcg gccttcaggt tcgaaaaact tttcagcaat ccctggggcg ggcggaagct
     3241 cagcacatag tcgatgtttt cgggcgcata agcgtcgcgg ccgtggagca ggatgttgac
     3301 ggcgcattcg cccggcgcgg cggtttccag gtccgtccac ttcgccggca cgatcatcag
     3361 caggttcggt ttggccatgg cggaacgctc
//
LOCUS       DASWPU010000069         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_6661, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000069 DASWPU010000000
VERSION     DASWPU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6661"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VGC27_01480"
     CDS             <1..285
                     /locus_tag="VGC27_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinol
                     3-O-methyltransferase/2-polyprenyl-6-hydroxyphenol
                     methylase"
                     /protein_id="HEY0281275.1"
                     /translation="VKPGGLMFVASLNKTLKSLVLAKIAAEYVLRWLPPGTHDWNRFV
                     EPHTLQAMLEEAGLNILKTQGVSFDPLGWNWRLSTDLDVNYMVVSASPRR"
     gene            complement(391..810)
                     /locus_tag="VGC27_01485"
     CDS             complement(391..810)
                     /locus_tag="VGC27_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009374 - biotin binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="avidin/streptavidin family protein"
                     /protein_id="HEY0281276.1"
                     /translation="MHAGKVSIAIVFALLVGGLSVPSQAQTLAAGTSWQNGLGSTLTI
                     TNVQATGQFSGYYVTAVASPGCQAVGIQQPLIGWISQSNAITFAVNWTAAGCNSVTAW
                     SGNYNPNNGNISTIWILSSSPGWSNMNIGSDTFVPRP"
     gene            complement(911..1327)
                     /locus_tag="VGC27_01490"
     CDS             complement(911..1327)
                     /locus_tag="VGC27_01490"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY0281277.1"
                     /translation="MIVYNLRCGSGHEFEGWFQSSAAFDDQAVTGKLICPICTSRKVE
                     KAIMAPAVAGTKKSVSAKDELKKMRQFVSGMRKYVKENADYVGPRFPEEARKIHYGET
                     EERHIYGEATLKEAKELIEEGVDVAPLPPDLDETAN"
     gene            complement(1393..1857)
                     /locus_tag="VGC27_01495"
     CDS             complement(1393..1857)
                     /locus_tag="VGC27_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0281278.1"
                     /translation="MILRIESPGSAAAQSLIAALDEDIVRRYPGEPTNGIDVAEFEAA
                     GGVFVVCYVNDMPVACGAFRPFEGSAEIKRMYVLPALRGRGLARAVLDFLEAEAFRRG
                     FSRAILETGRQMTEALGLYRSRGWTEIPVFGPYVGDPKSICFEKRLAKPLQS"
     gene            complement(1854..>2380)
                     /locus_tag="VGC27_01500"
     CDS             complement(1854..>2380)
                     /locus_tag="VGC27_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY0281279.1"
                     /translation="SFLIDPKGAIAARYDKIHLFDVDLPSGEKYRESKTVAGGGRAVT
                     ADLPWGRIGLSVCYDLRFPRLYRMLAQAGAFLLAIPSAFTETTGKAHWHVLLRARAIE
                     NGAFVLAPAQGGTHARGRKTYGHSLIVAPWGEVLAEAGTDPGVIVADIDPALAADARA
                     RIPNLKHEKPFEVS"
BASE COUNT          479 a          696 c          709 g          496 t
ORIGIN      
        1 gtgaagccag gcgggctgat gttcgtggcc agcctcaaca agacgctgaa gtccctcgtt
       61 ctggccaaga tcgcggcgga gtatgtgctg cgctggctgc cgcccggcac ccacgactgg
      121 aaccgcttcg tagaaccgca cacacttcaa gcgatgcttg aggaggcggg gctgaacatt
      181 ttgaaaacgc agggtgtaag cttcgatccg ttgggatgga actggcgcct gtcgaccgac
      241 ttggacgtca attatatggt cgtcagcgcc agccccaggc gctagagcaa atcaccgttt
      301 attggaatcg gtgatttgct ctagctattt gttttgtcgc gcttcttatc cgaaaaccgg
      361 tttccacttt tcgggaagcg ctccagtgct tcatggccgc ggaacgaacg tgtccgatcc
      421 gatgttcatg ttgctccagc ccggtgaact cgagagaatc cagatcgtcg agatgttgcc
      481 gttattcggg ttgtagttgc cggaccaagc cgtcaccgaa ttgcacccgg ctgcggtcca
      541 gttcaccgca aaggtgatcg cgttgctttg cgaaatccag ccaatcaagg gctgctgaat
      601 gccgaccgct tggcatccgg gtgacgcaac cgccgtgacg taataaccgg agaattgtcc
      661 agtagcctga acgttcgtaa tcgtgagagt cgagccgagc ccgttctgcc agcttgtacc
      721 ggcggctaac gtctgggctt gggacggcac cgacaatcca ccgacgagca aagcaaacac
      781 tatagcaata ctgacttttc ctgcgtgcat gactgcctcc aatcgcaatt aatagttgag
      841 agttataaca attcaactaa ttaacggcaa caattctact tgtaagatta tttcaaattg
      901 cgtcgcaggt tcaattcgcc gtttcgtcca aatccggcgg caggggcgca acgtcgacgc
      961 cctcctcgat cagttccttg gcttccttga gcgtggcttc gccgtagatg tggcgctcct
     1021 cggtttcgcc gtaatgaatc ttgcgcgcct cttcggggaa gcgcgggccg acatagtctg
     1081 cgttctcttt cacatatttg cgcatgcccg acacgaactg gcgcatcttt ttcagctcgt
     1141 ctttggcgga aacggatttt ttggtgccgg cgacggcggg cgccatgatc gccttctcga
     1201 ctttgcgcga ggtgcatatg gggcagatca gcttgccggt cacggcttgg tcgtcgaagg
     1261 cggcactgct ctgaaaccag ccttcgaatt cgtggccgct gccgcaccta agattgtaaa
     1321 caatcacgtt cctgcgcctg gcacgctctt cgacacgaaa cgatgggccg aaagccccgg
     1381 cttcgcaagc cgttaactct gtagaggctt agcgagacgt ttctcgaagc agatgctttt
     1441 gggatcgccg acgtaaggcc cgaagaccgg aatctccgtc cagccgcgcg aacgatacag
     1501 gccgagcgct tcggtcatct gccgtccggt ttccaggatc gccctcgaaa aaccgcgccg
     1561 gaaggcttcc gcttcgagaa agtcgagcac cgcgcgcgcc aagccgcggc cgcgcaatgc
     1621 cggaagcaca tacatgcgct tgatctcggc cgagccctcg aagggacgga atgcgccgca
     1681 ggcgaccggc atgtcgttta catagcagac tacgaacacg ccgccggccg cttcaaattc
     1741 cgcaacgtcg atgccgttgg tcggctcgcc cggatagcgg cgaacgatgt cttcgtcgag
     1801 cgcagcgatg aggctttgag cggcagcgga gccggggctt tcgatacgga ggatcatgac
     1861 acttcgaaag gcttttcgtg cttgaggttc ggtatccgcg cgcgcgcatc ggcggcaagt
     1921 gcagggtcga tgtctgcgac gatcacgccg ggatcggttc cggcctccgc caacacctcg
     1981 ccccaaggcg cgacgatcag cgaatggccg taggttttgc ggccccttgc atgtgtgccg
     2041 ccctgcgccg gcgcgagcac gaaagcgccg ttttcgatgg cgcgggcgcg caacaacaca
     2101 tgccaatgcg ccttgccggt ggtttcggtg aaagcggatg gaatagcgag cagaaatgcg
     2161 cccgcctggg cgagcatccg gtaaagccgg ggaaaacgca gatcgtagca gacgctgaga
     2221 ccgatcctac cccaaggcag atcggcggtg acggcgcggc cgccgccggc aacggtcttg
     2281 gactcgcggt atttctcacc ggacggcagg tccacgtcga acaaatgaat cttgtcgtag
     2341 cgcgcggcga tggcgccttt gggatcgatc aggaatgagc
//
LOCUS       DASWPU010000070         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_86052,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000070 DASWPU010000000
VERSION     DASWPU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86052"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VGC27_01505"
     CDS             complement(<1..461)
                     /locus_tag="VGC27_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0281280.1"
                     /translation="MSKSVRVILILLLVTAGILNQADRQIIALLKPVMEAELGWSDAV
                     YGTLVAAFQLAAALAFPLAGWFVDRVAPKWANALAVGSWSIAAAAHGIASSFGQFLTA
                     RVTLGATEALGTPITIKTLAVMFQDARDRSFYLGLMGGVGGIGAITVPFIVP"
     gene            complement(517..>1730)
                     /locus_tag="VGC27_01510"
     CDS             complement(517..>1730)
                     /locus_tag="VGC27_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0281281.1"
                     /translation="SAAAAVKQITDHGVLDFDQYSQEVRVASPKGQFIDYVVGLYYLH
                     AVDSETYQRTAVAPAGTSNTGIAHYGVLNDNYSLFGEATVNFTDTFRAIAGVRVVGDH
                     LSRFHYRNSTAPDSGIAASSPYNADEVDKIGYADRFGLQYDLTDGITSYFTYSRGYKG
                     PAYNVFFNMVPATQGVVLSPETSSSFELGLKARLFDNKLSVNLAAFHTTFDNFQANFP
                     NLINGTVVTNLVNAGTVLTRGVESDFTANITDYLTVGGALAYTDAYVDETNIVGRNWP
                     IAGKPLPFTPDWKLSLRANYHVPLDSRFALDFGTDGRWQSKVQYDLSESPDTIEQAFG
                     VWNGKITLSDSEANWSLSLLVKNILDQHYATYLQASGATAAPGTPGYVVRWVPRDDGR
                     YVGVNIRKTFN"
BASE COUNT          342 a          514 c          564 g          310 t
ORIGIN      
        1 ggcacgataa acggcacggt gatggcgccg atcccgccga ccccgcccat cagcccgaga
       61 tagaacgagc ggtcccgcgc atcctggaac atcacagcca gggtcttgat cgtgatcgga
      121 gttcccagcg cctcggttgc gcccagggtc acgcgcgccg tgaggaactg cccgaacgaa
      181 ctggcgatgc cgtgcgccgc ggcggcgata ctccacgatc ccacggcgag cgcgttcgcc
      241 catttcggcg cgacgcgatc cacaaaccac ccggccagcg gaaaggccaa cgcggcggca
      301 agctgaaagg cggcaaccag cgtgccgtac accgcatcgc tccagccgag ttcggcctcc
      361 atcaccggct tgagcagggc gatgatctgg cgatccgcct ggttgaggat gccggcggtg
      421 acgaggagga gaatcaggat tacgcgaacc gatttcgaca cggcactcct ttgcaatgaa
      481 gacggccggg ttgccgcaac cgttctccat cccagatcag ttgaaagtct tgcggatgtt
      541 cacgccgacg taccggccgt cgtctcgcgg cacccagcgg accacatagc ccggcgtacc
      601 aggcgccgcg gtggcgcccg atgcctgcag ataggttgcg tagtgctggt cgagaatgtt
      661 cttgacgagc agactgagac tccaattcgc ctcgctgtcg ctgagcgtga tcttgccgtt
      721 ccagacgccg aacgcctgct cgatcgtgtc cggcgattcc gaaaggtcgt attgcacctt
      781 gctctgccag cggccatcgg tgccgaagtc gagggcgaag cgcgaatcca gcggcacgtg
      841 gtaattggcc cgcaagctca gcttccagtc cggcgtgaac ggcagcggct tgccggcgat
      901 gggccagttg cgaccgacga tgttggtctc gtcgacatag gcgtcggtgt aggccagcgc
      961 gccgccaaca gtcagatagt cggtaatgtt ggcagtgaag tcggattcga cgccccgggt
     1021 cagcaccgtg cccgcgttga cgaggttggt gacaaccgtg ccgttgatca ggttggggaa
     1081 gttagcctgg aagttgtcga aggtggtgtg gaaggccgcc agattcaccg ataacttgtt
     1141 gtcgaacagc cgcgccttga ggccaagctc gaaggaactc gaggtctccg gcgatagcac
     1201 caccccttgc gtggccggaa ccatgttgaa gaaaacgttg taggccggtc ccttgtagcc
     1261 gcgggaatag gtgaaatagg aggtgatgcc gtcggtcagg tcgtattgca ggccaaagcg
     1321 atcggcatag ccgatcttat ccacctcgtc agcgttgtac ggcgaggagg ccgcgatgcc
     1381 ggaatcgggg gccgtcgaat ttcgataatg aaaccgggaa agatgatcgc cgacaacgcg
     1441 cacgcctgcg atggcgcgga acgtgtcggt gaaattgact gtcgcttctc cgaacagcga
     1501 atagttgtca ttcagaacgc cataatgggc gatgccggtg ttgctcgtgc cggccggggc
     1561 cacagccgtg cgctggtagg tttccgagtc gacggcgtgc agataataga gcccgaccac
     1621 gtaatcgatg aattgcccct tgggcgaggc cacacggact tcctgcgaat actggtcgaa
     1681 gtccaggacg ccgtgatccg tgatctgctt gacggcggcg gcggccgacg
//
LOCUS       DASWPU010000071         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_6670, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000071 DASWPU010000000
VERSION     DASWPU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6670"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VGC27_01515"
     CDS             complement(<1..230)
                     /locus_tag="VGC27_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281282.1"
                     /translation="MKRSAVAVVVGYTKREGLLRKSFAPLRRLKQAGVIDRILYVTWD
                     KPELDAWLADAAAMPEVELVRVPEPEVSGKPY"
     gene            complement(1489..1878)
                     /locus_tag="VGC27_01520"
     CDS             complement(1489..1878)
                     /locus_tag="VGC27_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281283.1"
                     /translation="MQDAADHATVVHAIFAAHIRRQVRLDLTPLLIAQPKQVVPHPNA
                     SRITKQRNHQLIQPTMTLLSVGPRTLVANIAKRLKLLAYNFLSFYADFVLFRFGVGCF
                     PAQMLCRFQLLLIASSNYATAIGAGFP"
     gene            1813..2516
                     /locus_tag="VGC27_01525"
     CDS             join(1813..2091,2094..2516)
                     /locus_tag="VGC27_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611329.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0281284.1"
                     /translation="MPTDVRGKDRVDDRRVISGILHVLKSGCRWCDCPPEYGPYTTIY
                     NRFVRWAERGVWERLFRKLAERGRSTHTQMIDSTHVKAHRSASGAKRGNKQAIGRSRG
                     GRNTKIHAIADAKGRLLSILLTGGQAHDCPPSQRLIRRTKIAKKLLGDKAYDSEELRL
                     WLKARGTKPVVPNRSNRRQPFSFDKKSYKQRHRIENAFCRLKDFRRIATRYDRLARNF
                     LASVCLVAAIVWWTL"
     gene            complement(2707..>2931)
                     /locus_tag="VGC27_01530"
     CDS             complement(2707..>2931)
                     /locus_tag="VGC27_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281285.1"
                     /translation="EKVEAGPKGGTIAFRHNVFADPLALVRFIGAQHGSMKVRPDQKI
                     VVSRDWPTPEARLKGVQAVLSQLVKLAAAA"
BASE COUNT          616 a          894 c          844 g          577 t
ORIGIN      
        1 tggtacggct tgccgctcac ctccggttcc ggcacacgca ccagctccac ttccggcatg
       61 gccgcggcgt cggcaagcca tgcgtcgagt tcgggtttat cccatgtcac atagagaatc
      121 cggtcgatga cgcccgcctg cttcagccgc cgcaaaggcg cgaaactttt gcgtagaaga
      181 ccctctcttt tggtgtaccc gacaacgacc gcaacggcag accttttcat cacaaacctc
      241 tttcgatgcc ggtcagccga acggacagca tctttcgccc gacgccgcag cctttcttcg
      301 gccgacggaa tatcgtgaaa aaaccttaac cgtggttgaa gagccgggta accgaagatt
      361 gagacgcacg tcacgccgct tgcgccatat ccgagcaagg gccggaataa cagaaggcgg
      421 gcttcgtgag agcggcatgc ggccgccctg gaaaacccgc cttcggcgcg aagctacggc
      481 cggcaaacgg aatccgctag ccgcggaaac cgacaagcac ctttgcttct ccccgggccg
      541 cgcgaaacaa gcgttgttgc gatccgccgc cgcttgggcg gccccttcgc cggcttgctc
      601 cgctcaggtg cgcacgagct cgccccctgg ccgtgtatgg tctcgcgcct gctttataag
      661 ggaatccagc agccctctat gattcccgcc tgcgccatct ccttcccaaa atcgacgcac
      721 atccacagcg gatgcacttg tgcgaaatag gtattgaatc ccgtatttca agggcgcgcg
      781 gaaaatttaa tggtgcaaac gcgaggcatt atattctgtg tacagatgag gtgcgcgaga
      841 cgcggcggcg tctttcgtca gatgcggagg ttagtctgtg agataaaaac gtttgtcatt
      901 cccggccgag cgatgcgaga gcatcgcgag gggaagggaa tccatgagct tacgcaggct
      961 tctggatggt tcatacgctg tcgctatgaa ctccctcgcg ctacgcgctc gccgggcatg
     1021 acaagttggt tcaatcgaaa acgataacgc cccatgagat tttttccctt tgccggccgc
     1081 tgccggttct caacctgacc ccgaactacc cgattcgcat gacgtggacg gcgttggcga
     1141 tgacatcggc ggggcgtttt tgtccctgag ggcctttagg cattagcgtt catatctggt
     1201 caccagcttg accttttcgc acatcgcaca tggccccttt tcgcgccgaa agtcgcgggt
     1261 taggccaaat gcgcttgtaa taggattgat ctggtttctt ggggtgatgt gtagaacccc
     1321 agcaatgcaa tcgtcgcatt gaaaatgcgg cgcttcgctt ttcaggtaat caaatacctg
     1381 ttccggcacg gactggcctt ggggtccctt gggcatgggg atagcatgcc accgccgcag
     1441 ggtcatggcg aggtcatcat ggagcgcaaa ccgttcgatt gccccgtatc aggggaaccc
     1501 tgcgccgatg gccgttgcgt aattggacga tgctatcaac agcaactgga agcgacagag
     1561 catttgcgcc ggaaagcagc cgacgccgaa gcggaagaga acgaagtccg catagaagct
     1621 gagaaaattg tacgcgagaa gcttaaggcg cttggcgata ttcgctacca aagtcctagg
     1681 gccaacactc aataaagtca ttgtcggttg aatcagttgg tgattccgtt gcttcgtgat
     1741 tcgggaggca ttgggatgcg gaacaacctg ttttggctga gcgatgagca atggcgtcag
     1801 atcgagccgc acttgccgac ggatgtgcgc ggcaaagatc gcgtggacga ccgtcgcgtg
     1861 atcagcggca tcctgcatgt tctgaagagc ggctgccggt ggtgcgattg cccgccggaa
     1921 tacggcccct acacgacgat ctacaatcgc ttcgtgcgct gggccgagcg cggtgtgtgg
     1981 gagcggcttt tccgcaaact ggcggagcgt ggccgatcca cccataccca gatgatcgac
     2041 tcgacgcatg tcaaagccca ccgctcggca tcgggcgcaa aaagggggaa cttaagcagg
     2101 cgatcggccg ctcgcgcggc gggcgcaaca cgaaaatcca cgcaatcgca gatgctaaag
     2161 gccgccttct ttccatcctg ctgaccggcg gccaggcgca tgattgcccg ccatctcagc
     2221 gtctcatccg ccgaaccaag atcgccaaga agctgctcgg cgacaaagcc tacgacagcg
     2281 aagaactgcg actatggctg aaggcgcgcg gcaccaaacc cgtcgtgccc aacagatcga
     2341 accgcaggca gcccttcagc ttcgacaaga aatcctacaa gcagcgacac cgtatcgaga
     2401 acgccttctg ccgcctcaag gacttccggc gcatcgctac ccgctacgac aggctggcaa
     2461 gaaacttcct cgcctccgtc tgcctcgtcg ctgctatcgt gtggtggact ttatgagtct
     2521 gggccctaga tatgtggcgg aacttgcgca caacccaaag gtcgttgccc tagcaagaaa
     2581 gcggagggcg gaaattcggg ccctccccga agtcaaactg taacagtgcc cttaatcaat
     2641 ccccctggat ccccttccct cgccgcgcca agtgcgcggc tcgccgggga tgacagttgc
     2701 gttcgttcaa gcggcagcgg ccagtttcac caactgggaa agtaccgcct gcacgccctt
     2761 gagccgcgct tcgggcgtcg gccaatcgcg cgacaccacg atcttctgat cgggacgcac
     2821 cttcatcgag ccgtgctgcg cgccgatgaa gcgcaccagc gccagcggat cggcgaacac
     2881 attgtggcgg aaggcgatgg tgccgccctt cggtcccgcc tcgaccttct c
//
LOCUS       DASWPU010000072         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_67961,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000072 DASWPU010000000
VERSION     DASWPU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67961"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..946
                     /locus_tag="VGC27_01535"
     CDS             347..946
                     /locus_tag="VGC27_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0281286.1"
                     /translation="MAPISKERAQWLARHVLPHEAALRAWLRGKSTLGFEADDVVQET
                     YTILATKADIDTIRNPKAYMFQVAYSVILQQLRHARVVPITAVADFAGLEAVLDAPSP
                     EQAVVARQELAILMRAIEAMPRQTRQAFILRRIEGLSQAQIARHMRLSEHTVEKHIAR
                     GIKLLLAQFGHGGNASLAASMKETEPETRDDPKANCRIH"
     gene            1059..1949
                     /locus_tag="VGC27_01540"
     CDS             1059..1949
                     /locus_tag="VGC27_01540"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0281287.1"
                     /translation="MRAEAALIGCGADEILVIPPDLRPSRVSPSRRWVIGGTAAGLAA
                     VCVGVALPFRSAHRLRSFTSELGEIRHVPLEDGSRLTMNTDSTLSVDYARDRRTIRLM
                     RGEAFFEVAKDPARPFIVVGPNAQVRTVGTAYSVRLTGGDEMRVLVAAGKVAIETVSE
                     TAGAISTLEQLWRSGAEGARAVFVEAGQVAKVWQGKGGEEGNGLLVAVASLAKDTPQR
                     ALLWREGQLYLEGQTLAQAVSEFARYSRRRIVIADPALGETRVTGLFAAGDPEGFARA
                     IALSLGAHVVRRADSIVLAR"
     gene            2083..>2830
                     /locus_tag="VGC27_01545"
     CDS             2083..>2830
                     /locus_tag="VGC27_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0281288.1"
                     /translation="MISNVLWRSLMAGAALSVICSVTEATAQPRHFDLPAQPAVKSIP
                     MFAVQAEVQIVAPADKLAGIETGAIKGDFERADALQRLLRGTSLTVASDDGKVVVLRM
                     AADQSRLAAPPLQQTAAASVDNIETVTVTAQRRSQRAVDVPFALTAYRGETLKEMGAV
                     SLRDVSLVTPGLLVEDQSPNNPIFVVRGITSSGGDSFNEPRVSVFQDGVPISKSRGSF
                     VELFDIDRVEVAKGPQSTLYGRGALIGGVNV"
BASE COUNT          529 a          835 c          924 g          542 t
ORIGIN      
        1 gaggaggaca agttgtcctg tcacgaggcg gcaaggccaa tgggcgggga tcggtgttca
       61 gtgctgaagc cggggagggc ttggcttcaa cggttgatgg agcgcgatgt caatcggctg
      121 caaaacggat cccgtatcgg ctttcaaagt tgatcccacc tatgtgtgag cgaagcacgc
      181 gagggtagct gtacggctta taggcggagg tttaggcgcg cgtacggccc agggcgcgcc
      241 catccgcaaa cggcatatat aatctttttg ttacacctgg cggcgtcacg catccgccgc
      301 atctagctcc tagggttcct tccgacgatc agcaaaaagt agaaccatgg cgccaatcag
      361 caaagagcgt gcacaatggc tggcgcgcca tgtgctgccc catgaggccg cgctccgggc
      421 gtggctgcgg ggaaaatcca cccttggttt cgaggccgat gatgttgttc aggaaaccta
      481 tacaattttg gctacgaagg cggacatcga caccatccgc aatcccaaag cctatatgtt
      541 ccaggtcgcg tattcggtga tccttcagca attgcgtcat gcgcgggtgg tgccgatcac
      601 ggcggtggcg gatttcgccg gtctcgaagc cgtactcgat gcgccttcgc ccgaacaggc
      661 cgtggtggca cgccaggaac tggcaatcct catgcgggcg atcgaggcaa tgccgcgcca
      721 gacgcgccag gcctttatcc tgcgccgtat cgagggcctg tctcaggcac agatcgcccg
      781 ccatatgcgt ttgtcggagc atacggtgga aaaacatatc gcgcgcggca tcaaattgct
      841 gttggcccag ttcgggcatg ggggaaacgc cagcttggct gcatctatga aggaaacaga
      901 gccagagacg cgtgacgacc caaaagcaaa ctgcagaatc cattgacgag gcggccgccc
      961 ggtgggcggc aagacgcgat gctggtccct tggctgcggt agaggaagcc acattcgacg
     1021 cttggcttca gagtgactcc cgctgcctgg gtgccttcgt gcgggccgaa gccgcgctga
     1081 tcggttgcgg cgcggacgaa atcctcgtca ttcctcccga tctgcggcca agccgggttt
     1141 cgccaagcag gcgttgggtg ataggcggga cggccgccgg tcttgcggcg gtttgcgtcg
     1201 gcgttgcgct accttttcgt tcggcacatc ggctgcgcag cttcacctcc gaactcggcg
     1261 agatccggca tgttccgctc gaagacgggt cacggctgac catgaacacg gactcgaccc
     1321 tcagcgtcga ttatgcacgc gaccggcgca ccatccgtct gatgcgcggt gaagcattct
     1381 tcgaagtcgc caaggatccg gcgcgtcctt tcatcgtggt ggggccgaat gctcaggttc
     1441 gcacggtcgg cactgcctat tccgtccgct tgacaggggg tgacgagatg cgggtcctgg
     1501 ttgccgccgg caaggtggcg atcgaaaccg taagcgaaac tgcgggcgcc atctccaccc
     1561 tggagcagct ttggcgcagc ggcgctgagg gggcgagggc tgtttttgtc gaggccggac
     1621 aggtagcgaa ggtgtggcaa ggcaagggcg gcgaggaagg caacggcttg ttggtcgccg
     1681 tcgcctcttt ggccaaagat accccgcagc gcgcgctttt gtggcgcgaa ggacaacttt
     1741 acctggaagg gcaaaccctg gcgcaggctg taagcgaatt tgcccgctac agccgccggc
     1801 gcatcgtgat tgccgatccc gcgctgggcg aaacgcgcgt gacgggcctg tttgccgcag
     1861 gcgacccgga aggcttcgcg cgcgccatcg ccctcagcct gggcgcccat gtcgtgcggc
     1921 gggcggacag catcgtgctt gctaggtagc gctatagcgc tggacgcacc gtttttcggc
     1981 gacagcaaga tgaatttctt ttgacagatg gcggaaggcg tgacggcgcc catctaggaa
     2041 acaaggcgcg aaactcgcgc catccagaat gtgggggtaa atatgatttc gaatgtcctg
     2101 tggcgcagcc tgatggcagg tgcggcgctt agcgtgatct gttccgtcac ggaagcaacg
     2161 gcccagcctc ggcattttga tttgccggcc cagcccgccg tgaaatcgat tccgatgttc
     2221 gccgtgcagg cagaggtcca aatcgtcgcc ccggccgaca agctcgccgg aatcgaaacc
     2281 ggcgccatca aaggcgattt tgagcgcgcc gatgccctgc agcgattgtt gagaggtaca
     2341 tctctgacgg tcgcctccga cgatggaaaa gtcgttgtgt tgcgcatggc cgccgaccaa
     2401 tcgaggctgg cggcaccgcc gttgcaacaa acggccgccg ccagtgtaga caacatcgag
     2461 acggtgaccg tgacggccca gcgccgcagc cagcgtgccg tcgatgtacc cttcgcgctg
     2521 accgcctatc gcggcgaaac gctgaaggaa atgggggcgg tgtccctgcg cgacgtctcg
     2581 ctggtgacgc cggggctgtt ggtcgaagac cagtcgccca acaatcccat cttcgtggtg
     2641 cgcggcatca cctcgtccgg cggcgattcg ttcaacgagc cgcgggtctc cgtcttccag
     2701 gatggcgttc cgatctcgaa atcgcgcggt tccttcgtcg agttgttcga catcgaccgg
     2761 gtcgaagtcg ctaaggggcc gcaatccact ctttatggcc gtggcgcgct gatcggcggg
     2821 gtcaacgtca
//
LOCUS       DASWPU010000073         8100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_533, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000073 DASWPU010000000
VERSION     DASWPU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VGC27_01550"
     CDS             complement(<1..444)
                     /locus_tag="VGC27_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY0281289.1"
                     /translation="MTYRLLLLPGDGIGPEVLEETRRVLGWYSANRNLAFTTETDLVG
                     GIAYDTHGAPATDAMMAKALKADAVLFGAVGGPQWDKLPFHLKPEQGLLRLRGDLGLF
                     ANLRPALCFEALKEASTLKPEIVSGLDILIVRELTGGVYFGLPKET"
     gene            complement(541..1146)
                     /gene="leuD"
                     /locus_tag="VGC27_01555"
     CDS             complement(541..1146)
                     /gene="leuD"
                     /locus_tag="VGC27_01555"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY0281290.1"
                     /translation="MEAFKQLSGVAAPLPMINVDTDMIIPKQFLKTIKRTGLGKALFH
                     EMRTKPDGSDNPDFVLNKPAYKNAQILIAGANFGCGSSREHAPWALLDYGIRCVIAPS
                     FADIFYNNCFKNGILPIALPQTEIDKLLDDAARGANAIVSVDLEKLEIRGPDGGCIKF
                     EVDPFRRQCLLNGWDDIGLTLQNENKITSFEAARKAQMPWL"
     gene            1335..1646
                     /locus_tag="VGC27_01560"
     CDS             1335..1646
                     /locus_tag="VGC27_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin-associated ferritin-like protein"
                     /protein_id="HEY0281291.1"
                     /translation="MSSEGIHVPYEKLSRHTLHMHYAITSLKEELEATDWYRQRADDT
                     EDEKLKAILLHNADEEIEHASMLLEWIRRNDPRFNKELREYLFTEGPITELEGKAMGR
                     K"
     gene            complement(1678..1941)
                     /locus_tag="VGC27_01565"
     CDS             complement(1678..1941)
                     /locus_tag="VGC27_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEY0281292.1"
                     /translation="MVTSAIRTDPRVADVRVSSTTLTVMLRDGREISAPLDWFPKLKA
                     ADDEARSVWEPAAAGHGIHWPRINEDLSIDGLLQGSRAPRLQN"
     gene            complement(1916..2161)
                     /locus_tag="VGC27_01570"
     CDS             complement(1916..2161)
                     /locus_tag="VGC27_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEY0281293.1"
                     /translation="MMPTVLLWNGYRFYFFSNEGHEPPHIHVDKNGRSAKFWLDPVEL
                     ARNFGFSQTELRVVEDKVREEQQRFFEAWHGYFGHQD"
     gene            complement(2158..2496)
                     /locus_tag="VGC27_01575"
     CDS             complement(2158..2496)
                     /locus_tag="VGC27_01575"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY0281294.1"
                     /translation="MSGTWPTELRLDGAKTKLTVSFENGERYELPAEYLRVESPSAEV
                     QGHSPEQKQIVTGKEEVKIVGLEPVGNYAVRIVFDDGHDTGLYSWDYLGELGREHAAR
                     WAAYVKSRGA"
     gene            complement(2493..2684)
                     /locus_tag="VGC27_01580"
     CDS             complement(2493..2684)
                     /locus_tag="VGC27_01580"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY0281295.1"
                     /translation="MEPDPKLLDLLVCPVTRATLVYDREKQELISRHAGLAYPIRSGI
                     PIMLPGEARPLSDAEHDRK"
     gene            complement(2792..3445)
                     /locus_tag="VGC27_01585"
     CDS             complement(2792..3445)
                     /locus_tag="VGC27_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY0281296.1"
                     /translation="MGGNYHSLDDLPPTLGVLPLTGVVLLPRGQLPLNIFEPRYLALV
                     DAAMSGSRLIGMIQPTQHEDKVLKPSLSAIGCAGRLTSYRESEDGRYLITLTGVCRFR
                     IAEELQADTPFRRVRAEFAPFLADLIAADEHDFPRDRLLGALKDYLSRRDMKADWRSV
                     LGAAPETLVNALAMLCPFEPAEKQALLEAPGWLERVDTLIALLEMANAGPTGPISLN"
     gene            complement(3455..4396)
                     /gene="trxA"
                     /locus_tag="VGC27_01590"
     CDS             complement(3455..4396)
                     /gene="trxA"
                     /locus_tag="VGC27_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0281297.1"
                     /translation="MALIGTSGQPLGGQNGSRPAAAGPTVKETSLATFAADVLEASRE
                     VPVIVDFWAPWCGPCKQLGPALEKAVNAAKGAVRLVKVNVDENQEIARQLRIQSIPTV
                     YAFKNGQPVDGFMGAIPESQVKTFVETLIGGGHGGEDHAAEILAAADAAFAAGDLSQA
                     AQAYGHVLQDEPGHPKAAAGLARCYLKSGDIERAKTTLQLVRPDDAADEAVRAVEAEL
                     KLREQAANPGDAGALKARLAADPNDHQARYDLALALDARAERDAAIEELLELVRRDRK
                     WNDDAARKHLVTLFDAMGPADPRTVAARRKLSSILFS"
     gene            complement(4777..5301)
                     /locus_tag="VGC27_01595"
     CDS             complement(4777..5301)
                     /locus_tag="VGC27_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEY0281298.1"
                     /translation="MANELPSSEAREEALYERLRALGIGWKTYEHIPVFTVAEADAVH
                     GSVPGVHTKNLFLKDKKGGLWLVVLRDRLRVDLNALAKQLGAPRFSFGSAELLIATLG
                     VEPGSVTPFALKNDTAHNVRPILDEGMLAQGPLNFHPMRNDRTTAILPGDLLGFVRAC
                     GHDPIIAALPEMAP"
     gene            complement(5301..6164)
                     /gene="speE"
                     /locus_tag="VGC27_01600"
     CDS             complement(5301..6164)
                     /gene="speE"
                     /locus_tag="VGC27_01600"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEY0281299.1"
                     /translation="MPANHFKERLHKGYAQAMELVGKPLAEEKSRYQRIKIFDTVANG
                     RVLVLDDVVQLTTRDESAYAEMLTHLPILEHGKVERAMIVGGGDLSIAEEILKHKGVK
                     EVVLVDIDGRVVELCRQHFAEINARAFKNKRLKIEIADAFAYLGRKENKARFDLIVGD
                     RPDPIGPGKALFGETFYDRIKRALKPGGFATFQTGVPFYQPWEVTEALAELKRFFPRS
                     GLYLTVVPTYIGGFMALSWAGKGAQLGTPKGLKRAAAAYKRKKLKTDYYNPAVHAASF
                     ALPEWIGRLVP"
     gene            6492..6968
                     /gene="speD"
                     /locus_tag="VGC27_01605"
     CDS             6492..6968
                     /gene="speD"
                     /locus_tag="VGC27_01605"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEY0281300.1"
                     /translation="MARLELVAAIKEAKAPRARASARTVPVPIPPGDEDRKDHFITRN
                     GLTYAGSHLIIDLWDAEGLDDRDRIETALIDAVKAAGATLLHIHLHTFTDGGGISGVA
                     VLAESHISVHTWPERKYAAFDVFMCGEAEPRKALSVFKAAFNPGRLVIGEHKRGVL"
     gene            7130..>8100
                     /locus_tag="VGC27_01610"
     CDS             7130..>8100
                     /locus_tag="VGC27_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0281301.1"
                     /translation="MRYDACIIGAGADGLAAAATLAARGLKVTVVERDSQCGGRCATR
                     EFHPGFRASPYADEVPAIPSGIFWSLDLARRGVLLMPAPASPPGGEALRRAIADRVVA
                     DAGESAKRTLLPSRAPYVRWPGEELAGRTLLDLVEELSTDFAGRARLLAALLNGRVCD
                     PALAGSALHLLAGAPGGMAAGGLGRLGEALRAAAAEAGAEIVCGLEATDIKRKGRRAA
                     GVGLADGSDIAARAVISTLDLKRTFLSLFTWSELPRALVDRVGAFRPAPGTARLLLAL
                     EAPPDLPAGMETLRAPIHAAPDTLDEAYRAWRRGAVPERPPAMLRLVS"
BASE COUNT         1394 a         2547 c         2654 g         1505 t
ORIGIN      
        1 ggtttccttg ggcaagccga aatagacccc gccggtcagc tcgcgcacga tcaggatgtc
       61 gaggccggaa acgatctccg gcttcagcgt ggaggcttcc ttcagtgctt cgaagcacag
      121 ggcggggcgc agattggcga acaatccaag gtcgccgcgc agccggagca aaccttgctc
      181 cggcttcagg tgaaacggca gcttgtccca ttggggaccg ccgaccgcgc cgaacagcac
      241 cgcgtcggcc ttcagcgcct tcgccatcat cgcgtcggtc gcgggcgcgc catgggtgtc
      301 gtaggcgatg ccgccgacca ggtcggtctc ggtggtgaaa gcgagattgc ggttggcgga
      361 ataccagccg agcacgcggc gcgtttcttc gagcacttcc ggcccgatac cgtcgcccgg
      421 aaggaggagc aatctatagg tcatgacggt tgatccccgt ttgggaagtt ggaaaatcgc
      481 acgcggaggc gcggagagat agcacgcaag ctccgcgatc tccgcgtctc cgcgtgaacc
      541 ctaaagccac ggcatctgcg ctttgcgcgc cgcctcgaag ctagtgatct tgttttcgtt
      601 ctgcaacgtc aggccgatat cgtcccagcc gttgagcagg cattggcggc ggaagggatc
      661 gacctcgaat ttgatgcagc cgccgtccgg tccgcggatt tcaagtttct ccagatcgac
      721 cgagacgatg gcgttggcgc cgcgcgcggc gtcgtcgagc agcttgtcga tttccgtttg
      781 cggcagggcg atgggcagaa tgccgttctt gaagcagttg ttgtagaaga tgtcggcgaa
      841 gcttggcgcg atcacgcagc ggatgccgta atcgagcagc gcccagggcg cgtgctcgcg
      901 gctcgagccg cagccgaaat tcgctccggc gatcaaaatt tgggcgtttt tgtaggccgg
      961 tttgttgagc acgaagtcgg gattgtcgct gccgtccggc ttggtgcgca tctcgtgaaa
     1021 cagcgctttt cccaggccgg tgcgcttgat agttttcagg aattgcttgg ggatgatcat
     1081 gtcggtatcg acattgatca tcggcagcgg cgccgcgacg ccggacagtt gcttgaaagc
     1141 ttccatggca cgggggaact taatagctgc gaccgcttat cacaagttag aaccgttcca
     1201 aattgcttct ttttgcgcta tggtatcggc aacaaggtgg gtccatgttc aaatttcttt
     1261 tccgccgccg cgaagcgcgc gttcctgtcg cagaggctcc ggccgctccg gcgccaactc
     1321 aaggagttcc gtccatgtcg agtgaaggca tccacgtccc ttacgagaag ctgtcgcgcc
     1381 atacgctgca tatgcactac gccatcactt cgctgaagga ggagctggag gccaccgatt
     1441 ggtaccgcca gcgcgccgac gacacggagg acgaaaagct caaagcgatc ctgctgcata
     1501 atgcggacga ggagatcgag cacgcctcga tgctgctgga gtggatcagg cgcaacgatc
     1561 cgcgcttcaa caaggaactg cgcgagtacc tcttcaccga aggccccatc acggagcttg
     1621 aaggcaaggc catggggcgg aaataggctt caggagcggc cgccgcgcgc catggtttta
     1681 gttttgaagc cgaggcgcgc gcgagccctg caacagcccg tcgatgctga gatcctcgtt
     1741 gatccgcggc cagtgtatgc catggccggc cgccgcaggt tcccagacgc tgcgggcttc
     1801 gtcgtcggcc gccttgagct tcgggaacca atcgagcggc gcgctgattt cgcggccgtc
     1861 gcgcaacatg accgtcagcg tcgtggaaga tacgcgcacg tcggcgacgc gcggatcagt
     1921 cctgatggcc gaagtaacca tgccatgcct cgaagaaacg ctgttgctct tccctgacct
     1981 tgtcttctac cacgcgcagc tcggtttgtg aaaatccgaa attgcgggcc aattctacgg
     2041 gatcaagcca gaatttggcg cttctgccat ttttatcgac gtggatgtgc ggcggttcgt
     2101 ggccttcgtt gctgaagaaa tagaagcggt aaccattcca aagcaggacg gtcggcatca
     2161 tgctccccgc gatttcacat acgccgccca gcgcgccgcg tgttcgcggc cgagttcgcc
     2221 gagatagtcc caactgtaaa gcccggtgtc gtggccgtcg tcgaagacga tgcgcacggc
     2281 gtaattgccg accggttcga ggcccacgat cttcacctct tccttgccgg tgacgatctg
     2341 cttctgctcc gggctgtggc cctgcacctc ggcgctgggc gattccacgc gcagatattc
     2401 cgccggcagt tcgtagcgct cgccgttctc gaaactgacg gtcagtttcg tcttggcgcc
     2461 gtcgaggcgc agttcggtgg gccaggtgcc gctcacttgc ggtcgtgctc cgcgtcgctg
     2521 agcgggcgcg cctcgcccgg caacatgatc gggatgccgc tgcggatggg ataggcaagg
     2581 ccggcgtgcc ggctgatcag ttcctgcttt tcgcggtcgt agaccaaggt ggccctggtc
     2641 accgggcaga ccagaaggtc gagcagcttg gggtcgggtt ccatagacac tacctcgtca
     2701 cgcacaccct tcgtccttcg gcaagctcag gatgacgaag ctcagggtga gggaaaagct
     2761 aaaccttcat gctgagcttg tcgaagcatg ctcagttcag cgaaattggt cccgtcggtc
     2821 ccgcgttcgc catttccaag agggcgatca gcgtatcgac gcgctccagc cagcctgggg
     2881 cttcgagcag cgcctgcttc tccgccggtt cgaacggaca caacatggcc agcgcgttga
     2941 ccagtgtttc gggcgccgcg cccagcacgc tgcgccaatc ggctttcatg tcgcgccggg
     3001 agagataatc cttgagcgcg ccgagcaggc ggtcgcgcgg gaagtcgtgc tcgtcggcgg
     3061 caatcagatc ggcgaggaac ggcgcgaatt cggccctgac ccggcggaac ggcgtatcgg
     3121 cctgcaattc ttccgcgatg cggaaccggc acacgccggt gagcgtgatc agatagcgtc
     3181 cgtcctcgct ctcccggtat gaagtcaggc gcccggcgca gccgatggcc gaaagcgacg
     3241 gcttcaacac tttgtcttcg tgctgagtgg gctggatcat accgatcagg cggctgccgg
     3301 acatcgcggc atcgaccagg gccagatagc gcggctcgaa aatgttgagc ggcaattgtc
     3361 cgcgcggcag cagcaccacg cccgtcaacg gcaggacgcc gagcgtcggc ggcaggtcgt
     3421 cgagcgagtg gtagtttcct cccacggccg ggcctcagga aaaaaggatg gaagagagtt
     3481 tgcgcctggc cgcaacggtg cgcggatcgg cggggcccat ggcgtcgaac agcgtcacca
     3541 gatgcttgcg cgcggcatcg tcgttccatt tgcggtcgcg ccgcaccagt tccagcagct
     3601 cttcaatggc cgcgtcgcgc tcggccctgg cgtcgagcgc cagcgccaga tcgtagcgcg
     3661 cctgatgatc gttcgggtcg gctgcgagcc gggccttcag cgcgcccgcg tcgccgggat
     3721 tcgccgcctg ttcgcgcaat ttgagttcgg cttcgaccgc gcgcacggct tcgtccgccg
     3781 cgtcgtcggg ccgcacaagc tgaagcgtgg tcttggcccg ctcgatgtcg ccgcttttca
     3841 gatagcatct ggccagcccg gcggcggcct tgggatgacc gggctcgtct tgcagcacat
     3901 ggccataggc ttgcgccgcc tgactcaggt cgcccgcggc aaaggccgca tcggccgccg
     3961 ccaatatctc cgccgcatga tcttcgccgc cgtgcccgcc gccgatcagg gtttccacga
     4021 aggtcttgac ctggctttcg gggatggcgc ccatgaaacc gtccaccggc tgcccgttct
     4081 tgaaggcata gacggttgga atcgactgga tgcgcaactg gcgggcgatt tcctggttct
     4141 cgtcgacgtt caccttaacc aagcggacgg cgcccttggc cgcgttcacg gctttctcca
     4201 gcgccgggcc gagctgcttg cacggtccgc accagggcgc ccagaaatcg acgatcaccg
     4261 gcacctcgcg cgaggcttcg aggacgtcgg cggcgaaggt cgccagcgag gtttctttga
     4321 cggtggggcc agcggccgcc ggccggctgc cgttttgccc gcccaagggc tgcccggacg
     4381 taccgatcaa tgccatggct gcgttttcct gtcctgcgcc gcgcaacatg ggggcaagcg
     4441 ccgcgaaaga aaagacctaa tctgcgagac aaaaaaagcc ccgcggccca gagggccgcg
     4501 ggggattcca agtttatacg cacggcgctc gtggagcgcg ggcgggatgt tacggggaga
     4561 gtcgcagcgt cgtggtgccg gtcctggccg catccgtggg cacggcaaag cccgcagcgg
     4621 cgccgatagt cacggccaac acgcacaaga tcagaaagcg cttcaacatg tccctcgctc
     4681 ctcgatgagc cgtcgggagc cattagaagc cgggaagatg gcgacatctt tccggcaaag
     4741 tggcgatatt gtggtgcgcc gcgccggcga ggcggcctac ggcgccatct ccggcagagc
     4801 ggcgataatc ggatcgtggc cgcaggcgcg cacgaagccg agcagatcgc cgggcaaaat
     4861 cgccgtggtg cggtcgttgc gcatgggatg gaagttcagc ggaccctgcg ccagcatgcc
     4921 ttcgtccagg atgggccgca cattgtgcgc ggtgtcgttc ttcagcgcga agggcgtcac
     4981 cgatccgggc tcgacgccca gcgtggcgat caagagttcg gccgagccga aagagaagcg
     5041 cggcgcgcca agctgcttgg ccagggcgtt cagatccacc cgcagccggt cgcgcaggac
     5101 gaccagccac aacccgccct ttttgtcctt gaggaaaagg ttcttggtgt gcacgccggg
     5161 cacgctgccg tggacggcat cggcttcagc gaccgtgaac accgggatgt gctcgtaggt
     5221 cttccagccg atgccgagcg cccggagccg ctcgtacaac gcctcttcgc gcgcctcgga
     5281 cgaaggtagt tcgttcgcca tcagggcacc agccgcccga tccactcggg cagggcgaat
     5341 gatgcggcgt ggaccgccgg attgtaatag tcggtcttga gctttttcct cttatacgcc
     5401 gccgcggcgc gcttaaggcc cttcggcgta cccagttgcg cgcccttgcc ggcccaggac
     5461 agcgccatga agccgccgat atatgtcggc accacggtca gataaaggcc cgagcgcggg
     5521 aagaagcgct tcaactccgc gagcgcttcg gtgacctccc agggctggta gaacggcacg
     5581 ccggtctgga aggtggcgaa gccgcccggc ttcagcgccc gcttgatgcg gtcgtagaag
     5641 gtctcgccga acagcgcctt gccggggccg atggggtccg ggcggtcgcc gacgatcaaa
     5701 tcgaagcggg ccttgttctc tttgcggccg agataggcga aggcgtcggc gatctcgatc
     5761 ttgagccgct tgttcttgaa cgccctggcg ttgatctcgg cgaaatgctg gcggcaaagc
     5821 tcgacgacgc gcccgtcgat atccaccaga accacctctt tgacgccttt atgcttaagg
     5881 atctcttcgg cgatggagag atcgccgccg cccacgatca tcgcccgctc gaccttgccg
     5941 tgctccagga tcggcaaatg tgtcagcatt tcggcatagg cgctttcgtc gcgggtcgtc
     6001 agctggacca cgtcgtcgag caccagaacg cgtccgttgg caaccgtgtc gaagattttg
     6061 atgcgctggt agcggctctt ttcctcggcc agcggcttgc cgacgagctc catcgcttgg
     6121 gcgtaaccct tgtggagacg ctctttgaag tggttggccg gcatcgtgga ctccttcatc
     6181 tggcgcttgg cggcgaacga ggcggcgctt ttagggcggc tgctcccggc tggcaagcgc
     6241 gtgcacgcgc cgctcgccga ggcggctttt gtgacaaaga gagaagcagc cgcgacacgc
     6301 ctccggcaaa aaagtttaca cttaggtgac aaaaatcttg cgtcgggggg cgatggccct
     6361 atattccgat agttcccttc ggggaacaaa gcggcgcgac ggccttccgt cgggccgctt
     6421 tcttcttgag gcgcacccct tcgcaaggag aggtgcacag gtgtcaacgc tctgagaggg
     6481 taggtgccag aatggctcga ctcgaactcg tagcggcgat caaagaagca aaagcgcctc
     6541 gcgctcgcgc ttcggcgagg accgttccgg ttcctattcc ccctggggat gaggaccgca
     6601 aggaccactt catcacacgt aacggactga cctatgcggg aagtcacctg ataatcgatc
     6661 tatgggacgc cgagggtctc gacgaccgcg acaggatcga aacagcgctg atcgatgcgg
     6721 tgaaagcggc gggcgcaacc ctgctgcaca ttcacctgca tacgttcacc gacggcggcg
     6781 gcatctcagg agttgccgtt ctggcggaaa gtcacatcag cgttcatact tggcctgaac
     6841 ggaaatacgc ggctttcgac gtgttcatgt gcggcgaagc cgaacccaga aaggccctgt
     6901 ccgtgttcaa ggcggctttc aatcccggcc gcctcgtcat cggcgaacac aagcgcggcg
     6961 tgctgtaatc agcctcaaag cgcaattaat gaagggcgga aatggtcgag aagaccgttt
     7021 ccgccctttt tcttcgcgcc cttctccgcc cggagaaaga tcggagtgca ttaactgtat
     7081 tccagtcgcg actgagcgat aatggcgccg ggcgagcagg gcggggccgt tgcggtacga
     7141 cgcatgcatc atcggcgcgg gtgcggacgg attggccgca gcggcgacgc ttgccgcgcg
     7201 cggcctgaaa gttaccgtcg tggagcgcga ttcccagtgc ggcgggcgct gcgccacgcg
     7261 cgaattccac ccgggctttc gcgcctcgcc ttatgccgac gaagttcccg ccatcccctc
     7321 cgggattttc tggtcgctcg atctggcgcg ccgcggcgtg ctgctgatgc cggctccggc
     7381 ttcgccgccg ggcggggagg cgctgcgccg tgcgattgcg gaccgcgtag tggcggatgc
     7441 cggcgaatcg gcgaagcgca ctctgctccc ttcgcgcgcg ccttatgtgc gctggccggg
     7501 cgaagagctt gccgggcgaa cgctgctcga cttggtggaa gagttgtcca ccgatttcgc
     7561 cggacgcgcc cgtctgctgg ccgcgctgct gaacggaaga gtctgcgatc cggcgctcgc
     7621 cggcagcgcg ctgcacctgc tggccggtgc tccgggcggc atggcggcag gcgggctggg
     7681 acggttgggc gaggcgctgc gtgcagcggc cgcagaggcg ggcgccgaga tcgtctgcgg
     7741 cctggaagcc accgacatca agcgtaaggg ccgtcgcgcc gccggcgtcg ggctggccga
     7801 cggcagcgac atcgccgcgc gcgccgtcat ctcgacgctc gacctcaaac ggaccttcct
     7861 ctccctgttc acctggagcg aactgccgcg ggcgctggtg gatcgcgtcg gcgcgtttcg
     7921 tccggcaccc ggaacggcgc gcctgctgct ggcgctggag gcgccgcccg atcttcccgc
     7981 aggcatggag actctgcgcg ctcccatcca tgccgcgccc gatacgctcg acgaggccta
     8041 tcgcgcctgg cggcgggggg cggtgcccga acgcccgcct gcgatgctgc gcctggtctc
//
LOCUS       DASWPU010000074         4779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_116778,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000074 DASWPU010000000
VERSION     DASWPU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116778"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1041
                     /gene="lpxK"
                     /locus_tag="VGC27_01615"
     CDS             70..1041
                     /gene="lpxK"
                     /locus_tag="VGC27_01615"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY0281302.1"
                     /translation="MRAPSFWDRRDFWSRLAVAALAPFGWLYSASVAWKARSATPYRP
                     GIPVICVGNLTVGGTGKTPIAIAIARALTARGRRPFFLSRGYGGMLHGPILVEPDHHA
                     GDVGDEPMLLADAAPVVVSRDRGEGAQLAEARGADVIVMDDGHQNFTLAKDMSLVVVD
                     AEQQFGNGFVLPAGPLREFVSQGLARADAVVIVGDGGPALTNYSGPILRAHLTHVDTP
                     DLAGRRVVGFAGIGRPEKFFRSLRAFGAEIVATKRFGDHHIYTPSEIARLKATAREAG
                     ALLVTTEKDYVRMTEVEREGITVLPIRAAIDNGKALELLLDRLCASR"
     gene            1054..2001
                     /locus_tag="VGC27_01620"
     CDS             1054..2001
                     /locus_tag="VGC27_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0281303.1"
                     /translation="MSKQAPLTFWKKLRYGAEAAAFFLLMGFFRLIGIDAASAAGGFI
                     GRHIFYRLPTADTARANLRAAYPDMDDAEREKTVREMCDNLGRVVGEYPHLDKFTLGP
                     GGRVEVSGMKNAEAAIANGKGVMFISGHLANWEALPITGNLLGYDGALVYRPPNNPFV
                     DRWISRQRAKLGPKEHISKGAQGTRRIFTLLRKGKSILMLVDQKTWEGVPAPFFGRDA
                     MTTPAPASLALKLGSILLPASAERIGGARFRVTIHEPIEFEPSGDDKRDVLALTAKIN
                     QAIESMVRAHPSQWLWIHHRWPGPRDAVKMKNKQNAVRA"
     gene            complement(1991..2839)
                     /locus_tag="VGC27_01625"
     CDS             complement(1991..2839)
                     /locus_tag="VGC27_01625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0281304.1"
                     /translation="MRLARRTFLAAGAATIVLPRLARAEVNALSFGGSTEQGGLVIGK
                     APAGAAVSVDGAAVRLSPQGNFVFGFSYDRIEPAHVVALYADGGSEARDVAPIPRQYE
                     TQSIEGLPQELVTPSPENLERIKRENALITEARTRDSGGTGFAGPFDWPFPGIVTGVY
                     GSRRIENGTPMAPHLGVDIAAPEGTPIRAPADAIVSLTGDFLVNGGFTQLDHGHGVSS
                     CYLHQSERMVKAGDKVARGDVIGLVGKTGRATGPHTHWGLAWFQVKLDPSRSTRSPVP
                     PVPPKP"
     gene            complement(2836..3060)
                     /locus_tag="VGC27_01630"
     CDS             complement(2836..3060)
                     /locus_tag="VGC27_01630"
                     /inference="COORDINATES: protein motif:HMM:NF021392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEY0281305.1"
                     /translation="MNKMEREVRPEGLAELEYLDGEYRVVKAGSFVICAVTGVQIPLD
                     ALRYWNVELQEAYATAAAALKRFQETGKTP"
     gene            complement(3124..4524)
                     /gene="xseA"
                     /locus_tag="VGC27_01635"
     CDS             complement(3124..4524)
                     /gene="xseA"
                     /locus_tag="VGC27_01635"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY0281306.1"
                     /translation="MSDLANLPELSVTELSAALKRTVEDAFPYVRVRGEISGLKFHSS
                     GHVYFDLKDDKAVLNAVIWKGTARSLKVRPEAGLEVVCTGRVSTYAGSSRYQLIVEQV
                     ELAGLGALMAMLEERKKKLAAEGLFEASRKKKLPFLPEVIGVVTSPTGAVIRDIMHRL
                     QARFPRRVLLWPVAVQGERAAGEIAAAIHGFTEFPQAGLPRPDVLIVARGGGSIEDLM
                     AFNEEAVVRAVAECSIPVISAVGHETDTTLIDFAADIRAPTPTAAAELAVPVRTELIA
                     QTLDFERRLLRAFVRSMEDRRRAVRELARVLPRAEQLFAQPRQRVDVAAERLGHALRG
                     NLGAHRRAFIAVSSLLRPAGLKNRLSLGAERTQALALRLGRNQRTRLAEFAARLEGLS
                     RLLDGVSHHAVLARGFALVRGADGTLRRRAAALIPGERLLLTFGDGVAPAVAGEGGRK
                     PKSGGKKAGGDQGDLF"
     gene            4684..>4779
                     /locus_tag="VGC27_01640"
     CDS             4684..>4779
                     /locus_tag="VGC27_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0281307.1"
                     /translation="MNVLLIGSGGREHALAWSLAASPIVSKLYCAP"
BASE COUNT          846 a         1591 c         1542 g          800 t
ORIGIN      
        1 atacgggagc caggagtctc ggcggggccg tcgccaagac gattgacgtc gtcgaaaagc
       61 tgctcgccga tgcgtgcgcc tagcttctgg gaccggcggg atttctggtc gcgccttgcg
      121 gtcgcggccc tggccccttt cggctggctc tacagcgcca gcgtggcgtg gaaggcgcgc
      181 agcgcgacgc cttaccgccc cggcattccc gtcatttgcg tcggcaatct gaccgtcggc
      241 ggcaccggca aaacgccgat tgcgatcgcc attgcgcgcg cgctgacggc ccgcggacgg
      301 cgcccgttct tcctctcgcg cggctatggc ggaatgctgc acggaccgat cctcgtcgaa
      361 cccgaccacc atgccggcga tgtcggcgac gaaccgatgc tgctggccga cgccgcgccc
      421 gtcgtcgttt cgcgcgaccg cggcgaaggc gcgcaactgg ccgaagcgcg cggcgccgac
      481 gtcatcgtga tggacgacgg ccaccagaat ttcaccctcg ccaaggatat gtccctggtc
      541 gtcgtcgacg ccgagcagca gttcggcaac ggcttcgtcc tgccggcggg acctttgcgc
      601 gagttcgtga gtcaaggcct ggcgcgcgcc gatgcggtcg tcatcgtcgg cgacggcggc
      661 ccggcgctga ccaactatag cgggccgatt ctgcgtgccc acctgaccca tgtcgatacg
      721 ccggatctcg ccggccggcg cgtcgtcggt ttcgcgggga tcggaaggcc ggagaaattc
      781 ttccgctccc tgcgcgcctt cggcgccgaa atcgtcgcca ccaagcgctt cggcgaccat
      841 catatctata cgccgtcgga aatcgcccgc ttgaaggcga ccgcgcgcga ggcgggcgct
      901 ttgctcgtta ccaccgaaaa ggactatgtg cgcatgaccg aggtcgaacg cgaaggaatc
      961 acggttctgc cgatccgggc ggccatcgac aacggcaagg ccctggagct cctgcttgac
     1021 agactgtgcg cgtcgcgtta gcgcgccacc cggatgagca aacaagcccc cctgactttc
     1081 tggaaaaaac tgcgatacgg cgccgaagcg gcggcgtttt ttctcttgat ggggttcttc
     1141 aggctgatcg ggatcgacgc ggcatcggcc gccggaggct tcatcggccg gcacatcttc
     1201 tatcgcctgc ccacggccga tacggcgcgc gcgaatcttc gcgcggccta tccggacatg
     1261 gacgatgcgg aacgcgaaaa gaccgtgcgc gagatgtgcg acaatctcgg gcgcgtcgtc
     1321 ggggaatatc cgcatctgga caagttcacc ttgggtccgg gcgggcgtgt cgaagtgtcc
     1381 ggcatgaaga acgccgaagc ggccatcgcc aacggcaagg gcgtcatgtt catctccggc
     1441 catctcgcca attgggaagc gctgccgatt acaggcaatc ttctcggcta cgacggcgcg
     1501 ctggtctatc gtccgcccaa caatccgttc gtcgatcgct ggatttcgcg ccagcgcgcc
     1561 aagctgggcc ccaaggaaca catcagcaaa ggggcgcaag gaacgcggcg gatcttcacc
     1621 ctgctgcgca aaggcaaatc gatcctgatg ctggtcgatc agaagacctg ggaaggcgtg
     1681 cccgccccgt tcttcggccg cgacgcgatg acgacgccgg cgcccgcttc gctggcgctc
     1741 aagctggggt ctatcctgct gcccgcctcg gccgaacgca tcggcggcgc ccgtttccgc
     1801 gtgacgatcc atgagccgat cgaattcgag ccgagcggcg acgacaagcg cgatgtgctg
     1861 gcgctgaccg caaagatcaa ccaagccatc gagagcatgg tgcgggcgca cccttcgcaa
     1921 tggctctgga ttcatcaccg ctggcccggc ccgcgcgacg ccgtcaagat gaaaaacaaa
     1981 cagaacgccg tcagggctta ggcggtaccg gcggtaccgg cgagcgcgtg gaccgcgaag
     2041 gatcgagctt gacctgaaac caggcaagac cccagtgggt gtgtgggccg gtggcgcggc
     2101 cggtcttgcc gaccaagccg atgacatcgc cgcgcgcgac cttgtcgccg gccttcacca
     2161 tgcgttcgct ctgatgcaga tagcagctgg acacaccgtg gccgtgatcg agctgggtga
     2221 agccgccgtt gacgagaaag tcgccggtaa gcgacacgat ggcgtcggcc ggcgcgcgga
     2281 tcggcgttcc ttccggcgcg gcgatatcga cgcccagatg cggcgccatc ggcgtgccgt
     2341 tttcgatgcg gcggctgccg taaacgccgg tgacgatgcc gggaaacggc cagtcgaacg
     2401 gcccggcaaa accggtaccg ccactgtcgc gcgtgcgggc ctcggtgatc aaggcgttct
     2461 cgcgcttgat gcgttcgaga ttttccggcg atggcgtgac gagttcctgc ggcaggcctt
     2521 cgatgctctg agtttcgtat tggcggggga tgggcgccac gtcgcgcgct tcgctgccgc
     2581 cgtccgcata aagcgcgacg acatgcgcgg gctcgatgcg atcataggaa aaaccgaaaa
     2641 cgaaattgcc ttgcggcgaa agccgtaccg ccgcgccatc gacgctgacc gccgctccgg
     2701 ccggcgcctt gccgatcacc agaccgccct gctccgtcga tccaccgaag gaaagcgcgt
     2761 tgacctcggc gcgcgccaag cgcggcaaga cgatcgtcgc cgcccccgcc gccaggaagg
     2821 tgcgccgcgc cagcctcaag gcgtctttcc cgtttcctga aagcgtttga gggccgccgc
     2881 cgccgtggcg taagcctctt gcagttcgac gttccaatag cgcagcgcat cgagcggaat
     2941 ctgcacgccc gtgaccgcgc agatgacgaa agaacccgct ttcaccacgc ggtattcgcc
     3001 gtcgagatat tccagttcgg ccagaccctc gggcctaact tcgcgttcca tcttgttcat
     3061 tttaactcgc ccggagacct ttcctgcaaa ccgatttcta cgcgaccgga acatccggcg
     3121 ccattaaaac agatcgcctt ggtcgccgcc cgcttttttg ccgccggatt tgggcttgcg
     3181 cccgccttcc ccagcgacag caggcgccac gccgtcaccg aaggttaaca atagacgctc
     3241 gccggggatc aaggcggcgg cgcggcgacg caaagtgccg tccgcaccgc ggaccaaggc
     3301 aaatccccgc gccagcaccg catggtggct gactccgtcg agcaggcgcg aaaggccctc
     3361 aaggcgggca gcaaattcgg ccagacgcgt gcgctgattg cgccccagcc gcaatgccag
     3421 cgcttgcgtt cgctcggcgc cgagcgaaag gcggttcttg aggcccgccg gccggagcaa
     3481 ggacgatacc gcgatgaagg cgcgccggtg ggcgccgagg ttgccgcgca aggcatggcc
     3541 gagacgttcg gccgccacat cgacgcgctg gcgcggctgg gcgaacagtt gctcggcacg
     3601 aggcaagacg cgggcaagtt cccgcaccgc ccggcggcga tcctccatgc tcctgacgaa
     3661 ggcgcgcagc aggcggcgct cgaaatccag cgtctgggcg atcagttcgg tgcgcaccgg
     3721 aaccgccagt tcggcggcgg cggtgggcgt gggggcgcgg atgtcggcgg cgaaatcaat
     3781 cagcgtggtg tcggtttcgt gccccaccgc cgagatcacg ggaatggagc actcggcgac
     3841 ggcgcgcacg acggcttctt cgttgaacgc catcaaatcc tcgatcgagc cgccgccgcg
     3901 cgccacgatc agcacatccg ggcgcggcag accggcctgc gggaattccg taaagccgtg
     3961 tatggccgcc gcgatctctc cggccgcgcg ctcaccctgc accgccaccg gccacagcag
     4021 cacgcggcgc ggaaagcgcg cctgaagccg gtgcatgatg tcgcggatca ccgcgcccgt
     4081 cggcgaagtg acgacgccga tcacttcggg caggaacggc agcttctttt tgcgcgaggc
     4141 ctcgaacaag ccttcggcgg cgagtttctt cttacgctct tccagcatcg ccatcagcgc
     4201 gcccaggccc gccaactcca cctgttcgac gatgagctga tagcgcgaag aaccggcata
     4261 ggtggaaacc cgcccggtgc agacgacttc cagtcccgct tccgggcgga ccttcaagct
     4321 acgggcggtg cccttccaga tcacggcatt cagcaccgcc ttgtcgtcct tgaggtcgaa
     4381 atagacatgg ccggaggaat ggaatttcag gccgctgatc tcgccgcgca cgcgcacata
     4441 gggaaaagcg tcctcgaccg tgcgcttcaa ggcggccgag agttcggtaa cactgagttc
     4501 gggcaaattg gctagatcgg acatgattca cagatgatat aggagttgcg cttgaataca
     4561 ccctcccctt gagggagggt cgaaatctgc gaagcggatt tttccgcgct cggcctacga
     4621 aagctccact ggagctttcg tctacggctt cgaccctcaa ggggggaggc aaggagtgtc
     4681 gttgtgaacg ttctcctcat cggttccggc ggccgcgagc atgccctggc ctggtcgctt
     4741 gcggcgagcc ccatcgtctc gaaattatac tgcgcgccg
//
LOCUS       DASWPU010000075         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_55706,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000075 DASWPU010000000
VERSION     DASWPU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55706"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="VGC27_01645"
     CDS             complement(<1..1361)
                     /locus_tag="VGC27_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY0281308.1"
                     /translation="MNDNPEDRPAPDGGIPEAPAQPHETPQERQPWELSGPPDGFTRS
                     AAGPPPDDFEPPGEPPEWRQQTLQTKQEFLTWWRSWRPLRARAVLYGGLSAGALLIVG
                     ALAVAIYVWSVTRGLPSVEDLQNYAPAITTRVYAGDGTVLGEYARERRVFVPISFVPK
                     LVINAFTSAEDRNFYNHPGVDPSSILRAALKDIGKVLAGRRPEGASTITQQVAGGFML
                     DRSQVKIKRKIQEAVLALRLESTYSKEKILELYLNQINLGQNSYGVAAAALNYFGKSL
                     DQLSISEVAFLAAVPKAPSHYDPRFHLQAATDRRNWVIRQMEENGYITREQAAAAAAE
                     PLIANTRPLGSQTEDANYFVEEVRRQLYAKYGEHALYDGGLQVRSSLDTRLQNYAVNA
                     LRMGLVRYDRRHGWRGALSNIDVGGDWQTTLKSIGNRSGIGSWRVAAVLGFSPDKSVR
                     IGLS"
     gene            complement(1395..>1986)
                     /locus_tag="VGC27_01650"
     CDS             complement(1395..>1986)
                     /locus_tag="VGC27_01650"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0281309.1"
                     /translation="QAHGYTVYMTRDSDVFIPLRQRVKMARSWRADLFISIHADSIHD
                     PSVDGLSIYTLSDKGSDKEAAALAAKENQSDVIAGVDLSGENSAVAPILIDLAQRDTM
                     NKSSRFAETAIAELSHATDILPRQPHRSAAFVVLKAPDVPAVLIELGYLSNPRDAEQM
                     NTAAWRSGVADAIARAVDRHFATATADELKAAQRIQ"
BASE COUNT          347 a          629 c          632 g          378 t
ORIGIN      
        1 gaaagcccga tgcgcacgct cttgtccggc gaaaagccca gcaccgccgc gacgcgccag
       61 ctcccgatgc ccgaccggtt gccgatgctc ttcagcgtcg tctgccaatc gccgccaacg
      121 tcgatgttgc tcagcgcgcc gcgccagccg tgccgccggt cgtaacgcac cagccccata
      181 cgcaaggcgt tgaccgcgta attctgcagc ctggtgtcga gcgaagaacg cacctgcaag
      241 ccgccgtcat agagcgcatg ctcgccgtat ttggcgtaga gctggcggcg cacttcttcc
      301 acgaaatagt tcgcgtcttc cgtctggctg ccgagcgggc gcgtgttggc gatcaaaggt
      361 tcggccgccg cggccgcggc ctgctctcgg gtaatatagc cgttttcttc catctgcctg
      421 atgacccaat tgcggcggtc ggtggcggct tgaaggtgaa agcgcggatc gtaatggctc
      481 ggggctttcg gcaccgcagc caggaacgcc acttccgaga tcgaaagctg gtcgagcgac
      541 ttgccgaaat agttcaacgc cgccgccgcg acgccgtagg agttctggcc gagattgatc
      601 tggttgaggt acagttcgag gattttctcc ttcgagtagg tggattccag gcgcagcgcc
      661 aggaccgctt cctggatctt gcgcttgatc ttcacctggg agcggtcaag catgaagccg
      721 ccggccactt gctgggtgat ggtggaagct ccttccggcc ggcgtccggc cagcaccttg
      781 ccgatatcct tgagcgccgc gcgcaggatg ctcgagggat cgacgccggg gtggttgtag
      841 aagttgcggt cctcggccga ggtgaaggcg ttgatcacca gtttgggcac gaaggagatg
      901 ggcacgaaga cgcgccgctc gcgggcgtat tcgcccagca ccgtgccgtc gccggcatag
      961 acccgcgtgg tgatcgccgg cgcgtaattc tgcaagtctt ccacgctcgg cagaccgcgc
     1021 gtcaccgacc agacgtaaat cgccaccgcc agcgcgccga cgatcagcag agctcccgcg
     1081 gacaatccgc catatagaac cgctctggcg cgcaacggtc tccaggagcg ccaccacgtc
     1141 agaaattcct gcttggtctg caaagtctgc tgccgccact ccggcggttc gccgggcggc
     1201 tcaaaatcgt cgggcggcgg cccggcagcg gaacgggtaa atccatccgg cggaccggaa
     1261 agttcccaag gctgacgttc ctgcggcgtt tcgtgcggct gcgcaggtgc ctccggaatg
     1321 ccgccgtccg gcgcgggtcg atcttctgga ttgtcgttca tggggcgcga ttatgaccgt
     1381 gctaagccgt tccgtcactg tatgcgttgc gccgctttca gctcatccgc cgttgcggtt
     1441 gcgaaatgac ggtccacagc tcttgcgata gcatcggcga cgccactccg ccaagcggcc
     1501 gtattcatct gttcggcatc gcgcgggttc gacaggtaac ctagctcgat cagcaccgcg
     1561 ggcacatccg gagccttcag caccacgaac gcggcggaac ggtgcggctg gcgtggcaaa
     1621 atgtccgtgg cgtgcgaaag ttctgcaatg gcggtttcgg cgaaacgtga agatttgttc
     1681 atcgtgtcgc gctgcgccag gtcgatgagg atcggggcca ccgcggaatt ttcgcccgac
     1741 agatcgacac cggcgatgac gtcggactgg ttctccttcg ccgccagagc cgccgcctcc
     1801 ttgtccgatc ccttgtccga cagcgtatag atcgaaagac cgtccacgct tgggtcgtga
     1861 atggaatccg cgtggatcga aatgaacagg tcggcgcgcc aggagcgagc catcttcacc
     1921 cgctgccgca aaggaatgaa gacgtcgctg tcgcgcgtca tgtacacggt atagccgtgc
     1981 gcctgg
//
LOCUS       DASWPU010000076         4287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_86121,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000076 DASWPU010000000
VERSION     DASWPU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86121"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VGC27_01655"
     CDS             <1..702
                     /locus_tag="VGC27_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0281310.1"
                     /translation="QDELNHVARVTEMGQFSAALAHELNQPLAAIMNYMNVAKRLLAM
                     PGSEAKTQEALAKAGEQAQRAGQIIRRLRDFVEKRDGKRTAENINAIAEDAIALGLVG
                     AKAADIKVRTAFAPHSPPVLVDRVQIQQVLVNLLRNASEAMAGLPRRELTITTADIDG
                     KAVDVAVADTGPGIPDEVAKQLFKPFVTTKPGGMGIGLAISQSIIEAHGGHLRMTPNS
                     GGGTVFRFRLPAAAE"
     gene            706..1314
                     /gene="fixJ"
                     /locus_tag="VGC27_01660"
     CDS             706..1314
                     /gene="fixJ"
                     /locus_tag="VGC27_01660"
                     /inference="COORDINATES: protein motif:HMM:NF006900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HEY0281311.1"
                     /translation="MAQGGKVFVVDDDPGIRDSLAMLLTASGYEAKPYASAAAFLASE
                     TPDAAGCMVVDVQMPEMNGLVLQQELAARHFRLPVVIMTGHGDIPIAVQAMKAGAVDF
                     LEKPFDEEVLLGCIRRALESAPPAEDQGPAVAETKRRLEQLTDRERQVLDLIVAGKAN
                     KVVAYELSISPRTVEIHRSRVMEKMGAGNLADLVRKVLSLSS"
     gene            1397..1762
                     /locus_tag="VGC27_01665"
     CDS             1397..1762
                     /locus_tag="VGC27_01665"
                     /inference="COORDINATES: protein motif:HMM:NF018228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0281312.1"
                     /translation="MPLIVDIVEDDDAVRDSICALLEAYGHTARGYASAELYLNRAAA
                     PVHCLIVDLHMPEMSGLELLETLRSQDVRVPALIMTGRHDPSLEPRMARAGVVKLLQK
                     PIQDEQLFGWIDQACAAPH"
     gene            2094..2309
                     /locus_tag="VGC27_01670"
     CDS             2094..2309
                     /locus_tag="VGC27_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281313.1"
                     /translation="MSMTESPRGNPLSPALSAAATNPFLRMKTAAQLAAYREAVLAYL
                     RQIKADLAGDPHAAEVIYRVVNSTITA"
     gene            2434..2889
                     /locus_tag="VGC27_01675"
     CDS             2434..2889
                     /locus_tag="VGC27_01675"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY0281314.1"
                     /translation="MFSRFVTTNNLHLAAIVALVFLILVVPAKAAAIVGNGWMRLLPA
                     GLPAAGYFELHNDGDKTLSLTGASSPACGMLMLHKSENVGGTMRMEEVSSVDVAPRAT
                     LKFAPGSYHLMCMNPSPALKPGAHVAVTLAFSGGAKVTSEFAVRNAAGK"
     gene            complement(2927..3835)
                     /locus_tag="VGC27_01680"
     CDS             complement(2927..3835)
                     /locus_tag="VGC27_01680"
                     /inference="COORDINATES: protein motif:HMM:NF011991.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEY0281315.1"
                     /translation="MSKAPPRLELTLGPVLFNWPVQRWVDFYARIADEAPVDRVCLGE
                     VVCSKRQPFKDEVILDTVDRLERGGKEVVYSTLALPTTKREVRAIGEMIEAGYLIEAN
                     DIATVQLLNGRHHVIGPFLNIYNEAALALHLKLGATRVCLPPELPLESVRLIAAGNGA
                     VEVFAFGRAPLALSARCYHARAHGVAKDACQFVCERDADGLDVLTLDGKPFLAVNGVQ
                     TLGYVVTAVTREIADLRAAGVVSLRLSPQSCDMVKVATIFRALADGHTTQEEATEALG
                     VIALPGPLSDGFLRGLPGARLLDEVD"
     gene            complement(3832..>4287)
                     /locus_tag="VGC27_01685"
     CDS             complement(3832..>4287)
                     /locus_tag="VGC27_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEY0281316.1"
                     /translation="RCMLSSYAIGQSPNMTGVCSPPNHVDFREDNGAMIASVGGFTVE
                     RFAQGEAAGYPTLCKGRFQAGGGFLHLFEDPVSLNAVELLPRLADIGVAAFKIEGRQR
                     GRAYVAAVVRAFRRAIDSMAAGGKIDVSELRALTEGQRDTTGSYKKAWR"
BASE COUNT          792 a         1435 c         1362 g          698 t
ORIGIN      
        1 caggacgagc tgaatcacgt cgcgcgcgtc accgagatgg gccagttctc cgccgccctg
       61 gcgcacgaac tcaaccagcc gctcgccgcc atcatgaact acatgaacgt ggccaagcgg
      121 ctgctcgcca tgcccggcag cgaggccaag acgcaggagg ccctcgccaa ggccggcgaa
      181 caggcgcagc gcgccggaca gatcatccgc aggctgcgcg acttcgtcga gaagcgcgac
      241 ggcaaacgca ccgccgagaa catcaacgcc atcgccgagg acgccatcgc gctcgggctg
      301 gtcggcgcca aggccgcgga catcaaggtg cgtaccgcgt tcgcgcccca ttcgccgccg
      361 gtgctggtgg acagggtgca aatccagcaa gtcctggtca atctgctgcg caacgccagc
      421 gaagcgatgg caggcttgcc cagacgcgaa ctgacgatca ccaccgccga catcgacggc
      481 aaggccgtgg acgtcgccgt tgccgatacc gggccgggca tccccgacga ggtggcgaaa
      541 caactgttca agcctttcgt caccaccaag ccgggcggca tgggcatcgg actggccatc
      601 agccaatcca tcatcgaagc ccatggcgga catttgcgga tgacgcccaa cagcggcggc
      661 ggcacggtct tccgcttccg cctgccggcg gcagcggagt aggcgatggc acaaggcggc
      721 aaagtcttcg tcgtcgacga cgatcccggc attcgcgatt ccctcgccat gctgctgacg
      781 gcatccggct acgaggcgaa accctacgcc tcggcggccg ccttcctggc gagcgagacg
      841 ccggacgcgg ccggctgcat ggtcgtggac gtgcagatgc cggagatgaa cgggctggtg
      901 ttgcagcagg aactcgcggc gcgccacttc cggcttcccg tcgtcatcat gaccggtcac
      961 ggcgacattc cgatcgcggt gcaggccatg aaggcgggcg ccgtcgattt tctggagaag
     1021 ccgttcgacg aagaggtgct gctcggctgc atccggcgcg cgctcgaaag cgcgccgccg
     1081 gccgaagacc aggggcccgc cgtcgcggaa accaagcggc ggctggagca gttgacggac
     1141 cgcgaacggc aggtgcttga cctgatcgtg gctggcaagg ccaacaaggt cgtcgcctac
     1201 gaattgtcga tctcgccgcg cacggtggaa atccaccgct cccgcgtgat ggagaagatg
     1261 ggcgccggca atctggcgga tctcgtgcgc aaggtgctgt ccctatcttc ttaggggata
     1321 ttacgtattt cgcctcctgc cgggcgcggc tagcctcaaa atcaccttaa tcgtacagcc
     1381 agcggagatt ggcgacttgc ccctcatcgt ggacatcgtc gaagacgacg atgcggtgcg
     1441 cgactccatt tgcgcactgc tggaagcgta cggccatacg gcccgcggct acgcttcggc
     1501 ggagctttac ctgaaccgcg ccgccgcgcc cgtccattgc ctcatcgtcg atcttcatat
     1561 gccggaaatg tcggggctgg agcttctcga aaccctgcgc tcgcaagacg tccgcgttcc
     1621 ggcgctgatc atgaccggcc gccacgatcc gtcgctggag ccgcgcatgg cgcgggcggg
     1681 cgtcgtcaag ctgctgcaaa agccgatcca ggacgaacag ctcttcggct ggatcgacca
     1741 agcctgcgcc gcgccgcact aagcgtgatc caatcaaatt gagtcacgac agtcaccctt
     1801 cgacaagctc agggtgaggg ccaattgaaa tcccttcatg cgtagcctgc ccgccttcgc
     1861 tgcgctacgg tggaccaaag ctcgatggcc cgcccgccgg agctttagcg caggcgggtc
     1921 gaagcatgag aggggcaaga tcccattcgg atggatcgcg tactaacggc gccgcatcgt
     1981 ggaaaaccct taggggaaat ccacgactac aaaaccctcc cgcttcttgc caatactctt
     2041 cccggcaaca gaatggtggc ggcgaactca tgttggcatc agaacccagc gtcatgagca
     2101 tgaccgaaag tcctcgtggc aaccccctca gcccggctct ttcggccgcc gccaccaacc
     2161 cctttctgcg gatgaagacg gcagcccagc ttgctgcata tcgcgaggcg gttctggcgt
     2221 acctgcgtca gatcaaagcc gatctggccg gcgatcctca cgccgccgaa gtgatttacc
     2281 gggtcgtgaa ttctacgata accgcttgat ggaactgatg aaatttctct aatgtcgggc
     2341 cgtccgtaag tgcgacatag aattacttga ccttagccat gctctcgcag actttcgccg
     2401 gtatgccaac ccgaaacttc gcgcgagacg tccatgttct cccgtttcgt gaccaccaac
     2461 aatcttcacc tcgccgctat cgtggcgctg gtcttcctga ttctcgtggt cccggccaag
     2521 gccgccgcca ttgtcggcaa cggctggatg cgcctgttgc cggctggcct gcccgcggcg
     2581 ggctatttcg agctgcacaa tgacggcgac aaaacccttt ccctgaccgg cgccagttcg
     2641 cccgcatgcg gcatgctgat gctgcacaag agcgaaaatg tcggcggaac gatgcgtatg
     2701 gaagaggttt ccagcgtcga cgtggcgccg cgcgcgacgc tgaaattcgc gccgggctcc
     2761 tatcatctga tgtgcatgaa cccgagcccc gcgctgaagc cgggcgcgca tgtggccgtc
     2821 accctcgcct tctccggcgg cgcgaaggtg acgagcgaat tcgcggtccg caacgcggcc
     2881 ggaaaataac gttccgccga acacgcgccg aaacggcgcg gaccgctcag tccacctcgt
     2941 ccagcaggcg cgcgccgggc aggccgcgca ggaagccgtc ggacagcggt ccgggaagcg
     3001 caatgacgcc caaggcttcg gtggcttctt cctgcgtggt gtgaccgtcg gccagagcgc
     3061 ggaagatcgt cgccaccttg accatgtcgc agctttgcgg cgacaggcgc agcgagacga
     3121 caccggccgc gcgcaggtcg gcgatctcgc gggtcaccgc cgtgaccacg tagcccaggg
     3181 tctggacgcc gttgaccgca agaaacggct tgccgtcgag agtcagcacg tcgaggccgt
     3241 cggcgtcgcg ctcgcagacg aactggcagg cgtccttcgc cacgccgtgc gcgcgcgcgt
     3301 ggtaacagcg cgccgaaagc gccagcggcg cgcggccgaa ggcgaacact tccacggcgc
     3361 cgttgccggc cgcgatcagg cggaccgatt cgagcggaag ctccggcggc aggcaaaccc
     3421 gcgtcgcgcc cagcttcagg tgcagcgcca gcgccgcctc gttgtagatg ttgaggaacg
     3481 ggccgatcac gtgatggcgg ccgttcaaga gctggacggt ggcgatgtcg ttggcctcga
     3541 tcagatagcc cgcctcgatc atctcgccga tggccctgac ctcgcgcttg gtcgtcggca
     3601 gcgccagggt cgaatagacc acttccttgc cgccgcgttc gagccggtcc accgtgtcga
     3661 gaatgacttc gtccttgaac ggctggcgct tcgaacacac gacctcgccg agacagacgc
     3721 gatcgaccgg cgcctcgtcg gcgatgcggg cgtagaaatc gacccagcgc tggaccggcc
     3781 agttgaacag caccggcccc agcgtgagtt ccagacgcgg cggtgctttg ctcatcgcca
     3841 cgccttcttg taggagccgg tggtatcgcg ctggccctcg gtcaaggcgc gcagttcgct
     3901 gacgtcgatc ttgccgccgg cggccatgga atcgatggcg cggcggaagg cgcgcaccac
     3961 cgccgcgaca taggcgcggc cgcgctgacg gccttcgatc ttgaacgcgg cgacgccgat
     4021 atccgccagc cgcggcagaa gctcgaccgc gttgaggctg acgggatcct cgaacagatg
     4081 caggaagccg ccgcccgcct ggaagcgccc cttgcacaag gtcggatagc cggccgcctc
     4141 gccttgcgca aaccgttcga cggtgaaccc gccgacggag gcgatcatcg cgccgttgtc
     4201 ctcgcggaaa tccacgtggt tcggcggcga acaaacgccg gtcatattgg gcgactggcc
     4261 gatggcgtag gacgacagca tgcagcg
//
LOCUS       DASWPU010000077         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_74006,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000077 DASWPU010000000
VERSION     DASWPU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74006"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VGC27_01690"
     CDS             <1..404
                     /locus_tag="VGC27_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0281317.1"
                     /translation="RDVGMDPATRFRYPHEFSGGQRQRISIARAVVLESSFVVLDEPT
                     SALDMLFQAQMVDLLRELQRKRDLTYMFISHDLRVVASLASHLIVMRQGKVVEEGPAS
                     ELFKHPKTDYTRALFAAAFRIEAAPGGAVAE"
     gene            complement(453..1706)
                     /locus_tag="VGC27_01695"
     CDS             complement(453..1706)
                     /locus_tag="VGC27_01695"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY0281318.1"
                     /translation="MSKRLVAHGGKGISVRQLGGNRAGEMRITRFLHNPKVTLSEMVS
                     AALARTCAQVAGRHVLAIQDTSALRVDEKGVGLSFHPVIAVDANAGTVLGLVDNVFLT
                     RRGGERAKRKQRDFTEKDSRRWLNGAERASALVAAGAACVTVVEDREGDIYECFAFKP
                     ANVEKLVRAAQDRHLADGTSLFSKADVWDEAGRMVVGLAAAPGRKARSAELSVKFGTV
                     EIVRPTNRKASCGLPKTKAVTLVIGREINPPDGGEPALWFLLTTHQVNDIADARRIIG
                     FYRLRWTIEQLFRTMKTKGFDVEALRQQQDGPLEKLVTAILIAAVKVMQLVDEREGRA
                     KRPLSDVFDPDDQPALEQVCQSLEGKTAKQTNPHPVGSLAYAAWVFARLGGWTGYYGK
                     PGPIVMLRGLTQFHAIKHGWTLRYV"
BASE COUNT          385 a          692 c          666 g          403 t
ORIGIN      
        1 tgcgcgatgt cgggatggat ccggcgaccc ggtttcgcta tccgcatgaa ttctccggcg
       61 gtcagcgcca gcgcatcagc atcgcccggg cggtggtgct ggaatcgagt ttcgtggtgc
      121 tggatgagcc gaccagcgcg ctcgacatgc tgttccaggc gcagatggtc gacctgttgc
      181 gcgaactgca gcgcaagcgc gacctgacct acatgttcat ctcgcacgat ctgcgtgtgg
      241 tggcctcgct ggcgagccat ttgatcgtga tgcggcaggg caaggtggtg gaggagggcc
      301 cggcttcgga attgttcaag catccgaaga ccgactacac ccgtgccctg ttcgccgccg
      361 cgttccggat cgaggccgcg ccgggcggcg ccgtggcgga gtagctggct gccgcggact
      421 ccgttttcac ctctcccgat gggctacggg attcacacat atcggagggt ccatccgtgc
      481 ttgatggcgt ggaattgggt gaggcctctg agcatgacga tggggcccgg ctttccgtaa
      541 tagccggtcc agccgcccag ccttgcaaag acccacgcgg cataggcgag cgagccgaca
      601 gggtgtgggt tcgtctgctt tgcggtttta ccttcgagac tttgacagac ctgttcgagg
      661 gccggctggt cgtcgggatc gaagacgtcg ctgagcggac gtttggctct gccctcgcgc
      721 tcgtcgacga gttgcatcac cttgacggca gcgatcaaaa tggcggtgac gagtttttcg
      781 aggggaccgt cctgctgctg tcgcagcgct tcgacatcaa aaccctttgt cttcatggtc
      841 cggaacaatt gctcgatggt ccagcgcaaa cgatagaagc cgatgatgcg gcgggcatcg
      901 gcaatatcgt tcacctgatg ggtggtcagc aggaaccaca gcgccggttc cccgccgtcc
      961 gggggattga tctcgcgccc gataacgagg gtcacagcct tggtcttggg caagccacag
     1021 gaggccttgc ggttggtggg acgcacaatc tcgaccgtgc cgaatttgac ggaaagctcg
     1081 gcgctgcgcg cctttcgacc cggggcggcg gcaagcccga caaccattct gccggcttca
     1141 tcccagacat cggccttgga gaaaagcgac gtgccgtcgg ccagatggcg atcctgagcc
     1201 gcccgtacca gcttttccac attggccggc ttgaacgcaa agcattcgta gatgtcgcct
     1261 tcgcgatctt ccaccaccgt gacgcaggcc gctccggcag cgaccaaggc gctggcgcgt
     1321 tccgccccgt tcaaccagcg tcgactgtcc ttctccgtaa agtctcgctg cttgcgcttg
     1381 gcgcgttcgc cgccccggcg cgtcagaaag acattatcga ccagtccgag caccgtgcca
     1441 gcgttagcat ccaccgctat gaccgggtga aacgacagcc ccaccccctt ctcgtccacc
     1501 cgcagcgccg acgtgtcttg aatggcgagc acgtgacggc cggcaacctg ggcgcaggtg
     1561 cgagccagtg ccgcagaaac catctcgctc agcgtcacct tgggattgtg cagaaaccgc
     1621 gtgatccgca tctcgccggc gcggttgcca cccaattgcc tgacactgat gcccttgccg
     1681 ccatgcgcga caagccgctt ggacaaaaag accccccttt tgccagacgt gcgtctccaa
     1741 actcccccaa cagcgcagcc accatgccct cccaaacacg aatcaccgct attaaaggag
     1801 tcagcttccg agcctcctgg caagcttcac aatgtgtgaa tgccgtagcc cggagggaga
     1861 ggtcggatcg cgctagcgat ccgggtgagg ggttacgctc tatcgataga cctgaatccc
     1921 tcacccggcg ctccgcgccg acctctcccc atcggggaga ggtgttctcc gcggatagac
     1981 cgagtaagcc aaagggaagc tcatgagcga gatggccgcc tccgtcgcac cgctcgagcc
     2041 gatcgaggac cggggcggcg cggcacagcc gctgctgcag gtcagcggcc tgaccaagca
     2101 tttcccggtg cgcggcgatc tcttcagcaa gcgccggacg gtgcgc
//
LOCUS       DASWPU010000078         3460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_86125,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000078 DASWPU010000000
VERSION     DASWPU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86125"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGC27_01700"
     CDS             <1..312
                     /locus_tag="VGC27_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281319.1"
                     /translation="IETRVVRISKVLDERLAALAAKTGRRKSYYASKAIAEYLEDRED
                     YLLAVAAYEESKGRRRFTLDEVEKRLGLETGNYSARGKGAKKARRGRSTAGEKVPQNQ
                     D"
     gene            716..800
                     /locus_tag="VGC27_01705"
     tRNA            716..800
                     /locus_tag="VGC27_01705"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:750..752,aa:Tyr,seq:gta)
     gene            929..1002
                     /locus_tag="VGC27_01710"
     tRNA            929..1002
                     /locus_tag="VGC27_01710"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:961..963,aa:Gly,seq:tcc)
     gene            1087..2283
                     /gene="tuf"
                     /locus_tag="VGC27_01715"
     CDS             1087..2283
                     /gene="tuf"
                     /locus_tag="VGC27_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY0281320.1"
                     /translation="MGKAKFERTKPHCNIGTIGHVDHGKTTLTAAITKILAETGKAQF
                     TSYDQIDKAPEEKARGITINTAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     IIVVSAADGPMPQTREHILLARQVGVPALVVYMNKVDMVDDPELLDLVEMEVRELLSS
                     YQFPGDKIPVIRGSALVALEGKDDPNHIGRDSVLELMKAVDDYIPQPNRPVDQPFLMP
                     IEDVFSISGRGTVVTGRVERGVVKVGETVEIIGIRATTSTTVTGVEMFRKLLDQGQAG
                     DNIGALLRGIERTGVERGQVLAKPGSITPHTNFKAEVYILTKDEGGRHTPFFTNYRPQ
                     FYFRTTDVTGIVQLPEGTEMVMPGDNVSIEVELIVPIAMEEKLRFAIREGGRTVGSGV
                     VAKIIK"
     gene            2330..2638
                     /gene="rpsJ"
                     /locus_tag="VGC27_01720"
     CDS             2330..2638
                     /gene="rpsJ"
                     /locus_tag="VGC27_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY0281321.1"
                     /translation="MQGQNIRIRLKAFDHRILDNSTREIVNTAKRTGAQVRGPIPLPT
                     GIERFTVNRSPHIDKKSREQFEIRTHKRLLDIIDPTPQTVDALMKLDLAAGVDVEIKL
                     "
     gene            2651..3382
                     /gene="rplC"
                     /locus_tag="VGC27_01725"
     CDS             2651..3382
                     /gene="rplC"
                     /locus_tag="VGC27_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY0281322.1"
                     /translation="MRTGVITQKVGMTRLFLEDGKHVPVTVLKLDGCQVVATRSDEKD
                     GYTAVQLGSGYAKTKRLTKSERGHFAKAEVEPKKKLAEFRVDAANLLEVGDVIQADHF
                     VAGQKVDVTGVTIGRGFTGAMKRWNFRGLEATHGVSISHRSLGGTGGRQDPGKTFKNK
                     KMHGHYGVDRVTTQNLEIAKVDVTRGLIMIRGAVPGSKGGWVMVRDAVKYPLPKEAPV
                     PASVKKAGKNNGDAAAPAEKAEGLT"
BASE COUNT          761 a          946 c         1145 g          608 t
ORIGIN      
        1 atagaaacac gcgttgttcg gatttcaaag gttctcgatg agcggctcgc tgctttggcg
       61 gccaagaccg gcaggcggaa aagctattat gcttccaagg ccatagcgga ataccttgaa
      121 gaccgggaag actatctgct cgctgtagca gcctatgaag agagcaaggg aaggcggaga
      181 tttacgcttg acgaagtcga aaaacggctc gggctggaga ctggaaatta ctccgcgcgc
      241 ggaaaaggag ctaagaaggc tcggcgcggt cgatcaacag cgggtgagaa agttcctcag
      301 aaccaggatt gaaggcacgg acaacccccg ccaaagcggc aaacctcttg gcggcataaa
      361 atcagaatat tggcgctatc gtgttggtga tattcgcatt ttatgtcgct tggaggacga
      421 acgggtagtc gtgatcgtgg ttgcgctagg ccaccgccgc gaggtctacc gctagccacc
      481 cggcttgctt tttcccaggc gccgggtcta gcctttggcc ggtcgctgcc gcgggggcag
      541 ttatccggct ttcccgcgtt agtggtctat tgacacactt aaacaattga cgaataaccc
      601 gcggtccgca gcccaatctc ggaaaggcac tattaccgtt cgtgtgattc acggacgttc
      661 ctcgtgcgcc gacggtggct ggcgcgtaag tggttgtgcc agttttgttt ttaccggagg
      721 ggtgcccgag tggctaaagg ggacggactg taaatccgtt ggcaacgcct acgttggttc
      781 aaatccaacc ccctccacca acctacgctg cggagcagcg aaggttgccc tccgaagccc
      841 gacagggcgc aggagggctg gaatcaggac cacgcagctt cggttggcac gccagccgat
      901 gaggcgaagg aaaaaggagt cgaatcaggc gggtgtagct caatggtaga gcaacagcct
      961 tccaagctga tgacgagggt tcgattccct tcacccgctc caacctgcgc cgcttcgcgg
     1021 cttcggttga caggccaaaa gacaaatacc gcgcgagcgg attgagaaga gacaaaggat
     1081 acgaccatgg gcaaagccaa atttgagcgg acgaagccgc actgcaacat agggacgatt
     1141 ggccatgttg accatggcaa gacgacgctg acggctgcga tcacgaagat attggcggag
     1201 actgggaagg cgcagtttac gtcgtacgac cagatcgaca aggcgccgga agagaaggcg
     1261 cgcgggatca cgatcaacac ggcgcatgtc gagtacgaga cggacaagcg ccattacgcg
     1321 catgtcgact gccccggcca cgccgactat gtgaagaaca tgatcacggg cgcggcgcag
     1381 atggacggcg cgatcatcgt ggtgtcggcg gcggacggcc cgatgccgca gacgcgcgag
     1441 cacatcctgc tggcgcgcca ggtgggcgtt ccggcgctgg tcgtatacat gaacaaggtg
     1501 gacatggttg acgatccgga gttgctggat ctggtggaga tggaggtccg cgaacttctg
     1561 tcgagctatc agtttcccgg cgacaagatc ccggtgatcc gcggctcggc gctggtggcg
     1621 ctggaaggca aggacgaccc caaccacatc ggccgtgact cggtcctgga gctgatgaag
     1681 gcggttgacg attacattcc gcagccgaac cgtccggttg accagccgtt cctgatgccg
     1741 atcgaggacg tgttctcgat ctccggtcgc gggacggttg tgacggggcg ggtggagcgc
     1801 ggggtggtga aggttggcga gacggtggag atcatcggca ttcgcgcgac gacgtcgacg
     1861 acggtgacgg gggttgagat gttccgcaag ctgctggacc aggggcaggc gggcgacaac
     1921 atcggggcgc tgctgcgcgg gatcgagcgg acgggggttg agcgcggcca ggttctggcg
     1981 aagccgggct cgatcacgcc gcacacgaac ttcaaggcgg aagtgtacat cctgacgaag
     2041 gacgagggcg gacggcatac gccgttcttc accaactatc gtccgcaatt ttacttccgg
     2101 acgacggacg tgacggggat cgtgcagctt cccgagggaa cggagatggt gatgcctggc
     2161 gacaacgtgt cgattgaggt tgagctgatc gtgccgattg cgatggaaga gaagctgcgc
     2221 ttcgccatcc gcgaaggcgg ccgtaccgtc ggctcaggcg tcgtcgctaa aattattaag
     2281 tagggaccgg ggaaatcggc ggtccgagag gccgtttcgg agaagtgcaa tgcaaggtca
     2341 aaatatccgc atccggctga aggccttcga tcaccgcatc ctcgataatt cgacgcggga
     2401 gatcgtcaac acggcgaaac gcacgggcgc gcaggtgcgc ggtccgattc cgctgcccac
     2461 gggcatcgag cgtttcaccg tgaaccgttc cccgcatatc gacaaaaaga gccgtgagca
     2521 gttcgaaatt cggacgcaca aacgcctcct cgacatcatc gaccccacgc cgcaaaccgt
     2581 cgacgctttg atgaagctcg accttgcggc cggtgtcgac gtcgagatca agctttaagg
     2641 agtaagcgcg atgcgcacag gcgtcatcac gcagaaggtc ggcatgaccc gcctcttcct
     2701 ggaggacggg aagcatgtgc ccgttacggt tctgaagctg gatggctgtc aggtcgtggc
     2761 gacgcgcagc gacgagaagg acggctatac ggcggttcag ctcggttcgg gttatgccaa
     2821 gaccaagcgt ctgaccaaat ccgagcgcgg ccattttgcc aaggcggaag tcgagcccaa
     2881 gaagaaactc gccgagttcc gcgtggatgc ggcgaacctt ctggaagtcg gggacgtaat
     2941 ccaggccgac catttcgtgg ccgggcagaa ggtggacgtc accggcgtca ccatcggccg
     3001 cggcttcacc ggcgcgatga agcgctggaa cttccgcggc ttggaagcca cgcacggcgt
     3061 gtcgatttcg caccgctcgc tgggcggcac gggcggccgt caggatccgg gcaagacctt
     3121 caagaacaag aagatgcacg gccattacgg tgtggatcgc gttacgacgc agaaccttga
     3181 aattgccaag gtcgatgtga cccgcggcct gatcatgatc cgcggcgcgg tgccgggctc
     3241 gaaaggcggc tgggtgatgg tgcgcgatgc cgtcaaatat cctctgccga aagaagcgcc
     3301 tgtaccggcc tcggtgaaga aggccgggaa gaacaatggc gacgcggccg ctccggccga
     3361 gaaagcggag ggcttaacat gaaagccaag gtcctcaacc tggacaacaa ggcgagcggc
     3421 gagatcgatc tcagcgatgc gatctacggt cttgagccgc
//
LOCUS       DASWPU010000079         6446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_576, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000079 DASWPU010000000
VERSION     DASWPU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VGC27_01730"
     CDS             <1..201
                     /locus_tag="VGC27_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281323.1"
                     /translation="DGAVKAAQSEYDSLAADAGAPDSVRQRAVAMASLLRAGGVENYG
                     IVPPPAIPSDASPSDQKGNAPQ"
     gene            198..1607
                     /locus_tag="VGC27_01735"
     CDS             198..1607
                     /locus_tag="VGC27_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0281324.1"
                     /translation="MTSRPLRLVAAVAAAALLLSGCVVTDTVGKWFSSSGTKSKLKGQ
                     RISVMSLDESVKPDPALQDTPVILPAPYRNTEWPQPGGYAANAMYHLEASGPLRRVWK
                     QDAGAGSNSDSRLTAAPIVAGGKVYVLDARARVYAFDANSGKPAWHVSVAPKGHTDLI
                     NVLSFGLFGEDTKVDPSKGFGGGIAFDDGKLFATTGFGDVIALDAATGKELWRTNVGV
                     PIFNAPTANGGRVFVSSQDNHFHAFAQNNGRELWDHQGITEAAGILQSTSAAVAGEFV
                     IAPYTSGELYALRVQNGRPAWSDMLTRSGNVTALTELDDIAGRPVVDRDLVFAISHSG
                     VMAAIQLGTGDRQWTRDLASIQTPWVAGDFVYVLTTQAQLLCLTRKEGRVKWLHQLPR
                     WTDPKDTSSDPIVWSGPVLVSNRLIMVSSDGYAVSVSPYSGELLGRMEIPAGAYIAPV
                     VADDTLYILTNDADLVALR"
     gene            1698..3227
                     /gene="der"
                     /locus_tag="VGC27_01740"
     CDS             1698..3227
                     /gene="der"
                     /locus_tag="VGC27_01740"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY0281325.1"
                     /translation="MPLTIAIVGRPNVGKSTLFNKLAGRKLALVHDTPGVTRDRREAD
                     ARLGALSLRLFDTAGIAHGAKDSLAARMTAQTMTAIADADVCLFMIDGREGVTAADEI
                     VAEAVRRSGKPVVLAVNKCEGRQTHLGEGEGWALGFGEPVAISAEHRLGLEELVEALA
                     PFAKAEEPVEENPDGDKPLRLAIIGRPNVGKSSLFNYLLGSERALTGPEAGITRDAIA
                     ARWQAEGREILLHDTAGLRKTACAAGHTLEELSVASTLDAVRFADCVIVVIDQTAPFE
                     KQDLTIADMIAREGRAAVFAANKWDLAANRAGAIGRLREKLDRLLPQLAGAPLLAVSA
                     VTGEGIDRLLPAVLAADRAWNTQAAGAHGALGQICGHQRHRFRRGHACLRRARAGPRR
                     NLDQRRNPAPVHSAARQRPRPFARMPARRKAGGRALRRAAGRRLLRREHVLAPDRRQQ
                     SGAGPSGGAAQARRLQAARRPVPDRTSGAFRRGGNSARGVPCQTERSDFRPGLLAGVF
                     G"
     gene            complement(3204..3737)
                     /locus_tag="VGC27_01745"
     CDS             complement(3204..3737)
                     /locus_tag="VGC27_01745"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEY0281326.1"
                     /translation="MMLRSAVAALAVLAAAAEAQSPPDASMNIMLTPPPELRTGSAAR
                     PVLELRALDKITGRASVLLAPLNVPVKYATLTIVARTCYSTPASETPETSAFLQIEDH
                     RPDQPERRVFSGWMYASSPGLNGMQHPLYDVWAISCKTNAPDQAPLVTASTPVKPVSP
                     NAGANEALPELPEDAGQ"
     gene            complement(3734..4207)
                     /gene="mlaD"
                     /locus_tag="VGC27_01750"
     CDS             complement(3734..4207)
                     /gene="mlaD"
                     /locus_tag="VGC27_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY0281327.1"
                     /translation="MHNDLVETLIGAAVVAVAVIFLVFAYQSTGSASVKGYELTAKMA
                     RVDGIAVGTDVKLSGIKVGEVSSLTLDPKDYLVIVHLNVHNDVKIPDDSSLMVTSSGL
                     LGSSYLSIAPGGSDKVLAADGRIRNTQGSVDLMALLSRFAGGAASGSGNSEETRP"
     gene            complement(4214..4663)
                     /locus_tag="VGC27_01755"
     CDS             complement(4214..4663)
                     /locus_tag="VGC27_01755"
                     /inference="COORDINATES: protein motif:HMM:NF006040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEY0281328.1"
                     /translation="MVSATPLAKTPSRLYIPVNLIGAVMRFFWMLFAWWRGATYGTLL
                     TTWLKGVPVGTDAYGNRYYRTKDGKRRWVLYNGMVEASRVPADWHGWLHHTFKEPPGG
                     PALIKPWEKDHLPNMSGTKGAYYPPGSLARGGVRAPATGDYEPWKPV"
     gene            5160..5528
                     /locus_tag="VGC27_01760"
     CDS             5160..5528
                     /locus_tag="VGC27_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0281329.1"
                     /translation="MTEQLPFRPLIWVGSTRRIFRAMPAEVKSRMGYALYVAQLGGKH
                     RDARPLRGFGGAGVLEIVKDHRGDTFRSIYTVRLAGAVYVLHVFQKKSKTGRETPKTD
                     MNLIETRLREAERIAKDVRT"
     gene            5525..5860
                     /locus_tag="VGC27_01765"
     CDS             5525..5860
                     /locus_tag="VGC27_01765"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0281330.1"
                     /translation="MKKKTDYEMGTGNIFADVGLPNAEEHLVKAQLVYRIDGLMKERG
                     LKQIEAAKLFGVKQPDVSKMLRGDFRQFSVERLMRFLVALGQNVEIVVTPHRGRKETA
                     QLRVAPAVA"
     gene            5955..>6446
                     /locus_tag="VGC27_01770"
     CDS             5955..>6446
                     /locus_tag="VGC27_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281331.1"
                     /translation="MARMTDPSDALNSFQQALRHGEVRPQRCETDSNLFVHLDHPNGE
                     VRITYVRIYHETVTALASFILGAPIDGLPCFSIGYAVPEKFRNQGRAKDIIEAGIAEM
                     KYGLFSRANISSFYVEAVIGVDNKPSRHVAAATISFNPLPVTDHVSGLPAFQYVRKIE
                     NNTV"
BASE COUNT         1266 a         1991 c         2012 g         1177 t
ORIGIN      
        1 gacggcgcgg tgaaggccgc tcaaagcgaa tatgacagcc tggcggccga cgccggggcg
       61 cccgacagcg tgcgccagcg cgccgtcgcc atggcgtcgc tgctacgcgc gggcggcgtg
      121 gagaattatg ggatagtacc cccgcccgcc ataccaagcg acgcctcgcc aagcgatcag
      181 aaaggaaacg cgccacaatg acgtcgcgcc cgttacgcct cgtggcggcc gtcgccgccg
      241 cagcgctttt gcttagcggc tgcgttgtca ccgatacggt gggcaaatgg ttcagcagca
      301 gcggcaccaa atccaagctt aaaggccagc gtatctcggt catgtcgctc gacgaatcgg
      361 tcaagccgga cccggccctg caagacactc ccgtcatcct gccggcgcct taccgcaaca
      421 cggaatggcc gcagccgggg ggttacgcgg ccaacgccat gtatcatctg gaggcgtccg
      481 gaccgctccg gcgggtctgg aaacaagacg ccggcgcggg atcgaattcg gattcgcgct
      541 tgaccgcggc accgatcgtc gccggcggaa aggtttatgt cttggacgcc agggcgcgcg
      601 tctacgcctt cgacgcaaac agcggcaagc cggcctggca tgtcagcgtc gcgccgaagg
      661 gacacaccga cctgatcaac gtgctcagtt tcggcctgtt cggcgaagac accaaggtcg
      721 atccctccaa gggcttcggc ggcggtatcg ccttcgacga cggcaagctg ttcgccacca
      781 ccggattcgg cgacgtcata gcgctcgatg ctgcgaccgg caaagagctg tggcggacca
      841 atgtcggcgt cccgatcttc aacgcgccga cggccaatgg cgggcgggtc ttcgtttctt
      901 cccaggacaa tcacttccac gccttcgccc aaaacaacgg ccgcgaattg tgggaccatc
      961 agggcatcac cgaagccgcc ggtattctgc agagcaccag cgccgccgtc gcgggcgaat
     1021 tcgtcatcgc cccctatacc tccggcgagc tctatgcgct gcgcgtgcag aacggacgcc
     1081 ccgcctggag cgacatgctc acgcgctccg gtaatgtgac cgcgctcacc gagctcgacg
     1141 acatcgccgg acggccggtc gtcgaccggg atttggtatt cgcgatcagc cattccggcg
     1201 tcatggcggc aattcagctc ggcaccggcg accggcaatg gacgcgcgac ctcgcctcca
     1261 tccagacacc ctgggtggcg ggggatttcg tctacgtcct gacgacgcaa gcgcagcttc
     1321 tctgtctcac gcgcaaagaa gggcgcgtga aatggcttca ccaattgccg cgctggaccg
     1381 accccaagga tacgagcagc gacccaatcg tgtggtcggg cccggtgctg gtgtcgaacc
     1441 gcttgatcat ggtctcgtcg gacggctacg ccgtctcggt gtcgccctac tcgggcgagc
     1501 tgctcggccg gatggaaata cccgccggcg cctacatcgc accggtcgtc gccgacgata
     1561 cgctctacat tttgaccaac gatgcggatc tcgtcgcgct gcggtgaaag gggcgagtag
     1621 cgaataggga gtagcgaata gaaagtttat attctttctt ttcttcgcta ttcgctattc
     1681 gctattcgcg ataatccatg cctctgacca tcgccatcgt cggccggccc aatgtcggca
     1741 aatccacgct cttcaacaag ctggcgggaa gaaaactcgc cctggtgcac gacaccccgg
     1801 gggtaacgcg cgaccggcgc gaagccgacg cgaggctggg cgcgctgtcg ctcaggctgt
     1861 tcgacacggc cggcatcgcg catggcgcga aggacagcct cgccgcgcgg atgacggccc
     1921 agaccatgac cgccatcgcc gacgccgatg tctgcctgtt catgatcgac ggacgcgaag
     1981 gcgtcaccgc cgccgacgag atcgtcgccg aggcggtgcg ccggtcgggc aagccggtcg
     2041 tgctcgccgt caacaaatgc gaagggcggc agacgcatct gggcgagggc gagggctggg
     2101 cgctggggtt cggcgagccg gtcgcgatct cggcggaaca tcgcttgggg ctggaagaac
     2161 tggtcgaggc gctggcgccg ttcgccaaag cggaagagcc ggtcgaagaa aacccggacg
     2221 gcgacaagcc gttgcgcctg gccatcatcg ggcggccgaa cgtgggcaaa tcgagcctgt
     2281 tcaactatct gctcggcagc gaacgggcgc tgacggggcc ggaagcgggc atcacccgcg
     2341 atgccatcgc cgcgcgctgg caggccgaag ggcgggagat tctgctgcac gacacggcgg
     2401 gactcaggaa aaccgcgtgc gccgccggcc acacgctgga ggaactgtcc gttgcctcga
     2461 cgctcgatgc cgtgcgcttc gccgattgcg tcatcgtggt gatcgaccag accgcgccgt
     2521 tcgaaaaaca ggatttgacc atcgcggaca tgatcgcgcg cgaaggccgc gcggcggtgt
     2581 tcgccgccaa caaatgggac ctggccgcca accgcgccgg cgccatcggc aggctgcgcg
     2641 aaaagctgga ccggctgttg ccgcagcttg ccggagcgcc gctgctcgcg gtttcggcgg
     2701 tgaccggcga agggatcgac cgcctgctgc cggcggtgct ggcggccgac cgcgcctgga
     2761 acacgcaagc ggctggcgcg cacggtgcgc tcggacaaat ttgcggtcac cagcgacacc
     2821 gctttcgccg aggtcatgct tgcctgcgcc gcgcccgcgc cggaccgcga agaaacctgg
     2881 atcaacgacg aaatcctgcg cctgtacaca gcgctgcacg ccagcggcca cgcccattcg
     2941 ctcgaatgcc ggcgcgacgg aaggctggtg ggcgggcttt acggcgtgcg gctgggcggc
     3001 gccttcttcg gcgagagcat gttctcgcgc cagaccgacg ccagcaaagt ggcgctggtc
     3061 catctggtgg cgcggctcaa gctcggcggc ttcaagctgc tcgacgccca gttcctgacc
     3121 gaacatctgg cgcgtttcgg cgcggtggaa attccgcgcg cggcgtacct tgccagactg
     3181 agcgaagcga tttccgccct ggcctattgg ccggcgtctt cgggtaattc gggaagggct
     3241 tcgttggcgc ccgcattggg cgacaccggc ttgaccgggg tcgaggccgt caccaacggc
     3301 gcctggtccg gcgcattggt cttgcagctg atcgcccaga cgtcatagag cggatgctgc
     3361 atcccgttca gcccggggct ggaagcatac atccagcccg agaagacgcg ccgctccggc
     3421 tggtccggac ggtgatcttc gatctgcaga aaggccgagg tctccggcgt ctcgctcgcc
     3481 ggggtcgaat agcaggtgcg cgccacgatc gtcagcgtcg cgtatttcac cggcacattg
     3541 agcggcgcca gcagcacgct cgcccgcccg gtgatcttgt cgagcgcgcg caattcgagg
     3601 accggacgcg cggcgctgcc cgtgcgcaat tcgggcgggg gcgtaagcat gatgttcatc
     3661 gacgcatccg gcggcgactg cgcctccgcc gccgcggcaa gcaccgccaa ggcggcgacg
     3721 gccgaacgca acatcatggc ctggtctctt cgctgttgcc gctgccgctc gccgcgccgc
     3781 cggcgaagcg cgacagcaga gccatcaaat cgacggaacc ttgcgtgttg cggatgcggc
     3841 cgtcggccgc cagcaccttg tcgctgccgc cgggcgcgat cgaaagatag gaactgccga
     3901 gcaggccgga cgacgtcacc atcaacgaag aatcgtcggg gatcttcacg tcgttgtgga
     3961 cgttcaaatg cacgatgacg agatagtcct tcggatcgag agtgagcgac gaaacctcgc
     4021 ccaccttgat gccggagagc ttcacgtcgg tgcccacggc gatgccgtca acccgcgcca
     4081 tcttggccgt cagttcatag cccttgacgg acgccgagcc cgtgctctga taggcgaaga
     4141 cgagaaagat aaccgccacc gccacgacgg cggcgccgat cagagtttca accaaatcgt
     4201 tgtgcatcgt tttctaaacc ggtttccagg gttcataatc tccggtcgcc ggcgcgcgca
     4261 cgccgccgcg cgccaagctt ccaggcggat aataggcgcc ttttgtgccg ctcatattgg
     4321 gcaaatgatc tttttcccag ggtttgatca gggcagggcc gccgggcggt tctttgaagg
     4381 tgtggtgcag ccagccgtgc cagtccgcag gcacgcgcga cgcctcaacc atgccgttat
     4441 acagcaccca gcgccgcttg ccgtccttgg tgcggtaata acggttgcca tacgcgtccg
     4501 tgccgacggg tacgcccttg agccaggtcg tcagcagcgt gccgtaagtg gcgccgcgcc
     4561 accaagcaaa gagcatccag aagaaacgca tgaccgcacc gatcagattc accggaatat
     4621 agaggcgcga cggcgtctta gccaagggag tggcggagac caacagccgc gcccctggat
     4681 tcggccaatc ctttgtaatc gctgcatatt gcggttgagg ttgttttcga gttttgcaag
     4741 taccccctcc ccatccccgc atctgccttc tccttcgagc ctccggaatc gtggggccgc
     4801 cctccatttc gtcaatattg aattcgaatc gtacattctt gctttaactt attgatttat
     4861 aaacacaaac acattctttc agagaaagtt ctttggcgca atttccattc cggcacggcc
     4921 ttctccagca gggcaattgc cttcgcaaat atcttccgcg gttttactcg ggcctcgccc
     4981 gcaagcgccg atgtaggaat gaaatccagc agttcaaggg ggtgtggcga aaaatattct
     5041 agagtcattt gggctggctt gaatcactct caccttcgac aaactaaggg tgaggggatt
     5101 ttgcgtccaa gtctcctaat ctcgacaata ttgtgaattc actatatagg tgaatctgga
     5161 tgacggagca actgccgttc agaccgctga tctgggtcgg atcgacaagg cggattttcc
     5221 gcgccatgcc ggccgaggtg aaatcccgta tgggttatgc gctctatgtg gcgcagcttg
     5281 gcggaaagca tcgggatgcc aggccgctga gaggatttgg cggcgccggg gtgttggaga
     5341 ttgtgaagga ccatcgcggc gacacgtttc gttcgatcta tacggtgcgc ctcgctggag
     5401 cggtttacgt gctgcatgtg tttcagaaga aatcgaagac cggtcgcgag acgcccaaaa
     5461 ccgacatgaa cctgatcgaa acaagactgc gcgaggcgga gcggatcgca aaggacgtgc
     5521 gaacatgaaa aagaaaaccg attatgagat gggaaccggc aatatcttcg ccgatgtcgg
     5581 tcttcccaat gccgaggagc atctggtcaa ggcccagctt gtctacagaa tcgacgggct
     5641 gatgaaggag cgcggcttga aacagatcga agcggcgaaa cttttcggcg tgaagcagcc
     5701 ggatgtttcc aaaatgctgc gcggcgattt ccggcaattc tccgtcgagc ggctcatgcg
     5761 ctttctcgta gccctcggcc agaatgtcga gatcgtggtg acgccgcatc gcggacgaaa
     5821 ggaaaccgcc caactgcgcg tggcgccggc cgttgcgtga aacgcggtcc cgagaaaccg
     5881 gggggggcag gtcagagaat taaatattgt gtccacaaaa cgctatccca gaatacttta
     5941 gcgatggcgc aagcatggca cggatgactg acccttcaga tgcgctgaac tcgttccaac
     6001 aagcacttcg tcacggtgag gtacggccgc agcgatgcga aactgattca aacttatttg
     6061 ttcatttgga ccatccgaac ggcgaagtcc ggatcacgta tgtaagaatt tatcacgaaa
     6121 cagtgacggc actggcgtcg ttcatcttgg gagcgccgat tgacgggctt ccttgtttca
     6181 gcatcgggta tgccgtcccg gagaaatttc gcaatcaggg tagagcaaaa gacattattg
     6241 aggcgggcat agctgaaatg aagtacggat tattctctcg cgccaacatt tcatcgttct
     6301 acgtcgaggc agtcattgga gtggacaata agccatctcg gcatgtcgct gcggcgacca
     6361 tctcattcaa cccacttcca gtgacggatc acgtttcggg gctgcctgcg ttccaatacg
     6421 tccgaaagat tgaaaacaat acggtt
//
LOCUS       DASWPU010000080         9892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_110730,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000080 DASWPU010000000
VERSION     DASWPU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110730"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..1111)
                     /locus_tag="VGC27_01775"
     CDS             complement(323..1111)
                     /locus_tag="VGC27_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0281332.1"
                     /translation="MISDLFRLDGCVAFVSGAAGHLGRSMTWALCEAGAHVIVNGRDD
                     ARLKDLEDELKRQGFSCERAAFDMGDIARVRAFFARSKRLDVLVNNAISMTPKSFAAL
                     EPDDFATTYASSVTAAFEAVRAALPALRKAVQAAGEARVVNIASMYASVAPDTRIYAK
                     PEQASPFHYGPAKAALLQLTRHLAAELGPEKIRVNALVPGPFPRPQVAVNDPAFAERL
                     AGRTMLGRLGETGEIAGPLLFLASKASSYVTGAALAVDGGWTQW"
     gene            complement(1108..2034)
                     /locus_tag="VGC27_01780"
     CDS             complement(1108..2034)
                     /locus_tag="VGC27_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0281333.1"
                     /translation="MGERSAELVLGSVQLGLAYGVANKTGKPSRRAALKLVRQAADAG
                     VTQFDTARAYGDSEERLGEALDGLAVRAITKLSPLADLPEGAGRDDVRRAVDASIAES
                     LAALRRKRIDCLLLHRAGHMTAFGGAVWERLLERLDDGSVRTLGVSVGSPPEALDALA
                     RREVRHIQMPFNLLDWRWREAGVIAALEKRPEVAVHARSVFLQGLLAANDPAIWPRIE
                     GVDPNAVLRLVAALAEEFGRESAADLCLAYARGQSFIDGVVIGLETMEQLDANLRLAV
                     KPPLAKEECALVEARMPRLPEILLNPALWPKS"
     gene            2320..3582
                     /locus_tag="VGC27_01785"
     CDS             2320..3582
                     /locus_tag="VGC27_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281334.1"
                     /translation="MKKNFVRAVVVASLSLFVSVATFTPTPAVAASADKDAKKPVVSR
                     AISKHVSEAQKAFEAKNWAAMLAKVKEAAAVPDLSDYDKYIVNYFMGLALYNSGDKTG
                     ATPYFVAAAESDAAPPEERDTALRIAIDLSNQAKDYAKVKSLGQLAVDAGALSGGVAA
                     IIAIAYYDSGDYPNAVAFAQKAIDIETAAGKLPDRSAYQVILMSQNRQKDIPGELKTL
                     EIMSTNYGNAEDWGHLIDISLGTLPKSGSRETAALYLYRLRLAVGAETSASDYQMAAD
                     LALALRYPGDAQKALQQGIDKGVLSQSAAAASLNRANAAARTDEPTLPAADAAAAKSP
                     SANGDVSVAGAYYGYGRYEDAIRVAQRAVTKSGTKAIEARLLLGVAQARQGDKAAAVQ
                     NLTQVRGDLALERAAQLWLAYANRKYTP"
     gene            3673..4227
                     /locus_tag="VGC27_01790"
     CDS             3673..4227
                     /locus_tag="VGC27_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281335.1"
                     /translation="MAQLIGFAPLVVFCVIARLSLSLALWLAFAMAFSLGIRSFLEAG
                     ILRLLDAAGMVLFGLLALYAGFIQRGIEISWVGMILETALLGLAVWSLTARRPFTAEY
                     AGERISPEQWDTPQFARAQYRLTGVWAVAFAAMAAADAQTLFLHAIAPNKTAAIGLAV
                     LAAALIYTWQSGIQIGRRFGKTPY"
     gene            4252..4893
                     /locus_tag="VGC27_01795"
     CDS             4252..4893
                     /locus_tag="VGC27_01795"
                     /inference="COORDINATES: protein motif:HMM:NF016189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HEY0281336.1"
                     /translation="MHFLKAVKPILRDLLSTILFVSLFWLTGNIFWATGFGIALGVAQ
                     TLWMRLSGRPVGFLQWLSLVLVTVFGTTTIVTHNPFYIMAKPTVVWLAVGAMMLRRDW
                     MAPYLPPIVTENLDDRLIVRAGYAWAAVMFVLAMLNLLVVVMTTQEFLSICATLAPDA
                     ALVIARTVTPKFWSVYALAGPAIAQLALITTQYFLFRKRVAGRIRARAATQAA"
     gene            5005..6210
                     /locus_tag="VGC27_01800"
     CDS             5005..6210
                     /locus_tag="VGC27_01800"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0281337.1"
                     /translation="MREAVIVSTARTGLAKSMRGGFNLTHGAVMGGHAIAHALAKAGV
                     DPAEVEDVAMGCALPEGATGMNIARLSAIRAGCPAATAGVTVNRFCSSGLQAIAMAAH
                     EIVSEGAPVAVGGGVESISLVQMGGMNTGNITEEWLMAHKPALWMSMIETAEIVAERY
                     AVSRERQDRYALESQRRTAAAQQAGRFDGEIVPLATRMKKFDKASGRESVVDVTVDRD
                     ECNRPDTTYEGLAALKPVLTAGMQVKEGKCITAGNASQFSDGAAACVLMEAAVAARKG
                     LTPLGIFHGFAVAGCEPDEMGIGPVFAVPRLLQRHGLGPGDIDLWELNEAFASQTLYC
                     MERIGIAHDKLNVNGGAISIGHPYGMSGARMTGHILLEGRRRKLRYGVITMCIGGGMG
                     AAGLVEFVH"
     gene            6212..6856
                     /locus_tag="VGC27_01805"
     CDS             6212..6856
                     /locus_tag="VGC27_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0281338.1"
                     /translation="MKTAHFLRVACCTVLLLFCAGCALFGPSKPDVPAQMPALESRIY
                     ALIVDQRRILDPKAQFLSLDPELVAIARKRSMDMARKNSFTADDLHASANMLMTDNAK
                     FQGLIGENVAAQHFTPGGTIDIEAFAGRFVEGWLASGPHKENLSFAEYDRTGVGAAAN
                     GDTIYVTQLFSTDLGLGAHGDSAPPSQVQPVASPQEGKDDQQKVPLRGDIAPGG"
     gene            6878..7246
                     /locus_tag="VGC27_01810"
     CDS             6878..7246
                     /locus_tag="VGC27_01810"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEY0281339.1"
                     /translation="MAEPSMDRRIETFDDFWPYYLRHHTKPQTRALHYLGTGLASLGL
                     VVFVVVGDPWGLLVVPVAGYGPAWIAHCFIEKNHPASISRPLWSLACDFRMAFSWLTG
                     NIAEDLERAGVEGTRDRPSR"
     gene            7385..9826
                     /locus_tag="VGC27_01815"
     CDS             7385..9826
                     /locus_tag="VGC27_01815"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281340.1"
                     /translation="MRPDLPWNVAGIPSEAREAARAAARREGLSIGEWLTRRILAGLS
                     GMSEPQDPPRDEWSSYRSEERGASRRDSEEMLDRVSRSETESAGVYRRIEEQLRGVGR
                     RLDAAERSQSESNRVMNKAAVEMNISAREQAQAFDQLGTHVVDISERLERVERNVGND
                     GLRDAVKALHQGLSRLADQITETANQSAAQISSLSGNLETLAGRLGQVRKETQASTQT
                     LESNIAALDERVRTVEKAAHANADALERALESLEARQPKDGSADAIARLEESIGRLEA
                     RVADPALDRRLSGIERALGDIVGRVDHDEEETRQSDVETDLKKLAQRLDAAEAAQRAA
                     LEEIRKGAAVKTEAKPPEPFPAFAEQPAPFQAMPAFDAPPFPEAAAPPPFADPGFVPP
                     PVPPPAYDTATGFTAEHPFTAGQGFAAEPPPPAVDSYLSAARRSARAASQAEAERATG
                     SFAGFGWNAAAKPAAPRSGRTLYLGIGVLALVAIAVIAGAVLNHRTAGEAPANSAIGA
                     LFEKKPPPPPIKAETSPAEDGNAAAPAAPATNQQIAPPLQKQEVKPAPVHMVPQNAAP
                     APQKTAPVPQTAAPAPQTAAPAPIAQKPPAKLPPQPAATAPLDRLTALANAGNAKAQL
                     VVALKYLDGDGVAANDTEAAKWLARAADAGEPVAQYRMGTLYERGRGVPADAAKAAHW
                     YQAAANQGNRKAMHNLAVAYAEGSGMKKDFTEASRWFSRAANLGLSDSQFNLAVLYER
                     GLGVPQSLLNAYRWYAIAGAQGDAESKARLQAISTQMSADDRTAAQRAADTFKPGPLD
                     ARANVAPTMEEALRR"
BASE COUNT         1755 a         3291 c         3166 g         1680 t
ORIGIN      
        1 accagttggc ttcggcgagc agccgcttca tcgcccccgc gccgacctcg gccagcagcg
       61 gaaaggcgct cagatgccgg tgcagggagt ccggataaac cgtctcctgc ccgctcagaa
      121 acgaggaaag aaaggccatg ataaccgccg ctctttctat ccttttagag cggatcagga
      181 acgaactgta tctctgattt caccatggcc gggcttgacc cggccatcca gcatagccgt
      241 tcactgcgtc cacgcttcgc ccggaccggg gggcggtcat gacgaagcgg ggcaagctca
      301 acgccgcacg gcgccgctca acctaccatt gggtccagcc gccatcgacc gcgagcgccg
      361 cgcctgtcac ataagaagag gctttgctcg ccaggaacag caaaggaccg gcgatttcgc
      421 ccgtctcgcc caggcgccca agcatagtgc gccccgccag ccgctcagcg aaggcgggat
      481 cgttcaccgc cacctgcgga cggggaaaag gccccggaac cagcgcgttg acgcggattt
      541 tctcgggccc cagctcggcg gcgagatggc gggtcagttg cagcagcgcc gccttggcgg
      601 ggccgtaatg gaacgggctg gcttgttcgg gcttggcgta gatgcgcgtg tcgggcgcca
      661 cgctggcgta catcgaagcg atattgacga cgcgcgcctc gcccgccgcc tgcaccgcct
      721 tgcgcagggc cggcagcgcc gcgcgcaccg cttcgaacgc agccgtcacg gaactggcat
      781 aggtcgttgc gaaatcgtcc ggctccagcg ccgcgaacga cttgggcgtc atcgagatcg
      841 cgttgttgac cagcacatcc agccgtttgc tgcgtgcgaa gaaagcgcgc acccgcgcga
      901 tgtcgcccat gtcgaaggcg gcgcgttcgc aagagaagcc ttggcgcttc aattcgtctt
      961 ccaaatcctt gagccgggca tcatcgcggc cgttgacgat cacgtgcgcg cccgcttcgc
     1021 acagcgccca cgtcatgctg cgtccgagat gaccggcggc accggagacg aacgccacgc
     1081 agccgtccag gcggaacaag tccgagatca tgattttggc catagcgcgg gattgagcag
     1141 aatttccggc aggcgcggca tccgcgcttc gaccagcgcg cattcttcct tggcgagcgg
     1201 aggtttcacc gccaggcgca gattggcgtc gagctgctcc atggtttcga ggccgatcac
     1261 gacgccgtcg atgaaacttt gtcctcgcgc ataggccagg cagaggtcgg cggcgctttc
     1321 gcggccgaat tcctccgcca gagccgcaac gagccgcagc acggcattgg gatcgacgcc
     1381 ctcgatgcgc ggccagatcg ccggatcgtt cgccgccagc aagccttgca gaaagacgct
     1441 gcgcgcatgc acggccacct ccgggcgctt ttccagcgcc gcgatcacac cggcttcgcg
     1501 ccagcgccaa tcgagcagat tgaacggcat ctggatgtgg cgcacctcgc ggcgggcgag
     1561 cgcgtccagc gcttccggag gcgaccccac cgagacgccc agcgtccgca cgctgccgtc
     1621 atcgagccgc tccagcagcc gttcccagac ggcgccgccg aaggcggtca tgtggccggc
     1681 gcgatgcagc aacaggcaat cgatgcgctt gcgccgcagc gcggcgaggg actccgcgat
     1741 gctggcgtcg acggcgcggc gcacatcgtc gcggccggca ccctcgggca gatcggcgag
     1801 cgggctgagt ttggtgatgg cgcgcaccgc gagtccgtcg agcgcttcgc ccagccgttc
     1861 ctcgctgtcg ccataggcgc gtgcggtgtc gaactgggtg acgccggcgt ccgccgcttg
     1921 gcgcaccagc ttcaaagcgg ctctgcggga gggtttgccg gtcttgttgg ccacgccgta
     1981 tgcaaggcca agttgtacgg aaccaagcac cagttcggcc gaacgttcgc ccatgaacga
     2041 ccccttatct gcctgtaaaa ggtctcagct tatggttaag gaagctctta cgaaccttac
     2101 ccaagcgcag tgaatcggcc ggagtgcgcg tcctcacgcc gctcaccgcg ttttcgtcac
     2161 aatctcctaa ggatggatgc cgccatattg caccgcagca gccatccgtc gcattggaaa
     2221 catcagacgg ccacaataat gttgtaaaag tctctcagat gcgcgaagcg gaagtgtggt
     2281 tgcgccctgt gtatcgcgcg gcagtcagaa gaggatccca tgaagaagaa tttcgtgcgc
     2341 gccgtcgtgg tcgcgagcct ttccctgttc gtttcagtcg ccaccttcac gccaacgccg
     2401 gccgttgccg ccagcgccga caaagacgcc aagaagccgg tcgtcagccg agccataagt
     2461 aaacatgtgt ccgaagcaca aaaggctttc gaggccaaga attgggcggc catgctcgcc
     2521 aaagtcaagg aggcggcggc cgttccggac cttagcgact atgacaagta catcgtcaac
     2581 tatttcatgg gcctggccct ctacaacagc ggcgacaaaa cgggggcgac gccctatttc
     2641 gtggccgccg cggaatcgga cgcggcgcct cccgaagagc gcgacacggc attgcgcatc
     2701 gccatcgacc tttcgaacca ggcgaaggat tacgccaagg tgaagtcgct tggtcaactg
     2761 gcggtcgacg ccggcgcgct gagcggcggc gtggccgcga tcattgcgat cgcctattac
     2821 gactccggcg attatccgaa cgccgtggcc ttcgcgcaga aggccatcga tatcgaaact
     2881 gccgccggca agcttcccga tcgcagcgcc tatcaggtta tactgatgtc gcagaaccgg
     2941 cagaaggaca ttcccggcga actcaagacg cttgagatca tgtccaccaa ttacggcaac
     3001 gccgaggatt ggggccacct catcgacatc tcgcttggca cgctgccgaa gtccggttct
     3061 cgcgaaaccg ctgcgcttta tctttatagg ctgcggctgg cagtaggcgc ggaaacttcc
     3121 gcctccgatt accaaatggc tgccgatctc gcgcttgcgc tgcgttatcc cggcgacgcg
     3181 cagaaggcgc tgcagcaagg catcgacaaa ggcgtcctga gtcaatccgc tgccgccgcc
     3241 agcctgaaca gggcgaatgc ggcggccaga accgacgaac cgacgcttcc ggccgccgat
     3301 gccgccgcgg ccaagagccc ctcggccaac ggggacgtct cggtggctgg ggcctattac
     3361 ggctacggcc gctacgaaga cgccatcagg gtcgcacagc gcgcggtgac caagtcgggt
     3421 accaaggcga tcgaagcgcg gttgctgctc ggcgtggcgc aggcgcggca aggcgacaaa
     3481 gcggccgccg tccagaacct gacccaggtg aggggcgacc ttgccctgga aagagcggcc
     3541 cagttgtggc tggcctacgc caaccgtaag tacaccccgt gagtccggcg aatcgggtgt
     3601 tcgcgctata gtgcgcgaac acccgggatt ccctggctcc atggttgtct tttcccgacc
     3661 cggaaggtgt tcgtggccca gttgatcggc ttcgcgccgc tcgtcgtctt ttgcgtcatt
     3721 gcacggctgt cgctgagcct ggcgctttgg cttgcgttcg cgatggcctt ttcgctcggc
     3781 atcaggtcgt ttctcgaagc cggcattctc aggctgctcg acgccgccgg catggtttta
     3841 ttcggtcttt tggcgctcta cgccggcttc attcagcgag gaatagaaat ttcctgggtc
     3901 ggcatgatcc tggaaacggc tttgctcggc ctcgccgtgt ggtcgctgac ggcgcgcagg
     3961 ccgttcacgg cggaatatgc cggggaacgg atttcgccgg agcaatggga cacgccgcag
     4021 ttcgcgcgcg cgcagtaccg cctgaccggc gtgtgggccg tggccttcgc ggccatggcg
     4081 gcggctgacg cccagaccct gttcttgcac gccatcgcgc cgaacaagac ggccgccata
     4141 gggcttgcag tgctggcggc ggcccttatt tatacgtggc aatccggtat tcagatcggc
     4201 aggcgcttcg gcaagacgcc ttattagaat tcacagaaca ggtaagcgcc aatgcatttt
     4261 ctgaaagccg tgaagccgat cctgcgcgac ctgctgtcga cgatcctgtt cgtctcgttg
     4321 ttttggctta ccggcaacat tttctgggcg accggcttcg gcatcgccct gggtgtcgcg
     4381 caaaccttat ggatgcggct gagcggccgc cccgtcgggt ttctgcagtg gttgagtctg
     4441 gtgctcgtga ccgtcttcgg caccacgacg atcgtcacgc acaacccgtt ctacatcatg
     4501 gcgaagccga cggtggtgtg gctcgcggtc ggcgcgatga tgctgcggcg cgactggatg
     4561 gcgccctatc tgccgcccat cgtgacggag aatctcgacg accggctgat cgtgcgcgcc
     4621 ggctacgcct gggctgcggt gatgttcgtc ctggcgatgc tgaacctgct ggttgtcgtc
     4681 atgacgacac aggagtttct gtcgatctgc gccacgctcg cgccggatgc cgcgcttgtt
     4741 atcgcccgca ccgtcacgcc gaaattctgg tcggtctacg cgctggcggg gcccgccatc
     4801 gcacagcttg cgctgatcac gacgcagtat ttcctgttcc gcaagcgcgt cgccggccgc
     4861 atccgcgccc gcgccgcaac ccaggccgcg tagccgccac atcctttggt gaggaaaagc
     4921 aaggcgctgc gtgccgggct ttcgtgtacc cggtcgaccc gctagggtcg ttcgcgagac
     4981 cttcgacaat tcgcggagaa cgccatgcga gaagccgtta tcgtttcgac ggcccgcacc
     5041 ggactggcaa agtccatgcg cggcggcttc aacctgactc acggcgcggt gatgggcggt
     5101 cacgccatcg cccatgccct cgccaaggcc ggcgtcgatc cggcggaggt ggaagacgtc
     5161 gcgatgggct gcgccctgcc cgaaggcgcc accggcatga atatcgcgcg gctgtcggcg
     5221 atccgcgccg gctgtccggc ggccaccgcg ggcgtgacgg tcaatcgctt ctgctcgtcc
     5281 gggctgcaag cgatcgcgat ggccgcccat gagatcgtca gcgaaggggc gccggtcgcc
     5341 gtcggcggcg gcgtcgaatc catctccctg gtgcagatgg gcgggatgaa taccggcaac
     5401 atcaccgaag aatggctgat ggcgcacaag ccggcgctgt ggatgtcgat gatcgagacc
     5461 gccgagatcg tcgccgagcg ctacgccgtc tcgcgcgagc gccaagaccg ttacgccctg
     5521 gaaagccagc gccgcacggc ggcggcgcag caggcgggcc gtttcgacgg cgaaatcgtc
     5581 cccctcgcca cccggatgaa gaagttcgac aaagccagcg gccgggaatc cgttgttgac
     5641 gtcacggtgg accgcgacga atgcaaccgc cccgatacga cctacgaggg cctggcggcg
     5701 ctcaaaccgg tgctgacggc cggcatgcag gtgaaagagg gtaaatgcat caccgccggc
     5761 aacgcctcgc aattctccga cggcgccgcc gcttgcgtcc tgatggaagc cgccgtcgcc
     5821 gcccgcaagg gtctgacgcc gctcggtatt ttccacggct ttgcggtcgc cggatgtgag
     5881 ccggacgaga tgggcatcgg tccggtcttc gccgtgccgc gcctgttgca gcgccacggc
     5941 ctggggcccg gcgacatcga tctgtgggaa ctgaacgagg ctttcgccag ccagacgctt
     6001 tactgcatgg aacggatcgg cattgcgcac gacaagctga acgtcaatgg cggcgccata
     6061 tctatcggcc atccttatgg tatgtcaggg gcgcgtatga ccggccatat tttgttggaa
     6121 ggaaggcggc ggaagttgag gtatggtgtc attaccatgt gcatcggcgg cggtatgggg
     6181 gctgcgggct tggtcgagtt cgtgcactga cgtgaaaacg gcacatttcc ttcgcgtagc
     6241 ctgttgtacg gtccttctgc tgttctgtgc cggctgcgcg ctgttcggac cgtccaagcc
     6301 ggatgtgccg gcccagatgc ccgcgctcga atcgcgcatc tacgcgctga tcgtggatca
     6361 gcgccgcatt ttggatccca aggcgcagtt cctttcgctc gaccccgaac tggtggcgat
     6421 cgcccgcaag cggagcatgg acatggcgcg caagaactcg ttcacggccg acgacctgca
     6481 cgcctccgcc aacatgctga tgacggataa cgccaagttc cagggcctga tcggcgagaa
     6541 tgtcgcagcc cagcatttca cacccggcgg aaccatcgac attgaagcct tcgccgggcg
     6601 ctttgtagag ggctggcttg ccagcggtcc gcacaaggaa aacctgagtt ttgcggagta
     6661 tgaccgcacc ggcgtcggcg cggcggcgaa cggcgacacc atctatgtga cccagctctt
     6721 ctccaccgat ctgggactcg gagcgcatgg cgacagcgcg cctccgtcgc aggtccaacc
     6781 ggttgcatcg cctcaagaag ggaaggacga tcagcagaag gtgccgctgc gcggcgacat
     6841 cgcgccgggc ggctgaaccg cataggatat cttgcgcatg gccgagccct ctatggaccg
     6901 tcggatcgag actttcgacg atttctggcc gtattatctg cgccaccaca ccaagcctca
     6961 gacccgtgcg ctgcactatc tgggaaccgg actggcctcg ctgggattgg tcgtcttcgt
     7021 ggtcgtcggg gacccatggg gccttctggt tgtgccggtg gccggttacg gaccggcatg
     7081 gatcgcccat tgcttcatcg aaaagaacca cccggcttcg atctcccgcc ccctatggtc
     7141 gctggcctgc gacttccgca tggccttcag ttggctgacc ggaaatatcg ccgaagactt
     7201 ggaacgcgcc ggggttgaag gcactcgaga ccggccaagc cgctaactac ctgttaattc
     7261 tgttagcaga tcgtcagggg atgacccttc ggaccccctt tgcgcttgat ttgtcgaact
     7321 tttctttaca ctacatcttg gcgccggtac tttctgtagg gggtactagg ccaggtgagt
     7381 cgaaatgcgg cctgatctac cttggaacgt ggcggggatt ccgtcggaag cacgcgaggc
     7441 ggcacgcgcg gccgcccggc gcgaaggctt atcgatcggc gaatggctca ccaggcgcat
     7501 cctggcgggg ctttccggca tgagcgagcc gcaagatccc cctcgcgacg aatggagctc
     7561 ctatcgttcc gaagagcgcg gcgcttcgcg gcgcgacagt gaagagatgc tcgaccgcgt
     7621 ttcgcgcagc gaaacggaat ccgccggcgt ctatcgccgc atcgaggaac agttgcgcgg
     7681 cgtgggccgc cgcctcgacg ccgccgagcg cagccagagc gagagcaacc gggtgatgaa
     7741 caaggccgcg gtggagatga acatctccgc gcgcgagcag gcgcaggctt tcgatcagct
     7801 cggcacgcat gtggtcgata tttccgagcg tctcgaacgg gtggagcgca acgtcggcaa
     7861 cgacggcttg cgcgatgcgg tgaaggcgct gcatcaaggc ctgtcgcggc ttgccgatca
     7921 gatcaccgag acggccaatc agtcggcggc gcagatttct tcgctctccg gcaatctcga
     7981 aacccttgcc ggccggcttg gtcaggtccg caaggagacc caggcctcga cccagaccct
     8041 ggaaagcaat atcgccgcgc tcgacgaacg cgtccgcacg gtggagaaag ccgcccacgc
     8101 caatgccgac gcgctcgaac gagcgctgga atcgcttgaa gcgcgccagc ccaaggacgg
     8161 ttcggccgac gccatcgccc gtctggaaga aagcatcggg cggctggagg cgcgcgtcgc
     8221 cgatcccgcg ctcgaccgcc gcctctccgg catcgaacgc gccctgggcg acatcgtcgg
     8281 gcgcgtcgat cacgacgaag aagaaaccag acagagcgac gtcgaaacag acctcaagaa
     8341 attggcgcag cgcctcgatg ccgccgaagc cgcgcagcgc gccgccttgg aggaaatccg
     8401 caagggagcc gccgtaaaaa ccgaagcgaa gccgccggag ccgttccccg ccttcgccga
     8461 gcagcccgcg ccgttccagg cgatgcccgc attcgacgcg ccgccgttcc ccgaagccgc
     8521 cgccccgccg cctttcgccg atcccggatt cgttccgccg ccggttccgc ctccggccta
     8581 tgacacggcc acgggcttca ccgccgagca tcctttcacc gccggccagg gtttcgccgc
     8641 agagcctccg ccgccggccg tggattccta tctttcggcc gcccgccgtt cggcgcgcgc
     8701 cgcctctcag gccgaggccg aacgcgccac ggggagcttt gccggcttcg gctggaacgc
     8761 tgcggccaag cccgccgcgc cgcgttccgg acgcacgctc tatctcggca tcggcgtgct
     8821 ggcgctggtg gcgattgccg tcatcgccgg cgcggtcctc aaccaccgca ccgccggcga
     8881 agcgcccgcg aactcggcca tcggcgctct gttcgaaaag aaaccgccgc cgccgccgat
     8941 caaggccgag acctcgcccg cggaagacgg caatgcggcg gcgcccgccg ctccggcaac
     9001 gaaccagcag atcgctccgc cgctgcaaaa gcaggaggtg aaaccggcgc cggtccacat
     9061 ggtcccgcaa aacgccgctc ccgcgccgca aaagaccgcc ccggtgccgc agacggccgc
     9121 tcccgcgccg caaacggccg cgccggcgcc tattgcccag aagcctcccg ccaagctgcc
     9181 gccgcagcct gccgcgacgg cgccgctgga tcgcctgacg gcgctcgcca atgcgggcaa
     9241 cgccaaggcc caactggtcg tggcgctcaa atatctggat ggcgacggcg ttgccgccaa
     9301 cgacacggaa gccgccaaat ggctggcgcg cgccgccgat gccggcgagc cggtggccca
     9361 ataccgcatg ggcacgctgt acgagcgcgg ccgcggtgtg ccggccgatg ccgccaaggc
     9421 agcgcactgg tatcaagccg ccgcgaatca aggcaaccgc aaggcgatgc acaatctggc
     9481 cgtggcctat gccgaaggct cgggcatgaa gaaggatttc accgaggcgt cgcgctggtt
     9541 ctcgcgggcg gcgaacctgg ggctgtccga ttcgcagttc aatctcgcgg tgctgtacga
     9601 gcgcgggctg ggcgtgccgc agagcctgct caacgcttac agatggtacg cgatcgcggg
     9661 cgcccagggc gacgccgaat ccaaggctcg tcttcaggcg atctcgacgc agatgagcgc
     9721 cgacgaccgc accgcggccc agcgtgccgc cgatacgttc aagcccggtc cgctcgacgc
     9781 gcgggccaac gtcgcgccga ccatggaaga agcgctgcgc aggtagccgc ttccgctgac
     9841 aagaaccgat cttcccttag cttgtcatgg cccgcgaaag cgggccatcc ag
//
LOCUS       DASWPU010000081         3602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_74035,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000081 DASWPU010000000
VERSION     DASWPU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74035"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..997)
                     /locus_tag="VGC27_01820"
     CDS             complement(71..997)
                     /locus_tag="VGC27_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0281341.1"
                     /translation="MNEPVLGGMAGTALDRAEPLFRKGNTNCGGCGMSSLMQMLRHAV
                     AERPVQFVIPACCAAVAGGTFPQATFGVPTLMTTFASAPAAASGVASVAELNGEDVRV
                     IVLAGDGGTFDIGFASLSAAAERGEDILYLCYDNEIYGNTGGQRSSATPAGAVTTNTP
                     EGKLEQKKDILAILAAHRIPYAASLSLAHADDTFRKLRYALDLKGFRFLHVLSPCPTG
                     WKSEPALGMELVRLAVRSGLFPVYEIFDGKRHVVNIEPDFSDEALDMYLSLQRRFQKS
                     GLSAADLRPAIDRYWQNLRALSGRIVPARRNV"
     gene            complement(997..2157)
                     /locus_tag="VGC27_01825"
     CDS             complement(997..2157)
                     /locus_tag="VGC27_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281342.1"
                     /translation="MTVQLLSANHGAALAATLAARANRRGRGFCSGVYPITPSTECME
                     YLCQQKIEKGRVVRVESEHSAMGVCIGAASAGARSFTATSSNGLAYMYENCIAAASLR
                     LPVVLAVANRTLGPPWNIWADHGDALTLRDHGLIQFYCADNQELFDTVLAAFRLAEDP
                     RVLMPALVNMEGFILSHTVAQTEIPGQEEVDRFLPPTDIPHRLGEVPHTLGQVEIPHQ
                     TEAHRWQHHSAMLEVPRIYADIQNEFEAVFGRRLADAAVAYRSEDADTLIVSMGTIGT
                     TAERVVDRLRAEGERVGSLRLRLFRPLPVEAIRAHYAGKRHIAVIDRNISLGLGGVLW
                     SETHCLADSGAVVQNYIAGLGGGDVRPEHIAAMLADLAPRKMAGPPSMMEAV"
     gene            complement(2154..>3602)
                     /locus_tag="VGC27_01830"
     CDS             complement(2154..>3602)
                     /locus_tag="VGC27_01830"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0281343.1"
                     /translation="EEAAAILFRTQPLPSVCGRVCPAPCMQACNREAFDGSVNIRSLE
                     RWIADHSTLQLVPQKAAQPLRVAVVGGGPAGLSAAYQLALRGHTVTIFEAGPALGGVL
                     RNGIPAFRLPEEMLARDVERILKLGIEARCHSRLGKDDMLRLKQDFDALIVCTGLGAS
                     FGLAAAGEKLDGVEQGLDFLDRVKRGPVSLHGNVVVIGGGNTAIDCARTALRCGAAQV
                     KIVYRRGREDMPAIAEEINDAKLEGVQLLTHRQPVAFSGGSAVSAVAIAEVEAGPPDA
                     SGRRRPLVTERISSLPCDKVLLALGQKSELDLLPESWTVREGRAWEDGQLLPVWFAGD
                     CASGEGTVTHAIGNGRRAALAVLMNPAAVPGETREDAAKPVAPGQIRFSHFEVAPPHS
                     DRQLAASARRDNFAEVNLGLTGPEEAERCFSCGHCTFCDTCLIYCPDGVIMRSENGYR
                     IDGEYCKGCGMCVAECPRSAIQMQEKNHEVSA"
BASE COUNT          640 a         1222 c         1153 g          587 t
ORIGIN      
        1 gggggcggct aatccctcat gctgagctgt cgaagcatga agggaacaag agccacacgt
       61 ctactccccc tcatacgttg cgccgggccg gaacgatcct gcccgagagc gcgcgcagat
      121 tctgccaata gcggtctatc gccgggcgca ggtcagcggc cgaaagcccc gatttctgga
      181 agcggcgctg caaggacaaa tacatatcca gtgcctcatc ggagaaatcc ggctcgatgt
      241 tcaccacatg gcgcttgccg tcgaagattt cgtacaccgg gaacaggccg gagcgcaccg
      301 ccagccgcac cagctccatg cccagcgccg gctccgactt ccacccggtg gggcaaggcg
      361 agagcacatg cagaaagcgg aagcccttga gatcgagcgc ataacgcagc ttgcgaaaag
      421 tgtcgtcggc atgggcgagc gacagcgagg cagcataggg gatgcggtgc gccgccagga
      481 tggcgaggat gtcctttttc tgttccagct tgccttcggg cgtgttggtg gtgacggcgc
      541 cggcgggcgt ggccgaagaa cgctggccgc cggtgttgcc gtaaatctcg ttgtcgtagc
      601 agaggtagag aatgtcctcg ccccgctccg ccgccgccga caggctggca aagccgatat
      661 cgaaggtgcc gccgtctccg gccagaacga tcacgcgcac atcttcgcca ttgagctcgg
      721 ccacgctggc caccccgctc gccgccgcgg gcgcggaggc aaacgtcgtc atcagcgtgg
      781 gcacgccgaa ggtcgcctgc ggaaaggttc cccccgctac cgcggcgcaa caggccggaa
      841 tgacgaactg caccggccgc tccgccacgg cgtgccgcag catctgcatc aggctggaca
      901 tgccgcagcc gccgcaattg gtgttgccct tgcgaaacag aggttcggcg cgatcgagag
      961 ccgttccggc catgccgccc agcaccggtt cgttcatcac acggcctcca tcatggacgg
     1021 cgggccggcc atcttgcgcg gcgcaagatc ggccagcatg gcggcgatgt gctcgggacg
     1081 cacgtctcct ccgcccaacc ccgcgatata attctgcacc accgcgccgg agtccgcgag
     1141 gcaatgcgtt tccgaccaca gcacgccgcc gagaccgagg ctgatgttgc ggtcgatcac
     1201 cgcgatgtgc cgcttgccgg cgtagtgcgc ccggatcgct tccacaggca gcggacggaa
     1261 caagcgcagc cgcagagaac cgacccgctc tccttcggcg cgcagcctgt ccaccacgcg
     1321 ttcggcggtg gtgccgatgg tgcccatcga gacgatcagg gtatccgcat cctcgctgcg
     1381 ataggcgacc gcggcatcgg ccaggcggcg gccaaacacg gcttcgaact cgttctggat
     1441 atcggcgtaa atccgcggca cctccagcat ggccgagtgg tgctgccagc gatgcgcttc
     1501 ggtctggtgc gggatttcaa cctgccccag cgtgtggggc acctcgccga ggcgatgggg
     1561 gatgtcggtc ggcggtagaa agcggtccac ctcttcctgc ccgggaatct cggtctgggc
     1621 tacggtgtgc gagaggatga aaccctccat gttgaccagc gccggcatca gcacgcgcgg
     1681 gtcttccgcc agccggaaag ccgcgagcac ggtatcgaaa agttcctgat tgtcggcgca
     1741 gtagaactgg atcaggccgt ggtcgcgcaa ggtcagggca tcgccatgat cggcccagat
     1801 attccagggc ggaccgaggg tgcggttggc caccgccaat accaccggca ggcgcaagct
     1861 cgctgcggcg atgcagtttt catacatgta agccagcccg tttgaagagg tcgcggtgaa
     1921 gctgcgcgcc ccggcggaag cggccccgat gcacacgccc atcgcgctgt gttcgctctc
     1981 cacccggacg acccgtccct tctcgatctt ctgctggcag agatactcca tgcattccgt
     2041 ggaaggcgtg atcggataaa cgccgctgca aaaaccgcgc cccctgcggt tggcgcgcgc
     2101 cgccagcgtc gccgccagag cggcgccgtg attggcgctg agcagttgga cggtcatgcg
     2161 ctcacctcat ggttcttctc ctgcatctgg atcgcgctgc gcgggcattc ggccacgcac
     2221 atgccgcagc ctttgcagta ttcgccgtcg atgcgatagc cgttctcgct gcgcatgatc
     2281 acgccgtccg ggcaatagat caggcacgta tcgcaaaacg tacaatggcc gcaggaaaag
     2341 caacgctccg cttcttccgg gcccgtcaga ccgagattga cttcggcgaa attgtcccgg
     2401 cgggcggatg ccgccaattg cctgtcgctg tgcggcggcg ctacctcgaa atgggaaaag
     2461 cggatttgcc ccggcgccac cggtttggcc gcgtcttctc gcgtttcacc cggcacagcg
     2521 gcgggattca tgaggacggc cagcgcggcc cgccgcccat tgccgatggc atgggttacc
     2581 gtaccttcgc cgctggcgca gtccccggcg aaccacaccg gaagcaactg gccgtcttcc
     2641 caggcgcggc cctcgcgcac agtccagctt tcgggcagca gatccagttc gctcttctgg
     2701 ccgagcgcca ggagaacctt gtcgcaggga aggctcgaga tgcgctcggt gaccagcgga
     2761 cggcggcggc cgctggcatc gggcggcccg gcttccactt ccgcaatcgc cacggcggac
     2821 accgcgctgc cgccagagaa cgcgaccggc tggcggtgcg tcagcaactg cacgccttcg
     2881 agcttggcgt cgttgatctc ttcggcgatc gccggcatgt cctcgcggcc gcggcgatag
     2941 acgatcttca cttgcgcggc gccgcagcgc agcgccgtgc gggcgcaatc gatcgccgtg
     3001 ttgccgccgc cgatcaccac gacatttccg tgcagcgaga cgggaccgcg cttgacccga
     3061 tcgaggaaat cgagaccttg ctccacgccg tccagcttct cgcctgccgc cgccaggccg
     3121 aaggacgctc ccaatcccgt gcagacaatc agcgcatcga aatcctgctt gagacgcagc
     3181 atatcgtcct tgcccaaacg ggaatggcag cgcgcttcga tgcccagctt caggatgcgt
     3241 tcgacatcgc gcgccagcat ttcttccggc aaacgaaacg ccggaatgcc gttacgcagc
     3301 acgccgccca gcgccgggcc ggcctcgaaa atcgtaaccg tgtgcccgcg cagagcaagc
     3361 tgataggcgg cgctcaatcc ggcaggtccg ccgccgacca cggcgacgcg caaaggctgc
     3421 gccgctttct gcggcacgag ttgcagagta gaatggtcgg caatccagcg ctccaggctg
     3481 cggatgttca cggacccgtc gaaggcctcg cggttgcagg cctgcatgca cggcgccggg
     3541 caaacgcggc cgcacaccga gggcagcggc tgggtgcgga acagaatcgc cgccgcttcc
     3601 tc
//
LOCUS       DASWPU010000082         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_104627,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000082 DASWPU010000000
VERSION     DASWPU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104627"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VGC27_01835"
     CDS             <1..349
                     /locus_tag="VGC27_01835"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281344.1"
                     /translation="HRLSRADMDKIVDIQIGHLQKLLADRKIEIALDAKAREWLAGAG
                     YDPVYGARPLKRVIQRRLQDPLAQLLLEGKIADGAKVKVVAGKGGLVINGQEFAASSD
                     EVEFENAPSRALN"
     gene            complement(476..>1797)
                     /locus_tag="VGC27_01840"
     CDS             complement(476..>1797)
                     /locus_tag="VGC27_01840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0281345.1"
                     /translation="AQSRETAPSQLDRALVAEQADNDADPGVETHTMTLDPGDTLAGA
                     LGEAGVSIADANAAIAALTKVYNPRSLRAGQSFDLSFATARGADRSASQAGLVDASSQ
                     GDSANFADAPSAAEPTASLLSISFSPSIEHDITVTRAADGSYLANDAVKQLVSHVHRA
                     GATIDSSLYLSAMQAGIPADVVVEMIHMFSYKVDFQRDIRPGDSYEVFYDYYYTPQGQ
                     PAKAGNISYAMMKLGGREIALYRYQPDPKQPADYFDSHGQSAKGMLMKTPVDGARISS
                     GFGMRFHPVLGYTRMHKGIDFAVPIGTPVMASGSGVIVVRRWENGYGNFVRINHGNGY
                     STGYGHLSRFVAGLHTGSRVRQGQIIAYSGMTGIATGPHLHYETSVNNSQVNPLKLKI
                     AQGRKLAGHELRDFLAMRLHIDAVLASTKLETKVADNSADLRQAKAK"
BASE COUNT          341 a          539 c          580 g          337 t
ORIGIN      
        1 ccaccggctc tctcgcgccg acatggacaa gatcgtcgat atccagatcg ggcatctgca
       61 aaagctgctc gccgaccgca agatcgagat tgcgctcgac gccaaggcgc gcgaatggct
      121 ggcgggcgcc ggctacgatc cggtgtacgg cgcgcggccg ctcaagcgcg tcatccagcg
      181 ccggctgcag gatccgctgg cgcagttgct gcttgaagga aagatcgccg acggcgccaa
      241 ggtgaaggtc gtcgccggaa aaggcggact cgtcatcaac ggtcaagaat tcgccgcgtc
      301 atccgacgag gtcgagttcg aaaacgcacc cagccgggcg ctgaactgac ggccggccgg
      361 cgataatttg aatcattccg ccgaatgcga cggcaagaag gatcacgacg ttttcatcgc
      421 ccgccggcaa caatcctgta ttgatccgtg gtcagactga agccggtact gtgcgctatt
      481 tcgccttcgc ctgcctaagg tcggcggaat tatccgcgac cttggtttcg agcttggtgg
      541 aagccagcac ggcgtcgatg tgaagccgca tggcaaggaa atcccgcagc tcgtggcctg
      601 ccagtttgcg gccctgggcg atcttcagct tcagcgggtt gacctgactg ttattgacag
      661 aggtttcata gtgcaggtga ggcccggtgg cgatgccggt catgccgcta taggcgatga
      721 tctggccttg gcgcacccgt gaaccggtat gcagccccgc cacgaaacgc gacaaatgtc
      781 cgtatccggt cgagtagccg ttgccgtgat tgatccgcac gaagttgccg tagccgtttt
      841 cccaacggcg cacgacgata acgcccgaac ccgaagccat cacaggcgtg ccgatgggaa
      901 cggcgaagtc gatccccttg tgcattctcg tatagccaag gaccggatgg aacctcatgc
      961 cgaacccgga agagatgcgc gcgccatcga cgggcgtctt catcaacatg cccttggcgc
     1021 tctgaccgtg ggagtcgaaa tagtccgctg gctgcttggg atccggctga tagcgataga
     1081 gcgcgatctc gcggccgccc agcttcatca tcgcatagga gatgttgcct gccttcgcgg
     1141 gctggccttg cggcgtgtag taataatcgt agaaaacctc gtaggaatcg ccggggcgga
     1201 tgtcgcgctg gaagtccacc ttgtacgaga acatgtgaat catctcgacc acgacatcgg
     1261 ccgggatgcc cgcctgcatc gcggagagat aaaggctgga gtcgatggtc gcgccggcgc
     1321 gatggacatg cgaaacaagc tgcttcaccg cgtcgttggc gaggtagctg ccgtcggcgg
     1381 cgcgcgtgac ggtgatgtcg tgttcgatcg acggagaaaa ggaaatggac aacagcgagg
     1441 cggtcggctc cgccgccgac ggcgcgtccg caaaattggc gctgtcgccc tgggaagacg
     1501 cgtccacgag accggcctgg ctggcggatc ggtccgcgcc ccttgccgtc gcgaagctta
     1561 ggtcgaaaga ttgcccggct cgcaggctgc gcggattgta gactttcgtc aacgccgcga
     1621 tcgcggcatt ggcgtcggcg atggaaacgc cggcctctcc caatgccccc gcaagcgtgt
     1681 cgccgggatc gagcgtcatc gtatgcgttt caacgccggg atcggcgtcg ttgtccgcct
     1741 gctcggcgac caatgctctg tcgagctggc ttggtgcggt ctctctgctt tgggcag
//
LOCUS       DASWPU010000083         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_25046,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000083 DASWPU010000000
VERSION     DASWPU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25046"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VGC27_01845"
     CDS             <1..256
                     /locus_tag="VGC27_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281346.1"
                     /translation="LDTDTNNISVPDYFRADFDVSYDLDKLGMEGSSIQLNIDNLFDK
                     HYYGNIGTQSCYTGTTGCTSSPYVSPAALRTISVTLTARY"
     gene            388..1383
                     /locus_tag="VGC27_01850"
     CDS             388..1383
                     /locus_tag="VGC27_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281347.1"
                     /translation="MRLLFSILLAAALASSSALAWGSDGHRIIGELAVKSLPADLPAF
                     LRTSAAAKEAGYLAPEADRRRGAGEAFDAEHSPAHFVDVSDDLTVLGGPKLDALPPTR
                     EQYDTALRAAGSNQYKAGYLPYSMIDGFQLLAKDFAYWRVDVAGAKLGKSAEARAWYA
                     KDRVEREKIILHDLGIWAHFVGDASMPLHASVHYNGWGDFPNPEGFTTERVHVPWESR
                     FVHEQISDKDVAAAIPPARDCACTIAVRVAEFLAGTQAEVVPFYRLEKAGAFKTATPD
                     GKAFTVRQVARGAGELRDLIVAAWNESEKQSVGYPPVKVSDVEAGKADPYAALSY"
     gene            1422..>1618
                     /locus_tag="VGC27_01855"
     CDS             1422..>1618
                     /locus_tag="VGC27_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281348.1"
                     /translation="MFRLRPIRKQAKAPRILRGVEAAFWFVLALAVGVGGPTALSGLP
                     RLGALLVPGGMFAAEARGEPKT"
BASE COUNT          283 a          510 c          532 g          293 t
ORIGIN      
        1 tctcgacacg gatacgaaca acatcagcgt tcccgactac ttcagggccg atttcgacgt
       61 gtcctacgac ctcgacaagc tgggcatgga aggctcgagc atccagctca acatcgacaa
      121 cctcttcgac aaacactatt acggcaacat cggtacgcag agctgctaca ccggcacgac
      181 gggctgtacg tcgtccccgt acgtgtctcc ggcggcgctt cgcaccatct ccgtcactct
      241 gacggcgaga tactgatccg ttacggttct tccgtttcac cggccgggag caatcccggc
      301 cggtcttttt tatttgtctt tgcgcggctg gccgcttgcg gcaagatcgg cttaagcgga
      361 aaaaggagtc gggtgatggg tgctcggatg cggttgctgt tttcgatttt gctggcggcg
      421 gcgctggcgt cgtcttcggc tttggcctgg ggctcggacg gccaccgcat catcggcgag
      481 ctcgccgtga agtctctgcc cgccgatctg cccgccttcc tgcgcacgtc ggcggcggcc
      541 aaggaagccg gctatctggc gcccgaagcg gatcgccggc gcggggcggg cgaagcgttc
      601 gacgccgagc atagcccggc gcatttcgtc gatgtctcgg acgatctcac cgtcctcggc
      661 gggccaaaac tcgacgcgct gccgccgacg cgcgagcagt acgacaccgc gctgcgcgcc
      721 gcaggatcca atcagtacaa ggcggggtac cttccttatt cgatgataga cgggttccag
      781 ttgctggcga aggatttcgc ctattggcgc gtcgatgtcg cgggcgcgaa actcggcaag
      841 agcgccgagg cgcgcgcctg gtacgcaaag gacagggtgg agcgcgagaa gatcattctc
      901 cacgatcttg gaatctgggc gcatttcgtc ggcgacgcca gcatgccgct gcacgccagc
      961 gtgcattaca acggctgggg cgattttccc aaccccgaag gcttcaccac ggagcgcgtc
     1021 catgtgccgt gggaaagccg gttcgtccac gaacagattt cggacaagga cgtggcggcg
     1081 gccatacccc ccgcccgcga ctgcgcctgc acgatcgcgg tgcgggtcgc ggagtttctg
     1141 gccggcacgc aggccgaagt ggtgccgttc taccggctcg aaaaagccgg ggccttcaag
     1201 acggcgacgc cggacggcaa agccttcacg gtacggcagg tcgcgcgcgg cgcgggcgag
     1261 ctgcgcgatc tgatcgtcgc cgcctggaat gaaagcgaaa agcagtcggt cggctatccg
     1321 cccgtcaaag tatccgacgt cgaagccggc aaggcggacc cgtatgccgc actgtcttat
     1381 tgagacattg acagaaacgg ccggtcaagc aaggcatggt catgttccgc ttgaggccga
     1441 ttcgtaagca agcaaaggcc ccccgaattc tgcgcggcgt cgaggcggcg ttctggttcg
     1501 tgctggcgct ggccgtaggc gtaggcggac cgaccgcgct gtccggcctg ccgcggcttg
     1561 gcgccttgct ggtgcccggc ggcatgttcg cggcggaagc gcgcggcgag ccgaagac
//
LOCUS       DASWPU010000084         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_6786, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000084 DASWPU010000000
VERSION     DASWPU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6786"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="VGC27_01860"
     CDS             <1..1614
                     /locus_tag="VGC27_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281349.1"
                     /translation="FVGVADKGAAGLYRSDDAGMSWKKVPGGPGPELLPGQGQFDAKT
                     GKLYITYADSMGPYGASKGAVFQYDVKTGAWTDITPPKKAAYMGLALDARKPGTLVVA
                     TLDLDDTSGDSIYRSTDGGAHWVDLRPLSKRDVSSVPWLLWGKEEAKFGWWITGIAID
                     PFDSERAAYTTGATIYATDHLSRSEKGEELLWKPWVEGVEQTAVLTLAGPKEGAHLIS
                     GFGDIGGFTHSDLAAATMMHTNPIFVNTNTIDIAGQNPAVIVRSGSHEAHARVKTATL
                     GYSTDYGKTWQPLFAPPPAGYTVRAPEDIPYNFSDPYIDAAIITAADGKTFMVMIPDG
                     PVITTDLGQSWIKVKGLPPRARPVADKVDPKRFYALDFATGRVFLSVDGGASFQPQKT
                     KGLSVTAADDPFAGSGWPPVRERPWPLISADEKAGELWLIHQGRLYRSTDGGAHFAKV
                     KSDLDVRIIDFGKAAPGAQELAMYALGSKGGVFAIWQSLDRGAHWTRINDADSEYCRM
                     FRAIAADKKVFGRVYVGIDGRGIVYGEPK"
     gene            1914..2603
                     /locus_tag="VGC27_01865"
     CDS             1914..2603
                     /locus_tag="VGC27_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY0281350.1"
                     /translation="MFLGKQTLINLAFLMVGIGLGIGGLKIKQATQSQLPPTPIVGPG
                     AVQYDIEVDRQVARSQLTDGAALFFGDSHTVALAISTVTARGENFGISSDTLDGLSTR
                     LPRYKIDKACAVILEIGTNNIQKEADNGFARKYGRLFDHIPTTTPIIAVAIFPFLDPQ
                     YSAAVHTANLSIASACRSRPNCRVLDISDRLKADGQLDDRNYDSDRIHLSSRGTALWV
                     EALKASIPNCR"
     gene            complement(2747..>3491)
                     /locus_tag="VGC27_01870"
     CDS             complement(2747..>3491)
                     /locus_tag="VGC27_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281351.1"
                     /translation="APLVLLTESASFDPDIVAKIDAQLKRGGNVVITSGFLKAMQEKY
                     PAGGFRHIAEWTDTGRKALIGDFMQGYGAGNGQNLNDAKPAPILFPEIRFWTNDSWGI
                     IRGLAGAKGYPILLMNRYAKGIVYLWTMPENPADLYRLPQGVVTQVKAFLMPQTALRL
                     ESPAHVALFAYDNHAYVVESYRDTPVTVTLSLAEPGLVDAESGKTLAAETAPAVVPDP
                     SRHGFQPPKRTLYRVTVPPHSWRVFKRAP"
BASE COUNT          691 a         1110 c         1067 g          623 t
ORIGIN      
        1 ttcgtcggcg tagccgacaa gggcgcggcc ggcctctatc gcagcgacga tgcggggatg
       61 agctggaaga aggtgccggg cggccccggc cccgaactgc tccccggcca gggccagttc
      121 gatgccaaaa ccggcaagct ctatatcacc tatgccgaca gcatggggcc ttacggcgcc
      181 agcaagggcg ccgtcttcca atacgacgtg aagaccggtg cctggaccga catcaccccg
      241 ccgaaaaagg cggcctatat gggcctcgcc ctcgatgcgc ggaagccggg cacgctggtg
      301 gtggcgacct tggacctcga cgacaccagc ggcgattcca tctatcgctc gaccgatggc
      361 ggcgcgcatt gggtcgattt gcgcccgctc tccaagcgcg acgtatcctc ggtgccatgg
      421 cttctctggg gcaaggagga ggcaaagttc ggctggtgga tcaccggcat cgccatcgat
      481 cccttcgaca gcgagcgcgc cgcctacacc accggcgcca ccatctatgc caccgatcat
      541 ctttccaggt ccgagaaagg cgaagagctt ctctggaagc cctgggtcga aggggtggag
      601 cagacggcgg tcttgacgct ggccggcccc aaagaaggcg cccatctcat cagcggcttc
      661 ggcgatatcg gcgggttcac ccattccgat cttgccgccg ccacaatgat gcacaccaac
      721 ccgatcttcg tgaacaccaa caccatcgac atcgcggggc aaaacccggc ggtgatcgtg
      781 cgttccggtt cgcacgaggc ccatgccagg gtgaagacgg cgacgctcgg ctactccacc
      841 gattacggca aaacctggca gccgctcttt gcgccgccgc cggcggggta taccgtgcgc
      901 gcgccggaag acattcccta taacttctcc gatccctata tcgacgccgc gatcatcact
      961 gctgccgacg gcaagacctt catggtgatg atcccggacg gaccggtgat caccaccgat
     1021 ctcggccaga gctggatcaa agtgaagggc ctgccgccgc gcgcgcgccc ggtcgccgac
     1081 aaggtggatc cgaagcgctt ctatgccctc gacttcgcta ccggccgcgt ctttctcagc
     1141 gtcgatggcg gggcaagctt ccagccgcag aagacgaagg gtttgtccgt caccgccgcc
     1201 gacgatccct tcgccggctc gggctggccg ccggtgcgcg agcggccctg gccgttgatc
     1261 tccgccgacg agaaggcagg cgagttgtgg ctgatccatc agggcaggct ctaccgctcg
     1321 accgatggcg gcgcgcattt cgccaaggtg aaaagcgacc tcgacgttcg gatcatcgac
     1381 ttcggcaaag cggcaccggg agcgcaggaa ctggcgatgt acgcgctcgg ctccaagggc
     1441 ggcgtcttcg ccatctggca atcgctcgac cgcggcgcgc actggacgcg gatcaacgat
     1501 gcggacagcg aatattgccg catgttccgc gccatcgccg ccgacaagaa ggtattcggc
     1561 cgggtctatg tcggcatcga cgggcgcggc atcgtatacg gcgaaccgaa atagcgccgc
     1621 tcatagcagg cgggccgctc gaacatctgc ccggcggtgc cccggtcggc gccgcgggca
     1681 gacactcggc atgggccgga ttgcggccga gcggcgcctc gaacaacgcc gtattgccgg
     1741 ccgagccatc cgctccggta tcgcggaagg cggcggtcgc cacgccattc cccttgcagg
     1801 acatcgagcg caggcgcccc tgttcgccca tggctggcgg ccgactttca tagacaaaga
     1861 gggtgatata ggctgcgaca gacgaagacg cagattaacg catgaggccc gagatgtttt
     1921 tggggaaaca aaccctgatc aacctcgctt tcctcatggt tggaatcggg ctcggaattg
     1981 gcgggctcaa gatcaaacaa gcaacccaat cccaactacc gccaacaccc atagttggcc
     2041 ctggtgccgt ccaatacgac atagaggttg atcggcaagt ggcgcggtcc cagttgaccg
     2101 atggcgctgc actctttttt ggtgacagcc ataccgttgc cctcgccatt tcgacagtga
     2161 cggcacgagg cgaaaatttc ggcattagca gcgacacgct ggacggactg tcaacgagat
     2221 tgccgcgcta caagatcgat aaggcatgcg ccgtcatcct ggaaatcgga acgaacaaca
     2281 tccaaaaaga ggcggacaac ggcttcgcta gaaagtatgg ccgcctcttc gatcatattc
     2341 ccacgacgac gcccattatc gccgtcgcga tatttccctt cctagaccct caatattcgg
     2401 cggcagtcca taccgccaac ctatcgatag cgtcggcatg tcgaagccgc ccaaactgcc
     2461 gtgtgctcga tatttctgat cgcctgaagg cagacgggca attggacgac agaaattacg
     2521 acagtgatcg cattcatctt tcatcacgcg gaacagcact gtgggttgaa gccctgaagg
     2581 cgtcgattcc aaattgcaga tagcgactct ccgcaaatgc aagaggctcg gcagggagac
     2641 ctggacgctg tcctaataag cccttgagtg ttctaatttt ccttatcaag tgtccgaatt
     2701 aggacaacac ccgccgctgc gtcgatcctg cgatcgcggc gagtgcttac ggcgcccgtt
     2761 tgaacacacg ccaggaatgg ggcggcaccg tcacgcggta gagcgtccgc ttgggtggtt
     2821 gaaacccgtg ccggctcgga tcgggtacga ctgccggcgc cgtctcggcc gcgagcgtct
     2881 tgccgctttc ggcatcgacc agccccggct ccgccagcga tagcgttacc gtgacgggcg
     2941 tatcgcgata ggattccacg acataggcat ggttgtcgta cgcgaacagc gcgacatgcg
     3001 ccggggattc caggcgcagc gccgtctgcg gcatcagaaa ggctttcacc tgggtgacga
     3061 cgccttgcgg cagccggtaa agatcggccg ggttctccgg catggtccag agatagacga
     3121 tacccttggc gtagcgattc atcagcagga tgggatagcc cttggcgccc gccagtccgc
     3181 ggatgatgcc ccaggaatcg ttggtccaga aacggatctc cgggaacaga atgggcgcgg
     3241 gcttggcatc gttgaggttc tgcccgttcc ccgcgccgta gccttgcatg aaatcgccga
     3301 tcagcgcctt gcggccggta tcggtccatt cggcgatgtg gcggaagccg cctgcgggat
     3361 acttctcctg catcgccttg aggaagcccg aggtgatgac gacattgccg ccgcgcttca
     3421 gttgcgcatc gatcttggca acgatgtcgg gatcgaagct ggcgctttcg gtcagcagca
     3481 ccagcggcgc a
//
LOCUS       DASWPU010000085         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_37487,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000085 DASWPU010000000
VERSION     DASWPU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37487"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VGC27_01875"
     CDS             <1..853
                     /locus_tag="VGC27_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEY0281352.1"
                     /translation="NATAGLGYNLSARYIQPMFLEINQTLEFDLSGIKQQLDAYDQRA
                     ETIGVFVRRKFSPLWSAGAGLTLTHDDITQEGTKRLYQLLALPLTGSYDSTGVIDGLR
                     DPTTGLRASLALTPTHAFGAANLNFLPVQISASSYFDIGGDGRSVIALRALAASILGG
                     SHLELPPDQRLYVGGSATVRGFAYQSIGPQFADGKPIGAKSVDAGTIEFRQRIGEDWG
                     AVAFFDAGQAGKGGTPFSGTPRVGAGVGARYYTPIGAVRLDVAVPLTRMPRGDAFELY
                     IGLGQAF"
     gene            853..>2761
                     /locus_tag="VGC27_01880"
     CDS             853..>2761
                     /locus_tag="VGC27_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281353.1"
                     /translation="MREGRNIVRRAGLWSLAIAACVAVLLGALLYTPAGLAWSGRLVR
                     PLSDGTVTVIGLGGVFPNALHADAVEIADTEGVWLRLEQVTLHWSALPALNNHIVVQS
                     VIAANIRVLRRPLPSETSQGKTPRIDIEHFSAPHIEIAAPLLGRSAVLAAEGTLHYQS
                     RHQLAADIAVTRTGGNDSYRVHGGIEQDVARGQVTIREGADGILGGLLGLPGLGPVNL
                     TAQASGGATANAVAVSLSAGALSASGRGTLRLAERSADVDFSASAPAMRPAADIAWQS
                     LSADGHFHGNFDAPLIEAHVVVDGAESAGLGVKQLTLDLAGQGGGIDLRARAAGLTLP
                     GAPPDLFAHAPVALQAHAELTAAARPVRFTISHPLADLRGQAQTRDDVTASAVLTVPS
                     IAPFAALQNIDADGSATFRIALMQSGPRTKLALDARMDTQGKALVARLLGRGATLALN
                     ATVTGGDITDSTLELHGAAIEGKISGSLRKRVLDYRLALRLPDVSRLSGALHGTLSLQ
                     GGATGPIETAAVSIGGNADLARKGFARQRVTVNWQSAGLPMPSNARLSVSGRFDNAPL
                     AVNAVLGGVLERTAHLTARWKSLDANADFALSEGGAATGKGGITLKRLADFAAFTGSD
                     LAGSANAELRFE"
BASE COUNT          435 a          916 c          909 g          501 t
ORIGIN      
        1 caatgccacc gcgggcctgg gctataatct ttccgcgcga tacatccagc ccatgtttct
       61 ggagataaac cagacgctgg aattcgatct gtcgggcatc aaacagcaac tggatgccta
      121 tgatcagagg gcggaaacca tcggcgtgtt tgtgcggcgt aaattctcgc cgttatggag
      181 cgccggtgcc ggattgacgc tgactcacga cgacattacg caggaaggca ccaagcgcct
      241 ctatcagctt ctcgccctgc cgctcacggg aagctatgac agcacgggcg tcatcgacgg
      301 gcttcgcgat cccacaacgg ggttgcgcgc ctccctggcg ctgaccccca cgcacgcctt
      361 cggagccgcc aaccttaatt tcctccctgt ccagatatcg gcgtcatcct atttcgatat
      421 cggcggcgac ggccgctcgg tgatcgcgct gcgcgccctc gccgccagca ttctcggcgg
      481 ctcccatctc gaactgccgc cggatcagag gctttatgtc ggcggcagcg ccacggtgcg
      541 cggctttgcc tatcaatcca tcgggccgca attcgccgac ggcaagccga tcggcgccaa
      601 atcggtcgat gccggaacga tcgaattccg ccagcgcatc ggcgaggatt ggggcgccgt
      661 ggcatttttc gatgccggtc aggccggtaa aggcggcacg cccttcagcg gtacgccgcg
      721 cgtcggcgcc ggcgtgggcg cgcgttatta taccccgatc ggcgccgtgc gtctggacgt
      781 ggccgtgccg ctgacgcgca tgccgcgtgg cgacgcattc gagctttata ttggactggg
      841 ccaggccttc tgatgcgcga gggacgcaac atcgtacggc gtgccgggct gtggagcctg
      901 gcgatagccg cgtgtgtcgc ggtactgctc ggcgcgttgc tttatacgcc ggcggggctg
      961 gcatggagcg ggcggcttgt ccggcccttg tcggacggca ccgtcacggt gatcggcctc
     1021 ggcggcgtct ttcccaacgc gctgcatgcc gacgccgtcg agatcgccga taccgagggc
     1081 gtttggctgc gcctcgaaca ggtgacgctg cattggtcgg cgctgccggc cttgaacaac
     1141 catatcgtcg tgcagagcgt gatcgcggcc aacattcgcg tgctgcgccg gcctttgccc
     1201 tcggagacat cgcaaggcaa aacaccgcgc atcgatatcg agcatttttc cgcgccgcat
     1261 atcgagatcg ccgcgccgct gctgggccgc agcgccgtcc tcgccgcgga gggcacactg
     1321 cattatcagt cgcgccatca actggcggcc gatatcgcgg tgacgcggac cggcggcaat
     1381 gactcgtacc gcgttcatgg cggcatcgaa caggatgtgg ccagggggca ggtcaccatc
     1441 cgcgaaggcg ccgacggaat tctgggcggc ttgctcggct tgcccggtct cggtccggtc
     1501 aatctgacgg cgcaggcgag cggcggcgct acggcaaatg ccgtcgccgt cagcctgtcg
     1561 gccggtgcgc tcagcgcctc ggggcgcggc accctccgct tggcagagcg cagcgccgac
     1621 gtcgattttt ccgcgtcggc gcccgccatg cgcccggctg ccgacatcgc atggcaatcg
     1681 ctgtcggcag acggccattt tcacggcaat ttcgatgcgc ccctgatcga ggcgcatgtc
     1741 gtcgtcgatg gcgcagagtc ggccgggctg ggcgtgaagc agctcacgct cgatctcgcc
     1801 ggccagggcg gcggcatcga tcttcgggca cgcgcggcgg gcctcacgct tcccggcgca
     1861 ccgccggatc tgtttgccca tgcgccggtc gcgctgcagg cgcatgcgga gctgacggcg
     1921 gcggcgcggc ccgtccgctt cacgatttcc catccgttgg ccgatctgcg cggccaggcg
     1981 cagacaaggg acgatgtcac ggcgtcggcg gtgcttaccg taccgtccat cgcgcctttt
     2041 gcggcattgc agaatatcga tgccgacggc agcgcaacgt tccgcatcgc ccttatgcaa
     2101 tccgggccgc gcaccaaact cgcgttggac gcaaggatgg atacccaagg caaagccctt
     2161 gttgcgcgtc ttctcgggcg cggcgccacg cttgccttga acgcgaccgt cacgggcggc
     2221 gacatcaccg attccaccct cgagctgcat ggcgccgcga tcgagggcaa gatcagcggc
     2281 agtttgcgca agcgtgtcct tgattaccgg ctggctttgc gccttcccga tgtgtcgcgt
     2341 ctgtccggcg ccctgcatgg tacgctgtcg ctgcaaggcg gtgcgacggg gccgatcgag
     2401 accgccgccg tttcgatcgg cggcaacgcc gatctggcga gaaaaggatt tgccaggcag
     2461 cgcgtgaccg tcaattggca gtccgcggga ttgccgatgc cttcaaatgc gcggttgagc
     2521 gtaagcgggc gtttcgacaa tgcaccgctt gccgtcaacg ctgtgctggg cggcgtcttg
     2581 gagcgaaccg ctcacctgac ggcgcgatgg aaatccctgg atgcgaacgc ggacttcgcc
     2641 ctgtccgaag ggggcgccgc aaccggcaag ggcggcatca ccctcaagcg gctggcggat
     2701 tttgccgcct tcacaggcag cgacctcgcc ggcagcgcga atgccgagct gcgcttcgaa
     2761 g
//
LOCUS       DASWPU010000086         6181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_12886,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000086 DASWPU010000000
VERSION     DASWPU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12886"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VGC27_01885"
     CDS             complement(<1..291)
                     /locus_tag="VGC27_01885"
                     /inference="COORDINATES: protein motif:HMM:NF022464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HEY0281354.1"
                     /translation="MLTANRLVDGDVVYWRAGSWAEAFGDGDVFATKEEAEAALEAAK
                     KSVAANEVVNPYLFEVDADGGRPLKEREFVRAAGPTVRLDVGKQASGITPHRT"
     gene            547..999
                     /locus_tag="VGC27_01890"
     CDS             547..999
                     /locus_tag="VGC27_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281355.1"
                     /translation="MKSVLFAGVCALALSCTIPASAKQWVDYSPQKGYWEVMSVKVDP
                     NKIDDYLTGLKATWVPGAEIAKKSGLIDDYEVMIKLNAADGNGNVLLCLHYTSFANLD
                     PNKKRDQALDKEFEAALPKDKADAAVAGFDKIRTFVGDDIYVPVTFTK"
     gene            complement(1102..1887)
                     /locus_tag="VGC27_01895"
     CDS             complement(1102..1887)
                     /locus_tag="VGC27_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0281356.1"
                     /translation="MSAALKYLLPLALGLAVLAGWQIAVRVMDVPVYLLPAPSDIWAA
                     FVENFPSLMAALWATLSVTLAAFLLALIGGVGLAIIFSRSRMVENALYPYAVILQVTP
                     MVAIAPLILIWIGYDRIELALILIAFLVAFFPILSNTVLGLKSADHNLIDLFRLYGAK
                     RRHILWRLQLPGALPYLLGAMKIAGGLALIGAVVAEFVAGSGTSTGLAWRIVEAGNRL
                     EIAKMFACLGLLAALGIAIFASLSALEYAMLHKWHESALRKQN"
     gene            complement(1884..2630)
                     /locus_tag="VGC27_01900"
     CDS             complement(1884..2630)
                     /locus_tag="VGC27_01900"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0281357.1"
                     /translation="MSNAAILSLKGVSKTFASGIQALAGLDLDVADGEFLSLVGPSGC
                     GKSTALRLIAGLLAPDGGTVSFAAGKPETAFVFQEPTLMPWADALTNARLALDLEGVA
                     RAEAEARAAQALVRAGLKGFERAYPRELSGGMKMRVSIARAVAARPKLLLMDEPFAAL
                     DEPARQALNDDLLTLWREDGMTIVFVTHSVYESAYLSTRVAVMRARPGRVIEEIALSP
                     PAPRDESYRLTPEFAAAARQISAALKEKAA"
     gene            complement(2736..3734)
                     /locus_tag="VGC27_01905"
     CDS             complement(2736..3734)
                     /locus_tag="VGC27_01905"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0281358.1"
                     /translation="MKFRLLFVALALSSIASAQAAPAAPLTRIVFVTDWKAQAEHGGF
                     YEAVALGLYKRAGLDVRIREGGPSVNVPQLLAGGAADFGIGSNGFVPLNMVRQGVPVR
                     AVMAVFQKDPAILMTHPRGDVRRLADIKGKPVMLGDTATVTIWPWLKAKYGFDDGQIR
                     KYTFSLQPFLIDKNAIQQGYLTSEPYTWEATAHFKPQVFLLADNGYSSYANLVLVPQR
                     WIDQKPAAVQAFYDATRDGWHHYLYGDPRPANALIKRANPDMSDAVIAQAIAKMKSYG
                     LVLSGDAKAFGIGAMTDLRWKRFFEGMSAEGLYPKTLDVRKAYDLSFMRNAPQYFE"
     gene            complement(4057..>6181)
                     /locus_tag="VGC27_01910"
     CDS             complement(4057..>6181)
                     /locus_tag="VGC27_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY0281359.1"
                     /translation="IVHLAGRMRAAMHQILEAFLRGVDDLHDWAVAHRHILTKICAWI
                     AGIIVVPVAGLFLLPYLIGLFAPKLDLSQDLYALNRPVAYTFLDATGEIVGRRGALVG
                     ERLTLDQMPAYLPAAFIAMEDRRFYSHHGIDPKGLLRALWVDLLAGQWVAGGSTISQQ
                     TAKVLFTNSERKVSRKLIDMIDAVALEKSLSKKQILELYLNRLYLGSGAYGVDGAAHV
                     YFGKSAQKLTLAEAAMLATLTRAPSVFSPRRDLGRAQQRADVVLDSMVDTGAISEAEA
                     AEARAHPAIITDRTVTEARNFYLDTAAEEAQRKASAGGRTLSGDLKVHTTLDPRLQEV
                     ARLAARKVILGKQGKTARASEAAVMVMKPDGAVVALIGGVDYTESTFNRVTQAHRQPG
                     SAFKPFVYMAALEAGLMPWDVRDDTPVNINGWTPTNYGGRSYGVMTLAEALAHSVNTV
                     TARLAQEVGLDSVIDAARRCGITSKLEANASIALGTSEVTPWELTRAYGTIAAGGIKL
                     TPYLVTEVDGADGRVLYRRTAPEQSRVIAQHVVRDLTAMMYGVMTEGTGRGASIWTHE
                     AAGKTGTTQDYHDAWFVGFTADYVAAVWVGNDDASPMRGVTGGTLPARIWREVMTAAE
                     KGLPARPLDRSPPGEPIDMEITSSGISLTTDDEAATMQQEQLLQPPEQQGEQRKGFWD
                     WLFGSGEEKRPARNSGQDDESGGWN"
BASE COUNT         1109 a         1968 c         1997 g         1107 t
ORIGIN      
        1 tgttcggtgg ggggtaatgc cgctcgcctg tttgcccaca tccagcctca ccgtcggtcc
       61 cgccgcgcgg acgaattcgc gctccttcag cgggcggccg ccgtccgcgt ccacttcgaa
      121 cagataggga ttgaccactt cattggcggc aacggatttc ttcgccgctt cgagcgccgc
      181 ttcggcttct tccttcgtgg cgaagacgtc gccgtcgccg aacgcttccg cccacgagcc
      241 ggcgcgccaa tagacgacgt cgccgtccac aagccggttc gcggtaagca tttgggacaa
      301 aaagatcgcc atacaagata agtcacacct tctggtaggg aaatcaaggc ttcacataag
      361 aaacgtgtaa ttaaatataa ttaccgtatt atttacgtat aatctgccgc ccgccttttt
      421 tgttgcttgc gggtgacaga ggcaacggcc tcgaagaagg cgcccccggc cagtcttgtc
      481 aaccataatt aaatgcattt aagttgcact tctaagttgt agtctaatgt cattatggag
      541 agacacatga aatcggttct gtttgcaggc gtttgcgcgc ttgctctgtc ttgcaccatc
      601 ccggcatccg ccaagcagtg ggtcgattac tcgccgcaga agggatactg ggaagttatg
      661 agcgtgaagg tcgatcccaa caaaatcgac gactatctca ccggcttgaa agcaacctgg
      721 gtgcccggcg cggaaatcgc caagaaaagc ggcctgatcg acgattatga ggtgatgatc
      781 aagctcaatg cggctgacgg caacggcaat gtcctgctct gcctgcacta caccagcttc
      841 gccaacctgg accccaacaa gaaacgcgat caggcgctgg acaaggaatt tgaagctgcc
      901 cttcccaagg acaaggccga cgcggcagtc gccggcttcg acaaaatccg cacctttgtc
      961 ggcgacgaca tctacgttcc ggttaccttc accaaataga catacccttg gatgcggcgg
     1021 ccggctcgca ccggccgccg ttgtcctgaa ccgtttcgat gcagccgagc gtgccgccgc
     1081 agcgctccgg caggcgagac tctagttctg cttcctcagt gcgctttcgt gccatttgtg
     1141 cagcattgcg tattccagcg ccgagaggct tgcgaagatc gcgatgccca gcgccgccag
     1201 aagcccgaga caggcgaaca tcttggcgat ttccagccgg ttgcccgcct cgacgatgcg
     1261 ccaggccagc ccggtggagg tgcccgatcc ggcgacgaac tccgccacca ccgcgccgat
     1321 cagcgccagg ccgcccgcga tcttcatcgc gccgaggaga taaggcagcg cgcccggcaa
     1381 ttgcagccgc cacaggatgt gccggcgttt ggcgccgtaa aggcggaaca ggtcgatcag
     1441 attgtggtcg gcggatttga ggcccagcac cgtgttcgac aggatcggaa agaacgccac
     1501 caggaacgca atcaggatca gcgccagctc gatgcggtca tagccgatcc agatcaggat
     1561 caagggcgcg atcgccacca tcggcgtcac ctgcaagatc accgcgtaag gatagagcgc
     1621 gttttccacc atgcggctcc ggctgaaaat aatcgccagt cccacgccgc cgatcagcgc
     1681 cagcaggaac gccgccaaag tcaccgacag ggtcgcccac agcgccgcca ttaggctggg
     1741 gaaattctcg acgaaggcgg cccagatgtc gctcggcgcc ggcagcagat agaccggcac
     1801 atccatcacg cgcaccgcga tctgccatcc cgccagcacc gcgaggccca gcgcgagagg
     1861 cagaagatat ttgagtgccg cgctcacgcc gccttctcct tcagcgcggc ggaaatctgc
     1921 cgcgccgctg cggcgaattc gggagtcagc cgatagcttt cgtcgcgcgg cgcgggaggc
     1981 gaaagcgcga tctcttcgat cacccgcccc ggccgcgccc gcatcacggc gacgcgggtg
     2041 gagagatagg cgctttcgta aaccgaatgg gtgacgaaga cgatggtcat gccgtcctcg
     2101 cgccacagcg tcagcagatc gtcgttgagc gcctggcggg ccggctcgtc gagcgcggcg
     2161 aaaggctcgt ccatcagcag cagtttgggc ctggccgcga cggcgcgggc gatggagacg
     2221 cgcatcttca tcccgccgga aagctcgcgc gggtaagcgc gttcgaagcc tttcagcccg
     2281 gcgcggacga gcgcctgcgc ggcccgcgct tcggcttcgg cgcgcgccac gccttcgagg
     2341 tcgagcgcca ggcgcgcatt ggtcagcgcg tcggcccagg gcatcagcgt cggttcctgg
     2401 aagacgaaag cggtttccgg cttgccggcg gcgaacgaca ccgtgccgcc gtccggcgcg
     2461 agcaatcccg cgatcaagcg cagcgcggtc gatttcccgc agcccgaagg tcccaccagc
     2521 gagaggaatt cgccgtcggc gacatcgaga tccaggcccg ccagcgcctg gatgcccgag
     2581 gcgaaggtct tggagactcc cttcagagag aggatggccg cattactcat caattgtcat
     2641 ggcccgcgcc cttcgacacg ccaagccgcc ctcatcctga gcttgtcgaa gggggcggct
     2701 tggctgctca ggatgcgggc cacccaggtg agactctact cgaaatattg cggcgcgttg
     2761 cgcatgaagc tgaggtcgta ggctttgcgg acatccagag tcttgggata aaggccctcg
     2821 gcgctcatgc cctcgaagaa ccgcttccag cgcaaatcgg tcatggcgcc gatgccgaac
     2881 gccttcgcat cgcccgacaa gaccagcccg taggatttca tcttggcgat cgcctgggcg
     2941 atgacggcgt cgctcatgtc gggattggcg cgcttgatca gcgcgttggc ggggcgcgga
     3001 tcgccgtaga gataatggtg ccagccgtcg cgcgtggcgt cgtagaaggc ctggacggcg
     3061 gccggcttct gatcgatcca gcgctgcggc accagcacca ggttggcgta gctcgaatag
     3121 ccgttgtcgg cgagcagaaa gacctgcggc ttgaagtgcg cggtcgcttc ccacgtgtag
     3181 ggttcgctgg tgagatagcc ttgctggatg gcgttcttgt cgatcaggaa cggttgcagg
     3241 ctgaaagtgt atttacggat ttgcccgtcg tcgaaaccgt atttcgcctt cagccagggc
     3301 cagatcgtca ccgtggccgt gtcgcccagc atgaccggct tgcctttgat gtcggcgagg
     3361 cggcgcacgt cgccgcgcgg atgggtcatc aggatggcgg ggtctttctg gaacacggcc
     3421 atcaccgcgc gcaccggcac gccttggcgc accatgttga gcggcacgaa gccgttcgaa
     3481 ccgatgccga agtcggcggc gccgcccgcg agcagttgcg gcacgttcac gctcggcccg
     3541 ccttcgcgga tgcgcacgtc gaggccggcc cgcttgtaga ggccgagcgc caccgcctcg
     3601 tagaagccgc catgctcggc ctgcgccttc cagtcggtga cgaagacgat gcgggtgagg
     3661 ggggccgccg gcgcggcctg ggccgaagcg atggaagaga gtgccagcgc gacgaaaagc
     3721 agacgaaatt tcataatcgg ttcctatcgc ctttgttatt ctgcgcgttt gccgaagaaa
     3781 ccataacata gttccaccct ccccttgagg gagggtcgaa aaattcaagc ggagcgaaga
     3841 atttttcggg gaggggtaat ggttccgtgt gacccctccc cgaaattttc ccgctcgcta
     3901 acgctcgtcg aaaaatttcg actctccctc aacccgtctt cgctgaaact tcgacgggtt
     3961 gggcgcgcag tccggcgaag ccttggcgta gccgggctca agaagagggt ggagatttag
     4021 ggatgagctt gaaaaacgaa ttgggcgcaa ccgtgtttag ttccagccgc cgctttcgtc
     4081 gtcctggccg gagttccgcg cgggcctctt ttcttcgccc gagccgaaca gccaatccca
     4141 gaagcccttg cgctgctcgc cttgttgctc cggcggctgc agcagttgct cctgttgcat
     4201 ggtcgcggct tcgtcgtcgg tcgtcagcga aatgccggaa gaggtgatct ccatgtcgat
     4261 cggctcgccc ggcggcgaac ggtcgagcgg ccgcgccggc aggccctttt ccgccgcggt
     4321 catgacctcg cgccagatcc gggcgggcag agttccgccc gtcaccccgc gcatcgggga
     4381 ggcatcgtcg ttgcccaccc acaccgcggc gacgtaatcg gcggtgaagc cgacgaacca
     4441 ggcgtcgtga taatcctggg tggtgccggt cttgccggcg gcttcatggg tccagatgga
     4501 ggcgccgcgc ccggtgcctt cggtcatcac gccgtacatc atggcggtga ggtcgcgcac
     4561 gacatgctgg gcgatgacgc gggactgctc cggcgccgtg cgccggtaaa gcaccctgcc
     4621 gtcggcgccg tccacttccg tcaccagata gggcgtcagc ttgatcccgc ccgcggcgat
     4681 ggtgccgtag gcgcgggtca gctcccaagg cgtcacttcg ctggtgccca aggcgatgga
     4741 ggcgttggcc tcgagcttgg aggtgatgcc gcagcgccgc gccgcgtcga tcaccgaatc
     4801 cagtccgact tcctgggcga gccgggcggt gacggtgttg acggaatggg ccagcgcctc
     4861 ggccagggtc atcacgccgt agctgcggcc gccgtaattg gtcggcgtcc agccgttgat
     4921 gttgaccggg gtgtcgtcgc gcacgtccca gggcatcagc cccgcttcca gcgccgccat
     4981 gtagacgaag ggcttgaagg ccgagccggg ctggcggtgg gcctgggtga cgcggttgaa
     5041 ggtgctttcc gtatagtcca cgccgccgat cagggccacg acggcgccgt ccggcttcat
     5101 caccatgacc gcggcttcgc tggcgcgcgc cgtcttgccc tgcttgccca ggatgacttt
     5161 cctcgcggcc aggcgcgcga cttcctgcag cctggggtcc agcgtcgtgt gcactttgag
     5221 gtcgccggag agcgtccgcc cgccggccga ggcttttcgc tgggcttcct ccgccgccgt
     5281 atccagatag aagttgcgcg cttccgtgac ggtacggtcg gtgatgatgg cggggtgggc
     5341 gcgcgcctcg gcggcctccg cctcgctgat cgcgccggtg tccaccatgg aatccagaac
     5401 gacgtcggcg cgctgctggg cgcggccgag atcgcgccgc ggcgagaaca ccgaaggcgc
     5461 gcgcgtcagc gtcgccagca tggcggcttc ggcgagcgtc agcttctgcg ccgacttgcc
     5521 gaaatagaca tgcgccgcgc cgtccacgcc gtaggcgccc gagccgagat agagccggtt
     5581 gagatagagt tccaggatct gcttcttgct gagcgatttc tccagcgcca ccgcgtcgat
     5641 catgtcgatc aacttgcgcg agaccttgcg ctcggaattg gtgaacagga ccttggcggt
     5701 ctgctggctg atggtggagc cgcccgccac ccactgaccg gcaagcagat cgacccacaa
     5761 ggcgcgcagc aggcccttgg ggtcgatgcc gtgatgcgaa tagaagcggc ggtcctccat
     5821 ggcgatgaag gcggccggca gataggcggg catctggtcg agcgtcagcc tttcgccgac
     5881 cagggcgccg cgccggccca cgatctcgcc cgtggcgtcg aggaaggtgt aggcgacggg
     5941 gcggttgagg gcgtagaggt cctggctgag atcgagcttg ggcgcgaaca ggccgatcag
     6001 gtaaggcaac aggaacaagc ccgccaccgg cacaacgata atcccggcga tccaggcgca
     6061 gatcttggtc aggatgtgcc tgtgagcgac ggcccagtcg tgcagatcat ccaccccgcg
     6121 caggaacgct tccaggattt ggtgcatcgc cgccctcatg cggcccgcta ggtggacgat
     6181 a
//
LOCUS       DASWPU010000087         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_74051,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000087 DASWPU010000000
VERSION     DASWPU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74051"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..670)
                     /locus_tag="VGC27_01915"
     CDS             complement(263..670)
                     /locus_tag="VGC27_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEY0281360.1"
                     /translation="MVCAPATGPTSRRTRSESFDGQTKRARKIIRYRDSYREDRRFSC
                     WATFEVFATSALVHDAVIRNLEIVSEASRHVPADLKATAPDIAWREIADFGNLLRHGY
                     EGVNDRILWETIRRDLPPLMTAVRGFLDRPDVE"
     gene            complement(612..908)
                     /locus_tag="VGC27_01920"
     CDS             complement(612..908)
                     /locus_tag="VGC27_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0281361.1"
                     /translation="MRREEAIRLLKQAEPELRARGVRSLALFGSTARDEAGPESDVDV
                     LVELDMSRRPTLIDLSGMRLYIGDTLGCETDIALRDSLRPGYRANIEADAIRIF"
     gene            complement(941..2743)
                     /gene="lepA"
                     /locus_tag="VGC27_01925"
     CDS             complement(941..2743)
                     /gene="lepA"
                     /locus_tag="VGC27_01925"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY0281362.1"
                     /translation="MTDQAHLRNFSIVAHIDHGKSTLADRLIQTTGGLSVREMKEQVL
                     DSMDIERERGITIKAQTVRLEYKARNGETYTLNLMDTPGHVDFAYEVSRCLAACEGAL
                     LVVDASQGVEAQTLANVYQAIDAGLEIVPVLNKVDLPAAEPERIRQQIEDVIGLDASG
                     AIAISAKTGLNIEEVLEAVVHRLPPPKGDAAAPLKALLIDSWYDAYLGVIVLVRIVDG
                     VLKKGQKIRMMASGAAYQVERVGVFTPKQVLLDSLSVGEVGFITAAIKEVAETHVGDT
                     ITDDRKPAAEALPGFKPAMQVVFCGLFPVDAALFEDLREALSRLHLNDASFTYETETS
                     AALGFGFRCGFLGLLHLEIIRERLEREFNLDLIATAPSVIYRIHLTDGTMIELHNPAD
                     MPDVARIAKIEEPWIRATIFVPDEYLGAVLKLCEDRRGSQVELTYAGSRAMVVYRLPL
                     NEVVFDFYDRLKSVSRGYASFDYHVEGYHEGDLVKMSILVNDEAVDALSMIVNRARAE
                     ARGRSMCEKLKDLIPRHLFKIPIQAAIGGKIVARETISALRKDVTAKCYGGDITRKRK
                     LLDKQKEGKKKMRQFGRVDIPQEAFIAALKMDND"
     gene            2864..>3684
                     /locus_tag="VGC27_01930"
     CDS             2864..>3684
                     /locus_tag="VGC27_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281363.1"
                     /translation="MPFCSRSAAFAGMLFGASLWTGAAVAGIADSISSLMNPEWAYAG
                     LLGTWTNPDADLSDIDHIVITVSGSQQLRVQTFGRCGNRICNWGTLLGHVRSDNPKSA
                     AVRSISVDYNLGFALRHITLRRVQGNALRFDVATDFTDGSARHDYETSGRLIPLGAKP
                     AASAAPAAVGTPAAVGTPAAAGAPDATGTPVAEATPVAPASDAASAAPAATDRWWNLI
                     SGSEPVAANTGGALGNCITFDTEHNYLAPDAGRWKVRDFLHTIQDFGPYRTAASRA"
BASE COUNT          692 a         1170 c         1120 g          702 t
ORIGIN      
        1 gcggtgacgg ccgataccgc gttgcggctc ggccattggt tcggcaccag cgcggaattc
       61 tggctgaatc tgcaaaagct ctacgagctt cgtctcgccg aaaacgcaaa gggcgcggag
      121 atcaggaagt tgccgaagcg caaggcggct tagttttgat caggggaatg atgtctgaaa
      181 ccgcccaagt gaaatgcaaa cggataaccc ccgcatttca tctccgtcca tcttcgccgt
      241 tgcggcaaaa accaattcca aattattcca cgtcgggccg atcgagaaat ccgcgaacgg
      301 ccgtcataag cggcggcaga tcgcgcctga tcgtctccca caaaatgcga tcattcacgc
      361 cttcataacc gtgacgcagc agattgccga aatccgcgat ttcgcgccaa gcgatgtcag
      421 gtgcagtcgc cttcaagtct gccggaacgt gacggctcgc ctccgaaacg atctccagat
      481 tacgaataac ggcgtcgtgc acaagggcgc tcgtcgcaaa gacctcgaat gtcgcccagc
      541 aagaaaatcg tcgatcttcg cgatagctgt cgcgatatcg gataatcttt cgcgcgcgct
      601 tcgtttggcc atcaaaagat tcggatcgcg tccgcctcga tgttggcccg gtagccgggg
      661 cgcagactat cgcgaagagc tatgtcggtt tcgcagccca aggtatctcc gatgtagagc
      721 ctcatacccg acaggtctat gagcgtcggc cgccgtgaca tgtccagctc gaccagcaca
      781 tccacatcgc tttctggtcc ggcctcatcc cgggcggttg aaccgaacag cgcaagcgaa
      841 cgaacgccgc gcgcccgaag ttcgggctcg gcttgcttca gcaggcgaat ggcttcttcg
      901 cgtcgcatgg gccgattgta ccacaaacgc cgcgcgcggg ttaatcgttg tccatcttca
      961 gcgcggcgat gaaggcttcc tggggaatgt ccactcggcc aaactggcgc atcttcttct
     1021 tgccctcttt ctgcttgtcg agcagcttgc gcttgcgggt gatgtcgccg ccgtagcatt
     1081 tcgcggtcac gtccttgcgc agcgccgaga tggtttcgcg ggcgacgatc ttgccgccga
     1141 tggccgcctg gatcggaatc ttgaacaggt ggcgcgggat caggtccttc agcttctcgc
     1201 acatcgagcg gccgcgcgct tccgcacgcg cccggttgac gatcatcgac agggcgtcca
     1261 ccgcctcgtc gttgaccagg atcgacatct tgaccaggtc gccttcatga tagccttcca
     1321 catgatagtc gaagctggca tagccgcggc tgaccgattt cagccggtcg tagaaatcga
     1381 acaccacttc gttcaagggc agccggtaca ccaccatggc gcggctgccg gcataagtca
     1441 gctccacctg cgagccgcgg cggtcctcgc acagtttcag cacagcgccg agatactcgt
     1501 ccggcacgaa gatcgtggcc ctgatccagg gttcttcgat cttggcgatt ctcgccacat
     1561 cgggcatatc ggccggatta tgcagctcga tcatcgtgcc gtcggtcaga tggatgcgat
     1621 agatcacgct cggcgcggtg gcgatcagat cgagattgaa ttcacgttcc agccgctcgc
     1681 gaatgatttc aaggtggagc agaccaagga aaccgcagcg gaacccgaag cccagggccg
     1741 cggaggtttc ggtctcatag gtgaagctgg cgtcgttcag atgcagcctg gacagcgctt
     1801 cgcgcaaatc ctcgaacagc gcggcgtcaa ccgggaacag gccgcagaac accacttgca
     1861 tcgccggctt gaagcccggc aacgcttctg cggcaggctt tctgtcgtcg gtaatggtgt
     1921 cgccgacatg ggtttcggcc acttccttga tcgcggcggt gatgaagccg acctcgccca
     1981 cgctcagcga atcgagcagc acctgcttgg gggtgaagac gccgacacgc tccacttggt
     2041 aggccgcgcc cgacgccatc atgcggatct tctggccctt cttcagcacg ccgtcgacaa
     2101 tgcgcaccag cacgatgacg ccgagatagg cgtcgtacca ggaatcgatc agcagcgcct
     2161 tgagcggcgc ggccgcgtcg cccttgggcg gcggcaggcg atggacgacc gcttccaaaa
     2221 cctcttcgat gttgaggccg gtcttggcgg aaatcgcaat ggcgccggag gcatccagcc
     2281 cgatcacatc ctcgatctgc tggcggatgc gttccggctc ggcggcgggc aggtccacct
     2341 tgttgaggac cggaacgatc tctaagcccg cgtcgatcgc ctgatagaca ttggcgaggg
     2401 tctgagcctc gacgccctgg ctggcgtcca ccaccagaag cgcgccctcg caggcggcca
     2461 ggcagcggct gacctcatag gcgaagtcca catggccggg cgtgtccatg agattgaggg
     2521 tgtaggtctc gccgttcctg gccttgtatt ccagccgcac ggtctgagcc ttgatggtga
     2581 tgccccgctc gcgctcgata tccatggaat cgagcacttg ctccttcatc tcgcgcacgg
     2641 agagcccgcc ggtggtctgg atcagccggt cggccaaggt cgatttgccg tggtcgatat
     2701 gggccacgat ggagaaattg cgcaggtgtg cctgatcggt catggagggc gctatgtagc
     2761 cgcgcgcccg ggccaggtcc accctgttcc ggggcggccc gaagatgtaa gttcgccgca
     2821 atcaaccccc gagccagacc gggacgaaac caggggaggt tccatgcctt tttgcagtcg
     2881 cagcgccgca ttcgcgggaa tgttgttcgg ggcgagcctt tggacgggcg cggctgtcgc
     2941 cggcatcgcg gactccatct ccagcctgat gaatcccgaa tgggcctacg ccgggctact
     3001 cggaacctgg acgaacccgg atgcggatct gtcggacatc gatcacatcg tgatcaccgt
     3061 ttccggttcg caacaattgc gcgtccagac ctttggccgt tgcggaaacc gcatctgcaa
     3121 ctggggcacc ttgctgggcc atgtccgttc cgacaatccc aagtcagcgg ccgtgcggag
     3181 catctccgtg gactataatc tcggatttgc gctgcggcac atcactcttc gccgcgtgca
     3241 gggcaatgcg ctgcgcttcg acgttgcgac ggacttcacc gacggttccg cgcggcacga
     3301 ttacgagacc agcggccggt tgattccgct cggagcaaag ccggccgcaa gcgcggcgcc
     3361 tgctgcggtc ggcacgcctg ccgcggtcgg cacgcctgcc gcggcaggcg cgcccgacgc
     3421 aaccggcacg ccggtagcgg aagccacacc ggtcgcacca gcctccgacg ccgcatctgc
     3481 ggcccctgcc gctacggatc gctggtggaa cttgatcagc ggtagcgaac ccgttgccgc
     3541 gaacactgga ggggcgctgg gcaactgcat cacgttcgat accgagcaca attatctggc
     3601 gccggatgca ggccgctgga aggtccgcga tttcttgcac acgatccagg atttcggacc
     3661 ctatcgcacc gccgcaagca gagc
//
LOCUS       DASWPU010000088         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_55795,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000088 DASWPU010000000
VERSION     DASWPU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55795"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /gene="pnp"
                     /locus_tag="VGC27_01935"
     CDS             complement(<1..1457)
                     /gene="pnp"
                     /locus_tag="VGC27_01935"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY0281364.1"
                     /translation="MFKTIREEIDWGGRKLVLETGRVARQADGAVVATYGETVVLATA
                     VGARSPKPGVDFFPLTVNYQEKFFAAGKIPGGYFKREGRPTERETLTSRLIDRPIRPL
                     FADGYRNETQVVVTVLSHDLENDPDIVGLVAASAALTISGIPFMGPIGAARVGFIDGE
                     YVLNPEIDAMPNSKLDLVVAGTASAVLMVESEAQELSESEMLGAVMFGHRQMQPVIDG
                     IIKLAEKAAKEPRDIQLDDTKEIYAKIKAFAGADLAAAFQIPEKHLRGDRIKEIRGRA
                     AAEFAGEGKLEEGKLSGLMHDLEADVMRNAVLDTKRRIDGRDLVTVRPIMAEVGVLPR
                     THGSALFTRGETQALVVATLGTGDDEQFIDSLEGTYKQNFMLHYNFPPFSVGETGRMS
                     GPGRREIGHGKLAWRAVHPMLPKKEDFPYTLRVVSEITESNGSSSMATVCGSSLALMD
                     AGVPLKRPTAGIAMGLILEDKRFAVLSDILGDEDHL"
     gene            complement(1592..1861)
                     /gene="rpsO"
                     /locus_tag="VGC27_01940"
     CDS             complement(1592..1861)
                     /gene="rpsO"
                     /locus_tag="VGC27_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY0281365.1"
                     /translation="MTITTERKKVVMSEYATKPGDTGSPEVQVAVLSERIANLTEHFK
                     SHVKDNHSRRGLIKMVSQRRRLLDYIKASDAKRYEVLIGRLGLRR"
     gene            complement(1866..>2380)
                     /locus_tag="VGC27_01945"
     CDS             complement(1866..>2380)
                     /locus_tag="VGC27_01945"
                     /inference="COORDINATES: protein motif:HMM:NF027523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281366.1"
                     /translation="EPRTVLIKSARLLETPDPDHAEFEIHCGKGTYIRAWVRDLALAL
                     GTCGHVSALRRTRVGAFCAEDAIGLETLKGFMHSPAAFEHLRPVSTALDGIPALAVTG
                     PDAVRLRSGNPILIRATQFARIAEAVPAGDDLQGLTVFCSTGEGEPVALTALQAGELR
                     PFRVFNFGTG"
BASE COUNT          412 a          762 c          759 g          447 t
ORIGIN      
        1 agatgatcct cgtcgccgag gatgtcggag agcacggcaa agcgcttgtc ctcgagaatg
       61 aggcccatgg cgatgcccgc ggtcggccgc ttcagcggca cgccggcatc catcagcgcc
      121 agcgaagagc cgcacaccgt cgccatcgat gacgaaccgt tcgactccgt gatctcggag
      181 acgacgcgca gcgtgtaggg gaagtcttcc ttcttcggca gcatcggatg gacagcgcgc
      241 caggcgagct tgccgtggcc gatctcgcgc cggcccggcc ccgacatgcg ccccgtctcg
      301 cccaccgaga agggcgggaa attgtagtgc agcatgaagt tctgcttgta ggtgccttcc
      361 aaactgtcga tgaactgttc gtcgtcgccg gtgccgagcg tggccaccac cagcgcctgg
      421 gtctcgccgc gggtgaacag cgccgagccg tgggtgcgcg gcaagacgcc cacttccgcc
      481 atgatcgggc gcacggtgac cagatcgcgc ccgtcgatgc gccgcttggt gtcgagcacc
      541 gcattgcgca tgacatcggc ttccagatcg tgcatcaggc cggagagctt gccttcttcg
      601 agcttgcctt cgccggcgaa ttccgccgcc gcacggccgc ggatttcctt gatgcggtcg
      661 ccgcgcagat gtttttcggg aatctggaac gccgcggcga gatcggcgcc ggcgaaggcc
      721 ttgatcttcg cgtagatttc cttggtgtcg tcgagctgga tgtcgcgcgg ctccttcgcc
      781 gccttttccg ccagcttgat gatgccgtcg atgaccggct gcatctggcg gtggccgaac
      841 atcaccgcgc cgagcatttc ggactccgaa agctcctgcg cttcggattc caccatcagc
      901 acggcgctgg cggtgcccgc gaccacgagg tcgagcttgg aattcggcat cgcgtcgatt
      961 tccggattga gcacatattc gccgtcgatg aagccgacgc gcgccgctcc gatcgggccc
     1021 atgaagggaa tcccggagat ggtcagcgcg gcggaggcgg cgaccagtcc gacgatgtcg
     1081 ggatcgttct ccagatcgtg gctgagcacg gtgacgacca cctgggtctc gttcctatag
     1141 ccgtcggcga acagcggacg gatgggacgg tcgatcaggc gggaggtcag cgtttcgcgc
     1201 tcggtgggcc tgccttcgcg tttgaaatag ccgcccggaa tcttgccggc ggcaaagaac
     1261 ttttcctgat agttgacggt gagggggaag aaatcgacgc cgggcttggg cgagcgtgcg
     1321 cccacggccg tggccagcac cacggtttcg ccataggtgg cgaccacggc gccgtcggcc
     1381 tgacgggcca cgcggccggt ttcgagcacg agcttgcgcc cgccccagtc gatttcctcg
     1441 cgaattgttt tgaacatttt cttgttttcc ttcgttcccg caggccggat tgccggcggg
     1501 gtttgcttga gtcatctcaa gtgcgcgcgc gtccgcggac catccgcgaa cccatgcgaa
     1561 gctttaacga gagcaaaggc gggacgcgct ctcagcgcct caatcccagc cggccgatca
     1621 gcacttcata gcgcttcgcg tcagaggctt tgatgtaatc gagcagccga cggcgctgcg
     1681 acaccatctt gatcaagccg cggcgggaat gattgtcctt cacgtgcgac ttgaaatgct
     1741 cggtcagatt ggcgatgcgc tcggagagaa cggccacctg gacttcgggc gaacccgtgt
     1801 cgcccggctt ggtggcgtat tcgctcataa caactttctt gcgttcggtc gtaatcgtca
     1861 tgcggtcatc cagttccaaa gttgaaaaca cggaacggtc tcaactctcc ggcttgcagc
     1921 gcggtcaacg cgaccggttc gccttcgccc gtagagcaga agaccgtgag cccctgaaga
     1981 tcgtcgcctg cgggaacggc ctccgcgatc cgggcgaact gggtcgcccg gatcaggatc
     2041 ggattgccgc ttctcaggcg aaccgcatcc gggcccgtga cggccaacgc cgggatgccg
     2101 tccagcgcgg tcgaaacagg cctcaagtgc tcaaaagcgg ccggactatg cataaaaccc
     2161 ttgagcgttt ccagcccaat tgcatcctcg gcgcaaaagg ccccaacccg ggttcggcgc
     2221 agggccgaaa catggccgca ggtgcccagc gccagcgcca ggtcccgcac ccaggccctg
     2281 atgtaagtgc ctttgccgca atggatttcg aactcggcat ggtccggatc cggggtctcc
     2341 agaagcctgg ccgacttgat gagcaccgtg cgcggctcca
//
LOCUS       DASWPU010000089         6696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_49501,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000089 DASWPU010000000
VERSION     DASWPU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49501"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..1251
                     /locus_tag="VGC27_01950"
     CDS             331..1251
                     /locus_tag="VGC27_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0281367.1"
                     /translation="MKDRFWRLLCCLALIGIAQASSGPKVDAILPSPAIHDAFFMTDD
                     RIRIHYLVAGRMTAKPPIVFIPGWRLTASLWNRQMQVFSRDRLVIAIDSRSQGDSSIA
                     LFGNTPERRAIDLHQLIASRRITKFSIVAWSQGVQDTAAYIERYGTTSLATITLVDSP
                     ISAGPEELTVHPGFSKAVLSRIEQYDLDPGTSTERSVRSVFGKSYPDPFIRHVIDKAS
                     KTPPSIAIAMLTADIFGIDRRSVVNKIDRPTLVIASSTSPLLDAQKDMAAAIPSARWI
                     VVPEAGHALFIDEPDIFDRELTALLEAARA"
     gene            complement(1988..3517)
                     /locus_tag="VGC27_01955"
     CDS             complement(1988..3517)
                     /locus_tag="VGC27_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY0281368.1"
                     /translation="MSYRSLREFIAKLEDAGELVRVREPVSTVLEMTEIQTRLIAQGG
                     PAVLFEKPVKADGTPSDMPVLVNLFGTVKRVAMGVTLCGVERTTAVELREVGELLAAL
                     RQPEPPRNLGEAFDYLPMAKTVMAMRPKTVSNPPCQQIVLKGGDIDLARLPVQTCWPG
                     EPAPLITWPLVVTKGPGEAREDNFNLGIYRNQVLNGTQTLMRWLRHRGGAQHHARWAK
                     ENSAPLPAAIVLGADPGIILAAVTPIPDTLSEYQFAGLLRGSRVELADCLTQPLMVPA
                     EAEIVLEGEVSLTDYRDEGPYGDHTGYYNSVERFPVFTITAITMRRDPIYLSTYTGRP
                     PDEPSILGEALNEVFVPLIRQQFPEIVDFWLPPEGCSYRIAVVSMKKAYPGHAKRVMM
                     AVWSYLRQFMYTKWVIVVDDDIDARDWKDVMWAISTRMDPARDITVIENTPIDYLDFA
                     SPESGLGSKIGLDATGKWPPEVKREWGRLIRMDEDIVRTVSEKWPRLGLPGPGKPVWP
                     R"
     gene            complement(3669..5129)
                     /locus_tag="VGC27_01960"
     CDS             complement(3669..5129)
                     /locus_tag="VGC27_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0281369.1"
                     /translation="MTEPNGAADLTQPDPVVASEIRLHRTLGPFSLIMIGIGSIVGAG
                     IFVYAGRAAAVNAGPAVLLSFAIAGTGCLFAGLCYAEFASMIPESGSSYTYAYATMGR
                     FMAWFIGWNMVLEYGVSASGVAAGWSGYFISFLQHMGVDFPAAWANAPVTGHGVHDLH
                     WTGAIVNLPAVVLIVGLTALLVVGVRESARFNNTMVLVKVGVVLMVILFGLPFVSAEH
                     FTPFIPPNTGTYGHFGISGILAGAAIAFFAFIGFEAVSVAAQECKNPQRDLPIGILGS
                     LLICSVLYILVAIVIIGIADWRTLDVPDPMSFAVGKNPNLSWLVQWVNVGAMVGLGTV
                     VFTSLYGQTRVFYSMARDGFLPPAFSNVHPRFHTPHRGTIIVGIIAALLAAIFPFDVL
                     ADLVSIGTLLAFIAVCLGIMILRVTTPKAKRKFRTPLVWLTAPAGIATCGVMMYSLSD
                     GTWARLAVWTVIGFIIYLGYGIWHAAPSKWKVKNGP"
     gene            complement(5161..6615)
                     /locus_tag="VGC27_01965"
     CDS             complement(5161..6615)
                     /locus_tag="VGC27_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0281370.1"
                     /translation="MSEITEDPVAEQREVHDLNPTLGRLHLIALGIGAIIGAGIFVIT
                     GHAAASYAGPGVMISFAIAGLGCLFAGLCYAEYASMIPVAGSAYTYTYATLGRFMAWF
                     IGWNLVLEYLAAGSTVAVGWSGYFNDFMTKQMHVAIPPEFTAAPFAVHGFHDFVSTGA
                     FFNLPAVVLIALVTTLLIVGIHLSANFNNLMVAVKLCIVVLVIAAGLPYVVGANHTPF
                     IPDNTGTFGQFGWTGVFRATGVIFFAYIGFDAVSVAAQEAKNPKRDVPIGILGSLLIC
                     TVLYMLMSYVLTGLASYTTLNVAHPVSMAVAALPGTAWLAPIVNIGAIVGLASVVLVL
                     LLGQSRIFYAMSRDGMIPPLFSNIHPTFRTPWRGSIITGIFCALLAGLLPLDILGELV
                     SIGTLLAFVIVCFGVMVLRITRPNAKRPFRTPFVWIFAPLGIGMCAFMMFFLPFDTWL
                     RLLVWTIIGTLIYFGYSVRHARKPRWHIEEHPAD"
BASE COUNT         1386 a         2035 c         2097 g         1178 t
ORIGIN      
        1 cgtcatgcgc gcgttccaag aaggacgaga ggaaaatatt tctacgaatg ccgcttaact
       61 gcatttgcaa cacgtcagcg gaccattgcg tgaaatggtc aagcttggtc ccgtgccgaa
      121 taattttgga cgtcgcacca cttccgagat gcgtcagaaa cagatgttcg gaactcccca
      181 actctgacga gtcataacac gaacttgtga cctgccaaga gcggaaaggg gcaatataac
      241 cgtgtcaaac gacatgaact gatgcctaaa cgcttcacgt ttgtccagga ttccagtcct
      301 tacgagaatt ggcgttcgga ggttcgtcga atgaaagatc gtttctggcg attgctctgc
      361 tgtcttgcac tcatcggaat tgctcaggcc agcagcggcc cgaaggtaga tgcaattctg
      421 ccgtccccgg ccattcacga tgcttttttc atgacggatg atcgcatccg cattcactat
      481 ctcgtcgccg gccggatgac ggccaaaccg ccgatcgtgt tcatccccgg ctggagactt
      541 acagcatcgc tttggaacag gcagatgcag gtcttctccc gggaccggct ggtgattgct
      601 atagactccc ggtcacaggg agattcgtct attgcactct tcggaaacac gccggaacgg
      661 cgcgccatcg atctgcacca actgatcgcc agccggcgca taacgaaatt ctcaatcgtt
      721 gcctggtcgc agggcgttca ggatactgca gcatatatcg aaagatacgg gacaacctcc
      781 ctggcgacga taaccctcgt cgatagcccg atttcagcgg gcccggagga gctcaccgtc
      841 catccgggat tcagcaaagc ggtactgtcg aggatcgaac agtacgatct cgatccgggc
      901 acatccaccg agcgctcggt tcgttcggtg ttcggcaaga gctatcccga cccgttcatt
      961 cgccatgtaa tcgataaagc cagcaagacg ccgccatcca ttgctattgc catgctcaca
     1021 gccgatatct tcggtatcga ccgcaggtcg gtcgtgaaca agatcgatcg gccgaccctt
     1081 gtgatcgcgt catcaacatc accgctcttg gatgcccaga aggacatggc ggcggcgatc
     1141 ccgtcggcgc gatggatcgt cgtgcctgaa gcgggtcatg cgttattcat cgacgaacca
     1201 gacatctttg accgcgagct tacggcgctg ctcgaggcgg cgcgcgctta gccagaattg
     1261 ggagactggg gccggagtag atgcggatat cgcgacagcg tttcgtttat tcgtaggtgt
     1321 gccatactgg gcgatggtca cagacaagct gccgtcgcac gacgcgctac tcaaagcgac
     1381 tcgatgggct acggtcgcga tcgtatcgtc tcgtgagcgg aaagcgcggt aaccaggact
     1441 cgcggcgtac ggtccagagc tcgtaggtgg gtctcagctg gtcaggcgca tccagagatc
     1501 ccaggttcac ttcgatctcg tctgctgtgc gggagaatac ggacgagccg cagcggggac
     1561 agaaaaaccg tccggcgtag tcgcgtgttt cgccttcgat cgtcaccgcc tcctgaggga
     1621 acaccgctga cgcatgaaaa agggcgccgt gatgcttgcg acagtcgagg caatgacaaa
     1681 gtccgacccg ataggggcgc cctgatgcca caattcggac gttgccgcac aagcatccgc
     1741 cggtaaatcg gtccatgctg cgcctcctcc aagatcaagc ggccggtgtg tttagagcgg
     1801 acagccccgg cattccaccc acccgctgcg cgcaggaacg cttcggcaag gtataccatt
     1861 cgcgtctctt ggcgcgacac gttcggagtt gatcgaagtt gcctgtgatt ggcacctgca
     1921 ttgggcaaca ggcgcgctgt tcacgagggg cggggcaggc ctgcgctggt gcgcgatgaa
     1981 gcgcgcgtca gcgcggccac accggcttgc ccgggccggg caggccgagg cgcggccatt
     2041 tttcgctcac cgtgcgcaca atgtcctcgt ccatgcggat gaggcggccc cattcgcgct
     2101 taacctcagg cggccatttg cccgtggcgt ccagcccgat cttggagccc agtccgctct
     2161 ccggcgaggc gaaatccaga taatcgatcg gcgtgttctc gatcaccgtg atgtcgcgcg
     2221 ccgggtccat gcgggtggaa atggcccaca tcacgtcctt ccaatcgcgc gcgtcgatgt
     2281 cgtcgtccac cacgatcacc catttggtgt acatgaactg gcgcaggtac gaccacaccg
     2341 ccatcatcac gcgcttggcg tggccgggat aagctttctt catgctgacg accgcgatgc
     2401 ggtagctgca tccttccggc ggcagccaga aatcgacgat ctcgggaaac tgctggcgga
     2461 tcagtggcac gaacacttcg ttcagcgctt cgccgagaat cgaaggctcg tcgggcggcc
     2521 ggccggtata ggtggacaga tagattggat cgcgccgcat cgtaatggcg gtgatcgtaa
     2581 agacggggaa ccgctccacc gaattgtaat agccggtgtg atcgccataa gggccttcgt
     2641 cgcgataatc ggtcagcgag acttcgccct ccagaacgat ttcggcttcg gcgggaacca
     2701 tcaatggctg cgtcaggcaa tcggcaagct cgacacggct gccgcgcaag aggccggcga
     2761 actggtattc cgacagcgta tcggggatcg gcgtcaccgc cgccaggatg atgcccggat
     2821 cggcgccgag cacgatggcc gccggtagcg gggcggagtt ttctttcgcc cagcgggcgt
     2881 gatgctgcgc gccgccgcgg tgccgcagcc agcgcatcag cgtctgggtg ccgttcagca
     2941 cctgattgcg gtagatgccg agattgaaat tgtcttcgcg cgcctcgccc ggccctttgg
     3001 tcacgaccag tggccaggtg atcagcggcg ccggttcgcc gggccagcag gtctggaccg
     3061 gcagccgcgc aagatcgatg tcgccgccct tgagcacgat ctgctggcag ggcggattgg
     3121 agacggtctt gggccgcatc gccatgacgg tcttggccat gggcaaatag tcaaacgctt
     3181 cgccgagatt gcgcggcggt tccggctggc gcagcgcggc cagcaattcg ccgacttcgc
     3241 gcagttccac ggccgtagtg cgttccaccc cgcacagcgt cacgcccatg gcgacgcgct
     3301 tgacggtgcc gaacaggttg accagcaccg gcatgtcgct cggcgtgccg tcggccttga
     3361 ccggcttttc gaacagcacc gccggcccgc cctgcgcgat cagccgcgtc tggatttcgg
     3421 tcatttcgag cacggtcgag accggctcgc gcacgcgcac cagttcgccg gcgtcttcca
     3481 gcttggcgat gaattcacgc agcgaacgat acgacatatc cgtcatcccc ggcgagccgc
     3541 gtcgaagctg caaagcaacg aagacgggcg agggaagggg acccaggtcc aagagagctt
     3601 catggcttgc cttccccttg cgacattagc atgtcgcccg gccgggaatg acatggcctc
     3661 ctcatttctc agggtccgtt tttgactttc cacttggacg gcgcggcgtg ccagatgccg
     3721 tagccgagat atatgataaa cccgatcacg gtccaaacgg ccagcctggc ccaggtgccg
     3781 tccgagagcg aatacatcat cacgccgcag gtggcgattc cggccggtgc cgtgagccac
     3841 accaaggggg tgcggaactt gcgtttggcc ttcggcgtgg tgacgcgcag gatcatgatt
     3901 cccaagcaga ccgcgatgaa ggcaagcagc gtgccgatgg acacgaggtc ggccagcacg
     3961 tcgaagggga agatggcggc cagcagcgcg gcgatgatgc cgacgatgat ggtgccgcga
     4021 tggggcgtgt ggaagcgcgg atgcacgttg gaaaaggcgg ggggcaggaa gccgtcgcgc
     4081 gccatgctgt agaagacgcg cgtctggccg tagagcgaag tgaacaccac ggttcccagc
     4141 cccaccatgg cgccgacatt tacccattgc accagccagg acaggttcgg gtttttgccc
     4201 acggcgaagc tcatcggatc gggcacgtcc aatgtgcgcc agtcggcgat gccgattatc
     4261 acgatcgcca ccaggatgta gagcaccgaa cagatcaaca gcgagccgag aatgccgatg
     4321 ggcaggtcgc gctgcggatt cttgcactcc tgcgcggcga ccgaaaccgc ctcgaagccg
     4381 atgaaggcga agaaggcgat ggccgccccg gccaagatgc cggaaatgcc gaaatgcccg
     4441 taagtacccg tgttgggcgg aatgaaggga gtgaaatgct cggcgctcac gaagggcagg
     4501 ccgaacagga tcaccatcag cactacgccg accttcacca aaaccatcgt gttgttgaag
     4561 cgcgccgatt cccgcacgcc caccaccagc agcgccgtca gcccgacgat cagcaccacg
     4621 gcgggcaggt tgacgatggc ccccgtccaa tgaagatcgt gcacaccgtg gccggtgacg
     4681 ggcgcattgg cccaggcggc cgggaaatcc acccccatat gttgcagaaa gctgatgaaa
     4741 tagcccgacc agcccgccgc cacgcccgag gccgaaacgc cgtattccag caccatgttc
     4801 cagccgatga accaggccat gaagcggccc atggtggcat aggcataggt gtaggaactg
     4861 cccgactccg ggatcatcga ggcgaattcg gcgtagcaca ggccggcgaa cagacagccc
     4921 gtgcccgcga tggcgaagga gagcagaacg gcgggtccgg cgttcaccgc cgccgcccgc
     4981 ccggcataga cgaagatgcc ggcgccgacg atcgagccga tgccgatcat gatcaggctg
     5041 aacggcccca gcgtgcgatg caggcggatt tcgctggcga cgacaggatc gggctgcgtc
     5101 agatctgcag ccccgttcgg ttcggtcata cgtcggcccc ttctggcatg ctttcgtgtt
     5161 tcaatcggcc ggatgttctt cgatgtgcca gcgcggtttt ctggcatggc ggacgctgta
     5221 gccgaaatag atcagcgtgc cgatgatcgt ccacaccaaa agccgcagcc aggtgtcgaa
     5281 cggcagaaag aacatcatga aggcacacat tccgatcccg agcggggcga agatccagac
     5341 gaagggcgta cggaacggcc gcttggcgtt gggccgagtg atgcgcagca ccatcacgcc
     5401 gaagcagacg atcacgaacg ccagcaacgt gccgatggaa accagttcgc ccagaatatc
     5461 cagcggcaac aagccggcca gcagcgcgca gaagatgccg gtgatgatgg agccgcgcca
     5521 gggcgtcctg aacgtcggat ggatgttgct gaacagcggc gggatcatgc cgtcccgcga
     5581 catggcgtaa aaaatccgcg attgaccgag cagcagcacc agtacgacgg aggccagacc
     5641 gacgatggcg ccgatgttga cgatgggcgc gagccacgcc gttcccggca gcgcggccac
     5701 cgccatcgac accggatgcg ccacgttcag ggtcgtgtag gaggcgagcc cggtgagcac
     5761 gtacgacatc agcatgtaga gcacggtgca gataaggagc gagccgagaa tgccgatcgg
     5821 aacgtcgcgc ttcgggtttt tcgcttcttg cgccgccacc gataccgcgt cgaagccgat
     5881 ataggcgaag aaaatcacgc cggtggcgcg gaacacgccg gtccagccga attgtccgaa
     5941 ggtgccggtg ttgtcgggaa tgaacggcgt gtgattcgct cctacgacgt agggcaggcc
     6001 cgccgcgatc accagcacca cgatgcacag tttgaccgcc accatcagat tgttgaaatt
     6061 cgccgacaga tggatgccga cgatcagcag ggtggtcacc agcgcaatca gcacgaccgc
     6121 gggcaggttg aaaaaggcac cggtcgaaac gaagtcgtga aagccgtgca cggcgaacgg
     6181 cgcggcggtg aactccggcg ggatcgcgac gtgcatctgc tttgtcatga aatcgttgaa
     6241 ataacccgac cagccgacgg ccaccgtgga gccggccgcc agatattcca gcaccaggtt
     6301 ccagccgatg aaccaggcca tgaagcggcc gagcgtcgca taggtatagg tgtaggcgct
     6361 gcccgccacc gggatcatcg aggcgtattc ggcgtagcac agtcccgcga acaggcagcc
     6421 caagccggcg atggcgaagg aaatcatcac gccggggccg gcgtaactcg ccgccgcatg
     6481 gccggtgatg acgaagatgc ccgcgccgat gatcgcgccg atgccgagcg cgatgaggtg
     6541 aagccgcccc agcgtgggat tcaggtcgtg gacctcacgt tgttcggcaa cagggtcttc
     6601 ggtgatttct gacattttag tgcccccatt attgctggcc tgttgtgcag gccttaaaag
     6661 tgggcactta cgagccttgc gcccgcgcct aacgta
//
LOCUS       DASWPU010000090         7919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_74054,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000090 DASWPU010000000
VERSION     DASWPU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74054"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..444
                     /locus_tag="VGC27_01970"
     tRNA            371..444
                     /locus_tag="VGC27_01970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:Gly,seq:ccc)
     gene            498..1457
                     /locus_tag="VGC27_01975"
     CDS             498..1457
                     /locus_tag="VGC27_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY0281371.1"
                     /translation="MTKPKQPDSRKKPVPPEANKREPEPWQIPKHPAEDPDAPARIKA
                     IQDNPSSRRSDLDLEFLAEYPVRGIRLQIDYLKPELLLEAHGIRNTIVVFGSTRICEP
                     AAAHRTAATLKAASTDDPEDKDLAHRLAVAERILAKSHYYDVAREFGRLASRANQTAE
                     GPHCTVVTGGGPGIMEAANRGAFDAGVETVGFNISLPHEQYPNPYITPGLCFNFRYFA
                     MRKLHFLLRAKALVAFPGGFGTFDELFEAITLVQTGKVKPMPIVLVGESYWRRAVDMD
                     FLVEEGVIAQKDRGLFWFAETAREIWDGILHWYDKSGVPLSAF"
     gene            1484..2869
                     /locus_tag="VGC27_01980"
     CDS             1484..2869
                     /locus_tag="VGC27_01980"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0281372.1"
                     /translation="MKLSFHGADRGVTGSCHLVECAGKRILIDCGLFQGGRELEEENE
                     EPFGFDPAGIDYVLLTHAHLDHCGRLPLLVKRGFRGEIIATAATAELARLVMVDSAHL
                     QEEDARWRAKKAKNHKKERPIAPLYSIIDALNSVGRFGRAAVYGKPIKLAKGIGVTFV
                     NAGHILGSASVLLELAEKTHRTRVLFSGDLGKASDPLLVGPEKPPQADHVVMETTYGN
                     RNHKPLQPSIEEFYEAVTATFQRGGNVVVPTFAVERAQELLHVISDGIEDSRLPPSTQ
                     VYMDSPMAISATEIMQRYLDELQPEIAEHIRDGRDPFRFDGLHMTREHAESMVLNDIE
                     SGAIIMAGSGMCTGGRVRHHLRHNLPRAESSVVFVGFAANGTLARRIVDGAKEVTIFG
                     KRIPVRARIHTINGFSAHADQQQLLAWHKQTAAARTFLVHGEASTMRIFAECLGDARV
                     EMPEMNQAYEL"
     gene            3076..4242
                     /locus_tag="VGC27_01985"
     CDS             3076..4242
                     /locus_tag="VGC27_01985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY0281373.1"
                     /translation="MLNASNKKPAITPAENEKLFRKLVDAVQDYAIFMLDTEGNVLTW
                     NSGAERLKGYAAAEIIGRHYSCFFLSDAIAAGLPAKQLRQARINGRFQEEGWRMRKDG
                     STFWADIVLAPIYSEEKNLLIGFAKITRDLTARKQQQEALKSAMIAAEAADRAKSAFL
                     ANISHELRTPLNAIIGFSDMMQSEMFGPIGAQYRGFANHILVSGKRLLELINDVIDLS
                     NLEAGYSVVQEEDVDLEEVVRDSILSLRDLAESKQIALTATLETTPGKLRADRKKLRQ
                     IITNLLSNAVKFTPASGRVTISTASRGKALALTVTDTGIGMSPEEIPVALSQFGQVDS
                     SHSRKYEGTGLGLPIVKQLAALHNAEVAISSTLGKGTAVTVLFPAERVMDTGSK"
     gene            4870..6159
                     /locus_tag="VGC27_01990"
     CDS             4870..6159
                     /locus_tag="VGC27_01990"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY0281374.1"
                     /translation="MANVAVIGAQWGDEGKGKIVDWLSARADVVVRFQGGHNAGHTLV
                     IDGVTYKLSLLPSGVVRPGKFSVIGNGVVVDPYALAAEIDKLRGLGVSVTPESLIVAE
                     NAALILSLHRELDVVRESGTVQALGTTKRGIGPAYEDKVGRRAIRAIDLRNPDTLPAK
                     IARLLAHHNALRRGTGVAELTVEAVFKELMEIAPKVLPFVGPVWRKLDEARHTCKRVL
                     FEGAQAVLLDVDHGTYPFVTSSNTVAGQAAAGSGISPRQIGYVLGIAKAYTTRVGEGP
                     FPTEQKNEIGERIGSRGAEFGTVTGRKRRCGWFDAVLVRQTVVTGGIDGIALTKLDVL
                     DGFDEIKVCVGYKLGAADRDYLPADAVEQAALEPVYETIPGWSESTHGARSWAELPAN
                     AIKYVRRVEELIGAPVALLSTSPEREDTILVHDPFLG"
     gene            6249..6620
                     /locus_tag="VGC27_01995"
     CDS             6249..6620
                     /locus_tag="VGC27_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY0281375.1"
                     /translation="MYNCSKCPGYCCSYHTIPVTRRDLKRLAAHFGIAFSKAKVKFVV
                     KIAEKDKKNGEFKMRRKADEYFGRICRFFDTEERRCTVYKARPAICRGYPSGRCGYYD
                     FLCNERSTQDDPELIALTWNK"
     gene            complement(6630..7433)
                     /gene="thiD"
                     /locus_tag="VGC27_02000"
     CDS             complement(6630..7433)
                     /gene="thiD"
                     /locus_tag="VGC27_02000"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY0281376.1"
                     /translation="MTVAVKPARILIVAGSDSSGGAGIQAGIKTASAFGAYAMTAITA
                     VTVQNTKGVEAIHAVPAAIVRGQIVAALSDIGADAIVVGMLFSASIVRAVAETLAAQA
                     GGIPIVVDPVMVSTSGAALLDPGAVEALKKRLLPLATLVTPNIPEAKRLTGIGGTSPR
                     DIRRAAARLREMGAKAALIKGGHSTRSTIDDLLVWEMGEDVLAFPRIETRHTHGTGCT
                     LAAAIACGLGQGLSLALAVSRAREYVQSAIATAPGFGHGHGPLNHAAKP"
     gene            complement(7430..>7919)
                     /locus_tag="VGC27_02005"
     CDS             complement(7430..>7919)
                     /locus_tag="VGC27_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281377.1"
                     /translation="VMSNAGLDRYLESLDLKLARAQVGDRYVIEEMKKGLYNVGGEQS
                     GHIVLSDFCTTGDGLIAALQVLAVIAQEGKTASQAAHLFEPLPQVLKNVRFGKGAPLE
                     DARVKSCIEDCEARLNGGGRILVRKSGTEPVIRVMAEGDDETLIQAVVHDIACAIEEA
                     AA"
BASE COUNT         1529 a         2536 c         2469 g         1385 t
ORIGIN      
        1 gccttgattg tcaatcatct gcgaaggcgg cgtgcatgcg cctctgcata atcatcggcc
       61 aaggcctgcc gtacacttac tcagatggca ccattcgcca cgctccgcgc cgcgtgcggt
      121 gcccaacttc tgaacagaaa atcctcggga taaattgacg cggtgccggc ttgctcgttg
      181 gcgcaggctt taaggcgttc ccggcggcag atcgatacgg ccgcccgcgc aattgcggcg
      241 caatgcccga cgatgcatag tcccggcgct tgggtcgtgg ggcatttgtc cctatcttgg
      301 gcacagtagg gccaaaaaag agtcaaacgg atttcacggc ttctcgtaaa tccttgaaac
      361 aaaaggttaa gcgggcgtag ttcaatggta gaacggcagc ttcccaagct gcatacgagg
      421 gttcgattcc cttcgcccgc tccagcactc ggccgaacgc gaacggtgct taactcaacc
      481 ttcgacggcg caacatcatg acaaaaccaa agcagccaga ttcccgcaag aaaccggttc
      541 cgccggaagc gaacaaacgg gaaccggaac cgtggcaaat ccccaagcat cccgcggaag
      601 acccggacgc tcccgcgcgc atcaaggcga tacaggataa cccttccagc cgccgctccg
      661 acttggattt ggaattcctg gcagagtatc ccgtaagagg gattcggttg cagatcgatt
      721 atctgaagcc cgaacttctg ctggaagcgc acggcatccg caatacgatc gtggtgttcg
      781 gcagcacgcg gatttgcgag cctgccgcgg cgcatcggac ggcggccacc ttgaaggcgg
      841 cgtcgacgga cgaccctgaa gacaaggact tggcgcaccg cttggccgtg gccgagcgca
      901 ttctcgcaaa gagccattat tacgacgtgg cgcgcgaatt cggccgtctg gcgagcaggg
      961 caaaccaaac cgccgagggg ccgcactgta cggtcgtgac gggcggcgga cctggcatca
     1021 tggaagccgc caatcgcggc gccttcgacg cgggtgtcga gaccgtcggg ttcaacatca
     1081 gcctgccgca cgagcagtac cccaatccct acatcacgcc cgggctctgc ttcaactttc
     1141 gttacttcgc gatgcgcaag ctccactttc tgttgagggc caaggcgctg gtcgcttttc
     1201 cgggaggatt cggcaccttc gacgagctgt tcgaggccat tacgctcgtc cagacgggca
     1261 aggtgaaacc gatgccgatc gtcctggtcg gcgaatccta ttggcgccgc gccgtggaca
     1321 tggattttct ggtcgaggaa ggcgtcatcg cccagaaaga ccggggattg ttctggttcg
     1381 ccgaaacggc ccgggagatc tgggacggca tcctgcattg gtacgacaag agcggcgtgc
     1441 cattaagcgc tttctaaggg ttcggccaac gggagtattt gccttgaaac tgtcgttcca
     1501 cggcgccgac cgcggcgtca ccggttcgtg ccacttggtc gagtgcgccg gaaaacgcat
     1561 cctgatcgat tgcgggctct tccagggcgg ccgcgagctt gaggaggaga acgaagagcc
     1621 gttcggcttc gatccggccg gcatcgacta tgtcctcttg acccatgccc atctcgatca
     1681 ctgcggccgg ctgccgctgc tcgtcaagcg cgggtttcgc ggcgagatca ttgcgaccgc
     1741 ggcgaccgcg gagctggccc ggctggtgat ggtcgattcg gcccatcttc aggaagaaga
     1801 cgcccgctgg cgcgcgaaga aggccaagaa ccataaaaag gaacggccga tagcccctct
     1861 ctactcgatc atcgatgcat tgaactccgt cggccggttc ggacgagcgg ccgtttacgg
     1921 caagccgatc aagctcgcga agggcatcgg cgtgaccttc gtcaatgccg gccacatcct
     1981 ggggtccgcc agcgtcctgc tggaacttgc ggaaaagacg caccggacgc gcgtgctgtt
     2041 ctcgggcgat ctcggcaagg caagcgaccc gctgctcgtc ggccccgaaa aaccgccgca
     2101 ggccgaccat gtggtgatgg agacgaccta cggcaaccgc aatcacaagc ctttgcagcc
     2161 gtccatcgag gagttctacg aggccgtcac ggccacgttc cagcgcggcg gcaacgtcgt
     2221 ggttccgacc ttcgcggtgg agcgggcgca ggaattgctc cacgtcatca gcgacggcat
     2281 cgaggacagc cggctgccgc cctcgacgca ggtctatatg gattcgccga tggcgatctc
     2341 cgccactgaa atcatgcaac ggtatctgga cgaattgcag ccggaaatcg ccgagcatat
     2401 ccgggacggg cgcgatccgt tccgtttcga cggactgcac atgacgcgcg agcacgccga
     2461 gtccatggtg ctgaacgaca tcgaaagcgg cgccatcatc atggccggtt cgggcatgtg
     2521 cacgggcggg cgcgtgcgcc atcacctcag gcacaacctg ccgcgcgcgg aatcgagcgt
     2581 cgtgttcgtc ggcttcgccg caaacggcac gctggcgcgc cgcatcgtcg acggcgccaa
     2641 ggaggtgacg atattcggca aacgcatccc ggtgcgcgcg cgcatccaca ccatcaacgg
     2701 cttttccgcc cacgcggacc aacagcaatt gctggcctgg cataagcaaa ccgccgccgc
     2761 ccgcaccttt ctggtacacg gcgaagcatc gacgatgcgc attttcgcgg aatgtctggg
     2821 cgacgcccgc gttgaaatgc cggagatgaa tcaggcgtac gagctgtagc ggacttcacg
     2881 ggccgcatcc gagggtttgc cgcaccgata ttcgagccat cccgcctgcc tctcatgctt
     2941 catcgctacg cggtgaaaaa tccggcgcat tccgcggcag tctagggtta atcatcgagc
     3001 ggcgttcgca agaaaaactc aagaatactt aagagtttta acggagccat aactacgccg
     3061 tcggtaaatt gagcaatgct caatgcgtca aacaaaaagc ccgccatcac gccggccgag
     3121 aacgagaagc tgttccggaa gctcgtcgac gccgttcaag actatgccat cttcatgctc
     3181 gataccgaag gcaacgtcct gacctggaac agcggcgccg agcgcctcaa aggttacgcc
     3241 gccgcagaga tcatcggacg gcattattcc tgcttcttcc tgtccgacgc gatcgccgcg
     3301 ggcctgcccg caaagcaatt gcgccaggct cgcatcaacg gccggttcca ggaggaaggc
     3361 tggcggatgc ggaaggacgg atcgacgttc tgggccgaca tcgtgctcgc ccccatatac
     3421 agcgaagaga aaaacctgct gatcggcttc gccaagatca cgcgcgacct tacggcccgc
     3481 aagcagcagc aggaagcgct gaagtcggcc atgatcgccg cggaagcggc ggaccgcgcc
     3541 aagtccgcat ttctcgccaa catcagtcat gagctgcgca cgccgctcaa cgccatcatc
     3601 ggcttctccg atatgatgca gtccgagatg ttcggtccga tcggcgcgca gtatcgcggc
     3661 tttgcgaacc acattctcgt cagcgggaag cgcctgctgg agctgatcaa cgatgtgatc
     3721 gatctttcga acctcgaagc gggctacagc gtcgtgcagg aggaggacgt cgacttggag
     3781 gaagtcgtac gggactccat tttgtccttg agagaccttg ccgaaagcaa acagatcgcg
     3841 ctgaccgcga cgcttgagac cacgcccgga aagctgcggg ccgaccgcaa gaagctccgg
     3901 cagatcatca ccaacctgct gtcgaacgcg gtgaagttca cgccggcttc gggaagggtg
     3961 acgatctcca cggccagccg gggaaaggct ttggcgctca ccgtgaccga taccggcatt
     4021 gggatgtcgc ccgaggaaat tcccgtcgca ttgtcgcaat tcggtcaagt cgacagcagt
     4081 cacagccgga aatacgaggg aaccggattg ggcctgccga tcgtcaagca attggcggct
     4141 cttcataatg ccgaggtcgc gatttccagc accttgggaa aagggacggc ggtgaccgtg
     4201 ctgtttccgg cggaaagagt catggatacg ggttcaaaat aggttcgcct gcgccctgca
     4261 gcggcgactc tttatccccc ttccgcatcc ccgaatttct caatcgcatc atgcggcttg
     4321 cgcgaaatac gccggactta tccggcacgt gcgcggccgg gcgtataatg ccggcatgac
     4381 gatgatgact cgaactgcgg aaacgttcct cgtttttgcg gagaggatgg cgtgctgccg
     4441 tctctctttt tcgcgacagc aattatcccg gcgggaagct ggcggcgccc ccacccccga
     4501 cgaaaaagtc gaaaacaacc tccaaggctt aatcgtggcc gttcaggcac ttggtcgaat
     4561 cggcgccctc gtggacttgc cgccttcagc ccttgcgatg gggggcaacc tcggcttgcg
     4621 cggggaacaa ttatcttgcc gaacatcgct caagccattg gttcaattgg agaaaccgcg
     4681 ctgccgcacc ccgccgaaaa agcacgacgc gccgtccacc gcccgctata agtggtcacc
     4741 agtctgcgcc gcgcagcggc ggagactgcc acgtcgaagc gcgcatcggc gcgcgaagac
     4801 gggcttcttg gttcggcgcg gctacgactt ggcaagccgt gcattggaaa cttcatccag
     4861 gtacggacaa tggcgaatgt ggcggtgatc ggcgcccaat ggggcgacga aggcaaaggc
     4921 aagatcgtcg actggctgtc ggcgcgcgcc gacgtggtgg tgcgtttcca gggcgggcac
     4981 aatgccggcc acacgctggt gatcgacggc gtcacctaca aattgagcct gttgccgtcg
     5041 ggcgtcgtgc gccccggcaa gttctcggtg atcggcaacg gcgtcgtcgt cgatccctat
     5101 gcgctcgccg ccgaaatcga caagctgcgc ggcctcggcg tttcggtgac gcctgaaagc
     5161 ctgatcgtcg ccgagaacgc cgccctgatc ctctctctgc atcgcgaact ggacgtggtg
     5221 cgcgaaagcg gcaccgtgca ggcgctgggc accaccaagc gcggcatcgg cccggcttat
     5281 gaggacaagg tggggcggcg cgccatccgc gccatcgacc tcaggaaccc ggacacgctg
     5341 ccggccaaga tcgcccgcct cctggcccat cacaacgcgc tcaggcgcgg cacgggggtc
     5401 gcggaattga ccgtcgaagc ggtgttcaag gaattgatgg agatcgcgcc gaaggtgctg
     5461 cctttcgtcg ggccggtgtg gcgcaagctc gacgaggcgc gccacacatg caagcgggtc
     5521 ctgttcgagg gcgcgcaggc ggttctgctc gacgtcgatc acggcaccta tcccttcgtc
     5581 acttcgtcca acacggtggc gggccaagcc gcggccggtt cgggcatcag cccgcggcag
     5641 atcggctatg tgctcggcat cgccaaggcc tacaccaccc gcgtcggcga agggccgttc
     5701 cccaccgaac agaagaacga gatcggcgaa cggatcggct cgcgcggcgc ggaattcgga
     5761 accgtcaccg ggcgcaagcg gcgctgcggc tggttcgacg cggtgctggt gcgccagacc
     5821 gtcgtcaccg gcggcatcga cggcatcgcg ctcaccaagc tcgacgtgct ggacggcttc
     5881 gacgagatca aggtctgcgt cggctacaag ctgggcgcgg cagaccggga ctatctgccg
     5941 gccgatgccg tcgagcaggc cgccctcgaa ccggtctacg agaccatccc cggctggagc
     6001 gaatccacgc acggcgcccg ctcctgggcc gaactgccgg ccaacgcgat caaatatgtg
     6061 cgccgcgtcg aagagctgat cggcgccccc gtggcgctgc tctccaccag ccccgaacgc
     6121 gaagacacga tcctggtgca cgaccccttc ctgggatgat gggcgaggcg attatccaca
     6181 cttgccgtgg gcctattgca gcgaattgac cgttgccgcc gtcacggcgc gcctgtatgc
     6241 agggagtcat gtataactgt tccaagtgtc cgggatattg ctgttcctac cacaccatcc
     6301 cggtgacccg gcgcgatctg aaacggctgg cggcgcattt cggcatcgcc ttcagcaagg
     6361 ccaaggtcaa gttcgtcgtc aagatcgccg agaaggacaa gaagaacggc gagttcaaga
     6421 tgcgccgcaa ggccgatgag tatttcggcc gcatctgccg cttcttcgac accgaggaac
     6481 gccgctgcac cgtctacaag gcaaggcccg cgatctgccg cggctatccg tcggggcgct
     6541 gcggctatta cgatttcctc tgcaacgagc gcagcacgca ggacgacccg gagttgattg
     6601 ccctcacctg gaacaaatag gcttggcgat tacggctttg ccgcgtgatt gagcgggccg
     6661 tggccgtggc cgaagcccgg cgccgtggcg atggcgcttt gcacatattc gcgggcgcgc
     6721 gataccgcca gcgccagcga cagaccctgc cccagcccgc aggcgatggc ggcggcaagg
     6781 gtgcagcccg tgccgtgggt gtggcgggtc tcgatccttg gaaaggcgag aacgtcttcg
     6841 cccatctccc acacgagcag atcgtctatg gtggaccgcg tcgaatggcc gcccttgatc
     6901 agcgcggcct tggcacccat ctcacgcagg cgggccgcgg cacgccggat gtcgcggggg
     6961 cttgtcccgc cgatccccgt aagccgcttc gcttcgggaa tattgggcgt caccagcgtc
     7021 gccagcggca gcaggcgctt cttgagggcc tcgaccgcgc cgggatccaa cagcgcggcg
     7081 ccgctggtgg aaaccatgac cggatcgacg acgatgggaa tgccgccggc ctgggcggcc
     7141 agcgtctcgg ccaccgcgcg gacgatggat gccgaaaaca gcattcccac gacgatcgcg
     7201 tcggcgccga tgtcggacag cgccgccacg atctgtccgc gcacgatggc ggccgggacg
     7261 gcgtggatgg cctcgacgcc tttggtgttc tgcacggtca cggcggtgat cgcggtcatg
     7321 gcgtaggcgc cgaacgccga cgccgtcttg atgccggcct ggatgccggc cccgccgctc
     7381 gaatccgagc ctgcgacgat gagaatgcgc gcgggcttca cggcaaccgt cacgccgccg
     7441 cttcctcgat ggcgcaggcg atgtcgtgaa cgaccgcttg gatcagcgtc tcgtcgtcgc
     7501 cttccgccat gacccggatg acgggttcgg tgccggattt gcgcaccagg atgcgtccgc
     7561 cgccgttcag ccgcgcctcg cagtcctcga tgcacgactt tacgcgagcg tcttcgagcg
     7621 gcgcgccttt gccgaagcgc acattcttca gcacctgcgg cagcggctcg aagagatgcg
     7681 cggcttggct tgccgtcttg ccctcctgcg cgatcaccgc cagcacctgc aacgccgcga
     7741 tcaatccgtc gccggtggtg cagaaatcgg acagcacgat gtggccggac tgttcgccgc
     7801 ctacgttata aaggcccttc ttcatctcct cgatgacgta acggtcgccg acttgcgcgc
     7861 gcgccagttt gagatccagc gattcgagat agcggtccaa cccggcgttc gacatcacc
//
LOCUS       DASWPU010000091         7424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_68080,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000091 DASWPU010000000
VERSION     DASWPU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68080"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VGC27_02010"
     CDS             complement(<1..335)
                     /locus_tag="VGC27_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281378.1"
                     /translation="MSRRKKGTPVHGWVVLDKPQGVTSTQAVAIVKRVFDAQKAGHAG
                     TLDPLATGVLAVALGEATKTVSFAMDGEKTYRFTASWGEGRDSDDAEGAVTATSAKRP
                     SREEIEAALP"
     gene            complement(325..738)
                     /gene="rbfA"
                     /locus_tag="VGC27_02015"
     CDS             complement(325..738)
                     /gene="rbfA"
                     /locus_tag="VGC27_02015"
                     /inference="COORDINATES: protein motif:HMM:NF001802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY0281379.1"
                     /translation="MPRKHASNIGPSQRQLRVGETLRHALSEILIRGDIRDPDLMGVS
                     VTITQVLPSGDMRHATAYCEPLGGKNADKIVAALNRHKAYLRGQMGHLIALKFTPELR
                     FVEDKSFAEAERIETILKSERVRRDLAKKSGGDEP"
     gene            complement(838..2817)
                     /gene="infB"
                     /locus_tag="VGC27_02020"
     CDS             complement(838..2817)
                     /gene="infB"
                     /locus_tag="VGC27_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY0281380.1"
                     /translation="MVAEEEEEAVTKKGVKTPVKAPAAKKGEDNRRRGKLTVAKALSG
                     DDERMRSIASYRRHLQRVNKGQQAPPAGPVGPREVTIPETITVAELANRMARRSVDVI
                     KVLMKNGMMATVNDVIDADTAELVAAEYGHTVKRVADSDVLEGLKGGSDDDANLKPRP
                     PVVTVMGHVDHGKTSLLDALRKTDVVAGEAGGITQHIGAYQVQLKTGQKITFLDTPGH
                     AAFTAMRARGAKVTDLVILVVAADDGVMPQTVEAIAHAKAAQVPLIVAINKIDMGGAD
                     PMKVKTELLQHEIQVEDLGGQTIAVEVSAKKGTGLDRLTDMILLQAELLDLKANPDRS
                     AEGAIIEAKLDRGRGPVATALVQRGTLKVGDIMVAGSEWGRVRLLQNERGETVESAGP
                     AIPVEVLGLSAAPEAGDEFAVVDTEARAREITEYRARKRRETRQAAVSRQTLDQLLKT
                     REAGEKRLLPLVIKADVQGSAEAIQGALVKLGTDEVGVQVLQSGVGGITESDVILAQA
                     SGAAIIGFNVRADKQARERAKRDGVEIRYYAIIYNVVDDVKAALSGLLAPETREKFLG
                     NAEILEVFTISKVGKVAGCRVSEGVVRRGAKVRLIRDHVVIHEGDLSTLKRFKDEVRE
                     VQTGQECGMAFANYQDMQKGDVIEAFEVETIQRAL"
     gene            complement(2881..3405)
                     /locus_tag="VGC27_02025"
     CDS             complement(2881..3405)
                     /locus_tag="VGC27_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HEY0281381.1"
                     /translation="MSDTDETSARRTGRAPAKTLSLKKTETSTVKQSFSHGRTKAVVV
                     EKKRARVEPGKSADKAHAKEAQAKTAAPAATAAPAAAGGVQRAGVVLRQLTDEEKARR
                     GAALADARVHEVEARKRAEEEARNRAAEEERLKRERAAAEKRKAEEEARKAADEQARK
                     RAEEEALRRLEKKE"
     gene            complement(3402..4094)
                     /locus_tag="VGC27_02030"
     CDS             complement(3402..4094)
                     /locus_tag="VGC27_02030"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY0281382.1"
                     /translation="MTARAAKKDEGRERRDTGASRSRERRCIATGEMRPVEKLVRFVV
                     GPDGDVVPDIEAKLPGRGLWVTADAAALARAIAKNNFAKAAKASVKVAPDLALRVEKR
                     LVGRMQSDLGLARRAGQLVLGFDNVLRRLNDKLPPRLLIEARDGAADGRKKLAGSAAA
                     RGLRVETIDWLTRAELSLALGRENVIHAAVNPGPLAERLISDSARLKGFRVRPAAETT
                     AGSSPARNERDV"
     gene            complement(4091..5716)
                     /gene="nusA"
                     /locus_tag="VGC27_02035"
     CDS             complement(4091..5716)
                     /gene="nusA"
                     /locus_tag="VGC27_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY0281383.1"
                     /translation="MAAIAANRTELLQIADAVARDKSIDKAVVLGAMEEAMQRAAKAR
                     YGSENEIRVEIDPKTGETHVSRYLHVVELVDNDKIEISLAEARTRNPAAQVGDIIAET
                     LPPVDFSRIAAQAAKQVIVQKVRDAERERQFEEYKDRIGEIVHGIVKRSEFGSVVVDL
                     GRAEGVVRRDEMIPRENFRTGDRIRAYIYDVRRETRGPQIFLSRSHPQFMVRLFAQEV
                     PEIYDGIIEIRAVARDPGSRAKIAVISKDSSIDPVGACVGMRGVRVQAVVQELQGERI
                     DIIPWSPEAATFIVNALAPAEVSKVVLDEDTHRVEVVVPDEQLSLAIGRRGQNVRLAS
                     QLTGWQIDILTEAEESERRQKEFAERTKLFMETLDVDETVAQLLASEGFAEIEDVAYV
                     PLQELASVEGFDEETAQELQNRALEFIEARNKELDDKRRALGVADEVLEVEGVTPAMA
                     VALGEAEVKTLEDLAGCATDDLLGYYEQGKEKERVRVAGALENFDLGTDEANAIIMKA
                     RVKAGWIEELPEEPAEEEAGEPAEDGTAEEGSA"
     gene            complement(5747..6229)
                     /gene="rimP"
                     /locus_tag="VGC27_02040"
     CDS             complement(5747..6229)
                     /gene="rimP"
                     /locus_tag="VGC27_02040"
                     /inference="COORDINATES: protein motif:HMM:NF000932.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY0281384.1"
                     /translation="MLSQLEPIIAPVVEAAGYRLVRLRLMGGKRRTLQVMAEREDGSM
                     NVDDCIALSRALSDFLEREDPVEGDYELEVSSPGIDRPLTRLADFARWCGHEAKIELV
                     APEDGRKRLRATLLGLDGSEVVLEAAGRRLKFPFRAIAEAKLVLTDKLIQEDLKARKQ
                     "
     gene            complement(6436..6624)
                     /locus_tag="VGC27_02045"
     CDS             complement(6436..6624)
                     /locus_tag="VGC27_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281385.1"
                     /translation="MNDDQDDEKSPPMPLSDITTLLVCLAFLAGFLWLMFGPSPFAGF
                     TETAPPQEVTVTLPGKAK"
     gene            complement(6621..>7424)
                     /locus_tag="VGC27_02050"
     CDS             complement(6621..>7424)
                     /locus_tag="VGC27_02050"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HEY0281386.1"
                     /translation="NPVSRTRRLLYGRRKGPKLSPHKEELRRTLLPVLALRLEQGADP
                     RSYFSPPPGGEVDPAKRGEAGSGGGTGNCVDDVWLEIGFGSGEHLLELARANPGVGLI
                     GAEPYEAGVAKLLSKIEQEFSSPVHGGGAAEGGGGGTNIRIHEGDGRDIVEALPDASL
                     GRVFLLFPDPWPKTRHHKRRFVQTAMLDALARVMKQGAELRIASDDAAYVEWTLERLM
                     AHPDFAWTARSAADWNTRPSGWPQTRYEAKALHGPPAYLLFVRKGKMAP"
BASE COUNT         1074 a         2509 c         2394 g         1447 t
ORIGIN      
        1 ggcagggccg cctcgatctc ctcccgggag gggcgcttgg ccgaggtggc ggtgaccgcg
       61 ccttcggcgt cgtcggaatc gcgcccctcg ccccagctcg cggtgaagcg ataggtcttc
      121 tcgccgtcca tggcgaagga caccgtcttg gtcgcttcgc ccagcgccac cgccaacaca
      181 ccggtcgcca gcggatcgag ggtgccggcg tgccccgcct tctgcgcgtc gaagacgcgc
      241 ttgacgatgg cgaccgcctg ggtggaggtg acgccttgcg gcttgtcgag caccacccag
      301 ccgtgcaccg gggtgccttt tttcctacgg ctcatcgccg ccgctcttct tcgccaggtc
      361 gcgccgcacg cgctcggatt tgagaatggt ctcgatcctt tcggcttcgg cgaacgactt
      421 gtcctcgacg aagcgcaatt cgggggtgaa tttgagcgca atcagatggc ccatctggcc
      481 gcgcagatag gctttgtggc ggttgagcgc cgcgacgatc ttgtcggcgt tcttgccgcc
      541 gagcggttcg caataggcgg tggcgtggcg catatcgccg gaaggcagaa cctgggtgat
      601 ggtcaccgac acgcccataa gatcgggatc gcggatgtcg ccgcggatca gaatctccga
      661 cagcgcgtgg cgcagcgtct cgccgacgcg caactggcgc tgggagggac cgatgttcga
      721 ggcatgttta cggggcatgg gatggttccg tcccctcatg cttcagcaaa ctcagcatga
      781 ggaaaactac taggcccttc atcctgagct tgtcgaagga tgaaggccgc gatgcgctta
      841 cagcgcgcgc tggatggttt cgacctcgaa ggcttcgatg acgtcgccct tctgcatgtc
      901 ctgatagttc gcgaaggcca tgccgcattc ctggccggtc tgcacctcgc gcacctcgtc
      961 cttgaagcgc ttcaaggtgc tgagatcgcc ttcatggatc accacatggt cgcggatcag
     1021 gcgcaccttg gcgccgcggc gcaccacgcc ctcgctgacc cggcagccgg cgaccttgcc
     1081 gaccttggag atggtgaaca cttccaggat ctcggcattg ccgaggaact tctcgcgcgt
     1141 ttccggcgcc agcaggcccg acagcgccgc tttcacgtcg tccacgacgt tatagatgat
     1201 ggcgtaatag cggatttcga cgccgtcgcg cttggcccgt tcgcgcgcct gcttgtcggc
     1261 gcggacgttg aagccgatga tcgccgcgcc cgaagcctgg gccaggatca cgtcgctttc
     1321 ggtgatgccg ccgacgcccg attgcagcac ctgcacgccg acttcgtcgg taccgagctt
     1381 gacgagcgcg ccctggatgg cttcggcgga gccttgcacg tcggccttga tcaccagcgg
     1441 caacagccgc ttctcgccgg cttcgcgcgt cttcagcaat tggtcgaggg tctggcgcga
     1501 caccgccgcc tggcgggtct cgcggcgctt gcgggcgcgg tattcggtga tctcgcgggc
     1561 gcgcgcctcg gtatccacca cggcgaattc gtcgcccgct tccggcgccg ccgacagacc
     1621 gagcacttcc accgggatcg ccggaccggc gctctcgacc gtttcgccgc gttcgttctg
     1681 cagcaggcgc acgcggcccc attcggaccc cgccaccatg atgtcgccga ccttcagcgt
     1741 gccgcgctgc accagcgcgg tcgccaccgg gccgcggccg cggtcgagtt tggcttcgat
     1801 gatcgcccct tccgcgctgc ggtcgggatt ggccttgagg tcgagcaatt cggcctgcaa
     1861 caggatcatg tccgtgagcc tgtcgagacc cgtgcccttc ttggcggaga cctccacggc
     1921 gatggtctgg ccgcccagat cctccacctg gatttcgtgc tgcaacagtt ccgtcttcac
     1981 tttcatcgga tcggcgccgc ccatgtcgat cttgttgatc gcgacaatca gcggcacctg
     2041 cgccgccttg gcgtgggcga tggcttccac cgtctgcggc atgacgccgt cgtcggcggc
     2101 gacgaccagg atgacgagat cggtcacctt ggcgccgcgc gcgcgcatgg cggtgaaggc
     2161 ggcgtggccc ggcgtgtcga ggaaggtgat tttctgtccg gttttcaact gcacctgata
     2221 ggcgccgata tgctgggtga tgccgcccgc ttcgccggcg accacatcgg tcttgcgcag
     2281 cgcatcgagc agcgaggtct tgccgtgatc gacatggccc atgacggtga ccaccggcgg
     2341 gcgcggcttc aggttggcgt cgtcgtcgga accgcccttc aagccttcca gcacgtcgga
     2401 gtcggcgacg cgcttgacgg tgtggccgta ttcggcggcg accagctcgg cggtgtcggc
     2461 atcgatcacg tcgttgaccg tcgccatcat gccgttcttc atcagcacct tgatgacatc
     2521 gaccgagcgg cgcgccatgc ggttggcgag ttccgccacg gtgatggttt cgggaatcgt
     2581 cacttcgcgc ggaccgacgg ggccggcggg cggcgcttgc tggcccttgt tcacccgttg
     2641 cagatggcgg cgataggagg cgatcgaacg catgcgctcg tcgtcgccgg acagcgcctt
     2701 ggcgacggtg agcttgccgc gccggcggtt gtcctcgccc ttcttggcgg cgggcgcctt
     2761 gaccggcgtc ttgacgccct tcttggtgac ggcctcttct tcctcttcgg cgaccacgcg
     2821 gctgcggcgc ggcgcttcgg ccgccggctt ggctccgggg gcgcttgcgg cgccggcggg
     2881 ttattccttc ttttccagcc ggcgcaaggc ttcttcttcc gcgcgcttgc gcgcctgttc
     2941 gtcggccgcc ttgcgggcct cttcctcggc cttgcgtttc tcggccgccg cgcgttcgcg
     3001 cttgaggcgt tcttcctcgg cggcgcggtt gcgggcctct tcttccgcgc gcttgcgcgc
     3061 ttccacttcg tgtacgcgcg cgtcggccag agcggcgccg cggcgcgctt tctcctcgtc
     3121 ggtcaattgg cgcaaaacga cgccggcgcg ctggacgccg ccggcagcgg cgggcgcggc
     3181 ggtggcggcg ggcgcggccg tcttagcctg cgcctccttg gcgtgagcct tgtcggccga
     3241 tttgcctggc tcgacgcgcg cgcgcttctt ctcgaccaca accgccttgg tgcggccatg
     3301 gctgaagctc tgcttgacgg tggaggtctc cgtcttcttc agcgacagcg tcttggccgg
     3361 ggcacggccc gtgcggcgcg cggaggtttc gtctgtatcg ctcatacgtc tctttcgttg
     3421 cgggcagggc tcgaacctgc cgttgtttcc gccgccggtc gcacgcggaa ccccttcagt
     3481 cttgcggaat ctgagatcag ccgctccgcc aaagggccgg gattgacggc ggcatgtatc
     3541 acattctcgc gccccagcgc caagctcaat tcggcacggg tcaaccaatc gattgtttcg
     3601 acacgcaaac cgcgcgcggc ggccgatccg gcgagcttct tgcgcccgtc cgcggccccg
     3661 tcccgggcct cgatcagaag ccgtggggga agcttgtcgt tcagccggcg caagacgtta
     3721 tcgaacccaa gcacgagctg gccggcgcgc cgcgccagac ccagatcgga ctgcatgcgg
     3781 cctaccagcc gcttttccac ccgcaaagcc agatcgggcg cgaccttgac ggaagccttg
     3841 gccgccttgg cgaaattgtt cttggcgatg gcgcgcgcca gggcggcggc atcggccgtg
     3901 acccacaatc cgcggccggg cagcttggcc tcgatgtcgg gaacgacatc gccatcgggg
     3961 ccgacgacga aacgaacgag cttctccacc gggcgcattt cgcccgtggc gatgcagcgc
     4021 cgttcgcgcg agcgagaggc gccggtatcg cgccgttcgc gcccttcgtc ctttttcgcc
     4081 gcccgtgccg tcacgcgctg ccttcctccg ccgttccgtc ttcggccggt tctccggcct
     4141 cttcctcggc cggttcttcc ggcagttcct cgatccagcc cgccttgacg cgcgccttca
     4201 tgatgatggc attggcctcg tcggtgccaa gatcgaaatt ctccagcgcc cccgcaacgc
     4261 ggacgcgctc tttttctttg ccctgctcgt aatagccgag cagatcgtcg gtggcgcagc
     4321 cggccagatc ttccagcgtc ttgacctccg cttcgcccag cgcgacggcc atggccgggg
     4381 tgacgccttc cacctccagc acctcgtcgg cgacgcccag cgcgcggcgc ttgtcgtcga
     4441 gttccttgtt gcgggcttcg atgaattcca gcgcgcggtt ctgcaattcc tgtgcggtct
     4501 cctcgtcgaa gccttccacc gacgccagtt cctggagcgg cacataggcc acgtcttcaa
     4561 tttcggcgaa accttcggag gcgagcaact gggcgacggt ctcgtcgacg tcgagcgttt
     4621 ccatgaacag cttggtgcgc tcggcgaatt ccttctgccg acgctcggat tcctcggcct
     4681 cggtcaggat gtcgatctgc cagccggtga gctgggaggc gaggcgcaca ttttggccgc
     4741 gccggccgat cgccagcgac aattgttcgt ccggcaccac gacttcgacg cgatgcgtat
     4801 cctcgtcgag caccaccttc gacacttccg ccggcgccag ggcgttgacg atgaaggtcg
     4861 ccgcttccgg cgaccacggg atgatgtcga tgcgttcgcc ttgcagttcc tgcaccaccg
     4921 cctgcacgcg cacgccgcgc atgcccacgc acgcccccac cggatcgatg gaggagtctt
     4981 tcgagatcac cgcgatcttc gcccgcgagc cgggatcgcg cgccacggca cggatttcga
     5041 tgatgccgtc gtagatttcc ggcacttcct gggcgaacag gcggaccatg aactggggat
     5101 gggagcgcga gaggaaaatt tgcgggccgc gggtttcgcg gcgcacatcg tagatatagg
     5161 cgcggatgcg gtcgccggtg cggaaatttt cccgcgggat catctcgtcg cggcgcacca
     5221 caccttcggc gcggccgagg tccaccacca ccgagccgaa ctccgaacgc ttgacgatgc
     5281 cgtggacgat ctcgccgatg cggtccttgt attcctcgaa ttggcgttcg cgttcggcgt
     5341 cgcgcacctt ctgcacgatc acctgcttgg cggcctgcgc ggcgatgcgc gaaaaatcga
     5401 ccggcggcaa ggtctcggcg atgatgtcgc cgacctgggc ggcggggttg cgcgtgcgcg
     5461 cttcggccag cgaaatctcg atcttgtcgt tgtccaccag ctcgacgacg tgcagatagc
     5521 gcgagacgtg ggtctcgccg gtcttggggt cgatctcgac gcggatttcg ttctcgctgc
     5581 cgtagcgcgc cttggccgcg cgctgcatcg cctcttccat cgcgcccagc acgacggcct
     5641 tgtcgataga cttgtcgcga gcgaccgcgt cggcgatctg caagagttca gtccggtttg
     5701 cggcaatcgc agccatgtca tctgctccta aacattgtcg tcgaggtcat tgttttctcg
     5761 ctttcaaatc ttcctgaatc agtttgtcgg tcaggaccag tttggcttcc gcgatggcgc
     5821 ggaagggaaa tttgagacgc cggcccgccg cctccagcac cacctcgctg ccgtcgaggc
     5881 cgagcagcgt ggcgcgcagg cgcttgcgcc cgtcctcggg cgcgacgagt tcgatcttgg
     5941 cctcgtggcc gcaccagcgg gcgaaatcgg cgagccgggt cagcggccgg tcgatgccgg
     6001 gcgaagagac ctccagctcg taatcgccct cgaccgggtc ctcgcgttcg aggaaatccg
     6061 acagagcgcg gctgagcgcg atgcaatcgt ccacattcat gctgccgtct tcgcgttcgg
     6121 ccatcacttg cagcgtcctg cgtttgccgc ccatcaggcg gaggcgcacc agccggtagc
     6181 ccgcggcctc cacgaccggg gcgatgatcg gctccaactg cgacaacacc gtgagaatgc
     6241 cttatctcct cgtcatggcc gggcccttcg agacgccgct tcgcggctcc tcaggatgag
     6301 ggtcctgcca aatacaaagc ccttcccccg cggcggtctg cttagaccgc cacacaacag
     6361 aattctgttg tctgggatga gcgggcttgt acgccggcaa gaggcggaag gcaaggcgcg
     6421 aacgccttaa atcccctatt ttgccttccc gggaagggtc accgtaacct cttggggcgg
     6481 ggccgtttcg gtaaatccgg cgaagggcga aggcccgaac atcagccaga gaaagcccgc
     6541 caggaaggcc aggcagacca gaagggtggt gatgtcgctc aagggcatcg gtgggctttt
     6601 ttcatcgtct tggtcgtcgt tcatggcgcc attttaccct ttcgcacgaa aaggagatag
     6661 gccgggggtc cgtgcagggc cttggcctcg tagcgggtct gcggccagcc cgaaggacgg
     6721 gtgttccagt cggcggccga gcgcgccgtc caggcgaagt ccgggtgcgc catcagccgt
     6781 tccagcgtcc actccacata ggcggcatcg tcgctggcga tccgcagttc ggcgccttgt
     6841 ttcatcacgc gggccagcgc gtccagcatt gccgtctgaa cgaagcggcg cttgtgatga
     6901 cgcgtcttgg gccagggatc tggaaacaga aggaacacgc gcccgagcga ggcgtcgggc
     6961 aaggcttcga cgatgtcgcg cccgtcgcct tcgtggatac gaatgttggt gccccctccg
     7021 ccgccttcgg cggcacctcc cccgtgaacg ggggaggaaa actcttgttc gattttcgac
     7081 aacagtttcg ccacaccggc ttcataaggc tcggcgccga tcagcccgac gccagggttg
     7141 gcgcgtgcca gttcgagcag atgctcgccg ctgccgaagc ctatttcgag ccagacgtcg
     7201 tcgacgcaat tcccagtccc cccacccgat ccggcttcgc cgcgcttcgc cggatcgacc
     7261 tccccgccag ggggaggtga aaagtaggaa cgcgggtcgg caccttgctc tagccgcaag
     7321 gcgagcacgg gcagcagcgt gcgccgcaat tcttccttgt gcggagaaag cttcggcccc
     7381 ttgcgccgcc cgtacagcaa gcggcgggtc cgtgaaaccg gatt
//
LOCUS       DASWPU010000092         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_110786,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000092 DASWPU010000000
VERSION     DASWPU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110786"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VGC27_02055"
     CDS             complement(<1..282)
                     /locus_tag="VGC27_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281387.1"
                     /translation="MTAFFSDLRNTVAAGAAIAALLFVLYVSAQGFDAPSFWPFLIRW
                     LHVLCGVMWIGLLWYFNFFAAPMTPAALFWLRLSATGAIVFGIILAQLNG"
     gene            complement(355..1971)
                     /gene="secD"
                     /locus_tag="VGC27_02060"
     CDS             complement(355..1971)
                     /gene="secD"
                     /locus_tag="VGC27_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY0281388.1"
                     /translation="MLQVSVWVRALVSIIVLTGVMIALPNALPESVLAKMPSWLPHNT
                     LSLGLDLQGGSYVLLEVDLDGVMKDRVETTLGDIRRALRKARIAYADLKASGDAVSVR
                     ITEADQFDKALAALKAVNPATSGAAFSVGGRQYEVTQPGNGVIAMRMTEQYKRQTEAQ
                     IVSQSIEVVRRRIDELGTKEPSIEQQGKDRIVVQVPGLQDPTHLLAILQTTAKMTFQL
                     VDASADIVEAMKGNVPIGDELLPMDSGGQKSTLPPVVVEKRVMVDGDRLKDARQAINQ
                     QTGQVVVTFSFDSVGAREFGDVTKENVNRQFAIVLDKKVITAPVIREPILGGSGEISG
                     SFTVQSANDLAALLRAGALPAKLKPIEMRTVGAELGADSVKAGGYSAVAGLVLVALFM
                     ILRYGLFGAFANIALTLNVVLLLAILTMFGATLTLPGIAGIVLTMGMAVDANVLIYER
                     IREEQRNGRSILGSIDQGFRRAMSTIIDANATHLIAALILFELGSGPVRGFAVTLGVG
                     IITSFFTAVMVTRLIVVAWLLIRRPKKLVV"
     gene            complement(1978..2292)
                     /gene="yajC"
                     /locus_tag="VGC27_02065"
     CDS             complement(1978..2292)
                     /gene="yajC"
                     /locus_tag="VGC27_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY0281389.1"
                     /translation="MQDFFSPSNPLIMMIAVAAIFYFLVMRPQQRSAKRLREAIANLR
                     RGDTVVMSSGIIGRVAKVPQANDPEITVEIADDVQVKVLRGALTEVRAKSQPVEAKSD
                     KS"
BASE COUNT          456 a          757 c          748 g          423 t
ORIGIN      
        1 gccgttcaac tgcgccagga tgatgccgaa aacaatggcg cccgtcgccg acaggcgaag
       61 ccagaacaag gccgccggcg tcatcggcgc ggcgaagaaa ttgaaatacc acagcaggcc
      121 aatccacatc acgccgcaca gcacatgcag ccagcggatc aggaacggcc agaacgacgg
      181 cgcgtcgaac ccctgggcgg agacgtacag cacgaacagc agcgccgcga tcgcggcccc
      241 ggcggccacc gtattgcgca aatccgaaaa gaacgcggtc atggcccggc ccttcttatc
      301 gtgaatcggc aagggagctt actaagaaat tccggtcaac caaggcggaa ttgcctagac
      361 caccagcttc ttcggccggc ggatcagcag ccaggcgacc acgatcaggc gggtgaccat
      421 caccgccgtg aagaacgagg tgatgatgcc gacgcccagg gtcacggcga agccgcgcac
      481 cggtcccgag ccgagttcga acaggatcag cgcggcgatc aaatgcgtcg cgttggcgtc
      541 gatgatcgtc gacatggcgc ggcggaaccc ctggtcgatc gagccgagta tgcttcgccc
      601 gttgcgctgc tcctcgcgta ttcgttcgta gatcagcacg ttggcgtcga cggccatgcc
      661 catggtgagc acgatgccgg caatgccggg aagcgtcagc gtcgcgccga acatcgtcag
      721 gatcgccagc agcaggacga cgttcagagt cagcgcgatg tttgcgaacg cgccgaacag
      781 cccgtaccgc aggatcatga acaaggcgac caggaccagt cccgccacgg cggaatatcc
      841 gcccgctttc accgagtccg cacccagctc ggcgccgacg gtgcgcattt cgatcggctt
      901 gagtttcgcc ggcagcgcgc cggcgcgcag cagcgccgcc agatcgttgg cgctctgcac
      961 cgtgaagctg cccgagattt cgccggaacc gcccaatatg ggttcgcgga tcacgggagc
     1021 ggtgatgact ttcttgtcga gcacgatggc gaactggcgg ttgacgttct ccttggtgac
     1081 gtcgccgaat tcccgcgccc cgacgctgtc aaaactgaaa gtaacgacca cctgcccggt
     1141 ctgctggttg atcgcctgcc gggcgtcttt cagcctgtcg ccgtcgacca tcacccgctt
     1201 ctcgaccaca accggaggca acgtactttt ctggccgccg ctgtccatcg gcagaagctc
     1261 gtcgccgatg ggaacgttgc ctttcatcgc ctcaacgatg tcggccgacg catccaccag
     1321 ttggaacgtc atcttggccg ttgtctggag gatcgcaagg aggtgggttg ggtcctgcag
     1381 gcccggcacc tgtaccacga tgcggtcctt gccctgttgt tcgatgctgg gctccttggt
     1441 gcccagttcg tcgatgcgcc ggcggacgac ttcgatcgac tggctgacga tttgcgcctc
     1501 ggtttgcctc ttgtattgct cggtcatgcg catggcgatg acgccgttgc ccggctgggt
     1561 cacttcgtat tgacgcccgc cgacggaaaa tgccgcaccg gatgtcgccg gattgacggc
     1621 cttgagcgcc gccaatgcct tatcgaattg atcggcttcg gtgatgcgca ccgaaacggc
     1681 atcgcccgag gctttaaggt cggcgtaggc gatacgcgct ttgcgcaggg cgcggcggat
     1741 atcgccgagc gtcgtttcca cgcgatcctt catcacgccg tcgagatcga cttcgagcag
     1801 cacataggag ccgccctgga gatcgaggcc gaggctgagc gtgttgtggg gaagccagct
     1861 cggcatcttc gccagcaccg attcgggcag cgcgttcggc agcgcgatca tcacccctgt
     1921 aaggacaatg atggagacca gtgcacggac ccagacggaa acctgcaaca tcggcgatca
     1981 actcttgtcg gacttggctt ccacgggctg gcttttggcg cgcacctcgg tcagggcgcc
     2041 tctgagcact ttcacctgca cgtcgtcggc gatctcgacg gtgatttcgg gatcgttcgc
     2101 ctgcggcact ttggccaccc gcccgatgat cccggacgac atcaccaccg tgtcgccgcg
     2161 gcgcaggttg gcgatcgcct cacgcagccg tttggcgctc cgctgttgcg gacgcatgac
     2221 aaggaagtag aagatcgcgg ccacggcgat catcatgatc agcggattgg aaggggaaaa
     2281 gaagtcttgc atgggggaat ccggagcggt cgtttgcgga ggcgcccgaa gccgacggag
     2341 cgccaagagg cggactatag ccacgcagac tgcctttgca accc
//
LOCUS       DASWPU010000093         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_31299,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000093 DASWPU010000000
VERSION     DASWPU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31299"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VGC27_02070"
     CDS             <1..488
                     /locus_tag="VGC27_02070"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0281390.1"
                     /translation="ITSFEVVALDALESAIVKQGAAYWNALRGTRRYPARRELRPRGF
                     ASILPNTLLVRVVDGGADFEFRIVGEAQAQTYALPFAGKRLSEFAQVDTAYCYVLKGL
                     FGHVAEFGEPVAVRGNMGSAFSKVRFSYCESIFLPLGESDGAVDHLIGFSAYVPRDFS
                     N"
     gene            598..1674
                     /locus_tag="VGC27_02075"
     CDS             598..1674
                     /locus_tag="VGC27_02075"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HEY0281391.1"
                     /translation="MSDADDAAWDKAAPTLYAFEFRGTGSEYFRIWIVNLALTILTLG
                     VFSAWAKVRTWRYFYGNTFVGGHAFDYHASPWRILIGRAIAVGLFVGYNVTAAFKPLY
                     ALPWILILGFAFPWLVNSSLRFNARNTSYRNVRFNFTGRYFEALVAYVVWPLAGFATL
                     FLLMPAARRARDFFFTNNHTFGGRRFRTEFTPWEIYSIFLFGFFLFVTLAAVIAAGFW
                     ANVYILAKGHTIFSNQIVVSAAFIAILVFAYLAFLMLAPAINTMVVNLSLNNTRLDGR
                     HKVQSRISPLAVAWIAVTNGFLVLFTLGLFYPWARVRLRRYQTRKLALLLDGNIEDYA
                     SEAFATQSAVGEEIGSFFAFDFGL"
     gene            1679..>2260
                     /locus_tag="VGC27_02080"
     CDS             1679..>2260
                     /locus_tag="VGC27_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281392.1"
                     /translation="MLEGRYFFPHSARFVAARAAVTGAKTLRIESLDGTVLAEVPMRK
                     VRVAARLGRIARRFELPDGARYETDDNDGADDLLREARRAPGWVDRLERSWRSVLLSV
                     LLAAGAAYAFVLYGIPAIAAELALHTPSGIARVMADQTIVALDGVYLKPSKLAAGDRK
                     KAQALFARVAAHAPRGKDGYRLLLRGGGVIGPNA"
BASE COUNT          349 a          753 c          692 g          466 t
ORIGIN      
        1 acatcacctc gttcgaggtg gtcgcgctgg acgcgcttga aagtgctatc gtcaagcaag
       61 gcgccgctta ctggaacgcc ctgcgcggga ccaggcgtta tccggcccgc agggagttgc
      121 ggccgcgcgg gtttgcctcg atcctgccca ataccttgct tgtgagggtc gtcgacggag
      181 gcgccgactt cgagtttcgc atcgtgggcg aagcacaggc acaaacctac gccttgccgt
      241 tcgcgggcaa acgcctcagc gaattcgcgc aggtcgatac cgcatattgt tacgttctga
      301 agggtttatt cggccacgtc gccgaattcg gcgagccggt tgccgtgcgg ggaaacatgg
      361 ggagcgcatt ttccaaagtg cgcttctcct attgcgagag catctttctg ccgctcggcg
      421 aaagcgacgg cgcggtggat cacctgatcg gattcagcgc ctacgttccg cgggatttct
      481 ccaattaaga aatatgaccg tcttgattaa ccatcgcggc cgcgactgcg tgccggagtg
      541 gctccaagcg cacggatagg gcaatatgat cccggttgga gcgggggaag cacgcatatg
      601 tccgacgccg atgacgccgc ctgggacaag gcggcgccga cgctttacgc gttcgagttt
      661 cgcggcacgg gaagcgaata tttccgcatc tggatcgtca acctggcgct gacgatcctg
      721 acgctcggcg tgttcagcgc ctgggcgaag gtgcgcactt ggcgctattt ctacggcaac
      781 accttcgtcg gcggccacgc cttcgactat cacgcttcgc cgtggcgcat cctgatcggg
      841 cgggcgatcg ccgtgggcct gttcgtcggc tacaatgtga ccgccgcctt caagccgtta
      901 tacgccctgc cctggattct gatcttgggc ttcgcctttc cctggctggt caattccagc
      961 ctgcggttca acgcgcgcaa caccagctac cgcaacgtgc ggttcaattt caccggccgg
     1021 tatttcgaag cgctggtcgc ctacgtggtt tggccgctgg ccgggtttgc gaccttgttc
     1081 ctgctgatgc cggcggcgcg gcgcgcccgc gatttcttct tcaccaacaa ccatactttc
     1141 ggcggccgcc gcttccgcac cgaattcacg ccctgggaga tctactcgat ctttctgttc
     1201 ggatttttcc tgttcgtcac cctcgccgcc gtcatcgccg cgggcttctg ggcgaacgtc
     1261 tatatcctgg cgaagggcca caccatcttc tccaaccaga tcgtcgtgtc ggccgccttc
     1321 atcgccattt tggtgttcgc ctatctggct ttcctgatgc tggcgcccgc gatcaacacg
     1381 atggtggtga acctgtcgct caacaacacc aggctcgacg gccgccataa ggtgcaatcg
     1441 cgcatctcgc ccctggctgt ggcctggatc gccgtcacca acggctttct ggtcctcttc
     1501 acgctcgggc ttttctatcc ctgggcgcgg gtgcggctcc gccgttacca gacccgcaag
     1561 ctcgccttgc tgttggacgg caacatcgag gattatgcgt ccgaggcttt cgccacccaa
     1621 agcgcggtcg gcgaggaaat cggcagcttc ttcgccttcg atttcgggct ttagacgcat
     1681 gttggaaggc cgctatttct ttccgcattc cgcccgtttc gtggcggcgc gcgcggccgt
     1741 caccggcgca aagacgctgc gcatcgaaag tctcgacgga accgtgctcg ccgaagtgcc
     1801 gatgcgcaaa gtgcgggtgg cggcgcggct gggccggatc gcgcggcgct tcgaactgcc
     1861 ggacggcgcg cgttacgaaa ccgacgacaa tgacggcgcc gacgacctgc tgcgcgaggc
     1921 gcgccgggct ccgggctggg tcgaccggct cgaacgctcc tggcgcagcg tcctgctctc
     1981 ggttctgctc gccgccggag cggcctacgc cttcgtcttg tacggcatcc cggccatcgc
     2041 cgcggaactg gcgctgcaca cgccctccgg catcgcgcgg gtgatggccg atcagaccat
     2101 cgttgccctc gacggcgtct atctcaaacc ctccaaactc gccgccggcg accgcaagaa
     2161 ggcgcaggcg ctgttcgcgc gcgtcgcggc ccatgcgccg cgcggcaagg acggctaccg
     2221 cctgctgctc cgcggcggcg gcgtcatcgg ccccaacgcc
//
LOCUS       DASWPU010000094         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_25119,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000094 DASWPU010000000
VERSION     DASWPU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25119"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VGC27_02085"
     CDS             <1..407
                     /locus_tag="VGC27_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281393.1"
                     /translation="VVSFRQFWMFRTGDGYFPQQEVLDPEFKNFLPRQYEHYLDARQQ
                     RGVLRFEAVNVVHYPLALLSLAWLALVLRSAVRHRQWQRAALPAFVFLALIGNALVCG
                     LFSGPHDRYQSRLIWLPALVLLLTERPKSLFR"
     gene            complement(482..1732)
                     /locus_tag="VGC27_02090"
     CDS             complement(482..1732)
                     /locus_tag="VGC27_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0281394.1"
                     /translation="MTGSGLRLIACAMALIGLLHTPAFADAPADNPDMSFPDDWFAAD
                     ADALAATVLTPEQAASQSDAVFDLVDRHIFDTASTADARAKARKAALAAVQNGPQTQE
                     TVGKAIDDALQSIGYSHMFLFPPEIAREIVDMTKGKDDGKPAPSAISARMIGDVGVLR
                     VDSFMVPLFTQTALDEGFAKLAKAKVLLIDVSGNGGGNSSSVVALAHPLVGAGKPIAK
                     SVFRLGHAPTPFEQYGPLPDADNHGGAADNALGQVHGVVTWLTGKNAVAPAKRPIFVL
                     IDGHCGSSCEIFSGAIKHYGAATLLGRNTAGKVLGGIAFRPPMKGYMLLLPTSTVYGP
                     GGEVYEGPGVAPDVELQVCQTFAGKTSEEGRKSCLDAALAYITAHPRSAAAGGPAPRS
                     PRQDTKNRQTESTERFGQASPSGG"
     gene            1985..2644
                     /gene="rpe"
                     /locus_tag="VGC27_02095"
     CDS             1985..2644
                     /gene="rpe"
                     /locus_tag="VGC27_02095"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY0281395.1"
                     /translation="MSQVRIAPSILSADFARLGAEIAALEAAGADFIHVDVMDGHFVP
                     NLTIGPPVVKALRPHARRVPFDVHLMISPVDPYLEDFAAAGADGITVHPEAGPHIYRS
                     LQHIRDLGKRPGIVLNPGTPIDHIDPVLDLVDLVLIMTVNPGFGGQSFLASQLRKIEA
                     AAERIARTGRDIALEVDGGITPKTAKQVISAGANVLVAGTAVFEGGAGSYAANIAALR
                     G"
BASE COUNT          434 a          888 c          947 g          525 t
ORIGIN      
        1 tggtcgtgtc gttccgtcaa ttctggatgt tccgcaccgg cgacgggtat tttccccagc
       61 aggaggtcct cgacccggaa ttcaagaatt tcctgccgcg ccagtacgag cattatctcg
      121 acgcgcgcca acagcgcggc gtcttgcgct tcgaggccgt gaacgtcgtc cattacccgc
      181 tggccttgct gtcgctggcc tggctggcct tggtcctgcg cagcgccgtc cggcaccggc
      241 aatggcagcg tgccgcgctg ccggcctttg tattcctggc gctgatcggc aatgcgctgg
      301 tctgcggcct gttctccggg ccgcacgacc gctaccagtc gcggctgatc tggctgccgg
      361 cgctggtttt gctgttgacg gagcggccca agagcctgtt tcggtaatcc tcgcggccgt
      421 gacgattacc gaaacaggct ctaacctcga actcaccttg cagcggaggc cggaatccgg
      481 cttatccgcc cgatggtgaa gcctgcccga atcgctccgt cgattctgtc tggcgatttt
      541 tcgtgtcttg gcgaggagat cgcggcgccg gcccgcctgc cgccgctgat cgggggtgcg
      601 ccgtgatata ggccagcgcg gcatcgaggc aggacttgcg gccttcctca ctcgtcttgc
      661 cggcgaaggt ctggcagact tgcagttcga cgtcaggcgc cacgccaggg ccttcgtaga
      721 cctcgccgcc ggggccgtag accgtgctgg tcgggagcag cagcatgtag cccttcatcg
      781 gcgggcggaa ggcgatgccg ccgagcacct tgccggcggt gttgcggccg agcagcgtag
      841 cggcgccgta atgcttgatg gcgccggaga agatttcgca ggacgagccg caatgaccgt
      901 cgatcagcac gaagatcggg cgtttcgccg gtgcgaccgc gttcttgccg gtcagccagg
      961 tgacgacgcc gtgcacttgc cccagagcat tgtcggcggc gccgccgtga ttgtcggcat
     1021 cgggcagcgg gccatattgc tcgaatggcg tcggcgcatg gccgagtcgg aacacactct
     1081 tggcgatcgg tttgccggcg ccgaccagcg ggtgcgccaa ggcgaccacc gacgaggaat
     1141 tgccgccgcc gttgccgctg acgtcgatca gcaggacctt cgccttggcc agcttggcga
     1201 agccttcgtc cagcgccgtt tgcgtgaaca gcggcaccat gaagctgtcg acccggagga
     1261 cgccgacatc gccgatcatc cgggccgaaa tcgccgaggg ggccggcttg ccgtcgtcct
     1321 tgcccttggt catgtcgacg atctcgcggg cgatctccgg cgggaacagg aacatgtggg
     1381 aatagccgat gctctgcagc gcgtcgtcga tggccttgcc gaccgtctcc tgggtctgtg
     1441 ggccgttctg caccgccgcc agcgccgcct tgcgggcctt ggcgcgcgcg tcggcggtcg
     1501 aggcggtatc gaagatatgg cggtccacga gatcaaacac cgcatcgctc tggcttgcgg
     1561 cttgctccgg cgtcagaacg gtagcggcca gagcgtcggc atcggcggcg aaccaatcgt
     1621 cggggaagct catgtccggg ttatcggccg gcgcgtcggc aaaggcgggc gtatggagaa
     1681 gaccgatgag cgccatggcg caggcaatca ggcgaagacc ggaaccggtc atgacggctt
     1741 gcatcccttg tgatttccag cggcgcgcat agtgggcgac aagccgatcg cgtgtccaga
     1801 gagcgccgcc tcacatttgc gcgcggacaa aatcgcgccg gccggccggg catggcgcaa
     1861 aaaagctgtt tcgttcagcc gcgaatccgg caaaaacaga gaatgcaggc gcattgcgcc
     1921 gttggcggat gattctttgt ccggcgacag ttctcttttg gggatagtgg gttagtatcg
     1981 caatatgtct caggttcgca ttgctccttc cattctgtcc gccgatttcg cgcgcctcgg
     2041 tgcggagatc gcggcgctgg aggcggccgg cgccgatttc atccatgtcg atgtgatgga
     2101 cggccatttc gtgcccaacc tcaccatcgg cccgccggtg gtcaaggcgc tgcgcccgca
     2161 cgccaggcgc gtgccgttcg acgtgcatct gatgatctcg ccggtcgatc cctatcttga
     2221 ggacttcgcc gcggcggggg cggacggcat caccgttcat cccgaggccg ggccgcatat
     2281 ctaccgctcg ctgcagcata tccgcgatct cggcaagcgg ccgggcatcg tgctcaatcc
     2341 ggggacgccg atcgaccata tcgatccggt gctcgatctg gtcgatctgg tcctgatcat
     2401 gaccgtcaat ccgggcttcg gcggacagag ctttctcgcc agccagctca ggaagatcga
     2461 ggcggccgcc gaacgcatcg ccaggacggg ccgcgatatc gcactcgaag tcgatggcgg
     2521 tataacgccc aagacggcga agcaggtgat ttccgccggg gccaatgttc tggtcgccgg
     2581 caccgcggtg ttcgagggcg gagccgggag ctacgccgcc aacatcgccg cgttgcgagg
     2641 gtaaaagtgt gtccgcaggc cctgcacagc gcacgaaggc gcccctggcg cttcttctcg
     2701 aagcggccgg ggtggcgtgg tggcggctgc gcggcggctg gcggcgctgg gtgcggcgga
     2761 cctggttcta tcgccggctg ctgaaagggc cgct
//
LOCUS       DASWPU010000095         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_68121,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000095 DASWPU010000000
VERSION     DASWPU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68121"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1589
                     /gene="sufB"
                     /locus_tag="VGC27_02100"
     CDS             138..1589
                     /gene="sufB"
                     /locus_tag="VGC27_02100"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY0281396.1"
                     /translation="MAAVKETAQAVADLGDRYKYGFVTDIEMERAPKGLTEDTVRYIS
                     AKKGEPEWMLNWRLAAYRRWREMTEPNWARIHYPKIDYDDAYYYAAPKSAEGPKSLED
                     VDPKLLETYKKLGIPLSEQKQLAGVAVDAVFDSVSVATTFKEKLNEAGVIFCPISEAI
                     RDYSDIVRKYLGSVVPVSDNFFATLNSAVFSDGTFVYVPKGVRCPMELSTYFRINASQ
                     TGQFERTLIIADEGSYVSYLEGCTAPMRDENQLHAAVVELVALEDAEIKYSTVQNWYP
                     GDENGLGGIYNFVTKRGDCRGARSKISWTQVETGSAITWKYPSCILRGDDTVGEFYSI
                     AIVNHYQQADTGTKMIHLGKNTRSHIVSKGISAGKAQNTYRGLVSVHPKAAKARNHTQ
                     CDSLLIGSKCGAHTVPYVESRNPSARVEHEATTSKIAEDQLFYCLQRGISPEEAVALI
                     VNGFCKEVLQQLPMEFAVEAQKLVGISLEGSVG"
BASE COUNT          353 a          504 c          524 g          303 t
ORIGIN      
        1 cgtgccggaa ccgctcgcac gttgcggtct gcgggtcagc ttcggctgga acagccgcga
       61 ggaagacgtg gacgctgtta tcgcatcgct ctccaacctg ctcgcgcgcg tcgcgcgggc
      121 cgccgcgtga gggcgccatg gctgccgtaa aggaaaccgc acaagccgtc gccgatctgg
      181 gcgaccgcta caaatacggc ttcgtcaccg acatcgagat ggagcgcgcg cccaaaggct
      241 tgactgagga cacggtgcgg tacatttcgg ccaagaaggg cgaaccggag tggatgctga
      301 actggcggct tgccgcctat cggcgctggc gcgagatgac cgagcccaat tgggcgcgca
      361 tccattatcc caagatcgac tacgacgacg cctattacta cgccgcgccc aagagcgcag
      421 agggcccgaa gagcctggaa gacgtcgatc ccaagctgct tgagacctac aagaagctcg
      481 gcattccgct ttcggagcag aagcagctcg cgggcgtggc ggtggacgcg gtgttcgatt
      541 ccgtgtccgt cgcgaccacc ttcaaggaga agctgaacga ggcgggcgtg atcttctgcc
      601 cgatctccga agccatccgc gactattcgg acatcgtgcg caaatatctg ggcagcgtcg
      661 tgccggtcag cgacaatttc ttcgccacgc tgaattcggc ggtgttttcc gacggcacct
      721 tcgtttacgt gccgaagggc gtgcgttgcc cgatggagct ttcgacctat ttccgcatca
      781 acgccagcca gaccggccag tttgaacgca cgttgattat cgccgacgaa ggttcctacg
      841 tgtcctatct cgaaggctgc accgcgccga tgcgcgacga gaaccagttg catgccgcgg
      901 tggtggaact ggtggcgctg gaggacgcgg aaatcaaata ttccacggtg cagaactggt
      961 atccgggcga cgaaaacggg ctcggcggca tctacaattt cgtcaccaag cgcggcgact
     1021 gccgcggcgc gcgctcgaag atctcctgga cgcaggtgga aaccggttcg gcgatcactt
     1081 ggaaatatcc gagctgcatc ctgcgcggcg acgatacggt cggcgagttc tattccatcg
     1141 ccatcgtgaa ccattaccag caggccgaca ccggcaccaa gatgatccat ctagggaaga
     1201 acacgcgctc gcacatcgtc tccaagggca tcagcgccgg caaggcgcag aacacctatc
     1261 gcggcttggt cagcgtgcat cccaaggctg cgaaggcgcg caaccatacg caatgcgact
     1321 ccctgctgat cggcagtaaa tgcggcgcac acactgtgcc ctatgtcgaa agccgcaatc
     1381 cgtcggcccg cgtcgagcac gaagcgacga catcgaagat cgccgaagat cagttgttct
     1441 attgcttgca gcgcgggatt tcgcccgagg aggcggttgc gctgatcgtc aacggcttct
     1501 gcaaggaagt attgcagcaa ttgccgatgg aattcgccgt ggaagcgcaa aaactagtcg
     1561 gtataagttt ggagggatcg gtcggatgac acaccgtttc cgattattgt catggcccgc
     1621 aacagcgggc catccaggtg aaaacgggct cgatcagcgt gcggacgcgc gctggctgga
     1681 tggc
//
LOCUS       DASWPU010000096         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_19096,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000096 DASWPU010000000
VERSION     DASWPU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19096"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VGC27_02105"
     CDS             <1..811
                     /locus_tag="VGC27_02105"
                     /inference="COORDINATES: protein motif:HMM:NF019180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HEY0281397.1"
                     /translation="GRTREARSGRGNTDITPHRKIRAADFSLARCRCSPPSRGETKSF
                     SILLDGKPIKTPLRATLALPTRALAEAVAEEWRGQGELVVPTTMWLTKLANTAIDRVM
                     GQEEHVIAQVLAYANDLLCYRAQAPAGLVARQNEDWDPLLEWAAERHGARLLVRAGIS
                     HIAQPEEAVAALRRALAEHDPFVLTALHAAATICGSLVLALALADGRLDAEEAFALSR
                     LDERYQAERWGVDEEAEMRARVLAAELAAAARFMLLSKGEPDYAHPSTSSG"
     gene            889..>1813
                     /locus_tag="VGC27_02110"
     CDS             889..>1813
                     /locus_tag="VGC27_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY0281398.1"
                     /translation="MKHVIHELEERRSRARAGGGEAKIKQQHARGKLTARERIDLLLD
                     PGSFEEFDMFVEHRNADSHIDKSRIPGDGVVTGWGTINGRMAYVFAKDFTVFGGSLSE
                     AHAQKICKIQDMAMQNGAPVIGLYDAGGARIQEGVAALAGFGEIFKRNVLASGVVPQI
                     SVIMGPCAGGDVYSPALTDFIFMVRGTSYMFITGPDVTKTVDEGDFFEISESFARNIV
                     TGFGRIAGHTVGFVANQPMVLAGVLDSDASRKAARFVRFCDAFNIPIVTFVDVPGFLP
                     GTDQEYGGLIKHGAKLLFAYSQCTVPLVTVIT"
BASE COUNT          314 a          591 c          600 g          308 t
ORIGIN      
        1 gggtcgaaca cgcgaagcgc gttcggggag gggtaatacc gacattaccc cccaccgaaa
       61 aatccgcgcg gcggattttt cgctcgcgcg ctgccgctgc tcacccccct caagggggga
      121 gacaaaatca ttttctatcc tcctcgacgg caaaccgatc aagacgccgc tgcgcgccac
      181 gctggcgctg cccacgcgcg cgctggccga ggcggtcgcg gaagaatggc gcgggcaggg
      241 ggaattggtc gtgccgacga ccatgtggct caccaagctc gccaacacgg ccatcgaccg
      301 tgtcatgggc caggaagagc acgtcatcgc gcaggtcctc gcttatgcga acgatctact
      361 ctgttatcgc gcgcaggctc cggccgggct ggtcgcgcgc cagaacgaag attgggaccc
      421 gctgctcgaa tgggcggccg agcgccatgg cgcaaggctg cttgtccgag ccgggatcag
      481 ccacatcgcc cagccggagg aggccgtggc ggcgctgcgc cgcgcactgg cggagcatga
      541 tccgttcgtc ctaacggcgc tgcacgccgc cgcgaccatc tgcggctcgc tggttctggc
      601 gctggctttg gccgacggcc ggctcgacgc cgaagaggca ttcgcgctct cccgtctgga
      661 cgagcgctat caggcggaga gatggggcgt ggacgaggaa gccgaaatgc gggcgcgcgt
      721 cctggctgcc gaactggcgg ccgcggccag attcatgctc ttgtccaagg gcgaaccgga
      781 ttacgctcac ccttcgacaa gctcagggtg agggattagt ttgaagctca gaatgagggg
      841 cttgcttggc cttcatgctg agcttgtcga agcatgcggg atactgccat gaagcacgtc
      901 attcacgaac tggaagagcg ccgcagccgc gcgcgggcgg gcggcggcga ggcgaagatc
      961 aagcagcagc acgcgcgcgg caagctgacg gcgcgcgaac gcatcgacct gttgctcgat
     1021 cccggttcgt tcgaagagtt cgacatgttc gtcgagcatc gcaacgccga ctcccacatc
     1081 gacaagtccc gtattccggg cgacggcgtg gtgacgggct ggggtacgat caacgggcgc
     1141 atggcctatg tcttcgccaa ggatttcacc gtgttcggcg gctcgctgtc cgaggcgcac
     1201 gcccagaaga tctgcaagat ccaggacatg gcgatgcaga acggcgcgcc ggtgatcggg
     1261 ctttacgacg cgggcggcgc gcgcatccag gaaggcgtcg ccgcgctggc cggcttcggc
     1321 gaaatcttca agcgcaacgt gctggcttcg ggcgtggtgc cgcagatatc cgtcatcatg
     1381 gggccgtgcg cgggcggcga cgtttattcc cccgcgctga ccgatttcat cttcatggtg
     1441 cggggcacct cctacatgtt catcaccggg ccggacgtga ccaagacggt ggacgagggc
     1501 gatttcttcg agatctcgga atcgttcgcc cgcaacatcg tcaccggctt cggccgcatc
     1561 gccggccaca cggtcggctt cgtcgccaac cagccgatgg tgctcgccgg cgtgctcgac
     1621 tccgatgcga gccgcaaggc tgcacggttc gtgcgcttct gcgacgcctt caacatcccg
     1681 atcgtcacct tcgtcgacgt gcccggcttc ctgcccggca ccgaccagga atacggcggc
     1741 ctgatcaagc acggggccaa gctcctgttc gcctacagcc agtgcacggt gccgctcgtc
     1801 accgtgatca ccc
//
LOCUS       DASWPU010000097         6085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_68133,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000097 DASWPU010000000
VERSION     DASWPU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68133"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VGC27_02115"
     CDS             <1..316
                     /locus_tag="VGC27_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281399.1"
                     /translation="VTQVRVHAPDDTPSLLDPSAYQVDVASAPARLLRKNAARIDLRA
                     VDAVEIAFTAGYGAADAVPQPIKQAILHIVADLYSHRGDEAEIVSSAAAALLAPYRVF
                     KL"
     gene            313..627
                     /locus_tag="VGC27_02120"
     CDS             313..627
                     /locus_tag="VGC27_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEY0281400.1"
                     /translation="MIGELDQRAALQAKVLTPDGGGGFSESWQTFATAWVKVAPVGAN
                     DTFGPDALEARVRHRLALRRRGDLAAGQRVLVGARSFKVHALLDEGPRAEIVTLLCEE
                     LP"
     gene            624..1031
                     /locus_tag="VGC27_02125"
     CDS             624..1031
                     /locus_tag="VGC27_02125"
                     /inference="COORDINATES: protein motif:HMM:NF022810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEY0281401.1"
                     /translation="MSSSWALQQAVFAALAMSDALKEIAGDPPRVFDAVPRAGAFPYI
                     VVGEDSETNWDTATESGSEHALAIHIWSRGGGRKESKLAAEAVREALHGASLALDGHT
                     LVDLRYLSADFARESDGETFRATLRFRAVTEPN"
     gene            1200..1616
                     /locus_tag="VGC27_02130"
     CDS             1200..1616
                     /locus_tag="VGC27_02130"
                     /inference="COORDINATES: protein motif:HMM:NF017961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEY0281402.1"
                     /translation="MSAQRGKDLLIKIGDGTNPEAFITVAGLRATTLAFNAQNVDVTN
                     SDSADMWRELLAGAGVKSATISGSGVFKDAASDAALRAAFFNQTLCNWQIVIPHFGIV
                     AGPFKLASLQYEGPYDGELKLSLSLASAGALTFTAT"
     gene            1792..2154
                     /locus_tag="VGC27_02135"
     CDS             1792..2154
                     /locus_tag="VGC27_02135"
                     /inference="COORDINATES: protein motif:HMM:NF023264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTA-gp10 family protein"
                     /protein_id="HEY0281403.1"
                     /translation="MPNRIRGEAELIAGGKSFRLLLTLGALAEIEDGLGLDDLAGIAA
                     RLKQARASDLAVVAAALLRGGGHEITPADVLRLNCDLGALVAAVAQAFDRAGLPRAGE
                     EGGEASGPFPGTPGLRSD"
     gene            2160..2294
                     /locus_tag="VGC27_02140"
     CDS             2160..2294
                     /locus_tag="VGC27_02140"
                     /inference="COORDINATES: protein motif:HMM:NF021092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HEY0281404.1"
                     /translation="MGLKPKTFWNMSLAEWRAMLAGKRPRRSSLGRRELDDLMQHYPD
                     "
     gene            2298..2831
                     /locus_tag="VGC27_02145"
     CDS             2298..2831
                     /locus_tag="VGC27_02145"
                     /inference="COORDINATES: protein motif:HMM:NF021251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HEY0281405.1"
                     /translation="MPDPTADVEASLARAAKAFADFANGPVASASKTIDEAVTRSFRS
                     VENTIARAALSGKTSIGKLVDSILADFDRVATRQFIIKPIEGVIGSLISSLLPVAGAR
                     AAGGPVEAGSAYLVGERGPELFVPSANGAIAPNGAPARPAITLNVQARDAQSFLKSES
                     QIAAMLSRALARGQRNM"
     gene            2968..3594
                     /locus_tag="VGC27_02150"
     CDS             2968..3594
                     /locus_tag="VGC27_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HEY0281406.1"
                     /translation="MTFHEIRFPTAIAFHSTGGPERKTEIVALGSGFEERNGVWAGSR
                     RRYDVGSGVKTLDDLHAVVAFFEARMGRLYAFRFKDFSDFKSCAPGASVAPTDQSVAT
                     GDGATKIFQLAKTYTSGPSGWTRAVRKPVAGSVRMALNGVEQTGGWSVDTATGLVTFA
                     SAPANGVSIAAGFEFDVPVRFDTDSLLVNLANFQAGEIPSIPIVEVLL"
     gene            3808..4695
                     /locus_tag="VGC27_02155"
     CDS             3808..4695
                     /locus_tag="VGC27_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HEY0281407.1"
                     /translation="MKTLPPGLQAHLDSGTTTLCWCWKIVHRDGVALGFTDHDAPVSF
                     GGLVYEAAAGFTASEVQSTLGLAVDNLTVMGALSSAALNEKDLAAGLYDNAAVEIWRV
                     NWSTPAERVLMRKGSLGEVKRGKTGFAAEVRGLAHILNQPVGRAYGHGCDADLGDGRC
                     KLALADYTATGTIAGVLDARRFTVAGLGAFANDWFTGGKLAFTGGGNSGRAMEVKRHA
                     VAGAAVTVELWQAMSEPVMAGDAFAVSAGCDKQFSTCKSRFANAVNFRGFPTMPGNDT
                     VLSHPASSTLLDGGSRYGK"
     gene            4814..5221
                     /locus_tag="VGC27_02160"
     CDS             4814..5221
                     /locus_tag="VGC27_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY0281408.1"
                     /translation="MRIARAWIGTPYVHQASVKGAGCDCLGLLRGVWRELNGVEAEAP
                     PPYSPDWAEAKAKETLREALARHLSEIDPRHAAPGDIVLFRMVARGPAKHCAIVGEKN
                     GTLTLIHARQNKRVSEEAFSPAWRRKLAYAFRI"
     gene            5317..6054
                     /locus_tag="VGC27_02165"
     CDS             5317..6054
                     /locus_tag="VGC27_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0281409.1"
                     /translation="MNANIRLILMGLVMVAAVSGTVYLYSMSVPFERHRQQCRNGADK
                     AAIEACTAVIESEIAGSNDAAEAYGWRANRYGGLKQYDRAIADFDAALRLNPDDADAL
                     AGRGWTYSALGQHGRALRDFDRMVNKRANAVTYNMRCWARATWGEQLDAALADCNAAL
                     KLKPDYDAALDSRAFVNFRMGNYSRAVGDASGALSLNPELAPSLYVRGLARLGEGNVK
                     NGEADIAAAKKLDPEIEETYAGYGVKL"
BASE COUNT         1087 a         1982 c         1986 g         1030 t
ORIGIN      
        1 cgtgacgcag gtgagggtcc acgcgcccga cgatacgccg agtctgctcg atccctcggc
       61 ctatcaggtg gacgtggcct ccgcgcccgc gcgcctgttg cgcaagaacg cggcgcgcat
      121 cgatctgcgc gccgtcgatg ccgtcgagat cgcgttcacc gccggttacg gcgccgccga
      181 tgcggtgccg cagccgatca agcaggcgat cctgcacatc gtcgccgatc tttacagcca
      241 tcgcggcgac gaagcggaga tcgtctcatc cgccgccgca gctctgctgg cgccttaccg
      301 ggttttcaaa ctgtgatcgg cgaactcgac cagcgcgccg cgcttcaggc gaaagtgctg
      361 acgcccgacg gcggcggcgg tttcagcgaa agctggcaga ccttcgcgac ggcgtgggtg
      421 aaggtcgcgc ccgtcggtgc gaacgacacg ttcggccccg acgcgctgga agcgcgcgtg
      481 cgccaccgtc tcgcgctgcg ccggcgcggc gaccttgccg ccggtcagcg cgtgctggtc
      541 ggcgcgcgca gcttcaaggt ccacgcgctg ctcgatgaag gaccgcgggc cgaaatcgtt
      601 actttgttgt gcgaggaatt gccatgagct cgagctgggc gctgcaacaa gccgtgttcg
      661 ccgccttggc gatgagcgac gccctcaagg agatcgcggg cgatccgccg cgcgtcttcg
      721 acgccgtgcc gcgcgccggc gcctttccct atatcgtcgt aggcgaggat agcgaaacca
      781 actgggacac cgcgacggag tccggcagcg agcatgcgct ggcgatccat atctggtcgc
      841 gcggcggcgg gcgcaaggag agcaagctgg cggccgaggc cgtgcgcgag gcgcttcatg
      901 gcgcctcgct ggcgctcgac ggccacaccc tggtcgatct gcgttacctc tcggccgact
      961 tcgcgcgcga gagcgacggc gaaaccttcc gcgccacgct gcgcttccgc gcggtgaccg
     1021 aaccaaattg aagaaacaca agatcacaat tgtcatggcc cgcgaatgcg ggccacccag
     1081 ttggggacaa tctttctgct gaagaattga actgagtgaa ctggtcgata ccgagcgcat
     1141 gtcacctgga tggcccgcat caggcgggcc atgacaacgt aatttaggga gtgcaaccca
     1201 tgtccgccca acgcggcaaa gatctgctga tcaagatcgg cgacgggacc aaccccgaag
     1261 ccttcatcac cgtcgccggg ctgcgcgcca ccacgcttgc cttcaacgcg cagaatgtgg
     1321 acgtcaccaa ttcggattcc gccgacatgt ggcgcgaact gctggcgggc gcgggcgtga
     1381 agtccgcgac catctccggc tccggcgtat tcaaggacgc cgcctccgac gccgctttgc
     1441 gcgccgcctt cttcaatcag acgctgtgca actggcagat cgtgatcccg cacttcggca
     1501 tcgtggccgg cccgttcaag ctcgcctcgc tgcaatatga aggcccgtat gacggcgaac
     1561 tgaaactttc gctgtcgctg gcgagcgcgg gcgcgctgac gttcacggca acataagaag
     1621 cgaatagcga atagagagta gcgaagtggg ttttctgccc ccgccctgcc caccgaagct
     1681 ttagcgtagg tggggagcgg gggaagtgtc acccgaagct ccaaaggagc gaagggtgac
     1741 gaagggggcc ccgttcgcca atccccattt gcctgcaaag gaaacggaca catgcccaac
     1801 cgcatccgcg gcgaagccga gctgatcgcc ggcgggaaat ctttccgcct cttgctcacg
     1861 ctcggcgcac tggccgaaat cgaggacggg ctggggctcg acgatctggc cgggatcgcc
     1921 gcgcgcttga agcaggcgcg cgcttcggat ctcgccgtgg tcgcggcggc gctgctgcgc
     1981 ggcggcggcc atgagatcac gcccgccgat gtgctgcgtc tcaattgcga tctgggcgcg
     2041 ctggtggcgg cggtggcgca ggctttcgac cgcgccggtc tgccgcgtgc gggcgaagag
     2101 ggcggagaag cgtccggccc ttttcctggg acgcctggct tgcgctcgga ttaggcgcct
     2161 tggggctgaa gccgaaaacc ttctggaaca tgtcgctggc cgaatggcgc gccatgctgg
     2221 cgggcaagcg cccgcgccgc tcgtctctcg gccgccgcga actcgacgat ttgatgcaac
     2281 actacccgga ctgaaccatg cccgatccaa ccgcagacgt ggaagcgagc ctggcgcgcg
     2341 ccgccaaggc gttcgccgat ttcgccaatg gccccgtcgc cagcgcgagc aagaccatcg
     2401 acgaggcggt gacgcgctct ttccgctcgg tggaaaacac catcgcgcgc gcggcgctgt
     2461 cgggcaagac ctcgatcggc aagctggtcg attccatcct ggccgatttc gatcgcgtcg
     2521 ccacccgcca attcatcatc aagccgattg aaggcgtgat cggttcgctg atctcctcgc
     2581 tgctgccggt tgccggcgcg cgcgccgccg gcggaccggt cgaggcgggc agcgcttatc
     2641 tcgtcggcga gcggggaccg gaattgttcg tgccgtcggc gaacggcgcg atcgcgccga
     2701 acggggcgcc cgcgcggccc gcgatcacgc tcaacgtgca ggcgcgcgac gcgcagagct
     2761 tcctcaaatc cgaaagccag atcgcggcga tgctgtcgcg cgcgctggcg cgcggtcaaa
     2821 ggaatatgtg aatagaaccc aacttgtcat ccccggcgag ccgcgccgtt cgcgcggcga
     2881 gggaagggga tccagaagcc tgcgcagacc catggattcc cttcccctcg cttcactcgg
     2941 ccaggaatga caagcgagat gtatcccatg accttccacg aaatccgctt ccccaccgcc
     3001 atcgcctttc attcgacggg cgggccggag cgcaagaccg aaatcgtcgc gctcggttcc
     3061 ggcttcgagg agcgcaacgg agtgtgggcg ggttcgcgcc gccgctacga tgtcggctcc
     3121 ggcgtcaaga cgctcgacga tctgcacgcc gtcgtcgctt tcttcgaggc gcgcatgggc
     3181 cggctctatg ctttccgctt caaggacttt tccgatttca aatcctgcgc gcccggcgcc
     3241 agcgtggcgc ccacggatca atccgtcgcc acgggcgacg gcgcgacgaa aatcttccag
     3301 cttgcgaaaa cctacacctc cggtccttcg ggctggacgc gcgccgtgag aaagccggta
     3361 gcgggtagcg tgcgcatggc gctgaacggc gtcgaacaga caggcggctg gagcgtggac
     3421 accgccaccg gcctcgtcac cttcgcctcc gcgcccgcga acggcgtttc catcgccgcc
     3481 ggattcgaat tcgacgtgcc ggtgcgcttc gacacggatt cgcttctggt caatctggcg
     3541 aatttccagg cgggagaaat tccaagcatt ccgattgtcg aggtgctgct gtgaccgaag
     3601 aacgcaaatc aaaaatgtca tggcccgcgc ccttcggcac gccaagccgc ctcctcctga
     3661 gcttgtcgaa ggagtgggcg gcttggctgc tcaggatgcg ggccacccag gtgacatttt
     3721 ccagctcttt caggtttcaa ccgggtcacc cgtccgtacg ctgtcgctac gaactcgcgg
     3781 gtgatgacag caataaagaa tgagcaaatg aagactttac cccccggcct gcaagcccat
     3841 ctcgattccg gcaccaccac gctgtgctgg tgctggaaga tcgtccaccg cgacggcgtg
     3901 gccctcggct tcaccgacca cgatgcgccc gtctcgttcg gcggcctcgt ctacgaagcc
     3961 gccgccggct tcaccgccag cgaagtgcaa tcgacgctcg gcctggcggt ggacaatctg
     4021 acggtgatgg gcgcgctgtc gtcggcggcg ctgaacgaga aagatctcgc cgccgggctt
     4081 tacgacaatg ccgcggtgga aatctggcgg gtgaactggt ccacgcccgc cgagcgcgtg
     4141 ctgatgcgca agggtagcct cggcgaggtg aagcgcggca agacgggttt tgcagccgaa
     4201 gtgcgcggat tggcgcatat cctgaaccag ccggtcggcc gcgcctacgg ccatggctgc
     4261 gacgccgacc tgggcgacgg gcgctgcaag ctcgcactcg cggattacac cgcaacggga
     4321 accatcgccg gcgtgctcga cgcgcggcgt ttcaccgtgg cgggcctcgg cgcctttgcg
     4381 aacgactggt tcacgggcgg caagctcgct ttcaccggcg gcggcaattc cggccgcgcg
     4441 atggaagtca aacgccatgc cgtagcgggc gccgccgtca ccgtcgaact gtggcaggcg
     4501 atgagcgaac ccgttatggc gggcgacgcc ttcgccgtca gcgcgggctg cgacaagcag
     4561 ttttccacct gcaaatcgcg cttcgccaac gcggtgaatt tccgcggctt tcccaccatg
     4621 ccgggcaacg acacggtgct gtcccatccc gcctcttcga cgctgctcga cggaggcagc
     4681 cgctatggca aatagaaaca caaaacaaag aatgtcatgg ccagcgaatg cgggccaccc
     4741 aggtgacatg tgcgcgatat gtcaggcttc aactgggtcg cccgcagttg cgggcgatga
     4801 cacgatagag attgtgcgca tcgcccgcgc ctggatcggc acgccctatg tgcatcaggc
     4861 cagcgtcaag ggcgcgggct gcgattgcct cggcctgttg cgtggcgtct ggcgcgaact
     4921 caatggcgtg gaggcggaag cgccgccgcc ttattcgccc gattgggccg aagcgaaggc
     4981 caaggaaacc ctgcgcgagg cgctggcgcg gcatctgagc gaaatcgatc cgcgccatgc
     5041 tgcgcccggc gacattgttc tgttccgcat ggtggcgcgc ggcccggcca aacattgcgc
     5101 catcgtgggc gaaaagaacg gcacgctgac gctgatccat gcgcgccaaa acaagcgcgt
     5161 gagcgaggaa gcattctctc ccgcctggcg gcgcaagctc gcttacgcct ttcggatttg
     5221 aacactgaaa agcgttgcaa cccgcatggc gacaggggag aaaccccgct atgatggaac
     5281 agcgccgtca ttgcgggcgg ccgggcgggg caagagatga acgcgaatat ccggctcatt
     5341 ttgatgggcc tggtgatggt ggccgcggtt tccggcacgg tctatttgta ttccatgagc
     5401 gtgccgttcg agcgccaccg gcagcaatgc cgcaacgggg cggacaaggc cgcaatcgaa
     5461 gcctgcaccg cggtcatcga atcggaaatt gccggcagta acgatgcggc cgaagcatat
     5521 ggatggcgcg ccaaccgtta tggcggtttg aagcaatatg atcgcgcgat agcggatttc
     5581 gacgcggcgc tgcgcctgaa cccggacgac gccgatgcgc ttgcgggccg cggctggacc
     5641 tacagcgcgc tcggtcaaca tgggcgcgca ttacgggatt tcgaccggat ggtgaacaag
     5701 agagccaatg cggtcaccta caacatgcgc tgctgggcgc gcgccacctg gggcgaacag
     5761 ctcgacgcgg cgctggccga ctgcaacgcg gccttgaagc tcaagccgga ttatgacgcc
     5821 gctctcgatt cgcgcgcctt tgtgaatttt cgcatgggca attacagccg cgcggtcggc
     5881 gacgcctccg gggcgctcag tctcaacccg gaactcgctc cctcgctgta tgtccgcggc
     5941 ctcgcccgct tgggcgaggg taatgtgaag aacggcgaag ccgacatcgc ggcggcgaag
     6001 aagctcgatc ctgagatcga agaaacctat gccggctacg gcgtgaagct ttagcgtttc
     6061 tattgccttc ccctcaaggg taggt
//
LOCUS       DASWPU010000098         3478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_25134,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000098 DASWPU010000000
VERSION     DASWPU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25134"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VGC27_02170"
     CDS             <1..1290
                     /locus_tag="VGC27_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY0281410.1"
                     /translation="TSAATSPTWKSGCARPPPTLFEYLPEDALVFCDESHVTVPQIGA
                     MYKGDYRRKFTLAEYGFRLPSCIDNRPLKFEEWDAMRPQTFYVSATPGHWELGATRGV
                     FAEQVIRPTGLIDPPVEIRPVESQVDDLIAECRLVAKKGYRALVTTLTKKMAEDLTEY
                     MHEQGIRVRYMHSDVETLERIEIIRDLRLGAFDVLVGINLLREGLDIPECALVAILDA
                     DKEGFLRSETSLIQTIGRAARNVDSNVILYADHVTGSMQRAMAETTRRREKQHAYNQA
                     HGITPASIKKQIGDIMGSMYERDHVTVGAGELGFAEDGKEFIGHNIRGHIADLEKRMR
                     EAAADLEFEEAARLRDEIKRLTAVELAVADDPFARQSEVERAVDDAFLNAVRDGSSPP
                     PGGEVGPAKRGRMGGKRTKLKRAGRPNVPKTFGSRSK"
     gene            1558..2352
                     /locus_tag="VGC27_02175"
     CDS             1558..2352
                     /locus_tag="VGC27_02175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY0281411.1"
                     /translation="MQLIVTSPPYNIGKSYEARSSLDSYIQAQTQVISECVRLLHPRG
                     SLCWQVGNHVFKGEIIPLDIVLYPIFREHGLKLRNRIVWHFEHGLHCSKRLSGRYETI
                     LWFTKGDDYIFNLDAIRVPSKYPGKKYFKGPKAGQLSCNPLGKNPGDVWVIPNVKNNH
                     VEKTIHPCQFPVELIERLVLGLTEPRGAVFDPYMGVGTAVVAAVKHGRVGYGCDVVQD
                     YVDIAWERVHALKSGTLKTRPMNKPIYDPNKPYGGHKIVPLFRKMG"
     gene            2349..3071
                     /locus_tag="VGC27_02180"
     CDS             2349..3071
                     /locus_tag="VGC27_02180"
                     /inference="COORDINATES: protein motif:HMM:NF020757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HEY0281412.1"
                     /translation="MKLRIFSYRYAQEILQHPNNVVAWNEIEKILSRAPIFIYPGKSK
                     NQKLQIVQQLMNTYFDRRFAVDAVWKYHPLATKIAKSSLAADFRKEFGSITVQAEIQF
                     GNMARWYSDIFKFQTAYSQSLIQCGLCVVPTGEMAKAIDSNVAHYERTIRELPSANLS
                     ITLPILLVGLEQDKRTPVVDVRACQFASLKEIVGKGNSENRWRIINGYVSGADMASIG
                     PESDTGPMLLDEIDEADTEDSD"
     gene            3212..>3478
                     /locus_tag="VGC27_02185"
     CDS             3212..>3478
                     /locus_tag="VGC27_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0281413.1"
                     /translation="MRSFDAWKRKPLKDPKFKAEYDALESEFALARTLIGERAKVKLT
                     QAQVARRMGTSQSAVGRMESGRAMPSTASLVKYARAVGKELKIAL"
BASE COUNT          835 a          977 c          966 g          700 t
ORIGIN      
        1 acatccgcgg ccacatcgcc gacttggaaa agcggatgcg cgaggccgcc gccgaccctg
       61 ttcgaatatc tgcccgagga cgcgctggtg ttctgcgacg aaagccatgt caccgtgccc
      121 cagatcggcg ccatgtacaa aggcgattac cggcgcaaat tcaccctggc cgaatatggc
      181 ttccgcctgc cctcttgcat cgacaaccgc ccgctcaaat tcgaggaatg ggacgcgatg
      241 cggccgcaga ccttttacgt ttcggcgacg cccggccatt gggaacttgg cgctacccgt
      301 ggcgtcttcg ccgaacaggt gatccgcccc accgggctga tcgatccgcc ggtcgaaatc
      361 cgcccggtgg aaagccaggt ggacgatctg atcgccgaat gccgcctggt ggccaagaag
      421 ggctatcgcg cgctggtcac cacgctcacc aagaagatgg cggaagacct caccgaatat
      481 atgcatgagc agggcattcg cgtgcgctac atgcattcgg acgtggaaac gctggagcgc
      541 atcgagatca tccgcgacct gcggctgggc gccttcgacg tgctggtcgg catcaacctg
      601 ttgcgcgagg ggctggacat tcccgaatgc gccctggtgg cgatcctcga cgccgacaag
      661 gaaggctttc tgcgcagcga aaccagcctg atccagacca tcgggcgcgc cgcgcgcaac
      721 gtggacagca acgtcattct ctatgcggat catgtcaccg gctcgatgca gcgcgcgatg
      781 gccgagacca cgcgccgccg cgagaagcag cacgcctaca atcaggccca cggcatcacc
      841 cccgcctcga tcaagaaaca gatcggcgac atcatgggat cgatgtacga gcgcgaccat
      901 gtgacggtgg gcgcgggcga gctgggcttc gccgaagacg gcaaggaatt catcggccac
      961 aacatccgcg gccacatcgc cgacttggaa aagcggatgc gcgaggccgc cgccgacctc
     1021 gaattcgaag aggccgcgcg gctgcgcgac gagatcaagc gcctgaccgc ggtggaactc
     1081 gccgtggccg acgacccgtt cgcgagacag tcggaagtcg aacgggccgt ggatgatgcg
     1141 tttttgaatg cggtgaggga tggctcttca cctccccctg gcggggaggt cggtcccgct
     1201 aagcgtggcc ggatgggggg taagcgtacc aaactcaaac gcgccggccg gccgaatgtg
     1261 ccgaagactt tcggcagccg ctcaaaatag cgggccgacc tgtcctccaa agcgcgcagc
     1321 gcgaaggagg aagacatttg ggagcaggag cagatatata accttctgct aacagtcgcc
     1381 cttaagcacc gaatcatgac aagatttagt gctttaaaat aaagaacaaa tggtgtacaa
     1441 agcgctctag gttagcccat gtcacaaccc tcgaccctcc gtctgctccg cgatagcaat
     1501 gcgactatgc attgcgcgga caaccttgct ttcatgcggc cactcaagga cgagagcatg
     1561 cagctcatcg ttacgtcgcc gccttacaat atcggcaaat cgtatgaggc gcgctcgtca
     1621 ctcgactctt acatacaagc ccaaacgcag gttatctcag aatgcgttcg cctccttcac
     1681 ccgcgcggtt cgctgtgttg gcaggttggc aatcatgtct tcaaaggtga gatcattccg
     1741 ctcgacatag tcctgtatcc gatctttcgc gagcatggcc taaaactacg aaaccggatc
     1801 gtctggcact ttgaacacgg cctccattgc tcaaagcgac tctcaggtcg ctacgaaaca
     1861 attctctggt tcaccaaagg cgacgattac attttcaatc ttgatgccat tcgggtaccc
     1921 tcaaagtatc ctggaaagaa gtatttcaag ggtccaaaag ccgggcagct atcctgcaat
     1981 ccgctgggaa agaacccagg tgatgtttgg gttattccca acgtaaagaa caatcacgtt
     2041 gagaagacaa ttcatccctg ccaatttccc gtcgaattga ttgaacgatt ggttcttggc
     2101 ctgaccgaac caaggggagc cgtctttgat ccctatatgg gagtaggtac cgcagtggtg
     2161 gcggcagtga aacatgggcg cgtcggttac ggctgcgacg tcgtgcagga ttatgtggac
     2221 attgcctggg agcgtgttca tgcacttaaa tctggtacgt taaagacaag gcctatgaac
     2281 aaaccgattt atgaccccaa caaaccttat ggcggccaca aaatagtacc gctgttcagg
     2341 aaaatgggat gaaactcagg atattcagct accgatacgc gcaagaaatt ttgcagcacc
     2401 cgaacaatgt ggtcgcttgg aacgaaattg agaagatact ttcaagggcg ccaatcttta
     2461 tctaccccgg caagagcaag aatcaaaaac tgcaaatcgt gcagcaatta atgaatacct
     2521 acttcgatag gcgatttgcc gttgatgcgg tttggaaata ccacccgctc gctacaaaaa
     2581 ttgcgaagtc cagtctagcc gctgactttc gcaaagagtt tggcagcatc acggtgcaag
     2641 ccgaaatcca attcggcaac atggcgcgct ggtactcaga tatatttaaa tttcagacgg
     2701 cctattcgca atctctcatt cagtgcggcc tatgcgttgt ccccacagga gagatggcta
     2761 aggcgataga ctcaaatgtc gcgcattacg agcggaccat ccgtgagcta ccttcagcca
     2821 atctttcgat aactcttcct atacttctag tcggcctgga acaggataag aggacacccg
     2881 tcgtagatgt tcgagcctgt caatttgcgt ctctcaaaga aattgtcgga aaaggcaatt
     2941 ctgaaaaccg ttggcgaatc attaatggat acgtctctgg tgcggatatg gcatcgatcg
     3001 ggccggaaag tgacaccggt ccgatgctac ttgacgagat tgatgaagcg gacaccgagg
     3061 attcagatta aggcgctact tttggcttct ccaagttcac tagttgcttt cgttcgatag
     3121 tttattgcca tgtatcaatt ttaatatgtt tcaagatggc accaccgcgc gagcttgaaa
     3181 tcgcgcttgc gcggatacag gagatacggg aatgagaagc ttcgacgcct ggaagcgaaa
     3241 gccgttgaag gaccccaagt tcaaggcgga atacgacgcg ctggaaagcg aattcgcgct
     3301 ggcccgtacg ctgatcggcg agcgcgccaa ggtgaagttg acccaggccc aggtcgcccg
     3361 ccgcatgggc acctcgcaat ccgccgtggg gcggatggag agcgggcgcg ccatgccctc
     3421 caccgcgtcg ctggtgaaat atgcccgcgc ggtcggcaaa gaactcaaga tcgcgctt
//
LOCUS       DASWPU010000099         6495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_37602,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000099 DASWPU010000000
VERSION     DASWPU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37602"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /locus_tag="VGC27_02190"
     CDS             <1..1685
                     /locus_tag="VGC27_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY0281414.1"
                     /translation="RKEAIKPGDIMILLPRREPFAGEIIRKLKDRGVPVAGADRIKLT
                     EQIAVMDLIALGRFALLPEDDLNLAALLRSPLVGLSEDDLFALCYGRKGALWHELSQG
                     RQEFPAAYEFLAGTRARADRTPPFEFYAQVLTGARQKLLARLGPETGDAIDEFLALAL
                     AYESTGTPSLEGFLHWIERGGAEVKRDMERGRNEVRVMTVHGAKGLEADIVILPDTTS
                     LPDGPAAKGHLLYTDDGVLFPLADKTAPEKVRQAKLAAQAETLKEHRRLLYVALTRAK
                     ERLIICGFENKKGIREGSWHQLAERAAEVLGTEIAQGDATIRVFGAAPVSPAASETAS
                     APETIAIPGWAHTPPAAERPSPWLIRPSAAAGMDEPVLPSPLGQNGAARFRRGLLVHA
                     MLAHLPEVERNRREDAARNFLHARGLDGAEADQLTAETLAVLDDPLFAAAFAPNSRAE
                     VAIVADLPELGAGARVHGRVDRLAVAGDRVLIVDFKTNRPPPAHEEDVPGLYATQMAL
                     YRAAAARIFPAKRIVCALVWTEGPVLMPLSEAFLAAETGRIHARLDRGVSGS"
     gene            1716..2039
                     /gene="trxA"
                     /locus_tag="VGC27_02195"
     CDS             1716..2039
                     /gene="trxA"
                     /locus_tag="VGC27_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY0281415.1"
                     /translation="MPSPIKISDASFQNDVLNAKKPVVVDFWAEWCGPCRMIAPALDE
                     LASELGEKITVAKLNIDENPNVPQKYGVRGIPTMMIFSQGQVAATKVGALPKSKIKEW
                     IESSI"
     gene            complement(2254..3567)
                     /locus_tag="VGC27_02200"
     CDS             complement(2254..3567)
                     /locus_tag="VGC27_02200"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY0281416.1"
                     /translation="MSLAGSDAVLDRLLKLHPKKIDLVLDRILRLLADLGHPERRLPP
                     VIHVAGTNGKGSVCAFSRAMLEAGGHKVHVYSSPHLVRFHERIRVAGNLISEVELMGL
                     LLECEQANEGRPITFFEITTAAAILAFARRPADALVLEVGLGGKYDATNIVARPLLTV
                     ITPVGLDHAEFLGNTLSDIAAEKAGIIKSGVPVIVGPQDDAPRDVILRRADALGAPAF
                     VFGQDFFAHQEHGRMVYQDLDGLLDLPLPRLAGRHQIENAALAIAGLRRAGGSWAKDG
                     AIEQGLRTVEWPARLQRLARGPLIDAAPEGAEVWLDGGHNPHGAAALARAMADFEERA
                     EKPLYLICGMLKTKDAAGFLSAFRGLVRHIVTVDIPGEEASLGAGALYDRARSAGLDA
                     SPAEDMEDAMMQIAAQARVAPREPPPRILICGSLYLAGQVLAENG"
     gene            complement(3702..4508)
                     /gene="accD"
                     /locus_tag="VGC27_02205"
     CDS             complement(3702..4508)
                     /gene="accD"
                     /locus_tag="VGC27_02205"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY0281417.1"
                     /translation="MNWIANFVRPRIKSLIGGRTSDTPENLWKKCPACGEMIFHRDLI
                     AAQNVCPQCGHHLRIGAPERFAALFDGAAYEELVPPEVAADPLRFRDEKRYSDRLKDA
                     RAKTGRSDALAAARGTLEGLPVMAAVQVFDFMGGSLGMAVGEALVNAMQTAATESRPF
                     ILFVASGGARMQEGVLSLMQMPRVTIAVDMLREAGLPYIVVLTDPTTGGVSASYAMLG
                     DVHIAEPGALIGFAGQRVIAQTMREQLPDGFQRAEYLLEHGMVDMVVHRH"
     gene            complement(4683..5492)
                     /gene="trpA"
                     /locus_tag="VGC27_02210"
     CDS             complement(4683..5492)
                     /gene="trpA"
                     /locus_tag="VGC27_02210"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY0281418.1"
                     /translation="MSRISVRFAELKKAGRAAFVPFVTAGDPDAETTSALLAKLPAVG
                     ADLIELGVPFSDPMADGPAIQASSLRALEAGMTLPKVLELVRKFRKADRATPLVLMGY
                     YNPIHAYGTARFAKDAADAGVDGLITVDLPPEEDEVLRLPAAAQNIDIVRLATPTTDA
                     ARLQAVLDGAGGFLYYVSIAGVTGTKSFAEGDVRTAVAKIKAASGLPCAVGFGIKTPE
                     QAAAVAQFADGAVVGSAIVERFAAAGDKGERTRDVLDFCASLAQSVHAARG"
     gene            complement(5596..>6495)
                     /gene="trpB"
                     /locus_tag="VGC27_02215"
     CDS             complement(5596..>6495)
                     /gene="trpB"
                     /locus_tag="VGC27_02215"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY0281419.1"
                     /translation="LLARRMGKTRIIAETGAGQHGVAVATVCAKFNLPCEIFMGAVDV
                     ERQKPNVFRMKLLGAKVNAVTSGAQTLKDAMNEALRDWVTNVHNTYYIIGTAAGPHPY
                     PAMVRDFQTVIGNETRAQMLEREGRLPDLVSACVGGGSNAIGMFHPFLDDPDVELHGV
                     EAAGEGADTPRHAATLSKGTPGVLHGARSYLLQSRDGQIQEAHSISAGLDYPGVGPEH
                     SWLKDQGRVKYFSVTDQEALVAFVALSKLEGILPALESAHAVAHIMRVAKQMAKDKLI
                     AICLSGRGDKDAASVAAALGEEI"
BASE COUNT         1131 a         2131 c         2178 g         1055 t
ORIGIN      
        1 accgcaagga ggcgatcaag ccgggcgaca ttatgatttt gctgccgcgg cgcgaaccgt
       61 tcgccggcga gatcatccgc aagctgaagg atcgcggcgt gccggtggcg ggcgccgacc
      121 gcatcaagct gaccgaacag atcgcggtga tggacctgat cgcgctgggc cgctttgcgc
      181 tgctgccgga agacgatctc aatctggcgg cgctgctgcg ctcgccgctg gtcgggctga
      241 gtgaagacga cctcttcgcg ctgtgttacg ggcgcaaggg cgccctgtgg cacgaacttt
      301 cgcagggacg gcaggagttc ccggcagcat acgaatttct cgccggaacg cgggcgcggg
      361 ccgaccgcac gccgccgttc gaattctatg ctcaggtcct gaccggggcc cgccagaagc
      421 tgctggcgcg gctcggcccg gaaacgggcg acgccatcga cgagttcctg gcgctggcgc
      481 tggcttatga gagcacaggc acgccgtcgc tggaaggttt cctgcattgg atcgagcgcg
      541 gcggcgccga ggtcaagcgc gacatggaac gcggccgcaa cgaggtgcgg gtgatgaccg
      601 tgcatggcgc caaggggctg gaagccgaca tcgtcatcct gcccgatacc acctcgctgc
      661 ctgacggccc ggcggccaag ggacatcttc tgtacacgga cgacggcgtg ctgttcccgc
      721 tcgccgacaa gacggcgccg gaaaaggtga ggcaggcgaa gctggcggcg caggccgaaa
      781 ccctcaagga gcaccgccgg ttgctgtatg tggcgctcac ccgcgccaag gaacggctca
      841 tcatctgcgg cttcgaaaac aagaagggca tccgcgaagg ctcctggcac cagcttgcgg
      901 aacgcgcggc ggaggtgctg ggcacggaaa tcgcgcaagg cgacgccaca atccgcgtct
      961 tcggcgccgc cccggtttcc cccgccgcga gcgaaacggc gagcgccccg gaaacaatcg
     1021 caattcccgg ttgggcacac acaccgcccg cggcggagcg tccgtcgccc tggctcatcc
     1081 gcccgtccgc ggcggcgggc atggatgaac cggtgctgcc ctcgccgctg ggacagaatg
     1141 gcgcggcgcg attccgccgg gggcttctgg ttcacgccat gctggcgcac ctgcccgaag
     1201 tggagcgcaa ccggcgcgag gacgcggcgc gcaacttcct gcacgcgcgc ggacttgacg
     1261 gcgcggaagc ggaccaactg acagcggaga cattggccgt tctcgacgat ccactgttcg
     1321 ccgccgcctt cgcacccaat agtcgcgccg aagtcgccat cgtcgccgat ctgccggaat
     1381 tgggcgccgg cgcgcgcgtc catgggcgcg tggaccgctt ggctgtcgcc ggtgatcgcg
     1441 tccttatcgt ggatttcaaa acgaaccgcc cgccgcctgc ccacgaggaa gacgtgcccg
     1501 gactctatgc aacgcaaatg gccctatatc gcgctgcggc agcaaggata ttcccggcaa
     1561 agcgaatcgt ctgcgcgctg gtctggaccg aaggcccggt tctgatgccg ctgagcgagg
     1621 cctttctggc ggcggaaacg ggccgcatcc acgcgcgcct tgaccggggg gtgagcggtt
     1681 cctagatata gtccgcctga tgtgaggata tgcgtatgcc ttcgccgatc aaaatctccg
     1741 acgcgtcgtt ccagaacgac gtcctgaatg cgaaaaagcc ggtcgtggtg gatttctggg
     1801 ccgaatggtg cgggccttgc cgcatgatcg cgcccgcgct tgacgaactg gcgtcggaac
     1861 tcggcgaaaa aatcacggtc gcgaagctca atatcgacga gaatcccaac gtgccgcaga
     1921 aatacggcgt gcgcggcatc cccacgatga tgatcttcag ccagggccag gtggcggcga
     1981 ccaaggttgg cgcgctaccc aagtcgaaga tcaaggaatg gatcgaaagc tcgatctgaa
     2041 ggccgctcag ccaattcaaa ttgattgtca ttcccggccg agcatcggtc cggagcgtta
     2101 gcgtacggac gcgatgcgag gggaagggaa tccaggcttc accataccga gacgcatcga
     2161 accctggatc cctttccctc gcgcttcgcg ctcgccgggg atgacagttg cgatttgttt
     2221 tctccctcgt aaacggtgga ggaaagaagc gattcacccg ttctccgcca acacctgtcc
     2281 cgccagataa agcgagccgc aaatgagaat gcgcggcggc ggttcgcgcg gcgccacgcg
     2341 cgcctgcgcc gcgatctgca tcatggcgtc ttccatgtcc tcggccggcg aagcgtcgag
     2401 accggcggaa cgcgcgcggt cgtataaggc gccggcgcca aggctcgcct cttcgccggg
     2461 aatatcgacg gtgacgatgt ggcggaccag tccgcggaac gccgacagga aaccggcggc
     2521 atccttggtc ttcagcattc cgcagatgag atagagcggc ttttccgcgc gttcctcgaa
     2581 atcggccatg gcgcgcgcca gcgccgcggc gccgtgcgga ttgtggccgc cgtcgagcca
     2641 gacttcggca ccctccggcg cggcgtcgat caacggaccg cgcgccaagc gctgcaagcg
     2701 cgccggccac tcgacggttc tcagcccctg ttcgatggcg ccgtccttgg cccagcttcc
     2761 gccggcgcgc cggagcccgg cgatcgccag cgcggcgttc tcgatctgat gacgcccggc
     2821 cagccgcggc aaaggcagat cgagcaggcc gtcgaggtcc tgatagacca tgcggccgtg
     2881 ttcctgatgg gcgaagaaat cctgtccgaa cacgaaggcc ggcgcgccca gcgcatcggc
     2941 gcggcgcagg atgacgtcgc gcggcgcatc gtcctgcggg ccgacgatca cgggtacgcc
     3001 ggatttgata atgccggcct tctcggcggc gatgtcggac agcgtattgc cgaggaactc
     3061 ggcgtgatcc aggccgaccg gcgtgatgac ggtgagaagc gggcgggcga cgatgttggt
     3121 cgcgtcgtat ttgccgccga ggcccacttc cagcaccagc gcatcggccg gacggcgcgc
     3181 gaaggccagg atcgccgcgg ccgtggtgat ctcgaaaaaa gtgatgggcc ggccttcatt
     3241 cgcctgctcg cactccaaga ggaggcccat cagttccact tcgctgatca aattgccggc
     3301 cacgcggatg cgctcgtgga agcgcaccag atgcggcgag gaataaacgt gaaccttgtg
     3361 cccgcccgct tccagcatgg cgcgggagaa ggcgcagacc gaacccttgc cgttggtgcc
     3421 ggcgacgtgg atcacgggcg gcaggcggcg ctcgggatgg ccgaggtccg ccagcagccg
     3481 gagaatccgg tccagcacca gatcgatctt cttgggatgg agcttgagaa gccggtcgag
     3541 aaccgcgtcg ctcccggcca ggctcatgtg gcgcttccgg ccggaggtgc cggaacctga
     3601 gaagccgtgc ttgcggcgat caaggctggc gatgcgggcc gagtcttagg ccgcgggcgt
     3661 ttcatcaaga gatggacgac gcgcgacagc gtctcgcgca gctagtggcg atggacgacc
     3721 atgtccacca tgccgtgttc gagcagatat tcggcgcgct ggaaaccgtc cggcagctgt
     3781 tcgcgcatgg tctgggcgat gacgcgctgc ccggcgaagc cgatgagcgc gcccggctcg
     3841 gcgatatgca cgtcgcccag catggcatag gaggcgctga cgccgccggt ggtcggatcg
     3901 gtgagcacga cgatataagg cagtcccgcc tcgcgcagca tatccacggc gatggtcacg
     3961 cgcggcatct gcatcagcga cagcacgccc tcctgcatgc gcgccccgcc cgaggcgacg
     4021 aacaggatga acggccggct ttccgtcgcc gccgtttgca tcgcgttgac caacgcctcg
     4081 cccacggcca tgccgagcga gccgcccatg aagtcgaaga cctgcaccgc cgccattacc
     4141 ggcagccctt ccagcgtgcc gcgcgcggcg gccagcgcat cgctgcggcc ggtcttggcg
     4201 cgggcatcct tcaagcggtc gctgtagcgc ttctcgtcgc ggaagcgcag cgggtcggcg
     4261 gcgacttccg gcggaaccaa ctcctcatag gccgcgccgt cgaacaacgc cgcgaagcgc
     4321 tccggcgcgc cgatgcgcag atgatgaccg cattgcgggc agacgttctg cgccgcgatc
     4381 agatcgcgat ggaagatcat ctcgccgcag gcgggacatt tcttccacag gttctccggc
     4441 gtgtcggacg tgcgcccgcc gatcaggctc ttgatccgtg gacgaacgaa attggcgatc
     4501 cagttcatgg cgtcatccta gaccaggacg gctgcaaata gaacgtctgt cattcccggc
     4561 gagcgcgaag cgcgagggaa gggaatccag aaacctgcgc aaactcttgg attcccttcc
     4621 cctcgctccc tgcggtcgct cggccgggaa tgacaagttg cctaaaacca tcttaacacg
     4681 cctcaccccc gcgccgcatg taccgattgc gccagggaag cgcagaaatc cagcacatcg
     4741 cgggtgcgtt cgcccttgtc tcccgccgcc gcgaagcgtt cgacgatggc cgagcccacc
     4801 acggcaccat ccgcgaattg cgccaccgcg gccgcctgtt cgggcgtctt gatgccgaag
     4861 ccgacggcgc aaggcagccc gcttgcggct ttaatcttcg ccaccgccgt gcgcacgtcg
     4921 ccttcggcga agctcttggt gccggtgacc ccggcgatcg agacgtaata gagaaagccg
     4981 cccgcgccat cgagcacggc ttgcaggcgc gccgcatcgg tcgtcggcgt ggccagccgc
     5041 acgatgtcga tgttctgggc ggcggccggc aggcgcagca cttcgtcttc ctccggcggc
     5101 aggtccaccg tgatcagccc gtccacgccc gcatcggccg cgtccttggc gaagcgcgcg
     5161 gtaccgtagg catggatcgg attgtaatag cccatcagga ccagcggcgt cgcccggtcc
     5221 gccttgcgga acttgcgcac cagttccagc accttcggca aggtcatgcc ggcttcgagc
     5281 gcgcgcaagg acgatgcctg gatcgcgggg ccgtcggcca tcggatcgga gaacggcacg
     5341 ccaagctcga tcagatcggc gccgacggcg ggcagcttgg ctagcaatgc cgaagtcgtt
     5401 tccgcgtcgg gatctccggc cgtgacgaac ggcacgaagg cggcgcggcc cgcctttttc
     5461 aattcagcaa aacgaacgga gattctgctc atgaggacct accgggaaag tcatgcacgc
     5521 ggaggcgcgg agaacgcgga gcggtcttcc actcttctcg actccgcgtc tccgcgtgag
     5581 cgaattcttt gttgttcata tctcttcccc cagcgcggcg gcgacggagg cggcgtcctt
     5641 gtcgccgcga ccggacagac agatggcgat cagcttgtcc ttcgccattt gcttcgccac
     5701 gcgcatgatg tgggcgacgg catgggccga ttcaagcgcc ggaaggatgc cttcgagctt
     5761 cgatagcgcc acgaaggcga ccagcgcctc ctggtcggtg acggagaaat atttcacccg
     5821 gccctgatcc ttgagccagg aatgttccgg gccgacgccg gggtaatcga gcccggcgga
     5881 gatcgaatgc gcctcctgga tttggccgtc gcggctttgc agcaggtagg agcgcgcgcc
     5941 gtgcagcacc ccgggcgtgc ccttggacag cgtggccgcg tggcgcggcg tatcggcgcc
     6001 ctcgcccgcc gcctcgacgc catggagttc gacatcggga tcgtcgagga agggatggaa
     6061 catgccgatg gcgttggagc cgccgccgac gcaggccgag accaggtcgg gcaggcggcc
     6121 ttcgcgctcc agcatctggg cgcgggtttc gttgccgatc accgtctgga aatcgcgcac
     6181 catggccgga tagggatgcg gaccggcggc cgtgccgatg atgtagtagg tgttgtgcac
     6241 gttggtgacc cagtcgcgca gcgcctcgtt catggcatct ttcagcgtct gcgcgccgga
     6301 ggtgacggcg ttgaccttgg cgcccagcag cttcatgcgg aacacgttgg gcttctgccg
     6361 ctcgacgtcg acggcgccca tgaagatctc gcacggcagg ttgaacttgg cgcacacggt
     6421 ggcgacagcg acgccgtgct ggccggcgcc ggtctcggcg atgatacgcg tcttgcccat
     6481 gcgccgggcg agcag
//
LOCUS       DASWPU010000100         4336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_92495,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000100 DASWPU010000000
VERSION     DASWPU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92495"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VGC27_02220"
     CDS             <1..207
                     /locus_tag="VGC27_02220"
                     /inference="COORDINATES: protein motif:HMM:NF023522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HEY0281420.1"
                     /translation="LEQRMKVTVYILDRHLRADGIRVAVFRQIRNGESWIDAPVASDT
                     ATKLTDAILTRAREMRLATQAPAN"
     gene            266..2287
                     /gene="leuS"
                     /locus_tag="VGC27_02225"
     CDS             266..2287
                     /gene="leuS"
                     /locus_tag="VGC27_02225"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY0281421.1"
                     /translation="MARYNAKDSEKKWQEAWNRSGTFLTPTDSTRPKCYVLEMFPYPS
                     GRIHIGHVRNYTMGDVIARFKRAQGFNVLHPMGWDAFGLPAENAAREKGVSPRDWTYA
                     NIAAMKDQLKLMGLSIDWNREFATCDPAYYRHQQKLFLDFHRDGLVYRSEADVNWDPV
                     DQTVLANEQVIDGKGWRSGAPVERRKLSQWFFKITAFSDDLLTALDRLERWPEKVRLM
                     QKNWIGRSEGMRFAFDLSNGKKVDVFTTRQDTLFGASFVALSPDHPLTGELAGADPKL
                     KAFVAECRRIGTAEEAIEKAEKLGYDTGLRATHPFDPDWKLPVVVANFVLMAYGTGAI
                     FGCPAHDQRDLEFARKYKLPVRPVVVPEDADPATFEIGDEAYIGPGRLANSRFLNGLS
                     VEAAKEEVAGRLEAEGRGERTVNYRLRDWLVSRQRPWGCPIPMVHCPQCGVVPAKESD
                     LPIKLPDDLAFNVKGNPLAAHPTWKYTVCPKCGGEAVRDTDTLDTFVDSSWYFARFCD
                     TKAEQPVNRAAVDYWLPVDQYIGGIEHAILQLLYARFFLRAMKKAGYVKLDEPFAGLF
                     TQGMVCHETYKDADGNWLSPDEVEKRGGQAFCKGTGEPVHVGPSEKMSKSKKNVVAPE
                     QIIDIFGADTIRWFMLSDTPPERDIEWTDEGAEGCWRFVQRVWRLVSEA"
     gene            2342..2806
                     /locus_tag="VGC27_02230"
     CDS             2342..2806
                     /locus_tag="VGC27_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY0281422.1"
                     /translation="MRRAAHRAIAAVTDELASLRFNRAVAQIYAFVNAISAAEVSGAA
                     RREALETLVLLIGPMMPHLAESCWEALGHRQFVAMTPWPVADDALVAAETITIAVQVN
                     GKLRGTIEAAPGTGKDAMEKNALALDTVKRALDGKTPRKVIVVPDRIVNIVA"
     gene            2803..3336
                     /gene="lptE"
                     /locus_tag="VGC27_02235"
     CDS             2803..3336
                     /gene="lptE"
                     /locus_tag="VGC27_02235"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY0281423.1"
                     /translation="MRAAGTIAVLGLALGLYGCGFHPLYAIPGAPAGAMRQELGAIYV
                     EPIPDRLGYELRNALIDVLDARTQAAGATYRLRLTLAEKSDAVGVQSQTVGAVTQTAI
                     TRYNDKLTVGYELVDVASGAVINRGTESGLSAYNVLSSPYATLVAQQDAARRAADDIA
                     DRIRIDLAVFFAQNTKK"
     gene            3333..>4336
                     /gene="holA"
                     /locus_tag="VGC27_02240"
     CDS             3333..>4336
                     /gene="holA"
                     /locus_tag="VGC27_02240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY0281424.1"
                     /translation="MIVKASDADRTAKNPPRGLVAALVFGPDSGLIRERAETLLKTVV
                     DDLADPFRVSDLDEQTLANDPARLFDEAASISMLGGRRVVRVRGAGNGLAKLFESFLA
                     EPAGDALVVVESGDLAKGAALRRLFEEADNAAAIPCYADSARDLSDVVRDALRAEGIA
                     IAPDALADAVSRLGSDRGVTRRELEKLILYARGRKQVTLEDVRAIMGDEAEARIEEAC
                     DAAGTGDFVRLDLALERLWTADTAPVAVLRQAMSHFQRLLLVGAESLRGESVDGAMRK
                     LRPPVHFSRAASFKAQAQKWNEAKLFEALDLLLETEALCKTTGVPAEAVCGRALFNVA
                     A"
BASE COUNT          777 a         1424 c         1414 g          721 t
ORIGIN      
        1 ctcgaacagc gcatgaaagt cacagtctat attctggacc ggcacctgcg cgccgacggg
       61 atcagggtcg cggtcttccg tcagatccgc aacggcgaat cctggatcga cgccccggtt
      121 gcttccgaca ccgccaccaa gctgaccgat gccattttga cgcgcgcccg cgaaatgcgc
      181 ctggcgaccc aggccccggc caactaaggc gcgcaaagcg cgccgggcat tttgcctgat
      241 tgcgctctag ggattcgaaa ctcgcatggc gcgctacaac gccaaggact cggaaaagaa
      301 gtggcaggag gcctggaacc gttccggcac cttcttgacg ccgaccgatt ccacccgccc
      361 caaatgctat gtgctggaga tgttccctta tccgtcgggg cgcatccata tcggccatgt
      421 acgcaactat acgatgggcg acgtcatcgc ccgcttcaag cgcgcgcaag gcttcaacgt
      481 gttgcacccg atgggctggg acgcgttcgg gcttccggcg gaaaacgccg cgcgcgagaa
      541 gggcgtatcc ccgcgcgact ggacctacgc caacatcgcg gcgatgaagg atcagctcaa
      601 gctgatgggc ctgtcgatcg attggaaccg cgaattcgcc acttgcgatc cggcctatta
      661 ccgccaccag cagaaactgt tcctggattt tcaccgcgac ggcctggtct atcgcagcga
      721 agccgacgtc aactgggatc cggtcgatca gaccgtgctc gccaacgagc aggtgatcga
      781 cggcaagggc tggcgttcgg gcgcccccgt cgaacggcgc aagctgtcgc aatggttttt
      841 caagatcacc gccttctccg acgatctgct gaccgcgctc gaccggctcg aacggtggcc
      901 ggaaaaagtc cggctgatgc agaagaactg gatcggccgt tccgagggca tgcgctttgc
      961 cttcgatctg tcgaacggga agaaagtgga tgtcttcacg acgcgccagg acaccttgtt
     1021 cggcgccagc ttcgtggcgc tgtcgcccga tcatccactg accggggaac tggccggggc
     1081 cgatcccaag ctgaaagcct tcgtcgccga gtgccggcgc atcggcaccg ctgaggaagc
     1141 gatcgagaaa gccgaaaagc tgggctacga caccggcctt cgcgcgaccc atccgttcga
     1201 cccggactgg aaattgccgg tcgtggtcgc caatttcgtg ctgatggcct acggcaccgg
     1261 cgccattttc ggctgccccg cccacgacca gcgcgacctg gaattcgccc gcaaatataa
     1321 actgccggtg cggcccgtcg tggtgcccga agacgcggac cctgcaacct tcgagatcgg
     1381 cgacgaagct tatatcggcc ctggccggct ggccaattcg cgcttcctca acgggctttc
     1441 ggtcgaggcg gcgaaggaag aagtcgcggg gcgcctggaa gcggagggcc gcggcgagcg
     1501 tacggtcaat taccgcctgc gcgactggct ggtgtcgcgc cagcgccctt ggggctgccc
     1561 gatcccgatg gtgcattgcc cccaatgcgg cgtggtaccg gcgaaggaat ccgatctgcc
     1621 gatcaagctg cccgacgatc tggcgttcaa cgtcaaaggc aatccgctcg ccgcccatcc
     1681 gacctggaag tacacggtct gccctaaatg cggcggcgag gcggtgcgcg acaccgacac
     1741 gctcgacact ttcgtggatt cctcctggta tttcgcgcgc ttctgcgaca ccaaggcgga
     1801 acagccggta aaccgcgccg cggtcgatta ctggctgccg gtcgatcagt atatcggcgg
     1861 catcgagcac gcgatcctgc agctgctcta tgcgcgcttc ttcctgcgcg cgatgaaaaa
     1921 ggcgggctac gtcaagctcg acgagccttt cgcgggcctc ttcacccagg gcatggtctg
     1981 ccacgagacc tacaaggacg cagacggtaa ctggctgtcg cccgacgagg tggaaaagcg
     2041 cggcgggcag gcgttttgca aaggcaccgg cgagccggtc catgtcggcc cctccgagaa
     2101 aatgtccaag tcgaaaaaga acgtggtcgc gcccgaacag atcatcgaca ttttcggggc
     2161 cgacacgatc cgctggttca tgctttcgga cacgccgccc gaacgcgata tcgaatggac
     2221 cgatgaaggc gccgaaggct gctggcgctt cgtccagcgc gtctggcggc tggtgagcga
     2281 ggcctagggc cggccgccgc ccggcacccc ggccgcgaag gcggaaggtg cggcgctgga
     2341 gttgcgccgg gccgcccatc gcgccatcgc ggcggtgacg gacgagctgg cctccctcag
     2401 attcaatcgc gcggtggcgc agatttatgc cttcgtcaat gctataagcg ccgccgaggt
     2461 ctcgggcgcg gcgcgccgcg aggctttgga gactctggtg ctgttgatcg gtccgatgat
     2521 gccgcatctg gcggaaagct gctgggaagc cttgggacac cggcaattcg ttgccatgac
     2581 gccctggccc gtggccgacg atgcgctggt cgccgccgaa acgatcacca tcgcggtgca
     2641 ggtcaacggc aagctgcgcg gcaccatcga agcggcgccc ggcaccggca aggacgcgat
     2701 ggaaaagaac gcgctggcgc tcgacaccgt gaagcgcgcg ctcgacggca agactccgcg
     2761 caaggtgatc gtcgtgccgg atcgcatcgt gaacatcgtc gcatgagggc cgccgggacc
     2821 atagccgttc tcggccttgc gcttggcctt tacggctgcg gctttcatcc gctgtacgcg
     2881 attcccggcg ccccggccgg cgccatgcgc caggaattgg gcgcgatcta tgtcgagccg
     2941 attcccgacc gtttgggtta cgagcttcgc aacgcgctca tcgacgtgtt ggacgcgcgc
     3001 acccaagccg ccggcgctac ctatcgcctg cgcctgacgc tggcggaaaa gagcgacgcc
     3061 gtcggcgtgc aaagccagac cgtcggcgcc gtcacccaga ccgccatcac ccgctacaac
     3121 gacaagctga ccgtcgggta cgaactcgtc gacgtggcga gcggggcggt catcaaccgc
     3181 ggcacggaaa gcggcctttc ggcttacaac gtgctgtcct cgccatacgc cacgctggtg
     3241 gcgcagcagg atgccgcccg gcgcgccgcc gacgacatcg ccgaccgtat ccgcatcgat
     3301 ctcgccgtct tcttcgcgca aaacacgaaa aaatgatcgt caaagcgtcc gacgccgacc
     3361 gcaccgcgaa aaacccgccg cgcgggcttg tcgccgcgct ggtgttcggg cccgacagcg
     3421 gactgatccg cgagcgcgcc gaaaccctct tgaaaaccgt ggtggacgat ctcgccgatc
     3481 cgttccgcgt ttccgatctc gacgagcaga ccttggcgaa cgacccggcg cgcctgttcg
     3541 acgaagcggc gtcgatctcg atgctgggcg gacggcgcgt ggtgcgcgtg cgcggcgcgg
     3601 ggaacggcct ggccaagctg ttcgaatcct tcctggccga gccggccggc gacgcgctgg
     3661 tggtggtgga aagcggcgat ctcgccaagg gcgcggcgct gagaagattg ttcgaggaag
     3721 ccgataacgc ggcggcgatc ccctgttatg ccgattcggc gcgcgatctt tccgacgtcg
     3781 tgcgcgacgc cttgagggcc gaagggattg ccatcgcccc ggacgctttg gcggatgcgg
     3841 tgtcgcgcct gggttcagat cgcggcgtga cccggcgcga actggaaaag ctgattttat
     3901 acgcgcgcgg tcgcaagcag gtcaccctcg aggacgtgcg cgccatcatg ggcgacgagg
     3961 ccgaggcgcg catcgaggaa gcctgcgacg cggcggggac gggcgatttc gtccgcctcg
     4021 atctggcgct cgaacggctg tggacggcgg acacggcgcc ggtggcggtc ctgcgccagg
     4081 ccatgtcgca tttccagcgc ctgctgctgg tcggcgccga aagcctgcgc ggcgaaagcg
     4141 tggacggggc aatgcgcaag ctgcgcccgc cggtgcattt ttcgcgcgcc gcgtccttca
     4201 aagcccaggc ccagaaatgg aacgaggcca aactcttcga ggcgctcgat ctgctcttgg
     4261 aaaccgaggc tttgtgcaag acgacgggcg ttcccgccga ggccgtctgc ggccgcgccc
     4321 tgttcaacgt cgccgc
//
LOCUS       DASWPU010000101         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_110889,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000101 DASWPU010000000
VERSION     DASWPU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110889"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1080
                     /gene="lepB"
                     /locus_tag="VGC27_02245"
     CDS             238..1080
                     /gene="lepB"
                     /locus_tag="VGC27_02245"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY0281425.1"
                     /translation="MKFDPAEWRSRASDLAQKAAAPFVAFFSIKEVRAAIDWLKEPVL
                     AVALVFAATTAIAQPFYVPSGSMQPTLAIGDLTLATKFNYGYSRYSIPYVSGPTPSGR
                     FLGRMPTVGDVAVFRLPSQTSVTYVKRLIGMPGDRIQMRQGRLWINGQELKLREAGTD
                     QAENGPNELGAGEYVAVAKYIETLPNGVEHPIFKKRWDGPYDNTPVYVVPDGYFFMMG
                     DNRDDSSDSRVPPSFGGVGYVPYGNLVGRVFVVVASVDFTNADAIWEWVTEFRFSRVL
                     TGVR"
     gene            1198..1476
                     /locus_tag="VGC27_02250"
     CDS             1198..1476
                     /locus_tag="VGC27_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0281426.1"
                     /translation="MGEALFESSARRDCGEDRFVAVGDFRGEFYMEVVENLKAKKRGR
                     PKLEHPKVQVTLRLDADVVGVFKEDGPGWQGRINEELKKTVKRKRSRS"
BASE COUNT          353 a          501 c          527 g          316 t
ORIGIN      
        1 tggcggtgaa atcgggcgcc tgcgcgccta ctgccagcgc agcgtgcgcc ggaatcgcgg
       61 caaggccgaa aagcaaggca agaccgggta tcagcgactt catgggaacc tcctggcagg
      121 ccctgattat gatccggccc aagcaagctg tcttcccaaa acagcggggt ttgtgcgagg
      181 acttgaaatc gggacaaata tccccgaaat ccgtatggtc aagaaaaggc acagcgcatg
      241 aaattcgatc cggcggaatg gcggtcgcgg gcgagcgatc tggcccaaaa ggcggccgca
      301 cccttcgtcg cctttttctc catcaaggaa gtgcgggccg ccatcgactg gctgaaagag
      361 ccggtcctgg ccgttgccct cgtcttcgcc gccacgacgg cgatcgccca gcccttctac
      421 gtgccctccg gctcgatgca gccgacgctt gccatcggcg atctgaccct ggcgacgaag
      481 ttcaactacg gctacagccg ctattcgatc ccttatgtga gcgggcccac gccctccggc
      541 cggttcctgg gccggatgcc gacggtcggc gacgtggccg tcttccgtct gccgagccag
      601 accagcgtaa cctacgtaaa acggctgatc ggcatgccgg gcgaccgtat ccagatgcgc
      661 caaggccggc tgtggatcaa cggacaagaa ttgaagctgc gcgaggcggg aaccgaccag
      721 gccgaaaacg gccccaacga attgggggcg ggcgaatacg tcgccgtcgc gaaatacatc
      781 gaaacgctgc ccaacggcgt cgagcatcct attttcaaga agcgctggga cggcccctac
      841 gacaacacgc cggtgtacgt cgtgccggac ggttactttt tcatgatggg agacaaccgc
      901 gacgattctt ccgacagccg tgtgccgccc tctttcggcg gcgtcggcta tgtgccttac
      961 gggaaccttg tcgggcgggt attcgtggtc gtcgcctcgg tcgatttcac caacgccgac
     1021 gcgatctggg agtgggtcac cgaattccgc ttctcgcggg tcctgacggg cgtgcgatag
     1081 ccccccgcct gctttacatt taaagagacg gctcatggaa tttgagtggg acgaagccag
     1141 gcggcgaagc aacctggaaa agcacgggtt ggatttcgcc gatttggata cgttcgattg
     1201 ggagaagcgc tgttcgaatc ctcggcgcga cgagattgtg gcgaagaccg cttcgtcgcg
     1261 gtcggcgatt tccgtgggga gttttacatg gaggttgttg agaacctcaa agcgaagaaa
     1321 cgcggccggc cgaagctcga acaccccaaa gttcaggtga cgctacggct cgacgccgat
     1381 gtggtcggtg tcttcaagga agacggtccc ggctggcagg gccgtatcaa tgaagaattg
     1441 aagaagacgg tgaagcgaaa acgcagccgc tcctaaccgc ccgcgacaca ggggcagcag
     1501 ccggaatatt gcgccctctc ccttgcccat gctaaggcca gcgcacgcat gaaacgggat
     1561 tcgtccgatc tggcgaattt catcgctgta tcaaatggtt attgatggtc tgccgagcgt
     1621 tttccggtga catccatgaa atggagcatg cttggcagac gaagctcaca tgtccgggat
     1681 tgcagggcgc tacgccc
//
LOCUS       DASWPU010000102         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_43733,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000102 DASWPU010000000
VERSION     DASWPU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43733"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /gene="rplJ"
                     /locus_tag="VGC27_02255"
     CDS             complement(<1..354)
                     /gene="rplJ"
                     /locus_tag="VGC27_02255"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY0281427.1"
                     /translation="MEKARKAEVVEELQSVLAKSGSVVVAHYTGMTVAQMSDLRGRMR
                     ANGASFKVAKNRLVMRALKGTPVEGISHLFKGPTGIAYSDDPISASKTAIAYAKDNDK
                     LVILGGSMGTTMLDAN"
     gene            complement(720..1430)
                     /gene="rplA"
                     /locus_tag="VGC27_02260"
     CDS             complement(720..1430)
                     /gene="rplA"
                     /locus_tag="VGC27_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY0281428.1"
                     /translation="MATSKRYKKAIEGVDANKAYTVEEAVKLIKSRATAKFDETIELS
                     LNLGVDPKHADQMVRGVVSLPSGTGRSLRVAVFAKGAKAEEARKAGADIVGAEELAAE
                     VTAGKIDFDRCIATPDMMGIVGRLGKVLGPRNLMPNPKVGTVTMDVTQAIKDAKGGAV
                     EFRVEKAGIVQAGVGKASFTEDALIQNVKTFVDSVLKARPPGAKGTYMKKISISSTMG
                     TGVKIALGSVGAASAEAH"
     gene            complement(1436..1540)
                     /locus_tag="VGC27_02265"
     CDS             complement(1436..1540)
                     /locus_tag="VGC27_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281429.1"
                     /translation="MAQAKMKDLSANDIENAMLLIEGSARSMGITVRD"
BASE COUNT          242 a          528 c          452 g          330 t
ORIGIN      
        1 gttcgcgtcg agcatggtcg ttcccatcga accgccgagg atcacgagct tgtcgttatc
       61 cttggcgtag gcgatcgccg tcttcgaggc cgagatcgga tcgtcgctgt aggcgatgcc
      121 ggtcgggcct ttgaagaggt ggctgatccc ctctacgggc gtacccttca gagcgcgcat
      181 gacaagacgg ttcttcgcca ccttgaacga ggccccgttg gcgcgcatgc gcccacggag
      241 gtcgctcatt tgggcaaccg tcatgccggt atagtgggcg acaaccacgg agccggattt
      301 cgctaagacg ctctgcagct cctcgacgac ttccgccttt ctggccttct ccacttggct
      361 ctcctttttc gatggcctac cgcattgctg cttgaggcca cccacaaacg gacaagcggg
      421 acgatccgcc tgttcccctg ttgctcattc cccgcccttc cggacggggc ctgccccagg
      481 gttcaaaccc gaacggcgca aaggctgacc tttgcgaatt tcggtcctca ccccgtctgc
      541 gctggaacat ttaagtgccg aagcactcca gcggtctcgg acaggtatcg ggtcatcgcc
      601 gacccgaaac tcttactttt ttcccgccgc ccttcggggc cgcttcgcgg cacctcaggg
      661 tgacgaaact catacgcgtc atgctgaggt gcccggcata gccgggcccc gaagcacgac
      721 tagtgcgctt ccgcgctcgc agccccgacc gaacccagcg cgatcttcac gcccgtgccc
      781 atcgtcgagg agatggaaat cttcttcata taggtgcctt tggcgcccgg cggccgcgcc
      841 ttcaacacgc tgtccacgaa cgtcttcacg ttctggatca gcgcgtcttc ggtgaagctc
      901 gccttgccga cgccggcctg cacgatgccc gccttttcaa cgcggaactc gaccgcgccg
      961 cccttggcat ccttgatcgc ctgggtcacg tccatcgtga ccgtgccgac cttggggttg
     1021 ggcatcaggt tgcgcgggcc cagcactttg ccgagacggc cgacgatacc catcatgtcc
     1081 ggggtggcga tgcagcggtc gaaatcgatc ttgcccgccg tgacttcagc cgccagttcc
     1141 tcggcgccga cgatatcggc gcccgccttc ctggcttctt ccgccttggc gcccttggcg
     1201 aacaccgcga cgcgcagcga acggccggtg cccgaaggca gcgaaaccac gccgcgcacc
     1261 atctggtcgg catgcttggg gtccacgccc aggttgagcg acagttcgat ggtctcgtcg
     1321 aacttggcgg tggcgcgcga cttgatcagc ttcaccgctt cttcgaccgt gtaggccttg
     1381 ttggcgtcaa cgccttcaat ggccttcttg taacgcttgg aggttgccat ggcggttagt
     1441 ccctcaccgt gatgcccatc gagcgggcgg agccctcgat caggagcatc gcgttttcga
     1501 tgtcgttggc gctcagatcc ttcatcttgg cctgggcgat ctcgcgaacc tg
//
LOCUS       DASWPU010000103         5011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_49597,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000103 DASWPU010000000
VERSION     DASWPU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49597"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1131)
                     /locus_tag="VGC27_02270"
     CDS             complement(280..1131)
                     /locus_tag="VGC27_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY0281430.1"
                     /translation="MMLSKGFARGFVPLCAVLSMLALSGCSLFGGSDADKPKEATYQE
                     RPIDQIYSDAWRRVRGGDWSQAAQQFAEVERQHPYSVWARRASLMSAFCYYQANKYSD
                     AITAADQYISLHPGSKEVSYAFYLKAVSLYEQIVDVGRDQSNTEAALVALQDVVQRFP
                     DSEYARDATLKIDLTLDHLAGKEMEVGRYYLYQGDYIGAINRFRVVVEQYQKTSQIAE
                     ALERLTEAYYALGITKEAQTAAAVLGANYPGSPWYESAYNLLKGHDLKPEEDKGSWIS
                     RAFNKIL"
     gene            complement(1427..2281)
                     /gene="lpxC"
                     /locus_tag="VGC27_02275"
     CDS             complement(1427..2281)
                     /gene="lpxC"
                     /locus_tag="VGC27_02275"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY0281431.1"
                     /translation="MPMRHTIAREVAAEGITLHAGTQVRMMLAPAKPGTGIVFRRADL
                     GGAEIPARYDAVSGTNLGTMLATARANVGVVEHLMAAVAGAGIDDLLVILDGPEPPIL
                     DGDALSYLDLIGKAGVKKQPAPRSAIKVLTRVEAVHKDASAALVPADALSFDFEIAFP
                     SAAIGRQSFSFPFSPDGFRREIAPARTFGFLQELERLKQIDRGHGASLDNTLAIDGDR
                     IVNASLMRFPDEFVRHKILDAIGDMALAGAPLIARFEGVRSGHALNNALLRALFADPA
                     NYRMISFS"
     gene            complement(2445..3968)
                     /gene="ftsZ"
                     /locus_tag="VGC27_02280"
     CDS             complement(2445..3968)
                     /gene="ftsZ"
                     /locus_tag="VGC27_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY0281432.1"
                     /translation="MAIKFKAPETTELRPRITVLGIGGAGGNAVNNMIASKLEGVDFV
                     VANTDAQALAQSKAERRIQLGVHVTEGLGAGAQPDIGRAGAEESLDEILEQLTGSHMV
                     FITAGMGGGTGTGAAPVIARAVREQGILTVGVVTKPFSFEGDKRMRIAEKGVAELQQF
                     VHTLIVIPNQNLFRVANDRTTFAQAFAMADEVLHAGVRGVTDLIVMPGLINLDFADIK
                     SVISEMGKAMMGTGEAEGEDRAQKAADLAICNPLLDDVSMKGARGVLINITGGPDLML
                     FEVEQAANRVRAEVDPDANIIFGSTLIDNMEGRIRVSVVATGIDAAEMRMPKNVEPLR
                     PHIKPLRMPEPKPEPRPAMAAPVSSNPVHRQVEALADELGAAQTPVEAMILGGEDAPQ
                     VAVIGEDDIPPLPASIRPLREPVQEPVRPAPEPERRGWGLFGRNRKPKEEPRLEPVAR
                     PAASAPRATSQVMSRAAAPAEQLRAVQPPGPKPDDLFPEQKRDEQFDIPAFLRRQQN"
     gene            complement(4045..>5011)
                     /gene="ftsA"
                     /locus_tag="VGC27_02285"
     CDS             complement(4045..>5011)
                     /gene="ftsA"
                     /locus_tag="VGC27_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY0281433.1"
                     /translation="MALDGALVSDAHLRELLAEGRAKCAAEGYEIIQSAPTSYVVDEA
                     RGVRDPSGMFCQRIGVTMHAVAVKPSPLANLKLAVERCHLNVAGALFAPFASGLAVLS
                     DDEKQLGATVIDMGGGVTSIAVFLEGHLVHADVVPAGGSQVTADIARMLAAPLSAAER
                     NKVLYGAALGDLEVNADIVAMSQMGEEGEENDLRVPRSMLTRIIQARLEEIFTEVQNR
                     LRASGYDVAAGRRVVLTGGACQLAGVRELAARALNKQVRIGRPQSFVSGLPASAGGPD
                     YATAIGLLVAGATMSPDVLNPEAAPSQAETGSKGWLNRLTGGLLG"
BASE COUNT          850 a         1652 c         1610 g          899 t
ORIGIN      
        1 ctggcgcgcc cgccgccgcg tccgccggcg gccagaccca gcgcgtcgag caggatggat
       61 ttacccgccc cggtctcgcc ggtcagcacg ttgagaccgg cggcgaattc caggctcgcc
      121 tgctcgatca gaacgatgtc gcgtaccgtc agcgccgcga gcatcggcca gcctccagcg
      181 ctccctcact cttcgaccct tcgacaggct caggatgaag ggtgtgcgta tcggcaatcc
      241 cctcatgctg agcgtgccga agcatgagga ccgcgcctgt cagagaatct tgttgaacgc
      301 cctgctgatc catgagccct tgtcttcctc gggcttcaaa tcatgccctt tcagaagatt
      361 ataggcactt tcataccacg gactgccggg ataattcgcg cccagaacgg cggcggcggt
      421 ctgcgcctcc ttggtgatgc cgagcgcgta ataggcctcg gtcagccgct ccaaagcctc
      481 ggcgatctgg gaggttttct gatactgctc gaccacgacc cggaagcggt tgatggcgcc
      541 gatataatcg ccctggtaca gatagtagcg gccgacttcc atctccttgc cggccagatg
      601 gtccagcgtc aggtcgatct tcagcgtggc gtcgcgggcg tattcggaat ccggaaagcg
      661 ctggacgacg tcctgcaagg cgaccagggc ggcttccgtg ttcgactggt cgcggccgac
      721 atcgacgatc tgctcgtaga gcgaaaccgc cttcagataa aaggcgtagg agacctcttt
      781 gctgcccgga tgcagggaaa tgtactgatc cgcggccgtg atcgcatcgg agtacttgtt
      841 ggcctgataa tagcaaaagg cgctcatcaa actggcgcgg cgcgcccaca ccgaataggg
      901 atgctggcgc tcgacctcag cgaattgctg ggccgcctgg gaccaatccc cgccccggac
      961 tcgcctccag gcatcggaat agatctggtc gatcggacgt tcctggtagg tggcttcctt
     1021 cggcttgtcg gcatccgacc ctccgaaaag gctgcacccc gagagcgcca gcatgcttag
     1081 aacggcgcaa agaggaacaa atccgcgcgc aaaacccttc gagagcatca tcgactgttt
     1141 ccgtcttgag ggcaggacgc ttttttctct ggcgacctag tgtcccgatt ccgaaattcg
     1201 caccctctct atatcggctt cgggcgaatt tcggaatcga aaggacacta gcaacctatt
     1261 gaatcctagt gtcgttttgg tttcgaagtt cgcttacagc ccgatagtgc agatagagcg
     1321 aacttcgaaa ccacgacact agcctcttat gccacttggg cggaagcatg gtccagaggg
     1381 gtgcggcggc atcgtaaagg gaccgcccgg aacctctaag gccttgttac gaaaaagaaa
     1441 tcatccggta atttgccggg tctgcaaaca aggcgcgcag cagggcgttg ttgagggcat
     1501 ggccggagcg caccccttcg aagcgggcga tcaacggcgc ccccgccagg gccatgtcgc
     1561 cgatcgcgtc caaaatcttg tggcggacga attcgtcggg aaaccgcatc agcgaggcgt
     1621 tgacgatccg gtcgccgtcg atggccaggg tgttgtcgag cgaggcgccg tggccgcggt
     1681 cgatctgctt gagccgctcc aattcttgca ggaagccgaa ggtgcgcgcc ggcgcgatct
     1741 cgcggcggaa gccgtcaggc gagaacggga aggaaaagct ctgccggccg atggcggcgg
     1801 acgggaaggc aatctcgaaa tcgaacgaca aggcatcggc gggaacgagc gcggcgctgg
     1861 cgtccttatg caccgcttcc acccgcgtca gcaccttgat ggcgctgcgg ggcgcaggct
     1921 gcttcttgac gcccgccttg ccgatcaggt cgagatagga cagcgcgtcg ccatccagga
     1981 tcggcggctc cggcccgtcg agaatgacca gcagatcgtc gatcccggcc ccggcgacgg
     2041 ccgccatcag atgctcgacc acgcccacat tggcgcgcgc cgtcgccagc atggttccaa
     2101 gattggttcc ggagacggcg tcgtagcgcg ccgggatttc ggccccgccc aggtcagccc
     2161 ggcggaagac gatgccggtg ccgggcttgg ccggcgccag catcatgcgc acctgagtcc
     2221 cggcatgaag ggtgatgcct tcggcggcga cttcgcgggc gatggtgtgg cgcatcggca
     2281 tggtccgcgg agcataatgg ctttggactg ccgccgtata cccctggttc taaagacgaa
     2341 agggcggcgc ggaggtcatc cgcaccgccc ttcaatacct ggatcccctt cccctcgccc
     2401 tgctgacgca aggctcggcc ggggatgaca tccctggcgg gatgtcagtt ctgctgccgg
     2461 cgcaagaacg ccgggatgtc gaattgctcg tcgcgcttct gctccgggaa aagatcgtcg
     2521 ggcttgggtc ccggcggctg cacggcgcgc aactgctcgg ccggcgccgc cgcgcggctc
     2581 atgacctgcg aagtggcgcg cggggcgctc gccgcgggcc gtgcgaccgg ttccagacgc
     2641 ggctcttcct tgggcttgcg gttgcggccg aacaggcccc agccgcggcg ctccggttcg
     2701 ggcgcggggc gcaccggttc ttgcaccggt tcgcgcagcg gccggatgga ggccggaagc
     2761 ggcgggatgt cgtcttctcc gatcaccgcc acctgcggcg cgtcttcgcc gcccaggatc
     2821 atcgcctcga ccggcgtctg cgcggcgccc aactcgtcgg ccagcgcctc gacctggcga
     2881 tgtaccggat tggaactgac gggcgcggcc atcgccggac gaggttcggg cttcggttcc
     2941 ggcatgcgca gcggcttgat gtgcggccgc agcggctcga cgttcttggg catgcgcatt
     3001 tcggccgcgt cgatgccggt ggcgacgacg gaaacgcgga tgcgcccttc catattgtcg
     3061 atcagggtgc tgccgaaaat gatgttggcg tcgggatcga cctcggcgcg gacgcggttg
     3121 gcggcctgct cgacctcgaa cagcatcagg tccggcccgc cggtgatgtt gatgagcacg
     3181 ccgcgcgcgc ccttcatcga aacatcgtcg agcagcggat tgcagatcgc caggtcggcg
     3241 gccttctggg cacggtcttc gccctcggcc tcgccggtgc ccatcatcgc cttgcccatc
     3301 tccgagatca cgctcttgat gtcggcgaag tcgagattga tcaggccggg catcacgatc
     3361 aggtcggtga cgccgcgcac gccggcatgc agcacttcat cggccatagc gaaggcctgg
     3421 gcgaaggtgg tgcggtcgtt ggcgacgcgg aacaggttct ggttcgggat gacgatcagc
     3481 gtatgaacga actgctgaag ctcggcgacg cccttttcgg cgatgcgcat gcgcttgtcg
     3541 ccttcgaaag agaacggctt ggtcaccacg ccgacggtga ggatgccctg ctcgcgcacg
     3601 gcgcgcgcga tgacgggggc cgctccggtg ccggtgccgc cgcccatgcc cgcggtgatg
     3661 aacaccatgt gcgagccggt aagctgctcc agaatctcat ccagcgattc ctcggcgccg
     3721 gcgcggccga tatcgggctg cgcgcccgcg cccaagccct cggtgacatg cacgccgagc
     3781 tgaatgcgcc gttccgcctt ggattgggcc agcgcttgcg catcggtgtt cgccacgacg
     3841 aaatccacgc cttcgagctt ggacgcgatc atgttgttga ccgcgttacc gcccgcgccg
     3901 ccgatgccca gaaccgtgat ccttggacgc aactcggtcg tttcgggtgc tttaaacttg
     3961 atcgccataa tcattctcct tggctacgcg ctttttcact gccatctcat cacccaaacg
     4021 aatcaccctg acttcttggt tgaatcatcc gagcagccca ccggtcaacc ggttcagcca
     4081 ccctttcgag cctgtttccg cctgcgaagg cgctgcctcg ggattgagca cgtccggcga
     4141 catcgtcgcc ccggccacca agagcccgat cgccgtcgca taatcgggac ccccggcaga
     4201 cgccggcagg ccgctgacga agctctgcgg ccgcccaatc cgcacctgtt tgttgagcgc
     4261 gcgggcggcc agttcgcgca cgccggcgag ctggcaggcc ccgccggtga gcaccaccct
     4321 gcggccggcg gctacatcgt agcccgaggc gcgcaggcgg ttttgcactt cggtgaaaat
     4381 ctcttccagc cgcgcctgaa tgatgcgggt cagcatggag cgcggcaccc gcaagtcgtt
     4441 ttcctcgcct tcctcgccca tttgcgacat ggcgacgata tcggcgttga cctcgagatc
     4501 gcccagcgcg gcgccgtaaa gaaccttgtt gcgttccgcc gccgacagcg gcgccgccag
     4561 catgcgggcg atatcggccg tgacctggct gccgccggcc ggcaccacgt cggcatgaac
     4621 cagatggcct tcgaggaaga cggcgatcga ggtgacgccg ccgcccatgt cgatcaccgt
     4681 ggcgccgagc tgcttctcgt cgtcgctgag gaccgccagc ccgctggcga agggagcgaa
     4741 cagcgcgccc gcgacgttca ggtggcagcg ctccaccgcc agcttcaaat tcgccagcgg
     4801 cgaaggcttg accgcgaccg cgtgcatcgt caccccgatg cgctggcaga acatgcccga
     4861 agggtcgcgc acgccgcgcg cttcgtcgac cacatagctg gtgggggcgc tctgaatgat
     4921 ctcgtagccc tcggccgcgc atttggcccg cccttcggcg agcagttcgc gcagatgggc
     4981 gtccgacacc aaagcgccgt ccagcgccat g
//
LOCUS       DASWPU010000104         4725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_25165,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000104 DASWPU010000000
VERSION     DASWPU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25165"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..840)
                     /gene="flgH"
                     /locus_tag="VGC27_02290"
     CDS             complement(109..840)
                     /gene="flgH"
                     /locus_tag="VGC27_02290"
                     /inference="COORDINATES: protein motif:HMM:NF001305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY0281434.1"
                     /translation="MTLRTFKILLTLGAACLLAGCTTYGRLSELGSNPKLSPITNPAQ
                     KQPISVPMPQPPAEPRGANSLWQPGARSFFHDPRASRVGDILTVSISVADAAKISNTT
                     SRSRTNSEGASMANMFGLERYLPSSHMDPSSLVKMGSDTSNVGGGSVDRSEKINLTLA
                     ALVTQVLPNGNMVIAGHQQMRVNGELRDLQVSGIVRTEDITSANTVDLTQIAEARISY
                     GGQGQISRLQQPRYGQQVMDVLLPF"
     gene            complement(854..1339)
                     /gene="flgA"
                     /locus_tag="VGC27_02295"
     CDS             complement(854..1339)
                     /gene="flgA"
                     /locus_tag="VGC27_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY0281435.1"
                     /translation="MTRTILFACALLLCWPALADAGVRIVVPARDIARGTTISQADLT
                     FKTMDGNPLSGTVTSADDLVGMQTRRLLHAGESVRIEDVRRPTLVAKGSTVTMVFEAP
                     GITLTASGRAMSEGGLGETVTVQNPVSFRQVSAVVTGPGQVRAQNVGATLSSRLASAQ
                     P"
     gene            complement(1342..2130)
                     /gene="flgG"
                     /locus_tag="VGC27_02300"
     CDS             complement(1342..2130)
                     /gene="flgG"
                     /locus_tag="VGC27_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEY0281436.1"
                     /translation="MRALSIAATGMLAQQTNVEVIANNLANMNTTGFKQQRAEFEDLL
                     YQNVEQQGAQSSDTGTIVPSGIQLGAGVRTAAVYRVNRQGDLQSTSNPYDVAISGPGY
                     FRIQMPDGTDAYTRAGNFALSPEGQFVNQQGYIVQPGIAIPNTATDVAINAQGQVQAK
                     IPGQTEPQTVGQLELTRFPNEGGLKAEGNNLYTETVSSGAPQSGVPGSTGYGTIQHKF
                     LETSNVNAVAEITSLITAQRAYEMNSKVISAADQMMAQTAKLGG"
     gene            complement(2143..2883)
                     /gene="flgF"
                     /locus_tag="VGC27_02305"
     CDS             complement(2143..2883)
                     /gene="flgF"
                     /locus_tag="VGC27_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEY0281437.1"
                     /translation="MDNALLVSLSQQLAAYRSMDVIANNLANLSTPAFKRETVKFEEI
                     VQQVGPAGGRSGPQTLSFVQDRGVVRDLSEGRLERTGAPYDLAINGRGYFVVQTEAGE
                     RYTRNGHLSLNGDGQLITDSGDPIQGDGGPIVVTIDDGDISIAADGTITGKQGQMGKL
                     RVVDFADEHALQKEGESLYSTAQSPMAAKASSRIMQGTLETSNVEPVIEISRMIDVMR
                     AYQAMANLAQTQSDLKRQAIEKLGSTPS"
     gene            3043..3669
                     /locus_tag="VGC27_02310"
     CDS             3043..3669
                     /locus_tag="VGC27_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEY0281438.1"
                     /translation="MNHFLTTPEISSPQGLRAQKAMAKKENPAADVEEPEEGAEDGQK
                     AALKQDFVKKLLGNKKLLMIAGGGLLALLLGSGAGLYFFVSNGSGNSESAKLAAMPQF
                     PATPPRMVYHDVPDLIVNIQNADGSSAYLKLSVSLELYSEAEKPGLQALMPRIVDQFQ
                     GYLRELRVDDIKGSAGIMRLKEEMLRRINVAAAPYRVRDVLLKEMIVQ"
     gene            3685..>4725
                     /gene="fliM"
                     /locus_tag="VGC27_02315"
     CDS             3685..>4725
                     /gene="fliM"
                     /locus_tag="VGC27_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEY0281439.1"
                     /translation="MADDTENTEAAAEPPATEAPVAEAQPETGEPAPAGAAERILNQE
                     EIDSLLGFDVNSEQLQDKSGVRAIINSALVSYERLPMLEIVFDRLVRLMTTSLRNFTS
                     DNVEVSLDNITSIRFGDYLNSIPLPAILAVFHAEQLDNYGLFTVDSNLIYSIVDVLLG
                     GRRGSSAMRIEGRPYTTIERTLVQRMIEVILQDMSQAFEPLAPVNFQLDRLETNPRFA
                     AIARPANAAILVKLRVDMEDRGGRTELLLPYATLEPIRKLLLQQFMGEKFGRDSIWES
                     HLATELWSTRVEIEAILDEQIMPLNRMMNLEVGQTIMLNANPDSKVEMRCRGVPLLKG
                     RMGRVGASVAVRV"
BASE COUNT          844 a         1480 c         1504 g          897 t
ORIGIN      
        1 gcgtgccccc aaacaacgac ggccttccgt caggggagct gacggaaggc cgcgctggac
       61 acttcatggc gtgggagcgc tggatgaagt ggggagctgg agagagcgtt agaacggcag
      121 cagcacgtcc atcacctgct ggccgtagcg cggctgctga agccgcgaga tctggccctg
      181 gccgccatag gagatgcgcg cctcggcgat ctgggtcagg tcgaccgtgt tggcgctggt
      241 gatgtcttcg gtgcgcacga tgcccgagac ctgcaaatcg cgcagttcgc cgttgacgcg
      301 catctgctga tggccggcga tcaccatatt gccgttgggc agcacctgag tgaccaacgc
      361 ggccagcgtc agattgatct tctccgagcg gtcgacggaa ccgccgccga cgttcgaggt
      421 atccgatccc atcttcacca ggctcgacgg atccatgtgc gaggacggaa gatatctctc
      481 cagaccgaac atgttcgcca tgctggcgcc ctcggagttg gtgcgcgagc ggctggtcgt
      541 attggagatc ttcgcggcat cggccaccga gatgctgacc gtcaggatat cgccgacccg
      601 gctggcgcgc ggatcgtgga aaaagctgcg cgcgcccggc tgccacagcg agttcgcgcc
      661 gcgcggctcg gccggcggct gcggcatcgg caccgaaatc ggctgcttct gcgccggatt
      721 ggtaatgggg gaaagtttgg ggttgctgcc gagttccgac agccgcccgt aggtggtgca
      781 acccgcaagc aggcaggccg cgcccagcgt gagaagaatt ttgaaagtgc gcaaggtcat
      841 ggttcggatt cctttacggc tgagcgctgg caaggcgcga agacagggtt gcgccgacat
      901 tctgggcgcg cacctgaccg gggcctgtta ccacggcgct gacttggcgg aacgaaaccg
      961 ggttctgcac cgtcacggtt tctcccagtc cgccttcgct catggcgcgc ccggaagcgg
     1021 tgagcgtgat gcccggcgct tcgaaaacca tcgtgaccgt cgagcctttc gccaccagcg
     1081 tcgggcggcg cacatcttcg atgcgcacac tttcgccggc atgcagcaag cggcgcgtct
     1141 gcattccgac cagatcgtcc gccgaagtca cggttcccga caatgggttg ccgtccatcg
     1201 tcttgaaggt cagatcggcc tgggagatcg tcgtgccgcg cgcgatatcg cgggcgggca
     1261 ccacgatgcg gaccccggca tcggcaagag ccggccagca cagcagcagg gcgcaagcga
     1321 aaagaatggt gcgcgtcatg gtcagcctcc cagcttcgcg gtttgcgcca tcatctggtc
     1381 ggcggcgctg ataaccttcg aattcatttc ataggcgcgt tgcgcggtga tcagcgaggt
     1441 gatttccgcc accgcgttga cgttcgatgt ctccagaaac ttgtgttgga tcgtgccgta
     1501 accggtggag ccgggaacgc ccgactgcgg cgcccccgac gagacggttt cggtatacaa
     1561 attgttgccc tcggccttga ggcccccctc gttggggaag cgcgtcagtt ccaactggcc
     1621 gacggtctgc ggctccgtct ggccgggtat cttagcctgc acctggccct gcgcattgat
     1681 cgcaacatcc gtggcggtat tcggaatggc gatgccgggc tgcacgatat agccctgctg
     1741 gttgacgaac tggccttccg gcgacagcgc gaaattgccg gcgcgggtat aggcgtcggt
     1801 gccgtcgggc atctggatgc ggaaataacc cggcccgctg atcgcgacat cgtagggatt
     1861 cgaggtcgac tgcagatcgc cctgccggtt gacgcgatag acggccgcgg tgcgcacgcc
     1921 ggcgccgagc tggatgcccg acggcacgat ggtgccggta tcggaagact gggcgccttg
     1981 ctgctcgacg ttctgataca gcagatcctc gaattcggcg cgctgctgct tgaatccggt
     2041 ggtgttcatg ttcgccagat tgttggcgat gacttcgaca ttggtctgct gggcgagcat
     2101 tccggtcgct gcgatgctga gggcgcgcat gggtaattct ccttaactgg gggtgctgcc
     2161 gagcttctcg atggcttgac gtttcaaatc gctttgggtc tgcgccagat tggccatcgc
     2221 ctgataggcg cgcatgacgt cgatcatgcg ggaaatctcg atcaccggct cgacgttcga
     2281 ggtttccagc gtcccttgca tgatcctgga gctcgccttt gccgccatcg ggctttgagc
     2341 ggtggaataa aggctctcgc cttccttctg caacgcgtgc tcgtcggcga aatccaccac
     2401 cctgagcttg cccatctggc cttgcttgcc ggtgatcgtg ccgtcggcgg cgatggaaat
     2461 gtcgccgtcg tcgatcgtga cgacgatcgg gccgccgtcg ccctggatcg gatcgccgct
     2521 gtcggtgatc aactgcccgt cgccgttgag gctgagatgg ccgttgcggg tgtagcgttc
     2581 gccggcctcg gtctgcacca cgaaatagcc ccggccgttg atggcgaggt cgtaaggcgc
     2641 gccggttctt tccagccgtc cttcgctcag atcgcgcacc acacccctat cctggacgaa
     2701 actcagcgtc tgcgggccgc tccggccacc ggccggcccg acctgctgaa cgatctcctc
     2761 gaatttcacc gtctcgcgct tgaaggccgg cgtcgacagg ttcgcgagat tgttcgcgat
     2821 cacgtccatc gagcgatagg ctgcgagttg ttgtgagaga ctgacgagca acgcattatc
     2881 catggggcac ggatcctttc tggtccttag ccatagccac ggggttgcag gctgcatgcc
     2941 attcgcaacc gcccggtttc cacggatttt caagaccgcc gaacgcggca ccggcaatat
     3001 ctgccgggtg aagtctgccc tggcctgcaa ttgagggggg acttgaacca cttcttaacc
     3061 acgccggaaa tatcgtcgcc tcagggattg cgggcgcaga aggcgatggc gaagaaggaa
     3121 aacccggcgg ctgacgtcga ggagcccgaa gagggcgccg aggacgggca gaaagcggct
     3181 ctgaagcaag actttgttaa gaaacttctt ggcaacaaga agcttctgat gattgccggc
     3241 ggcggcttgc tggccttgct gctcgggagc ggcgcgggac tttacttctt tgtttccaac
     3301 ggctccggca atagcgagag tgccaagctc gcggcgatgc cgcagtttcc ggccacgccg
     3361 ccccggatgg tctatcacga cgtccccgac ctgatcgtga acatccagaa cgcggacggt
     3421 tcgtcggctt atctcaagct gtcggtgtcg ctcgaactct attccgaggc cgaaaagcca
     3481 ggtttgcagg cgctgatgcc gcgcatcgtc gatcagttcc agggttatct gcgcgaactg
     3541 cgcgtggacg acatcaaggg ctcggccggc atcatgcgcc tgaaggaaga aatgctccgc
     3601 cggatcaacg tcgccgccgc gccatatcgc gtccgcgacg tcctcctcaa ggaaatgatc
     3661 gttcagtagg accacagaaa aaagatggcc gacgacaccg agaacaccga agccgccgca
     3721 gaaccgcctg ccaccgaagc gcccgtcgcg gaggcgcagc cggaaacggg cgaacctgcg
     3781 ccggcgggcg ccgccgaacg catcctcaac caggaggaga tcgactccct cctcggtttc
     3841 gacgtcaaca gcgaacagtt gcaggacaag tccggcgtcc gcgccatcat caactcggcg
     3901 ctggtctcct acgagcgcct gccgatgctg gaaatcgtct tcgaccggct ggtccggctg
     3961 atgacgacga gccttcgcaa tttcacctcg gacaatgtgg aggtgagcct ggacaacatc
     4021 acctccatcc gcttcgggga ttacctgaat tccatccccc tgcccgcgat cctggccgtg
     4081 ttccacgccg agcagttgga caattacggc ctgttcacgg tggattccaa cctcatctac
     4141 tccatcgtcg acgtgctgct gggcggacgg cgcggctcct cggccatgcg catcgagggc
     4201 cggccttaca ccaccatcga acgcaccctc gtccagcgca tgatcgaagt catcctgcag
     4261 gatatgtccc aggccttcga acctctggcg ccggtcaatt tccagctcga ccgcctggaa
     4321 accaatccgc gtttcgcggc gatcgcgcgg ccggccaacg cggcgatcct ggtcaagctg
     4381 cgcgtcgaca tggaagaccg cggcgggcgc accgagctgc tgctgcccta cgccaccctc
     4441 gaaccgatcc gcaaactcct gctgcagcag ttcatgggcg agaaattcgg ccgcgactcg
     4501 atctgggaaa gccatctggc gaccgagctg tggtccacgc gcgtcgagat cgaggcgatc
     4561 ctggacgagc agatcatgcc gctgaaccgc atgatgaacc tggaagtcgg ccagaccatc
     4621 atgctgaacg ccaatccgga ttcgaaagtc gagatgcgct gccgcggcgt gcccctgctg
     4681 aaggggcgca tgggccgcgt cggggcatcg gttgccgtgc gcgtc
//
LOCUS       DASWPU010000105         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_43756,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000105 DASWPU010000000
VERSION     DASWPU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43756"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VGC27_02320"
     CDS             complement(<1..662)
                     /locus_tag="VGC27_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0281440.1"
                     /translation="MPIDQSDIAFANKLADAAGEAILPYFRQRIEVTDKGEGFFDPVT
                     AADKGAEAAIRALIKAERPDDGILGEEYGEEAGTSARRWVLDPVDGTRAFIAGQPMWG
                     TLIALEEAGKPVLGVLDQPFLKERYIGHDGTASLSDRTGARPLAVRRCAALPEAVICT
                     THPFAHLDDDDRARFRRVELQARLSRYGGDCYAYALVAMGFIDLVIETNLKRWDIAAI
                     VP"
     gene            873..1172
                     /locus_tag="VGC27_02325"
     CDS             873..1172
                     /locus_tag="VGC27_02325"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY0281441.1"
                     /translation="MARQDRIRVEWDPRKSDQNRRKHGIDFGFAMLVFYDPLKSIEIE
                     GHEHGETRWRTVGEIDGRVFVVSHTVREEDDIEIIRIISARKASRRERQKYEEAP"
     gene            1141..1458
                     /locus_tag="VGC27_02330"
     CDS             1141..1458
                     /locus_tag="VGC27_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0281442.1"
                     /translation="MNVKNMRKRPKLTADQRRQLEALDKLPDDRIDFSDIPETTEEDW
                     SRPHYVGLMYRPGKKSVTIRLDEDMVKWFKAQGKGWQTKMNWALRLYFASHRKRGAVV
                     GKP"
     gene            complement(1455..>1793)
                     /locus_tag="VGC27_02335"
     CDS             complement(1455..>1793)
                     /locus_tag="VGC27_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY0281443.1"
                     /translation="EAVPYALWYLEERGKLFVVPGTKVYCGMIIGQHAKPNDLDVNPL
                     RAKQLTNIRTTSKDEAVRLTPIVPMTLEQAIAYIEDDELVEVTPQSIRIRKKNLSAHE
                     RKRAARADAD"
BASE COUNT          370 a          534 c          552 g          337 t
ORIGIN      
        1 atgggaacga tcgcggcgat atcccagcgc ttcaggttcg tctcgatcac cagatcgatg
       61 aaacccatcg ccaccagcgc ataggcgtag caatccccgc cgtagcgcga cagccgggcc
      121 tgcaattcga cgcgccggaa ccgcgcccgg tcgtcgtcgt ccagatgcgc gaagggatgg
      181 gtcgtgcaga tcaccgcttc cggcaaggcg gcgcagcggc gcaccgcaag cgggcgcgcg
      241 ccggtccggt cgctgagcga cgccgtgccg tcatggccga tatagcgttc cttcaggaag
      301 ggctggtcga gcacgcccag cacgggctta ccggcttctt ccagcgcgat cagcgtgccc
      361 cacatcggct ggcccgcgat gaaggcgcgc gtgccgtcca ccggatcgag cacccagcgc
      421 cgggcgctgg tgcccgcctc ttccccgtat tcctcgccca agatgccgtc gtccggccgc
      481 tccgccttga tgagcgcgcg aatggccgcc tccgcgccct tgtcggcggc ggtgaccgga
      541 tcgaagaagc cttcgccctt gtcggtcacc tcgatgcgct gacggaaata gggaaggatc
      601 gcctcccccg ccgcatcggc aagcttgttg gcgaaggcaa tgtcggattg gtcgatgggc
      661 atggcccggc tcctcttgcg ccactcctat attgtatgcg gaccaagaga gaaatagtgc
      721 atgataaagc gtcgttgcca gggcagcgtc caccagcatc ggcagctttt gcaggattga
      781 catcggcgcc atttgtacgt acattttgta cgtacattag tgacaacaat actctcggcg
      841 acaaggtcaa ttcatgacac agagattgtg caatggcgag acaagatcgc atccgcgtcg
      901 agtgggatcc acgcaagtcg gatcaaaacc ggcgcaagca cggaatcgac ttcggcttcg
      961 ccatgctggt cttttacgac ccgctgaaga gcatcgagat cgagggccat gaacatggag
     1021 aaacccgctg gcggacggtc ggtgaaatcg atggaagagt tttcgtcgtc tcccacacgg
     1081 tccgtgaaga agacgacatc gaaatcatcc gcatcatctc ggcgcgaaaa gcttcacgtc
     1141 gtgaacgtca aaaatatgag gaagcgccct aagctgacag ccgatcaacg gcgacaactg
     1201 gaagcgttgg ataaattgcc cgacgatcgg atcgacttca gtgatattcc ggaaacgact
     1261 gaagaggact ggagccgccc gcattacgtc ggactgatgt accgaccggg aaagaaaagc
     1321 gtcaccatcc gactcgacga agacatggtg aagtggttca aagcgcaagg aaaaggttgg
     1381 cagaccaaga tgaattgggc attgagactg tattttgcga gccaccgcaa gaggggtgcg
     1441 gttgtcggca aaccttagtc cgcgtctgcc cgcgccgcgc gtttgcgctc atgggcgctg
     1501 aggtttttct tgcggatgcg gatcgactgg ggagtgacct ccaccagctc gtcgtcctcg
     1561 atataggcga tcgcctgttc cagcgtcatc ggcacgatcg gcgtcaggcg caccgcctcg
     1621 tccttgctgg tggtgcggat gttggtgagc tgcttggcgc gcagcggatt gacgtcgaga
     1681 tcgttgggct tggcgtgctg gccgatgatc atgccgcaat agaccttggt gcccggcacg
     1741 acgaacagct tgccgcgctc ttccagatac cacagcgcgt agggcaccgc ttc
//
LOCUS       DASWPU010000106         3475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_6934, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000106 DASWPU010000000
VERSION     DASWPU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6934"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VGC27_02340"
     CDS             <1..487
                     /locus_tag="VGC27_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281444.1"
                     /translation="GGERPKYAPGVMAAIYRSFVGQSRLFNRIDGLIGGIQFKMYGGY
                     DRPLSAEATKTYERDSIALTRMLLTKLREKYRDAPAVMVNCDGNESGPNKQWKALARD
                     AGFIPLSGPSDFLLSVKPGDRRDIFHADGAHLSEEGNRLYGTIAGDEIVSLRLAALNR
                     R"
     gene            complement(504..1631)
                     /locus_tag="VGC27_02345"
     CDS             complement(504..1631)
                     /locus_tag="VGC27_02345"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY0281445.1"
                     /translation="MRVRKLAGLAAYVLFVAVFVEISLQLFYYAMAGDFLFNRSAPPL
                     YQSEPYAGYMNRPGLSYDHHTSEYEARYYTNQAGFRVAKPGLEYSVRKPANTYRIVLL
                     GPSFAYGWGVDYDKSFAALLPRLLAERGFAQGRKIELIDAGVPSLEPAQQLNWYEHVG
                     KKYQPDLVIQFIYGSMAVKSNPRSSMAADAKGYLIRSDLSASQRWRERAKHFATVFYG
                     WMLWTDFDAWRNARPAAPNGAVMGAGRELVQQTKFDLDKPGIRDATLFYDRLAKTVRE
                     SGARLQIVYFPLSYVIYPDDVGRWRHLGVRNVEAQEAFDAAFVSYLNGRHIPAVDITD
                     DLRKAAVHEKRLYYWLDIHWTPEGNLAAARAVADTLLRTAG"
     gene            1843..2268
                     /locus_tag="VGC27_02350"
     CDS             1843..2268
                     /locus_tag="VGC27_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281446.1"
                     /translation="MATNFHEHFEERIELPSDRSTGLVFALVGVVFAVIYHRNAVILS
                     VGLSLAVILGGVSFFAPILLRPLNIVWFKLGLLLGKIVGPVVMGVLFFLVIVPFGYVM
                     RLRYDPLRAKIKPNPESYWIERDCSPSGEGMTASMKNQF"
     gene            2299..2451
                     /locus_tag="VGC27_02355"
     CDS             2299..2451
                     /locus_tag="VGC27_02355"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEY0281447.1"
                     /translation="MRTFAKEFLAYLGARKKMWLLPIILTLMLIGGLLVLAQGSAVAP
                     FIYTIF"
     gene            2478..3475
                     /locus_tag="VGC27_02360"
                     /pseudo
     CDS             2478..3475
                     /locus_tag="VGC27_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
BASE COUNT          693 a          996 c         1050 g          736 t
ORIGIN      
        1 gggcggcgag cgccccaaat acgcgccagg agtcatggct gcaatctatc gctcgttcgt
       61 cggccaatcg cggctcttca acaggatcga tggtctgata ggcggcatac agttcaaaat
      121 gtacggcgga tacgacaggc cgctttccgc cgaagcgacg aagacgtatg agcgcgactc
      181 tattgcctta acgagaatgc tgctcacgaa gctcagagag aagtaccgcg atgctccggc
      241 cgtcatggtg aactgcgacg gaaatgaatc cggtccgaac aagcaatgga aggcccttgc
      301 tcgcgacgcc gggttcattc ctctcagcgg cccgtccgac tttctgcttt ctgtgaagcc
      361 tggtgatcga agagacatct tccatgcgga cggggcgcat ctttcggaag aaggcaaccg
      421 gttgtacgga accatcgcgg gcgacgagat agtgtctctg aggttggccg cgctgaaccg
      481 gcggtaaaag cgagaccgga aaattatccg gcggtgcgca gcaaggtgtc ggcgacggcg
      541 cgcgcggcgg ccaaattccc ctccggggtc cagtgaatgt cgagccaata gtagagccgc
      601 ttttcgtgga cagcggcctt gcgaagatcg tccgtaatat cgaccgccgg aatatgccgc
      661 ccgttgaggt agcttacgaa agccgcgtcg aaggcttctt gcgcttcgac gttgcgcaca
      721 cccaggtgac gccagcggcc cacgtcgtcg gggtagataa cgtagctcag cgggaaatac
      781 acgatttgca gccgcgcgcc ggactcgcgc acggtcttcg caaggcggtc gtagaagagc
      841 gtcgcatcgc gtatccccgg tttatcgagg tcgaacttgg tctgctggac cagttcgcgg
      901 ccggccccca tcacggcgcc gttgggtgcg gcgggcctcg cgttccgcca agcgtcgaaa
      961 tcggtccaca gcatccagcc gtaaaagacc gttgcgaaat gcttggcgcg ctcccgccag
     1021 cgctgacttg ccgacaggtc gctccggatc aaatatccct tggcgtccgc cgccatgctc
     1081 gatcgcgggt tgctcttcac ggccatcgaa ccgtagatga attgaatgac gagatcgggc
     1141 tgatatttct tgccgacatg ctcataccag ttgagctgct gcgccggctc caacgagggt
     1201 actccggcgt cgatgagttc gatcttgcgg ccctgggcga agccccgctc cgccaggagc
     1261 cgcggaagca aggccgcgaa gctcttgtcg taatcgacgc cccagccgta ggcgaaggaa
     1321 gggccgagca gaacgatccg ataggtgttg gcgggctttc tgacggaata ttcgaggccc
     1381 ggtttcgcga cgcggaaccc tgcctgattg gtatagtaac gcgcctcgta ttcgcttgtg
     1441 tggtggtcgt acgaaagccc gggacggttc atgtaaccgg cataaggctc gctctggtac
     1501 aggggcggag cgctccggtt gaacagaaaa tcgccggcca tggcgtagta gaaaagctga
     1561 agcgaaatct caacgaaaac ggcgacgaaa agaacatacg ccgcgagccc cgcgagcttt
     1621 cgcacccgca tgctgcttcc gattcttcgg aggagagctt ccatgcaaag agatttacag
     1681 gcccgttcgc tcgtttgtcc agttgcctcg ccaagccgcc caaagccggg aggagacccc
     1741 gtctcgagcc ggtgacggca cgccttccgg ttcgcgaggc cgccgctttc agccaaaatc
     1801 ccctatcgtt tcgaagcgtt aaggtgctat cgtcggagag atatggcgac caattttcac
     1861 gaacatttcg aggaacggat cgagcttcct tccgaccgct cgaccgggct tgtctttgcg
     1921 ctggttggcg tcgtgtttgc ggttatctat catcggaacg cggtgatttt atccgtcggc
     1981 ttgtcccttg cggtcatctt gggcggcgta agcttttttg ctcctatcct gctgcggcct
     2041 ctgaacatcg tctggttcaa gctcgggctg cttttgggca agattgtagg tcccgtggta
     2101 atgggggtgc tgttctttct ggtcatcgtt cccttcggtt atgtcatgcg gttgcgttat
     2161 gacccgttgc gggcgaagat taagcccaat cccgagagtt attggatcga gcgagattgc
     2221 agcccatccg gggaaggcat gacggcgtcc atgaagaacc aattctagtt ccgaatgtat
     2281 tgaagggggt gcatctcgat gaggactttc gccaaggagt ttcttgccta tctcggggcg
     2341 cgcaagaaga tgtggctgct gcccattatc ctcacgctca tgttgattgg gggactgctt
     2401 gtcctcgccc agggttccgc cgtcgcgccg ttcatctata cgatcttcta gcgcggcgac
     2461 gcttgggtct ttgagcaatg cgaattctgg gaatttccgc gttctaccac gacagcgccg
     2521 ccgcgctcgt cgaagacggg gaaatcgtcg ccgccgcgca ggaagagcgt ttcacccgga
     2581 agaaattcga cgcacgcttc ccgaagagtg cgatcggcta atgtctggcc gaagccggcg
     2641 tcagcttgga cgatgtcgac catattgtct ttttcgagaa gccgttcatc aaattcgaac
     2701 gcctgctgga aacctatctg gcaaacgcgc caaggggatt tgcctccttc cgcagagcga
     2761 tgccgctctg gatcaaggaa aaattattcc agaagtcgat gctgctgaag gagctggcgc
     2821 cgttcgcatc ctccggcggt cttgaagaga gattgctctt cgccgatcat caccaaagcc
     2881 acgccgcggc ggcgtttttc ccgtcgccgt tcgatgaagc ggtgatcttg acgctggacg
     2941 gcgtgggcga atggaccacg gcctcggtgg cgatcggcag aggcggcacc atcgaaaccg
     3001 tgcgcgaaat caagtggccg cattcgctcg gcttgttgta ttcggctttc acctattaca
     3061 cgggcttcag ggtcaactcg ggcgaatata aggtcatggg ccttgcccct tacggcagcc
     3121 cgaaatatgc cgacctcatc ttcaagcata tcgtcaacgt cgccgaggac ggcagcttct
     3181 ggctggatca gagctatttc gattacgcca ccggattgag gatgacgaac aagaagttcg
     3241 accggctgtt cgacggcccg ccgcggcgcc ccgaaagccc cttgacgcag cgcgacatgg
     3301 accttgccgc gtccgttcag gcggtgacgg aagaaatcct gctgcgcatc acccgggaac
     3361 tcgcccgcaa atacgcgctc gataatctgt gccttgccgg aggggtggcg ctgaattgcg
     3421 tcgccaacgg aaagattttg cgggatggaa aattcaagcg catctggatt caacc
//
LOCUS       DASWPU010000107         3023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_13030,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000107 DASWPU010000000
VERSION     DASWPU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13030"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1093)
                     /locus_tag="VGC27_02365"
     CDS             complement(59..1093)
                     /locus_tag="VGC27_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY0281448.1"
                     /translation="MKTFYSAVHFGHDPLTQFEGGVLAPAVEIPIRAERVKAEVEERK
                     IGPVLAPAQFDDAPILRVHDAGLVNFLGVAHAEWRQRYGENAPAALPSAWPAHGLRDG
                     RNGDIEAKLGSYAFSADTPILRGTWAAAREAVNVALSAAQAIRAGDKSAFALTRPPGH
                     HAAFDVYGGFCYLNNIAIAAQWLADAGMRPAILDVDYHHGNGTQAIFYGRPDVFVCSL
                     HADPHFAYPHYLGFVDEQGEGAGAGYNLNLPLPADTEWGVYEQALTLGLKAVEAFGPD
                     VLLVSLGLDTYINDPIASFRLKQADYLRLGEKLAALKHPTLFVFEGGYDIEALGEITA
                     NVLEGFESAI"
     gene            1342..1995
                     /locus_tag="VGC27_02370"
     CDS             1342..1995
                     /locus_tag="VGC27_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281449.1"
                     /translation="MKRVAKLTFAALLLAGTAALTATPASARVGIGLSFGYGPGFVPF
                     SDPCDYYNYYNEPPPWGLPPDYCNYPVYFDPVYYGGSWYRGPIYYRWYGGQRLYWLNG
                     GWRHDGWRGARPSIHWQDRGGWGHRSGWNRGGYNRSHSYNSGGSRWSGSGHNWQDRGR
                     SNWSGDSNRGRSGSGHTSGYSSGRSSGGGHSSGYSGGRSGGGHSSGGGHGGGGGHHR"
     gene            2134..2829
                     /locus_tag="VGC27_02375"
     CDS             2134..2829
                     /locus_tag="VGC27_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281450.1"
                     /translation="MRTFLKTTVAAAAIAGAALFSAAPANAQDISFSFGSGGVGFSYD
                     SGGYCDSWGCPDDFWDYPIYYCPVYFRGSWYNGPLYFRQYGGEYWYWIQGNWRRDQWR
                     SMRPNWACLDRYGPPLGFEYYENNGFRIRSEWRDRWRRNYNRDHGNWNGREHRYNWQG
                     GGSDWHRLWQRQHDNDRGGRGRGGNDRGGQDRGGQGGGQRDGNQGGPGGQFGHRGVGQ
                     SGGAQGGGQRDGN"
     gene            2816..>3023
                     /locus_tag="VGC27_02380"
     CDS             2816..>3023
                     /locus_tag="VGC27_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281451.1"
                     /translation="MTAIKAAQADSPATGASVKAAVPKAAASVTATKAAGKATRVTAV
                     APFQPSVQTARSTRLRPRRPATRAA"
BASE COUNT          541 a          935 c         1026 g          521 t
ORIGIN      
        1 cgcccgccgg agctttagcg taggcgggtc gaggggtgag gcgaccgctt cggccggttc
       61 aaatcgcgct ctcgaatcct tccagcacat tcgcggtgat ttcgcccagc gcctcgatgt
      121 cgtagccgcc ttcgaacacg aacagggtcg ggtgcttcag cgccgcgagt ttttcgccca
      181 ggcgcagata gtcggcttgc ttcagccgga acgaggcgat gggatcgttg atatacgtgt
      241 cgaggccgag cgaaaccagc agcacgtcgg gcccgaacgc ttccaccgcc ttgaggccga
      301 gagtcaacgc ctgctcgtaa actccccatt ccgtatctgc gggcagcggc agattgagat
      361 tgtagcccgc gcccgcgcct tcgccctgtt cgtccacgaa gcccaggtaa tgcggatagg
      421 cgaagtgcgg atcggcgtgc agcgagcaga cgaacacgtc ggggcggccg tagaagatcg
      481 cttgcgtgcc gttgccgtgg tggtaatcga cgtcgagaat cgcgggccgc attcccgcgt
      541 cggccagcca ttgcgcggcg atggcgatgt tgttgagata gcagaagccg ccgtacacgt
      601 cgaaagcggc gtgatggccc ggcgggcggg tcagcgcgaa ggcgctcttg tcgccggcgc
      661 ggatcgcctg cgccgccgag agcgcgacat tcaccgcctc gcgcgccgcc gcccaggtgc
      721 cgcgcaggat cggcgtgtcg gcgctgaagg cgtagctgcc gagcttggct tcgatgtcgc
      781 cgttgcgccc gtcgcgcagg ccgtgcgccg gccaggccga gggcagggcg gcgggagcgt
      841 tttcgccgta acgctggcgc cattcggcgt gcgccacgcc gaggaaattc accagccccg
      901 cgtcatggac gcgcaggatc ggcgcatcgt cgaattgcgc cggcgccaaa accgggccga
      961 tctttcgctc ttccacttcg gccttgacgc gctcggcgcg aatgggaatt tccacggcgg
     1021 gcgccagcac gccgccctcg aactgcgtca acgggtcgtg gccaaaatgg acggcggaat
     1081 agaaggtttt catccctgcg tttgtagcat aatcgcttgg cacgccgtag ctccggcgaa
     1141 agcggccgcg cgggttttcg acggctggcc gccgcgaagc gcttgccgga ttatggccga
     1201 ttgaaggcaa cggcggtttg gcgcggtttt ccgtcgcttt cggagggcgg atttcttcat
     1261 cctgacaata tcgttcatgc gcggttcagc cgttgcgtct cagattcatg tcaacgaatt
     1321 ggcgaacaag gagacaaaac catgaaacga gtcgccaaac ttacattcgc ggcattgctg
     1381 ttggccggca ccgccgcgct gacggcaacg ccggcctccg cgagagtcgg catcggcctc
     1441 tcgttcggtt acggtccggg cttcgtgccg ttcagcgatc cgtgcgacta ttacaactat
     1501 tacaacgaac cgccgccttg gggtctgccg ccggattact gcaactatcc ggtctatttc
     1561 gatcccgtct attacggcgg cagctggtat cgcgggccga tctattaccg ctggtacggc
     1621 ggccagcgtc tttactggct caacggcggc tggcgccacg acggatggcg cggcgcgcgg
     1681 cccagcatcc actggcagga tcgcggcggc tggggacata gaagcggctg gaaccgtggc
     1741 gggtacaacc gcagccactc ctacaattcc ggcggcagcc gttggtcggg cagcggacat
     1801 aattggcaag accggggccg cagcaattgg tccggcgaca gcaaccgcgg cagatccggt
     1861 agcggacaca ccagcggcta tagcagtgga cgcagcagcg gcggcggaca cagcagcggc
     1921 tatagcggtg gacgcagcgg cggcggacac agcagcggtg gcggccatgg cggcggcggc
     1981 ggacatcacc gctagctgaa cttctctgca ccacgcctgg gcggttccgg catatgttca
     2041 ccggaatcgc ctcgcgggtg atctgcggca aggcaatgac aagctgcgca cacgggaata
     2101 gacggctaag ctgataaagg gagctggctt gcgatgagga ctttcttgaa gaccacggtc
     2161 gccgctgcgg cgattgccgg tgcggcgctg ttctcggcgg cacccgccaa cgcccaggat
     2221 atttctttca gttttggctc gggcggagtc ggtttttcct atgacagcgg gggatactgc
     2281 gactcctggg gctgtcccga tgatttctgg gattatccga tttattattg cccggtgtat
     2341 ttccgcggca gctggtacaa cgggcccctc tacttccgcc aatatggcgg ggagtattgg
     2401 tactggatcc agggcaattg gcggcgcgat cagtggcgca gcatgcgacc gaactgggct
     2461 tgtctagacc gctatggtcc gccgctgggc ttcgaatatt acgagaacaa cggcttcagg
     2521 atacgcagcg aatggcgcga ccgttggcgc cgcaattaca accgcgatca tggcaattgg
     2581 aacggtcgcg aacatcgcta caactggcag ggtggcggca gcgattggca tcgcttgtgg
     2641 cagcgccagc acgacaatga tcgcggcggt agaggtcgcg gcggcaacga ccgcggcgga
     2701 caagaccgcg gcggccaagg cggcggtcag cgcgacggca atcaaggcgg cccaggcgga
     2761 cagttcggcc atcggggcgt cggtcaaagc ggcggtgccc aaggcggcgg ccagcgtgac
     2821 ggcaattaag gcggcccagg cggacagtcc ggccaccggg gcgtcggtca aagcggcggt
     2881 gcccaaggcg gcggccagcg tgacggcaac caaagcggcc ggcaaggcaa ccagagtgac
     2941 ggccgtcgct ccattccagc cttcagtcca aaccgcccgg tcaacacgcc tccgcccgcg
     3001 ccggccggca acacgggcgg cca
//
LOCUS       DASWPU010000108         3032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_110938,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000108 DASWPU010000000
VERSION     DASWPU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110938"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VGC27_02385"
     CDS             complement(<1..1134)
                     /locus_tag="VGC27_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0281452.1"
                     /translation="MAHAAAVPSIESLAGMDAKLEALAKFEALAEETETKGARGEANG
                     RLCRAIEAFRRGDAAQAAKWALAATTADPSCAQAFHVLAMALEKMGHVREALATYERA
                     FQLDPDDPDLLLDLGLTAWNQNVKAGAEQMFRLYIAARPDSPLGYNNLGSIQSDMGDS
                     TMAIETLRSAIMRLPNEAILWNSLATVLAEDGRVEESLVFYREAIRLDPAFARFHHNL
                     GYALAHLGRLAEALEAYDNAMALVAEPDERIEALHSRAVCLIGMGRLEEGFREYEIRN
                     NERFRAYVHHMIKAPLWKGESLEGRRILVTGEQGLGDEIMFADVLPDIQRAVGPKGKL
                     QIAVDPRLVPLFQRSFPEAEVGTYDDRKLIDKSGNKELRFVPFA"
     gene            1288..1716
                     /gene="flbT"
                     /locus_tag="VGC27_02390"
     CDS             1288..1716
                     /gene="flbT"
                     /locus_tag="VGC27_02390"
                     /inference="COORDINATES: protein motif:HMM:NF001995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048027 - mRNA 5'-UTR binding [Evidence
                     IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HEY0281453.1"
                     /translation="MPLKLSLKPGEKFVLNGAVLTNGDKKKTSLVIQNKACILREKDI
                     MQPEEATTPARHIYLPIMMMYLDPEGIETYYNNFALRMTEFMGAIQNRSALAVCIEIS
                     RDVMSGDYYKALMLCRKLFEFEQERLNHDPESLSKRVAYS"
     gene            1676..2026
                     /gene="flaF"
                     /locus_tag="VGC27_02395"
     CDS             1676..2026
                     /gene="flaF"
                     /locus_tag="VGC27_02395"
                     /inference="COORDINATES: protein motif:HMM:NF009435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HEY0281454.1"
                     /translation="MTLKAYQNALRIAEEPRQTEYRLFGQVTGALINAQKVHASGGPL
                     VETIDWNRRVWRTLAADCLDETNQLPKDVRAQIVSLSLWVTKYSKEVTRQGAPLDPLI
                     TINRTIMEGLRGAA"
     gene            2281..>3032
                     /locus_tag="VGC27_02400"
     CDS             2281..>3032
                     /locus_tag="VGC27_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY0281455.1"
                     /translation="MLSVNTNPGALTALQYLNSTNAQLQTAENAINSGLKVASAKDNG
                     AIFAIAQNMRGNVAGYSAVSDSLNRASSVVDTGLSAAQSVSDLLIQMKQNALAAADTS
                     LDATSRASLARDFQALSDQITTIVENASFNGINIVNGAGTGVSALASANDPDQTISVA
                     AQDLTVTNLDVDAAYTNQASAKAMVGTVSTAIATVNTILSTLSSGSKKFSIQASFVSK
                     LSDTLTSGIGNLVDADMAKESALLTSLQTKQQL"
BASE COUNT          624 a          914 c          917 g          577 t
ORIGIN      
        1 cgcgaaggga acgaagcgaa gctccttgtt gccgctcttg tcgatcagct tacggtcgtc
       61 ataggtgccg acttcggcct cgggaaacga tctttggaac aggggaacca gcctgggatc
      121 gacggcgatt tgcaatttgc ctttggggcc gaccgcgcgc tggatgtcgg gcaggacgtc
      181 ggcgaacatg atctcgtcgc ccagtccctg ttcgccggtc accaggatgc gcctgccttc
      241 caaggattcg cccttccaca gcggcgcctt gatcatgtga tgcacatagg cccggaagcg
      301 ctcgttgttg cggatttcgt attcgcggaa gccttcctcc agcctgccca tgccgatcag
      361 acagacggcg cgcgaatgca gcgcctcgat gcgctcgtcg ggctcggcga caagcgccat
      421 cgcgttgtca taggcttcaa gcgcctcggc cagcctgccg agatgcgcca aagcgtagcc
      481 gaggttgtga tggaagcggg cgaaggccgg atcgagcctg atcgcctcgc gatagaacac
      541 caggctttcc tcgacgcgac cgtcctctgc cagcacggtg gcgagcgaat tccacaggat
      601 cgcctcgttc ggcaggcgca tgatggcgga acgcaaagtc tcgatcgcca tggtggaatc
      661 gcccatgtcg ctctggatgc tgcccagatt gttgtagccg agcggcgaat ccggccgggc
      721 ggcgatgtaa agccggaaca tctgttccgc gcccgctttc acattctggt tccatgccgt
      781 caggcccaga tcgagcaaaa ggtcgggatc gtcgggatcc agctggaaag cgcgctcgta
      841 ggtcgcgagg gcttcgcgta catggcccat cttctccagc gccattgcga gcacgtgaaa
      901 agcttgcgcg caggatggat cggccgtcgt ggcagcgagc gcccatttgg ccgcctgtgc
      961 cgcatcgccg cgtctgaacg cctcgatggc gcggcacaag cggccgttgg cttcgccgcg
     1021 cgctcctttg gtttcggttt cttcggcgag cgcctcgaat ttggcaagcg cttccagttt
     1081 tgcgtccatt cctgccagcg actcgatact gggtacggct gcggcgtgtg ccatatggct
     1141 tttcccccgg cgatttgagc cgccataaga acctttccat gcttaatccg aggtgaatgc
     1201 attcaattcc aatgcaaaga tattattaac tacggagcgc taaggtccgc ggacgcgaaa
     1261 gcctgtatcc cgacgagggg gtctttgatg ccgctgaaac tgtcgctgaa gccgggcgag
     1321 aagtttgtgc tgaacggcgc cgtgctgacc aacggcgaca agaagaagac cagcctggtg
     1381 atccagaaca aggcctgcat cctgcgcgaa aaggacatca tgcagccgga agaggcgacg
     1441 acgccggcgc gccacatcta tcttccgatc atgatgatgt atctcgatcc cgaaggcatc
     1501 gagacctatt acaataattt cgcgctgcgc atgacggagt tcatgggtgc catccagaac
     1561 cgcagcgctt tggcggtgtg catcgaaatc agccgcgacg tgatgagcgg cgattactat
     1621 aaggccctga tgttgtgcag gaaattgttc gaattcgagc aggaacggtt gaatcatgac
     1681 cctgaaagct tatcaaaacg cgttgcgtat agctgaagag ccgcgccaga ccgaataccg
     1741 gcttttcggc caggtcacgg gcgcgcttat caatgcgcaa aaagtccacg ccagcggcgg
     1801 cccgctggtc gagactatcg attggaatcg cagggtctgg cgcacgctcg ccgcggattg
     1861 tctggacgag accaatcagt tgccgaagga cgtgcgcgca cagatcgtgt cgctgtcgct
     1921 gtgggtcacc aaatactcga aggaggtgac gcgccagggc gcccctctcg atcctctgat
     1981 cacgatcaac cgtacgatca tggaagggtt gcgcggcgcc gcatagggcg cgcatcacga
     2041 caagcgcggg aaaatgccgg ccgcaggcgg ccggcatttt tcttttggcc cggtgagaat
     2101 tgcccaacgg cagaatatac ccggccgaaa cgatccacga tccgtcaact cttctggatt
     2161 ttcaattact tagtatatgc ctgagaatgg cgtgggagtt gcgacgcatg acggcggggg
     2221 caatctgccc tcaaagggca gaaggccgcg gggccgccag aacagaatgg agaatgaacg
     2281 gtgctaagcg tcaacaccaa tccgggcgca ttgaccgccc tgcaatatct gaacagcacg
     2341 aatgcgcaac ttcagacggc cgagaacgcc atcaactccg gtctgaaagt ggcttcggcg
     2401 aaggacaacg gcgccatttt cgccattgcc cagaacatgc gcggcaacgt ggcgggctat
     2461 tccgccgtca gcgattcgct gaaccgcgcc tcctcggtgg tcgataccgg cctgtcggcg
     2521 gcacaatccg tgtccgatct tctgatccag atgaagcaga acgcgctggc cgcggccgac
     2581 acgtcgctcg acgcgacgag ccgcgcctcg ctggccaggg atttccaggc attgtccgac
     2641 cagatcacca cgatcgtcga gaacgcgtcg ttcaacggca tcaacatcgt caacggcgcc
     2701 ggcacggggg tcagcgcctt ggcgtccgcc aacgaccccg accagacgat ctcggttgcc
     2761 gctcaggacc tgacggtcac gaatctggac gtggacgcgg cctatacgaa tcaggccagc
     2821 gcgaaagcga tggtcggcac ggtctccacg gcgatcgcca ccgtgaatac catcctgtcc
     2881 actttgtcga gcggttcgaa gaagttctcc atccaggcca gcttcgtctc caagctgtcc
     2941 gacacgctga cgagtggcat cggcaacctg gtcgacgccg acatggccaa ggaaagcgca
     3001 ttgctgacgt ccttgcagac caagcagcag ct
//
LOCUS       DASWPU010000109        14597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_25175,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000109 DASWPU010000000
VERSION     DASWPU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 14597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14597
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25175"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VGC27_02405"
     CDS             <1..216
                     /locus_tag="VGC27_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281456.1"
                     /translation="DKGQIDPTLGAGTTPRGIVDTNFAHAVTAAIADTRNKWAFLRSQ
                     LIARYGTRDGNLMICALTHDNAAKDCK"
     gene            278..469
                     /locus_tag="VGC27_02410"
     CDS             278..469
                     /locus_tag="VGC27_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281457.1"
                     /translation="MPLQNILILVRMSAGMFRDNVKKFTAYVNAALQTANAGARFYKG
                     KVGLRDIKRVAAAASYPSD"
     gene            complement(489..1628)
                     /gene="dnaJ"
                     /locus_tag="VGC27_02415"
     CDS             complement(489..1628)
                     /gene="dnaJ"
                     /locus_tag="VGC27_02415"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY0281458.1"
                     /translation="MSKRDYYETLGCQRGASIAEIKAAYRKLAMELHPDRNQGDRTSE
                     GKFKELNEAYDVLKDEQKRAAYDRFGHAAFEHGMGNGHGGGNPFDFAASFTDVFDDLF
                     GEMMGGRGRRRQNRGGDLRYNLQVSLEEAFRGRRVEIKVPSAVACEVCNGSGAAPGTH
                     AEQCSTCGGHGKVRATQGFFTVERTCPHCHGNGRVVKSPCKSCGGAGHVQKERTLAVD
                     IPPGVEEGTRIRLTGEGQAGWGGGPNGDLYVFLSVAQHEIFQRDGHDLHCLVPVSFVT
                     AALGGTVEVPTLDGGRAKITVPEGAQSGRQVRLRGKGMPVLRGGGMAGDLYIELMVET
                     PVKLSKKQKEILREFESAGDKGCQPQCDSFFSKVKAFWEGSATKH"
     gene            complement(1725..3743)
                     /gene="dnaK"
                     /locus_tag="VGC27_02420"
     CDS             complement(1725..3743)
                     /gene="dnaK"
                     /locus_tag="VGC27_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY0281459.1"
                     /translation="MGSLPPFLLVGGGLNGAFQEGQSPAARKEGKNMAKVIGIDLGTT
                     NSCVAVMEGSAPKVIENAEGARTTPSIVAFANDGERLIGQPAKRQGVTNPEYTFFAIK
                     RLIGRRFEDPMTQKDIGMVPYKIVKADNGDAWVGGRDDKRYAPSEISAFILQKMKETA
                     EAHLGEKVTQAVITVPAYFNDAQRQATKDAGRIAGLEVLRIINEPTAAALAYGLDKKN
                     TGVIAVYDLGGGTFDISVLEIGDGVFEVKSTNGDTFLGGEDFDQRVVNYLADEFKKET
                     GIDLRGDRLALQRLKDAAEKAKIELSSAMQTEVNLPFITADASGPKHLTIKITRAKLE
                     ALVDELIQRTLGPVKQALKDAGVTANQIDEVILVGGMTRMPKVQEAVKQLFGKEPHKG
                     VNPDEVVAIGAAIQGGVLKGEVKDVLLLDVTPLSLGIETLGGVFTRLIDRNTTIPTKK
                     SQIFSTAEDNQTAVTIRVFQGEREMAADNKLLGQFDLMGLPPAPRGVPQVEVTFDIDA
                     NGIVSVTAKDKATNKEQQIRIQASGGLSDADIKKMVSEAEAHAADDKKRRALAEAKNQ
                     ADALVHSTEVALKEHGGKIDAAERTAIEDAMASLKETAKNDDVEAIQQKTQALAQASM
                     KLGEAMYKASQAQASAEGAPKTNGEAPKDDNVVDAEFSEVDDNKKGAA"
     gene            complement(3911..4210)
                     /locus_tag="VGC27_02425"
     CDS             complement(3911..4210)
                     /locus_tag="VGC27_02425"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0281460.1"
                     /translation="MQKPTKLKKIPSFKSDRAAEAFVAKADLSQYDLSGGEFVRFEMK
                     RKDKSISLRLPELLLNDVRSHAKRVGVPYQRFIRMALERAVQPKPTPHQRRETTT"
     gene            complement(4188..4484)
                     /locus_tag="VGC27_02430"
     CDS             complement(4188..4484)
                     /locus_tag="VGC27_02430"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY0281461.1"
                     /translation="MEFSGFDWDAANRTKCQKHGVTIAAIESLFLRGVMILPDAGHSQ
                     KELRYRAIGHTEQGRAVFVVFTMRRSKGELLIRPISARTMHKKETEHYAETYKT"
     gene            complement(4566..5984)
                     /locus_tag="VGC27_02435"
     CDS             complement(4566..5984)
                     /locus_tag="VGC27_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0281462.1"
                     /translation="MDNTSLTRFMSGIWDQDIVPALADYIRIPNKSPMFDSEWEKHGH
                     MDKVVDLFAAWARGKIAGLPGATLEIVKPAGRTPLIFIEVPGKANGTVFMYGHLDKQP
                     EMTGWSEGMGPWTPVLKDGKLYGRGGADDGYAMFAALSALLALEEQNIAHARAVIAIE
                     AGEESGSPDLPFYIEHLAARIGVPDLIVCLDSGCGNYEQLWLTTSLRGIAAGKLTVRV
                     LTEGVHSGDASGIVPSSFRILRRLLSRIEDEATGAIKLDELFVTVPADRLEQARAAAS
                     TLGDEIFTKLPFAGATRPMAANRVEMILNRTWKPQLAVVGVDGYPPPESAGNVLLHFT
                     IAKLSMRLPPTCDAPAAVKVLKRSLEADPPYGAEVSFETETGQSGWNAPSLTSWLEAS
                     LAAASNAHFGKPVGFMGEGGSIPFMAMLGEKFPKTQFVVTGVLGPGSNAHGPNEFLHI
                     PFAKKLSACIADVLADHAKAHK"
     gene            complement(5992..8247)
                     /locus_tag="VGC27_02440"
     CDS             complement(5992..8247)
                     /locus_tag="VGC27_02440"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY0281463.1"
                     /translation="MTPDNRPSFTEEEALAFHAGAKPGKIEITPTKPMATQRDLSLAY
                     SPGVAVPVRAIAANPDAAYDYTAKGNLVAIISNGTAILGLGDLGALAAKPVMEGKAVL
                     FKRFADVDAIDLEIDTTDVDAFVNCVRYLGPTFGGINLEDIKAPDCFVIEEKLRELMN
                     IPVFHDDQHGTAIICTAGIINALHLRGCKLSDIKLVVNGAGAAGVACLELLKSMGLPP
                     ANATLCDSKGVVYRGRAAGMNQWKSAHATETKARSLADAMKDCDVFLGLSAKGALTKE
                     MVATMATAPIIFAMANPDPEIAPEEAKAVRPDAVVATGRSDYPNQVNNVLGFPYIFRG
                     ALDVRATTINEAMKIAAVEALASLAREDVPDEVAAAYRGSRPHYGPDYIIPVPFDPRL
                     ISRVSSAVAEAAIKSGVARRKIEDFDHYRYELSARLDPSATLFHQITASVRARPKRVV
                     FAEGEEECVIRAAAAFQHAGLGKALLVGREEIVRAGIKHAGLEGDTSLEICVPHSAAE
                     AAPYIDALYKRLQRHGGLYRDCVRLVTNDRNVYAASMLAAGDADAMVTGVTRNYTVAL
                     SDVRKVLDSPPGQRPIGVLIIFAKGRVIFAADTNVHDLPTSEQLADITIQAARVTRRL
                     GFTPRAALLASSTFGFPRTERSERIVEAVHILDRRQVDFEYDGEIAVDVALDREKLAL
                     YPFCRLTDTANVLIMPAVHSAAIATKLLQQIGGVSMMGPFLMGLEKPVQIVSLGAKMS
                     EIYNAALIAAL"
     gene            8355..11009
                     /gene="mutS"
                     /locus_tag="VGC27_02445"
     CDS             8355..11009
                     /gene="mutS"
                     /locus_tag="VGC27_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY0281464.1"
                     /translation="MNEQLPASAAGSGATPVMAQYLEIKAAHADYLLFYRMGDFYELF
                     FEDAAKASEALDIALTKRGKHLGDDIPMCGVPVHTAEQYLERLIRKGFRVAICEQVED
                     PALARKRGSKSVVRREVVRLVTPGTLTEDSLLEPSRANTLASLGRAGGEFALAAADMS
                     AGSFRVAAVAPGDLAVEIARLAPTELLAPDSLLTDAALAPVLNALKEGLTVLPAVKFD
                     SAAGERALKSHYAVAELAGFGSFSRAELSAAGALVAYLELTQKGKLPALQPLSRESSR
                     AFMAVDAATRRNLELTETLAGERRGSLLSTIDRTVTAAGARLIGARLAAPLTEVKKIV
                     SRQDAVSFFAADGDEREALREILRRAPDIARALARLSVARAGPRDLTALRDGISCARA
                     VRELLQAATECGEAGDAVRDLVECVAAISALNDKLNHLLVEEPPFYARDGGFVRAGAH
                     APLDEVRALRDESRRVIASLETSLREESGVPLKIKHNGVLGYFIEVASQHADKLMTAG
                     YRHRQTMGGAVRFSTDELSTLANRISQAADQALAIEKELFEEMSNEALAASQPLARIA
                     EALAVLDVAAALAELAVSKRHVRPKVDDSRAFAISRGRHAVVEAALEAAHAGFVPNDC
                     DLSSEKRLWLVTGPNMAGKSTFLRQNALTAIMAQMGAFVPADSAHIGIVDRLFSRVGA
                     GDDLAAGRSTFMVEMVDTSAILNQATEKSLVILDEIGRGTATFDGLAIAWATAEHLCL
                     KNKSRALFATHFHEMTALAERLPSLACVTMRVREWNDSIVFLHEVVPGVADRSYGIHV
                     AKLAGLPPSVVARAQEVLRALEEGREGHKPLARIDDLPLFNAAAPPPAKKKNALEEAV
                     RTADPDALTPKEALEFLYTLKRKLDSQP"
     gene            11320..11793
                     /locus_tag="VGC27_02450"
     CDS             11320..11793
                     /locus_tag="VGC27_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0281465.1"
                     /translation="MNASPSSHPRKFLVVVDKTPECKVALRFAARRAQHTGGRVSLLC
                     VAQTVDFQQWKGVEEIMTDEAHREAESLVYEAAKTVNELVGIVPELLIEHGVPAECLL
                     DLIGRDKDISILVLASGTSKDGPGPLVSLFASTVQAIPVTIVPGDLSDEAVDELA"
     gene            11903..12097
                     /locus_tag="VGC27_02455"
     CDS             11903..12097
                     /locus_tag="VGC27_02455"
                     /inference="COORDINATES: protein motif:HMM:NF016473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIG1 domain-containing protein"
                     /protein_id="HEY0281466.1"
                     /translation="MIGKIIILVALAAVAAILLAGIVVMAIGGEIGARWSNVLMRYRI
                     MAQAGALLLIALVVYFATAH"
     gene            12226..13668
                     /gene="leuC"
                     /locus_tag="VGC27_02460"
     CDS             12226..13668
                     /gene="leuC"
                     /locus_tag="VGC27_02460"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY0281467.1"
                     /translation="MKCSSRLKRDRKTMGKTLYDKIWAAHLVHEAPGESPVLYIDRHL
                     VHEVTSPQAFEGLRTAGRKARRPERALAVADHNVPTRDRAKGIADPESREQIATLENN
                     AREFGIDYLAMDDIRQGIVHIVGPEQGFTLPGTTIVCGDSHTSTHGAFGALAFGIGTS
                     EVEHVLATQTLLIPKSKNMRISVGGKLPLGVTAKDMALSVIARIGTAGGTGYVIEYAG
                     EAVSALSMEGRMTLCNMTIEGGARAGLVAPDEVTFAYIAGRPRAPKAAAFEAAVAFWR
                     TLKSDDDATFDAEIVLDAGEIAPMVTWGTSPEQALPVTGAVPDPAAVADENRRAGIER
                     ALAYMDLKPGTKLTDVKIDRVFIGSCTNGRIEDLRAAAAIARGKKVAAHVGAMVVPGS
                     GLVKEQAEQEGLDKIFLAAGFEWREPGCSMCLAMNADKLQPGERCASTSNRNFEGRQG
                     RGGRTHLVSPAMAAAAAIAGYLADVRLFQS"
     gene            13740..14216
                     /locus_tag="VGC27_02465"
     CDS             13740..14216
                     /locus_tag="VGC27_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281468.1"
                     /translation="MNLRIIAMGFAAFLLCGCTEADLDYATLYVVPGQAKESEPPQAA
                     QIESSELPPARRTENSAPPAAPTNNSESPLVVQTENREPPRAVPTENREPPAAPARTQ
                     AAPAPIPAEQAAEPDSWCQQIAKSSANEAARNGYDSSTQQRRAEKTYRQCVGSSGR"
BASE COUNT         2487 a         4573 c         4748 g         2789 t
ORIGIN      
        1 gacaagggcc agatcgatcc caccctcggc gccggcacca cgccgcgcgg catagtcgat
       61 acgaacttcg cccatgcggt gacggctgcc atcgccgaca ctcgcaacaa atgggccttc
      121 ctgcgttcgc agctcatcgc ccgttacggc acgcgcgacg gcaatctgat gatctgcgcc
      181 ctcacccacg acaacgcggc aaaggactgt aaatagaact tccgggtttg cgttttgaag
      241 cggcgcaggg ccaaccttcg ccgcttcagt tttgttcttg ccgctccaga acatcttgat
      301 tttggtgaga atgtcggccg gcatgttccg cgacaacgtc aagaagttca cggcttacgt
      361 gaacgcggca cttcaaacgg ccaatgccgg ggcccgcttc tacaaaggca aggtcggttt
      421 gcgcgacatc aagcgcgtcg ccgccgccgc ttcgtatccg tccgattgaa cggcttggcc
      481 catacggctc agtgcttggt cgccgagcct tcccagaagg ccttcacttt ggagaagaag
      541 ctgtcgcatt gcggctggca gcccttgtcg cccgcgctct cgaattcgcg cagaatctcc
      601 ttctgtttct tggacagttt gaccggggtt tccaccataa gttcgatata aaggtcgccg
      661 gccatgccgc cgccgcgcag caccggcata cccttgccgc gcaggcggac ctggcggccc
      721 gattgggcgc cctcgggcac ggtgatcttg gcgcgtccgc cgtcgagcgt cggcacctcc
      781 acggttccgc ccagcgccgc ggtgacgaac gacaccggca ccaggcaatg aagatcgtgg
      841 ccgtcgcgct ggaagatttc gtgctgggcg accgagagga aaacgtagag atcgccgttc
      901 ggcccgccgc cccagccggc ctggccttcg cccgtgaggc ggatgcgggt gccttcctca
      961 acccccggcg gaatatcgac ggccagcgtg cgttccttct gcacatggcc ggcgccgccg
     1021 caggatttgc aggggctttt gaccacgcgg ccgttgccgt gacagtgcgg acaggtgcgt
     1081 tcgacggtga agaaaccctg ggtggcgcgt accttgccgt ggccgccgca ggtcgagcac
     1141 tgctcagcat gagtgccggg ggcggcgccc gagccgttac aaacctcgca ggcgacggcg
     1201 gagggaacct tgatctcgac gcgccggccc cggaaagctt cttccagcga aacctggaga
     1261 ttgtatctga gatcgccgcc gcggttctgc cggcgccgcc cccggccgcc catcatttcg
     1321 ccgaatagat cgtcgaacac gtcggtgaag gaagcggcga aatcgaaggg gttgccgccg
     1381 ccatggccgt tgcccatgcc gtgctcgaag gcggcgtggc cgaagcggtc gtaagcggcg
     1441 cgcttctgtt cgtccttcaa gacgtcatag gcttcgttga gttccttgaa tttgccttcg
     1501 gaggtgcggt cgccctggtt gcggtcggga tgcagctcca tcgcaagttt gcgataggcc
     1561 gccttgatct cggcgatcga agcgcccctc tgacagccca gcgtctcgta atagtcgcgt
     1621 ttgctcatgg cagggtatca ggagccgggg atcgggaatc agacactgac gtaaccattt
     1681 gtactcgtcc catccgattc ccgatgacct atgcctgcct ccggctacgc cgcgcccttt
     1741 ttgttgtcgt cgacttcgga gaactccgca tccacgacat tgtcgtcctt gggcgcctcg
     1801 ccgttcgtct tgggcgcgcc ttcggcgctg gcctgggctt ggctggcctt gtacatcgcc
     1861 tcgccgagct tcatcgacgc ttgcgccaaa gcctgagtct tctgctggat cgcctcgacg
     1921 tcgtcgttct tggccgtttc cttcagcgac gccatggcgt cctcgatggc ggtgcgctcg
     1981 gccgcgtcga tcttgccgcc atgttccttc aacgccacct cggtcgagtg aaccaaggcg
     2041 tcggcttggt tcttggcttc ggccagcgcc cggcgcttct tgtcgtcggc ggcatgggct
     2101 tcggcttccg aaaccatctt cttaatgtcg gcgtcagata gaccacccga ggcttggatg
     2161 cgaatctgct gttccttgtt ggtggctttg tccttcgcgg tgaccgacac gatgccgttg
     2221 gcgtcgatgt cgaaggtcac ttccacctgc ggcacgccgc gcggggcggg cggaaggccc
     2281 atcaggtcga actggccgag cagcttgttg tcggccgcca tttcgcgctc gccttggaag
     2341 acgcggatgg tcacggcggt ctggttgtct tcggcggtcg agaagatctg gctcttcttg
     2401 gtcgggatgg tggtattgcg gtcgatcagg cgggtgaaca cgccgcccag cgtctcgatg
     2461 ccgagcgaca gaggcgtcac gtcgagcagc agcacgtcct tgacttcgcc cttcaagacg
     2521 ccgccttgga tggcggcgcc gatggcgacc acttcgtccg ggttgacgcc cttgtgcggc
     2581 tccttgccga acaattgctt caccgcttcc tgcaccttgg gcatgcgggt catgccgccg
     2641 accaggatca cttcgtcgat ttgattggcg gtgacgccgg cgtccttgag ggcctgcttt
     2701 accgggccga gcgtcctctg gatcagttcg tcgaccaagg cttccagctt ggcgcgggtg
     2761 atcttgatgg tgaggtgctt gggcccggaa gcgtccgccg tgatgaaggg cagattcact
     2821 tcggtctgca tcgcgctgga aagctcgatc ttcgcctttt cagcggcgtc tttcaggcgc
     2881 tgcaaggcga gccggtcgcc gcgcagatcg atgccggttt ccttcttgaa ttcgtcggcc
     2941 aggtaattga ccacgcgctg gtcgaaatcc tcgccgccca ggaaagtgtc gccgttggtg
     3001 gacttcacct cgaacacgcc gtcgccgatt tccagcacgg agatgtcgaa ggtgccgccg
     3061 ccgaggtcat agaccgcgat gacgccggta tttttcttgt cgagaccgta ggccaacgcc
     3121 gccgccgtcg gctcattgat gatgcgcagg acttcgaggc cggcgatgcg gccggcgtcc
     3181 ttggtggctt ggcgctgggc gtcgttgaaa taggccggaa cggtgatgac ggcctgcgtc
     3241 accttttcgc ccagatgggc ctcggccgtt tccttcatct tctgcaggat gaaggcggaa
     3301 atttcgctcg gcgcatagcg cttgtcgtcg cggccgccga cccaggcgtc gccattgtcg
     3361 gccttgacga tcttgtaagg caccatgccg atgtccttct gggtcatggg gtcttcgaag
     3421 cgccggccga tcagccgctt gatcgcgaag aaggtgtatt cggggttggt gacgccctgc
     3481 cgcttggcgg gctgtccgat cagccgctcg ccgtcattgg cgaaggcgac gatggagggg
     3541 gtggtgcgcg cgccctccgc attctcgatg accttggggg ccgagccctc catgaccgca
     3601 acgcacgaat tggtcgtgcc gaggtcgatg ccgatcactt tagccatgtt ttttccttcc
     3661 ttccgggcgg caggactttg gccttcctga aaagcgccgt taagcccgcc cccaaccaat
     3721 aagaatggcg gcaaagagcc caaaagccct taccgcaccc ggggggtata tagggtgggg
     3781 gtccccgcct cgcaaggtcg cgaaattgtg atagggcgcg gaaaatagcc ctaagtcact
     3841 ggaacgaaag gggatcgagg ggcctgcgca gcttcgccgc gtgaaggccc tcctcggcgg
     3901 ttttcccgtc ttaggtggtc gtctcgcgcc gctgatgtgg ggtgggcttc ggctgcacag
     3961 cccgctctaa ggccatgcgg ataaagcgct gataaggcac accgacccgc ttggcgtggc
     4021 tacgcacgtc gttcaagagc aattcgggca ggcgcagact aatcgattta tccttgcgct
     4081 tcatctcgaa gcgtacgaat tcccccccgg aaagatcgta ttgcgacaaa tcggccttgg
     4141 cgacgaacgc ctcggctgcg cggtcgctct tgaagcttgg aattttctta agttttgtag
     4201 gtttctgcat aatgctcggt ttccttttta tgcatggtgc gggcgctaat cgggcggatc
     4261 aatagttcgc ctttgctgcg gcgcatcgtg aacaccacga ataccgcccg gccttgctcg
     4321 gtgtgcccga tagcccggta tcgaagctct ttttgcgagt gtccggcatc gggcaagatc
     4381 atcacgcccc ggagaaacaa gctttcgatg gcagcgatcg tcacgccgtg cttttggcac
     4441 ttcgttcggt ttgcggcgtc ccagtcaaat cccgagaact ccatcaggcc accccatcat
     4501 tgtatataca attgcatata caatcgtcaa gcctcgcatt ccggcgcctt gtgcggcttt
     4561 agggactatt tgtgtgcctt ggcgtggtcc gccagcacgt ccgcgatgca ggccgagagc
     4621 ttcttggcga acgggatgtg caggaactcg ttgggcccgt gggcgttgga gcccggtccc
     4681 aacacccccg tcaccacgaa ctgggtcttg gggaatttct cgcccagcat ggccatgaag
     4741 gggatcgagc cgccctcgcc catgaagccc accggcttgc cgaaatgggc gttcgaggcg
     4801 gcggccagcg aagcctccag ccaggacgtc agcgacggcg cgttccatcc cgattgcccg
     4861 gtttcggttt cgaagctcac ttcggcgccg taaggcggat cggcttcgag gctgcgcttc
     4921 agcactttca cggcagcggg cgcatcgcag gtcggcggca ggcgcatgga aagcttcgca
     4981 atggtgaagt ggagcagcac attgccggcg ctctcgggcg gcggatagcc gtccactccg
     5041 accaccgcga gctgcggctt ccaagtgcgg ttgaggatca tctccacgcg gtttgcggcc
     5101 atcggcctgg tggcgcccgc gaagggcagc ttggtgaaga tttcgtcgcc cagagtggag
     5161 gcggcggcgc gcgcctgctc cagccggtcg gccggcacgg tgacgaacag ttcgtcgagc
     5221 ttgatcgcgc ccgtggcttc gtcctcgatg cggctgagga gccggcgcag gatgcggaac
     5281 gacgacggca cgatgccgct ggcgtcgccc gaatgcaccc cttcggtgag cacgcgcacc
     5341 gtcagcttgc ccgccgcgat gccgcgcagc gaggtggtca gccaaagctg ctcgtaattg
     5401 ccgcagcccg aatccagaca gacgatcaaa tccggcaccc cgatgcgcgc cgccagatgc
     5461 tcgatataga agggcaggtc gggcgaaccc gattcttcgc cggcctcgat ggcgatcacc
     5521 gcgcgtgcgt gcgcgatgtt ctgctcttcg agcgcgagca gcgccgagag cgccgcgaac
     5581 atggcatagc cgtcgtcggc gccgccgcgg ccgtaaagct tgccgtcctt gagcacgggc
     5641 gtccacggcc ccatgccttc ggaccagccg gtcatctcgg gctgcttgtc gagatggccg
     5701 tacatgaaca cggtgccgtt cgccttgccc ggcacctcga tgaagatcag cggcgtgcgc
     5761 cccgcgggtt tgacgatctc cagcgtcgcg ccgggaagcc ctgcgatctt gccgcgcgcc
     5821 caagccgcga acaggtcgac caccttgtcc atgtggccgt gcttttccca ttcggaatcg
     5881 aacatcggcg atttgttggg gatacggata tagtcggcga gcgccgggac gatgtcctgg
     5941 tcccagatgc cgctcatgaa gcgcgtcagc gatgtgttgt ccatggctct ttcacagggc
     6001 cgcaatcagg gcggcgttgt agatttcgct catcttggca ccgagcgaga cgatctgcac
     6061 gggtttttcc agccccatca ggaacggccc catcatggag acgccgccga tctgttgcag
     6121 cagcttggtg gcgatggcgg cggaatggac cgcgggcatg atcagcacgt tggcggtatc
     6181 ggtcagccgg cagaagggat agagcgccag tttctcgcgg tcgagcgcca cgtccaccgc
     6241 gatttcgccg tcatattcga aatcgacctg gcgccggtcg agaatatgca cggcctcgac
     6301 gatgcgttcg ctgcgctcgg tgcggggaaa gccgaaggtg gaggatgcga gcagcgccgc
     6361 gcgcggcgtg aagcccagcc tgcgcgtgac gcgcgccgcc tggatggtga tgtcggcaag
     6421 ctgctcggag gtgggcaaat cgtggacgtt ggtgtcggcc gcgaaaatca cccgcccctt
     6481 agcgaagatg atcagcacgc cgatcggccg ctggccggga ggcgagtcga gcactttgcg
     6541 cacgtcgctt aacgcgacgg tgtagttgcg cgtcaccccg gtgaccatgg cgtcggcgtc
     6601 gcccgccgcc agcatcgagg cggcgtagac gttgcggtcg ttggtgacca ggcgcacgca
     6661 gtcgcgataa agcccgccgt ggcgctgcag ccgcttgtag agcgcgtcga tataaggggc
     6721 cgcttcggcg gcggaatgag gcacgcagat ttcgagcgac gtgtcgcctt cgagaccggc
     6781 gtgcttgatg ccggcgcgga cgatctcttc gcgccccacc agcagggctt tgcccagtcc
     6841 ggcatgctgg aaagcggccg cggcgcggat gacgcattct tcctcgcctt cggcgaacac
     6901 cacccgctta ggccgggcgc gcacgctggc ggtgatctgg tggaacaggg tcgccgaagg
     6961 gtcgagccgc gccgaaagtt cgtagcggta atggtcgaaa tcctcgatct tgcgccgtgc
     7021 cacgcccgac ttgatcgcgg cttccgcgac ggccgaggat acccggctga tcaggcgcgg
     7081 atcgaacggc accggaatga tgtagtcggg gccgtaatgg gggcgcgagc cgcgataggc
     7141 cgccgccact tcgtcgggca cgtcctcgcg cgccagcgac gccagcgctt ccaccgcggc
     7201 gatcttcatc gcttcgttga tggtggtggc gcgcacgtcc agcgcgccgc ggaagatgta
     7261 gggaaagccc aacacgttgt tcacctggtt ggggtaatcg ctgcgtccgg tcgccacgac
     7321 ggcgtcgggg cgcaccgctt tggcctcttc gggcgcgatt tcgggatcgg gattggccat
     7381 ggcgaaaatg atcggggccg tggccatggt cgcgaccatt tccttcgtca gcgcgccctt
     7441 cgccgacagg ccgaggaaga cgtcgcaatc cttcatcgca tcggcgaggc tgcgcgcctt
     7501 ggtttcggtg gcgtgcgcgc tcttccactg gttcatcccc gccgcgcggc cgcgatagac
     7561 cacgcccttg gaatcgcaca gggtcgcgtt ggcgggcggc aggcccatcg atttgagaag
     7621 ctccaggcag gcgacgcccg ccgcgccggc accgttcacc accagcttga tgtcggaaag
     7681 cttgcatccc ctcagatgca gcgcgttgat gatgccggcg gtgcagatga tcgcggtgcc
     7741 gtgctggtcg tcgtggaaga cggggatgtt catcagttcg cgcagcttct cctcgatgac
     7801 gaagcagtcc ggcgccttga tgtcctccag attgatgccg ccgaaggtgg ggccgagata
     7861 gcgcacgcaa ttgacgaagg cgtccacgtc ggtggtgtcg atttccaggt cgatggcgtc
     7921 cacgtcggcg aagcgcttga acaggacggc cttgccctcc atcaccggct tggcggcgag
     7981 cgcccccaga tcgcccaggc ccaggatcgc ggtgccgttg gagatgatgg cgacgaggtt
     8041 ccccttggcc gtgtaatcgt aggccgcgtc cgggttcgcg gcgatggcgc gcaccggcac
     8101 cgccacgccc ggcgaatagg caaggctgag gtcgcgctgc gtcgccatcg gcttggtcgg
     8161 ggtgatctcg attttccccg gcttggcgcc ggcgtgaaaa gccagagctt cttcttcggt
     8221 gaaggaaggg cggttgtcgg gggtcatggg cgattcccaa atccgtgctt ttccacagga
     8281 tatcaggttg cccccccttt gcacccgccg aagtcgcaac ccagggcggg ctttgcatct
     8341 gctaggctcg cccgatgaac gagcagcttc cagcgtcggc ggcgggcagc ggggcgaccc
     8401 ccgtgatggc gcaatatctt gagatcaagg ccgcccacgc ggattatctt ctgttctacc
     8461 ggatgggcga tttctacgaa ttgttcttcg aagatgccgc caaggcgtcc gaggccctcg
     8521 atatcgccct gaccaagcgc ggcaagcatc tgggcgacga catcccgatg tgcggcgtgc
     8581 cggtgcatac cgccgagcaa tatctggaac ggctgattcg caaaggcttc cgcgtcgcca
     8641 tttgcgagca ggtggaagac ccggcgctgg cccgcaagcg cggctccaaa tccgtcgtgc
     8701 ggcgcgaggt cgtgcgcctg gtgacgcccg gcaccttgac cgaggattcg ctgctggagc
     8761 cgtcgcgcgc caacacgctg gcgagcctgg ggcgggccgg cggcgagttc gcgctcgccg
     8821 ccgccgacat gtcggcggga agtttccgcg tcgccgcggt cgcgcccggc gatctggcgg
     8881 tggagatcgc ccggctggcg ccgaccgaac ttctggcccc cgactcactg ctgacggatg
     8941 cggcgctggc gccggtgctg aatgcactga aggaaggatt gacggtgctg ccggcggtga
     9001 aattcgattc cgcggcgggc gaacgggcgc tgaaaagtca ttacgccgtc gccgagcttg
     9061 ccggcttcgg cagtttttcg cgcgcggaat tgtcggcggc gggcgcgctg gtcgcttatc
     9121 tcgaactgac gcagaagggc aagcttccag cgctgcaacc cttgtcgcgc gaatcctcgc
     9181 gcgccttcat ggccgtcgat gcggcgacgc ggcgcaatct cgaactgacc gagacgctgg
     9241 ccggcgaacg gcgcggcagc cttctttcga ccatcgaccg cacggtgacg gcggcgggcg
     9301 cgcgtctcat cggcgcgcgg ctggcggcac cgctgaccga ggtgaaaaaa atcgtctcgc
     9361 gccaggacgc ggtttccttc ttcgcggcgg acggtgacga gcgcgaggct ttgcgcgaaa
     9421 ttctgcgccg cgcccccgat atcgcccgcg cgctggcgcg cctttcggtg gcgcgcgctg
     9481 gcccgcgcga tctgaccgcc ttgcgcgacg ggatttcctg cgcccgcgcc gtgcgcgagc
     9541 ttctacaggc agcgacggag tgcggcgaag cgggcgatgc cgtgcgcgat ctggtggagt
     9601 gcgttgccgc gatttcggcg ctgaacgaca agctgaacca tcttctggtg gaagagccgc
     9661 ccttttacgc ccgcgacggc ggtttcgtgc gggccggcgc ccatgcgccg ctcgacgagg
     9721 tgcgcgcgct gcgcgacgaa tcgcgccggg tgatcgcttc gctggaaaca agcctgcgcg
     9781 aggaaagcgg cgtcccgctc aagatcaagc ataacggcgt gctcggctat ttcatcgaag
     9841 ttgccagtca gcacgccgac aagctgatga ccgcgggcta tcgccatcgc cagaccatgg
     9901 gcggggcggt gcgcttttcg accgacgaac tctccacctt ggcgaaccgg atctcgcagg
     9961 ccgccgatca ggccctcgcc atcgaaaagg aattgttcga ggagatgtcg aacgaggcgc
    10021 tggcggcgtc gcagcccttg gcgcggatcg ccgaagcgct ggccgtgctc gacgtggctg
    10081 ctgcgctcgc cgagctggcg gtctcaaagc gtcacgtccg tcccaaagtc gacgacagcc
    10141 gcgccttcgc gatttcgcgc ggccgccacg cggtggtgga ggcagcgctg gaagcggcgc
    10201 atgcgggctt cgtgcccaac gattgcgacc tgtcttcgga gaagcgtttg tggctggtga
    10261 ccggccccaa catggcgggc aaatcgacct tcctgcgcca gaacgctctt acagcaatca
    10321 tggcgcagat gggcgccttc gtgcccgccg acagtgctca tatcggcatc gtcgatcggc
    10381 tgttcagccg cgtcggcgcc ggtgacgatc tcgccgccgg ccgttccacc ttcatggtcg
    10441 agatggtgga tacttccgcc atcctcaatc aggcgacgga gaagagtctg gtgatcctcg
    10501 acgagatcgg ccggggtacg gcgaccttcg acggcctcgc catcgcctgg gccaccgccg
    10561 agcatctatg cctgaagaac aagtcgcgcg ccctcttcgc cacccatttc cacgagatga
    10621 cggcgctggc cgaacgcttg ccgtcgctcg cttgcgtcac catgcgggtc agggaatgga
    10681 acgacagcat cgtgttcctg cacgaagtgg ttcccggcgt cgccgaccgt tcctacggca
    10741 ttcatgtcgc caagctggcg ggacttccgc cttccgtcgt ggcgcgggcg caagaggtat
    10801 tgcgcgcctt ggaggaaggc cgcgaagggc ataagccgct cgcccgcatc gacgatctgc
    10861 cgctgttcaa tgccgccgcg ccgccgcccg ccaagaagaa gaacgcgctg gaagaagccg
    10921 tccgcactgc cgatcccgac gcgctgacgc cgaaagaagc attggaattc ctctacactt
    10981 tgaaacgaaa attggattcg cagccatgaa gcgcaatccg gcgaagtggg aaccggttcg
    11041 ccgctagtgt cgtggtttcg aagttcgcag catgcgcact atcgggctgg aagcgaactt
    11101 cgaaaccaaa acgacactag gattcaatag gttgctagtg tcctttcgat tccgaaattc
    11161 gcccgaagcc gatataaaag atggtgcgaa tttcggaaat ctggacacta gattgcgcga
    11221 aaatcaagga atctggagca tggtccgatt caaccagatt aatacatgct ccaatgcggc
    11281 cttgccgcgt tgctcgcgat ggtgtcaggc tccaggatca tgaacgcttc gccttcgtcc
    11341 catccgcgca aattcctggt cgtcgtcgac aagacgccgg aatgcaaagt ggcgttgcgc
    11401 tttgccgcgc gccgcgccca gcacaccggc gggcgcgtct cgcttttgtg cgtggcgcag
    11461 acggtcgatt tccagcaatg gaaaggggtc gaggaaatca tgacggacga ggcgcatcgc
    11521 gaagcggaaa gcttggtcta tgaggcggcc aaaaccgtca acgaactggt cggcatcgtg
    11581 cccgaactgt tgatcgagca tggcgtgccg gccgaatgcc tgctcgacct gatcggccgc
    11641 gacaaggata tttccatcct ggtgctggcc tcgggaacct ccaaggacgg ccccggcccg
    11701 ttggtcagcc tgttcgcctc gaccgtccag gccattcccg tcaccatcgt cccgggcgat
    11761 ttgagcgacg aggccgtgga cgaattggct tgaccggcaa ggccttagca cgaactcgga
    11821 aggcgaattg cggcccttgc gccggttggc gggcagggac gatagttgcc gggcgcgagg
    11881 caaaggaacg caaggggacg ccatgatcgg aaaaatcatc attctggtgg ccttggcggc
    11941 cgtcgccgcc attttgctgg ccggcatcgt ggtgatggcg atcggcgggg agatcggcgc
    12001 gcgctggtcg aacgtgttga tgcgctaccg catcatggcg caggcggggg cgcttttgct
    12061 gattgccctg gtggtctatt tcgcgaccgc ccattaaccg ctgggtcttg cgcgcctgcg
    12121 cccgctggtg ttgttagagc gttttcagga aaagtggaaa ccggttttcc gtccgaaaac
    12181 gcgacaaact aagaatctag agcatttcat cgtttccgtg aaacggtgaa atgctctagc
    12241 cgcctgaaac gggaccgcaa gaccatgggc aagacgcttt acgacaagat ctgggccgcg
    12301 catctggtgc atgaggcgcc gggggaatcg cccgttctct atatcgaccg ccatctggtg
    12361 cacgaggtga ccagtccgca ggcgttcgag ggtctgcgca cggccgggcg caaggcgcgc
    12421 cgtcccgagc gggcgcttgc cgtcgccgac cataatgtgc cgaccaggga ccgcgccaag
    12481 ggcattgccg atcccgaatc ccgcgaacag atcgccaccc tggaaaacaa cgcgcgcgaa
    12541 ttcggcatcg actatctggc gatggacgac atccgccagg gcatcgtcca catcgtcgga
    12601 ccggagcagg gtttcaccct gccgggcacc accatcgtct gcggcgattc ccacacctcc
    12661 actcacgggg ccttcggggc gcttgccttc ggcatcggca ccagcgaagt cgagcatgtg
    12721 ctcgccaccc agactctgtt gattccgaaa tcgaagaaca tgcgcatcag cgtgggcggc
    12781 aagctgccgc tcggcgtcac cgccaaagac atggcgctct ccgtcatcgc caggatcggc
    12841 accgccggcg gcaccggcta tgtcatcgaa tatgccggcg aggcggtcag cgcgctctcg
    12901 atggaagggc ggatgacact ctgcaacatg accatcgaag gcggcgcgcg cgcgggtctg
    12961 gtggcgcccg acgaagtcac cttcgcctat atcgcgggcc gtccccgcgc gcccaaagcg
    13021 gccgcgttcg aagcggcggt ggcgttctgg cgcacgctga agtccgacga cgacgcgaca
    13081 ttcgatgccg aaatcgtgct cgacgcaggt gaaatcgcgc cgatggtgac ctggggcacc
    13141 tcgcccgaac aggcgctgcc ggtgaccggc gcggtccccg atcccgccgc tgtggccgac
    13201 gagaaccggc gcgcgggcat cgaacgggcg ctcgcttaca tggatttgaa gcccggcacc
    13261 aagctgaccg acgtgaagat cgaccgtgtt ttcatcggct cttgcaccaa cggacggatc
    13321 gaggatttgc gtgccgccgc cgccatcgcc aggggcaaga aggtcgccgc ccatgtcggc
    13381 gccatggtgg ttcccggttc cggtctggtg aaggaacaag ccgaacagga aggtctggat
    13441 aagatttttc tcgctgcggg cttcgaatgg cgcgagccgg gctgctcgat gtgtctggcc
    13501 atgaacgccg acaagcttca gccgggcgag cgctgcgcct cgacctcgaa ccgcaatttc
    13561 gaaggccgcc aggggcgcgg cgggcgcacc catctggtgt cgcctgccat ggccgccgcg
    13621 gccgcgatcg cggggtattt ggcggatgtg aggttgtttc aatcttgatt gtcatggctg
    13681 cggcaagcca ggcagaatgt tgaactaggg tagggcgcat ttatggaagc ggcggcatta
    13741 tgaatcttcg catcatcgca atgggttttg ccgcttttct gctctgcggt tgtaccgaag
    13801 ccgatttgga ctatgccacg ctgtacgtgg tgcctggaca ggctaaagaa agcgagccgc
    13861 cgcaggccgc acagattgaa agcagtgagc tgccgccggc cagacggacg gagaacagcg
    13921 caccgcctgc cgcaccgacc aacaacagcg agtcgccatt ggtcgtacag actgagaacc
    13981 gcgagccgcc gcgcgccgta ccgactgaga accgcgaacc gccagccgct ccggcccgta
    14041 cgcaggcggc tcccgcacca attccggcgg aacaggccgc cgagccggat tcctggtgcc
    14101 agcagatcgc caaatcgtcc gcgaacgaag cggcccgcaa cggctacgat tcgtcgaccc
    14161 agcagcgccg ggctgaaaag acctatcggc aatgcgttgg atcttccggc cgttagccgc
    14221 ggaatcgaag cggcgcggcg ctggcgtgct cgtcatgcgg cacgacgggg attgtgtgcc
    14281 gctgctgcga ttgcgcgacc tttggcggat gggaaatcac cgcggctccc gcgtcgcaat
    14341 gtctgtaagt tgtgctagtg tcgtggtttc gaagttcgca gcaagcgcac tatcgggctg
    14401 gaagcgaact ttgaaaccaa aacgacacta ggattcaata ggttgctagc gtccttcgat
    14461 tccgaaattc gcccgaagcc gatataaaga tggtgcgaat ttcggaatcg ggacactagg
    14521 gttcaacgaa tttcggaggt ggggcactat gaacgttcgc gttatcgctg tgtgttttgc
    14581 ggctttcctg ctttgcg
//
LOCUS       DASWPU010000110         5491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_43787,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000110 DASWPU010000000
VERSION     DASWPU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43787"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VGC27_02470"
     CDS             <1..1378
                     /locus_tag="VGC27_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY0281469.1"
                     /translation="PDLLLLDEPTNYLDLEGVMWLENFLQRYRGTVLIVSHDRDLLNT
                     AAEFILHLEHGKLSLYSGGYDKFVEARAQKRALDAAFAKKQDAARKHLQAFVDRFRYK
                     ASKASQAQSRIKMLERLQTVDIPVDEHTAPIRLPKATPASPPLITMDHASVGYEPGKP
                     VLTNLSLRFDSDDRIALLGKNGNGKSTLAKLLAGKLEPMSGELVRAKKLVPGYFAQHQ
                     LEELDGALTPIQALGHLRPKLTMQELRTQLGGFGFSADKAQTRTANLSGGERARLMLA
                     LATLDKPNLLILDEPTNHLDIDARGELLAALNDFDGAVVLVSHDRRLIEATADRLLLV
                     ANGRVLPFDGDLDDYRRFLLADESAHNEPEVKRSKEDARREAAERRRQLKPLKDRIDA
                     AEHQIAALTAEIKKLDSTLADPLLFVHDPKKGSSVSKKRADAVRKLAAAETSWLQAHE
                     AYEAANRE"
     gene            1553..1954
                     /locus_tag="VGC27_02475"
     CDS             1553..1954
                     /locus_tag="VGC27_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281470.1"
                     /translation="MAALFIAASVRASAYCSDTDAARAIRIVYTIGTWENLNSYVRKY
                     RDCDDGAVAEGNSDRVADLLARRWDTIGALVELTNGDPRFERFVLYHVDSLMRPVQAQ
                     AILDNVRNHCPADAAELCRKIEKEVLASTKQ"
     gene            complement(1974..2729)
                     /locus_tag="VGC27_02480"
     CDS             complement(1974..2729)
                     /locus_tag="VGC27_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281471.1"
                     /translation="MPVLGFGFLFSLLCAFLCAFHVYRTGRPYLWLLVIFLLPGGLGP
                     LIYVAYVLLFDTTDSRSVRRFADNVANAADPGRAYREKLREVERVGSADAKRALAEEC
                     IKRGRFQDAVELYQGAMSGPLGDSDAVLLRGLARARLLAGDGQGAVEAFENLKRMDRA
                     AFDADVELDYARALALVGRVDDALYQYETVVPRYPGEEARCRFALLLKSVGQTERARA
                     LFTEIIKSVRGAPGYYRRRQREWYRIARKQLNG"
     gene            complement(2786..3247)
                     /locus_tag="VGC27_02485"
     CDS             complement(2786..3247)
                     /locus_tag="VGC27_02485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004347.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY0281472.1"
                     /translation="MTDVLFYHLERRALDDVLPGLIERTLERGWRALIRAESAERADA
                     IDTRLWTFNDGFLPHAQTGDGDPARQPVLITVEEGNPNAANVLFLVGGAAPPAWDGAA
                     AKAFTRIVLLFDGRDPAAVETARAAWKDAKSAGNEVTYWKESPTGKWEKQG"
     gene            complement(3321..4508)
                     /locus_tag="VGC27_02490"
     CDS             complement(3321..4508)
                     /locus_tag="VGC27_02490"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0281473.1"
                     /translation="MSAKCHCQNHDSSENGLSRRALIETGAALGAVAALSPEAMAASP
                     KLKTIPATGYAAPSATGKLAPFHFKRRDTGPKDVLIDILYCGVCHSDIHTARGEWPGT
                     RYPCVPGHEILGRVVKVGDGVTRFKKGDMAAVGCLVDSCGHCASCNEGLEQFCENGAT
                     LTYNSDDKLLGTPTYGGYSNLVTVTEHFAVKVPDGMNAAGAAPLLCAGITTYSPLRHW
                     KVGPGTKVGIVGIGGLGHVGVKIAAAMGAEVTMITTTPAKMADAARLGAKDALLSTDE
                     DAMHKRQGTFDFLLSTIPCTHPVMPYVQLLRRDGTLVVVGALDHKSTEVYLATLVIQR
                     KTVAGSVIGGLPETQEMLDFCAQHNIVADADIIPISRINEAYDRVKAKDVRYRFVIDM
                     ASI"
     gene            complement(4683..>5491)
                     /locus_tag="VGC27_02495"
     CDS             complement(4683..>5491)
                     /locus_tag="VGC27_02495"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY0281474.1"
                     /translation="VGQGSERESQLVVMQWNGGRKKKDSPVAFVGKGVCFDSGGLSLK
                     TGAGMMGMKGDMGGAAAVVGVMRALAARKARVNAVGVIGLVENMPDGKAQRPDDVVKS
                     MSGQTIEVLNTDAEGRLVLADALWYAQTRFKPKFVIDLATLTGAILVALGAEHAGLFS
                     NDDKLSERLAEAGKEVGEPVWRLPLGKAYDKLLRSKIADMKNIGGPSAGSITAAQFLQ
                     RFVTDKTPWVHLDIAGVAWQDNERKPLIPSWGTGWGVRILDRLVAENYES"
BASE COUNT         1041 a         1772 c         1700 g          978 t
ORIGIN      
        1 gccggacctc ttgctgttgg atgaaccgac caactatctc gacctcgaag gcgtgatgtg
       61 gctggagaac ttcctgcagc gctatcgcgg cacggtgctg atcgtcagcc acgaccgcga
      121 cctgctcaac acggcggcgg agttcatcct gcatctcgaa cacggcaagc tgtcgctcta
      181 cagcggcggc tacgacaagt tcgtggaggc ccgcgcccag aaacgcgcgc tggatgcggc
      241 cttcgccaag aagcaggacg ccgcgcgcaa gcatttgcag gccttcgtgg accgcttccg
      301 ctacaaggcc tcgaaggcga gccaagccca aagccgcatc aagatgctgg agcggctaca
      361 aaccgtggac attccggtgg acgagcacac cgcgcccatc cgcctgccga aggccacgcc
      421 ggccagtccg ccgctgatca cgatggacca tgcgtcggtc ggctacgagc cgggcaagcc
      481 ggtactcacg aatctctccc tgcgcttcga ctccgacgac cgcatcgcgc ttctgggcaa
      541 gaacggcaac ggcaaatcga ccttggcgaa actgctggcc ggcaagcttg agccgatgtc
      601 gggcgaactg gtgcgcgcca agaagctggt gcccggctac ttcgcccagc accagttgga
      661 ggaattggac ggcgcgctga cgccgatcca ggcgctcggc catttgcggc cgaagctcac
      721 gatgcaggag ttgcgcacgc agctcggcgg ctttggcttc agcgccgaca aagcccagac
      781 cagaaccgca aacctttccg gcggcgaacg cgcgcggctg atgctggcgc tggcgacact
      841 cgacaagccc aacctgctga tcctggacga gcccaccaac catctggaca tcgatgcgcg
      901 cggcgaattg ctggcggcgc tcaacgattt cgacggcgct gtcgttctgg tcagccacga
      961 ccgcaggttg atcgaggcga cggcggatcg gctgctgctg gtggcgaatg ggcgcgtcct
     1021 gccgttcgac ggcgacctcg acgattaccg tcgcttcctg ctcgccgacg aatccgcgca
     1081 taacgagccg gaagtcaagc ggtccaagga agacgcgcgg cgcgaagccg ccgagcgccg
     1141 gcgccaactg aagccgctca aagacaggat cgacgcggcg gaacatcaga tcgcggcgct
     1201 gaccgccgag atcaagaagc tcgattccac gctcgcggac ccgcttttat tcgtgcatga
     1261 cccgaaaaag ggatcatccg tgtcgaagaa gcgcgccgac gccgtacgta agctcgccgc
     1321 cgccgaaaca agctggcttc aggcacatga ggcctacgaa gcggcgaata gagaataaga
     1381 taaagggtct tgcacgctca cggcggcctc gggcaattcg ctgcaccgtt ctgaaaggac
     1441 gaccggccgc tgatcggcta taattgctta ggataaacgt ccggcaccgt ggcattgccg
     1501 ccggagttgg gaggagaaaa cgagcatgcg taccatatgc ttttcgtttt tcgtggcggc
     1561 cctgttcatt gcggcctcgg tgcgcgcttc ggcctattgc agcgacactg acgccgcgcg
     1621 cgctatacga atcgtctata cgatcggcac ctgggaaaat ctcaatagct acgtgagaaa
     1681 atacagggac tgcgatgacg gcgctgtcgc cgaaggcaat tccgacaggg tggccgatct
     1741 tctggcgcgc cgctgggaca cgattggcgc gttggtggaa ctgacgaacg gtgatccgag
     1801 attcgagcgc tttgttctct accatgtgga ttcgctgatg aggcccgttc aagcccaggc
     1861 cattctcgac aacgtccgca accattgccc tgccgacgcc gccgagcttt gcagaaagat
     1921 agaaaaggaa gtgctggcat caacgaagca atgaacgcgg cgctgattgc ccttcatccg
     1981 ttcaattgtt tccgtgcgat gcggtaccat tcgcgttggc ggcggcgata atagccgggc
     2041 gcgccgcgga cggatttgat gatttcagta aacagcgccc gcgcgcgctc cgtctgcccc
     2101 accgatttca gcagcagggc gaagcggcag cgtgcctcct cgccgggata gcgcggcacg
     2161 acggtttcat actggtacag cgcatcgtcc acccggccga ccaaggccag ggcgcgcgca
     2221 taatcgagtt ccacgtcggc gtcgaaggca gcccggtcca tccgtttgag attctcgaac
     2281 gcctccacgg cgccttggcc gtcgcccgcg agcagcctgg cgcgcgccag gccgcgcagc
     2341 agaaccgcgt cgctgtcccc cagcgggccg ctcatcgcgc cttgataaag ttcgaccgca
     2401 tcctggaacc gcccgcgctt gatgcattcc tcggccagcg cgcgcttggc gtcggcggag
     2461 ccgactctct ccacctcgcg cagcttctcg cgataggcgc ggccgggatc ggcggcattg
     2521 gcgacattgt ccgcgaaacg gcgcacggac cgggagtccg tcgtgtcgaa cagcagcacg
     2581 taggcgacat agatcaacgg cccaaggccg cccggcagga ggaagattac caacagccat
     2641 aggtatggcc gcccggtgcg atagacatga aaggcgcata aaaaggcgca taaaagggag
     2701 aacaggaaac cgaaaccgag tactggcaag ggcgcatccg cctggacagg ttcccagccc
     2761 tgataggcgt cttgggcggg tcaaatcaac cctgcttttc ccattttccc gtggggcttt
     2821 ctttccaata tgtaacctcg ttgccggcgg atttggcgtc cttccaggcg gcgcgcgcgg
     2881 tttcgacggc ggcgggatcg cgcccgtcga acagcagcac gatgcgggtg aacgctttcg
     2941 cggccgcacc gtcccaggcg ggcggcgccg caccgccgac caggaacaga acattggcgg
     3001 cgttcggatt gccctcctcc accgtgatca gcaccggctg gcgcgcagga tcgccgtcgc
     3061 ccgtctgggc atgcggaagg aagccgtcgt tgaaggtcca cagccgcgtg tcgatggcgt
     3121 cggcgcgctc ggcggattcg gcgcggatca gcgcccgcca gccgcgctcc agcgtgcgct
     3181 cgatcagccc gggcagcaca tcgtccagcg cccggcgttc gaggtggtag aaaagaacat
     3241 cggtcatagg acaagcttat acgtctgccc tcgggagagg tcgatccggt cttcccgagg
     3301 gcagcgccgt acaaccgccg tcagatcgaa gccatgtcga tgacgaagcg atagcgcaca
     3361 tccttggcct tgacgcggtc ataggcttcg ttgatgcgcg agatggggat gatgtcggca
     3421 tccgccacga tattgtgctg ggcgcagaag tccagcatct cctgggtttc cggcaagcca
     3481 ccgatcaccg agccggccac cgtcttgcgc tggatgacga gggtcgccag ataaacctcg
     3541 gtcgatttgt gatccagcgc ccctaccacc accaaggtgc cgtcgcgcct gagcaattgc
     3601 acatagggca tcaccggatg ggtacaggga atggtgctga gcagaaaatc gaacgtgcct
     3661 tggcgcttat gcatcgcgtc ttcgtcggtg gacagcagtg catccttggc gcccagacgc
     3721 gcggcatccg ccatcttggc cggtgtggtg gtgatcatcg tcacctctgc ccccatcgcg
     3781 gcggcgatct tgacgccgac atggccaagc ccgccgatgc cgacaatgcc taccttcgtg
     3841 cccggcccca ccttccaatg gcgcagcggc gaataggtgg tgatcccggc gcagagcagc
     3901 ggggcggccc cggccgcgtt catgccgtct ggaaccttca cggcaaaatg ttcggtcacg
     3961 gtgacaaggt tggaatagcc gccgtaagtg ggcgtgccca gcagcttgtc gtcgctgtta
     4021 taggtcagcg tcgcaccgtt ttcgcagaat tgttccagtc cttcgttgca gctcgcgcaa
     4081 tggccgcagg aatccaccag acagcccacc gcggccatgt cgcccttctt gaagcgcgtc
     4141 accccgtcgc ccaccttgac gacgcggccc aggatctcgt gccccgggac gcagggatat
     4201 ctggtgcccg gccattcgcc gcgcgcggta tggatgtcgg aatggcagac gccgcaatat
     4261 agaatgtcga tcagcacatc cttggggccg gtatcgcggc gcttgaaatg gaacggcgcc
     4321 agcttgccgg tggcgctggg cgcggcatag ccggtggcgg ggatggtttt cagcttgggg
     4381 gaagccgcca tggcttcggg ggacaatgcc gcaacggcgc ccagcgcggc acccgtttcg
     4441 atcagggcac ggcggctgag gccgttttcc gaagaatcgt ggttttggca atggcatttg
     4501 gctgacatgg aacatctccc tggaatttta taatttacgt cataaaacca tagagaggaa
     4561 gcactcttca attcccattc tcgtgaacgg gattaagaga gcgacattcg gcgtggcgtc
     4621 cgaggcgatg gaataaggct tcggccatgg tttccgtctt acctcctcaa gggggaggtg
     4681 tttcaactct cgtaattctc cgccaccagt cgatccagaa tccgcacgcc ccagccggtg
     4741 ccccagctcg gaatcagcgg tttgcgctca ttgtcctgcc aggcgacgcc ggcgatgtcc
     4801 aaatgcaccc aaggcgtctt gtccgtcacg aagcgctgga ggaactgcgc cgccgtgatc
     4861 gagcccgcgc tcggcccgcc gatgttcttc atatcggcga tcttggaacg cagcagcttg
     4921 tcataggctt tgccgagcgg caggcgccac accggctcgc ccacttcctt gcccgcctcc
     4981 gccaagcgct ccgacagttt gtcgtcgttg gagaaaagac cggcgtgttc cgcgcccaag
     5041 gcgaccagga tcgcgccggt cagcgtggca agatcgatca cgaatttcgg cttgaagcgg
     5101 gtctgcgcgt accacagcgc gtcggccaga accaaacggc cctctgcgtc ggtattcaac
     5161 acttcgatgg tctgcccgga catggatttc accacgtcgt cggggcgttg ggccttgccg
     5221 tcgggcatgt tttccaccag cccgatcacg cctaccgcgt tcacccgagc cttcctcgcc
     5281 gccagcgccc gcatgacacc gacaaccgcg gcggctccgc ccatgtcgcc cttcatgccc
     5341 atcatcccgg cgccggtctt gagcgacaat ccgccggaat cgaagcacac gcccttgccg
     5401 acgaaggcga cggggctgtc cttcttcttg cggccgccgt tccactgcat gacgacgagc
     5461 tggctttcgc gctcgctgcc ctggccgacg c
//
LOCUS       DASWPU010000111         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_6956, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000111 DASWPU010000000
VERSION     DASWPU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6956"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VGC27_02500"
     CDS             complement(<1..634)
                     /locus_tag="VGC27_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0281475.1"
                     /translation="MSKAGSSNWLDRPVDGEYDHILGPTDAAITLVEYGSYACPYCRA
                     ANERLAEVRDQLGDRLRYVFRHRPVTGSDLARRAAELAERADNAKRFWEIHVALMTRS
                     ETLTEDDLLAVARGLDLPPENTRKTQDAALRAKARVDADVMSARASGVIITPTFFVNG
                     RRYDGPWDEISFADAMLGRLGHRVRAAALDFASWAPSAGILLLIATALAVA"
     gene            902..2944
                     /locus_tag="VGC27_02505"
     CDS             902..2944
                     /locus_tag="VGC27_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY0281476.1"
                     /translation="MLQKHNVFRLAVATFLAILLLPAHARETLPVDQGWRFIRSDVAQ
                     GQSVSIDDSGWRKVSLPHSVNAADGDDGGNDYYRGPVWYRRHIDLKPPKEKRVFLEFG
                     AATLVADVYVNGRRAGRHEGGFSTFRFDVTALLQDGENLIAVRVDNAPDKGVAPLGGD
                     FTVFGGLIRPVKLILTGPTHIDVLDDGGPGVYVSTVDIQRDVAELAVETRIANDSDVS
                     VTSILRLHIRDAKGGVAAEAALPVTLKARETRVFRQTVRLKRPHLWDGVRDPYLYRVT
                     ADIAGDAVSVPLGVRTYRVDPAKGFILNGKPYDLHGVNYFHVQRPGRGPAVGDKEIDQ
                     DYRLMRELGSSGIRMAHFQHPARSYDNADRYGFVTWTEIALNTAYDPSPEFLANVRQQ
                     MRELAVQTFNHPAVVFLGLGNELRASDEASNRLLATAQEVAKTTDPARLTAYAHCCLA
                     DDDPLTKHADLLAYNRYWGLYQGPDTLEDVGPWADRLRAAFPDRPIGVSEYGGCASIA
                     QQEDPPSRPKPKNTWHPEQYQTKFHEAYWTQFKSRPYLWAKFVWQMFDTASDERSDDI
                     RPGINDKGLVTYDRAVKKDAFFWYKANWSKAPFVYITSRRFSPWKVPQADVKIFTNLT
                     RVTLWLNGVLVGTQNPDGRIALWRGVVLKPGDNVVIVRGRKDGASFEDRVVWRLEP"
     gene            complement(2967..3125)
                     /locus_tag="VGC27_02510"
     CDS             complement(2967..3125)
                     /locus_tag="VGC27_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281477.1"
                     /translation="MQDVNDVALFRLTLSGKRGGPSFIFDIVRAFRASGSRSLADQAF
                     AAVDRQTI"
BASE COUNT          651 a         1037 c         1050 g          646 t
ORIGIN      
        1 aggccactgc caaagccgtc gcgatcagca gcaggatgcc cgccgacggc gcccaactgg
       61 caaagtcgag cgctgccgcg cgcacacgat ggccgagtct gcccagcatc gcgtcagcaa
      121 acgagatttc gtcccagggg ccgtcgtaac ggcggccgtt gacgaagaag gtcggcgtaa
      181 tgatgactcc gctggcgcgc gcgctcatca catcggcatc gacacgcgcc ttcgcgcgca
      241 aggcggcatc ctgcgttttg cgcgtgttct ccggcggcag gtccaggccg cgggcgacgg
      301 caagcagatc gtcctcggtc agcgtttccg aacgcgtcat cagggcgaca tggatttccc
      361 agaagcgctt ggcgttgtcg gcgcgctccg ccagttcggc ggcgcggcgg gcaaggtcgc
      421 tgcctgtcac cggccgatgc cggaagacgt agcgcaggcg atcgccgagc tggtcacgaa
      481 cctcggcaag ccgctcgttc gcggcccggc aatacgggca cgcatagctg ccatattcga
      541 cgagcgtgat cgcggcatcg gtgggaccga ggatgtggtc gtactcgcca tcgaccggac
      601 ggtcgagcca attacttgat cctgccttcg acatccgtcg tcctcattcc cggcgaccgt
      661 gcgagcgatt ttctccgtcc gcgttggccg catgggcgtc gcgtaattca ggaattgtat
      721 attgaagtat cttaggggcg ccggccagcc acctatccga cgaagcgaga attttgtagc
      781 ccaggtggtc caaggttggg gcaagtccag cgccacccat ttctgaagga agacgctctc
      841 tgggaaaatt cctgttaagt tcacgtcgaa gatgcgacag tccaccaaga tgcttccata
      901 catgctgcag aaacacaatg ttttccggct ggcggtcgcc acctttcttg ccatcttgtt
      961 gctgcccgcc catgcccgtg aaaccctgcc ggtcgatcaa ggctggcgtt tcatccggtc
     1021 cgatgtcgcc cagggccaaa gcgtaagcat cgacgattcc ggctggcgga aagtctcgct
     1081 gccacacagc gtcaacgctg cagacggcga cgacggcggc aatgattact atcgcggtcc
     1141 cgtctggtac cgtcggcata tcgatctgaa gcccccgaaa gaaaagcggg tttttctcga
     1201 gttcggcgcg gcgacgctgg tcgccgacgt ttatgtcaac ggccgccgcg ccggccggca
     1261 cgagggtggt ttttccacgt ttcgcttcga tgttaccgct ctgttgcagg acggtgaaaa
     1321 cctgatcgcg gtacgggtgg acaatgcacc ggacaagggt gtggcgccgt tgggcggcga
     1381 ctttaccgtg ttcggcggct tgatccgtcc ggtcaagctg atcctgaccg ggccgacgca
     1441 tatcgacgtg ctcgacgatg gcggccccgg cgtctatgtc tcgacggtcg atatccaaag
     1501 agatgtggcg gaattggcgg tcgaaacgcg gattgccaat gattccgatg tgtccgtgac
     1561 atcaattttg cgcctgcata tccgcgatgc caagggaggc gtggcggcgg aagcggcgct
     1621 gcccgtaacg ctcaaggccc gcgagacacg ggtcttccgg caaaccgttc gtctgaagcg
     1681 gccgcatctg tgggacggcg tgcgcgatcc ctatctttat cgtgtgacgg cggacatcgc
     1741 cggggatgcg gtatcggtgc cgctcggcgt gcgcacctat cgcgtcgatc ccgccaaggg
     1801 gttcatcctc aatggcaagc cttacgatct gcacggggtg aattatttcc acgttcagcg
     1861 gccgggcagg gggccggcag tcggcgacaa ggaaatcgat caggattacc gcttgatgcg
     1921 cgaactgggg agcagtggta tccgtatggc gcatttccag catccggcac ggtcttacga
     1981 caacgccgac agatacgggt tcgtcacctg gaccgagatt gcgctcaaca cggcttacga
     2041 tccctcgccg gaatttctcg ccaatgtccg ccagcagatg cgcgaattgg ccgtgcaaac
     2101 cttcaaccat cccgccgtgg tgttcctggg gctgggcaac gagttgcgtg cctccgatga
     2161 agcctccaac cgcctcctgg caacggcgca ggaggttgcc aagacgaccg acccggcacg
     2221 tttgaccgcc tatgcccatt gctgcctggc ggatgacgat cctttgacga aacacgccga
     2281 tcttctggcg tacaatcgct attggggtct ctaccaaggc cctgacacgc tggaggatgt
     2341 cggcccttgg gccgaccgcc tgcgcgccgc cttccccgac cgccccatcg gcgtcagcga
     2401 atatggcggc tgcgcctcga tcgcgcagca ggaagatccg ccctcgcggc ccaagccgaa
     2461 aaacacctgg catcccgagc agtatcagac caaatttcac gaagcctatt ggacccagtt
     2521 caagagccgg ccctatctat gggcaaaatt cgtctggcag atgttcgata ccgcttccga
     2581 cgaacgcagc gacgacatcc gtcccggcat caacgacaag ggactggtca cctacgaccg
     2641 tgccgtgaag aaggatgcct tcttctggta caaggccaat tggtcgaagg cgcccttcgt
     2701 ttacatcacc agccgccgct tcagtccgtg gaaagtgccg caggccgatg tcaaaatatt
     2761 caccaatctg acccgggtga cgttgtggct gaacggggtg ctggtcggta cgcagaaccc
     2821 ggacgggcgc attgcgctgt ggcgcggtgt cgtgctgaaa cccggcgaca acgtcgtgat
     2881 cgtgcgaggg cgcaaggacg gcgcgagctt cgaggatcgc gtcgtctggc ggctggagcc
     2941 atgagggaaa actcgcggaa ccgcgctcag atcgtctggc gatcgacggc ggcaaacgcc
     3001 tgatcggcga ggctgcggct gccggaagca cggaacgccc tgacaatgtc gaatatgaat
     3061 gacgggccac cccgcttgcc cgacagggtg aggcggaaga gagcgacgtc gttgacgtcc
     3121 tgcatcctga acaccggcct tgcatcggca ttctcgcatt ctatttcgat gtggttgaat
     3181 tctgcgttgc gcacttggcg catgaaaagc cggctacgcc tgcgcggata gaggcagcgg
     3241 agcaggtcaa caacgcagtt ccctagtcga agcagatcat gagcgatgcg attctcgaca
     3301 tcggattgtc ccccaacagg ccgatccgtt tactgacgga acgtcgtttg gcgcaagaat
     3361 agttacgccg cgcaatcgcg gctc
//
LOCUS       DASWPU010000112         7330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_86328,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000112 DASWPU010000000
VERSION     DASWPU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86328"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VGC27_02515"
     CDS             <1..567
                     /locus_tag="VGC27_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281478.1"
                     /translation="LTPQTANSNAPEVSSACSRIGVTSCSGSYAGNINRAQYAMRVGG
                     YTPQQLAEVPVVMSAINQAWYDSNPNAVRRNLKREAHFYVLQDKATGKISVSRITLSS
                     LGAAEWTPPPPPRLATAIVLADFHTHPWLESDFGFPVRIPHGPSGGEGNFPGDIDALR
                     YKRIPLGNIRDHTGLVFYDQNGVIGNGH"
     gene            577..882
                     /locus_tag="VGC27_02520"
     CDS             577..882
                     /locus_tag="VGC27_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281479.1"
                     /translation="MRITATVFALCLVIPLSNGCAIAQQSGASEVSESTLLKIASDAI
                     ETKIWKGATPEAKYAVIVDQGDKWEIDYRYPPPRRPVVGRVVIDKKTMQVTRIDFNE"
     gene            complement(1222..2187)
                     /locus_tag="VGC27_02525"
     CDS             complement(1222..2187)
                     /locus_tag="VGC27_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007635327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEY0281480.1"
                     /translation="MGQVLHVCATTTEAVRRAIQNSQASLRALSKHYGINQKTVAKWK
                     KRGFVADLPTGPREPKSTVLSVEDEAIIVAFRRHTLLPLDDCLYALQPTIPHLTRSSL
                     HRCLQRHGISRLPEVNGDKPDKKKFKIYPIGYFHIDIAEVRTAEGKLYLYVAIDRTSK
                     FAFVQLVKTTGRTTASAFLVALIKAVPYKIHTVLTDNGIQFTFPPRYADGPTARYMTH
                     MFDMRCRENGIEHRLTKIKHPWTNGQVERMNRTIKEATVKRYHYDSHRQLQSHLTDFI
                     NAYNFGRRLKTLKGLTPYEFICKIWTKEPKRFRIDPIHQLPGLNT"
     gene            complement(2261..2572)
                     /locus_tag="VGC27_02530"
     CDS             complement(2261..2572)
                     /locus_tag="VGC27_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281481.1"
                     /translation="MKLVHTVIWALLASAIIAIPALAFLRRFDWIAVITGLVVLEGIA
                     LRANGGRCPLRSLAAKYTDDRRDGFDIYLPGWLARNTMPVFGTWFVAGELLALWRWFL
                     G"
     gene            complement(2635..3855)
                     /locus_tag="VGC27_02535"
     CDS             complement(2635..3855)
                     /locus_tag="VGC27_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0281482.1"
                     /translation="MRALASVPAFRNDHTEWEAGALANPLPAFDASTSCAEVYERFNG
                     PEPQIAAAVVDGENRVLGIVNRLRFLARYAQRYVPELYGKQPIIKLANCKALVVDEHM
                     PVADLGAMITLDWPDALRESFVVTRAERYLGIGTSEALVRSKVALLMDREEQLHAALI
                     AAHDANQTKGHFLALMSHELRTPLNAIIGFSEVLASELFGPHGNARYCEYARDIHGAG
                     RHLLALINDILDLSKSEAGKLELYPEPVQLPQLFDDCVRLVAERARKQGLTITQSVPR
                     DFPCLDADALRLKQILLNLLSNAVKFTLAGGSVTVEAACAGDGGIVIRVSDTGIGMAE
                     ESIPIALEPFRQIASPMSRNVEGTGLGLSLVRTLTEQHGGRLEIQSTPSVGTTVELCF
                     PPGRTVRENLDKSA"
     gene            complement(3984..4619)
                     /gene="queE"
                     /locus_tag="VGC27_02540"
     CDS             complement(3984..4619)
                     /gene="queE"
                     /locus_tag="VGC27_02540"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEY0281483.1"
                     /translation="MTYQVKEIYYTLQGEGARSGRASVFLRFAGCNLWSGQERDRANA
                     VCKFCDTDFAGTDGEGGGKFASAEVLARATAGCWPKDAVAQPYVVCTGGEPLLQLDDA
                     LIAALHAQGFEIGIETNGTLAAPHGIEWICVSPKADAELVQTSGQELKLVYPQHGAPP
                     ERYAALIFDYFYLQPMDGPAREANTRAATAYCLAHPQWRLSLQTHKLIGIR"
     gene            complement(4616..5314)
                     /gene="queC"
                     /locus_tag="VGC27_02545"
     CDS             complement(4616..5314)
                     /gene="queC"
                     /locus_tag="VGC27_02545"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY0281484.1"
                     /translation="MNTDTALVLFSGGQDSTTCLAWALARFGRVETVGFDYGQRHRTE
                     LIARERVLGAVRARFPAWAEKLGDDHLLSLDLLKQIGGTALTDATKIEMGADGLPTTF
                     VPGRNVMFLTAAAALGYRRGIADLVGGMCETDFSGYPDCRDKTIAATAEALTLALARR
                     VSVHTPLMWIDKAATWALAEELGGASLVDLIREETVTCYEGDRGHRHDWGYGCGVCPA
                     CELRAKGYAKYMAR"
     gene            complement(5343..6716)
                     /locus_tag="VGC27_02550"
     CDS             complement(5343..6716)
                     /locus_tag="VGC27_02550"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY0281485.1"
                     /translation="MVSTRLKRLGSAGSRRAGPEASRLSPSGILVSLLLGGVIGVAST
                     TIDQGEFAAGLVIMVVLGIAVLWLRRVAERTDKRLIAGDVSYRAFFENAIEGIFRTTP
                     DGHYLDVNPALAEIYGYPSPEALMNGLTDIASQLYINPGRREEFKTLMQMNDRVAGFV
                     SEIRRRDGSTIWISENARAVRDWTGRLVCYEGTVENVTAKFEAERVMRQGLREAEEAN
                     RAKSAFLAAMSHELKTPLNAIIGFSEIMKEEAFGPIGQDSYRGYAADIHASGTKLLSI
                     INDVLDVSRLEGGAITIEPQPCRVRDLAGDALEIARRLTGDGREVSIDMAQTLPAIHA
                     DPGRLRQVLVNLLTNALKFTPEGGAVSVRAWLEKAGGVSIAVIDGGIGMAPEKIAAAL
                     EPFRQLDGSLARRFEGAGLGLSIAKALVELHGGRLSVKSSVGRGTAVTVAMPAARTRR
                     RDIPAVA"
     gene            6845..>7330
                     /locus_tag="VGC27_02555"
     CDS             6845..>7330
                     /locus_tag="VGC27_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY0281486.1"
                     /translation="MTGSVVSGTGLCTPAQTISNDELVASFNEYVRRTNATHAEAIAA
                     GSVTALQPSSAEFIVKASGVKNRHVIDKAGLLDPKIMCPRIPERSNGQLSVSAEMAAA
                     AGRDALVHARREARDIDAVIVSCANLERPYPAIAIEVQDALGIEGFGFDMNVGCSSAA
                     FA"
BASE COUNT         1423 a         2266 c         2224 g         1417 t
ORIGIN      
        1 ctcacaccac aaacggcgaa ctcaaatgca ccggaggtta gtagtgcttg ttcgagaatt
       61 ggggtcacaa gttgcagcgg ctcttatgca ggtaacataa atagagcaca gtatgcgatg
      121 cgggtgggtg ggtacacacc gcagcagctt gcagaggttc ctgttgtgat gtcggcgatc
      181 aatcaggctt ggtatgactc aaacccaaat gcggtgcggc ggaatttgaa gcgtgaggct
      241 cacttctatg tgctgcagga taaggcgacc ggaaaaatct ctgtgagccg tatcacgtta
      301 agttcgctcg gcgcagcaga atggacgcca cctccacccc cacgattggc gaccgcaatc
      361 gtattggcag attttcacac gcatccgtgg ctagaatccg atttcggctt tcctgttcgg
      421 attccacacg gaccgagcgg tggtgaaggg aactttccgg gtgacattga cgcgctgaga
      481 tacaaacgca tccctctcgg caatatcagg gaccacactg gcttggtttt ttacgaccag
      541 aacggcgtca ttggaaacgg acactaggag gaaaagatga ggattactgc aacggtgttt
      601 gcactttgtc tggtgattcc actgtccaat ggctgcgcaa ttgcccaaca atccggagcg
      661 agtgaagtga gtgagtcaac actactcaag atcgcttctg atgccatcga aacgaaaatc
      721 tggaagggcg cgacgccgga ggcaaagtac gcggtgatag tcgatcaggg ggataagtgg
      781 gagatcgatt acaggtatcc ccctcctaga agaccagtgg ttggacgtgt agtcatcgac
      841 aagaaaacca tgcaggtaac ccggatcgac ttcaacgagt aagcattttg tgtctggccc
      901 cctttgtgga gtgcgatctg cggattcaac gtgtgtcgac gtgaaatccc ccatcttgcg
      961 tgttcggttc caccgcctga attgcattag gatcgatgat ccggcggggt gtttttgatc
     1021 gcggatttgc gtcgtgcacg gtgatgttgg cgctgtcccg atgctccgat tttgcatcgt
     1081 cagcacggcg tcggtagcag ttccggagag cgttgcgcgg ccggtttgcc attggaacag
     1141 atggtgatcg gcggactcgc gaaaaatcgc tgatagggcg cgagaaaacg caacctcttc
     1201 ctcaaccgat ctgcagggac cctaggtgtt tagtcccggc aattggtgga tcggatcgat
     1261 tctgaatcgt ttcggttctt ttgtccagat tttgcagatg aactcgtacg gcgtgagacc
     1321 tttcagggtc ttgagccgcc tgccgaagtt gtaggcattg atgaagtcgg tgaggtggct
     1381 ctggagctgc cggtggctgt cgtagtgata acgcttgacg gtggcttcct tgatcgtccg
     1441 gttcatgcgc tcgacctgac cgttcgtcca tggatgctta atcttggtga gcctgtgctc
     1501 gataccgttc tcgcggcagc gcatgtcaaa catatgggtc atgtacctgg ccgtcggacc
     1561 gtcggcgtaa cgcggtggaa aggtgaattg gatgccgttg tcggtgagta cggtgtggat
     1621 tttgtacgga acggctttaa tcagtgccac caggaaggcg gatgccgtgg ttctgcctgt
     1681 ggtcttcacc agctgcacga aggcgaattt gctcgtccgg tcgatcgcaa catacaggta
     1741 cagcttgcct tcggcggtgc ggacctcggc aatatcgatg tggaaatagc cgatcggata
     1801 gatcttgaac ttcttcttgt ccggcttgtc gccattgacc tccggaagcc gcgaaatgcc
     1861 atgacgctgc aggcagcgat gaagcgagga gcgcgtcagg tgcgggattg ttggctgcag
     1921 cgcatagagg cagtcatcca gcggcagcag cgtgtggcgg cggaaggcaa cgatgatggc
     1981 ctcgtcttcg accgacagca ccgtggactt cggctctctc gggcccgtcg gcagatcggc
     2041 aacgaagcca cgcttcttcc atttggccac ggtcttctgg ttgatgccgt agtgtttgga
     2101 aagcgccctc aggctcgctt gactattttg tattgctcga cggaccgcct ctgtcgtcgt
     2161 ggcgcagacg tgtaaaactt gtcccatagt gcctccttcc atccaaagga taagaatgca
     2221 ccatcaaaat ccgggactaa acacctagac cgcccgcgcc tcaacccaga aaccagcgcc
     2281 acagcgccag cagctcgccc gcgacgaacc aagtgccgaa aaccggcatc gtgttccgcg
     2341 ccagccagcc gggcagatag atgtcgaaac cgtcccggcg gtcgtccgtg tatttcgcgg
     2401 ccaagcttcg cagcggacat ctgccgccgt tcgcccggag tgcgatcccc tccagcacaa
     2461 ccaatcccgt aatgacggcg atccaatcga agcggcgcag gaacgccaga gcgggaatcg
     2521 cgattatggc cgacgcgaga agcgcccaga tgaccgtgtg aacaagtttc accgctacca
     2581 gcattcgcgt tcggccggac atggaaaaga tcgtagcgcg gttccggccc ggcgtcaggc
     2641 gctcttatcc aaattctcgc ggaccgtgcg gccgggagga aagcacagtt ccaccgtcgt
     2701 gcctacgctc ggggtgcttt ggatctcgag cctgccgcca tgctgttcgg tcaaggtgcg
     2761 gaccagggac aggcctagcc cggtgccctc gacattgcgc gacattggag aggcaatctg
     2821 ccggaacggc tcaagtgcga tcgggatgga ttcctccgcc atgccgatgc ccgtatcgct
     2881 gacgcggatc acgatcccac cgtctcccgc gcaagcggcc tcaacggtca cgcttccccc
     2941 ggcaagcgtg aacttcaccg cattcgacag aaggttcagc agaatctgct tcaggcgcaa
     3001 ggcgtcggcg tccagacaag ggaaatcccg cggtaccgat tgcgtgatgg tcaggccctg
     3061 cttgcgggcg cgctccgcca ccaggcgcac acaatcgtcg aaaagctgcg gcagctgaac
     3121 cggctccgga taaagttcga gttttccggc ttcggatttc gacaaatcga ggatgtcgtt
     3181 gatcaacgcc agaaggtgcc tgccggcgcc atggatgtcg cgggcgtatt cgcagtagcg
     3241 cgcattgccg tgcgggccga acaactcgct cgccagcacc tccgaaaagc cgatgatggc
     3301 gttgaggggc gtccgcagtt catggctcat caaggccagg aaatggcctt tggtctgatt
     3361 ggcgtcgtgg gccgcgataa gcgccgcatg aagttgctcc tcccggtcca tcagcagcgc
     3421 gaccttggaa cgaaccagcg cctcgctcgt accgatgccc aaatagcgtt ccgcgcgcgt
     3481 caccacgaaa ctttcgcgca gggcgtccgg ccagtccagc gtgatcatcg cgcccagatc
     3541 cgccaccggc atgtgctcgt cgacgaccag ggccttgcag ttggcaagct tgatgatcgg
     3601 ctgcttgccg taaagttccg gaacatagcg ttgcgcgtag cgcgccagga agcgcagccg
     3661 gttgacgatg ccgagaacgc gattttcgcc gtccacgacc gcggcggcga tctgcggctc
     3721 gggcccgttg aaccgctcat acacttccgc acaagatgtc gaagcatcga aggccggaag
     3781 cggatttgcc aacgcgcccg cctcccattc cgtgtggtcg ttccgaaacg ccggaacgga
     3841 agcaagcgca cgcataaaca agtccccgcc aaaacttgcc tgaagaataa gggcaccccc
     3901 ttgtgcctcc gttaacggcc cgcgcggggt aatatgaaat tatcgtgacg ttgttggcgc
     3961 ggccaacatc gagggcggcg cggtcagcgg ataccgatca gcttgtgcgt ttgcaggctg
     4021 aggcgccatt gcggatgggc gagacaatag gccgtggcgg cgcgtgtatt ggcttcgcgc
     4081 gcgggcccgt ccatcggctg caaatagaaa tagtcgaaga tgagtgcggc gtaacgctcc
     4141 ggcggcgcgc cgtgctgcgg gtagaccagc ttcaactcct gaccgctggt ctgcaccaat
     4201 tccgcatcgg ccttggggct gacgcaaatc cattcgatgc cgtgcggcgc ggccaaggtg
     4261 ccgttggtct cgatgccgat ctcgaagcct tgcgcgtgca gcgccgcgat cagggcatcg
     4321 tcgagttgca gcaaaggctc tccgccggtg cagacgacgt aaggctgcgc caccgcgtcc
     4381 ttgggccagc atccggctgt cgcccgcgcc aaaacttcgg cgctggcgaa cttgccgccg
     4441 ccctccccgt ccgtgcccgc gaaatccgta tcgcagaatt tgcacacggc attcgcccgg
     4501 tcgcgctcct gtccgctcca cagattgcag ccggcgaagc gcaggaacac gctcgcgcgg
     4561 ccgctgcgcg cgccctcgcc ttgcagcgtg taatagattt ctttcacctg ataggtcatc
     4621 gcgccatgta cttcgcgtag cctttggcgc gcagctcgca ggccgggcag acgccgcagc
     4681 catagcccca atcgtgccga tggccgcggt cgccttcgta gcaggtgacg gtttcctcgc
     4741 ggatcaaatc gaccagcgac gctccgccta gttcttcggc cagcgcccaa gtcgccgcct
     4801 tgtcgatcca catcaagggc gtgtgaacgc tcacgcgccg cgccagcgcc agggtcagcg
     4861 cctcggccgt ggccgcgatg gtcttgtcgc ggcagtcggg atagccggaa aaatccgtct
     4921 cgcacatgcc gccgaccaga tcggcaatgc cgcggcgata gcccagcgcg gcggcggcgg
     4981 tcaggaacat cacgttgcgc cccggcacga aagtggtggg cagtccgtcc gcgcccattt
     5041 cgatcttggt cgcatcggtg agcgccgtac cgccgatctg cttcagcagg tcgagtgaaa
     5101 gcagatggtc gtcgccgagc ttttccgccc aagcgggaaa ccgcgcccgg accgccccca
     5161 gcacgcgctc gcgtgcaatc aactcggtgc ggtgcctttg gccgtaatcg aagcccacgg
     5221 tctcgacccg cccgaaacgc gccagcgccc aggccagaca ggtggtcgaa tcctgtccgc
     5281 cggaaaagag caccaaggcg gtgtccgtgt tcatggggga tgatttagcg cgttggcgcg
     5341 ctctaggcca ccgccggaat atcgcgcctg cgcgtgcggg ccgcgggcat ggcgacggtc
     5401 accgccgtac cccgccccac cgagcttttg acggacagcc tgccgccatg cagttccacc
     5461 agcgccttgg cgatggaaag gcccaggccc gcaccctcga aacgccgcgc cagcgaaccg
     5521 tccaactggc ggaaaggttc gagcgcggcg gcgatcttct ccggcgccat gccgatgccg
     5581 ccgtcgatca ccgcgattga gaccccgccc gccttttcca gccaggcgcg gaccgacacc
     5641 gcgccgccct caggcgtgaa tttcagagca ttggtcagga gattgacaag aacctggcgc
     5701 aaacggccgg ggtcggcatg gatggcgggc aaagtctgcg ccatgtcgat gctcacctcg
     5761 cgcccgtcgc cggtcagccg ccgcgcgatc tcaagcgcgt ctccggccag atcgcgcacg
     5821 cggcaaggct gcggctcgat ggtgatcgcc ccgccctcca atcgcgaaac atccagcacg
     5881 tcgttgatga tcgacaacag cttggtgccg ctggcgtgga tgtcggcggc ataaccgcga
     5941 taggagtcct ggccgatggg gccgaacgct tcttccttca tgatctcgga aaagccgatg
     6001 atcgcgttca acggcgtctt caattcgtga ctcatcgccg cgaggaaggc gctcttggcg
     6061 cggttcgcct cttcggcctc gcgcaatccc tgccgcatca cgcgctcggc ctcgaacttc
     6121 gccgtgacgt tctccaccgt gccttcgtag cagaccagcc ggccggtcca gtcgcgcacc
     6181 gcgcgcgcgt tctcggaaat ccagatggtc gaaccgtccc gtctgcggat ttcggaaacg
     6241 aaacccgcca cccggtcgtt catctgcatc agggtcttga actcttcgcg gcgcccggga
     6301 tttatataga gctgggaagc gatgtcggtc agcccgttca tcagcgcctc cggcgacgga
     6361 tagccgtaga tttccgccaa ggcaggattg acgtccagat aatgcccgtc gggcgtggtg
     6421 cggaaaatgc cctcgatcgc attctcgaag aaggcgcggt agctcacgtc gcccgcgatc
     6481 aggcgcttgt cggtgcgttc ggcgacgcgc cggagccaca gaaccgcaat ccccaataca
     6541 accatgatga caaggccggc ggcaaactcg ccttgatcga ttgtggtgga agcgacgccg
     6601 atcacgccgc ccagcagaag cgataccagt atgccggacg gcgaaagacg gctcgcctcc
     6661 gggccagccc gcctgctccc ggccgaaccg agccgcttta atcgcgtaga aaccacgttc
     6721 cccaccgaga cgcaaaatac tcggcactaa tcctaacgga caaacctaaa tgttgggttg
     6781 aggcgtgctt gccttctatt tgtgcatcgg tcacgctgtt ctgcataatc aagaggatgc
     6841 tgtggtgacc ggatcagttg tttcggggac ggggctttgc acgccggcgc aaacgatctc
     6901 caatgacgaa ctcgtcgcct ccttcaacga atatgtccgc cgcaccaatg caacccatgc
     6961 cgaggcgatt gccgccggtt cggtgaccgc tttgcagcca tcctcggccg aattcatcgt
     7021 caaggcttcg ggcgtcaaga accgccacgt catcgacaag gccgggctgc tcgatcccaa
     7081 gatcatgtgt ccccgcatcc cggagcggtc gaacggccag ctttccgtct cggcggaaat
     7141 ggcggcggcg gcggggcgcg acgcgctcgt ccatgcccgc cgcgaagcca gagacatcga
     7201 cgccgttatc gtcagctgcg ccaatctgga gcggccttat ccggccatcg ccatcgaggt
     7261 ccaagacgcg ctcggcatcg agggcttcgg cttcgacatg aatgtcggct gctcgtccgc
     7321 cgccttcgcc
//
LOCUS       DASWPU010000113         8825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_6976, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000113 DASWPU010000000
VERSION     DASWPU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8825
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6976"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            613..4407
                     /locus_tag="VGC27_02560"
     CDS             613..4407
                     /locus_tag="VGC27_02560"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005736.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEY0281487.1"
                     /translation="MRIRRQFTVEGRSPFDGIAFRQGTSEIRNPDGSVVFRQTDIEVP
                     EDWSQVACDVLAQKYFRKAGVPASLAPVPEDGVPEWLWRKTADSSETVGESSARQVFT
                     RMAGTWTYWGWKGGYFDNEADARAFHDELCHMLATQKAAPNSPQWFNTGLYWAYGIDG
                     PSQGHYYVDPFSRRLTKSNSAYEHPQPHACFIQSVADDLVNEGGIMDLWTREARLFKY
                     GSGTGTNFSALRGENERLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVIVDI
                     DHPDIEDFINWKVIEEQKVAAMVAGSRLAEKHLKAVLKACVNCQGSGDDCFDPRKNPA
                     LRREVKAARKSMIPDNLIERVISFAKQGYQDIDFRTYDTDWDSEAYRSVSGQNSNNTV
                     RVTDEFLHAVLDDGDWHLTARKGGKIVKTMKARELWDKISYAAWACADPGIQFHTTIN
                     DWHTCPEGGEIHASNPCSEYMFLDDTACNLASLNLMAFRKDEDGKPASSSEAIGQKKI
                     FDVAAFQHAVRLWTIVLEISVLMAQFPSKEIAQRSYEYRTLGLGFANIGGLLMAAGIP
                     YDSREARAIAGAVSSVMTGTSYAASAEMASELGAFPEFAANREHMLRVIRNHRRAAYG
                     ESKTYEGLSALPVPLDLSAIKQTDLARAARTAWDDALVVGEEFGYRNAQATVIAPTGT
                     IGLVMDCDTTGVEPDFALVKFKKLAGGGYFKIINRCVPEALATLGYDQKQIDDIVAYA
                     VGHGTLDGFSGALTNEALRAKGFGEEELGKIEAALESAFDIRFVFNKWTLGETFCVET
                     LKLDPEAMDAADFDMLKALDFAKADVDTANLFVCGAMTLEGAPHLKPEHLPVFDCANP
                     CGRIGTRSLSVESHIRMMAAVQPFISGAISKTINMPNKATVKECGESYLLSWKLGLKA
                     NALYRDGSKLSQPLASQIFAFDDDEDELEETLAAPAAARTSVVAERVVEKIIERVSRE
                     REKLPQRRKGYTQKAIVGNHKVYLRTGEYEDGRPGEIFIDMHKEGAAFRSMMNNFAIA
                     ISIGLQYGVPLEEFVDAFTFTRFEPAGIVIGNDSIKNATSVLDYIFRELAVSYLGRND
                     LAHVPPHHNEDLGGGVAEGQRAAKGEMQTVNRTTSVGYVRGNFHRIAVVRGGAAAKMM
                     ELPEPERENDLGNGRSHDHDDVREFLEAGSVDLSEIASSVEATLASAGVSTANPVGVQ
                     MAAIETRLAASAQDVRAQRAAEARMKGYDGDACGACGNFTLVRNGTCMKCDTCGGTSG
                     CS"
     gene            complement(4575..4862)
                     /locus_tag="VGC27_02565"
     CDS             complement(4575..4862)
                     /locus_tag="VGC27_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY0281488.1"
                     /translation="MRRGFVYIMASKRNGTLYLGVTSNLLERVTQHREGHGSDFVKRY
                     KVTMLVWYEEWALITDAIQRETSLKRWSRKWKLALIEKMNPDWRDLYGDLF"
     gene            5069..5533
                     /locus_tag="VGC27_02570"
     CDS             5069..5533
                     /locus_tag="VGC27_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0281489.1"
                     /translation="MIRTALAALALAACAATAAYGQDKQTAYLDAVASIKAGNRDCPN
                     CVLAGADLTNQCVKGGNLTGADFDHARLVLMCMSYSDFRNATFRGADLSGANLAHAKL
                     DGADFTGARLTITSIKGADLSHAKGLTQSQLDAACGDAETKAPAGLQVKTCS"
     gene            complement(5655..7169)
                     /gene="gcvPB"
                     /locus_tag="VGC27_02575"
     CDS             complement(5655..7169)
                     /gene="gcvPB"
                     /locus_tag="VGC27_02575"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEY0281490.1"
                     /translation="MTVATISGDRGLDHEEKLIFELGRAGMCGVDLPQVSVGDSRLAG
                     QRRKGPIGLPGLSEPEVVRHYVRLSRMNYAIDAGLYPLGSCTMKHNPRLGEKLARLPG
                     FADLHPLAPQSAAQGALALIAELMRWLTTLTGMPAVAMSPKAGAHGELCGMLTIRAAL
                     LARGEKRPRVLVPESAHGTNPATAAFAGFTVDEVKATPDGRVDVEALKAKLGPDVGAL
                     MLTNPNTCGLFERDVVEIAGLVHKAGAYFYCDGANFNAIAGRVRPGDLGVDAMHINLH
                     KTFSTPHGGGGPGAGPVVLSAALAPFAPLPLLVQEVNGFRLIEDRADAPAEAQPFGRM
                     CAFHGQMGMFVRALAYMLSHGRGGVRQASEDAVLSANYILASLKDVMSAPYEGPCMHE
                     ALFDDRFLKDTGVSTLDFAKAMIDEGFHPMTVYFPLVVHGAMLIEPTESESKESLDRF
                     IHVLRTLALEAKGGQRERFDGAPKFTPRRRLDETAASRKPVLRWKPKGLPHAAE"
     gene            complement(7166..8512)
                     /gene="gcvPA"
                     /locus_tag="VGC27_02580"
     CDS             complement(7166..8512)
                     /gene="gcvPA"
                     /locus_tag="VGC27_02580"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEY0281491.1"
                     /translation="MRYLPLTPSDRAAMLAKIGVPDIDALFRDVPKSAVVPLSAFDLP
                     DTQGELEVERALSCLASRNVAAEAGPFFCGAGAYKHHVPAAVDHLIQRSEFLTSYTPY
                     QPEISQGTLQYLFEFQTQVALLTGMEVANASLYDGSTATAEAVLMAQRLTRRSKAVLS
                     GGLHPHYAETVETVANLSGKVVRAPVTPGRAEDIAALIDEDTACVVVQSPDVFGLIRD
                     LQPVADAAHAKGALLIAVVTEIVSLGLIEPPGTQGADIVAAEGQSIGNGLNFGGPYVG
                     LFATREKFIRQMPGRLSGETVDAEGRRGYVLTLSTREQHIRREKATSNICTNSGLCAL
                     AFTIHLSLLGEEGFAQLARLNHAKASALADRLVKVKGVSLVTPSFFNEFTLRLPRPAA
                     EIVEALAAKDILAGVPASRLLPHEAAARDLLIVAATETNTGEDFAAFAEALAEVLS"
     gene            complement(8580..>8825)
                     /locus_tag="VGC27_02585"
     CDS             complement(8580..>8825)
                     /locus_tag="VGC27_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEY0281492.1"
                     /translation="GRDVAKGAEAAVVESVKAASEIYALVGGAVTAVNEALNADPAKV
                     NADPEGEGWFFKLKLADAAELSTLMTRAQYDEYVKGL"
BASE COUNT         1647 a         2918 c         2778 g         1482 t
ORIGIN      
        1 gtgaagcagg aaggacgagc aggaagtacg aggtcggagt gagctagccc gaaaaggggg
       61 tgttgctcct atccccctat tcccccattc cgtcccccga acactcgcgg ctggatggcc
      121 gggtcaagcc cggccatgac gaaagtgggg ttacgatcaa gggcctcggt ttacccatcg
      181 atttcgcctc catccacagc ccgtcttcgc ccttcgggct acgacgctgc aagcgcgggg
      241 gcagaaactc ctccccatcc gcttcgatcc acagcccgtc cacaaggtcg cgcgggcctg
      301 tgcgtcataa aaacgaaaaa aatttctgcg cgattctgaa accccgcttg actcgcggct
      361 tttgtgcacc gcgttaagac actacatctg gtgggtaagg tttcgtttac cacttatcgc
      421 ttgcgtgccc cccgcgatgg ggccaatact ttggctcagt cgcttctcgc gacaccaaat
      481 ctggcggatc gagcaccctc ttgccgccgg ggttcctgcc gcacaggcaa aactccgcgg
      541 agaggaaaac tgtccccaaa acggtgccga aagagcggtt gcgggcccgc tttctgtttg
      601 ctgagggata acatgcgtat tcggcgtcaa ttcacggtgg aaggccgctc gccctttgat
      661 ggaatcgcct tccgtcaggg caccagcgag atcagaaacc cggacggttc ggtcgtcttc
      721 cggcagaccg acatcgaagt ccccgaggat tggagccaag tcgcttgcga cgttctggcc
      781 caaaaatact tccgcaaggc cggcgtgccc gcctcgctgg cgcccgtccc cgaagacggc
      841 gtgcccgaat ggctgtggcg caagacggcc gacagcagcg agacggtggg ggagtcttcc
      901 gcccggcagg tgttcacccg catggcgggc acctggacct attggggctg gaaaggcggc
      961 tatttcgaca acgaagccga tgcgcgcgcc ttccatgacg agctttgcca catgctggcg
     1021 acgcaaaagg cggcgccgaa ctcgccgcaa tggttcaata ccggcctcta ctgggcctat
     1081 ggcatcgacg gaccgagcca gggccattac tacgtcgatc ccttcagccg ccggctgacc
     1141 aaatccaact ccgcctacga gcatccccag ccgcacgcct gcttcatcca gtccgtcgcc
     1201 gacgatctcg tcaacgaagg cgggatcatg gacctgtgga cgcgcgaggc gcgcctgttc
     1261 aaatacggct cgggcaccgg caccaacttc tcggcgctgc gcggcgagaa cgaaaggctc
     1321 tccggcggcg gcaaatcctc cggcctgatg agcttcctca agatcggcga ccgcgcggcg
     1381 ggcgccatca agtcgggcgg caccacgcgc cgcgccgcca agatggtgat cgtcgatatc
     1441 gaccatcccg atatcgagga tttcatcaat tggaaggtga tcgaggaaca gaaggtcgcc
     1501 gccatggtgg ccggctccag gctggccgaa aagcacctga aagcggtcct gaaagcctgc
     1561 gtcaattgcc aaggctcggg cgacgactgc ttcgatccgc gaaagaaccc ggcgctgcgc
     1621 cgcgaagtga aggcggcgcg caagtcgatg atccccgaca atctgatcga gcgggtgatc
     1681 tccttcgcca agcagggcta ccaggacatc gacttccgca cctacgacac cgactgggat
     1741 tcggaagcct atcgctcggt ttcgggccag aactccaaca acaccgtgcg ggtgaccgac
     1801 gagttcctgc acgccgtgct ggacgacggc gactggcatc tgacggcgcg caagggcggc
     1861 aagatcgtca agaccatgaa ggcgcgcgaa ttgtgggaca agatctccta cgccgcctgg
     1921 gcctgcgccg atcccggcat ccagttccac accaccatca acgactggca cacctgcccg
     1981 gaaggcggcg aaatccacgc ctccaacccg tgctcggaat acatgttcct ggacgacacg
     2041 gcctgcaatc tcgcctcgct gaacctgatg gccttccgca aggacgaaga cggcaagccg
     2101 gcctcaagta gcgaagcgat aggccaaaaa aagattttcg acgtcgccgc cttccagcac
     2161 gcggtgcggc tgtggaccat cgtgctcgaa atctcggtgc tgatggcgca gttcccgtcg
     2221 aaggaaatcg cgcagcggtc ctacgaatac cgcaccctgg ggctgggctt cgccaatatc
     2281 ggcggcctct tgatggcggc cggcattccc tacgacagcc gcgaagcgcg cgccatcgcc
     2341 ggcgccgttt cctcggtgat gaccggcacg tcctatgccg ccagcgcgga gatggcgagc
     2401 gaactcggcg ccttccccga attcgccgcc aaccgcgaac atatgctgcg cgtgatccgc
     2461 aaccaccgcc gcgcggccta tggcgaaagc aaaacctatg aaggcctgtc cgccctgccc
     2521 gttccgctcg acctctccgc catcaagcag accgatctgg cgcgcgcggc caggacggcc
     2581 tgggacgacg cgctggtggt cggcgaagag ttcggctacc gcaacgccca ggcgacggtg
     2641 atcgccccca ccggaacgat cggcctggtg atggactgcg acaccaccgg cgtcgagccg
     2701 gacttcgcgc tggtgaagtt caagaagctg gccggcggcg gctatttcaa gatcatcaac
     2761 cgctgcgtgc ccgaagcgct cgccaccctg ggctacgacc agaagcagat cgacgacatc
     2821 gtcgcctatg cggtcggcca cggcacgctg gacggcttta gcggggcgct gaccaacgag
     2881 gcgctgcgcg ccaagggttt cggcgaggaa gaactcggca agatcgaagc ggcgctggaa
     2941 tcggccttcg acatccgttt cgtcttcaac aaatggacgc tgggcgagac cttctgcgtc
     3001 gaaacgctga agctcgaccc ggaggcgatg gacgccgccg atttcgacat gctgaaggcg
     3061 ctggacttcg ccaaggccga tgtcgatacg gcgaacctgt tcgtctgcgg ggcgatgacg
     3121 ctggaaggcg cgccgcacct gaagccggaa catctgccgg tgttcgactg cgccaatccg
     3181 tgcggccgca tcggcacccg ttcgctctcg gtggaaagcc acatccgcat gatggcggcg
     3241 gtgcagccgt tcatctccgg cgcgatctcc aagaccatca acatgccgaa caaggccacg
     3301 gtgaaggaat gcggcgagag ctacctgcta tcctggaagc tcggcctcaa ggccaacgcg
     3361 ctctatcgcg acggctccaa gctgtcgcag ccgctggcca gccagatctt cgccttcgac
     3421 gacgacgaag acgagctgga agagacgctg gcggcccccg ccgccgcgcg cacctcggtg
     3481 gtcgccgagc gcgtggtcga gaagatcatc gagcgggtgt cgcgcgagcg cgagaagctg
     3541 ccgcagcggc gcaagggcta tacccagaag gccatcgtcg gcaaccacaa ggtctatctg
     3601 cgcaccggcg aatacgagga cgggcggccg ggcgaaatct tcatcgacat gcacaaggaa
     3661 ggcgccgcct tccgttcgat gatgaacaat ttcgccatcg cgatttccat cggcctgcaa
     3721 tacggcgtgc cgctggagga attcgtcgac gccttcacct tcacgcgctt cgaaccggcc
     3781 ggcatcgtga tcggcaacga ttcgatcaag aacgccacca gcgtgctcga ctacatcttc
     3841 cgcgaactgg cggtgtccta tctcgggcgc aacgatctgg cccatgtgcc gcctcaccac
     3901 aacgaggatc tcggcggcgg cgtggccgaa ggccagcgcg cggcgaaagg cgagatgcag
     3961 acggtcaacc gcaccacctc ggtgggctat gtgcgcggca atttccatcg catcgccgtg
     4021 gtgcgcggcg gcgcggcggc caagatgatg gaactgccag agccggagcg cgagaacgat
     4081 ctcggtaacg gccgttcgca cgatcacgac gacgtgcgcg aattcctcga agccggctcg
     4141 gtggatttga gcgagatcgc gtccagcgtc gaagcgactc tggcctccgc cggcgtctcg
     4201 acggcgaatc cggtgggcgt gcagatggcg gccatcgaga cgcggctggc cgcgagcgcg
     4261 caagacgtcc gcgcccaacg cgccgccgaa gcgcgcatga agggctatga cggcgatgcc
     4321 tgcggcgcct gcgggaactt caccctggtg cgcaacggca cctgcatgaa gtgcgacacc
     4381 tgcggcggca cgagcgggtg cagttgaggg agtcctcttt tgctatttcg tcgtgaccgg
     4441 gcttggccct actttaccct tccgttcgtc atggccgccc ttgaggcggc catccagtcc
     4501 ttaagcggta gcgagccgga agaaaaatat ggatggccgg gtcaagcccg gccatgacga
     4561 agtaggggaa tgcgtcagaa caaatccccg tacaaatccc gccagtcggg attcattttc
     4621 tcgatcagcg ccagcttcca ctttctcgac cagcgtttga ggctggtctc gcgctggatc
     4681 gcgtcggtga tcagcgccca ctcttcgtac cagaccagca tcgtcacttt gtagcgcttg
     4741 acgaagtcag agccgtgccc ttcgcggtgt tgggtgacgc gctcaagcaa attactcgtc
     4801 acgccgagat acagcgtgcc gttgcgtttg ctcgccatga tgtaaacgaa cccccggcgc
     4861 atttacgaag tctcgaaggc tggatggccg ggtcaagccc ggccatgacg aaagtggggg
     4921 atgattagta ccggcctttc ctttcgtccc ccacccgatc cggcttcact tcgttacgcc
     4981 ggatcggcct ccccacaaag gggaggtaaa gaaccaggca cggggcttgc ggtgtcaggg
     5041 ggtacgcgtt tcaaaacgag acaggcccat gatccgcact gcccttgccg cccttgccct
     5101 cgccgcgtgc gccgccacgg ctgcttacgg ccaggacaag cagaccgcct atctggacgc
     5161 cgtcgcctcg atcaaggccg ggaaccgcga ctgccccaat tgcgtcctgg cgggcgccga
     5221 cctgaccaat cagtgcgtca aagggggaaa tctgaccggc gccgatttcg accatgcgcg
     5281 cctggtcttg atgtgcatga gctattccga tttcaggaac gccaccttcc gcggcgccga
     5341 tctttccggc gccaacctcg cccatgccaa gctcgacggc gcggatttca ccggcgccag
     5401 actgaccatc acctcgatca agggagccga cctgtcgcac gccaaaggtc ttacccagag
     5461 ccagctcgat gccgcctgcg gcgacgccga gaccaaggcg cccgccgggc tacaggtcaa
     5521 aacctgttcg taaccggttt tcttcagcct ccgaaggaca tggccttggc cccggctgcg
     5581 tagccggggc tttttcttcc gctgacgccg ccccctccgc ctcccccgct atgcgggggc
     5641 ggaaagggac ggggctattc cgcggcgtgc ggcaagccct tcggcttcca gcgcagcacc
     5701 ggcttgcgcg aggcggcggt ttcgtcgagg cggcggcgcg gcgtgaactt cggcgcccca
     5761 tcgaagcgct cgcgttggcc ccccttggct tccagcgcca gcgtccgcag cacgtggatg
     5821 aagcggtcga ggctttcctt ggattcggat tccgtcggct cgatcagcat cgcgccgtgc
     5881 accacgagcg ggaaatagac cgtcatcggg tgaaaaccct cgtcgatcat cgccttggcg
     5941 aaatccagcg tgctcacccc tgtgtctttc agaaagcggt cgtcgaagag cgcctcgtgc
     6001 atacaggggc cttcatacgg cgcgctcatc acgtctttca gcgaggccag gatgtagttg
     6061 gccgacagca ccgcatcttc gctcgcctgg cgcacgccgc cgcggccatg gctgagcatg
     6121 taagcaagcg cgcgcacgaa catgcccatc tggccgtgga aggcgcacat gcggccgaag
     6181 ggctgtgcct cggcgggcgc atcggcgcgg tcttcgatca ggcggaagcc gttcacctcc
     6241 tgtaccaaga gcggcaaggg cgcgaacggc gccagcgccg ccgacagcac caccggaccc
     6301 gcgcccggcc cgcccccgcc atgcggggtg gagaaggtct tgtgcaggtt gatatgcatg
     6361 gcatcgacgc cgagatcgcc cggccgcacg cggcccgcga tggcgttgaa gttggccccg
     6421 tcgcaataga aataggcgcc cgccttatgg acgagaccgg cgatctccac cacgtcgcgc
     6481 tcgaacaggc cgcaggtgtt gggattggtc agcatcaagg cgccgacatc cgggcccagt
     6541 ttcgccttca aggcttcgac atcgacgcgg ccgtcgggcg tggccttgac ttcatcgacg
     6601 gtgaagcccg cgaacgcggc cgtcgccgga ttggtgccgt gcgccgattc cggaaccagc
     6661 acgcgcggcc gcttttcgcc gcgcgccagc agcgcggcgc ggatcgtcaa catgccgcaa
     6721 agctcgccat gcgcacccgc cttgggcgac atcgccaccg ccggcatgcc ggtgagcgtg
     6781 gtgagccagc gcatcagttc cgcgatcagc gccagcgcgc cttgcgccgc cgactgcggc
     6841 gccagcggat gcagatcggc gaagccgggc agccgcgcca gcttctcgcc caggcgcgga
     6901 ttgtgcttca tggtgcagga gccgagcgga taaagcccgg cgtcgatggc gtagttcatc
     6961 cgcgacaggc ggacgtaatg gcgcaccact tccggctcgg aaagtcccgg caggcctatg
     7021 ggccctttgc ggcgctgccc cgccagacgg ctgtcaccga cgctgacctg cggcaagtcc
     7081 acgccgcaca tgcccgcacg gcccagttcg aagatcagct tttcttcatg gtcgaggccg
     7141 cgatcgccag agatggtggc aacagtcatg acagcacctc cgcgagcgcc tcggcgaagg
     7201 cggcaaagtc ttcaccagtg ttggtttcgg tggcggcgac gatcagcaaa tcacgcgcgg
     7261 cggcttcgtg cggcaacagc cgcgaggcgg gcacgccggc gagaatgtct ttcgccgcca
     7321 gcgcttcgac aatttcggcg gcgggcctgg gcaagcggag cgtaaattcg ttgaagaagg
     7381 acggcgtaac cagcgagacg cccttcacct tcaccagccg atcggccaag gccgacgcct
     7441 tggcgtggtt gaggcgcgca agctgcgcaa atccttcctc gcccagcagc gacagatgga
     7501 tggtgaaggc cagcgcgcac agcccggaat tggtgcagat gttgctggtc gccttctcgc
     7561 ggcggatatg ctgctcgcgg gtggagagcg tcagcacata gccgcgccgg ccttccgcat
     7621 ccaccgtttc gcccgacaag cggccgggca tctggcggat gaatttctcg cgcgtcgcaa
     7681 acaggccgac ataaggcccg ccgaaattga ggccgttgcc gatggactgg ccttcggcgg
     7741 cgacgatgtc cgcgccttgc gttcccggcg gctcgatcag gccgagggaa acgatctcgg
     7801 tcaccacggc gatcaagagc gcgcccttgg catgagcggc gtcggcgacg ggttgcaagt
     7861 cgcggatcag gccgaacacg tccgggcttt gcaccaccac gcaggcggtg tcctcgtcga
     7921 tcaaggcggc gatgtcttcg gcgcggccgg gcgtcaccgg cgcacgcacc accttgcccg
     7981 acagattggc gacggtttcc accgtctcgg cgtaatgagg gtgcagcccg cccgacaaga
     8041 ccgccttgga gcgacgggtc aggcgttgcg ccatcagcac ggcttcggcg gtggcggtgg
     8101 aaccgtcgta aagcgaggcg ttggccactt ccatgccggt gagcagcgcc acctgggtct
     8161 ggaactcgaa gagatattgc agcgtgcctt gcgagatttc cggctggtag ggcgtgtagg
     8221 aagtaaggaa ttcggagcgc tggatcagat gatccacggc ggccgggaca tgatgcttgt
     8281 aggcccctgc cccgcagaag aacggccccg cttccgccgc cacgttgcgc gacgcgaggc
     8341 acgacagggc gcgctcgacc tccagttcgc cttgcgtgtc cggcaggtcg aaggccgaca
     8401 gcggcaccac cgccgatttc ggcacgtcgc ggaacagcgc gtcgatgtcc ggcacgccga
     8461 ttttcgccaa catcgcggca cggtcgcttg gagttaaagg caaatagcgc atcacaaatc
     8521 ccataccttg cggacgtcat ctggatggcc gggtcaagcc cggccatgac gaaaaaaagt
     8581 tagaggcctt tcacatactc atcgtattgc gccctggtca tcagggtgga gagttccgcc
     8641 gcatccgcca gcttcagctt gaagaaccag ccttcgccct cgggatcggc attgaccttg
     8701 gcgggatcgg cgttcagcgc ctcgtttacc gccgtcacgg cgccgccgac cagcgcgtag
     8761 atctcgctgg ccgctttcac gctttccacc acggcggctt cggccccctt ggcgacgtcg
     8821 cggcc
//
LOCUS       DASWPU010000114         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_25232,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000114 DASWPU010000000
VERSION     DASWPU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25232"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VGC27_02590"
     CDS             complement(<1..746)
                     /locus_tag="VGC27_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0281493.1"
                     /translation="MNIHEYQAKEILRSFGAPVPRGKPAFSVEEAVAAARQLPGPVWV
                     VKAQIHAGGRGKGGGVKVVKSVEEVEKEARRMLGMTLVTHQTGPQGREVKRLYIEDGS
                     AITRELYLSALVDRAASRVAFIASTEGGMDIEEVARRTPEKIATFQVDPASGYSPFIG
                     NRIAAALKLKGDEVKQCGALVKSLYDAFLAKDMSLLEINPLVVTKAGLLVCLDAKMNF
                     DDNALYRHKDIVALRDLTEEDPIEVEASKH"
     gene            complement(848..>1614)
                     /locus_tag="VGC27_02595"
     CDS             complement(848..>1614)
                     /locus_tag="VGC27_02595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY0281494.1"
                     /translation="TLSFPAVFVNLIHGQNGFLTAALFAGAIALLDKRPIVAGILFGL
                     LAYKPQFGILIPLVLAATGRWRGFAAAGATVASLALLATLMFGAEIWSAFFASTHFSR
                     TTVLEQGGTGFEKMQSVFAAIRLWGGPIDLAYASQAVATLGVAAALVVLWRSGASMAA
                     KGAGLCLGALLATPYCFDYDMMLLAPAIALLAADGGARGFFPYEKTVLAALWLVPIAA
                     RGVADATYIPLGLIVMILAFAFVVRGGARQTAPALA"
BASE COUNT          314 a          523 c          513 g          264 t
ORIGIN      
        1 tcgtgcttcg aggcttcaac ttcgatgggg tcttcctcgg tgaggtcgcg cagcgcgacg
       61 atgtccttat gccggtagag tgcgttgtcg tcgaaattca tcttggcgtc gaggcagacc
      121 agcaagccgg ccttggtgac caccagcgga ttgatttcca agaggctcat atccttggcg
      181 aggaaggcgt cgtacaggct tttcaccagc gcgccgcatt gcttgacctc gtcgcccttc
      241 agcttcagcg ccgccgcgat ccggttgccg atgaaaggcg aatagcctga cgccgggtcg
      301 acctggaacg tcgcgatctt ctccggcgtc cggcgcgcga cctcttcgat gtccatgccc
      361 ccttcggtcg aggcgatgaa ggcgacgcgg ctggcagcgc gatccaccag ggcggaaaga
      421 tacagctcgc gcgtgatcgc cgaaccgtcc tcaatataaa gccgcttgac ttcgcgtcct
      481 tgcgggccgg tctgatgggt caccagcgtc atgcccaaca tccgccgcgc ttccttttcc
      541 acttcctcga cggatttcac caccttgacg ccgccgccct tgccgcgccc gccggcatgg
      601 atttgcgcct tcaccaccca caccgggccg ggaagctgtc tggccgccgc gaccgcttct
      661 tcgacgctga acgccggttt gccgcgcggc accggcgcgc cgaaagaacg caaaatctct
      721 ttggcctgat attcgtgaat gttcatggaa ggaccttgtc gggaacggag acgcgcggaa
      781 gtgcttggaa caggccgcag aatagcagcc gaccttgacc acgcaagcgc ttcgcgccgg
      841 tcagggctca ggcaagcgcg ggagcggttt ggcgcgcgcc tccgcggacg acaaacgcga
      901 aggcgagaat catcacgatc aggcccaagg gaatataggt tgcgtccgcc acgccgcgcg
      961 cggcgatcgg aacgagccag agcgcggcca gcaccgtctt ttcgtaaggg aagaacccgc
     1021 gcgcacctcc gtcggccgcc aagagcgcga tggcgggagc aagcagcatc atgtcgtaat
     1081 cgaagcagta gggcgtggcc agcagtgcgc ccaggcacag gcccgcgcct ttggcggcca
     1141 tgctcgcccc agaacgccac agaacgacca gcgccgcggc gacgccgagc gtcgccacgg
     1201 cctgagatgc gtaagccaga tcgattggtc cgccccacag ccgtatggcg gcgaagacgc
     1261 tttgcatttt ctcgaagccg gtaccgccct gttcgaggac cgtggtgcgg ctgaaatggg
     1321 tggaagcgaa aaaggcggac cagatctccg cgccgaacat cagcgtggcg agaagcgcca
     1381 atgacgcgac ggtcgccccg gcagcggcga agccgcgcca gcgccccgtc gcggcgagca
     1441 ccagcggaat caaaatcccg aattgcggct tgtaggcgag aaggccgaac agaattcccg
     1501 caacgatcgg gcgcttgtcg agcagcgcga tggcgcccgc gaacaacgcg gcggtgagaa
     1561 atccgttctg cccatgtatg aggttcacga acacggccgg aaaagacagc gtgt
//
LOCUS       DASWPU010000115         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_62186,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000115 DASWPU010000000
VERSION     DASWPU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62186"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VGC27_02600"
     CDS             complement(<1..974)
                     /locus_tag="VGC27_02600"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0281495.1"
                     /translation="MSSPTENAPWPRDGDPLEDAERLIAENRAGEAAELLRLRLAEGR
                     GGLLARLAYANALDAAGDKAGALAAARETAMLHPGVAVAALGLGKILLKAEQLPTAIA
                     EFQRALRLDPDLDEARLLLGRAWLAAGEADKALEAFAALPPKTAGLADEIARAEAIRA
                     RPRSDAGYVRHLFDQFSADYDARMLSQLSYRAPQILRALAGMVMPGRRALTILDLGCG
                     TGLSGAAFKDLAARLDGIDLSPAMIAKARARGLYDSLAVADIESALAAGRTDLVLAAD
                     TLVYLGDLEATMRGVARALRPDGFFLFTVEKKEGEGFELGPKRRWRHS"
     gene            complement(937..1791)
                     /locus_tag="VGC27_02605"
     CDS             complement(937..1791)
                     /locus_tag="VGC27_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY0281496.1"
                     /translation="MPIHYDEIMQLKSVGDEFRYTDRETMLYALGVGFGRDPLNRQEL
                     KFVYENDLQTVPTFATVLGWGQATLAPSGLNYLMVVHGEQRLTLHKPLPAQAEIIADE
                     RVTGAFDKGEGKGALILLEKVIREKHSGDKLCTLGTTYFARGDGGFGGPRDGAPAPHP
                     LPERAPDLAVECDTRPDQVFLYALSGDRNPLHRDPSVAALAGYPRPILHGLCAYGTAC
                     RAILSGVCGYDAKKIVGFDARFSAPVFPGETIVTEIWVDGSVVSFRGKLKEREAVVLT
                     NGKCTLAA"
     gene            1912..2367
                     /locus_tag="VGC27_02610"
     CDS             1912..2367
                     /locus_tag="VGC27_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281497.1"
                     /translation="MSPSYEQAPPRRRSGCLWGCLGLLLAVVILLGGVFGYTAWFLHK
                     SLDGDPHLQTIMAIVRDDARAEAVLGRNIKLVEVEDRTFAMGTGIGKSASYILRLIGS
                     NGEGVLKVKLDLSDGKTNVTLMILTGPDGLSHILAGKEPESPLQQQQSI"
     gene            2665..2961
                     /locus_tag="VGC27_02615"
     CDS             2665..2961
                     /locus_tag="VGC27_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281498.1"
                     /translation="MSRWIMLGVGIVAAVVLMGTYASYLPNPLNIIAPCENTVVKSVV
                     SPDGRHKAVLFQRNCGATTGFSSQVSIIPASLWLPDHSGNAFVADLPENKSPAA"
     gene            complement(3075..>3628)
                     /locus_tag="VGC27_02620"
     CDS             complement(3075..>3628)
                     /locus_tag="VGC27_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0281499.1"
                     /translation="TSGRIHIHRTGLVWRALRASVALPGILPPVTHHGHLLVDGGVMN
                     NLPVDVMRERNAGPLIASDVTGDMDMSVRDGRYSERPVWSLLWHRMRGTPSIASILMR
                     AGTAGSEAQRRLVREQADYLFEPPLAGVEPLDWKAFDRAIAGGYTHAKLMIDKNGVPL
                     TEAWSEGPAVAAARHGEDDQSVL"
BASE COUNT          637 a         1191 c         1162 g          638 t
ORIGIN      
        1 tcggaatgcc gccagcggcg cttggggccg agttcgaagc cctcgccctc cttcttctcg
       61 acggtgaaaa ggaagaatcc gtccggcctc agggcgcgtg cgacgccccg catggttgct
      121 tcgagatcgc ccaaatagac cagcgtgtcg gccgccagca ccagatcggt gcgccccgcc
      181 gccagcgcgc tctcgatatc cgcgacggca agagaatcgt agaggccgcg cgcccgcgct
      241 tttgcgatca tcgccgggct gagatcgatg ccgtccagcc tcgccgccag gtccttgaag
      301 gccgcacccg acaggccggt gccgcagccg agatcgagaa tcgtcaatgc ccggcgtccc
      361 ggcatgacca ttccggccag cgcgcgcagg atttgcggcg cgcgatagga aagctgcgac
      421 agcatgcgcg cgtcgtaatc ggcggaaaac tggtcgaaga gatggcgcac atagccggcg
      481 tccgaacgcg gccgggcgcg gatcgcctcc gcgcgggcga tttcatcggc gaggccggcc
      541 gtcttcggcg gcagcgcggc gaatgcttcc agcgccttgt cggcttcgcc cgccgccagc
      601 caggcgcggc cgagcagcaa gcgcgcttcg tccaaatccg gatcgagccg cagcgcgcgc
      661 tggaattcgg cgatggcggt gggcagttgc tccgctttca gcaagatttt gccaagcccg
      721 agcgccgcca cggcgacgcc cggatgcagc atcgcagtct cgcgcgccgc cgcgagcgcg
      781 cccgccttgt cgccggcggc gtcgagcgcg ttcgcatacg ccagccgcgc cagcaagccg
      841 ccgcgccctt ccgcgaggcg caggcgcagc aattccgccg cctcgccggc gcggttctcc
      901 gcgatcagcc gttcggcgtc ttccagcggg tcgccgtcac gcggccaggg tgcattttcc
      961 gttggtgagg acaaccgcct cgcgctcctt gagcttgccg cggaacgata ccacggaacc
     1021 atccacccag atctcggtca cgatggtctc gccgggaaaa accggcgcgg agaagcgcgc
     1081 gtcaaagccc acgatcttct tcgcgtcgta gccgcaaacg ccggagagga tggcgcggca
     1141 ggcggtgccg taggcgcaaa gcccgtgcag gatcggtctt ggatagcccg ccagcgcggc
     1201 gacgctggga tcgcggtgca gcggattgcg gtcgccggac agcgcataga ggaagacttg
     1261 gtcggggcgg gtgtcgcatt cgactgcgag atcgggcgcg cgttccggca gtgggtgcgg
     1321 cgcgggagcg ccgtccctcg gtccgccgaa accgccgtcg ccgcgtgcga agtaggtggt
     1381 gcccagggtg cagagcttgt cgcctgaatg tttctcgcgg atcaccttct ccagcaggat
     1441 cagcgcgccc ttgccttcgc ccttgtcgaa ggcaccagtc acccgctcgt cggcgatgat
     1501 ttcggcctgg gcgggcagcg gcttgtggag cgtcagccgc tgctcgccat gcacgaccat
     1561 gagataattg aggcccgaag gcgccagggt cgcctgtccc cagccgagca ccgtggcgaa
     1621 ggtgggcacg gtttgcagat cgttctcgta aacgaattta agctcctgcc ggttcagagg
     1681 atcgcgcccg aagcccacgc ccagggcgta aagcatggtt tcgcggtcgg tgtagcggaa
     1741 ttcgtcgccg accgacttga gctgcatgat ctcgtcgtaa tggatgggca tggccgccgc
     1801 cgctcccgcg ctaacgtttc ctcactcggc gggacagact atggttgcgc gccggctgcg
     1861 gcaatcgccg cctcacgcct tcgttcgccg aaagacagcc ttgagggaag tatgagcccg
     1921 tcatatgaac aagctccgcc gcgccgccga tcaggctgcc tgtggggatg cctgggcttg
     1981 ctgctggccg tcgtaatcct gctcggcggc gtgttcggct acaccgcctg gttcctgcac
     2041 aagagcctcg acggcgatcc ccacctccag acgatcatgg cgatcgtccg cgacgatgcc
     2101 cgcgccgagg cggtgctcgg acgcaatatc aagctcgtgg aggtggaaga ccgaaccttc
     2161 gcgatgggaa cgggcatcgg caaatccgcg tcctatatct tgcgcctgat cggttcgaac
     2221 ggcgaaggcg tcctcaaggt caaactcgat ctgagcgacg gaaagaccaa cgtgaccttg
     2281 atgatcctca ccggcccgga cggtctttcg cacatcctcg ccggcaagga acccgaaagc
     2341 ccgctgcaac agcagcagtc gatctgactc gccgcctgcc caactttgcg aatttgttgc
     2401 cggtcagaaa aggagtaaag cagattggca tcgaggtgac tcgatgtcac cccggtgtca
     2461 tggcccgcga atgcgggcca cccaggtgac atctgcgcac gtcccagggg attttgctgc
     2521 gctgttcttc aaagatttct tcagacttca actggatggc ccgcattcgc gggccatgac
     2581 aagttagtat ttgattccgt ctgactacag cctgttctag aagattggct gtcgtggcct
     2641 accaagtcct cgaaggaggt gcacatgagc cgatggatta tgcttggcgt tggcatcgtg
     2701 gctgcggtcg tgttgatggg aacatatgcg tcctatctgc caaatcccct gaacatcatc
     2761 gctccttgcg aaaataccgt ggtcaagagc gtggtatctc ccgatggccg acacaaggcg
     2821 gtcctcttcc aacgaaattg cggagcaacg accgggttct cgtcccaggt ctcgatcata
     2881 ccggccagcc tttggcttcc cgaccatagc ggaaacgcgt ttgttgcgga tttgcctgaa
     2941 aacaagtctc cggcagcata gggtggaccg cgcgtcgcca tacgatggcg aacagggaat
     3001 agtcttgaga tacgctacga tcatgccgct cgcgtattcc atcaagagaa gcggatcaaa
     3061 gagatcgctg tctcctacaa aacggattga tcgtcctctc catgccgcgc cgccgccacc
     3121 gcggggcctt cgctccacgc ctcggtgagc ggtacgccgt tcttgtcgat catcagtttg
     3181 gcgtgggtgt agccgccggc aatggcgcgg tcgaaagcct tccaatcgag cggctccacc
     3241 ccggccagcg gcggctcgaa gaggtaatcc gcctgctcgc gcaccaggcg gcgctgcgct
     3301 tccgatcccg ccgtgccggc gcgcatcagg atcgaggcaa tcgacggcgt gccgcgcatg
     3361 cgatgccaga gcagcgacca caccgggcgt tcgctgtagc ggccgtcgcg caccgacatg
     3421 tccatgtcgc cggtcacgtc ggaagcgatc agcgggccgg cattgcgctc gcgcatcacg
     3481 tccaccggca gattgttcat caccccgccg tccaccagca gatggccgtg atgggtcacc
     3541 ggcggcagaa tgccgggcag cgccacgctg gcgcgcagcg cccgccacac cagcccggtg
     3601 cgatggatgt ggatgcgccc gctggtga
//
LOCUS       DASWPU010000116         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_104943,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000116 DASWPU010000000
VERSION     DASWPU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104943"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VGC27_02625"
     CDS             <1..230
                     /locus_tag="VGC27_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281500.1"
                     /translation="LEQHKIACDASSDKDIRVFGQENVQKRYVIEFRCSQRKAGTVAF
                     IPLSGNNAPFEVLDCAAADKRKIKCELTGSY"
     gene            complement(476..1063)
                     /locus_tag="VGC27_02630"
     CDS             complement(476..1063)
                     /locus_tag="VGC27_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281501.1"
                     /translation="MKTIALALLTLALAFAPAHAQATQQSDGSEIMNKVVNVPSPTSW
                     TALGLAQPKQLEDDKVQGGMAMRFAVPGKGVNPWDVTARSAIIKPVKSGDVVLLAFWA
                     RAEVPAEGQQTAILPGIRIEETVAPYLALAQDAANVTGTWAMYYASGVATKDYKPGSL
                     TVTLHLAAAKQTVDLGPVFVVDLGPDYDRSKLPHN"
     gene            complement(1260..1913)
                     /locus_tag="VGC27_02635"
     CDS             complement(1260..1913)
                     /locus_tag="VGC27_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0281502.1"
                     /translation="MKRLRLSVPVLVAALVLLPLAAHAEPSAFDQATQLELDGMDLMQ
                     KGDLDGAITKYRQAVQIYPKSKAFRQNLAEALNNAGVAKYQAKDYPAAISDFQEALAN
                     VPNFAQAKTNLALAQGDQFNAEGLVLFKAGDFAGAVQKFNEALVAEPGYKNAIVNRDA
                     AEAEIALKNGDLATAVAKLQEAVSIASTPFLQNKLETAQAALAAQQEAAQKAQQKTK"
     gene            complement(1978..2127)
                     /locus_tag="VGC27_02640"
     CDS             complement(1978..2127)
                     /locus_tag="VGC27_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281503.1"
                     /translation="MRAGFAGERRLKIGLSATTRANLMTVVPLAGPHLQPCGDKTLAC
                     LPVLI"
BASE COUNT          437 a          718 c          707 g          450 t
ORIGIN      
        1 ccctcgaaca acacaagatc gcctgcgatg ccagcagcga taaagacatt cgtgtcttcg
       61 ggcaggagaa cgtccaaaag cggtatgtca tcgaatttcg ctgctcgcag aggaaagcgg
      121 gaacggtggc gttcataccg ctgagcggca ataacgcgcc gttcgaagtg ctcgactgcg
      181 cggcggcgga caaacgcaaa atcaaatgcg aattgacggg ttcgtactga cgcacaaacg
      241 cttgccgcaa gcgtgaggcg tttccagtgt ttccccccag cctcagacgg caaaggccgc
      301 cgccgccgcc ctttgccgtt gctatgcgca aagcctggcc gcccggcaca tcggaggccg
      361 gaacgagccg accgaggcga cggagggatc gttgatcaac cggccgcgca ccggcagaag
      421 cgcgcggatt cgggcgagtt cgatagcggg tggtgcgtac ttcgaaacca cggcactagt
      481 tgtgcggcag cttggagcgg tcgtagtcag gtcccagatc gaccacaaac acggggccga
      541 gatcgaccgt ctgcttggcc gccgcaagat ggagcgtgac ggtcagcgac ccgggtttgt
      601 aatccttggt tgcgactccc gaggcgtaat acatcgccca ggtgccggtg acgttcgcgg
      661 catcctgtgc cagggccaaa tagggcgcga cggtctcttc aatgcggatg ccggggagga
      721 tggccgtctg ctgtccctcg gccggcactt cggctcgcgc ccagaacgcc agcaaaacga
      781 cgtcgccgct tttgaccggc ttgattatcg ccgagcgcgc cgtgacgtcc catggattta
      841 cgcccttgcc gggaacggcg aagcgcatcg ccatccctcc ctggacttta tcatcctcca
      901 attgcttagg ctgggcgaga ccaagcgccg tccaagacgt cggcgacggt acgttgacca
      961 ccttgttcat gatctccgaa ccgtcgcttt gctgggtcgc ctgcgcatgc gccggcgcga
     1021 acgccaacgc cagagtcagc aatgccaatg cgatcgtctt catggtttcc cccctgcccg
     1081 gcgcttcgtc agccggtttc ggtcattata acagacttga gagggatggc acgataggca
     1141 ggggccgatc gaccgcaggc ccctcgcggc cgtggcgatc aacacctgac cgccgccaat
     1201 cgatgcgatg cggcgccggt ctttccagcg ggagatttcg tgccgccgta tcagccgcac
     1261 tacttggtct tctgttgcgc cttctgggcc gcttcctgct gcgccgccag cgccgcctga
     1321 gccgtttcaa gcttgttctg aaggaacggc gtcgatgcta tggataccgc ttcctgcagt
     1381 tttgccacag cggtcgccag atcgccgttc ttcaaagcga tctcggcctc ggcggcgtcg
     1441 cgattgacga tcgcattctt gtagccgggc tctgccacca gcgcttcgtt gaatttttgc
     1501 acggctccgg caaaatcacc ggccttgaac aacaccagcc cttcggcgtt aaactgatcg
     1561 ccctgagcga gggcgagatt ggtcttggcc tgggcgaagt tcggcacgtt tgcaagcgct
     1621 tcctgaaaat cgctgatcgc cgcggggtaa tccttcgctt gatatttcgc gacaccggcg
     1681 ttgttcaggg cttcggcgag attttgacgg aaggccttgc tcttgggata gatttggacc
     1741 gcttggcggt atttcgttat ggcgccgtcg aggtcgcctt tctgcatcag gtccatgccg
     1801 tccaactcaa gctgcgtcgc ctgatcgaaa gccgacggct ctgcgtgcgc agcgagcggc
     1861 agcaagacaa gagcggccac gagaaccgga acagataagc gcagacgttt cattgtgttc
     1921 cccccaattc tgtagcgcag tattcctccc cacaaggacg atcagtccgc ggcaaggtta
     1981 gatcaaaacc ggcaaacagg ccaaggtttt gtcgccgcag ggttgcagat gcgggccggc
     2041 cagcggcacg acggtcatga ggttggcgcg cgttgttgcg ctcaagccga tcttcagccg
     2101 ccgttccccg gcgaaacccg ccctcattcc ggccggcgcc gcaccggcaa atgaacccaa
     2161 cattgggacg caaaccgtgc gccgcgaacg aagaatcggt ttagcaaccg gtgctctgaa
     2221 cgattgtctt ggaatccgga tctctcggat gcggccatcc ggaggcgccg gcagcgcgaa
     2281 ggcggtcgcg gattgatcgc aggcgcgctc ag
//
LOCUS       DASWPU010000117        11737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_43878,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000117 DASWPU010000000
VERSION     DASWPU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11737
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43878"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1194)
                     /locus_tag="VGC27_02645"
     CDS             complement(169..1194)
                     /locus_tag="VGC27_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY0281504.1"
                     /translation="MTEQQWNELLAAIRGEQKRPLPVAFIIDCPWLPNWYGVNILEYF
                     QSDEVWFAANKKAIDTFPEAIFLPGFWSEFGMCTEPSAFGCKCQFPENEFPFAEKNVL
                     SEEDVDNIVQPNVEIDGLLPFMLNRLTRNRSRIEAMGHQIKFSVSRGPLNIASFLMGT
                     TEFLMMLGTDPERTHKLLRVINTFLMDWHARQRQAVDSIDGMLVLDDIVGFVSEKDYL
                     EFAAPYLKELSDAPVAVKLFHNDADCRQSVKHYPDLGINLYNPGIHMTMNELHRVTGN
                     RLTILGNIPPRDVLAQGTPDGVRDAVRRMLAETTDHSRMLPSCAGGMPPAVSTANLRA
                     FIEGIAG"
     gene            1393..2274
                     /locus_tag="VGC27_02650"
     CDS             1393..2274
                     /locus_tag="VGC27_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY0281505.1"
                     /translation="MAHPLIQELSRRAPVITDGAWGTQLQARGLEPGEVPDLWNLSHP
                     KEVAAVARAYVEAGSQIILTNTFGANRFRLAESDGAGRLAEINKAGVALSRRAADKKA
                     LVFASLGPSGKLMMNEEVSEEELRAAFAEQAEALAAAGPDGIVVETMQDLAEAKIATE
                     TAKATGLPVVVCMVFDSGKDKDRTMMGTTPEQAAAGLIEAGADAVGANCGQGIAGFVN
                     ICARLRAAAEGHPVWIKANAGLPEIRDGKTVYEMTAAQFASYVPAVLTAGASFIGGCC
                     GTTPEFIRDIHRLLRGP"
     gene            2274..3029
                     /locus_tag="VGC27_02655"
     CDS             2274..3029
                     /locus_tag="VGC27_02655"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEY0281506.1"
                     /translation="MDRLRLHVIACSSLRPELEMLAAQAKTAVTLRHLEMGLHQRSAE
                     TLRAVLQGAIDAETIDNRCDAVALGYGLCNQGIVGVQARRMPVVIPRAHDCIGMLLGG
                     SRQYLAQLEAQPGTYFQSAGWLENAPAEGDVRQPDFTFGPNSNVTRERLAERYGDDNA
                     DYLLEQFDGFTRHYERLAYIATPAPASARWEDAAKETAAKRNLTFERLKGDLGWLRRL
                     VNGEWNANEFLTLQPGERVVLTFDERLIGAVPA"
     gene            3026..4522
                     /locus_tag="VGC27_02660"
     CDS             3026..4522
                     /locus_tag="VGC27_02660"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEY0281507.1"
                     /translation="MTKPTCELALSPAGRRLKVAAGMALRDVLFEQGVEFPCGGHGRC
                     KGCRVRLLKGHLEPGAEEARVLTQAELADGWRLACRHFIAGDLELELAQWEMPILSDH
                     SAFAFTPRAGLGAAVDLGTTTIAAQLLDLATGNVLAARTALNAQARHGGDVISRVEYA
                     LRGGQGELERLVREQIGRMIGQLLDSTPPEQELVRIVLVGNTVMHHLFCGLDVTPLAT
                     HPFEPEHPGRIRFSTAQLGWGGNTAVEFMPCIGGLVGSDILAGIVATGLGQRDGLGAL
                     IDLGTNGEVVVGGRDRLVCTSTAAGPAFEGARIAMGMRAATGAISEVRAIDGAIVCRI
                     IGGGAARGLCGSGLVDAVAAGLNLGWIEAGGRMTLGSMPLSGPVALIPQDVRELQLAK
                     GAIAAGLRMLAARLGVAPGEIGQLHLAGAFGNTVGRVSARRIGLLRLPVERIVPAGNA
                     ALLGAKRALFEDPVLWDGLAKRIEHVALNEEPAFQDIFAEEMRFPERA"
     gene            complement(4681..5313)
                     /locus_tag="VGC27_02665"
     CDS             complement(4681..5313)
                     /locus_tag="VGC27_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEY0281508.1"
                     /translation="MSDVKSLHEAILTGDVPAAKAATEAALAAGVAPLAVVQETMMPA
                     MDEVGRRFECNEYFVPELLLAARAMKAGLELIRPLLAAGGVEPVGRIAIGTVKGDLHD
                     IGKNLVAAMLEGGGYEIIDLGVNVDPAKFVAAVKDKGAQIVAMSALLTTTMPAMKTTI
                     EALKKAGVRDQVKVMVGGAPISEAYAKEIGADGYSHSAASAVADARGLYA"
     gene            complement(5480..7540)
                     /locus_tag="VGC27_02670"
     CDS             complement(5480..7540)
                     /locus_tag="VGC27_02670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0281509.1"
                     /translation="MTRMLRLAASAFALLLAAAALAPASAAVPFTAKLMASLERIDDP
                     RLSPDGRYVLYALRTVDYAANKAARSLWVLDLKTHASRRLKISDGGATSGRWSPDGSI
                     YFLSGRAGGIDQVFRTDLAGRKAVQVTSVPLSVGAFKLAPDGKVLVISLAVFPDCPDL
                     ACTKKRLDATKTGKATGVVYDRLLVRHWDTWADGTRNHLFALALNTKSARPVPLMTGF
                     DGDVPSKPFGGDEDFAIAPDGKLVVFSAKSGRDEAWTTNFDLWEAPLDGSAPPRDTTE
                     ANQAWDAAPVFSADGKWAAYRAMKRPGFEADRFAIMLHDLTTGAVREVAPAWDRSPGS
                     LAWSPDGGTLYATAEEMGQSKLFAIDVASGKVKPLTGEGRVGGFDVGPDGIVFARDSL
                     TGPAQLFWVGLDGGAPVKLTNINGEALKDVHWGEPEQFAFKGWNDETVHGYVIPPADP
                     KPGLKYPVAFLIHGGPQGSFGNAWSYRWNPQIYTNAGYAVVMVDFHGSTGYGQAFTDS
                     ISRHWGDRPLEDLQKGWAFALSKYDFLDGNRACALGGSYGGFMVNWVAGVWNEPWKCL
                     VNHDGTFDTRGMGYATEELWFDEWEQGGPFYEWREAAEKFNPYNHVKSWSKPMLVIHG
                     SRDYRIPLTQALGTFTALQRRGIASKFLYFPDENHWVLKPQNSAQWYAEVLGWLDRWT
                     KTDK"
     gene            complement(7842..8300)
                     /locus_tag="VGC27_02675"
     CDS             complement(7842..8300)
                     /locus_tag="VGC27_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0281510.1"
                     /translation="MASPRAVAVKRSTGIQYAALPYRVSGGTLEILLVTSLRTRRWII
                     PKGWPIDGRRPFACAALEAWEEAGVSGDMEKTAIGQFQYFKQIKKGVNLPCRVEVFAL
                     KVTRERKTWPEKGGRVRRWCTVAEAAATVEEPQLRLLIAKFGAQMTAGLR"
     gene            complement(8309..9301)
                     /locus_tag="VGC27_02680"
     CDS             complement(8309..9301)
                     /locus_tag="VGC27_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEY0281511.1"
                     /translation="MSEPLLIALIAAALLFDFLNGLHDAANSIATIVSTRVLAPRYAV
                     AWAAFFNFIAFLVFGMHVARTIGTGIVTPDIITDQVIFGALMGAIVWNVVTWLAGIPS
                     SSSHALIGGLVGAGVARSGWSVIEWGGLGKTIGAIFLSPGLGFVLALMLILGVSWSFM
                     RVTPARVDGIFRKVQFVSASLYSLGHGGNDAQKTMGIIAALLFAHGALKGDFYVPFWV
                     VLSCQAAMALGTLCGGWRIVHTMGSRITRLAPLQGACAETAGSITLFMATGLGIPVST
                     THTITGAIVGVGAARRTSAVRWNVAKDIVIAWFVTMPAAGIIGAVSFYLFGVLG"
     gene            complement(9317..9958)
                     /locus_tag="VGC27_02685"
     CDS             complement(9317..9958)
                     /locus_tag="VGC27_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEY0281512.1"
                     /translation="MLWLQALMPKEERFFDHFNRHARTIVNGSEALNALLHDGATVPE
                     ACRKVMDFENQADAITRDVLILTRRSFITPFDRSDIKELISSLDDSIDQMQKTAKAIL
                     LFEVSVFERQMADMGDIIVQAAKLTLDAVALLGAHRQQASRLIGITGEIIRLEEAADE
                     LHNQGIKVLFVKHRGGSAMEFIVGTEIYDHLEKVMDRFEDVANCISGIVIEQA"
     gene            10501..11583
                     /gene="galK"
                     /locus_tag="VGC27_02690"
     CDS             10501..11583
                     /gene="galK"
                     /locus_tag="VGC27_02690"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY0281513.1"
                     /translation="MSTFTAKAPARANIIGEHTDYTTNQGHVLPTPLSFHTTVGVQEI
                     AGAAGRMTISSENYGNQPFERSTRDEPRRHWTDYVVGCVRVVAKKTELPALEIKIKSD
                     VPIGSGISSSAALCVAVVRALKGLSGIELSGEQIALMAHQAEHDYVGVPCGKMDQFVA
                     SLGRPGEALLLNTRNLAYRHYPLAANTAIMLVDCGKKHDLANGSGYKQRVAECTEARH
                     RLGVTTLCELSYADLDRLAALPDLIRRRARHVISENLRVLEFVSALEKGDLEKQADLL
                     AASHVSQRDDFEVSIPEIDALVEASIRFGVRGARIVGGGFGGAIIALVPREQAVAWWS
                     FVAKENPNSQLISTYALKNSENEYAA"
BASE COUNT         2171 a         3757 c         3763 g         2046 t
ORIGIN      
        1 agtggtcggt agcagcagcg tgccgacgcg gtttgccgtc agattgcgcg aaaatcaagg
       61 aatctagagc ttgatccgat tcaattaaat cggatcaagc tctaaggcgc agcgatctcg
      121 ccgcgtatat gtgcgcgctc aatatgccgg ggtcttaccg ggcttgcttc aaccggcaat
      181 cccttcgatg aacgcccgca ggttggcggt ggagacggcg gggggcatgc cgccggcgca
      241 ggacggcagc atgcgcgaat ggtccgtggt ttccgccagc atcctgcgca cggcgtcgcg
      301 cacgccgtcg ggcgtgcctt gcgccagcac gtcgcgcggc ggaatattgc ccagaatcgt
      361 cagccggttg ccggtcacgc ggtgaagctc gttcatcgtc atgtgaatgc cgggattgta
      421 aaggttgatg ccgagatcgg gatagtgctt gaccgactgg cggcaatcgg cgtcgttgtg
      481 aaagagcttc accgccaccg gcgcgtcgga cagctctttc aggtacggcg ccgcgaactc
      541 caggtaatcc ttctcgctga cgaaaccgac gatgtcgtcg agcaccagca tgccgtcgat
      601 cgagtcgacg gcctggcgct ggcgggcgtg ccaatccatc aggaaggtgt tgatgacgcg
      661 cagcagcttg tgggtccgct cggggtcggt gccgagcatc atcaggaatt ccgtcgtgcc
      721 catcaggaag ctggcgatgt tgagcggccc gcgcgaaacc gagaatttga tttggtgccc
      781 catcgcttcg atgcgcgaac ggttgcgggt cagccggttg agcatgaacg gcagcaggcc
      841 gtcgatctcc acgttgggct gaacgatgtt atcgacatcc tcttcgctca ggacgttctt
      901 ctcggcgaag ggaaattcgt tctcgggaaa ctggcatttg cagccgaacg ccgacggctc
      961 ggtgcacatc ccgaattccg accagaaccc cggcaggaat atggcttcgg gaaaagtatc
     1021 gatggctttc ttgttggccg cgaaccacac ctcgtcgctc tggaagtatt cgaggatgtt
     1081 cacgccgtac cagttcggca gccaggggca gtcgataatg aacgccaccg gcagcggtcg
     1141 cttttgttcg ccgcgtatcg ccgcgagcaa ttcgttccat tgttgttcag tcatgacgtt
     1201 ccccccttgc tggcctgcgg ccttttcagc agattgccct taacgcggcc agcatacaac
     1261 cgcggccata caggcccgca gcgccgcggc ggccttagcc atgcgacatg tcgtgcattg
     1321 tccgcgccat gatggcatgc tgagactcga cgccgcagga cctccgcgga cggcgcggat
     1381 cagggggaaa acatggctca tcctctcatc caggaattaa gccgccgggc gccggtgatc
     1441 accgacggcg cctggggcac gcagcttcag gcgcgcggcc tggaaccggg cgaagtgccg
     1501 gacctgtgga atctgagcca tcccaaagaa gtcgccgcgg tcgcccgcgc ctatgtcgaa
     1561 gccgggtcgc agatcatcct gaccaacaca ttcggggcga accgctttcg cctggccgag
     1621 agcgacggcg ccggccgcct ggccgagatc aacaaggcgg gcgtcgccct gtcgcgccgc
     1681 gcggccgaca agaaggcgct cgtcttcgcc tcgctggggc cgagcggcaa gttgatgatg
     1741 aacgaggaag tcagcgaaga ggagttgcgc gccgccttcg ccgagcaggc cgaggccttg
     1801 gccgcagccg gaccggacgg aatcgtcgtc gaaaccatgc aggatttggc ggaagcgaaa
     1861 atcgctaccg aaacggcaaa agcaaccgga ctgccggtgg tcgtctgcat ggtgttcgat
     1921 tcgggcaagg acaaagaccg caccatgatg ggaaccacgc ccgaacaggc ggcggccggc
     1981 ctgatcgagg ccggggccga tgccgtcggc gccaattgcg gccagggtat cgccgggttc
     2041 gtgaacatct gcgcccggct gagagcggcg gcggaaggcc atccggtctg gatcaaggcc
     2101 aatgccggcc tgccggaaat acgggacgga aagacggtct acgaaatgac ggcggcacag
     2161 ttcgcatcct atgtccctgc cgtgctgacc gccggggcca gcttcatcgg cggctgctgc
     2221 ggaaccactc cggagttcat ccgcgacatc catcgcctgc ttcgcggtcc ctgatggacc
     2281 ggctgcggct gcatgtcatt gcctgttcca gcctgcggcc ggaactggaa atgctcgccg
     2341 cgcaagccaa gaccgccgtc actttgcggc atctggaaat ggggctgcac cagcgctccg
     2401 ccgaaacgct tcgcgccgtg ctgcaaggcg cgatcgatgc cgagacgatc gataatcgat
     2461 gcgatgccgt cgcactcggc tacggtctct gcaatcaggg cattgtcggg gtgcaggcgc
     2521 ggaggatgcc ggtggtcatt ccgcgggcac atgactgcat cggcatgctg ctgggcggca
     2581 gccggcaata tctcgcccaa ctggaggcgc agcccggcac ctatttccag agtgcgggat
     2641 ggctggagaa tgcgccggcc gagggcgacg tgcgccagcc ggacttcacc ttcggcccca
     2701 attccaacgt gacgcgcgag cgcctcgccg aacgctacgg cgacgacaac gccgattacc
     2761 tgctcgaaca attcgacggc ttcacccggc attacgaacg gctcgcctac atcgccacgc
     2821 cggcgcccgc ctcggcccgg tgggaagacg cggccaagga gaccgccgcc aaacgcaact
     2881 tgaccttcga gcggctcaaa ggcgatctcg gctggctcag gcggctcgtg aacggcgagt
     2941 ggaatgcaaa cgagtttctg acgctgcagc cgggcgagcg cgtcgtcttg accttcgacg
     3001 aacggctgat cggcgcggtg ccggcatgac caaaccgacc tgcgagcttg cgctttcgcc
     3061 ggccggcagg cggctgaaag tcgccgcggg catggcgctg cgcgatgtcc tgttcgaaca
     3121 gggcgtcgaa tttccctgcg gcggccacgg ccgctgcaaa ggctgccgtg tcaggctctt
     3181 gaaaggccac ctcgagccgg gtgccgagga agccagggtt ctgacgcaag ccgagcttgc
     3241 cgacggctgg cgcctggcct gccgccattt catcgccggc gacctggaac tggaactggc
     3301 gcaatgggaa atgcccatcc tgagcgacca ttccgctttc gcgttcacgc cgcgggcggg
     3361 cctcggcgcg gccgtcgatc tgggcaccac cacgatcgcc gcgcagcttc tcgatctcgc
     3421 aaccggcaat gtgctggccg cccgcacggc gctgaacgcg caagcccgcc acggcggcga
     3481 cgtcatcagc cgcgtcgaat acgccctgcg cggcggacaa ggcgaactcg aacggctggt
     3541 gcgcgaacaa atcgggcgga tgatcgggca attgctggat tcgacgccgc cggaacaaga
     3601 gctggtccgc atcgtcctcg tcggcaacac ggtgatgcac cacctgttct gcggcctcga
     3661 cgtgaccccg ctggcgacgc atccgttcga gccggagcat ccgggaagaa tccggttctc
     3721 caccgcccag ttgggctggg gcggaaacac ggccgtggag ttcatgccgt gcatcggcgg
     3781 cctggtcggc tccgatatcc tggcgggcat tgtcgcgacc ggtctcggtc aacgcgacgg
     3841 cttgggcgcc ctcatcgatc tgggcaccaa cggcgaagtc gtcgtgggcg gccgcgacag
     3901 gctcgtttgc acctcgaccg cggcggggcc ggcgttcgag ggcgcccgca tcgccatggg
     3961 catgcgcgcg gccaccggcg ccatcagcga ggtgcgggcg atagacggcg ccatcgtctg
     4021 ccgcatcatc ggcggtggtg cggcgcgcgg cttgtgcggc agcggcttgg tggacgccgt
     4081 ggcggcggga ttgaacctgg gctggatcga ggcgggcggg cggatgacgc tcggatcgat
     4141 gccgctatcc ggcccggtgg cgctgatccc ccaagacgtg cgcgaactgc aactggccaa
     4201 gggtgcgatt gccgccggcc tcaggatgct cgcggcccgg ctgggcgtgg cgccgggcga
     4261 gatcgggcag cttcatctcg ccggagcgtt cggcaatacc gtcggccgcg tcagcgcacg
     4321 gcggatcggc ctgctgcgtt tgcccgtcga gcggatcgtt cccgccggca acgccgccct
     4381 gctcggcgcc aagcgcgccc tgttcgaaga cccggttttg tgggacggcc tcgcgaagcg
     4441 catcgaacat gtggcgctca acgaagagcc ggcattccag gacatcttcg ccgaggaaat
     4501 gcggtttccg gagcgggcgt agggtggctt ggctgctcag gatgcgggct gcccaaatga
     4561 cgcaggcatg ccgccgaagg agtttcccgc gattcttctt tgatagcctc ggcaggtttc
     4621 atctggatgg cccgcattcg cgggccatga cagagagggg aataattcta gttatcccga
     4681 tcaggcataa aggccccgtg cgtcagccac tgcgctggcc gcgctgtggc tgtagccgtc
     4741 ggcgccgatc tccttggcgt aggcttcgct gatcggcgcg ccgccgacca tcactttcac
     4801 ctgatcgcga accccggcct tcttcaaggc ctcgatggtg gttttcatgg cgggcatcgt
     4861 tgtggtcagc agcgccgaca tcgcaacgat ctgcgcgccc ttgtccttca cggcggcgac
     4921 gaatttcgcc gggtccacgt tgacgccaag gtcgatgatc tcgtagccgc cgccttcgag
     4981 catggcggcc accagattct tgccgatgtc gtgaaggtcg cccttgacgg tgccgatggc
     5041 gatgcgcccg acgggttcga cgccgccggc ggccagcaac gggcggatca gctccaagcc
     5101 tgccttcatg gcgcgcgcgg cgagcagaag ctccggcacg aaatattcgt tgcactcgaa
     5161 gcggcggccc acctcgtcca tcgccggcat catggtttcc tgaacgaccg ccagcggcgc
     5221 gaccccggcg gcaagagcgg cctcggtggc ggcctttgcc gccggtacat ccccggtgag
     5281 gatcgcttcg tgcaacgatt tgacgtcgga catgggattc cctggtttat gccgttcaat
     5341 ttattatgtg gcgggcgggg caatatagca gcccgccgcc tgcgcgcaca ggctcccaac
     5401 ccaatcgaat tgcctcgccc cgagcctgtc gagaggcgag gcatgcaggt gacgcgatcc
     5461 cgtccgataa accgcccgac tatttgtccg tcttggtcca gcggtcgagc cagcccagga
     5521 cttcggcgta ccattgcgcc gagttctggg gcttgagcac ccagtggttc tcgtcgggga
     5581 aatagaggaa cttgctcgcg atgccgcggc gttgcagagc cgtgaaggtg ccaagcgctt
     5641 gcgtcagcgg aatgcggtag tcgcggctgc catggatcac cagcatcggc ttcgaccagg
     5701 atttgacgtg gttgtaggga ttgaatttct cggccgcttc gcgccactcg tagaacggcc
     5761 cgccttgttc ccattcgtcg aaccataatt cctcggtcgc atagcccatg ccgcgggtgt
     5821 cgaaggtgcc gtcgtgattg accaggcact tccacggctc gttccagacc ccggcgaccc
     5881 agttgaccat gaagccgcca tacgatccgc ccagcgcgca ggcgcggttg ccgtcgagga
     5941 aatcgtactt cgacaacgcg aaagcccagc ccttctgcaa atcttccagc gggcggtcgc
     6001 cccaatgacg gctgatggaa tcggtgaagg cctggccgta gccggtcgag ccgtggaaat
     6061 cgaccatcac caccgcgtag ccggcgttgg tatagatctg cggattccag cgatagctcc
     6121 aggcgttccc gaacgagcct tgcggcccgc catggatcag gaacgcgacc ggatatttca
     6181 ggccaggctt cggatcggcc ggcgggatca cgtagccgtg gaccgtctca tcgttccagc
     6241 ccttgaaggc gaactgttcg ggctcgcccc aatgaacgtc tttcagggcc tcgccgttga
     6301 tattcgtcaa cttgaccggc gccccgccgt ccaacccgac ccaaaacaac tgggcgggac
     6361 ccgtcaggct gtcgcgcgcg aagacgatgc cgtccggccc cacgtcgaag ccgccgacac
     6421 ggccctcgcc ggtcagcggc ttgaccttgc cgctcgcgac atcgatggcg aacaacttgc
     6481 tctggcccat ctcctcggcg gtggcgtaca gcgtcccgcc gtcgggcgac caggcgaggc
     6541 tccccggcga gcggtcccag gcgggggcga cttcgcgcac ggcgcccgtc gtcaggtcgt
     6601 gcagcatgat cgcgaaacgg tcggcttcga aacccggacg cttcatggcg cgataggcgg
     6661 cccatttgcc gtcggccgag aacaccggcg cggcatccca cgcctggttg gcctcggtgg
     6721 tgtcgcgcgg cggcgcggaa ccatcgagcg gcgcttccca gaggtcgaaa ttggtggtcc
     6781 acgcctcgtc tcttcccgac ttggccgaga acaccaccag cttgccgtcc ggcgcgatgg
     6841 cgaaatcctc gtcgccgccg aacggcttgg acggcacgtc gccgtcaaag ccggtcatca
     6901 gcggaaccgg gcgggcgctc ttggtgttca aggccaatgc gaagagatgg ttgcgggttc
     6961 cgtccgccca ggtatcccaa tggcgcacga gcaagcggtc atagaccacg ccagtcgcct
     7021 tgccggtctt cgtggcatcg agacgcttct tggtgcaggc cagatccgga cagtcgggaa
     7081 acacggcgag cgagatcacc aaaaccttgc catcgggcgc gagcttgaag gcgccgaccg
     7141 aaagcggcac ggaagtcacc tgcacggcct tcctgcccgc aaggtcggtg cggaacacct
     7201 gatcgatgcc cccggcgcgg ccggaaagga aatagatgct gccgtccggc gaccagcggc
     7261 cgctggtggc gccgccgtcg gaaatcttca gccggcggga ggcatgggtc ttcaggtcga
     7321 gcacccacag cgaccttgcg gccttgttgg cggcataatc gacggtgcgc agcgcgtaga
     7381 gaacgtaacg gccgtcgggc gacaggcgcg gatcgtcgat ccgctccagg ctcgccatga
     7441 gcttggcggt aaacggcacg gcggccgacg cgggcgcgag ggcggctgcc gccaacaaaa
     7501 gagcaaaggc ggatgcggca aggcggagca ttctggtcat aactggctgg gttccgaaag
     7561 ttggcgtttg ttcaatggag catattagcc ggtcgccgtt caaatcggca cgctgtctct
     7621 tttagagccg ttttggattg gaccagtgcg gccccgcccg gaacaacctt cgctagagag
     7681 caatccggca aagtgtgaag cggtttgccg ttagattgcg cgaaaaacat gaaatcgaga
     7741 gtgtgatccg attcaattaa atcggatcac gctctagtgt cccgattccg aaattcgcat
     7801 cctctctata tcggcttcgg gcgaatttcg aaaccacgac actagcgtag gcctgccgtc
     7861 atctgcgccc cgaatttcgc aatcaagagt cgcaattgcg gttcctccac cgtagcggcc
     7921 gcctcggcga cggtgcacca gcggcgcacg cgtccgccct tctccggcca ggtcttccgt
     7981 tcgcgcgtga ccttcagcgc aaagacttcg acccggcacg gcaggttgac gcctttcttg
     8041 atctgcttga aatattgaaa ttgaccgatg gcggtctttt ccatatcgcc gctgacgccg
     8101 gcctcttccc acgcttcgag cgcggcacag gcgaacggcc ggcgtccgtc gatcggccag
     8161 cccttgggaa tgatccagcg ccgcgtccgc agcgaggtca cgagcaggat ttcgagggtc
     8221 ccgccggaga cgcgataggg cagcgcggca tattgaatgc cggtgcttct cttgaccgct
     8281 accgcgcgcg gggatgccac gattgctctc accctagaac tccaaacaga tagaaagaga
     8341 cggcaccaat gatgccggcc gccggcatcg taacaaacca agctataaca atgtccttag
     8401 cgacgttcca gcgaaccgcg gaagtccgcc tggcggcacc gactcccacg atggcgcccg
     8461 tgatagtgtg ggtcgtcgag acgggaatgc caagcccggt cgccatgaaa agcgtgatgg
     8521 acccggccgt ctcggcgcaa gcgccttgca gcggcgccag ccgggtgatg cgggagccca
     8581 tggtgtgcac gatccgccag ccgccgcaca gagtgccgag cgccatcgcc gcctgacaac
     8641 tcaacaccac ccagaaggga acgtaaaaat cccccttgag cgcgccatgc gcgaagagca
     8701 gcgccgcgat gatgcccatc gtcttctgcg cgtcgttgcc gccgtgccca agcgaataca
     8761 aagacgccga aacgaactgc acctttcgga agatgccgtc cacgcgcgcc ggcgtgacgc
     8821 gcataaacga ccacgaaacc cccagtatca gcatcagcgc gagcacgaac ccgagccccg
     8881 gcgacaggaa aatcgcgccg atggttttgc ccaggccgcc ccactcgatc acgctccaac
     8941 ccgaacgggc tacccccgct cccaccagac cgccgatcag cgcgtgcgaa ctcgaagacg
     9001 gaatgccggc cagccaggtg acgacgttcc agacgatcgc gcccatcagc gcgccgaaga
     9061 tcacctggtc ggttatgata tcgggcgtca cgatgccggt gccgatggtt cgcgccacgt
     9121 gcatgccgaa aaccagaaag gcgatgaagt tgaagaacgc ggcccaggcc accgcatagc
     9181 gcggcgcaag gacccgcgtg gacacgatgg tggcgatcga gtttgccgcg tcgtgcagac
     9241 cgttcagaaa atcgaacagc agggcggcgg cgatcaacgc aatcaacagc ggctcagaca
     9301 ccgacgatgc tcccgcctaa gcctgttcga tgacaatgcc gctgatgcag ttggccacgt
     9361 cttcgaagcg atccatcacc ttttccagat ggtcgtagat ctcggtgccg acgatgaact
     9421 ccatcgcgct gccgccgcgg tgcttgacga aaagcacctt gatgccctga ttgtgcaatt
     9481 cgtccgcggc ttcttcgagc cggatgatct ctccggtaat gccgatcaac cgcgaagcct
     9541 gctggcgatg ggcgccgagc agggcaacgg catcgagcgt cagctttgcc gcctgcacga
     9601 tgatgtcgcc catatcggcc atctgccgct cgaagaccga tacctcgaac agcaggatcg
     9661 ctttcgcggt cttctgcatt tggtcgatcg aatcgtcgag cgaggaaatc agctctttga
     9721 tgtcgctgcg gtcgaacggc gtgatgaagg agcgccgcgt caggatcagc acgtcgcggg
     9781 tgatcgcgtc ggcctggttc tcgaaatcca ttaccttgcg gcaggcctcc ggcaccgtcg
     9841 ccccgtcatg cagaagggcg ttgagcgcct ccgaaccgtt cacgatggtg cgcgcatgcc
     9901 ggttgaagtg gtcgaagaac cgctcttcct tgggcatgag ggcctggagc cacagcatgg
     9961 acgatctcgt tccgaatcct cgcctcagtc ctgccagaga aggctccgcg gggtccacta
    10021 atattggtcg cggcgacctg aacgaacatc acgaaagcca tacgatccac aggttccgga
    10081 acagcccgta tgagttgcat cggtccgaag cgttctgcgg gcggtatctg cggctgcctc
    10141 gcgatccccc gggcagccgg cgaccgtttt gcgctgcaat ggcacaattg tcgcaagaaa
    10201 aaacaccggc tgtagccgca aaatgcaacc taagcgcttg acagataata gcctattaat
    10261 atgataattt tttgcgtgat gttgcccctt cacggcaaag ggcgacgcgc ggtgcgggac
    10321 tgtatccatg gcgctgatac cgtgcgttag aagctgcgat tttcagaccc tgccgcggcg
    10381 cgccgcacgg ccaccgacag gcgggaccgg ctgggaaccc gacgccggat cgttcccggc
    10441 ggatattctc ccggaagcgg cggcccgggc tttgagagag gcaagagtga aggacgcccc
    10501 atgagcacgt tcaccgccaa ggcgcccgcg cgcgcgaaca tcatcggtga acataccgat
    10561 tataccacca accagggcca cgtcctgccg acaccgctgt ctttccatac gacggtcggc
    10621 gtacaggaga ttgccggcgc ggccggccgg atgaccattt cgagcgagaa ctacggcaac
    10681 cagccgttcg agcgcagcac gcgcgacgaa ccgcggcgcc attggaccga ttacgtcgtc
    10741 ggctgcgtgc gcgtggtggc gaagaagacg gaattgcccg cgctcgagat caagatcaaa
    10801 agcgacgtgc cgatcggcag cggcatttcg agttcggcgg cgttgtgcgt cgcggttgtc
    10861 cgcgccttga aggggctgag cgggatcgag ctgagcggcg agcaaatcgc gctcatggcg
    10921 caccaggccg aacacgacta tgtcggcgtt ccttgcggca agatggacca gttcgtcgcg
    10981 tccctgggcc gccccggcga agccctgctg ctgaacacga ggaatttggc ctatcgccac
    11041 tatcccctgg ctgcgaacac ggcgatcatg ctggtcgatt gcggcaagaa gcacgacctc
    11101 gccaacggca gcggctacaa gcagcgcgtg gcggaatgca ctgaggcgcg ccatcgtttg
    11161 ggcgtgacca cgctgtgcga actgagctac gcggatctgg accgccttgc ggccctgccc
    11221 gacctgatcc ggcgccgcgc gcggcacgtg atcagcgaga atctgcgcgt gctggaattc
    11281 gtttccgcgc tggaaaaggg cgacctggag aagcaggcgg accttcttgc cgcgagccat
    11341 gtctcccagc gcgacgattt cgaggtctcg atcccggaga tcgacgccct ggtcgaagcc
    11401 tcgatccgct tcggcgtccg gggcgcgcgc atcgtcgggg gcggcttcgg cggcgccatc
    11461 atcgcgctgg tgccgcgcga gcaggcggtg gcatggtggt ctttcgtggc gaaggaaaat
    11521 cccaacagcc agttgatctc cacctacgcc ttgaagaaca gcgaaaacga gtacgccgcc
    11581 tgatcccgcc gccattctcg ttaccgcccg gcggagacgc atcttcgcac gatcaaatcc
    11641 gtgaaatcca cgaattatca ttacgataca tgtacttaga gtgtgcccgc ccatcttttt
    11701 aaggacacag gccgggtcgc gcacaaggcg tctgggg
//
LOCUS       DASWPU010000118         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_13120,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000118 DASWPU010000000
VERSION     DASWPU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13120"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="VGC27_02695"
     CDS             <1..1199
                     /locus_tag="VGC27_02695"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HEY0281514.1"
                     /translation="RAAHQRPANTRRGALERLFTLMFQGFVYNQIWEDPDVDLKALAL
                     QPHHRLITIASGGCNVLNYLAADPAKIIAVDLNPNHVALTRLKLAALANLPSYDEFFR
                     FFGQANDKANRKAYDYFLAERLDHVTRRYWEKHIPLHGRRINMFARNLYHYGLLGRFI
                     GILHVVAKLHGKKLEHILLARTLAEQRVAFERLIAPLFDHRSIRLLSKSPVSLYALGI
                     PPAQYDELVSATGGNAIAVLRERVERLACDFPIHDNYFAWQAFGRGYDLTNREAVPAY
                     LRREVYEIIRTRTDRVEVHHASVTDFLKHQDAHSLHRFVLLDAQDWMNPAQLASLWAE
                     IDRTADAADARVIFRTAGQDSPLPRKLPPELLTPWRYLEAESRAFHTKDRSSIYGGFH
                     VYARQP"
     gene            1207..1329
                     /locus_tag="VGC27_02700"
     CDS             1207..1329
                     /locus_tag="VGC27_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281515.1"
                     /translation="MSGAPVQNHAALMDEIYRHQRYVYDLTRKYYLFGRDRLIK"
     gene            1336..1860
                     /locus_tag="VGC27_02705"
     CDS             1336..1860
                     /locus_tag="VGC27_02705"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0281516.1"
                     /translation="MRPGDRLVELGCGTARNLIRIARRYPEAKLFGLDASRAMLETAS
                     EAVRRAGLETRIGLAHGYAEDLSPAMFGQDEAFDACVFSYSLSMIPDWKQALTAASAA
                     LSESGRIHIVDFGDLGGLGGAGRAILLAWLRLFHVTPRVELLHTFERNNSAALRVLPG
                     RYAFLLTDQRGNLF"
     gene            2047..3084
                     /locus_tag="VGC27_02710"
     CDS             2047..3084
                     /locus_tag="VGC27_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0281517.1"
                     /translation="MKLRSIALAGAAVLALSVPAAASDATGWYIGLGVGWDSIGDYTQ
                     ELTPASGPVVKYKTKAEDSALFIGSFGYRFDNRFRIENEIGYVSHDVKGGDLAFTGDV
                     SIKSDFVNLLYDYQLSPRWDLTFGGGIGAASVGLKTSYAGVRYMNKEKVRFGVQGIVG
                     INYSISANTDAGVDFRYRSFLGTTTCDCDLCTVSVGDLNEGAVMFNLRFFLQGPPPPP
                     PPPPPPPPPPPPPPPPPVTTYIVFFDFNKSNLTEAAQAVVTEAVKTAKTAGFVKVQVV
                     GHTDTVGSDTYNQALSERRAAAVKDEMIRQGLESDTIAIEGKSFHDPLVPTGPGVREP
                     QNRRAVIDLGK"
BASE COUNT          622 a         1038 c          943 g          641 t
ORIGIN      
        1 accgggccgc ccaccagcgc cccgccaata ccagaagagg tgccttggaa cgccttttta
       61 ccctgatgtt tcagggtttt gtttacaacc aaatctggga agacccggat gtcgatctca
      121 aggcgctggc gcttcagccg catcaccggc tgatcaccat cgcctcgggc ggctgcaacg
      181 tcctcaatta tctggccgcc gatccggcga agatcatcgc cgtcgatctc aaccccaacc
      241 atgtcgccct gacgcggctg aagctggcgg cgcttgccaa tcttccctct tacgacgaat
      301 tcttccgctt cttcgggcag gccaacgaca aggccaatcg caaggcctac gactatttcc
      361 tggccgagcg gctcgaccac gtcaccaggc gctattggga aaagcacatt ccgctgcacg
      421 gccggcgcat caacatgttc gcccgcaatc tctatcatta cgggttgctc ggacgcttca
      481 tcggcatcct gcacgtggtg gcgaaactgc acggcaagaa gctcgaacac atcctcttgg
      541 cgcgcaccct ggccgaacag cgcgtcgcct tcgaacgcct gatcgcgccg ctgttcgatc
      601 atcgctcgat ccgcctgttg tcgaaaagcc cggtgtcgct ctacgccctg ggcattccgc
      661 cggcgcaata cgacgaactg gtctccgcca ccggcggcaa cgccatcgcg gtgctgcgcg
      721 aacgggtcga gcgcctggcc tgcgattttc cgatccacga caattatttc gcctggcagg
      781 cgttcggccg cggctacgac ctgaccaacc gcgaagccgt cccggcctat ctgcgccgcg
      841 aggtttacga aataatccgt acccgcacgg acagggtgga ggtgcaccat gcctccgtca
      901 ccgacttcct caagcatcag gacgcccatt cgctgcaccg tttcgtgctg ctcgacgcgc
      961 aggactggat gaacccggct caactcgcct cgctatgggc cgaaatcgac cgcaccgccg
     1021 atgccgccga cgcccgcgtg atcttccgca ccgccggcca ggattcgccc ctgccgcgca
     1081 aactcccccc ggagttgctg acgccatggc gttacctgga agcggaaagc cgcgccttcc
     1141 acaccaagga tcgctcgtcg atctatggcg gcttccatgt ctatgcccgc cagccctgag
     1201 ccccccttga gcggcgcgcc ggtacaaaac catgccgcgc tgatggacga aatctatcgg
     1261 catcagcgct atgtctatga tctaacaaga aaatattatc tcttcggacg cgacaggctg
     1321 atcaagtagc tagctttgcg cccaggcgac cggctggtcg aactgggctg tggaacggct
     1381 cgcaatctca ttcgcatcgc gcggcgctac ccggaggcaa agctgttcgg gctggatgcc
     1441 tcccgggcca tgctggagac ggcctccgaa gcggtgcgcc gcgccggcct cgagacgcgc
     1501 atcggtctgg cacatggcta tgccgaggac ctctcgcccg cgatgttcgg ccaagacgaa
     1561 gccttcgacg cttgcgtctt ctcttatagc ttgtctatga ttccagattg gaaacaagca
     1621 cttacagcgg catcggcggc gttgtccgag agcgggcgca tccacatcgt ggatttcggc
     1681 gacctgggcg gactgggcgg cgccgggcgg gcgattctgc tcgcctggct gaggctgttc
     1741 catgtcaccc ccagggtgga gcttttacac acgttcgagc ggaacaattc ggccgcgctg
     1801 cgcgttctac cggggcgcta tgcatttcta ttgacggatc aaagagggaa tctcttctaa
     1861 gtttgccaac atgttgcaag gcggtaacag atacaggtca aatccaccgc agggcggtta
     1921 aggattatgg cctcgccata gacaagacca ttccggattt gccattatgg cttgcatacg
     1981 ttcgtggttc tgatgttccg gtggggggag acccgcccat cgattttctt gaagcgaggg
     2041 tggaaaatga aacttcgttc gatcgccttg gcgggcgctg cggtgctggc cctttccgtg
     2101 ccggcagccg cgagtgatgc aaccggctgg tatatcggtc tcggcgttgg ctgggacagc
     2161 ataggtgact acacgcagga attgaccccc gcctcgggtc cggtcgtgaa gtacaagaca
     2221 aaggcggaag attcggcgct gttcatcggc agcttcggct atcgcttcga caataggttc
     2281 cggatcgaaa acgagatcgg ctatgtcagc catgacgtca agggaggcga cttggccttc
     2341 acgggcgacg tcagcatcaa gtcggatttc gtcaacctgc tgtacgatta tcagctcagc
     2401 cccagatggg atctgacctt tggcggcggc atcggcgcag ccagcgtagg tctcaagacc
     2461 tcctacgcgg gcgtccgcta tatgaataag gaaaaggttc ggtttggcgt ccaaggaatc
     2521 gtcggcatca actattcgat tagcgccaac acggatgctg gcgtggactt ccgctatcgt
     2581 tcgttcctgg gcaccaccac ctgcgactgc gatctgtgca cggtgagtgt gggcgacctc
     2641 aacgaaggag cggtcatgtt caacttgcgg ttcttcctcc agggaccgcc gccgccgcct
     2701 ccgccgccac cgcctccccc gccgccaccg ccgcctcctc cgccgccgcc ggtgacgacg
     2761 tacatcgtct tcttcgactt caataagtcg aacctgacgg aagctgcgca ggccgtggtg
     2821 accgaagcgg tcaagacggc gaagaccgcc ggcttcgtga aggtacaggt cgtcggccac
     2881 accgatacgg tcggctccga cacgtacaat caggctctgt cagagcgccg cgctgcagcc
     2941 gtgaaggacg aaatgatccg tcaaggcttg gagagcgata cgatcgccat cgaaggcaag
     3001 agcttccacg atccgttggt cccgacgggc ccgggcgtgc gcgagcctca aaaccgcagg
     3061 gcggtcatcg atctgggtaa gtaagctcag cgacaaataa ggaaagggcg ggccggtcgg
     3121 cccgcccttt tcgttttcgg catgtgatcc gcggatcaaa tcccggagct agagcttcgt
     3181 acgttcagac gagatcacgt tgtcttcatg cttcgacccg cctacgctga agctccggcg
     3241 ggcc
//
LOCUS       DASWPU010000119         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_31512,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000119 DASWPU010000000
VERSION     DASWPU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31512"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2522)
                     /locus_tag="VGC27_02715"
     CDS             complement(<1..2522)
                     /locus_tag="VGC27_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEY0281518.1"
                     /translation="MSVVAESLLPPVFAQWFAARGWTPRAHQLALVEHAARGESVLLV
                     APTGGGKTLAGFLPSLIELAVPPLRFAARTTSPVNGGGKRPRRLHTLYISPLKALAVD
                     VARNLEAPVAEMGLDVRAETRTGDTPPSRRQRQKYSPPDILLTTPEQLALLLSHRDAP
                     RLFEHLKAVVLDELHAIHNSKRGDLLALGLARLAKLAPGHRRVGLSATVADPAPLQRY
                     LVPQPQGSEARAVLVRGAKGAVPEISILASEEYVPWAGHLARHAMGDVMAAIKRAKTA
                     LVFVNTRSQAERTFQDLWRINEDNLAIALHHGSLAPGQRRKVEAAMAGGRLRAVVCTS
                     TLDLGIDWGAVDLVICVGAPKGAARLVQRIGRANHRLDEPSRALLVPANRFEVLECEA
                     ARAAVIAGELDGERLRQGGLEVLAQHVLGMACSAPFDADALYTEVISASPYRALSRRD
                     FDQVVDFVATGGYALKRYDRYAKLRKTPEGLWRLAHPRLAQQYRLNAGTIIEEPMIDI
                     RLQARGRPTGVGRRPTGAGGRVLGRLEEYFIEQLSPGDTFVFAGEVLAFEGIREDAAF
                     VSRTQDHEAQVPSYMGGKFPLSTFLASRVREMVSKPETWNLLPDPVHEWLRIQEWRSV
                     LPQPGQLLAETFPRGQRFYLVCYPFEGRLAHQTLGMLLTRRLERAGMRPTGFSANEYA
                     LAVWGMRDMAGLDFGKLFDEDMLGDDLDAWLAESNLYKRTFRNCAIIAGLIERRSLHR
                     EKTGRQVTFSTDLIYDVLREHEPDHVLLRATYQDAATGLLDIARLGEMLKRVRGRIVK
                     RKLDRVSPLAVPALLEIGREMIVGEAGEDVLRETEQALIAEA"
     gene            2714..>3200
                     /locus_tag="VGC27_02720"
     CDS             2714..>3200
                     /locus_tag="VGC27_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY0281519.1"
                     /translation="MSNRREFLAATLAMAGASAFAGKAAAQTAPYRDATGMAPLPSNL
                     ADVRFPEDFFWGTATAAYQVEGAWNEDGKGESIWDRMAHTVGKIKGGDTGDVACDSYH
                     RYPEDIAIMTRLNQKSCRFSVAWTRIQPTGQGAVNRKGLDFYSRYVDALLEAGIRPVC
                     TL"
BASE COUNT          543 a         1129 c         1015 g          513 t
ORIGIN      
        1 atcgcctccg cgatcagcgc ctgttcggtt tcgcgcaaga cgtcctcgcc cgcttcgccg
       61 acgatcattt cgcggccgat ttccaagagc gccggcaccg ccagcggcga aacgcggtcg
      121 agcttgcgct tgacgatgcg cccgcgcacg cgcttcagca tctcgcccag ccgggcgatg
      181 tcgagcaggc cggtggcggc gtcctggtag gtcgcgcgca gcagaacatg gtccggctcg
      241 tgctcgcgca gcacatcgta gatcaggtcg gtggaaaagg tcacttgccg cccggtcttc
      301 tcgcggtgga gcgagcgccg ctcgatcagc ccggcgatga tcgcgcaatt gcggaaggtg
      361 cgcttgtaga ggttggattc cgccagccag gcgtcgagat cgtcgcccag catgtcctcg
      421 tcgaacagct tgccgaaatc gaggcccgcc atgtcgcgca tgccccacac cgccagcgcg
      481 tattcgttgg cggagaatcc cgtcggccgc atcccggccc gctccagccg ccgcgtcagc
      541 agcatcccca gcgtctgatg cgccaggcgt ccctcgaacg gatagcagac gagatagaag
      601 cgctgcccgc gcggaaaagt ctcggccagc aactgccccg gctgcggcag caccgagcgc
      661 cattcctgga tgcgcagcca ttcgtgcacc ggatcgggca gcaggttcca ggtttcgggc
      721 ttggacacca tctcgcgcac gcggctggcg aggaaggtgg aaagcggaaa cttcccgccc
      781 atgtaagacg gcacctgcgc ctcgtggtct tgggtgcgcg aaacgaacgc cgcgtcctcg
      841 cggatgcctt cgaaggccag cacttcgccc gcgaacacga aggtgtcgcc gggcgaaagc
      901 tgctcgatga aatattcctc gagccggccc agcacccgcc cgcccgcgcc ggtgggccgc
      961 ctgcccacgc cggtgggccg cccgcgtgct tgcaggcgga tgtcgatcat cggttcttcg
     1021 atgatcgtgc cggcgttcag ccggtattgc tgcgccaaac gcggatgggc aaggcgccat
     1081 aagccttccg gcgttttgcg cagcttggcg tagcggtcgt agcgcttgag cgcatagccg
     1141 ccggtcgcca cgaaatcgac cacctggtcg aaatcccggc gcgacagcgc ccggtacggc
     1201 gacgccgaaa taacctccgt atacagagca tccgcatcga acggcgcgct gcacgccatg
     1261 cccagcacat gctgcgccag cacctcgagc ccgccctggc gcagccgctc gccgtcgagt
     1321 tcgcccgcga tcaccgccgc gcgcgccgct tcgcattcga gcacctcgaa gcggttggcg
     1381 ggcaccaaga gcgcgcggct gggctcgtcg agccggtgat tggcgcggcc gatgcgctgc
     1441 accagacgcg ccgcgccctt gggcgcgccg acgcagatca ccagatcgac cgccccccag
     1501 tcgatgccga gatcgagggt ggaagtacac accaccgcgc gcagacgccc gcccgccatc
     1561 gccgcttcca ccttgcgccg ctggccggga gcgagcgagc cgtgatgcag cgcgatggcg
     1621 agattgtcct cgttgattcg ccacaggtcc tgaaaggtgc gctcggcctg gctgcgggtg
     1681 ttgacgaaga ccagcgccgt cttggcgcgc ttgatcgccg ccatcacgtc gcccatggcg
     1741 tggcgcgcca gatgccccgc ccagggcaca tactcttccg acgccagaat ggagatttcg
     1801 ggcaccgcgc ccttcgcgcc ccgcaccagc accgcgcgcg cttcgcttcc ctgcggttga
     1861 ggaaccaggt accgctgcaa cggcgcggga tcggcaaccg tcgccgaaag ccccacgcgg
     1921 cgatggccgg gcgccagctt cgccagccgc gccagcccca gcgccagcaa atcgccgcgc
     1981 ttggaattat ggatagcgtg gagttcgtcc agcaccacgg ccttcagatg ctcgaacagt
     2041 cgcggcgcat cgcgatgcga cagcagcagc gccaattgct ccggcgttgt aagaagaatg
     2101 tccggcggcg aatatttctg gcgctggcgg cgcgacggcg gcgtgtcgcc ggtgcgggtt
     2161 tcggcgcgca cgtccagccc catctccgcc accggcgctt ccagattgcg cgccacgtcc
     2221 accgccagcg ccttcagcgg cgagatatag agtgtatgaa gtctgcgagg tctctttcct
     2281 cccccgttta cgggggaggt ggttcgcgca gcgaaccgga gggggggcac tgccaactcg
     2341 atcaaactcg gcagaaaccc cgccagggtc ttgccgccgc cggtcggcgc caccagcagc
     2401 accgactctc cgcgtgccgc atgctccacc aacgcaagct gatgcgcccg cggcgtccat
     2461 ccgcgcgcgg cgaaccattg cgcgaagacg ggcggcagaa gcgattcggc aacaacgctc
     2521 atgcagcgac aatatcagat cggcggcctg atcctgcgtt cggccggcga acggaagcgg
     2581 cccgccgccc atatccttgc ccttcgtgcg ggtatttcga taggatatcc ttcgtcgatc
     2641 tctgcatttt atccgcaagg aattatcgtg ccccggcacc gtctttgttt tccaaacaga
     2701 aaaggagttc tccgtgagca atcgccgcga attcctcgcc gccaccctcg ccatggccgg
     2761 cgctagcgcc ttcgccggta aggcggccgc gcaaacggcg ccttaccgcg acgccacggg
     2821 catggccccg ctgccgtcga acctggccga tgtgcgtttc cccgaagact tcttctgggg
     2881 aacggcgacg gccgcttatc aggtggaagg cgcgtggaac gaggacggca agggcgaatc
     2941 catctgggac cgcatggcgc acaccgtcgg caagatcaaa ggcggcgata ccggcgatgt
     3001 cgcgtgcgac tcctatcacc gctaccccga agacatcgcc atcatgacgc ggcttaacca
     3061 gaagagctgc cgtttctccg tcgcctggac gcgcatccag cccaccggcc agggcgcggt
     3121 caaccggaag ggcctggatt tttacagccg ctatgtcgat gcgctgctgg aggccggcat
     3181 ccgccccgtc tgcacgctct
//
LOCUS       DASWPU010000120         9055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_37788,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000120 DASWPU010000000
VERSION     DASWPU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37788"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..2134)
                     /locus_tag="VGC27_02725"
     CDS             complement(152..2134)
                     /locus_tag="VGC27_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0281520.1"
                     /translation="MLRRLCLLSVAAAAVLPASAQEIYTPSAGGNIETVTVTASPMTK
                     SLDDTASIVAKVNRDDILQAGGGNIANALSSVPGVSSSSFAAGASRPIIRGMDSNRVK
                     LVENGLGSSDVSDIGPDHGVPIDPLSTQSIEVVRGAATLRYGSQAIGGVVNVLNNRVP
                     IALPDKPFALEATAAYGSVANSGDGALLADAAIGKFALHADAFYRNAGNYGTPDGTQA
                     NSFFTGDGFALGGSYFFGNNSHTGLGVVQYDAKYGIPSDDAFILMRQTKFISRSSFDW
                     GYGPLGAFNLDASYANYSHEEKNPDGSVNTTFKNKELDARGEQMIGAFGPFSSVAVGV
                     DVQNREYSAIGEDSSYLSPTVTTALGGYVFAEAPLTEALHLETSARIENVHISGTPAS
                     DASTARDFTPISAAAGLLYTATDWLKLGLTASTAGRAPAQTELFARGAHDGPGTFETG
                     DPALKIERANSLEGSARLRFQRFSFDLGVWSSWFHNYVYGELTGRTCDDDDACMPGDT
                     EELRELNYTQQGAHFRGLEGKGRYVLFDQGAGALSAEFQGDIVRATLNGGGNVPRIPP
                     WRLGGGLAWESGPFDAGVQVMVNGKQTQFGGFDTATPSYTELNASLAWRPVPGYEIAL
                     VGHNLTDAVERNAASFNKDVVELPGRDFRVVLRAAL"
     gene            complement(2205..2576)
                     /locus_tag="VGC27_02730"
     CDS             complement(2205..2576)
                     /locus_tag="VGC27_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281521.1"
                     /translation="MKAKVQVSAAGRKGSWRSLVAFLVLVAFSLQGFLIQTHIHELPA
                     SAPVTGVSVSVPLGGKTLPDTDTCLFCQEYVHAGAYLTSAAAAMLPPFAVVSLLAIFD
                     APLVAAPPLSHNWMGRAPPRA"
     gene            complement(2691..4151)
                     /locus_tag="VGC27_02735"
     CDS             complement(2691..4151)
                     /locus_tag="VGC27_02735"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEY0281522.1"
                     /translation="MNTHSASGDATATDLVFCATPEAAFDRLVLLYDEAIAEIEETFS
                     AFSRGEGGPAGEPPAYPYLSVDVEFGEMRSTGLAFGKVTSPGRYGTTITAPHLFRAYL
                     IEQLTLLTRKYEVAISVGKSRTPIPLTFAVERAASQMDAEQRFRLAERFPLPRLDAAH
                     DTIVDGGKWLGPGPAPLSLFSAERIDFSLHRLRHYSGTDPAHFQSFVLFTNYQRYIDE
                     FIVYCQTEIEEGRAERFVEPGNRITERGKEPSTPPLTKLPQMPAYHLVQPGNDGITLV
                     NIGVGPSNAKTVTDHLAVLRPHVWLMVGHCGGLRADQRLGDYVLAHAYLRDDQVLDDM
                     LPAEIPVPALAEVQVALAQAVSEVTGAEGRVLKERLRTGTVVTTGDRNWELRFAEQAK
                     RFNQSRAIAIDMESATIAANGYRFRVPYGTLLCVSDRPLHGEIKLPGMADTFYEERVG
                     QHLQIGIRALELLRKEAYAGTLHSRKLRSFTEPAFR"
     gene            complement(4156..5364)
                     /locus_tag="VGC27_02740"
     CDS             complement(4156..5364)
                     /locus_tag="VGC27_02740"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEY0281523.1"
                     /translation="MRAIIAVLDSFGLGSAPDAARFGDAGANTFGHIAKACAGGTIVR
                     GGRSGPLEIPNLLSLGLGLAAHEADPTIPSMQADKIIGRYGSAAERSKGKDTPSGHWE
                     MMGLPVETDWGYFPRTVPAFPAALTGALIARAKLPGILGNCHASGTEIIEKYGTEHIK
                     TGKPICYTSADSVFQIAAHESHFGLERLYEVCRIARKLVDEYNVGRVIARPFVGERPG
                     EFKRTGNRHDYATPPHRPTLLDIVKAQGREVVGIGKISDIFAGSGITKSLRADGNPRI
                     FDTMIACVKTAPDQSIVFANFVDFDQSFGHRRDVAGYAGALEAFDARIPELQTALKPG
                     DLAVFSADHGCDPTWTGTDHTREVVPILAFGPGIAPGAIGKRATFADIGQSIAGHLGL
                     DPLPVGTSFL"
     gene            5478..6314
                     /locus_tag="VGC27_02745"
     CDS             5478..6314
                     /locus_tag="VGC27_02745"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY0281524.1"
                     /translation="MSESPSELIERAAAAIRARLGEAFPKTAIVLGSGLGRFADKLSS
                     ARSMSYADIPGFSRSTVAGHHGNLMVGEVAGAPLAVMAGRIHAYEGHPPQAIAVPIRT
                     LRKLGVERLVLTNASGGLRADMPAGTLMIVADHINFSGTNPLAGPNDDSVGPRFPDMS
                     NAYDPQLRAMLADAACEAGVAVKPGVYLYTLGPNFETPAEVRMFASLGADAVGMSTVP
                     ECLAARHCGMKVAALSVVTNPAAGLSTVPLSHEETLAAAQSAYEKVERLLLKFFAKNT
                     TS"
     gene            6734..7387
                     /locus_tag="VGC27_02750"
     CDS             6734..7387
                     /locus_tag="VGC27_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0281525.1"
                     /translation="MLLTRKLFASACPIAILAAPLVLGVTPAFAQSTGSEQIETVIVT
                     GTKTVASAGLSSFSPVAKQNSSINQEFIKTQTPGQTFFQNLNMLPGINFTNSDPYGTS
                     GGNIRMHGQDGNHISVTLDGMPLNDTGNYAMYTNQMIDPELTERVTINQGTTDVDSPT
                     AAVTGGSIAVRTVRPNEDFGVLGKVSGGTHAFQRYFARVDSGEIGPWGTRAFVGASY"
     gene            7475..>9055
                     /locus_tag="VGC27_02755"
     CDS             7475..>9055
                     /locus_tag="VGC27_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0281526.1"
                     /translation="MNLAAHFNRNRNNSYFGPNITTNLVTNKAHWNSDYDATCTPATI
                     RPGVVDAVDNCGNFYKSRINPSDTGNIRFSSLWHLLDDLTLTVDSNLQYVLANGGGNT
                     TQAEHNAVMTGSYGYVSAASTAIVSPFGCIQASNGGCDLNGDGDILDTVRIYRPSTTN
                     TRRWGFNTSLIYAITEGHTIRAAYTLDYGLHRQTGQMALLGPDGSPYSVWAGLDDPEH
                     RIHTADGADLRYRDRKSKAILNQASMDYQGDFFDGMVQTSLGFRLPFFERDLNQYCYQ
                     IIRSTNPYCTSQIPSAVGPDNTVTFAGSSTKYIPPDSGVFRYNRFLPHLGISTKPFGD
                     EHQFFAAFTKELAAPRTDNLYASNGTIDSPTTAVDIYSAFDGATPETSTSYTVGYRFI
                     GESLNASLSAWNSQVKNRLISTWDQEAQISTYHSVPGINFAGIDADASYNVTDDLSVY
                     GSASFMNARLISDIVVGGTASAPIYAYTGGKTLAETPHWTFTGRVQYKPLPELRFGLG
                     GKYVGKRFATDTNNISVPD"
BASE COUNT         1671 a         2829 c         2945 g         1610 t
ORIGIN      
        1 gtctgcggcg ccgccccgcc gctgcttgcg aagacgagca gggacaagac gcaaaaaagc
       61 ggcaacgaac gcatgacggt cctccggtga acggcgcaac gcatcttagg cgccgcaccg
      121 gaggctgccg atcacgtttc tgtgcgcggc ctcagagggc cgcccgcagc accacgcgga
      181 agtcgcggcc gggcagttcc acgacgtcct tgttgaagga ggcggcgttg cgctccaccg
      241 cgtcggtcag gttatggccg accagagcga tctcgtagcc gggcaccggc cgccaggcga
      301 ggctggcgtt cagctcggtg taggaaggcg tggccgtgtc gaaaccgccg aactgcgtct
      361 gcttgccgtt gaccatcacc tgcacgcccg catcgaacgg accgctttcc caggccagcc
      421 cgccgcccag ccgccaaggc ggaatgcgcg ggacgttgcc gccgccgttc aaagtggcgc
      481 gcacgatgtc gccctggaat tccgccgaca gcgcgcccgc gccctggtcg aacagcacat
      541 agcggccttt gccttcgagg ccgcggaagt gcgcaccctg ctgcgtatag ttgagttcgc
      601 gcagttcctc cgtatcgccg ggcatacaag cgtcgtcatc gtcgcaagtg cggccggtca
      661 gttcgccata gacgtaattg tggaaccagg aactccagac gccgaggtcg aagctgaagc
      721 gctggaagcg cagccgcgcc gagccttcca gcgaattcgc ccgctcgatc ttcaaggccg
      781 gatcgccggt ctcgaaggtg cccggcccgt catgggcgcc gcgggcgaag agttcggtct
      841 gggccggagc gcgcccggcg gtggacgccg tcagccccag cttcagccag tcggtggcgg
      901 tgtagagcag ccccgccgcc gcgctgatgg gggtgaaatc gcgcgcggtg gaagcatccg
      961 aggcgggcgt gcccgagatg tgcacgttct cgatccgggc gctggtttcc aggtgcagcg
     1021 cttcggtcag cggcgcctcg gcgaagacgt agccgcccaa ggccgtggtc acggtcggcg
     1081 acagatagct gctgtcctcg ccgatggcag aatattcgcg gttctgcaca tcgacgccta
     1141 ccgcgacgga ggaaaaggga ccgaaagcgc cgatcatctg ttcgccgcgc gcatccagct
     1201 ccttgttctt gaaggtggtg ttgaccgaac cgtccgggtt cttctcctcg tgcgaatagt
     1261 tggcgtagga agcatcgagg ttgaaagcgc ccagcggccc gtaaccccaa tcgaaagagg
     1321 agcggctgat gaacttggtc tgccgcatca ggatgaaggc gtcgtcgctg gggatgccgt
     1381 atttggcgtc gtattgcacc acgcccagcc ccgtatggct gttgttgccg aagaaatagc
     1441 tgccgccgag cgcaaagccg tcgccggtga agaaggagtt ggcctgggtg ccgtccggcg
     1501 tgccgtaatt gcccgcgttg cgatagaagg cgtcggcatg gagggcgaat ttgccgatgg
     1561 cggcatccgc caacagcgcg ccgtcgccgg aattcgccac cgagccgtaa gcggccgtgg
     1621 cttccagggc aaagggcttg tcgggcaggg cgatgggcac gcggttgttg agcacgttga
     1681 ccacgccgcc gatggcctgg ctgccgtagc gcaaggtggc cgcgccgcgc accacttcga
     1741 tgctctgggt ggagagcgga tcgatgggca cgccgtgatc gggcccgatg tcggacacgt
     1801 cggaactgcc cagcccgttc tccaccagct tgacgcggtt ggaatccatg ccgcggatga
     1861 tgggccgcga agcccccgcc gcgaagctgg aagaggaaac gccgggcacg ctggaaagcg
     1921 cgttggcgat attgccgccg cccgcctgca ggatgtcgtc gcggttcacc ttggccacga
     1981 tgctggcggt gtcgtcgagc gatttggtca tgggcgaggc cgtgacggtg acggtctcga
     2041 tgttgccgcc tgcggacggg gtgtaaatct cttgggcgga cgcgggaaga actgctgccg
     2101 cggccacgga cagcagacaa agtctgcgaa gcataggaag tctccacgat tgatctggat
     2161 gccaaagagc ccgcggcgca cgccgcgagc atgggggcga aggctcaggc gcgtggaggc
     2221 gcgcggccca tccagttgtg cgaaagcggc ggtgccgcga ccagcggcgc gtcgaaaatg
     2281 gcgagcagcg acaccaccgc gaagggcggc agcatggcgg ctgcggccga cgtcagatac
     2341 gcgccggcgt gaacgtattc ctggcagaag aggcacgtgt ccgtgtccgg cagcgtcttg
     2401 ccgccaagcg gtaccgaaac cgacacgccc gtcaccggcg cggaggcggg cagctcatgg
     2461 atgtgggtct ggatcaggaa gccttgcagc gaaaaggcga caagcaccag gaacgcgacc
     2521 agggatcgcc atgatccctt ccgccctgcg gctgatacct gcactttcgc tttcatgcga
     2581 tcctcacgcg gccatctagg cagaaatgcg cgccccgcgc aacgcttacc gcggcggcaa
     2641 tgttgacgcg tttgccgttc atggctttag ggcaggaaag cggcggcgat ttacctgaac
     2701 gccggttcgg tgaagctgcg cagcttgcgc gagtgaagag ttccggcata ggcttccttg
     2761 cgcagcagtt ccagcgcgcg gatgccgatc tgcaaatgct ggccgacgcg ctcttcgtag
     2821 aacgtgtcgg ccatgccggg cagcttgatc tcgccgtgca gcggccggtc cgagacgcac
     2881 agcagcgtgc cgtagggcac gcggaaacgg tagccgttgg cggcgatggt cgccgactcc
     2941 atgtcgatgg cgatggcgcg cgactggttg aagcgcttgg cctgttcggc gaagcgcagt
     3001 tcccaattgc ggtcgccggt ggtgacgacg gtgccggtgc gcagccgttc cttcagcacc
     3061 cgcccttcgg cgccggtgac ttcgctcacc gcctgggcga gggccacttg cacttcggca
     3121 agcgccggca cggggatttc cgccggcagc atgtcgtcga gcacctgatc gtcgcgcaga
     3181 taggcgtgcg ccagcacgta atcgcccagg cgttgatcgg cgcgcagccc gccgcaatgg
     3241 ccgaccatca gccagacgtg cggacgcagc acggcgagat ggtcggtcac ggtcttggcg
     3301 ttcgacggcc cgacgccgat gttcaccagc gtgatgccgt cattgcccgg ctggacgagg
     3361 tgataggccg gcatctgcgg cagcttcgtc agcggcggcg tggacggttc cttgccgcgc
     3421 tcggtgatgc ggtttcccgg ttcgacgaag cgttcggcgc ggccttcctc gatctccgtc
     3481 tggcaataga cgatgaattc gtcgatgtag cgctgatagt tggtgaacag cacgaagctc
     3541 tggaaatgcg ccgggtcggt gcccgagtag tggcgcagcc ggtgcagcga gaagtcgatg
     3601 cgctcggccg agaacagcga cagcggcgcg ggccctgggc ccagccactt gccgccgtcc
     3661 acgatggtgt cgtgcgccgc gtcgaggcgg ggcaggggaa aacgctccgc cagccggaac
     3721 cgctgttcgg cgtccatttg cgaggcggca cgctcgaccg cgaaggtcag cggaatcggc
     3781 gtgcggctct tgccgacaga gatcgcgact tcgtacttcc gcgtcagcag cgtgagctgt
     3841 tcgatcagat aggcgcggaa cagatgcggg gcggtgatcg tggtgccgta gcggccgggg
     3901 ctggtgacct tgccgaaggc aagcccggtc gagcgcattt cgccgaactc tacatcgaca
     3961 ctgagatagg gataagccgg aggttcgccg gccggcccgc cctcaccgcg cgaaaaggcg
     4021 ctgaaagtct cttcgatttc cgcgatggcc tcgtcgtaaa gcagcaccag ccggtcgaag
     4081 gcggcctcgg gagtggcgca gaataccaag tcggtggcgg ttgcatcgcc ggaagcggag
     4141 tgtgtgttca tggcttcaca agaaagatgt tccgaccggg agcgggtcga ggcccagatg
     4201 gccggcgatg gattggccga tgtcggcgaa agtcgcgcgt ttgccgatgg cgcccggcgc
     4261 gataccggga ccgaaggcga ggatggggac gacttcgcgg gtgtgatcgg tgccggtcca
     4321 ggtcgggtcg cagccgtgat cggcggaaaa cacggcgaga tcgcccggct tcagcgccgt
     4381 ttgcagttcg gggatgcgcg cgtcgaaggc ttcgagcgcg ccggcatagc ccgccacgtc
     4441 gcgccggtgg ccgaaggatt ggtcgaaatc cacgaagttc gcgaagacga tcgactgatc
     4501 gggcgcggtt ttcacgcacg caatcattgt gtcgaagatt cgcgggttgc cgtcggccct
     4561 cagactcttg gtgatgccgc tgcccgcgaa gatgtctgag atcttgccga tgccgacgac
     4621 ttcgcggcct tgcgccttga cgatatcaag caaggtcggc cggtgcggcg gcgtggcgta
     4681 gtcgtggcgg ttgccggtgc gcttgaattc gccgggccgc tcgccgacga acgggcgcgc
     4741 gatcacccgc ccgacattat attcgtccac cagcttgcgg gcgatgcggc agacctcgta
     4801 gaggcgttcg agcccgaaat gactttcatg cgcggcgatc tggaacacgg aatcggccga
     4861 ggtgtagcaa atcggcttgc ccgtcttgat gtgttcggtg ccgtactttt ctatgatctc
     4921 ggtgcccgaa gcgtggcagt tgcccagaat gccgggcagc ttggcccgcg caatcaaggc
     4981 gccggtcagg gcggcgggga aggcgggtac ggtgcgcggg aaatagcccc agtcggtctc
     5041 gaccggcagg cccatcattt cccaatggcc gcttggcgta tccttgcctt tgctccgttc
     5101 cgcggcgctg ccataacggc cgatgatttt gtcagcctgc attgacggaa tagttggatc
     5161 ggcctcgtgc gcggccagcc ccagtcccag cgacagaaga ttcggaatct caagcggtcc
     5221 gctgcgaccg ccgcggacga tggttccacc ggcgcaggcc ttggcgatgt ggccgaaggt
     5281 gttggcgccc gcgtctccga agcgcgcggc gtccggcgcg gaaccgaggc cgaaggaatc
     5341 gaggactgct atgattgctc gcaatgaaag ccgctccgga tgctgccgcg gcaccctttt
     5401 tgtcattatg ccccggcatt gttccaagaa agcccggcct tgtccatgtg caccggcggc
     5461 tgctaacacc tgcgcccatg agcgaaagcc cctccgaatt gatcgaacgc gccgccgccg
     5521 cgattcgcgc gcggctgggc gaggcgtttc ccaagacggc catcgtgctc ggcagcggtc
     5581 tgggccgctt tgccgacaag ctgtccagcg ccaggagcat gtcctacgcc gacatccccg
     5641 gcttctcccg ttcgacggtg gcgggccatc acggcaacct gatggtcggt gaggtcgctg
     5701 gcgcgccgct cgccgtcatg gcggggcgca tccacgccta tgaagggcat ccgccgcagg
     5761 cgatcgccgt gcctattcgc accttgcgga aactcggcgt ggagcggctg gttctcacca
     5821 acgcttcggg cgggctgaga gcggacatgc cggcgggcac gctgatgatc gtcgcggacc
     5881 acatcaactt ttccggcacc aacccgctgg cgggcccgaa cgacgacagc gtcggcccgc
     5941 gctttcccga tatgtcgaac gcttacgatc cccagttgcg cgcgatgctc gccgatgccg
     6001 cgtgcgaagc cggcgtcgcg gtgaagccgg gcgtctatct ctacacgctg ggtcccaatt
     6061 tcgagacgcc ggccgaagtg cggatgttcg cttcgctggg agcggatgcg gtgggaatgt
     6121 ccaccgttcc cgaatgtctg gcggcccgtc attgcggaat gaaagtggcg gccttgtcgg
     6181 tggtgacgaa tcccgcggcc ggattatcaa cggttccttt gtcgcacgaa gagacgctcg
     6241 cagccgcgca aagcgcctat gaaaaagtgg agcggctgct actgaagttc tttgcgaaaa
     6301 acacaacgtc ctgaaaagcg ttgcgttgca gcagcgtggc ggccacaccg gggcatgttt
     6361 gcgaaaaagg ctttcttaaa ataattctta atctcataat tttttcttaa ggctcgcgct
     6421 gtgccctgaa aagcacgcgc ttgtcataaa agttaattgg ttattgacgt aaaaacggct
     6481 gtaacagcag acttttctgc aagtgcgaag aatatggatc tgcgcgcgtt gacgtaagac
     6541 cagtggggtt gaattgtcat gtgccgtgtg tgcggagctg tgcgtcgttc gcagcgatac
     6601 attttatcgt tgccggcgca gaccaatccc gttcgacgcc cttcatttga ctcgacggcg
     6661 accgaggcga cgcagcgatg ctgcgtgtgc ggtgccgcgt gaccaaacca gctgaaaaca
     6721 ggggaagaag acaatgcttt tgactaggaa attgtttgcg agcgcgtgcc cgatcgcgat
     6781 cctggcggcg ccgctcgtac tgggcgttac gccggccttc gcgcagtcga cgggttccga
     6841 acaaatcgaa actgtgattg tcaccggcac gaagaccgtt gccagcgccg ggttgagttc
     6901 gttcagtccc gtcgcaaagc agaattcttc gatcaaccag gaattcataa agacgcagac
     6961 gcccggtcaa acgttcttcc agaacctgaa catgctgccg ggcatcaact tcaccaactc
     7021 cgatccctat ggcacgtccg gcggcaacat ccgcatgcac ggccaggacg gcaaccacat
     7081 ctccgtcact ctggacggca tgccgctgaa cgacaccggc aactacgcga tgtacacgaa
     7141 ccagatgatc gatccggaac tcacggagcg ggtcaccatc aaccagggta cgaccgacgt
     7201 cgacagcccg accgcggccg tgaccggcgg ttcgatcgcg gtgcgcacgg tgcgtccgaa
     7261 cgaagatttc ggcgtcttgg gcaaggtgag cggcgggacg cacgcctttc agcgctactt
     7321 cgcccgcgtg gactccggcg agatcggccc ttggggcaca cgcgctttcg tcggcgcgtc
     7381 ctactagact tatgacaagt tcaagggccc gggcaagctg cagaagaagc agatcaacgc
     7441 cgacatccat caggaccttg gcgatctcgg ctggctgaac cttgcggccc acttcaaccg
     7501 caaccgcaac aactcgtatt tcggcccgaa tatcaccacg aatctcgtca cgaacaaggc
     7561 gcattggaac tccgactacg atgcgacctg cacccccgcc acgatccgac cgggtgtcgt
     7621 cgatgcagtc gacaattgcg gcaacttcta caagtctcgc atcaatccgt cggataccgg
     7681 caacattcgc ttctcgtcgc tctggcacct gttggacgat ctgacgctga cggtcgactc
     7741 gaacctgcaa tacgtcttgg cgaacggcgg cggcaacacg acccaagccg agcacaacgc
     7801 tgtgatgacc ggttcctacg gctatgtcag cgccgcttcg accgcgatcg tatccccgtt
     7861 cggctgcatc caggcgagca acggcggctg cgacctgaac ggcgacggcg atattctcga
     7921 cacggttcgc atctaccggc ccagcacgac caatacgcgc cgctggggct tcaacacatc
     7981 gttgatctat gcgatcaccg aaggccacac gatccgcgcc gcctacacgc tcgattacgg
     8041 tctgcatcgc cagaccgggc agatggcctt actggggccg gacggcagcc cctacagcgt
     8101 ctgggcgggc ctggacgatc cggagcatcg catccacacg gccgatgggg cagatttgcg
     8161 ctaccgcgac cgcaagtcca aggcgatcct gaaccaggct tccatggact atcagggcga
     8221 tttcttcgac ggcatggtgc agaccagtct cggcttccga ctgccgttct tcgaacgcga
     8281 cttgaaccag tactgctatc agatcatcag atcgaccaac ccgtactgca cgagccagat
     8341 tcccagcgcg gtggggccgg acaatacggt gacgttcgca ggcagttcga cgaagtacat
     8401 cccgccggac tcgggcgtgt tccgttacaa ccgcttcctg ccgcacctcg gtatctcgac
     8461 caagccgttc ggcgacgagc atcagttctt cgccgccttc accaaggaac tggcggcacc
     8521 gagaaccgac aacctgtacg cttcaaacgg cacgatcgac agcccgacca ccgccgtcga
     8581 catctactct gcgttcgacg gtgcgacgcc ggaaacttcc acgagctata cggtcggtta
     8641 tcgctttatc ggcgaaagcc tcaacgcatc gctctcggcg tggaacagcc aagtcaagaa
     8701 ccgcctgatc tccacctggg atcaggaagc ccagatcagc acctaccaca gcgtgccggg
     8761 catcaatttc gccggtatcg acgccgacgc cagctacaac gtcacggatg atctgtcggt
     8821 gtacggttcg gcctcgttca tgaacgcccg tctcatctcg gatatcgtgg tcggcggcac
     8881 cgcgagcgcc ccgatctacg cttacaccgg cggcaagact ttggcagaga cgccccattg
     8941 gacgtttacg ggccgcgtgc agtacaagcc gctgcccgag ctgaggttcg gtcttggcgg
     9001 aaagtatgtc ggcaaacgtt tcgccacgga tacgaacaac atcagcgttc ccgac
//
LOCUS       DASWPU010000121         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_13123,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000121 DASWPU010000000
VERSION     DASWPU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13123"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VGC27_02760"
     CDS             <1..575
                     /locus_tag="VGC27_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0281527.1"
                     /translation="TGLSGAGKTTLLKLIDIAEPPSRGLISLFGLDLATTKRSAFPAL
                     RRRIGVVFQDFRLLDHLSAFDNVALPLRIAGRRESDYGRDVEELLAWVGLGDRLKAKP
                     PTLSGGEQQRVAIARAVVAKPDLLIADEPTGNVDPEIGARLIRLFGELNRLGTTVLIA
                     THDRTLVEKTRAREMRLVDGRLVELRTPRI"
     gene            593..1462
                     /locus_tag="VGC27_02765"
     CDS             593..1462
                     /locus_tag="VGC27_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281528.1"
                     /translation="MPREKGAAPLDVVIAVMAFLAALALGASLVAERTALGWRAGLAG
                     KVTVQILPPAQGPASEGLQRETEAALKVLRTTAGIAHAAPLSEDEKLKLVQPWLGQGS
                     LVADLPLPQLIDAEIVPGAEVDLNGLAERLKQAAPNSLLDDHSHWIGRLRDLAQSVVW
                     TAYGILLLIAVATAAAVSFATRAGLEAHHEMVALLHQMGARAEFISRAFEWYYFRSSL
                     AAAAAGTAMAAIFFLGAGGLQGAGIEAVPFLPPLALQPIELPWLLAVPAAVGLIALAT
                     ARLSVLAALRDIY"
     gene            1735..2349
                     /locus_tag="VGC27_02770"
     CDS             1735..2349
                     /locus_tag="VGC27_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY0281529.1"
                     /translation="MLKWIAIAVAGLYAAGFVVFVNTLPKTPHDLRHIQGIVALTGGD
                     ARLDAAVALFEKGVGRRLLISGVNPQTSKTELKKLAHGGPRFYCCVDLGYAAEDTHGN
                     AREAGAWARFYHFRKLLIVTARYHMPRSLSEFQAAVPDMTIVPYPVDPVSIDMRGWWH
                     DPHALRVLHSEYAKFLAAMALTSIGNKPNSLDRGTRHGEARSPS"
     gene            2364..>2832
                     /locus_tag="VGC27_02775"
     CDS             2364..>2832
                     /locus_tag="VGC27_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0281530.1"
                     /translation="MTVFRSLLFFLWFAFVSVALHLAALPALLLPRRATVWASRCWSA
                     ALLWGLKAIVGLDYQVRGEIPKGGVLVAAKHMSMWDTVALYLLLFDPAVVVKRELLRI
                     PFYGWYIQKVRVIPIDRSGHASALRQMAAQGRAAIAAGRPIVIFPEGTRKKPDA"
BASE COUNT          468 a          908 c          918 g          538 t
ORIGIN      
        1 tgaccgggct ttccggcgcc ggcaagacga ccctgttgaa actgatcgat atcgccgaac
       61 cgccttcgcg cgggcttatc agcctgttcg gactcgacct ggcgacgacc aagcgttccg
      121 cctttccggc cttgcgccgc cgcatcggcg tggtgttcca ggatttccgc ctgctcgacc
      181 atctttccgc cttcgacaat gtggcgctgc ctttgcgcat cgccgggcgg cgggaatccg
      241 attacggccg cgatgttgaa gagctgctcg cctgggtggg gctgggcgac cggctgaagg
      301 ccaagccgcc gacgctgtcg ggcggcgagc agcagcgcgt cgccatcgct cgcgccgtgg
      361 tggccaaacc cgatctgctg atcgccgacg aaccgaccgg caatgtcgat cccgaaatcg
      421 gggcgcgcct gatccgcctg ttcggcgaat tgaaccggct gggcaccacc gtgctgatcg
      481 ccactcacga ccgcaccctg gtcgagaaga cccgcgcccg cgagatgcgg ctggtggacg
      541 gcaggctggt cgaattgagg acaccgcgca tatgagcgcc aaggaccgat tcatgccgcg
      601 cgaaaagggc gccgcgccgc tcgacgtggt gatcgcggtg atggcgtttc tggcggcgct
      661 ggcgctgggc gcttcgctgg tcgccgagcg cacagcgttg ggttggcgcg ccggtctggc
      721 ggggaaagtg acggtgcaaa tcctgccgcc cgcgcaaggc ccggccagcg aaggattgca
      781 gcgcgaaacc gaagcggcgc tcaaagtgct gcgcacgacc gccggcatcg cccatgccgc
      841 gcctctgtcg gaggatgaaa aactgaaact ggtccagccc tggctcggcc aaggcagttt
      901 ggtcgccgat ctgccgttgc cgcaattgat cgacgcggaa atcgtgcccg gcgcggaagt
      961 cgatctgaac gggctggcgg aacggctgaa acaagcggcc ccaaattcgc tgctggacga
     1021 tcacagccat tggatcggcc gcttgcgcga tctggcgcaa tcggtcgtct ggaccgccta
     1081 tggcatcctg ctcttgatcg cggtggcgac ggcggcggcg gtttccttcg cgacccgcgc
     1141 gggcctggaa gcccatcacg agatggtggc gctgctgcat cagatggggg cgcgcgccga
     1201 attcatctcc cgcgccttcg aatggtatta tttccgttcg tcgctggcgg cggcggcggc
     1261 aggcacggcc atggccgcga tcttcttcct gggcgcggga gggctgcaag gcgccggcat
     1321 cgaagccgtg cctttcctgc cgcccttggc gctgcaaccg atcgaattgc cttggctcct
     1381 cgccgttccg gcggcggtcg ggctgatcgc gctggccacg gcgcggctgt ccgttttggc
     1441 ggcgctcaga gacatttact gagccatcag accgcagccg cgcaattcgg ggatttttca
     1501 gcttttgcgc gccaagatgc gaggaaactc gtgttattct agtgtcgtgg tttcgaagtt
     1561 cgcagcatgc gcactatcgg gctggaagcg aacttcgaaa ccaaaacgac actaggattc
     1621 aataggttgc tagtgtcctt tcgattccga aattcgcccg aagccgatat agagagggtg
     1681 cgaatttcgg aaatcgggac actaggttga gcgcgggaac gaggaacctt tgcgttgctg
     1741 aaatggatcg ccatcgccgt cgccggcctc tatgccgcgg gcttcgtcgt cttcgtgaac
     1801 accctgccga agacgccgca tgatttgcgc catatccagg gtatcgtcgc gctgaccggc
     1861 ggcgatgcac gcctggacgc cgccgttgcc ctgttcgaaa aaggcgtcgg ccgaagactg
     1921 ctgatctcag gggtcaatcc gcaaacctcc aaaaccgagc tcaagaagct ggcccatggc
     1981 ggcccccgct tctattgttg cgtcgatctg ggctatgccg ccgaggatac ccatggcaat
     2041 gccagggaag caggcgcctg ggcgcgtttc tatcatttcc gcaagcttct catcgtcacg
     2101 gcgcgctacc acatgcccag aagcctctcc gagttccagg ccgccgtgcc ggacatgacg
     2161 atcgtgccct acccggtcga tccggtgagc atcgatatgc gtggctggtg gcacgatccg
     2221 cacgcgctgc gcgtgctgca cagcgaatat gcgaaatttc tggccgcgat ggcgctgacg
     2281 tccatcggca acaagcccaa tagccttgac cgcggcacac ggcatggcga ggctcgcagc
     2341 ccgtcgtgat catgccggat gccatgaccg tctttcgctc gctgctgttc tttctctggt
     2401 tcgctttcgt ctcggtggcg ctgcacctcg ccgcgctgcc cgccctgctg ctgccgcggc
     2461 gcgcgacggt gtgggcgtcg cggtgctgga gcgcagcctt gctgtggggc ctcaaggcga
     2521 ttgtcggcct cgattaccaa gtgcgcggcg aaattcccaa aggcggcgtg ctggtggccg
     2581 ccaagcatat gagcatgtgg gacacggtgg cgctctatct gctgcttttc gatcccgccg
     2641 tggtggtcaa gcgcgaactg ctgcgtattc ccttctacgg ctggtacatc caaaaagtcc
     2701 gcgtgattcc catcgaccgc agcggccatg ccagcgccct gcgccagatg gcggcgcaag
     2761 ggcgcgccgc gatcgcggcg gggcggccca tcgtcatctt tcccgaaggc acccgcaaaa
     2821 agcccgatgc cg
//
LOCUS       DASWPU010000122         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_19323,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000122 DASWPU010000000
VERSION     DASWPU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19323"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VGC27_02780"
     CDS             complement(<1..771)
                     /locus_tag="VGC27_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEY0281531.1"
                     /translation="MHPLSQPLTLPCGAVLSNRLCKTAMSEGMADVDNHSTPRLETLY
                     RRWATSGAGLLFTGNIQVDPDHLERPLNLVIRDGAGKEQLAKLAAAGKSGGAHFWAQL
                     SHTGRQVDAAFNPAPLAPSAVELDVIRGAGFNFAEPRAMTESQIERAIGQFGFAARQV
                     REAGFTGVTLHAAHGYLISQFLSPLSNRRKDKWGGSLENRARFLMAVLAAVREAVGPG
                     FPIGAKLNSSDFQRGGFTSRECIELVRMLNDTSLDLLEL"
     gene            912..1571
                     /locus_tag="VGC27_02785"
     CDS             912..1571
                     /locus_tag="VGC27_02785"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0281532.1"
                     /translation="MAKTWSADNPKAALMTRKRAVIVDAALKAFLASGYAEASVNQIA
                     AAAGVSIKTLYRHFESKDDLFSAVMQAACGKMPETGSPGDDPEPPPAWYDAPPSEALP
                     QAGEEYLRHILSQDQLALYRVVTRDAHRFPELGRRYLQETAGGRDAKFAGYLDLWAER
                     EGWVIHDKRAAAQVFAGLLKARMFDDVLLGLRQPSDAEIAAQARHAALSMLLLMKDGR
                     F"
     gene            complement(1864..>2422)
                     /locus_tag="VGC27_02790"
     CDS             complement(1864..>2422)
                     /locus_tag="VGC27_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0281533.1"
                     /translation="PMRKYVGERIKKLREAQDMTLAQVCKLTGIDEEKLRAYEDGSVV
                     PSIGVVIQLSRVLGSKMEGLLHGGGTVSENLTICRSGDSLGGDQGDTEQSYAYASLTR
                     PGTAGHMMEPFLLSFDPKVAPGVPITHDGQEFVYIVEGVIELYYDGRNYRLERGDSVY
                     LDSSHPHTFHGLGESVAKMLAVVSS"
BASE COUNT          460 a          757 c          757 g          448 t
ORIGIN      
        1 gagttcgaga agatcgaggc tcgtgtcgtt cagcattctc acgagttcga tgcattccct
       61 gctggtgaaa ccgccacgct gaaaatcgga agagttgagc ttggcgccga tcggaaagcc
      121 tggtccgacc gcttcgcgga cggccgccag aaccgccatc aggaagcggg cccggttctc
      181 gagggagccg ccccatttgt ccttgcgccg gttgctcagc gggctgagaa attgcgagat
      241 cagatagccg tgcgcggcat gcagcgtgac cccggtaaag cccgcttcgc gcacttgccg
      301 ggcggcaaag ccgaattgac cgattgcgcg ctcgatctgg gattccgtca tcgcccgagg
      361 ttcggcaaaa ttgaagccgg cgccccggat cacgtcgagt tcgaccgcgg acggcgccag
      421 cggcgccgga ttgaatgcgg catcgacctg ccgtccggta tggctgagtt gcgcccagaa
      481 atgcgccccg cccgacttgc ccgcagccgc cagcttcgca agctgctcct tgccggcccc
      541 gtcgcgaatg accaggttga gcgggcgctc aagatgatcg ggatcgacct ggatgttgcc
      601 ggtaaacagc agcccggcgc ccgacgtcgc ccaccgccga tataaagttt caagccgtgg
      661 cgtagagtgg ttgtccacat cggccatgcc ttcgctcatc gccgttttgc agaggcggtt
      721 cgaaagcacg gcgccgcaag gcagcgtgag gggctgagag agagggtgca tgacgtctcc
      781 atagggcttg ccccggcaga cttgcgctcc cgaagctgtg gtcgtatttg taacacatgc
      841 gacagcgact gctgtctctt atacgacagt gataactgtt gtatggattg tcaagcggta
      901 tgggagccct gatggcgaag acctggtcgg cagacaaccc gaaggcggcg ctgatgacgc
      961 gcaagcgtgc cgtcatcgtc gatgccgcgc tgaaggcgtt tctggcgtcc ggctatgcgg
     1021 aggcttcggt caatcagatc gccgcagcgg caggggtgtc gatcaagacc ctctatcgtc
     1081 atttcgagag caaggacgat ctgttcagcg ccgtgatgca ggcggcgtgc ggcaaaatgc
     1141 cggaaacggg ttcccccgga gacgatccgg agccaccacc ggcttggtac gacgccccgc
     1201 cgtccgaggc gctcccacag gccggggagg agtacctccg ccatatcctg tcgcaagatc
     1261 aattggcgct ctaccgggtt gtcacccgcg acgcgcatcg ctttcccgaa ctcggccgcc
     1321 gctacttgca ggagacggcc ggcggccgtg acgccaagtt cgccggctat cttgatctat
     1381 gggcggaacg cgagggctgg gtcatccacg acaagcgcgc cgccgcccag gtgtttgccg
     1441 gcctcttgaa ggcccgcatg ttcgacgacg tgctgttggg gctgcgccaa ccgtcggatg
     1501 ccgagatcgc cgctcaggcg cgccatgccg cgctgagcat gctcttgctc atgaaggacg
     1561 gacgtttcta gagcgcaatc cggcaaaatg gtcggtagca gcagcgtgcc gacgcggttt
     1621 gccgtcagat tgcgcgaaaa tcaaggaatc tagagcttga tccgattcaa ttaaatcgga
     1681 tcaagctcta gcggccgccg accagccaaa tccggcgatg aacagatagc agcagccggc
     1741 aggaatcaaa agcgccgtcg tgaggccatg caaatccgtg acccggccgg aaacgcatgg
     1801 gatcacggag ccgcccgcga taaaggccgc atcgctcatc gcatcttgct gccgcgccac
     1861 cgcttacgaa cttaccactg cgagcatttt cgcgacgctc tctcccagcc catgaaaggt
     1921 atggggatgc gaggaatcga gatagacgct gtcgcctcgt tcgagcctgt agttgcggcc
     1981 gtcgtaatag agttcgatga cgccttcgac gatgtagacg aattcctgcc cgtcatgcgt
     2041 gatcggcacg cccggagcca ccttgggatc gaaggagagc aggaacggct ccatcatgtg
     2101 tcccgcggtg cccggccggg tcagcgacgc gtaggcatag ctctgctcgg tgtcgccctg
     2161 gtcgcccccc agcgaatccc ccgaacggca tatcgtgagg ttttcggaga ctgtgccgcc
     2221 gccgtgcagc aagccttcca tcttcgagcc gagcacgcgc gaaagctgga tcaccacgcc
     2281 gatggaggga acgacgctgc cgtcctcgta ggcgcgcaac ttttcctcat cgatgccggt
     2341 cagcttgcac acctgcgcca gcgtcatgtc ctgcgcctcg cgcagcttct tgatccgttc
     2401 gccgacatat tttctcatgg gc
//
LOCUS       DASWPU010000123         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_62242,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000123 DASWPU010000000
VERSION     DASWPU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62242"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1101)
                     /locus_tag="VGC27_02795"
     CDS             complement(169..1101)
                     /locus_tag="VGC27_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HEY0281534.1"
                     /translation="MSDSAEPIFAWVALLGVGLIGSSMAHAMRRAGVAGHIAGYSYRP
                     ETLERARIVGFADTLHADAASCVKDADLVVLATPVGSYAALAEQIAPHLKRGAILSDV
                     GSVKSAVIRDVGPYVPEGVHFIPAHPIAGTEQSGPEAGFAELFDGRWCILTPPPGTDP
                     AALERLKRFWRKLGSEVEVMEARHHDLVLAITSHLPHLIAYNIVGTADDLATVTQSEV
                     IKYSAGGFRDFTRIAASDPTMWRDVFLNNREAVLEVLGRFSEDLSALQRAIRWGDGET
                     LFNLFTRTRAIRRSIVDAGQDTPAADFGRGKKKP"
     gene            complement(1098..2189)
                     /gene="hisC"
                     /locus_tag="VGC27_02800"
     CDS             complement(1098..2189)
                     /gene="hisC"
                     /locus_tag="VGC27_02800"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY0281535.1"
                     /translation="MTPTPKPGVLEIAPYVGGRGSVAGVADPIKLSSNESALGPSPLA
                     LAAYAEAAKGVAVYPEGSARILREAIGATYGLDASRIVCGNGSDELLTMLANAYLRPG
                     DEVLYSEHAFLVYRIATLANSAVPVAVPENNLHADVDAMLARVTAKTRLVYLANPNNP
                     TGTYLPRDEVRRLHAGLPPEALLVIDAAYADYVRRNDYEPGIELVADFPNVVMTRTFS
                     KVFALAGLRIGWAYCPAAVADALGRIRAPFNVSVPAQCAGVAALKDRAHVARSVEHNE
                     RWRAWLTDSIRATGLRVDDSVANFVLIHFPHAEGKTAADADRFLCARGLILRQTGNYG
                     LPDCLRLTVGSEEANRKVAASLADFMGRT"
     gene            2366..3529
                     /locus_tag="VGC27_02805"
     CDS             2366..3529
                     /locus_tag="VGC27_02805"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY0281536.1"
                     /translation="MGPKFLRPLMAPDAEKGQAAHFGPDQALALDCGRTLAPWTLAYM
                     TYGELNEERSNAVLVCHALTGDQFVASAHPVTGKPGWWETMVGPGKPIDTSRFFVICA
                     NVVGGCMGSSGPADIDPETGKPFGLSFPLVTIRDMVRAQAMLLDALGIEKLLCVTGGS
                     IGGMQALQWAASYPERVVSAVLIACAAKHSAQNIAFHEVGRQAIMADPDWHGGTYQLH
                     GTQPSKGLAVARMAAHITYLSEAALQRKFGRNLQNPGGISFAFAPDFQVESYLHHQGM
                     TFVDRFDANSYLYITRATDYFDLAADYGGALAEAFRGSKTRFCILSFTSDWLYPTVEN
                     KRIAHALNAAAATVSFVEIETDRGHDAFLLDEPEMFAAVRGFLNSVAASQGVA"
     gene            3529..4137
                     /gene="metW"
                     /locus_tag="VGC27_02810"
     CDS             3529..4137
                     /gene="metW"
                     /locus_tag="VGC27_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEY0281537.1"
                     /translation="MANMRPDLAAIAEMIPPGARVLDVGCGDGTLLEYLTTAKQVDGR
                     GLELSQQNVNACVARGLAVIQGDADTDLVEYPSHVFDFVILSQTIQATRNPKSVLGHL
                     LRIGRRTAVSLPNFGHWRVRLSLLARGRMPRTKALGYEWYDTPNIHLCTIADFAALAE
                     EAGADIEHALSLNESGRTRPMQTDAWSPNLFAEGAIFLLRGR"
     gene            complement(4278..>4644)
                     /locus_tag="VGC27_02815"
     CDS             complement(4278..>4644)
                     /locus_tag="VGC27_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281538.1"
                     /translation="LRQLWRVLAPEGRLLLVAPNRASLWAQMEASPFACGRPFHKGEL
                     DALLRDTLFEPLRWDRALYLPPFSGRRLARMGAGWERLGRRFLPGLAGVHLVEAGKSL
                     YGLAKPHKLKRAEAALARA"
BASE COUNT          823 a         1543 c         1499 g          779 t
ORIGIN      
        1 tcgtagcagc agtgtgcgaa cgggctattc agttgaaacc tgctaagacc atcaaagaag
       61 agcgcagcga gaattccttg ggccatgcgc cagcgtcacc tgggtggccc gcattcgcgg
      121 gccatgacac cgtggttagg tgcgtcacct tgctgctgaa tcgctttatt agggtttttt
      181 cttgccgcgg ccgaagtccg ccgccggcgt atcctggccg gcatcgacga tggagcgccg
      241 gatcgcgcgc gtgcgggtga acagattgaa cagcgtttcg ccgtcgcccc agcggatggc
      301 gcgctgcaag gcgctcagat cctcgctgaa gcggcccagg acttcgagca ccgcctcgcg
      361 gttgttgagg aaaacgtcgc gccacatggt cggatcggag gccgcgatgc gggtgaagtc
      421 gcgaaagccg ccggcggaat atttgatcac ttcgctctgg gtgacggtgg cgagatcgtc
      481 ggcggtgccg acgatgttat aggcgatcag atgcggcaga tgcgaggtga tcgccagcac
      541 cagatcgtgg tggcgcgcct ccatcacctc cacctcgctg ccgagcttgc gccagaagcg
      601 cttcagcctt tccagcgcgg ccgggtccgt tccgggcggc ggggtcaaaa tgcaccagcg
      661 cccgtcgaac aattcggcga agcccgcttc ggggcccgat tgttcggtgc cggcgatcgg
      721 atgggcggga atgaaatgca ccccttcggg cacatagggg ccgacgtcgc ggatcaccgc
      781 gctcttgacc gagccgacat cgctgaggat cgcgccgcgc ttgagatgcg gcgcgatctg
      841 ttcggccagc gccgcatagc ttcccaccgg cgtcgccagc accaccagat cggcgtcctt
      901 gacgcaggac gcggcgtcgg cgtgcagcgt atccgcgaac cctacgatgc gcgcgcgctc
      961 cagcgtttcg gggcgatagc tgtagccagc gatgtggccg gcaacgcccg cacgccgcat
     1021 ggcatgggcc atcgacgaac cgatcaggcc gacgccgagc agcgcgaccc aggcaaaaat
     1081 cggctccgct gaatcgctca tgttcgtccc atgaaatcgg caaggcttgc ggcgaccttg
     1141 cggttggcct cttcgctccc cacggtgagc ctgaggcaat cgggcagacc gtaattgccg
     1201 gtttggcgca ggatcagtcc gcgcgcgcac agaaagcggt cggcgtccgc cgccgtcttt
     1261 ccctcggcat gcggaaaatg aatgagaacg aaattggcga cgctgtcgtc gactctcagg
     1321 cccgtggcgc ggatggaatc ggtcagccag gcgcgccagc gctcattgtg ctcgaccgaa
     1381 cgggcgacat gggcgcgatc cttgagcgcc gcgacgccgg cgcattgggc cggaaccgaa
     1441 acattgaacg gggcgcggat gcggcccagc gcatcggcga ccgctgccgg gcaataggcc
     1501 cagccgatgc gcagccccgc cagggcaaaa accttggaga aggtgcgcgt catcaccacg
     1561 ttggggaaat ccgccaccag ctcgatgccc ggctcgtaat cgttgcggcg gacgtaatcg
     1621 gcataagccg cgtcgatcac cagcagcgcc tcgggcggca ggccggcgtg caggcggcgc
     1681 acctcgtcgc gcggcagata ggtcccggtc ggattgttgg gattggcgag atagaccagg
     1741 cgggtcttcg ccgtcacccg cgccagcatg gcgtccacat cggcatgcag attgttctcg
     1801 ggcaccgcaa ccggcacggc actgttggcc agcgtcgcga tgcgatagac gaggaaagca
     1861 tgctcgctgt acagcacttc gtcgccgggg cgcagataag cgttggcgag catggtgagc
     1921 agctcgtcgg agccgttgcc gcagacgatg cgcgaagcat ccaggccgta ggtggcgccg
     1981 atggcctcgc gcaggatgcg ggcgctgcct tccgggtaaa ccgccacgcc cttggccgcc
     2041 tctgcatagg cggcgagcgc cagcggactc ggccccagcg ccgattcgtt ggaggacagc
     2101 ttgatcggat cggccacgcc ggccacgctg ccgcggccgc cgacataggg cgcaatctca
     2161 agcacgcctg gtttgggcgt tggcgtcatc cgctgttctc cagtcacccc gtcaaaaagg
     2221 ccggctttcc ggggcagcct tgatagagcc gggccggtgc aaaatcaaag cgacacagaa
     2281 tcaagcgttt cgttgacatg ggggatggct ggccttagca aacccgcctt cccggcccgc
     2341 atgggcgggg caaagggtct caccgatggg tccaaaattc ctccggccgc taatggcgcc
     2401 ggatgcggaa aaaggccagg cggcccattt cgggcccgac caggcgctcg cgctcgactg
     2461 cgggcgcacg ctggcgcctt ggaccctcgc ctatatgacc tatggcgagc tgaacgaaga
     2521 gcgctccaac gcggtgctgg tctgccatgc gttgaccggc gaccagttcg tggccagcgc
     2581 ccatcccgtc accggcaagc cgggctggtg ggaaaccatg gtggggccgg gcaagcccat
     2641 cgacacaagc cgctttttcg tcatctgcgc caacgtcgtc ggcggctgca tgggctccag
     2701 cggaccggcc gatatcgatc ccgagaccgg caagcctttc gggctctcct ttccgctggt
     2761 caccatccgc gacatggtgc gcgcccaggc catgctgctc gacgcgctcg gcatcgaaaa
     2821 gctgctttgc gtcaccggcg gatcgatcgg cggcatgcaa gcgctgcaat gggccgcgag
     2881 ctatcctgag cgcgtcgtct cggccgtgct gatcgcctgc gcggcgaaac actcggcgca
     2941 gaacatcgcc ttccacgaag tcggccgcca agcgatcatg gccgaccccg actggcacgg
     3001 cgggacttat cagcttcacg gcacgcagcc atccaaaggg ctggccgtcg cgcgcatggc
     3061 cgcgcacatc acctatcttt cggaagcggc cttgcagcgc aaattcggcc gcaacctgca
     3121 aaatccgggc ggcatctcct tcgccttcgc gcccgacttc caggtggaat cctatctgca
     3181 ccatcagggc atgaccttcg tcgaccgctt cgatgccaat tcctatctct acatcacgcg
     3241 ggcaacggac tatttcgatc tcgccgccga ttacggcggc gccttggcgg aagcttttcg
     3301 cggttcgaag acacgcttct gcatcctctc cttcaccagc gactggctgt atcccacggt
     3361 cgagaacaag cgcatcgcgc acgcgctcaa cgccgccgcc gccaccgtca gcttcgtgga
     3421 gatcgagacc gaccgcggcc atgacgcctt cctactcgac gagccggaaa tgttcgcggc
     3481 cgtgcgcggc ttcctgaact cggtcgcggc cagtcaagga gtggcgtgat ggcgaacatg
     3541 cgtcccgacc tggcggccat cgccgaaatg atcccgccgg gcgcgcgcgt gctcgacgtc
     3601 ggctgcggcg acggcacgct gctcgaatat ctgacgacgg ccaagcaggt ggacgggcgc
     3661 ggcctcgaac tgtcgcagca gaacgtcaac gcctgcgtgg cacgcgggct ggccgtcatc
     3721 cagggcgatg ccgataccga tctggtggaa tatccgtcgc acgttttcga tttcgtgatc
     3781 ctgagccaga ccatccaggc cacgcgcaat cccaaaagcg tgctcggcca cctcttgcgc
     3841 atcggcaggc gcacggccgt gtcgctgccc aatttcggcc attggcgggt gcgcctcagc
     3901 ctcttggcgc gcggacgcat gccgcgcacc aaggcgctgg gctacgaatg gtacgacacg
     3961 cccaacatcc acctctgcac catcgccgac ttcgcggccc tggccgagga agcgggcgcc
     4021 gatatcgagc atgcgctgtc gctgaacgaa agcggccgca cccgtccgat gcagaccgat
     4081 gcctggagcc cgaacctctt tgccgagggt gcgatcttcc tgttgcgggg gcgctagaaa
     4141 aatcgttcgt caatgcgcta gagcttaatc cgatttaatt gaatcggatc aaactctaga
     4201 ttccttgatt ttcgcgcaat ctgacggcaa accgcgtcgg cacgctgctg ctaccgacca
     4261 ctttgccgga ttgcgctcta agcgcgcgcc aaggcggctt cggcgcgttt gagcttgtgc
     4321 ggcttggcca agccgtaaag ggatttgccg gcttcgacca gatgcacccc ggcgaggccg
     4381 ggcagaaacc tccggcccag cctttcccag cccgccccca tccgcgccag cctgcggccg
     4441 ctgaagggcg gcaggtagag cgcgcggtcc cagcgcagcg gttcgaacag cgtatcgcgc
     4501 aacaatgcgt ccagctcgcc cttgtggaac ggccggccgc aagcgaaggg cgaggcttcc
     4561 atctgcgccc acaggctggc gcggttgggg gcgacgagca ggagccgccc ctccggcgcc
     4621 aggacgcgcc aaagctgacg cagc
//
LOCUS       DASWPU010000124         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_74303,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000124 DASWPU010000000
VERSION     DASWPU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74303"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..936
                     /gene="kduI"
                     /locus_tag="VGC27_02820"
     CDS             88..936
                     /gene="kduI"
                     /locus_tag="VGC27_02820"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEY0281539.1"
                     /translation="MYKKIYQATHPDSMASADNQTLRERYLIGGLFEAEEVSLTYSHS
                     ERLVIGGAMPVKKAVGLPVQEAPASAKGKPFLERRELGVVNVGAGSGSVTVDGKTYEM
                     IPEDGLYVPMGSAEVAFASLDPAKPAKFYLASTPAHARFETVKLSVEKSVPLERGALA
                     TSNERTIRQYIVPAICKSCQLLLGVTTLKTGSVWNTMPPHLHDRRSEVYFYFGLKAED
                     RVFHFMGEPDNMRHILVANEEAVISPPWSIHMGAGTSNYTFIWAMGGENLDYTDMDVL
                     DICQLR"
     gene            945..1703
                     /gene="kduD"
                     /locus_tag="VGC27_02825"
     CDS             945..1703
                     /gene="kduD"
                     /locus_tag="VGC27_02825"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HEY0281540.1"
                     /translation="MNPNPFSLEGKVALVTGANTGLGQGIAVALAQAGADIAVAGFVA
                     PLETAALVKAAGRRFLSIDANLMSIEPVGRVVDETVQRLDRLDILVNNAGIIRRADSL
                     EFSEKDWDEVMNVNIKALFFMCQAAGRQMVKQGSGKIINIASMLSFQGGIRVPSYTAS
                     KSGVAGITRLLANEWAAKNINVNAIAPGYMATDNTQQLRQDEARSKEILARIPAGRWG
                     EPADVGGTAVFLASAAANYLNGAIIPVDGGWLAR"
     gene            1748..3343
                     /locus_tag="VGC27_02830"
     CDS             1748..3343
                     /locus_tag="VGC27_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEY0281541.1"
                     /translation="MAPVSQMAMARRDVLLASLATAAASSLPTSVAIARGAAPVARTT
                     AGLVRGTVESGIATFKGIRYGADTRPRRFMPPLRPKPWRGVRDALAYGPACPQSRIKE
                     KVSEDCLFLNVWTPALHDGGKRAVMVYIHGGAYSSGSGSANITDGVNLCKRGNVVVVT
                     LNHRLNAFGYLYLAMLVGAEFADSGNAGQLDLILALEWVRDNIAEFGGDPDKVMVFGQ
                     SGGGAKIATMMAMPAAKGLFHRAATMSGQQLTASGPLHATRRAEVALSALGVAKDNLD
                     RLKEVPAEELVKALKAADPVIGSGGVYFGPVLDERNLTRHPFYPDAPAQSAGIPMMIG
                     NTHDETRAFITDDWAYRLTWEELPSRLAENMRVDILPSLVIEKYKAWFPGISPSDLFF
                     LATTASRSWRAAIVEDELRAQAGTPAFAYQMDWPSPLGDGRRRAQHMIDIPLAFDNTG
                     KDGAITGNGENARAMAAIVSETFIAFAKTGDPNNKLLPVWKPYTLPGRETMVFDVPPH
                     LENDPRGEQRRLFEKVPFIQQGT"
BASE COUNT          626 a         1071 c         1137 g          605 t
ORIGIN      
        1 gttgcacggg tgttggtcct gggcgtatga caccggttac caaatcaagg ggcgcatggg
       61 acgcgcgaac agcatagagg taaggcgatg tacaagaaga tctaccaagc gacgcacccc
      121 gacagtatgg cgtcggcgga caaccagacc ttgcgggagc gctatctgat cggcgggctg
      181 tttgaggcgg aagaggtgtc gctgacctat tcgcacagcg agcggctggt gatcggaggg
      241 gccatgccgg tgaagaaggc ggttgggctg ccggtgcaag aggcgcccgc ctcggccaag
      301 ggcaagccgt tcctggagcg gcgcgagctt ggggtggtca atgtcggcgc cggctccggc
      361 agcgtgacgg tcgacggcaa gacctacgag atgatccctg aggacgggct ttacgtgccg
      421 atgggcagcg ccgaggtggc gttcgcgtcg ctggacccgg ccaagccggc caagttctat
      481 ctggcctcga cgccggcgca tgcgcgtttt gagacggtga agctctcggt cgagaagtcg
      541 gtgccgctgg agcgcggcgc gctggcgacc tccaacgagc gcaccatccg ccagtacatc
      601 gttccggcga tctgcaaatc ctgccagctt ctgttggggg tgaccacgct gaagaccggc
      661 agcgtgtgga acacgatgcc gccgcacctg cacgaccgcc gctcggaggt ctatttctac
      721 ttcggcctga aggcggaaga ccgggtgttc cacttcatgg gcgagcccga caacatgcgt
      781 cacattctgg ttgccaacga ggaagcggtg atctcgccgc cctggtcgat tcacatggga
      841 gcggggacca gcaattacac cttcatctgg gcgatgggcg gggagaacct cgactatacc
      901 gacatggatg tgctcgatat ctgccagctt agataggggt tgtgatgaat ccgaatccgt
      961 tcagtctcga aggcaaggtg gcattggtga ccggggccaa caccggtctt ggccagggca
     1021 tcgcggtggc gctggcccag gcgggggccg acatcgcggt ggcgggcttt gttgcgccgc
     1081 tggagaccgc ggcgctggtg aaggcggcgg ggcggcggtt tttgtcgatc gacgccaatc
     1141 tgatgagcat cgagccggtg ggacgggtgg tggacgagac ggtgcaacgg ctggaccggc
     1201 tggacatcct ggtcaacaac gcgggcatca tccggcgcgc cgattcgctg gagttctcgg
     1261 agaaggactg ggacgaggtg atgaacgtca acatcaaggc gctgttcttc atgtgccagg
     1321 cggcggggcg ccagatggtg aagcaaggct ccggcaagat catcaacatc gcctcgatgc
     1381 tgtcgttcca gggcggcatc cgggttccct cctacaccgc gagcaagagc ggggttgcgg
     1441 gcatcacccg gctgttggcc aacgaatggg cggcgaagaa catcaacgtc aacgccatcg
     1501 cgccgggcta catggcgacg gacaacaccc agcagttgcg ccaggacgag gcgcggtcca
     1561 aggagatcct ggcgcgcatc cccgccgggc gctggggcga acccgccgac gtcggcggca
     1621 ccgccgtctt cctcgcctcc gccgccgcaa attacctcaa cggcgccatc atccccgtcg
     1681 acggcggttg gctcgctcga taggtgagat gaacgggtcg acacgaagcg agacggagga
     1741 cggcctaatg gcaccagtct cccaaatggc gatggctcgc cgcgacgtgc tgctggcctc
     1801 gctcgccacc gccgccgcaa gcagcttgcc aacaagcgtc gccatcgccc gcggcgccgc
     1861 gcctgtcgcg cgcacgacgg ccggcctggt acgcggtacc gtcgagtccg gcatcgccac
     1921 cttcaagggt atccgctatg gcgccgacac gaggcctcgc cgcttcatgc cgccgctgcg
     1981 gccgaagccc tggcgcggcg ttcgcgatgc gctcgcctac ggtccggcct gtccgcaatc
     2041 gcgcataaaa gagaaggtga gcgaggactg cctgtttctc aacgtctgga cgcccgcttt
     2101 gcacgacggc ggcaagcgcg ccgtgatggt ctatatccac ggcggggcct attccagcgg
     2161 ttccggctcg gcgaacatca ccgacggcgt gaatctgtgc aaacgtggca acgttgtggt
     2221 cgtcacgctc aatcatcgcc tcaatgcctt cggttatctc tatctggcga tgctcgtcgg
     2281 agcggaattc gccgattccg gcaatgccgg gcagctcgat ctgatccttg cgctcgaatg
     2341 ggtacgcgac aatatcgcgg agttcggcgg cgatccggac aaggtgatgg tgttcggcca
     2401 gtccggcggc ggcgccaaga tcgcgaccat gatggcgatg ccggcggcga agggcctgtt
     2461 tcatcgcgcc gcgacgatga gcggccagca attgaccgct tcgggtccgc tgcacgccac
     2521 ccggcgcgcc gaggttgctc tttccgctct tggcgtcgcc aaagacaatc tcgaccgcct
     2581 caaagaggtt ccggccgaag agctggtgaa ggcgctcaag gccgccgatc cggtcatcgg
     2641 ttcgggcggc gtctatttcg gaccggtgct cgacgaacga aatctgacgc gccatccgtt
     2701 ctatcccgac gcgcccgcgc aatcggccgg cattccgatg atgatcggca atacgcatga
     2761 cgagaccagg gccttcatca cggatgactg ggcctatcgt ctcacttggg aggaattgcc
     2821 ctcgcgtctt gccgagaata tgcgcgtgga catcctgccg tcgctggtca ttgagaaata
     2881 caaagcgtgg tttcccggca tttcaccgtc cgacttgttc tttctggcga cgacggcatc
     2941 gcgatcctgg cgcgccgcaa ttgtggaaga cgagttgcgc gcccaggccg gcacgcccgc
     3001 cttcgcctat cagatggatt ggccttcgcc gctcggcgac ggccgccgcc gcgcccagca
     3061 catgatcgat attccgctcg ccttcgacaa caccggcaag gacggcgcga tcaccggcaa
     3121 tggcgaaaac gcccgggcga tggccgccat cgtgagcgaa acctttatcg ccttcgccaa
     3181 gacgggcgat cccaacaaca agctgctgcc cgtctggaag ccctacacgt tgccgggccg
     3241 ggaaacgatg gtgttcgacg tgccgccgca tctggaaaac gacccgcgcg gagaacagcg
     3301 ccgcttgttc gaaaaggtgc cgtttatcca gcaagggacg taagcgcgcg cacgctcctg
     3361 cgaatttgcc tgcctgcttt gtttacgccg ggagagtaaa cggcgtcggt gattcccggc
     3421 gctcaatccc ctttaatca
//
LOCUS       DASWPU010000125         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_98844,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000125 DASWPU010000000
VERSION     DASWPU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98844"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VGC27_02835"
     CDS             <1..223
                     /locus_tag="VGC27_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281542.1"
                     /translation="TTPFQLDITKAAKPGANVLKVKVVNLWVNRLIGDKQPGVTKKYA
                     FTIIPTYKPDTPLRESGLMGPVLVQQVTP"
     gene            794..>2918
                     /locus_tag="VGC27_02840"
     CDS             794..>2918
                     /locus_tag="VGC27_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0281543.1"
                     /translation="MTRLDKHRARLFCGTAMIVAGFALCEPVTAYAQDSSEAVETVVI
                     TGSRIGVTSAYDAPTPVTVISAEEVKFSGTVNMEALLSMSPQFLPSTNGGQTGNTVQA
                     NGDSGAAWVNLRGLGEVRNLVLVNGRRFAIQGTRMTTDINTIPASLVEQTEVVTGGNS
                     AVYGSDAISGVVNFIMKKNFEGVEGNAQYTFDQYTTSPIYNFDLTVGGNFDHDKGNVV
                     MAVNYYSRKGFTQPDHGHWTNAQYTDACVTTASYSPSAPGTRLAGANNGADCVAKGGV
                     NGFVVGGSGDTPNGFIRVVGGSPLVDYAGAPAELKTLYDAAGLTGMTSDGIFFTNDST
                     AYPAEYRRRTPDDLYNLIENNYMQIPQQRWTLNTFAHYDFNKYIQGYTEFHFSSNKVS
                     AQLTPSSLKDVEIINTHNPAFSPALQAVMDYYDQHETGTTTVTTGTYTSTTVPNDGLV
                     AMSVGKRYIENGKRRQDASRVAWRFAGGFKGELGSVSEDFLKDIAYDFYYTFARTQET
                     DSQGGSIAKSKVAQSIVTAVNGATPVCNFFGLEVMSADCVAAITVDSTVGTVAEMQDA
                     NLSFTGTAFDLPAGPVQFAAGAEWRYTMAMYTPDKYTSSGDVSGLNAAQPTTGSMVSH
                     EVYGEVRIPVLKDLPFVQDFSVSGAFRYSDYNLSAAHNIWTWSGGADWKVWPDLTLRG
                     QYQRATRAPNVGELWGGLASNTSTVF"
BASE COUNT          641 a          864 c          839 g          574 t
ORIGIN      
        1 gacgacgccg ttccagcttg acatcaccaa ggccgcgaag ccgggcgcga atgtgttgaa
       61 agtcaaggtg gtcaatctgt gggtcaaccg gctgatcggc gacaagcaac cgggtgtgac
      121 gaagaaatac gctttcacga tcatcccgac ctacaaaccc gacacgccct tgcgcgagtc
      181 cgggttgatg gggccggtac ttgttcagca ggtgacaccg tagtctgacc gccgatgtgg
      241 aagtccatat cggcggtact gcggcatttc gaacttagtc cctattggcg cggtgcggcc
      301 cgccagtacc ggaccatggc agattgtctt tacgaccggt gttttcgctt ctatgcaggg
      361 ctggaatgta gccgccgacc ctttatgaca ttttcatctc aacctgtggc aaaaatgtgt
      421 gtgtttctag caaccgaacc gagcaggcag cggttgtcta acagctttga aggaccgacc
      481 gtctctctga agagcgccca aacggcgcag aaaaccgcga aatccttact caactaggcg
      541 aggttggcag agtcgattcg tcgcgacgca ttgctgtttg taatcaatcg catattcgat
      601 tttaattcac attaccacgc aactatgtat agatatcgct cttcaatgat cacaagtgca
      661 catttgtgat cgattcaggg gcagcgaaat acagggtttc tggaattccc ggggggcagg
      721 accactcgcg ccagagccga tttctcgacc gccccaatga ggaaccatgg agagggaggc
      781 ctacgtaatg tacatgacga gactcgataa acaccgtgct cgcctttttt gcggcacggc
      841 aatgattgtt gccggcttcg ccctgtgcga accggtgacc gcctatgccc aagatagctc
      901 agaagcagta gagacagtcg taatcaccgg ctcgcgcatc ggcgtgacga gtgcttatga
      961 tgcaccgaca ccggtgacag tcatcagcgc cgaagaggtc aagttttcgg gcacggtgaa
     1021 catggaggcc ctgctcagca tgtcaccgca atttctgccc tcgaccaacg gcggccagac
     1081 aggcaacacc gttcaagcga acggcgacag cggtgcggcg tgggttaacc tccgcggtct
     1141 cggcgaggtg cgcaacttgg ttctggtcaa cggacggcgt ttcgccatcc aaggcaccag
     1201 gatgaccacc gacatcaaca cgatcccggc ttcgttggtt gagcagacgg aagtcgtgac
     1261 gggcggtaac tcggcggtgt acggctcgga tgccatctcc ggcgtcgtca acttcatcat
     1321 gaagaagaac ttcgagggtg tggaaggcaa tgcgcagtac accttcgacc aatacacgac
     1381 atcgccgatc tacaacttcg atctgaccgt cggcggcaat ttcgaccatg acaagggcaa
     1441 cgtcgtcatg gccgtgaact actatagccg caaaggcttc actcagcccg accacggtca
     1501 ttggacgaac gcccaatata ccgatgcctg cgtaaccacg gcctcgtaca gcccgagcgc
     1561 gcctggcact cggttagccg gcgcgaacaa cggggcggac tgcgtagcga agggcggcgt
     1621 caacggcttc gtggtgggcg gcagcggcga tacgccgaac ggcttcatca gagtggtcgg
     1681 aggtagtccc ctcgtggact atgccggtgc gcccgccgaa ttgaagacgc tctacgatgc
     1741 cgccggtctt accggaatga cgtcggacgg tatcttcttc acgaatgaca gcactgcata
     1801 cccggcagag taccgccgcc gtactcccga cgatctctac aatctgatcg aaaacaacta
     1861 catgcagatt ccgcaacagc gttggaccct caacacgttc gcgcattacg acttcaataa
     1921 atacatccag ggctatacgg aattccactt ctcgagcaac aaggtctcag cacagctgac
     1981 gccgtccagc ttgaaggacg tagagatcat caacacgcac aacccggcgt tcagtccggc
     2041 gttgcaggct gtgatggact actatgacca gcatgaaaca ggcaccacca cagtcacgac
     2101 cggcacctac acttccacca ccgttccgaa cgacggcttg gtcgccatga gcgtgggcaa
     2161 gcgctacatc gaaaacggca agcgccgcca ggatgccagc cgcgtcgcgt ggcgcttcgc
     2221 gggcggattc aagggcgagc tcggctccgt gagcgaagac ttcctgaagg acatcgctta
     2281 cgatttctat tacaccttcg cccggacgca ggaaacggat tctcagggcg gcagcattgc
     2341 gaagagcaag gtggcgcaga gcattgtgac cgcggtcaac ggtgctacgc cagtctgcaa
     2401 tttcttcggc cttgaagtga tgtctgccga ttgtgttgcg gccatcacgg tggactccac
     2461 cgtgggaacg gtcgcagaga tgcaggacgc caacctgagc ttcaccggca cggcgttcga
     2521 tcttccggcc ggcccggtgc agttcgcagc cggcgcggaa tggcgctaca cgatggccat
     2581 gtatacgcct gacaaataca ccagttcggg cgacgtctcc ggcctcaacg ccgcccagcc
     2641 gaccaccggc tcgatggttt cgcatgaagt gtacggcgaa gttcgtatcc cggtcctcaa
     2701 ggatctgccc ttcgtccagg acttctccgt cagcggcgcc ttccggtatt ccgactacaa
     2761 cctgtcggcg gcgcataaca tctggacgtg gtctggcggc gccgactgga aagtgtggcc
     2821 cgacctgacc ttgcgtgggc agtaccagcg cgccacccgt gcgccgaacg tgggcgagtt
     2881 gtggggtggt cttgcgtcga acacgtctac cgtgtttg
//
LOCUS       DASWPU010000126         5762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_7077, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000126 DASWPU010000000
VERSION     DASWPU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7077"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..668)
                     /locus_tag="VGC27_02845"
     CDS             complement(81..668)
                     /locus_tag="VGC27_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0281544.1"
                     /translation="MLQALRNREGLTQAQLAQRLDVNQTAISRWERSVDQPGMALRRR
                     IRDMHRNFAPSRQDRALKLRVQQSWNPTALVARGAMFLEINNAGVAEANAAGKACAGQ
                     SIYGAFGQMTDETTAVWEKTGIFDGNIALAITVNRLELHDGQVNYIRTVDTPYFTSEG
                     EVWCLCEIKRIDAAQYNTVKSQMGAATFSLPFDSL"
     gene            895..1860
                     /locus_tag="VGC27_02850"
     CDS             895..1860
                     /locus_tag="VGC27_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY0281545.1"
                     /translation="MRIRTSSVLAIGLLFAIAASTSARPQAAPLPEPKAPIVNPFKSV
                     APLAECLAQSSTLVSGHRGGIEPGYPENAIETFAHSVAQHPLVLEIDVRTSRDGVMVL
                     MHDATIDRTSTGKGAISDLTVAEIQAAALKDKEGRVTSFQIPTFEAAIRWAKGRAVLL
                     ADVKEDASVEAIAAMIGTHEAQSNVAVIVYSLGQAQRLHRADPNITILYPVETDAELE
                     ALQNSGVPLDNVVAWTGIEHERPEQWNLMRQRGLAVAFGTLFFIDPELARTGNDARYL
                     GLSREGIAMLATDRHHQAFEAIQRERDIYAALHRCKATPEASHPF"
     gene            complement(1896..5273)
                     /locus_tag="VGC27_02855"
     CDS             complement(1896..5273)
                     /locus_tag="VGC27_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281546.1"
                     /translation="MTRLNAYRVWVFRAAAVVLAVLLAQPAEAADKFAASTAGGYARL
                     LFTLEPAAQVKAQVTGEVLTISFDRKTDLTPTALAQALPQYIASARADPDGKTFRFAL
                     SQPVRVHTSASSGKIAVDLAPASYPGTPPDLPPPPPAPPKTLDLSKLTAIKVRSGAYQ
                     NFTRVVFDWPRNVPYTVFPGIGRLTVRFQAEAKMDFTPIQRQAPPWVKAAGWRIDHGT
                     TFVELETDSDSGFHDFRDGSHIVVDVLAPKTDADAYRPPGDAKPSVTAMPKQAARKQT
                     DAVSKAQAEAIASAAAKLKTSANEPARPATAPPAKLAPAANAPGNPATPAPAAAAPPP
                     AETATPSSVAPDIQAADSKLIRNGAVLTFPGAGRRGAAVFVRGLTAWVVLQNAAPLDA
                     AKLKSQLGDFPAAVDAASSDNVSILRITLKRPEKIAATAEGSALKVVIAPQTNANAMA
                     IGFARNQDDPARSSLSTLLPGATKAVTLIDPVAGDVLTVIPGAAGRAMLNERSFVEFT
                     ALETASGIVLEAYVDDLSVAIDASRVTITRPGGLALTPPSMPAGSSPEALARVGDGPC
                     FLDFARWKNATGGSFLATERRLRAAAARLKPEAANHARLALARFYLANGFAAEALGLV
                     KLMQSADPALQSDKQLQTIRAAADYGMGRTRDAHNDIAGVQFDADRHAALWRGLIDAA
                     LENWESARANFERASTVLDRYQPGMQARVHIAHANAALATGRLEIADAELSRLSEPLE
                     RPLMLEANLARARLYAAEKRRRDADRLFAAVEQGGDERIAAKAVYYRVGAGLAAGSLS
                     KDKAVNALERLRFRWRGDLLELNTLRKLASLYFAQQQWRNGLRTLRVAAQYFPNEDLS
                     GKAQDDMRVVFVDLFLKGKADAMPPVEALAVFYDFIELTPIGPEGDEMIRRMADRLVA
                     VDLLGPAADLLDYQVTKRLDGVARAQVATRLAMVQLMDHKPQAALETLRSTRISTLPD
                     DINHQRLLMEARALAEQKQWDEALDLIGVDQSPDTVRLRADIYWQSGNWSAAAQKAEE
                     ALGTSWSDPAPLGDEQRQQVMRAAISYSLANDEPGLERLRERFAAKMKESRDANAFDV
                     VTQRIDMHGLAFRDAAAKVASIDTLRAFMKDLQRRAE"
     gene            complement(5324..>5762)
                     /locus_tag="VGC27_02860"
     CDS             complement(5324..>5762)
                     /locus_tag="VGC27_02860"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281547.1"
                     /translation="AAIAERADLLAAAESRVDGKIAALKQLQDNIAELLGQRDAEEQK
                     QLASLVKAYTAMKPKDAARIFNNLSDDVLIQVARAMKSDALAPILAAMTPDAAQKLTV
                     KLANHLKLPELAAPALASSAAATAPQQISAAAPQAGAGTAAGK"
BASE COUNT          939 a         1841 c         1911 g         1071 t
ORIGIN      
        1 gcatgaggga ttggaaaatg ccctcaccct gagcttgtcg aagggtgatt gggaacaatt
       61 caatcagatt gaatcatgct ttagaggctg tcgaaaggca gcgagaacgt cgccgccccc
      121 atctgagact tgacggtatt atattgcgcg gcgtcgatcc gtttgatttc gcacaggcac
      181 cacacttcgc cctcgctggt gaaatacggg gtgtcgaccg tccggatgta gttcacttgg
      241 ccgtcatgaa gttcgagcct gttgacggtg atggcgagcg caatattgcc gtcgaagata
      301 ccggtcttct cccacacggc ggtcgtttca tcggtcattt ggccgaaggc gccgtagatg
      361 ctttgtccgg cacaggcctt tcccgcggcg ttcgcttcgg ccacacccgc attgttgatc
      421 tcaaggaaca tggcgccgcg cgcgaccagc gcggtcggat tccaggattg ctggacccgc
      481 agtttcaagg cgcggtcttg gcgcgagggt gcaaagttcc ggtgcatgtc gcggatgcgc
      541 cgcctaagcg ccatgcccgg ttgatcgacc gagcgctccc agcgggaaat cgcggtttgg
      601 ttgacgtcga gccgctgcgc cagttgcgct tgggtcaagc cttcccggtt gcgcagtgct
      661 tgcaacatcc gcggccagta tgcgtccgac accatcttcc acctcaggtt ggcagcgcat
      721 ccgctgcaag tacccagggc gccccatgtt tcaacgtctt atgcgttcca aagccgcata
      781 ggcgctttta gccgtcgtta ccacggacgt tttgcagttt tgcgaagcag ttgtcacaca
      841 ggcccgatac tcagccgacc tgcgacggtt cgaccgctct ggaggctttt caccatgcgc
      901 attcgcactt cttccgtgct ggcgatcgga cttctcttcg cgatcgcggc atccacgtcc
      961 gcccgcccgc aggctgcgcc gcttcccgag ccgaaggctc cgatcgtcaa tccgttcaaa
     1021 tccgtcgcac ccttggcgga gtgcctagcg cagagctcca ccctggtcag cggccatcgc
     1081 ggcggcatcg aaccgggtta ccccgagaac gccatcgaga ctttcgccca ttcggtcgcg
     1141 cagcacccgc tcgttctcga aatcgacgtc cgcaccagcc gcgacggcgt catggtgctg
     1201 atgcacgacg ccacgatcga ccgcacctcc acgggaaagg gagcgatcag cgacctgacc
     1261 gttgcggaga ttcaggcggc ggcgctcaag gacaaggagg ggcgcgtaac ctcgttccag
     1321 attcccactt tcgaggcggc gatccgctgg gcgaaggggc gcgccgtact gctcgccgac
     1381 gtcaaggagg atgcctccgt cgaagccatc gccgccatga tcggtaccca cgaagcgcaa
     1441 tcgaacgtcg ccgtcatcgt ctattcgctc ggccaggcgc agcgtctcca tcgcgccgac
     1501 ccgaacatta cgatcctcta tccggtcgaa acggacgcgg agctggaagc tttgcagaat
     1561 agcggcgttc cgctcgacaa tgtggtggca tggaccggta tcgagcacga gcgccccgag
     1621 caatggaact tgatgcgcca acgtgggctg gccgtagcct tcggcacgct gtttttcatc
     1681 gatccggaac tggcgcggac cggcaacgat gcccgttatc tggggctttc gcgcgaaggc
     1741 attgcgatgc tggctacgga ccggcatcac caggcttttg aagcgatcca gcgcgagcgg
     1801 gatatctacg ccgcccttca ccgctgcaaa gccacgcccg aggcctcgca tcctttctag
     1861 ccgcgcccgg aaagctggcg gagcgccggc tgctcttact ccgcgcggcg ctgcagatcc
     1921 ttcataaagg cccgcagcgt gtcgatggag gccactttgg cagccgcgtc gcggaaggcc
     1981 aggccatgca tgtcgatgcg ctgggtgacg acatcgaagg cgttggcgtc gcggctttct
     2041 ttcatcttcg cggcaaagcg ctcgcgcagg cgctccaggc cgggttcatc gttggcgagc
     2101 gaataggaaa tcgcggcgcg catgacctgc tggcgctgtt cgtcgccgag cggggccgga
     2161 tcgctccagc tcgtccccag cgcttcctcg gctttctggg cggcggcgct ccaattgccg
     2221 ctttgccaat aaatatcggc tctcagccgc accgtgtcgg gcgactgatc cacgccgatc
     2281 aggtcgagcg cctcgtccca ttgcttttgt tcggccaagg cccgcgcttc catcagcaga
     2341 cgctggtggt tgatgtcgtc gggcagggtg gagatgcgcg tgctgcgcaa ggtttcgaga
     2401 gccgcttgcg gcttgtgatc catcagctgg accatggcca gcctggtggc gacctgggcg
     2461 cgcgccacgc cgtcgagccg cttggtgacc tgataatcga gaaggtcggc ggcggggccc
     2521 aacagatcga cggccaccag acggtcggcc atgcgccgga tcatttcgtc gccctccggg
     2581 ccgatcggcg tcagctcgat gaagtcgtag aacacggcca gtgcttccac cggcggcatg
     2641 gcgtcggcct tgcccttcag gaacagatcg acaaaaacca cccgcatgtc gtcctgcgcc
     2701 ttgccggaca agtcttcgtt ggggaaatat tgcgcggcga cgcgcaacgt ccgcaacccg
     2761 ttgcgccatt gctgctgggc gaaatagagc gaggcgagct tgcgcagcgt gttcagttcc
     2821 agaaggtcgc cgcgccagcg gaagcgcagc cgctcgagcg cgttgacggc cttgtccttg
     2881 ctcaagcttc ccgccgccag cccggcgccg acgcgataat agacggcctt ggccgcaatc
     2941 cgctcgtcgc cgccttgttc gaccgcggcg aacagccggt cggcgtcgcg ccgccgtttc
     3001 tcggcggcgt aaaggcgcgc gcgggcgaga ttggcttcga gcatcagcgg tctttcaagc
     3061 ggttcggaga ggcgcgaaag ctcggcatcg gcgatctcca gccggccggt tgcgagcgcc
     3121 gcattggcgt gggcgatgtg gacgcgggcc tgcatgccgg gctggtagcg gtccagaacc
     3181 gtgcttgcgc gttcgaaatt cgcccgcgcg ctctcccaat tctccagcgc cgcatcgatc
     3241 agcccgcgcc acaaggcggc gtggcggtcg gcgtcgaact gcacgccggc gatgtcgtta
     3301 tgggcgtcgc gggtgcggcc catcccgtaa tccgccgccg cccggatggt ttgtagctgc
     3361 ttgtcgcttt gcagcgccgg atcggccgac tgcatcagct tgacgaggcc gagcgcttcg
     3421 gcggcgaatc cgtttgccag atagaaccgt gccagcgcca ggcgcgcatg attggcggcc
     3481 tcgggcttga gccgcgccgc ggccgcgcgc aagcgccgct cggttgccag gaagctgccg
     3541 ccggtggcgt tcttccagcg ggcgaaatcc aggaagcacg ggccgtcgcc gacgcgcgcc
     3601 agcgcttcgg gcgaggagcc cgccggcatg gatggcggtg tcagcgccag tccgcccggt
     3661 cgcgtaatcg tcacccgcga cgcgtcgatg gcaacggaca gatcgtcgac ataagcttcc
     3721 agcacgatgc ccgaagcggt ctcaagcgcc gtgaattcga caaagctgcg ctcgttcagc
     3781 atggcgcggc cggcggcgcc agggatcacg gtcagcacgt ctcccgcgac cggatcgatc
     3841 agcgtcaccg ccttggtggc tcccggcagc agcgtcgaaa gcgaggagcg ggcgggatcg
     3901 tcctggttgc gggcaaagcc gattgccatg gcgttcgcgt tcgtctgcgg ggcgatgacg
     3961 accttgagcg cggacccttc agccgtggcc gcaatctttt cgggccgttt cagggtgatg
     4021 cgcagaatgg agacgttgtc gctcgatgcc gcatcgaccg cggcgggaaa atcgccgagc
     4081 tgcgatttca gcttggccgc atcgagcgga gccgcattct gcaacaccac ccaagcggtc
     4141 aggccgcgta cgaacacggc cgcgccgcgc cggcccgcac ccgggaaagt cagaacggcg
     4201 ccattgcgga tgagcttgct gtcggcggcc tggatatcgg gcgctacgga cgatggcgtt
     4261 gcggtttcgg ccggcggcgg cgcggccgca gcaggtgcgg gcgtcgccgg gttgcccggc
     4321 gcgttggcgg cgggggccag ctttgccggc ggcgcggtcg ccggcctggc cggctcattc
     4381 gcggatgtct tcagcttcgc cgccgcagat gcgatggcct cggcctgcgc cttgcttacg
     4441 gcgtcggttt gtttgcgcgc cgcctgtttc ggcatggcgg tcacggacgg ctttgcgtcg
     4501 ccgggcggtc tgtaggcatc ggcgtcggtc ttcggcgcca gcacgtcgac gacgatatgc
     4561 gagccgtcgc ggaaatcgtg aaagccggaa tcgctgtcgg tctccagttc gacaaaagtt
     4621 gtgccatgat cgatgcgcca gcccgccgct ttcacccagg gcggggcttg gcgctggatg
     4681 ggggtgaagt ccatcttcgc ttccgcctgg aagcggaccg tcaatcttcc gatgccgggg
     4741 aacacggtgt agggaacatt gcgcggccag tcgaacacca cgcgggtgaa attctgatag
     4801 gcgccggaac gcaccttgat cgcggtcagt ttggacagat cgagggtttt cggtggcgct
     4861 ggcggcggtg gcggcaaatc cggcggcgtg ccgggatagc tcgcaggcgc cagatcgacg
     4921 gcgattttgc cggacgaggc gctggtatgg acgcgcaccg gctggctgag cgcgaagcgg
     4981 aaggttttgc cgtccgggtc ggcgcgtgcg ctggcgatat attgaggcag ggcttgcgcg
     5041 agtgccgtgg gcgtcaagtc ggtcttgcgg tcgaaggaaa tcgtcaggac ttcgccggtg
     5101 acctgcgctt taacctgggc ggccggttcc agcgtgaaca gcagccgggc atatcccccc
     5161 gccgtcgagg ccgcgaattt gtcggccgcc tcagccggtt gcgccagcag aacggcgagc
     5221 acaaccgccg cggcgcgaaa aacccaaacc cgatatgcgt tcaaccgcgt catgaaaatt
     5281 gccgtcgtcc agtccggtgt acccgatcaa ccttccgtag atcctatttg cctgccgccg
     5341 taccggcgcc cgcttgcggg gccgcggccg aaatctgttg aggtgccgtc gcggcggcgg
     5401 aagacgccag cgcgggcgcc gccaattcgg gaagcttcaa atggttcgcc agtttgacgg
     5461 tcagcttctg agcggcgtcc ggagtcatgg cggcgaggat cggcgccaag gcgtcggatt
     5521 tcatcgcccg cgccacctgg atcagcacat cgtcggaaag attgttgaag atgcgggccg
     5581 catctttcgg cttcatcgcg gtataggcct tcaccagcga agcaagctgt ttctgctcct
     5641 cggcgtcgcg ttggccgaga agctcggcga tattgtcctg aagctgcttg agggcggcaa
     5701 tcttgccgtc cacgcggctc tcggcggcag ccaaaagatc ggcgcgctcg gcgatggcgg
     5761 cc
//
LOCUS       DASWPU010000127         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_62305,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000127 DASWPU010000000
VERSION     DASWPU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62305"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGC27_02865"
     CDS             complement(<1..364)
                     /locus_tag="VGC27_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0281548.1"
                     /translation="MKTVLTSRAAIITGGPGVGKTTLVNSILLILRAKKVKCLLCAPT
                     GRAAKRLTETTGLEAKTIHRLLEVDPATGRFSRNEDNPVECDLLIVDETSMVDVLLMY
                     ALLRALPKAAALIMVGDVD"
     gene            complement(526..1301)
                     /locus_tag="VGC27_02870"
     CDS             complement(join(526..950,950..1301))
                     /locus_tag="VGC27_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_167331742.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0281549.1"
                     /translation="MYPSDLTDAQWVKLEPLLKPPRGNRHAGGRPRKYELRRVVDALL
                     YVVKTGCQWRQLPSNFPPWLSVHQQFRAWRDDGTWERVTKTLREQGRKAAGRSATPTV
                     AIIDSQSAKTVLKGGGRGYDAGKKIKGRKRHIAVDTQGNLLAVIVHSAGIQDRIAARA
                     VLMRLFCLFASIQTVFVDGGYTGTLIDWAKQMFGYTVQVIKRSDQHLFKVLPKRWIVE
                     RTFAWLNWSRRLSKDYELRHSSAESVIYIAFAHMLLRRST"
BASE COUNT          312 a          449 c          429 g          331 t
ORIGIN      
        1 gatccacgtc gccgaccatg atcagcgcgg cggccttggg aagagcacga agcagggcat
       61 acatgagcag tacatccacc atggatgtct cgtcgacgat gagcaggtcg cactccacgg
      121 gattgtcctc attgcgcgag aatcttcccg tggccggatc gacttccaac aggcggtgaa
      181 tggtttttgc ttccagcccg gtggtctctg tgagccgctt cgcggccctg ccggtgggag
      241 cgcagagcag gcacttcact ttcttggctc gcagaatcag gagaatcgag tttaccagtg
      301 tggtttttcc gacaccgggt ccgcccgtaa taatggcggc ccggctggtc agaacggttt
      361 tcaacgcttc ccgctggctc ggagcgagcg tcttcccggt tttcttctcg caccatgcga
      421 ctgccttctc aaaatcgatc ttcggataga ccggtccggc ggcagccagc cgcttgatct
      481 tcgtcgcaat cccgtcttcg gctttcctca gaatctgtct aaaaactaag ttgatcgtcg
      541 taggagcata tgagcgaagg cgatgtagat cacggattca gccgaggaat gtcgcaattc
      601 gtagtctttg ctgagacggc gtgaccagtt gagccaggca aaagtgcgtt cgacgatcca
      661 gcgtttgggt agaaccttga atagatgctg gtccgaacgt ttgatcacct ggaccgtgta
      721 gccgaacatt tgtttggccc agtcgatcag cgtgcccgtg tagccgccgt ccacaaacac
      781 ggtttggatg cttgcgaaaa ggcaaaacaa ccgcatcagc acggcacgag ctgcaatgcg
      841 gtcttggatg ccggccgaat gaacaatgac cgccagcaaa ttgccctgcg tgtctaccgc
      901 gatatgccgc ttgcgaccct tgatcttttt acccgcgtca tagccgcgcc gccccctttt
      961 agcaccgtct tggccgactg cgaatcgatg atcgccacgg taggcgtggc gcttcgtcca
     1021 gccgccttgc ggccctgttc gcgcagagtc tttgttaccc gctcccatgt accgtcgtcg
     1081 cgccacgccc ggaactgctg atgcacgctc agccagggag gaaagttgga aggcagctgc
     1141 cgccactggc agcccgtctt cacaacgtac aaaagggcgt ccaccacgcg gcgcagctca
     1201 tacttgcgag gacggcctcc agcatggcga ttcccacgag gcggcttcag caacggctct
     1261 aacttaaccc actgtgcgtc ggtcaaatcg ctcggataca tgcctctaag ctaagagcag
     1321 aaccgttccg tgcatattcc tccgcgggta cctttgacag tttttagaca ggttctaagg
     1381 ggaaaacgta ccgacaggca gacagagcag aacgaccacg cggcggaaga tacactccgc
     1441 tgcgtggaag tggacagagg cgcgtgcagc gcctccttga atggggcctt tgaatctact
     1501 ttccattagg tttttgcgcg a
//
LOCUS       DASWPU010000128         4731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_86465,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000128 DASWPU010000000
VERSION     DASWPU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86465"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VGC27_02875"
     CDS             <1..1341
                     /locus_tag="VGC27_02875"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY0281550.1"
                     /translation="DIGVNKGKIAKIGDLSKADTGERIDAKGLHILPGVIDTQVHFRE
                     PGNEHKEDLESGSRAAVLGGVTAVFEMPNTDPPTTTRAAIEDKLARAAKRMHCDYAFY
                     VGATQANVGALAALERMPGVCGVKAFLGSSTGTLLLDREDAILAALKGGARRMALHSE
                     DEARLKERKPLAGKGDVRTHPFWRDAETARASTERVLRLAREAGRRVHVLHVTTEDEL
                     PLLAAARNLATVETTPQHLTLSAPECYERLGTLVQMNPPIREARHREALWAAVGEGLI
                     DVIGSDHAPHARAEKDKTYPDTPSGMPGVQTLVTILLDHVNGGRLTLERFVDLTSAGA
                     ARIFGIAGKGRIARGYDADFTIVDLKAKKRIENSWVASKCGWTPFDGMETTGWALATI
                     IRGQTVMRERALTASGQGEPLRFVETLERFPKSGTRFSDNGTRFSNKKRDKTNS"
     gene            1669..2322
                     /locus_tag="VGC27_02880"
     CDS             1669..2322
                     /locus_tag="VGC27_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY0281551.1"
                     /translation="MNYVVLVVSGVLIGFFVALPIGPVNLICIRRTLQCGSFHGFVSG
                     LGAVLGDGLFASITGFGLTAVAQLIVGFSTALQLFGGLLLLSFGVRTFFTPPPRFDER
                     LAANENGASSHVRAMASTFVLTVTNPATLFGFTALFAGLSGLAGDNPSFLAASFVVLG
                     VFCGSTLWWLTLTTGVGLLHARISDHTVRVINEISGAVVAMFGVVILGHLVSQLLGW"
     gene            2477..3730
                     /locus_tag="VGC27_02885"
     CDS             2477..3730
                     /locus_tag="VGC27_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281552.1"
                     /translation="MTSEIAVMNQRAVALAADSAVTLIDAGVVVVRNDQRKLFNLANG
                     LPLGLMFFGVADMMGHPWEHLIEHFQKKVKPGPLPHVGDYAAKFVGMLDNLEEFFPRE
                     RQKDDYKRLLASVFRYVFHLAQYLREIGGAERAHVTDIAILQEAIEHVWKIYQFREDG
                     SARRDLACFPPGFAATVARDYAGAIDELIAYGFSSFNIDGNAVRRLRDIAVFCVVKDL
                     FLEDVTGLVFAGFGKEEHYPAVVTYFVSAIVGGIVKRTEASVDVIDGEVRSKIRIFAD
                     SEVTNAFIRGIDADLERRMYGTMQAMMQGLVEHVISAFPNVDQPRREGVKTQFRIQIL
                     PKYFDTFRAMMADYQQQTHINPVLRVLEIAARQELGETAHELVALNIFKKRIMAQHPT
                     VGGAIDVAVISRDSGFQWWKKQGGG"
     gene            3733..4161
                     /locus_tag="VGC27_02890"
     CDS             3733..4161
                     /locus_tag="VGC27_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281553.1"
                     /translation="MADTRRPDKAPRGALAEGAERFDRLARSVKDSQLRRTKTGPRPA
                     VTESFELSPEDAWAKKYMTHCIGPVCYTVEAMTEPPVFAKFGTAPEASVQGAGVQAEA
                     SEKAKNPPPLGRNAAELSPIGLVIGKPVGKRSWLGRLLGR"
     gene            4265..>4731
                     /locus_tag="VGC27_02895"
     CDS             4265..>4731
                     /locus_tag="VGC27_02895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0281554.1"
                     /translation="MRRFGFLVCALVFFAAFSSKAADNSLSAEANAAFLAAYDKKPGV
                     IIKPDGLRYRILHNGFGRRPRPQDYVTVNYTGSLINGQVFDGTEPGMPARFKANSLIS
                     GWTEALSLMRVGDRWEVVIPPSLGYGERGTPEGTIPPNQVLVFQLELIKTVPP"
BASE COUNT          889 a         1425 c         1505 g          912 t
ORIGIN      
        1 gacatcggcg tgaacaaagg caagattgcc aaaatcggcg atctctcgaa ggcggacacg
       61 ggcgagcgca tcgacgccaa aggcctccac atcctgccgg gcgtgatcga cacccaggtg
      121 catttccgcg agcccggcaa cgaacacaag gaagacctcg aaagcggcag ccgcgcggcg
      181 gtgctgggcg gcgtcaccgc cgtcttcgag atgcccaaca ccgatccgcc gaccacgacg
      241 cgcgccgcca tcgaggacaa gctcgcacgc gccgccaaac ggatgcattg cgactatgcc
      301 ttttatgtcg gcgccacgca ggccaatgtc ggcgcgctgg cggcgctgga gcgaatgccc
      361 ggcgtctgcg gagtaaaggc atttctcggc tcctccaccg gcacgctgtt gcttgaccgc
      421 gaggatgcga ttctcgcggc gctcaagggc ggcgcacggc gcatggcatt gcattccgag
      481 gacgaagccc ggctcaagga acgcaagcct ctagctggca aaggcgacgt acgcacgcat
      541 ccgttctggc gcgatgcgga aacggcgcgc gcttcgaccg agcgcgtgtt gcgtctggcg
      601 cgcgaggcgg gccgtcgcgt ccatgtgctg catgtgacga cggaagacga attgcctctg
      661 ctcgccgcgg cgcgtaatct ggccaccgtc gagaccacac cgcagcatct cacgctctcg
      721 gcccccgaat gctacgagcg gctcggaacc cttgtccaga tgaatccgcc gatccgcgag
      781 gcgcgccacc gcgaggcgct gtgggcggcc gttggcgagg gtctgatcga tgtcatcggc
      841 tccgaccatg ccccgcacgc acgcgccgag aaggacaaga cttatcccga cacgccgtcc
      901 ggcatgccgg gggtgcagac ccttgtgacc atcttgctcg atcatgtgaa tggcggccgg
      961 ctgacgctcg aacgtttcgt cgatctcacc agcgccggtg cggcgcgcat tttcggcatc
     1021 gccggcaagg ggcgcatcgc gcgcggctat gacgccgact tcaccatcgt ggacctgaaa
     1081 gcgaagaaga ggatcgagaa ttcctgggtc gcctccaaat gcggctggac gccgttcgac
     1141 ggcatggaaa ccaccggctg ggcgctcgcc acgatcatcc gtgggcagac cgtcatgcgc
     1201 gagcgtgcgc tgacggcatc ggggcagggc gaaccgctgc gcttcgtcga aacgctagag
     1261 cgcttcccga aaagtgggac ccggttttcg gataatggga cccggttttc gaataagaag
     1321 cgcgacaaga caaatagcta gagcaaatca ccgattccaa taaacggtga tttgctccag
     1381 gccctcgaac ggcgatcgtt cgacgggcca gggacacggc ttgatggtcc aaaaccaatt
     1441 caatttgtcg catgaccgcc cacttccccg atttacgaac ctttctatcc tgggggttct
     1501 gaaggagttt tgcccccaaa cggggttgcg tgtcgcgttt tgacttgcat gatggcgcgg
     1561 attcagttgc cgtaacgccc gcaatagctt caagcagcat cgagacaaag gaagtgcccg
     1621 catgaaggac cgcttcagga cttggtttgc agtcaccgcc ggggtcgaat gaactacgtt
     1681 gtcctcgtcg tctccggcgt gctgatcggc tttttcgtgg ccctgccgat cgggccggtt
     1741 aatctgatct gtatacgccg cacgctgcaa tgcggctcct tccacggctt cgtctccgga
     1801 ttgggggccg tactcggcga cggcctgttc gcgtccatca cgggtttcgg cctcacggcg
     1861 gtggcgcagt tgatcgtcgg cttctcgacc gcgttgcagc ttttcggcgg tttgctgctg
     1921 ctttccttcg gcgtacggac gttttttacg cccccgccgc ggttcgacga gcgattggcg
     1981 gcaaacgaga acggagcctc ttcgcatgtg cgcgccatgg cctccacctt tgtgctcacc
     2041 gtcaccaatc cggcgacgct gttcggcttc acggctttgt tcgcgggcct cagcgggctt
     2101 gccggcgaca atccgagttt cctcgcagcc tcctttgtgg tgctgggcgt gttctgcggt
     2161 tcgacgctgt ggtggctaac gttgaccacg ggtgtcggtc ttctgcacgc gcgcatcagc
     2221 gatcacaccg tgcgggtgat caacgagata tcgggcgccg tggtggcgat gttcggagta
     2281 gtcattctcg gtcacctcgt atcccagctt ctgggctggt aggcgcaaca taaaccacct
     2341 atgcgtgtgt actaattttt ggaatgtaca aattgtagca ggcggcggcg catcctaacc
     2401 aaattgcccg gcatcgctcc cctttcgggg ccggttgtga cattctacat gcaaagcgtg
     2461 gggcaaggct cggcggatga catccgaaat tgcggtgatg aatcagcgcg cggtcgcgct
     2521 tgcggcggat tccgccgtga ccctgatcga tgcgggtgtg gtggtcgtgc gcaacgacca
     2581 gcgcaagctc ttcaatctgg caaacgggct gcccctgggc ctgatgttct tcggcgtggc
     2641 cgacatgatg gggcatccct gggagcatct gattgagcat tttcagaaga aggtgaaacc
     2701 ggggccgctg ccgcatgtgg gcgactacgc cgcgaaattc gtcggcatgc tcgacaatct
     2761 cgaggagttc tttccgcgcg agcgccagaa ggacgattac aagcgtctgc tggcctcggt
     2821 gttccgctac gtgttccacc tggcgcagta tctgcgcgag atcggcgggg cggaacgcgc
     2881 ccatgttacc gacattgcga tcctgcaaga agccatcgag cacgtctgga agatctacca
     2941 gttccgtgaa gacggaagcg cgcgccggga tctcgcgtgc ttcccgcctg gcttcgccgc
     3001 gaccgtggcg cgcgattatg ccggcgcgat agacgaactg atcgcttacg gtttttcatc
     3061 cttcaacatc gacgggaacg cggtacggcg tttgcgcgac atcgcggttt tttgcgtggt
     3121 caaggacctg ttcctcgagg acgtgacggg attggtcttc gcggggttcg gcaaggaaga
     3181 gcattatccc gccgtcgtca cctatttcgt ctcggcgatc gtcggaggaa tcgtcaagcg
     3241 gaccgaagcc agcgtcgatg tgatcgacgg cgaggtgcgc tccaagatcc gcatcttcgc
     3301 cgacagcgaa gtcaccaacg ccttcatccg cggcatcgac gccgatctgg agcggcgcat
     3361 gtatggaacc atgcaggcga tgatgcaggg gctggtcgag catgtgatca gcgcctttcc
     3421 gaacgtcgat cagccgcggc gcgaaggcgt caagacgcag ttccggatac agattctgcc
     3481 gaagtatttc gacaccttcc gcgccatgat ggcggactac cagcagcaga cccacatcaa
     3541 ccccgtacta agggtgctgg aaatcgccgc ccgccaggaa ttgggggaga ccgcgcacga
     3601 acttgtcgct ctcaatatct tcaagaagcg tataatggcg caacacccga cggtcggcgg
     3661 cgccatcgat gtcgccgtca tcagccgcga cagcggcttt cagtggtgga agaagcaggg
     3721 cggaggctga acatggccga cacacgcaga ccggataagg cgccgcgcgg ggctttggcg
     3781 gagggtgcgg agcggttcga ccgcttggcg cgaagcgtca aggacagcca gctccgccgt
     3841 accaagacgg gcccaaggcc ggccgtcacc gagtccttcg aactcagccc ggaagatgct
     3901 tgggcaaaga agtacatgac ccattgcatc ggcccggtct gttatactgt agaggccatg
     3961 accgagccgc cggtgttcgc caaattcggg accgcgccgg aggcgtccgt ccaaggcgcc
     4021 ggagtgcagg cggaggcttc cgaaaaggcg aaaaatccgc cgccgctagg gcggaacgcg
     4081 gctgaattga gtcccatcgg cctggtaatc ggcaagcccg tgggcaagcg ttcctggctc
     4141 ggccggctgt tggggcggtg acaataagtc cgcgccggag attaatcttg tttggttcgg
     4201 cacttgcgcc ggctatggac tattgtccat gagatcagaa gagaaagcga atggagctgt
     4261 agcaatgagg cgttttgggt tccttgtgtg cgcactggtg ttctttgcgg cgttttcgtc
     4321 caaggccgcc gacaattcgc tcagcgcgga ggcgaatgcg gcctttcttg cggcgtatga
     4381 caagaagccg ggtgtcatca tcaagcccga cgggttgcgg tatcgcatcc tccacaacgg
     4441 cttcggcaga cgcccgagac cgcaggacta tgtgaccgtc aactacacgg gctcgctgat
     4501 caacggtcag gtgttcgacg gcaccgagcc gggtatgcct gcgcggttca aggccaactc
     4561 gctgatcagc ggctggaccg aagccctttc gttgatgcgt gtgggagacc gctgggaagt
     4621 ggtaatcccg ccgtcgcttg ggtacggcga acgcggcacg cccgaaggca cgataccgcc
     4681 caatcaggtg ctcgtgttcc agttggaact gatcaagacg gtgccgccga a
//
LOCUS       DASWPU010000129        10976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_62317,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000129 DASWPU010000000
VERSION     DASWPU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10976
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62317"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VGC27_02900"
     CDS             complement(<1..455)
                     /locus_tag="VGC27_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEY0281555.1"
                     /translation="MFEKTFDLQAHGTTVQREIVAGATTFFTMAYIMFVNPQILAGAG
                     MDQGAVFVATCLSAAITTLMMGLYAKLPVALAPGMGLNAYFAYSVVPALDGDWQLALG
                     CVFLSGILFVLISITPAREWLINAIPKSLKLAIAAGIGFFLALIGLANSG"
     gene            1055..2689
                     /locus_tag="VGC27_02905"
     CDS             1055..2689
                     /locus_tag="VGC27_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281556.1"
                     /translation="MRNMVSAILVLCLSVAGAHAASSWRILKDRWSEADEAGFGEFVA
                     AIGASNCSSSQSCLRDPANPYRATDQHFLDIDADCAKWPYLLRAYYAWKNGLPFSYVD
                     AVSGSGGDLRFSKTANRPAGRRDFIDRGKGIDGPRAIREVIGTVFSATYRTDAAERRG
                     VQSDFYSPALQPGSIRAGSVVYDINGHVGIVYKVDADGRIYYMDAHPDYTITRSVYGA
                     QFGQSPVRLGGGLKNWRPFELAGARRDEQGHLIGGRMVFAKNDQIPDFSLVQYLGTEP
                     NPKKDVKTARYVYNGVDLGFYEYVRVAVSGGKMDFNPLYELKAAMKTLCNDLNDRAQY
                     VDLAISQSIANRPHPSRLPDNIYGSNDGEWETYSTPSRDARIKAAAAQFHKDLADMID
                     LWVQRDERIVYDGYDLHGDLRRAYKAGSKACTITYLSSAKRPVAMTFDDMMHRLFAMS
                     FDPYHCIELRWGASGEERDSCPDSKSKLKWYEAEQRLRNQSERTYDIQMGFDLGELNR
                     HVKGTGIDEPPPVDIAALIDAMPSQVPLKGMKPVGR"
     gene            2764..2997
                     /locus_tag="VGC27_02910"
     CDS             2764..2997
                     /locus_tag="VGC27_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281557.1"
                     /translation="MPTTRVFKSGNSQAVRIPAELAYADTNIELTITRTGEVITIYPK
                     RTGSMEEIVAALRALPIPSEVETRDPIELPDRD"
     gene            3016..3402
                     /locus_tag="VGC27_02915"
     CDS             3016..3402
                     /locus_tag="VGC27_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0281558.1"
                     /translation="MLDTNIAIHARDSNGAVLDSLAKYAGQVTLSALSLVELQRGLYK
                     NPADTAIRQSRLLLLLQQVPVIAFGAVAAEAYGRIIAQCGWVKGRDYDRMIAAHAIST
                     SSVLVTANEADFRDIPGLKIENWAVV"
     gene            3486..4811
                     /locus_tag="VGC27_02920"
     CDS             3486..4811
                     /locus_tag="VGC27_02920"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF008979.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY0281559.1"
                     /translation="MRLTRYFLPTLKETPAEAQIVSHRLMLRAGLIRQEAAGIYAWLP
                     LGIRVLKKIEQIVREEQNRAGAIEILMPTLQQAELWQESGRYDAYGKEMLRIEDRHER
                     KLLYGPTNEEMVTEIFRSTVRSYKALPLNLYHIQWKFRDEVRPRFGVMRGREFLMKDA
                     YSFDLDKAGAKKSYNKMYVAYLRTFARMGLQSIPMRAETGPIGGDLSHEFIVLADTGE
                     SGVFCHKDLLDMPIPGDDVDFDGDLEPIVARRSALYAATEDVHEPQRFEAEVPADKRL
                     SARGIEVGQIFYFGAKYSKPMKAVVAGPDGREVPVEMGSYGVGVSRLVGAIIEASHDD
                     AGIVWPEPVAPFDIGLVNLKAGDAKTDAACEDLYARLTKAGKDVLYDDTAERPGAKFA
                     DMDLIGLPWQVIVGPRGLEKGLVEVKNRKTGAKEEMSADAVVNRLDAGT"
     gene            4840..5148
                     /locus_tag="VGC27_02925"
     CDS             4840..5148
                     /locus_tag="VGC27_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0281560.1"
                     /translation="MFRLVETETFSKWLTALESRGRARIQRRLDRLALGNPGDVKPVG
                     KGVSEMRIDYGPGYRVYYFRRGREIIFLLAGGDKRSQRRDIELAIAIKEVVKDISWPM
                     "
     gene            5136..5438
                     /locus_tag="VGC27_02930"
     CDS             5136..5438
                     /locus_tag="VGC27_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEY0281561.1"
                     /translation="MANVTKVKLRPYDSAAYLKTGEDVANYLEAVFEDGDPALIAHAL
                     GVVARSKGMAKIAKKTGLGRESLYKALSREGNPELLTVLKVVHALGLRLTVTADAA"
     gene            5435..6697
                     /locus_tag="VGC27_02935"
     CDS             5435..6697
                     /locus_tag="VGC27_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY0281562.1"
                     /translation="MKNSTRPFAPFEWMLALRYLRAKRAESFISVISMISLVGIALGV
                     ATLIIVMAVMNGFRHELFSRILGLQGHAVVQGIEGSLPNFDRAAARVRSVPGVVRVAP
                     IVDGQAIASSGGSTTGVLVRGLRQTDLKQLTTVSKTLSAGALDRYEGGEAVIVGNRLA
                     QKLRIVPGMKVTLMMPRGDVTPFGMTPRIKTYTVAGTFNIGMSEYDQTFVFMPLAESQ
                     LFFNTGEAVSGLETMVADPDKIDAMAAPIARAAGPYARVVTWKDMNSTFFSAIETERN
                     VMFLILTLIILVAALNIVSGLYMLVKDKSADIAILRTMGATRGAIMRVFLIAGLSIGM
                     IGTFLGFVIGVLFCDNIESIRQFLSALTGTALFNPEIYFLSRMPAEMDPAEVAAVVLM
                     SLALTFLATLYPSWRAARLDPVEALRYE"
     gene            6690..7406
                     /locus_tag="VGC27_02940"
     CDS             6690..7406
                     /locus_tag="VGC27_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0281563.1"
                     /translation="MSNPFAVAALRLDMVARTYREGEGQLEVFSGVNLSLAAGELVAL
                     VGPSGAGKSSLLHMAGLLEAPSAGEIVIAGAPVSSLTEAERTRIRRETIGFVYQAHHL
                     LPEFTALENVVIPQLIAGKRRKDADVEAARLLTLMGLENRLTHRPSQLSGGEQQRVAI
                     ARALANKPRLLLADEPTGNLDPRTAAGVFEALIAIVRSEGLAVLVATHNLALAAKMDR
                     ALILHEGRLIDGGKLGTQPI"
     gene            7462..8085
                     /locus_tag="VGC27_02945"
     CDS             7462..8085
                     /locus_tag="VGC27_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY0281564.1"
                     /translation="MNPQSLLLSLLAVDLLAVVSPGPAFVIVSQMSVRHGARSGIAAV
                     SGIVVAVWFWCAVVLSGLTILFQIAPWFYGAMKLAGGAYLIYLGLNLLCARAGDGAGE
                     PLGGTELSMAKGFWKGLLLGLTNPKAVVYFGSIFTLFVKPGAPVWLQAAAVGIATFDT
                     LAWYGLVGVLFSHSAVRRAYGRVRHWIERAAGAVMVAFGLRLVLAKD"
     gene            8378..8821
                     /locus_tag="VGC27_02950"
     CDS             8378..8821
                     /locus_tag="VGC27_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HEY0281565.1"
                     /translation="MVVTKKNKASAKKKPDVKPPSDAELKALLGSASSVWSAVVLAVK
                     DSFAPSQLEWLPSKQPFGRAGRIRHKGRTLLYLLPGDGKFDVAVVLGERAVELAMAAP
                     LPAAMKKRIAGAKRYVEGRSIRFPVNSASEVAVVVKLVELKTTPK"
     gene            8832..>10976
                     /gene="dnaE"
                     /locus_tag="VGC27_02955"
     CDS             8832..>10976
                     /gene="dnaE"
                     /locus_tag="VGC27_02955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY0281566.1"
                     /translation="MAEAKKPAGFVHLRVKSAYSLLEGAVRPGELAKLTRDGAMPAVA
                     VTDVNNLFGVFEITETLAKAGVQPIVGCLLAVELGRAAGGHGSRGKPPLLPLLVQNEA
                     GYLNLTKLLSAAYLGAEPGDWPHVTAAALAEHAEGLIALTGGPGGPLNRLIVEGQPEA
                     GAALLDALAAIYPGRLYVELQRHGLAEERAAEDGLIDLAYAKALPLVATNDVHFGPAG
                     MYEAHDALLCIADGAFISQDDRRRLTREHRFKSAAEMAAQFADLPEAIENTIEIARRC
                     AFRPKRRDPILPQFVPESGRTPEDEIRVQAEAGLARRLARHGRHAEEKTYRDRLQFEL
                     GIIIKMQFAGYFLIVSDFMKWTRAQGIPVGVRGSGATSLVAWALDITNLDPIRFGLLF
                     ERFLNPERISMPDFDIDFCQERRDEVVRYVRDKYGHDKVAHIIALGSLQARAAVRDVG
                     RVLQMPLGLVDRIAKLIPNPPGRHVELQDAMEGEPRLQQIAEQEPMAQRLFSIVGKIE
                     GLYRHASTHPAGVVIGDRPLDELLPLYRDPRSEIPVTQFDYEDAEKAGLVKFDFLGLK
                     TLTVISKAEALLRRRGISLDTQNIDFDDRPTFDMLARGDSVGVFQLEGAGMRDLMRKM
                     KPDHINDLVALVALYRPGPMDSIPKYIAVKNGKEAPEYLHPALEPILKETYGVMTYQE
                     DVMNIARELAGYSLGEADLLRRAMGKKIASVMA"
BASE COUNT         1997 a         3423 c         3550 g         2006 t
ORIGIN      
        1 cccgaattgg ccagcccgat cagggcgagg aagaatccga tccccgccgc gattgccagc
       61 ttcaggcttt tcggtattgc gttgatcagc cactcgcgcg cgggcgtgat cgagatcagc
      121 acgaacagga tgccggagag gaagacgcag cccagcgcca attgccagtc gccgtccagc
      181 gcgggcacga cgctgtaggc gaaataggcg ttcagcccca tgcccggcgc gagcgccacg
      241 ggcagcttgg cgtacagacc catcatcagc gtcgtgatcg cggccgagag gcaggtcgcc
      301 acgaacaccg cgccctggtc catgcccgcg cccgcaagaa tctgcgggtt gacgaacatg
      361 atgtaggcca tggtgaagaa ggttgtggcg ccggccacga tctcgcgctg gaccgtcgtg
      421 ccatgcgctt gcagatcgaa cgtcttctcg aacatctccg ccccccgaac cagcgaggcg
      481 gtaaaatagc gaggaagagg gcaaagccaa aatgtgaaag gccgggcgaa acccggcctt
      541 tgcaccgcag atccctgcgt agttctcccc gaacttggcc gttcttatat agaggccttg
      601 accgtccgtt tatcctcgtt accgataatg gaatttcgtg aaaatgtcac gattatttgg
      661 ggtgcgtgca atgataagaa gggcggaaac gggcggaata gctaccctgt tggttttgtt
      721 agaattggtt gcgctaccat acgcgaacgc ccggccgcgg acggcggaag acagcgctgt
      781 caatagtggc gaaacagaca cgtgcgcgcc caatcttgcg ggagccggct atgtcggcgg
      841 agtggatgtc gagggcaacc gaatcacccc ggcggatatt ggcggcggga ccagtgtcga
      901 tctgggccgg atttccgcta ctcccgtgct ggtgcccggt cgtcatggcc ggcaggcaga
      961 cgtcgtcgtc cagggcgcgg aaccggccaa gcccccctgc gaacggccgc cccattaact
     1021 tgacttcgcc gcgctctccc aggagcgtgg gcgcatgcgc aatatggtct cagcgatcct
     1081 tgttttgtgc ctgagtgttg cgggcgcgca cgcggccagc tcctggcgca ttctcaagga
     1141 ccgttggtcg gaggcggacg aggcgggctt cggtgagttc gtcgccgcca tcggcgccag
     1201 caattgcagc tcgtcgcaaa gctgtctgcg cgatccggcc aatccctacc gcgccactga
     1261 ccagcatttc ctcgacatcg acgccgattg cgccaaatgg ccgtatctgc tgcgcgccta
     1321 ctatgcctgg aagaacggcc tgccgttttc ctatgtcgat gcggtgagcg ggtccggcgg
     1381 cgacctgcgc ttcagcaaga cggccaatcg cccggccgga cgccgcgact tcatcgaccg
     1441 cggcaagggc atcgacggac cgcgcgccat ccgcgaggtg atcggcacgg tgttctccgc
     1501 cacctaccgc accgatgcgg cggagcggcg cggcgtgcag tcggatttct attcgccggc
     1561 gttgcagccg ggaagcatcc gcgcgggcag cgtggtctac gacatcaacg gccatgtcgg
     1621 catcgtctat aaggtcgatg cggacgggcg catctactac atggacgccc atcccgatta
     1681 caccatcacc cgcagcgttt acggcgccca gttcggccag agtccggtgc ggctgggcgg
     1741 cggcctcaag aactggcggc cgttcgagct ggcgggcgcg cgccgggacg agcagggcca
     1801 tctcatcggc ggacgtatgg tcttcgccaa gaacgaccag attcccgatt tctcgctggt
     1861 ccagtatctc ggcaccgaac ccaatcccaa gaaagacgtg aagacggcgc ggtacgtcta
     1921 taacggcgtg gaccttggct tctacgaata tgtgcgcgtg gcggtgtcgg gcgggaagat
     1981 ggacttcaat ccgctctacg aactcaaggc cgcgatgaag acgctgtgca acgacctgaa
     2041 cgatcgcgcg caatatgtcg atctcgccat ctcgcagagc atcgcgaacc ggccgcatcc
     2101 ctcgcgcctg cccgacaaca tctacggctc caacgacggc gaatgggaga cctattccac
     2161 gccctcgcgc gacgcgcgca tcaaggcggc cgccgcgcag ttccacaagg accttgcgga
     2221 catgatcgat ctgtgggtcc agcgcgacga gcgcatcgtc tatgacggct acgacctgca
     2281 cggggatttg cgccgggcct acaaggccgg atcgaaggcc tgcaccatca cctatctcag
     2341 ttccgccaag cggccggtgg cgatgacgtt cgacgacatg atgcaccggc tgttcgccat
     2401 gtcgttcgat ccttaccact gcatcgaact gcgctggggc gccagcgggg aggagcgcga
     2461 ctcctgcccg gattccaagt cgaaattgaa atggtacgag gccgagcagc gcctcaggaa
     2521 ccagtccgag cgcacctacg acatccagat gggcttcgac ctcggcgagt tgaaccggca
     2581 tgtgaagggc acgggcatcg acgagccccc gccggtggat atcgcggcgc tgatcgacgc
     2641 catgccttcc caggtgccgt tgaaaggaat gaagccggtg ggacggtgat gtatatacgc
     2701 gtatttacat catgcgatgt ctgtgtcaga atgcatcgaa tttgctgagt gtgagagcag
     2761 tctatgccta cgacacgtgt ctttaaatca ggcaatagcc aagcggtgcg cattcccgcc
     2821 gagcttgcct atgccgacac gaacatcgag ctaacgatca cccgcacggg tgaagtcatc
     2881 acgatttacc cgaagcgtac aggcagcatg gaagaaatcg ttgctgcgct tcgggcgttg
     2941 cctataccct ccgaagtaga gacgcgcgac cccatcgagt tgcctgaccg cgattaggac
     3001 cgatccttgg cttatatgct ggatacgaac atcgcgattc acgcgcgcga cagcaacggc
     3061 gcggtgctcg acagtcttgc caagtatgcc ggacaagtaa cgctttccgc gttgagcctc
     3121 gtggaattgc agcgcggact ctataagaat ccggccgaca cggcgatacg tcaaagccgc
     3181 cttttgctgc tgctgcagca agtcccggtc atcgccttcg gtgcggttgc cgccgaagct
     3241 tatggccgga tcatcgccca atgcggctgg gtcaaagggc gcgactacga ccggatgatc
     3301 gcggcgcacg ccatcagcac gtcgtcggtc ctcgtcaccg cgaacgaagc cgattttcgc
     3361 gacatccctg gcctcaagat cgagaactgg gctgtcgtct gaggcctcca agcgcggtct
     3421 cccggcaatt gcccccggcc ccagggttcc ttatgaaggg ggcgttcaac tgcttcggaa
     3481 ccgctatgcg cctcacccgc tatttcctgc ccaccctcaa agaaaccccc gccgaggcgc
     3541 agatcgtttc gcaccggctg atgctgcgcg ccggcctgat ccgccaggag gccgccggca
     3601 tctatgcctg gctgcccttg ggcatccggg tgctgaagaa gatcgagcag atcgtgcgcg
     3661 aggaacagaa ccgcgccggg gccatcgaaa tcctgatgcc gaccctgcaa caggccgagt
     3721 tgtggcagga gagcgggcgc tacgacgcct acggcaagga gatgctgcgc atcgaggacc
     3781 gccacgagcg caagctgctc tacggcccca ccaacgagga gatggtgacg gaaatcttcc
     3841 gctccactgt gcgctcgtac aaggcgctgc cgctcaacct ttatcacatc cagtggaaat
     3901 tccgcgacga agtgcgcccg cgtttcggcg tcatgcgcgg ccgcgagttc ctgatgaagg
     3961 acgcttattc gttcgatctc gacaaggcgg gcgccaagaa atcctacaac aagatgtatg
     4021 tcgcttacct gcggactttc gcgcggatgg ggttgcagtc gattccgatg cgtgccgaga
     4081 ccggccccat cggcggcgat ctctcccacg agttcatcgt gctcgcggac accggggaga
     4141 gcggcgtgtt ctgccacaag gatctgctcg acatgccgat ccccggcgac gatgtggatt
     4201 tcgacggcga tctggagccg atcgtcgccc ggcgctccgc gctctacgcc gcgaccgaag
     4261 acgtccacga gccgcagcgt ttcgaggcgg aagtgccggc ggacaaacgg ctgtccgcgc
     4321 gcggcatcga ggtcggccag attttctatt tcggcgccaa atactccaag ccgatgaaag
     4381 ccgtggtggc gggccccgac ggcagggaag tgccggtgga gatggggtct tacggcgtcg
     4441 gcgtgtcgcg gctggtcggc gccatcatcg aagcctcgca tgacgatgcc ggaatcgtct
     4501 ggccggagcc ggtggcgccg ttcgatatcg gcctcgtcaa cctcaaggcg ggcgacgcca
     4561 agacggacgc cgcctgcgaa gacctttacg cgcggctgac gaaagcgggc aaggacgtgc
     4621 tctacgacga caccgccgaa cgtccggggg cgaaattcgc cgacatggat ttgatcggtc
     4681 ttccctggca ggtgatcgtc ggcccgcgcg ggctggagaa gggcttggtg gaggtcaaga
     4741 accgcaagac cggcgccaaa gaggaaatgt ccgcggacgc ggtcgtgaac cggctcgacg
     4801 ccggaaccta gttgacatct tgtatcctac agaatacaca tgttccggct cgtagaaact
     4861 gaaacctttt cgaaatggct gacggcgttg gaaagccgag gccgcgcccg tattcagcgt
     4921 cggttggatc gtttggcact gggaaatccc ggagacgtga agccggtcgg caaaggggtc
     4981 tcggagatgc ggatcgatta cggaccaggc taccgtgtct attactttcg gcgtggtcgg
     5041 gagatcatat ttctcttggc cggcggcgat aaacggagcc agcggcgcga tatcgagttg
     5101 gcgatcgcaa tcaaggaagt cgtaaaggac atttcatggc caatgtgacg aaggtgaagc
     5161 tgcggcccta tgattcggct gcgtatctca agaccggaga agacgttgcg aactatctgg
     5221 aagccgtctt tgaagacggc gatccggcac tgatcgcgca cgctctgggt gttgtggcgc
     5281 gatcgaaagg catggcgaag atcgccaaaa agacggggct gggtcgcgaa agcctctata
     5341 aggcgttgtc gcgtgaaggg aatccggagc ttctgaccgt gctcaaagtc gtccacgcat
     5401 tgggtcttcg cctcaccgta acggcggacg cagcgtgaaa aattccaccc gccctttcgc
     5461 gccgttcgag tggatgctgg cgctgcgcta cctgcgcgcc aagcgggcgg aaagcttcat
     5521 ctcggtcatt tcgatgattt cgctggtcgg catcgcgctc ggcgtcgcca cgctgatcat
     5581 cgtgatggcg gtgatgaacg gcttccgcca cgagctgttt tcgcgcatcc tcggtttgca
     5641 ggggcacgcc gtggtgcaag gcatcgaagg ctcgctgccg aatttcgacc gcgccgcggc
     5701 gcgggtgcga tcggtgccgg gcgtggtgcg cgtggcgccc atcgtggacg ggcaggcgat
     5761 cgcaagctcc ggcggcagca ccacgggcgt gctggtgcgc ggcctgcgcc agaccgacct
     5821 gaaacagttg acgaccgttt ccaaaacgct gtcggcgggc gcgctcgatc gctacgaggg
     5881 cggcgaggcg gtgatcgtcg gcaaccggct ggcgcagaag ctcaggatcg tgcccggcat
     5941 gaaggtcacg ctgatgatgc cgcgcggcga cgtcaccccg ttcggcatga ccccgcggat
     6001 caagacctac accgtcgccg gcaccttcaa catcggcatg agcgaatacg atcagacctt
     6061 cgtgttcatg ccgctggcgg aatcgcagct cttcttcaac accggcgagg cggtcagcgg
     6121 cctggaaacg atggtcgccg atcccgacaa gatcgacgcc atggccgcac ccatcgcgcg
     6181 cgcggccggg ccttacgcgc gcgtggtcac ctggaaggac atgaattcga ccttcttcag
     6241 cgccatcgag accgaacgca acgtgatgtt cctgatcctg acgctgatca tcctggtggc
     6301 ggcgctgaac atcgtctccg gcctttacat gttggtgaag gacaaatcgg cggacatcgc
     6361 catcctgcgc accatgggcg ccacgcgcgg ggcgatcatg cgcgtgttcc tgatcgccgg
     6421 cctcagcatc ggcatgatcg ggacgttcct cggtttcgtc atcggcgtgc tgttctgcga
     6481 caatatcgaa agcatccgcc agtttctctc cgcactgacc ggcaccgcgc tgttcaatcc
     6541 ggaaatctat ttcctcagcc ggatgccggc cgaaatggac cccgccgagg ttgccgccgt
     6601 cgtgctgatg tcgctggcgc tcaccttcct ggcgacgctc tatccttcct ggcgggcggc
     6661 gcggctcgat ccggtggagg cgctgcgcta tgagtaaccc cttcgccgtt gccgctctgc
     6721 gcctggacat ggtcgcccgc acttaccggg aaggcgaggg ccagcttgaa gtcttcagcg
     6781 gcgtgaacct gtcgctcgcg gcgggcgaac tggtggcgct ggtgggaccg tcgggcgcgg
     6841 gaaaatcctc gctgctgcac atggccggcc ttctggaagc gccgagcgcg ggcgagatcg
     6901 tgatcgcggg cgcgcccgtc tcctcgctca ccgaagccga gcgcacccgc atccgccgcg
     6961 agaccatcgg cttcgtctat caggcgcacc atctgctgcc ggaattcacc gcgctggaaa
     7021 acgtcgtcat tccgcagctc atcgccggca agcgccgcaa ggacgccgat gtggaagccg
     7081 cgcgcctgtt gaccttgatg gggctggaaa accgcttgac ccatcgcccg tcgcagcttt
     7141 ccggcggcga gcagcaacgc gtggcgatcg cccgcgcgct ggcgaacaag ccgcggctgc
     7201 tgctggccga cgagcccacc ggcaatctcg atccgcgcac cgcggcgggc gtgttcgagg
     7261 cgctgatcgc catcgtgcgc tccgaaggat tggcggtgct ggtcgccacc cacaatctgg
     7321 cgctggcggc caagatggac cgcgccctga tcctgcacga aggccggctg atcgacggcg
     7381 gcaagctcgg tacccagcct atatgaaccg ctcttgacgg atgtgttagg ctgctttgta
     7441 gagagaaatt cagaaaaatc gatgaaccca cagtcgcttc tcctgtccct gctggcggtg
     7501 gatttgctgg ccgtcgtcag ccccggtccg gcctttgtga tagtgtcgca gatgtccgtt
     7561 cgccacggcg cacgatccgg catcgccgcc gtctcaggta ttgtcgtagc cgtatggttt
     7621 tggtgcgccg tcgtcctttc cgggctgacg attttattcc agatcgcgcc ttggttttat
     7681 ggcgccatga aactcgcggg cggcgcctac ctgatttatc tcggactgaa cctgttgtgt
     7741 gcgcgggcgg gcgacggcgc cggcgaaccg ctgggcggaa cggaactctc catggcaaaa
     7801 ggtttttgga aggggctcct gcttggcctg accaatccga aagcggtggt ttatttcggc
     7861 agcatcttca cactgttcgt gaaaccgggc gcgcccgtct ggttgcaggc ggctgcggtc
     7921 ggcatcgcaa ccttcgacac gctggcctgg tatggcctgg tcggcgtgct gttctcgcac
     7981 tccgccgtcc ggcgggccta tggccgcgtc cgtcactgga tcgagcgcgc cgccggggcg
     8041 gtgatggtcg cgttcggact gcggcttgtt ctcgccaagg attagccgcc gttttcaggc
     8101 ttgccgcttt gatcccgctt tgttcttgac agaacgaata aagaacgttt acggtacttc
     8161 tccaacgacg gagagtatta tggcttggtt caaggatgcg gcggcggggg tcgggctggt
     8221 ggtcattctg gtctcgtcct ttgtcctgac cagcgcggcg caggcgtttt tcgccgccgg
     8281 gtgaagttgc tgtggatgga tggcacgccg ccctcgcgta gggcgcgcgg cggtcgctac
     8341 acccgtgtgc ggcggttttc tcgcggcagg agggtcgatg gtcgtcacca agaagaacaa
     8401 ggcatctgcc aagaaaaagc cggatgtgaa gccgccctcg gacgccgaat tgaaagctct
     8461 gttaggcagt gcaagctctg tttggtcggc ggtcgtcctg gcggtcaagg acagcttcgc
     8521 tccgtcgcaa ctggagtggc ttccgtcgaa gcaacccttc gggcgggcag gccggatacg
     8581 gcacaaggga cggacgctgc tttacctgct tccgggcgac ggtaagttcg atgtggccgt
     8641 ggttttgggc gaaagggccg tggaacttgc catggcggcc ccgctgcccg ctgcgatgaa
     8701 gaagaggatt gccggagcga agcgctatgt ggaaggacgc agtatccggt tcccggtgaa
     8761 ttccgcaagc gaggttgccg tcgtcgtgaa gctggtagag cttaagacga caccgaagtg
     8821 aaggggcatt gatggctgag gcgaagaagc ctgccgggtt cgtgcatctt cgggtgaaga
     8881 gcgcctattc gctgctggag ggcgctgtgc gccccggcga actggcgaag ctaacccgcg
     8941 acggcgcaat gccggcggta gcggtcaccg acgtcaacaa cctgttcggt gtcttcgaaa
     9001 tcaccgagac cctagccaag gcgggcgtac agcccatcgt gggctgcctg ctggcggtcg
     9061 aactgggaag ggcggcgggc gggcacggct cgcgcggcaa gccgccgctg ctgccgctgc
     9121 tggtgcaaaa cgaagccggc tacctcaatc tgacgaaact cctcagcgcc gcctatctcg
     9181 gcgccgagcc gggcgactgg ccgcatgtga cggcggcggc tctggcggag catgcggagg
     9241 ggctgatcgc gctcaccggc gggccgggag ggccgctcaa ccgcctgatc gtcgaaggcc
     9301 agcccgaggc cggcgcggcc ctgctcgatg ctctggccgc gatttacccc ggacgccttt
     9361 atgtggagtt gcagcgccac ggtctggccg aggagcgcgc cgccgaggac ggactgatcg
     9421 atcttgccta cgccaaggcg ctgcctctgg tggcgaccaa cgacgtgcat ttcggccccg
     9481 ccggcatgta cgaggcgcac gacgcgctgc tctgcatcgc cgacggcgct ttcatctcgc
     9541 aggacgaccg caggcggctg acccgcgagc accgcttcaa gtcggcggcc gaaatggcgg
     9601 cgcagttcgc cgacctgccc gaagcgatcg aaaatacgat cgagatcgcg cggcgctgcg
     9661 ccttccgtcc caagcggcgc gaccccatcc tgccgcaatt cgtaccggag tccgggcgca
     9721 cgcccgaaga cgaaattcgc gtccaggccg aggccggtct ggcgcggcgc ctggcgcggc
     9781 acggccgcca tgccgaggaa aagacctatc gcgaccggct gcaattcgag ctcggcatca
     9841 tcatcaagat gcagttcgcg ggctatttcc tgatcgtttc cgacttcatg aaatggacgc
     9901 gggcgcaggg cattccggtg ggcgtgcgcg gctcgggcgc gacctcgctg gtcgcctggg
     9961 cgctggacat caccaacctc gatcccatcc gcttcggcct cttgttcgag cgcttcctca
    10021 atcccgagcg catctcgatg ccggacttcg atatcgattt ctgccaggag cggcgcgacg
    10081 aagtggtgcg ctacgtgcgc gacaaatacg gccacgacaa ggtggcgcac atcatcgcgc
    10141 tggggtcgct gcaggcgcgc gccgcggtgc gcgacgtggg gcgcgtgctg cagatgccgc
    10201 tgggcctggt cgaccgcatc gccaagctga tccccaatcc gcccggccgc catgtcgagc
    10261 ttcaggacgc catggagggc gagccgcggc tgcaacagat cgccgagcag gagccgatgg
    10321 cccagcgcct gttttccatc gtcggcaaga tcgaagggct ttaccgccac gcctccaccc
    10381 atcccgccgg cgtggtgatc ggcgaccgcc cgctggacga gctgctgccg ctgtatcgcg
    10441 atccgcgctc ggagattccg gtcacccagt tcgattacga ggacgctgaa aaggccggct
    10501 tggtcaagtt cgatttcctg ggtttgaaaa cgctgacggt catctccaag gcggaggcgc
    10561 tgctgaggcg gcgcggcatc tcgctcgata cccagaacat cgatttcgac gaccgcccca
    10621 ccttcgacat gctggcgcgc ggcgacagcg tgggcgtgtt ccagctcgaa ggcgcgggca
    10681 tgcgtgacct gatgcgcaag atgaagcccg atcacatcaa cgatctggtc gcgctggtgg
    10741 cgctctatcg ccccggcccg atggattcga ttcccaaata catcgccgtg aagaacggca
    10801 aggaggcgcc cgaatacctt catcccgcgc tggagccgat cctgaaggaa acctacgggg
    10861 tgatgaccta tcaggaagac gtgatgaaca tcgcccgcga gttggcgggc tattcgctcg
    10921 gcgaggcgga tctcctgcgc cgcgcgatgg gcaagaagat cgcctccgtg atggcc
//
LOCUS       DASWPU010000130         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_49896,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000130 DASWPU010000000
VERSION     DASWPU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49896"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VGC27_02960"
     CDS             complement(<1..349)
                     /locus_tag="VGC27_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEY0281567.1"
                     /translation="MQEAGIKPEDVLALMNEPACAHNAKAKSGCAKPTPGATQGGCCF
                     DGARNALLPIADVAHLVHGPIGCTGSSWDNRGTRSSGIDTYRIGMTTDLNEMDVILGR
                     GGKRLFQAIRQAVE"
     gene            721..>2168
                     /locus_tag="VGC27_02965"
     CDS             721..>2168
                     /locus_tag="VGC27_02965"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0281568.1"
                     /translation="MDMNNTITIDVGDFAEEAIEDFTHCFTGECRVSVLPILFQISQI
                     ITDNEDLVASLAVIFKVMQERLKIQRAMVTLYDRLSEANFIHESFGLSDEQRSDGIFS
                     LNDGIAAKVIETGKAIIVPPRRTALGQTDRSVANGIPANASFFCVPIMHAKKVLGTIS
                     AERVYHNRRLLKQDVQLLATIASMIAPAVELYLLENVEKVRLENENSQLKNALKERFK
                     PSNIIGNAKPMQEIYELIHKVAATKATVLILGESGVGKELVASAIHYNSPSANGPFVK
                     FNCAALPENLVESELFGHEKGAFTGAIGTRSGRFEQADGGTLFLDEVGELSLPIQAKL
                     LRVLQEKTFERIGGNRPVKVDLRIIAATNRDLTGMIANGSFREDLYYRLNVFPMTIPP
                     LRDRGSDVILLADHFVAYYAGEIGKEVKRISTPALNMLMAYSWPGNVRELENVIERSV
                     ILSEDGVVHGYNLPPSLQTSAETGTSFGCGLEA"
BASE COUNT          461 a          641 c          638 g          428 t
ORIGIN      
        1 tttcgaccgc ctggcgaatg gcctggaaca gacgtttgcc gccgcgcccc aggatgacgt
       61 ccatttcgtt gagatcggtg gtcatgccga tgcgataggt gtcgataccg ctcgaccgcg
      121 tgccgcggtt atcccaggac gatccggtac agccgatcgg gccgtgaacc agatgggcga
      181 catcggcaat cggcagcagg gcattgcggg ccccatcgaa gcagcatccg ccttgggtcg
      241 cgccgggcgt cggtttggcg caaccggact tggccttggc gttgtgggcg caagccggct
      301 cgttcatcaa ggcaagaacg tcttcgggtt ttatgcccgc ctcctgcatt ggcagcgttc
      361 ccgtttcccg aagcaatcgg cgggccaaac gccggacggc gcgcgaagtt tccgccaatg
      421 acgcatccgg cctggaaagc gaccgggccg ggccgtggaa acgtacgttt cgggcgcgag
      481 aggggaaaat tttgtacggt tgcgaacatt gtcgtttgtc gcgcaaagac gacgccggca
      541 aacaccgcat tctccgataa gttacaataa cgtacaaaat gtcgctttgc cgccaatcgc
      601 gccggcgccg ccgctttagg ttggcaataa tatactataa aatcaatcgg ttatggcgca
      661 tccgccgtgg ccctgaattt gcaatgtccg gtttggcctt cggtgtccga ggtgaatcgg
      721 atggatatga acaatacgat taccatcgat gtcggcgact ttgccgaaga ggcgatcgag
      781 gacttcaccc attgctttac cggcgaatgc cgggtcagcg tgctgccgat tctgtttcag
      841 atcagccaga tcatcaccga taacgaggat ctggtggcct cgcttgccgt cattttcaag
      901 gtgatgcagg agcggttgaa gattcagcgg gcgatggtga cgctgtacga ccgtctgtcg
      961 gaagccaatt tcatccatga gagtttcggt ctttcggatg aacagcggtc ggacgggata
     1021 ttttccttga acgacggcat cgccgccaaa gtcatcgaga ccggaaaggc gatcatcgtt
     1081 ccgccgcggc gcacggcgct cggccaaacg gatcgctccg tcgccaacgg cataccggcg
     1141 aatgccagct ttttctgcgt acccatcatg catgcgaaaa aggtgctggg aaccatcagc
     1201 gcggaacggg tctatcacaa ccgccggctg ctcaagcagg atgtacaatt gcttgccacc
     1261 atcgcctcga tgatcgcccc ggcggtggaa ctttatctgc tcgaaaatgt cgagaaggtc
     1321 aggctggaga acgagaacag ccagctcaaa aacgccctga aagagcgctt caagccgtcc
     1381 aacatcatcg gcaacgccaa gcccatgcag gaaatctatg agctgatcca caaggttgcc
     1441 gccaccaagg cgacggtcct gatcctgggc gaaagcggcg tcggcaagga gctggttgcc
     1501 agcgccattc actacaatag cccgagcgcc aacggcccct tcgtcaagtt caactgcgcc
     1561 gcgctgccgg aaaatctggt cgagagcgaa ttgttcgggc atgagaaggg cgccttcacc
     1621 ggcgccatcg gaacgcgcag cggccggttc gaacaagccg acggcggcac cctcttcctc
     1681 gacgaggtcg gagaacttag tttgccgatc caggcgaaac tgttgcgggt gttgcaggaa
     1741 aaaaccttcg agcggatcgg cggcaatcgt ccggtcaagg tcgatttgcg catcattgcc
     1801 gcaaccaacc gcgacctgac gggcatgatc gcaaacggca gtttccgcga ggacctttac
     1861 tatcgcctca acgtctttcc gatgacgatc ccgcccttgc gcgatcgcgg cagcgacgtg
     1921 atcctgctcg ccgatcattt tgtcgcctat tacgccggag aaatcggcaa agaggtcaaa
     1981 cgcatatcga ccccggcgct caacatgctg atggcttatt cctggcccgg caacgtccgc
     2041 gaactcgaga acgtcatcga acgctcggtc attttgtcgg aagacggagt cgttcacggc
     2101 tataatctgc cgccgtcgct gcagacctcc gccgagaccg gcaccagctt cggctgcggc
     2161 ctggaagc
//
LOCUS       DASWPU010000131         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_25394,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000131 DASWPU010000000
VERSION     DASWPU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25394"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1126)
                     /locus_tag="VGC27_02970"
     CDS             complement(113..1126)
                     /locus_tag="VGC27_02970"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY0281569.1"
                     /translation="MDNLRTATIRSTGHHRLHEVQSSAQAVTARPLHLHRRMSLEDAF
                     RTTVLECLAHVAANIAPVLRARDMEGLHQLRVGLRRLHVAFSAFGEEFRTPALMDLRA
                     RTKAFADAVAPARDLDVFIEELLAPMAGRLGHDESVKVLLARAEEARRSAWDEAAAHI
                     ASTEFDVFQDDAAAAAEARSWLDGRDAVDLKARLAVRVPVKPAAERLMEEQLIRARKR
                     GRRLKTLEDREFHRTRIALKRLRYAAEFYGPLFKKKAVKRYLDRMKKLQDLLGRINDA
                     AQVRSVLARLLADETLSAHIQADLYFTAGLINGWHRARAERLCKKALRRWDKFKRQEP
                     FWA"
     gene            complement(1146..1907)
                     /gene="hisF"
                     /locus_tag="VGC27_02975"
     CDS             complement(1146..1907)
                     /gene="hisF"
                     /locus_tag="VGC27_02975"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017395071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY0281570.1"
                     /translation="MLKIRIIPCLDVKDGRVVKGINFVGLRDAGDPVEQAMLYDKMGA
                     DELCFLDITASHEKRNILLDVVRRTADVCFMPLTVGGGVRTLDDIRALLLAGADKVSV
                     NTEAVRRPEFIREGAEKFGSQCIVAAIDAKGVAPGRFEVFTHGGRTPTGLDAVAHAVK
                     LVEYGAGEILLTSMDRDGTKIGYDIALTRAIADAVAVPVIASGGVGSLEHLVEGVRDG
                     HANAVLAASIFHFGQWSIAEAKTALAKAGLPVRNV"
BASE COUNT          329 a          645 c          653 g          349 t
ORIGIN      
        1 ttttgacagc caacccgttt tcaactgggt ggcccgcatg gagtttaagc gccggccgcg
       61 ctttgcgcgg accgggggcg ggccatgaca agttaggatt agtgttgtca attcacgccc
      121 agaacggttc ctggcgtttg aacttgtccc agcggcgcaa cgcctttttg cataagcgtt
      181 cggcacgggc gcggtgccag ccgttgatca gtccggcggt gaaatagagg tcggcctgga
      241 tatgcgccga cagtgtttcg tcggcgagaa ggcgtgccag cacgctgcgc acctgcgccg
      301 cgtcgttgat gcggccgaga aggtcttgaa gcttcttcat gcggtcgaga tagcgcttca
      361 ccgccttctt cttgaacagc gggccgtaga attccgccgc ataacgcagc cgcttgagcg
      421 cgatgcgggt gcgatggaat tcgcggtcct cgagggtctt caagcgccgg ccgcgcttgc
      481 gcgcccggat cagctgctcc tccatcagcc gctccgccgc cggtttgacc ggaacgcgca
      541 ccgccagccg cgctttcaga tcgacggcgt cccgcccgtc cagccaggaa cgcgcctcgg
      601 cggcggccgc ggcgtcgtcc tgaaagacgt cgaattcggt gctggcgata tgcgccgccg
      661 cttcgtccca ggcgctccgg cgcgcctctt cggcgcgggc gagcaaaacc ttgactgatt
      721 cgtcatggcc gaggcggccc gccatcggcg ccagcaattc ctcgatgaaa acgtcgagat
      781 cgcgcgccgg cgccacggcg tcggcgaaag ccttggtgcg cgccctcaaa tccatcagcg
      841 cgggggtgcg gaactcttcg ccgaaggcgg aaaacgccac atgcagccgg cgcaggccga
      901 cgcgcaattg atgcaggccc tccatgtcgc gcgcgcgcag cacgggcgcg atgttggcgg
      961 cgacatgggc caggcattcg agcacggtgg tgcggaacgc atcctccagg ctcatgcgcc
     1021 ggtgcaaatg cagcggccgc gccgtaaccg cttgggccga gctttgaacc tcgtgcagtc
     1081 tgtgatggcc cgtcgagcgg atcgtcgcgg tgcgaagatt atccatcggg gaactccggc
     1141 gcaggtcaaa cattgcgcac gggcaaaccg gctttggcaa gagcggtctt ggcctcggcg
     1201 atagaccatt ggccgaaatg gaagatcgat gcggccagca ccgcattggc gtggccgtcg
     1261 cgcacgccct ccaccaaatg ttcgaggctg cccaccccgc ccgacgcgat caccggaacc
     1321 gccaccgcgt cggcgatggc gcgggtgagc gcgatatcgt agccgatctt ggtgccgtcg
     1381 cggtccatgg aggtgagcaa aatctcgccc gcgccgtatt cgaccagctt taccgcatgg
     1441 gcgacggcgt ccagccccgt cggggtgcgc ccgccatggg tgaagacctc gaacctgccg
     1501 ggcgccaccc ctttggcgtc gatggccgcg acgatacatt ggctgccgaa tttctccgct
     1561 ccttcgcgga tgaattctgg gcggcgcacc gcttcggtat tgaccgacac tttgtcggcc
     1621 ccggccagca aaagcgcgcg gatatcgtcg agtgtgcgca ctccgccgcc caccgtcagc
     1681 ggcatgaagc agacgtcggc ggtgcgccgc accacgtcga gcaggatatt gcgcttttcg
     1741 tgcgacgcgg tgatgtcgag gaagcagagt tcgtcggcgc ccatcttgtc gtagagcatc
     1801 gcctgttcga ccggatcgcc cgcgtcgcgc aggccgacga aattgatccc cttgacgacg
     1861 cggccgtcct tcacgtcgag gcaggggatg atgcggattt tcagcatggc tatgtgcccg
     1921 acggcgggtc gggaatgtcc cggaagaccg ccgccatggc cagcccgatg ccgagg
//
LOCUS       DASWPU010000132         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_68467,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000132 DASWPU010000000
VERSION     DASWPU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68467"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            491..>1814
                     /locus_tag="VGC27_02980"
     CDS             491..>1814
                     /locus_tag="VGC27_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY0281571.1"
                     /translation="MAQEILINVGAAEIRIAVVEDGKLQALTRERTLGADGEASAGRS
                     FVGDIVLGRVAKVLPAIQAAFVEIGHERAGFLGAREARCLGVLRQAQHEGSATPLTLS
                     LSKGAEEYEHPSIGALVREGEEVLVQVTKDPIGEKGARLSASVTIPGRLCVLAPFQPG
                     VALSRRIEDEAERERLSALGEALIGEAGDELFPGAGYIFRTAAVGAGLEELREDAVRL
                     ARDWRDILAARKKAKPPALLHRDLGPVARVLRDIVRDDTARILIDDAEAADAARAFCR
                     LALPEAEARIALFAGPGALFDLYDLETDIDGLLHPRVLLSCGGWITVEGTEALTAVDV
                     NSGSFTQSSGLEDTGLTVNLQAARELGRQVRLRGIGGLIVVDFIHMKEDAHVDRVLEA
                     LTQSLSWDGTPVTVSRPSALGLVEITRKRVREPLVSRLSEDCAACAGQG"
BASE COUNT          309 a          568 c          616 g          321 t
ORIGIN      
        1 gccgaaaccg cgaccctgct tcgcggctgc ggatacgtct gctgataagg caatgtgcga
       61 atgactttct gcccccgcgt cagcggggga agtgtcgccc gaagctccga aggagcgaag
      121 ggtgacgaag ggggccgcgg acgcaaattt actttgaccc cgttattact gttcttttca
      181 cagtgacgat tgcgcgagtt gatcgcgcaa agaaatctct cgcgcgctct tgaatcgcac
      241 ggacgatatt cccatatcac ttcggtcgtg ccgataaacg gagccatcga aatcgtcgaa
      301 acaattatgt tgccgaaggt gcattgagga gaatgaagtg gtgcggaggg ggataccccc
      361 ccggcaaagt cgaaaacgaa tggaggtggg tgcggcggcc aaggatcgca agatattgcg
      421 gtacgggcac attttctgat gcaatgccct gcggctcgtt ctaagtttct cctccaatcc
      481 tcaaggtccg atggcgcaag agattctcat caatgtgggc gcggcggaaa tccgcatcgc
      541 cgtggtcgaa gacggcaagc tccaggcgct gacccgcgag cgcacgctcg gcgccgacgg
      601 cgaagcctcg gcggggcgca gtttcgtcgg cgacatcgtg ctcggacgcg tggcgaaggt
      661 gctgcccgcc atccaggccg cgttcgtcga gatcggccac gagcgcgcgg gtttcctggg
      721 cgcgcgggag gcgcgctgtc ttggcgtgct tcgacaggct cagcatgagg gttccgctac
      781 ccccctcacc ctgagcttgt cgaagggtgc cgaagaatat gagcacccct ccatcggcgc
      841 gctggtgcgc gagggtgaag aggttctggt ccaggtcacc aaggacccga tcggcgagaa
      901 aggcgcacgc ctgtccgctt cggtgacgat ccccggacgg ctgtgcgtgc tggcgccgtt
      961 ccagccgggc gtcgctctct cacggcgcat cgaggatgaa gccgagcgcg agcgtctctc
     1021 tgcgctgggc gaagcgctga tcggcgaggc cggcgacgaa ctctttcccg gcgccggtta
     1081 catcttccgc accgccgccg tcggcgccgg gctggaggag ctgcgcgaag atgcggtccg
     1141 gctggcgcgg gactggcggg atatcctcgc cgcgcggaag aaggccaagc cgcccgcgct
     1201 gctgcatcgc gatctcggcc ccgtcgcgcg cgtgctgcgc gatatcgtcc gcgacgacac
     1261 ggcgcgtatc ctcatcgacg atgccgaagc ggcggacgcc gcgcgcgcct tctgccgcct
     1321 ggcgttgccc gaggccgaag cgcgcatcgc gcttttcgcg gggccgggcg cgctgttcga
     1381 tctctacgat ctcgaaaccg acatcgacgg gctgctgcat ccgcgcgtgc tgctctcctg
     1441 cggcggctgg atcactgtgg aaggcaccga ggcgctgacc gcggtggacg tcaattccgg
     1501 cagcttcacc cagtcctccg gtctggaaga cacggggctc accgtcaatc ttcaggctgc
     1561 gcgcgaactc ggacggcagg tacgcctgcg cggcatcggc ggactgatcg tcgtcgattt
     1621 cattcacatg aaggaagacg cgcatgtcga ccgggttctc gaagctctga cgcaaagcct
     1681 gtcgtgggat ggaacgccgg tgacggtttc gcgcccgtcg gcgctcggct tggtcgagat
     1741 cacccgcaag cgcgtgcgcg aaccgctcgt ctcccgcctg agcgaagact gcgcggcgtg
     1801 cgcggggcag ggga
//
LOCUS       DASWPU010000133        10901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_7138, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000133 DASWPU010000000
VERSION     DASWPU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7138"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VGC27_02985"
     CDS             complement(<1..419)
                     /locus_tag="VGC27_02985"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY0281572.1"
                     /translation="MGALLPLDTFSRRRFVNTGAASFLFPAALARAQTVPPEDAAKIA
                     AGQDTAKHLTIKVMINGKGPFDFVVDTGADRSVLAEDVAGALGLMRQKPVMVEGVVRT
                     ITAQTVHLDKLSFGPLSRDNLDVPLLPRSLLGSDGYLG"
     gene            complement(639..3425)
                     /locus_tag="VGC27_02990"
     CDS             complement(639..3425)
                     /locus_tag="VGC27_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY0281573.1"
                     /translation="MTVLTDSVRSISLWGAGRFGRRYGAAIALAGALALGACSSLPQV
                     DIGKVTSVFGGAGEPAAPAPAPEQSAAVDFGTSTEIHPGIWPVVQSGAARDPDIEAQA
                     ARLLSRMTAEEKVGQLIQADLNSVTPEDMRRYHLGSILAGGDTGPGGKLKVPAPEWLK
                     AADAYYDASVAVAPGRPVIPEIWGIDAVHGHAHIIGATLFPQNIGLGAARNPDLVRKI
                     GEITAREVRITGLDWTFAPTIAVARDDRWGRTYESYSEDPKIVSEYAAAAIEGLQGKP
                     GDTDFLMGGHIIANAKHFMADGGTERGQDQGDSIYSEEALRDIFAPPYEAAIKAGVQA
                     VMASHSSWRGKTMHGDKELLTDVLAGRFGFDGIRVGDWNGHAKVPGCTKVDCPAALNG
                     GLDMFMISSDWKALYANLLAEVKSGVIPQARLDDAVTRILRVKLRAGVMTEGRPSSRP
                     YAGRWDLFGSPEHRAVARRAVRESLVLLKNDGGILPLTPRLHVLVAGDGADNMPKQAG
                     GWTVSWQGDGTTRADFPNAETIFEGIKAQVEAAGGTAALNADGRFVEKPDVAIVVFGE
                     DPYAEWRGDRPNVDFQAKDRRDLRLLQSLRAQGIPVVSVFLSGRPLYVTPEINASNAF
                     VATWLPGSEGAGIADVLFAKPDGAVAYDFRGRLSFSWPRSPAQTTVNVGTEPYFPLFS
                     YGYGLTYGTPHNMGMLPEAVPADFPAGSPGALVESGRAAPPWSLVLSDTTGERVATDP
                     APASVPTAALRVDRGDRNKQEDVLVATWSGIGKASLVVAADQPANFSDQTDSMALTMN
                     LRVDTAPAGAVTLAMGSASRLGRVDLTRALKAAHGKGWTTIAVPLSCFRKAGADMGAV
                     TTPMTLTTSRRLTVSLFSTLVMPAAGQAVCPAAGASTVEVEARPRVKAASQVKAKPRG
                     KAKAHGKPRKGHAR"
     gene            complement(3479..4345)
                     /locus_tag="VGC27_02995"
     CDS             complement(3479..4345)
                     /locus_tag="VGC27_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEY0281574.1"
                     /translation="MITLVQGSLELDIAPETGGAVTRFAREGKDILRPAPKGAFTPLD
                     AGSFALVPYANRVENCELHFQGRKYKLRRNFGDHPHALHGHGWQTAWRVVSLSRDQAT
                     LAFDYEADDWPWTYSAEQVLTLTDGGLTHRLTLRSRDVKPMPASLGFHPYFPKTASTR
                     LTASVAGMWKSDETMIPVEHVAGSPLLDLAGGAPVDKAPFVDNCFTGWRSPARIEQPD
                     SGLTIALEASPECTFFHVFVPVGQTFFAAEPVSAMPNAFNRPEASSVTGARVLASGAS
                     FSIEMRLGVGRR"
     gene            complement(4367..5845)
                     /locus_tag="VGC27_03000"
     CDS             complement(4367..5845)
                     /locus_tag="VGC27_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEY0281575.1"
                     /translation="MAKTNYPLIAGLTISATLGGLLFGYDTAVISGATEAITHNFVAP
                     RGLPEGAANFLSGLAISCALLGCVIGAGLAGPISTSVGRRGGLIIAGILFFVSSLGAG
                     FPEFFWSLLGFDGANALPIFIFYRVMGGTAIGMASMLAPMYIAEVAPPAIRGMLVTFQ
                     QIAIVLGINLVYFVNWYIQSIGDHGFLMDLGWRYMLASAAIPSVLLIGFMLMVPETPR
                     FLVLKDKNSQALALLKRLVGADKADSTLKEIKDSLIEHTRPLLSYGWLVLIVGILLSV
                     FQQVVGINAVLYYAPRMFENMGASTNEAFWYSAVFVGVTMTLFTLVATVTVDRLGRKP
                     LLITGAVVMAAAMLVIGSLFNAHLVSATGVDGASSSTSSMVAVGAVVVYIMGFSFSWG
                     PVTWVMLSEIFPNSIKGKAMSIAVAAQWVTNFIVSQTFPMMDGSSWLNAMFNHGFSYW
                     LYSAASLIAAVFVWKYVPETKGRTLEAIEQFWHRKTAETRGA"
     gene            complement(6052..7830)
                     /locus_tag="VGC27_03005"
     CDS             complement(6052..7830)
                     /locus_tag="VGC27_03005"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEY0281576.1"
                     /translation="MASDKPKKRFRSRDWFDDPARMDMVALYFERYMNSGMTPEELRS
                     GRPIIGIAQSGSDLNPCNTVHIELAKRVREGVRDAGGVAMEFPTHPLFENCRRPTAAI
                     DRNLAYLGLVEILHGYPIDAVVLTTGCDKTTPSAIMAASTVDIPAIVLSGGPMLDGWF
                     EGELVGSGMAIWKSRRRLGAGEITEEEFVQIAAASAPSLGHCNTMGTASTMNAVAEIL
                     GLSLPGCSAIPAPYRERGQMAYATGKRIVEMAYEDLRPSNILTRQAFLNAIAAVAAIG
                     GSTNAQPHINAMARHAGVEIAPQDWMAGRDIPLLVNMQPAGKYLGERFHRAGGVPAVL
                     CELLRAGRIDGKVLTVTGKSMAENLKGRESIDREMITAYETPLQDHAGFMVMSGNLFD
                     FAIMKTSVISAEFRRRYLSEPGREGVYEGRAVVFDGAGDYHARINDPALGIDDRTILV
                     MRGAGPIGWPGSAEVVNMQPPDALLKKGIASLPTIGDGRQSGTSDSPSILNASPESAA
                     GGGLSWLRTGDTVRIDVEHGCCDALVSEEEIARRKQEAPPPIPASQTPWQEIFRATTG
                     QLDKGAVMELAVKYRGVAAKTPRHNH"
     gene            complement(7876..8964)
                     /locus_tag="VGC27_03010"
     CDS             complement(7876..8964)
                     /locus_tag="VGC27_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0281577.1"
                     /translation="MSSTKNLPNTPQKNGRKHRGVATIHDVAQRAGVSPMTVSRVVNS
                     EKNVREATRAAVMTAVHDLNYAPNPAARSLAGASAARIGLLYSNPSAAYLSEFLVGAL
                     DETSRKSAQIILEKCELSSAAAERAAVRKLIDGGAAGAILPPPLCESPDVLDELKAAE
                     LPAVAVATGCFNAQASCVRINDYAAAYEMTAYLISLGHRHIGFIKGHPNQTASVERER
                     GYEAALREAGVRSDKTLIVQGYFSYRSGLEAAENLLTRKAAPTAIFASNDDMAAATVS
                     VAHRKGLDVPGDLSVVGFDDTAIASTISPELTTIRQPITTMAEVAVDLVMRAVRERRA
                     GREPKLVDHLVTYSLVKRQSAGILKVSR"
     gene            9491..10426
                     /locus_tag="VGC27_03015"
     CDS             9491..10426
                     /locus_tag="VGC27_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0281578.1"
                     /translation="MFRVGLIGIGKVARDQHIPVLSANPHFELVACTSRNNRVETVAN
                     FSTVEEMLAACPQLDAVVVCSPPQVHYEAAKRALLAGKHVFMEKPPCATTAELGRLTQ
                     LARIHKRTLFQTWHLRYAPRVDDAKRWLKERKVLGVRIVWKEDVRQWHPGQSWIFQAG
                     GFGVFDPGINALSVLTKILPEPVFVESAEHFVPENCQAPIAADLVLRTDSGARISAAF
                     DFLYSGDPIWDIEVQTDTQTLKLSLAATVLSIGGRELEFPPHEDGIHAEYVPLYRRFA
                     ELIAAGESEIDSTPFRLVADAFLAGKRTQVARFEE"
     gene            complement(10438..>10901)
                     /locus_tag="VGC27_03020"
     CDS             complement(10438..>10901)
                     /locus_tag="VGC27_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0281579.1"
                     /translation="RLTLHTDFALRLLTLLALEPDGLHTIAEAARRYNISRNHLVKVA
                     QTLIQAGFVESVRGRSGGLKLAMRPAEIVLGAVVRATEDNFDLVECFDRKRNCCVVMP
                     ACGLRRPLEQAVSAFLSALDGYTLADAIQNSGRMTRMRRLLDDGASLSSRL"
BASE COUNT         1901 a         3552 c         3521 g         1927 t
ORIGIN      
        1 ccgagatagc cgtcgctgcc caacagcgag cggggcaaga gaggcacatc gagattgtcg
       61 cgggaaagcg ggccgaacga gagcttgtcg aggtgaacgg tctgggcggt aatcgtgcgc
      121 accacgcctt ccaccataac cggcttctgg cgcataagcc cgagggcgcc cgcgacatcc
      181 tcggcgagga cgctgcgatc ggcgccggta tcgacgacga aatcgaacgg ccccttgccg
      241 ttgatcatga ccttgatcgt gaggtgtttg gcggtgtcct gccccgccgc gattttcgcg
      301 gcatcctccg gcgggacggt ttgggctctt gcgagcgccg ccggaaacaa aaacgaggcg
      361 gcgcccgtat tcacaaatcg ccggcgagaa aacgtatcga gcggtaacaa ggcacccatc
      421 tcagcctccc gcacacccgt ctgaagggtg tttattatta tttacaatat aaccgttgag
      481 cgcccttgtg tcgaaaatat gtcgaagact ttggtaatct tctaattcac tgcaaaacac
      541 ccccaaaaat cgcggcttgg tatggaggac ggtttcaaag ctttgccttg accgccgcta
      601 gcctgaggct gtgcgccttg cgcctggtct aaacgctctt acctggcgtg ccctttccgc
      661 ggcttgccgt gcgcttttgc tttgccgcgc ggctttgcct tcacctgtga cgcggctttg
      721 acgcgcggcc ttgcttcgac ctccaccgtc gaagcccctg ccgcggggca gaccgcttga
      781 cctgcggcgg gcataacgag ggtcgagaaa aggctgaccg tgagcctgcg gctggtggtc
      841 agcgtcatcg gcgtcgtcac cgcgcccatg tccgcgccgg ccttgcggaa acacgacaac
      901 ggcaccgcga tcgtcgtcca gcccttgccg tgtgccgcct tgagcgcccg tgtcagatcg
      961 accctgccca gacggctcgc gctgcccatg gcgagcgtca ccgctccggc tggcgcggta
     1021 tcgacgcgca ggttcatggt cagagccata ctgtcggtct ggtccgaaaa attcgccggc
     1081 tggtccgccg ccacgacaag actggctttg ccgatgccgc tccaggtcgc gacgagcacg
     1141 tcttcctgtt tgttgcggtc gccgcgatcg actcgcagcg cggcggtggg caccgatgcg
     1201 ggcgccggat cggtggcgac cctttcgccg gtcgtatcgc tcagcacgag cgaccagggc
     1261 ggcgcggcgc ggcccgattc caccagcgca cccggcgagc ccgcggggaa atcggcgggc
     1321 accgcctcgg gcagcattcc catgttgtgc ggcgtgccgt aggtcagacc gtagccgtag
     1381 gagaacagcg gaaaataggg ttccgtgccg acattgaccg tcgtctgggc tggcgaacgc
     1441 ggccaggaaa aggagagcct gccgcggaaa tcgtacgcca ccgcgccgtc gggtttggcg
     1501 aacagcacat cggcaatgcc cgcgccttcc gaaccgggca gccaggtcgc gacaaaggcg
     1561 ttggaggcgt tgatctcggg tgtgacatag agcggccgcc ccgacaggaa tacgctcacc
     1621 accggaatgc cctgtgcgcg caggctttgc agcagtctca gatcgcgtct gtccttggcc
     1681 tggaaatcga cgttcggccg gtcgccgcgc cactccgcat acggatcctc gccgaagacc
     1741 acgatggcga cgtccggctt ctcgacgaaa cggccgtcgg cattgagcgc ggcggttccg
     1801 cccgccgcct ccacctgggc cttgatgcct tcgaagatcg tctcggcgtt gggaaaatcg
     1861 gcgcgcgtcg tgccgtcgcc ctgccaggag acggtccagc cgcccgcctg cttgggcata
     1921 ttgtccgcgc cgtcgccggc caccagcaca tgcagccgcg gcgtcagcgg caggatgccg
     1981 ccgtcgtttt tcagcagcac cagcgattcg cgcaccgccc ggcgcgccac cgcgcgatgt
     2041 tccggcgaac cgaacaaatc ccaccgcccc gcatagggcc gcgacgaagg cctgccttcg
     2101 gtcatcacgc cggcgcgaag cttcacgcgc agaatgcgcg tcaccgcgtc gtccagccgg
     2161 gcttgcggga tgacgccgga tttgacctct gccagcaggt tcgcatagag cgctttccag
     2221 tcgctcgaga tcatgaacat gtcgaggccg ccgttgagcg cggccggaca atcgaccttg
     2281 gtgcagcccg gcaccttggc gtgcccgttc caatcgccga cgcggatgcc gtcgaagccg
     2341 aagcgtccgg ccaggacgtc ggtcagcaac tccttgtcgc cgtgcatcgt cttgccgcgc
     2401 cagctcgaat gggacgccat caccgcctgc acgcccgcct tgatcgcggc ttcgtagggc
     2461 ggcgcgaaaa tatcgcgcag cgcctcttcg ctgtagatgc tgtctccttg gtcctggcct
     2521 ctctcggtgc cgccgtcggc catgaaatgc ttcgcgttcg cgatgatgtg gccgcccatc
     2581 agaaaatccg tatcgcccgg cttgccttgg agaccctcta tggccgccgc cgcatattcg
     2641 ctgacgatct tcggatcctc cgaatagctt tcgtaggtac gcccccagcg atcgtcgcgc
     2701 gccacggcga tggtgggggc gaaggtccag tccagtccgg ttatgcggac ttcccgcgcg
     2761 gtgatctctc cgatcttgcg caccagatcg ggattgcgcg cggcgccgag tccgatgttc
     2821 tgcgggaaca gcgtagcgcc gatgatatgg gcgtggccgt gaacggcgtc gatgccccag
     2881 atttccggaa tcacgggccg tccgggcgcg acggccacag aggcatcgta atacgcgtcg
     2941 gccgccttca gccattcggg cgccggcact ttgagtttgc cgcccgggcc cgtgtcgccg
     3001 ccggcgagga tggagccgag atggtaccgg cgcatgtctt cgggcgtcac cgagttgagg
     3061 tcggcctgga tgagctggcc taccttctct tccgccgtca ttcgcgaaag cagccgcgcc
     3121 gcttgcgcct cgatgtcggg atcgcgcgcc gcaccgcttt ggaccaccgg ccaaatgccg
     3181 ggatgaatct cggtcgaggt tccaaaatcg acagccgcgg attgctccgg cgccggcgcc
     3241 ggagcggccg gctcgccggc gccgccgaaa acggaagtga ctttgccgat gtccacctgc
     3301 ggcaaggacg agcaggcgcc gagcgccaag gcgcccgcca acgcgatggc cgccccgtac
     3361 cgccggccga agcggcccgc accccaaagc gaaatactcc gtacactgtc agtcagcacc
     3421 gtcatgtttc ccgccttgag tggagagagg ccgaaaggct gatgggattt atgcactatt
     3481 acctgcggcc gacgccgagg cgcatctcga tcgagaagct tgcgccggaa gccagcacgc
     3541 gcgcgcccgt tacgctcgaa gcttcggggc ggttgaaggc gttgggcatg gcgctcaccg
     3601 gctcggccgc gaagaaggtc tgtccgaccg gcacaaagac gtgaaaaaag gtgcattcgg
     3661 gcgacgcttc cagcgcgatc gtcaagccgc tgtccggctg ctcgatccgc gccggggagc
     3721 gccagccggt aaaacaattg tcaacgaacg gcgccttgtc caccggtgcg ccgcccgcca
     3781 gatcgagcag cggcgagccg gccacatgct ccaccggaat catggtctcg tcgcttttcc
     3841 acatccccgc caccgaagcg gtcaaccttg tggaagccgt cttgggaaaa taggggtgga
     3901 agcccaggct ggcgggcatc ggcttgacgt cgcggctgcg cagcgtgagg cggtgggtta
     3961 atccgccgtc ggttagggtg agaacctgtt cggcgctgta ggtccagggc caatcgtccg
     4021 cttcgtaatc gaaggccagc gtcgcctggt cgcgcgacag cgaaaccacg cgccaggcgg
     4081 tctgccagcc atggccgtgc agggcatggg gatggtcgcc gaaattccgg cgcagcttgt
     4141 atttgcggcc ctggaaatga agctcgcaat tctcgacgcg gttggcgtag ggcaccagcg
     4201 cgaagctgcc ggcgtcgagc ggggtgaagg cgcctttcgg cgccggacga aggatgtcct
     4261 tgccctcgcg ggcgaagcgg gtcaccgctc cgcccgtttc gggcgcgata tcgagttcga
     4321 ggctaccctg taccagcgtg atcatgatac cgccgttccc gttgcgttat gcacccctcg
     4381 tctcggcagt cttgcgatgc cagaactgct cgatggcttc gagcgtgcgg cccttggttt
     4441 ccggcacgta tttccatacg aacacggcgg cgatcaggct tgcggcgctg tagagccagt
     4501 acgagaagcc gtggttgaac atggcgttca gccaggagct gccgtccatc atcgggaagg
     4561 tctgcgaaac gatgaagttg gtgacccact gcgccgccac ggcgatggac atggccttgc
     4621 ccttgatgga gttggggaag atttccgaca gcatcaccca ggtgaccggc ccccaggaga
     4681 aggagaagcc catgatgtaa acgaccaccg caccgaccgc gaccatcgaa gaggtggagg
     4741 aagacgctcc gtcgacgccc gtggcgctga cgagatgcgc gttgaacagc gacccgatca
     4801 ccagcatcgc cgccgccatg accaccgcac cggtgataag cagcggcttg cggcccagcc
     4861 ggtcgacggt gacggtggcg accagcgtga acagcgtcat cgtcacgccg acgaacacgg
     4921 ccgaatacca gaaggcctcg ttggtggagg cgcccatgtt ctcgaacatc ctcggcgcat
     4981 aatagagcac cgcattgatg ccgacgacct gctggaacac cgacagaaga atgccgacga
     5041 tcagcaccag ccagccgtag ctcagcagcg gacgggtgtg ttcgatcagc gagtccttga
     5101 tttccttcag cgtgctgtcg gccttgtccg cgccgacgag acgcttcaac agcgccagcg
     5161 cttggctgtt cttgtccttc agcaccagga agcgcggcgt ttccgggacc atcagcatga
     5221 agccgatgag cagcacggac gggatcgccg ccgaggccag catatagcgc cagccgagat
     5281 ccatcaggaa gccgtggtcg ccgatgctct ggatgtacca gttgacgaaa tagaccaggt
     5341 tgatgccgag cacgatggcg atctgctgga aggtgaccag catgccgcgg atcgccggcg
     5401 gcgccacttc ggcgatgtac atcggggcca gcatcgaggc catgccgatg gccgtgccgc
     5461 ccatgacgcg atagaagatg aagatgggaa gcgcattggc gccgtcgaag cccaacaggc
     5521 tccagaagaa ctccggaaag cccgcgccga gcgaagacac gaagaacaat atgccggcga
     5581 tgatcaaccc gcccctgcgc ccgaccgagg tcgagatcgg tcccgcaagg cccgcgccga
     5641 tgacgcagcc gagcagcgcg caactgatcg ccaagcccga aaggaagttc gcggcgccct
     5701 ccggcaaacc gcgcggcgcg acgaagttat gcgtgatggc ttccgtcgcg ccggaaatga
     5761 cggcagtgtc gtagccgaac agcaaacctc ccaaggtcgc gctgatcgta agacctgcaa
     5821 ttaacggata attggtcttg gccattgagg acccctctat aaacgcagcg ttccccttct
     5881 cccttaccgg gagcgagatt tacaacctca ctaggcttgt taccctaccc tgcccggcgg
     5941 cgtgaccgta gtggtagcgc taacgctctg caagcacggt tcgttcaact aagcctggta
     6001 ttttccgaca ttaatagcgc tgtccgttcg caaatccatg ggaatcacgc atcagtgatt
     6061 gtgccgcgga gtcttggcgg cgacgccgcg atatttcacc gcaagttcca tcaccgcgcc
     6121 cttgtcgagc tgcccggtcg tggcccggaa tatctcttgc catggcgtct ggctggcggg
     6181 gatcggcggc ggcgcttcct gcttgcgccg ggcgatctcc tcctcggaca ccaatgcgtc
     6241 gcaacagccg tgctccacgt cgatgcggac cgtatcgccg gtgcgcagcc aggacagccc
     6301 gccgcccgcc gcgctttccg gcgaggcgtt gaggatggag gggctgtccg aagtgcccga
     6361 ctgccgcccg tcgccgatgg tcggcaagct ggcgatgcct tttttcagca gcgcatccgg
     6421 cggttgcatg ttgacgactt ccgccgagcc cggccagccg atgggacccg cgccgcgcat
     6481 caccaggatg gtgcggtcgt cgatgccgag cgccggatcg ttgatgcggg cgtgataatc
     6541 gccggcgccg tcgaacacca ccgcgcgccc ttcatagacg ccttcgcggc ccggctcgct
     6601 cagatagcgg cggcggaatt ccgcggaaat cacgctcgtc ttcatgatcg cgaaatcgaa
     6661 caggttgccg ctcatcacca tgaatcccgc atggtcctgg agcggcgtct cataggccgt
     6721 gatcatttcg cggtcgatgc tttcccggcc tttcaggttc tcggccatgc tcttgccggt
     6781 gacggtgagc accttgccgt cgatccttcc ggcgcgcagc agttcgcaca gcacggccgg
     6841 cacgccgccg gcgcgatgga agcgctcgcc cagatatttc cccgccggct gcatattgac
     6901 gagcaaggga atgtcgcggc ccgccatcca atcctgcggc gcgatctcga cgccggcatg
     6961 gcgcgccatg gcgttgatgt gcggctgggc gttggtcgag ccgccgatgg cggcgacggc
     7021 ggcgatggcg ttgagaaacg cctgccgcgt caggatgttc gacggccgca gatcctcata
     7081 ggccatttcg acgatgcgct tgccggtggc gtaggccatc tggccgcgct cgcgataggg
     7141 cgcggggatc gccgaacagc cgggcagcga aagccccaga atttccgcga cggcgttcat
     7201 ggtggaagcc gtccccatcg tgttgcaatg gccgagcgac ggcgccgagg ccgcggcgat
     7261 ctgcacgaac tcttcctcgg tgatctcgcc cgcgccgagg cgccggcggc tcttccagat
     7321 cgccatgccg gagcccacca gctcgccctc gaaccagccg tccagcatcg gtccgcccga
     7381 cagcacgatc gcgggaatat cgaccgtgga cgccgccatg atcgccgacg gcgtggtctt
     7441 gtcgcagccc gtggtcagca ccaccgcgtc gatgggatag ccgtgcagaa tctccaccag
     7501 ccccagatag gcgaggttgc ggtcgatggc ggcggtgggc cgccggcaat tctcgaacag
     7561 cggatgggtg ggaaattcca tcgccacgcc gccggcgtcg cggacgcctt cgcgcacgcg
     7621 cttggccagt tcgatatgca ccgtgttgca ggggttgagg tcgctgccgc tctgcgcgat
     7681 gccgatgatc gggcggcccg aacgcaattc ctccggcgtc attccgctgt tcatgtagcg
     7741 ttcgaaatag agcgccacca tgtccatgcg cgcgggatcg tcgaaccagt cgcgcgaacg
     7801 gaaccgtttc ttcggtttgt cagacgccat cgctcactcc tgccgccgcc gcccgcgtcg
     7861 agaccggggc aatcgttacc ggctcacctt cagaattccc gctgattgcc gcttgaccag
     7921 agaatacgtg accagatgat cgacgagttt gggttcgcgt cccgccctgc gctcgcggac
     7981 cgcacgcatg acgagatcga cggcgacttc ggccatggtc gtgatcggct gacggatggt
     8041 cgtcagctcg ggcgagatgg tagaggcgat cgcggtatcg tcgaagccca ccaccgaaag
     8101 atcgcccggc acgtcgaggc ccttgcggtg cgccaccgag accgtagcgg cggccatgtc
     8161 gtcgttgctg gcgaatatcg cggtgggagc ggctttgcgc gtcaacaggt tctccgccgc
     8221 ctccaggccc gaacggtagc tgaagtaacc ctgcacgatc agggttttgt cgctgcgcac
     8281 gccggcttcg cgcagggccg cttcgtaacc gcgttcgcgc tccacgctgg cggtctggtt
     8341 ggggtggccc ttgatgaagc cgatatggcg atggccgagc gatatgagat acgccgtcat
     8401 ctcgtaggcg gcggcataat cgttgatgcg cacgcaggag gcctgggcgt tgaaacaccc
     8461 ggtcgcgacc gccaccgccg gcagttccgc ggccttgagt tcgtccagca cgtccggcga
     8521 ttcgcacaag ggcggcggca ggatcgcacc ggcagcgccg ccgtcgatca gcttgcgcac
     8581 ggcggcgcgc tccgccgcgg cgctggagag ttcgcacttc tccagaatga tctgggcgct
     8641 cttgcggctg gtttcgtcga gggcaccgac cagaaattcg ctcagatagg cggcgctcgg
     8701 gttgctgtag agcaggccga tgcgcgcggc cgaggcgccg gccaagctgc gcgcggcggg
     8761 atttggcgcg taattgagat cgtggaccgc ggtcatcacc gcggcgcgcg tcgcttcgcg
     8821 cacgttcttc tcgctgttga cgacgcgcga gaccgtcatc ggcgaaacgc cggcgcgctg
     8881 cgccacgtcg tgtatcgtcg cgacgccgcg atgctttctg ccattcttct gcggggtgtt
     8941 cggcaagttc ttggtgctgc tcatgcaagg cgggtaaagc acagcttacc gcatcggctg
     9001 cgcctcttcc aactgccgtt ttggcgcgcc ctcgtccggc tcgacgatgc cgccgcgcag
     9061 cggcggactg ccggcgcctt ggccgcagga aaaagaaatg cgtgctgcgg cgagcgcgaa
     9121 ccctgccgcc gccggtgtcg cggcgcggcc gggggccgcg aggacgccct ctcatgcatg
     9181 aaggcgaacg ccgataaaac acctcccctt cttgtttgcg aacgcctata ttccgcaccc
     9241 ggccgggcgt gccggatgaa gcaccgcgaa tgcgcgggcg gttgccggct ttgggtttgc
     9301 ggcaggggag tcagaggtat tctaaagtat cgcacggaac gatggcttag gtgcctggga
     9361 caggtgtttg gaggtgaccc ttcacttata atataaataa tattgtggag aagtccagcg
     9421 gcggacgcct tccccgctac cgctcgctgc agcagccata gccgatctgt tgaggagaat
     9481 gccggtggcc atgtttcgcg tgggtttgat cggaatcgga aaggtggcgc gcgatcagca
     9541 catcccggtg ctgagcgcga acccgcattt cgaactcgtc gcctgcacga gccgcaacaa
     9601 ccgcgtcgaa accgtggcga atttctcgac cgtcgaggag atgcttgcgg cttgtccgca
     9661 gttggacgcg gtggtggtct gctcgccgcc gcaggtgcat tacgaagcgg ccaagcgcgc
     9721 gctgctggcc ggcaaacatg tgttcatgga gaagccgcct tgcgccacca cggcagagct
     9781 tgggcgattg acgcagttgg cgcggattca caagcggaca ttattccaga cctggcacct
     9841 gcgctacgcg ccgcgcgtcg acgatgcgaa acgctggctc aaggaacgca aggtcctcgg
     9901 cgtgcgcatc gtctggaagg aagacgtacg ccagtggcat ccgggacaaa gctggatttt
     9961 ccaggccggc ggcttcggtg tcttcgatcc cggcatcaac gccctttccg tcctgaccaa
    10021 gatcctgccc gaaccggtgt tcgtggagtc ggccgagcat ttcgtgcccg agaattgcca
    10081 agcgccgatc gccgccgatc tggttctgcg caccgatagc ggcgccagga tctcggccgc
    10141 cttcgatttt ctctacagcg gcgacccgat ctgggacatc gaggtgcaga ccgacacgca
    10201 gacgctgaag ctgtcgctcg ccgccaccgt gctttcgatc ggcgggcggg agctggaatt
    10261 cccgccgcac gaagacggca tccacgccga atatgtgccc ctctatcgcc gtttcgccga
    10321 actcatcgcc gccggagaat cggagatcga ctcgacgccg ttccgcctcg tcgccgacgc
    10381 cttcctggcg ggcaagcgca cgcaagtggc gcgtttcgag gaatagaacc gtccggatta
    10441 aagccgggac gaaagcgacg cgccgtcgtc gagcagacgg cgcatgcgcg tcatccggcc
    10501 ggagttctgt atcgcatccg cgagggtgta gccgtcgagg gcggagagaa aggcggatac
    10561 ggcctgttcg agcggccggc gcaggccgca ggccggcatc accacgcagc agttccgctt
    10621 gcggtcgaag cattcgacca ggtcgaaatt gtcctcggtg gcgcggacca ccgcgcccag
    10681 aacgatctcc gccggtctca tggcgagctt gagcccgccg ctgcggccgc gcacgctttc
    10741 gacgaagccc gcctggatca gcgtctgcgc caccttcacc agatgattgc gcgagatgtt
    10801 atagcgccgc gccgcttccg cgatggtgtg cagtccatcg ggttccagcg ccagcagcgt
    10861 caacagccgc agggcaaagt ccgtatgcag agtaagccgc a
//
LOCUS       DASWPU010000134         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_68468,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000134 DASWPU010000000
VERSION     DASWPU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68468"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..480)
                     /locus_tag="VGC27_03025"
                     /pseudo
     CDS             complement(247..480)
                     /locus_tag="VGC27_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002832300.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
     gene            complement(849..2291)
                     /locus_tag="VGC27_03030"
     CDS             complement(849..2291)
                     /locus_tag="VGC27_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0281580.1"
                     /translation="MGATFSLFRLPARCGSALLLAAPLWFAASPAAADWYGDQPSDPR
                     ASETAALLDPFDPVPEIGRGDCRDGWYGCRRFDRFRDYDGRGRGQWDHGRWGHGRGPL
                     HLSDRGALMVDCSGQHRNMFSSVGAAAHHAPPNATILILPPGDGGTCVETVAIDRPLT
                     IATLGGGSPAVIQAPANQPCLIADIPLGDTLTLDRIRFIARGGKAPCVSVQAGHVVVR
                     NSSIDSRTRDWAFDVAESGALNLEATRIETDGSGVHARRAKVELHDFAITIDSGHRGV
                     ALALDRTDGTVDGGSIVGGSTGILASAGTRGLQLNDTKVHKAGTGVALLPGGLGSIAA
                     DRLDLSSNADGLVIGPGAESRITGSVITNSGESGIAIYGAGTLVSGNKIVGARTGIRL
                     SPPGMFSPPHAAEFMAEPPAGGHDGRPIVENNLVANVRYAAVAADEHAHGRVMGNVFY
                     APGRAHCIAGGRYIEDRANACHGSWLPWPF"
     gene            complement(2294..>3099)
                     /locus_tag="VGC27_03035"
     CDS             complement(2294..>3099)
                     /locus_tag="VGC27_03035"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0281581.1"
                     /translation="GGGYYGDSEPSAIVRSNADALMYFDIAANLGHPLAKDYVGAHQA
                     AMSQYGDIPAITADAAARARNWAPPFEYYPGATAGGVPHSDEGLPNLVQRRALARVPE
                     IPFPAFEKALTFRGLLRGRQGPWRAASPADVAQAVRKFQSALNFEPSGFLRPHEAVRL
                     IRMSAVDGDASSQNRLGIMYAKGIGVPQNFPRAEKWFFLAARQHHGEAIYNLGVLYKV
                     GPNGVEQDKDKAARLFAESALAGFNPTNCELRELLSQAADTDRNRGARR"
BASE COUNT          526 a         1007 c         1030 g          536 t
ORIGIN      
        1 gtcgctgccg ccatcgtcgg gcatgatgaa gccgaaacct ttctggtcat tgaagaactt
       61 caccttgcct tcgaccatgg gtccgctcgg cccggaattc tggtcgcgag agccgttgcc
      121 gccgaaacgg tcgccgccgc tgaagcgatc gtcaccgcca aagccgccgc tacgcccgcc
      181 ttcgccgaat tctcggcgtg gacggcgcgg cggactgggg tctgccgtgc tggtgtccac
      241 gttatgaacg gtgacgacct gcagcccgcg cggagattcc tgcagatcgc aaacgatggt
      301 tgcgccttgc ggaagattgg aggtccctag cgccgccagc gccgaggcat gacaaaaagc
      361 gtcgctaccg ctctccagcg tgacaaaacc atatcctttg ctcgcgttga accatttgac
      421 cgtgcccttc acagaagcct gcgcgagggg ctgcgagccg tcattgtcga agtttgccat
      481 gagtatgccg atgctacctg ccggacatga tcccacgcgc gcaccagctc atccggccgc
      541 ggcacagtgc gtcaggatga catcatgcct aaaaacaaag cgaatccgca agccgtccgc
      601 ctggcctcgc cggggtctgg tccggaaggg gcggattcct ggaatttccg gtgtgtcaca
      661 agggcggcag tccgcgcttt ttcgcgcctg cggaaaactt tttctgacaa tgagtattcg
      721 tattgttcag caactatccg cccgcgctag agcatttcac cgtttcacgg aaccgatgaa
      781 atgctctaga ttcttagttt gtcgcgtttt cggacggaaa accggtttcc acttttcctg
      841 aaaacgcgct agaacggcca gggtagccaa gacccgtgac aggcgttggc gcggtcctcg
      901 atatagcggc cgccggcgat gcagtgcgca cggccgggcg cgtagaacac gttgcccatg
      961 acgcggccat gggcatgttc gtctgccgcc accgccgcat agcgcacatt ggcgacgaga
     1021 ttgttctcga cgatcggtct gccgtcatgg ccgccggccg gaggctccgc catgaactcg
     1081 gctgcatgcg gcggcgagaa catgccgggc ggcgaaagcc tgatgccggt tcgcgcgccg
     1141 acgatcttat tgccgctgac cagcgtgccg gcgccgtaga tcgcgattcc gctctcgccg
     1201 ctgttggtga tcaccgagcc cgtgatcctg gattcggcgc cgggaccgat gaccagcccg
     1261 tctgcgttgc tcgacagatc gaggcggtcg gcggcgatac tgcccagtcc gcccggcagc
     1321 agcgccacgc cggttccagc cttgtggacc ttggtgtcgt tcaactgcaa tccgcgagtc
     1381 ccggcggagg ccaggatgcc tgtcgatccg ccgacgatgc tgccgccgtc caccgtgccg
     1441 tcggtgcggt caagcgccag cgccaccccc ctatgcccgc tgtcgatggt gatggcgaag
     1501 tcgtgaagtt cgaccttcgc gcgccgcgca tgaacgccgg aaccgtcggt ttcgatgcgc
     1561 gtcgcctcaa ggttcagagc gccggactcg gcgacgtcga aggcccagtc gcgcgtgcgc
     1621 gagtcgatgg agctgttgcg caccacgaca tggccggctt gaacggagac gcaaggcgcc
     1681 ttgccgccgc gcgcgatgaa gcggatgcga tccagggtca gcgtgtcgcc gagcgggatg
     1741 tcggcgatca ggcaaggctg attcgcgggg gcctggatga cggcggggct gccgcccccc
     1801 aaggttgcga tcgtcagcgg ccggtcgatt gccaccgtct ccacacaggt gccgccatcg
     1861 cccggcggca ggatgaggat cgtcgcgttg ggcggcgcgt ggtgggcggc cgcccccacc
     1921 gaggagaaca tgttgcggtg ttgaccgctg caatcgacca tcagcgcgcc gcgatccgac
     1981 agatgcagcg gcccccggcc gtggccccac cggccatgat cccactggcc gcggcccctt
     2041 ccgtcatagt cgcggaaacg gtcgaaacgg cggcagccgt accatccgtc gcggcaatcg
     2101 ccgcggccga tctcgggcac cgggtcgaac ggatcgagca gcgcggcggt ttcgctggcg
     2161 cgcggatcgg acggctgatc gccgtaccaa tccgcggcgg ccggcgaggc ggcaaaccag
     2221 agcggagcgg cgaggagaag cgcgctgccg cagcgtgcgg gcaggcgaaa caaagagaag
     2281 gtcgcgccca tgattatctc cttgctccgc ggttgcgatc ggtgtcggcg gcctgcgaca
     2341 gcagctcgcg caattcgcaa ttggtgggat tgaagccggc gagcgcggat tcggcgaaca
     2401 aacgggccgc cttgtccttg tcctgttcca cgccgttggg gccgacttta tagagcacgc
     2461 cgagattgta gatggcctcg ccgtgatgct gcctggcggc aaggaagaac catttttccg
     2521 cgcgcgggaa attttgcggc acgccgatcc ccttggcgta catgatgcca agccggttct
     2581 gcgaactcgc gtcgccgtcc accgccgaca tccggatcag gcgcacggcc tcatgcgggc
     2641 gcaggaaacc ggaaggctcg aagttcagcg cgctctgaaa ctttctgacg gcctgcgcca
     2701 cgtcggcggg cgaggcggca cgccaggggc cttgccggcc tctcagcagg ccgcggaacg
     2761 tcagagcttt ctcgaaggcc gggaagggaa tctccggcac gcgcgctagc gcccggcgct
     2821 ggaccaggtt gggcaggccc tcgtcggaat ggggaacgcc gccggcggtg gcgcccggat
     2881 aatattcgaa cggcggcgcc caattgcgcg cgcgcgcggc ggcatccgcc gtaatcgccg
     2941 ggatatcgcc atactgggac atcgcggcct ggtgcgcgcc gacatagtcc ttggcgagcg
     3001 ggtggccgag attggccgca atgtcgaaat acatcagcgc atcggcgttg ctgcgcacga
     3061 ttgccgacgg ctcgctgtcg ccgtaatagc cgccgcctg
//
LOCUS       DASWPU010000135         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_49917,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000135 DASWPU010000000
VERSION     DASWPU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49917"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VGC27_03040"
     CDS             complement(<1..293)
                     /locus_tag="VGC27_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281582.1"
                     /translation="MFRTAVTAAFLVAFVGQANAAAITDWNQIAKVFDTIPSAAGISV
                     HKRMDKCGVKIVKDKSWMETRAVDWPEYGARAGETVLLITIKIPSAAGQPPSM"
     gene            complement(378..638)
                     /locus_tag="VGC27_03045"
     CDS             complement(378..638)
                     /locus_tag="VGC27_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0281583.1"
                     /translation="MSIPGWILFGLIAGFIANKIVNDRGEGCFLNIALGVVGALVGGG
                     IFALLGEPVFFRFSLWSMFVAVLGAVIVLYLWHAVTGRRRLR"
     gene            1010..1408
                     /locus_tag="VGC27_03050"
     CDS             1010..1408
                     /locus_tag="VGC27_03050"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEY0281584.1"
                     /translation="MKTVLFAATLLLAAVAAPAFAADNSCLIHRYIDGWGAHGDHAMI
                     VNDTFGRKYLLSLAGYCSDLDFSMAVGIRSPIGGGNFCVGRGDRIVMRGGGAMARNDT
                     CWVTKVERYTPEMEKAYRAQLEAKKNKPAN"
     gene            complement(1478..>1730)
                     /locus_tag="VGC27_03055"
     CDS             complement(1478..>1730)
                     /locus_tag="VGC27_03055"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY0281585.1"
                     /translation="VKTGAEGVYAGMIPERGLGIALKIDDGAARAAETTIAAILDKLG
                     LAGDGALEFLHAPVVNMRGEIVGERRPAAALSEADLAGL"
BASE COUNT          345 a          512 c          545 g          328 t
ORIGIN      
        1 tgcattgacg gcggctggcc cgcggcggag ggaatcttta tcgtgatcag aaggacggtc
       61 tcgccggccc gtgcgccata ttccggccaa tcaacggcgc gggtttccat ccaggatttg
      121 tccttcacga tcttgacgcc gcatttgtcc atgcgcttgt gcacggagat tcccgcggcg
      181 gagggaatgg tgtcgaacac tttggcgatt tggttccagt cggtgatggc ggccgcattg
      241 gcttggccaa cgaaagcgac gagaaacgcg gcggtaacgg cggtcctgaa catggcgacc
      301 ccctccggat tgcggccgga atatagcact attggcgcgc cggcggagcg aattcgcctt
      361 tctgttgcga gtcgtattca acgcaagcgg cgtcgtcccg tcacggcgtg ccacagatag
      421 agcacgatca ccgcgcccag aacggcgaca aacatcgacc acaggctgaa gcggaagaaa
      481 accggctcgc ccaggagcgc aaagataccg ccgccgacca gggcgcccac gacgcccagc
      541 gcgatgttga ggaagcagcc ttcgccgcgg tcgttgacga tcttgttggc gatgaagccg
      601 gcgatcagcc cgaacaaaat ccagccggga atggacattt cagcagcctc catgttggca
      661 ggaagatgag gggctagagg ccggtctgca agcccacctt ctcggcccta tccacctcag
      721 tggcgctatc gttgccacga tcttgctttg ggccctgcgc tccatcaaca gatggatagc
      781 ttcgctcgcg cgttcgggcg ggaagttcga cggggcattc agcatggctt tcatgaaatc
      841 tttcaaacct tgcggaaata tttctcgcta agaccgttgg cgcggtagat gccgacgccg
      901 ggatgacgga cttcgcgcct ccgaacggca tgtaggagtc gaatcggcgc ggtattgatg
      961 tataatatag gggagagggg cacccgcttc ataccgagga ccgggagcca tgaagaccgt
     1021 actttttgcc gcaacgctgc ttcttgcggc ggtggccgcg cctgctttcg cagccgataa
     1081 ttcctgcttg atacaccgct atatcgatgg atggggcgcg catggcgacc atgccatgat
     1141 cgtcaacgac acatttgggc gcaaatatct gctgagcctg gcgggctatt gctcggatct
     1201 cgacttcagc atggccgtcg gaatccgttc gccgatcggc ggcggaaatt tttgcgtcgg
     1261 acgcggcgac aggatcgtca tgcgcggcgg cggcgccatg gcgcgcaacg atacctgctg
     1321 ggtcaccaag gtcgagcgct acacgccgga gatggagaaa gcctacaggg cgcagcttga
     1381 ggccaagaag aacaaaccgg cgaactagta tcgtggtctc gaaattcgca ccatccgcca
     1441 tattgggctg caagcgaact tcgaagccaa aacgacacta gagccccgcg aggtcggcct
     1501 ctgaaagcgc cgctgccggc cggcgttcgc cgacgatttc gccgcgcata ttgacgaccg
     1561 gcgcgtgaag gaattcgagc gcgccgtcgc ccgctaggcc tagcttgtcg aggatggcgg
     1621 cgatggtggt ttcagccgcg cgcgcggcgc cgtcgtcgat cttgagcgcg atgccaagac
     1681 cgcgttcggg gatcatcccg gcatagacgc cttcagcgcc ggtcttcact
//
LOCUS       DASWPU010000136         4543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_56114,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000136 DASWPU010000000
VERSION     DASWPU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56114"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VGC27_03060"
     CDS             <1..628
                     /locus_tag="VGC27_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281586.1"
                     /translation="VEFLSIAPGKTLVVIVGEDGQVENRLIDTPIGLPASALSEASNY
                     LNAQLRGRTLDAARAQIQAELEGERAELDALTAKIVAEGLATLTTHASGEEKVLIVRG
                     ASHLLETMEAQSDLERIRTLFDEIESKNDLIRLLELAKDGDGVRIFIGSENRLFSLSG
                     SSIVAAPYTNAQGKIVGVIGVLGPTRLNYGRIIPMVDYTAKVIGRLLG"
     gene            653..1213
                     /gene="grpE"
                     /locus_tag="VGC27_03065"
     CDS             653..1213
                     /gene="grpE"
                     /locus_tag="VGC27_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY0281587.1"
                     /translation="MADDIESPSEENPPQEQTPAEQLAAAQAQLAEMRDRMLRALAET
                     ENIRRRAERERLEISQYAIAKFARDMLQIADNFGRALAACPQELREAADPQVKAVIDG
                     VEVTERQLLSTLEQYGVKIIDTTDAKFDPNLHQAIAEVPGEGKPPGTIVHVVQTGYTI
                     GERLLRPAMVTVARKENGNSGVDTKA"
     gene            1365..2174
                     /gene="dapB"
                     /locus_tag="VGC27_03070"
     CDS             1365..2174
                     /gene="dapB"
                     /locus_tag="VGC27_03070"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY0281588.1"
                     /translation="MSDVRIVIAGASGRMGRALVRVIAETKGCVLSGALEAKGHPDLG
                     RDAGALAGVGPLGVVLADDASALLAKAGAILDFTAPKASVELAGLAAAARVVHVIGTT
                     GCSPDDEAKIRDAAGRTAIIKSGNMSLGITLLAALVKRAAAALPAFDIEIVEMHHGRK
                     VDAPSGTALLLGEAAAAGRGVALSDHTVRARDGHTGPRRQDDIGFASLRGGTVVGEHE
                     VILAGPGERVALSHVAEDRAILARGAVAAALWGQGKKPGLYAMADVLGLSD"
     gene            complement(2138..3028)
                     /locus_tag="VGC27_03075"
     CDS             complement(2138..3028)
                     /locus_tag="VGC27_03075"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY0281589.1"
                     /translation="MSETAILGEDTAQWRRMGRAIRFLSEHYLDQPRLEDAAAAAGLS
                     PFHFQRAFTRYVGVSPKAFVGHLTLGHAKEELARGASVLDAALGAGLSGPSRLHDLCL
                     KIEAMTPGDYAKAGRGVSIDYGFHDCPFGTVLVLATDKGVCGLGFGDEGEEDAMLADM
                     KGRWPKAEYRENEERTAKIAARIFDPERQKESVLPLHLIGTPWQIKVWEALLAIPSGK
                     LVTYRTIAEKVCTPRACRAVGAAVGRNPISLLIPCHRVLGSGGALTGYHWGLARKRAI
                     LALEAARKDQSERPSTSAMA"
     gene            3148..3837
                     /gene="nth"
                     /locus_tag="VGC27_03080"
     CDS             3148..3837
                     /gene="nth"
                     /locus_tag="VGC27_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY0281590.1"
                     /translation="MARPFSKSDAESFFARLKAAMPEPKTELNYINPYTLLVAVVLSA
                     QATDKGVNKATEVLFKLADTPAKMLALGEAGLIGHIKTVGLYRTKAKNVIGLSKILMA
                     QYGGAVPRAREALESLPGIGRKTANVVLNVAFGEKTIAVDTHIFRVANRTGLAPGKTP
                     REVEDGLMRIVPDAYALNAHHWLILHGRYVCTARAPKCPACVLNDLCRFAEKVEPVRT
                     PAGGRAKRQGN"
     gene            complement(3794..4141)
                     /locus_tag="VGC27_03085"
     CDS             complement(3794..4141)
                     /locus_tag="VGC27_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281591.1"
                     /translation="MIRPVLAVCLCALLSACASRPAAPIPALAPSKDPARPVGLSAQD
                     VRQFFGAPAFVRQENGAEMWRYDGAGCRAIFFLYPQGDVRTVRHVETIPPGARAATDS
                     NCLAALRARPPGS"
     gene            4301..>4543
                     /locus_tag="VGC27_03090"
     CDS             4301..>4543
                     /locus_tag="VGC27_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281592.1"
                     /translation="MTRNFDVIAMGNAIVDVIAPVDDEFLLTHNIAKGMMTLIDEYRA
                     NHLYNAFSERREIAGGSAANTMAGLASLGARGMFVGK"
BASE COUNT          872 a         1485 c         1445 g          741 t
ORIGIN      
        1 tgtggaattt ctgagcatcg ctccgggcaa aaccctggtc gtcatcgtcg gtgaagacgg
       61 ccaggtggag aaccggctga tcgacacccc gatcggcctg ccggcttcgg cgctgtcgga
      121 agcctcgaac tatctgaatg cgcaactgcg cggacgcacg ctggacgcgg cccgcgcgca
      181 aatccaggcc gagctcgaag gcgaacgggc cgaactcgac gcgctgaccg ccaagatcgt
      241 cgccgaaggt ctggcgacct tgaccaccca cgccagcggc gaagagaaag tgctgatcgt
      301 gcgcggcgct tcgcacctgc tcgaaaccat ggaagcgcaa agcgatctgg agcgcatccg
      361 caccctgttc gacgagatcg aaagcaagaa cgatctgatc cgcctgctcg aactggccaa
      421 agacggggac ggcgtgcgca tcttcatcgg ctctgagaac cggctgttca gcctctccgg
      481 ctcgtcgatc gtcgccgcgc cctataccaa cgcgcagggc aagatcgtcg gcgtcatcgg
      541 ggttttgggg ccgacgcggc tcaattacgg acgcatcatt ccaatggtcg attacaccgc
      601 aaaagtgatc gggcgtttgc tcggataagc atttcaggtt taaggacaga tcatggccga
      661 cgacatcgaa tccccttccg aagaaaaccc gccgcaggag caaaccccgg cggaacaact
      721 ggccgccgcg caagcgcagc tcgccgagat gcgcgaccgc atgctgcgcg ccctggccga
      781 gaccgaaaac atccgccgcc gcgccgagcg cgagaggctg gaaatctcgc aatacgccat
      841 cgccaagttt gcacgcgaca tgctgcagat cgccgacaat ttcggccgcg cgctggccgc
      901 ctgcccgcaa gaactgcgcg aagccgccga tccgcaggtc aaggccgtga tcgacggcgt
      961 ggaagtcacc gaacgccagt tgctcagcac tctggagcag tatggggtga agatcatcga
     1021 caccacggac gccaagttcg atcccaatct gcaccaggcc attgccgaag tccccggcga
     1081 gggcaagccg ccgggcacca tcgtgcatgt ggtgcagacc ggctacacca tcggcgaaag
     1141 gctgctgcgc cccgccatgg tcacggtcgc ccgcaaggaa aacggcaata gcggcgtcga
     1201 taccaaggcc tgatgctctg atcgtgtcgc tatcatgctt cgacaggctc agcatgaggg
     1261 agttggctgc aaccctcacc ctggtcggca cgctgtcgct atcgactgtc gaagggtgcg
     1321 gggaaacaat tccataggat aggattacgc tctaggatgg ctccatgagc gatgtgcgaa
     1381 tcgtcatagc cggagcctcg ggccgcatgg gccgggcgct ggtgcgcgtc attgccgaaa
     1441 ccaagggctg cgtgctctcc ggcgcgcttg aagccaaggg ccatcccgat ctcggccggg
     1501 atgccggcgc cctcgccggt gttggacctt tgggtgtcgt gctggcggac gatgcgtcgg
     1561 cgctgcttgc caaggccggc gcgatcctcg atttcaccgc gcccaaggct tcggtggaac
     1621 tggcgggact ggccgcggca gcgcgcgtgg ttcatgtcat cggcaccacc ggatgttcgc
     1681 cggacgacga ggccaaaatc cgcgacgccg cagggcgcac cgccattatc aaatcgggaa
     1741 acatgagtct gggcataacc ctgctcgccg ccttggtgaa gcgcgcagcc gcagcccttc
     1801 ccgctttcga tatcgagatc gtagaaatgc atcacggcag gaaagtcgat gcaccgtccg
     1861 gcaccgcctt gctgctggga gaagccgccg ccgccggacg cggcgtcgcg ctcagcgatc
     1921 acaccgtacg cgcgcgcgac ggccataccg ggccacgccg gcaagacgac atcggcttcg
     1981 ccagcctgcg cggcggcacg gtggtgggcg aacatgaagt gattctggcg ggtcctggcg
     2041 agcgcgtcgc gctgtcccat gtcgccgaag atcgcgccat cttggcgcgc ggcgccgtgg
     2101 cggcggcgct ttggggacag ggcaaaaagc ccggccttta cgccatggcc gatgtgctcg
     2161 gcctttcgga ctgatctttt cgcgccgcct cgagcgccaa tatggcgcgt ttgcgcgcga
     2221 gaccccagtg atagccggtc agcgcgccac ccgagccgag cacacgatga caaggaatca
     2281 gcaatgaaat cggattgcgc ccgacggccg cgcccaccgc gcggcaggcg cgcggggtac
     2341 agaccttctc ggcaatggtg cgataggtga cgagcttacc gctggggatc gccagcaacg
     2401 cctcccacac ttttatctgc caaggcgtgc cgatcagatg cagcggcagc acgctttcct
     2461 tttgccgttc gggatcgaaa atccgcgcgg cgattttcgc ggtgcgctct tcattctcgc
     2521 gatactccgc cttgggccag cgccccttca tatcggcgag catcgcgtct tcctcgcctt
     2581 cgtcgccgaa gcccagcccg cagacgccct tgtccgtcgc cagcaccagc accgtgccga
     2641 acggacagtc atgaaagccg taatcgatcg aaacgccgcg gccggccttg gcgtaatcgc
     2701 cgggcgtcat cgcttcgatc ttgaggcaga gatcgtgcag ccgcgacggg cccgacagcc
     2761 cggcgccgag cgccgcgtcg agaacgctgg cgccgcgcgc aagttcttcc ttggcgtgtc
     2821 cgagggtcag gtggccgaca aaggctttcg ggctgacgcc gacataacgg gtgaacgcgc
     2881 gctggaaatg aaacggcgac aggccggccg cagccgccgc gtcttcgagc cggggctgat
     2941 cgaggtaatg ctcgctgaga aagcggatgg cacggcccat gcgccgccac tgcgccgtat
     3001 cttcgccgag gattgcggtc tcactcatgg cgcgagaatg acgcaatgcg ccgtccgctg
     3061 cgacccgatt cttgcgcagc cgcgcaggca cggctggaca ggccggggtt tgccgccata
     3121 ctcgacggcg ctttttctgg agccgtcatg gcccgcccct tctccaaatc ggatgccgaa
     3181 agcttcttcg cgcggctgaa ggcggcgatg cccgaaccca agaccgaact caattatatc
     3241 aatccctaca cgctgctggt ggcggtcgtg ctctccgcgc aggcgaccga caaaggcgtc
     3301 aacaaggcga ccgaagtcct gttcaaactc gccgacacgc ccgccaagat gctcgcgctg
     3361 ggagaagccg ggctgatcgg ccacatcaag accgtcggcc tttaccgcac caaggccaag
     3421 aacgtcatcg gcttgtcgaa gattctcatg gcgcagtatg gcggcgccgt gccgcgcgcg
     3481 cgcgaggcgc tggaaagcct gcccggcatc gggcgcaaga ccgccaatgt cgttctcaac
     3541 gtcgccttcg gggaaaaaac gattgccgtc gacacccata tcttccgcgt cgccaaccgt
     3601 accggcctgg cgcccggcaa gaccccgcgc gaagtggaag acgggttgat gcgaatcgtc
     3661 ccggacgcct acgcgcttaa cgcccatcac tggctgatcc tgcacgggcg ctatgtctgt
     3721 acggcgcgcg ccccgaaatg ccccgcctgt gtcctcaacg atctttgccg ctttgccgaa
     3781 aaggtcgaac cggtcaggac cccggcgggc gggcgcgcaa agcggcaagg caattagagt
     3841 ccgtcgcggc ccttgcgccc ggcgggatgg tctcgacgtg ccggacggtg cggacatcgc
     3901 cttgcggata gaggaagaag atggcgcggc agcccgctcc atcgtagcgc cacatctcgg
     3961 cgccgttttc ctggcggacg aacgcgggcg cgccgaagaa ctggcgcaca tcctgcgccg
     4021 acaatccgac aggtctggcg gggtcctttg agggcgccag cgcggggatg ggggcggcag
     4081 gccggctggc gcaggccgag agcagcgcgc aaagacagac ggccagaacg ggacggatca
     4141 tcgatgctcc taatacgcat aacgcttgga catatgaacc atgaacgggg cgccgaataa
     4201 aggcgctccc ggatgggcgg gctggcacag gcttgcgaac ctggcgcccg tcctgtaaac
     4261 ggctttttat tggcgtaatt tcagcgggtt agggaaaaat atgacgcgca acttcgatgt
     4321 catcgcgatg ggcaacgcca tcgtcgacgt catcgctccg gtggacgatg aattcctgct
     4381 gacccacaac atcgccaagg gcatgatgac cttgatcgac gagtaccgcg ccaatcatct
     4441 ttacaacgcc ttctccgaac ggcgcgaaat cgccggcggt tcggccgcca acaccatggc
     4501 agggctggcc tcgctgggcg cccgcggaat gttcgtcggc aag
//
LOCUS       DASWPU010000137         3837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_80462,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000137 DASWPU010000000
VERSION     DASWPU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80462"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /gene="nudC"
                     /locus_tag="VGC27_03095"
     CDS             <1..530
                     /gene="nudC"
                     /locus_tag="VGC27_03095"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY0281593.1"
                     /translation="ALIDWHQRHGFCPKCGAPTVSTDAGNKRVCTACQSEHFPRTDPV
                     VIMLATKGDLALLGRNRNWPPDFFSAPAGFVEPGESIEEAVRRELFEETGVVAGAVSY
                     FASQHWPFPSQLMIGCFAACDRSDLNLDGSELAEARWFDRASAKALLAGEVKGLRGPV
                     PFAIAHHLIKAWAEK"
     gene            complement(729..1631)
                     /locus_tag="VGC27_03100"
     CDS             complement(729..1631)
                     /locus_tag="VGC27_03100"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY0281594.1"
                     /translation="MDETLISRAKRVAFQGEGGAYANLAAREALPHAEAVGKPTFEAA
                     LDAVRSGETDLAIIPVENSVYGRIADVHFLVRYFGRPIKGYVAAEQHNLFILGEHFHR
                     VRHQLLGLKGAKLAQVKTVFSQTPALGQCRKVIKELGLADKQWSDTAGAARHVAELKD
                     PSVAAIASSLAGEIYGLEVLRADVEDAPHNMTRFLIVGGEHDDAPNDGRPVITTFLFG
                     VRNVPAALYKAMGGFATNGVNVTKLESYQLRGSFNATEFYADIEGHPDQHNVRLALEE
                     LHFFSSKIVMLGIYPAHPFRAEMP"
     gene            complement(1631..2371)
                     /locus_tag="VGC27_03105"
     CDS             complement(1631..2371)
                     /locus_tag="VGC27_03105"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY0281595.1"
                     /translation="MKPIVIIPARMGSKRLPGKPLADIAGVPMIVRVWRQAVAADIGP
                     VVVAAGEKEIAQAVEHAGGRAVLTAPDLPSGSDRVWAALNAVDRGFEHDVVVNLQGDL
                     PAIEPSCLRACVDAIATTGCDITTLVAEIDDEIDAGNSNVTKAIVTWDVTKARGKAHY
                     FTRARAPWGEGPLYFHIGIYAYTRTALGRFVGLMPSPLETREKLEQLRALEAGMTIGV
                     AQVDSVPLSIDTEEDLKKARAFFAHGKT"
     gene            2599..3153
                     /locus_tag="VGC27_03110"
     CDS             2599..3153
                     /locus_tag="VGC27_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEY0281596.1"
                     /translation="MDSWEWNKIAGAVLGTLIFVLAVKFTAEAIFESPLPAKPGYVVE
                     GVIEESTTTAVAAVEEKLPDFGTVLPIADLAAGKEIEARCQQCHDSSKGGPNKIGPNL
                     WGVIGRPRAGHPGFDYSSAMAKNRDPWTYEMLFRYLKAPQADVPGTKMSFAGLRSAQD
                     RINLLAYMRMLADSPAPIPPPEKK"
     gene            3197..>3837
                     /locus_tag="VGC27_03115"
     CDS             3197..>3837
                     /locus_tag="VGC27_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0281597.1"
                     /translation="MAKPNLLMIVPAKWTDLETAAPGECAVNILLHGRDAYAPESIDY
                     VLSFRPPPGLLKSFSNLKAAFALGAGVDGFLADPAYPKRVPLVRFADRTLSAEMAQFV
                     LMHVLIRHRQQRLFDEAQAKRQWQQMMLPRRTEDTRIGMLGLGEIGSFTAARLIELGF
                     PVASWSRSRKNLPGLKSFAGEAELDAFLARTDILVCLLSLTRKTAGILNAKAFA"
BASE COUNT          686 a         1278 c         1195 g          678 t
ORIGIN      
        1 gcgccctgat cgactggcac cagcgccacg gtttctgccc caaatgcggc gcgccgacgg
       61 tttctaccga cgccggcaac aagcgcgtct gcaccgcctg ccagagcgag catttcccgc
      121 gcaccgatcc ggtcgtcatc atgctggcga ccaagggcga tctcgccctg ctcggccgca
      181 accgcaactg gccgccggat ttcttctcgg cgccggcggg attcgtggaa ccgggcgaaa
      241 gcatcgagga agcggtgcgc cgcgagctgt tcgaggaaac gggcgtcgtc gccggcgcgg
      301 tcagctattt cgcctcgcag cactggccgt ttccttccca gctgatgatc ggctgtttcg
      361 ccgcttgcga cagaagcgat ctcaacctcg acggcagcga gttggccgag gcgcgctggt
      421 tcgaccgcgc cagcgccaag gcgctgctcg ccggagaggt gaaaggcttg cgcggaccgg
      481 tccccttcgc catcgcgcat catttgatca aggcgtgggc ggagaaataa cacttgtcat
      541 tcccggccgc tcgcctacgc caaggcttcg gcgggtcatg agttgcacat tgtccgccgt
      601 agcttctgcg aaggcggaag caaagcgggg gaaaggaacc caggtgtcga cgtctgcgcg
      661 gtgtcgattc ctggttcccc ttccctcgcc gcgcgaaaag cgcggctcgc cggggatgac
      721 aagtgttttt acggcatctc ggccctgaag ggatgcgcgg gatagatgcc cagcatgacg
      781 atcttgctgg agaagaaatg cagttcctcc agcgccaggc ggacattgtg ctggtccgga
      841 tggccttcga tgtcggcgta gaattccgta gcgttgaacg aaccgcggag ctgataggat
      901 tccagcttgg tgacattgac gccgttggtg gcgaagccgc ccatcgcctt gtagagcgcg
      961 gcgggcacgt tgcgcacgcc gaacaggaag gtggtgatga ccggcctgcc gtcgttgggc
     1021 gcgtcgtcat gctcgccgcc gacgatcaga aagcgcgtca tgttgtgcgg cgcgtcttcg
     1081 acgtcggcgc gcagcacctc aagcccgtaa atctcccccg ccagcgaaga ggcgatggcc
     1141 gcgacgctgg ggtccttcaa ctccgcgaca tggcgcgcgg ccccggcggt atcggaccat
     1201 tgcttgtcgg caagacccag ctccttgatc accttgcggc attggccgag cgcaggcgtc
     1261 tggctgaaca ccgtcttcac ctgcgccagc ttggcgccct tcaatcccag caattgatgg
     1321 cgcacgcgat gaaaatgctc gcccaggatg aacagattgt gctgctccgc cgcgacatag
     1381 cctttgatcg gccggccgaa atagcgcacc aggaaatgca cgtcggcgat gcggccatag
     1441 accgaattct ccaccggaat gatcgccaga tcggtctcgc cgctgcgcac cgcgtccagc
     1501 gccgcctcga aggtgggctt gcccacggcc tcggcatgcg gcaaggcctc gcgcgccgcc
     1561 agattggcat aagccccccc ctcgccctgg aaggcgacgc gcttggcacg agaaatcagc
     1621 gtctcgtcca tcaagtcttc ccgtgcgcga aaaaagcccg cgctttcttc aaatcttctt
     1681 ccgtatcgat gctgagcggc acgctgtcca cctgggcgac gccgatggtc atgcccgctt
     1741 ccagcgcccg caactgttcc agtttctcgc gcgtttccag cggcgacggc atcaggccca
     1801 cgaagcgccc cagcgcggtg cgcgtgtagg cgtagatccc gatgtggaaa tagagcggcc
     1861 cctcgcccca cggcgcccgc gcgcgggtga aataatgggc cttgccgcgc gccttggtca
     1921 cgtcccaggt cacgatcgcc ttggtgacgt tggaattgcc ggcatcgatc tcgtcgtcga
     1981 tctcggcgac gagggtggtg atgtcgcagc cggtggtggc tatggcatcg acgcaggcgc
     2041 gaaggcaaga cggctcgatg gcaggcaaat cgccttgcag attgaccacg acgtcatgct
     2101 cgaagccgcg atcgaccgca ttcaaggcgg cccacacccg gtccgaaccg gacggcaggt
     2161 cgggcgcggt tagcaccgcg cgcccgccgg catgctccac cgcctgcgcg atctccttct
     2221 cgccggcggc gaccacgacc ggaccgatat cggccgcaac ggcctgacgc caaacccgca
     2281 cgatcatggg aacgcccgca atatcggcga gcggcttgcc gggaagccgc ttggatccca
     2341 tccgcgccgg gatgatgacg atcggtttca tctgcgcttc actccccttc gattccttgc
     2401 ggcccgctcc cgacgagcag gccggttgcg cttaccctgt acctgccgtc gcggcaggac
     2461 caaaaaatta cactaaaatt ctgcgacgct tcgccgcaac gcccctttaa gatgcgccgg
     2521 taatgtcgca tttggtcctt ccggccgagc accctcccgt ccctggcggc gcaaagcgac
     2581 acatcggagc ctgactatat ggattcctgg gaatggaaca aaatcgccgg tgcggtcctc
     2641 ggcacgctga tcttcgttct ggcggtcaag ttcacggccg aagcgatctt cgagtccccg
     2701 ctgccggcca agccgggcta tgtggttgag ggcgtcatcg aagaatccac gacaactgcc
     2761 gtcgccgccg tggaggaaaa actgccggac ttcggcaccg tcctgcccat cgccgatctc
     2821 gccgccggca aagagatcga ggcgcgctgc cagcaatgtc acgattcctc caagggcgga
     2881 cccaacaaga tcggtccgaa cctgtggggc gtcatcggcc gtccgcgcgc cgggcatccc
     2941 ggcttcgact attcgagcgc catggcgaag aatcgcgatc cctggaccta cgaaatgctg
     3001 ttccgctacc tgaaagcgcc gcaggccgac gtgccgggca ccaaaatgag cttcgccggt
     3061 ctgcgcagcg cgcaggaccg catcaacctg cttgcctata tgcgcatgct cgccgacagc
     3121 ccggcgccga tcccgccgcc cgaaaagaag tagcgccgag cttgaaccgg atcgccaaag
     3181 aggcgagcgt tccgccatgg ccaaaccgaa cctgctgatg atcgtgccgg cgaagtggac
     3241 ggacctggaa accgccgcgc cgggcgaatg cgccgtcaac atcctgctcc atggccgcga
     3301 cgcttatgcg cccgaaagca tcgactatgt gctgagcttc cgcccgcccc cgggattgct
     3361 gaaaagtttt tcgaacctga aggccgcctt tgcgctgggt gcaggcgtag acggcttcct
     3421 cgccgatccc gcttatccca agcgcgtgcc gctggtgcgt ttcgccgaca ggacgctttc
     3481 ggcggagatg gcacagttcg ttctgatgca tgtgctgatc cgtcaccggc aacagcgcct
     3541 gttcgacgag gcccaggcca aacgccaatg gcagcagatg atgctgccgc gccgtaccga
     3601 ggacacgcgc atcggcatgc tggggctggg cgagatcggc agtttcaccg ccgcgcggct
     3661 gatcgagctt ggctttccgg ttgcgagctg gagccggagc agaaagaatc ttcccgggct
     3721 gaagagcttc gcgggcgagg cggagcttga tgccttcctc gcccggacgg acattctggt
     3781 ctgcctgctg tcgctgacgc gcaagaccgc cggaatcctc aacgcgaagg ctttcgc
//
LOCUS       DASWPU010000138         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_49935,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000138 DASWPU010000000
VERSION     DASWPU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49935"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VGC27_03120"
     CDS             complement(<1..399)
                     /locus_tag="VGC27_03120"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281598.1"
                     /translation="MISARKTRANGERTLRRYRNPSDGRLHVQLTLGDTYVTSRQNEV
                     LTTILGSCIAACIRDPDAGIGGMNHFLLPNGNGRDREARCYGVNAMELLINDILKHGG
                     DRRRLEAKIFGGANVVTALSDVGTRNVAFAR"
     gene            complement(407..799)
                     /locus_tag="VGC27_03125"
     CDS             complement(407..799)
                     /locus_tag="VGC27_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0281599.1"
                     /translation="MPSASKIKAVVVDDQLTMRALVRSCLNQIGVVDVREYAGAQEAL
                     PGLKAQPAHIVLSDFNMPGMDGLEFLRAVRADPALKSIGFVLLTGRADVELVKRAVQF
                     GANNYLAKPFTVATLKQKLEQVFGPLTT"
     gene            complement(783..>1828)
                     /locus_tag="VGC27_03130"
     CDS             complement(783..>1828)
                     /locus_tag="VGC27_03130"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEY0281600.1"
                     /translation="VDDSATIRGLLTAILSRDPGIEVVGGAPDAAVARQMIKNLNPDV
                     ITLDVEMPNMNGLEFLEKIMRLRPMPVVMVSTLTQKGAEVTLSALEIGAVDYFTKPTD
                     NVAVNLGESSRELCEKVMTAAHARVRPYVRRNPPVLPVGPDFDPGRSVVAVGSSTGGV
                     EALIEVLSHFPKNCPPTVITQHMPAHFTTSFAERLNRLCAPTVSEAQDGAVLSAGKVY
                     LAPGGDSHLEIAGAGTLRCRLRNAPAAGGHRPSVDVLFESMACVARHNGIGVILTGMG
                     RDGATGLLAMRKQGAATLGQDEASSIVYGMPRVAFELGAVERQLPLEHIGPEILNLCE
                     TRVQKGVRHAISI"
BASE COUNT          322 a          569 c          598 g          339 t
ORIGIN      
        1 acgggcgaag gcgacgtttc tcgttcccac gtccgacagc gcggtgacca cgttggctcc
       61 gccgaaaatc ttggcctcga gtctgcgccg gtcgccgccg tgcttgagga tgtcgttgat
      121 aagaagttcc atcgcgttga cgccatagca tcgcgcttcg cgatcgcggc cgttgccgtt
      181 cggcagcagg aaatggttca ttccgccgat gcccgcgtcc ggatcgcgga tgcaagcggc
      241 gatgcaagag ccgagtatcg tcgtgagaac ctcgttttgg cgcgaggtga cataggtgtc
      301 gcccaaagtg agctggacat gcaaacggcc gtcgctcgga ttgcgataac gccgcagggt
      361 cctttcgccg ttcgctcgtg ttttgcgtgc ggagatcatg gcggcgtcac gtcgtcaatg
      421 gaccgaagac ctgttcgagc ttctgtttga gggtggcgac cgtgaacggc ttggcgaggt
      481 aattgttggc gccgaactgc acggcgcgtt tgacgagttc gacgtccgcc cggccggtca
      541 acagcacgaa gccgatcgat ttcaaggcgg gatcggcgcg gaccgcccgc aggaattcaa
      601 gaccgtccat gcccggcatg ttgaaatcgg aaagaacgat atgcgcgggc tgggccttga
      661 ggcccggcaa ggcttcctgc gctccggcat actcgcgcac atcgacgacg ccgatctggt
      721 tcaaacagga gcgcacgagg gcgcgcatgg tcaattggtc gtcgacgacg acggctttga
      781 tcttagatgc tgatggcatg acgaacgccc ttttgtacgc gtgtttcgca taggttgagg
      841 atttccggtc cgatatgttc gagcggaagc tgccgctcca ccgcgccgag ctcgaacgcg
      901 acgcgcggca tgccgtagac gatcgacgag gcctcgtcct gtcccagcgt ggcggcgccc
      961 tgcttgcgca tcgccaacag gccggtcgcg ccgtcgcgtc ccatccccgt aagaatgacg
     1021 ccgatcccgt tgtgtcgtgc gacgcaggcc atggactcga aaagaacgtc caccgatggg
     1081 cggtgtccgc cggccgccgg cgcgttgcgc aagcggcacc tcaacgtacc ggccccggcg
     1141 atctcaagat ggctgtcgcc gccgggggcc agatagacct ttccggcgga caagacggcg
     1201 ccgtcttgtg cttcgctcac cgtcggcgcg cagagccggt tgagccgctc cgcaaaactc
     1261 gttgtgaagt gcgccggcat gtgctgggtg atgaccgtgg gagggcaatt cttggggaag
     1321 tgcgagagca cttcgatgag ggcctccacg ccgcccgtcg aggaaccgac cgcgacgacc
     1381 gaccgcccag gatcgaaatc gggtccgacc ggcagcaccg gcgggttgcg ccggacgtaa
     1441 ggccgcacac gggcgtgcgc cgcggtcatc accttttcgc agagttcgcg cgaactctcg
     1501 ccaaggttca cggcgacatt gtccgtcggc ttggtgaaat aatccacggc gccgatttcc
     1561 agcgccgaca gcgtcacctc ggcgcccttc tgcgtcagcg tcgacaccat cacaacgggc
     1621 atgggcctga gccgcatgat cttctcgagg aattcgaggc cgttcatgtt cggcatctcg
     1681 acgtcgaggg tgatgacgtc cgggttcaga ttcttgatca tctggcgcgc cacggccgca
     1741 tccggcgcgc cgccgaccac ctcgattccg ggatcgcgcg acaagatggc cgtcagcaga
     1801 ccgcgaatgg tggctgagtc gtcgacga
//
LOCUS       DASWPU010000139         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_44073,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000139 DASWPU010000000
VERSION     DASWPU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44073"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VGC27_03135"
     CDS             <1..285
                     /locus_tag="VGC27_03135"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HEY0281601.1"
                     /translation="QADLPNTPLRAVTLDAADRARLKRGGFNDAEIDSWFGDAEFEVA
                     AGELRVTVGRAFVGNWIRNHFAQRLERAWAAEYGEVSVVVRFAAVKEKAA"
     gene            282..557
                     /locus_tag="VGC27_03140"
     CDS             282..557
                     /locus_tag="VGC27_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281602.1"
                     /translation="MSDDVSRETVCLPLNIKAQIAARAAGHRKNVRHGGLLCSHCLKA
                     PPKSANDRFCGPCRAAYERDRRKQERAELLRLRALEESLKSKERAEQ"
     gene            554..1099
                     /locus_tag="VGC27_03145"
     CDS             554..1099
                     /locus_tag="VGC27_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281603.1"
                     /translation="MTEPSEKVRKYPPVERPAPYSPTGSEPGYDGEPPEFAVRRDLVE
                     AEGKRKHQESLQNINCSRIAYLYAKKRIEWRQFMAAERLWKDAYDMGRFPKASNVIVG
                     NGASGGVMGPGDVQVNASRRYHEARSELEFARNWAIVELVVEEQVSVEKAAGRLRMHD
                     QAAMMALKLALHTLANHYRFA"
     gene            1272..1349
                     /locus_tag="VGC27_03150"
     tRNA            1272..1349
                     /locus_tag="VGC27_03150"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1307..1309,aa:Met,seq:cat)
     gene            1364..1834
                     /locus_tag="VGC27_03155"
     CDS             1364..1834
                     /locus_tag="VGC27_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281604.1"
                     /translation="MRTFKRTCIASQISGPPPTSFPIRKARFGVIGRFSFNTSYSVGR
                     DTPSRSAISVFDRFSGSQLCTSQTGVKPLDSGAQGQTCKHCGKRDAFDFHVSNETWAR
                     IVPEALQQRVVCLWCFDQFAKERGVSFAKEITTLYFAGENAVFHFTPKVVVDLA"
     gene            1818..>2121
                     /locus_tag="VGC27_03160"
     CDS             1818..>2121
                     /locus_tag="VGC27_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281605.1"
                     /translation="MSIWRDYWQFPASFFALHSLQSSPFMTTQSRIPTMPGRFKSTDM
                     RRVKPTPKQVDPFYLTPEYQAWRETVIARAGRRCEDIGLDGKRCTKAEPHHRMFADH"
BASE COUNT          475 a          593 c          634 g          419 t
ORIGIN      
        1 caggcggatt tgccaaatac gccactacgc gccgtgacgc tggatgcggc tgaccgcgcg
       61 cggctaaagc gcggtggttt caacgacgcc gaaatcgata gctggtttgg tgacgcggag
      121 ttcgaagttg cggcgggcga attgagggtg acggtcggcc gcgcgttcgt cggcaactgg
      181 atcagaaacc attttgcgca gcggctcgaa cgcgcgtggg cggcggaata tggcgaggtg
      241 agcgtagtcg tgcgcttcgc cgcagtgaag gaaaaagcgg catgagcgat gatgtttcac
      301 gtgagacggt ttgtttgccg ttaaacatta aggcacagat cgcagcgcgt gctgccggac
      361 atcgtaagaa tgtccggcac ggcggacttt tgtgtagcca ttgcctgaag gccccgccga
      421 agagcgcaaa cgatcgtttt tgtggtccat gtcgtgcggc ctacgaacgc gaccggcgca
      481 aacaggaacg cgccgaactc ttgcggttgc gggcgttgga agaaagcctg aaatcaaagg
      541 aacgggcaga acaatgacag agccgagtga gaaagttcgt aaatatcccc ccgttgaacg
      601 accggcgccc tacagcccca ccggtagtga acccggctac gatggcgagc cgccggaatt
      661 tgccgtccgg cgtgatctcg ttgaagctga gggcaaaaga aaacatcagg agtctcttca
      721 gaacatcaat tgcagccgca ttgcctatct ctatgccaaa aagcgcatcg agtggcggca
      781 attcatggcg gcagaacggt tatggaaaga cgcctacgac atgggcaggt ttccgaaggc
      841 gtccaatgtg atcgtaggta acggcgcgag tggcggcgtc atgggaccgg gcgacgtgca
      901 ggtcaatgct agcaggcgtt atcacgaagc ccgcagcgag cttgaattcg cccgcaactg
      961 ggccatcgtg gaactcgtcg ttgaggaaca agtctcggtc gaaaaggcgg ctggaaggct
     1021 gcgtatgcat gatcaagcgg cgatgatggc gctcaaactt gcgcttcaca cactcgctaa
     1081 tcattatcgg ttcgcatagc gcttgacagc ccgaaagaca attggcacta cataggcagg
     1141 attgaatcgc gcgcccgggg ctaacaaagc tgccgggcgc cgtcatatcc gaattgaata
     1201 cctggcgtac cggcacagcc cccggcatcg tgtggccccg aacggtggat caaattcggg
     1261 gaaccaattt catcgtgggg tggagcagcg gtcagctcga cggccccata agtcgtaggt
     1321 caacagttcg aatctgtttc gcgctaccaa acccaacccg tctgtgcgga cattcaagcg
     1381 gacatgcatc gccagccaga tttcggggcc gccgcccacc agcttcccga tccgcaaggc
     1441 taggttcggc gtgatcggcc gcttttcgtt caatacgtcg tacagcgtcg gccgagacac
     1501 gccgagcaga tcggcaatct ccgtcttcga ccgattttct ggttcgcaat tatgcacttc
     1561 gcaaaccggg gtcaagcctt tggatagcgg cgctcaggga caaacctgca aacattgcgg
     1621 caagcgggac gccttcgatt ttcatgtctc gaatgagacg tgggcgcgca ttgtgccgga
     1681 agcgttacag caacgagtgg tttgcctgtg gtgtttcgat caatttgcga aagagcgcgg
     1741 cgtctccttc gcaaaggaga tcaccacgct ctattttgct ggtgaaaacg cggtgtttca
     1801 ctttacgcca aaagtcgttg tcgatctggc gtgactactg gcagttcccc gcgtctttct
     1861 tcgccttgca ttcgttgcag agttcgccgt tcatgaccac acagtcccgc atccccacca
     1921 tgccaggacg cttcaagtcg acagacatgc ggcgagtaaa gccgaccccc aaacaggttg
     1981 acccattcta cttgaccccc gagtatcagg catggcgcga aacggtgatt gcccgcgccg
     2041 gccgacgctg cgaagatatc ggccttgatg gcaaacgctg caccaaggcg gaaccccatc
     2101 accgcatgtt tgccgaccac a
//
LOCUS       DASWPU010000140         8242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_56130,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000140 DASWPU010000000
VERSION     DASWPU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56130"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VGC27_03165"
     CDS             <1..1218
                     /locus_tag="VGC27_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEY0281606.1"
                     /translation="NDEPDIHSNMNPENQIKMRGPTVYGPGAFVYNTYPLVHVCGFHQ
                     RWAAAHPDKRGFILTRSGFTGIQRCQAAVWSGDVAARWSDMREQISAGVNFSLSGIPN
                     WTFDIGGYAVERRYGGYLDDVHFTKPKTKADAEEFVELYTRWYAFGAFTPIFRSHGEV
                     RRREIYEIAAPGSKVFDILKSYTELRYRLLPYIYSAASETYHQAGTMMRGLVMDFPQD
                     RKVRNIDDQYLFGRALLVAPVTEHKATGRSVYLPAGTEWYDFFTGEKFAGGQAVAVQA
                     PLGRIPVFAKAGSILPTGPVMQYTDQKPDAPLTVVVYTGKDGRFTLYEDDGISNGYRK
                     GRFATIAFAYDEAKGTLTIGPRHGNYDGMAKTRTIHVRFVSGPVAKGAKLTLDGKADR
                     TVTYDGTAVTVAR"
     gene            complement(1281..2060)
                     /locus_tag="VGC27_03170"
     CDS             complement(1281..2060)
                     /locus_tag="VGC27_03170"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY0281607.1"
                     /translation="MKTISTREKPRRSEGRRQRGKGENLTYGIVEDLGIAIITGRYGD
                     SFPVEADLCQQYGVSRSILREAVKMLTAKGLLGARPRQGTWVQPEDSWNLLDPDVLRW
                     LLERKLSFSLLIEFTEMRLAVEPIAARIAAEKASPEAKAKLCTALARMEAAQRGEDDA
                     LASDIAFHIGLLRACGNRFYGQLCELINTALHFSIRTSNRIKGVETANIQDHRRVLDA
                     ILNGDGDGAEKALRSLIQEALDLIRIAGRESLGPGKARQRR"
     gene            complement(2488..2745)
                     /locus_tag="VGC27_03175"
     CDS             complement(2488..2745)
                     /locus_tag="VGC27_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0281608.1"
                     /translation="MMVSSRQIRAARALLGWTQQTLADRALVAINTIRAIEANRPYPK
                     PDTTTAVRRALEKAGIMFLANGTMGEGVRLARPVNTNTDGN"
     gene            2858..3289
                     /locus_tag="VGC27_03180"
     CDS             2858..3289
                     /locus_tag="VGC27_03180"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0281609.1"
                     /translation="MEAKLTSLKALWDAKRQKRPMPSRDDFGIHELKPWLGHLALVDV
                     TGERIFRLCGTSLFSRFGGDMTGQPVARVSAALRRGVTDQVAKACETKTPVPVECCIQ
                     AYGQPVIYSELVLPLSQNGEEVSMLLWASYPTPQHVVHAFS"
     gene            3313..3738
                     /locus_tag="VGC27_03185"
     CDS             3313..3738
                     /locus_tag="VGC27_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0281610.1"
                     /translation="MPANLEAKDIDAHVGAQVRRRRILAGLAQQDVAKALGVSFQQVQ
                     KYEKGVNRISAGRLFILARILGTNLIEFYEGLNDEPNETTSPSTTDLKHAENFMRSRE
                     GLQLNLAFLAIKNPIMRKRMLDLIAATNESSQEIRPDKI"
     gene            4631..>8242
                     /locus_tag="VGC27_03190"
     CDS             4631..>8242
                     /locus_tag="VGC27_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HEY0281611.1"
                     /translation="MASPWDTEDSIREELFSVERLEQHAESLAAAQQITTTPIARRPL
                     AARLKDNETVLLAAYREIGNTARDGQATTPAAEWLLDNYHLVEEQIREVRDDLPRQYY
                     RQLPKLAGGPFAGYPRVFGLAWAFVAHTDSRFDADKLCRFVRAYQRIQPLTIGELWAV
                     AITLRIVFVENLRRAAKRIVSNRAARQEADSVADRLLGVNGQTAEPFGPVLQKYEHAP
                     LPAPFAVQLVQRLRDQDPNVTPALVWLEDRLTAQGITADELVHNEHQRQGAASVTVRN
                     IITSMRMASGVDWTELFESVSLVDDVLQSGSDFAAMDFPTRNLYRSAIEEMARGSHLT
                     ELEIAQAALMAASNAKEQESRARDPGYYLISGGRRSFEKTIGYHAPLRRWLARCNVRI
                     GIGGYIGSVVLVAAAALLLPLMALRTGGWTLGLLALLGSIPAMDLAVALVNRIVMAMF
                     GATALPGLALRDGIPPNLRTMVAVPTMLTTRAGIEEQIERLEVHYLASPEGDLHFALL
                     SDWTDASTEHAEEDDALLDAAAKGIDRLNVQYGQAPGGERFLLLHRRRVWNEGQGRWI
                     GWERKRGKLHELNRLLRGATDTTFMTSGGGSPAVPPDVRYVVTLDADTRLPRETVRKL
                     IGKMAHPLNRPLFDAAAGRIVEGYAVLQPRVTPSLPTGREGSLFQRIFSGMSGIDPYA
                     SAVSDVYQDLFGEGSYAGKGIYDVDAFEAALNGRVPENTLLSHDLFEGTFARAGLASD
                     VEVVEEFPSRYDVAVARQHRWARGDWQLLPWIFGRGDASRDQRGKSTLPLIGRWKMLD
                     NLRRTLSAPASVLALLLGWTMSEHDAALWTCFILSTIALPALLPVLAAIVPRRARTTA
                     RSHLDALGGDLGLALTQTALTATFLAHQAWSMTDAIGRTLFRLFVSHRNLLEWVTAAR
                     ANRSPQLRISGFYRRMTGSVVIGIASAFVVWFAGSDAWPAATPLAFLWFVAPAIARWT
                     SLSPRVAGRLPVSEADARALRLVARRTWRYFETFVTAADNMLPPDNFQEDPKPVLAHR
                     TSPTNLGLYLLSAVSARDFGWAGTTETVERLEATLATMATLQRFRGHFFNWYDTSDLR
                     PLDPFYVSTVDSGNLAGHLIAVANACRAWINRPLAPACLFAGIEDTLRLTREAIHALS
                     GGRRTEFTTRQELDSALDALGAAIGPLAATELPARLREFAKLAAAVVDITQTLASGDE
                     "
BASE COUNT         1579 a         2613 c         2509 g         1541 t
ORIGIN      
        1 aatgacgagc cggacatcca ctccaacatg aatccggaaa atcagatcaa gatgcgcggc
       61 cccacggtct atgggccggg cgccttcgtc tacaacacct atccgctcgt tcatgtctgc
      121 ggcttccatc agcgctgggc cgctgcccat cccgacaagc gcggcttcat cctgacgcgc
      181 tccggcttta ccgggatcca gcgctgccag gcggcggtgt ggagcggcga cgtcgccgcg
      241 cgctggagcg acatgcgcga gcagatttcg gccggcgtca atttctcact gtccggcatc
      301 cccaactgga cgttcgacat cggcggctac gcggtcgagc ggcgctatgg cggctatctc
      361 gacgatgtcc acttcaccaa gccgaaaacc aaagcggatg ccgaggaatt cgtcgaactc
      421 tataccaggt ggtacgcgtt cggcgctttt accccgatct tccgctcgca tggcgaggtg
      481 cgccgccgcg agatctacga gatcgctgcg cccggcagca aggtgttcga tatcctgaaa
      541 tcctacaccg aactgcgcta ccggctgttg ccctacatct actccgccgc gtccgagacc
      601 tatcaccagg ccggcacgat gatgcgcggc ctggtcatgg atttcccgca ggatcgcaag
      661 gtgcgcaaca tcgacgacca gtatctgttc ggccgcgcct tgctggtggc gccggtgacg
      721 gagcacaagg cgaccggccg cagcgtctac ctgccggcgg ggacggaatg gtacgacttc
      781 ttcaccggcg agaaattcgc cggcggccag gccgtcgcgg tgcaggcgcc gctgggccgc
      841 attccggtct tcgccaaggc gggcagtatc ctgccgaccg gcccggtcat gcagtatacg
      901 gatcagaagc cggacgcgcc gctcaccgtc gtcgtctaca ccggcaagga cggccgcttc
      961 accctgtacg aggatgacgg catctcgaac ggctatcgca aaggacgctt cgccaccatc
     1021 gccttcgcct atgacgaagc gaaaggcacg ctcaccatcg gcccgcgcca cggcaattat
     1081 gacggcatgg cgaaaacgcg gacgatccat gtccgcttcg tttccggccc cgtggccaag
     1141 ggcgcgaagc tcacgctcga tggcaaggcg gacaggaccg tcacctatga cggcacggcg
     1201 gttactgtcg cacgctgagc gggacgcatg aaaaagaacg gcgcgggggg cctctcgcgc
     1261 cgtttctttt tttgcgccgg tcagcggcgc tggcgcgcct tccccggccc cagggattcg
     1321 cgtccggcga tgcggatgag gtcgagcgcc tcctggatca ggctgcgcag cgccttttcg
     1381 gcgccgtcgc cgtcgccgtt caggatggcg tcgagcacgc ggcgatggtc ctggatgttg
     1441 gcggtttcca cgcctttgat gcggttggag gtgcggatcg agaaatgcag cgccgtgttg
     1501 atgagttcgc agagctggcc gtagaagcgg ttgccgcagg cgcgcaagag gccgatatgg
     1561 aaggcgatgt cggaggcgag cgcatcgtct tcaccgcgct gagccgcctc catgcgggcc
     1621 agcgcggtgc agagcttggc cttggcttca gggcttgctt tttcggcggc gatgcgtgcg
     1681 gcgatcggct cgaccgccag gcgcatctcg gtaaattcga tgagcagcga gaaggagagc
     1741 ttgcgctcca aaagccaacg cagcacgtcg ggatcaagca ggttccagct atcctccggc
     1801 tgcacccagg tgccctggcg cgggcgcgcg cccaagagcc ccttggcggt gagcatcttc
     1861 accgcctcgc gcaggatgga gcggctgacc ccgtattgct ggcacagatc ggcctcgacg
     1921 ggaaaggaat cgccatagcg cccggtgatg atggcgatgc ccagatcctc gacaatgcca
     1981 taggtgaggt tttcgccctt gccgcgctgc cggcggcctt cgctgcggcg cggtttctcc
     2041 ctagtcgata tcgttttcat ccggcaatcc cgtcaccccg gtttatacac cagtacgtct
     2101 cctggcccag ggtgcctgaa agacatgtgc ccgaaagacg cattgtcctc atccgccgac
     2161 gcgacgaaca acagaccagg ccgggccccg ccgaaggcgg cacaacccgg ccggcgcacc
     2221 ggcaccgcga cgatagcgga aaccgtgcca tccggtgcat agcggacgat ccgccctcca
     2281 tccctgtcgg cgctccacag atggccttcg ccatcgaccg cggcgagtcg gcaaagtaca
     2341 gtcgcgtgcc gtccgggctg acggcaaggc cattggcgat tacgacaccg gtagcataac
     2401 aggaaacttg ttccttctgg tccagacagt ggagggcgcc cagggccggg ccgcggattt
     2461 catcccgtcg gatgcacttg cagcgaatca atttccatcc gtgttcgtat tcacgggacg
     2521 cgccaatcga acaccttcac ccatggtgcc gtttgccagg aacataatgc cggccttttc
     2581 aagtgcccgg cgcactgcgg tcgtcgtgtc aggcttcggg tagggacgat tcgcttcgat
     2641 cgctcggatc gtattgatgg cgaccaaggc gcgatccgcc agcgtttgct gcgtccaacc
     2701 gagaagtgcg cgcgccgcgc ggatttgccg tgaggagacc atcatggtct acctgaagca
     2761 tcaaatggac gggcgctatt gatttggtta taataactaa atctagcgac tagcgaccga
     2821 ttcggctgcc gcctccgcaa cacgccgggt gaatttgatg gaagccaaat tgacaagcct
     2881 gaaagcgctt tgggatgcca agcgccagaa gcggccaatg ccgtcgcggg atgacttcgg
     2941 cattcacgaa ctcaagccct ggctgggcca tttggcactg gtcgatgtca ccggggagcg
     3001 gatttttcgt ctatgcggca caagtctctt ttcccgattc ggcggggaca tgacggggca
     3061 accagtcgca cgggtcagcg cagccctgcg acggggagtg acggaccaag tcgccaaggc
     3121 atgcgaaacc aaaacgcccg ttcccgtgga gtgttgtatc caggcgtatg gtcagccggt
     3181 catctattcg gaactggtcc tgccactgtc tcagaacggc gaggaggtga gtatgttgct
     3241 ctgggcaagc tatccaacac cgcaacatgt ggttcacgcc ttctcgtgag aagcgccaag
     3301 tcgagacata aaatgccagc caaccttgag gccaaggaca tagatgctca cgtcggcgca
     3361 caagtacgac ggcgccgaat actcgcgggt ctcgcccaac aggatgtcgc gaaggcgctt
     3421 ggggtcagct ttcagcaggt gcagaaatat gagaaaggcg tcaaccgcat cagtgccggg
     3481 cgcttgttca ttctcgcccg aattctagga acgaacctga tcgagtttta tgaaggactg
     3541 aatgacgagc caaacgaaac aacaagcccc tccacgaccg accttaaaca cgccgaaaac
     3601 ttcatgcgaa gccgcgaagg gcttcaactg aacctggctt ttctcgcgat caagaacccg
     3661 attatgcgaa aacgcatgtt agacctaatt gccgcgacaa atgaatcatc gcaggaaatt
     3721 aggccggaca aaatctagtg tcccgattcc gaaattcgct ccctctctat atcggcctcg
     3781 ggcgaatttc ggaatcgaaa cgacactagc gcgatataag acaaagaata taccgtcagg
     3841 cagcgaacca gcgccgccag attgcccagt gtttgttgaa aacttccaga acgaattttg
     3901 accgtatccg cctcatcata atcggtaatt atgagtagtt tctcattatc tttgcgggcg
     3961 ccgccgatgt aggggaggca ggatcgaaga acgcccctct cagaagaaaa ggtgcgcgcc
     4021 aatcggtaac atcaggttct tgcacgatgc gcgtaaatct cttcccatgt cgccgcgttc
     4081 cagccttcgg cataaccatg gctcagacga aatgcgccgc cgctcatggt cacgtcacct
     4141 catagacaat cacaaagcag attgacggcc ttcgagcctt tggcatccgg ctggatgtcg
     4201 aacgacacct gaaggccgat acgctggtcg cttgcaaatc ggcagaaaca gcacgccgga
     4261 cggcggaacg gccggcattg acgaagatcg gcgccgttgc gttttccggt tcgggggacc
     4321 ggtaaccggt cgaagatgac gataaacccg tcattatctt caaaaacagc cgattatcgc
     4381 agcaatttga agatttaggc gtgacaggat atcggtatat aaggtgttat aatcttccga
     4441 ccgactctcg ccgtggccct ggaatacgtc ctgcgttctt cggcatggag tccattccgc
     4501 gaagtttgag cgagatgcgt ggccacaacc gggcatcgca tgcgccggct tggtggttca
     4561 aattccgtgc gcccgcggca gaaaggccaa attgatattg tcgttccttc cagtcttcgc
     4621 ccaaagcccc atggcctcgc catgggatac ggaagactcc atccgcgagg agcttttcag
     4681 cgtcgagcgc ctggagcagc acgcggagag ccttgccgcc gcccaacaaa tcaccaccac
     4741 accgatagcg cgccgaccgc tggcagcgcg gctgaaggac aatgaaacag tcctgcttgc
     4801 ggcatatcgc gaaatcggaa acacggcccg cgatgggcag gccacgacgc ccgccgccga
     4861 atggctgctc gacaattatc atcttgttga agagcagatt cgtgaagttc gagacgatct
     4921 gccccggcaa tattatcgcc agttgccgaa gctggcgggc ggcccctttg ccggctaccc
     4981 gcgcgtcttc gggctcgcct gggcgttcgt tgcccatacc gacagccgct tcgatgccga
     5041 caagctgtgc cgcttcgtgc gcgcctacca acgcatccag ccgctcacca tcggcgagtt
     5101 atgggccgtc gcgatcacat tgcgcatcgt gtttgtcgaa aacctgcggc gcgcggccaa
     5161 gcgcatcgtg tccaaccggg ccgctcgcca ggaagcggat agtgtggccg accggcttct
     5221 cggggtgaac gggcaaactg ccgagccctt tggcccggtg cttcagaaat acgaacacgc
     5281 gcctttgccg gcgccctttg ccgtccaact cgtccaaagg ctgcgggacc aggaccccaa
     5341 cgtcacgccg gctctcgtat ggctggaaga ccgcctgacg gcccaaggca tcacggcgga
     5401 cgaactcgtg cacaacgagc accaacgcca gggcgcggcg agcgtgacgg tgcgcaacat
     5461 catcaccagc atgcgcatgg cgtccggcgt ggattggacg gagctgttcg agagcgtcag
     5521 tctggttgac gacgtgctgc aatccggcag cgatttcgcc gccatggatt tcccgacgcg
     5581 caatctttat cgcagcgcga tcgaagagat ggcgcgcggc tcccacctga ccgaactcga
     5641 aatcgcgcaa gccgcgctca tggcggcgag caatgcaaag gagcaagaaa gccgcgcgcg
     5701 cgatcccggc tattatctga tttccggcgg gcgccgctcg tttgagaaaa caatcggata
     5761 tcacgccccg ctgcgccgct ggcttgcacg ctgcaatgtg aggatcggca tcggcggcta
     5821 tatcggcagc gtcgtcctgg ttgcggcggc ggcgttgctc ctgccgctga tggcgcttcg
     5881 gaccggcggc tggacgctcg gcctcctggc gcttctcggc tcgatcccgg ccatggacct
     5941 ggcggtcgcg ctggtgaacc ggatcgtgat ggccatgttc ggcgcgacgg ccctgcccgg
     6001 cctggcgctt cgcgatggca taccgccaaa cctgcgcacg atggtcgccg ttccgaccat
     6061 gcttacgaca cgagcaggga tcgaagaaca aatcgaacgc ctggaagtcc actacctcgc
     6121 gagtccggag ggcgatctcc atttcgcgct gctctcggat tggacggacg cctcgacgga
     6181 acatgccgag gaagacgacg cgctcctcga tgcggccgcc aagggcatcg accgtctgaa
     6241 cgtccaatac ggtcaggcgc cgggaggcga acgcttcttg ttgctccatc gccggcgagt
     6301 ctggaacgaa gggcaaggac gatggatcgg gtgggaacgc aagcgcggca agcttcacga
     6361 attgaatcgc cttctgcgcg gcgcgaccga taccacgttc atgacgagcg gcggcggatc
     6421 gcccgccgtg ccgccggatg tccgctatgt cgtcaccctc gatgccgaca cgcggctgcc
     6481 tcgggaaacc gtccgcaagc tcatcggcaa gatggcgcat ccgctcaacc ggccgctgtt
     6541 cgacgccgcg gccggacgca tcgtcgaggg atatgcggtg ctgcagccgc gcgtcacgcc
     6601 gtccctgccg accggccgcg aaggatcgct gttccagcgc atcttttccg gcatgagcgg
     6661 catcgaccct tatgcctcgg ccgtttccga cgtgtaccag gatctgttcg gcgaagggtc
     6721 ctacgccggc aagggaatct acgatgtgga tgcgttcgag gccgcgctga acggccgtgt
     6781 gccggagaac acgcttctca gccacgatct tttcgaggga accttcgccc gcgccggcct
     6841 ggcgtcggac gtcgaagtgg tcgaggagtt tccctcccgc tatgacgtcg ctgtggcgcg
     6901 ccagcaccgc tgggcccgcg gcgattggca gctcctgccg tggattttcg ggcgcggcga
     6961 cgcatcgcgc gatcagcgcg gcaagagcac gcttccgctt atcggacgct ggaagatgct
     7021 cgacaatctg cggcggacgc tctccgcacc ggcaagcgtc ctcgcgttgc tgctcggctg
     7081 gaccatgtcc gagcatgatg ccgcgctatg gacctgcttc atcctatcga ccattgcact
     7141 gccggccctg ttgccggttc tcgctgccat cgtgccgcgg cgcgccagga cgacggcccg
     7201 aagccatctg gatgcgctcg gcggcgatct cggtcttgct ttgacgcaaa cggcgttgac
     7261 ggcaacgttc ctggcccatc aggcgtggtc gatgaccgat gccatcggca ggacgctgtt
     7321 ccgcctcttt gtgagccatc ggaatttact cgaatgggtc acggcggcgc gcgcgaatcg
     7381 aagcccgcag ctgcgaattt caggtttcta tcgccggatg accggcagtg ttgttatcgg
     7441 catagcgtcc gcgttcgtcg tttggttcgc cggcagcgat gcgtggccgg ccgcgactcc
     7501 cttggcgttt ctctggttcg tcgccccagc gatcgcgcga tggacgagtc tttctccccg
     7561 tgtggccggc cgtcttccgg tgtcggaagc cgatgcacgc gccttgcgat tggtcgcacg
     7621 ccggacctgg cgctacttcg agaccttcgt cacggccgca gacaacatgc tgccgcccga
     7681 caacttccag gaagacccaa aaccggtcct cgcgcaccgg acgtctccca ccaatctcgg
     7741 cctttatctt ctttcggcgg tcagcgcccg cgatttcgga tgggcgggca cgaccgagac
     7801 ggtcgagcgc ttggaggcca cgctcgcgac aatggcgacg ctccaacgct ttcgcggcca
     7861 cttcttcaat tggtacgata cgagcgattt gcgcccgctt gatccgttct atgtctccac
     7921 cgtggacagc ggcaatctcg cggggcacct gatcgcggtc gcgaatgctt gccgggcatg
     7981 gatcaaccgc ccgctcgcac ccgcctgcct gtttgccggc atcgaggata cgttacgctt
     8041 gacgcgtgaa gccattcacg cgctttcagg cggccgtcga acggagttca ccacaaggca
     8101 agagcttgac agcgcgcttg acgcccttgg cgcagccatc ggtcctctgg cggcaaccga
     8161 actgccggca cgactcagag aattcgcgaa actggccgcg gccgtggtcg atatcacgca
     8221 aacgctggcc agcggggatg aa
//
LOCUS       DASWPU010000141         4568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_37982,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000141 DASWPU010000000
VERSION     DASWPU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37982"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VGC27_03195"
     CDS             complement(<1..541)
                     /locus_tag="VGC27_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0281612.1"
                     /translation="MTGSDIAISVENLSKRYLVGHETARREGYQTLRETLMRNVRNFG
                     RKTMDMARGRQILQGDEIEEFWALKDVSFEVKRGEVVGIIGRNGAGKSTFLKILSRIV
                     SPTAGRAELHGRVASLLEVGTGFHPELTGRENVYLNGAILGMTKSEIRRRLTDIVDFS
                     EISAFIDTPVKRYSSGMYVR"
     gene            complement(546..1376)
                     /locus_tag="VGC27_03200"
     CDS             complement(546..1376)
                     /locus_tag="VGC27_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0281613.1"
                     /translation="MKQTLETVLEPGRAERNYWRDLWRYRELFQVLAARDVAVRYKQT
                     VIGVVWAVLRPFLTMLIFTVIFSRLAKLPSEGEAPYALMVLAGLLPWTFFSTALGDAS
                     ISLVTNSNLIGKVYFPRLIIPTATIVVSFVDLMISFAILVVMMIWYSFLPSWHIAVLP
                     VFVVLAFLASLGPGLWITALNVKYRDFRYVIPFIIQFGLYVSPVGFSSSVVPEKWRLL
                     YSLNPIVGVIDGFRWCVLGGASRIFWPGLMLSLAIIAFFLWLGIRQFRKMEKTFADLI
                     "
     gene            complement(1391..2371)
                     /locus_tag="VGC27_03205"
     CDS             complement(1391..2371)
                     /locus_tag="VGC27_03205"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281614.1"
                     /translation="MIISRTPLRVSFFGGGSDLPAYYRQHGGAVLSTAIDKSVYVTVS
                     QKFDDAVRVSYSRTEEVARASSVEHPLVREALVMLGIEGGIEITSVADIPATGTGLGS
                     SSSFTVGLLNALHAYCGRHATATDLAQEGCRIEIERCGEPIGKQDQYAAAFGGFNFIR
                     FHPDETVEVKKAVCPRNVLDDLQSRLLFFYTGITRSASALLQAQSAKVARQGEKSDAT
                     GELVRLAETAFGELCAGKIETFGPLLHEAWQIKRQLTDGVTNSVIDEAYRAATEAGAE
                     GGKILGAGGGGFLMFFAPRERHDAIRGALHRLRETPFAFAPHGSSIIFVH"
     gene            complement(2368..3705)
                     /locus_tag="VGC27_03210"
     CDS             complement(2368..3705)
                     /locus_tag="VGC27_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEY0281615.1"
                     /translation="MTHPPAKADHLVIVAGGKGTRLAAQFQDLPKVLVPIGGKPVLAH
                     QLELAAAAGIRTVTIFAAHLAEKIQSFVGDGSRFGPNIRILVEKEPLGNAGALLRSLD
                     MLPAEFFLLYGDVMAAVDLRRMARKHLERGADVTALAHPNDHPYDSDLLETDADDWVT
                     AVQPNPHPADRFFANLVNAALYVVRRDALLPWAGQSGKSDFAKDIMSALAAGGGRILA
                     YRSGEYIKDMGTPDRLRRVEADWRDGKIDIAQPHRPRPAIFLDRDGTLNVEKGFLRAA
                     EDLELAAGVGAALKTLRQAGFLLVVLTNQPVIARGEASEADVAAIHRRLEWELGKEGA
                     YLDRIYFCPHHPDRGFPGERADLKIDCECRKPATGLFRRACEELDIDAANSWMIGDQT
                     RDIEMARRAGLRSVLVRTGVAGGDKKFPAEPDHIAGSLSAAADFIVKQEVSVS"
     gene            complement(3706..>4568)
                     /locus_tag="VGC27_03215"
     CDS             complement(3706..>4568)
                     /locus_tag="VGC27_03215"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0281616.1"
                     /translation="LGRPEHLQSARTNSGFRFFERDVSRNEQAVECLRAASDWAGPPD
                     IVWHLAANSDIAAGALDASVDFKRTLQTTFAALEAAKSVGAKKFAFASSSAVYGERDD
                     LLTEDSGPLLPISNYGATKLASEALISAAAEAYLDRIWLFRFPNVVGPRATHGAIFEF
                     IKHLVPGAANLKVLGDGSQTKPYLHVSELIEAMTYIVAKAEGRRNLFNIGPSGEGTSV
                     AFIAEQAVARVLPGTPIVYTGGDRGWIGDVPRFRYSNERLARLGWQPKLSSDEAVLRA
                     IGEIAGAFGR"
BASE COUNT          914 a         1430 c         1413 g          811 t
ORIGIN      
        1 ggcggacgta catgccgctc gaatatcttt tgaccggcgt gtcgatgaac gccgaaatct
       61 cggaaaagtc gacgatgtcg gtcagtcgcc ggcgaatctc ggatttagtc atccccagaa
      121 tcgcgccgtt cagatagaca ttttcgcggc cggtcaattc cggatgaaat cccgtgccga
      181 cttccagcag gctggcgacg cgaccgtgca gctccgcccg gcctgccgtg ggggagacga
      241 tgcgcgaaag aatcttcaga aacgtcgatt tgcccgcccc gttgcggccg atgatgccga
      301 cgacttcgcc gcgcttgacc tcgaacgaga catccttcag cgcccagaat tcctcgatct
      361 cgtcgccttg caggatctgg cgcccgcgcg ccatatccat ggtcttgcgg ccgaaattcc
      421 tgacgttgcg catcagcgtt tcgcgcaacg tctgataacc ctcgcggcga gcggtttcgt
      481 gcccgacgag atagcgcttg gaaaggtttt cgacgctgat cgcgatgtcg ctgccggtca
      541 tggtctcaaa tcaaatccgc gaaggtcttt tccattttgc ggaactggcg gattcccagc
      601 cacaggaaga atgcgatgat ggcgagactg agcataaggc ccggccagaa gatacggctc
      661 gccccgccca agacgcacca gcgaaaaccg tcgatgacgc ccactatcgg attgagcgag
      721 tagagcaagc gccacttctc cggcacaacg ctcgaactga accccaccgg cgagacgtag
      781 aggccgaatt gaatgatgaa cggaatgacg tagcgaaagt cgcggtactt gacgttgagc
      841 gccgtgatcc acagaccggg accgagcgag gcgagaaacg ccagcaccac gaaaaccgga
      901 agcaccgcga tgtgccaact cggcaggaag ctgtaccaga tcatcatcac gacgaggatg
      961 gcgaagctga tcatgagatc gacgaacgag acgacgatcg tcgccgtggg aatgatcagc
     1021 cgcggaaagt agaccttgcc gatgaggttg gaattggtga cgagactgat cgacgcgtct
     1081 cccagagcgg tcgagaaaaa cgtccacggc agcagacccg cgagaaccat cagcgcatag
     1141 ggcgcctcgc cttcgctggg caatttcgcc aggcgcgaga agatcacggt gaaaatgagc
     1201 atcgtcagga acggccgcaa gaccgcccac acgacgccga taaccgtctg cttgtagcgc
     1261 accgcgacgt cgcgcgccgc cagcacctgg aacaactcgc gatagcgcca aagatcgcgc
     1321 caatagttgc gttccgcgcg acccggttcg aggacggttt ccaatgtctg tttcatggga
     1381 gcgggtttgg tcaatggacg aagatgatgc tgctgccgtg cggcgcgaag gcaaagggcg
     1441 tttcgcgcaa cctatgaagc gccccgcgga tcgcgtcgtg gcgctcgcgc ggcgcgaaaa
     1501 acatcaggaa gcctcccccg ccggcgccca gaatcttccc gccttcggcg cccgcttccg
     1561 tcgcggcccg ataagcctcg tcgatcacgg aattggtgac gccatccgtc aattgacgct
     1621 ttatttgcca tgcttcatgg agcaacggcc cgaatgtttc gatttttccc gcgcaaagct
     1681 cgccgaaggc ggtttccgcc aagcggacca actcgccggt agcatcggat ttctcgcctt
     1741 gccgggccac cttggcggat tgcgcctgca agagagcgga ggccgagcgg gtaatgccgg
     1801 tgtagaagaa caaaaggcgc gactggagat cgtcgagaac gttgcgcggg cacaccgcct
     1861 tcttcacttc cacggtctcg tcggggtgaa agcggatgaa attgaaaccg ccgaacgcag
     1921 cggcatattg atcctgctta ccgatgggtt cgccgcaacg ttcgatctcg atgcggcagc
     1981 cttcctgggc gagatccgtc gcggtcgcgt gccggccgca atacgcgtgc agcgcgttca
     2041 gcagtcccac ggtgaaggaa ctggacgatc ccagccccgt ccccgtagcg ggaatatccg
     2101 caaccgacgt aatttcgatg ccgccttcga tacccaacat gacgagggct tcgcgcacca
     2161 gcggatgctc gacagaagag gcgcgcgcga cctcttcggt gcgcgaatag cttacgcgga
     2221 cggcgtcgtc gaacttttgg ctcaccgtga cgtagaccga tttgtcgatc gcggtggaca
     2281 gcacggcccc gccgtgttgc cggtaataag cgggaagatc ggagcctccg ccgaaaaagc
     2341 tgacacgcag cggtgtgcgg gaaatgatca cgagactgaa acttcctgct tcacgatgaa
     2401 atccgcggct gcggaaagac tgccggcgat atgatcgggc tcggccggga atttcttgtc
     2461 cccgccggcc acgccggtgc gaaccagaac cgagcgcagg cccgcgcggc gcgccatctc
     2521 gatgtcgcgc gtctgatcgc cgatcatcca ggaattcgcg gcgtcgatgt ccagttcttc
     2581 gcaagcgcgc cggaacaggc cggtcgccgg cttgcggcac tcgcagtcga tcttgagatc
     2641 ggcgcgctcg ccgggaaacc cccggtccgg atgatgcggg cagaaataga tgcggtcgag
     2701 ataggcacct tcttttccaa gctcccattc cagacggcga tggatcgcgg caacgtcggc
     2761 ctcgctcgcc tcgccgcggg cgatcacggg ctggttggtc aacaccacga ggagaaagcc
     2821 cgcctgccgc aaagttttaa gcgccgcgcc caccccggca gcgagttcca aatcctcggc
     2881 cgcccgtaga aacccctttt cgacattcag cgtaccgtcg cgatccagaa agatcgcggg
     2941 ccgcggccgg tgcggctggg cgatgtcgat tttgccgtcg cgccagtccg cttccacgcg
     3001 ccggagcctg tccggtgtgc ccatgtcctt gatgtattcg ccggagcggt aggcgagaat
     3061 ccgcccgccc ccggccgcca acgcggacat gatgtctttg gcgaaatcgg acttgccgct
     3121 ttgcccggcc cagggaagca gggcgtcgcg ccgaaccaca tacagcgccg cgttcaccag
     3181 gtttgcgaaa aagcgatccg ccggatgcgg attgggctga acggccgtta cccagtcgtc
     3241 cgcatcggtc tccagcagat cggaatcgta aggatggtcg ttcggatggg cgagcgcggt
     3301 gacgtcggcg ccgcgctcca gatgtttgcg cgccatacgc cgcagatcca ccgccgccat
     3361 gacatcgccg tagagaagaa aaaattcggc gggcagcata tccagggacc gcaacaaggc
     3421 gcccgcgttt cccagaggtt ccttttccac caggatgcga atgttcggac caaagcgcga
     3481 accgtcgccg acgaagctct gaattttttc ggcaagatgc gcggcgaata tcgtcaccgt
     3541 cctaattccc gcagccgccg ccagttcgag ttggtgcgcg agaaccggtt tacctccgat
     3601 gggcacgaga accttgggca ggtcttggaa ctgagccgcc aggcgcgtgc ccttgccgcc
     3661 ggcaacgatg acgaggtgat cggctttcgc cggcggatgc gtcatctagc ggccaaatgc
     3721 accggcgatt tcgccgatgg cgcgcagcac cgcctcatcc gagctgagct tcggttgcca
     3781 gccaaggcgc gccagccgtt cgtttgagta gcggaatcgc ggcacatcgc cgatccatcc
     3841 ccggtcgccg cccgtataga cgatcggcgt tcccggcaga acacgggcga ccgcctgctc
     3901 ggcgatgaaa gcgacgctgg tgccctcccc gctcggtccg atgttgaaca ggttgcggcg
     3961 cccctctgcc ttcgcgacga tgtaggtcat cgcctcgatg agttccgata cgtggagata
     4021 gggcttggtt tgcgacccgt cgccgagaac cttgaggttt gcggcgcccg gcacgagatg
     4081 tttgatgaat tcgaagatcg cgccatgcgt cgcccttggc ccgacaacat tggggaaccg
     4141 gaacaaccaa atcctgtcca ggtacgcttc ggctgccgcg ctgatcaatg cctcgctggc
     4201 cagtttggtc gcgccgtagt tggagatggg cagcagcgga ccggaatcct cggtcagcag
     4261 atcgtcgcgc tcgccgtaaa ccgccgacga ggatgcaaag gcgaatttct tcgcgccgac
     4321 ggatttcgcc gcttcgagcg cggcgaaggt ggtctgcaac gtccgcttga agtcgaccga
     4381 ggcatcgagc gcgccggcgg cgatatcgct gttcgccgcc aggtgccaaa cgatatcggg
     4441 cggcccggcc caatccgacg cggcgcgaag gcattcgacc gcctgttcgt tgcggctgac
     4501 gtcgcgctcg aagaaacgaa aaccggaatt tgtgcgcgcg gattgcagat gttccggcct
     4561 gccgagcg
//
LOCUS       DASWPU010000142         6705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_49940,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000142 DASWPU010000000
VERSION     DASWPU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49940"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VGC27_03220"
     CDS             complement(<1..924)
                     /locus_tag="VGC27_03220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0281617.1"
                     /translation="MPRPLNQTSNMQPRSRPKVVHVVVAGEIGGAERFLVNLATRPEL
                     SGADHSIALMTPNPKLRAFFTDAGVRIYDRGPVRENPLAYLWRTWGPLDIAWLGRVLK
                     EEGADLLHTHTHGALVLAARTGLRYALPVLRTEHNVRHYQDPSCALFRHWALRHTTRV
                     AAVSAHVAKAAASIAPHARDHIQVILVGIDTTWFTPAPPPPEGPFTFAIVCRLEPVKQ
                     VSVAIEAVAKTPGVRLDIVGEGSERARLESLVKQRGVEDRVRLLGFVSDQRPVVAAAD
                     AVINCSRAEGLPASLMEAAAQQRPAVAFDCCG"
     gene            1168..1884
                     /gene="lptB"
                     /locus_tag="VGC27_03225"
     CDS             1168..1884
                     /gene="lptB"
                     /locus_tag="VGC27_03225"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY0281618.1"
                     /translation="MLSVQHLVKSLAGRRVIEDVTLSLERGEIVGLLGPNGAGKTTTF
                     RMLTGLVIADAGRIELDGADITNLPFYERARRGVSYLPQESFVPRSLSVEDNIAMVLE
                     VRERSAKKRSEKLDTLISQFHLDELRRVPVGTLSSGQRRRCEIAITLACEPGFALLDE
                     PFARVDPNAIEEITVLIRHLTDRGIGVLITDHNARELFQLVDRAYVIENGRVLAHGSA
                     EELIADLNVRQAYLGEAFRI"
     gene            1881..3176
                     /locus_tag="VGC27_03230"
     CDS             1881..3176
                     /locus_tag="VGC27_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY0281619.1"
                     /translation="MMWKPAAWIANLHRRILKAWLRTVCAPFLHLYARNFFAGVALLV
                     FTIMALVEAVFLAERFPMVFREVFRHHADPLDTTLLFLCNSTQVFDLALAIAILMAVY
                     WTTLRMRENRELLVLFAAGTGPYRLLALVLVIAVAAQIGSLTISGVLDPAARYAQRVI
                     LFNAEFRALRSGINTGQFYSFPGRVAFAPARPAERDRAEPERTRGLFVYEDLKPGKFR
                     VITADHARLDGPDASGKVVLRLGGLTALTFSDEPPAAGAGPAPAKTGAVACTDCPVQP
                     SGVPRMTLSASDVVQEMASDQLLTFMPRGSKSEEMTIFEQFAATSDSSSLRHREDMRL
                     LGGRLARSFLCLLAPLVALACICFTTRATHYFALPIACMGLMSLNVTSEWLVRVTVPL
                     NPLGALSVPAALTAVFAALLLTEIFREQGMLARPQLARS"
     gene            3173..4393
                     /locus_tag="VGC27_03235"
     CDS             3173..4393
                     /locus_tag="VGC27_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY0281620.1"
                     /translation="MSINTLSGPEKRAPRAIRFSPMGRHTRYMLAAYLRNVFMVISVL
                     LAIALTIDLWPQFQIIAGDQGDGALAATWRVLRFSALRTPGLVAPFLPFATFLGVVWT
                     EVVHTQSGERMLVWNSGRSPIQCLAPAFLLGVILGAGEFAMDAYLGPAAMSVQMHERL
                     GLDGQRLDRTRIRSASWIALPDGLLRAEIEYGPPAVLHRLTSFKRDAAGQLTEVDMAA
                     VARRQPGTNLWLLHDGRSWSAKGATAQDDASLPVVALGGFTNETMVPFKTRTISLDLD
                     PLWLSVFGMETQYLPLHVLRQLVRSDRDSDSRTSYRTRLQVLYGEAFLPGAMALLAAS
                     LSLLLLAYATRATALVGIVFVGYMAHFGTKACLLMGQTGFLPPAVAGWFVPAGLLVAT
                     LAVLGVIERQRRRI"
     gene            complement(4420..4935)
                     /locus_tag="VGC27_03240"
     CDS             complement(4420..4935)
                     /locus_tag="VGC27_03240"
                     /inference="COORDINATES: protein motif:HMM:NF027443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4833 domain-containing protein"
                     /protein_id="HEY0281621.1"
                     /translation="MTSQVTELEQIPRVRPEFPVPNEPNMLFYIERSVNSNTVVYAAN
                     LDAQGHIVADEPVIAYWRWYNRGGHRKALNLAERMMAYGVKSVKRNKADGTFTFKIAA
                     LPERTLYVGLDGKGQPEAFGKVGNRWVKLVYVYLEVDDSGLLPDVTALDFFGYDKLTG
                     KPLREHVVPHK"
     gene            complement(5063..6280)
                     /locus_tag="VGC27_03245"
     CDS             complement(5063..6280)
                     /locus_tag="VGC27_03245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0281622.1"
                     /translation="MKIVDISEFYSPTGGGVRNYVDHKFKAAARHGHCLTVIAPGTES
                     RVEAREGGKLVWVETPQLPFDRNYRMFWRAAEVLRIVDGETPDVVEGSSPWRGGWIAG
                     QWRGPAVKMLFMHADPVAVYPQMLLSGLLSPSGIDTLFAWYWSYLRRLNARFDGCIVA
                     GSWLATRFAGHGLRNLHVVPFGVETKWFGPGLRDLTLRTAMLAKCGLGPGAALLVNIG
                     RHHPEKRVGTAIDAVTKAQKSRPVGLYVIGDGFIHTSIEAKAARAANVHVAGWVGDRA
                     TLARMIASADAILHCSSAETYGFAVAEALCCGTPAIVPNAGGAGDLAAADYAQTYPPG
                     DADAAAKAILDLLARDRANLSSAALTAGQQRIGDIESHFEKLFALYARAVQGKRTQLS
                     ANNNRMMEPHASV"
     gene            complement(6277..>6705)
                     /locus_tag="VGC27_03250"
     CDS             complement(6277..>6705)
                     /locus_tag="VGC27_03250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0281623.1"
                     /translation="VLIVGDGPERTALERQLPRAVFTGFLVGDELARAYASSDAFVFP
                     SETETFGNVTLEAMASGLPCVCADATGSRSLVVHGRTGYLAKPRQAEEFADDIATLAL
                     DPEARLKMGAAARERSLGFSWDESMARILGYYKSLAGAPA"
BASE COUNT         1128 a         2137 c         2175 g         1265 t
ORIGIN      
        1 gccgcagcaa tcgaaggcga ccgccgggcg ctgttgagcg gccgcctcca tcaacgaggc
       61 cggcaaccct tccgcgcgcg agcagttgat gacggcgtca gccgccgcga ccaccgggcg
      121 ctgatcggat acgaacccga ggaggcgtac gcggtcttca acgccgcgtt gtttgacaag
      181 tgattcgagc cgtgcgcgtt cgctgccctc ccccacgatg tcgagccgga cgccgggcgt
      241 ttttgccacc gcttcgatcg ccacggagac ctgcttgacc ggttcgaggc gacagacgat
      301 ggcaaaggtg aacggcccct cgggtggagg cggcgcgggc gtgaaccatg tcgtgtcgat
      361 gccgaccagg atgacttgga tatggtcgcg agcatgcggc gcaatggaag cggccgcttt
      421 cgccacatgc gcgctcaccg cggcgacccg ggtcgtgtgg cgcaaggccc aatggcggaa
      481 cagcgcacag gacgggtctt ggtagtggcg gacattgtgc tctgtacgaa gcaccggcag
      541 ggcgtatcgc agcccggtgc gagcggcgag aacgagcgcc ccatgggtgt gggtgtgaag
      601 cagatcggcg ccttcttcct tcagcacacg cccgagccac gcaatatcaa gcggccccca
      661 ggtgcgccac agataggcga ggggattctc ccggactggt ccccgatcgt agatgcggac
      721 cccggcgtcc gtgaaaaaag cgcgcagttt cggattgggc gtcatcaacg cgatgctatg
      781 gtcggcgccg gacaattcgg gccgggtcgc gagattgaca aggaagcgtt cggcgccgcc
      841 gatctctccg gcaacgacga cgtgaacgac tttcgggcgg gagcgtggct gcatgttgct
      901 tgtctggttg agcggccttg gcaacgcgaa cgtggcgaac cgcctttgaa tgcagtgtgg
      961 gatatttcgc gggtgaccgc cagggccggc acggcggcaa gcggcgatta accgttcgtt
     1021 gcggctccgg ggccgcggcg ccgcgcgggc ctgtacacgg attggaccct gcctatatca
     1081 tccgcgccac cgacaggggg ccgacaagaa ttggggcgga ttcgaagagt cgatttggat
     1141 cgtccttcaa cggaaacgta aagaggcatg ctgtccgtac aacatctggt gaaatcgctg
     1201 gccgggcgcc gggtgattga agatgtgacg ctgtcgcttg agcgcggcga aatcgtcggc
     1261 ttgctcgggc cgaacggagc gggcaagacg accaccttcc ggatgctgac cggccttgtc
     1321 atcgcggatg cgggcaggat cgagcttgac ggcgccgata tcaccaacct gccgttctac
     1381 gaacgcgccc ggcgcggcgt cagctatctg ccccaggaat ccttcgtgcc gcgctcgctt
     1441 tcggtcgagg acaacattgc gatggtcctg gaagtccgcg agcgttctgc gaaaaaacgc
     1501 agcgagaaac tcgataccct gatctcccag tttcatctcg atgagcttcg cagggtaccc
     1561 gtcggtacgc tctccagcgg tcagcggcgc cgctgcgaga ttgcgatcac gctcgcctgc
     1621 gaaccgggct tcgcgctcct cgacgagccg tttgcgcgcg tcgatcccaa tgcgatcgag
     1681 gaaatcacgg tgctcatccg tcatttgacc gatcgcggca tcggcgtgct catcaccgat
     1741 cacaatgcgc gcgagctgtt tcagctggtc gatcgcgctt acgtcatcga gaacggccgc
     1801 gtgctcgcgc acggcagcgc cgaagaactc atcgccgacc tcaacgttcg ccaagcctat
     1861 ctcggtgagg cgtttcggat atgatgtgga aaccggccgc gtggattgcg aaccttcacc
     1921 gccgcatcct gaaggcgtgg ctgcggaccg tttgcgctcc gttcttgcat ctctacgcgc
     1981 ggaatttttt cgccggcgtg gcgctgctgg tgttcaccat catggccctc gtcgaagcgg
     2041 tctttctggc cgagcggttc cccatggtgt tccgggaggt gttcaggcac cacgccgatc
     2101 cgctcgatac gactttgctg ttcctgtgca acagtacgca agtcttcgat ctggctctcg
     2161 cgatcgcgat cctcatggcg gtgtattgga caacgctgcg gatgcgcgag aatcgcgaac
     2221 tgctggttct cttcgcggcg ggaacggggc cgtatcggct cctggctctc gtcctggtca
     2281 tcgccgtcgc cgcccaaatc ggctcgttga cgatttcggg cgtgctcgat ccggcggccc
     2341 gttatgccca gcgtgtgatc ctgttcaacg ccgagtttcg cgccctcaga agcggaatca
     2401 acaccggaca gttctacagt tttcccggcc gggtcgcgtt tgcccccgcc aggccggcgg
     2461 agcgcgatcg cgcggagccc gagcgcacca gggggctgtt cgtttacgag gaccttaagc
     2521 ccggcaaatt ccgggtcatc acggccgatc acgcgcgtct tgacgggccg gacgcatccg
     2581 gcaaggtcgt gctgaggctg ggcgggttga cggcgctcac cttctcggac gagccgcccg
     2641 cggcaggcgc cgggcccgca ccggcaaaga ccggtgcggt tgcatgcacg gactgccccg
     2701 tacaaccgag cggcgtgcct cgcatgacgc tttccgcgag cgacgttgtt caagagatgg
     2761 cgagcgatca gctgctcacc ttcatgccgc gaggaagcaa gagcgaagag atgacgattt
     2821 tcgagcagtt cgccgcaacg tccgattcct cctcgctgag gcaccgggag gacatgcgcc
     2881 tgctcggcgg gcggcttgcg cgcagctttc tgtgtctgct tgcgccgctt gttgccctgg
     2941 cttgcatttg cttcacgacg cgcgccaccc attatttcgc cttgccgatc gcctgcatgg
     3001 gcctgatgtc gctcaacgtg acgagtgaat ggctcgtcag agtgaccgtg ccattgaacc
     3061 ctctcggcgc cttgagcgtg ccggcggcac tgacggcggt gttcgcggcc ttgctgctca
     3121 ccgaaatctt ccgtgagcaa ggcatgctgg cgcggccgca actggcgcgc tcatgagcat
     3181 caacacgctt tccggcccgg aaaaaagagc gccgcgggcg atccgttttt cgcccatggg
     3241 acggcacaca agatacatgc tggcggcgta tttgcgtaac gtgttcatgg tgatctcggt
     3301 tctgctcgcc atcgcgctga cgatcgattt gtggccgcag tttcaaatca ttgccggcga
     3361 tcagggcgat ggcgcgctgg ccgcaacctg gagggttctg cgcttttcgg cattgcgcac
     3421 gcccggcctt gtcgcgccgt tccttccgtt tgcgacgttc ctgggcgtcg tctggaccga
     3481 ggtggtgcac actcaatccg gcgagcgcat gctggtgtgg aacagcggcc gctcgccgat
     3541 ccaatgcctg gcgcccgcat tcctgctggg tgtcattctc ggcgccgggg aattcgcgat
     3601 ggacgcctat ctcggcccgg ccgcgatgtc cgtgcagatg catgaacggc tggggcttga
     3661 cggccagcgg ctggatcgta cccgcatcag aagcgcctcc tggattgcat tgcccgacgg
     3721 actcctcaga gcggaaatcg aatacgggcc tcctgcggtt ctgcatcgcc tgacgtcttt
     3781 taaacgcgat gccgccggcc agttgaccga agtcgacatg gcggccgtcg cacgccggca
     3841 acccggcacg aatctctggc tgctgcacga tggccgttct tggtcggcga agggagcgac
     3901 ggcgcaggat gacgcgtcgc tcccggttgt cgcgctcggc gggttcacga acgaaacgat
     3961 ggttcccttc aagacgcgga cgatctcgct ggatctcgat ccgctttggc tcagcgtgtt
     4021 cggtatggaa acacagtacc ttcccctgca cgtcctgcga cagttggttc ggtccgaccg
     4081 ggactcggat tccagaacct cctatcgcac acgcctgcaa gtgctgtacg gcgaagcttt
     4141 tcttccgggc gcaatggccc tcctggccgc atcgctttcg ctgcttcttc tggcctacgc
     4201 tacgcgggcg acggccctcg tcggcatcgt ctttgtcggc tacatggcgc atttcggcac
     4261 caaagcgtgc ctgctgatgg gccaaaccgg cttcctgccg cccgcggtgg ccggctggtt
     4321 cgtgccggcc ggtcttttgg tggcgacctt ggcggttctt ggcgtcatcg agcggcagcg
     4381 ccgccgaatc tgagccggga ggagagatcc cgcgccgcgt tatttgtgcg gaacaacgtg
     4441 ctcgcgcagg ggcttgccgg tcagcttgtc gtagccgaag aagtccaagg ccgtgacgtc
     4501 cggcagcaac ccgctgtcgt cgacctcaag atagacatag accagcttta cccagcgatt
     4561 gccgactttc ccgaacgctt ccggctgccc cttgccgtcc agaccgacat agagcgtccg
     4621 ttcaggaagc gcggcgatct tgaaagtgaa ggtcccgtcc gccttgttgc gcttcacgga
     4681 tttgacgccg taggccatca tgcgttccgc cagattgagc gctttgcgat ggcccccgcg
     4741 attgtaccag cgccaatagg caatcaccgg ctcgtccgca acgatgtgcc cctgcgcgtc
     4801 gagattcgcc gcatacacca ccgtgttgga gttgaccgag cgctcgatgt agaaaagcat
     4861 gttcggttcg ttcggcaccg gaaactcggg ccgcacgcgc gggatttgct cgagttccgt
     4921 gacttggctg gtcaactcgc gcccctcggc tccggccgcc gaaaccatca ggcaaaatgc
     4981 cgccgccacg ccggcaagaa ggcgacgtcc tgtcgccgct cgcttcgcgc cggtctttct
     5041 tcgggccggc aattgggaga gatcaaacac tcgcatgggg ttccatcatc cgattgttgt
     5101 ttgccgacaa ttgcgtgcgc ttgccctgga cggcccgcgc ataaagggca aagagctttt
     5161 cgaagtggct ttcgatgtcg ccgatgcgct gctgtccggc ggtgagcgcg gcgctcgaaa
     5221 ggttcgcgcg atcgcgtgcc aacaggtcga ggatggcttt ggcggccgca tccgcgtcgc
     5281 ccggcggata ggtttgggca tagtccgcag ccgccagatc gcccgcgccg cccgcgttcg
     5341 gcacgatggc gggggtgccg cagcacagcg cttcggcgac ggcaaagcca taggtctcgg
     5401 cgctcgaaca gtgaagaatg gcgtcggcgc tcgcgatcat gcgggcaagc gtcgcgcggt
     5461 cgcccaccca accggcgacg tgaacatttg cggcgcgcgc cgccttggcc tcgatgctcg
     5521 tatggatgaa gccgtcgccg atgacataaa ggccgacggg cctggatttc tgggccttgg
     5581 tgaccgcgtc gatggcggtg ccgacgcgct tttccggatg atggcgtccg atattgacca
     5641 gcagcgccgc gccgggtcca aggccgcatt ttgcaagcat ggccgtgcgc agcgtcaggt
     5701 cgcgaaggcc ggggccgaac catttggttt cgacgccgaa cggaacgacg tgcagattgc
     5761 gcaagccgtg gccggcgaag cgcgtcgcca gccaggagcc ggcgacgatg cagccgtcga
     5821 agcgcgcgtt cagccgccgc agatacgacc agtaccaagc gaagagcgtg tcgatcccgg
     5881 aaggcgacag cagcccggag agcagcatct gcggatacac ggcaaccggg tcggcatgca
     5941 tgaacagcat cttcacggcc gggcctcgcc attgcccggc gatccagccg ccgcgccaag
     6001 gcgaggaacc ttcgaccaca tcgggcgtct cgccgtcgac gatccgcaga acctccgccg
     6061 cgcgccagaa catgcggtag ttgcggtcga acggcagttg aggcgtctcg acccatacca
     6121 gcttgccgcc ctcgcgcgcc tcgacgcgac tttccgtgcc gggagcgatc acggtcaagc
     6181 aatggccgtg ccgcgctgcc gctttgaact tgtgatcgac gtaattgcgc acgccgccgc
     6241 ccgtcggcga gtagaactcg gagatgtcga cgatcttcat gcgggcgctc ccgcgagaga
     6301 tttgtaatag ccgagaatgc gcgccatcga ttcgtcccag gaaaaaccga gactgcgttc
     6361 gcgcgcggcg gctcccatct tcaggcgtgc ctccgggtcg agcgccagcg tcgcgatgtc
     6421 gtccgcgaat tcctctgcct gcctcggctt ggcgagatag ccggtgcggc catgcacgac
     6481 cagagagcgg ctgcccgtgg catcggcgca aacgcatggc aggcccgacg ccatggcttc
     6541 gagcgtcaca ttgccgaagg tctccgtctc gctgggaaac acgaaggcgt cggacgaggc
     6601 gtaagcccgg gcgagttcgt ctcccaccag aaagccggtg aagacggcgc gcggcaattg
     6661 tctttccaag gcggtgcgtt cgggaccgtc gcccacgatc aggac
//
LOCUS       DASWPU010000143         3128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_56167,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000143 DASWPU010000000
VERSION     DASWPU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56167"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1363)
                     /gene="hemA"
                     /locus_tag="VGC27_03255"
     CDS             complement(146..1363)
                     /gene="hemA"
                     /locus_tag="VGC27_03255"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEY0281624.1"
                     /translation="MAFSYLSRFQDALAGLKREGRYRVFADLVRRRGDFPAADLFVEG
                     VKRPVTVWCSNDYLGMGQHPEVIAAMHEAIASAGAGAGGTRNISGTTHYHVELERELA
                     DLHRKEAALLFSSGYVSNDTTLATLAKLLPGVVLLSDELNHASMIEGIRHSGAEKRIF
                     RHNDLAHLEELLRTVDPALPKIIAFESVYSMDGDFGPTAAVCDLAEKYGALTYLDEVH
                     GVGLYGPRGAGVAARDGSMHRIDIIEGTLAKAFGLMGGYITGKAEVIDCVRSFAPGFI
                     FTSSLAPAIAAGALASIRHLKVSDAERKAIHENAARLKSLLRNAGLPVINSPSHIVPV
                     MVGDAALCKSVADALLHRHAIYVQPVNYPTVPRGKERLRFTPSPLHSNKMMDTLVQAL
                     DEVWTDHHLQRAA"
     gene            complement(1502..2149)
                     /locus_tag="VGC27_03260"
     CDS             complement(1502..2149)
                     /locus_tag="VGC27_03260"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY0281625.1"
                     /translation="MKHKLDGHLRHHGRFYVSALLGAAVFAAMSLANVSDAAAAAGDT
                     FFAVYLAASAWLVRKSTKADVKRRAKTEDEGAMIVIALALATVAFSVAGIFVVLNQAH
                     RPDLLSLMLALAAAPLGWCMLHTISAFHYANLFYFTSASEAPGKALEFPGTEEPDLWD
                     FVYFSFVIGMTAQVSDVQIRTTGMRRTVTGHSVVSFFFNTVLIAMAVNAAVASAG"
     gene            complement(2211..2720)
                     /locus_tag="VGC27_03265"
     CDS             complement(2211..2720)
                     /locus_tag="VGC27_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEY0281626.1"
                     /translation="MTALTRPQESAAIETARTQGKVYLETLNLLERLHRQLLDVIKDE
                     LDRREEREINSVQALLLFNVGDQELSAGELRSRGHYLGSNVSYNLKKLVDLGYIHHER
                     SEVDRRSVLVRLTQKGEAVRDMLRELFERHLGSLEAVGGVSTPDLESLNGTLKRLERF
                     WIDQVRFRL"
     gene            complement(2920..>3128)
                     /locus_tag="VGC27_03270"
     CDS             complement(2920..>3128)
                     /locus_tag="VGC27_03270"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0281627.1"
                     /translation="TLSFDDCMRMEFRIASRILAGHDFYEGVRAVLVDKDNAPRWRPA
                     SLGEVTDDTVGAYFMPLGANELPL"
BASE COUNT          594 a          989 c          991 g          554 t
ORIGIN      
        1 tccgatggtc gcctcgaatt acgccgcgca acgctccgaa ttcgcgaaaa agatcggcct
       61 cggccgcagc gcaccggcaa cgaagagccg caagcgcgga taattcatcg gcgaacagcg
      121 aatcgggatc gccgttcgcc ccttatcacg ccgcgcgctg gaggtgatgg tccgtccata
      181 cctcgtcgag cgcttgcacc agcgtatcca tcatcttgtt gctgtgcagg ggcgaaggcg
      241 tgaaacgcag acgttccttg ccgcgcggga cggtcgggta gttgaccggc tggacgtaga
      301 tcgcgtgccg gtgcagaagc gcgtccgcca cggacttgca cagcgcggcg tctcccacca
      361 tcaccggcac gatgtggctc ggcgagttga tcaccggcag tccggcgttg cgcaacagcg
      421 acttcagccg cgccgcattc tcgtggatcg ccttgcgctc ggcgtcgctg accttgaggt
      481 ggcggatgct ggcgagcgct cccgccgcga tggccggcgc cagcgaactg gtgaagatga
      541 accccggcgc aaacgagcgc acgcaatcga tcacttccgc cttgccggtg atatagccgc
      601 ccatcaggcc gaaggccttg gccagggtgc cttcgatgat atcgatgcgg tgcatggagc
      661 cgtcgcgcgc cgcgacgccc gccccgcgcg ggccgtagag cccgacgcca tgcacttcgt
      721 cgagataggt cagcgcgccg tatttttccg cgaggtcgca gaccgcggcg gtcggcccga
      781 aatcgccgtc catcgaataa acggattcga acgcgatgat cttcggcaag gcgggatcga
      841 ccgtccgcag cagctcttcg agatgggcca gatcgttgtg gcggaaaatc cgtttctcgg
      901 cgccgctgtg gcggatgcct tcgatcatcg aggcgtggtt gagttcgtcg gacaagagca
      961 cgacgcccgg caacagcttt gccagggtcg ccaaggtcgt atcgttggag acatagcccg
     1021 aactgaacag caaggccgct tccttgcgat gcagatcggc cagttcccgt tcgagttcga
     1081 catggtaatg cgtggtgccg gaaatgttgc gggtgccgcc ggcgccggcg ccggcgctgg
     1141 cgatcgcttc gtgcatggcg gctatgacct cgggatgctg gcccatgccg agatagtcgt
     1201 tgctgcacca gacggtcacc gggcgcttga cgccctctac aaacagatcg gcggccggga
     1261 agtccccgcg cctgcgcacg agatcggcaa acacccggta acgcccctcg cgcttcagcc
     1321 cggccaaagc gtcctggaac cgggaaagat aggaaaatgc catgtttttg ctcttacaac
     1381 tccgcggaaa ttcttgcttg gattgtccac catgtccgcc ggacagttca acccggcgga
     1441 ccccgttcat acgatttgtc acacagcggc tagggatgat agcgctgccg gccggaaaag
     1501 gctaacccgc actggcaacg gcggcattta ccgccatggc gatcagcacc gtgttgaaga
     1561 agaacgacac gaccgagtgg cccgtcaccg tccggcgcat cccggtggtg cgtatctgca
     1621 cgtccgaaac ctgggcggtc atcccgatca cgaacgagaa atagacgaaa tcccacaaat
     1681 ccggctcttc cgtgccggga aattccaggg ccttgcccgg agcttccgat gcggaagtga
     1741 agtaaaacag gttggcgtag tgaaaggccg agatcgtgtg cagcatgcac cagccgagcg
     1801 gcgcggcggc cagagccagc atcagcgaaa gcaggtcggg ccggtgcgcc tgattgagca
     1861 cgacgaatat gcccgcgacg ctgaacgcca cggtcgcgag cgccagcgca atgacgatca
     1921 tcgcgccttc gtcttccgtc ttggcgcggc gtttcacgtc ggccttggtc gatttgcgga
     1981 ccagccaggc gctggcggcg agatagacgg cgaaaaacgt gtcgcctgcc gccgcggcgg
     2041 catcggaaac gttcgccaag ctcatcgccg cgaaaacggc cgcgccgagc aaggcggaaa
     2101 cgtaaaagcg cccgtgatgg cgaagatgtc cgtcgagctt atgcttcaag gcgtgcgtcc
     2161 ggccgccggc tgccccgcca tcatggcacg agtcggggtg ccgccgggac ctagagccgg
     2221 aaacgaacct ggtcgatcca gaagcgctcc agccgcttca gcgtgccgtt caggctttcc
     2281 agatccgggg tggagacgcc gcccacggcc tccagcgaac ccagatggcg ctcgaatagt
     2341 tcgcgcagca tgtcgcgcac ggcctcgccc ttctgcgtca ggcgcaccag caccgagcgg
     2401 cggtccactt ccgaacgttc gtggtggatg tagccgagat cgacgagctt cttcagattg
     2461 taggagacgt tggagccgag gtaatggccg cgcgagcgca actcgcccgc gctcagttcc
     2521 tggtcgccga cgttgaacag gagcagcgcc tgaacactgt tgatctcgcg ttcttcgcgc
     2581 cggtcgagtt cgtccttgat cacgtcgaga agctggcgat gcagccgctc gagaaggttc
     2641 agcgtctcga gatagacctt gccctgcgta cgtgcggtct cgatcgccgc agattcctgc
     2701 ggtcttgtca gtgccgtcat cgtcttcccc cctttgcggg ggcccacctg ttgtttgcgg
     2761 aaagcctatg ccgcaggtct taagggtgtg taaaaactgc ctagaataag ctgcaaaatc
     2821 gcgataaacg gcgcgcgacg ggcggatcgc cgcagttcgc ggcaaataat tcgttaagct
     2881 gttccgcctc tgcactaatc gccatggttg caggcgccct cacaagggca gctcgtttgc
     2941 ccccaaaggc atgaagtagg cgccgaccgt atcgtccgtc acctcgccaa gagacgccgg
     3001 ccgccagcgt ggcgcgttgt ccttgtcgac aagcaccgcg cgtacgccct cgtaaaagtc
     3061 gtgcccggcg aggatgcggc tggcgatgcg gaactccatg cgcatgcaat cgtcgaagga
     3121 gagcgtct
//
LOCUS       DASWPU010000144         3889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_38016,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000144 DASWPU010000000
VERSION     DASWPU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38016"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VGC27_03275"
     CDS             <1..1133
                     /locus_tag="VGC27_03275"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEY0281628.1"
                     /translation="FGKEEPGDGESYGRFTTGILPGHVLKRLIRAHREIVATEDVDDV
                     QIQPASIDLRLGPVAWRVRASFLPGPHATVRDKLVNAVLHEIDLTDGAVLETGCVYVV
                     PLLESAEFSFRVSGIANPKSSTGRLDVFTRLITDRAQGFDRIEPGYHGPLYAEISPRT
                     FPVLVRKGSRLNQLRVRKGSPQFTDTQLRRLHEETPLVDGEADIDNGLALSVDLKGDA
                     AVHHVGWRAKRHTGIIDIDKRDVLDPLDYWDPIQASKTGTIVLDPDEFYILASREAVA
                     IPSEYAAEMVPFNPLVGEFRVHYAGFFDPGFGYQPGKPPCARAVLEVRSREVPFILEH
                     GQIIGRLVYERLTEVPSEVYGEGLGSHYQRQGLKLSKHFKPI"
     gene            complement(1352..>3889)
                     /gene="metH"
                     /locus_tag="VGC27_03280"
     CDS             complement(1352..>3889)
                     /gene="metH"
                     /locus_tag="VGC27_03280"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY0281629.1"
                     /translation="QVANGAQIIDVNMDEGLIDSEAAMRRFLNLIAGEPDIAKVPLMI
                     DSSKWSVIEAGLKCVQGKAIVNSISLKEGEAAFVARAREVRRYGAAVVVMAFDEEGQA
                     DTRERKFNICKRAYDILVNQVGFPPEDIIFDPNIFAVATGIEEHNEYGVAFIEAAGRI
                     RAELPHVHISGGVSNFSFSFRGNDTVREAMHSVFLFHAIKAGMDMGIVNAGQLTVYQN
                     IPTPLREGIEDVLFNRRADATERLLDLAGQFKGGGAVAEVEDAAWRALPVEERITHAL
                     VHGIDAFVVEDTEAARLKAARPLDVIEGPLMAGMNMVGDLFGSGKMFLPQVVKSARVM
                     KKAVAHLLPFMEAEQAKNQAKEPVGRIIMATVKGDVHDIGKNIVGVVLQCNGYEVIDL
                     GVMTPAQKILDTAREKGANIIGLSGLITPSLDEMCHVAAEMERQGFDIPLLIGGATTS
                     RVHTAVKIDPNYRRGQAVYVTDASRAVGVASSLLSKDANAGYVAGVRREYEEIARNHA
                     AQRAPGRRLSLQAARANKLKFDWANYAPPVPAFLGVRSLDNYDLAELVPYIDWTPFFQ
                     TWELVGKYPAILEDGKYGFAARGLFDDAQKMLAKIVGERWLTARAAFGFFPANAQDDD
                     IFVFDGEARETSIATLHTLRQQMSRDSGKPNLALADFIAPKGVADYIGGFAVTAGHGE
                     EAHVKRFEADKDDYSAILLRALADRLAEAFAERLHERVRREFWAYAPDENLSGDQLIA
                     EAYRGIRPAPGYPAQPEHSEKGTLFELLDVEARAGVKLTESFAMWPGSSVSGFYFSHP
                     QSRYFGVGKIEKDQVEDYARRKGWTLEEAKRWLAPILNYDPRTVAAA"
BASE COUNT          675 a         1258 c         1236 g          720 t
ORIGIN      
        1 tgttcggcaa ggaagaaccc ggtgacggcg aaagctacgg ccgcttcacc accggcatcc
       61 tgcccggcca tgtgctgaag cgcctgatcc gcgcccaccg cgaaatcgtg gccaccgaag
      121 atgtggacga cgtgcaaatt cagccggcga gcatcgatct caggctcggc ccggtggcct
      181 ggcgggtgcg cgcgagtttc cttcccggcc cccacgcgac ggtgcgcgac aagctcgtca
      241 atgccgtctt gcacgagatc gatctgacgg acggcgccgt gctggagacg ggctgcgtct
      301 atgtcgtgcc gctcctggaa agcgcggagt tttccttccg cgtctccggc atcgccaatc
      361 cgaaaagctc gaccggacgg ctcgacgtct tcacccgcct gatcaccgac cgcgcccaag
      421 gcttcgaccg catcgagccg ggctatcacg ggccgctcta cgccgagatt tcgccgcgta
      481 cttttccggt cctggtgcgc aaaggctcgc gcctgaacca gcttcgcgtg cgcaaaggct
      541 cgccgcaatt caccgacacg cagctgaggc gcctgcacga agaaaccccg ctggtggacg
      601 gcgaggccga catcgacaac ggcttggcgc tgagcgtgga tttaaaaggc gacgcggccg
      661 tccatcacgt cggctggcgg gccaagcggc ataccggcat catcgacatc gacaagcgcg
      721 acgttctcga cccgctggat tattgggacc ccatccaggc cagcaagacg ggaaccatcg
      781 tgctcgaccc cgacgagttc tacatcctgg cgtcgcgcga agcggtggcg atcccgtccg
      841 aatacgcggc cgagatggtg ccgttcaatc cgctggtggg cgagttcagg gtgcattatg
      901 cgggcttctt cgaccccggt ttcggctatc agcccggcaa gccgccttgc gcgcgcgcgg
      961 tgctcgaagt gcgctcgcgc gaagtgcctt tcattctgga gcacggacaa atcatcggcc
     1021 ggctggtgta cgagcggctg accgaagtgc cgtcggaggt ttatggcgaa gggctcggct
     1081 cccattacca gcgccaaggt ttgaaacttt ctaaacattt caagcctatt tgaccgttaa
     1141 ccctgcctaa ttgttgcttt aaatccacat gctcgccaaa aaatcctggc cgatgacaac
     1201 ggaccgttgc gtcgccccgg caaacttgag ctacttgcgc ccgctgcgga ttcaaacggc
     1261 aggggcaaag gtccgcagtt actgggtccg cgttgaaggg ggcagaatga ggcggctacg
     1321 gcgctaaagg cgccggccgc ctcatcgttt ttcaggccgc cgccaccgtg cgcggatcat
     1381 agttgaggat cggcgccagc cagcgtttgg cctcttcgag cgtccaaccc ttgcggcggg
     1441 cgtaatcctc cacctgatcc ttttcgatct tgccgacgcc gaaatagcgg ctttggggat
     1501 gggagaaata gaaccccgac accgacgatc ccggccacat cgcgaagctc tcggtgagct
     1561 tgacgccggc gcgcgcctcg acatcgagca gttcgaatag cgtgcccttc tcgctgtgct
     1621 cgggctgggc gggatagccg ggcgcggggc ggataccgcg ataggcttcg gcgataagct
     1681 gatcgccgct gagattttcg tccggcgcat aggcccagaa ttcgcggcgc acccgctcgt
     1741 gcaggcgctc ggcgaaggct tcggccaaac ggtcggccag cgcacgcagc agaatggcgg
     1801 agtaatcgtc cttgtcggcc tcgaagcgct tgacatgcgc ctcttcgccg tggccggcgg
     1861 tgacggcaaa gccgccgata taatcggcca cgcccttggg cgcgatgaaa tcggccagcg
     1921 ccagattggg cttgcccgaa tcccgcgaca tctgctggcg cagcgtgtgc agcgtggcga
     1981 tggacgtttc gcgcgcctcg ccgtcgaaga cgaaaatgtc gtcgtcctgc gcgtttgccg
     2041 ggaagaaacc gaacgcggcg cgcgccgtca gccatctctc gccgacgatc ttcgccagca
     2101 tcttctgcgc atcatcgaac aacccgcgcg cggcgaaacc gtatttgccg tcttcgagaa
     2161 tggcggggta tttgccgacc agttcccagg tctggaagaa cggcgtccag tcgatatagg
     2221 gcaccaactc ggcgaggtcg taattgtcga ggctgcgcac acccaggaac gcaggaaccg
     2281 gcggcgcgta attggcccag tcgaatttca gcttgttggc gcgggcggcc tggagcgaga
     2341 ggcggcggcc cggcgcgcgc tgggccgcgt gattgcgggc gatctcttca tattcgcggc
     2401 ggacgcccgc gacatatccg gcgttggcgt ccttgctcag caggctcgag gccacgccca
     2461 ccgcgcgcga ggcatcggtg acatagaccg cctgcccgcg gcggtagttc gggtcgatct
     2521 tcaccgccgt atgcacgcgg ctggtagtcg ctccgccgat gagcagcgga atatcgaaac
     2581 cctggcgctc catctcggcg gcgacgtggc acatctcgtc gagcgacggc gtgatcaggc
     2641 cggacaggcc gatgatgttg gcgccctttt cgcgcgcggt gtcgaggatc ttctgcgccg
     2701 gagtcatcac gcccaggtcg atcacttcgt agccgttgca ctggagcacg acgccgacga
     2761 tgttcttgcc gatatcgtgc acgtcgccct tcaccgtcgc catgatgata cggcccacag
     2821 gttccttggc ctggttcttc gcctgctcgg cctccatgaa aggcagcaga tgtgcgacgg
     2881 ccttcttcat cacacgggcc gatttcacca cctgcggcag gaacatcttg cccgaaccga
     2941 acaggtcgcc gaccatgttc atgccggcca tcagcggacc ttcgatcacg tcgagcgggc
     3001 gcgccgcctt gagccgcgcc gcttcggtgt cttccaccac gaaggcgtcg atgccgtgga
     3061 ccagggcgtg agtgatgcgc tcctccaccg gcagggcgcg ccaagcggcg tcttcgactt
     3121 ccgccaccgc gccgccgccc ttgaactgtc cggcaaggtc gagcagtcgc tcggtggcgt
     3181 cggcgcggcg gttgaacagc acgtcctcga tgccctcgcg cagcggcgtg gggatgttct
     3241 gatagacggt aagctgcccg gcattgacga tgcccatgtc catgccggcc ttgatggcgt
     3301 ggaacagaaa cacggaatgc atcgcctcgc gcaccgtatc gttgccgcgg aacgagaagg
     3361 agaagttcga caccccgccg gaaatgtgga cgtgcggcag ctcggcgcgg atgcgccccg
     3421 ccgcctcgat gaaggcgacg ccgtattcgt tgtgctcctc aatgccggtg gcgaccgcga
     3481 agatgttggg gtcgaagatg atgtcttccg gcgggaagcc gacctggttc accaggatat
     3541 cgtaggcgcg tttgcagata ttgaacttgc gttcgcgcgt atcggcctgg ccctcttcat
     3601 cgaaggccat gaccaccacg gccgcgccgt agcggcggac ttccctggcg cgggcgacga
     3661 acgccgcctc gccctctttg agcgagatcg aattgacgat ggccttgcct tgcacgcatt
     3721 tcagcccggc ttcgatcacg ctccacttcg agctgtcgat catcagcggc actttggcga
     3781 tgtcgggttc gcctgcgatc aggttgagga agcggcgcat cgcggcctcg gaatcgatca
     3841 ggccctcgtc catgttgacg tcgatgatct gggcgccgtt ggcgacctg
//
LOCUS       DASWPU010000145         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_56168,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000145 DASWPU010000000
VERSION     DASWPU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56168"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VGC27_03285"
     CDS             <1..452
                     /locus_tag="VGC27_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY0281630.1"
                     /translation="IDILADNCAMQLVRAPKTLDVILTDNLFGDILSDEAAQLTGSIG
                     MLPSASLGAKNKDGRQSALYEPIHGSAPDIAGQGLANPIAAILSFAMCLRYSFGRGED
                     AALLEKAVERVLAEGYRTGDIMQSGMTRVGTSAMTDVILKTLYRLAN"
     gene            complement(550..1224)
                     /locus_tag="VGC27_03290"
     CDS             complement(550..1224)
                     /locus_tag="VGC27_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0281631.1"
                     /translation="MDTTSLKNPPRRAASHGKRERTKAQNRQTILDAARQVFAELGYG
                     ATTVRDIIRATPLASGTFYNYFTSKEEVYQAIRDEVALSIRPRLHEERMKATTVEEFI
                     TGSFRTFFEFVAGDAMNLRAIRHSADATRVRMDTPEVIAGFDELRGDIEMAVAKGLFP
                     PVDADYLMAAIVGVAFEVAERMLQREEHDTAAAAAFAAALFIGGVRNLPCAAGSDVKE
                     IVQPSL"
     gene            complement(1384..>1769)
                     /locus_tag="VGC27_03295"
     CDS             complement(1384..>1769)
                     /locus_tag="VGC27_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0281632.1"
                     /translation="LGKLLLRVGLGGLLLFHGVYKLLNGLEPIKTMLASHNIPDALAY
                     GVYLGELVAPVLLIVGQFSRLGGLLVAFNMIVALLLAHTASLIALTPETGGYALELQA
                     FYLIGGLAVALLGAGRFSLGNRIWN"
BASE COUNT          344 a          556 c          564 g          305 t
ORIGIN      
        1 tgatcgacat cctggccgac aactgcgcca tgcaattggt gcgagcaccg aaaaccctcg
       61 acgtcatcct gaccgacaat ctgttcggcg acattctgtc ggacgaagcg gcgcagctca
      121 ccggctcgat cggcatgctg ccgtcggcct cgctgggcgc caagaacaag gacggccgcc
      181 agagcgcgct ctacgaaccg atccacggca gcgcgccaga catcgcgggc caaggactcg
      241 ccaatccgat cgccgcgatc ctgtccttcg ccatgtgcct gcgctattcg ttcgggcgcg
      301 gcgaggacgc ggcgctgctg gagaaggcgg tggagcgcgt gctggcggaa ggctatcgca
      361 ccggcgacat catgcagtcc ggcatgaccc gggtcggcac ctccgcgatg accgacgtca
      421 tcctgaagac gctgtacagg ttggcgaatt aaagctttca aagaatcttt tcaccctccc
      481 tcaacccgtc ttcgctgaag ctccgacggg ttgggcgcgc ggtccggcga agccttggcg
      541 tagccgggct caaagggatg gttggactat ttccttgacg tccgatccgg ccgcgcaggg
      601 cagattgcgc acaccgccga tgaacagcgc ggccgcgaac gccgcggcgg cggcggtatc
      661 gtgctcctcg cgctgcaaca tgcgttcggc cacctcgaag gcgacgccga cgatcgcggc
      721 catcaggtaa tcggcatcga ccggaggaaa caggcccttg gcgacagcca tttcgatatc
      781 gccgcgcagt tcgtcgaagc cggcaatgac ctccggcgta tccatgcgca cccgcgtggc
      841 gtcggcggag tggcggatgg cgcgcagatt catcgcgtcg ccggccacga attcgaagaa
      901 ggtgcggaag ctgccggtga tgaactcctc caccgtcgta gccttcatgc gctcttcgtg
      961 gagacgcggc cgaatggaga gcgcaacttc gtcgcggatc gcctggtaca cttcctcttt
     1021 ggacgtgaag tagttgtaga acgtgcccga agccagcggc gtggcgcgga tgatgtcgcg
     1081 caccgtggtc gctccatagc cgagttcggc aaagacctgg cgcgcggcgt ccaggatggt
     1141 ctgacggttc tgggccttcg tccgctcgcg cttgccgtga gacgccgcac gccgcggcgg
     1201 gtttttcagc gaagtcgtgt ccatcgattg ttccggcggt ccaggctgtc gtactttacc
     1261 cgttcgtgac gactcgtcaa ctttcgtcct cgccgatttc gcgcgccgcc gcgaaacgcg
     1321 gccggaatcg ccgcgccttt gcacgggtag aagccatatc cgaggacgga atgaccgaac
     1381 ggatcagttc cagattctgt tgccgagact gaagcgcccc gcgccgagga gcgcgaccgc
     1441 aagaccgccg attagataaa acgcttgcag ctccagcgcg tagccgcccg tctcaggcgt
     1501 caaggcgata agagacgccg tgtgagccag cagcaaagca acgatcatgt tgaaggcgac
     1561 cagcaatccg cccaggcgcg agaactggcc gacgatgagg aggaccggcg caaccagctc
     1621 gccgagatag acgccataag cgagggcatc gggaatattg tgggaggcga gcatcgtctt
     1681 gatcggttcg agaccgttta gaagtttgta gacgccatgg aacagcagca gcccgccaag
     1741 gccgacgcga agcaacaatt tgcctaagt
//
LOCUS       DASWPU010000146         4002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_56169,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000146 DASWPU010000000
VERSION     DASWPU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56169"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..919
                     /locus_tag="VGC27_03300"
     CDS             281..919
                     /locus_tag="VGC27_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281633.1"
                     /translation="MSVVALGRDYPSIFAAPVIRAADTAIFTLRYFTHCMACGFCHDQ
                     CCNYGVDIDLANMERLRSLGPALEAFAGVPQGEWFAAGIVEDAEFPSGAHGRTRAVDG
                     KCVFAGRQERGCKIHAFCLKHGLDYHLYKPLVSILFPLTFEHDVLVPSPEAVDGSLIC
                     AGGGPSLYDGVRGELAHYFGPELIAALDALRERATSRSPSPVPHADGVRARR"
     gene            complement(861..1763)
                     /locus_tag="VGC27_03305"
     CDS             complement(861..1763)
                     /locus_tag="VGC27_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0281634.1"
                     /translation="MRNVSRGIALKIAQTLAFSLMYAAIKLAGNASIGEVVFFRAFFA
                     FVPLLAWTFLTVGPAAAIRTRRPLYHVLRSAVGVTAMFFNFSALKLLTLASVTAFSFM
                     QPIFAVILAALFLGEHVGRFRWGAVIVGFIGVLMMIEPHGGLASLLSLHLSRGVAYAL
                     AFSLFSAAVVVLIRQMSSTERSEAIVFYFMASCTVAGAVAMIWDYVPLTWNMALWLVL
                     CGLVGGAGQIFMTACYRYAEPSLLASFDYISMIWATLLGYFVLAEVPEAMVLAGAGVV
                     IAAGLLIAWREHHLHVERGLENVT"
     gene            complement(1771..2748)
                     /locus_tag="VGC27_03310"
     CDS             complement(1771..2748)
                     /locus_tag="VGC27_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEY0281635.1"
                     /translation="MMKTYLPHNGGLKAAPDAESAPAIAGGLWIDLIDPTPGERQTVN
                     NALHVELPTHADMEEIEISSRLYSEDGGLFMTALILSNSGTEKPVADVVTFILVRDKL
                     ITLRYIDPQPFRTFTARCGRSMIGANKAETALMALIDVIIDRLADILERAGSEIEGIS
                     REIFDSTCGNRPITSHDFRKILRALGRKHDLSSKIRDSLLSLARMLAFLTQAMDTKPN
                     KDVLPHLKTLTRDVQSLQDHSSYMGTKLSYLLDATLGLINIDQNNIIKTMSVAAMVFL
                     PPTLFASIWGMNFHFMPELDEWWGYPLSVVVMVASAVLPYLWFKRKGWL"
     gene            complement(2942..3850)
                     /locus_tag="VGC27_03315"
     CDS             complement(2942..3850)
                     /locus_tag="VGC27_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY0281636.1"
                     /translation="MLKLKEICVALALLLGAMPAAAAPKVLATIKPVHSLTAAVMQGV
                     GEPELLIGGALSEHSYALKPSDARKIERADAIFEIGPDLETYLTAPLAALGAGGRVVV
                     LERAPGVRLLPARHGGLWEDADEHDHGPNDPHLWLDPRNAVAMTEAIAAKLIQLDPAH
                     AAAYRANAAREIAALKALDRELAAKLAPVRDRPYLVFHDAYRYFEARYGLKPAGAVTV
                     APDRPVGPRRIKALRAVLLQGRVACIFREPQFPPKLIGALGEGTSVRIGVLDPLGADL
                     KPGPALYPELLRALAQSLTECLGKNR"
BASE COUNT          735 a         1258 c         1316 g          693 t
ORIGIN      
        1 ctgtttgaga actcctcatg agccgcgttt cgagcgaaaa tagccctatg gcaggagaag
       61 cgcgaagagc gtatcgggta atacggtcga gcgcttcgac gtaccagagg gctattttcg
      121 ccgaaaccct ccgggacgcg ccgattttgc ccgcccggtt cgtcgccggc ctcgtgcgta
      181 ctccccggta cgaccttcgg ccggctcctg ccggatcaga caaaatcggc agcgtcgcgg
      241 ccatggggag ttctcaaaca ggctctaggg cggcgctgcc atgagcgtcg tcgcgctcgg
      301 ccgggactat ccctccattt tcgcggcccc ggtgatccgc gccgcggaca ccgccatctt
      361 cacgctgcgt tacttcacgc actgcatggc ttgcggtttc tgccacgacc aatgctgcaa
      421 ttacggcgtg gacatcgacc ttgccaatat ggagcggctg cgctcgctcg ggcctgcgtt
      481 ggaggcgttc gcgggcgtgc cgcaaggcga atggttcgcg gccggtatcg tcgaggatgc
      541 ggagttccct tccggcgcgc acgggcgcac gcgcgcggtg gacggcaaat gcgttttcgc
      601 cgggcggcag gagcgcggct gcaaaatcca cgccttctgt ctgaagcatg gtctcgacta
      661 tcacctctac aagccgctgg tcagcatcct gttcccgctc accttcgagc atgacgttct
      721 ggtgccctcg ccggaagcgg tggacggcag cctgatctgc gcgggcggcg gccccagcct
      781 ctatgacggg gtgcgcggcg aactcgccca ttacttcggc ccggaactga tcgcggcgct
      841 cgacgcgctg cgcgagcggg ctacgtcacg ttctccaagc cccgttccac atgcagatgg
      901 tgttcgcgcc aggcgatgag caggccggcc gcgatcacca cgcccgcgcc cgccagcacc
      961 atcgcctccg gcacttcggc gagaacgaaa tagcccagca gcgtcgccca gatcatcgag
     1021 atgtaatcga acgacgccag cagcgagggt tcggcatagc ggtagcaggc ggtcatgaag
     1081 atctggccgg cgccgccgac caggccgcac agcaccagcc acagcgccat attccaggtc
     1141 agcgggacgt aatcccagat catggcgacg gcgcccgcca ccgtgcagga ggccatgaaa
     1201 tagaacacga tcgcttcgct gcgttcggtg ctgctcatct ggcggatcag gacgacgacc
     1261 gccgccgaga acagggagaa cgccagcgca taggccacgc cgcgcgacag atgcaggctg
     1321 aggagcgaag ccagcccgcc gtgcggctcg atcatcatta gcacgccgat gaagccgacg
     1381 atcaccgctc cccagcggaa acggccgaca tgctcgccta gaaacagcgc cgccaggatc
     1441 accgcgaaga tcggttgcat gaagctgaac gccgtcacgc tcgccagcgt cagcagcttg
     1501 agggcgctga aattgaagaa catcgcggtc acgccgaccg ccgaacgcaa gacatggtag
     1561 agcgggcgcc gggtgcggat ggcggcggcc ggtcccaccg tcaagaacgt ccaggccagc
     1621 agcggaacga aggcgaagaa agcgcggaaa aacacgacct cgccgatcga ggcgttcccc
     1681 gccagcttga tcgcggcgta catcagcgaa aaggcgagcg tctgcgcgat cttcagcgcg
     1741 atgccgcgcg agacgttcct catcggcgta tcagagccag cctttgcgct tgaaccagag
     1801 gtagggcagt accgccgacg ccaccatcac caccaccgac agcggatagc cccaccattc
     1861 gtccagctcg ggcatgaagt gaaagttcat gccccagatc gaggcgaaca gggtgggggg
     1921 caggaagacc atggcggcga ccgacatggt cttgatgatg ttgttctggt cgatgttgat
     1981 caggcccagc gtcgcgtcga gcagatagct gagcttggtt cccatatagg acgaatggtc
     2041 ctgcagcgac tgcacgtcgc gcgtcagcgt cttgaggtgc ggcagcacgt ccttgttggg
     2101 cttggtgtcc atcgcttggg tcaggaaggc cagcatgcgg gcgagcgaga gcaggctgtc
     2161 gcggatcttg ctgctcagat cgtgcttgcg gcccagggcg cgcaatatct tgcggaaatc
     2221 gtgcgaggtg atcggccggt tgccgcaggt ggaatcgaag atctcgcgcg agatgccttc
     2281 gatctccgag ccggcgcgct ccagaatgtc ggcgaggcgg tcgatgatca cgtcgatcag
     2341 cgccatcagc gcggtctccg ccttgttggc cccgatcatg ctgcgcccgc aacgcgccgt
     2401 gaaggtgcgg aaaggctgcg gatcgatata gcgaagggtg atgagcttgt cgcggacgag
     2461 aatgaacgtc accacgtcgg cgacgggctt ttcggttccg ctgttcgaca ggatcagcgc
     2521 ggtcatgaac agtccgccgt cctcggaata aaggcggctc gagatttcga tctcttccat
     2581 gtcggcgtgg gtcggcagtt ccacgtgcaa ggcgttgttc acggtctggc gctctccggg
     2641 cgtggggtcg atcaagtcga tccacaagcc gcccgcgatc gccggcgcgc tctcggcgtc
     2701 gggcgccgcc ttgagaccgc cgttatgcgg cagataggtc ttcatcatgg gccgctgtcc
     2761 gaatgaggca atttgacgtg ggctagagcg ttttcaggaa aaagtggaaa ccggttttcc
     2821 gtccgaaaac gcgacaaact aagaatctag agtatttcat cgtttccgtg aaacggtgaa
     2881 atgctctagg cgcgacggcg tgcgtgcgcg aggcgacctt agcctcatgc caatccggga
     2941 gctaacggtt tttgccgagg cattctgtca gggactgcgc cagcgcgcgc agcagttcgg
     3001 gatagagcgc cggtccgggt ttcaggtcgg cgccgagcgg atcgagtacg ccgatgcgca
     3061 cgcttgtgcc ttcgcccaag gcgccgatca gcttgggcgg aaactgcggc tcccggaaaa
     3121 tgcaggcgac gcgaccctgg aggagcaccg cgcgcagggc cttgatgcgg cgcggtccca
     3181 ccggacggtc gggggccacc gtcaccgccc ccgccggctt cagcccgtaa cgggcctcga
     3241 aatagcggta agcgtcgtgg aagacgagat acggccggtc ccgcacaggc gccagcttcg
     3301 ccgccagctc ccggtcgaga gccttgagcg cggcgatctc gcgggcggcg ttggcgcggt
     3361 aggccgctgc gtgcgccggg tcgagctgga tgagcttggc ggcgatggcc tcggtcatcg
     3421 cgacggcgtt gcgcgggtcg agccacagat gcgggtcgtt cggcccatgg tcgtgttcgt
     3481 cggcgtcctc ccatagccct ccgtgccggg ccggcagcag gcgcacgccg ggcgcccgtt
     3541 ccagcaccac gacccgacct ccggcgccga gcgccgccaa cggcgcggtc agataggttt
     3601 ccaggtccgg accgatctcg aagatcgcgt ccgcgcgctc gatcttgcgg gcgtcggagg
     3661 gcttgagcgc ataggaatgt tccgacaggg cgccgccgat caaaagctcg ggttcgccca
     3721 ccccttgcat cacggcggcg gtgagcgaat ggaccggctt gatcgtggcc agcactttgg
     3781 gggcggcggc ggcgggcatc gcccccaaca gcagggccag ggccacgcag atttctttca
     3841 gcttcagcat cgtcgtttcg gctagtgtcg tggtttcgaa gttcgcacca ttcgttatat
     3901 cgggctgcaa gcgaacttcg aaaccaaaac gacactagga ttcaataggt tgctagtgtc
     3961 ctttcgattc cgaaattcgc ccgacgccga tatagagatg ca
//
LOCUS       DASWPU010000147         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_25492,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000147 DASWPU010000000
VERSION     DASWPU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25492"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VGC27_03320"
     CDS             complement(<1..649)
                     /locus_tag="VGC27_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY0281637.1"
                     /translation="MRDPYEILGVPKGASEAEIKKAFRTLAKKHHPDTKGGDMAAKRR
                     FQEISAAYDILGDKEKRAKFDAGQIDAGGNPRGFDPRSHGFEGGPFGQGGGGPGDFHF
                     TWTNQGRRQESDAAEGFRPEDLFADLLGGLGGRGRGRARAQPRAGQDFAVRTAVSFEE
                     AAHGGTRRVVLPNGEQIDVKIPPGLKDGQQIRLKGRGGAGKNGGPPGDVMIHVAVA"
     gene            complement(710..1273)
                     /locus_tag="VGC27_03325"
     CDS             complement(710..1273)
                     /locus_tag="VGC27_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281638.1"
                     /translation="MRKLAALPALCLAALLGGCASDALSGPAIQAIFAEGLKDYDSGN
                     YEAAYKKWSSIDDMDLAAMRNVGAMLRTGKGVPKDPKAALGMLTRAAKAGLFTAQADV
                     GDMLLKGEAGPPDPKAAAPWLAMAARAGHPMAQYQLGQLYEQGLGVPKDIEAARALYT
                     AAAAGGVADAGGRLETLPPKPSPALRH"
     gene            1321..1947
                     /gene="pdxH"
                     /locus_tag="VGC27_03330"
     CDS             1321..1947
                     /gene="pdxH"
                     /locus_tag="VGC27_03330"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY0281639.1"
                     /translation="MSEIGGPVDDGGIAPARDPFELFAEWMKAAEKTEPNEANAMAVA
                     TVDAAGRPNVRMLLLKGVDDRGFVFYTNMQSTKGSELAVHPFAALDFHWKTQRRQVRV
                     RGPVSEVSAEEADAYFASRAKDSQIGAWASAQSRPMEGRWVFEKEIAKFALKYALTKV
                     PRPPHWSGYRVAPLQIEFWRDRPFRLHDRLVYRRESVGKPWRTERLFP"
     gene            1944..2873
                     /locus_tag="VGC27_03335"
     CDS             1944..2873
                     /locus_tag="VGC27_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0281640.1"
                     /translation="MIAAASPSDRTVSKHGVLMRRVALAAVVTAMFLVALKTVAFILT
                     DSMAMMASLADSSLDVFASFINLLAVRAALTPADREHRFGHGKAEPLAGLAQSAFIAG
                     SSAFLVIESINRMLQPRPIEHGTAGLVVMGISIVATVILVTAQQIVVRRTGSIAISAD
                     RMHYISDILVNVGVVIGIVLASNFNILIADPIVGLLVAGVLAVGVWRVFSRSYDQLMD
                     RELPDADRERIKRIVMQNPAVRDMHDLRTRAAGVHAFIQVHIELDPSLSLMAAHAVAD
                     AVEVAVAEAFPNSEVIIHQDPEGLEAIAALAKS"
     gene            3041..>4050
                     /gene="uxuA"
                     /locus_tag="VGC27_03340"
     CDS             3041..>4050
                     /gene="uxuA"
                     /locus_tag="VGC27_03340"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF003027.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEY0281641.1"
                     /translation="MKMTFRWYGDSDPVPLAYIRQIPGIDGIVSAVYDVPVGEVWPLE
                     NIRTLKQTIESAGLKFEVIESVPVHEDIKLGRPTAVRLIANYAQTLRNLAQCGLKVVC
                     YNFMPVFDWTRTILDMKLEDGSSALAFDVDVVNKIDPEQGINLPGWDASYKPEELKAL
                     LAEYRGVGEERLWGNLAAFLKGVIPAAEEAGIKMAIHPDDPPRPIFGLPRIVKNAEDL
                     RRLLKIVDTPANGLTLCSGSLGADIHNDIVALVKEFAGAGRINFAHIRNVKVDAKGNL
                     HETAHKTDCGSLDIAGIVKAYYDAGYEGYVRPDHGRMIWGETGKPGYGLYDRALGAVY
                     LNG"
BASE COUNT          659 a         1335 c         1328 g          728 t
ORIGIN      
        1 gcgccaccgc gacatggatc atcacgtcgc ccggcggccc gccgttcttg cccgcgccgc
       61 cgcggccttt cagcctgatc tgctgtccgt ccttgaggcc gggcggaatc ttgacgtcga
      121 tctgttcgcc gttgggcagg acgacgcgcc gggttccgcc atgcgcggct tcctcgaagc
      181 tgacggcggt ccgcaccgcg aaatcctgtc cggcgcgcgg ctgggcgcgt gcgcggccgc
      241 ggccgcggcc gcccaggccg ccgagcaggt cggcgaacag atcttcgggg cggaagccct
      301 cggcggcatc gctctcctgt ctgcggcctt gattggtcca ggtgaaatga aagtcgcccg
      361 ggccgccgcc gccctggccg aacggcccgc cctcgaaacc gtggctgcgc ggatcgaaac
      421 cgcgcggatt gccgccggcg tcgatctggc cggcatcgaa cttggcgcgt ttttccttgt
      481 cgcccaggat atcgtaggcg gcgctgatct cctgaaaacg ccttttcgct gccatatcgc
      541 cgcccttggt gtcggggtgg tgcttcttgg cgagcgtccg gaacgccttc ttgatctcgg
      601 cttcgctggc gcctttgggg acgcccagga tttcataagg gtctcgcatg ccttctccag
      661 ccgccggaat cggtggcatt tacaatatag ggtccaaatt cggcgcgcgt taatgcctta
      721 gggcgggcga gggtttgggc ggcagcgttt ccagccgtcc gccagcgtcc gccacgccgc
      781 ccgcggccgc ggccgtgtaa agggcccgcg ccgcttcgat atctttcgga accccaagcc
      841 cctgttcgta aagctgaccc agttggtact gcgccatggg gtgtccggcc cgtgcggcca
      901 tcgccagcca gggcgcggcg gctttgggat cgggcgggcc ggcctcgccc ttgagcagca
      961 tgtcgccgac atcggcctgg gccgtgaaca gccccgcttt ggcggcgcgt gtcagcatcc
     1021 ccagagccgc cttagggtct ttggggacgc ccttgccggt gcgcagcatg gcccccacat
     1081 ttcgcatggc ggccaggtcc atatcgtcga tcgacgacca tttcttgtag gccgcttcgt
     1141 aattgcccga atcgtagtcc ttcagtccct cggcgaagat ggcctggatg gcggggccgg
     1201 aaagggcatc gctggcgcat ccgcccagca gggcggcgag gcacagggcg ggcaaggcgg
     1261 cgagcttacg cattgccggg atgctagcag accggcgctt gaaggtgcta tgcggttttc
     1321 atgagcgaga tcggcggacc tgttgacgac ggcggcattg cgcctgcgcg cgacccattc
     1381 gagctgtttg ccgagtggat gaaggcggcc gagaagaccg agccgaacga ggccaacgcc
     1441 atggccgtgg cgacggtgga cgcggccggc cggcccaatg tgcgcatgct gctgctgaaa
     1501 ggcgtggacg accgcggctt cgttttctac accaacatgc agagcaccaa gggcagcgag
     1561 cttgccgttc atccgttcgc ggcgctcgac ttccactgga agacccagcg ccgccaggtg
     1621 cgggtgcgcg ggccggtcag cgaggtcagc gccgaggagg ccgacgccta tttcgcctcg
     1681 cgcgccaagg acagccagat cggcgcctgg gcttcggcgc agtcgcggcc gatggaaggg
     1741 cgctgggtgt tcgagaagga gatcgcgaag ttcgcgctga aatacgcgct gacgaaagtt
     1801 ccgcggccgc cccattggtc cggctatcgc gtcgctccgc ttcagatcga attctggcgc
     1861 gaccgtccgt tccgcttgca cgaccggctg gtctatcgcc gcgagagcgt cggcaagcct
     1921 tggcgcacgg agcgcctgtt cccgtgattg cggcggcctc cccttccgac cggacggttt
     1981 ccaagcatgg cgttctgatg cgccgggtgg cgctggccgc ggtcgtcacc gcgatgttcc
     2041 tggtggcgct gaagacagtg gccttcatcc tcaccgactc gatggcgatg atggcctcgc
     2101 tggccgattc ctcgctcgac gtcttcgcct ccttcatcaa tttgctggcg gtgcgcgcgg
     2161 cgctgacgcc cgccgaccgc gagcaccgtt tcggccacgg caaggccgag ccgctggcgg
     2221 gtctggcgca aagcgccttc atcgccggtt cgtcggcctt cctggtgatc gaatccatca
     2281 accgcatgct tcagccgcgc ccgatcgaac acggtacggc cggcttggtg gtgatgggta
     2341 tttcgattgt cgcgaccgtg attctggtca ccgcgcagca gatcgtggtg cggcgcaccg
     2401 gctcgatcgc catcagcgcc gaccgcatgc attacatcag cgacatcctg gtcaatgtcg
     2461 gcgtggtgat cggcatcgtc ctggcgtcga acttcaacat cctgatcgcc gatccgattg
     2521 tcgggctcct ggtcgccggc gtgctggcgg tcggggtgtg gcgcgtcttc agccggagct
     2581 acgaccagct catggaccgc gagctgcccg acgccgaccg cgaacgcatc aagcggatcg
     2641 tcatgcaaaa ccccgcggtg cgcgacatgc acgatctgcg cacccgcgcg gccggcgtcc
     2701 acgccttcat ccaggtccac atcgaactcg atccgtcgct tagcctgatg gcggcgcatg
     2761 cggtggctga cgcggtggag gtcgccgtcg ccgaagcctt ccccaactct gaagtgatca
     2821 tccaccagga cccggaaggc cttgaagcga tcgccgccct ggcgaagtct tagtttccgc
     2881 cccgccccgg ggaaaccgcc aaggcgatat attatctgtg tacagatcaa tcgccctgcg
     2941 tcatctgggc ggcccgcatc cgcgggtcat gacaaaggcg ggaattgcgg gttaggatgg
     3001 gatcgccgcc acacggggcc gggcataagg aggacgcgca atgaaaatga ctttccgctg
     3061 gtatggcgac agcgacccgg tgcctctggc ctatatccgc cagattcccg gcatcgacgg
     3121 catcgtctcg gcggtctacg acgtgccggt cggcgaagtg tggccgctgg agaacatccg
     3181 caccctcaag cagaccatcg aatccgccgg cctcaaattc gaggtgatcg aaagtgttcc
     3241 ggtgcatgag gacatcaagc tgggccgccc cacggccgtc cgcctgatcg ccaactacgc
     3301 ccagacgctg cgcaacctgg cgcaatgcgg gctgaaagtg gtctgctaca atttcatgcc
     3361 ggtgttcgac tggacgcgca ccatcctcga catgaaactc gaggacggct cgtcggcgct
     3421 ggcgttcgac gtcgacgtcg tgaacaagat cgatccagaa cagggcatca atcttcccgg
     3481 ctgggacgcc agctacaagc cggaggaatt gaaggcgctg ctggccgaat atcgcggcgt
     3541 cggcgaggaa aggctatggg gtaatctcgc cgccttcttg aagggcgtga tccccgctgc
     3601 cgaggaagcc ggcatcaaga tggcgattca tcccgacgat ccgccgcgcc cgattttcgg
     3661 cctgccgcgc atcgtcaaga acgccgagga tctgcgccgc ctgctcaaga tcgtggacac
     3721 gccggccaac gggctgacgc tatgctccgg ctcgctcggc gccgacatcc acaacgacat
     3781 cgtcgcgctg gtgaaggagt tcgcgggcgc cgggcgcatc aacttcgcgc acatccgcaa
     3841 cgtcaaggtg gacgcgaagg ggaatttaca cgagacggcg cacaaaaccg attgcggttc
     3901 gctcgacatc gccgggatcg tgaaagccta ttacgacgcg ggctatgaag gctatgtgcg
     3961 ccccgaccac ggccgcatga tctggggcga aaccggcaag cccggctacg gcctctacga
     4021 ccgcgccctc ggcgcggtgt atctcaacgg
//
LOCUS       DASWPU010000148         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_80522,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000148 DASWPU010000000
VERSION     DASWPU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80522"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..1378
                     /locus_tag="VGC27_03345"
     CDS             284..1378
                     /locus_tag="VGC27_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0281642.1"
                     /translation="MQKRLRNISDADFDRFLNFATDHPAIVKRDHLLQKLTIFNFVAP
                     VVERHKPETILEIGCGLGFHSALLTNYGRVFATELEVPGSFAAIDTNVSGGRATVFRE
                     LAKSEIRFATNDGRTIPFPDRSFDLIFHNSVIEHVPDPAAFNRETWRLLKPNGIVICI
                     TGTPALCRLRFIRDYLLRLPITMAGALVKESGLTRRLKISDQIKALLPKGVSPPSPAP
                     SVSGWYARLFHYVYSPIYNAIVLDELARSAGLGKDEALAVAYDHFRNSAFNRVRYYLT
                     PRTHGQHYRNFLHEMSEWKPDRWRGTFTTAGFDVVAHVPYRFHHLLETTWSGKINSAL
                     YHWAAPLIERYHRSIPPGLGSEFVLVARKN"
BASE COUNT          293 a          466 c          445 g          322 t
ORIGIN      
        1 gccggcgatc caatctacgg cggcccctgc aatggcgcaa ttgcctgtgg agaggagagc
       61 tttgggatcg gtgctggcgg acaaaaactg ttggcaacat cggagaaatt atataattga
      121 cctcccgcat gctgttatgc atggggctgg accatgctgg ggttcgtgcc ttgtcccatg
      181 gcatcaccga acctgtaagg cgcaaatgat gtccggcccg gcctccaacc ggatgaaggg
      241 gggagctaag gccgccgtcc atccgtgttg aggcattcga cagatgcaaa aacggcttcg
      301 caacatctcc gacgcggatt tcgaccgttt tctgaacttc gcaaccgacc atcccgcgat
      361 cgtcaagcga gaccacctgc tgcagaagct gacgatcttt aacttcgttg cgccggtcgt
      421 cgagcgccat aaaccggaga cgatcctcga gatcggctgc ggtctcggct ttcattccgc
      481 tctgctgacg aactacggcc gggtcttcgc gacggagctg gaagtgcccg gaagcttcgc
      541 cgccattgat acaaacgttt ccggcggccg cgccaccgtg ttccgggaac tggcgaaaag
      601 cgagattcgg tttgcgacca atgacggcag gacaattccg ttcccggatc gctcgttcga
      661 tctcatcttc cacaattccg tgatcgagca tgttcccgat cccgccgcgt tcaaccgcga
      721 gacttggcgg ttgttgaagc cgaacggcat cgtgatctgc atcacgggta cgccggcgct
      781 ttgccggctc aggttcattc gcgattacct cttgaggctg ccgatcacga tggcgggggc
      841 gcttgtcaag gaatcgggcc tgacgaggcg tctgaagatc tccgatcaaa tcaaggcgct
      901 gcttccgaag ggcgtgtcgc cgccttcgcc tgcgccctcg gtttccggct ggtacgcgcg
      961 gctgtttcat tacgtctaca gcccaatcta caacgccatt gtgctcgacg agttggcgcg
     1021 aagcgccggg ctcgggaaag acgaggcgct cgccgtcgcg tacgaccact ttcgaaactc
     1081 cgccttcaat cgggttcgct attatttgac gcccaggacc cacggtcagc actatcgcaa
     1141 cttcctgcac gaaatgtcgg agtggaaacc ggatcgttgg cgcggcactt ttacgacggc
     1201 cgggtttgat gtcgtcgcgc acgtccctta cagattccac cacctgctcg aaacaacgtg
     1261 gagcggcaag atcaactcgg cgctttacca ttgggcggcg cccctgatcg agcggtacca
     1321 ccggtcgatc ccccccggcc tcggttcgga attcgtcctc gtggcgcgaa agaactgacc
     1381 gccggagcgg attagatcgg atcgacgctc ctggccgggc tcgctccggc aaaaggcatg
     1441 gcaagttgca aaaagcctgc ttttgccgca tgctcgggtt cacatcggta tccactctgt
     1501 ttcacgagcc gcgccgagtt tgctgg
//
LOCUS       DASWPU010000149         6678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_117375,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000149 DASWPU010000000
VERSION     DASWPU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117375"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="VGC27_03350"
     CDS             <1..1609
                     /locus_tag="VGC27_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HEY0281643.1"
                     /translation="EAEGELRKMTLSEDTRPSRHSRLRQRIIVINIIVFLLLIAGVLA
                     VQSSREGLVDERITGIEEQARVVANTLAEYATVSDTRSLKVDDAAPLLRELLAPTRLR
                     GRLYDTSGQLVIDSRNLLARNIVQTLELPPIDDWSRFKLSVSRFYDGVVGVRPLVRSE
                     PYFEAGKDGRVYREVGKALQGNIASAERVDEQNKLILSVAVPVQRFKAIYGVLFVTTE
                     GGDIDDILRQERATLIKYFLLAFAVMLVSGFYLSGTIAEPMRKLAEAADRVRQGRAGR
                     ETLPDISDRNDEIGDLSQSLSAMTRALYDRIDAIERFAADVAHELKNPLTSLKSAVEM
                     FSLAVDDESRARLMAIVRTDVKRIDRLITDISDASRLDAELSREPSEPVDISGLLKAV
                     AEIYHFMELPRGVKLALDLRLPPEAFVMGRDERLGQVVRNLIDNAVSFSPEGGTVTAT
                     AWAEGTTIRIMVEDDGPGIPPDNLESVFERFYTERPVAQGADKNSGLGLSIARQIVTG
                     LRGRIWAENREQGGARFVVELPRARLQ"
     gene            1606..2112
                     /locus_tag="VGC27_03355"
     CDS             1606..2112
                     /locus_tag="VGC27_03355"
                     /inference="COORDINATES: protein motif:HMM:NF019118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0281644.1"
                     /translation="MSRNARFNIHATCVRLGRAGAAFGAPPDAGVLLLGKSGAGKSDL
                     ALRLIALGAALVADDRTDLYVWHRRLYARPPARLAGLIEIRGIGILKLPHAPRARVAL
                     VVELGRGAKRLPDHRRYRPPAGLALPAAAAPPIVKIVPFEASATAKIVAVTAAYALGL
                     YRDTFNPT"
     gene            2167..2574
                     /locus_tag="VGC27_03360"
     CDS             2167..2574
                     /locus_tag="VGC27_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY0281645.1"
                     /translation="MIGIVLVTHGRLAQEFISATEHMVGPQTHLRAVCIGPEDDIERR
                     QREIAAAVKSVDLGKGVVIATDMFGGTPCNLSLTLLEKGKIEVLAGVNLPSLIKLIDA
                     RTKMPLEQAVKEAIDAGRKYMRAGSADLVGSGV"
     gene            2580..2861
                     /locus_tag="VGC27_03365"
     CDS             2580..2861
                     /locus_tag="VGC27_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY0281646.1"
                     /translation="MPDAARLSAVARITNKRGLHARASAKLVEAASRFKSDITVSKDG
                     QSVEARSIMGLMLLAASLGTEVEISAKGEDAKEALTAILALIKANFGEE"
     gene            2869..3756
                     /locus_tag="VGC27_03370"
     CDS             2869..3756
                     /locus_tag="VGC27_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY0281647.1"
                     /translation="MKRRAALFVLAFLTAFVPARAEAPQTVDAHPALWRVHGEGGTAY
                     LFGSLHMLPPNVNWQTPEIKRAFSHADTYVFEIPLGADTINRVNALMGERGMLPPGQS
                     LRAMLPPDSQADLDKVLASFQLPLTAVDNKRPWLVSLMLASIMAKKHELQDAFGADKI
                     LGAQAKASGKPILYLETVDQQLALVAPDDPKVELQFFEAGLKSFATADNDIAAITNAW
                     EKGDIAALDAVTAKEFKDQPDARAAYFTDRNRAWTKQIEAMLRERRTFFIAVGAGHLA
                     GKDGVPALLRADGYKVDGP"
     gene            complement(4146..4889)
                     /gene="pflA"
                     /locus_tag="VGC27_03375"
     CDS             complement(4146..4889)
                     /gene="pflA"
                     /locus_tag="VGC27_03375"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HEY0281648.1"
                     /translation="MHGTGVNVEGYVHSVVTGGAVDGPGLRFVVFLSGCHLRCLYCHN
                     PDTWHMKDGKKTGVAHLLNEILLYSHYLKRTGGGVTASGGEPLAQPEFVTALFQGCKA
                     LGLHTALDTNGYLGEDASQALLDATDLVLLDIKAWNPEVYKKLTGAELAPTLRFAERL
                     SAMGKATWLRYVLVPGWTDDLESIDGLAAYAAKLKMEKVEVLPFHKMGEPKWEAMRLP
                     YMLKDTQPPSPELVAKVKSIFVGHGLKTV"
     gene            complement(4901..6658)
                     /gene="pflB"
                     /locus_tag="VGC27_03380"
     CDS             complement(4901..6658)
                     /gene="pflB"
                     /locus_tag="VGC27_03380"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEY0281649.1"
                     /translation="MITGLPDGYGRGRIIGDYRRVALYGVDRLIAERKDQFAQLDGLI
                     NEDTIRLREELAEQVKALKELKAMAKSYGFDISRPAENAREAVQWTYFAYLGAVKEQN
                     GAAMSIGRVSAFLDIYFERDLQTGVLSESEAQELIDHLVMKLRMVRFARTTEYNELFS
                     GDPTWVTEVLGGMGLDGRTLVTKTTFRFLHTLTNLGPAPEPNLTVLWSVNLPEAFKKY
                     CAQVSIETSSLQYENDDLMRTFWGDDYGIACCVSAMRIGKQMQFFGARANLAKTMLYA
                     INGGRDELSGEQVGPRFAPIAGEVLDYDEVMSRFDTMMDWVAGTYMDALNIIHYMHDK
                     YCYERLEMALHDRDILRTMACGIAGLSIAADSLSAIKYAKVRPIRDARGLATEFEIEG
                     EFPCYGNNDERADSIAVELVERFMSKLKTKHTYRGAVPTQSVLTITSNVVYGKKTGGT
                     PDGRKAGEPFAPGANPMHGRDKKGALASLSSVAKLPYESAQDGISNTFSIVPGALGKT
                     RHGRKDNLVGLLDGYFEESGHHLNVNVFDRETLMDAMDHPEKYPQLTIRVSGYAVNFI
                     KLTREQQLDVIHRTFHTSI"
BASE COUNT         1243 a         2113 c         2080 g         1242 t
ORIGIN      
        1 ggaagccgaa ggcgagcttc gaaaaatgac gctgtccgaa gacacgcgcc cttcgcgcca
       61 ttccaggctg cgccagcgca tcatcgtcat caacatcatc gtcttcctgc tcttgatcgc
      121 cggggtgctg gcggtgcaat ccagccgcga gggcctggtc gacgaacgga tcaccggcat
      181 cgaggaacaa gcccgcgtcg tggccaacac gctggcagaa tacgccaccg tcagcgacac
      241 gcgctcgctc aaggtcgacg acgcggcgcc gctgctgcgc gaactcttgg cgcccacacg
      301 gttgcgcgga cggctgtacg ataccagcgg gcaattggtg atcgattcgc gcaacctgct
      361 ggcgcgcaac atcgtacaga ccttggagct gccgccgatc gacgattgga gccgcttcaa
      421 gctgtcggta agccgcttct acgacggcgt ggtgggcgtg cgcccgctcg tcaggtccga
      481 gccttatttc gaagcgggca aggacgggcg cgtttatcgc gaagtcggca aggctttgca
      541 gggcaacatc gcctcggccg agcgcgtcga cgaacaaaac aagctgatcc tctccgtggc
      601 cgtaccggtg cagcgcttca aggccattta cggcgtgttg ttcgtcacca ccgaaggcgg
      661 cgatatcgac gacatcctgc gccaggagcg cgcgacgctg atcaaatatt tccttctggc
      721 gttcgcggtg atgctggttt ccggcttcta tctgtctggc accatcgccg aaccgatgcg
      781 caagctcgcc gaagcggccg accgcgtgcg ccagggacgc gccggacgcg aaactcttcc
      841 cgacatctcc gaccgcaacg acgagatcgg cgatttgtcg cagagcctgt cggccatgac
      901 gcgcgcgctc tacgaccgta tcgacgccat cgagcgcttc gccgccgatg tggcgcacga
      961 gctgaaaaac cctcttacct cgctcaagag cgcggtggag atgttctcgc tggcggtgga
     1021 cgacgaatcc cgcgcgcggc tgatggccat cgtgcgcacc gacgtcaaac gcatcgaccg
     1081 gctgatcacc gacatttccg acgcttcgcg cctcgacgcc gagttaagcc gcgagccgag
     1141 cgagccggtc gatatctccg gcctgctcaa ggccgtcgcc gagatctatc acttcatgga
     1201 actgccccgc ggggtgaaac tcgccctcga ccttcgcctg ccgccggaag cgttcgtcat
     1261 gggccgcgac gagcggctgg gacaagtcgt gcgcaatctc atcgacaacg ccgtgtcgtt
     1321 cagtccggaa ggcggcaccg tgaccgccac ggcatgggcc gaaggcacga cgatacgcat
     1381 catggttgag gacgacggcc ccggcatccc gcccgacaat ctcgaatccg tattcgagcg
     1441 tttctacacc gagcggccgg tcgcacaggg cgccgacaaa aattccggcc ttggcctttc
     1501 catcgcgcgg cagatcgtga ccggcctgcg cgggcgcatc tgggccgaaa accgcgaaca
     1561 aggcggcgcg cgcttcgtcg tggagctgcc aagggcacgc ctccaatgag ccgcaacgcg
     1621 cgtttcaata tccatgcaac ctgtgtgcga ctgggccgcg ccggcgccgc cttcggcgct
     1681 ccgccggatg cgggcgtgct cctgctcggc aaaagcggcg cgggaaaatc agaccttgcg
     1741 ctcaggctga tcgcgctggg ggccgcgctg gtcgccgacg accggaccga tctgtatgtc
     1801 tggcaccgga gactttatgc ccgcccgccc gcccgcttgg ccggcctgat cgagatccgc
     1861 ggcatcggca ttctgaagct gccgcatgcg ccgcgcgcgc gcgttgccct ggtggtggaa
     1921 ctggggcggg gcgcgaagcg gcttcccgac catcgccgct accgcccgcc cgccggcttg
     1981 gcgctgcctg cggcagcggc gccgccgatc gttaaaatcg tcccgttcga ggccagcgcg
     2041 accgctaaaa tcgttgccgt tacggcggct tatgcccttg gcttgtatcg cgacactttc
     2101 aaccctactt agggctttcc tcacacgtcg ggatcgttat cgtgcgcccc gcaaacggac
     2161 acactcatga tcggcatcgt tctcgtcacc catggccgtc tggcacaaga gttcatttcg
     2221 gccaccgaac atatggtcgg cccgcagacg catctgcgcg ccgtctgcat cggccctgaa
     2281 gacgacatcg aaagacgcca gcgcgaaatc gccgcggcgg tgaaatcggt cgatctcggc
     2341 aagggcgtgg tgatcgccac cgacatgttc ggcggcacgc cgtgcaacct gtcgctgacg
     2401 ctgctcgaaa aaggcaagat cgaggtgctg gcgggggtca acctgcccag cctcatcaag
     2461 ctgatcgatg cgcgcaccaa gatgccgctg gaacaggcgg tgaaggaagc gatcgacgcc
     2521 gggcgcaaat atatgcgggc aggatccgcc gaccttgtcg gcagcggggt ctaaaaccga
     2581 tgcccgacgc cgcgcgtctt agcgctgtcg cccggattac aaacaagcgt ggactgcatg
     2641 cgcgcgcctc cgccaagctg gtggaagccg cctcccgctt caagtccgat atcacggtta
     2701 gcaaggacgg ccagtcggtc gaggcgcgct cgatcatggg cctgatgctg ctggccgcga
     2761 gcctgggaac cgaagtggaa atctcggcca agggcgaaga cgcaaaagaa gcgctgaccg
     2821 ccatcctggc gctgatcaag gccaatttcg gcgaggagtg atagcaacat gaaacgccgc
     2881 gccgcgcttt tcgttctcgc ttttctcacc gcattcgtgc ccgcgcgggc cgaagcgcct
     2941 cagaccgtcg atgcccatcc ggcgctgtgg cgcgtccatg gcgagggcgg aaccgcttat
     3001 ctgttcggct cgctccatat gctgccgccg aatgtgaatt ggcagacgcc ggagataaaa
     3061 cgcgccttca gccacgcgga cacgtacgtt ttcgagatac cactcggcgc cgacacgata
     3121 aaccgggtca acgcgttgat gggcgagcgc ggaatgcttc cgcccggtca aagcctgcgt
     3181 gccatgttgc cgccggacag ccaggcagat ctcgacaagg tcctcgcttc gttccaacta
     3241 ccgctgacgg cagtggacaa caagcggccc tggctggttt cgctcatgct tgcttccatc
     3301 atggcgaaga agcacgaact gcaagacgca ttcggtgccg acaaaatcct cggcgcgcag
     3361 gcgaaggcga gcggcaagcc gattctctat ctggaaacgg tggaccagca gctcgcgctg
     3421 gtcgcgcccg acgatcccaa ggtcgaactg caatttttcg aggccgggct gaaatccttc
     3481 gcaaccgcgg acaacgatat tgcggctatc accaacgcct gggaaaaggg cgacatcgcc
     3541 gcgctggatg ccgtcacggc caaggagttc aaggaccaac ccgacgcgcg cgccgcgtac
     3601 ttcacggacc gcaatcgcgc ctggacgaag cagatcgaag ccatgctgcg tgaacgcagg
     3661 accttcttca tcgcggtcgg cgccggtcat ctggccggca aggacggcgt gccggcgctc
     3721 ctgcgcgccg acggctacaa ggtcgacggg ccgtaagaaa ttgccctcac gtgaaggcgg
     3781 tttatcatca agatgacgaa cataaaccct atgtcttggc ctgcccatgt gcgagccact
     3841 caggtggcga tggcgcgcag cgtaagaaat ttcactgcat tcttttttga taatttcggc
     3901 aggtttcaac tggatggccc gcagtcgcgg gccatgacaa acgagtattt gtttccttca
     3961 gattacaggc gcctctagtg tcccgattcc gaaattcgcc ccatctctat atcggcttcg
     4021 ggcgaatttc ggaatcgaaa ggacactaga aacctattga atcctagtgt cgttttggtt
     4081 tcgaagttcg cttgcagcca gatatcgcgg atggtgcgaa cttcgaaacc acgacactag
     4141 agaaatcaga ccgtcttcaa tccgtgcccg acaaagatac tcttgacctt ggcgaccagt
     4201 tcgggtgacg gcggctgggt gtccttgagc atatagggaa ggcgcatcgc ttcccatttc
     4261 ggctcgccca tcttgtggaa gggcaggact tccactttct ccatcttcag cttggccgca
     4321 taggccgcca gcccgtcgat ggattccaaa tcgtcggtcc agccgggcac cagcacgtag
     4381 cgcaaccagg tggccttgcc cattgccgac aggcgttcgg caaagcgcag cgtgggggcg
     4441 agttcggcgc cggtcagctt tttatagacc tcggggttcc acgctttgat gtcgagcagc
     4501 accagatcgg tggcatcaag tagcgcctgg ctggcgtctt cgcccagata gccgttggta
     4561 tcgagtgcgg tgtgcagccc caacgcctta cagccctgga acagcgcggt gacgaattcc
     4621 ggctgcgcca gcggctcgcc gccggaggcg gtgacgccgc cgccggtgcg tttcaagtaa
     4681 tgggaataaa gcaggatttc gttgagcaga tgggcgacgc ccgtcttctt gccgtccttc
     4741 atgtgccagg tgtcggggtt gtggcaatag aggcagcgca aatggcagcc cgagaggaac
     4801 acgacgaagc gcagccccgg accgtccacg gctccgccgg tgaccaccga gtggacatag
     4861 ccttcgacgt tgacgcccgt cccgtgcacg gcggcactcc ttaaatgctg gtgtggaacg
     4921 tgcggtggat cacgtccaac tgctgttcgc gcgtcagctt gatgaagttg acggcgtagc
     4981 ccgacacccg gatggtcaat tgcggatact tctcggggtg gtccatcgca tccatcagcg
     5041 tctcgcgatc gaacacgttg acgttcaaat ggtgtccgga ctcctcgaaa tagccgtcca
     5101 gcaagccgac caaattgtcc ttgcggccgt gccgcgtctt gcccagcgcg cccggcacga
     5161 tcgagaaggt gttggagatg ccgtcctgcg cggattcgta gggcagcttg gcaaccgaac
     5221 tgagcgaggc cagcgccccc ttcttgtcgc ggccgtgcat cggattggcg cccggcgcaa
     5281 acggttcgcc ggccttgcgc ccatcgggtg ttccgccggt cttcttgccg tacacgacat
     5341 tggacgtgat ggtcaggacc gactgcgtgg gcaccgcgcc gcgataggtg tgcttggtct
     5401 tcagtttgct catgaatcgt tcgaccagct cgacggcgat ggaatcggcg cgctcgtcat
     5461 tgttgccgta gcaagggaac tcgccttcga tttcgaactc ggtggccagc ccgcgcgcgt
     5521 cgcggatcgg gcgcaccttg gcgtatttga tcgccgacag cgaatccgcc gcgatggaga
     5581 ggcccgcgat gccgcaggcc atggtgcgca ggatgtcgcg gtcgtgcagc gccatttcga
     5641 ggcgctcgta gcagtacttg tcgtgcatgt aatggataat gttgagcgca tccatatagg
     5701 tgcccgccac ccagtccatc atggtgtcga agcgcgacat gacttcgtca tagtccagca
     5761 cctcgccggc gatgggcgca aaacgcggcc cgacctgctc gccggaaagc tcgtcgcggc
     5821 cgccgttgat ggcgtagagc attgtcttgg cgaggttggc gcgggcgccg aagaactgca
     5881 tctgcttgcc gatccgcatg gcggaaacgc agcaggcgat gccgtaatcg tcgccccaaa
     5941 aggtgcgcat tagatcgtcg ttctcgtact gaagcgagct ggtttcgatc gagacttgcg
     6001 cgcagtactt cttgaaggcc tccggcaggt tcaccgacca caacacggtc aggttcggct
     6061 caggcgccgg gccgagattg gtcagcgtgt gcaggaagcg gaaggtggtt ttggtgacca
     6121 gcgtgcgacc gtcgaggccc atgccgccca gcacttcggt gacccaggtg gggtcgccgg
     6181 aaaacagttc gttatattcc gtggtgcggg cgaagcgcac catgcgcagc ttcatcacca
     6241 gatggtcgat gagttcctgc gcttcggatt cgctgagaac gccagtttga aggtcgcgct
     6301 cgaaatagat gtcgaggaaa gccgaaacgc ggccgatcga catggcggcg ccgttctgct
     6361 ccttcaccgc gccgagatag gcgaaatagg tccactgcac cgcctcgcgg gcgttctcgg
     6421 ccgggcgcga aatgtcgaag ccgtaggact tcgccatcgc cttcagctcc ttgagggcct
     6481 tgacctgttc ggccagttcc tcgcgcaggc ggatggtatc ctcgttgatg aggccgtcga
     6541 gttgcgcaaa ctggtccttg cgctcggcga tcagccggtc gacgccgtac agcgccacgc
     6601 ggcgatagtc gccgatgatc cgtccgcgcc cgtagccgtc cggcagcccg gtgatgatgc
     6661 cggccttgcg cgccttgc
//
LOCUS       DASWPU010000150         8003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_92937,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000150 DASWPU010000000
VERSION     DASWPU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92937"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /gene="hpnH"
                     /locus_tag="VGC27_03385"
     CDS             <1..684
                     /gene="hpnH"
                     /locus_tag="VGC27_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEY0281650.1"
                     /translation="KLAQSKGFRTQINCTLFQDAEPERVAKFFDEMTAMGLDGITISP
                     GYAYERAPDQEHFLNRARTRNLFRDILKRGNGGKKWQFTQSDLFLDFLAGNQTYECSP
                     WSMPARTVFGWQKPCYLLGEGYVKTFKELMEDTEWEKYGVGNYEKCANCMVHCGFEGT
                     AVADSVKHPYKLLPLALRGVKTDGPMAPDISLAGQRPAEFVFSRHVETKLAEIRATKP
                     GSEKVAAAS"
     gene            complement(628..1299)
                     /locus_tag="VGC27_03390"
     CDS             complement(628..1299)
                     /locus_tag="VGC27_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281651.1"
                     /translation="MSGTVIAVVGLAKEARIAKRAGLKPVIGGGDSHLLAERLEEAAP
                     GAAAVISFGIAGALAPLLRAGDAIVATHVVAGREHYACDPAWSQILRAKLANSRSAIV
                     TGVDEVIFYIGMKKALFRNTGAHAVDMESHVAARFAEKRGLPFAVLRVICDGAQRTLP
                     PATLEPLKPNGKVRLFTVLKSVARDPGQIPELVQTGGEAAKAFRALRRCSHLLGPGLG
                     CPYLG"
     gene            complement(1446..3398)
                     /gene="shc"
                     /locus_tag="VGC27_03395"
     CDS             complement(1446..3398)
                     /gene="shc"
                     /locus_tag="VGC27_03395"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEY0281652.1"
                     /translation="MKETSLAVEAVVRDATEALLKEQRADGHWVYELEADATIPAEYV
                     LLVHYLAEQPNLELERKIGVYLRRIQGEHGGWPLYHAGPPDISATVKAYFALKMIGDD
                     PDAPHMRRAREKILKRGGAAKTNVFTRILLALYGQTSWNKVPTVPVELILLPRWFPIH
                     LSKMSYWARTVIVPLLVLAAKKPLAKNPRDIQVRELFLPHGKAASKRAPHQKWVWSAF
                     FGALDVLLKWFEPFWPKGLRARAIGECVRWTTERLNGEDGLGAIYPAMANSVMMYDVM
                     GYPPTHPDRAVARKAVEKLLVVKDDEAYCQPCVSPVWDTALVCHALMEAHDPKADAAV
                     ARGLAWLKPLQVLDVKGDWAEEKPGVRPGGWAFQYNNAYYPDLDDTAVVVMALDRAKD
                     RAATAARVAPDVDVGEAIARGREWVEGLQSRNGGWGAFDADNSYYYLNNIPFADHGAL
                     LDPPTEDVSGRCIGMLAQLGEPHDSPRMKNAIAYLERVQLKDGSWFGRWGVNYIYGTW
                     SALAGLGAAGLDGQTPMVRRAADWLISIQNEDGGWGEDCASYRLDYSGYEPAPSTASQ
                     TAWALLGLMAAGEVDHPAVARGIAYLQRTQAADGLWPQEHYTGGGFPRVFYLRYHGYP
                     RFFPLWAVARYRNLKAGNSRRVGYGL"
     gene            complement(3552..4823)
                     /gene="hpnE"
                     /locus_tag="VGC27_03400"
     CDS             complement(3552..4823)
                     /gene="hpnE"
                     /locus_tag="VGC27_03400"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEY0281653.1"
                     /translation="MSTVYVVGGGLAGLSAAVALAEKGARVVLFEAAGQAGGRCRSYF
                     DAQLDCVIDNGNHLVLSGNRAIYDYLRTIGAEKALVGPQRAEFAFADLISGERWRFTP
                     NQGPLPWWIFDSTRRVPGTKPADYLRLTVLLTANPARRIDEVISCKGVLWERLLRPFL
                     LAALNTEPAGASAALAGAVLRETLAKGGRAYRPRIAHPTLAAAFVDPALAYLAAKDAL
                     VHFGQRLRRIVLDGDHAMALEFPESTLPLSPGDTVVLATPPWVTRELLSKVIVPDEFH
                     AIVNGHFKLAPPAGAAPMLGVVGGTAEWIFSFPDRISVTVSAADAIADKEREALAALL
                     WRDVAKVHGLSPALPPWQIVKERRATFAATPEQAAKRPQAKTEWRNLLLAGDWIDTGL
                     PATLESAVRSGQKAAQLALSRGGRSGEVDGG"
     gene            complement(4820..5734)
                     /gene="hpnD"
                     /locus_tag="VGC27_03405"
     CDS             complement(4820..5734)
                     /gene="hpnD"
                     /locus_tag="VGC27_03405"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEY0281654.1"
                     /translation="MTQSAISAPEIEAVRRKAATSSFYSAMRLMPKAEREAMFAIYAF
                     CRAVDDIADDGIGTRAERHAALDAWREDVKRLCAGGTPGQAAFLSETVARYRPRAEDF
                     LAVIDGMDMDVTEDIVAPGLATLDLYCDRVASAVGRLSIKVFGMEEGPGFTLAHHLGR
                     ALQLTNILRDLDEDAAIHRLYLPREFLAAAGVTIGEPFEALADPAIDKACREVAKLAH
                     GHYQEAGRILAAKPEGNLRAPRLMGAVYAEILHKMEAAGWAPPRRRVRIPKAQLAMIV
                     LRHGLERNPAKWEPVRRMIARPRKEPRA"
     gene            complement(5836..6753)
                     /gene="hpnC"
                     /locus_tag="VGC27_03410"
     CDS             complement(5836..6753)
                     /gene="hpnC"
                     /locus_tag="VGC27_03410"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEY0281655.1"
                     /translation="MSEAAAFSSGKGHRDENFPVASVFVAARHRPAILAFYRFVRTAD
                     DVADHAGASAEDKLRLLESMRLSLMGASDFSPEGAALRKLLAERGLTTDHAADLLEAF
                     RRDVTKLRYADWSELMDYCRYSAMPVGRYVLDVHGEDRAVWPLSDALCAALQVINHLQ
                     DCAKDYRSLDRIYLPLDIMAEHGATPDMLAGARATPALRSVIAALARRNAGLLTQSAP
                     FAAHIKDHRLALEVSLIQRLAEDLNAKLMVRDPLSERVHHRKLELPGFAVSAFARFLD
                     ARLRPSVVVSKFAPSALSGCKRTSKPKRH"
     gene            complement(6750..7889)
                     /locus_tag="VGC27_03415"
     CDS             complement(6750..7889)
                     /locus_tag="VGC27_03415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0281656.1"
                     /translation="MIAASLLALLSLAVWLGLLFGRGFFWQARERDDRNEPPEPATWP
                     SVAAVVPARNEADLIARSAGSLLAQDYPGSFRVILVDDQSGDGTGAAARALGSERLTV
                     LDGAAHPAGWTGKLFAVHQGVAQANAEDPPDYIWLTDADIAHAPDNLRRLVARAEANK
                     FVLTSLMAKLNCESFAERFLIPAFVFFFAMLFPFAWVNDPKKHAAAAAGGCMLVRREA
                     LARAGGIEAIRAEIIDDCALARRLKTQGPIWLGLTERAVSLRPYPRIADIRAMVARSA
                     YAQLGYSPLVLAGTVLGMALLTVVPLAGLAMGGLAPFFGTLAWAVMAAAFQPMLYFYR
                     RSPLWGLALPAIGVFYAAFTIDSAVQHWRGRGGMWKGRAQAITPA"
BASE COUNT         1450 a         2649 c         2618 g         1286 t
ORIGIN      
        1 aagctggcgc agtccaaggg cttccgaacc cagatcaact gcaccctgtt ccaggacgcc
       61 gagccggagc gtgtcgccaa attcttcgac gagatgacgg cgatgggtct ggacgggatc
      121 acgatatcgc cgggctacgc ctatgagcgc gcgcccgacc aggagcattt cctcaatcgc
      181 gcacgcaccc gcaacctgtt ccgcgacatc ctcaagcgcg gcaacggcgg caagaagtgg
      241 cagttcaccc agtcggacct gttcctggac ttcctggccg gcaaccagac ctacgaatgc
      301 tccccctggt cgatgccggc gcgcaccgtg ttcggctggc agaagccctg ctacctgctc
      361 ggcgaaggct atgtgaagac cttcaaggag ctgatggaag acacggagtg ggagaagtac
      421 ggcgtcggca attacgagaa atgcgccaac tgcatggtcc attgcggctt cgaaggcacg
      481 gcggtcgccg attccgtcaa gcacccctac aagctcttgc ccctcgcgct gcgcggcgta
      541 aagaccgacg gaccgatggc gcccgacatt tcgctcgcgg gccagcggcc ggcggagttc
      601 gtcttctccc gccacgtcga aaccaagcta gccgagatac gggcaaccaa gcccgggtcc
      661 gagaaggtgg ctgcagcgtc gtaaggcgcg gaacgccttc gccgcctcgc cgccggtctg
      721 gaccagttcc ggaatctgcc ccggatcgcg cgccaccgat ttcagcacgg tgaacagcct
      781 tactttgccg ttcggcttca gcggttccag cgtggcgggc ggcagcgtgc gctgcgcccc
      841 gtcgcaaatg acgcgcagga cggcgaaggg cagcccccgc ttttccgcaa agcgcgccgc
      901 gacgtgggat tccatgtcga cggcgtgcgc gccggtgttg cggaacaacg ccttcttcat
      961 gccgatatag aaaatgacct cgtccacgcc cgtgacgatc gccgagcgcg aattggcgag
     1021 cttggcgcgc aggatctgcg accaggccgg atcgcaggcg taatgttcgc gcccggcgac
     1081 gacatgggtg gcgacgatcg cgtcgcccgc cctgagcagc ggcgccaaag cgcccgcgat
     1141 gccgaagctg atgacggcgg cagcgcccgg ggccgcctct tccagacgct ccgccagcaa
     1201 gtgcgaatcg ccgccgccga tcacgggctt gagacccgcg cgtttggcga tccgcgcctc
     1261 tttggcaaga cccacgacgg cgatgacggt gccgctcatc tgaaacaagt atcttcaccc
     1321 tccccttgag ggccgccttc gctaaagcta cggcgtgcca agtgagattc acttgccacg
     1381 gcgaagccct taggcggagc cgggagggtt gaaaaatggc ctgtgatttt ccacctcgcc
     1441 atcgatcaca acccatatcc cactctgcgc gaattgcctg ccttcaggtt gcggtatcgc
     1501 gccaccgccc agagcggaaa gaatctcgga taaccgtgat agcgcagata gaacacgcgc
     1561 ggaaagccgc cgccggtgta atgttcctgc ggccaaagac cgtccgccgc ctgggttctc
     1621 tgcagatagg cgatgccgcg cgccacggcg ggatggtcca cttcgcccgc cgccatcagg
     1681 ccgagcagcg cccaagcggt ctgcgaggcg gtggacggcg caggttcgta gccggaataa
     1741 tcgagccggt agctggcgca gtcttcgccc cagccgccgt cttcgttctg gatggagatc
     1801 agccaatcgg cggcgcggcg caccatcggc gtctgcccgt cgaggcccgc cgcgcccagg
     1861 cccgccagcg ccgaccaagt gccgtagatg taattgaccc cccagcggcc gaaccagctt
     1921 ccgtccttca attgcacccg ttcgagatag gcgatggcgt tcttcatgcg cgggctgtcg
     1981 tgcggctcgc ccaactgcgc cagcatgccg atgcagcggc ccgatacatc ctccgtcggc
     2041 ggatcgagca gcgcgccgtg atcggcgaag ggaatgttgt tcaaataata ataggaattg
     2101 tcggcgtcga aggcgcccca gccgccgttc ctgctttgca gcccttccac ccattcgcgc
     2161 ccgcgcgcga tggcctcgcc gacatcgacg tccggcgcca ctctcgccgc tgtcgcggct
     2221 cgatctttgg cgcggtcgag cgccatcacc accacggcgg tgtcgtcgag atcgggataa
     2281 taggcattgt tgtactggaa ggcccagccg ccggggcgca cgccgggctt ttcctcggcc
     2341 caatcgcctt tgacatcgag cacctggagc ggcttcagcc aagcgaggcc gcgcgccacg
     2401 gcggcgtcgg ccttgggatc gtgcgcctcc atcagcgcat ggcagaccag cgccgtgtcc
     2461 cacaccggcg agacgcaagg ctggcaatag gcttcgtcgt ccttgacgac gagcagcttt
     2521 tccaccgctt tgcgggcgac ggcgcggtcg ggatgcgtgg gcggatagcc catcacgtcg
     2581 tacatcatca cgctgttggc catggcggga tagatggcgc ccagtccgtc ctcgccgttc
     2641 agccgttcgg tggtccagcg cacgcattcg ccgatggcgc gcgcgcgcag ccccttcggc
     2701 cagaacggct cgaaccattt gagcaggaca tcgagcgcgc cgaaaaaggc ggaccacacc
     2761 catttctgat gcggcgcgcg tttggaagcg gccttgccgt gcggcaggaa cagctcgcgc
     2821 acctgaatgt cgcgcggatt cttcgccagc ggtttcttcg ccgccagcac caagagcggc
     2881 acgatcaccg tgcgcgccca ataagacatt ttggagagat ggatcggaaa ccagcgcggc
     2941 aacaggatca gttcaaccgg caccgtcggc accttgttcc acgaggtctg gccgtacagc
     3001 gccagcagga tgcgcgtaaa cacattggtc ttggccgcgc cgccgcgctt gaggattttc
     3061 tcgcgcgcgc gcctcatgtg cggcgcgtcc ggatcgtcgc cgatcatctt cagcgcgaaa
     3121 taggccttca ccgtggcgct gatgtcgggc ggcccggcgt gatagagcgg ccagccgcca
     3181 tgctcgccct ggatgcggcg cagatagacg ccgatcttgc gctccagttc gagattgggc
     3241 tgctcggcca gataatgcac cagcaacaca tattcggcgg ggatggtagc gtcggcttcc
     3301 agctcgtaaa cccaatggcc gtcggcgcgc tgttccttga gcagcgcctc cgtggcgtcg
     3361 cggacgacgg cctccaccgc gagggaggtc tctttcatca ctgaaccaac ctcttcatag
     3421 ggaaccacat cgctctcacc cttcgacaag ctcatgcttc gacaagctca gcatgaagga
     3481 ttagccaatc ccctcaccct gagcttgtcg aagggcgagg gggcaccgaa taagtatcaa
     3541 tcgattgccg ctcagccgcc atccacttcc ccagacctgc ctccgcgtga caacgccaat
     3601 tgcgccgcct tttggcccga gcgcacggcg ctttcaagtg tggccggcag gccggtatcg
     3661 atccagtcgc cggccagcag gagattgcgc cattccgtct tcgcctgcgg ccgcttggcc
     3721 gcctgttccg gcgtggccgc gaaggtcgcg cgcctctctt tgacgatctg ccacggcggc
     3781 agcgccgggg acaggccgtg aaccttggcg acgtcgcgcc ataagagcgc ggcgagcgcc
     3841 tcgcgctcct tgtcggcgat ggcgtcggcg gcgctgaccg tcaccgagat gcgatccggg
     3901 aaggaaaata tccattcggc cgtgccgccc accacgccca gcatcggcgc cgcgcccgcg
     3961 ggcggcgcga gcttgaaatg cccgttgacg atggcgtgga attcgtcggg cacgataact
     4021 ttcgacagca gttcgcgcgt cacccagggc ggtgtcgcca gcaccaccgt atcgccgggc
     4081 gacagcggta gcgtgctttc gggaaattcc agcgccatcg cgtggtcgcc gtcgagaacg
     4141 atccggcgca gccgctggcc gaaatgcacc agcgcgtcct tggcggcgag ataggcgagc
     4201 gcgggatcga cgaacgcggc ggccagcgtg ggatgggcga tgcgcggacg ataggcccgt
     4261 ccgcccttcg ccagggtttc gcgcagcacc gctccggcca gcgcggcgga agcccccgcg
     4321 ggttcggtgt tgagcgccgc cagcaggaag ggcctgagca gccgctccca caagacgccc
     4381 ttgcaggaaa tgacttcatc gatgcgccgc gccgggttgg cggtcagcag taccgtcagg
     4441 cgcaagtaat ccgcgggctt ggtgcccgga acccggcgtg tggaatcgaa aatccaccag
     4501 ggcagcggcc cctggttggg cgtgaaccgc cagcgctcgc cgctgatgag atcggcgaaa
     4561 gcgaattcgg cgcgctgcgg cccaacgagc gccttctccg cgccgatggt gcgcagataa
     4621 tcgtagatcg cgcgattgcc ggaaagaacc agatgattgc cgttgtcgat gacgcaatcg
     4681 agctgggcgt cgaaatagga gcggcagcgc ccgcccgcct gccccgcggc ctcgaacagc
     4741 accacgcgcg cgcctttctc cgccagcgcc acggcggcgg aaagtccggc cagtcctcct
     4801 ccgacgacat agaccgtgct catgcgcgag gttcttttct tggtcgcgca atcatgcggc
     4861 gaaccggttc ccacttcgcc ggattgcgct ccaggccgtg acgcagcacg atcatcgcca
     4921 attgcgcctt ggggatgcgc acgcggcggc gcggcggcgc ccagccggcg gcttccatct
     4981 tgtgcaggat ttccgcatag accgctccca tcaggcgcgg tgcgcgcaga ttgccttccg
     5041 gtttggcggc gagaatgcgc ccggcctcct ggtaatggcc atgcgccagc ttggcgacct
     5101 cgcggcaggc cttgtcgatg gcgggatcgg cgagcgcctc gaacggctcg ccgatggtca
     5161 ccccggccgc ggcaaggaat tcgcgcggca gataaagccg atggatcgcg gcgtcctcgt
     5221 ccagatcgcg caggatgttg gtgagctgca gcgcacggcc gagatgatgg gcgagcgtga
     5281 aaccaggccc ctcttccatg ccgaaaacct tgatcgagag ccggccgacg gcgctcgcca
     5341 cgcggtcgca ataaaggtcg agggtggcga gaccgggagc gacgatgtct tccgtcacgt
     5401 ccatgtccat gccgtcgatg acggcgagga aatcctcggc gcgcggacgg tagcgcgcca
     5461 ccgtctcgga gagaaatgcc gcctggcccg gcgtgccgcc cgcgcagagc cgcttcacgt
     5521 cctcgcgcca cgcatcgagc gccgcatggc gttcggcgcg ggtgccgatg ccgtcgtcgg
     5581 cgatgtcgtc caccgcgcgg cagaaggcgt agatcgcgaa catcgcctcg cgctcggcct
     5641 tcggcatcag gcgcatggcc gaatagaacg agcttgtggc ggccttgcgg cgcaccgcct
     5701 cgatctccgg cgcggaaatt gcggactggg tcatggccgc tgcgttctag tgtcccgatt
     5761 ccgaaattcg cactatcttt atatcggctc cgggcgaatt tcggaatcga aaggacacta
     5821 gtaacctatt gaatcctagt gtcgttttgg tttcgaagtt cgcttgcagc cagatagtgc
     5881 ggatggtgcg aacttcgaaa ccacgacact aggacgaagc cgcgcgtcaa ggaagcgggc
     5941 aaatgccgac acagcaaacc ccggcaattc aagcttgcga tgatggacgc gctcggacaa
     6001 gggatcgcgg accataagct tggcgttgag gtcttccgcc agccgctgga tcaggctgac
     6061 ctccagggcg agacggtggt ctttgatatg ggccgcaaag ggggccgact gcgtcagcaa
     6121 tccggcgttg cggcgcgcca gagccgcgat caccgagcgc aaggccggcg tcgcgcgcgc
     6181 accggcgagc atgtcgggcg tggcgccgtg ttcggccatg atgtcgagcg gaagatagat
     6241 gcgatccagc gaacggtaat ctttcgcgca atcctgcaaa tgattgatga cctgaagcgc
     6301 ggcgcacagc gcgtcggaaa gcggccagac ggcgcggtcc tcgccatgca cgtcgagcac
     6361 gtagcggccg acgggcatcg ccgaatagcg gcaataatcc atcagctcgc tccaatcggc
     6421 gtagcgcagc ttggtgacgt cgcggcggaa ggcttccaat agatcggcgg cgtgatcggt
     6481 ggtcaagccg cgttcggcaa gcagcttgcg cagcgcggcg ccttccggcg aaaaatcgga
     6541 cgcgcccatc aggctgaggc gcatgctttc gagcagacgc agcttatcct cggcgctggc
     6601 gccggcgtga tcggcgacat cgtcggcggt gcgcacgaag cgatagaagg ccaggatcgc
     6661 cgggcgatgg cgcgcggcaa caaacaccga ggcgacgggg aaattctcgt cgcgatggcc
     6721 cttgcccgaa gagaacgctg ccgcctcgct catgccggtg tgatcgcctg ggcgcggccc
     6781 ttccacatgc cgccgcgccc gcgccagtgc tgcacggcgg aatcgatggt gaaggccgca
     6841 taaaagacgc cgatcgccgg cagcgccagc ccccacagag gagaacggcg atagaaatac
     6901 aacatcggct ggaaggcggc ggccattacc gcccaggcca gcgtgccgaa aaacggagcc
     6961 aaaccgccca tcgccagacc ggccagcggc acgacagtga gcaaagccat gccgagcacg
     7021 gttccggcaa ggacaagcgg cgaatagccg agctgggcgt aagccgagcg cgccaccatg
     7081 gcgcggatgt cggcgatgcg cggatagggc cgcaggctga ccgcgcgttc cgtcaggccg
     7141 agccagatcg ggccttgcgt cttcagccgc cgggcgagcg cgcaatcgtc gatgatctcg
     7201 gcgcggatgg cttcgatgcc gcccgcgcgc gccagcgctt cgcgcctgac cagcatgcag
     7261 cctccagccg cggcggcggc gtgttttttc ggatcgttga cccaggcgaa gggaaacagc
     7321 atggcgaaga agaagacgaa ggcgggaatc aggaaccgct cggcgaaact ttcgcagttg
     7381 agcttggcca tcagcgaggt cagaacgaat ttgtttgctt ccgcgcgggc caccagacgg
     7441 cgcagattgt ccggcgcgtg ggcgatatcg gcatcggtaa gccagatata gtcgggcgga
     7501 tcttccgcgt tcgcctgcgc cacgccctga tggacggcga acagtttgcc ggtccagccg
     7561 gcgggatggg ccgcgccgtc cagcaccgtc aggcgctcgc tgccgagcgc gcgcgcggcc
     7621 gcaccggtgc cgtcgccgct ctgatcgtcc accaggatga cgcggaagga gccgggataa
     7681 tcctgcgcca gaagactgcc ggcggaacgc gcgatcaaat cggcctcatt gcgcgcggga
     7741 accaccgccg ctaccgacgg ccaggtggcg ggttcaggcg gttcgttgcg gtcgtcgcgt
     7801 tcgcgcgcct gccagaaaaa gccgcggccg aacagcagcc ccagccagac cgccagcgac
     7861 agcagcgcca ggagcgaggc ggcgatcatg tcagatagcc cgccatgcgg aaccaggcga
     7921 gcgcgtcgat cagcgcctca tggtaaggcc gcgcgcggta gccaagctcc cgctcggcct
     7981 tggcggaggt gaaaaacatg cga
//
LOCUS       DASWPU010000151         8845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_13339,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000151 DASWPU010000000
VERSION     DASWPU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13339"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VGC27_03420"
     CDS             complement(<1..592)
                     /locus_tag="VGC27_03420"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0281657.1"
                     /translation="MIFDAKWGWAGDMTEPFDRSQANLNRSPLDDPRVLSGPIWGELR
                     VFLAVAKAKSFNRAAEELNMSQPTVSRAVKRLQDVIGSQLVVPTQSGIKLTVKGKELA
                     EWLLTLDEKLFEIAHDLKRETQEAEGLVRLSVTDGLAGLFVAPNLVQFSDQYPKIHLH
                     IRNPINLTSLRENQTDVMIGFSPTNQADITSRPLGYM"
     gene            944..1894
                     /gene="secF"
                     /locus_tag="VGC27_03425"
     CDS             944..1894
                     /gene="secF"
                     /locus_tag="VGC27_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY0281658.1"
                     /translation="MPLLRFIPEATKINFIGARYYAFAVDGLLLLVSIIAIAIYGFNM
                     GIDFKGGVSIQVKAEQVIDVGMVRSEVSSLNFRDQRIQNIGGGFCDKPVNSCVLIYVL
                     PKANQDGAAAKAEIQKKLGPAYTILSTNVIGPKVSGELFRSGIYATILAIIAIAIWVA
                     VRFEWQYGISAALATGHDVFVTAGLFSLMQLDFTLTSIAALLTLAGYSINDTVVVFDR
                     LRENRRKYKRMPLPDLINLSTNQILTRTILTSVATAVTIIPLLLFVPALFEFTGSILF
                     GIIVGTFSSTYVAAALLLYLPQPAGSISAPVPAPSGQPSE"
     gene            complement(1930..2496)
                     /locus_tag="VGC27_03430"
     CDS             complement(1930..2496)
                     /locus_tag="VGC27_03430"
                     /inference="COORDINATES: protein motif:HMM:NF018302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEY0281659.1"
                     /translation="MIRRKLILGAAAAAFAFATVPAFAEEDDATYTEREITRAAADFF
                     GFTTETVAKAVQRVFKDLGQPDGYIKGDEGSGAFVVGARYGSGWLVRKNHEPKKVYWK
                     GPSVGFDVGGNASKSFTLVYNLKREKRLYQRFPGVEGTYYFIAGIGVIYMRSGGVTLA
                     PMRTGVGLRAGVNAQYQVYSEKRDWFPL"
     gene            2677..2919
                     /locus_tag="VGC27_03435"
     CDS             2677..2919
                     /locus_tag="VGC27_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281660.1"
                     /translation="MNEPVTNRKLGPSGWVALIVLAGLLAASVWYAIHAWITLSGVAM
                     SPLGWLFMILGALVTLGLGAGLMALVFYSSRHDFDR"
     gene            complement(3322..4173)
                     /gene="cysQ"
                     /locus_tag="VGC27_03440"
     CDS             complement(3322..4173)
                     /gene="cysQ"
                     /locus_tag="VGC27_03440"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY0281661.1"
                     /translation="MTESIHAHSTSPMLRALATIVYEAGEIVLGYYTEEITARQKEDR
                     SPVTDADVAAERFILKRLAQLAPGIPVIAEEEVAAGRIPKIGHKFFLVDPLDGTKEFI
                     NRNGEFTINIAEINDGKPVRGVIYAPAKDRLFFGEVLAGAFEIFTPPGGAPDFAEARQ
                     IFARAPAPDGLVAAISRSHNDKKTEAYLAAYKIKEFLVGGSSLKFCQVAAGEADIYPR
                     HGRTMEWDTAAGHAILAAAGGTLTTPEGKPFVYGKTEAKFANPHFIARGLAEGAAAGR
                     PDSDKPL"
     gene            4386..5006
                     /locus_tag="VGC27_03445"
     CDS             4386..5006
                     /locus_tag="VGC27_03445"
                     /inference="COORDINATES: protein motif:HMM:NF021595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEY0281662.1"
                     /translation="MNDLEFSAFLVSRVCHDLVGPLGAVVNGLEVLEDERDAAMRADA
                     LRIVTSSATQALARLQFLRIAFGAAGSAGAELDLGEVSRLVAGLFSGGKIALHWETPS
                     VNWPKDWAKLVMNATVIAADCLPRGGSVRIETSTDPSVFGFTIVAAGQNARIVAEVER
                     ALRGEAAEEPYDARGVQPYLTHRLAKKLNAGLTMTAREGQVELVAG"
     gene            5100..5465
                     /locus_tag="VGC27_03450"
     CDS             5100..5465
                     /locus_tag="VGC27_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0281663.1"
                     /translation="MKTCLVVDDSRVIRKVACRILAALSFETEEAADGASALTVCRRK
                     MPDAILLDWQMPNMSGIEFLRTLRSERDGANPVVVFCTTENDVACIAEALNAGADEYI
                     VKPFDRSIVEAKFAEVGLV"
     gene            5480..6322
                     /locus_tag="VGC27_03455"
     CDS             5480..6322
                     /locus_tag="VGC27_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEY0281664.1"
                     /translation="MNQKDFAFLAELLHRRSGLVLTQQKVHLVEGRLAPVMSRFGFKD
                     NAALVRELRHGNEVLAGAVTEAMTTNDSAFFRDRRTFEEFRNIVLPELLIRRARTKCL
                     RIWCAACAAGQEPYSIAMIVEDANLIAQGWTVDLIASDLNSQMIERAEEGLYSQFEVQ
                     RGLPIRRLVRNFAQEGGNWRISESLRRMVTFRTFNLLDSFGWLGEMDVVFCRNVLMYF
                     DRKTRISVIGRIAGVLAADGALLIGPAESVTGLAPGFIQPEQAPGLYYRTSYSPQMLR
                     RKAG"
     gene            complement(6354..8285)
                     /locus_tag="VGC27_03460"
     CDS             complement(6354..8285)
                     /locus_tag="VGC27_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0281665.1"
                     /translation="MRKSSLLTSAALFALLAPGFAIAADAPETVVVTATRTSQPVEKT
                     GASIAIITGGDLQIQQLNVLSDALQQTPGVTVNRTGGVGQVTTVSLRGAEAGQTVALI
                     DGIRINDPSDVSEGAMFGDLLVNNIDRVEVLRGPQSTLYGSDAIGGIVNVITKRGGPF
                     ALTASAEGGSFNTYRLNLAASGTQSIAEYGLAINAFGTKGVSAADSRNGNPEADGYRN
                     LGATFNTRFHLSDDVSVDLRGYYTRGHSGFDDNYAYTPPYQLEDSDAKNTNQLYAGYA
                     GLNGDLFGGRFHNRLALIATSSTRQFFDSASDTLHLNYEYSGDAVRFEYQGTVDIGAD
                     SQATFGAESEQRSFRNNNYYSYMPADSAAGHDRITGIYGQIQSTLFDQLTLTGGVRYD
                     DDKEFGSHTSVKLAAAWQVPVLDATLRANYGDGFKAPSLYQLYSPYSNPIAALKPETA
                     RGWEVGADKDLWDGRVRASLTYFERRTKNQIDFQSCYTPADAPGCPQRLAQYGYYINL
                     GRTRATGVEAAIVAEITDTLKLAANYTNLSAVNTGTGTDLARRPRDTANATVTWLALQ
                     DLTLGASIRYLGKRFNDAGNFTPLTSNTKIDLFGAYDLAGNWQLFGRVDNLLNDRTEY
                     VSGYGTPGLAATFGVRTRL"
     regulatory      complement(8440..8608)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         1554 a         2731 c         2787 g         1773 t
ORIGIN      
        1 gcatgtaccc gagagggcgt gacgtaatat cggcttggtt cgtcggcgaa aatccaatca
       61 tcacgtcggt ctgattttcg cgaaggcttg tcagattgat cgggtttctg atgtgcaggt
      121 gtatcttggg atattggtcg ctgaactgaa cgagattggg tgcgacaaac aggcccgcca
      181 acccatccgt gacgctcaac cgcacgagtc cctcggcctc ttgcgtctcg cgcttcaaat
      241 cgtgggcaat ctcgaacagc ttttcgtcga gcgtgagcag ccactccgcc agttccttgc
      301 cttttaccgt cagtttgatc ccgctctggg tcggcaccac gagctgcgag ccgatgacgt
      361 cctggagccg cttgaccgcg cggctgaccg tcggctggct catgttcagt tcctcggcgg
      421 cgcgattgaa ggatttcgcc ttcgcgacgg caagaaacac ccgcagttct ccccagatgg
      481 gcccgctcag gacacggggg tcgtcgagcg gcgaacggtt gaggttcgcc tgcgaccgat
      541 caaatggctc cgtcatgtcc ccagcccagc cccacttggc atcgaagatc atcacaactc
      601 agaatatgag aatcatctca tagaacttgg ggctgcgcca tcaaacgaat tcgctacggc
      661 cagcgcgcaa aggctttttt gacgcaacgg cacaccagag gaaatacaac tatagataga
      721 ataacctacc attcccggct tggcggtggg gtctgcaccg cgagggagaa cggaatccgc
      781 agacccgggc gaagatgcgg attttcctct ctcgcgttca cgacggccgg gaacggcaaa
      841 gggcgcttca actcaaggcg gtgatatcgc tgcgccgtgc gatgcttgtc ccaggcgttc
      901 ttaaccgcta tgtctgcgcc cgcccaattg gaccattaag ccaatgcctc tgctgcgatt
      961 tatcccggaa gcaaccaaaa tcaacttcat cggcgcgcgc tattacgcct tcgccgtgga
     1021 cggactgctg ctgctggtct cgatcattgc gatcgcgatc tatggcttca acatgggcat
     1081 cgatttcaag ggcggcgtgt cgatacaggt caaggcggag caggtgatcg acgtcggcat
     1141 ggttcgcagc gaggtttcca gcctcaactt ccgcgatcag aggattcaga atatcggcgg
     1201 cggcttctgt gacaagccgg tcaattcctg cgtgctgatt tatgtgctgc ccaaggcgaa
     1261 ccaggacggc gccgcggcga aggccgaaat ccagaaaaaa ctcggccccg cctacacgat
     1321 tctgagtacc aacgtgatcg gccccaaggt cagcggcgaa ctgttccgca gcggcattta
     1381 tgcgacgatc ctcgccatca tcgccatcgc catctgggtg gcggtgcgct tcgagtggca
     1441 atacggcatc agcgcggcgc tcgccaccgg ccacgacgtg ttcgtgacgg cgggcctgtt
     1501 ctcgctgatg caactcgact tcacgctgac cagcatcgcg gcgctgctga cgctggcggg
     1561 ctattcgatc aacgacacgg tggtggtgtt cgaccggctg cgcgagaacc ggcgcaaata
     1621 caagcgcatg ccgcttcccg atctgatcaa tctatcgacc aatcagatac tgacgcggac
     1681 gatcctcacc tcggtcgcga ccgcggtcac gatcatcccc ttgctgctgt tcgttccggc
     1741 gctgttcgaa ttcaccggct cgatcctgtt cggcatcatc gtcggcacct tctcgtccac
     1801 ctatgtggcg gcggcgcttc tgctctatct gccccagccg gccggctcca tcagcgcgcc
     1861 cgtgccggcg ccaagcggac agccgagcga ataggaactc tattcgctac tcgctattcg
     1921 cttgttcttc tacagcggga accagtcgcg cttctcggaa tagacttggt attgcgcgtt
     1981 gacgcccgcg cgcaggccca cgccggtgcg catcggcgcc agcgtcacgc cgcccgagcg
     2041 catgtagatc acgccgatgc cggcgatgaa gtaataggtg ccttcgacgc cggggaagcg
     2101 ctgatacagc cgcttttccc gcttcagatt gtagaccagg gtgaagctct tcgaggcgtt
     2161 gccgccgaca tcgaagccta ccgacgggcc tttccaatag accttcttcg gctcgtggtt
     2221 cttgcggacc agccagcctg agccgtagcg cgcgccgacg acgaacgcgc ccgaaccctc
     2281 gtcgcccttg atgtagccgt cgggctggcc caggtccttg aacacgcgct gcaccgcctt
     2341 ggcgacggtt tccgtcgtga agccgaagaa atcggcggcg gcgcgggtga tctcccgttc
     2401 ggtgtaggtg gcatcgtctt cttcggcaaa ggccggaacc gtggcgaacg cgaaggccgc
     2461 ggcagccgcg ccgaggatca atttacgcct gatcatgaac cgctactcct ctcgaatcac
     2521 acgtcccctg taccgggcag catcgcccaa aaaacgcacc ggcgcgagat ggctcccccg
     2581 ccaggataaa aggaaatatg gcgacaacat gatgttgccg ccttctatcc ttgggcccgg
     2641 cgcttcgtta caacctgcgc taggggaatt ttcttcatga acgagcccgt aacaaacagg
     2701 aaacttgggc cgtccggctg ggtggcgctg atcgtgctgg cgggcttgct cgcggcatcg
     2761 gtgtggtacg cgatccatgc ctggataacg ctgtccggcg tggcgatgag cccgctgggc
     2821 tggctgttca tgattttggg cgcactggtc acgctcggcc tcggcgccgg gctgatggcg
     2881 ctcgtcttct actccagccg ccacgatttt gaccggtgat cctggcggac gcgaagcagg
     2941 aaaagcagaa caaggttcag cccttttttc ccagctccat tttgtcatgg cctgctccct
     3001 tcgacacgcc aagccaccct caccctgagc ttgtcgaagg gtgtggggca attccgtctg
     3061 aatgcatttt gctctaggcg tccattctcc caactcaagt gcataatcgc ccttgaatct
     3121 ctggattcta ttcccacatc cgcactcggt ttggtccagc cgttttgggc gcactgaaaa
     3181 ggaattagag agggggatac ccccctcgaa tctcgaaaac gaatggaccg gaaaggaaag
     3241 cgcaagaaat cggaaatgag ccgggcaggg ggcacccccc atgaaaaact cgaaaacaac
     3301 ctcggccgca atttcgcgcg attacagcgg cttgtcggaa tccggccggc ccgcggcggc
     3361 gccttccgcc agcccgcgtg cgatgaaatg cggattggcg aacttcgcct cggtcttgcc
     3421 atagacgaac ggcttgcctt ccggggtcgt cagcgtgccg cccgccgccg cgaggatggc
     3481 gtggccggcc gccgtatccc attccatggt gcgtccgtgg cgcggataga tgtcggcttc
     3541 acccgccgcc acctgacaga atttcagcga cgagccgccc accaggaatt ccttgatctt
     3601 gtaggcggcg aggtacgctt cggttttctt gtcgttgtgc gagcggctga tggcggcgac
     3661 gaggccgtcc ggcgcgggcg cccgggcgaa gatttggcgg gcctcggcga agtcgggcgc
     3721 gccgcccggc ggggtgaaga tttcgaaggc gcccgccagc acttcgccga agaacagccg
     3781 gtctttggcc ggcgcataga tcacgccgcg caccggcttg ccgtcgttga tctcggcgat
     3841 gttgatggtg aactcgccgt tgcggttgat gaattccttg gtgccgtcca agggatcgac
     3901 caggaagaat ttgtggccga tcttgggaat gcgcccggcc gcgacttctt cctcggcgat
     3961 gacgggaatg cccggcgcga gctgcgccag ccgcttcagg atgaagcgtt cggcggccac
     4021 gtccgcgtcg gtcaccggcg agcggtcttc cttctgccgc gccgtgatct cttcggtgta
     4081 atatccgagc acgatctcgc ccgcttcgta gacgatcgtc gccagggcgc gcagcatggg
     4141 cgaagtcgag tgggcgtgga tgctctcggt catggttgtc ggcgcggcct ggccgccggc
     4201 tccggattcg ccgggtcagt atgcacgagg cgcttgcccg cgtattccaa atcgagcgtc
     4261 atgcgatctt gcgccattcc ctggtccggc gtcggcattg tacctatctt aaacattcct
     4321 taagaggcga gtcagacgat aaccatacgt ccgctgttgg tcacagcccc ggagacctga
     4381 accccatgaa cgatctcgaa ttttccgcgt ttttagtcag ccgggtctgc cacgatctgg
     4441 tggggccgct gggggcggtg gttaacggcc ttgaggttct ggaggacgag cgtgatgccg
     4501 ccatgcgcgc ggatgcgctc aggatcgtga cctccagcgc gacccaggcg ctggcgcggc
     4561 tgcaattcct gcggatcgcc ttcggggcgg ccggctcggc cggtgcggag ctggacctgg
     4621 gcgaggtcag ccgtctggtc gccggattgt tcagcggcgg caagatcgcg ctgcactggg
     4681 aaacgccgag cgtgaactgg cccaaggatt gggcgaaact ggtgatgaac gccacggtca
     4741 tcgcggccga ttgcctgccg cgcggcggct cggtgcggat cgaaaccagc acggatccgt
     4801 cggtcttcgg cttcaccatc gttgccgccg gccagaatgc acgcatcgtc gcggaggtcg
     4861 agcgggcgct gcgcggcgaa gcggccgagg agccgtacga cgcgcgcggc gtacagcctt
     4921 atctgaccca ccggctcgcc aagaagctga atgccgggct gacgatgacc gcgcgcgaag
     4981 gccaagtgga gctggttgct ggttaattgc ccttcggccg cgtacatcgg acatttactt
     5041 tcattgcgta ggcttgggca cgaacaattg gattcgggcc cgtgcctctg gggttcgcca
     5101 tgaaaacttg tcttgtcgtc gacgacagcc gtgtcattcg caaagtggca tgccgcattc
     5161 tcgcggccct gtcgttcgag accgaagagg cggccgatgg cgcgtccgcg ctgacggtct
     5221 gccgccgcaa gatgcccgac gcgattctgc tcgactggca gatgccgaac atgagcggca
     5281 tcgagttcct gcgcactttg cgcagcgagc gcgatggggc caatcccgtt gtggtcttct
     5341 gcaccaccga gaacgatgtg gcttgcatcg ccgaggcgct gaacgccggt gccgacgagt
     5401 acatcgtgaa accgttcgac cgcagcatcg tcgaagcgaa gttcgccgaa gtgggtttgg
     5461 tgtaagggtt aagggggcta tgaatcagaa ggatttcgca tttctcgcgg aactgctgca
     5521 taggcgctcg gggctggtgc tgacccaaca gaaggtgcat ctggtcgaag gccggctggc
     5581 gcccgtcatg tcccgtttcg gcttcaagga caacgccgct ttggtgcgcg agctgcgtca
     5641 cggcaacgag gtgctggccg gcgccgtgac cgaagccatg accaccaacg attccgcctt
     5701 cttccgcgac cgcaggacgt tcgaggaatt ccgcaacatc gtgctgccgg aacttctgat
     5761 ccgccgcgcc cgcaccaagt gcctgcgcat ctggtgcgcg gcctgcgcgg ccggccagga
     5821 gccttattcg attgcgatga tcgtggagga tgccaatctt atcgctcagg gctggaccgt
     5881 cgacctcatc gccagcgatc tcaactcgca gatgatcgaa cgtgccgagg agggcctcta
     5941 ttcgcagttc gaagtgcagc gcgggctgcc gatccgccgg ctggtgcgca atttcgccca
     6001 ggaaggcggc aattggcgca tttcggaaag cctgcgccgc atggtgacgt tccgtacctt
     6061 caatctgctc gattccttcg gctggcttgg ggagatggat gtcgtgttct gccgcaacgt
     6121 gctgatgtat ttcgaccgca agactcgcat ttccgtgatt ggcaggatcg ccggcgttct
     6181 cgcggccgac ggcgcgctgt tgatcggccc ggcggaatcg gtcaccggcc tggcgcccgg
     6241 cttcatacag cccgagcagg cgcccggtct ctattaccgc accagctact cgccgcagat
     6301 gcttcgccgc aaagcggggt agacggcggc aatcctgccg ccgccttctt ccgctacagc
     6361 ctggtgcgca cgccgaacgt cgcggcgagt ccgggggttc cataacccga gacatattcg
     6421 gtgcggtcgt tgagcagatt gtcgacgcgc ccgaatagct gccaattccc ggcgagatca
     6481 taggcgccga acagatcgat tttcgtgttg ctcgtcagcg gcgtaaaatt gcccgcgtcg
     6541 ttgaagcgct tgcccaggta gcggatgctt gcgccgagcg taaggtcttg cagggcgagc
     6601 caagtgacgg tcgcgttcgc cgtatcgcgc ggccgccgcg ccaagtccgt tccggtgccc
     6661 gtgttgaccg ccgacaggtt ggtgtagttc gcggcaagct tcagcgtgtc ggtgatctcc
     6721 gcgacgatgg cggcttccac gcccgtcgcg cgcgtgcggc cgaggttgat gtaatagccg
     6781 tactgggcaa gccgctgcgg acatcccggc gcgtcggcgg gggtgtagca gctctggaaa
     6841 tcgatctggt ttttcgtcct gcgctcgaag taggtgagcg aagcgcgcac gcgtccatcc
     6901 cacaggtctt tatcggcgcc aacttcccaa cccctggccg tctccggttt caatgcggcg
     6961 atcgggttgg aatacggcga atagagctgg tacagcgacg gcgccttgaa gccgtcgccg
     7021 taatttgcgc gcagggtcgc gtccagaacg ggcacctgcc aggccgccgc cagcttgacg
     7081 gaagtatgcg acccgaattc cttgtcgtcg tcgtagcgca cgccgccggt cagcgtcagt
     7141 tggtcgaaga gcgtgctctg gatttgccca tagatgccgg tgatgcggtc gtgtccggcg
     7201 gcggaatcgg cgggcatata ggaatagtag ttgttgttgc ggaacgagcg ctgttcgctt
     7261 tccgcgccga aggtcgcctg gctgtccgcg ccgatatcca cggtgccctg atattcgaag
     7321 cgcacggcgt cgccgctgta ttcgtagttc aggtggagcg tatcggatgc ggaatcgaag
     7381 aactgccgcg tactcgaggt ggcgatcagc gcaaggcggt tgtggaagcg tccgccgaac
     7441 agatcgccgt tcagccccgc atagcccgcg taaagctgat tggtgttttt ggcgtcggaa
     7501 tcttcgagtt ggtagggcgg cgtgtaagcg taattgtcgt cgaagcccga atgcccgcgt
     7561 gtgtaatagc cgcgcagatc gacgctgaca tcgtcgctca gatggaaccg cgtattgaac
     7621 gtcgcgccca gattgcgata gccgtccgct tcgggattgc cgtttcgcga atccgccgcg
     7681 gacacgcctt tggtgccgaa ggcgttaatg gcgaggccgt attccgcgat gctttgcgtc
     7741 ccgctggcgg cgaggttcag gcgataggtg ttgaaagaac cgccttcggc gctggccgtc
     7801 aacgcaaagg ggccgccgcg cttggtgatg acgttcacga tgcccccgat ggcgtcgctg
     7861 ccgtaaagcg tcgattgggg accgcgcaaa acttccaccc ggtcgatgtt gttgaccagc
     7921 aaatcgccga acatcgcgcc ttccgacacg tcgctcggat cgttgatgcg gatgccgtct
     7981 atcagcgcca cggtctggcc cgcttccgct ccgcgcagac tgacggtcgt cacctgtccg
     8041 acgccgccgg tgcgattgac cgtcaccccc ggcgtttgct gcaacgcgtc gctcaacacg
     8101 ttgagctgct ggatttgcag atcgccgccg gtaatgatcg caatcgacgc gccggttttc
     8161 tccacgggct gagatgtgcg cgttgccgtt accaccacgg tctccggcgc gtctgcggca
     8221 atagcgaagc cgggcgcgag gagcgcgaac agggcggcag atgtaagcag agatgatttg
     8281 cgcatgacgt tcccctgggt tgaaccagga gccggcacgc ggaagcgcca ttcgcgattt
     8341 gctgaacgaa ttctggcgaa caccaagggc cccgcggcga cgactccaca ctgtcgtcgc
     8401 gacggtttcc cccgcagtcc tcggcgcacc ccggccgaaa acaggacgac ggcgtggggc
     8461 aggtttcctg gcttccggtt ctttcgttcg cgcccgcctt cccaaggctt cgcctcagtg
     8521 gcagttggac gctttctcgc cggtcacagt tgcgggggca gccgcggtat cgcaccgcgt
     8581 tccctaaacc tcacgcgtgg gaacagttaa cctagccgcc gcgtcggcgt caatctgctg
     8641 tcgcggatcg gctgcaaacc gcgcataaaa tgccgctacg cgaatcggaa cgggcacgcg
     8701 cgacatgaca aggaaattca aattctgggc ggggttaagc cggagcttat gggccgcggc
     8761 cgcgggcgcg ctcgtgggtg ccggggccgt tgcttggctc aacgacacgg gcgcgtggct
     8821 tgtactgctg gccgccgccg ccgcc
//
LOCUS       DASWPU010000152         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_111380,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000152 DASWPU010000000
VERSION     DASWPU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111380"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VGC27_03465"
     CDS             complement(<1..476)
                     /locus_tag="VGC27_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY0281666.1"
                     /translation="MNPIKDDIRPEQLASALGERYLAYALSTITQRALPDARDGLKPV
                     HRRLLHAMRLLGLNPAAGFKKCARVVGDVIGKYHPHGDQAVYEALVRLAQDFAVRYPL
                     VEGQGNFGNVDGDNAAAMRYTEARLTDAAQALLEGLDENAVDFRNTYHGVEEEPVVL"
     gene            complement(584..1330)
                     /gene="recO"
                     /locus_tag="VGC27_03470"
     CDS             complement(584..1330)
                     /gene="recO"
                     /locus_tag="VGC27_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY0281667.1"
                     /translation="MEWSDDAIVLSVRPHGESSAILEALTRTHGRHLGLVRGGASRRT
                     RPVLQPGNSVRAQWRARLSEHLGSFTVEPVRARAGDLMEGREKLVGLNALTAIAGAAL
                     PERESHAPVFEVANILLDAMMEDGLAHWGPLYVRWEAGLLEALGFGLDLSHCAATGTM
                     ENLRFVSPRSGRAVSGEAGAPYAGRLFALPAFLLGSQNGEVAPGELAAGLALTGHFLL
                     ERVLRPQGKDIPGARLRLDALAAHEDSESR"
     gene            complement(1492..>1984)
                     /locus_tag="VGC27_03475"
     CDS             complement(1492..>1984)
                     /locus_tag="VGC27_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY0281668.1"
                     /translation="VEKLNAGVFERVFMISALKGDGLNDLGDWCASKMPKGPWLYPAD
                     QYADIPSRLLAAEITREKIYLRLHDELPYASAVETEKWEERKDGSVKIDQIVYVQRDG
                     QKAIVLGKGGATIKIIGELARKEMEEVFARRVHLFLFVKVREDWADKREHYREIGLEF
                     PEE"
BASE COUNT          376 a          656 c          599 g          353 t
ORIGIN      
        1 agtacgacag gctcttcctc gacgccgtgg taagtgtttc ggaaatcgac ggcgttttcg
       61 tccagccctt cgagcagcgc ctgcgccgcg tcggtcagcc gcgcttcggt gtagcgcatg
      121 gcggcggcgt tatcgccgtc cacattgccg aaattgccct ggccttccac cagcgggtag
      181 cgaaccgcga aatcctgcgc caagcgcacc agcgcttcgt acaccgcctg gtcgccatgc
      241 ggatggtatt tgccgatgac gtcgccgacg acgcgggcgc atttcttaaa ccccgccgcg
      301 ggattgaggc caagcaggcg catggcatgc agcagccggc gatggacggg cttgaggccg
      361 tcgcgggcat cgggcagtgc gcgttgggtg atggtggaca gcgcataagc gagataacgc
      421 tcgccgagcg cgctggccaa ttgttcggga cggatatcgt ctttgatcgg gttcattggg
      481 gactcaagtc atgtcgccct cacccttcga caagctcagg gtgaagggct tggctaatcc
      541 ctcatgctga gcttgtcgaa gcatgaagga gacgaaacaa cgtctaccgc gattcgctat
      601 cttcatgtgc cgctagagca tcgagacgta acctcgcgcc cggtatgtct tttccctgcg
      661 gacgcagtac gcgctccaag agaaaatggc cggtcagcgc cagtcctgcc gccagttcgc
      721 cgggagcgac ctccccattc tgcgaaccga gcaggaatgc tggcagtgca aacagacgcc
      781 cggcataagg cgcgcccgcc tcgcccgaca ccgcccggcc ggaacgcggc gagacgaagc
      841 gcaggttttc catcgtgccc gtcgcagcgc aatgcgagag atcgaggccg aaacccaacg
      901 cctccagcag cccggcctcc cagcgcacgt atagcggccc ccaatgcgcc agcccatcct
      961 ccatcatggc gtcaagcagg atgttggcca cctcgaacac cggcgcgtgt gattcgcgtt
     1021 cgggcagcgc cgcaccggca atggcggtga gcgcgttgag gccgacaagc ttttcacggc
     1081 cttccatcaa atcgccggca cgggcacgaa caggctccac cgtgaagctg cccagatgct
     1141 cggagagccg cgcccgccat tgcgcgcgca cggagttgcc cggctggagc accggcctgg
     1201 tccgccgcga ggcaccgccg cgcaccaatc ccagatggcg gccgtgcgtg cgggtcaagg
     1261 cttcaaggat cgcactgctt tcgccatgcg gacggacgga caggacaatg gcatcgtcag
     1321 accattccac cttagaaacc caaaacaaaa aacgtcatgg ccggcccccg agccggccac
     1381 ccatcctagt gatggcgtca ggacggcgcg cgttcatgcg ccagaagatt ctggtgtttc
     1441 gctggatggc cgggtcaagc ccggccatga cgaaataaga gcgaggcaaa cttattcctc
     1501 cggaaattcc agtccgattt cgcggtagtg ctcgcgcttg tcggcccagt cctcgcgcac
     1561 cttgacgaac aggaagagat gcacgcggcg ggcgaagacc tcttccatct ccttgcgcgc
     1621 cagctcgccg atgatcttga tcgtcgcccc gcccttgccg agcacaatcg ccttctggcc
     1681 gtcgcgctgg acatagacga tctgatcgat cttcaccgag ccgtccttgc gctcctccca
     1741 tttctcggtt tcgaccgccg aggcgtaggg cagctcgtca tgcaggcgca gatagatttt
     1801 ctcgcgggtg atttccgccg ccaagaggcg cgagggaata tcggcgtact ggtcggcggg
     1861 ataaagccac ggtcctttcg gcatcttcga ggcgcaccaa tcgccgagat cgtttagccc
     1921 gtcgcccttg agcgcggaga tcatgaacac ccgctcgaac acgcccgcgt tcagcttctc
     1981 gacc
//
LOCUS       DASWPU010000153         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_56258,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000153 DASWPU010000000
VERSION     DASWPU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56258"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1139)
                     /locus_tag="VGC27_03480"
     CDS             complement(138..1139)
                     /locus_tag="VGC27_03480"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HEY0281669.1"
                     /translation="MKRISAVSAALAAALCVAAASAAGESASEKGAFGILFENDLFGN
                     ADHDYSNGVELTYTTAPQDTPDWLIAVAHWLPFFSATGKGDVRTRYALGQSMFTPIDK
                     TLPNPSLDDRPYAGFLFGTAGIVGDSGRHLDQLQFTLGVIGPMALAGEAQNWVHGIVG
                     DAKAQGWHYQLRNEPGLIIQYERSIKLIPPKSILGLIFDLEPHYGAAVGNVYDFANLG
                     VVARLGFNLPGDYGPMRIDPSLPGSNFFEPSGAIGAYVFAGVDGRAIARNLFLDGNTF
                     KTSRSVSKLNLVYDYDLGAAITLYAMRISYTYVIRSREYKTQPDVSKFGAIAVSFRF"
     gene            complement(1269..1826)
                     /locus_tag="VGC27_03485"
     CDS             complement(1269..1826)
                     /locus_tag="VGC27_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEY0281670.1"
                     /translation="MNIRGILLATSLATAVMLPIGLVDRASAASEVDLNKDSAQALQT
                     LYKLHPFAERLSREAKAVLVFPNVVKAGLIFGGAYGEGELLQGSKVDGYYNSVTGSWG
                     LQAGAQSYSYVVFLMSPKALRYIRESNGWEIGVGPTVVIVDQGKAKNLSTSTLKDDAY
                     AFIFGQQGLMAGISLEGTKISRIKR"
     gene            complement(2034..2432)
                     /locus_tag="VGC27_03490"
     CDS             complement(2034..2432)
                     /locus_tag="VGC27_03490"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0281671.1"
                     /translation="MQLDLPPRDRRSVDAYLSRPPRKRTCLNGKLVFSEDASFFNGSL
                     TLDCMIRDISEGGAKIVLSQHQLMPGGLYLIVVKYGVAHWAKVVWLEFPARGLKFITT
                     FKLREPLREELMFLRKLWDELYTRPGAVEW"
     gene            complement(2446..>3116)
                     /locus_tag="VGC27_03495"
     CDS             complement(2446..>3116)
                     /locus_tag="VGC27_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0281672.1"
                     /translation="KLFVPALENLGLILPVGAWVIDMACRQLGEWRTSGLGHIRIAVN
                     VSSRQVCEERFVPQVADAVRKHHVDPTLLEFEITESTLMAHGEAMNVTLRKLKNMGIT
                     ISIDDFGTGYSNLAFLKRFQVDTIKIDVSFIRDITTNADDAAIAIAIINMAHSLRLKV
                     VAEGVETREQLDFLRIYGCDEVQGYLLSKPVSATEISEKLRRTAVADFAALATLQNGG
                     QAYG"
BASE COUNT          658 a          889 c          914 g          655 t
ORIGIN      
        1 tgcatctcta tatcggcgtc gggcgaattt cggaatcgaa aggacactag caacctattg
       61 aatcctagtg tcgttttggt ttcgaagttc gcttgcagcc cgatataacg aatggtgcga
      121 acttcgaaac cacgacacta gaaccggaac gaaacggcaa ttgcgccgaa tttgcttacg
      181 tccggttgcg ttttgtattc gcggctgcgg atgacatagg tgtaggagat ccgcatcgcg
      241 tagagggtga tcgccgcgcc gagatcgtaa tcgtaaacca gattcaattt gctcacgctg
      301 cggcttgtct tgaaggtgtt gccgtcgaga aacagattgc gcgcgatggc gcggccgtcg
      361 actccggcaa aaacatacgc gccgatcgcc ccggacggct cgaagaaatt cgaaccgggc
      421 aggctcggat cgatccgcat cgggccgtag tcgcccggca ggttgaagcc caaacgcgcc
      481 acgacgccga gattggcaaa gtcgtagaca ttgccgaccg cggcgccgta atgcggttca
      541 aggtcgaaaa tcaggcccag aatggatttg ggcggaataa gcttgatgct gcgctcatat
      601 tgaatgatga gcccgggctc gttgcgcaat tgatagtgcc agccttgcgc tttggcatcg
      661 ccgacgatcc catgcaccca attctgcgcc tctccggcaa gcgccatcgg cccgattacg
      721 cccagcgtga actgaagctg atccagatgc ctgccgctgt cgccgacaat cccggcggtg
      781 ccgaatagaa atccggcata ggggcgatcg tccagcgacg gattcggcaa tgttttgtcg
      841 atcggcgtga acatgctctg gccgagcgca tagcgcgtgc gcacgtcgcc ttttcccgtc
      901 gcggagaaga agggcagcca gtgcgcgacg gcgatcaacc agtcgggtgt atcctgcggg
      961 gccgtcgtgt aggtgagttc tacgccattg ctatagtcgt gatcggcatt gccgaacaaa
     1021 tcgttttcaa aaagtatgcc gaaggcgcct ttttcagatg cggactcgcc cgctgcagac
     1081 gcggcggcga cgcacaatgc cgcggcaagc gccgcggaaa cagcagatat tcgcttcatg
     1141 gtatatcccc ctgacagagg agaggcagca gcggcaccgt atcctctcct tactcatgaa
     1201 ccatcgcgac gaacaaggcg gccctcatcc atcgcggatg gggccgccca agctcgtcgc
     1261 aggtttgttc agcgcttgat tctggagatt ttggttcctt caaggctgat accggccatc
     1321 aggccttgct ggccgaagat gaaagcgtag gcgtcatcct tcagcgttga ggtggacagg
     1381 ttcttcgctt tgccctgatc cacgataacg actgtcgggc ccacgccgat ttcccatcca
     1441 ttcgattcgc gaatgtaccg cagggctttg ggggacatca gaaacacgac gtatgaatac
     1501 gattgggcgc ccgcttgcag accccaggag ccggtcaccg aattgtagta accgtcgacc
     1561 ttcgatcctt gcagaagctc gccttcgcca tacgctccgc cgaaaataag gccggctttg
     1621 acgacgttgg ggaaaacgag aacggctttc gcttcgcgcg aaagcctttc cgcgaaggga
     1681 tgcagtttat aaagcgtctg caacgcctga gcggagtcct tgttgagatc tacctccgat
     1741 gccgcgcttg cgcggtcaac cagtccgatt ggcagcatga ctgccgtggc gagcgaggtc
     1801 gccaggagaa taccgcggat gttcatcgtt atacgttcct attgtgagag tggcgcaagc
     1861 acctttcatg aaggccgctt gcttctgggt ttgggaaaaa cgttgcggcg tgtacctgcc
     1921 aggacacgtt agtcctagtt tcgttccgca tgccgccacg tgaggctttg gccgccgggc
     1981 tgcgtaggct cggaatctac tcagacggcg ggatcgaagg gcacgaggcc ggcttaccat
     2041 tcgacagccc cgggccgggt gtagagttca tcccacagct tcctcagaaa catcaattcc
     2101 tcgcgcaggg gttcgcgcag cttaaatgtc gtgatgaact tgagcccgcg cgcgggaaac
     2161 tccagccata ccaccttggc ccagtgggca acgccgtact tgacgacgat cagatacaga
     2221 ccgcccggca taagctggtg ctgggagaga acaatcttgg cgccgccctc ggagatatcg
     2281 cgaatcatgc aatcgagcgt gaggctgcca ttgaaaaagg acgcatcttc gctgaacacc
     2341 aatttgccgt tcaagcaggt gcgtttccgc ggcggcctgg atagatacgc atccacgctg
     2401 cgccggtcgc gcggcggcag atccagctgc atcggcttcg tattattatc cataggcttg
     2461 acccccattc tgcaacgtcg ccagggctgc aaagtcggca accgcggttc gacggagctt
     2521 ctccgaaatc tcggtcgccg agacgggttt cgataggaga tagccctgaa cctcgtcgca
     2581 accgtagatg cgcagaaaat caagctgctc gcgcgtctcg actccttcgg cgacgacctt
     2641 gagccgcaga ctgtgcgcca tgttgatgat cgcgattgcg atcgcggcat cgtccgcgtt
     2701 ggtcgtgatg tcgcggatga aggaaacatc gatcttgatg gtatcgactt gaaagcgttt
     2761 cagaaaggcc aggttggaat aacccgttcc gaaatcgtcg atggagatcg taattcccat
     2821 gtttttcagc ttgcgcaggg tgacgttcat ggcttcgccg tgcgccataa gcgtgctttc
     2881 cgtgatctcg aattccagca acgttggatc gacgtgatgt ttgcgcacgg catcggcgac
     2941 ttgggggacg aaccgctctt cgcacacctg tcgcgatgac acattcacgg caatccggat
     3001 gtgaccgagc ccggacgtcc gccattcgcc gagctgccgg caggccatgt cgatgaccca
     3061 tgcgccgacc ggcaggatca atcccagatt ctcgagagcg ggtacgaaaa gtttcg
//
LOCUS       DASWPU010000154         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_68684,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000154 DASWPU010000000
VERSION     DASWPU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68684"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="VGC27_03500"
     CDS             <1..1593
                     /locus_tag="VGC27_03500"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY0281673.1"
                     /translation="IELEVNAEGRSNWTFAKEDKSTGSGGSKITLPANTEFSGIKIND
                     GSIFYTNDKTGTRRAIEHVNAAIAITRLDQPVAIDGNLTRNGHRVDFDVKVITPETLL
                     GNRTTMLDLSLTSDMMQAGFKGGLSQDGALAGTLKFDTASLRGVLGWLGEGLPPGGGL
                     GRTSLEGHIASKDKVTTLSGMRLMLDRANITGTLGIDSRSDVPSLTGVLSVDHLDLNP
                     YLAEGTKRDGGQPAAKAETGWSRTPFNLALLKKANARLTLDTGALRVRHLRLDKTVLG
                     VTLADGVLTARLDPVTLYGGSGRAELAIDGRGAVPAFRNRLKFEHVALQPFLNDTLGV
                     GRFEGAGALTLDIAAHGTNAHAVMRSLSGKGTLAGEQGRIRGVDLGAMARTIQNMLGG
                     TAGTGTATDFLTMGGSFAVASGVLTSKDFRLAGPLLSMTGTGDIDIGNRAIDFRLVPK
                     ASAKGLSIGIPFRVRGSWDHVHYAPEMGGMMKGVMQNLENGKAPFKGLFGGGNKPRDQ
                     NGQPEKKKKNVGDALKNMFGIH"
     gene            1607..2716
                     /locus_tag="VGC27_03505"
     CDS             1607..2716
                     /locus_tag="VGC27_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0281674.1"
                     /translation="MKRWIVVVLLAVLLLSVVVLFGYLWITHRGPKLTVASWAGQYGH
                     AQASALLVPFARRSGADVRLAEYDGGTADLAHQVTTGQYAWDVVDLELPDAVAACHQG
                     LFEPIDAAALPAGPNGAPAVQDFLAGAIGPCWVGSVVYSQVIAYDPHRFAGREPSSLA
                     DFFDLKNYPGPRALRRAGPKFNLEMALLADGVAPRDVYAVLSTPAGVARALAKLDTIR
                     SALVWWSGDAPPARMLAEGRAVFTTILNGDAFDAAMHGNPPGVIWDRQLYEFDVFGIP
                     KGTPKRDLAMDFIRFATRAENLAAVAGWVPYGPARMSALPYVGDNPDLKIAMTPHLPT
                     TPEHFATAFAIDNTWWRLHGADVMVLWQAWLAKEK"
BASE COUNT          516 a          954 c          904 g          478 t
ORIGIN      
        1 atcgagctgg aggtcaatgc cgagggccgc tcgaactgga ccttcgcaaa ggaggataaa
       61 agcaccggca gcggcggaag caagatcacg ctgcccgcaa acaccgaatt ctccggcatc
      121 aagatcaacg acggaagcat cttctatacc aacgacaaga ccggtacccg ccgtgcgatc
      181 gaacatgtca acgccgccat tgccatcacg cggctggacc agccggtcgc tatcgacggc
      241 aatctcaccc ggaacggcca tcgggtggat ttcgacgtca aggtcatcac gcccgagacc
      301 ctgctcggca accgcaccac gatgctcgac ctttcgctga cctccgacat gatgcaggcc
      361 ggtttcaagg gaggtctcag ccaggatggt gcgctggcgg gcacgctcaa attcgatacg
      421 gcatccttgc gcggcgtgct cggctggctc ggcgagggcc ttccgcccgg cggcgggctg
      481 ggccggactt cgctcgaagg ccatatcgca tccaaggaca aggtgacgac actgtcgggg
      541 atgcggctca tgcttgaccg cgccaacata accggcacgc tggggatcga ctcccgcagc
      601 gacgtgccgt cgctgaccgg cgtgctgtcc gtcgatcatc tcgacctcaa cccctatctg
      661 gcggaaggca ccaagcggga cggcggacaa ccggcagcca aggccgagac cggctggagc
      721 aggacgccgt tcaatctggc gctgctgaaa aaggcgaacg cccggctgac gctcgacacc
      781 ggtgcgctgc gcgtacgcca tctgcggctg gacaagaccg tcctcggcgt gacgctggcg
      841 gacggcgtcc tgactgcacg gctcgatccg gtcacgcttt acggcggcag cggccgggcg
      901 gaactcgcca tcgacgggcg cggcgccgtt cctgccttcc gcaaccggct caaattcgag
      961 catgtggcgt tgcagccgtt cctcaacgac acgctgggcg tcggccgctt cgaaggcgcg
     1021 ggcgcgctca ccctcgatat cgccgcgcat ggcacgaacg cccatgccgt tatgcgtagc
     1081 ctgtcgggca aaggcaccct cgccggcgag caaggccgca tccgcggcgt cgatctgggc
     1141 gcgatggcgc gcacgatcca gaacatgctg ggcggcacgg cgggcacggg cacggccacc
     1201 gatttcctca ccatgggcgg gtcgttcgcc gtcgccagcg gcgtcctgac cagcaaggat
     1261 ttccgcctcg ccggtccgct actgtcgatg acgggaacgg gcgacatcga catcggcaat
     1321 cgcgcgatcg attttcgcct cgtaccgaag gccagcgcca aaggcttgag catcggcatt
     1381 cccttccgcg tcagaggctc gtgggatcac gtccattacg caccggaaat gggcggcatg
     1441 atgaaaggcg tgatgcagaa cctcgagaac ggcaaagcgc cgttcaaggg cctgttcggc
     1501 ggcggcaaca aaccgcggga ccaaaacggc cagccggaaa agaagaaaaa gaacgtcggc
     1561 gacgccttga agaacatgtt cggtattcat tgaaaggatc ggatcgatga aacgctggat
     1621 cgtcgtggtc cttctcgccg tacttctgct ctcggtcgtg gtgctgttcg gctatctgtg
     1681 gataacgcat cgcggcccca agctgacggt cgccagttgg gcgggacaat acggccatgc
     1741 gcaggcgagc gcgcttttgg ttcctttcgc ccggcgcagc ggcgccgacg tgcgccttgc
     1801 cgaatatgac ggcggcacag ccgatctggc tcaccaggtc acaaccggcc aatatgcgtg
     1861 ggatgtggtc gatcttgaac ttcccgacgc cgtcgccgcc tgccaccaag gcctgtttga
     1921 gccgatcgat gcggcggcgc ttccggccgg ccccaacggc gcgcccgccg tgcaggattt
     1981 cctggccggc gccatcgggc cctgttgggt cggcagcgtc gtttatagtc aggtcatcgc
     2041 ctatgatccg caccggttcg ccggccgtga accttcgtcc ctcgccgatt tcttcgacct
     2101 gaagaactat cccggaccgc gcgcgctgcg ccgcgccggc cccaaattca acctggaaat
     2161 ggcgctgctg gcggacggcg tagcgccccg cgatgtttat gcggtgcttt cgacgccggc
     2221 gggcgtggcg cgcgcgctcg ccaagctgga cacgatcaga agcgcgctgg tgtggtggag
     2281 cggcgacgct ccgcccgcgc ggatgctggc ggagggccgc gccgtcttca cgaccatcct
     2341 caacggcgac gcgttcgacg cggcgatgca cggtaacccg cccggcgtga tctgggaccg
     2401 tcagctttac gaattcgacg tattcgggat tcccaaaggc acccccaaac gcgacctggc
     2461 gatggacttc atccgcttcg cgacccgagc cgagaatctc gccgccgtcg ccggctgggt
     2521 gccttacggg ccggcgcgca tgtccgcctt gccctatgtc ggcgacaatc cggacctcaa
     2581 gatcgccatg acgccacacc tgccgacgac gcccgaacat ttcgccaccg ccttcgccat
     2641 cgacaacaca tggtggcggc tgcacggcgc cgacgtcatg gtgctatggc aagcgtggct
     2701 ggcgaaggag aaatgaacgg tcccgtcgcg gcgcgcgccg accccgctac ttgatgcgct
     2761 tcactctgta ggtatgaacc aagtcgttga tctgcagact gtagccgcct agccggccgc
     2821 gcgagatcgt gacccggttc ttcctgccct tg
//
LOCUS       DASWPU010000155         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_13426,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000155 DASWPU010000000
VERSION     DASWPU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13426"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="VGC27_03510"
     CDS             <1..1816
                     /locus_tag="VGC27_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY0281675.1"
                     /translation="LSGFNILLAGGVNLAREPRNGRNFEYVGEDPLLAGEIVGASIRG
                     IQSMHIVSTLKHFAINNQETHRKTVNVTISTEAMRQSELLAFELAIEKGNPASVMCSY
                     NLVNGQWACENEYLLNRTLKRDWKYKGYVMSDWGGVHSTVDSANNGLDQFTGYPCCGM
                     VGAYFAPKYFKPALESGQIPMARLDDMVTRILTGVYASGAYDDPPVVGEIDFVKNFQV
                     SQNAAEQSFVLLKNEGNLLPLKNARQIAVFGGYADKGVLSGGGSANVMAVGGNAVPGL
                     EPVNWPGPVVYIPSSPVAALKKELPRADISYDDGTDITRAAEAAKKADVAIVFVTKFN
                     SEAIDGTLELDANQDQLVAAVAKANPNTIVVSEAGGAFLMPWIGDVKAVLNAFYPGSS
                     GGKAIARVLTGKVNPSGHLPISFPASNDQLAHPTIAGSNLPDSMSQDPKYATDITYDE
                     GAAIGYKWYDVKGYKPLFAFGHGLSYTSFATSNLKAAFEGNSLTVSFTVKNTGKLAGK
                     AVPQIYVEPADYTAAGWEAPKRLAGFSKLALKPGEMRTIKVKVDPRLLATYMTAANSW
                     QIKEGTYRIMLGQASDSLAQTVTVRLPAIIWSAVHSDQ"
     gene            2032..3042
                     /locus_tag="VGC27_03515"
     CDS             2032..3042
                     /locus_tag="VGC27_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0281676.1"
                     /translation="MATIKDVARRAGVGIGTVSRVITGRGIVSAKTAKKVHRIIDELA
                     YRPSRTARRLQAGMSHTIGVFLPVIHGAFYTPMLHAIYKALWEHELHMVVAFGKSPDD
                     ERQQAIEGVRFLTDHGCDGLLIMATALKLADVVSLTAVAPRIALLNRYFPSYAEACFI
                     PDHKKAGAVAAQALWQAGHRRVAVIEGPDYSKDNAARMRGFYKTMAACGVDIDGIPVF
                     RGDFAPSGGWAGAQSLLSGKKKFSALFCANDEMALGAISYLHHIGVSVPGDISVLGYD
                     GLDVATYAAPPLTTVRMPWTELALSAVNFLLNQCYEFTLPVERNQQATLVWRDSVAKI
                     GR"
BASE COUNT          681 a          947 c          917 g          612 t
ORIGIN      
        1 tttgagcggt ttcaacatct tgctcgccgg cggcgtcaat ctggctcgcg agccgcgcaa
       61 cgggcgcaac ttcgaatacg tcggcgagga tccgcttctg gccggcgaaa tcgtcggcgc
      121 ttcgatccgc ggcatccagt cgatgcacat cgtctcgacc ctgaagcatt tcgcgatcaa
      181 caaccaggag acccatcgca agacggtcaa tgtcacgatc tcgacggaag cgatgcggca
      241 gtccgagctt ctggccttcg agcttgcgat cgaaaagggc aatcccgcct cggtgatgtg
      301 ctcctataac ctcgtcaacg gccaatgggc ctgcgagaac gaatatcttc tgaaccgcac
      361 tctgaagcgg gattggaagt acaagggcta tgtcatgtcc gattggggcg gggtgcattc
      421 gaccgtggac tccgccaata acggcctcga tcagttcacc ggctatccgt gttgcggcat
      481 ggtcggggcg tatttcgctc ccaaatactt caaaccggcg cttgagagcg gccagattcc
      541 gatggcccgc cttgacgaca tggttacgcg catcctgaca ggggtctatg catcgggcgc
      601 gtacgacgat ccgccggttg tcggcgagat cgattttgtc aagaacttcc aagtttccca
      661 gaacgccgcc gagcaatcct tcgtgctgtt gaagaatgaa ggcaatctct tgccgctgaa
      721 gaacgcgcgc cagattgctg tgttcggtgg ctacgcggac aaaggcgttc tctcaggcgg
      781 cggttcagcc aatgtcatgg cggtcggtgg aaacgccgta cccggattgg aaccggtcaa
      841 ctggcccggg ccggtcgtct atatcccgtc ctcgccggtg gcggcactga agaaggagct
      901 gccgcgcgcg gacatcagct atgacgacgg aaccgatatc acccgcgcgg cggaagcggc
      961 caagaaggcg gacgtcgcta tcgtcttcgt caccaagttc aacagcgaag ccatcgacgg
     1021 cacgctggaa ctagacgcaa accaggacca actggtcgcc gcggtcgcca aagccaatcc
     1081 caatacgatc gtggtctcgg aggccggcgg ggcgtttctg atgccgtgga tcggcgacgt
     1141 gaaggccgtg ctcaacgcgt tctatcccgg cagttccggc ggaaaggcca ttgcgcgtgt
     1201 cctgaccggc aaggtcaatc cgtccggcca tcttcccatc agtttcccgg ccagcaacga
     1261 ccagctcgct catcccacca ttgccggcag caatctgccc gacagcatgt cgcaagatcc
     1321 gaaatacgcc accgacatca cctacgatga gggcgcggcc atcggctata aatggtacga
     1381 tgtcaaaggc tacaagccgc tcttcgcctt cggtcacggc ctcagctaca ccagttttgc
     1441 cacttcgaac ctcaaggcag cgttcgaagg aaattcgctg acggtcagct ttacggtgaa
     1501 gaacaccggc aagctcgcgg gcaaagccgt gccccagata tatgtggaac cagccgatta
     1561 cacagcggcc ggctgggaag cgcccaagcg tcttgccggc ttttccaagc tggcactcaa
     1621 gccgggtgag atgcgaacca taaaagtgaa agtcgatccg cgactgctcg caacctacat
     1681 gacggcggcc aacagctggc agatcaaaga agggacctac cgcatcatgc tcggacaggc
     1741 ctcggattct cttgcccaga ccgtaaccgt gcgcttgccg gcgataatct ggagcgcggt
     1801 gcattccgac cagtgaccaa gagccggtag taaagtgtgc ctcgtcaaca attacctgac
     1861 agaggcaagg agaaagcctc gccacttgtt gcaaaaagcg agcgacattg cgtccacaat
     1921 aaaatatcgc ccggaagcat attttcaaac ataattgtgc tcgcttgaag caggttgtct
     1981 ttaaaatgtc tgttcacgcg tttttcgtgc caagcaaagg gtcgatgcca gatggccaca
     2041 attaaggatg tcgcaagacg ggcgggcgtc ggcatcggaa cagtgtcgcg cgtgataacc
     2101 ggcaggggca tcgtgtcggc aaaaaccgcc aaaaaggtgc acaggatcat cgatgaattg
     2161 gcctatcgtc cgtcgcgaac ggcccgcagg ctgcaagccg gcatgtccca caccatcggc
     2221 gtatttctgc ccgtcatcca tggagcattc tacacaccca tgttgcacgc catctataag
     2281 gcgttgtggg aacacgagct gcacatggtt gtggcattcg gtaaatcgcc ggacgatgag
     2341 cggcagcagg cgatcgaagg cgtacgcttt ctcaccgacc atggctgcga tggcctgctc
     2401 atcatggcga cggcgctgaa actggccgat gtcgtcagcc tgaccgccgt ggcgccgcgg
     2461 atagctcttt tgaaccggta ttttcccagc tatgccgagg cctgcttcat ccccgaccat
     2521 aaaaaggccg gcgccgttgc agcgcaagcg ctatggcaag ccgggcatcg gcgggtggcg
     2581 gtcattgaag ggccggacta ctccaaggac aatgccgcac gcatgcgcgg cttttacaaa
     2641 accatggcgg cttgcggcgt cgatatcgac ggcattcccg tcttccgcgg cgacttcgcg
     2701 cccagtggtg gctgggccgg tgcacaaagc ctgctctcgg gcaagaagaa gttcagcgcc
     2761 ctgttctgcg ccaacgatga aatggccttg ggagccatct cgtatctgca ccatataggt
     2821 gtctcggtgc ccggcgacat atcagtgcta ggttatgacg ggctggatgt cgccacctat
     2881 gcggccccgc ccttgacgac cgtccggatg ccgtggaccg agcttgccct gagcgccgtc
     2941 aattttcttc tcaaccagtg ctatgaattc acgctgccgg tcgagcgcaa tcagcaagcc
     3001 accttggtgt ggcgtgattc cgtggccaaa atcggccgct gatgatcgca tcacgtcggc
     3061 gccatacgca ttcctgcgcc cacaccccgg ctagagcaga gtgcattcag acagaactac
     3121 ttgtcattcc cggccgagcg tagcgagggg aagggaa
//
LOCUS       DASWPU010000156         9947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_111479,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000156 DASWPU010000000
VERSION     DASWPU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111479"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VGC27_03520"
     CDS             complement(<1..448)
                     /locus_tag="VGC27_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEY0281677.1"
                     /translation="MDWLYLLHQAVFGAIAAAGFGVLFNFGLRSLVWCGVAGALALAV
                     RSLGQDAGWSLEAASFAAAVSTSCAMQLLREPLGAASNAMALAGCIPMVPGAFFSQAL
                     LGLFAVTAPDTANADGTILMAMEYMLRVIFTLAAIGAGLAIPAHLLR"
     gene            complement(448..1239)
                     /locus_tag="VGC27_03525"
     CDS             complement(448..1239)
                     /locus_tag="VGC27_03525"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEY0281678.1"
                     /translation="MTEKPDPVRLKHRRLEQIAMTALRASRLLTECGARVRVAHDGAA
                     LIVRGLGVDLVGLRTGYASQEVTVSSGANTITRMMTIGPHGVNHRLDHALRALAVRVS
                     KGGMSVEDTEREMDRLVKETPRHPAWFVAVAVGIACAAFGRLLGIDWVAFAPVLAAGA
                     VGQYMRHLLLKRGVNSYIVATAIAFLAAAVAGEGARLAGSTTVNLAMMASMLLLVPGV
                     PATNAQTDIMDGFPTMGSARAVSVIMVMVFATTGVWLAEALLGVE"
     gene            complement(1245..1634)
                     /locus_tag="VGC27_03530"
     CDS             complement(1245..1634)
                     /locus_tag="VGC27_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0281679.1"
                     /translation="MKKKADIRPIRVEADYDWALKEVEPYFVREPKRGTPEADRFDVL
                     AALIEAYEAKHWPIDPPDPVEAIRFRMDLSGWRQADLARLLGSRSRASEIMRRKRPLT
                     MEQAWKLHRKWNIPAEVLLRPYHTARI"
     gene            complement(1634..1930)
                     /locus_tag="VGC27_03535"
     CDS             complement(1634..1930)
                     /locus_tag="VGC27_03535"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEY0281680.1"
                     /translation="MQIIAKRTLKLFWARHPQAEIPLRNWHAVVDKAVWRGPADVKAM
                     FGTTVDFVEDNRLVFDIAGNKYRLVVHVAYRFKRVLVKFIGTHKDYNRIEAGSV"
     gene            complement(1969..3342)
                     /gene="gor"
                     /locus_tag="VGC27_03540"
     CDS             complement(1969..3342)
                     /gene="gor"
                     /locus_tag="VGC27_03540"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEY0281681.1"
                     /translation="MSHYDFDLFVVGGGSGGVRAARLAANLGRRVGLAEESRMGGTCV
                     IRGCIPKKLLVYAGHFAEDFELARSFGWSVGETSFDWFSLIAGKNREISRLEGLYRAA
                     VEGAGGTVFHERARFEDAHTLRLAGGREVTAGKILIATGAHPSRDLGGPAGAELCLTS
                     DEAFDLEELPGSIVIAGGGYVAIEFAHIFHALGVDVTLVYRGPKVLRGFDEDLRDALT
                     ASMARRGLRVITGSIFTSIEKLGGGLCAQTSTGQTLIADKIMLAIGRHPNTAGLGLDR
                     IGVATGSRGKIVVDEFSRTNIENIYAIGDVTDRLALTPVAIHDAVCFVKTAFEGTPTR
                     PDHTLVPTAVFSTPEIGTVGLSEARALELGHAIDVYKSSFRPLRLALAEGGDRTMMKL
                     VVGQETGKVLGCHIFGPEAAELIQLVAVALKMGATKAQFDATLALHPSAAEELVTMRT
                     KSYSKTP"
     gene            3412..4626
                     /locus_tag="VGC27_03545"
     CDS             3412..4626
                     /locus_tag="VGC27_03545"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0281682.1"
                     /translation="MARLRQKIGNFAVLALASLLAACAIDDSGPVRSVWRPADGKPST
                     INVVYLTDRQPDATAAAGFGQRWANEASCGVAETVIPPAMLPGEKPQYGYVAKTEPHA
                     CAKDKASLGGAVAAIEAQAKARNCNSVFLFVHGFHTGFDGAVLRAAQVSHDAQTGCAV
                     ASFSWSSEVKLNRYANDLEHSQYSEPLLAEFLRELAESGLQVKILAHSMGNRLVLATL
                     SGFARGREPVKAGFIGELVLAAADIGIEKDNDDFSHLLRDAAPYVKRTTIYASAGDAV
                     LVISRDAHGGVPRLGREPEASLAYRADDKTHIVDVIDASGAPADILDHSYFAMSYEAV
                     ADMTLALAGVPAADRLTAQGGWKPTLVCGNAKDLPCGKDSRHALAVGKGRHPRLVSRI
                     LVRLVPLIPFIQ"
     gene            complement(4616..5311)
                     /gene="rpiA"
                     /locus_tag="VGC27_03550"
     CDS             complement(4616..5311)
                     /gene="rpiA"
                     /locus_tag="VGC27_03550"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY0281683.1"
                     /translation="MTDSAALKRAAAARALDFVTDGMKLGLGSGSTAEAFVELLGARV
                     RGGLNLLCVPTSERTAQLARGLGIALANLDDLAPLDLAVDGADEVDPAFNLIKGGGGA
                     LLREKIVATSAKRMIIIVDGSKTVSRLGRFPLPVEVLEFGHATTAARIVRAVADLGYR
                     HTPIVLRKKDGAVYRTDNGNAIYDCAFGAIADAGKLSAALSCVTGVVEHGLYVGIAKM
                     LVVARPEGVEVIG"
     gene            5469..5966
                     /locus_tag="VGC27_03555"
     CDS             5469..5966
                     /locus_tag="VGC27_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEY0281684.1"
                     /translation="MAPKNGKPTAGAIDTGIPAAKRKAIAAGLSHLLADTYTLYLKTH
                     NYHWNVTGPQFNTLHLMFETQYNELALAVDLIAERIRALGYPAPGSYTQFAKLSVIKE
                     SQSVPTAEEMIADLVKGQEAVVRTARKVFPTADDAHDEPSTDLLTQRMQIHEKTAWML
                     RSMLE"
     gene            complement(6081..6245)
                     /locus_tag="VGC27_03560"
     CDS             complement(6081..6245)
                     /locus_tag="VGC27_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281685.1"
                     /translation="MFDERDVSTSAYHTCNRQAMDRAVAKVNSPLLAQRYGVPSLNAN
                     DALGRGANND"
     gene            complement(6456..8285)
                     /gene="glmS"
                     /locus_tag="VGC27_03565"
     CDS             complement(6456..8285)
                     /gene="glmS"
                     /locus_tag="VGC27_03565"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY0281686.1"
                     /translation="MCGIVGIAGTKDCAPVILEALKRLEYRGYDSAGIATLVNGRIER
                     RRAPGKLAKLGEALVKHPLPGHTGIGHTRWATHGKPSEANAHPHATERVAVVHNGIIE
                     NFRELRAELLAKGHKFESETDTEVCAHLVTDYLQQGMAPEAAVKAAVKRLTGAYALAM
                     IFKGEERLLIGARMGSPLAVGYGEREAYLGSDAFALAPFTNRVAYLDDGDVAVVRGFE
                     VAIFDASGRPANREVKITSATVGLVDKAGYRHFMAKEIHEQPDVVGHTLAHYLDTAGP
                     NVQLADGKVAAALARAARLTISACGTAYYAGLVGKYWFEKLARLPVEIDVASELRYRD
                     PVYPKDGLALFISQSGETADTLAALRDAKACGQTTFAIVNVAESSIAREADIVLPTFA
                     GPEIGVASTKAFTCQLSALAALAIAAGRARGVLDAADEKKLCTALLETPRQIVEFLKQ
                     EPKIEALGQEIAKAHDVLYLGRGPSFPLALEGALKLKEISYIHAEGYAAGEMKHGPIA
                     LIDEHVPVIVMAPHDALFEKTASNMQEVMARGGKVLLVSDAKGIASAGAQVWATVELP
                     DADPFIAPLLYAVPVQLLAYYAAVAKGTDVDQPRNLAKSVTVE"
     gene            complement(8414..9814)
                     /gene="glmU"
                     /locus_tag="VGC27_03570"
     CDS             complement(8414..9814)
                     /gene="glmU"
                     /locus_tag="VGC27_03570"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:NF010933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY0281687.1"
                     /translation="MAILRRNSSLYPYGTTDYRTSMTRAGIILAAGQGTRMKSATPKV
                     MHKVAGLPILGHVIAAMRAAGIERIVVVTHKEGEEVRAFAGKMGAASVVQEPQLGTGH
                     AAGVAAAALDGFSGALIVAYGDMPLLTAATFEASFAAQAKSGMAMVGFRPKDPGAYGR
                     MILDGNGLLDRIVEFKDAGAAERAEKLCNAGILAADAKDFFRWAAMLGNDNAQKEYYL
                     TDVPSFAKAEGVRCAVVEADETEMMGVNSRTELAAAEAAMQAWLRARALAGAAGMTAP
                     ETVFLSYDTVLEADCQIEPYVFFAPGVVVRSGARIRAFSHLEGAEVGAGAIVGPYARL
                     RPGAVLEENVHVGNFVEVKNTRLEKGAKANHLTYLGDARVGAGANIGAGTITCNYDGF
                     HKHKTDIGAGAFIGSDTALVAPVKVGDGAVTGAGSVITKDVAAGALVVTRAEQREIPG
                     WAEKFRTKKKAAKEKK"
BASE COUNT         1804 a         3368 c         3084 g         1691 t
ORIGIN      
        1 tccgcagcag atgggccgga atggccagac ccgcgccgat ggccgccagg gtgaaaatca
       61 cccgcagcat gtattccatg gccatcagga tcgtgccgtc ggcgtttgcc gtgtccggcg
      121 ccgtcaccgc aaacagccca agcagggcct ggctgaaaaa ggcgcccggc accatcggaa
      181 tgcatccggc caaggccatc gcattggagg ccgcgcccag cggctcgcgc agcaactgca
      241 tcgcgcagct ggtcgagacg gcggcggcaa acgaggccgc ttcgaggctc cagcccgcat
      301 cctggcccaa gctccgcacc gccagggcaa gcgcccccgc cactccgcac cagaccaggg
      361 agcgcagccc gaaattgaac aggacgccga acccggccgc ggcgatggca ccgaagaccg
      421 cctgatgcaa caggtacagc caatccatca ctccaccccc agcaaggcct cggcgagcca
      481 tacgccggtg gtggcgaaca ccatcaccat gatgaccgac accgcccgtg cgctgcccat
      541 ggtggggaag ccgtccatga tgtcggtctg ggcgttggtc gccggtacgc ccggcaccaa
      601 gagcagcatc gaggccatca tggcgaggtt gaccgtggtg ctgcccgcca agcgcgcgcc
      661 ctctccggcc acagccgccg ccagaaaggc gatggccgtc gccacgatgt aggaattgac
      721 gccgcgtttg agcagcaaat ggcgcatata ttggccgacc gcgcccgccg ccagcaccgg
      781 cgcgaacgcc acccaatcga ttccgagcaa ccgtccgaac gcggcgcagg cgatgcccac
      841 ggccacggcg acgaaccaag cggggtgacg gggcgtctct ttcaccaaac ggtccatctc
      901 gcgttcggtg tcctcgacgc tcatgccgcc cttggaaacg cgaaccgcca aggcgcgcaa
      961 ggcatgatcg aggcgatggt tcacgccgtg cgggccgatc gtcatcatcc gcgtgatggt
     1021 gttggcgccg ctcgacacgg tcacttcttg cgaggcatag ccggtgcgca atcccaccag
     1081 atccacgccg agaccgcgca cgatcaaggc ggcgccgtca tgcgccacgc gaacccgcgc
     1141 gccgcattcg gtgagcagcc gcgaggcgcg caacgccgtc atggcgatct gctccagccg
     1201 gcggtgcttc aggcgtacgg gatcgggttt ctcggtcatg gcggctatat acgggccgtg
     1261 tgataggggc gaagcagcac ttccgccggg atgttccatt tgcggtgcag cttccaggcc
     1321 tgttccatcg tcaaaggccg tttgcggcgc atgatctccg aggcccgcga acgcgacccc
     1381 agaagccgcg ccagatcggc ctgacgccag cccgaaagat ccatccggaa ccggatcgcc
     1441 tcgaccggat cgggcggatc gatcggccaa tgctttgctt cataggcctc gatcaaagcc
     1501 gcgagaacgt cgaaacggtc cgcttcgggc gtgccgcgtt tcggctcgcg cacgaaatac
     1561 ggctcgactt ccttgagcgc ccaatcgtag tccgcctcga ccctgatcgg acggatatcc
     1621 gctttcttct tcatcacacg cttcccgctt ctatcctgtt atagtccttg tgggtgccga
     1681 taaatttgac cagcacgcgc ttgaaccgat acgccacgtg gacgacaagg cgatatttgt
     1741 tgcccgcgat atcgaagaca agccggttgt cttcgacaaa atccaccgtc gtcccgaaca
     1801 tcgccttcac gtcggccgga ccccgccata cggccttgtc cacgaccgca tgccaattgc
     1861 gcagcgggat ttcggcttgc ggatgacgcg cccagaacag cttcaacgtc cgcttggcga
     1921 tgatctgcat gccggccgta tagcatgttc caattatggg aacaagcgct acggcgtctt
     1981 ggaatagctc tttgtccgca tggtcaccag ttcttcggcg gcgctggggt ggagcgccag
     2041 ggtggcgtcg aactgcgcct tggtggcgcc catcttcagc gccacggcga cgagctggat
     2101 cagctccgcc gcctcggggc cgaaaatgtg gcagcccagg actttgccgg tttcctgccc
     2161 gacgaccaat ttcatcatcg tgcggtcgcc gccttctgcc agcgccagcc gcaagggacg
     2221 gaagctcgat ttgtacacgt cgatggcgtg gcccagctcc agcgcccgcg cttcgctgag
     2281 accgaccgtg ccgatctccg gcgtcgaaaa cacggcggtc ggcaccagcg tgtgatcggg
     2341 ccgcgtcggc gtgccctcga acgccgtctt cacgaagcaa acggcatcgt ggatggcgac
     2401 cggcgtcagc gccaagcggt cggtcacgtc gccgatggcg tagatgtttt cgatattggt
     2461 gcgggagaat tcatcgacca cgatcttccc gcgcgagccg gtggcgacgc cgatgcggtc
     2521 gaggccgagc ccggcggtgt tcggatggcg cccgatggcg agcatgatct tgtccgcgat
     2581 cagcgtttgg cccgtgctgg tttgcgcgca gagcccgccg ccgagcttct cgatcgaagt
     2641 gaagatgctg ccggtgatga cgcgcaggcc gcggcgcgcc atcgaagccg tcagcgcatc
     2701 gcgcaaatcc tcgtcgaagc cgcgcagcac tttcggcccg cggtagacca gcgtgacgtc
     2761 cacgcccagg gcatgaaaaa tatgcgcgaa ttcgatcgcg acatagccgc cgcccgcgat
     2821 cacgatgctg cccggcagtt cctccaggtc gaacgcctcg tccgaggtga ggcaaagctc
     2881 cgcccccgcc ggcccgccca aatcgcgcga aggatgagca ccggtggcga tcagtatctt
     2941 gccggcggtg acttcgcgcc cgcccgcaag gcgcagcgta tgggcgtctt cgaagcgcgc
     3001 gcgctcgtgg aacacggtgc cgcccgcccc ctccacagcc gcccggtaca agccttcgag
     3061 gcgggagatt tcccgattct tgcccgcaat cagtgagaac cagtcgaacg acgtttcacc
     3121 gacgctccag ccgaagctcc gcgccagttc gaaatcctcg gcaaaatgac ccgcatagac
     3181 caggagcttc ttgggaatgc agccgcggat gacgcaggtt ccccccatcc gcgattcctc
     3241 ggccaagccg acgcgccggc ccaggttggc cgccagccgc gcggcgcgta ccccgcccga
     3301 accgccgccg acgacgaaaa gatcgaagtc ataatgagac ataaccgcat cctgcccttg
     3361 ccagacatcg cttaaccgag gtttgatggc gcatcaacgc cgaggggcgc tttggcaagg
     3421 ctcaggcaaa agatcggcaa tttcgccgtg ctggcgctgg caagcctgct ggcggcctgc
     3481 gcgatcgacg attccggccc ggtacgctcg gtctggcggc ccgcggacgg caaaccctcc
     3541 accatcaatg tcgtctatct gaccgatcgc cagcccgacg caaccgcagc ggccggcttc
     3601 ggccagcgtt gggccaacga agcctcctgc ggggtggccg agaccgtcat cccccccgcc
     3661 atgctgcccg gcgagaagcc gcaatacggc tatgtcgcca agaccgagcc gcatgcctgc
     3721 gccaaggaca aagcctcgct cggcggggcg gtggccgcca ttgaagccca ggccaaggcg
     3781 cgcaactgca actcggtctt cctgttcgtc cacggcttcc ataccggctt cgacggcgcg
     3841 gtcctgcgcg cggcccaggt ctcccacgac gcccagaccg gatgcgccgt cgcctcgttc
     3901 agctggagca gcgaagtcaa gctgaaccgt tacgccaacg acctcgagca cagccagtat
     3961 tccgagccgc tgctggcgga attcctgcgc gaactggcgg aatcgggatt acaggtcaag
     4021 atcctggcgc acagcatggg aaaccggctg gtgctggcga cgctttccgg gttcgcgcgc
     4081 gggcgcgaac cggtgaaggc gggcttcatc ggcgaactgg tgctggcggc ggcggatata
     4141 ggcatcgaaa aagacaatga cgatttcagc cacctgttgc gcgatgccgc gccctatgtg
     4201 aagcggacga cgatctacgc ctcggcgggc gacgccgtgc tggtgatctc cagagacgct
     4261 catggcgggg tgccgcggct ggggcgcgaa cccgaagcca gtctcgccta ccgcgccgac
     4321 gacaagaccc acattgtcga cgtgatcgac gcctccggcg cgccggccga tattctggac
     4381 cacagctatt tcgcgatgtc ctacgaagcc gtcgccgaca tgaccctggc gctggccggg
     4441 gtgccggcgg cggacaggct gacggcgcag ggcggctgga agcccaccct ggtctgcggc
     4501 aacgccaagg acttgccttg cggcaaggat tcgcgccatg ccctggccgt gggcaaaggc
     4561 cgccacccgc gcctcgtctc gcgcatcctg gtgaggttgg tgccgctgat tccgtttatc
     4621 caataacttc gacgccttcg gggcgcgcca ccaccagcat tttggcgatg ccgacatata
     4681 ggccgtgctc gacgacgccg gtgacgcagg acagcgccgc agagagcttg ccggcatcgg
     4741 cgatggcgcc gaaggcgcag tcgtagatcg cgttgccgtt gtcggtccgg taaacggcgc
     4801 cgtctttctt gcgcaggacg atgggcgtgt gccgatagcc caggtctgcc accgcacgca
     4861 cgatgcgcgc ggccgtggtg gcatggccga actccagcac ttccaccggc agcggaaacc
     4921 gtcccagccg ggacaccgtc ttgctgccgt cgacgatgat gatcatccgc ttggcggagg
     4981 tcgccacaat cttctcgcgc agcaaggcgc cgccgccgcc cttgatcaaa ttgaaagcgg
     5041 ggtccacttc gtcggcgccg tccaccgcca agtcgagcgg cgccagatcg tcgagattcg
     5101 ccagggcgat gccgagcccg cgcgccaact gcgcggtgcg ctcggaggtc ggcacgcaca
     5161 gcagattcag cccgccgcgc acgcgcgcgc ccaaaagttc gacgaacgcc tcggccgtcg
     5221 agccggagcc gagcccgagc ttcatcccgt cggtcacgaa gtcgagggca cgggcggcgg
     5281 cggcgcgctt gagggcagca gaatcggtca tggcggcgca atgaagccga tgatgcggcc
     5341 cgcgtccagc cttagccccc ctgaaagacg ggttcggcgc gggacatttt ggcccaagac
     5401 aatgtaaatg tcggcgacta ttgctatata tcgtatcgcg tacgaattcg aaccgaagga
     5461 gttcaacaat ggcaccgaaa aatggcaaac cgaccgccgg cgcgatcgac accggaatcc
     5521 ccgccgccaa gcgcaaggca atcgccgcag gcctgtcgca tctcctcgcg gacacctaca
     5581 cgctttacct caagacccat aattaccact ggaacgtcac cggcccgcag ttcaacaccc
     5641 tgcatctcat gttcgagacc cagtacaacg agctggcgct ggcggtcgat ctgatcgccg
     5701 aacgcatccg cgcgctgggt tatcccgcgc cgggcagcta tacgcagttc gccaagctgt
     5761 cggtgatcaa ggaaagccaa agcgtgccga cggccgagga aatgatcgcc gatctggtca
     5821 aaggccagga agcggtggtg cggaccgcgc gcaaagtgtt cccgacggcg gacgatgccc
     5881 acgacgaacc gagcaccgac ctgctcaccc agcgcatgca aatccacgaa aagaccgcat
     5941 ggatgctgcg cagcatgctg gaataggcca cccgccgtca cggacttcac gcgccagcgc
     6001 ggccgggaac gcataacggg cgctcccgcc aaggagcgct tgcgccatcc ggctaggccg
     6061 gtaaagcgct tcgagatggc ttagtcgtta tttgcaccgc gtccgagtgc gtcgttggca
     6121 ttcaggcttg gtacgccgta acgctgggca agcaagggtg aattgacctt tgccacggcg
     6181 cgatccatcg cctgcctgtt gcaggtatgg taggccgaag tgcttacatc gcgttcgtcg
     6241 aacacgcggc cgcaaaccgc cttcgcagca cgccaaagta gaaattatta ggatgccgcc
     6301 cctcgccgga ctgtcaggtg attacgatct ctgtacagct ctgttggaaa tctgaaataa
     6361 tgcaagtgtt gaaacgcatg gatccccttc cctcgccgcg caaagtgcgc ggctcgccgg
     6421 ggatgacaat gagaattaat tcaaaataac ttcgcctact ccaccgtcac ggacttcgcg
     6481 aggttgcgcg gctgatcgac gtcggtgcct ttggcgaccg cggcgtaata ggccaggagc
     6541 tgcaccggta cggcgtaaag cagcggcgcg atgaagggat cggcgtcggg aagctccact
     6601 gtcgcccaca cctgggcgcc ggcgctcgca atccccttcg catcggagac gagcagcacc
     6661 ttgccgccgc gcgccatcac ctcctgcatg ttggacgcgg tcttctcgaa cagcgcgtcg
     6721 tgcggcgcca tcacgatcac cggcacatgc tcgtcgatca gcgcgatggg gccgtgcttc
     6781 atctcgccgg cggcgtagcc ttcggcgtgg atgtaggaga tttccttgag cttgagggcg
     6841 ccttccagcg ccagcggaaa actcggcccg cggccgagat agagcacgtc gtgcgccttg
     6901 gcgatctcct ggcccagcgc ttcgattttc ggttcctgct tgaggaattc gacgatttgg
     6961 cgcggcgttt cgagcagcgc cgtacacagc ttcttctcgt cggcggcgtc gagcacgccg
     7021 cgcgcgcgac cagcggcgat ggcaagcgcc gccagcgccg aaagctgaca ggtgaacgcc
     7081 ttggtcgagg cgacgccgat ctccggaccg gcgaaggtcg gcagcacgat atcggcttcg
     7141 cgcgcgatgg agctttcggc cacgttgacg atggcgaagg tggtctggcc gcacgccttg
     7201 gcgtcgcgca acgccgccag cgtatcggcg gtctcgccgg actgcgaaat gaacagcgcc
     7261 agcccgtcct tgggatagac cgggtcgcgg tagcgcaact ccgaggccac gtcgatttcc
     7321 accggcagcc gcgccagctt ttcgaaccag tattttccga ccagcccggc gtaataggcc
     7381 gtgccgcagg ccgagatggt gagccgcgcg gcgcgcgcca aagccgccgc gactttccca
     7441 tcggcgagct gcacattggg gccggcggta tcgagatagt gcgccaacgt atgaccgacg
     7501 acatccggct gctcgtggat ttccttggcc atgaagtgac ggtagccggc cttgtcgacc
     7561 aagccgacgg tggccgaggt gattttaact tcgcggttgg caggacggcc ggacgcatcg
     7621 aagatcgcaa cctcgaatcc gcgcaccacg gcgacgtcgc cgtcgtccag ataggccacg
     7681 cgattggtga acggcgccag cgcgaaggcg tccgatccca gataggcttc gcgctcgccg
     7741 taaccgacgg ccagcgggct tcccattcgc gcgccgatca agagcctctc ctcgcccttg
     7801 aaaatcatgg cgagcgcata ggcgccggtc agccgtttta ccgccgcctt cactgccgct
     7861 tcgggcgcca tgccttgttg gagataatcc gtgaccagat gggcgcagac ctcggtgtcg
     7921 gtctcggatt cgaatttatg ccccttggcc agcaattcgg cgcgcagctc gcggaaattc
     7981 tcgatgatcc cgttatggac gacggcgaca cgctcggtgg cgtgcggatg ggcgttggcc
     8041 tcggacggct tgccgtgggt ggcccagcgg gtatggccga tgccggtgtg accgggcagc
     8101 gggtgcttta ccagggcttc gcccagcttg gcgagcttgc ccggcgcgcg ccggcgctcg
     8161 atgcgaccgt tcaccagcgt ggcgatgccg gcggaatcgt agccgcgata ttccagccgc
     8221 ttcagcgcct ccagaatgac cggcgcgcaa tccttggttc ccgcgatgcc gacgatgccg
     8281 cacattcttt tccgtcctta ctccgcttca tccttcgaca gtcggtagcg acagcgtacc
     8341 gaccaggatg aaggctccat tcaaactccc ccaccccgag cttgccgaag ggtgagccgc
     8401 cgatgcacga agtctatttc ttttccttcg ccgccttctt ctttgtacgg aatttctccg
     8461 cccaacccgg aatttccctc tgctcggcgc gggtcacgac cagcgcaccg gccgccacgt
     8521 ccttggtgat gaccgaacca gcccccgtga cggcgccgtc gccgaccttg accggcgcca
     8581 ccagcgccgt gtcggaaccg atgaaggccc cggcgccgat gtcggtcttg tgtttgtgga
     8641 acccgtcgta attgcaggtg atggtgcccg cgccgatatt ggcccccgcg ccgacgcgcg
     8701 cgtcgccgag ataggtcaga tggttcgcct tggcgccctt ttcgagccgc gtgttcttga
     8761 cctcgacgaa attgccgaca tggacgttct cttccagcac cgcgcccgga cgcagccgcg
     8821 cataaggccc gacgatcgcg cccgcgccca cttcggcgcc ttcgagatgg ctgaacgcgc
     8881 gaatgcgcgc gcccgaacgc accacgacgc cgggcgcgaa gaaaacgtaa ggctcgatct
     8941 ggcaatccgc ttccagaacg gtgtcgtagg acaggaaaac cgtttccggc gcggtcatgc
     9001 cggcggcccc cgccagggcg cgggcgcgca gccacgcctg catcgccgct tccgccgccg
     9061 ccagctcggt gcggctgttg acgcccatca tctcggtctc gtccgcctcc acgacggcgc
     9121 agcgcacgcc ttcggccttg gcgaaggacg gcacgtcggt gaggtaatat tccttctggg
     9181 cgttgtcgtt ccccagcatt gcggcccagc ggaagaaatc cttggcatcg gcggcgagaa
     9241 tgcccgcatt gcataatttc tcggcgcgct ccgccgcacc ggcatccttg aattcgacga
     9301 tgcggtccaa aaggccgttt ccgtcgagga tcatgcgccc gtaggcaccc ggatccttgg
     9361 gacggaatcc gaccatcgcc atgccggatt ttgcctgcgc cgcgaacgat gcctcaaaag
     9421 tcgccgcggt cagcagcggc atgtcgccgt aagcgacgat cagcgcccct gaaaagccgt
     9481 caagcgccgc cgccgcaacg cccgccgcat ggccggtgcc aagctgaggc tcctggacaa
     9541 cgctcgcagc gcccattttt ccggcaaagg cgcggacctc ttcgccttcc ttgtgagtga
     9601 ccacgacaat gcgctcgatc ccggccgcgc gcatggcggc gatcacgtgc ccgagaatgg
     9661 gaaggcccgc gaccttgtgc atgaccttgg gcgtcgcgga tttcatccgt gtcccctgcc
     9721 ccgcggccaa tatgattccc gcacgcgtca tgcttgttcg atagtccgtc gtgccataag
     9781 ggtaaagtga agaattacga cgcaatattg ccatcgtttg agatcaatta acccactatg
     9841 acccctttca ccctggtttt cgacctcgac ggaacccttg tcgacaccgc gcccgacctg
     9901 ctcatcgccc tgaacgtcgt tctcgcgagt gagggacgcc gtgccgt
//
LOCUS       DASWPU010000157         4304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_74612,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000157 DASWPU010000000
VERSION     DASWPU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74612"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VGC27_03575"
     CDS             complement(<1..368)
                     /locus_tag="VGC27_03575"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEY0281688.1"
                     /translation="MKKNAGGGRGMGTAGMLPVLAMAGLVAAAPAFGQAEAGAQGTDV
                     WTQDTLTGDWGGGRSRLADSGITLGLAEISEVLGNVSGGQKQAGTYMGRTELVVDLDL
                     DKLVGWKGATIHVNGFQIHGR"
     gene            644..2581
                     /locus_tag="VGC27_03580"
     CDS             644..2581
                     /locus_tag="VGC27_03580"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEY0281689.1"
                     /translation="MKTILVALLFVAHLAGMAGAAESKPDYRGMVAKIDSMLAEAASD
                     YRAGKVDAAKTTVQHAYFQVFESMEGPIRINISAKHSYELESEFGAIRKLMSDGAPPA
                     EVERRIKAHITALNEVVPTLESGAVLVAEKTEGLDEPSPEAAQPALSQKIEPYWQTVV
                     ASIHDDLAAAADALDKGDAAKATALINHARFEGYKNSELTIAIRRYVSQQQDGAYEAE
                     FLRIAGLVKEGRPEQIRGSARVLAEELTDTLPGLPLVGAAAEAAAGSAPSADWVAVAD
                     KVNIAVSQAQALAADGKADAAVDLLQTTYFDVFEASGMEARIGARDPAFKTAIEAHFS
                     KLMALINKGAAPAALAAEAEAMKSDLVRAAALLGGEKSGTPWALFGYALLIILREGFE
                     AMVIVTAVIAYLVKTGHGDKQRVIGNSVFFAILASVATAALLKLAFREAAASQEVLEG
                     GTMLAAAATLFFVSFWLISKAEAKKWTAYIKGKVDSSLSSGSMKALWFTCFLAVYREG
                     AETVLFFQALTVGANTMGLFAVAGGFVAGCLGLGLLYWGMRAGALKLAIRPFFQGTGA
                     LLYLMAFVFTGQGIMELVEGKVFTPTLITGAPEIPVLGIYPYWQSLLPQAFLVAAALI
                     SLVVILRRRPGTPAAAETGAG"
     gene            2648..3187
                     /locus_tag="VGC27_03585"
     CDS             2648..3187
                     /locus_tag="VGC27_03585"
                     /inference="COORDINATES: protein motif:HMM:NF022101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HEY0281690.1"
                     /translation="MKTLISLSLALMAIGAIAAAGGAKGAENPESPIGETKAVGTLDI
                     GAVYLNPIEMEPHGIDLAASKADIHLEADIHARKGNPNGFGAGEWVPNLTISYRLENK
                     DTGKKISGNLMPMVASDGPHYGSNIKMLGTGNYHLTFHVDPPSKQGFGRHTDKATGVG
                     PWFQPFNVDYDFSYVQKKK"
     gene            3269..>4304
                     /locus_tag="VGC27_03590"
     CDS             3269..>4304
                     /locus_tag="VGC27_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281691.1"
                     /translation="MAYYIAALAHALVPAILIIGLWHAAAGADRRYRTGMGVSAGAVA
                     GLFAILTAALSTSETSIGVALHVAAFVAEIAALVLLLFCAFVTGRKALPKIGAALFRL
                     RSGFGPVLAAILIAQGLFGALSGIADHGLSATDVVNTDLIVNCFAILIGIALLAILAV
                     VLGRVARAAGGIYTTTALALVLTLALVTESGQLVLGLLRLELTDVTAGRISYVASLSL
                     ADPWLVYADLALCLALAVIAYGRRFRMAPLLSRVEQRLQLARTLSERRWRNGLAAIAA
                     LLLALLAYQDLYASLPPALSPAEPVEADDSGHVRIPVDQVKDSKLHRFAYITEAGHRV
                     RFFLLNRYDEA"
BASE COUNT          772 a         1378 c         1337 g          817 t
ORIGIN      
        1 cggccgtgga tttgaaagcc gttgacatgg atggtcgcgc ccttccagcc caccagcttg
       61 tcgagatcga gatcgacgac cagttcggtc cgccccatat aggtgccggc ctgcttctgg
      121 ccgcccgaga cattgcccag cacttcgctg atttcggcaa gacccagggt aatgccgcta
      181 tcagccagcc tggagcgccc cccgccccaa tctccggtca gcgtgtcctg cgtccacaca
      241 tcggtgccct gcgcgccggc ttccgcctgg ccgaaggctg gcgccgccgc aacaagaccg
      301 gccatcgcca gcacgggcaa catcccggca gtccccattc ccctgccccc gcccgcattc
      361 ttcttcatcg atgactccct cccaggttga aaattaggaa ttgcgacacc ctcaaaattt
      421 gccccccagg tgtcttaata ccctcacaga tgatgttgaa aatcattatc aaacgcagcc
      481 gtcaaggagg gtgcgtctaa ttgccgtaga aaagttgcgg gtattgacgt tgagaattat
      541 tcgcaatacc tacgggcgcc gacagcagcc ctgccgatgc aaggacggcg gatgacacgg
      601 cccaatccgg cgcgaagaca aagagagaca ggggaaaatg gggatgaaga cgattttggt
      661 ggcgttgctg ttcgtcgcgc atttggccgg tatggccggg gcggcagaga gcaagcccga
      721 ttatcggggg atggtcgcca aaatcgattc gatgctggcc gaagcggcgt cggattaccg
      781 cgccggcaag gtcgatgcgg caaagaccac cgtgcagcac gcctatttcc aggtcttcga
      841 aagcatggaa ggccccatcc ggatcaatat ctcggcgaaa cacagctacg aactggaaag
      901 cgaattcggg gccatccgca agctcatgag cgacggcgcc ccacccgccg aggtggaaag
      961 gcgcatcaag gcgcacatca cggctttgaa cgaggtggtg ccgacgcttg aaagcggcgc
     1021 cgtgctggtg gcggagaaga cggaagggct ggacgagccg tcgcccgaag cggcccagcc
     1081 agccttgtct cagaaaatcg aaccttattg gcagacggtg gtcgcctcca tccatgacga
     1141 tctggcggcc gcggccgatg ccttggacaa aggcgatgcc gccaaggcaa ccgcgctcat
     1201 caaccatgcc cgctttgaag gctataagaa ttccgaactc accatcgcga tccgccgcta
     1261 cgtctcccaa cagcaggacg gcgcttacga agcggagttc ctgcgcatcg cgggcttggt
     1321 caaagagggc cgtccggagc agatccgcgg ctcggcccgg gttctggccg aggaactaac
     1381 cgacaccctg cccggcctgc ctctggtcgg cgcggctgcg gaagcggccg cgggatccgc
     1441 cccctctgcc gactgggttg ccgtcgcgga caaagtgaac attgccgttt cccaggcgca
     1501 ggcccttgcc gccgacggga aggccgacgc ggccgtggac ctgctgcaaa ccacctattt
     1561 cgatgtcttc gaggcgagcg gcatggaggc ccgcatcgga gcccgcgatc ccgcttttaa
     1621 aaccgccatc gaggcccatt tcagcaaatt gatggcgctg atcaacaaag gcgccgcccc
     1681 ggccgcgctg gcggccgaag ccgaggccat gaaatccgat ctggtacgcg ccgcggctct
     1741 gctgggcggc gaaaaaagcg gcacgccctg ggccctgttc ggttacgcgc ttctgatcat
     1801 cctgcgcgaa ggcttcgaag cgatggtgat cgtgaccgcg gtcatcgcct atctcgtcaa
     1861 aaccggtcat ggcgacaagc agcgggtgat cggcaacagc gtgttcttcg ccatcctggc
     1921 cagtgtggcg acggcggccc tgctcaagct ggcattccgc gaggccgccg ccagtcagga
     1981 agtgctggaa ggcggcacca tgctggccgc cgccgccacg ctgttcttcg tgagcttctg
     2041 gctgatctcg aaggccgaag caaaaaaatg gacggcctac atcaagggca aggtcgacag
     2101 ttccttaagc tcgggttcga tgaaggcctt gtggttcacc tgtttcctgg ccgtctaccg
     2161 cgagggggcg gagaccgtcc ttttcttcca ggccttgacc gtcggcgcca ataccatggg
     2221 gctcttcgcc gtcgccgggg gattcgtcgc gggctgcctg gggcttggcc tgctctattg
     2281 gggcatgcgg gcgggcgcac tgaagctggc catccgcccc ttcttccagg gcacgggcgc
     2341 cttgctttac ctgatggcct tcgtcttcac cggccagggc atcatggagc tggtcgaggg
     2401 caaggtattc accccgaccc tgatcacagg cgcgcccgag atcccggtgc tcggtattta
     2461 tccttattgg caaagcctgt tgcctcaggc gttcctggtg gccgccgccc tcatttcatt
     2521 ggtcgtcatc cttcgccggc gtcccggcac accggccgcc gcggaaacgg gcgcgggatg
     2581 attttctttc ggaacacaca ttggccgtgg cggtgctgag cccgccctaa caagaaggag
     2641 cgcaagaatg aagacgttga tcagcttatc cctggccctg atggcaatcg gggcgatcgc
     2701 cgcggcgggg ggggcaaaag gtgccgaaaa tccggaatcg cccatcggcg aaaccaaagc
     2761 cgtgggtacg ctggacatcg gtgcggttta cctgaacccc atcgagatgg agccgcacgg
     2821 catcgatctg gccgcatcga aggccgatat ccacctggaa gccgatatcc acgcccgcaa
     2881 gggaaacccg aacggcttcg gcgcgggcga atgggtgccc aacctgacca tctcctatcg
     2941 cctggaaaac aaggatacgg gcaaaaagat cagcggcaat ctgatgccca tggtggccag
     3001 cgacggcccc cattacggct ccaacatcaa gatgctcggc accggaaact atcacctgac
     3061 cttccacgtc gatccgcctt cgaagcaggg attcggccgc cacaccgaca aggccaccgg
     3121 ggtcggcccg tggttccagc cgttcaacgt ggattacgat ttctcctatg tgcagaagaa
     3181 aaaataggtt tcgttcgggc gtgaatccgg gcccccgttc ctggcggaac gggggctttc
     3241 cccgttttcg gatacggagt taaagccgtt ggcctattac atcgccgcgc tggcgcacgc
     3301 cctggttccg gcgattctga tcatcggcct gtggcatgcg gcggcggggg ccgaccggcg
     3361 gtatcggacg ggcatgggcg tcagcgccgg tgccgtggca ggcctctttg ccatcctgac
     3421 cgccgcctta agtaccagcg agacgtccat cggcgtggcg ttgcacgtcg ccgccttcgt
     3481 ggcggagatc gcggccctgg tgctccttct cttttgcgcc ttcgtgaccg ggcgaaaggc
     3541 gttaccgaaa atcggcgcgg cgttgttccg gctgcggtcc gggttcggtc cggtgctggc
     3601 cgccatcctg atcgctcagg ggcttttcgg cgccttgagc ggcatcgccg atcacggcct
     3661 gagcgcgact gatgtcgtca ataccgacct gatcgtcaat tgctttgcaa ttctcatcgg
     3721 cattgccttg ctcgctatcc tggccgtggt gctcggccgg gtcgcacgcg cggccggcgg
     3781 gatttacacc accacggctc ttgccctcgt tctgaccctg gcgctggtga cggaatcggg
     3841 ccagctcgtc ctcggtctgt tgcgccttga actgaccgat gtcacggcgg ggcgcatttc
     3901 ttatgtcgcg tcgctgtcgc tggcggatcc gtggctggtc tatgccgatc tcgcgctctg
     3961 cctggccctg gccgtgatcg cgtacggccg ccggtttcgc atggcccccc tcctcagccg
     4021 cgtggagcag cgtcttcagc ttgcccgcac cctgtcggaa cggcgctggc gcaacggact
     4081 ggcagccatc gcggcgcttc ttttggcgct gttggcctat caggatttat atgcgtcctt
     4141 gccgcccgct ctgtccccgg ccgagccggt agaggccgat gacagcgggc atgtccgcat
     4201 cccggtggac caggtcaagg acagcaagct gcaccgtttc gcctacatca cggaggcggg
     4261 acaccgggtg cggtttttcc tgttgaatcg ttatgacgag gcgc
//
LOCUS       DASWPU010000158         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_68740,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000158 DASWPU010000000
VERSION     DASWPU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68740"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VGC27_03595"
     CDS             <1..884
                     /locus_tag="VGC27_03595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0281692.1"
                     /translation="RFAHALLDEVPGAKIVAVEPDERVAQSCAGLERCELHIARIEDT
                     ALESGRFDVIHSCHTIEHLAHPARVLADHWRCLKDGGLMLLDAPNIALIGADDIVEEW
                     FIDKHLTHFSARTLIRMVEAAGFSIVEAPDPADRENLFLVAKKSAPADHATEGDPEEV
                     ERAQALLAAYVAGRARNLAALGAVAAELTALAPKGVAMWGAGRIFDSLVVHGRFDARA
                     LRLLIDTHLKAHVGERHGCTLVGPEALAGADPGVIVVMSRGFAGEIAAEARKFAPAAE
                     ILLYTDLLSRARTRLAA"
     gene            896..1813
                     /locus_tag="VGC27_03600"
     CDS             896..1813
                     /locus_tag="VGC27_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0281693.1"
                     /translation="MNIAANLAVRNRSAAIPEIAHETDIETQIAEAALFVRRHTIASI
                     YRAESGHPGGALSCADIMACLFGAEMNVWPDGIGDPTRDRFVLSKGHACPALYAIGAY
                     HGFCDKTEALTLRKLGSPFQGHPHVHDLPWVETSTGSLGQGFSVALGMAMGLKLQKIQ
                     ARAYVLIGDGEMQEGEVWEAAMCAAHHKLDNLCVLIDYNKLQSDASNDVIMRLEPLAD
                     KWRAFGWTVFELDGHNIPEILSTLHLAASTHDRPSVLIAHTVKGKGVPYMEGSPAWHG
                     SVKLTRSQAEEALAAMGTEPQEIKELLDV"
BASE COUNT          351 a          665 c          598 g          332 t
ORIGIN      
        1 gccgcttcgc gcacgccctt ctggatgaag tacccggcgc aaaaatcgtc gcggtcgaac
       61 cggacgaacg ggtggcgcaa tcctgcgccg gcctggagcg ctgcgaactg cacatcgcac
      121 gcatcgagga caccgcgctc gaaagcggac gcttcgacgt catccattcc tgccacacca
      181 tcgagcatct ggcgcatccc gctcgggttc tggccgatca ttggcgctgc ttgaaggacg
      241 gcggcctcat gctgctcgac gccccgaaca tcgcgctgat cggcgccgac gacatcgtcg
      301 aggaatggtt catcgacaag catctgacgc atttttccgc gcgcacgctg attcgcatgg
      361 tggaagcggc gggcttttcg atcgtcgaag caccggaccc ggccgaccgg gaaaatctct
      421 tcctggtggc gaagaaatcc gcgcctgccg accatgcgac ggaaggcgat cccgaggaag
      481 tcgaaagagc gcaggctttg ctcgcggctt atgtcgccgg ccgggcacgc aatctggcgg
      541 cgctgggcgc cgtcgccgcc gaactgaccg cgcttgcgcc caaaggcgtg gcgatgtggg
      601 gcgcggggcg gattttcgac agtctggtgg tgcatggccg cttcgatgcc cgtgcactca
      661 gactgttgat cgatacccat ctcaaggccc atgtcggcga gcggcacggt tgcacgcttg
      721 tcggtcccga agctctggcg ggcgccgatc ccggcgtgat cgtcgtcatg tcgcggggct
      781 tcgcgggcga aatcgcagcc gaggcgcgca agttcgcgcc cgccgccgaa atccttctct
      841 acaccgatct actaagccgc gcgcgcacgc gcctggctgc ttgaggggag ttgccatgaa
      901 cattgccgca aacctagccg tccgaaaccg ctccgccgcg atcccggaga tcgcacacga
      961 aaccgacatc gaaacgcaaa tcgccgaggc cgccctgttc gtgcggcgtc acaccatcgc
     1021 gtcgatctac cgcgccgaat ccggccatcc aggaggggcg ctttcctgcg ccgatatcat
     1081 ggcctgcctg ttcggcgccg agatgaatgt ctggccggac gggatcggcg atccgacgcg
     1141 cgaccgtttc gtgctctcga agggccatgc ctgccccgcg ctctacgcca tcggcgccta
     1201 tcacgggttc tgcgacaaga ccgaagcgct cacgctgcgc aagctcggca gccccttcca
     1261 aggccaccca catgtgcacg atctgccctg ggtcgaaacc tcgaccggtt cgctcggcca
     1321 gggcttctcc gtcgccctcg gcatggcgat gggcctcaag ctgcagaaga tccaagcgcg
     1381 cgcttatgtc ctcatcggcg acggcgaaat gcaggaaggc gaggtctggg aggccgcgat
     1441 gtgcgccgcc caccacaagc tcgacaatct ctgcgtcctc atcgactaca acaagctgca
     1501 gagcgatgcg tccaacgacg tcatcatgcg gctcgaaccg ttggccgaca aatggcgcgc
     1561 cttcggctgg acggtgttcg agctcgacgg gcacaacatt cctgaaatcc tctcgaccct
     1621 gcatctggcg gcgtccaccc atgatcggcc ttcggtcctc atcgcccata cggtgaaggg
     1681 caaaggcgtg ccttacatgg aaggcagccc ggcctggcac ggcagcgtca aactcacgcg
     1741 ttcgcaggcc gaagaggccc tcgccgcgat gggtacggaa ccgcaagaaa tcaaggagct
     1801 tctcgatgtc tgacaccaat tccgctcccg ccctgatcgg ctcatcgggc gattcgctgc
     1861 gcgaagcctt cggcaaggcc ctggccggca tggccggcga atttcccaag ctcgtcgtac
     1921 tcgacgccga cgtcgccggc ggcacc
//
LOCUS       DASWPU010000159         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_99252,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000159 DASWPU010000000
VERSION     DASWPU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99252"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="VGC27_03605"
     CDS             <1..1677
                     /locus_tag="VGC27_03605"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEY0281694.1"
                     /translation="GPHTLSVPQGSILNLRVHGADHMPSVTLEGARFEGGDGEYAASA
                     PLAADGHVRVRASGHTIGSWKIAVIPDRKPTIAFTAPPSATDRQALKLSYKAGDDYGV
                     VAARAVIRPQGRKADPIIIDLQLPGRSATAVAQTMFRDLTEHPYAGLNVDITLEARDA
                     AGQTATSNTASFRLPQRNFTDPLARALIEQRQNLASLGNAAKVLRTLDALTLAPDKFY
                     AQAMGTYLALRAAVWGLKYAKNRQDIQRIEDLLWQIAVGLEQGGILTMAEQLRRMQQT
                     LSQMMAQGAPQDEIDAMFQRYQDLLQRYLQALAENAPQTGVPLDPNAKVLGAQDLAAL
                     LKAIEQLSQDGDQLRAMQLLAMLQEMIENLQVAGGAGGPGGGNAAADQALRDLSELMG
                     KQRMLLDKTFRQSQGTGDPKDGGAKGLAQQQGQLQNDLGALLKKGGKSPSQGDLQRAE
                     KMMGEAAQALGLGDLPRAGTLQKNILDALRKGAEAMAAGRGKGQQGAQDPLGRAAEGR
                     GAGTGGNLRIPDASVLQRARDILNELRKRAGEQKRPKEELDYIDRLLKQF"
     gene            complement(2043..2561)
                     /locus_tag="VGC27_03610"
     CDS             complement(2043..2561)
                     /locus_tag="VGC27_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY0281695.1"
                     /translation="MHSEILFSESVIAGRVTDMAQRIAAAPVKPEIAVPILVGAYVFA
                     GDLLRALAREGISLPTEFLWLRSYVGRTATKEISVLIGPNENFRGKNVLLIDGVLDGG
                     HTIHKARELAIAAGAASVRSAVVVDKARKDAVAVSDYAAFTAVPEFIVGYGMDDAGKD
                     RALPYIAKAGLI"
     gene            complement(2572..3084)
                     /locus_tag="VGC27_03615"
     CDS             complement(2572..3084)
                     /locus_tag="VGC27_03615"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEY0281696.1"
                     /translation="MQPPSYEAEPQERQQEAPKPRSLWPRRLLLVTGWAALAAVVVLI
                     GWTASVYRQQIVAKWPQSASLYSTLGMKTDSSGLRIVDYRYHEEIQGGQPMLVVTGKL
                     VNVSGSELSVPQIRVTLKDEARRELYHWSFMPAVISLRPGQSTPFVTRLSSPPADARH
                     LDLRFARAGE"
BASE COUNT          691 a         1121 c         1095 g          575 t
ORIGIN      
        1 gggccgcata cgctttccgt accgcaggga tcgatcctga atctcagggt ccacggcgcc
       61 gaccatatgc cgtcggtcac cctggagggc gcccgcttcg aaggcggcga cggtgaatat
      121 gcggcaagcg cgccgcttgc ggcggacggg catgtgcggg tgcgggcaag cggacatacg
      181 atcggaagct ggaagatcgc cgtcattccc gaccgcaaac ccaccatcgc ctttaccgcg
      241 ccgccgtccg cgaccgaccg tcaggcgctc aagctctcct ataaggccgg cgacgattac
      301 ggcgtcgtcg ccgcgcgcgc cgtcatccgc ccgcaggggc gcaaagccga tcccatcatt
      361 atcgatctgc aactgccggg acgctcggcg acggcggtgg cgcagaccat gttccgcgat
      421 ttgaccgaac atccttacgc cggcctcaac gtcgatatca ccctcgaagc ccgcgacgcc
      481 gccgggcaaa cggcgaccag caacaccgca agcttcagac tgccgcagcg caacttcacc
      541 gatcccttgg cccgcgcctt gatcgaacag cgccagaatc tcgccagcct gggcaacgcg
      601 gcaaaggtgc tgcgcacgct cgacgcgctg acgctggcgc cggacaaatt ctacgcccag
      661 gcgatgggca cctatctggc gctgcgcgcc gccgtctggg gactgaaata cgccaagaac
      721 cggcaggaca tccaacgcat cgaggatctg ttgtggcaga tcgccgtcgg cctggagcaa
      781 ggcggcatcc tcaccatggc cgaacaattg cgccggatgc agcagacttt gtcgcaaatg
      841 atggctcaag gcgcgccgca ggacgaaatc gatgcaatgt tccagcgcta tcaggattta
      901 ttgcagcgct accttcaggc gctggccgag aacgcaccgc aaacgggcgt cccgctcgat
      961 ccgaatgcga aggtcctggg cgcccaggac cttgccgccc tgctcaaggc catcgaacag
     1021 ctttcccaag acggagacca gctcagggca atgcaactgc tcgccatgct gcaggagatg
     1081 atcgaaaatc tacaggtcgc gggcggagcc ggcggccccg gcggcggcaa cgcagccgcc
     1141 gatcaggccc tgcgcgacct cagcgagctg atgggcaagc agcgcatgct gctggacaag
     1201 accttccgcc agagccaagg caccggcgat cccaaggacg gcggcgccaa gggcctggcg
     1261 cagcagcaag gccagttgca gaacgatctc ggcgcattgc tcaagaaggg cggcaagagc
     1321 ccatcgcaag gcgatttgca gcgggcggaa aagatgatgg gcgaagccgc tcaggcgctc
     1381 gggctgggcg atcttccccg cgccgggacg ttgcagaaga acatcctcga cgcgctgcgc
     1441 aaaggcgccg aagcgatggc cgccggccgc ggcaagggac aacagggcgc ccaagatccg
     1501 ctcggccgcg cggcggaggg ccgcggcgcg ggcaccggcg gcaatttgcg catcccggat
     1561 gcgtcggtgt tgcaacgcgc gcgcgacatc ctcaacgagc tgcgcaagcg ggctggcgaa
     1621 caaaagcggc cgaaagagga actggactat atcgaccggc tgttgaagca gttctgagaa
     1681 aagcgtgttg gcatctagtg tcgtggtttc gaagtttgct ctatctgcta tatctggctg
     1741 taagcgaact tcgaaaccaa aacgacacta ggattcaata ggttgctagt gtcctttcga
     1801 ttccgaaatt cgcccgaagc cgatatagag atggtgcgaa tttcggaatc gggacactag
     1861 gtgccgcgcg aaaaaccggt gtcacggccc gcctatgtgc gggccaccca agtgacgacg
     1921 gcgcgctgct caaggaattt tcgctgcgtt cttccttgac gatctcaaca ggtttcaact
     1981 ggatggcccg cagttgcggg ccatgacaag cgagttttga gtccgcgcgc tcgtacgtcg
     2041 ctctaaatca gcccagcctt ggcgatgtag ggcagggcgc ggtccttgcc cgcgtcgtcc
     2101 atgccgtagc cgacgatgaa ttccggcacg gccgtgaacg ccgcatagtc ggacacggcc
     2161 accgcatcct tgcgcgcctt gtccaccacc accgccgaac gaacggaggc cgcgcccgcc
     2221 gcaatcgcca gttcgcgcgc cttgtggatc gtgtggccgc cgtcgagcac gccatcgatc
     2281 aagagcacgt tcttgccgcg gaaattctcg ttgggcccga tcaggaccga gatctccttg
     2341 gtcgccgtgc gcccgacata acttctgagc cagagaaact ccgtcggcag ggaaatccct
     2401 tcacgcgcca gcgcccgcaa cagatcgccg gcgaagacat aggcgccgac cagaatcggc
     2461 acggcgatct caggcttgac cggcgccgcg gcgatccgct gcgccatgtc cgtcacgcga
     2521 ccggcaatca cgctctcgga aaacagaatc tcgctgtgca tgtccgatct ttcactcgcc
     2581 ggccctggca aaacgcaggt cgagatggcg cgcgtccgcc ggcggactcg acaggcgggt
     2641 gacgaaaggc gtgctttggc cgggacgcag cgaaatcacc gccggcatga agctccaatg
     2701 gtacagttcg cgcctggctt cgtctttcag cgtcacgcga atctgcggca cggacaattc
     2761 gcttccgctg acgttcacca gcttgccggt caccaccagc atgggttggc cgccttggat
     2821 ttcctcgtga taccggtaat cgacaatccg cagaccgctg ctatccgtct tcatgccgag
     2881 ggtggaatag agcgaagccg attgcggcca cttggccacg atctgctggc gatagacgct
     2941 cgccgtccag ccgatcagaa ccacgacggc ggcaagcgcc gcccagcccg tgaccagcag
     3001 aagacgccgc ggccagagcg aacgcggctt gggcgcttct tgctgccgtt cttgcggctc
     3061 ggcctcgtat gagggcggct gcacagtgat aggcggacgg ggcggggctt cgtaggcgct
     3121 gggagaaggt ccgggcgcca caaaaaccgg ttcgggttcg ccctcagcct cgggtgcggg
     3181 ctgatgccac acatggccgc acttggcgca gcgcacattg cgtccggcgg gcgcgaaatt
     3241 tctttcgtcg gcctggtaac gcgtagagca agctggacag gtgaggatca tcccggcggc
     3301 gatcaggttt ctcgggcgaa aataggcggc aaccgtggcc cagacaaggc tcgtagccgg
     3361 gttccggtcc ggttgcagcg gctgcgtgac ggaattgctc cagtttgtcg atttggccgc
     3421 atttccgcgg ctttacgcag gcaaaaacac tgcatatttc gccgaaaaat gctctaacgt
     3481 tc
//
LOCUS       DASWPU010000160         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_80688,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000160 DASWPU010000000
VERSION     DASWPU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80688"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VGC27_03620"
     CDS             <1..559
                     /locus_tag="VGC27_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0281697.1"
                     /translation="IAAAAVAAGLLWHAGDSFSGANRSAGEASVGGPFTLTDQNGEMR
                     RAEDFRGRFMLVYFGYSYCPDVCPTTLAVMADALDRLGSKAERIVPIFITVDPGRDSP
                     RVLKSYLKAFGPRFVGLTGEAKAVAAAARAYRVYYKERPLQGGGYAMDHSGHIYLMGA
                     DGKFIAHYDETLGPDGLAAALKKAI"
     gene            complement(726..1610)
                     /gene="folD"
                     /locus_tag="VGC27_03625"
     CDS             complement(726..1610)
                     /gene="folD"
                     /locus_tag="VGC27_03625"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF010785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY0281698.1"
                     /translation="MSASIIDGKIHAAALRARLAEATARLKAEHALVPGLATVLVGDD
                     PASEVYVRNKNKTAEALGFKSVHRHLPVAASEAEVLGEVKALNADKSVHGILVQLPLP
                     GQIREESVLNLLDPAKDVDALTPTNAGLLLSGRAKLVSCTPMGVMILLKETLGEITGL
                     DALVIGRSLLFGKPAAQLLLAANATVTMAHSKTRDLPQLARRADILVAAIGRPHYVKG
                     DWVKPGATVIDVGINRVDAGEGKTRLVGDVDYEEAAKVARFITPVPGGVGAMTIVCLM
                     RNTLIAAASQAGLPIPKV"
     gene            complement(1607..1918)
                     /locus_tag="VGC27_03630"
     CDS             complement(1607..1918)
                     /locus_tag="VGC27_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEY0281699.1"
                     /translation="MRVRAGERGVSLQVRLTPKGGRDAVEGWAEGAGGISYLKARVRA
                     APEDGKANAALLELLAETLSVPKSALRIAAGGSARLKRIEIAGDGALLAARLEALGDA
                     G"
     gene            complement(1930..2223)
                     /locus_tag="VGC27_03635"
     CDS             complement(1930..2223)
                     /locus_tag="VGC27_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY0281700.1"
                     /translation="MIDPISSLILLLLELYWWIVIVAVVASWLIAFNVINLHNNIVRS
                     VVHLLDALTQPVFRQVRRVIPVFAGIDISPLFVLIGIWFLQKLVIWVAWRLAV"
     gene            2384..2459
                     /locus_tag="VGC27_03640"
     tRNA            2384..2459
                     /locus_tag="VGC27_03640"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2417..2419,aa:Ala,seq:cgc)
     gene            2718..3128
                     /locus_tag="VGC27_03645"
     CDS             2718..3128
                     /locus_tag="VGC27_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281701.1"
                     /translation="MRRSLLPACLAVLALAVPAGAAKTATVHNTQSSSYLMIDPIYTT
                     IMDGNRIAGLMMVGIGLDVPDGDLRAQAKRSMPVLRDVYVRSLMAYTATSVRPWRQPD
                     VGEIADRLQRVTDRTLKRKGARILLAQVAMRLKK"
     gene            3231..>3755
                     /locus_tag="VGC27_03650"
     CDS             3231..>3755
                     /locus_tag="VGC27_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0281702.1"
                     /translation="MSEPLIAADRLSFHAGGAHILDRVSLAVHPREIVTLIGPNGSGK
                     TTLLKAILGLVRAEGKVSRQPGLKIGYVPQHFERDASLPLTVSGFLGLFAPAKAAASA
                     LARVGIAEMKTRQLGALSGGELARVLLARAIAARPDLLVLDEPITGVDVAGEAALYHL
                     IAEIRDEIGCGVLLV"
BASE COUNT          640 a         1236 c         1194 g          685 t
ORIGIN      
        1 tatcgcggcg gccgcggttg cggcgggtct tttgtggcat gcgggcgatt cattttcggg
       61 cgccaaccgg agcgcgggcg aagcctcggt cggcggaccg ttcaccttga ccgatcagaa
      121 cggcgaaatg cgccgcgcgg aggatttccg cggccgtttc atgctggtct atttcggcta
      181 cagctattgt cccgatgtct gccccacaac tctggcggtg atggccgacg ccctcgaccg
      241 gctgggaagc aaagccgagc gtatcgtacc catcttcatc accgtcgatc ccggccgcga
      301 ctcgccccgc gtcctgaaat cctatttgaa agcgttcggg ccgcgttttg ttggcctcac
      361 cggcgaggcg aaagccgtcg cggcggcggc gcgcgcctat cgcgtctatt acaaggagcg
      421 gcccctgcaa ggcggcggct acgccatgga ccattccggc catatctatc tgatgggagc
      481 ggacggaaaa ttcatcgccc attacgacga aaccctcggc cccgacggct tggcagcagc
      541 attgaagaag gcgatctagg tgtttagtcc cggcaattgg tggattggat tgagccgatt
      601 ctcctttgct caccatggcc gggcttagtg ttcagcccgg ccatccagtt gctggatggg
      661 cggctcgacg gccgcccatg gtgaggcggt ttaccaagtg tgcttcatcc ttatccaaaa
      721 ccaatttaaa ccttcggtat cggcaatccg gcttgcgacg ccgccgcgat cagcgtgttg
      781 cgcatcaaac agacgatggt catggcgccg acgccgcccg gcaccggggt gatgaagcgc
      841 gcgactttgg cggcctcttc gtaatccaca tcgccgacca gcctggtctt gccctcgccc
      901 gcatcgacgc gattgatgcc gacatcgatc accgtcgccc ccggcttgac ccaatcgccc
      961 ttgacataat gaggccggcc gatggccgcg accaggatgt cggcgcggcg cgcaagctgc
     1021 ggaagatcgc gcgtcttcga atgcgccatg gtgacggtgg cgttcgccgc caatagaagc
     1081 tgcgccgcgg gcttgccgaa caacagcgag cggccgatga ccagcgcgtc cagccccgtg
     1141 atttcgccca gcgtttcctt gagcaggatc atcaccccca tcggcgtgca ggacaccagc
     1201 ttggcgcggc cgctgagcag gagcccggcg ttggtgggcg tcagcgcgtc gacgtccttg
     1261 gcgggatcga gcaggttgag caccgactct tcgcgaattt gtcccggcag cggcaattgc
     1321 actaatattc cgtgtacgga cttatcggcg ttgagcgcct ttacttcgcc gagcacttcg
     1381 gcctcgctcg cggcgacggg cagatggcgg tgtacggatt tgaagccgag cgcctcagcg
     1441 gtcttgttct tgttgcggac atagacttcg ctggccggat cgtcgccgac caggaccgtg
     1501 gccaatcctg ggaccagagc gtgctcggct ttcagccgcg ccgttgcttc ggcgaggcgc
     1561 gcgcgcaacg ccgccgcatg aatcttgccg tcgatgatgg aagcgctcac cctgcatccc
     1621 ccaaagcttc gaggcgggcc gccagaaggg cgccgtcgcc ggcgatttcg atccgtttca
     1681 gccgcgccga gccgccggcc gcgattcgca gcgcagactt gggcaccgac aaggtttccg
     1741 caagaagctc caacagcgcg gcattggcct tgccgtcttc gggcgccgcg cgcacccgcg
     1801 ccttcaggta ggaaatgccg ccggcgccct cggcccagcc ttccaccgca tcgcgcccgc
     1861 ccttgggggt caggcgcact tgcaggctca cgccccgttc gccagcgcgg acgcgcaccg
     1921 gcacggccgc tagaccgcca gccgccaagc gacccagatg accagtttct gcaggaacca
     1981 gatgccgatc agcacgaaca agggcgagat gtcgatgccg gcaaagaccg ggatgacgcg
     2041 tcgcacctgg cggaagacgg gctgggtgag ggcatcgagc agatgcacca cgctgcgcac
     2101 gatattgttg tggaggttga tcacattgaa ggcgatgagc cagctggcca ccaccgccac
     2161 gatcacaatc caccagtaga gttcgagcag cagaagtatc agcgacgaga tgggatcgat
     2221 catcggggtc tggcgctccg gggcggggtt gagaatgcga gccgttttgc cagtccaggc
     2281 gccccgccgc aaggcgcatg ccttgacagg tttggggggc gctccctaga taccgccccc
     2341 gcgcccggct ttctcttgcc gcgccgggca ccaagcttgg cttggggctg tagctcagtt
     2401 gggagagcgc ctcgttcgca atgaggaggc cagcggttcg attccgctca gctccaccac
     2461 ccttcgctgc tgcgcagctt tgggtggcag gctacccaat tctcccacag cgaagggtgt
     2521 cctccgaagc cgtccatagc gccagcgtat ggacgggcga agacgggcta gttgccgcga
     2581 actacgcagg caagcctttg tttctcctca tttttctctg gatgccatcc accgcaccct
     2641 ctttccggcg agaccggcgc taaccatcgc tttaccaaac tggattaaat ccgggaggac
     2701 cagcgaaaga ccgacccatg cgccgttccc ttctgccggc ctgtcttgcc gttctggcgc
     2761 tcgcggtgcc ggccggtgcc gccaagacgg ccaccgtcca caacacccaa tcgtccagct
     2821 atctgatgat cgatccgatc tatacgacga tcatggacgg caaccggatc gcgggattga
     2881 tgatggtcgg catcggcttg gacgtgcccg acggcgatct gcgcgcccag gcgaagcgtt
     2941 cgatgccggt gctgcgagac gtctatgtcc gtagcctgat ggcgtatacc gcgacctcgg
     3001 tgagaccctg gcgtcagcct gacgtgggag agatcgcaga ccggctgcaa cgcgtcaccg
     3061 accgtacctt gaagcgcaag ggcgcgcgca tcctgctcgc ccaggtggcc atgcggctga
     3121 agaaatagcc ggcggtcgcg ccggcggcgc cttcgctcga gcgttcggga ccaggaaacg
     3181 gcctgatccc cctgtttctt ccgaaaagcg caatgttata acggcaggcg atgagcgaac
     3241 ctctgatcgc ggccgaccgg ctgagcttcc atgccggcgg cgcgcacatc ctcgaccgcg
     3301 tgtccctggc ggtccatccg cgcgagattg tcaccctgat cgggccgaac ggctcgggca
     3361 agacgacgtt gctgaaggcc attctcggcc tggtgcgggc cgagggtaaa gtcagccgcc
     3421 agcccggcct caagatcggc tatgtgcccc agcacttcga gcgcgacgcc agcctgccgc
     3481 tgacggtttc aggctttctc ggccttttcg cgccggccaa ggcggcagct tccgctctgg
     3541 cgcgggtcgg catcgccgag atgaaaaccc ggcaattggg ggcgctttcg ggcggcgaac
     3601 tggcccgggt gctgctggcg cgcgccatcg ccgcccggcc ggacctgctg gtgctcgacg
     3661 agccgataac cggggtcgat gtcgccggcg aggccgcact ctaccatctg atcgccgaaa
     3721 tccgcgacga gatcggctgc ggtgtgcttc tggtc
//
LOCUS       DASWPU010000161         1809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_111504,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000161 DASWPU010000000
VERSION     DASWPU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111504"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VGC27_03655"
     CDS             <1..588
                     /locus_tag="VGC27_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281703.1"
                     /translation="YTGRMTRSTPKILAAVLTLLVIGGFAYWGLKPLVQPPAVVAMPP
                     SAIKHPVKPVEVPPGWVKIDTKQGFTFYAPPGTQFSPLEGEDSFLGEITGPDFGLQSD
                     FGFWSNDLSDARNEQDYSEEQVVIDGRAGVIRRATFASEDGPPSYFAGLYVQQAVFHR
                     EYPGRWAALEIHGSARTPQDRATVERMLKTVQFGE"
     gene            672..968
                     /locus_tag="VGC27_03660"
     CDS             672..968
                     /locus_tag="VGC27_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY0281704.1"
                     /translation="MSRPSGFVYILASKRNGTLYIGITSDLLGRVQAHREGRGSEFCK
                     KYNVTHLVYFEEYPLYADAIRRETNLKRWKRAWKIALIEKVNPNWDDLIEKWHA"
     gene            1373..>1809
                     /locus_tag="VGC27_03665"
     CDS             1373..>1809
                     /locus_tag="VGC27_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEY0281705.1"
                     /translation="MFDSLQTRLGGVFDKLRGRGALSESDVDAALAEVRTALVEADVA
                     LGVIDDFIAKVRPRAIGENVIRSVQPGQQVVKIVHDQLVETLGSDNAALNLGSAPAPV
                     LMVGLQGSGKTTTSAKLAMLLQNKEKKRVLMASLDVNRPAAME"
BASE COUNT          346 a          576 c          539 g          348 t
ORIGIN      
        1 tatactggcc gcatgacgcg atccactccg aagatccttg ccgcggtcct gacgctcctc
       61 gtcatcggcg gtttcgccta ttgggggctc aagcctttgg tgcaaccgcc tgccgtggtg
      121 gccatgccgc cgtccgcgat caagcacccg gtgaagccgg tcgaagttcc tcccggctgg
      181 gtgaagatcg acaccaagca gggcttcacc ttctacgccc cgcccggcac gcaattcagc
      241 ccgctcgaag gagaagattc gttcctcggc gaaatcaccg gcccggattt cggccttcag
      301 tccgatttcg gcttctggtc gaacgatctc agcgacgcca ggaacgagca ggattattcg
      361 gaagagcagg tggtcatcga cggccgcgcc ggcgtgatcc ggcgcgcgac gtttgcctcc
      421 gaggacggac caccgtccta cttcgccggc ctttacgtcc agcaggcggt ttttcaccgc
      481 gaatatccgg gccgctgggc cgcgctggag attcacggct cggcgcgaac cccgcaggac
      541 cgggcgaccg tcgaacggat gctcaagacg gtccaattcg gcgaatagaa agtcgaaaga
      601 gcaataagta attatataac gctcaccatg gccgggcttg acccggccat ccagcttttg
      661 acaatcgcgc catgtccagg ccgagcgggt ttgtctacat tcttgccagt aagcgcaacg
      721 gcacgcttta catcggcatt acgtccgatc tgctcggccg agtacaggct catcgcgaag
      781 gccgcggctc ggaattctgc aagaagtaca atgtgacgca tttggtctat tttgaggaat
      841 atcctctcta tgccgatgcc attcgtcgcg agacaaatct caagcgctgg aagcgcgcct
      901 ggaaaattgc tctgatcgag aaggtcaatc ccaattggga cgacttgatc gaaaagtggc
      961 acgcctaacg cgctacaaga atttctcacc atgggcggcc ttcgagccgc ccatccagga
     1021 cccggaccag acgcttgccg catggatggc cgggtcaagc ccggccatgg tgagatttgg
     1081 tatttattat ggcggaactt gtttcgccct ttgcggcaag agaggcacat ttgacttgcc
     1141 cgccgcctca tcctaaaacc cagccgcttc cggaaagagc ttaggccttt ccggtcccgg
     1201 gccttgcgcc tgggatcgcc cccaggcagc gcagatgcgc cccctggggc cgttggtgtt
     1261 tggttttacc ctcgcccttc gacaagctca gggtgagggg tcggcttatc cctcatgctg
     1321 agcccctcat cccgagcttg tcgagggacg aagcatgagg ggaaggattt gaatgttcga
     1381 ctctctgcaa acccgcctcg gcggtgtgtt cgacaagctg cgcggccgcg gcgcgctgtc
     1441 ggaatccgac gtcgatgcgg cgctggctga agtgcgcacc gcgctggtgg aagccgacgt
     1501 cgcgctcggc gtcatcgacg atttcatcgc caaggtgcgc ccgcgcgcca tcggcgagaa
     1561 cgtcatccgc tcggtccagc ccggccagca ggtggtcaag atcgtccacg accagctggt
     1621 cgaaacgctc ggcagcgaca acgccgcgct gaacctgggc agcgcccccg cgccggtcct
     1681 gatggtcggc ctgcaaggtt cgggcaagac cacgacctcc gccaagctcg ccatgctgct
     1741 tcagaacaag gagaagaagc gcgtgctgat ggcctcgctg gacgtcaacc gtcccgccgc
     1801 catggagca
//
LOCUS       DASWPU010000162         6752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_99272,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000162 DASWPU010000000
VERSION     DASWPU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99272"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VGC27_03670"
     CDS             complement(<1..273)
                     /locus_tag="VGC27_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281706.1"
                     /translation="MAQYQPFVPFGRSHEIVLALTVLVPILLALLVRATASRVLTRAI
                     CRIFAAAVLGAWAYWFWISTRNGWLDLGNGLPMDLCSWACIATVITL"
     gene            422..1864
                     /gene="zwf"
                     /locus_tag="VGC27_03675"
     CDS             422..1864
                     /gene="zwf"
                     /locus_tag="VGC27_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY0281707.1"
                     /translation="MTDQIAIVIFGATGDLAQKMLYPSLYFLCAEGHLPGGLAVGGFS
                     HTDVSEADFIAKTRKAVKETAGKDFAEAAWKRFAARLRYCKGNLNTAKDYAPLAKLLG
                     SGEVVFYLATSPVFFGTIARNLKEAGLAHQGARIAIEKPVGHDLISCRKIECELADAF
                     SEDRIFRVDHYLGKEPVQNLIALRFANTMFEPLWNKNSIEHVQITIAETLGVERRWSY
                     YDSYGALRDIVQNHLLQLVCLVAMEPPASLDPDTVRDEKVKVLRSLRRIGSKDVERQT
                     VRGQYGKGFSGEATVPGYSEEQGAAASDTETFVAIAAHIDNWRWAGVPFYLRTGKRMA
                     MRHTQIVVQFRQVPHSIFPHHDLIANRLTIRLQPEEGISLQLMNKTPSLDRGGMRLKP
                     LSLDLSLSDAFKRQRQRIAYERLFLEALEGNSILFVRRDEVEESWKWIDAIIEGWKER
                     EMKSAPYTAGTWGPSAAFALIERNGNSWCE"
     gene            complement(2018..2698)
                     /gene="msrA"
                     /locus_tag="VGC27_03680"
     CDS             complement(2018..2698)
                     /gene="msrA"
                     /locus_tag="VGC27_03680"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY0281708.1"
                     /translation="MNRIIGPVAALLVLFAGSACANEAASALPAPALDSKADPSRTTE
                     TAVLAGGCFWGMQGVFEHVKGVKQVLAGYAGGTASTAHYEMVGNGRTGHAEAVEIVFD
                     PRIVSYGQLLRVFFSVMDPTTLNYQEPDRGTQYRSEIFTANPEQQRVAAAYIAQLEKA
                     RAFPAPIVTRLGALHGFYRAEDYHQDFLILHPDDAYIVINDLPKIATLKRLYPQLYTD
                     RPVRVAPK"
     gene            complement(2710..3111)
                     /gene="msrB"
                     /locus_tag="VGC27_03685"
     CDS             complement(2710..3111)
                     /gene="msrB"
                     /locus_tag="VGC27_03685"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0281709.1"
                     /translation="MEKIVKTDAEWKTLLTPGQYQVARRDGTEPAYTGDTWDNHADGL
                     YRCVCCGTPLFDSKTKFESGTGWPSFWRPIDKANIVETRDRSLFMDRVAVSCARCDAH
                     LGHVFDDGPKPTGLRYCMNSASLKFVPRAPA"
     gene            complement(3343..4422)
                     /locus_tag="VGC27_03690"
     CDS             complement(3343..4422)
                     /locus_tag="VGC27_03690"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HEY0281710.1"
                     /translation="MIAWLQALKPYERHLSAAAMVGGYGFDSIAYGRVDHPVTQTLLL
                     VYLLVAGGSIMLLHYLQAHVEWEGFAAKLRSFLPMLTQFAFGSLWSAFLVFYARSGVL
                     ASSWPFLVVLACIFLGNEIFRAYHSRLIFTSILFFFALQSYAIFMVPVFTHTIGTATF
                     VASGLAAAFVFLLFVRTLNRLSAARLGEVRWQMLSGAALVFAAVNGLYFLSVLPPLPL
                     AMQQAGVFHSVKRLGPVYYAMTESQHWSVYLGVPPTMHVERGKPVYVFSAVFAPIKLS
                     TTVVHHWQRYDDVKGKWTAVQSVPYVITGGRGNGYRGYTKKTGPKPGLWRVDVNTVDG
                     RLIGRIEFMVVRGPANAANVTTVMS"
     gene            complement(4475..4846)
                     /locus_tag="VGC27_03695"
     CDS             complement(4475..4846)
                     /locus_tag="VGC27_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY0281711.1"
                     /translation="MAGKANKTTRPGPFMKLARPEMIEAVALAGFDFALVDVEHGPVS
                     QSELYAVVMAAERRNLPLVVRVPVREEPYIKWCLDPGVRHIQVPSVETAEDAAFGVRN
                     SYFNPPGMRGLCRFTRAAAFP"
     gene            complement(5001..>6752)
                     /locus_tag="VGC27_03700"
     CDS             complement(5001..>6752)
                     /locus_tag="VGC27_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281712.1"
                     /translation="EWWIDNRQVPYGDFGGGISDDDDLTQQFPPLALMGDIPDKVRAS
                     LHALADAVDRNGMITNGLGTIETDYLHSYEEGLNARSQDMYLNWGDPKVVERLMQTAA
                     AYPRITELNKEGHTHIVSQLFSGTHVVREGPWGWSHPYSYLILHPGIMLAEFNGNPAM
                     KKLALALADSYIAHGKQAADGSWRFPEFINSGTDETSGTLKPNTRGINAATQLFWTAY
                     AWSGDKKYLLPLIGVIDPGLHGGLRFMNANLIGQLGKAESWGKDILAHMRKLRDAAAG
                     ETRGFSPARDDGATPVNTGRYIAWLMSGDKKYLEDLYADEIQTGSQRMYMVTEGHWWS
                     DRVELFSDLLQRSRLGGLALRRNQIYPGHLVSWRFGAPTEAESVGILIHDPLPTKFTV
                     EAYNLSAKPIEAAMTGWMIAPGTWKMSGKGIETRTFTFERSAGVNLSFAPHKTTLVNF
                     ELVTPGPSTAVRPDIGIGPDDVELEAGKLAVTVHSLGAMDTPAGTAMLMDAKGKTLAS
                     VAVPPLAAPLDLEPKTARVELPLKPGAARVRIILDGGIPEITQLNNEVRVAPERVTKP
                     VKPAKPVKRSQRQRKAL"
BASE COUNT         1303 a         2145 c         2087 g         1217 t
ORIGIN      
        1 cagcgtgatc accgtggcga tgcaggccca actgcacaaa tccatcggca atccgttgcc
       61 aagatccagc cagccgttgc gcgtggagat ccagaaccaa taggcccagg cgccgaggac
      121 ggcggcggcg aaaatccggc aaatcgcgcg cgtcagcacg cggctcgccg tggcgcgaac
      181 cagcagcgcc aacagaatcg gcaccaagac cgtgagcgcc agcacgatct cgtgcgaccg
      241 cccgaacggg acgaagggtt gatattgcgc catgatcccc cttcctccac gaagagccgc
      301 attctacgcc aaaagaagaa ggggcgtgag acgcccccta tcactttcgc cgcggtttcg
      361 cgtaacttgt catggaaccg tgccggcttt gccggcgggc gcggccagac gggaccggag
      421 aatgacagac cagatagcaa tcgtgatctt cggggcgacg ggcgatctgg cgcagaagat
      481 gctctacccc tcgctctatt tcctctgcgc cgaagggcat ctgccgggcg ggcttgccgt
      541 cggcgggttc tcgcataccg acgtgagcga agccgatttc atcgccaaga cgcgcaaagc
      601 ggtcaaggag acggccggca aggatttcgc agaagccgcg tggaagcggt tcgccgcccg
      661 cctgcgctat tgcaagggca atctgaacac cgcgaaggac tacgcgcccc tggccaagct
      721 gctgggcagc ggcgaggtcg tcttctacct ggccacctcg cccgtctttt tcgggaccat
      781 cgcccgcaat ctgaaggagg cggggctcgc ccaccaaggc gcgcggatcg ccatcgaaaa
      841 accggtcggc cacgatctga tctcctgccg caagatcgag tgcgagcttg ccgacgcctt
      901 cagcgaagac cgcatcttcc gggtcgatca ttacctcggc aaggagccgg tgcagaacct
      961 gatcgcgctg cgcttcgcca acacgatgtt cgagcccttg tggaacaaga actccatcga
     1021 gcatgtgcag atcaccatcg ccgagacgct cggcgtggaa cggcgctggt cctattacga
     1081 ttcctacggc gcgctgcgcg acatcgtgca gaaccacctg ctgcagcttg tctgcctggt
     1141 ggcgatggag ccgccggcaa gcctcgatcc cgacacggtg cgcgacgaaa aggtgaaggt
     1201 gctgcgttcg ctgcgccgca tcggcagcaa agacgtggaa cgccagaccg tgcgcggcca
     1261 atacggcaag ggcttttccg gcgaagccac cgtgcccggc tacagcgagg aacagggcgc
     1321 cgccgccagc gataccgaga ccttcgtggc catcgccgct catatcgaca attggcgctg
     1381 ggccggtgtg cccttctacc tgcgcaccgg caagcgcatg gcgatgcgcc acacccagat
     1441 cgtcgtgcag ttccgccaag tgccgcattc catcttcccg caccacgatc tgatcgccaa
     1501 ccggctgacc atccgtttgc agccggaaga aggaatttcg ctgcagctta tgaacaagac
     1561 tcccagcctc gaccgcggcg gcatgcggct gaagccgctg tcgcttgatt tgagcctgag
     1621 cgacgccttc aaacgccagc gccagcgcat cgcttacgag cggctgtttc tggaagccct
     1681 ggaaggcaac agcattctgt tcgtgcgccg cgacgaggtg gaggagtcct ggaaatggat
     1741 cgacgccatc atcgaaggct ggaaagagcg cgaaatgaaa tccgcgccct ataccgcagg
     1801 cacttgggga ccgtcggccg ccttcgcgct catcgagcgc aacggcaaca gttggtgcga
     1861 gtgagggaat ctgatctctt ggctctgctc tgccccttcg acaagctcac ccttcgacaa
     1921 gctcagggtg aagggagcat tttacccctc atgctgagct tgtcgaagca tgaaaccgcc
     1981 gcgatctcat atctgtgaat agcgccctgg cccgttccta tttcggcgcc accctgaccg
     2041 ggcggtcggt gtaaagctgc ggataaagcc gcttcagcgt cgcaatcttc ggcaggtcgt
     2101 tgatcacgat ataggcgtcg tcgggatgca gaataagaaa atcctgatgg tagtcctccg
     2161 cccggtaaaa cccgtgcagc gcgccgagcc gggtgacgat gggcgccggg aaggcgcgcg
     2221 ccttttccag ttgcgcaata taggccgcgg ccacgcgctg ctgttctgga ttggcggtga
     2281 aaatctcgga acggtactgc gttccccgat cgggctcctg atagttcagc gtcgtcgggt
     2341 ccattacgga gaagaaaacg cgcaggagct ggccgtagga gacgatcctc ggatcgaaaa
     2401 cgatctcgac cgcttcggcg tggccggttc ggccgttgcc gaccatttcg taatgcgccg
     2461 tcgaggcggt gccgcccgca taaccggcca gcacctgctt gacgcccttc acatgctcga
     2521 aaacgccctg catgccccag aaacagccgc ccgccagaac cgcggtttcc gtcgtccgcg
     2581 acggatcggc cttactgtcg agcgcaggcg cgggcaaggc gctcgcggcc tcgttagcgc
     2641 aagccgagcc ggcgaacaaa accagcagcg cggcaacggg gccaataatc cggttcatcg
     2701 cgttctcctt caggcggggg cgcgcggcac gaatttgagc gaggcggaat tcatgcaata
     2761 gcgcaggccg gtcggcttgg gaccgtcgtc gaagacgtgg cccagatgcg catcgcagcg
     2821 ggcgcaggaa accgccacgc ggtccatgaa caagctgcgg tcgcgcgttt ccacaatgtt
     2881 ggccttgtcg atcggccgcc aaaagctggg ccagccggtg ccggattcga atttcgtttt
     2941 cgagtcgaac aacggcgtgc cgcaacagac gcagcgatag agaccgtcgg catggttgtc
     3001 ccaggtgtcg ccggtgtacg ccggttcggt gccgtcgcgg cgcgccacct gatattgccc
     3061 aggtgtcagc agcgtcttcc attccgcgtc ggttttgacg attttttcca tctgtaccgt
     3121 tcccagtttc tttcccgaag ccgaaaactc cgcgatcgca accgtgcgcg gcgcgcccgc
     3181 gcgcgccggc aaagaccaca ataaaaatgg ggcgaatgac gaatatgcca gaattgtgcg
     3241 gcgggtttga gctgtcacgg caatctctgc gatccggctt cctaagagcg atatgggtag
     3301 ttcgctccag gatacaagat cgttgcggag gccgcgcgcg cttcagctca tgaccgtggt
     3361 cacgttcgcg gcgttggcgg gcccgcgcac caccatgaat tcgatgcgcc cgatcagccg
     3421 cccgtcgacc gtattgacgt ccacccgcca caggcctggc ttcgggccgg ttttcttggt
     3481 gtagccgcga taaccattac cgcgcccgcc ggtgatgaca taaggcaccg actgcaccgc
     3541 agtccatttg cccttaacat cgtcgtaacg ctgccagtga tggacgaccg tggtcgacag
     3601 cttgatcggc gcgaacaccg cactgaatac gtaaaccggt ttgccgcgct ccacatgcat
     3661 tgtcggcgga acgccgagat agacgctcca gtgctggctc tcggtcatcg cgtaatagac
     3721 cgggccgagc cgcttgacgg aatgaaagac gccggcctgc tgcatcgcca gcggcaaggg
     3781 cggcagcacg ctgagaaaat agagtccgtt gaccgcggcg aacaccaacg ccgcgccgct
     3841 cagcatctgc catcgcacct cgcccagccg cgccgcgctc agccggttca gcgttcgcac
     3901 aaacaacagg aagacgaacg ccgcggccaa gccgctggcc acgaaagtcg ccgtgccgat
     3961 ggtatgcgtg aataccggca ccatgaagat cgcataggac tgaagcgcga aaaaaaacag
     4021 tatggaggtg aagatcaagc gcgaatgata agcgcggaaa atctcgttgc cgaggaagat
     4081 gcaggccagg accaccagaa acggccagga cgaggccagc acgccgcttc tggcgtagaa
     4141 caccagaaag gcgctccaca ggctgccgaa ggcgaactgc gtcagcatcg gcagaaagct
     4201 gcgcagcttg gcggcgaagc cttcccattc gacatgcgcc tgcagatagt gcagcagcat
     4261 gatcgagccg cccgccacca gcagatagac gagcagcaag gtctgggtga cgggatgatc
     4321 cacccgtcca taggcgatgc tgtcgaagcc gtagcccccg accatcgccg cagccgacag
     4381 atggcgctcg taaggtttca gcgcttgcaa ccaggcaatc attgttcggt cagccgcagc
     4441 aaaaacatcg tggaataaga cttgtccttg ccggtcatgg gaaggcggcg gcacgcgtga
     4501 agcggcatag cccgcgcatg cccggcggat tgaaatagga attgcggacg ccaaaggcgg
     4561 cgtcttccgc cgtttccacc gacggcacct gaatgtggcg gacgccggga tcgagacacc
     4621 atttgatata aggctcctcg cggacgggta cacgcacgac cagcggcaga ttgcgccgtt
     4681 cggccgccat taccacggca tagagttcgc tctggctgac cgggccgtgc tccacgtcca
     4741 ccaaagcgaa atcgaagccg gccagcgcaa ccgcctcgat catctcgggg cgcgccagct
     4801 tcataaaggg tccaggccgc gtcgtcttgt tcgccttgcc cgccatgtgc atgcctttgg
     4861 atgttcggga accgttgggg cgggcggacc ctagcctcgg cagacccgcc aagcaagttt
     4921 ccgccgctgt cccggcaccg cctgccgctc ccaaaagtgc tgattttatt gagcgatgcg
     4981 catgggccgg tgcaggcgcg tcatagcgct tttcgttggc gctgcgagcg cttgaccggc
     5041 tttgccggtt tcaccggctt ggtcacgcgc tctggtgcaa cacgcacctc attgttcagt
     5101 tgcgtgatct ccgggatgcc accatcgagg atgatgcgga cgcgcgcggc gcccggtttc
     5161 agcggcaact cgacccgcgc cgttttgggc tcgaggtcga ggggcgcggc gaggggcggt
     5221 acggcaacgc tggccagcgt cttgcccttt gcatccatca gcatggccgt gcccgccgga
     5281 gtgtccatgg caccaaggct atgaacagtg accgccagct tgccggcttc gagttcgacg
     5341 tcatcggggc cgatgccgat gtcggggcgg acggccgtcg aaggcccggg tgtcacaagc
     5401 tcgaagttca ccagcgtcgt cttatgcggc gcaaaggaga ggttcacgcc ggccgagcgt
     5461 tcaaaggtga aggttcgggt ctcgatgccc ttgcccgaca ttttccacgt ccctggcgcg
     5521 atcatccagc ccgtcatcgc cgcttcgatc ggcttggcgg acaggttgta ggcctccacg
     5581 gtgaatttcg taggcagcgg atcgtggatc agaatgccga cgctttccgc ctcggtgggg
     5641 gcgccgaacc gccagctcac caaatggccc ggatagatct ggttgcgcct gagcgccaag
     5701 ccgcccagcc gcgaacgctg gagcagatcg ctgaacaatt ccacgcggtc gctccaccaa
     5761 tggccttcgg tgaccatgta catccgttga ctgccggtct ggatttcgtc ggcataaagg
     5821 tcttcgagat atttcttgtc gccgctcatc agccaggcga tgtagcgccc tgtgttcacc
     5881 ggcgtcgccc cgtcgtcgcg cgccggcgag aagccgcgcg tttctcctgc cgcggcgtcc
     5941 cgcagcttcc gcatgtgagc gagaatatcc ttgccccagc tttcggcctt gccgagttgg
     6001 ccgatcaggt tggcgttcat gaacctcaga ccgccatgca ggccgggatc gatgacgccg
     6061 atgagcggca acagatattt cttgtcgccg ctccaggcat acgccgtcca gaacaactgc
     6121 gtcgccgcgt tgatgccgcg cgtgttcggt ttcagcgttc cgctggtttc gtcggtcccg
     6181 gaattgatga actccgggaa ccgccagctt ccgtccgccg cctgcttgcc gtgggcgata
     6241 taggaatcgg cgagcgcgag cgccagcttc ttcatcgccg gattgccgtt gaactccgcc
     6301 agcatgatgc cggggtgaag gatcaggtac gaataaggat gcgaccagcc ccacggccct
     6361 tcgcgcacga catgggtgcc gctgaagagc tggctgacga tgtgggtgtg gccttccttg
     6421 ttcagctccg tgatccgcgg ataggcggcg gccgtctgca tcagccgctc caccaccttg
     6481 ggatcgcccc agttcaaata catgtcctgg ctgcgggcgt tgagaccttc ttcgtaggaa
     6541 tgcagatagt cggtctcgat ggtgccgaga ccgtttgtga tcatgccgtt cctatccacg
     6601 gcgtcggcca gcgcatgcag cgaggcgcgc accttgtccg ggatgtcgcc catcagcgcc
     6661 agcggcggga attgctgggt caggtcgtcg tcgtcggaga tgccgccgcc gaaatcgcca
     6721 taaggcacct ggcggttgtc gatccaccat tc
//
LOCUS       DASWPU010000163         6944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_44369,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000163 DASWPU010000000
VERSION     DASWPU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44369"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VGC27_03705"
     CDS             complement(<1..279)
                     /locus_tag="VGC27_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281713.1"
                     /translation="MLFRHRLAAVAFAAAGFFCAVVAADFAFPPDMTNVQDVSPEVVD
                     HHGVLLRAFLSRGGYWRMKTGVSEVSPRYLAMLKAYEDKRFDGHFGVDP"
     gene            complement(371..5278)
                     /locus_tag="VGC27_03710"
     CDS             complement(371..5278)
                     /locus_tag="VGC27_03710"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HEY0281714.1"
                     /translation="MGKYRHWILIGGGVGVAVVLAVVVTLYVLGGRGTGGSGFFDRLA
                     GGLSTVTSAVAPPAAEAGGEFVFRRVDIDTSKDRPEACIVFTRALDPSGHTRYEDYFS
                     IDPATRVAVRVVDQRLCLSGLDFNKIYNVTLKAGLPSASGDKLVESETVPVELRDKPS
                     IVRFNGGIILPRDNAQGVPVTTVNVAKIKLKLIRVGDRLLSQIESGTVDETTLYSWSA
                     NEIQNNQGSLVWQGTMDVANVKNDSVVTLIPIHKVLKKRKPGAYVLIASDASKKGGDD
                     SDEGFSSGEMAVQWVVDSDIALTTFTGAGGLTVFARSYASAKPLEGVKLSLVARNNNE
                     VATAKTNSDGRADFDAGLMRAKGGDEPVVVMAYGNDQDFSFTDLRRSSFDLTDRGVSG
                     RNVPGPVDAYLYTERGVYRSGETVYLTALLRDRIGNAATAPLTLVATRPDGLEVGRVT
                     LPGAALKAGAATWTLPLSSSAPHGRWQIAAFIDTSKDATAIGRVQFDVQDFVPQKLKV
                     TLSAETKVARQNSEIKVRAESRFLYGAPASGLSGEAVARISVDPTPFDNYAGWQFGRV
                     DDSFSDIDVQMTVPPTDANGSTEAAGTIGDLADTTLALRAMVKVSIHEPGGRVTAKTV
                     EIPVRNHDVFIGIRPQFSGASVAEGARAGFEAVALDAGGKRIALSGVTVSWVREDTSY
                     QWYQDNGGWKYKSVTRDRLITSSTMSIGTGASLKLAQAMGWGTYRLTLTDPKSGASSS
                     TRYYSGWAASSAGDRPDRIPVAADKSAYRAGETAHVRIKPAADGKALVVVAGDKVFSS
                     QVVDAPAGGATIDVPVSADWGAGAYVLVTDYRPLDQASGREPVRSIGLVWLPLDNSKR
                     TLATLIGGPKKILPRQKLNIPVQVKGLSRGEEAYLTLAAVDEGILQLTEFKSPDPVSY
                     YYGKRRLGVEMRDDYGRLIKAEKGAVGAMREGGDMFGGRALAVVPTRTVALFSGLVKI
                     GAGGMVSVPLDVPDFNGELRLMAVVFSAGKLGHADRPLTVRDPVVAEIVLPRFLAPGD
                     RAKAALNLNNVEGRPGSYTATVTAGGPVGLAGGAQTVVTQNLSAGQRVLVPVEVRGTG
                     LGIANIALKLTGPDGFSVTRSWPIEVRSPQLDIARDDTVPFAAGTSFTANKALIADLV
                     PGTQTVGLTVSAAHGYSNVPGLLKWLDKYPYGCVEQIASRAMPLLFFNDLADLAGLPK
                     DKALREREQQAVDSVLDMQNFAGDFGMWGPGSDAEPWISAFALDFLMQAKDKGYIVPN
                     DALRRGAAWLKRISASGSFNDNSRAYAFYLLARSGQANVSDLRYFSDTRGPEWNTAIA
                     AALTGSAAAAVGDRSRAAYGFNRAREIIAKAKPNTYPAGDYGSLLRDIAGTIALAAEN
                     GQGQLVPALLSKSEEMKLDMRLNWTTTQEKAWMLRAAYELTRQKTRLDILVDGKPAQP
                     RDGAIRLAPSYGQLNAGITLTNKGDALVWRTVSVQGSPAQPLPAAANGLTLTKSVWTI
                     SGSVADLGNLRQNDRVIIELSGRMANNYAHRMGVIDLLPAGLGIEQALSGDEGKPYNL
                     NDPLTDTGMADKRDDRFVAAFTIGSSNRSHDPKRPEPQPVFHIAYVARAVTAGSFVMP
                     AGMAEDMYAPGTLARTTLGSVTIRP"
     gene            complement(5561..>6944)
                     /locus_tag="VGC27_03715"
     CDS             complement(5561..>6944)
                     /locus_tag="VGC27_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0281715.1"
                     /translation="IEAATPPEQGTLRSAWVQLGPGGGIEARAVVEGKACPAVYVDQH
                     ERAMQVRAAPDGKFATVCAALIPKEARQAAIMFRAYRAPPPHSGAGVAAWEAWIKEET
                     GLPLPGTDASEADWLAWSGAVNQKAQYHIVPLALPAPDPHRIVVLGDTGCRVKGKVLQ
                     DCSDPKAWPFAEIAAKAAKLKPDLVIHVGDYPSRESACPAGFAGCAGTPFGDNWPTWD
                     ADFFAPAKPLLAAAPWVFVRGNHEECERAGPGWLRLLGPLPFDAVAPCAAHLAPYSVP
                     LGAENLVVMDDANAPDTSIASNMIAVYRSEIAALANAPSPSWLLLHRPIWGAISGPLG
                     LPIGGNQTLIAAVGTSGIPAPVELMLSGHIHSFEALNYGPKDRVPPQLIAGIGGDRLE
                     PTPAILKGTVFQGKSGVFVKDGLSLPGHGFLLMVKSADGWTINVHDASGKTKRTCFFR
                     SGRIDCPKRD"
BASE COUNT         1153 a         2317 c         2278 g         1196 t
ORIGIN      
        1 cggatcgacg ccgaaatgcc cgtcgaagcg cttgtcttca taggctttca acatggcgag
       61 atagcgcggg ctgacctcgc tgacgccggt cttcatgcgc caatagccgc cgcgcgaaag
      121 aaaggcgcgc agcagcacgc catgatggtc caccacctcc ggcgagacgt cctgcacgtt
      181 ggtcatgtcg gggggaaagg caaaatccgc cgcgacgacg gcacagaaga atccggccgc
      241 cgcgaaggcg acggcggcga gacgatggcg gaacagcaaa cgcatcttct ggcgaatggc
      301 gagtagcgaa tggcgaatag aaataatctg cgttctccta ttcgctactc gctattccct
      361 actcgcccct tcacggcctg atcgtcacgc ttcccagcgt cgtccgcgcc agcgtgcccg
      421 gcgcgtacat gtcctccgcc atgccggccg gcatgacgaa gctgcccgcc gtcaccgcgc
      481 gcgccacata agcgatgtgg aacaccggct gcggttcggg ccgcttcgga tcgtggctgc
      541 ggttggacga gccgatggtg aacgcggcga cgaaacggtc gtcgcgcttg tccgccatgc
      601 cggtgtccgt cagcggatcg ttgagattgt acggcttgcc ctcgtcgccc gacagcgcct
      661 gctcgatccc cagccccgcg ggcaacagat cgatcacgcc catccggtgc gcataattgt
      721 tggccatgcg tcccgacagt tcgatgatca cgcggtcgtt ctggcgcaga ttgccgaggt
      781 cggccaccga gccgcttatc gtccacaccg atttggtcag cgtcaggccg ttggccgcgg
      841 cgggcaaagg ctgcgccggg ctgccttgga cggagaccgt gcgccacacc aaagcgtcgc
      901 ccttgttggt caaggtgatt cccgcgttca actgcccgta gctgggcgcg aggcggatgg
      961 caccgtcgcg cggctgggcc ggcttgccgt ccaccaggat gtcgaggcgc gtcttctggc
     1021 gggtcagctc gtaggccgcg cgcagcatcc acgccttctc ctgcgtcgtg gtccaattca
     1081 gccgcatgtc gagcttcatc tcctcgctct tggagagcaa ggccggaacg agttgcccct
     1141 gcccgttctc ggccgccagc gcgatggttc ccgcgatgtc gcgcagcagc gaaccgtaat
     1201 cgcccgccgg ataggtgttg ggcttggcct tggcgatgat ctcgcgggcg cggttgaagc
     1261 cgtaggcggc ccgcgaacgg tcgcccactg ccgccgccgc cgagccggtg agcgccgccg
     1321 cgatggcggt gttccattcc ggcccgcgcg tgtcgctgaa atagcgcaga tcgctgacgt
     1381 tcgcctgccc gctgcgcgcc aagagataga acgcataggc gcggctgttg tcgttgaagg
     1441 aacccgaggc cgaaatccgc ttcagccacg ccgcgccgcg ccgcagcgcg tcgttcggca
     1501 cgatgtagcc cttgtccttc gcctgcatca ggaaatcgag cgcgaaggcg ctgatccacg
     1561 gctcggcgtc ggagccgggt ccccacatgc cgaaatcgcc cgcgaagttc tgcatgtcga
     1621 gcacggaatc gacggcctgc tgctcgcgct cgcgcagcgc cttgtccttc ggcagcccgg
     1681 ccagatcggc gagatcgttg aagaacagca gcggcatggc gcggctagcg atctgttcga
     1741 cgcagccata gggatatttg tcgagccatt tgagcaggcc cggcacgttc gagtagccgt
     1801 gcgccgccga gacggtgagg cccaccgtct gcgttccggg aaccagatcg gcgatcaggg
     1861 ccttgttggc ggtgaagctt gtgcccgccg cgaacggcac ggtgtcgtcg cgggcgatgt
     1921 cgagctgcgg cgaacgcacc tcgatcggcc aggagcgtgt caccgagaag ccgtctgggc
     1981 ccgtcagctt gagcgcgata ttggcgatgc cgagcccggt gccgcgcacc tccaccggca
     2041 ccagcacgcg ctggccggcg ctgaggttct gcgtcaccac ggtctgagca ccgcccgcca
     2101 atcccactgg gccaccggcc gtgacggtcg cggtgtagct gcccggcctg ccttcgacat
     2161 tgttcaggtt gagggccgct ttggcgcggt cgcccggcgc caggaagcgc ggcagcacga
     2221 tctcggcgac caccggatcg cgcaccgtca gcggccggtc ggcatggccg agcttgcccg
     2281 cgctgaacac caccgccatc aggcgcagtt cgccgttgaa atcgggaacg tccagcggca
     2341 cggagaccat cccgccggcg ccgatcttca ccagccccga gaacaaggcc accgtcctgg
     2401 tcggcaccac cgccagcgcg cggcccccga acatgtcgcc gccctcgcgc atggcgccga
     2461 cggcgccctt ctcggccttg atcagacggc cgtaatcgtc gcgcatctcg acgccgagtc
     2521 tgcgcttgcc gtaataataa gagaccgggt cgggcgattt aaattcggtg agctgcaaga
     2581 tgccttcgtc cacggcggca agcgtgagat aggcctcctc gccccgcgac agccctttca
     2641 cctgcaccgg aatgttgagc ttctggcgcg gcaggatttt cttcggcccg ccgatcaggg
     2701 tggcgagcgt gcgcttcgaa ttgtcgagcg gcagccatac caagccgatg gaacgcaccg
     2761 gctcgcgccc gctcgcctgg tcgagcgggc ggtaatccgt caccagcaca taggcgcccg
     2821 cgccccaatc cgccgacacc ggaacgtcga tcgtcgctcc gcccgcgggc gcatcgacca
     2881 cctgcgaaga gaacaccttg tcgccggcca ccacgaccag cgccttgccg tccgcggcgg
     2941 gcttgatgcg cacatgcgcg gtttcgcccg cgcgatacgc ggatttgtcg gccgcgaccg
     3001 ggatgcggtc gggccggtcg ccggcgctcg acgccgccca gccggaatag tagcgggtgg
     3061 aactggaagc gcccgatttc ggatcggtga gggtgagccg gtaggtgccc catcccatcg
     3121 cctgcgccag cttgagggac gcgccggtgc cgatggacat ggtgctcgag gtgatcagcc
     3181 ggtcgcgggt gaccgacttg tatttccagc cgccattgtc ctggtaccat tggtacgacg
     3241 tatcctcgcg cacccaggag acggtcacgc cggagagcgc gatgcgcttg ccgccggcgt
     3301 cgagcgccac ggcttcaaag cccgcccgcg ccccttcggc gaccgacgcg ccgctgaatt
     3361 gcggccggat gccgataaag acatcgtggt tgcgcaccgg gatttccacc gtcttggcgg
     3421 tgacgcgccc gcccggctcg tggatggaga ccttcaccat cgctctcagc gccaaggtgg
     3481 tgtcggcgag atcgccgatc gtgccggcgg cttcggtcga accgttcgcg tcggtcggcg
     3541 gcaccgtcat ctgcacatcg atgtcggaga aactgtcgtc cacccggccg aactgccagc
     3601 cggcgtaatt gtcgaacggc gtgggatcga ccgaaatccg cgccacggcc tcgcccgaaa
     3661 gcccgctcgc cggcgcgcca tagaggaagc ggctttcggc gcgcactttg atctcgctgt
     3721 tctggcgggc gaccttggtt tccgcgctca gcgtcacttt cagcttctgc ggcacgaaat
     3781 cctgcacgtc gaactgcacc cggccgatgg ccgtggcgtc cttcgaggtg tcgatgaaag
     3841 ccgcgatctg ccagcgcccg tgcggcgcgc tggaagagag cggcagcgtc cacgtcgccg
     3901 cgccggcctt gagcgcggcg ccgggaagcg tgacgcggcc gacttcgagc ccgtcgggcc
     3961 gcgtcgcgac gagcgtgagc ggcgcggtcg ccgcattgcc gatgcggtcg cgcaacaagg
     4021 cggtgagata caccgtctcg ccggaacgat agaccccgcg ttcggtgtag agataggcat
     4081 cgaccggtcc gggcacgttg cgcccgctca cgccgcgatc ggtgagatcg aagctggaac
     4141 ggcgcagatc ggtgaagctg aaatcctgat cgttgccgta ggccatgacc acgaccggct
     4201 cgtcgccgcc tttggcgcgc atcaggccgg cgtcgaaatc ggcgcggccg tccgagttcg
     4261 ttttcgccgt ggcgacttcg ttgttgttgc gcgccaccag cgagagcttg acgccctcga
     4321 gcggcttggc gctggcgtag gagcgcgcga acacggtcaa tccgcccgcg ccggtgaagg
     4381 tggtgagcgc gatgtcggaa tcgaccaccc attgcaccgc catttcgccg gacgagaagc
     4441 cttcgtcgga atcgtcgccg cctttcttgc tggcgtccga ggcgatcagg acataggcgc
     4501 cgggcttgcg cttcttcagg actttatgga tcgggatcaa cgtgaccacg gaatcgttct
     4561 tgacgttggc gacgtccatc gtgccttgcc agaccagcga accctgattg ttctggatct
     4621 cgttcgcgct ccaggaatag agggtggttt cgtccaccgt gccgctttcg atctgcgaca
     4681 gcaggcggtc gccgacgcgg atcagcttca gcttgatctt ggcgacgttg acggtcgtga
     4741 ccggcacgcc ttgggcgttg tcgcgcggca ggatgatgcc gccgttgaag cgcacgatcg
     4801 agggcttgtc gcgcagctcg acgggaacgg tttcgctctc gaccagtttg tcgcccgacg
     4861 ccgagggcag cccggctttc aaggtgacgt tgtagatctt gttgaaatcg aggcccgaca
     4921 ggcacagccg ctgatcgacg acgcggacgg ccacgcgcgt tgccggatcg atcgagaaat
     4981 aatcctcgta acgggtgtgc cccgatggat cgagcgcacg ggtgaagacg atgcacgctt
     5041 cgggccggtc tttcgaggtg tcgatatcga cacggcggaa gacaaactcg ccgccggctt
     5101 cggccgcggg cggcgcgact gcggaggtta cggtagagag accgcccgcc aaccggtcga
     5161 agaacccgct gccgccggtt ccgcgtccgc ccaacacata gagcgtgacg accacggcga
     5221 gaaccacggc aacgccgacg ccgccgccga tgagaatcca atggcgatat tttcccatcg
     5281 cacgcccccc tttagcctgg ccggagatta caaccgcaaa acgtatgcgg caattcgctc
     5341 agcttcgccg gcaatcgtgg cggccgcctc accggcgcga agtatcctta cggcaaagcc
     5401 acttgctgcg gtgcagccag ccttcctaga aagtgatccc gtctagctga atcgctctgg
     5461 ccacccttcg acaagctcag ggtgagggga gtacctagtc cttcatgctg agcttgtcga
     5521 agcatgagta tggcgcgatc ccatgtgggt ggatcgcgct ctagtcgcgt ttcgggcaat
     5581 cgattcgtcc gctgcggaaa aagcaggtac gtttggtttt gccgctggcg tcatggacat
     5641 tgatcgtcca gccgtcggca ctcttgacca tcagcaggaa accgtggccg ggcagcgaca
     5701 gcccgtcctt gacgaagaca ccggatttgc cctggaacac cgttcctttc aggatcgccg
     5761 gcgtgggttc cagcctgtcg ccgccgatgc cggcgatgag ttgcggcggt acgcgatcct
     5821 tggggccata attcaacgct tcgaagctat ggatatgtcc ggacagcatc agctcgacag
     5881 gcgccgggat acccgacgtg ccgacggccg cgatcaaagt ctggttgccg ccgatgggca
     5941 ggccgagcgg ccccgatatc gcgccccaga tcgggcgatg aagcagcagc caactcggcg
     6001 acggcgcgtt ggccagcgcc gcgatttcgc tgcgatagac ggcaatcatg ttggaggcga
     6061 tgctggtgtc gggcgcattg gcgtcgtcca tcaccaccag gttctccgca cccagcggca
     6121 cgctataggg cgccagatgc gccgcgcagg gggcgacggc gtcgaacggc aacgggccaa
     6181 gcaacctcag ccagcccgga ccggcgcgct cgcactcctc atgattgccg cgcacgaaga
     6241 cccaaggcgc cgccgccagc agcggcttgg ccggggcgaa gaaatccgcg tcccaagtcg
     6301 gccaattgtc gccgaagggc gtaccggcgc agccggcgaa cccggccgga caggcgctct
     6361 cgcggctcgg ataatcgccg acatgaatga cgagatcggg tttgagcttg gccgccttgg
     6421 ccgcgatctc ggcgaacggc catgccttcg gatcgctgca atcctgcagt accttgcctt
     6481 tgacgcggca gccggtgtcg ccgagcacga cgatgcgatg ggggtcgggc gcgggaaggg
     6541 caagcggaac gatgtggtat tgcgcttttt ggttgacggc gccgctccag gccagccaat
     6601 cggcctcgct ggcgtccgtc cccggaagcg gcaggccggt ttcttccttg atccaagctt
     6661 cccatgcggc aacacccgcg ccgctgtgcg gcggcggcgc gcgataagca cggaacatga
     6721 tagcggcctg tctggcttcc ttcggaataa gcgccgcaca cacggtcgcg aatttcccgt
     6781 cgggcgcggc gcggacctgc atcgctcttt catgctgatc cacgtaaacc gcagggcagg
     6841 cttttccttc caccacggcg cgcgcttcga tgccgccgcc ggggccaagc tggacccaag
     6901 cggatcgcag cgtgccctgc tccggcggcg tcgccgcttc gatg
//
LOCUS       DASWPU010000164         4259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_44372,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000164 DASWPU010000000
VERSION     DASWPU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44372"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VGC27_03720"
     CDS             complement(<1..529)
                     /locus_tag="VGC27_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY0281716.1"
                     /translation="MSIIDAAQPKALTLSPELVEGSKGVPVSRVEDFFALLKPRVMSL
                     VVFTGLAGMIAAPGHVHPLTAFSVLLCIAVAAGASGALNMAYDSDIDALMARTASRPI
                     PQGRIAREGALAFGWTLAVGAVAMMLLFANALAAGLLAFTIFFYVGVYTLWLKRRTPQ
                     NIVIGGLAGALPPAIG"
     gene            complement(529..2106)
                     /gene="ctaD"
                     /locus_tag="VGC27_03725"
     CDS             complement(529..2106)
                     /gene="ctaD"
                     /locus_tag="VGC27_03725"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY0281717.1"
                     /translation="MAHADHPHGWRRYVYSTNHKDIGTMYLVFAVCAGVIGGLLSMAM
                     RLELAEPGLQIFANPHMFNVFVTGHGIIMIFFMIMPAMIGGFGNWLVPMMIGAPDMAF
                     PRMNNISFWLLPFSFMLLVASMFVEGDSGGTGVGGGWTLYAPLSSYGSPGPAMDFAIF
                     SLHIAGASSILGAINFITTIINMRAPGMTLHKMPLFVWSILVTAFLLLLSLPVLAGAI
                     TMLLTDRHFGTSFFNAAGGGDPVLYQHLFWFFGHPEVYILILPGFGMISHIVATFAKK
                     PVFGYLGMAYAMVAIGFIGFLVWAHHMYTVGLSVDTRAYFVFATMVIAVPTGIKVFSW
                     IATMWGGSIEFKTPMLFAIGFIFLFTVGGVTGVVLANAGVDVVLQDTYYVVAHFHYVL
                     SLGAVFAIFAGLYFWFPKFTGCMYNETWAKAHFWLTFVSVNLAFFPMHFLGLAGMPRR
                     IADYPDAYAGWNYVSSAGAFIAGAAALIFLATMIEAFVKKRRAGDNPWGVGATTLEWT
                     LSSPPPFHTFNELPKIS"
     gene            complement(2118..2939)
                     /gene="coxB"
                     /locus_tag="VGC27_03730"
     CDS             complement(2118..2939)
                     /gene="coxB"
                     /locus_tag="VGC27_03730"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY0281718.1"
                     /translation="MFLRKTAYAAALLAGFAGPAAAAPQNWQVGMQQAATPVMARIED
                     FHTLLLTIMVAVCVFVAALLVWVMLRYNQRVHPVPSKTHHNTMLEVAWTLLPVAILVV
                     IAIPSFRLLYFEAEIPKPDVTITAIGKQWFWTYEYPKDGAFQFDSYMLSDEAAAKAGK
                     PRLLGVDNPIYVPVNKVVEIDTTGADVIHSWAVPAFGVKMDAIPGRINRTWFKATKIG
                     TYYGACSELCGVNHAFMPIEVKVVSEAEYDAWLAVAKQKYAAIDNPGAVRVAADN"
     gene            3190..>4259
                     /locus_tag="VGC27_03735"
     CDS             3190..>4259
                     /locus_tag="VGC27_03735"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY0281719.1"
                     /translation="MAGDLFFSRTGMDRARVQAKVDEALKGADDGELFLEFCQSESFS
                     FDDGRLKAANFDTSQGFGLRSVCGESTGYAHANELSEEAIGRAAETVRAVARGHSGAA
                     SAAPARSNVKLYTDIDPLQSAGFEAKVKLLEEMNAYARNKDSRVHQVSCSLAGEWQNV
                     EIIRPGGETYRDIRPLVRLYVSVVVEENGRQESGSFGGGGRGGYETYLAADYWQAAID
                     EALRQALVNLSSVPAPAGEMTVVLGPGWPGILLHEAIGHGLEGDFNRKKTSAFAGLLG
                     QRVAAPGVTVVDDGTIGGRRGSLSIDDEGTPTSRTVLIEDGILKAYMQDRQNARLMGM
                     APTGNGRRQSYASPVMPRMTNT"
BASE COUNT          878 a         1351 c         1366 g          664 t
ORIGIN      
        1 agccgatggc cgggggcagc gcgcccgcca gcccgccgat cacgatgttc tgcggcgtgc
       61 gccgcttcag ccacagcgta tagacgccga catagaagaa gatcgtgaag gcgagcaaac
      121 cggccgccag cgcattggcg aacagcagca tcatggccac cgcgccgacc gccagcgtcc
      181 agccgaacgc cagcgcaccc tcgcgcgcga tgcggccttg cggaatggga cgcgacgcgg
      241 tgcgcgccat cagcgcgtcg atgtcgctgt cataggccat gttgagcgcg cccgaagcgc
      301 ccgccgccac cgcgatgcac aagagcacgc tgaacgccgt cagcggatgg acgtggcccg
      361 gcgccgcaat catgccggcc agcccggtga agacgacgag cgacatcacg cgcggtttga
      421 ggagcgcgaa gaaatcttcc accctggaga cgggaacacc cttcgaccct tcgacaagct
      481 caggactcag ggtgagggcc ttcggttgtg cagcgtctat tatcgacatc agcttatctt
      541 cggcagttcg ttgaaggtgt ggaacggcgg cggcgaggaa agcgtccatt ccagagtggt
      601 cgcgcccacg ccccagggat tgtcgcccgc cctgcgtttc ttcacgaagg cctcgatcat
      661 cgtcgccagg aagatgagcg cggccgctcc ggcgatgaag gcgccggcgg aggagacgta
      721 gttccagccc gcataggcgt cgggataatc ggcgatgcgg cgcggcatgc cggccagccc
      781 caggaagtgc atcgggaaaa aggccagatt gacgctcacg aaggtaagcc agaaatgcgc
      841 cttggcccag gtctcgttgt acatgcagcc ggtgaatttc gggaaccaga aatacagccc
      901 cgcgaagatc gcgaataccg cgcccagcga cagcacgtaa tggaaatgcg ccacgacgta
      961 ataggtgtcc tgcagcacga cgtcgacgcc ggcattggcc agcaccacgc cggtcacgcc
     1021 gccgaccgtg aacaggaaga tgaagccgat cgcgaacagc atcggcgtct tgaactcgat
     1081 ggagccgccc cacatggtcg cgatccagga gaacaccttg atgcccgtcg gcacggcgat
     1141 gaccatggtg gcgaagacga aatacgccct ggtgtccacc gaaaggccca ccgtgtacat
     1201 gtggtgcgcc cagaccagaa agccgatgaa gccgatcgcc accatcgcat aggccatgcc
     1261 gagatagccg aacaccggct tcttggcgaa ggtggcgacg atgtggctga tcatgccgaa
     1321 gcccggcagg atgaggatgt aaacctcggg atgaccgaag aaccagaaca ggtgctggta
     1381 aagcaccgga tcgccgccgc ccgccgcgtt gaagaagctg gtgccgaaat gacggtcggt
     1441 gagcagcatg gtgatggcgc ccgccagaac cggcagcgac agcagcaaga ggaacgccgt
     1501 caccaggatc gaccagacga acagcggcat cttgtgcagc gtcatgcccg gcgcgcgcat
     1561 gttgatgatg gtggtgatga agttgatggc gccgagaatc gaggaggcgc ccgcgatgtg
     1621 cagcgagaag atggcgaaat ccatcgccgg ccccggcgaa ccgtaggacg acagcggggc
     1681 atacagcgtc cagccgccgc ccacgccggt gccgccggaa tccccttcca cgaacatgga
     1741 agcgaccagc agcatgaacg agaacggcag cagccagaac gagatgttgt tcatgcgcgg
     1801 aaacgccatg tccggcgcgc cgatcatcat cggcaccagc cagttgccga agccgccgat
     1861 catcgccggc atgatcatga agaagatcat gatgatgccg tgaccggtca cgaacacgtt
     1921 gaacatgtgc ggattggcga aaatttgcag gcccggctcc gccagctcca aacgcatcgc
     1981 catcgacaag aggccgccga tcactccggc gcagaccgca aacaccaggt acatcgtgcc
     2041 gatgtccttg tggttcgtcg aatagacgta gcgccgccag ccgtggggat ggtctgcgtg
     2101 ggccatgttc gccgtcctta attatctgcc gcgacgcgga ccgcgccggg attgtcgatt
     2161 gccgcgtatt tctgcttcgc caccgcgagc caggcatcgt actcggcctc gctcaccacc
     2221 ttcacctcga tcggcatgaa ggcgtgattg acgccgcaca gctcggagca tgcgccgtaa
     2281 taggtgccga tcttcgtcgc cttgaaccag gtgcggttga tgcggccggg aatggcgtcc
     2341 atcttcaccc cgaaggccgg aaccgcccag gaatggatca cgtccgcgcc ggtggtgtcg
     2401 atttcaacca ccttgttcac cggcacatag atcggattgt ccacgcccaa gaggcgcggc
     2461 ttgccggctt tcgccgcggc ctcgtcgctc agcatgtagg aatcgaactg gaaggcgccg
     2521 tccttgggat attcgtaggt ccagaaccac tgcttgccga tggcggtgat ggtgacgtcg
     2581 ggcttgggaa tctccgcctc gaaatagagc agccggaacg acggaatcgc gatgaccacc
     2641 agaatggcga ccggcagcag cgtccaggcc acttccagca tcgtgttgtg gtgcgtcttg
     2701 ctgggcaccg ggtggacgcg ctggttgtaa cggagcatca cccagaccag cagcgccgcg
     2761 acgaagacgc agaccgccac catgatcgtg agcagcaggg tgtgaaaatc ctcgatccgc
     2821 gccatcacgg gggtggcggc ctgctgcatc ccgacctgcc aattctgcgg cgcggccgcc
     2881 gccgggccgg caaaacccgc caagagggcc gcggcgtagg cggtctttct gaggaacatg
     2941 gccacctcgt gagaattggg acgcgcgcgg tctttggcgc tccccccgga accggatttt
     3001 agaaaaattc tatattcgat tttgagacgt acttgacact actgtcattg cgactttatg
     3061 gcgcggacgg cctggacgca atttggcccg cattatgccc ttgcgcgggc gcaccagaac
     3121 accgatatgt gaaaagccgc cgtttgcgcc ggaaatcgcg ccggagacgg cagaaaacgg
     3181 aggcttctct tggcaggcga tctctttttc tcccgcaccg gcatggaccg cgcccgtgtc
     3241 caggccaagg tcgacgaggc cctgaagggt gccgacgacg gcgagctgtt cctggaattc
     3301 tgccagagcg aatcattttc cttcgacgac ggccggctga aggcggccaa tttcgatacc
     3361 agccagggct tcggcctgcg ctccgtgtgc ggagaatcga ccggctacgc ccatgccaac
     3421 gaactctccg aagaggccat cggacgcgcc gccgaaaccg tgcgcgccgt cgcgcgcggg
     3481 cattcgggcg cggcgtccgc cgcccctgcc cgcagcaatg tgaagctcta caccgacatc
     3541 gatccgcttc agagcgccgg tttcgaagcg aaagtgaagc tgctcgaaga gatgaacgcc
     3601 tacgcacgga acaaggattc gcgggtccat caagtatcgt gcagcctcgc gggcgaatgg
     3661 cagaacgtcg agatcatccg cccaggcggc gaaacctatc gcgacatccg cccgctggtg
     3721 cggctctatg tgtcggtggt ggtggaagag aacggccgcc aggaatccgg cagcttcggc
     3781 ggcggcggac gcggcggcta cgagacctat ctcgccgccg attactggca ggcggccatc
     3841 gacgaagcct tgcggcaggc gctggtcaat ctctcgtcgg tgcccgcgcc ggccggagag
     3901 atgacggtgg tgctcggccc cggctggccg ggaatcctgc tgcacgaagc catcggccac
     3961 gggctggaag gcgacttcaa ccgtaaaaag accagcgcct tcgcgggcct gctcggccag
     4021 cgcgtggcgg cgcccggcgt cacagtggtc gacgacggca ccatcggcgg acggcgcggc
     4081 tcgctctcca tcgacgacga aggcacgccg acctcgcgca cggtgctgat cgaagacgga
     4141 atcctgaaag cctatatgca ggaccgccag aacgcgcggc tgatgggcat ggcgcctacc
     4201 ggcaacgggc ggcggcaatc ctacgccagc ccggtcatgc cgcgcatgac caacaccta
//
LOCUS       DASWPU010000165         5041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_38281,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000165 DASWPU010000000
VERSION     DASWPU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38281"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGC27_03740"
     CDS             complement(<1..192)
                     /locus_tag="VGC27_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEY0281720.1"
                     /translation="MSVLWTSAEVESATLGKASAPFAATGLSIDTRTLKPGDLYIAIK
                     GEKRDGHDFVREAFEAKAAA"
     gene            complement(189..1682)
                     /locus_tag="VGC27_03745"
     CDS             complement(189..1682)
                     /locus_tag="VGC27_03745"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY0281721.1"
                     /translation="MMGPHSGVNIAAGKAGIGPMESVKQFAGLASDSREVGPGYLFAA
                     LPGTKANGAEFVKDAVSRGAVAVLGRPEVAPRAAALGVRFIADDNPRRALARIAAQFF
                     AAQPETVAAVTGTNGKTSVTVFLREIWTALGRRAASMGTIGVVTPEGKIKLHHTTPDP
                     IELHRLLAELKQGGIGHLAVEASSHGLDQYRLDGVKIAAVGFTNISRDHMDYHPSFEA
                     YLACKLRLFTELAQGAAAAVVNADTEHAGSFIAAAKTRGLRLLTVGEHGESLKLLART
                     PHENGQSLKIAYDGNTYTVALPLAGAFQVSNALVAAGFAIGLGEKADAVFAALAGLEG
                     APGRLEKVAYAKSGASIYVDYAHTPDALETILTALRPHTKGRLHVVFGCGGDRDKGKR
                     PLMGAAAAKYADRVIITDDNPRGEDASAIRKQALALCPGAQEIGDRAEATRAGIAALG
                     EGDVLVIAGKGHESGQIVGTVIHPFSDRDEAIKAATALGGRPVERRT"
     gene            complement(1679..3277)
                     /locus_tag="VGC27_03750"
     CDS             complement(1679..3277)
                     /locus_tag="VGC27_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY0281722.1"
                     /translation="MNAVPTVFQRRIVIGAVMCAAAFALVGVRLIDVTLFIHDTRAAK
                     RGIAGEIARGDIVDRNGELVARDLPAYDLYARPHMFADRRLAARRLAAATGASERRLT
                     QMFTGNRAYVLVARQISPQRRAEVGRLDLPGLEYEPNSKRYYPDGRMTAQVIGVTDPD
                     GNGVSGLELGLDPRLRGERPNEAVQTSLDTRVQFILAHEAAVSMKEFSARAAGGLVMD
                     VRSGEILALVSLPDFDPNVRAFAAGTSPRNIMAQDVYELGSVFKIFSFALALEERTIT
                     DLDERIPIGSGFKIGKYTIHEAERLPATLAARDVLAQSSNIGTAQIALRSGPSRQRAF
                     LTRMGLLSAVRSELPESARPLYPSNWGQVETATIGFGHGVSVSPLAFAAATAAVVNGG
                     RRIVPTFLKHPADARGEQLISPATSATMRKLLRYVVTGGTGKKADAPGYDVGGKTGSA
                     EKVGPHGKYTRKLVTSFCGIFPMEDPRYLVFVVLDEPHGTKATMGFALAGYTAAPLVG
                     RVIARIAPLLGVPMKPVAVAKENS"
     gene            complement(3285..3662)
                     /locus_tag="VGC27_03755"
     CDS             complement(3285..3662)
                     /locus_tag="VGC27_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281723.1"
                     /translation="MIRVFNFFCVAAMGLTILTLYRVSEQTRVAQVQLSRIEHRIAGE
                     RTKMSVLQAEWVRVASPERIQQLAESSLGMSDTATVQLSSLELLPRRGDAGAPLALMR
                     EASAQAPLAAPAELVKVAAHTGM"
     gene            complement(3672..4592)
                     /gene="rsmH"
                     /locus_tag="VGC27_03760"
     CDS             complement(3672..4592)
                     /gene="rsmH"
                     /locus_tag="VGC27_03760"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY0281724.1"
                     /translation="MGHLPVMLAEVLEALEPRDGAHYIDGTFGGGGYARAILEACDCR
                     VLGIDRDPEAIKRGEKLVALYPGRLTLVHGEFSQMDRLLAGTGESGTNGVVLDLGVSS
                     FQFDEAARGFSFRADGPLDMRMSLSGLSAADVVNTEDEQTLAGIIARYGEERRARRIA
                     RAIVQARPLTRTVELAELVARALGPAAARQPIHPATRTFQALRIHVNDELGELSRALE
                     AATRALKPQGRLAVVAFHSLEDRIVKQFLIARSGAAPRASRHAPAERRASPPLYRLLA
                     ARPQTPSAAEIANNPRARSAKLRTAERLAA"
     gene            complement(4592..>5041)
                     /locus_tag="VGC27_03765"
     CDS             complement(4592..>5041)
                     /locus_tag="VGC27_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281725.1"
                     /translation="VQPFISTVTGTLDTKGRVCVPASFRQILAAQNTQGVYFCPSFSE
                     ATLEGFGEEVLQAFHRQIAEVNPFFTKDFNAKAAVVLGETKLLPLDENGRVRLPEELI
                     AHAGLKDGVVFVGMGTKFQIWEPARFAEVRAARLKEALAAFNAEGGA"
BASE COUNT          840 a         1668 c         1698 g          835 t
ORIGIN      
        1 ggccgcggcc ttggcttcga acgcttcgcg aacgaaatca tggccgtcgc gcttctcgcc
       61 cttgatcgcg atatagaggt cgcccggctt gagtgtgcgc gtatcgatgg aaagcccggt
      121 tgccgcgaac ggcgcggacg ccttgcccaa ggtcgcgctt tcgacttccg ccgaggtcca
      181 caagacgctc atgtccggcg ctccacgggg cggccgccca acgcggtcgc ggccttgatc
      241 gcttcgtcgc ggtcggaaaa cggatgaatg acggtgccga cgatctggcc gctttcgtga
      301 cccttgcctg cgatcaccag aacgtcgcct tcgccgagcg ctgcaatacc ggcgcgggtc
      361 gcctcggcgc ggtcgccgat ctcttgtgca ccgggacaga gggccagcgc ttgcttgcgg
      421 atcgcgctag catcctcgcc gcgcggattg tcgtcggtga tgatgacgcg gtcggcatat
      481 ttcgccgccg ccgcgcccat cagcggacgc ttgcctttgt cgcggtcgcc gccgcagccg
      541 aacaccacat gcaaccggcc tttggtgtgc gggcgcagcg cggtcaggat ggtttcgagc
      601 gcgtcggggg tgtgggcgta atcgacatag atcgacgcgc cgctttttgc ataagcgact
      661 ttctccaagc ggccgggcgc gccctcaaga ccggcgagcg ccgcgaacac cgcgtccgct
      721 ttttcgccca gcccgatggc gaaaccagcc gcgaccagcg cgttggatac ctggaaggca
      781 ccggccaaag gcagcgcgac ggtataggtg tttccgtcat aagcgatctt cagcgattgg
      841 ccgttttcat gaggggtgcg cgcgagcagc ttcaggctct cgccatgctc gccgaccgtc
      901 agaagccgaa gtccgcgcgt cttggcggca gcgatgaaag atcccgcatg ctccgtatcg
      961 gcattgacca ccgccgcggc ggcgccctgc gccagttccg tgaacagccg cagcttgcag
     1021 gcgagatagg cttcaaagct ggggtgataa tccatatggt cgcggctaat attggtgaag
     1081 ccgacggccg cgatcttgac gccgtccagg cggtattgat cgaggccgtg actggatgct
     1141 tccacggcaa gatggccgat accgccctgc ttaagttccg ccagaaggcg atgcagttcg
     1201 atcgggtccg gcgtggtgtg gtggagcttg atcttgccct ccggcgttac cacgccgatg
     1261 gtgcccatgc tggccgcgcg cctacccagc gccgtccaga tttcgcgcag gaaaaccgtg
     1321 accgaggtct tgccgttggt gccggtcaca gcggcgacgg tctcgggctg ggcggcgaag
     1381 aactgcgcgg cgatgcgcgc cagcgcccgg cgcggattgt cgtcggcgat gaagcggacg
     1441 ccgagcgcgg ccgctctagg ggcgacctcg ggccggccga gcacggcgac ggcgccgcgg
     1501 ctcaccgcgt ccttcacgaa ttcggccccg ttcgccttgg ttcccggcag cgccgcgaac
     1561 agatagcccg gccccacctc gcggctgtcg ctcgcaagcc cggcgaattg tttcaccgat
     1621 tccatagggc ctatgcccgc cttacccgcc gcgatgttca cacccgaatg tggtcccatc
     1681 atgaattctc tttggcaacc gcgacgggct tcatcggcac gcccagcaac ggcgctatcc
     1741 gtgcgatcac acgacccacc agcggcgccg cagtatagcc ggccagcgca aaacccatcg
     1801 tcgccttggt gccgtgcggc tcgtccagca cgacgaagac cagatagcgc ggatcttcca
     1861 tgggaaagat gccgcagaac gaggtgacca gcttgcgggt gtatttacca tgcggaccga
     1921 ccttttccgc cgatccggtc ttgccgccga catcgtagcc gggggcatcc gccttcttgc
     1981 cggtgccgcc ggtgaccaca tagcgcagaa gcttgcgcat ggtcgcgctg gtggcgggcg
     2041 aaataagctg ctcgccgcgg gcgtcggcgg ggtgcttgag gaaggtgggc acgatccgcc
     2101 gcccgccatt gacgaccgcc gccgtcgccg ccgcaaacgc caacgggctg accgagaccc
     2161 cgtgaccgaa accgatggtg gccgtctcca cctggcccca attcgaggga tagagcgggc
     2221 gggcggactc cggaagctcg cttctgaccg ccgacagcag ccccatcctg gtcaagaagg
     2281 cgcgctggcg gctggggccg gagcgcaacg cgatctgcgc cgtgccgatg ttggaggatt
     2341 gcgccagcac gtcgcgcgcg gcgagcgtgg cgggcaaacg ctcggcttcg tggatcgtgt
     2401 acttgccgat cttgaagccg ctaccgatcg gaatccgttc gtcgaggtcc gtaatcgtgc
     2461 gctcctcaag cgccagcgca aacgagaaga tcttgaagac cgagcccaac tcgtaaacgt
     2521 cctgcgccat gatgttgcgc ggagatgtcc cggcggcgaa ggcgcggaca ttgggatcga
     2581 aatccggcag cgacaccagc gcgagaatct cgccgctgcg cacgtccatc accagcccgc
     2641 cggcggcccg cgcgctgaat tccttcatgc tgaccgcggc ttcatgggcc aggatgaact
     2701 gcacgcgcgt atcgagggag gtttgcacgg cttcgttcgg ccgttcgccg cgcagcctgg
     2761 gatcgaggcc aagctccaga cccgagacgc cgttgccgtc gggatcggtg acgccgatga
     2821 cctgcgccgt catgcggccg tcgggataat agcgcttgct gttcggctca tattccaggc
     2881 cgggcagatc caagcgtccc acctcggcgc gccgctgcgg cgaaatctgg cgggcgacca
     2941 gcacataggc gcggttgccg gtgaacatct gtgtcaggcg ccgttcgctg gcgccggtcg
     3001 cggctgccag ccggcgcgcg gcgaggcgac ggtccgcaaa catatgcgga cgggcgtaga
     3061 gatcgtaggc cggcagatcg cgcgccacca gctcgccgtt gcggtcgaca atgtcgccgc
     3121 gcgcgatttc gccggcgatc ccgcgcttgg ccgccctggt gtcgtggatg aacagagtca
     3181 cgtcgatcag ccgcacgccc accagtgcga acgccgcagc gcacatcacc gccccgatga
     3241 cgatacgtcg ttggaagacg gtgggcaccg cgttcatatg aatcctacat tccggtgtgg
     3301 gcggcgacct tgaccagttc cgccggagcc gccagcggcg cttgcgcgct ggcctcgcgc
     3361 atcagggcaa gcggcgcgcc ggcgtcgccg cggcgcggca agagttcgag cgaggaaagc
     3421 tggaccgtcg ccgtgtcgct catgcccagc gacgattcgg caagctgctg gatgcgctcg
     3481 ggactcgcca cgcggaccca ctccgcctgc agcacgctca tcttcgtgcg ctcgccggcg
     3541 atgcgatgct cgatgcggct gagctggacc tgcgctacgc gggtctgttc ggagacccga
     3601 tagagcgtca gaatcgtcag gcccatcgcc gccacacaga agaagttgaa gactcggatc
     3661 atcgtgttct cctaagccgc cagacgttcg gccgtccgca atttggcgga gcgggcacgg
     3721 ggattgttcg cgatctcggc ggcgctaggc gtctgcggac gcgcggcgag aagccggtaa
     3781 aggggtggag aggcgcggcg ctccgccggc gcatggcgcg aagcgcgcgg cgccgcgccg
     3841 ctgcgcgcga tgaggaactg tttgacgata cggtcttcga gcgaatggaa cgcgaccacg
     3901 gcaaggcggc cttgcggttt caacgcgcgc gtcgcggcct cgagggcgcg cgacagctcg
     3961 cccagctcgt cgttcacatg gatgcgcagg gcctggaagg tgcgcgtcgc gggatggatg
     4021 ggctggcgcg cggcggcggg accgagcgcg cgggcgacaa gctcggccag ttcgacggtg
     4081 cgcgtcagcg gccgcgcctg gacgatggcg cgggcgatgc gccgggcccg gcgctcctcg
     4141 ccgtagcgcg cgatgatgcc ggccagcgtt tgttcgtctt cggtgttcac gacatctgcg
     4201 gccgaaagac cgctcaggct catgcgcatg tcgagcgggc cgtcggcgcg gaaggaaaat
     4261 ccccgcgcgg cctcgtcgaa ttgaaacgac gatacgccaa ggtccaagac cacgccgttg
     4321 gtgccgcttt cgccggtgcc ggccagcagg cggtccatct gcgaaaactc gccatgcacc
     4381 agcgtcagac ggccgggata aagcgccacc agcttctcac cgcgcttaat cgcctcggga
     4441 tcgcggtcga tgccgagcac gcggcaatcg caggcttcga ggatggcgcg ggcatagccg
     4501 ccgccgccga acgtcccgtc gatgtaatgg gcgccgtcgc gcggctccag cgcctccagc
     4561 acttcggcga gcatcacggg aaggtggccc atcaagcgcc tccctcggcg ttgaaggccg
     4621 caagcgcttc tttgagccgc gccgcgcgga cttccgcgaa acgcgccggt tcccagatct
     4681 ggaatttcgt gcccatgccg acgaagacga cgccgtcctt caggccggca tgcgcaatca
     4741 gttcctcggg caggcgaaca cggccgttct cgtcgagcgg cagcagcttg gtctcgccaa
     4801 ggacaaccgc cgccttggcg ttgaagtcct tggtgaagaa cggattgacc tcggcgattt
     4861 gacgatggaa cgcctgcaac acctcctcgc cgaaaccttc cagggtcgct tcggagaagg
     4921 acgggcagaa atagacgcct tgcgtgttct gggcggcgag gatctggcga aaagacgcag
     4981 gaacgcagac ccgccccttg gtatcgagcg tgcccgtgac cgtcgaaatg aacggttgga
     5041 c
//
LOCUS       DASWPU010000166         7280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_62567,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000166 DASWPU010000000
VERSION     DASWPU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62567"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2040
                     /locus_tag="VGC27_03770"
     CDS             <1..2040
                     /locus_tag="VGC27_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY0281726.1"
                     /translation="GAAGMQELSAKGTASDLFGAASLAGVVTATGLNAQGVTGNAVVR
                     VDGKPDKLGLRLSSDLKDGDDGKANITAAAQLDLPGQKIVLTAFNGDWRGAALTLAAP
                     ATIDFSRGLAVDRLSAQIGGGDVVFAGRVLPVLAFNASLRNVALDAFKSFLPQLSPQG
                     TVSATVALTGAPGAPQGTITVQGRDLRARGISSRQMPSAAIDARAELLQGQATLTASL
                     TAGNNSRLTITGNAPLAMEKAMDLHLAGSLDLAMLDPFLAAAGRRVRGTATFDATAGG
                     SVNAPRLSGSAKLSDGEFQDYLQGIRIQAISAEAEAQGTAIRVTRFSGRAGHGSIEGS
                     GTIDLAAADTPVNFAIQAKNARPVVSDLITATLSGDAEVSGSLKGLLRLSGHLQVIRA
                     DINLPENFPPDIAVLDLRRRGQKPRPPPSRSQMTLDVAIRTTGPIFVRGHGVDAELGG
                     GIKLSGTSAAPVISGDFRMIRGTYSIAGQTLDFTSGKIGFDGTGVRNRLDPTLDFVAQ
                     TVSGGVTATLMVTGYASAPKIALSSTPPLPQDEVLSHLLFQQSVKQLTPLQLASMGQS
                     LVALGGVGSGFNPLGVLRKTFGLDRLAVGSAAAGASGSESQTTVEAGRYVMRNVYIGV
                     KQNLSGGTQTQVQVDIIRGLKAQATVSTSASATIAKGSAQEDNGSNIGLSYQFEY"
     gene            complement(2050..2123)
                     /locus_tag="VGC27_03775"
     tRNA            complement(2050..2123)
                     /locus_tag="VGC27_03775"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2089..2091),aa:Gln,seq:ctg)
     gene            2224..3225
                     /locus_tag="VGC27_03780"
     CDS             2224..3225
                     /locus_tag="VGC27_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEY0281727.1"
                     /translation="MEKGGQARKRRRRHGFSGPSGLPLLAAIDLGTNNCRLLIAAPEG
                     EGDFKIVDSFSRIVRLGEGVAQSGVLGEAAMERTVAALKICAQHIRRHGVRHVRAVAT
                     EACRQAANVQVLIDRVARETGLHLVVVSAEEEARLAAIGCGPLIGEDYDGALVFDIGG
                     GSTETIWLRKAGEVSLVHFASIPLGVMSLREGNGGGSFSAMREMMLARFSAVRREMDK
                     KAPFDVARHHLLGTSGTVTTLAGIALGLPRYVRARVDGTWHDCAEILSVVEMLKALDR
                     EGRAKLGCVGAERADLIVPGCAIFSAIQTVWPCARLRVADRGLREGILRELLKETRR"
     gene            3222..3911
                     /locus_tag="VGC27_03785"
     CDS             3222..3911
                     /locus_tag="VGC27_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY0281728.1"
                     /translation="MKEGDFGSGRGERRVRVTGARGRTVSSKRWLERQLNDPYVAAAK
                     AKGYRSRSAFKLKELDAKFHLLKRGARVLDLGAAPGGWCQVAAERIGETGKIVAADIL
                     EMEPIAGVEFLQCDLLDAETPAALKRMLGGEADLVLSDMASPTTGHRETDHIRTLALF
                     EAALDLAEDVLRPGGALVGKVFQGGATGELLTRVKTRFAEVKHVKPPASRRESVELYL
                     VARGFKGAPRG"
     gene            complement(3936..4373)
                     /locus_tag="VGC27_03790"
     CDS             complement(3936..4373)
                     /locus_tag="VGC27_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281729.1"
                     /translation="MASFAADTCYRPREIMRTNAMKMRSRLFSGAAGLAIAVALSFGA
                     QAQTYPAHGQDQTSPEMTGNMPSGEQPMWVKHKARHKHHVRGVEQDTMSGQTPATAPS
                     VARSAQRHHRGVYAGQSGPQESTDAEAAATDRLNRQQLNAAWN"
     gene            4551..4847
                     /locus_tag="VGC27_03795"
     CDS             4551..4847
                     /locus_tag="VGC27_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281730.1"
                     /translation="MTSKRSPLIWADKIALFMALLVAAGALLCWAIGIVGLDGRSHSR
                     FDSAVFNWTVDVEAMLVLPLWLFLRIVDFIARALGRWLQIGSHRDSSGGLRLTS"
     gene            5124..6419
                     /locus_tag="VGC27_03800"
     CDS             5124..6419
                     /locus_tag="VGC27_03800"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY0281731.1"
                     /translation="MNTVTTTTSHSLTDQTVIGIRDVLDGRKRGVRSVLLFAGPAVIS
                     SIAYVDPGNFATNIQAGAGFGYTLLWVVLSASLLGMLFQALSAKLGIVTGRNLAEMCR
                     DHFPRRMVYGMWGVSELAAMSTDLAEFLGGALAIALLFDLPLMVGMVITGIATYGMLL
                     LEKKGFRPMEIVIGVPALVIGVCYLTELLIASVAWGEVARGAVLPSIPNAAALTITVG
                     IIGATVMPHALYLHSGLTQNRAPARTEGERKKLLRFSNREVVVALSFAGLVNMAMVIM
                     AAAAFHAGHSEVAEIQTAYHTLTPLLGGGAAVIFLVSLLVSGLSSSTVGTMAGQIIMQ
                     GFVGFRIPVWVRRLVTMLPAFAIVAIGVDPTRALVMSQVVLSFALPLPMLALVWFTRS
                     PAFMGGFANGRLTDAVAIAGTAAVLIFNGVLLAQAFGIA"
     gene            complement(6824..7159)
                     /locus_tag="VGC27_03805"
     CDS             complement(6824..7159)
                     /locus_tag="VGC27_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281732.1"
                     /translation="MQHMHMLPHIIMMGIPIFIMAFICSQHCFIISAVMPPIGVILQT
                     MELPDISQLMSHAIIGIIIGIICGIIGMPPMFIIGIWGIMFMPPMFIIGIAGIITISW
                     LRPDAVPLG"
BASE COUNT         1222 a         2238 c         2431 g         1389 t
ORIGIN      
        1 ggtgcggcgg gcatgcagga gctttccgcc aaagggaccg ccagcgatct gttcggcgca
       61 gcgtcgcttg ccggcgtggt caccgcgacg gggctgaacg cccaaggcgt cacgggcaat
      121 gcggtcgtgc gtgtcgacgg gaagccggac aagctcggtc tccgtctttc ctccgatctc
      181 aaggatggcg atgacggcaa ggccaatatc accgcggcgg cgcagctcga cttgcccggg
      241 caaaaaattg tgctcaccgc gttcaatggc gattggcgcg gtgcggcgct gacgctggcg
      301 gcgccggcca ccatagattt ttcgcgcggc cttgcggtgg accggttgtc cgcgcagatc
      361 ggcggcggtg acgtcgtttt cgccgggcgc gtgttgccgg tgctcgcatt caatgcctcg
      421 ctccgtaacg tcgcattgga tgcgttcaaa tcctttctgc cgcagctttc gccccagggc
      481 accgtgtccg ccacggtggc gctgaccggt gcgccgggcg cgccacaggg cacgatcacg
      541 gttcaagggc gcgatctgcg ggcgcggggc atttcgagcc ggcaaatgcc gtccgccgcg
      601 atcgatgcgc gcgccgagtt actccagggg caggcgacgc tgacggcaag tttgacggcg
      661 ggcaacaaca gccgtctcac gatcacgggc aacgcgccgc tggcgatgga aaaagcgatg
      721 gatctgcatt tggccggcag cctcgatctt gcgatgctcg atccgtttct ggcggcggcc
      781 ggccggcggg tgcggggcac ggcaacgttc gatgcgacgg ccggcggctc cgtcaacgcg
      841 ccgcgcctca gcggcagtgc gaagctgagc gacggcgaat ttcaggatta tctgcagggc
      901 atacggattc aagcgatatc cgccgaagcg gaggcacaag gcacggcgat ccgcgtcacc
      961 cggttttccg gccgtgccgg tcacggctcg atcgaaggca gcggcacgat cgatctggcg
     1021 gcggcggaca cgcccgtcaa ttttgcgatc caggcgaaga acgcacggcc ggtcgtcagc
     1081 gatctcatca ccgccacctt gtccggcgat gccgaagtgt cgggttcgct gaagggtctg
     1141 ctccgtctgt cgggccatct tcaggtgata cgggccgaca tcaatctgcc ggagaatttt
     1201 ccgccggaca ttgccgtgct cgatctgcgc cggcgcggac agaaaccccg gccgccgccg
     1261 tcccggagcc agatgaccct ggatgtcgcg atcaggacca ctggtccgat tttcgtgcgc
     1321 ggtcacggcg tcgacgcgga attggggggc gggataaaac tttccggcac aagcgccgca
     1381 ccggtgatca gcggcgattt ccgtatgatc cgcggcacct acagtattgc gggccagacg
     1441 ctggatttca cctccggcaa gatcggattt gacggtaccg gggtgcgcaa tcgcctggat
     1501 ccgacattgg atttcgtggc gcagacggtg tcgggcggcg tgacggccac gctgatggtg
     1561 acgggttatg cctcggcccc gaagatcgcc ctgtccagta cgccgccact tccgcaagac
     1621 gaggttctgt cgcatcttct gttccagcag agtgtcaagc agttgacgcc gctgcagctg
     1681 gcgagcatgg gccagtcgct tgtcgcgttg ggcggcgtcg gcagcggctt caatccgctg
     1741 ggcgtgctgc gcaaaacgtt tggtctggat cgccttgcgg tcggcagtgc ggcagccggc
     1801 gcctcgggaa gcgaaagcca gacgacggtc gaggcgggcc gctatgtgat gcgaaacgtc
     1861 tatatcgggg tgaagcaaaa tctttccggc ggtacgcaga cacaggtgca agtggacatc
     1921 atccgcggcc tcaaggcgca ggccaccgtc agcacgagcg cgagcgcgac aatcgccaaa
     1981 ggcagtgcgc aggaagacaa tggcagcaat atcggcctca gctaccagtt cgagtattga
     2041 gctgtaggct ggctggggga ctaggattcg aacctagata ggcagagtca gagtctgcag
     2101 tcctaccgtt agacgatccc ccaaggggcg ctccgtttag cccaggttcg cggccctttt
     2161 caaggagata cgcacggccc ccgcccgtgc tagcgtcgcg gccgcaaatc accggggtcg
     2221 gcgttggaaa agggcgggca agcgcgcaaa aggcggaggc ggcacggttt cagcggtccc
     2281 tccggtctgc cgctgctggc cgccatcgac ctgggcacca acaattgccg cctgctgatc
     2341 gccgcgcctg aaggcgaggg cgatttcaag atcgtggatt ctttctcgcg catcgtccgg
     2401 ctgggggagg gggtggcgca gagcggcgtg ctcggcgagg ccgcgatgga gcgcaccgtc
     2461 gcggcgctga agatctgcgc ccagcacatt cgccgtcacg gcgtgcgcca tgtccgtgcc
     2521 gtcgccaccg aggcgtgccg gcaggccgcc aatgtgcagg ttctgatcga ccgcgttgcg
     2581 cgggagactg gccttcacct ggttgtggtc agcgctgaag aggaggcgcg cttagcggcg
     2641 atcggctgcg gcccgctgat cggcgaggac tatgacggcg cgctcgtctt cgacatcggc
     2701 ggcggctcga cggaaacgat ctggctacgc aaagccggcg aggtctcgct ggtgcatttc
     2761 gcatcgattc cgcttggcgt catgtcgctg cgggagggca acggaggtgg ttcgttctcc
     2821 gccatgcgcg agatgatgct tgcccgcttc agcgcggtgc ggcgcgagat ggacaagaag
     2881 gcccccttcg acgtggcgcg ccatcatctg ctcggcacct ccggcacggt gacgacgctg
     2941 gcgggcatcg ctctggggct gccgcgctat gtccgtgccc gcgtggacgg aacgtggcat
     3001 gattgcgccg agattctttc ggtggtggag atgctgaaag cgctcgaccg ggagggccgc
     3061 gccaagctgg gctgcgtcgg cgcggaacgg gccgatctga tcgttcccgg ctgcgccatc
     3121 ttcagcgcca tccaaacggt gtggccctgc gccaggctgc gcgtcgccga ccgcggcctg
     3181 cgggagggca ttctgcgcga actcctgaag gagacacgcc gatgaaagag ggtgatttcg
     3241 gctccgggcg cggcgagcgg cgggtgcggg tgaccggtgc gcgcgggcgc accgtctcgt
     3301 ccaagcgctg gctggagcgc cagctcaacg atccctacgt cgccgcggcc aaggcgaagg
     3361 gctatcgctc gcgctcggcc ttcaagctca aggagctgga cgccaagttt catctgttga
     3421 agcgcggcgc gcgggtgctc gatctcggcg ccgcgcccgg cggctggtgt caggtggcgg
     3481 ccgagcgcat cggcgagacc ggcaagatcg tcgccgccga cattctggag atggagccga
     3541 tcgccggagt cgaattcctg caatgcgatc tgctcgacgc cgaaaccccc gccgcgctca
     3601 agcgcatgct gggcggcgaa gcggacctgg tgctctccga catggcctcg cccaccaccg
     3661 ggcaccgcga gaccgaccat atccgcaccc tggcgctgtt cgaagcggcg ctcgatctcg
     3721 ccgaggacgt gctgaggccg ggcggcgccc tggtcggtaa agtgtttcaa ggcggcgcca
     3781 ccggcgagtt gctgacgcgc gtgaagacgc gcttcgccga ggtgaagcac gtcaagccgc
     3841 cggcctcgcg ccgggaatcg gtggagcttt acctcgtggc gcggggcttc aagggcgcgc
     3901 ctcgcggttg acccgcggca aggcggccgg atgctctaat tccacgcggc gttaagctgc
     3961 tgccggttga ggcgatccgt tgcggctgct tccgcgtccg tcgattcctg cggtccactt
     4021 tggcccgcgt agacgccgcg gtgatggcgt tgcgccgacc gtgcgaccga tggcgcggtg
     4081 gccggcgttt gcccgctcat cgtgtcttgt tctacgccgc ggacgtggtg cttgtgacgc
     4141 gccttgtgct tcacccacat gggctgctcg ccagacggca tgttgcctgt catctccgga
     4201 gaagtctgat cttggccatg cgccggatag gtttgcgcct gcgcgccgaa cgacaatgct
     4261 acggcaatcg ccagccccgc ggcgccggaa aacagacggg atctcatctt catggcgttt
     4321 gtcctcatga tttcgcgcgg tcggtagcac gtatcggcgg caaacgaagc catcgggaat
     4381 tacctacgcg atcgaggatg ccgaaccccg aacgcgcggc cgcccgccga cttgctcgcg
     4441 cgaacagtcc cgaaatgaca cggcctgagt aatttccgag tctttctcga cgggagcgca
     4501 tctcgatacc gtcttgggca ggcgacagga agcactcctg ggaaggcgcc atgacatcca
     4561 agcgaagtcc gttgatctgg gccgataaga ttgctctgtt catggcgctg ttggtcgccg
     4621 ccggagcgtt gctctgttgg gctattggaa tcgtcggcct ggatggccgc tcgcattcaa
     4681 ggttcgacag cgcggtgttc aactggacgg tcgatgtgga ggcgatgctg gttctgccgc
     4741 tttggctgtt cctgcggata gtggacttca tcgcgcgggc actgggccgg tggctacaga
     4801 taggttccca ccgcgacagt tccggcggat tgcggcttac ttcttaagcg cgcgagagct
     4861 gagattgagc tgcgggtcca cccggccggc gccttcgccg cgcacgaaat tctcttttta
     4921 aatatggctt ttcttcacga gcgccgggtc gcgcgcctga cgcttccgca gcgttttggc
     4981 cttcctgggt tcttcaggat ggaaaacaca tgatatttca atgggatatt ttgcttgcaa
     5041 ggggcttggg ttagtgcttt aatgcaatta agtcgcaaat gcatatagtt tgattagacc
     5101 ctatccgcgt agccggcccg accatgaaca ccgtcaccac cacgacctct cactcgctga
     5161 ccgaccagac cgtcatcggc atccgcgatg ttctggacgg acgcaagcgc ggggttcgct
     5221 ccgtcctgtt gtttgccggg cccgcggtga tctcctcgat cgcgtatgtg gatcccggca
     5281 atttcgccac caacatccag gcgggggcgg gcttcggcta tacgctgctc tgggtcgtgc
     5341 tgtcggcgag cttgctcggc atgttgtttc aggcgctgtc cgccaagctc ggcatcgtca
     5401 ccgggcgcaa ccttgccgag atgtgccgcg atcattttcc ccgccgcatg gtgtacggca
     5461 tgtggggcgt gagcgagctg gccgccatgt ccaccgacct ggccgagttt ctcggcgggg
     5521 cgctcgccat cgcgctgttg ttcgacctgc cgctgatggt cggcatggtg atcaccggca
     5581 tcgccaccta cggcatgttg ctgttggaga agaagggctt ccgtccgatg gagatcgtca
     5641 tcggcgtgcc ggccctggtg atcggggttt gctacctgac cgaattgctg atcgcgtccg
     5701 tcgcctgggg cgaggtggcg cgcggcgccg ttctgccgtc gatccccaac gccgcggcgc
     5761 tgaccatcac ggtgggcatc atcggcgcca ccgtcatgcc gcacgcgctt tatctgcact
     5821 ccggtttgac tcagaaccgc gcccccgccc gcaccgaagg cgagcggaaa aagctgctgc
     5881 gcttctccaa ccgcgaggtc gtcgtcgctt tgtcgttcgc gggattggtg aacatggcga
     5941 tggtgatcat ggccgccgcc gccttccatg ccggccacag cgaggtcgcc gaaatccaga
     6001 ccgcctatca cacgctgacg ccgctgctcg gcggcggcgc ggccgtcatc ttcctggtgt
     6061 cgctgctggt ctccggcctg tccagttcga ccgtgggcac gatggcgggc cagatcatca
     6121 tgcagggctt cgtcggcttc cgcattccgg tctgggtgcg ccggctggtg acgatgctgc
     6181 cggcgttcgc gatcgtcgcc atcggcgtcg acccgacccg cgcgctggtg atgagccagg
     6241 tcgtgctttc cttcgccttg ccgctgccga tgctggcgct ggtgtggttc acgcgcagcc
     6301 ccgccttcat gggcggcttc gccaacggcc gcctgaccga cgccgtcgcg atcgcgggca
     6361 cggccgcggt gctgatcttc aacggcgtgc tgctggccca ggctttcggc atcgcctagt
     6421 gtcccgattc cgaaattcgc ccgcagccga tatagagatg gggcgaattt cggaatcgaa
     6481 aggacactag caacctattg aatcctagta tcgttttggt ttcgaagttc gcttgcagcc
     6541 cgatagtgcg gatggtgcga acttcgaaac cacgacacta gaagatcgcc gcgcctcggc
     6601 gcggaacgcg ggtgggcgcg catcaggcag ccaaatccgg atcgcccggc ttatgcagcc
     6661 gcgccgcact ggccgtaatc gtccaatata acggatatgg tcaataaacc gatcaaactg
     6721 cctagtatat tggagcaaaa tgcaggccgc cttgagtttt cggttgtgta tatgactgcc
     6781 ggcgactaat ccccgctcaa gaacacacca accaaccgac ggttcacccc aaggggactg
     6841 cgtcgggccg gagccaggag atagtgatga tgcccgcaat gccgatgatg aacatgggtg
     6901 gcatgaacat gatgccccag atgccgatga tgaacatggg cggcatgccg atgatgccgc
     6961 aaatgatgcc gatgatgatg ccaatgatgg catgcgacat gagttgcgaa atgtcgggca
     7021 attccatggt ttgcaagatg accccgatgg gcggcatgac ggctgaaatg atgaagcagt
     7081 gctgcgagca gatgaacgcc atgatgaaga tgggaatgcc catcatgatg atgtgcggca
     7141 acatgtgcat gtgctgcatg cccgaagccg ggaagtagaa acgcctagcc ggcgatcagg
     7201 tcggtgtagc tcatcaccag gtacatgatg gctttttcag cgctggcgtt cctgtaaata
     7261 tgcggaacgt cagcctgaaa
//
LOCUS       DASWPU010000167         4475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_105431,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000167 DASWPU010000000
VERSION     DASWPU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105431"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /gene="priA"
                     /locus_tag="VGC27_03810"
     CDS             complement(<1..1171)
                     /gene="priA"
                     /locus_tag="VGC27_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY0281733.1"
                     /translation="MNDRTHMPGDAPNERAGVLLPLPLPPYDYKLPCGVALRRGLVVA
                     APLGPRQSLGVVWGKAEGAVGDNRLKEAVPLDGEPVLPAGLCDFVDWVAQYTLTPPGL
                     VLAMALRSRQAFEAEIPRLAYAKADSLPKRMTPARRRVLEIAADGLARSVPQLAEEAD
                     ATPAVVRGLIEAQALVEVELPEFPPFQAPDPDFAPPKLNTEQDLAAHLLRGGVGFHRF
                     AVSLLDGVTGSGKTETYFEAVAEALRQGKQTLILLPEIALTVQFLDRFAERFGARPAE
                     WHSDLTQKERRRVWRAVLRGEARVVVGARSALFLPFAALGLIIVDEEHEQAYKQEDGA
                     IYHARDMAVVRGRIEHCPVVLASATPSLETYVNATSGRYAHLRLHRRHGVAIMPEV"
     gene            1218..1931
                     /locus_tag="VGC27_03815"
     CDS             1218..1931
                     /locus_tag="VGC27_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281734.1"
                     /translation="MIRSGESAPSLDPAQAAGEAVKAYVRMNRERLAADGELLALLLP
                     ERFASAEVRDLQRFAIERLTAENRALKIECDALKGSQDRSARLGDGVRRAVLDLIEAA
                     SFGEVVAVATSAASVFGADKAVLCVEGADGIAASGWEGVRLIGPGTVTAVLGPGGSGA
                     ILSGGGELLFGPGGGAYKSLAAFRLRLGGGALYVLGAEAEGCFEGREIEADLGYFARG
                     LERAIRAKLIEKCPYPPKA"
     gene            1907..2824
                     /locus_tag="VGC27_03820"
     CDS             1907..2824
                     /locus_tag="VGC27_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY0281735.1"
                     /translation="MPLSAESLKQAWLASLAHERRASPHTLRAYGDDLHRFLSFLREH
                     KGRTPDERMLAKLAPADIRAFITLRRGQNLGARGVQRALAAVRSFFRYLAREEILENA
                     APRAIRTPRIPRTLPRPLSIADAARTLDEAGENDVEWLGARDAALLTLLYGAGLRISE
                     GLSLKRGDVPLGSSLSVVGKGRKERSVPILPVVREAAERYVALIPFAGARDAPLFLSR
                     RGKAMSAREAQALMQRLRSRLGLGERATPHALRHSFATHLLANGGDLRAVQELLGHAS
                     LSTTQTYTEIDAKKMMDVYAKSHPRSSIA"
     gene            2930..>4475
                     /locus_tag="VGC27_03825"
     CDS             2930..>4475
                     /locus_tag="VGC27_03825"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY0281736.1"
                     /translation="MTGVRSNIEIARAAKLKPIADIGLKAGIPEDALLHYGPHKAKLS
                     YDFIESLKTRPDGKLILVTAITPTTAGEGKTTTTVGLTDGLNRLGKHAMACLREPSLG
                     PCFGMKGGAAGGGYAQVVPMEDINLHFTGDFHAVTSANNLLAAMIDNHLYWGNALGVD
                     PRRIAWRRVIDMNDRALRSIVGSLGGVANGFPREDGFDITVASTVMAVLCLATDLKDL
                     ERRLGNIHIGQTREHKNVTARELNASGAMAVLLKEALMPNLVQTLENNPVFVHGGPFA
                     NIAHGCNSVVATTTALKLADYVVTEAGFGADLGAEKFFDIKCRKAQLKPSATVLVATA
                     RALKMHGGVGRDDIKHENVAAVAAGCSNLVRHIDNLKKFGVPVIVAINHFTGDSEAEI
                     AAIRQASEARGCKAVLCMHWAEGGKGAEELAREVVLVCEAGAANFQPLYPDHMPLWDK
                     LRMIATSIYDAGEITADRNVRANIEEMQSQGFGHFPICIAKTQYSFSTDPNLKGAPSG
                     HRLHVRD"
BASE COUNT          773 a         1479 c         1481 g          742 t
ORIGIN      
        1 gaacctccgg catgatggcg acgccgtggc ggcgatgcag gcgaagatgc gcgtagcgcc
       61 cgctcgttgc gttcacataa gtttccagcg acggcgtggc gctggccagc accaccggac
      121 aatgctcgat ccgtccgcgc accaccgcca tgtcgcgggc atgatagatg gcgccgtctt
      181 cctgcttata ggcttgctca tgctcttcat cgacgatgat gagcccgagc gcggcgaaag
      241 gtaggaacag ggccgaacgc gcgcccacca ccacccgcgc ttcaccgcgc agcaccgcgc
      301 gccacacccg gcggcgttcc ttttgcgtga ggtccgaatg ccattcggcg gggcgggcgc
      361 cgaagcgttc ggcgaagcgg tcgaggaact gcaccgtcag cgcgatctcc ggcaacagga
      421 tcagggtctg cttaccctgg cgcagcgctt cggccaccgc ctcgaaatag gtttcggtct
      481 tgcccgatcc ggtcaccccg tcgagcaggc tgaccgcgaa gcgatggaag cccacgccgc
      541 cgcgcaacag atgcgccgcc agatcctgct cggtgttgag cttgggcggc gcaaaatcgg
      601 gatcgggggc ctggaacggc gggaactcgg ggagttcgac ttcgacgagc gcttgcgcct
      661 cgatcagccc gcgcaccacc gccggcgtcg cgtcggcttc ttcggcgagc tgcggcacgg
      721 agcgcgccaa tccgtccgcg gcgatctcca gcacgcgccg gcgcgcgggc gtcatgcgtt
      781 tgggcaagct gtcggctttg gcataggcga ggcgcgggat ctcagcctcg aaagcctggc
      841 gcgagcgcag cgccatggcc agcaccagcc ccggcggggt cagcgtgtac tgtgccaccc
      901 aatcgacgaa atcgcacagc ccggcgggca gcaccggttc gccatcgagc ggcacggctt
      961 ctttcagcct attgtcgccg accgcgcctt cggctttgcc ccacaccacg cccaggcttt
     1021 ggcgtgggcc gagcggcgcc gccaccacca gccctcgtct cagagcaacg ccgcacggca
     1081 gcttgtagtc gtaaggcggc agcggcagag gcagcagaac gcccgcccgt tcgttcgggg
     1141 catcgccagg catgtgcgtt cggtcgttca tgcgttacac tttgagttgc gattctcttt
     1201 ggtcaaccca tattgccatg atccgcagcg gcgaaagcgc accatcttta gacccggccc
     1261 aagcggcggg ggaggcggtc aaagcctatg tccggatgaa tcgggagcgg ctggcggcgg
     1321 acggcgaact gttggcactg ctgttgccgg agcgcttcgc cagcgccgag gtgcgcgact
     1381 tgcagcgctt cgccatcgag cggctgacgg ctgagaaccg ggcgctgaaa atcgaatgcg
     1441 atgcgctgaa gggttcccag gaccgtagcg cgcgtctggg cgacggcgtg cgccgcgcgg
     1501 tgctcgatct catagaggcc gcaagcttcg gcgaggtcgt cgccgtggcg accagcgcgg
     1561 cctccgtctt cggtgccgac aaggccgtgc tttgcgtcga gggcgcggat ggcatcgcgg
     1621 cgagcggatg ggaaggcgtg cgtctgatcg gtcccgggac ggtgacggcc gtgctcgggc
     1681 cgggcggctc gggggccatt ctgtcgggcg gcggcgagct tttgttcggc ccgggcgggg
     1741 gcgcgtacaa aagcctggcc gcgttccgcc tgcgccttgg cggcggcgcg ctctatgttc
     1801 tgggcgcgga ggccgaagga tgctttgaag ggcgggagat cgaagccgat ctcggctact
     1861 tcgcgcgcgg gctggaacgg gcgattcgcg ccaagctgat cgaaaaatgc ccctatccgc
     1921 cgaaagcttg aaacaagcct ggctggcgtc gctggcccat gagcgacgcg ccagccccca
     1981 cacgctgcgc gcttacggcg acgatcttca tcgcttcctt tccttcctgc gcgagcacaa
     2041 gggccgcacg cccgacgaac gcatgctggc gaaactggcg cccgcggaca tccgcgcgtt
     2101 catcactctc agacgcggcc agaatttggg cgcgcgcggg gtgcaacggg cgctggcggc
     2161 ggtgcgcagc ttcttccgct atctggcgcg cgaagagatt ctggagaacg ccgcgccgcg
     2221 cgccatccgc accccgcgca tcccgcgcac cttgccgcgg ccgctcagca tagcggacgc
     2281 cgcgcgcacc ctcgatgaag ccggcgaaaa cgatgtcgaa tggctgggcg cgcgcgatgc
     2341 ggcgctgctc acgctgctct acggcgcggg cctgcgcatt tcggaaggct tgagtctcaa
     2401 gcgcggcgac gttccgctcg gttcttcgct tagcgtagtc ggcaaaggtc gcaaggaacg
     2461 cagcgtgccc atcctgccgg tggtgcgcga ggcggcggag cgctacgtcg cgctgattcc
     2521 gtttgcgggc gcgcgcgacg cgcccttgtt tctctcccgc cgcggcaaag cgatgagcgc
     2581 gcgcgaggcg caggccttga tgcagagact gcgctccagg ctcggtctcg gcgaacgcgc
     2641 cacgccgcac gcactgcgcc attccttcgc cacccatctg ctcgcgaacg gcggcgatct
     2701 gcgcgcggtg caggaattgc tcggccatgc ctcgctctcc accacccaga cctacaccga
     2761 aatcgacgcc aagaagatga tggacgttta cgctaagtcc catccgcgca gcagcattgc
     2821 gtaaggccga gttgtgcaaa caagcaattg gcgtctaatt cccgagccgc aggaatcgcg
     2881 ccggacagct taacgccgcg atccaagtgc gtgtgaaacg gagtttcgca tgacaggtgt
     2941 acgcagcaat atcgaaatag cccgcgccgc gaaattgaaa ccaatcgccg atatcggcct
     3001 caaggcgggc attcccgagg atgcgctgct gcactacggc ccgcacaagg ccaagctcag
     3061 ctacgatttt atcgagagct tgaagacgcg cccggacggc aagctgatcc tggtcaccgc
     3121 catcacgccc accaccgcgg gcgaaggcaa gaccaccacc accgtcggcc tcaccgacgg
     3181 gctgaaccgc ctcggcaagc atgccatggc ctgcctgcgc gagccttcgc tcgggccgtg
     3241 tttcggcatg aagggcggcg ccgccggcgg cggctatgcc caggtcgtgc cgatggaaga
     3301 catcaacctg catttcaccg gcgatttcca tgccgtcacc tcggccaaca atctgcttgc
     3361 cgcgatgatc gacaaccatc tttactgggg caacgcgctg ggggtcgatc cgcgccgcat
     3421 cgcctggcgc cgcgtcatcg atatgaacga ccgtgcgctg cgttcgatcg tgggttcgct
     3481 cggcggcgtc gccaacggct ttccgcgcga ggacggcttc gacatcacgg tcgcttccac
     3541 ggtgatggcg gtgctctgcc tggcgaccga tctgaaggat ctggaacgcc gtctcggcaa
     3601 catccatatc ggccagaccc gcgagcacaa gaacgtcacc gccagagagc tgaacgcttc
     3661 gggcgcgatg gccgtgctgt tgaaggaggc gctgatgccc aatctggtgc agacgctgga
     3721 gaacaatccg gtgttcgtgc atggcgggcc gttcgccaat atcgcccatg gctgcaactc
     3781 ggtggtcgcc accaccaccg cgctcaagct cgccgactat gtggtgactg aggccgggtt
     3841 cggcgccgat ctcggggccg aaaagttctt cgacatcaaa tgccgcaagg cgcagctcaa
     3901 gccgtccgcc acggtgctgg tcgccaccgc gcgcgccttg aagatgcatg gcggggtggg
     3961 gcgcgacgat atcaagcatg aaaacgtcgc tgccgtcgcc gccgggtgca gcaatctggt
     4021 gcgccatatc gacaatctga agaagttcgg cgtgccggtg atcgtggcga ttaatcattt
     4081 caccggcgac agcgaggccg agatcgcggc gatccgccaa gcctccgagg cgcgcggctg
     4141 caaggcggtc ttgtgcatgc actgggccga aggcggcaag ggcgccgagg aactggcgcg
     4201 cgaggttgtt cttgtgtgcg aagccggcgc ggcgaatttc cagccgcttt atcccgacca
     4261 tatgccgctg tgggacaagc tgcggatgat cgccaccagc atctatgacg cgggcgaaat
     4321 caccgccgac aggaatgtgc gcgcaaacat cgaggagatg cagagccagg gcttcggcca
     4381 tttcccgatc tgcatcgcca agacgcaata ttccttttcc accgatccga atctcaaggg
     4441 cgcgccgagc ggccatcggc tgcatgtgcg cgacg
//
LOCUS       DASWPU010000168         4524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_56414,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000168 DASWPU010000000
VERSION     DASWPU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56414"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /gene="mreD"
                     /locus_tag="VGC27_03830"
     CDS             complement(<1..333)
                     /gene="mreD"
                     /locus_tag="VGC27_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY0281737.1"
                     /translation="MGGRFLASIVPVSCGVICVFAANLPFSFMGPWVPSPLYALMPVY
                     FWCLVRPDLMSPAWAFLIGVLHDILSGGPPGVWAASFVATYAVIDRQRDAFAGLSGVA
                     ALLGFATAA"
     gene            complement(363..1346)
                     /gene="mreC"
                     /locus_tag="VGC27_03835"
     CDS             complement(363..1346)
                     /gene="mreC"
                     /locus_tag="VGC27_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY0281738.1"
                     /translation="MAVLAVALILIGKAQSSLFDRARANVMDWMEPTLSASREPMVSF
                     TRWVGSIGEIFAVYQQNLKLKEENARLRQWHNTAISLNERLKRYKLLLNAVPDPALSQ
                     VVARVIGRASHPFMQTLILDAGKSHGVKPGQAVVDSRGMIGRIFLTGERTSWVILLTD
                     LNSRIPVTIEPGNAQAIMAGDNSPAPMIETLSRGTPLKVGDQVVTSGDGDILPAGLAI
                     GSIAVVGNTYRVALFADETATQDVIILDFKQPPEQPPSPSPGDLPVTAAGLPPAASPP
                     QQPAAASPTVKPSAAPPARSNVKPQTPTTQDATPAAPAGNPDNDVSDVGNE"
     gene            complement(1447..2487)
                     /locus_tag="VGC27_03840"
     CDS             complement(1447..2487)
                     /locus_tag="VGC27_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010442888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY0281739.1"
                     /translation="MFGSLLGALSSDMAIDLGTANTLVYVKGRGIVLNEPSVVATINR
                     GGKKQVRAVGNDAKMMLGRTPGNIEAIRPMRDGVIADFEVAEEMIKHFIRKVHNRRSF
                     ANPMIIVCVPSGSTAVERRAIQESALSAGARRVFLIEEPMAAAIGAGLPVSEPTGSMV
                     VDIGGGTTEVAVLSLGGIVYSRSVRVGGDKMDEAIIAYIRRHQNLLIGEASSERIKKE
                     IGSAAPPVDGNGVTLEIKGRDLLNGVPKEITITQRHIAEALAEPVGAIVEAVKVALEA
                     TPPELAADIVDKGIVLTGGGSLLANLDQVLREETGLPVSIADDPLSCVALGTGRVLEN
                     TKMMRHVLTTAF"
     gene            complement(2587..4149)
                     /locus_tag="VGC27_03845"
     CDS             complement(2587..4149)
                     /locus_tag="VGC27_03845"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY0281740.1"
                     /translation="MSTDKVKSERVIIFDTTLRDGEQSPGAAMTLGEKIEVAEFLDAM
                     GVDVIEAGFPISSPGDFEAVTEVSKRVKNASVCGLARAGFKDIDRAGEAIKTAKRPRI
                     HTFISTSPVHMKHKLNMGANAVLDAVGASVAHARGLTDDVEWSAEDATRTERDFLCRC
                     VELAIRSGATTINIPDTVGYATPQEYFELVTMLRERVPNADKVIFSTHCHNDLGMAVA
                     NSLAGVLAGARQVECTINGIGERAGNAALEEIVMALKVRNDRMPFATGIKAELLARAS
                     KLVSNVTNFPVQYNKAIVGKNAFSHESGIHQDGMLKNVETYEIMRPEDVGVKETSLVL
                     GKLSGRHAFRDKLANLGYELADDAFADAFRRFKEIADKKKTMFDEDIVALVDDGIVRG
                     QDPITVKSLRVETGKDVAPSADLTLGVNGEEKHTKTTGDGAVDAIFNAIHELYPHTAG
                     LQLYQVHAVTEGSDAQATVSVRLEENGRTVTGRGADTDTLVASAYAYVNALNKLLVKR
                     NKQVPETLNVAQ"
BASE COUNT          791 a         1484 c         1391 g          858 t
ORIGIN      
        1 cgccgccgtg gcgaaaccca gcaaagcggc cacgcccgac agcccggcga aggcgtcgcg
       61 ctgccggtcg atgacggcat aggtggcgac aaaggacgcg gcccagacgc cgggcggccc
      121 gccggacaga atgtcgtgca agacgccgat cagaaacgcc caggccggag acatcagatc
      181 aggccgcacc agacaccaga aataaaccgg catcagcgcg tacaagggcg agggaaccca
      241 cggacccata aacgaaaacg gcagattggc cgcaaacacg cagatcacgc cgcatgatac
      301 gggaacgatg cttgcgagga acctgccgcc catgatgtta cccacgcgct ccacggccgg
      361 cctcactcat tgccgacgtc cgagacgtcg ttgtccggat tgccggccgg cgcggccggg
      421 gtagcgtcct gggtcgtcgg ggtttgcggt ttgacgttgc tcctggccgg cggcgccgca
      481 cttggcttga ccgtcggcga ggctgcggcc ggctgttgcg gcggcgaagc ggcgggcggc
      541 agtcccgcgg ccgtgaccgg caaatcgcct ggcgagggtg acggcggctg ttcggggggc
      601 tgtttgaaat cgaggatgat cacatcctgg gtcgcggtct cgtcggcgaa cagcgccacc
      661 cgataagtgt ttccgacgac cgcgatggac ccgatcgcca agcccgccgg caggatgtcg
      721 ccatcgccgg acgtgaccac ctggtcgccc accttcagcg gcgtgcctcg cgacaaggtt
      781 tcgatcatcg gggcgggcga attgtctccc gccatgatcg cctgggcatt gcccggctcg
      841 atggtgacgg gaatgcggct gttcaaatcg gtgagcagga tgacccagga ggtgcgttca
      901 ccggtgagaa agatgcggcc gatcatgccg cgcgagtcga ccaccgcctg gcccggctta
      961 accccatgcg acttgccggc gtcgagaatc agcgtttgca tgaaaggatg gctggcgcgg
     1021 ccgatcaccc gcgccacgac ttgagacagc gccggatcgg gcacggcgtt gagcagaagc
     1081 ttgtagcgct tcagccgctc gttcaaggaa atcgccgtgt tgtgccactg gcgcaagcgc
     1141 gcgttttctt ccttgagctt caagttttgc tgatagacgg cgaagatctc gccgatcgaa
     1201 ccgacccagc gggtgaagct caccatcggc tcgcggctgg ccgacagcgt cggctccatc
     1261 cagtccatga cattggcgcg ggcgcggtcg aacaacgagg actgcgcctt gccgatcagg
     1321 atcagcgcca cagcgagcac cgccacgatg atcagcggga ggtgcgcgcc cccacgcgtt
     1381 cgcgcaattc tccaggtccc gttcgccata ctctacccaa cccgcgccgt ctgcccccgt
     1441 tcatgcctag aaggcggtcg tcaacacgtg ccgcatcatc ttggtgtttt ccagcacccg
     1501 cccggtaccg agcgccacgc atgacaaggg atcgtcggcg atggatacgg gcaggcccgt
     1561 ttcctcgcgc agcacctggt cgagattggc gagcagcgaa ccgccgcccg tcagtacgat
     1621 gcccttgtcc acgatgtcgg cggccagttc cggcggcgtc gcctcgagcg caaccttcac
     1681 cgcttcgacg atggccccta ccggctcggc caaagcctcg gcgatgtggc gctgggtgat
     1741 ggtgatttcc ttcggcacgc cgttgagcag atcgcgccct ttgatttcga gggtcacgcc
     1801 gttgccgtcg accggcggcg ccgccgaacc gatttccttc ttgatgcgct cggagctggc
     1861 ttcgccgatc agaaggttct gatggcgacg gatataggcg atgatcgcct cgtccatctt
     1921 gtcgccgccg acgcgcaccg agcgcgaata gacgatgccg ccgagcgaca gcaccgccac
     1981 ttccgtggtg ccgccgccga tatcgacgac catcgagccg gtcggctcgc tgaccggcag
     2041 gccggcgccg atcgccgccg ccatcggttc ctcgatcagg aagacgcgcc gcgcccccgc
     2101 cgacagcgcc gattcctgaa tggcgcgccg ctccaccgcg gtcgagcccg acggcacgca
     2161 gacgatgatc atcggattgg cgaaggagcg gcggttatgc accttgcgga tgaaatgctt
     2221 gatcatttcc tccgccacct cgaaatccgc gatgacgccg tcgcgcatcg gccggatcgc
     2281 ctcgatgttg cccggcgtcc tgcccagcat catcttggcg tcgttgccga ccgcgcgaac
     2341 ctgtttcttg cctccgcgat tgatcgtggc gacgaccgag ggttcgttca ggacgatgcc
     2401 gcgccctttc acgtaaacca gggtgttcgc ggttccaaga tcgatcgcca tatcgctcga
     2461 cagtgcgccg agaagactgc cgaacatgct taacctgcca tttcttttct aacttgacac
     2521 catgccgtgc aattgccgtt cccgcaaaaa ccggccccgg cggacccgcg ccggagccgg
     2581 tttgtttcat tgtgcaacat tcaaggtttc gggcacctgc ttattccgtt tcaccaagag
     2641 tttattaaga gcgttaacat aggcataagc ggaggccacc agcgtgtcgg tgtccgcccc
     2701 cctgccggtt accgtacgtc cgttttcctc gagcctgacg ctcaccgtgg cctgcgcgtc
     2761 cgatccttcg gtcacggcat ggacctggta aagctgcaaa ccggcggtgt gcggataaag
     2821 ctcgtgaatg gcattgaaga tcgcatcgac cgcgccgtcg cccgtggtct tggtgtgctt
     2881 ttcctcgccg ttcacgccca gcgtcagatc ggcgctcggc gcgacatcct ttccggtctc
     2941 gacgcgcaac gacttgaccg tgatcggatc ctggccgcgg acgatcccgt cgtccaccag
     3001 ggccacgata tcctcgtcga acatggtctt cttcttgtcg gcgatttcct tgaagcgcct
     3061 gaacgcgtct gcgaaggcgt cgtcggcaag ctcgtagccc agattcgcca gcttgtcgcg
     3121 gaaggcgtga cggccggaga gcttgcctag caccagcgag gtctccttca cgcccacatc
     3181 ctccgggcgc atgatctcgt aggtctccac gttcttcagc atgccgtcct gatggatgcc
     3241 ggattcgtgg ctgaaggcgt tcttgccgac gatcgccttg ttgtactgca ccggaaaatt
     3301 ggtgacgttg gagaccagct tggaggcgcg ggccagaagc tcggccttga tgccggtggc
     3361 gaacggcatg cggtcgttgc gcaccttcag cgccatcacg atctcttcca gcgccgcatt
     3421 gcccgcgcgc tcgccgatgc cgttgatcgt gcattcgacc tggcgcgcgc cggcaagcac
     3481 accggccagg gaattggcca ccgccatgcc caaatcgtta tggcaatggg tggaaaagat
     3541 caccttgtcg gcgttgggca cgcgttcgcg cagcatcgtg acaagctcga aatattcctg
     3601 cggcgtcgca taaccgaccg tatcgggaat gttgatcgtg gtggcgccgg aacggatcgc
     3661 cagttccaca caacggcaga ggaagtcgcg ttcggtgcgc gtagcgtcct ccgcgctcca
     3721 ttccacatcg tcggtcaggc cgcgcgcatg cgcgacggac gcgcccaccg catcgagcac
     3781 cgcgttcgcg cccatattga gcttgtgctt catatggacc gggctagttg agatgaaggt
     3841 atggatgcgc gggcgcttgg cagtcttgat cgcttcgccg gcgcggtcga tgtccttgaa
     3901 gccggcgcgg gccagaccgc agacggaggc gttcttcacc cgtttggaga cttccgttac
     3961 cgcctcgaaa tcgccgggcg aggaaatcgg aaaccccgcc tcgatgacat cgacgcccat
     4021 ggcgtcgaga aattccgcga cttcgatttt ttccccgagc gtcattgcgg cgccgggcga
     4081 ctgctcgccg tcgcgcaatg tcgtgtcgaa gatgataacc cgttccgatt tcactttgtc
     4141 tgtgctcatg gccacacgtt ccttccgttt gccgcattcc gcggcttcga tctctccttt
     4201 cgctcgtgat cccctaaaca cccggcgcga gaacgatccc gcgccattcg gcgcctaggg
     4261 gctgctaagg aggagaagcc cgtcttgcaa caaaccgagg gaaggacggg gcgtgagcag
     4321 gacgcgcgcg cagccgcgag cggaaaatcc gctctttgtt ccctgctgca tcgcgccttt
     4381 cgtcatcgcc ggatcgccgt tccgcccgcc gagttttcag ctcgctaaac ccacacgaat
     4441 cgccgttaaa cgcccgtaaa ggcattgttt tccgatccgt gcatggcgtt ttacgctgat
     4501 tgtactacgg cgcgcaatag cgcc
//
LOCUS       DASWPU010000169         4584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_68856,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000169 DASWPU010000000
VERSION     DASWPU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68856"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VGC27_03850"
     CDS             complement(<1..230)
                     /locus_tag="VGC27_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuvB-like domain-containing protein"
                     /protein_id="HEY0281741.1"
                     /translation="MAKPQPTDRLVTPQRSEDDSLESSLRPKVLAEFVGQQQARENLS
                     VFIAAAKSRAEALDHVLFSGPPGLGKTTLAQIV"
     gene            complement(231..830)
                     /gene="ruvA"
                     /locus_tag="VGC27_03855"
     CDS             complement(231..830)
                     /gene="ruvA"
                     /locus_tag="VGC27_03855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY0281742.1"
                     /translation="MIGKLTGIVDFVGEDHVLIEVGGVGYLVHCPSSTLSKLTAGAHA
                     SLTIETRLTDETIKLFGFFTAEEREWFRLLQTVQNVGARVALNVLSALSPRDLERALA
                     LGDKAVIGRAQGVGPKLALRIVTELKDKAPAMMLRGHAGEAFAAAPRGPEADAVAALL
                     KLGYSQGVAAEAVARATRDLGEGAALDALIREALKGMGR"
     gene            complement(827..1333)
                     /gene="ruvC"
                     /locus_tag="VGC27_03860"
     CDS             complement(827..1333)
                     /gene="ruvC"
                     /locus_tag="VGC27_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY0281743.1"
                     /translation="MQTVRILGLDPGLGRMGWGVIAVSGTRLSHVAHGVIVTRPADGL
                     GLRLLMLHGEVARIIAAFEPGAIAVEQAFVHKDPSAALKLGHARAVALLAAAQAGLEI
                     AEYAPNHIKKSVVGAGHADKRQIQMMVRRLLPACGVEAGDAADALAAAITHAHLAGTR
                     ARIMAALA"
     gene            complement(1411..3081)
                     /gene="ybaL"
                     /locus_tag="VGC27_03865"
     CDS             complement(1411..3081)
                     /gene="ybaL"
                     /locus_tag="VGC27_03865"
                     /inference="COORDINATES: protein motif:HMM:NF007950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HEY0281744.1"
                     /translation="MVHESPLIAVIVIGLGLAFFLGTLANRLKLPPIVGYLVAGVLVG
                     PFTPGFIADQDLTLQLAEIGVILLMFGVGLHFSLKDLLSVKAIAIPGAVVQIVVATLL
                     GMSLAWVLGWPVGGGLVFGLALSVASTVVLLRALQDRRLIETERGRIAVGWLIVEDLA
                     MVLALVLLPAIAGLLKDGGNATPVGTSHIISALLITVGKVTAFVAVMLVIGRRVIPWI
                     LHYIAHTGSRELFRLSVLVIALGVAYGAAVLFDVSFALGAFFAGMILSESPLSQRAAE
                     ETLPLRDAFAVIFFVSVGMLFNPHILLSDPLPVLATFLIIVLGKSVAAFFIVRAFGHP
                     TATALTISASLAQIGEFSFILANLGLSLGLMPVEARDLILGTSILSILLNPLVFAGVG
                     LLTKRIAPEDAAGKDVKHVPIAVPLAPTHLSSHAVLVGYGRVGMLVAEGLRAARVPVL
                     IIEDAPDALEHLEASGYEFLHGNGADERVLAAANLPGAAILLVAIPEAFEADQIVEQA
                     RRINPELLIVARAHFDAQVAHLLEHGASAVVMGEREIARAMLAHVGTA"
     gene            complement(3232..3978)
                     /locus_tag="VGC27_03870"
     CDS             complement(3232..3978)
                     /locus_tag="VGC27_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0281745.1"
                     /translation="MAGHSHAKNVMFRKSKSNKERSKLFSKLAREIIVASKMGLPDPD
                     HNARLRAAVNAAKAQSMPKDNIERAIKKGQGGDAENYDEIRYEGYGPGGVALIVECLT
                     DNRTRTVADVRAAFSKYGGVLGETGSVAFMFDRVGVIAYPKDRGSDDAMLETALDVGA
                     EECNSDDEEHEFLTSPEDFAAVRDALEAKLGPPASSGIFFRPQNTVAVSDSAGETLMK
                     LIDVLDDHDDVQRVTGNYELSEALMEKLNA"
BASE COUNT          865 a         1480 c         1486 g          753 t
ORIGIN      
        1 acgatctgcg ccagcgtggt cttgcccagc cccggcggcc cggagaacag cacatggtcg
       61 agggcctcgg cgcgggactt cgccgccgcg atgaagaccg acagattctc gcgggcctgc
      121 tgctggccga cgaattcggc aaggaccttg gggcgcagcg acgattccag cgaatcgtct
      181 tcactgcgct ggggcgtaac caaacgatca gtcggttgcg gctttgccat ctagcgtccc
      241 atgcccttca aggcttcgcg gatcaacgca tccagcgccg cgccctcgcc gaggtcgcgc
      301 gtagcgcgcg ccaccgcttc ggccgcaacc ccttgagaat agccgagctt gaggagcgcc
      361 gcgacggcgt cggcttccgg cccgcgcggc gcggcggcga acgcttcgcc cgcatggccg
      421 cgcagcatca tggccggcgc cttgtccttc agctcggtca cgatacgcag cgccagtttg
      481 gggccgacgc cctgggcgcg gccgatcacc gccttgtcgc ccagcgccag cgcacgctcc
      541 agatcgcgcg ggctgagcgc ggacagcaca ttcaacgcca cccgtgcgcc gacgttctgc
      601 accgtttgca gcaagcggaa ccattcgcgc tcctcggcgg tgaagaagcc aaagagcttg
      661 atcgtctcgt cggtcaggcg cgtttcgatc gtcagggagg cgtgcgcgcc cgcggtcagc
      721 ttggacaagg tcgaagacgg gcaatgcacc agatagccga cgccgccgac ctcgatgagg
      781 acgtgatcct cgcccacgaa atccacgatg ccggtcagtt tcccgatcat gcgagcgccg
      841 ccatgatgcg cgcccgcgtg ccggccagat gggcgtgggt tatggcggcg gcgagcgcgt
      901 cggccgcgtc gccggcttcc acgccgcagg cgggcagcaa acgccgcacc atcatctgaa
      961 tctggcgctt gtccgcatgc ccggcgccga ccaccgactt cttgatgtgg ttgggcgcgt
     1021 attcggcgat ctccagcccc gcttgcgcgg cggccaagag agcaacggcg cgggcatggc
     1081 cgagcttgag ggcggcggac gggtccttgt gcacgaaggc ctgctccacc gcgatggcgc
     1141 cgggctcaaa cgcggcaatg atgcgcgcga cctcgccgtg cagcatcaga agacgcagcc
     1201 ccagcccgtc ggcggggcgg gtcacgatca cgccgtgcgc gacatgcgaa agcctggtgc
     1261 cggacacggc gatgacgccc cagcccatgc ggccaaggcc cggatcgagg ccgagaatgc
     1321 gaacggtctg cacggcaaaa cccgaacgaa tcgccgccgc cagtgtcgcg gatcatgggc
     1381 caccggcgca acaggcgtcc cccgcgccca tcaggccgtg ccgacatgcg ccagcatcgc
     1441 gcgcgcgatc tcgcgctcgc ccatcaccac ggcgctggcc ccatgttcca gcagatgcgc
     1501 gacctgggcg tcgaaatggg cgcgcgcgac gatcagcagc tcgggattga tccggcgcgc
     1561 ctgctcgacg atctggtcgg cttcaaaggc ctcgggaatg gcgaccaaca ggatcgcggc
     1621 cccgggaaga ttggccgccg ccagcacccg ctcatcggcc ccgttgccgt gaaggaattc
     1681 gtagccggag gcctcgagat gctccagggc atcgggggcg tcctcgatga tgaggacggg
     1741 cacccgcgcg gcgcgcaggc cttcggccac cagcatgccg acgcggccat agcccaccag
     1801 cacggcatgg gacgacagat gcgtcggagc gaggggcacg gcaatcggca catgtttgac
     1861 gtccttgccc gcggcgtctt ccggcgcgat acgcttggtg agcagcccca ctccggcgaa
     1921 aaccagcgga ttgagcagga tcgagaggat cgaggtgccg agaatgaggt cgcgcgcctc
     1981 gacgggcatc aggccgaggc tgaggccgag gtttgcgagg atgaaggaga attcgccgat
     2041 ctgggcgagg ctcgccgaaa tcgtcagtgc cgtcgccgtc ggatggccga aggcgcggac
     2101 gatgaaaaag gccgcgaccg atttgccgag tacgatgatg agaaaggtcg cgagcacggg
     2161 cagagggtcg ctgaggagaa tatgcggatt gaacaacatg ccgacggaga cgaagaagat
     2221 caccgcgaag gcgtcgcgca gcggcagggt ttcctccgcc gcgcgctggc tcaggggcga
     2281 ttccgagagg atcatgccgg cgaagaaggc gccgagcgcg aaggagacgt caaagagaac
     2341 cgccgcgccg taagcaacac cgagcgcgat caccagcacg ctgagacgga agagctcacg
     2401 cgagcccgta tgggcgatat agtgaaggat ccatggaatg acgcggcggc cgatcaccag
     2461 catcaccgcg acgaaggcgg tgaccttgcc gacggtgatg agcagcgccg agatgatatg
     2521 cgaggtgccg acgggcgttg cattgccgcc atccttcagc aggccggcga ttgcgggcag
     2581 cagaacgagc gccaggacca tggcgagatc ctcgacgatc agccagccga cggcgatgcg
     2641 tccgcgttcc gtctcgatga ggcggcggtc ctgaagggcg cggagcagga ccacggtgct
     2701 ggcgacggat agcgcgaggc cgaagacgag gccgccgccg acgggccagc cgaggaccca
     2761 tgcgagcgac atgccgagca gggtcgcaac cacgatctgc acgacagcgc ccggaatggc
     2821 gatggctttg acggagagaa ggtccttcag cgagaaatgc aggccgacgc cgaacatcag
     2881 caggatgacg ccgatttcgg ccagttgcag ggtcaggtct tgatcggcga taaacccagg
     2941 cgtgaacggt ccgaccagca cacctgcgac caaatagccc acgatgggcg gcagcttcag
     3001 ccggttggca agggtgccca ggaaaaaggc caacccaaga ccgatgacaa tgacagcaat
     3061 gagcggcgat tcgtggacca tgtgtcttta tatacacgaa cggcggcgat cggcccgcca
     3121 atttcgccgt ttgtcaactt ttcaatatca ctcctcaata tcactccgcc agcttcgcca
     3181 tccgtctccg cccttcgggc ttcgccggac cagcctgcca ctcctcctac atcatgcatt
     3241 caacttttcc atcagcgcct cggaaagctc gtaattgccg gtgacgcgct gcacgtcgtc
     3301 gtggtcgtcg agcacgtcga tcagcttcat cagcgtctcg ccggcgctgt cgctcaccgc
     3361 cactgtgttc tgcgggcgga agaagatgcc agaggacgcg ggcggcccca gcttggcctc
     3421 cagcgcatcg cgcaccgcgg cgaaatcctc cggcgaggtc aggaattcgt gctcctcgtc
     3481 gtcggagttg cattcctccg ccccgacatc gagcgccgtt tccagcatgg cgtcgtcgct
     3541 gcccctgtcc ttcggatagg cgatcacgcc gacgcgatcg aacatgaaag cgaccgaccc
     3601 ggtttcgccc agcaccccgc catatttcga gaaggcggcg cggacatcgg ccaccgtgcg
     3661 ggtgcggttg tcggtcaggc actccacgat cagcgcgacg ccgccggggc catagccttc
     3721 atagcggatc tcgtcgtaat tctcggcgtc gccgccctga cccttcttga tggcgcgctc
     3781 gatattgtcc ttgggcatcg actgcgcctt ggctgcattg accgccgcac gcaagcgcgc
     3841 gttgtggtcc gggtccggca agcccatttt ggacgcaaca atgatttcgc gggcaagctt
     3901 ggaaaagagc ttggagcgct ccttgttcga cttgcttttg cggaacatga cgttcttggc
     3961 gtgggaatgg ccggccatgg ggatgacttc tttttttcta agcggcgcgt gagatagccg
     4021 ggcggcgagg cggctggcaa gagcggggcc tgcgccaggc cgttggaggc gcaaagcgag
     4081 gacgccggcg ctgggcctgg gatgggcaat ttctctaagc tattgaataa tcaacatttt
     4141 ccttcaaagg ttgttttcga ctttttcgag ggcgtccccc ctccccgtgt tcgctccttc
     4201 tcaccaccct ggccttcggc agcgtttctt ccatcagcgt ggctgcctcc gcgccgcttc
     4261 gcttcccccg cgccggacgc ggcagaaccc tcggacgcca tgatacgccc gttcgccgtg
     4321 cattggataa gctatgcgtc acgaacgcga tcaggcccga taaaatcaag tattctaact
     4381 acttaattta tgttgacgcc gcgtaatacg taaaaaccga tgcgaccatt atgtcggagc
     4441 gcaacgcata agacacttta tccgcggcgt cgcgaaatct agtctgcatc gtaattcttc
     4501 ctcgccagag aaagaattgt gctaggccgg gatgcgcgcc cggtggaagg atgcatgatg
     4561 gccaaggcct cgtttatcga cgaa
//
LOCUS       DASWPU010000170         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_93161,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000170 DASWPU010000000
VERSION     DASWPU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93161"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..426)
                     /locus_tag="VGC27_03875"
     CDS             complement(73..426)
                     /locus_tag="VGC27_03875"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEY0281746.1"
                     /translation="MSAAARAALLGRCPRCGEGRLFDGYLTVAARCTKCGLDYAVFDA
                     GDGPAVFVVLIVGAIVCAGALYVEFAFSPPYWVHAVLWIPAILILTFGLLRLMKTLLL
                     MLQYRHRAGEGKLTK"
     gene            complement(423..1334)
                     /locus_tag="VGC27_03880"
     CDS             complement(423..1334)
                     /locus_tag="VGC27_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009827704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY0281747.1"
                     /translation="MAHDEVKHDYHLVDPSPWPFVGSLGAFTMLIGGVFWMNKSYTGF
                     FGLPLNGQPWIFIGGLVLILFTMAGWWRDVVTESVVRHEHTAVVKLGLRYGMVLFIAS
                     EVMFFVAWFWAYFNTAIFYNDVGLSSWPPSGIVTLDPWHLPLLNTLILLASATTVTWA
                     HHAIQHDDRKGAVQGLLLTVLLGLSFTCIQAYEYAHAPFTFGFNGAALVPFTDPAHAN
                     LSAGVGNLGAIYGSNFFMATGFHGFHVIVGTIFLAVCLFRAIAGHFTPTRHFGFEAAA
                     WYWHFVDVVWLFLFACIYVLGRGPLSS"
     gene            complement(1480..>2103)
                     /gene="cyoE"
                     /locus_tag="VGC27_03885"
     CDS             complement(1480..>2103)
                     /gene="cyoE"
                     /locus_tag="VGC27_03885"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY0281748.1"
                     /translation="GWALATLSVAVMWLFVNTLAASLLAFTVFIYIGVYTLWLKRRTP
                     QNIVIGGLSGALPPAIGWAAVTGSLSLAPIVLVAIIFLWTPPHFWSLALTRSEDYARA
                     GVPMMPVARGKRSTRIQIMAYALVLVPLGLLPAATGIGGALYLAAAAGLGGLFLWNAL
                     AVLRETDEAGEPAAHRTFAVSIFYLFFLFAALIAERLLGFAPLHLWM"
BASE COUNT          480 a          674 c          672 g          277 t
ORIGIN      
        1 tccccttccc tcgccgcgcg tccatacgct gtcgctatgg actgcgcggc tcgccgggga
       61 tgacagcttt tgttatttcg tcagcttccc ctcgccggcg cggtggcggt attgcagcat
      121 cagcagcagg gttttcatca agcgcaagag gccgaaggtc aggattaaaa tggccggaat
      181 ccacagcacc gcatggaccc aatagggcgg cgagaaggcg aactcgacat acagcgcccc
      241 ggcgcaaaca atcgcgccca cgatcagaac gacgaatacg gccggaccgt cgcccgcgtc
      301 gaacaccgcg taatcgaggc cgcatttcgt gcagcgcgcg gcgaccgtca gatagccgtc
      361 gaacagcctg ccctcgccgc agcgcggaca gcggcccaga agcgcggcgc gcgcggcggc
      421 gctcacgagc taagcggccc ccggccaagc acgtaaatgc aggcgaacag gaacagccag
      481 accacgtcga cgaaatgcca gtaccaggcg gcggcctcga agccgaaatg gcgcgtcggc
      541 gtgaaatgcc cggcgatggc gcggaacaag caaacggcga ggaagatggt gccgacgatg
      601 acgtggaaac cgtggaagcc cgtcgccatg aagaagttcg agccgtagat ggcgccgaga
      661 ttgcccacgc ccgccgaaag gttcgcatgg gccggatcgg tgaagggcac cagcgccgcg
      721 ccgttgaaac cgaaggtgaa gggcgcgtgg gcgtattcat acgcctggat gcaggtgaag
      781 gagaggccga gaagcacggt gagcaaaagg ccctgcaccg cgcccttgcg gtcgtcgtgc
      841 tggatggcgt gatgggccca ggtcacggtg gtggcggagg ccagcaggat cagcgtgttg
      901 agcaacggga ggtgccaggg gtcgagcgtg acgatgccgg acggcggcca gctcgacagc
      961 ccgacatcgt tatagaagat cgcggtgttg aaataggccc agaaccaggc gacgaagaac
     1021 atcacttcgg aagcgatgaa caggaccatg ccgtagcgca ggcccagctt caccaccgcc
     1081 gtgtgttcgt ggcgcacgac agattcggta acgacgtcgc gccaccagcc cgccatcgtg
     1141 aacaggatca gcaccagccc gccgatgaag atccagggct gaccgttcaa cggcaatccg
     1201 aagaacccgg tgtagctctt gttcatccag aacacgccgc cgatcagcat ggtaaaggcg
     1261 cccagcgagc cgacgaacgg ccacggactg ggatcgacca gatgatagtc gtgcttcact
     1321 tcatcatgcg ccatgaccgg cctcatccat gcggattacg ggtgaacccg caacaacgtc
     1381 gtcagataga acagaaccgc caagccaaac agaatcagcg ccagcgccac attgcgccgc
     1441 ttgcgttcgc gtgcgtcgcg gtcttcgtcg gccatcgcgt cacatccaca ggtgcaaggg
     1501 tgcgaaaccg aggagacgtt ccgcgatcag cgccgcaaac aaaaaaaaca gatagaagat
     1561 cgagaccgcg aaggtgcggt gcgcggccgg ttctccggct tcgtccgtct cgcgcaacac
     1621 ggccagcgca ttccacagga aaagcccgcc cagccccgcc gccgccgcca gatacaacgc
     1681 tccgccgatc ccggtcgcgg cgggaagaag cccgagcggc accagcacca gcgcataggc
     1741 catgatctga attcgcgtcg agcgcttgcc gcgcgccacg ggcatcatcg gcacgccggc
     1801 gcgcgcgtaa tcctcggagc gggtgagcgc cagcgaccag aaatgcggcg gcgtccacag
     1861 gaagatgatc gcaaccagaa caatcggtgc gagcgaaagc gatcccgtca cggcagccca
     1921 gccgatggcc ggcggcagcg cacccgacaa tccgccgatc acgatgttct gcggcgtgcg
     1981 gcgcttcagc cagagcgtat agacgccgat atagatgaag acggtgaagg ccagcagcga
     2041 cgccgccaaa gtgttcacga acagccacat cacggccacg gacaaggtcg ccagcgccca
     2101 gcc
//
LOCUS       DASWPU010000171         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_68880,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000171 DASWPU010000000
VERSION     DASWPU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68880"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..362)
                     /locus_tag="VGC27_03890"
     CDS             complement(105..362)
                     /locus_tag="VGC27_03890"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY0281749.1"
                     /translation="MSSKAKSEAYSVDMLSFEAALKELEGIVARLEQGQVDLEDSIAL
                     YERGQALKAHCESKLKAAESRLEKIVLGDSGPAGAEPAELG"
     gene            1136..>3353
                     /locus_tag="VGC27_03895"
     CDS             1136..>3353
                     /locus_tag="VGC27_03895"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0281750.1"
                     /translation="MTIRSRGQGFTRSASTSASGGFYFSGLPSGAYEVTVEADGYRAE
                     SIANARVSASAATTYSIVLRSTTAVTGSAETVVVTGTRQTFDFTNTTTGATLNIPDLV
                     KDIPVSRDLTSIILLAPGTVGGDRSFGRRSEGYLPSIGGSSVAENAYYINGLNITDFN
                     HYTGGSLVPFDFYESVEVKTGGYPAEFGRATGGVVNAVSKSGSNEFKAAVHVNWEPDA
                     LRETAPDTYTARNKLDHESAFNTVLELGGPIIKDHLFFYGLMQLNQNSTSDSGIISQL
                     TTKTSTNSPFWGAKIDGYITDDHHLEFTYFNTKRITTLKNYAFDAATDETAVDSGLAR
                     EEWTGGESWVAKYTGRITDWLTISAAYGDNRDRDEQRGGQGITYIADQTVGGLVCGGI
                     TGGFCGSQNTTVYDIPQTTKRTFYRVDVDVLFNLLGDHHVRFGMDNESNYLAHTAIRP
                     GPEGYAVIYRQCATRADLPVANWNARCRAPAPSTAALYAWNQQFVEFNFYNTGGEFRS
                     RNTAFYVQDEWKVLDNLTVNLGVRIDGFNNFTADGSQYVALHGLFAPRAGFSYSPWED
                     DSGRFYGSFGDYYLPVAGNTAFRQGARELYFQEYWFFNGLEQNNYPSLTTQLVGWRGA
                     NACPGALTPGSSVGTVGCTVTSDGSVQDPTSSLAKNLKATREQEIIFGYEQKLDGLLS
                     GFGDLWTVGINYTRRDLMVTAEDGAIDAAVNAYCDANGIAGCSSIWSGFHQYVIINP"
BASE COUNT          728 a         1021 c          924 g          680 t
ORIGIN      
        1 catcggctgc gcgaagcgtt atagttccgg cacgtctcaa agaacaccgc gatgacaagc
       61 ccctcaccgg caccacgcat gcctgttact ataggcgttc acgctcagcc gagttcggcc
      121 ggttcggcgc cggccggccc gctatcgccc aggacgatct tctccagacg gctttccgcg
      181 gccttgagct tgctctcgca atgagccttc aacgcctgcc cgcgttcgta aagcgcgatg
      241 gagtcctcaa gatcgacctg cccctgctcc agccgggcga cgatgccctc aagttccttg
      301 agggcagcct cgaaactgag catgtctaca gaataggctt ccgacttcgc ttttgaggac
      361 atcgcacccg ttcccgtttg tgcgcagaga tgacgatcca cattgtaaaa ggtagcgagg
      421 tcataagcgc ggcggcggct tcagacaata tcgccagccc ttgccgttcg tcgatgtatc
      481 tccgtgatat cacgcgcaac ggaattgccg tggaagcgga acagattgct gagtcatgcg
      541 cattaactac acaaaaaggc tgattaattt ctcatcaaag tgatcatttt atagctttat
      601 aggcaaaatt ggccgcaaca tattgatatt attgagtttt cttttacctt cccaaggagc
      661 aacattcgcc tagataaaca cgaaatataa tggtggttat ttcgtgaagg cattataaat
      721 tgatataata atacattatc acgcaaaatt gacactaaaa tgcaacatgt tctttgcgaa
      781 atagtgcgct gcacaattcg ccttttgacg atccctacca acgcctctag catttttcca
      841 aacgggcaag agacacatac gcagaataca tgcgtgttgt gcggtttttc gataatcggc
      901 cgatgcccgc gagcgaatgt cgttacgaca caaccaaggg ggttttgacg tgtataaatc
      961 cagaaaatcc agtctgttgg cttccacggt cctgcgaggg gggctggcaa ttgcgtttgg
     1021 tcttccactt gctgccctca cagtggcaac accagcggtc gcgcaagact acacgtctgg
     1081 ctcgatcacg ggcacggtgg cagactccgc aggcagcccg gttgcgggag caacggtgac
     1141 gatccggtcc cggggccaag gctttacccg gtcagcttcg accagcgcgt cgggcgggtt
     1201 ttacttctcg ggtcttccgt cgggcgccta tgaagtcacc gtagaagcgg acggctacag
     1261 ggccgagtcc attgcaaatg caagggtgtc ggcctccgct gcgaccacct actcgatcgt
     1321 cctgcgcagc acgacagcgg ttacgggttc cgcggagaca gtggttgtca ccggcacgcg
     1381 ccagaccttc gacttcacca acacgactac cggcgccaca ctgaacattc cggacctcgt
     1441 gaaagacatt ccggtcagcc gcgatctgac ctcaatcatc ctgctcgccc cgggcacggt
     1501 gggcggcgat cgctccttcg gccgcaggtc ggaaggctat ctgccctcga tcggtggttc
     1561 gtcggtcgcc gagaatgcct attacatcaa cggcctgaac atcaccgatt tcaaccacta
     1621 caccggcggc tccctggtgc cgttcgactt ctatgaatcc gtcgaagtca agaccggcgg
     1681 ttatccggct gaattcggcc gcgccaccgg cggcgtggtg aacgccgtta gcaaatcggg
     1741 cagcaacgaa ttcaaggccg cggtccacgt gaactgggaa ccggatgcgc tgcgcgagac
     1801 ggcgcccgat acctataccg cgcgcaacaa gctcgaccac gagagcgcct tcaataccgt
     1861 gctggaactg ggcggcccga tcatcaagga ccacctgttc ttctacggct tgatgcagct
     1921 caaccagaac tcgaccagcg attcgggcat aatctcccaa ctcaccacga aaacatcgac
     1981 caacagtccg ttctggggcg cgaagatcga cggctacatc acggacgacc atcacctcga
     2041 gttcacctat ttcaacacga agcgtatcac gaccctcaaa aactacgctt ttgatgccgc
     2101 caccgatgaa accgctgtgg actcgggcct cgcgcgcgaa gagtggaccg gcggcgaaag
     2161 ctgggtggcg aagtacaccg gccgcatcac ggattggctg acgatctccg cggcctatgg
     2221 cgacaaccgg gaccgcgacg aacagagagg tggacaaggt atcacctaca tcgccgacca
     2281 gaccgttggc ggcttggtct gcggcggaat caccggtgga ttttgtgggt ctcagaatac
     2341 gacggtctat gatattccgc agaccacgaa gcgcaccttc tatcgcgtcg acgtcgacgt
     2401 cctgttcaac ctgctgggcg atcatcacgt ccgcttcggc atggacaacg aatcgaacta
     2461 ccttgcgcac accgccatac gtccgggccc ggaaggctat gccgtcattt accgccagtg
     2521 cgcaacgagg gctgacctgc ctgtggccaa ttggaacgca cgctgcagag ctccagctcc
     2581 tagcactgcg gctttgtatg cgtggaacca gcagttcgtc gaattcaact tctacaacac
     2641 gggcggtgaa ttccgctcgc gcaacaccgc cttttacgtc caggacgagt ggaaggtgct
     2701 ggacaacctg accgtcaacc tcggcgtccg gatcgacggt ttcaacaact tcacggcgga
     2761 cggttcgcaa tacgtggcgt tgcacgggct cttcgccccg cgcgcaggct tctcctactc
     2821 gccgtgggaa gatgacagcg gccgcttcta cggcagcttc ggcgactact acctgccggt
     2881 cgccggcaac acagcgttcc gtcagggcgc gcgtgagttg tacttccagg aatactggtt
     2941 cttcaacggc cttgagcaga acaattatcc gtctctgacg acacaactcg tgggctggcg
     3001 cggggcaaat gcgtgcccgg gtgcgctgac ccccggctcg tccgtgggga cggtcggctg
     3061 cacggtgacc agcgacggca gcgtgcagga tccgacatcc tcgctcgcca agaaccttaa
     3121 ggcgacgaga gagcaggaaa tcatcttcgg ctacgagcag aagctcgacg gactgttgag
     3181 tggattcggc gatctgtgga ccgtcggcat caactacacc cgccgcgatc tcatggtaac
     3241 ggccgaagac ggggctatcg acgccgcggt caacgcctat tgtgacgcga acggcattgc
     3301 gggctgctct tccatctgga gcgggttcca tcagtacgtc atcatcaacc ccg
//
LOCUS       DASWPU010000172         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_44453,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000172 DASWPU010000000
VERSION     DASWPU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44453"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..747)
                     /locus_tag="VGC27_03900"
     CDS             complement(283..747)
                     /locus_tag="VGC27_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0281751.1"
                     /translation="MDRIDINILQALQRDAHVKAADLAESLGISLSPLYRRIRLLEEA
                     GVITKYVSLLDQEKVGFPVTAYVSVAIEKSAAKLAAFERALLGCEEVMECYLMTGAFD
                     YIVRLVASDMAGIERFVMTRLATIEGVRDVSTSITLRRVQYKTVLPVREGGE"
     gene            987..1760
                     /locus_tag="VGC27_03905"
     CDS             987..1760
                     /locus_tag="VGC27_03905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEY0281752.1"
                     /translation="MQLKGANVTFSNGQLAGKVALVTGGSRGIGAAIAKRLASDGAAV
                     SITYSNSRDDADRVIEDIERAGGRGLAIRADCADAEAVKAAVGSTSKRFEGLDILVNN
                     AAMFRRGLIDDFPLEDIDRMLAINVRSLLVAIQESLRFMERGGRIINIGSISSDYMPI
                     PGVSVYAATKGAVTSMTRALARDLGSREITINNVQPGRIDTVMNPANGPMADQIRGSI
                     ALGRYGSGDDIAGLVAWLASPDAAFITGTSLKVDGGTSA"
BASE COUNT          564 a          767 c          772 g          513 t
ORIGIN      
        1 cgcttcttgg ccggtccttg ccggatggcg gtcgtcgctt cccgacccac tcccgcccct
       61 cagcggaccg tcgccaaagt ccgctccgtc gggcgaagcg gacgctgggg gcgctccggt
      121 cggcagcatc ttgtcgaatc gaattggagc agcgtttcaa ttgacgaaag ggcgatctgc
      181 aacctgaagg tcactcagcg cagccaatcg aggtactcaa tgcggcctgg ccccctgagc
      241 agtctgctcc atcgatcggg tactcccggc gcaatcggat atttactctc cgccctcgcg
      301 aaccggtagc acggtcttgt actggacgcg ccgcaaggtg atcgacgtgc tgacgtcgcg
      361 aacaccctcg atggtcgcga gccgcgtcat cacaaaacgc tcgattcccg ccatgtcgct
      421 ggccaccagc cgcacgatgt aatcgaacgc gcccgtcatc agatagcact ccatcacctc
      481 ctcgcaaccc agcagggcgc gctcgaaagc agccagcttt gccgcggatt tttcaattgc
      541 gaccgatacg taagcggtga ccggaaatcc caccttctcc tggtccagaa gtgacacata
      601 ttttgtaatg acgcccgcct cttcgagcag acgaatgcgc cggtagagag gcgaaagcga
      661 aatgccaagc gattcagcga ggtccgcagc cttgacatga gcgtcccgct gtagcgcttg
      721 caagatgtta atgtcgatcc ggtccaattt cattggctca ttgtctcaaa taaacttgaa
      781 aatgtgccta atgttgctac acacggttaa tttattggct cattttggca catacggtcg
      841 gcgcagacta tctaaaattt ctcacacgac atgtcgtgtc ctgtcgcagc agcgcagcgt
      901 ggccgggttc ggcagtgcgc cgctcgcaca tgcttcccgc ttgctgatgt acgaggcaag
      961 cgtggccgat ctgaaacgag cagagaatgc aattgaaagg agcaaacgtg actttttcta
     1021 atggacaact ggctggcaaa gtggcgctcg tgaccggggg ctcacgcggg ataggcgcgg
     1081 ccatcgcaaa acggctcgca agcgatggcg cggccgtgtc gataacctac tcgaactcgc
     1141 gtgacgacgc ggatcgcgtc atcgaggaca tcgaacgcgc cgggggacgg ggtttggcaa
     1201 ttcgcgcgga ttgcgccgac gcggaagcgg taaaagccgc ggtcggatcg acctcgaaac
     1261 gcttcgaggg actggatatt ctcgtgaaca acgccgcgat gttccgccgc ggcctgatcg
     1321 acgacttccc actggaagat atcgaccgga tgctggccat caacgtcagg agcctgcttg
     1381 tcgccattca ggaatcgctc cgcttcatgg aaagaggcgg gaggatcatc aatatcggca
     1441 gcatcagcag cgactacatg ccgattccgg gtgtgtctgt ctacgccgcg acgaaaggtg
     1501 cggtaaccag catgacgcgt gcgctggcaa gggatcttgg cagtcgggaa attacgatca
     1561 ataacgtcca gcccggacga atcgatacgg tgatgaaccc ggccaacggt ccgatggccg
     1621 accagatccg gggctcaatt gcactgggcc ggtacggatc aggcgacgat attgcgggac
     1681 ttgtcgcctg gctggcaagc cccgatgcgg cgttcataac gggcaccagt ctgaaggtcg
     1741 atgggggcac aagtgcttga gcggctcagg tcgaacgtga acggccgttc ggacccgcgc
     1801 catcgcaacg ccgtagatag ccttcaatcg aaccgggtat acgtatgctt ttcgctctcc
     1861 tattagaacg cctcgaccgc tggctgcgga atacccaccg gacccgctgt gatgaatacc
     1921 tcgcagcttc gaaggatatt gccgagcttg agcgtcggat gaaagagtgc gacggttagc
     1981 tcgggccgac gccggtcgcg agcgattcac gataacggcg ccaatgcccc gctctatgcc
     2041 cgccgcgagg gcgactcttc tgaggacaat gcgtcgtcgt tgccatttcc cactttcgag
     2101 gcatggaatt tctgtgacag attgagtggc gggtttggcc aaggttgcga cgtgtatcgt
     2161 cggtgcgcag ctgccggtta ctgatggcga agccgacacc cgaatgtcag gcccacgccc
     2221 caggcgaacg acgcttcatg gcccacagcc cggcttgacg gtagacattc gcagttgccc
     2281 ctcagccacc tcttcatcat cggatatctc actcgagaaa ggaaaaccca gctctgcata
     2341 gggtgggacg accgggtacg gccgcgatct gtgcatggcc gtcgacatga tccgcccttc
     2401 aacgttggcc atggccgtcg cggcagtcaa ttgacgccag agcgggcaag tgtttgtgaa
     2461 tgagtcggcg cagcgacaac tacagataaa tgcaacaata aaatgcagtc agcgcccttt
     2521 gaaactcatc ctgtgacatg gaggcgaaca tgctggaaat cgcgagagat cgggtcatgg
     2581 caacggtgaa cgagattctc gaattccggc tgaccg
//
LOCUS       DASWPU010000173         4797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_68898,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000173 DASWPU010000000
VERSION     DASWPU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68898"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VGC27_03910"
     CDS             <1..197
                     /locus_tag="VGC27_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281753.1"
                     /translation="AVMHRMALGIKASPAIQYVPMSLAADANGNFVCAGEHHHPGERL
                     TFRVTLQDEAAKWYNFQVRS"
     gene            263..1783
                     /locus_tag="VGC27_03915"
     CDS             263..1783
                     /locus_tag="VGC27_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0281754.1"
                     /translation="MQKLFILAAGFVMAAVLIALPASAAMKWSVLTPNVTANVTTHIR
                     VRLVGDAGKPVTRAVNITSVRVDMSPDDMAGMTAPAKLLPSKEPGVVVVETNLYAPGR
                     WAVILSGTVDGKPVKGSVVVTATQKSAEAASPPPAAARRILYYRNPMGLADTSPVPKK
                     DAMGMAYIPVYADEVTKVPGAVRLSTEKIQRAGVRTTAVTRMPLAKTVRATGIVAADE
                     NRQAVLTARFSGFVEKLFVSQTGDVVRAGQPLMRVWIESPEILIKEADYIGSLASHSA
                     EHAAMAANILRQFGVPQSELDAMAKSGVPTRSIMIAAPKGGTVMEKPVVQGMYFAAGD
                     TLFKITDVSHLWVLAEISERDLAAVKPGQKAAITFRDDPGASFEGKVLFVYPELDPAT
                     RTAKVRIAVANKANLLRLGQYADVRIEAPVSTAPVLTVPVSAVIDDGTRQIAFVAGQG
                     GMFEPRKLKLGGRSGDMVEVRAGLKAGERVVTSGNFLIDAESNLQTALQSFTGSSK"
     gene            1780..>4797
                     /locus_tag="VGC27_03920"
     CDS             1780..>4797
                     /locus_tag="VGC27_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY0281755.1"
                     /translation="MIARLIRWSAEHVGVVLTGTIVAILLGVFAVSRIPLDAIPDLSD
                     TQVIVYTDYPGQAPQVVEDQVTFPLTSALLSVPKAKTVRGVSFFGVSFIYVIFDEGTD
                     IYWARSRVLEYLNAAAQQLPQGVTPSLGPDATGVGWVYEYALTGKTQDLAQLRSLQDW
                     HLRYALAKTEGVSEVAGVGGFVKQYAVVADPNRMRAYGITLGQIRSAIESANMDVGGR
                     TIELSETEFMIRGRGYIKSPRDLDRVMLKAKDGTPVLLNAVAHIETVPDERRGLTELN
                     GEGEVASGIVVQRYGANALNVIRAVKARIAEIAASLPAGVEILPVYDRSQLIERAIGT
                     LKSTLLEESIIVALVCFLFLMHARSALVAIITLPVGILIAFVAMNALGIGSNIMSLGG
                     IAIAIGAMVDAAIVMIENAHKHLERAGPDEPRLKVLIAAAVEVGPALFFSLLVITVSF
                     IPIFALQEQEGRLFHPLAFTKSFAMAAAAMLSVTLVPALMALFVRGRIAPEAKNPVNR
                     FLMRVYRPVISAVLKRPWATLGIAAAVLAVSLYPALRLGSEFMPTLNEGTLLYMPTTL
                     PGLSITKAAQILQTQDRIIKSFPEVESVWGKAGRADTATDPAPLEMFETVINLKPQAL
                     WRSGMTTDTLIAALDKALQFPGISNAWTMPIKARTDMLSTGIRTPVGVKVYGKDLDEI
                     EKLSREVEAAVRSVPGTTSAYAERVNGGYYLDITPDRDRIARYGLTIDDVLSTISNAI
                     GAKTITTTVEGRERYGVVLRYPRDVRSDPDAIAADTLVSLPSGGAVPLGEVAKISRAR
                     GPTMVRTEDGQLVNYVYVDFHDRDLGGYVKDAQRAVAGQVEFPPGYYVRWSGQFEYLE
                     RASARLRIVVPATLIIIFGLLWLNFRRISDTLIVMLSLPFALAGGFWLMWAMGFNMSV
                     AAAVGFIALAGVAAETGVVMLIYLNNALAEAKVQAAARGGELTQAELAGAVMKGAVDR
                     VRPKMMTVAAIMAGLLPILWATGTGSEIMQRIAV"
BASE COUNT          831 a         1565 c         1568 g          833 t
ORIGIN      
        1 ttgcggtgat gcaccgtatg gcgctgggca tcaaagcatc tccggccatc cagtatgtgc
       61 cgatgtcgct tgcggcggac gcaaacggca atttcgtgtg tgccggcgaa catcatcatc
      121 ccggggagcg actcaccttc cgcgtcacac ttcaggatga agccgccaag tggtacaact
      181 tccaggtgag aagctgatgc cttggccggg gccggcacag gccggtcccg gcctttccat
      241 aaccgcaatc ccggagattc tcatgcagaa gcttttcatt cttgccgcag gtttcgttat
      301 ggctgcggtt ttgatcgccc ttcccgcctc ggcggcgatg aagtggtctg tcctgacgcc
      361 gaacgtgacc gcgaatgtca ccacgcatat tcgcgtccgg ctcgtcggcg acgccggaaa
      421 acccgtgacg cgcgccgtca acatcacctc cgtccgggtc gatatgagcc ccgacgacat
      481 ggcgggcatg acggcccccg ccaagctgct gccgtcgaag gaacccggcg tggtggtggt
      541 ggagaccaat ctctacgccc cgggccgctg ggcggtgatc ctgtccggaa ccgtggacgg
      601 caagccggtc aagggcagcg tcgttgtaac ggccactcaa aagagcgcgg aggcggcatc
      661 gccgcctccc gccgccgctc gccggattct ctattaccgc aacccgatgg gcttggcgga
      721 tacctcgccg gtgccgaaga aggacgcgat ggggatggcg tatattcccg tctatgccga
      781 cgaggtgacg aaggttcccg gcgccgtccg cctcagcacc gagaaaatcc agcgcgccgg
      841 cgtgcgcacc accgccgtca cccgcatgcc gctcgccaag accgtgcgcg ccaccggcat
      901 cgtcgccgcc gacgagaacc gtcaggcggt tctcaccgcc cgcttcagcg gctttgtcga
      961 aaagctcttc gtctcgcaaa ccggcgacgt tgtgcgggcc gggcagcccc tcatgcgggt
     1021 gtggatcgaa agcccggaga ttctgatcaa ggaagccgat tatatcggct cgctggcgtc
     1081 gcattccgcc gaacacgcgg cgatggccgc gaacatattg cgccaattcg gcgtgccgca
     1141 gtccgaactg gacgccatgg cgaaaagcgg cgtgcccacc cgctcgatca tgatcgccgc
     1201 gccgaagggc ggaacggtga tggaaaagcc ggtggtgcaa ggcatgtatt ttgccgccgg
     1261 cgatacgctg ttcaagatca ccgacgtgtc gcatctgtgg gtgctggcgg aaatatccga
     1321 gcgcgatctg gcggcggtaa aaccgggcca gaaggcggcg atcacgttcc gcgacgatcc
     1381 cggggcctcg ttcgaaggca aggtgctgtt cgtctatccc gaactcgatc ccgcgacccg
     1441 taccgccaag gtgcgcattg cggtggcgaa taaagcaaac cttttgcgcc tggggcagta
     1501 tgccgacgtc aggatcgagg cgccggtctc gacggcgccc gtgctgacgg tgccggtatc
     1561 cgccgtgatc gacgacggca cccgccaaat tgccttcgtg gccggacaag gcggcatgtt
     1621 cgagccgcgc aagctgaagc tgggcgggcg ctccggcgac atggtggagg tgcgggccgg
     1681 gcttaaggcg ggcgaacggg tcgtgacctc cggcaatttc ctgatcgacg ccgagagcaa
     1741 cctgcaaacc gcgctgcaaa gcttcaccgg atcgtccaaa tgatcgctcg cctcatccgt
     1801 tggtcggcgg agcatgtcgg cgtcgtgctg accggcacga ttgtggccat actgctcggc
     1861 gttttcgccg tcagccgcat cccgctcgac gccattcccg atctctccga tacccaggtc
     1921 atcgtctaca ccgattatcc cggccaggcg ccgcaagtgg tggaagacca ggtgacgttc
     1981 ccgctgacct cggcgctcct cagcgtgccc aaggccaaga cggtgcgcgg cgtttccttt
     2041 ttcggcgtct ccttcatcta tgtgatcttc gacgaaggca ccgacatcta ctgggcgcgt
     2101 tcgcgggtgc tggaatatct caacgcggcg gcgcagcaat tgccgcaagg cgtgacgccg
     2161 agcctcggac cggacgccac cggcgtcggc tgggtttacg aatacgccct caccggcaag
     2221 acgcaggacc tggcgcaact gcgctcgctg caagactggc atctgcgcta cgcgctggcc
     2281 aagaccgaag gcgtgtcgga ggtcgccggc gtcggcggtt tcgtcaagca atatgcggtg
     2341 gtggcggacc ccaaccggat gcgggcttac ggcatcaccc tgggacaaat ccgcagcgcg
     2401 atagagagcg ccaatatgga cgtcggcggg cgcaccatcg agctgtccga aaccgagttc
     2461 atgatccgcg gccgcggcta catcaaatcc ccgcgcgacc tcgaccgggt gatgctgaag
     2521 gccaaggacg gcacgccggt cctgctcaac gccgtggccc atatcgaaac cgtgccggac
     2581 gagcgacgcg gcctcaccga gctgaacggc gaaggggaag tggcgtccgg catcgtggtg
     2641 cagcgctatg gcgccaatgc gcttaacgtc atccgggcgg tgaaggcgcg gatcgccgag
     2701 atcgccgcca gccttccggc cggggtcgaa atcctgcccg tctacgaccg ttcgcaattg
     2761 atcgagcgcg ccatcggcac gctgaagagc acgctgctgg aggaaagcat catcgtggcg
     2821 ctggtctgct tcctgtttct gatgcatgcg cgcagcgccc tggtcgccat catcaccctg
     2881 ccggtcggca ttctgatcgc gttcgtcgcc atgaacgcgc tgggcatcgg ctccaacatc
     2941 atgagcctgg gcggcatcgc catcgccatc ggcgccatgg tggacgcggc catcgtgatg
     3001 atcgagaacg cccacaagca cctggagcgc gcgggacctg acgagccgcg cctgaaagtg
     3061 ctgatcgcgg cggcggtgga agtcggcccg gcgctgttct tcagcctctt ggtcatcacc
     3121 gtttcgttca ttccgatctt cgcgctgcaa gagcaggagg gccggctgtt ccatccgctc
     3181 gccttcacca agagcttcgc gatggcggcg gcggcgatgc tgtccgtgac cctggtgccc
     3241 gcgctgatgg cgctgttcgt gcgcggccgg atcgccccgg aagcgaagaa cccggtgaac
     3301 cgtttcctga tgcgggtcta tcgcccggtg atcagcgcgg tgctgaagcg gccttgggcc
     3361 acgctcggca tcgcggcggc ggtactcgcg gtcagccttt atcccgcttt gcgcctcggc
     3421 agcgagttca tgccgacgct caacgaaggc acgctgctct acatgccgac gaccttgccc
     3481 ggcctgtcga tcaccaaggc ggcgcagatc ctgcaaaccc aggaccgcat catcaagagc
     3541 ttcccggagg tggaatcggt atggggcaag gcgggccgcg ccgacaccgc caccgatccc
     3601 gcgccgctgg agatgttcga gaccgtcatc aacctcaagc cgcaagcgct atggcgttcg
     3661 ggcatgacca ccgacacgct gattgcggcg ctcgacaagg cgctgcaatt ccccggcatc
     3721 agcaacgcct ggaccatgcc gatcaaggcg cgcaccgaca tgctgtccac cggcatccgc
     3781 accccggtcg gggtcaaggt ctacggcaag gacctcgacg agatcgagaa gctgtcgcgc
     3841 gaagtggagg ccgcggtgcg cagcgttccc ggcaccacct ccgcctatgc cgagcgcgtc
     3901 aacggcggct attacctcga catcaccccc gaccgcgacc ggatcgcgcg ctacggcctc
     3961 acgatagacg acgttctctc cacgatttcg aacgccatcg gcgccaagac gatcaccacc
     4021 acggtggaag ggcgggagcg ctacggcgtg gtgctgcgct atccgcgcga cgtgcgcagc
     4081 gatcccgacg ccatcgcggc ggacacgttg gtctcgctgc cgtcgggcgg ggcggtcccg
     4141 ctgggcgagg tggcgaagat ttcgcgcgcc cggggaccga cgatggtccg caccgaagac
     4201 gggcagctgg tgaattacgt ctatgtggat ttccacgacc gcgacctcgg cggttacgtg
     4261 aaggatgccc agcgcgcggt ggccgggcag gtggaattcc cgcccggcta ttacgtgcgc
     4321 tggagcggcc agttcgaata tctggagcgc gccagcgcgc ggctccgcat cgtggtgccc
     4381 gccaccttga tcatcatctt cggactgtta tggctcaact tccggcggat ttcggacacg
     4441 ctgatcgtca tgctgtcctt gcccttcgcc ttggccggcg gcttctggct gatgtgggcg
     4501 atgggcttca acatgtcggt ggcggcggcg gtgggcttca tcgcgctggc gggcgtggcg
     4561 gcggagaccg gggtggtgat gctgatctat ctgaacaacg cgctggcgga agcgaaagtc
     4621 caagccgccg ccagaggcgg agaactgacc caggcggagc ttgcgggtgc ggtgatgaag
     4681 ggcgcggtgg accgcgtgcg tcccaagatg atgaccgtcg ccgcgatcat ggcgggcctc
     4741 ttgccgattc tatgggcgac cggcaccggc tcggagatca tgcagcgcat cgccgtg
//
LOCUS       DASWPU010000174         3930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_7542, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000174 DASWPU010000000
VERSION     DASWPU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7542"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VGC27_03925"
     CDS             <1..474
                     /locus_tag="VGC27_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0281756.1"
                     /translation="PADLSGMDLRAVPALGSRPLTALIAPEAIFYGLNLEGASLQGSN
                     LQGADLRSVRLVGADLRGVNLVGAKLNYADLRDAKLGPLLISDNRLLPARLDNVTARY
                     ADFRGADLRRASLTGGALAYANLIDANLRDTDTEQADLTGAKLPAMFVESAAAAG"
     gene            complement(475..1407)
                     /locus_tag="VGC27_03930"
     CDS             complement(475..1407)
                     /locus_tag="VGC27_03930"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0281757.1"
                     /translation="MPSWQSAFFTRFSRMAKPQAADASTDIAELRRQYAYLSDRFGAV
                     PGDAVFEPASLGPVKGEWVRTETSSPQRLILYFHGGGYIAGSPETHRALVAKLAYAAE
                     ASAFSVAYRLAPECAFPAAVRDGIDAYRQLLGRGLAPQAIVLGGNGSGGGLAFSVLLA
                     IRNAGLPMPAACVAMSPWADLSLSGWSVLQNAKTDTAQSWELLFVSARHYLKRANPSD
                     PYASPVFASFKDFPPIMVHAGSLDMLRDDASWLGDRAADAGVPVSVEIYDGMQHVFQA
                     NPVPEARVSLQRLGQFIRARIPIAREDEPVAAHR"
     gene            complement(1531..1986)
                     /locus_tag="VGC27_03935"
     CDS             complement(1531..1986)
                     /locus_tag="VGC27_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY0281758.1"
                     /translation="MRAFDFSPLFRSTVGFDRLFDLIDSYAEQSQGYPPYNIERSDET
                     HYRITLAVAGFAEKDLSAEVREGVLSVVGKRDEAEASDNKGYLYQGIAGRAFERRFQL
                     AEHVEVREARLENGLLHIDLERVIPEEKKARQIPIGAPGMKTIEGRKAA"
     gene            complement(2204..2620)
                     /locus_tag="VGC27_03940"
     CDS             complement(2204..2620)
                     /locus_tag="VGC27_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281759.1"
                     /translation="MAIAPDKETADTAAQAPPRDLLGKVCFSLHVAILIVILAGWAIP
                     ARAALIFYLVLLPGVVLHWRLNANSCVLNNLESWIRNGRWRDPDNREEGQWLRTLIKD
                     VTGLEVSRTQMDVLVYGVYGTLAVLWGVGLWHLLGW"
     gene            2764..3087
                     /locus_tag="VGC27_03945"
     CDS             2764..3087
                     /locus_tag="VGC27_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY0281760.1"
                     /translation="MSTPLVEKIITEMGSRLGDNSGLGGTLKFDFGEPGSVYIDGKSV
                     PNTVGDGAGKNADCTITVSLETFEKMVKGELDGTSAFMQGKLRVAGDMGLAMKLGPVL
                     QKARG"
     gene            3197..>3930
                     /locus_tag="VGC27_03950"
     CDS             3197..>3930
                     /locus_tag="VGC27_03950"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0281761.1"
                     /translation="MLPFSPRFITTRDGTRLRTAVFAAESSRGVCVLLGGQTEFIEKY
                     AEVIGELGARGFTVATFDWRGQGGSARALANPLMAHVGDFAEYDDDLTSFLEQVVEPL
                     SPKPLALAHSMGGQILLRALHDRPDAFSAAIFSAPMLGISTRGHPAPLVRLATALQNL
                     AGRSGTFAWGMAEHDPFAITFDRQLCTSDRARFARTQEILKNNPSLRLAGPTWGWIGA
                     AYRSMARVTAPGFAQAIQVPVLIVGAG"
BASE COUNT          680 a         1292 c         1274 g          684 t
ORIGIN      
        1 cccgccgatc tctccggcat ggatttgcgg gccgtaccgg cgctcggcag ccggccgctg
       61 accgctttga tcgcgccgga ggcgattttc tacggcctca atctcgaagg cgcctccctg
      121 caaggcagca acctgcaagg cgccgacctg cgctcggtcc ggctggtggg cgcggatttg
      181 cgcggggtca atctggtcgg cgccaagctg aactacgccg accttcgcga cgccaagctc
      241 ggaccgctgc tgatttccga caaccggctg ctgcccgcgc ggctcgacaa tgtgacggcg
      301 cgctatgccg attttcgcgg cgccgacctg agaagggcca gtctgaccgg cggcgccctg
      361 gcctacgcca atctcatcga cgccaatctg cgcgacaccg acaccgagca agccgatctc
      421 accggcgcca agctgcccgc gatgttcgtg gagagcgccg ccgcggcggg ctgactatct
      481 gtgcgccgca accggctcgt cttcgcgcgc gatggggatg cgggcgcgga tgaactggcc
      541 cagccgctgt aggcttaccc tcgcttcggg caccggattc gcctggaaga cgtgctgcat
      601 gccgtcgtag atttcaacgc tgacgggcac gcccgcgtcc gcggcgcggt cgccgagcca
      661 cgaggcgtcg tcgcgcagca tatccagact gccggcatgg accatgatgg gcggaaaatc
      721 cttgaagctg gcgaacaccg gcgaggcgta gggatcggaa gggttggcgc gcttcagata
      781 atggcgcgcg ctgacgaaca gaagttccca ggactgcgcg gtgtcggttt tggcgttctg
      841 cagcacgctc cagcccgaca gcgacaggtc cgcccagggc gacatcgcca cgcaggccgc
      901 cggcatcggc aggccggcat tgcggatcgc cagcagcacc gaaaaagcca gcccgccgcc
      961 cgagccgttg ccgcccagca cgatcgcttg cggcgccagc ccccgcccca gcaattgccg
     1021 gtaggcgtcg atgccgtcgc gcacggcggc cgggaaggcg cattccggcg ccagccggta
     1081 ggcgacggaa aaagcgctgg cctcggcggc gtaggcgagc ttggcaacca gggcgcgatg
     1141 ggtttcgggc gagccggcga tgtagccccc gccgtggaaa taaagaatca gccgctgcgg
     1201 cgaagacgtc tccgtgcgca cccattcgcc tttgaccggg cccagcgacg ccggctcgaa
     1261 cacggcgtcg cccggcacgg cgccgaaacg gtccgacaga taggcgtatt gccggcgcag
     1321 ttccgcgata tcggtgctgg cgtcggccgc ctgcggcttc gccatgcgcg agaagcgggt
     1381 gaaaaaagcg ctttgccagc ttggcatttt ggggccgcac ctccgaaatc cgcgctctga
     1441 atagcagata acagcatcgc ccgtcgaaaa agaaaagccg ggagcgcagt tccgctcccg
     1501 gcctttttgg ctgttgccgt tatcttccgc tcaggcggct ttacggccct cgatggtctt
     1561 catgccgggc gcgccgatcg gaatctggcg ggccttcttc tcttcgggga tcacccgctc
     1621 cagatctatg tggagcagcc cgttttcgag cctggcctcg cgcacttcca catgctcggc
     1681 gagctggaag cggcgctcga aggcgcggcc cgcgatgccc tgatagagat agcccttgtt
     1741 atcggacgcc tcggcttcgt cgcgcttgcc gacgaccgag agcacgcctt cgcgcacttc
     1801 cgcgctcaaa tccttttcgg cgaagccggc caccgccagg gtgatgcggt aatgggtctc
     1861 gtcggagcgc tcgatattgt agggcggata gccttggctt tgctcggcat agctgtcgat
     1921 caggtcgaac agacggtcga agccgacggt ggagcggaac aggggagaaa agtcgaatgc
     1981 acgcatgatc aaacctcctt gaagcgtttg gttgtggccc tgccttgcgg ccaggaccgt
     2041 tttccttgcc gggagccttc aggcctcccg gcacctcttt agatggggag caccaaaacc
     2101 ggcttcaagg ggttttctgc ccccgccttg cccaccgaag ctttagcgta ggtggggagc
     2161 gggggaagca tctgtgttgc atgtcaaggg gcgaaagggg cattcaccac cccagcaaat
     2221 gccacaaccc caccccccac agcaccgcca gcgtgccata gacgccatag actaagacgt
     2281 ccatctgggt gcggctgact tccaggccgg tgacgtcctt gatcagggtg cgcagccatt
     2341 ggccttcttc ccggttgtcc ggatcgcgcc agcgcccgtt tctgatccac gattccagat
     2401 tgttgaggac gcaggaattc gcgttgagcc gccaatgcag gaccacgccg ggcagcaaga
     2461 cgagatagaa gatcagcgcc gcgcgcgccg ggatcgccca gccggcgaga atgacgatca
     2521 ggatcgcgac atgcagagag aagcagacct tgcccaagag atcgcgcggc ggtgcctgcg
     2581 cggcggtgtc ggccgtttcc ttgtcgggcg cgattgccat gcgttttaat tccctgcacc
     2641 gcggtcaagc ttgcgcttga agcgtgagca tgcctgttgt caccaagact gacaaatgct
     2701 caattaagct tgtttcgcgg cccggtcgcg gccgggccgc aactcttagc gggaaggatg
     2761 tccatgagca cgccactggt tgaaaagatc atcaccgaga tgggcagccg tctgggagac
     2821 aattcggggc ttggcggcac gctcaaattc gatttcggcg agccgggcag cgtttatatc
     2881 gacggcaaat ccgtgcccaa cacggtcggc gacggcgccg gcaagaacgc cgactgcacg
     2941 atcacggtca gcctcgaaac cttcgagaag atggtcaagg gcgagcttga cggcacttcc
     3001 gccttcatgc agggcaagct gcgcgtggcc ggcgacatgg ggctggcgat gaagctcggc
     3061 cccgtcctgc aaaaggcccg cggttagctt tctgcccccg ccccgcccac cgaagcttta
     3121 gcgtaggtgg ggagcggggg aagtggcgag ccggtcatta gcgtcagcgt aatgacgcgg
     3181 cgagccgatg ggggccatgc tgccattttc accccgcttc atcaccacgc gcgacggcac
     3241 ccgcctgcgc accgccgtct tcgcggcgga gtcttcgcgc ggcgtttgcg ttctgctcgg
     3301 cggccagacc gagttcatcg aaaaatatgc cgaagtgatc ggcgagcttg gcgcccgcgg
     3361 cttcacggtt gcgactttcg actggcgcgg ccaaggcggc agcgcgcgtg cattggcaaa
     3421 tccgctcatg gcccatgtgg gcgacttcgc cgaatatgac gacgacctga ccagcttcct
     3481 cgaacaggtg gtcgaacctt tgtcgccgaa gccgctggcg ctggcccact ccatgggcgg
     3541 acagattctt ctgcgtgcgc tgcacgacag gccggacgcg ttctcggccg ccattttcag
     3601 cgcgccgatg ctgggcattt ccacacgcgg tcatcccgcg ccgctggtgc gcctcgccac
     3661 ggcgctgcag aacctcgccg gccgctccgg cacctttgcc tggggcatgg ccgaacacga
     3721 tccattcgcc attacgttcg atcggcagct ttgcacctcc gaccgcgccc gcttcgcgcg
     3781 cacgcaggaa attctgaaaa acaatccatc gctccgcctg gcggggccga cctggggctg
     3841 gatcggagcc gcctaccgct ccatggcgcg ggtgacggcg ccgggcttcg cgcaggccat
     3901 acaggtgccg gtgctcatcg tcggcgcggg
//
LOCUS       DASWPU010000175         8069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_117707,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000175 DASWPU010000000
VERSION     DASWPU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117707"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /gene="ppk1"
                     /locus_tag="VGC27_03955"
     CDS             <1..1021
                     /gene="ppk1"
                     /locus_tag="VGC27_03955"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY0281762.1"
                     /translation="YRTSDDSPIVSALIEAADSGKSVTALVELKARFDEAANIKWARD
                     LERAGVQVVYGFIELKTHAKVSLVVRREGGQLRTYVHYGTGNYHPVTAKIYTDLSLFS
                     ADPALGRDAAQLFNYVTGYAEPMGLEKIAVSPLDLRDTLKRAIDEEIAHARAGRPAAI
                     WVKVNSLVDSKMIDRLYRASQAGVRIELVVRGICCLRPGVPGLSDNIRVKSIVGRFLE
                     HSRIVCFGNGYGLPSAKAKVYISSADWMPRNLDRRVEALVPIENPTVHQQVLDQIMVA
                     LLKDQAQSWTMEGDGTYTRDPAADSADAFSVHTYFMTNPSLSGRGRSLRIDKPRPVAI
                     VSRRD"
     gene            complement(1099..1269)
                     /locus_tag="VGC27_03960"
     CDS             complement(1099..1269)
                     /locus_tag="VGC27_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281763.1"
                     /translation="MAATLPILPRSGRSRAQRDAAHRASAATQRAGKSGASRRVSAKI
                     ALWRVESLDRIT"
     gene            1268..2827
                     /locus_tag="VGC27_03965"
     CDS             1268..2827
                     /locus_tag="VGC27_03965"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEY0281764.1"
                     /translation="MTIIESGSDMSGIPILPADLDATMPVPSARNAMARIAVVDIGSN
                     SVRLVVYESLSRSAPTVHNEKSICSIGRDMVSTGLLHKEGCELALESLRRFRVLAEKL
                     GVAVREAVATAAARDAANGPEFVRRAEAAWGAPVRVLSGKEEARFAGQGVIAAIPDAD
                     GLAADLGGGSLDMTTVKNGRTGDACSLPFGPLRLIDLAKGDTDKARNLVDGELAKLPQ
                     LRTLDNRTLYAVGGIWRSLARVDMLREHYPLHMLQHYVVPADRALDLCNVLAKQSRKS
                     LELMSIVSKRRMELLPYGAVVLHRLLTAAKFKEIVVCANGLREGLLYDRLPDAERAKD
                     PLIDFAAAENARLSRAPAHALEMFHWASPLFADENAESRRLRLAASLLSDIGWRRHPD
                     YRARGTYEEVLYMPFAGADHRAREFIATSVFHRYSGDQDIPDGLVVPGWLNKNDGERA
                     LRIGMAARVAFDLSASAPGELPNYRLRLTPTKVILEVPKRRSVIADDTVSRRLASLAS
                     TMDRKAEFLVG"
     gene            2917..3150
                     /locus_tag="VGC27_03970"
     CDS             2917..3150
                     /locus_tag="VGC27_03970"
                     /inference="COORDINATES: protein motif:HMM:NF001940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY0281765.1"
                     /translation="MGGLSFWHILLLAVIVMVLFGGRGKISEFMGDFAKGIKSFKKGL
                     SDEEEAPPPPPRTIDTDLRDETKTPNKDTSARS"
     gene            3226..3627
                     /gene="tatB"
                     /locus_tag="VGC27_03975"
     CDS             3226..3627
                     /gene="tatB"
                     /locus_tag="VGC27_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY0281766.1"
                     /translation="MFPDLSWSHMLLVLVVALVVVGPKDLPKLMHAIGRWASKARAMA
                     DQFRKSFDEMARQSELDELRKELDELRNMRALAETERTVTEALQSPDISLAEAKPEPA
                     DPSEIAAVEAEEPQSAEPSEPIGEGHEPRAP"
     gene            3624..4436
                     /gene="tatC"
                     /locus_tag="VGC27_03980"
     CDS             3624..4436
                     /gene="tatC"
                     /locus_tag="VGC27_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY0281767.1"
                     /translation="MTTTPDDEAEIESTKAPLLEHLVELRKRIIYSLLAFAAAFVICF
                     SFAKPIYAFLTEPLAQALIGQPNRHLIYTALYETFFTYMKVGMFAGMCLAFPVIAAQI
                     WLFVAPGLYRKERKAFLPFLIASPVLFIAGASFVFYVMLPFAIRFFLGFETQGGEGTL
                     GIELMAKVSDYLDFVMTLIFAFGLTFQMPVLLTLLGRVGIVTSAMLRKARRYSIVGIA
                     ALAAVITPPDLFSMVSLAVPLVLLYEVSIWCVGLIERRRAAEDTAREAETPS"
     gene            4518..5837
                     /gene="serS"
                     /locus_tag="VGC27_03985"
     CDS             4518..5837
                     /gene="serS"
                     /locus_tag="VGC27_03985"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY0281768.1"
                     /translation="MHDIKQIRENRDGFVAGLARRAAQFDGKSPDETVAAVLDLDSRW
                     RAATASFERHRARQNEASRAIGQAKAKKDEAGAAALLAEVAGLKEAIQKDEAEQRTLE
                     EELKKALSVLPNLPADDIPDGPDEAFNVEVAARKFGAPPAMNFKPKEHYDLGEALGLM
                     DFERAAKVSGARFVYLKGALARMERALAAFMLDLHTGTFGYTEVNPPFMVRDDAFFGT
                     GQLPKFEDDLFWTPSGEGSRFGLIPTAEVPLTNYVREELLDEAQLPLRFTAHTPCFRA
                     EAGAAGRDTRGMIRQHQFSKVELVSITTPEQSAAEHERMTDCAQEVLKRLGLAHRVMT
                     LCSGDMGFSARKTYDIEVWLPGQDRFREISSCSNCGDFQARRMNTRYRPKEGKATRFV
                     HTLNGSGLAVGRTLVAVLENYQESDGSIRIPDALQPYMGGLKKIEKK"
     gene            5996..6817
                     /gene="surE"
                     /locus_tag="VGC27_03990"
     CDS             5996..6817
                     /gene="surE"
                     /locus_tag="VGC27_03990"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY0281769.1"
                     /translation="MSDKLRILLTNDDGIHAKGLEVAETIARALTDDVWVVAPETEQS
                     GSSHSLTLVRPLRLRAIDSRHFAVTGTPTDCVMMATTHLMKDSQPTLVLSGVNRGVNA
                     ADDVTYSGTIAGAMEGCALDIPSLALSQAYNFEEKGEVQWACAETHGPDLIRKLIEIG
                     WPQDVLININFPDCPPDAVKGVEIVAQGKRDLQEAVIDRRVDMRGYPYFWIGFRRERV
                     SAKPGTDLGALYAKKISITPLQLNLTETAVMERLKQELDFTLPREGRTAKPFEEG"
     gene            6951..7607
                     /locus_tag="VGC27_03995"
     CDS             6951..7607
                     /locus_tag="VGC27_03995"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY0281770.1"
                     /translation="MALRKQGITDTRVLSAIERCPREAFVDAPLAHSAYDNTALPIAC
                     GQTISQPYVVAYATEALEVGPAMRVLEIGTGSGYQAAVLSPLCRKVYTIERHKPLLRE
                     AEARFKALKLENIVTKHGDGLNGWPEQAPFDRILLSAAVPEVPQILIEQLKPGGILVA
                     PVGPATISSMESFSQHLTKMIRSETGLKREALIPVVFVPMMPGVPQEPRTTHGRSDTA
                     "
     gene            7585..>8069
                     /locus_tag="VGC27_04000"
     CDS             7585..>8069
                     /locus_tag="VGC27_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281771.1"
                     /translation="MDVRIPHDVWRGLILALIVPAFLAGCTSTPKTSYDWGVNTRLHH
                     RTRVAQQTHAAPQKTYSRSDTSSYSNTANPRVVPKPRPEPGWYQDSARRPPRVAENNT
                     PAPVQTNIQFAWPVKGQVLSNFGSTTGGERNDGINIAASYGEPIHAAADGTVSYSGNA
                     L"
BASE COUNT         1459 a         2681 c         2555 g         1374 t
ORIGIN      
        1 gtaccgcacc agcgacgatt cgccgatcgt cagcgcactg atcgaggccg ccgattcggg
       61 caagtccgtc accgcgctgg tcgagctgaa ggcgcgcttc gacgaggccg ccaacatcaa
      121 atgggcgcgc gacctggaac gcgcgggagt tcaggtcgtg tacggcttca tcgagcttaa
      181 gacccacgcc aaagtcagtc tggtggtgcg ccgcgagggc ggacaattgc gcacctatgt
      241 gcattacggc accggcaatt atcacccggt cacggccaag atttacacgg atctgtcatt
      301 attcagcgcc gatccggcgc tgggacgcga tgccgcacag cttttcaact acgtgacggg
      361 ctatgccgaa cccatgggcc tggaaaagat cgcggtttcg ccgctcgacc tgcgcgatac
      421 gctgaagcgc gcaatcgatg aggaaatcgc ccacgcgcgc gccggccgtc cggccgcgat
      481 ctgggtgaag gtcaactctc tggtcgattc gaaaatgata gaccggctct accgcgccag
      541 ccaggcgggc gtgcgcatcg agctggtggt gcggggcatc tgctgcctca ggcccggcgt
      601 gcccggcctc tcggacaata tccgcgtcaa gagcatcgtc ggccggttcc tggagcacag
      661 ccgcatcgtc tgtttcggca acggctacgg cctgccgtcg gcgaaggcga aggtgtacat
      721 ctcctcggcg gattggatgc cgcgcaatct cgaccgccgc gtcgaagccc tggtgccgat
      781 cgagaacccg accgtccatc agcaggtgct ggaccagatc atggtggccc tgctcaagga
      841 tcaggcgcag agctggacca tggagggcga cggcacctac acgcgcgatc cggcggcgga
      901 cagcgcggac gccttttcgg tgcataccta tttcatgacc aatccgtcgc tgtccggacg
      961 cgggcggtcg ctgcgcatcg acaagccgcg ccccgtcgcc atcgtcagcc gccgcgattg
     1021 agagcccgcc tcgataatcc tcgtgagccg cgtttcgtgc gaaaaccgcc ggatggaagg
     1081 agaaagccga tgagcgtatc aggtaatacg gtcgaggctt tcgacgcgcc agagggcgat
     1141 tttcgccgaa acccttcggg acgcgccgct tttgcccgcc cgctgcgtcg ccgcgcttgc
     1201 ccgatgtgcc gcatcgcgct gcgcgcggct cctgccggat cggggcaaaa tcggcagcgt
     1261 cgcggccatg acgattatcg agtcgggctc tgacatgagc ggcatcccga tcttgcccgc
     1321 ggacctcgat gcgacgatgc cggtgccttc ggcgcggaac gcgatggcgc ggatcgcggt
     1381 cgtcgatatc ggttcgaatt ccgtccggtt ggtcgtgtac gagtcgctgt cgcgcagcgc
     1441 tccgaccgtg cataacgaga aatcgatctg cagcatcggc cgcgacatgg tgagcaccgg
     1501 ccttctccac aaggagggct gcgaactggc gctcgagtcg ctgcgccgct tccgggttct
     1561 ggccgaaaag ctcggcgtcg ccgttcgcga ggcggtggcg accgcggcgg cgcgcgacgc
     1621 cgccaacggc ccggaattcg tgcgccgcgc ggaagcggcc tggggcgcgc ccgtgcgcgt
     1681 gctttccggc aaggaggaag cccggttcgc cggccaaggc gtgatcgccg ccattcccga
     1741 cgccgacggc ctcgccgccg atctcggcgg cggcagcctc gacatgacga cggtgaaaaa
     1801 cggccgcacc ggcgatgcct gttcgctgcc tttcggtccg ctccggctga tcgatctggc
     1861 caagggcgac accgacaagg cccgcaatct ggtggacggc gaactggcga agctgccgca
     1921 gctcagaacg ctcgataatc gcacgctcta cgcggtcggg ggcatctggc gcagcttggc
     1981 gcgcgtcgac atgctgcggg aacattaccc gttgcacatg ctgcaacatt acgtcgttcc
     2041 ggccgaccgt gcgctggacc tctgcaacgt gctggccaag cagagccgca aatcgctcga
     2101 actgatgtcg atcgtgtcca agcggcgcat ggagcttctg ccctatgggg cggtcgtcct
     2161 gcatcgcctg ctgacggccg cgaagttcaa ggaaatcgtg gtctgcgcca acgggctgcg
     2221 cgagggcctg ctctacgaca ggctgccgga tgcggagcgc gccaaggatc cgctgatcga
     2281 ttttgccgcc gccgagaacg cgcggttgag ccgcgcgccg gcccacgcgc tggagatgtt
     2341 ccactgggcc tctcccctgt tcgccgacga gaatgcggag tcccgccgtc tgcgcctggc
     2401 ggcaagcctg ctcagcgaca tcggctggcg gcggcatccc gattaccgcg cgcgcggcac
     2461 ctatgaagag gtgctttaca tgccgttcgc cggcgccgat caccgagcgc gggaattcat
     2521 cgccacctcc gtttttcacc gctattccgg cgaccaggat atccccgacg gtctggttgt
     2581 ccccggctgg ctcaacaaga atgacggcga acgggcgttg cgtatcggca tggcggcgag
     2641 ggtggcgttc gacctctcgg cctcggcccc cggagagttg ccgaactacc gcctgcggct
     2701 gacccccacc aaggtcattc tcgaagtgcc caagcgccgc agcgtgatcg ccgacgacac
     2761 ggtgagcagg cggctggctt cgctcgcctc gacgatggac cgcaaggcgg aattcctggt
     2821 cggttaatcg tgcgacttgc gttcgagggg gtgatcgggc tttaacaagg accaccggct
     2881 gcggcacaac ccggccgggc attaaggaga tacgtcatgg gcggactgag cttctggcac
     2941 atcctgttgc tggctgtgat cgtgatggtt cttttcggcg gccgcggaaa gatttccgag
     3001 ttcatgggcg acttcgccaa gggcatcaag agcttcaaga agggcctgtc cgacgaggaa
     3061 gaggctccgc cgcctccgcc gcgcacgatc gataccgatc tgcgcgacga aaccaagacg
     3121 ccgaacaagg acacgtccgc gcgctcctaa gaggcgttgt ccttgtgccg agccgcctca
     3181 tcccgagctt gccgcgggac gaaacataag ggtatccggt agcgcatgtt ccccgatctc
     3241 agttggagcc atatgctcct cgttttggtg gtcgcgcttg tggtcgtggg acccaaggac
     3301 ttgccgaaac tgatgcacgc catcggccgc tgggcgagca aggcgcgcgc catggccgat
     3361 cagttccgca agagcttcga cgagatggcg cgccagagcg agctggacga actgcgcaag
     3421 gaactcgacg aactgcgcaa catgcgtgcg ctggccgaga ccgaacgcac ggtgaccgag
     3481 gcgctgcaat cgcccgatat ttccctcgcg gaggccaagc ccgagcccgc cgatccgtcc
     3541 gagattgcgg ccgtcgaggc ggaggagccc cagtccgccg aaccgtccga gccgatcggc
     3601 gaaggccacg agccgcgcgc gccatgacga caacgcccga cgacgaagcg gaaatcgaat
     3661 ccaccaaggc gccgctgctc gaacatctgg tcgagctgag aaagcggatc atctattcgc
     3721 tgctcgcctt cgccgccgcg ttcgtgatct gcttctcctt cgccaagccg atctacgcct
     3781 tcctgaccga acccttggcc caggcgctga tcggccagcc caaccggcac ctgatctaca
     3841 ccgcgctgta cgagaccttc ttcacctaca tgaaggtcgg catgttcgcg gggatgtgcc
     3901 tggcctttcc cgtcattgcc gcgcagattt ggctgttcgt ggcgcccggc ctctaccgca
     3961 aagagcgcaa agcctttctg ccgttcctga tcgccagccc ggtgctgttc atcgccggcg
     4021 ccagcttcgt cttctatgtg atgctgccgt tcgccatccg cttcttcctc ggcttcgaga
     4081 cccagggcgg cgagggcacc ctcggcatcg agttgatggc caaggtcagc gactatctcg
     4141 atttcgtcat gacgctgatc ttcgccttcg gcctgacctt tcagatgccg gtgctgctga
     4201 cgctgctcgg gcgcgtcggc atcgtcacgt ccgccatgct gcgcaaggcg cggcgttatt
     4261 cgatcgtcgg cattgcggcg ctcgccgccg tcatcacccc gccggacctg ttcagcatgg
     4321 tcagcctggc ggtgccgctg gtgctgctct acgaggtctc gatctggtgc gtcggcctga
     4381 tcgagcggcg ccgcgccgcg gaagacaccg caagagaggc ggagacgccc tcataagcgc
     4441 ctgagaacgc tgtcacaatt acaaacttta ccggccggac ggcccgtgcc attacctgcc
     4501 cggccggaga gattcccatg cacgacatca agcaaattcg cgaaaaccgg gacggtttcg
     4561 tcgccgggct ggcgcggcgc gccgcccaat tcgacggcaa gtcgccggac gagacggttg
     4621 ccgccgtcct ggacctcgac agtcgctggc gcgccgccac ggcgtcgttc gagcggcacc
     4681 gggcgcgcca gaacgaagcc tcgcgcgcca tcggccaggc caaggccaag aaggacgaag
     4741 cgggcgccgc cgcgctgctg gccgaagtcg ccggcctcaa ggaagcgatc cagaaggacg
     4801 aggcagagca gcgcacgctt gaggaagaac tgaagaaagc gctgtcggtt ctgcccaacc
     4861 tgccggcgga cgatattccc gacggccccg acgaagcctt caacgtggaa gtcgcggcgc
     4921 ggaagttcgg cgccccgccg gcgatgaact tcaagcccaa ggagcattac gatctcggcg
     4981 aggcgctcgg cctgatggat ttcgaacggg ccgcgaaagt ctccggggcg cggttcgttt
     5041 atctgaaggg cgcactggcg cggatggagc gcgcgctggc ggctttcatg ctcgacctgc
     5101 acaccgggac gttcggctac accgaagtca acccgccgtt catggtgcgc gacgatgctt
     5161 tcttcggcac cggccaattg ccgaagtttg aagacgattt gttttggact ccaagcggcg
     5221 agggcagccg tttcggcctc attcccaccg ccgaagtgcc gctgacgaat tacgtgcggg
     5281 aggaactgct cgacgaggcg caactgccgc tgcgcttcac cgcgcatacg ccctgcttcc
     5341 gcgccgaggc gggcgccgcc gggcgcgaca cgcgcggcat gatccgccag catcagttct
     5401 ccaaggtcga gctggtttcg atcaccacgc ccgaacaatc cgccgccgag cacgagcgca
     5461 tgaccgattg cgcacaagaa gtgctcaagc ggctcggcct cgcccatcgc gtgatgacgc
     5521 tgtgcagcgg cgacatgggc ttttcggcgc ggaagaccta tgacatcgaa gtctggctgc
     5581 ccggccagga ccgcttccgc gaaatctcct cctgctccaa ctgcggcgat ttccaggcgc
     5641 ggcgcatgaa cacgcgctac cgtccgaagg aaggcaaagc cacgcgcttc gtccatacgc
     5701 tgaacggctc cggtctcgcg gtcggccgca ccctggtcgc cgttctggag aattatcagg
     5761 aaagcgacgg ctccattcgc attcccgacg cgttgcagcc ctatatgggc ggattaaaga
     5821 agatcgagaa gaaataaaga atgaggccgc gaacgacacc atccatcggc aagcgtattt
     5881 gcacgcggag gcgcggaggt cgcggagaca ctcttccact ccgcgttctc cgcgtctctg
     5941 cgtggtaatc tggatggccg ggtcaagccc ggccatgacg aagtagggta gggtaatgtc
     6001 cgacaagttg cgtatcctcc tcaccaacga cgacggcatc catgccaagg gtctggaggt
     6061 cgccgagacc attgcgcgcg cgctcaccga cgacgtctgg gtggtcgcgc ccgagaccga
     6121 acagtccggc tcctcccatt cgctgacgct ggtgcggccg ctgcgcctgc gcgcgatcga
     6181 cagccgccat ttcgccgtca ccggcacgcc caccgattgc gtgatgatgg cgacgaccca
     6241 tctgatgaag gacagccagc ccacgctggt gctgtccggc gtcaatcgcg gcgtcaacgc
     6301 ggccgacgac gtcacctatt cgggcaccat cgccggggcg atggaaggct gcgccctcga
     6361 cattccctcc ctcgccctgt cgcaagccta taatttcgag gagaagggcg aggtgcagtg
     6421 ggcctgcgcc gaaacgcacg ggccggatct gatccgcaag ctgatcgaga tcggctggcc
     6481 gcaggatgtg ctgatcaaca tcaatttccc cgactgtccg cccgacgcgg tgaagggtgt
     6541 cgagatcgtg gcgcagggta agcgcgacct gcaggaggcc gtgatcgaca ggcgcgtcga
     6601 tatgcgcggc tatccctatt tctggatcgg tttccgccgc gagcgggttt cggccaagcc
     6661 gggaacggat ttgggcgccc tctacgcgaa aaaaatctcc atcacgccgc tgcaactgaa
     6721 tctcaccgag acggcggtga tggaaaggct gaaacaagaa ctggatttca ccctcccgag
     6781 ggagggtcga acggcgaagc cgttcgagga ggggtaatgc gcgggcaaca gacccctcac
     6841 cgcccgcctt cgcactatgc gcttcggcgg actgcctctc cctcaagggg agaggcgagc
     6901 ggccgctgat cgccatgtcg ctccacgatc cccgccccat cgcgcttgtc atggcgctgc
     6961 gcaagcaggg gatcaccgac acgcgcgtgc tctccgcgat cgagcgctgc ccgcgcgagg
     7021 ccttcgtgga cgcgcctctg gcccattcgg cctatgacaa caccgcgctt cccatcgcct
     7081 gcggccagac catcagccag ccctatgttg tggcctacgc gaccgaggcc ctggaagtcg
     7141 gccccgccat gcgggtgctg gagatcggca ccggctccgg ctaccaggcc gccgtgctgt
     7201 cgccgctctg ccgcaaggtc tacaccatcg agaggcacaa gccgctgctg cgcgaggcgg
     7261 aagcccgctt caaggcgctc aagctcgaga acatcgtcac caagcacggc gacgggctga
     7321 acggctggcc ggaacaggcg ccgttcgacc gcatcctgct ttctgcggcg gtgccggagg
     7381 tgccgcaaat ccttatcgaa cagttaaagc ccggcggcat tctggtggcg ccggtggggc
     7441 cggcgacaat ttccagcatg gaaagttttt ctcaacattt aacgaagatg atccgatctg
     7501 agacaggttt gaagcgagag gcgctgatcc ctgtggtctt cgtaccgatg atgccgggag
     7561 tgccgcagga gccgcgaaca acccatggac gttcggatac cgcatgatgt ttggcgcggc
     7621 ctgatcctgg ccctgatcgt tcctgcgttc ctggcgggct gcacctccac tccgaaaacc
     7681 tcgtacgact ggggcgtcaa cacccgcctc caccaccgga cgcgtgtcgc ccagcagacg
     7741 catgccgccc cgcagaagac ctatagccgc agcgatacgt ccagctacag caataccgcc
     7801 aacccgcgcg tggttcccaa gccgcgcccc gagccgggct ggtatcagga tagcgcgcgc
     7861 cgcccgccgc gcgtcgccga aaacaatact cccgcgcccg tacaaacaaa catacaattt
     7921 gcttggcccg tgaagggaca ggtgctttcg aacttcggct cgacgaccgg cggcgaacgc
     7981 aacgacggca tcaacatcgc cgcatcctat ggcgagccga tccatgccgc agccgacgga
     8041 acggtcagct actccggcaa cgccctcaa
//
LOCUS       DASWPU010000176         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_1254, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000176 DASWPU010000000
VERSION     DASWPU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /gene="hisG"
                     /locus_tag="VGC27_04005"
     CDS             <1..300
                     /gene="hisG"
                     /locus_tag="VGC27_04005"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF013773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY0281772.1"
                     /translation="ATKYAQLTRGFFAHAGISDYRIVASAGATEGAPTAGTAEAIVDI
                     TTTGATLKANGLKILEDGVILKSQAQLAMSLAADWSGGMHAAAAALLKRLGAKLP"
     gene            complement(454..>1722)
                     /locus_tag="VGC27_04010"
     CDS             complement(454..>1722)
                     /locus_tag="VGC27_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY0281773.1"
                     /translation="GPLAAPTARIQVRELPSPGVVDFDAHKSVDAYLSKISGKEKARS
                     DAYFEGGYVLSVVDVLYTLVVSGLLLWLRISAWMRNFAAGITRHRFLQAPIYVVQYVA
                     VTTLVGLPLAIYEGFVREHAYGLSNQTFLQWSGDFLIQFVVSLIAFTVLLTLIYAAAR
                     AAKGRWWIWGTGITVAFMVVMLVISPIYIAPLFNHYAPLPESGLKESVLSMARANGIP
                     ADNIYVFDASRQSPRISANVSGFLGTTRISLNDNLLDRGTPSEVLAVLGHEMGHYVMN
                     HVGILLTWFGLLFLVGFVFIDRAYRGLTNLFGGSWDVRTIDDPAGLPLVMALFSVFML
                     AATPVTNTIIRTQEVQADIFGLNAVRQPDAFATVALKLSEYRKLDPSPWEEFVFYDHP
                     SGRTRIWEAMRWKAEHLNDPDILDGPISPQ"
BASE COUNT          372 a          524 c          540 g          286 t
ORIGIN      
        1 gccaccaagt acgcccagct cacgcgcggc ttcttcgccc atgccggcat ttcggattac
       61 cgcatcgtcg ccagcgccgg ggccaccgaa ggcgccccga ccgcaggcac cgccgaagcc
      121 atcgtcgaca tcaccaccac gggcgcgacg ctgaaagcca acgggctgaa aatcctggag
      181 gacggcgtca tcctgaaaag ccaggcgcag ctcgccatga gccttgccgc cgattggagc
      241 ggcggcatgc atgccgccgc cgccgcactg ctcaaacggc tgggggcgaa actgccttga
      301 tacggggctc taccctcccc ttgagggagg gtctaaattt gcatagcaaa tttcggggag
      361 gggtaatcgc tgtcgaaaac gcccctcccc gaaaaatctt cgctacgctt gatttttcta
      421 ccctccctca aggggagggt gaaagaccgc cattcactgc ggcgagatcg gtccgtccag
      481 gatgtcggga tcgttcaaat gttccgcctt ccagcgcatg gcttcccaga tgcgcgtgcg
      541 cccggaggga tgatcgtaga acacgaactc ttcccaaggc gagggatcga gcttgcggta
      601 ttcgctcagt ttcagggcga ccgtggcgaa ggcgtcgggc tggcgcaccg cattcagccc
      661 gaaaatgtcg gcctggacct cttgcgtacg gatgatggta ttggtgaccg gcgtggcggc
      721 cagcatgaac accgagaaca gcgccatgac caagggcagg ccggccggat cgtcgatcgt
      781 gcgcacgtcc caactgccgc cgaagagatt cgtcaaccct cggtatgccc ggtcgatgaa
      841 aacgaatccc accaagaaca gaaggccgaa ccaggtgagc aggatgccga catggttcat
      901 cacgtaatgg cccatttcgt ggcccagcac ggcgaggacc tcggaaggcg tgccgcggtc
      961 gagcagattg tcgttcagtg aaatgcgcgt cgtgcccagg aagccggaga cgttggccga
     1021 aatgcgcggg gactggcgcg aagcgtcgaa gacgtagatg ttgtcggccg gaatgccgtt
     1081 ggcgcgcgcc atcgacagga cggattcctt caagccgctt tcgggcagcg gcgcgtaatg
     1141 gttgaacagc ggcgcgatgt aaatgggcga tatcaccagc atgacaacca tgaacgcaac
     1201 cgtgatcccc gtgccccaaa tccaccagcg gcctttggcc gcgcgcgccg cggcgtaaat
     1261 cagcgtcaga agaactgtga aggcgatgag gctcacgaca aattgaatga ggaaatcgcc
     1321 gctccattgc aggaaggtct ggttcgacag cccgtaggcg tgttcgcgca cgaaaccttc
     1381 gtaaatcgcc agcggcagcc caaccagtgt cgtcaccgcc acatactgca cgacatagat
     1441 cggggcttgc aggaagcggt gtcgcgttat tccggcggcg aagttgcgca tccaggctga
     1501 aatccgcagc cacaacagca gcccggagac gacaagggtg tagaggacat cgacgacgga
     1561 aagaacgtat ccgccctcga aataggcgtc ggagcgcgcc ttctctttgc cgcttatctt
     1621 ggagaggtag gcgtccaccg acttgtgcgc atcgaaatcg acgacgccgg gggacggcag
     1681 ctcgcgaacc tggatacgcg cggtcggtgc ggccaagggg cc
//
LOCUS       DASWPU010000177         4677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_25717,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000177 DASWPU010000000
VERSION     DASWPU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25717"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VGC27_04015"
     CDS             <1..294
                     /locus_tag="VGC27_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281774.1"
                     /translation="AQAANGTCINTQRSYQAHPQGLHEVVIRSTMGKPRPALLLSTSC
                     IGLDKADSISVSSEFSCVGLGDTVVATKIDGHRQFCRVTRVAPYVPPQTPAKP"
     gene            complement(369..4019)
                     /gene="nifJ"
                     /locus_tag="VGC27_04020"
     CDS             complement(369..4019)
                     /gene="nifJ"
                     /locus_tag="VGC27_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEY0281775.1"
                     /translation="MQNDGAPAMLEVRESAVEANKPMIGRAEDAGVAPKKRAASPSQP
                     RMITVDGNEACASVAYRMNELAVIYPITPSSTMGELADQWMAEGRLNLWGGVLDVAEM
                     QSEAGAAGAVHGALQAGALATTFTASQGLLLMIPNMYKIAGELTPFVMHVSARTLASH
                     ALSIFGDHSDVMATRQTGFALLASASVQEAQDMAAVAQAASLKARVPFLHFFDGFRTS
                     HEVAKIVPLSDGDLRAMIDEETISQVRRRALSPDHPMLHGTAQNPDTYFQAVEARNPY
                     YDALPGLVQTEMDRFAAISGRQYKLFDYVGHPEAEQVVVIIGSGGETADLTSAWLNGH
                     GRKTGVLKVRLFRPFAIDAFVAALPPTTRAIAVLDRCKEPGAVGEPLFQDVVTALAEA
                     RALGQRPTAVEPVVIGGRYGLSSKEFTPAMVKAVYDELGREHPRRRFTVGITDDVTGL
                     SLAVDESFRVEAADERRAVFFGLGADGTVGANKNSVKIIADHTDLYAQGYFVYDSKKS
                     GAVTVSHLRFSPRPIQSAYLLQEAEFVACHHFVFLDRYDVLQYAAPGATLLLNAPMAA
                     DEVWSRLPRPVQQAILDKKLRLFVIDAGKVAHMAGMGGRINTVMQTCYFALADVLPKD
                     EAIASIKKAIEKTYGRKSRKVVEKNYAAVDMALAHLAEVVVPNRLDGPALPPVVPASA
                     PDFVRRVTAPMLAGRGDRLPVSAFPVDGAWPVGTSRFEKRNIADEIPAWEPSLCIQCN
                     KCTMVCPHASIRAKAVPAGLSEGTPPTLKSTPYKGKEVKGEHSYLLQVAPEDCTGCTL
                     CVAVCPVAGALAMTAKAPVLEAERANFDAFLALPDLDRADLRLNMKTTQLLKPLLEFS
                     GACTGCGETPYLKTLTQLFGDRMMVANATGCSSIFGGNLPTTPYTTDANGRGPAWSNS
                     LFEDNAEFGLGMRFALDRHREEARLLLTRLAGTLPEALVGALLGAPAKTDAAIRTQRG
                     QVTELKKLLVGRSDPASLRLAELADYLVDKLVWIVGGDGWAYDIGYGGLDHVLASGRN
                     VNILVLDTEVYSNTGGQQSKSTPLGATAKFAMTGKPRAKKDLGLIAMAYGDVYVAAVS
                     FGAKDAQTVRALQEAASYDGVSLVVGYAHCIHHGFDMASGLDRQKNAVAAGYWPLYRY
                     DPRKLAAGRPPLELDSAEPSLPLADFMAGETRFRMTADNDPIRYAELVKRAEVNIGRK
                     FETLKQLAPPDE"
     gene            complement(4041..4499)
                     /locus_tag="VGC27_04025"
     CDS             complement(4041..4499)
                     /locus_tag="VGC27_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HEY0281776.1"
                     /translation="MTATADSEAMAQPFVCELVGQIRAQDTYGAWEGKSDRELLEPFV
                     IDKEKARAIPIIGDPDPDTLSRLEQFYNAVALLIEKRCGIMASPVTQMSPEGFGRMVL
                     TCGRLVVINKVLRDVHRFGFASVDRIVKDGETLVGDALGWIERFPEVARA"
BASE COUNT          792 a         1546 c         1521 g          818 t
ORIGIN      
        1 gcccaggcgg ccaacggtac ctgcatcaac acgcagcgga gctaccaggc gcacccccaa
       61 gggctgcatg aggtcgtcat ccgcagcacc atgggcaaac cgcgcccggc cttgctgctt
      121 tcgacctcgt gcatcgggct cgacaaggcc gacagcattt ccgtgagttc ggagtttagt
      181 tgcgtaggcc tgggcgatac cgtggtcgcg accaaaatcg acggccaccg ccagttctgc
      241 cgcgtgaccc gcgtggcgcc ctatgtcccg ccgcaaaccc ctgcgaagcc ctgagcggcc
      301 ggctggacta cttaccgatg acacgccgga agacttgatg ctggcggtct cctcgcggcc
      361 ggcccttgtc attcgtccgg cggcgccagt tgcttcagcg tctcgaattt gcggccgatg
      421 ttgacctccg cccgtttgac gagttcggcg taccggatgg ggtcgttgtc ggccgtcatg
      481 cggaaacggg tttcgccggc catgaaatcc gcgagcggca ggctgggttc cgccgagtcg
      541 agttcgagcg gcggccgacc ggcggccagc ttgcgaggat cgtagcgata gagcggccag
      601 tagcccgccg cgacggcgtt cttctggcgg tcgagaccgg aggccatgtc gaagccgtga
      661 tggatgcagt gggcatagcc tacgaccagc gacacgccgt cgtaggaggc cgcctcttgc
      721 agagcgcgga cggtctgggc gtctttggcc ccgaacgaca ccgcggcgac atagacgtcg
      781 ccataggcca tggcgatgag gccgaggtcc tttttggcgc gcggcttacc ggtcatggcg
      841 aatttggcgg tggcgcccag tggcgtcgat ttcgactgct ggccgccggt gttggaatag
      901 acctcggtgt cgagcaccag gatgttcacg ttgcggccgg acgccagcac atggtcgagg
      961 ccgccatagc cgatgtcgta ggcccagccg tcgccgccga cgatccacac cagcttgtcg
     1021 acgagataat cggccagttc ggcgaggcgc aaagaggcgg gatcgctgcg gccgaccagc
     1081 aacttcttca gctccgtcac ttgcccgcgt tgcgtgcgga tggcggcatc ggttttcgcg
     1141 ggcgcgccca gcagggcgcc caccagcgct tccggcaagg ttccggctag gcgggtgagc
     1201 aggaggcggg cctcctcgcg gtgacggtcg agcgcgaagc gcatgcccag gccgaattcg
     1261 gcattgtctt cgaacagcga attggaccag gcgggaccac ggccgttggc gtcggtggtg
     1321 taaggcgtgg tcggcaggtt gccgccgaag atcgacgagc atccggtggc gttggccacc
     1381 atcatgcggt cgccgaacaa ctgggtcagg gtcttgagat aaggggtctc gccgcagccg
     1441 gtacaggccc ctgagaactc cagcagcggt ttgagcaact gcgttgtttt catgttgagg
     1501 cgcagatccg cgcgatcgag gtcgggcagc gccaggaagg cgtcgaaatt ggcccgttcc
     1561 gcttccagca ccggtgcctt ggcggtcatc gccagcgccc cggcgacggg gcacaccgcg
     1621 acgcacagcg tacagccggt gcaatcttcg ggcgcgactt gcagcagata gctgtgttcg
     1681 cctttcactt ccttgccttt atagggtgtc gacttcaacg tcggcggcgt tccctccgac
     1741 aggccggccg gcaccgcctt ggcgcggatg gaagcatgcg gacagaccat cgtgcatttg
     1801 ttgcactgaa tgcacagcga cggttcccaa gccgggatct cgtcggcgat gttgcgcttc
     1861 tcgaagcgcg aagtgccgac cggccaggcg ccatcgaccg gaaaagcgct gaccggcaac
     1921 ctgtcgccgc ggccggccag catcggcgcg gtcacacgcc gcacgaaatc gggcgcggaa
     1981 gcgggaacca ccggcggcaa ggcggggccg tcgaggcggt tcggcactac cacttcggcc
     2041 agatgcgcca gcgccatgtc gacggcggca taattcttct cgaccacctt gcggctcttg
     2101 cggccatagg tcttttcgat cgccttcttg atcgaggcga tcgcttcgtc cttgggcagc
     2161 acgtcggcca gggcgaagta gcaggtctgc atgacggtgt tgatgcgccc gcccatgcct
     2221 gccatgtgtg cgaccttgcc ggcgtcgatg acgaagagcc tgagtttttt gtcgaggatc
     2281 gcctgctgca ccggacgcgg cagcctgctc catacctcgt ccgccgccat cggcgcgttg
     2341 agcagcaagg tggcgcccgg cgcggcgtat tgcagaacgt cgtagcggtc gagaaagacg
     2401 aagtgatggc aggcgacgaa ctccgcctcc tgcaacagat aggccgattg gatcggccgc
     2461 gggctgaagc gcagatgcga aaccgtcacc gcccccgatt tcttcgaatc gtagacgaaa
     2521 tagccctggg catagagatc ggtgtggtcg gcgatgatct tgaccgagtt cttgttggcc
     2581 ccgacggtgc cgtccgcgcc caggccgaag aacaccgcgc ggcgctcgtc cgccgcctcc
     2641 acccggaagc tttcgtccac ggcgagcgac aggccggtaa catcgtcggt gatgccgacg
     2701 gtgaagcgcc gcctgggatg ttcccgtccc agctcgtcgt aaacggcctt gaccatcgcc
     2761 ggcgtgaatt cctttgaaga caagccgtag cggccgccga tcaccaccgg ctcgaccgcg
     2821 gtcggccgct gacccagggc acgggcctcg gccagcgccg tcaccacgtc ctgaaacagc
     2881 ggctcgccga cggcgccggg ttccttgcag cggtcgagca cggcgatggc ccgcgtcgtc
     2941 ggcggcagcg ccgccacgaa agcgtcgatg gcgaagggcc ggaacagcct caccttcaag
     3001 acgccggtct tgcgcccgtg gccgttcaac caggccgagg tcaagtcggc ggtctcgccg
     3061 ccggagccga tgatcaccac cacctgctcg gcctcgggat ggcccacata atcgaacaac
     3121 ttgtattgcc gcccgctgat ggcggcgaaa cggtccatct cggtttgcac caggccgggc
     3181 aaggcgtcgt aatagggatt gcgcgcttcg accgcctgga aataggtgtc ggggttctgc
     3241 gccgtgccgt gcagcatcgg atggtccgga ctgagcgccc gccggcgcac ctggctgatc
     3301 gtttcctcgt cgatcatcgc ccgcaaatcg ccgtccgaca gcggcacgat tttcgccacc
     3361 tcgtgcgaag tgcgaaagcc gtcgaaaaaa tgcaggaacg gcacgcgcgc tttgaggctg
     3421 gcggcctgcg ccaccgccgc catgtcctgc gcctcctgca ccgaggccga agccagcaag
     3481 gcaaagccgg tctggcgcgt cgccatcacg tccgaatgat cgccgaaaat cgacagggcg
     3541 tgacttgcca gcgtccgcgc gctcacatgc atgacgaacg gcgtcaactc gccggcgatc
     3601 ttgtacatgt tggggatcat cagcaggagc ccctgcgaag ccgtgaacgt cgtggccagc
     3661 gccccggcct gcaacgcccc atgcaccgct ccggctgcgc ccgcctccga ctgcatttcc
     3721 gcgacgtcca gaaccccgcc ccaaagattg aggcggccct ccgccatcca ctggtccgcc
     3781 agttcgccca tcgtcgacga aggcgtgatc ggatagatca ccgccagttc gttcatccga
     3841 taggcgaccg aggcgcacgc ctcgttgccg tccaccgtga tcatccgagg ctgcgatggg
     3901 ctggccgctc tcttcttcgg cgcgacaccg gcatcctcgg cccggccgat catgggcttg
     3961 tttgcctcca ccgcactttc tctcacttcc agcatggccg gcgctccgtc attttgcact
     4021 ccctcatcgc aaggcgctca tcaggccctg gccacttcgg gaaaacgttc gatccagccc
     4081 aaggcgtcgc cgaccagcgt ctcgccgtcc ttgacgatcc tgtccaccga agcaaagccg
     4141 aagcggtgaa cgtcgcgcag caccttgttg atcaccacca agcgtccgca ggtcaggacc
     4201 atgcgtccga atccttcggg cgacatctgg gtgaccggcg aggccatgat cccgcagcgc
     4261 ttttcgatca gtagggcaac ggcgttgtag aattgctcca gccgcgacag tgtgtcggga
     4321 tcgggatcgc cgatgatcgg aatcgcgcgc gctttttcct tgtcgatgac gaaaggctcc
     4381 agcagctccc ggtcgctctt gccttcccag gcgccataag tgtcctgggc gcggatttgc
     4441 ccgaccaatt cgcacacgaa cggctgggcc atggcttcgc tgtcggcggt ggcggtcatc
     4501 ggcgatcctc aatcctcaag aaagccgagg agggggcggt cgcggttcat cagcttgcgc
     4561 agccagggcg gcggcgaacc gcgcaacatg gtctgaacgc gccggatgac gtcggcgatg
     4621 gcctccggcg ccgggagctt gagcggatag acgcggttgt tgacgacgcg ggccgcc
//
LOCUS       DASWPU010000178         6314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_32198,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000178 DASWPU010000000
VERSION     DASWPU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32198"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2351
                     /gene="bamA"
                     /locus_tag="VGC27_04030"
     CDS             <1..2351
                     /gene="bamA"
                     /locus_tag="VGC27_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY0281777.1"
                     /translation="LVASFAVGATATAQELNPAEPAQPGASDQAAAPAAAEETRVVRR
                     MVVLGTQRIEPATVLSYITLHVGDTYSEAAIDRALKALFATGLFSDVKIRWDGSTVTV
                     NVVENPIINQVAFEGNSKVTTKDLTKEVQLKPRMVFTRAKVRSDIQRIIELYRRNGKF
                     AASVDPQIIQRPQNRVDLIFSINEGPTTGVARINFVGNKIFDDDTLREQIATVESAWW
                     RILATNDNFDPDRLLFDREQLRRFYVSHGYADFHVISAVAELTPDHSAFYITFTVDEG
                     PKYKFGKIEINSKIKELPTQTLLPLVKINAGAVYDENLVESAKDALIGAVGSKGYANA
                     DVGVRLRRNIETKTIDLIMNIEPGPRVYVDKISVVGNTRTLDRVIRRELRFAEGDAFN
                     RQLVDRSRTRIRALGFFRDVTIKNSPGSRRDKTDITVAVAEQSTGELSLGAGYSSTSS
                     FVGEFAYTERNLFGRGQYMRASIAMSTLSKQFQFSFTEPWFLDRPLAAGFDLYKWITN
                     YNQADYQGDATAASLRFRFPTSEYGSIGLHYTYRLNKVTPYSSAPLEVQLAAGSTRTS
                     ELGYSFIFNTLDDVIKPTSGIVFAFSQDFAGVGGNLKFLKTEGTLATYRKLIGDSFVG
                     SLTLSSGFITGYGGMTIPIQERYFKGGDSFRGFKLAGIGPRDITVSGDAGAVGGDFFA
                     IGTAEVRLPDILPADYGMKFSLFSDFGTLGHLDSAASRFCGTVGGYTGCIKDNLALRV
                     STGISIGWKSPFGPVQIDLGVPLVKAPYDKTEFIHFSAGTGY"
     gene            2356..2964
                     /locus_tag="VGC27_04035"
     CDS             2356..2964
                     /locus_tag="VGC27_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY0281778.1"
                     /translation="MTKKPFIACNILMAGLLLFGAAGLDAANAAPPPPGGAPTARILM
                     INMRQVMEASKVGQDIRRQVELLKQQASKELNGEGAALKNEETQLQQQAAILAPDVKA
                     RRIKDFQVRATAFQQKIQKRGNLIQGGVLKAQQQIEQALGPILEGIMKERGATLLLDR
                     SSILLAPNSIDVTQLVVQRLDMKMPTVKVEPTEPPEGMQQQQ"
     gene            2971..4047
                     /gene="lpxD"
                     /locus_tag="VGC27_04040"
     CDS             2971..4047
                     /gene="lpxD"
                     /locus_tag="VGC27_04040"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY0281779.1"
                     /translation="MRGDALDLAAMADPRFYDNRGPYTLAEVCAKAGVELPAGADGAA
                     KIDDLASLSGAAASHLAFFADEGAGTEFTQTAAGFCFVPVKIRKAVEPPAHLVTVACA
                     SVQHAFAAAANLFYPEWNLDDWPRQRTPVDPSAEIGEHVVLGPGVVVGAHARIGDGTR
                     IAPNTVIGRGVAIGRDCEIGSNVSISHAYLGDRLLILPGAQIGQPGFGFASNAEGHIK
                     IPQLGRVIVQDKVEIGACTTIDRGALGDTVIGEGTKIDNLVQIGHNTHIGRHCVFVGL
                     VGVAGSVTLGDFVVLGAQAGVADHARVGSGARIAGRGAAVPGQELEGGRDYGGLPAKP
                     VKEWMRELHAVSALVKKPKQGGHD"
     gene            4040..4537
                     /gene="fabZ"
                     /locus_tag="VGC27_04045"
     CDS             4040..4537
                     /gene="fabZ"
                     /locus_tag="VGC27_04045"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY0281780.1"
                     /translation="MTEKHSSHPILDIEDIKGLLPHRAPFLFVERLTDIVTHESATGH
                     KAVGYNEPYFQGHFPDHPVMPGVLIVEALAQTAGALVIHSLGLTSENRVVYFMTIEKA
                     RFRKPVRPGDMLQMRVKALRRRGPVWRFAGEAYVGGMLCAEAEFSAMIQDSANGESDP
                     SDGNR"
     gene            4503..5315
                     /gene="lpxA"
                     /locus_tag="VGC27_04050"
     CDS             4503..5315
                     /gene="lpxA"
                     /locus_tag="VGC27_04050"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY0281781.1"
                     /translation="MAKAIHPTAIVEDGATLGAGVEIGPFCIVGPQVKLGDGVRLISH
                     AVVAGDTELGARCVVHPGAVLGGESQIHGNDAAGTRLVVGEDTVIRECVSFSLGSAKG
                     HRLTEVGARGFFMAYSHIGHDCRVGNDVTFANGAQLAGHVDIGDGVILGGLSAVQQFG
                     RIGRGAMLGGVSGCNEDIIPYGIAFGTHSKLTGLNLVGLKRRGIPRASIHALRAAFRA
                     IFHETEGSLADRARKARETWAGIAEVQEIADFILAPAKRPICTARRRGAAEE"
     gene            5315..6160
                     /gene="lpxI"
                     /locus_tag="VGC27_04055"
     CDS             5315..6160
                     /gene="lpxI"
                     /locus_tag="VGC27_04055"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEY0281782.1"
                     /translation="MPPEAAKALGIVAGGGELPLAIAESARENGREVFVVGLLGSADE
                     WVSSYPHEWASLGEVGKVIKALHTHDCAEVLLAGKLERPNFSSLKLDAKGVLAVPRVI
                     AAARKGDDALLRAVVDLFEREGFHIVGTSEAAPGLLAPPGQIGRIAPSEESEADIVSA
                     FRIVRAMGALDIGQAAIVCGGLALAVEAAEGTDAMVARIASLPEHFRGTPARRRGVLA
                     KAPKPIQDRKTDLPVIGVQTVQGAAAVGLAGIAVEAGGALILGKRAVIEEADKLGLFL
                     VGVAR"
BASE COUNT         1163 a         1947 c         2064 g         1140 t
ORIGIN      
        1 ttctggtcgc ctcttttgcc gtcggggcga cggctaccgc gcaggagttg aatcccgccg
       61 aacccgctca acctggcgcc tcggatcagg ctgcggcgcc tgccgcggcg gaagagacgc
      121 gcgtcgtcag gcggatggtg gtcctgggca cgcagcgcat cgagcccgca acggttctct
      181 cctacatcac cctgcacgtc ggcgacacct acagcgaggc ggccatcgac cgcgccctca
      241 aggcgctgtt cgcgacgggg ctgttttcgg atgtgaagat ccgctgggac ggctcgacgg
      301 tgacggtgaa cgtggtcgag aatccgatca tcaaccaggt tgcgttcgag ggtaacagca
      361 aggtcacgac caaggacctt accaaggaag tccagctcaa gccgcgcatg gtcttcacgc
      421 gcgccaaagt gcgatccgac attcagcgca tcatcgagct gtatcgccgc aacggcaagt
      481 tcgcggccag cgtcgatccg cagatcatcc agcgcccgca aaaccgcgtc gatctgatct
      541 tttccatcaa cgaggggccg accacgggcg tcgcgcgcat caatttcgtc ggcaacaaga
      601 ttttcgacga cgacacgctg cgcgaacaaa tcgccaccgt ggaatcggcc tggtggcgca
      661 tcctggcgac caacgacaat ttcgatccgg accgtcttct gttcgaccgc gaacaactgc
      721 gccgcttcta cgtgagccac ggttatgcgg atttccacgt catttccgcc gtggccgaac
      781 tgacgccgga ccacagcgcg ttctatatca ccttcaccgt cgatgaaggt ccgaaataca
      841 aattcggcaa gatcgagatc aattcgaaga tcaaggaact tcccacccag acgctgcttc
      901 cgctggtaaa aatcaacgcc ggagccgtgt acgacgagaa cctggtggag agcgcgaagg
      961 acgcgctgat cggcgcggtc ggcagcaagg gttacgcgaa cgcggacgtc ggcgtgcggc
     1021 tcaggcggaa tatcgagacg aagaccatcg acctgatcat gaacatcgag ccggggccgc
     1081 gcgtctacgt cgataagatc agcgtcgtcg gcaacacccg tacgctcgac agggtcatcc
     1141 ggcgcgaatt gcgatttgcc gaaggcgacg ccttcaaccg ccagttggta gaccgttcgc
     1201 gcacccgtat ccgcgccttg ggcttcttca gagacgtgac gatcaagaat tcgccgggtt
     1261 cgcgccggga caagacggac atcacggttg cggtggcgga gcaatcgacg ggcgaactgt
     1321 cgctcggcgc gggctattcg tcgaccagtt ccttcgtcgg agagttcgcc tacacggaac
     1381 gcaatttgtt cggccgcggc cagtacatgc gcgccagcat cgcgatgtcc acgctgagca
     1441 agcagttcca attcagcttc accgagcctt ggttcctcga tcgtcccttg gcggccggtt
     1501 tcgacctcta caaatggatc acgaactaca atcaggccga ctatcaaggc gatgcgaccg
     1561 cggcatctct gcgcttccgc tttccgacgt ccgagtacgg cagcatcggc ctccactaca
     1621 cgtacagact caacaaggtg acgccataca gttccgcgcc gctggaagtg cagctcgcgg
     1681 cgggttcgac cagaacttcc gagttgggct attcgttcat tttcaacacg ctggatgacg
     1741 tcatcaaacc gacgagcggt atcgtcttcg cgttcagtca ggatttcgcc ggcgtcggcg
     1801 gcaacctgaa atttttgaag acggagggaa ctctcgcgac ctaccgcaag ctgatcggcg
     1861 attcctttgt cggttcgctg acgctgtcct caggcttcat caccggttat ggcggtatga
     1921 ccattccgat ccaggagcgt tatttcaagg ggggcgacag cttccgaggc ttcaagctgg
     1981 ccggtatcgg tccgcgcgac attaccgtgt ccggcgacgc cggcgccgtg ggcggagatt
     2041 tcttcgcgat cggcacggcg gaagtccgct tgccggacat cttacccgcc gactacggta
     2101 tgaaattttc gctgttttcg gatttcggta cgctcggtca tctcgattcc gccgcatcga
     2161 gattttgcgg tactgtgggc ggttataccg gctgcattaa ggacaatctg gcgttgcgtg
     2221 tatccaccgg catcagcatc ggctggaaat cgcccttcgg cccggtccaa attgaccttg
     2281 gcgtgccgct ggtcaaggca ccttatgaca agaccgaatt catccacttc agcgccggca
     2341 cggggtacta gatccatgac gaagaaaccg tttatcgcct gcaacatctt gatggctgga
     2401 ctgctgctct tcggagcggc gggccttgac gccgcaaatg cggcaccgcc gcctccgggc
     2461 ggagcgccca cggcgcgtat cctgatgatc aacatgcgcc aagtgatgga agcgtccaaa
     2521 gtcgggcagg acatccggcg ccaggtcgaa ctgctcaagc agcaggcctc caaggagctg
     2581 aacggcgaag gcgcggctct gaagaacgaa gaaacgcagc tccagcagca ggccgccatt
     2641 ctcgctcccg atgtcaaggc gcgcaggatc aaggattttc aggtgagggc cacggctttc
     2701 cagcagaaga tccagaagcg cggcaatctc atccagggcg gcgtcctgaa ggcgcaacag
     2761 cagatcgaac aggcccttgg cccgatcctg gaaggcatca tgaaagaacg cggcgccacg
     2821 ctccttttgg atcgttcttc cattcttctg gccccgaatt cgatcgacgt gacccagctg
     2881 gtcgtccagc gcctcgacat gaaaatgccg accgtgaaag tggagccgac cgagccgccc
     2941 gaaggtatgc agcagcagca ataagatcag gtgcgcggcg acgcgctgga cttagcggcc
     3001 atggccgacc ctcgtttcta tgacaatcgc ggtccttaca cgctggccga agtctgcgcc
     3061 aaggccggcg tcgagcttcc ggccggcgcc gacggcgcgg ccaagatcga cgatctggcc
     3121 agcctgtccg gcgcggctgc ctcccacttg gcgtttttcg cggacgaggg cgccggaacg
     3181 gaatttacgc agacggcggc cgggttctgc tttgtgccgg tcaagatccg caaggcggtc
     3241 gagccgccgg cgcatcttgt gacggtcgcc tgtgcgtcgg tgcagcacgc ctttgccgcg
     3301 gcggcgaacc tgttctatcc cgaatggaat ctcgacgact ggccgcggca gcgcacgccg
     3361 gtcgatccga gcgctgagat cggcgagcat gtggtgctgg gtccgggcgt cgtcgtcggt
     3421 gcgcatgccc ggatcggcga cggcacgcgc atcgctccca acaccgtcat cggccgcggt
     3481 gtggcgatcg ggcgggattg cgaaatcgga agcaacgtgt cgatctccca cgcctatctg
     3541 ggcgaccggt tgctgatcct gccgggggcg cagatcggcc agccgggttt cggtttcgcc
     3601 agcaatgcgg aaggccacat caagattccg cagctcggcc gcgtgatcgt tcaggacaag
     3661 gtggaaatcg gtgcctgtac cacgatcgat cgcggggcgc tcggcgacac cgttatcggc
     3721 gaaggaacga agattgataa tcttgtgcag attgggcaca acacccatat cgggcggcat
     3781 tgtgtgttcg tcggcctggt cggggtcgcg ggcagcgtga cgctcggcga ttttgtcgtg
     3841 ctgggagcgc aggccggggt cgccgatcat gctcgggtcg gcagcggggc gcggatcgcg
     3901 gggcgtggag cggctgtgcc cggtcaggaa ctcgaaggcg ggcgcgacta cggcggtctc
     3961 cccgcaaagc cggtgaaaga atggatgcgt gagttacacg cggtatccgc actggtgaaa
     4021 aagccgaagc agggcggtca tgactgagaa gcattcatcc catcccatcc tcgatatcga
     4081 agacatcaaa gggttgctgc cgcaccgcgc gccgttcctg ttcgtcgagc ggctgaccga
     4141 tatcgtgacc cacgaaagcg ccaccggtca caaggcggtc ggctacaacg aaccgtattt
     4201 ccagggccat tttccggatc atccggtgat gccgggggtg ttgattgtcg aggccctggc
     4261 gcagacggcc ggcgcgctgg tcattcattc cctgggtctg accagcgaga atcgcgtcgt
     4321 ctatttcatg accatcgaaa aagcgcggtt cagaaagccg gtccgcccgg gcgacatgct
     4381 gcagatgcgg gtcaaggcgc tgcgaaggcg cgggccggtg tggcgattcg ctggcgaagc
     4441 ctatgtcggc ggcatgttat gtgcggaggc ggaattcagc gccatgattc aggacagcgc
     4501 gaatggcgaa agcgatccat ccgacggcaa tcgttgaaga cggggcgacg ctgggggcgg
     4561 gcgtcgaaat cggcccgttt tgcatcgtcg ggccgcaggt gaaattgggt gacggcgttc
     4621 gcttgatctc ccatgccgtc gtcgccggtg acacggaact gggcgcgcgc tgcgtcgtgc
     4681 atccgggcgc cgtgctcggc ggagagtcgc agatccacgg caacgacgcg gccggcacgc
     4741 ggcttgtcgt cggcgaggat accgtcatcc gcgaatgcgt gtcgttcagc ctgggcagcg
     4801 ccaagggcca tcgcctcacc gaagtcggcg cgcgcggttt cttcatggcc tatagccata
     4861 tcggccacga ttgccgggtc ggaaacgatg tcaccttcgc caacggcgcg cagttggcgg
     4921 gtcatgtcga tatcggcgat ggcgtcattc tgggcgggct ctcggcggtg cagcagttcg
     4981 gccgcatcgg gcgcggcgcg atgctcggcg gcgtttccgg ctgcaacgag gacatcatcc
     5041 cctacggcat cgccttcggc acgcactcga aactgaccgg gctcaacctc gtcggcttga
     5101 agcgccgcgg cataccgcgc gccagcattc acgccttgcg cgcggcgttc cgcgccatct
     5161 tccacgagac ggaaggatcg ctggcggatc gcgcgcggaa ggcgcgcgag acctgggcgg
     5221 gtatcgccga agtgcaggag atcgccgatt tcattctcgc gccggcgaag cggccgatct
     5281 gcaccgcgcg ccggcgcggc gcggccgaag agtgatgccg ccggaagcag caaaagcgct
     5341 gggcatcgtc gccgggggcg gcgaacttcc tttggctatc gccgaaagcg cgcgtgagaa
     5401 cgggcgcgaa gtgttcgtcg tggggcttct cggttcggcc gacgaatggg tctcgtccta
     5461 tccgcacgaa tgggcctcgc tgggcgaggt gggcaaggtg atcaaggcgc tgcacaccca
     5521 tgattgcgcc gaggtgctgc tggccggcaa gctggagcgg ccgaatttct ccagcctcaa
     5581 gctcgacgcc aagggcgtac tcgccgtgcc gcgcgtgatc gcggcggcgc gcaagggcga
     5641 cgacgcgctg ctgcgcgccg tggtcgatct gttcgagcgc gaaggcttcc atatcgtcgg
     5701 gacgtccgag gccgcgcccg gactgctggc gccgccgggc caaatcggcc gcatcgcacc
     5761 ctccgaagag agcgaagcgg atatcgtttc cgcgttcagg atcgtccgcg ccatgggcgc
     5821 gctcgacatc ggccaggccg cgatcgtttg cggcgggctg gcgctggcgg tggaagcggc
     5881 ggagggaacc gacgccatgg tggcgcgcat cgccagcctg ccggagcatt ttcgcggcac
     5941 gccggccagg cgacgcggcg tgctggcgaa ggcgcccaag ccgatccagg accgcaagac
     6001 cgacttgccc gttatcggcg tgcagacggt gcaaggcgcg gccgccgttg gtctcgccgg
     6061 catcgccgtg gaagcgggcg gggcgctaat cctgggcaag cgcgcggtga tcgaggaagc
     6121 cgacaagctg ggcctgttcc tcgtcggcgt ggcacgctga ccgcaatgcc gagattggag
     6181 cgggttggga tcgaatggga ccaacacccc gtttgtcacg gcccgcgaaa gcgggccgcc
     6241 caggtgaagc cagctcctgt ctttcatata cggagctgcg ctattacttt ggcatcaagt
     6301 atttgtcgtc tgga
//
LOCUS       DASWPU010000179         4278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_74841,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000179 DASWPU010000000
VERSION     DASWPU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74841"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..2804)
                     /locus_tag="VGC27_04060"
     CDS             complement(66..2804)
                     /locus_tag="VGC27_04060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY0281783.1"
                     /translation="MNELQSRILDLPALVPAGHGAVACEGNSCRRLDLKDAQRIFRSG
                     EALVAHAAFVAGRLKTAPGKPLYDVLELFAFVRPGVPCVPSALGIARALGLGAPQTQE
                     DAARSLRASALRLLEDIAASHEAIRAELRPLVHTMAAAGWRWAAVIEGIIGSVVLQHA
                     PLAGFEVWRKLKEWEDEAPPGKPSSLPVALEEARVRLAQLVGGGGEARPEQRAYTDAA
                     SYAFSPRERAGAPRIALVEAGTGIGKTLGYLAPASLWAEKNGPGLWISTYTRNLQRQI
                     VQEIARLYPDPAERDEKAVVRKGRENYLCLLNFEEAVKRTTLAPGPRSVTLALVARWI
                     AATADGDMSGAGFPAFLAASFALREITDRRGACIYAACPHYRVCFIERIVRRARNAPI
                     VVANHALVIAQAAQDWLSVDETTDTPAERRVRYVFDEGHHLFDAADSGFAALVSGAEM
                     AELRRWVRGPEGRARSRRRGLEERLKDLVEDSESARDALQEAVAAAGALAGEGWMQRL
                     GSPHGPGEVFLAAAYGHVRARSDDKDALYSLEADTHPLGEQTIEAAKHLGLGLRRLAA
                     PLMRLAKSLRKQLNDRAAELEPYQRARIEAAARGLERRGRIILPAWIAMLEALETGEI
                     NDEFTDWFEIAREDGRDLDVGLERHWIDPTIPLANEVLAHAHGALITSATLRDAGDGD
                     GDWQSAEVRTGAHHLPEPPKRASFGSPFSYAEQSRVLIVRDVERRDPDALAAAYRELF
                     LASGGGALGLFTAVRMLRAVESRIAAPLADAGLTLYAQHVDRLDTGALVDLFRSEENS
                     CLLGTDALRDGVDVPGRSLRLVVFDKVPWPKPTILHKARRARFGKGYDDLLTRLRLKQ
                     AFGRLIRGASDKGCFVILESRTPTRLLAALSVEAPVLRCGLAEAVREIRGFLGEAR"
     gene            2913..3305
                     /locus_tag="VGC27_04065"
     CDS             2913..3305
                     /locus_tag="VGC27_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY0281784.1"
                     /translation="MALPAYGTFYVYILECADHSYYVGLTERTLEDRLAEHIDGTFQG
                     YTHERRPVKLVWSERFGRLTDAIARERQLKGWRRAKKEALIRGNFSVLPMLAKTAKTP
                     TEQSRAVDGSLSAPFDRLRVRGQSNPSC"
     gene            complement(3368..>4278)
                     /locus_tag="VGC27_04070"
     CDS             complement(3368..>4278)
                     /locus_tag="VGC27_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0281785.1"
                     /translation="GDNSYARREQGAGLGLAVVKRIIDAAGGEIGFDSEPGEGSTFWF
                     AVPAAGPAGQNAPAQSEQNAAPPSGLNLLVFVTDEAARKLLARALEPFGNKLVFAESA
                     ADAVARAGRDAFDAIIVTAKNADTLAAAPSVRAPVLTLLAPGDRAPVCAAELLRGPVD
                     ARQLYRALANICAAGQAAAAIPQETTAAIDAAAFAALEKSVGATTLIEILKSYIETTE
                     RLCTSLGAASAEANWQQAARLAQDIAGSSSGLGLLAMTAAARGFAAAARQGADAHTLR
                     NDAQMIVIEQERVRRALANLYPDLAA"
BASE COUNT          687 a         1456 c         1430 g          705 t
ORIGIN      
        1 ttcggtgggc actccccccg tgaacggggg gagaaagggt gagtataagt cctgccgccc
       61 gcccgtcacc gggcctcgcc aagaaaccca cggatttcgc gcaccgcctc ggccaggccg
      121 cagcgcaaca cgggcgcctc aaccgacagc gccgccagca gccgcgtcgg cgtccggctt
      181 tccaggatga cgaagcagcc cttgtcggac gcgccccgga tcagccggcc gaaagcctgt
      241 ttcaggcgca gccgggtcag aagatcgtcg tagcccttgc cgaagcgggc acgccgcgcc
      301 ttgtgcagga tggtgggctt gggccagggc accttgtcga acaccaccag cctgagcgag
      361 cggcccggca cgtccacccc gtcgcgcagc gcatcggtgc cgagcaggca ggaattctcc
      421 tcgctgcgga acagatcgac cagcgcgccg gtgtcgagcc ggtccacatg ctgggcataa
      481 agcgtcagcc cggcgtcggc cagcggcgcg gcgatcctgc tttcgaccgc gcgcagcatc
      541 cgcaccgccg tgaacaggcc gagcgcgcct ccacccgaag ccaggaacaa ttcgcgatag
      601 gcagcggcca gcgcgtcggg gtcgcgccgc tccacgtcgc gcacgatgag gacccgcgat
      661 tgctccgcat aggagaaggg cgagccgaag ctggcgcgct tgggcggttc gggcaggtgg
      721 tgggcgccgg tgcgcacttc ggcgctttgc cagtcgccgt cgccgtcgcc cgcgtcgcgc
      781 agcgtcgccg aggtgatcag cgcgccgtgg gcatgggcca gcacttcgtt cgccagcgga
      841 atggtgggat cgatccagtg ccgctccaat ccgacgtcga gatcgcgtcc gtcctcgcgc
      901 gcaatctcga accagtcggt gaactcgtcg ttgatttcgc cggtttccag cgcctccagc
      961 atcgcaatcc aggccggcag gatgatgcgt ccgcgccgct ccagcccgcg cgccgccgcc
     1021 tcgatgcggg cgcgctgata aggctcaagc tccgccgccc tgtcgttcaa ttgcttgcgc
     1081 aggcttttcg ccagccgcat caacggcgcg gccaggcgcc tcaagccgag gccgagatgc
     1141 ttcgccgcct cgatggtctg ttcgccgagc ggatgggtat cggcttcgag cgaatagagc
     1201 gcatccttgt cgtcgctgcg cgcccgcaca tggccgtaag ccgccgccag aaaaacttcg
     1261 cccggaccgt gcggactgcc cagccgttgc atccagcctt cgccggccag ggcgcccgcc
     1321 gccgcgaccg cctcttgcag tgcatcgcgc gcgctctcgc tgtcttccac cagatccttc
     1381 agccgttcct ccaggccgcg ccggcggctg cgcgcgcgcc cttccggccc gcgcacccag
     1441 cggcgcagtt cggccatttc cgcgccgctg accagcgccg cgaagccgga atccgccgcg
     1501 tcgaacagat gatggccttc gtcgaagacg tagcgcacgc gccgttcggc gggcgtgtcg
     1561 gtggtttcgt ccacgctcag ccagtcctgc gccgcctgag cgatgacgag ggcatgattg
     1621 gcgacgacga tgggcgcatt gcgggcgcgc cgtacgatgc gctcgatgaa acagacgcgg
     1681 taatgcggac aggccgcata gatgcatgcg ccgcgccggt cggtgatctc gcgcaacgcg
     1741 aagctcgccg ccaggaaggc gggaaagccc gcgcccgaca tgtcgccgtc ggcggtcgcc
     1801 gcgatccagc gcgccaccag cgccagcgtc acgctgcgcg gacccggcgc taatgtggtg
     1861 cgcttgaccg cttcttcgaa attcagcagg cagagataat tctcgcggcc tttgcgcacc
     1921 accgccttct catcgcgctc ggcgggatcg ggataaagcc gggcgatctc ctgcacgatc
     1981 tggcgctgga ggttgcgggt gtaggtcgaa atccacagcc ccggtccgtt cttctccgcc
     2041 cacaggctgg cgggggcgag atagcccagc gtcttgccga tgccggtgcc cgcttccacc
     2101 agcgcgatcc tgggcgcgcc ggcgcgctcg cgcgggctga aggcgtaact cgcggcatcg
     2161 gtataggcgc gctgttcggg gcgcgcttca ccgccgccgc cgacgagctg tgccagccgc
     2221 acccgcgctt cctccagcgc gacgggtagc gacgacggct tgccaggtgg cgcttcatcc
     2281 tcccattcct tcagcttgcg ccagacctcg aaacccgcga gcggtgcgtg ctgcaagacg
     2341 acagacccga tgatgccttc gatgaccgcc gcccagcgcc agcctgccgc cgccattgtg
     2401 tgaaccagag gacgcagctc ggccctgatc gcctcgtggc ttgcggcgat gtcttccagc
     2461 agcctcagcg ccgaggcgcg caaggagcgg gcggcgtctt cttgcgtctg cggtgcgccg
     2521 aggccgagcg cgcgcgcgat ccccagcgca ctcggcacgc aaggcacgcc aggccggacg
     2581 aaggcgaaca actccagcac gtcgtaaagc ggtttgcccg gtgcggtctt cagccgtccg
     2641 gcgacgaagg ccgcgtgcgc gaccagcgcc tcgccggaac gaaaaatcct ctgggcatcc
     2701 ttgaggtcca ggcgccggca ggagttgcct tcgcacgcca ccgccccatg ccccgccggg
     2761 acaagggcgg ggagatcgag tattcgcgac tgcaattcat tcattccctc accctgggct
     2821 tgtcgcaggg tgggcttgtc gaagggtgaa gagcaacttg aaagcacaat tgtgagatcg
     2881 ctatgcttag acatattcgg tatgggggca acatggcgct accagcctat gggacgttct
     2941 atgtatatat cttggagtgc gccgatcact cctattatgt cggtctcacg gagcgtactc
     3001 tcgaagaccg tttggccgag catattgacg ggacatttca gggttataca cacgaacgca
     3061 ggccagtgaa gctggtctgg tcggagcgat ttgggcgttt gacggatgcc attgcccgcg
     3121 agcgccagct aaaaggctgg cgtcgcgcga agaaagaagc gttgatccgc ggcaacttct
     3181 ccgtgctgcc tatgcttgcg aaaacggcaa agacgccgac agagcaatcg cgcgcggtgg
     3241 atggatcact ttccgcaccc ttcgacaggc tcagggtgag gggtcaatcc aatccttcat
     3301 gctgagcctg tcgaagcatg agcctgtcga agcatgagct tgtcgaagca tgagagagca
     3361 agaatcccta cgccgccaaa tcgggataga ggttcgccag cgcgcggcgg acgcgttcct
     3421 gttcgatgac gatcatttgt gcgtcgttgc gcagagtgtg cgcgtccgcg ccctgccgcg
     3481 cggcggcggc aaacccgcga gcggcggcgg tcatggcgag caggcccagg ccgctggagc
     3541 ttcccgcgat gtcctgggcg aggcgggcgg cttgctgcca attggcctcg gcgctggcgg
     3601 cgcccagcga ggtgcaaagc cgttcggtcg tctcgatgta ggatttcagg atttcgatca
     3661 gcgtcgtcgc gcccaccgac ttttccagcg ccgcgaaggc ggccgcatcg atcgccgccg
     3721 tcgtttcttg cgggattgcc gcggcggcct gtccggccgc gcagatattg gcgagcgccc
     3781 ggtagagctg acgtgcatcg accggcccgc gcagcaattc ggcggcgcag acgggcgcgc
     3841 ggtcgcccgg cgcgagcagc gtcaggacgg gcgccctcac gctcggtgcg gcggccagcg
     3901 tgtcggcgtt cttggcggtg acgatgatgg cgtcgaaggc gtcgcggccg gcccgggcta
     3961 cggcgtccgc cgcgctttcg gcgaagacca acttattccc gaacggttcg agggcccgcg
     4021 caagcaactt gcgtgcagct tcgtcggtta cgaagacgag caggttcagt ccggacggcg
     4081 gcgccgcatt ctgttcgctc tgtgccggag cgttttgtcc ggcgggcccg gcggccggca
     4141 cggcgaacca gaaggtggaa ccctcgcccg gctcgctgtc gaaaccgatt tcgccgcccg
     4201 ccgcgtcgat gatgcgcttg acgacggcaa gtccgagacc cgcgccctgt tcacgcctgg
     4261 cataggaatt gtcgcccg
//
LOCUS       DASWPU010000180         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_99470,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000180 DASWPU010000000
VERSION     DASWPU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99470"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1534)
                     /gene="glgA"
                     /locus_tag="VGC27_04075"
     CDS             complement(50..1534)
                     /gene="glgA"
                     /locus_tag="VGC27_04075"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEY0281786.1"
                     /translation="MTVRVLSVASEAYPLVKTGGLGDVAGALPAALAPYDIRLTTLIP
                     GYPAVMAALKESRLLHAYKDLLGVEAWLVGGTAGGLEVVVLDAPALFTRGGGLYSGPG
                     GKDWPDNWLRFGALGRAAADIVRGAVAGLHFDLLHAHDWQGAMAAAYLRFGKGTAAPS
                     IVTIHNLAFPGYFPAEVFPRLDLPPEAFAIEGVEYFGGVGFLKAGLWAADAITTVSPA
                     YAEQMRTPAFGMGFEGLLTTRRDRVSGILNGIDTLVWNPATDTNLAAVYDLRALHRRV
                     LNRRAIESEFGLNEGGGPLFCVVSRLTAQKGMDVLAGIADELVAMGGRMALLGSGDAK
                     LEAAFKAAAARHPGRFGVRIGYDERLSHLLQGGADAIVIPSLFEPCGLTQLYGLRYGC
                     VPVAARTGGLADTIIDANEAALAAGVATGFLFDEVTPDGLSHALRRALSLFGNREAWR
                     IMQKRGMKADFSWKRSGRHYAELYTRLLKAALRPQPPSPAPGPG"
     gene            complement(1531..2793)
                     /gene="glgC"
                     /locus_tag="VGC27_04080"
     CDS             complement(1531..2793)
                     /gene="glgC"
                     /locus_tag="VGC27_04080"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY0281787.1"
                     /translation="MDKSHSQPLARDTIAYVLAGGRGSRLNELTDKRAKPAVYFGGKA
                     RIIDFALSNAVNSGIRRIAVATQYKAHSLIRHLQQGWDFFRAERNESFDILPASQRVS
                     EAQWYEGTADAVFQNIDIIESYAPEFMVVLAGDHIYKMDYEVMLQQHVESGADVTIGC
                     LEVPRAEASCFGILAVDEKDVITEFLEKPADPPGIPGKPDVALASMGIYVFRTTRLFE
                     ELRRDAATPGSARDFGKDIIPYMVKNAKAVAHRFVDSCVRSGGEKNSYWRDVGTIDAY
                     WEANIDLTDVIPQLDLYDRSWPLWTYGEITPPAKFVHDVEGRRGSAVASLVSGDCIIS
                     GSHLRRCLIFTGVRSNSFSILEEAVVLPHCVIGRNARLRRVVLDRGVVIPEGLVVGED
                     PAVDAQRFRRTEKGICLITQTMINRLAL"
     gene            complement(3168..>3404)
                     /locus_tag="VGC27_04085"
     CDS             complement(3168..>3404)
                     /locus_tag="VGC27_04085"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF006691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY0281788.1"
                     /translation="AALIHLLAERFKCTQTVGRYKAEHKLPAADPRREARQIARLRKL
                     AHEAKLDPDFAEKFLNFIIKEVIRHHVAIRRERG"
BASE COUNT          634 a         1093 c         1092 g          585 t
ORIGIN      
        1 attcgcgaca aggacgtttg aatttcaaat gagcaggtcc ccgccagggt cagccggggc
       61 caggcgcagg ggacggcggt tgcggccgta aagcggcttt gagcaggcgc gtatagagtt
      121 cggcgtaatg ccgcccgctt cgcttccacg aaaaatcggc cttcatgccc cgcttctgca
      181 tgatgcgcca cgcttcccgg ttaccaaaaa gggagagcgc gcgccggagc gcatggctga
      241 ggccgtccgg cgtcacctcg tcgaacagaa aaccggtcgc cacgcctgcc gctagggcag
      301 cttcgttggc gtcgatgatc gtgtccgcca gcccgcccgt gcgcgccgcc accggcacgc
      361 agccgtagcg caacccgtaa agctgagtca ggccgcaagg ttcgaacagc gaagggatca
      421 cgatggcgtc ggctcccccc tgaagcaggt gcgaaagccg ttcgtcgtaa ccgatccgca
      481 cgccaaagcg cccgggatgg cgcgcggcgg cagccttgaa agcggcctcc agcttggcgt
      541 cgccgctccc caacagggcc atgcggccgc ccatggccac cagctcatcg gcgatgccgg
      601 ccagaacgtc catgcccttt tgcgcggtca gccggctcac cacgcagaag agcggcccgc
      661 cgccttcgtt caacccgaat tcggactcga tggcgcggcg gttgagcacg cgccggtgca
      721 gcgcgcgcag atcatagacg gcggcgaggt tcgtatccgt ggccggattc cacaccaacg
      781 tgtcgatgcc gttgagaatc ccggacacgc ggtcgcggcg cgtcgtgagc agcccctcga
      841 aacccatgcc gaaggccggt gtgcgcattt gttcggcgta ggccggactt accgtggtga
      901 tggcgtcggc cgcccacaga cccgccttca ggaagcccac gccgccgaaa tactcgacgc
      961 cctcgatcgc gaaagcctcg ggcggcagat ccaggcgcgg aaacacttcc gccgggaagt
     1021 agcccggaaa agccagattg tggatcgtga cgatgctggg ggccgccgtg cctttgccga
     1081 agcgcagata ggccgccgcc atcgcgcctt gccagtcgtg ggcgtggagc agatcgaaat
     1141 gcagaccggc gaccgcgccc cggacgatat cggccgccgc ccggcccagc gccccgaagc
     1201 gcagccaatt gtccggccag tccttgccgc cgggaccgga ataaagcccg ccgccgcgcg
     1261 tgaacaatgc cggcgcgtcg agcaccacca cttcaagtcc gcccgcggtg ccgcccacca
     1321 accatgcttc gacgcccaga agatccttgt atgcgtgaag gagccggctc tctttcagcg
     1381 ccgccatcac cgccgggtag ccggggatca gggtcgtcag ccggatatcg taaggcgcca
     1441 gcgcggccgg cagcgcgccg gcgacgtccc ccaagccgcc ggtcttgacc agcggataag
     1501 cttcggaggc gaccgaaagg acgcgcaccg tcataacgcc aagcggttga tcatcgtctg
     1561 ggttatcagg cagatgccct tttcggtgcg cctgaagcgt tgggcgtcga ccgccggatc
     1621 ctctcccacg accagtccct cggggatgac cacgccccgg tcgagcacca cgcggcgcag
     1681 ccgcgcgttc cggccgatga cgcagtgagg cagcacgacc gcctcttcca ggatcgagaa
     1741 actgttcgag cgcacgcccg tgaagataag gcagcggcgc aggtgggagc cggagatgat
     1801 gcagtcgccc gacaccagcg aggcgacggc gctgccgcga cggccctcga catcgtggac
     1861 gaatttggcc ggcggcgtga tttcgccata ggtccataac ggccagctcc ggtcgtaaag
     1921 atcgagctgc ggaataacgt cggtcaaatc gatgttggct tcccaataag cgtcgatggt
     1981 tcccacgtcg cgccaatagc tgttcttctc gccgccggag cggacgcagg aatcgacgaa
     2041 gcggtgagcg accgccttgg cgttcttcac catataggga atgatgtcct tgccgaagtc
     2101 gcgcgcggag cccggcgtcg cggcgtcgcg gcgaagttcc tcgaaaagcc gcgtcgtccg
     2161 gaagacatag atgcccatcg aggccagcgc gacgtcgggc tttcccggta tgccgggcgg
     2221 atcggcgggt ttttcgagaa attccgtgat cacgtccttt tcgtcgacgg ccaatattcc
     2281 aaaacagctc gcttcggccc gcggcacttc caggcatccg atcgtcacgt cggcgccgga
     2341 ctcgacgtgc tgctgcagca tcacctcata gtccatcttg taaatgtggt ccccggcgag
     2401 gaccaccatg aactccggcg cgtagctttc aatgatatcg atgttctgga aaaccgcgtc
     2461 ggcggtgcct tcgtaccatt gggcttcgga aacgcgctgc gacgccggca ggatgtcgaa
     2521 gctctcgttc cgttcggcac ggaagaagtc ccagccctgc tgcagatggc ggatcagcga
     2581 atgcgccttg tattgggtgg cgacggcgat gcgccggatg ccggagttca ccgcgttcga
     2641 cagggcaaaa tcgatgatcc tggccttgcc gccgaaatag accgccggct tggcccgctt
     2701 gtcggtgagt tcgttcagcc gcgatccgcg ccccccggcc agaacatagg ctatcgtgtc
     2761 tcgcgccagc ggctggctgt gtgatttatc cataggcggt tgtctccctg gggcacgcat
     2821 ctcggagagc gtgctcccgc tttatcgtcg acaggggtgc cagaatgagg atggccgcag
     2881 cctggcggac ttcttgtatc agcatgaccg acactaatgc acgctagtgt cgtggtttcg
     2941 aagttcgcag catgcgcact atcgggctgc aagcgaactt tgaaaccaaa acgacactag
     3001 gattcaatag gttgctagcg tccttcgatt ccgaaattcg cccgaggccg atatagagag
     3061 ggtgcgaatt tcggaatcgg gacactagcg cccccatcca gaaggaacaa ccgagagacg
     3121 atcatcggcg gcaattcaag ccggtgatga agatgggggc aaacggttca gccgcgttcg
     3181 cggcggattg ccacgtggtg gcggatcact tccttgatga tgaaattgag gaacttctcg
     3241 gcgaaatcgg gatcgagctt ggcctcgtgc gccagcttgc gcaggcgcgc gatctgcctg
     3301 gcttcgcgcc tgggatcggc ggccggcagc ttgtgctcgg ccttgtagcg gccgacggtc
     3361 tgggtgcatt tgaagcgctc ggcgagcagg tggatcagcg ccgc
//
LOCUS       DASWPU010000181         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_38505,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000181 DASWPU010000000
VERSION     DASWPU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38505"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VGC27_04090"
     CDS             complement(<1..330)
                     /locus_tag="VGC27_04090"
                     /inference="COORDINATES: protein motif:HMM:NF040446.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6499 domain-containing protein"
                     /protein_id="HEY0281789.1"
                     /translation="MPQADWRSTAAYDAKRKLDAPGFAFEFLRRNREFIRHHQRLERL
                     LKRNALSPRMRDAYARCWGLRFREGRTRLRETGDPLDGDGPAKCRPACLATARYCVPQ
                     YLYREIRP"
     gene            complement(444..722)
                     /locus_tag="VGC27_04095"
     CDS             complement(444..722)
                     /locus_tag="VGC27_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2285 domain-containing protein"
                     /protein_id="HEY0281790.1"
                     /translation="MSEPTSEPPVADSAPTDAVLTGYDEQHKITYLRLLDADADHADW
                     REVARIVLQIDPDKEPERAHRAWETHLARARWMTEQGYKFLLRGGPPN"
     gene            complement(762..1034)
                     /locus_tag="VGC27_04100"
     CDS             complement(762..1034)
                     /locus_tag="VGC27_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0281791.1"
                     /translation="MITGRQIRAARALLHWTQDMLAEKSLVALTALKRLESERGLGVH
                     EGTRDQVRRALEAAGTVFIDSPQGRGVIVLNKLPSIRVPRASHSRG"
     gene            complement(1272..1598)
                     /locus_tag="VGC27_04105"
     CDS             complement(1272..1598)
                     /locus_tag="VGC27_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HEY0281792.1"
                     /translation="MAIIGTFKKVTNGFEGEFAMLALQVKGVRIVAETNHSNEDAPTH
                     RIFVGRAEIGAAWTKSSEKNEEYHSLKLDDPSFTAPVYANLVKDKDGDTYSAIWSRRP
                     GKRNGN"
BASE COUNT          408 a          603 c          705 g          426 t
ORIGIN      
        1 cggtcgaatt tcacgataga ggtattgggg gacgcaatag cgggcggtag cgaggcaagc
       61 gggacgacac ttggcagggc catcgccgtc caaaggatcg ccggtttctc gcaatcgagt
      121 gcggccttcg cgaaatcgca aaccccaaca cctggcatag gcgtcgcgca ttcggggcga
      181 gagggcgttg cgtttcagga ggcgttcgag ccgttgatgg tggcggatga attcgcggtt
      241 ccgccgcagg aactcgaagg caaagcccgg tgcgtcaagc ttgcgtttgg cgtcatacgc
      301 ggcagtcgat cgccagtcgg cttgcggcat ggcgtaacac tccaattcgc cccaccgccc
      361 tgatgcaagc atggcgaaag tcctgtggat tgctagctga attcagcgca acgaccgttg
      421 caatgcgcgc aacgaccaaa gcgtcagttc ggcgggccgc cgcgcagcag aaacttgtac
      481 ccctgctcgg tcatccaacg ggcgcgtgca aggtgggttt cccatgcgcg atgcgcccgt
      541 tccggttcct tgtcaggatc gatctgcagc acgatccgtg caacctcacg ccaatccgca
      601 tggtcggcgt cggcatcgag caggcgcaga taggtgattt tgtgttgttc gtcatagccg
      661 gtgagaacgg catcggtagg cgcactgtcg gcaacgggcg gctcggaggt tggttctgac
      721 atttgtcgtc tccgaatggg gcgtccgcgc aagtgcagca ctcaaccgcg cgaatgtgaa
      781 gcacgcggaa cgcgtattga tggtagcttg ttgagtacta tgacgcctct accttgtggg
      841 gaatcgatga acacggtgcc ggcggcttcc agcgcgcggc gcacctggtc tcgcgttcct
      901 tcgtgcaccc ccagcccgcg ttcggattcg aggcgtttga gggcggtcaa cgcgacgagg
      961 gacttttccg cgagcatatc ctgcgtccag tgcaacagcg cccgtgcagc ccgaatctgt
     1021 cgacctgtga tcatgacgat cacgtccgac agtcggaatt tagacgccag aaatacgact
     1081 ataatagtcg cttttgggcc agacaacccc ggtatacgta gtaattcatc cgctttctcg
     1141 gtcgcttctc gaatcagtga ttcggacgtc gttcggtgat cttcgccgcg ctaatgggcg
     1201 ggaggcggag tgcgcactga caaagaaaaa ccccgcccgg cagaccggac ggggcttgga
     1261 ggcggggtgc ttcagttgcc gttacgcttg ccgggccggc gggaccagat cgcgctgtag
     1321 gtgtcgccgt ccttgtcctt gaccaggttg gcgtagaccg gggcggtgaa acttgggtcg
     1381 tcgagcttga gcgagtgata ctcctcgttc ttctccgacg atttggtcca ggcggcaccg
     1441 atctccgcgc ggccgacaaa gatgcgatgg gtgggggcgt cttcgttgct gtggttggtc
     1501 tcggcgacga tgcgcacgcc cttgacctgc agtgccagca ttgcgaactc tccttcgaac
     1561 ccgttggtga ctttcttgaa cgtgccgatg atagccatgt ctgttctcca cttgctgttt
     1621 cgagcccgcg accatcgcag cctcgatggc gatcggaagg ccgaaggcga tcgacgacgc
     1681 acccgctggc gggcttgagg gaggacggcg gactttcttg cctcgcgagg aatgggcttt
     1741 agcccagggg aagaaagtct gacggccccc ttgcgtctag gcgatcgagg cgcagccggt
     1801 ctttggcccg atcaaagcca agagaggcgg catggactcg cgtgccaagc agcatccggg
     1861 gagaactcat ggcgatgggt cggcggcaca agcaaggtcg ccgacgtctg ggtcgaagaa
     1921 agagaagcgc tggcgcagaa aaggaaaggc tgtatcggtg tgaccgaaca ccccaaagca
     1981 ttacctccat cgtgctgtcg gacgcgatgc ggtgctgctt ctggtcaagg tcggagaaga
     2041 tgggagtgtc cgctcaaacg cgagacgcaa tcagcgcgcc gttggtctgg caaggtcaag
     2101 ttggacggtg accgcgcgat ctagccgttg gccgggaagg aa
//
LOCUS       DASWPU010000182         2850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_105644,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000182 DASWPU010000000
VERSION     DASWPU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105644"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /gene="nifA"
                     /locus_tag="VGC27_04110"
     CDS             complement(<1..1598)
                     /gene="nifA"
                     /locus_tag="VGC27_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HEY0281793.1"
                     /translation="MSAGTPGPVESNLLAASSRSEMALGGVYEISKLLASPNRLEPTL
                     SGVLALLSSFLDMRHGLIALLDPEGKAEIVVGSGWSEANAKCYFEGLPERAIGQIVVT
                     KMPLVIPDVSRDPLFTNVDLPDFRKNDGAVISFIGVPIKQGDTVLGTLTIDRVRAKER
                     PVPYDTDVRFLTMIANLVGQTLRLHKVVAQDRERLLAEQARLAKGRQPEAKGKPKTTL
                     EGIIGNSPALQAVIEKVRIVAKSRSSIMLRGETGTGKEMFANAIHNLSPRKNGPFVKL
                     NCAALSESVLESELFGHEKGAFTGAISQRKGRFELAHGGTLFLDEIGEISASFQAKLL
                     RVLQEGEFERVGGTKSLKVDVRLVCATNRDLEADVAKGAFRADLYYRIAVVPIFLPSL
                     RERKGDIALLAGEFVKRFNREHGSELTLGPAALGILEACYFPGNVRELENCIRHTATM
                     ARSKNITDLDFACQNDGCLSAMLWKGADAGMPRPGYVPLPIAPGGGAGVAHRAPPPAS
                     ETPQAPAPVPRDCPAPGSCPVGTA"
     gene            complement(1910..>2850)
                     /locus_tag="VGC27_04115"
     CDS             complement(1910..>2850)
                     /locus_tag="VGC27_04115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEY0281794.1"
                     /translation="RHAQGVPPFAVLSCDNLPDNGQVLHRALVAFARQSDADLAAWIE
                     AEVVCPRTMVDSITPATDEELKQKVAEAIGLRDEAPVQREAFVQWVIEDILGPDAPDF
                     ASVGASLTSDVKAFEFAKLRLLNGAHSTLAYVGLLMGYSSVGEAMKDEHLAGFVGRMM
                     REDIVPSLHKTRGLDFESYIADILKRFRNPALTHKLYQIASDGSQKLPYRILGSLADA
                     LTAGRSIDRLAVPMAAWMQFVARDAKAGKTFADPLSDTLVKIGQACTGEARLDVPRFL
                     ALTDVFTRDLAGSQKFVATVGAAYDRITSGRFFAES"
BASE COUNT          522 a          914 c          894 g          520 t
ORIGIN      
        1 tcggcggtgc ccaccggaca ggagcctgga gccggacaat cgcgcggcac gggggcggga
       61 gcttgcggcg tttcacttgc cggcggaggc gcccgatgcg caacgccggc tccgccgccc
      121 ggcgcgatcg gaagcgggac atagcccggc cggggcattc ccgcgtcggc gcctttccac
      181 aacatcgcgg aaagacagcc gtcgttctga caggcgaaat cgagatcggt gatgttcttg
      241 ctgcgcgcca tcgttgcggt gtggcggatg cagttttcca gctcgcgtac gtttccgggg
      301 aagtagcacg cttccagaat tcccaatgcg gcgggaccga gcgtcaactc ggaaccgtgc
      361 tcgcggttga accgcttcac gaattcgccc gccagcagcg cgatatcgcc tttgcgttcg
      421 cgcaaagagg gcaggaaaat cggcaccacg gcgatgcggt aatacaaatc ggcgcggaag
      481 gcgcctttgg cgacatcggc ttccagatcg cggttggtcg cgcacaccag ccggacatcg
      541 actttcaggc ttttggttcc cccgacgcgc tcgaactctc cttcctgcaa aacgcgcagc
      601 agcttggcct ggaacgaagc cgaaatctct ccgatctcgt cgagaaaaag cgtgccgcca
      661 tgggcaagct cgaagcgccc tttgcgctgg cttatcgcac cggtaaaggc gcccttttcg
      721 tggccgaaca gttccgattc caggacgctc tcggacaacg ccgcgcaatt gagctttacg
      781 aacgggccgt tcttgcgcgg cgaaagattg tggatggcat tggcgaacat ctctttgccg
      841 gtgccggtct cgccgcgcaa catgatcgag gaccggctct tggcgacgat tctcactttt
      901 tcgatcacgg cctggagggc gggactattg ccgataatcc cctcgagcgt cgtcttgggt
      961 ttccccttgg cttccggctg acgccctttt gccagcctcg cctgctcggc caaaagccgt
     1021 tcgcgatcct gcgccacgac cttgtgcaag cgcagggtct ggcccaccag gttggcgatc
     1081 atggtgagaa agcgcacatc ggtgtcgtag ggaaccggcc gctcttttgc ccgtacccga
     1141 tcgatcgtca atgtccccag aacggtatcg ccttgcttga tcgggacgcc gatgaaggag
     1201 atcaccgcac cgtcgttttt tctaaaatcg ggaagatcga cgttggtgaa cagagggtcc
     1261 cggctgacat cgggaatcac cagcggcatc ttggtgacga cgatctggcc gatggcgcgc
     1321 tcgggcagcc cttcgaaata gcactttgcg ttggcttcgc tccaaccgga gccgacgacg
     1381 atctcggctt ttccctcggg atcgagcaga gcgatcaaac cgtgccgcat atccaggaaa
     1441 ctggacaaca gcgccagaac gccggacagc gtgggttcca gccggttcgg cgaggcaagc
     1501 aatttggaaa tttcgtagac gccgccgagc gccatctcgg accggctgct cgccgccaga
     1561 aggttggatt ccaccggacc tggcgtgcct gcgctcatga aagttccgtt tgcctattct
     1621 cgcgcctgaa ggcttcacct cgacccttcg ggaacaagac gcgctccgga ctcgttttca
     1681 tcatcgtgcg ctcgcactac accattgata tcgctgaaga ttcaccgcaa tctcgcagcg
     1741 ccggaacaga atgaccgccc tccaaaagtg tggctggaac gcagtggcca gcaaacctcc
     1801 cgcccactcc gtgcaaattt cacgccatgc ggaagagctg ccgggccgcc gccgaagagc
     1861 ggcgccgccg caccccgcac ggtctacccg gcaacgcgct tcgctaccgt cagctctcgg
     1921 caaaaaagcg gccggacgta atccggtcat aggccgcccc gacggtcgcg acgaacttct
     1981 ggctgccggc cagatcgcgc gtgaagacgt cggtcaaggc aagaaagcgc ggcacgtcga
     2041 ggcgcgcttc gccggtgcag gcctggccga tcttcaccag cgtatcggac agcgggtcgg
     2101 cgaaggtttt cccggccttg gcgtcgcgcg ccacgaactg catccaggcg gccatcggca
     2161 cggcgagacg gtcgatcgac cgccccgcgg tgagcgcatc ggcgagcgag cccaggatgc
     2221 ggtaaggcag cttctgggag ccgtcggagg cgatctgata cagcttgtgc gtcaaggcgg
     2281 ggttgcggaa gcgcttcagg atgtcggcga tataggattc gaagtcgaga ccgcgggttt
     2341 tgtgcagcga gggaacgatg tcctcgcgca tcatccgccc gacgaagccg gcgaggtgtt
     2401 cgtccttcat cgcttcgccg accgaggaat agcccatcag caggccgaca taggcgagcg
     2461 tcgagtgggc gccgttcagc agccgcaatt tggcgaattc aaaggccttc acgtcgctgg
     2521 tcaggctggc gcccacgctg gcgaaatcgg gcgcatcggg gccgaggatg tcctcgatga
     2581 cccattgcac gaaggcttcg cgctgcaccg gcgcctcgtc ccgcagcccg atcgcctcgg
     2641 ccactttctg cttcagctcc tcgtcggtgg cgggcgtgat gctgtcgacc atcgtgcgcg
     2701 gacaaacgac ttcggcttcg atccaggcgg cgagatccgc gtcggattgg cgggcgaacg
     2761 ccaccagcgc gcgatgcagg acctggccgt tgtccggcag gttgtcgcag ctcagcaccg
     2821 cgaatggcgg cactccctgc gcatgacgcc
//
LOCUS       DASWPU010000183         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_1365, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000183 DASWPU010000000
VERSION     DASWPU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..899
                     /locus_tag="VGC27_04120"
     CDS             165..899
                     /locus_tag="VGC27_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0281795.1"
                     /translation="MKYVWPIFKREFAAYFATPLAYVFIVIFLSAMGVFTFYVGHFYD
                     NGIANLTVFFGFHPWLYLFLVPAIAMRLWAEERKSGTLELLLTLPVPLWASVLGKYLA
                     AWAFTGIALLLTFPIWLTVNFLGSPDNGVILASYAGSFLMAGAYLAIGACISAATSNQ
                     VIAFVVSVAVCFLFTVAGAPLVLDFFSAWAPMPLTEAVASFSFLTHFDSITKGVIDLR
                     DVVFFGSLIALFLAANVVIVDLAKSR"
     gene            904..>2163
                     /locus_tag="VGC27_04125"
     CDS             904..>2163
                     /locus_tag="VGC27_04125"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEY0281796.1"
                     /translation="MKPLPRRLYAVAAIALAAVLFVGLNIAIDATIATARLDLTENGR
                     FTLSQGTRNIIAGLQEPVTLKFFYSKKAAAKYAQTRAYADRVRDLLRECTALSHGKVI
                     LEEVNPEPFTPDEDEATAYGLTAVPTDSGETVYFGLAGTNRIDGKEVISYFTPEREPF
                     LEYDLASLIYRLATPKKPKLAILTSLPLDTGIGGMAAVMRGTARPFALYQELAQTYDT
                     EMLDRDFTGIPSGADVLMIVQPGQLTDQQNYAIDQFVQKGGRALIFVDPNSELAASAS
                     GIDPSMTPPASSSLPRLFQAWGIGFYANKVIGDLQLAQRVQVSQSGEPVRYPVWLRLV
                     QKQFDTRDPITANLQVVNLANAGALFPRKDATTHFATLVASSPQAALLGSEEVRLNAR
                     PEDLMSSITPGGKPFAIAARISGPAKTA"
BASE COUNT          372 a          749 c          608 g          434 t
ORIGIN      
        1 atgaatattt ttcttcgtca cctctccctt tgtggggagg tcgaaaagtc tgagcgcagc
       61 gaagattttt cgggtggggg gccgcactct tcgccccgca ccgcccccca cccgaaattt
      121 gcttcgcaaa tttcgacctc cccacgaagg gggaggtaaa cccgatgaag tatgtgtggc
      181 cgatcttcaa gcgcgaattc gccgcctatt tcgcgacgcc gctggcttac gtcttcatcg
      241 tgatcttcct atcggcgatg ggcgtcttca ccttctatgt gggccatttc tacgacaacg
      301 ggatcgccaa tctgacggta ttcttcggct ttcatccctg gctctatctg ttcctggtgc
      361 cggccatcgc catgcggctg tgggcggagg agcgcaaaag cggcacgctg gaactgctgc
      421 tcactctgcc cgtgcctttg tgggcgagcg ttctcggcaa atatctggcg gcttgggcct
      481 tcaccggaat cgcgctgctg ctcaccttcc cgatctggct gacggtgaat ttcctcggct
      541 ctcccgacaa cggcgtgatc ctggcgagct acgccggcag tttcctgatg gcgggcgcct
      601 acctcgccat cggcgcctgc atctcggcgg cgaccagcaa ccaggtgatc gccttcgtgg
      661 tcagcgtggc cgtctgcttc ctgttcacgg tggccggcgc gccgctggtg ctcgacttct
      721 tcagcgcctg ggcaccgatg ccgctgaccg aagccgtcgc ttcgttcagc ttcctgaccc
      781 atttcgactc catcaccaag ggcgtgatcg atctgcgcga cgtggtgttt ttcggctcgc
      841 tgatcgcgct gttcctggcc gcgaacgtcg tgatcgtcga tctggcgaag agccggtgac
      901 gccatgaaac cgcttccccg ccgcctttac gccgtcgccg ccatcgcgct cgccgccgta
      961 ctgttcgtcg ggctcaacat cgccatcgac gccacaatcg ccaccgcccg cctcgacctg
     1021 accgaaaacg gccgtttcac tctctcgcaa ggcacgcgca acatcatcgc cggcctgcag
     1081 gaaccggtca ccctgaaatt cttctattcg aagaaggccg cggccaaata cgcccagacc
     1141 cgcgcttacg ccgaccgtgt gcgcgacctg ctcagggaat gcaccgcgct cagccacggc
     1201 aaggtcatcc tggaagaagt caatccggaa cctttcacgc cggacgaaga cgaagcgacc
     1261 gcctacggcc ttaccgccgt acccaccgat tccggcgaaa ccgtctattt cggcctggcc
     1321 ggcaccaacc gcatcgacgg caaggaggtc atttcctatt tcacgccgga acgcgaaccg
     1381 ttcctcgaat acgacctcgc ctcgctgatc taccgactgg cgacgcccaa gaagcccaag
     1441 ctcgccatcc tcaccagcct gccgctggac accggcattg gcggcatggc ggcggtgatg
     1501 cggggaacgg cgcgtccctt cgccctctat caggaattgg cccagaccta cgacaccgaa
     1561 atgctcgacc gcgacttcac gggcattcct tccggcgcgg atgtgctgat gatcgtgcag
     1621 ccgggccagc tgaccgacca gcagaattac gccatcgacc agttcgtgca gaaaggcggg
     1681 cgcgcgctga tcttcgtcga tcccaattcc gaactggccg cgagcgccag cggcatcgat
     1741 ccgtccatga cgccgccggc ttcgtcgtca ctaccgcgtc tgttccaggc ctggggcatc
     1801 ggcttctacg ccaacaaggt gatcggcgat ctgcagctag cgcagcgcgt acaagtctcc
     1861 cagagcggcg agccggtgag atatcccgtt tggctgcgtc tggtccagaa acagttcgac
     1921 acgcgcgatc cgatcaccgc gaatctgcag gtcgtcaatc tggctaatgc cggggcgctg
     1981 tttccgcgca aggacgcgac cacgcatttc gccaccttgg ttgcctcttc cccgcaggcc
     2041 gcgctgctcg gctccgaaga agtgcggctg aatgcgcggc cggaagatct gatgtcctcc
     2101 atcacccccg gcggcaagcc tttcgccatc gccgcgcgca tttccggccc ggccaaaacg
     2161 gcc
//
LOCUS       DASWPU010000184         4552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_1367, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000184 DASWPU010000000
VERSION     DASWPU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /gene="sufC"
                     /locus_tag="VGC27_04130"
     CDS             <1..693
                     /gene="sufC"
                     /locus_tag="VGC27_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY0281797.1"
                     /translation="LTLSVGTGKVHAIMGPNGSGKSTLSYVLAGREGYEITKGSIRYR
                     GADLTAMKPEERAAKGVFLAMQYPVEIPGVTTLTFLKTALNAQRRARHDSELDAMSVL
                     KLVRQRASALQVPEEMLKRALNVGFSGGEKKRLEIVQMAIFEPALAILDETDSGLDID
                     ALKLVADGVNALRAPDRSMLVITHYQRLLDFIVPDRVHVLAGGRIVAEGGKELALELE
                     AKGYAHFVKEPA"
     gene            690..1832
                     /gene="sufD"
                     /locus_tag="VGC27_04135"
     CDS             690..1832
                     /gene="sufD"
                     /locus_tag="VGC27_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY0281798.1"
                     /translation="MNAALPSIETRRSEALDNFRVRGMPHRRFEHWRYSDLRAILDAG
                     QVAQSGTAKWRIENLPEGVELFDLSAPADAPEWVRRHLGAVGNRPTMEEASLAFADGG
                     VAFRVMHGRKIAEPLRLQVSSGGNVRLLIVVEDGASLTLMEGHAADAGLLRNVGVEIA
                     LGPGAKLSHVRLAVGAPKSITVEHIAVAEARGACYRAHFTNFGSRLSRTELHIVLQEE
                     DAEAHLSGVSVLSGEGHADVTSHIEHAAGKTRSTQLFKKVAGGKSRAVYQGKITVRAG
                     ANGSDSRQTAKALLIGPRAEADLKPELIIFADDVKCGHGAAVGDLDTESLFYLRSRGI
                     PEKEARRLLIRAFLEEAVAEIENETLRAAIWHAVEEVLPRTMVVMP"
     gene            1829..3073
                     /locus_tag="VGC27_04140"
     CDS             1829..3073
                     /locus_tag="VGC27_04140"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY0281799.1"
                     /translation="MTAQVKRIPKAVFEPPRADFEILSRTVYGKPLVYLDSAASAQKP
                     RQVLDAMTAFAVSDYANVHRGVHYLAAAATQAYEAARETARHFLNAAHADEIVFTKSG
                     TEAVNLVSYSWLAPRIRPGDEIVLSVMEHHSNIVPWHFLRERQGAVLKWIDVRDDGSL
                     DMEAFDAAVSPKTKLVAVTHMSNVLGTVTPLKEIAARAHADGVPVLADGCQGAVHESV
                     DVCDLGVDFYALTGHKLYGPTGIGVLYAKREHLAAMRPFNGGGEMIREVARDAISYAD
                     PPARFEAGTPPIIESVGLAAALDYVASFGRDAVKAHEASLLSYAREAVREFNWLRVIG
                     DAPGKGAILSFEAEGMHAHDLATILDREGVAVRAGHHCAQPLMERFGVASTTRAAFAL
                     YNTREDVDALVKGLHKARKILG"
     gene            3073..3534
                     /locus_tag="VGC27_04145"
     CDS             3073..3534
                     /locus_tag="VGC27_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY0281800.1"
                     /translation="MAMDDGLRELYQDVILDHARHPRHFGALPGATHRGEGYNPLCGD
                     RVKVYLTVDGSGHIADIRFEGKGCAISQASASLMTDMVTGRSVAEAEKLMSGLLHLVR
                     GEDASDLVGDDRDRLNVLAGLSEFPMRVKCATLAWHTMKSALEGGDAAKTE"
     gene            3531..3896
                     /locus_tag="VGC27_04150"
     CDS             3531..3896
                     /locus_tag="VGC27_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEY0281801.1"
                     /translation="MNAELRNMATIGSSAITPEELEAFTAKLVAAMKTVYDPEIPVDI
                     YELGLIYKVDVADNKDVTVDMTLTAPGCPVAGEMPDMLKAALETVEGIGTVTVNMTFD
                     PPWTPYRMSDEAKLELNMY"
     gene            3896..4282
                     /locus_tag="VGC27_04155"
     CDS             3896..4282
                     /locus_tag="VGC27_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY0281802.1"
                     /translation="MSMSATTKPIRERPKAVRLTEAAAARIREIMAGAKGRYQGVRVG
                     VTNGGCAGMAYTMDYAESAQPLEEVVEDKGVKIFIDPKAILFLIGTEMDFVREKLGAR
                     FVFNNPNQTAACGCGESVSIAPAKAD"
     gene            complement(4279..>4552)
                     /locus_tag="VGC27_04160"
     CDS             complement(4279..>4552)
                     /locus_tag="VGC27_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281803.1"
                     /translation="VAGLVLGVVLLDFGVQSSMISNQHIIYALQPAARSRLNTVFTTG
                     MFIGGGIGSAGAMFIYHLSNWNWLCVYGAVMAGAALLLQIGGRGRN"
BASE COUNT          858 a         1385 c         1528 g          781 t
ORIGIN      
        1 ctgacgctca gtgtcgggac gggcaaggtg catgccatta tggggccgaa cggttccggc
       61 aaatctaccc tttcctacgt gctggcgggc cgcgaaggct acgagatcac gaagggcagc
      121 atcaggtatc gcggcgcgga cttgaccgcg atgaaaccgg aggagcgggc ggcgaaaggc
      181 gtgttcctgg cgatgcagta tcccgtggag attccgggcg tcaccacgct gaccttcctt
      241 aagaccgcgt tgaacgccca gcgccgcgcg cgccatgaca gcgaactcga tgcgatgagc
      301 gtgttgaagc tggtgcgcca gcgtgcaagc gcgctgcaag tcccggaaga gatgctcaag
      361 cgcgcgctca atgtgggctt ctccggcggc gagaagaagc ggctcgaaat cgtccagatg
      421 gcgatttttg aacccgcact ggccattctc gacgaaaccg actccggtct cgacatcgac
      481 gcgctgaagc tcgtcgccga cggcgtcaac gccctgcgcg cgcccgaccg ttccatgctg
      541 gtgatcaccc attaccagcg cctgctcgac tttatcgtgc cggatcgcgt gcatgtgctg
      601 gcgggcggac gcatcgtcgc cgagggcggc aaggaactgg ccctcgaact ggaagccaag
      661 ggctatgccc atttcgtgaa ggagcccgca tgaacgcggc cttgccgtcg atcgaaacgc
      721 ggcgctccga agcgctcgac aatttccgcg tgcgcggcat gccgcatcgc cggttcgagc
      781 attggagata cagcgatctt cgcgcgatcc tcgacgccgg ccaggtggcg cagtcgggta
      841 cggcaaaatg gcggatcgaa aacttgcccg aaggcgtcga acttttcgat ctctccgcgc
      901 cggcagatgc gccggaatgg gtgcggcggc atctcggcgc cgtcggcaac cggccgacga
      961 tggaggaggc ttccctggcc ttcgcggacg gcggggtcgc gtttcgggtc atgcacggac
     1021 ggaaaatcgc cgagccgctt cggttgcagg tttcgtccgg cggcaacgtg cgcctgctca
     1081 tcgttgtgga agacggcgct tcgctgacgc tgatggaggg ccacgccgcc gatgccggtc
     1141 tcttgcgcaa tgtcggcgtg gagattgcgc tcggccccgg ggcaaagctt tcccatgtgc
     1201 ggctcgcggt cggcgcgccg aaatccatca cggtggagca catcgcggtg gccgaagcgc
     1261 gcggtgcctg ctatcgcgcg catttcacga atttcggttc cagactgtcg cgcaccgagc
     1321 ttcatatcgt gctgcaggaa gaagacgccg aggcgcacct ttccggcgtc agcgtcctga
     1381 gcggcgaagg tcatgccgac gtgacgtcgc atatcgaaca tgcggcgggc aagacgcgga
     1441 gcactcagtt gttcaagaag gtcgccggcg gcaaatcgcg cgcggtctat cagggtaaga
     1501 tcacggtgcg cgcgggcgcc aacggttccg acagccgcca aacggcgaaa gctttgctga
     1561 taggcccgcg ggcggaagcg gatttgaaac cggagctgat catcttcgcc gacgacgtga
     1621 aatgcgggca cggcgccgcg gtcggcgatc tcgatacgga gtcgctgttt tatctgcgtt
     1681 cgcgcggcat tccggagaag gaagcgcggc gcctgctgat ccgcgccttc ctcgaagaag
     1741 ccgtggccga gatcgaaaac gagaccttgc gcgccgcgat ttggcatgcg gtcgaagagg
     1801 tattgccgcg tacgatggtg gtcatgccat gacggcgcag gtgaagcgaa tacccaaggc
     1861 cgttttcgaa ccgccacgcg cggatttcga aatcctgtct cgtacggtct acggcaagcc
     1921 gctcgtctac ctcgacagcg ccgcctcggc gcagaagccg cgccaggttc tggatgccat
     1981 gaccgcgttt gcggtgagcg attatgccaa cgtccatcgc ggcgtgcatt atctggccgc
     2041 ggcggcgacg caagcttacg aggccgcgcg cgagacggcg cggcatttcc tgaatgcggc
     2101 gcatgccgac gaaatcgtct tcaccaagag cggcaccgag gcggtcaatc tggtgtccta
     2161 ttcctggctc gcgccgcgca tccggcccgg cgacgagatc gtgctgtcgg tgatggagca
     2221 ccactccaac atcgtgccct ggcactttct gcgcgaacgg cagggcgcgg tcctgaaatg
     2281 gatcgatgtc cgtgacgacg gttcgctcga catggaagca ttcgacgctg ccgttagccc
     2341 gaaaaccaag cttgtggccg tgacgcatat gtcgaacgtg ctcggcaccg tcacgccgct
     2401 gaaagagatc gcggcgcgcg cgcatgccga cggcgttccg gtcctggccg acggttgcca
     2461 gggcgcggtg catgaaagcg tggacgtgtg cgatctcggc gtcgatttct atgcgctgac
     2521 cgggcacaag ctctatggcc cgaccggcat cggcgtgctt tacgccaagc gcgagcatct
     2581 ggcggcgatg cgcccgttca acggcggcgg cgagatgatc cgtgaagtcg ctcgcgatgc
     2641 gattagctat gccgatccgc cggcgcgctt cgaggccggc acgccgccga ttatcgaaag
     2701 cgtggggctg gcggcggcgc tggattacgt cgcctcgttc ggccgcgatg cggtcaaggc
     2761 gcatgaggcg tcgctgcttt cctatgcccg cgaggcggta cgggaattca attggctgcg
     2821 ggtgatcggc gatgcgccgg gcaagggcgc catactttct tttgaggcgg aggggatgca
     2881 cgcgcacgac ctcgccacta tattggatcg tgagggtgtc gcggtgcgcg cgggacatca
     2941 ttgcgcacag ccgctcatgg agcgcttcgg cgtggcctcc acgacacgcg ccgccttcgc
     3001 gctctacaac acgcgcgagg atgtcgacgc cttggtcaag ggcctgcaca aggcgcgaaa
     3061 gattttgggt tgatggcgat ggacgacggc ttgcgcgaac tctaccagga cgtgatcctc
     3121 gatcacgcgc gccatccgcg ccatttcggg gcgttgccgg gcgcgaccca taggggcgaa
     3181 ggttacaacc cgctctgcgg cgaccgcgtg aaagtttacc tcacggtaga tggcagcggc
     3241 cacatcgccg acatcaggtt cgagggcaag ggctgcgcca tcagccaggc ctctgcttcg
     3301 ctgatgacgg acatggtgac cgggcgcagc gttgccgaag cggaaaagct gatgagcggc
     3361 ttgctgcatc tggtaagggg cgaggacgcc agcgatctcg tgggcgacga ccgcgaccgc
     3421 ctgaatgttc tggccggtct gtcggagttc ccgatgcgcg tcaaatgcgc gacgcttgcc
     3481 tggcatacga tgaagtcggc cttggagggc ggcgacgccg cgaaaacgga atgaacgccg
     3541 aattgcggaa catggcgacg atcggctcct cggcaatcac gccggaggaa ctggaagcgt
     3601 tcacggccaa gctcgtggcg gcgatgaaga ccgtttacga tccggaaatc ccggtggaca
     3661 tttacgaact cgggcttatc tacaaggtgg acgtggccga caacaaggat gtgacggtcg
     3721 atatgacgct gaccgcgccg ggatgcccgg tggcaggcga aatgccggac atgctgaagg
     3781 cggcgcttga aaccgtcgaa ggcatcggca cggtgaccgt caacatgacc ttcgatccgc
     3841 cctggacgcc gtaccgcatg agcgacgaag ccaaacttga actgaacatg tactgatgag
     3901 catgagcgcg acgaccaaac cgatacgcga gcgccccaag gccgtgcggc tgaccgaggc
     3961 cgcggccgcg cgcatccgcg agatcatggc gggcgccaaa ggccgttatc agggcgtgcg
     4021 cgtaggcgtc accaacggcg gctgcgccgg catggcctac accatggact atgccgagag
     4081 cgcccagccg ctggaggaag tggtggaaga caagggcgtg aagatattca tcgaccccaa
     4141 agctatcctg ttcctgatcg gcaccgaaat ggatttcgtg cgcgagaaac tcggcgcgcg
     4201 cttcgtcttc aacaatccga accagaccgc ggcctgcggc tgcggcgaat ccgtgtcgat
     4261 cgcgccggcc aaggcggatt aattcctgcc gcggccgccg atttgcagca ggagcgccgc
     4321 ccccgccatg acggcgccat agacgcaaag ccaattccag ttgctcagat gatagataaa
     4381 catcgcgccg gcggacccga tgccgccgcc gatgaacatg ccggttgtga agacggtgtt
     4441 gaggcggctg cgcgccgccg gttgcagcgc atagatgata tgttggttcg agatcatcga
     4501 actctgcacg ccgaaatcga gcagcacgac gccgagaacg aggccggcga cc
//
LOCUS       DASWPU010000185         4326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_86930,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000185 DASWPU010000000
VERSION     DASWPU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86930"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..2294)
                     /gene="lptD"
                     /locus_tag="VGC27_04165"
     CDS             complement(129..2294)
                     /gene="lptD"
                     /locus_tag="VGC27_04165"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY0281804.1"
                     /translation="MTTLALGLSALAPFCLPPPAAAEALKPTGDVLLQADEAIYYPDR
                     KVVVAKGNVEIEYDGRILLADSVTYDQSTDEVTADGHVSLLDKSGNVAFAKQVKLSDR
                     MRDGALQGFGAWIGENGRLVAASAQRSEGRFTTARNVAYTPCKICNQPGRRTPVWQIK
                     ASRVVHDEEKRKIQFKDATVAFFGVPLLYTPYLTKPDPSVRYASGLLTPDIGSSSSTG
                     YFLRLPYYYSISPSQDATIEPLITTRGGNLLLGEYRQRWNDGGMWLQGSVGENPNGGF
                     GGERFQAYTHFFGSGRFQLSPSWQAGFDAQLTSNETYLKRYNISQLDRLVNDVFAVGE
                     YGRSRFAVTGYFFQGLRASDDNRTFPVALPLVEYTYIPLRKWFGGQFRMDVNGVALSR
                     DIGANNQRTTAEARWRYPLVASDGELLTLQLNLRGDLYHTDTPAPLPSDSHYITRGLP
                     YAALDWRWPFIASGKSGRSYIVEPIAQVVVSPYGGNPVNIPNEDSLNVEIDDNNIFSF
                     DQVPGRDLVETGPRANFGVRAESRFSFGYIEGLVGQTFRLKSDPIFADDTGMTGKASD
                     VVGRFSIKFPPYLDLTHRIAVDAEAGTVRRNEVYLTGVYGRSSTQISYIQLAPTLGLP
                     AREEVNTQMDVNFYRNWQGFAAIRRDLISDQMLDTEFGIGYEDECLGISVAYRRKYTS
                     DRDLPPSTSFILRINLKTADTPIQPFSLFPQDVFSYTHP"
     gene            complement(2315..3406)
                     /gene="lptG"
                     /locus_tag="VGC27_04170"
     CDS             complement(2315..3406)
                     /gene="lptG"
                     /locus_tag="VGC27_04170"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY0281805.1"
                     /translation="MSWSWTLYRYLARHFLLGVAMILAAFLLLAFSIDVVDLINRTAG
                     RGVTSGIIIGMALLQLPNLGQKLLPFAVLLGGVFCFVRLSRSQELVATRAAGVSAWDF
                     LVPPLAVAVAIGVIHVLAVTPVSSRMLAQFAALEAKYIKGEASQLSVSANGLWLRQGD
                     ENQQSVVHALTVSEQGVRLDNVIVFLYGPHDRIEGRIDAKSAQLSHGAWIMDDAWVSG
                     ADGHVTHHDRYQLKTTLTPAQIQESFASPDTLSFWALPRFIRAAQNAGFSAVRYKLYF
                     YTLLTMPALFAAMVFMAASFSLKLSRSSGLGRVVLISALSGFGVYFFSDVTRALGQSS
                     ILPVALAATAPAAAAILIGMTLVFHQEDG"
     gene            complement(3403..>4326)
                     /locus_tag="VGC27_04175"
     CDS             complement(3403..>4326)
                     /locus_tag="VGC27_04175"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY0281806.1"
                     /translation="AFFFGALYSLSRLNSDSELVVMASAGFSRAQLSVPMFVAAGIVM
                     ALTYLCGLYLMPAGQRAMTDKVIDIRADIGAALLNEGTFNTPAKGLTVFIREIDADGR
                     IRGVLAHDNRNRNAPVTYLAESGQMAQTPDGARLIMVDGTVEQSAKGGAHLTVLKFER
                     YVFDLDQFTGPARVTGRAASERYLGELFWPDQNLKPSIRAAYFAEAHNRLSQPLYCIA
                     FALIALAAVTRGRRARGANALRMTLASGAATGIRIAGYGVQGLAVSSPAICVLFYLIP
                     LLGAGAALAILASGMPKTVASGPAPAPEGIA"
BASE COUNT          856 a         1294 c         1422 g          754 t
ORIGIN      
        1 gatcacgacg acagcaaagg ctgaacgggt cttaggcagc attaccaagg caagttccca
       61 attacgaagc cccgccgcga tggctcaccc ttaaccggca gtgtagaata agcaaattcc
      121 gttttcgttc aaggatgggt ataggaaaac acgtcttgag gaaacaggct gaaaggctgg
      181 atcggcgtat ccgccgtctt caaattgatc cgcaggatga acgaagttga gggcggcagg
      241 tcgcggtcgc tggtgtattt ccgacggtag gcgacggaaa tgcccaggca ctcgtcctcg
      301 tagccgatgc cgaactccgt atcgagcatc tggtcggaga tcaaatcgcg gcgaatggcc
      361 gcgaaccctt gccaattccg gtagaaattg acgtccatct gggtattgac ctcctcgcgc
      421 gcaggcagac ccagggtcgg ggcgagttgg atatagctga tctgggtgct ggaccggccg
      481 taaaccccag taaggtaaac ttcgtttctt cgcaccgtac ccgcttccgc gtcgacggcg
      541 atacgatggg tgaggtccaa atatggcgga aacttaatgc tgaagcggcc gacgacatcg
      601 gacgccttcc ctgtcatccc cgtgtcgtcg gcaaagatcg ggtcggattt caaccggaag
      661 gtttggccca ccagcccctc aatgtaaccg aaggaaaagc gcgattccgc acgcacgccg
      721 aaattggccc gcggtccggt ctcgaccaga tcgcgaccgg gcacctgatc gaaactgaat
      781 atgttgttgt cgtcgatttc gacgttgagg ctgtcctcgt tgggaatgtt gactggatta
      841 cccccatagg gcgacacgac gacctgggcg atgggttcca cgatgtacga tctgccgctc
      901 ttgcccgagg cgatgaacgg ccagcgccag tcgagcgccg cataaggcag cccgcgggtg
      961 atgtaatgac tgtccgaggg caatggcgcg ggcgtgtcgg tatgatagag gtcgccgcgc
     1021 aggttgagct gcagtgtcaa cagttcgcca tcgctcgcca ccaaggggta acgccagcgc
     1081 gcttcggcgg tggtgcgctg gttgttggcg cctatgtcgc gcgacagggc cacgccattc
     1141 acgtccatcc ggaattggcc gccgaaccat ttgcgcaacg ggatgtaggt atattcgacg
     1201 agcggcagcg cgaccggaaa cgtccggttg tcgtcggagg cgcgcaggcc ctggaaaaaa
     1261 tagccggtga cggcgaaacg gctgcggccg tattcgccca cggcgaagac atcgttcacc
     1321 aaacgatcga gctgcgagat gttgtagcgc ttcagatacg tctcgttcga cgtcaactgg
     1381 gcatcaaagc ctgcctgcca ggaaggcgaa agctggaagc ggccggatcc gaagaagtgt
     1441 gtgtaggcct ggaagcgctc gccgccgaaa ccaccgttgg gattctctcc aacgctgcct
     1501 tgcagccaca tgccgccgtc gttccagcgc tgccggtatt cgccaagaag caagttgccg
     1561 ccgcgagtcg tgatcagcgg ttcgatcgtg gcgtcctgcg agggcgagat ggaatagtag
     1621 tagggcaggc gcaggaagta gccggtggag ctcgacgaac cgatgtcggg cgtcaacagg
     1681 ccgctggcgt aacgtacgct tggatcgggt ttggtgagat acggcgtata gagcaggggc
     1741 acgccgaaga aggcgaccgt cgcgtctttg aactgaatct tgcgcttctc ttcgtcatgc
     1801 accacgcgag aggccttgat ctgccacacc ggcgtgcgcc ggccgggctg gttgcagatc
     1861 ttgcagggcg tataggcgac gtttcgcgcc gtggtgaagc gcccctcgct gcgctgcgcg
     1921 ctcgccgcca ccagtcttcc attctcgccg atccacgctc cgaagccctg aagcgcgccg
     1981 tcacgcatgc gatcgctgag cttgacctgt ttggcgaagg caacattgcc gcttttgtcg
     2041 agcaggctga catgaccgtc ggccgtcacc tcgtcggtgc tctgatcgta agtgaccgag
     2101 tcggccagca ggatgcgccc gtcgtattcg atttcgacgt tgccctttgc gacgacgacc
     2161 ttcctgtccg ggtaatagat cgcttcgtcg gcctgcagca gcacgtcgcc ggtcggcttg
     2221 agggcctcgg ctgcggcggg cggcggcaga cagaaagggg ccagcgccga gagcccgagc
     2281 gccagcgttg tcatccgcga tctcgtccgc ttcttcatcc gtcctcctga tggaagacca
     2341 gcgtcatgcc gatcaggatg gccgccgccg ccggcgccgt ggcggccagt gccaccggga
     2401 gaatgctcga ttggccgagc gcgcgcgtca cgtcgctgaa gaaataaacg ccgaagccgg
     2461 aaagcgcgct gatcaagacc acgcggccga ggccgctgct gcgcgagagt ttcagggaaa
     2521 agctcgccgc catgaacacc atcgcggcga acagcgccgg catggtcagc agcgtgtaga
     2581 aatagagttt gtagcgcacg gccgagaagc ctgcattctg cgcggcgcgg atgaaccgcg
     2641 gcagcgccca gaacgatagc gtatcgggcg acgcgaaact ctcttgaatc tgggcgggtg
     2701 tcagcgtggt tttgagttgg tagcggtcgt gatgggtcac atgaccgtcc gctccgctca
     2761 cccaggcgtc atccatgatc caggcgccat gggacagttg cgcgcttttg gcgtcgatgc
     2821 ggccctctat gcgatcatgc gggccataga gaaagacgat gacgttatcc aggcgcaccc
     2881 cctgctcgga gacggtgagg gcgtgaacga cagactgctg gttctcgtcg ccctgcctca
     2941 accacagacc gtttgctgaa accgaaagct gcgaagcctc gcctttgatg tacttcgctt
     3001 ccagcgcggc gaattgggcg agcatgcgcg aggaaaccgg ggttacggcc agcacatgga
     3061 tcacgccgat cgcgacggcg accgccagcg gcggcaccag gaaatcccag gccgatacgc
     3121 cggccgcgcg cgtcgcgacc aactcctggc ttcgcgaaag ccgcacgaaa caaaagaccc
     3181 cgcccaacag aaccgcgaac ggcagcagct tctgtccgag attgggaagc tgcaacagcg
     3241 ccatgccgat gataatgccg ctcgtgaccc cgcgcccggc cgtgcggttg atgagatcga
     3301 cgacatcgat cgaaaaggcg agcagcagga acgcggctaa gatcatcgcc acgccgagca
     3361 gaaagtggcg cgccagatag cggtacagag tccacgacca gctcacgcaa taccctccgg
     3421 agctggggcg ggcccgctcg cgacggtctt gggcatgccg ctggcgagta tggccagggc
     3481 ggcgcccgct ccgagcaagg ggatcaggta gaacagcacg cagatcgcgg ggctgctcac
     3541 ggccaagcct tgcacgccgt agcccgcgat cctgatgccg gtggcggcgc ccgaggcgag
     3601 ggtcatgcgc aaggcgttgg cgccgcgcgc gcgtcgcccg cgggtaacgg cggccagggc
     3661 gatcagcgcg aaagcgatgc aatagagcgg ctgcgagagc cgattatggg cttccgcaaa
     3721 gtaagcggcg cggatgctcg gcttgaggtt ctgatcaggc cagaaaagct cgccgaggta
     3781 acgctcgctg gcggcgcggc cggtgacgcg ggcggggccg gtaaactgat cgaggtcgaa
     3841 aacgtagcgc tcgaacttga gcaccgtcag atgcgccccg cccttggcgc tttgttcgac
     3901 ggtgccatcg accatgatga ggcgcgcgcc gtcgggggtc tgcgccatct gtccgctttc
     3961 cgccagatag gtgaccggcg cgttccggtt gcggttgtcg tgcgccagca cgccgcggat
     4021 gcgtccgtcg gcgtcgatct cgcggatgaa gacggtgaga cccttggcgg gggtgttgaa
     4081 ggtgccttcg ttgagcagcg ccgcgccgat gtcggcgcgg atgtcgatca ccttgtccgt
     4141 catggcgcgc tggcccgcgg gcatcagata aaggccgcac aaataggtca acgccatgac
     4201 gattccggct gcgacgaaca tcggcacgga gagctgggca cggctgaagc cggccgacgc
     4261 catcaccacc agttcgctgt ccgaattgag cctggacaag gaatagagcg cgccgaagaa
     4321 gaaggc
//
LOCUS       DASWPU010000186         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_62844,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000186 DASWPU010000000
VERSION     DASWPU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62844"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VGC27_04180"
     CDS             <1..601
                     /locus_tag="VGC27_04180"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY0281807.1"
                     /translation="KRPGIYYINLRDTAEVPDWTLPTLTYHEAIPGHHLQGTIAQESP
                     LPLIRKISGYNAYVEGWALYAEQLAVEAGLYADDPWGHIGQLHDAQLRAVRLVVDTGL
                     HAMRWSREQAIKYYADTLGDPVSGATTEVERYCVWPGQACGYMIGKLTLLRLRDKAKA
                     ALGPRFDLRKFHDAVLLSGALPLAVLEKVVDGYIARSKA"
     gene            complement(608..1045)
                     /locus_tag="VGC27_04185"
     CDS             complement(608..1045)
                     /locus_tag="VGC27_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0281808.1"
                     /translation="MQVIEILREKGREVVSLPGDATLSEATRLLAEKHIGALVVLEKN
                     GALAGILSERDVVRAAAREGVPALARPASAYMTREVATCAETDTVEELMEMMTQGRFR
                     HVPVLDGDKQLCGIVSIGDVVKMHIEETMREAATLRQYITAAG"
     gene            1269..1362
                     /locus_tag="VGC27_04190"
     tRNA            1269..1362
                     /locus_tag="VGC27_04190"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Ser,seq:gct)
     gene            complement(1564..1809)
                     /locus_tag="VGC27_04195"
     CDS             complement(1564..1809)
                     /locus_tag="VGC27_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281809.1"
                     /translation="MIDDGRAAAGLPKELQLQDFRRTAQTEAGAGGATLDEIRALARH
                     TTRQAGEHYVVPDSRFVDAAQEKRRALRKAKTGTTTK"
BASE COUNT          362 a          610 c          605 g          391 t
ORIGIN      
        1 caaacggccc ggcatctatt acatcaacct gcgcgacacc gccgaagtgc cggactggac
       61 gctgcccacg ctcacctatc acgaagcgat ccccggccat catctgcaag gcaccatcgc
      121 gcaggagtcg ccgctgccgc tgatccgcaa gatttcggga tacaacgcct atgtcgaagg
      181 ctgggcgctc tatgccgaac agcttgcggt ggaagccggt ctttatgccg acgatccctg
      241 gggccatatc ggccagcttc acgacgcaca attgcgcgcc gtgcgtctgg tggtggacac
      301 cggtttgcat gcgatgcgct ggagccgcga acaagcgatc aaatattatg ccgacacgct
      361 gggcgacccg gtgagcggag ccaccacgga ggtggaacgc tattgcgtct ggcccggcca
      421 ggcctgcggc tacatgatcg gcaagctgac gctgctgcgc ctgcgcgaca aagccaaggc
      481 ggcgctgggg ccgcgtttcg acctgcgcaa attccacgac gcggtgctgc tctccggcgc
      541 gctgccgctc gcggtgctgg aaaaagtcgt ggacggctat atcgcccgaa gcaaggctta
      601 aggttcttca gcccgccgcg gtgatgtatt ggcgcagcgt ggccgcctcg cgcatcgttt
      661 cctcgatgtg catcttgacc acgtcgccga tcgagacgat gccgcagagc tgtttatctc
      721 cgtcgaggac ggggacatgg cggaaacggc cttgggtcat catttccatc agttcttcga
      781 cggtgtcggt ttcggcgcaa gtcgccactt cgcgcgtcat gtaggcgctt gccggccgcg
      841 ccaaggcggg cacgccttcc cgcgccgccg cgcgtacgac atcgcgttcg gacagaatgc
      901 cggcgagcgc gccgttcttt tccagcacca ccagggcgcc gatgtgtttt tccgccagca
      961 ggcgcgtggc ctccgaaagc gtggcgtcgc cgggcagaga caccacctcg cgcccttttt
     1021 cacggagaat ctctatgacc tgcatgtgcc tctctcctgc acgtttcgga cgaggtccgg
     1081 cgggtgttcc ccgcttgcga gaaaggctgc gccagttgcg agcttcttgc aatagtggag
     1141 tccagaacat ggttatcctc ctgttggacc taaactttcg gtatttgggg aaggaggcgg
     1201 gcttgaggca ggctacaatc tggggcaaga gtggcctttg ggcgggcccg cccgaatggg
     1261 ccgcgtgcgg agctgtggcc gagaggctga aggcggcggt ttgctaaacc gttatacggc
     1321 tgaaaaaccg tatcgagggt tcgaatccct ccggctccgc caattacttg attttgctga
     1381 gaaaatttat actgaagcgg tccagatgga gcgaacatta actcgcttat ttcaacattt
     1441 cgttccgctg catttcaaca tttaccccct cttcgttctg taggtgaaac gccctggcgg
     1501 gagctgctaa gtacgcggca gaacgaatcg ccgctcatac ggaggtcgag cattgcgctg
     1561 tccctatttc gtcgtcgtgc cagtcttcgc cttacgaagg gcgcgccgct tctcctgagc
     1621 cgcgtcgacg aagcgggaat ccggcacgac gtagtgttca ccagcttgcc gcgtcgtgtg
     1681 acgggccagc gcacgaattt catcgagcgt cgccccgcca gcaccggctt cggtctgggc
     1741 ggtacgacgg aagtcctgga gctgcaactc cttcggtagc cctgcggcgg cgcggccgtc
     1801 atcgatcatg acctcaacgg caatctgacg acggaccaaa gctcgtatta tcagtacgat
     1861 cccgagaacc ggttgatgaa ggcgacgacc gccgccatga cggcggccta tgcctacgat
     1921 ccgctagggc ggcggaccac gaaaaccgtc acaggcggga cctatgcg
//
LOCUS       DASWPU010000187         1779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_38551,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000187 DASWPU010000000
VERSION     DASWPU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38551"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VGC27_04200"
     CDS             <1..432
                     /locus_tag="VGC27_04200"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEY0281810.1"
                     /translation="LGVEPLSDAFDAAYLAEALKGKKTPIKSALLDQRVIAGLGNIYV
                     CEALFRAGISPKRLAGKIQIASIAPLVTSIKTVLKDAIKAGGSSLRDYKNADGELGYF
                     QHHFAVYDRAGEPCPKKGCGGTVKRIVQSGRSTFYCPKCQK"
     gene            436..861
                     /gene="arfB"
                     /locus_tag="VGC27_04205"
     CDS             436..861
                     /gene="arfB"
                     /locus_tag="VGC27_04205"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY0281811.1"
                     /translation="MGKIRVTANIALNESELEETFVLASGPGGQNVNKVSSAVQLRFN
                     AAQSPSLPEPVRFRLMTIAGGKLTKDGVLVLTGRQFRDQARNRADVRERLVELIRTAA
                     VIPKKRRRTKPPRSAKENRLQHKKKHAELKRDRAKPRGD"
     gene            879..1655
                     /locus_tag="VGC27_04210"
     CDS             879..1655
                     /locus_tag="VGC27_04210"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY0281812.1"
                     /translation="MEPKNILVRAEGAVGVVTLNRPKALNALTAQLLDELATALEAFD
                     ADPAIRAIVVTGSERAFAAGADIKEMAQKSYMDMFKADFFADPCNRIAAIRKPVIAAV
                     AGYALGGGCELAMICDFIIAADTAKFGQPEITLGVIPGMGGSQRLTRFVGKSKAMDMC
                     LTGRMMDAAEAERAGLVSRVVPATDLLAEAMKAAAKIAEQSLPVLMMAKEAVNRAYET
                     TLAEGIRYERRLFHAAFATEDQKEGMAAFTEKRKPSFKDN"
BASE COUNT          382 a          563 c          549 g          285 t
ORIGIN      
        1 ctgggcgtcg agccgctatc cgatgccttc gatgcggcct acttggcgga ggccctgaaa
       61 gggaagaaga caccgatcaa gtcggcgctg ctcgaccagc gggtgatcgc ggggcttggc
      121 aacatctacg tttgcgaggc gctgttccgt gcgggcatct caccgaagcg attggccggg
      181 aagattcaaa tagcctccat cgcgccactg gtcacttcca tcaagaccgt gctgaaggac
      241 gccatcaaag ccggcggctc gtccctgcgc gactataaga acgccgacgg cgaattgggc
      301 tatttccagc atcatttcgc cgtttacgac cgcgccggcg agccgtgccc gaaaaagggc
      361 tgcggcggaa ccgtaaaacg catcgtgcag agcggccgct cgaccttcta ttgtccgaag
      421 tgccagaagt aagtcatggg caagattcgc gtcactgcaa acatcgcgct caatgaaagc
      481 gagcttgaag agacattcgt gctggcatcg ggaccaggcg gccagaacgt caacaaggtt
      541 tccagcgccg tgcagttgcg cttcaacgcg gcgcagtccc cttcgctgcc ggagccggtg
      601 cgtttccggc tgatgaccat cgctggcggg aagttgacca aggacggcgt gctggtgctg
      661 acggggcgac aattccgcga tcaggcgcgc aaccgcgccg atgtgcgcga gcggctggtg
      721 gaactcatcc gcaccgccgc ggtgataccc aaaaagcggc gccgcaccaa gccgccacgc
      781 tcggccaagg aaaatagatt gcagcacaaa aagaaacatg cggaactgaa acgcgatcgc
      841 gccaagccgc gcggcgactg atctcgaaag aacctccgat ggaaccgaaa aacattctgg
      901 tgagggccga aggcgccgtc ggcgtcgtca ctctcaaccg gcccaaggcg ttgaacgcgc
      961 tgaccgccca attgctagac gaattggcga cagcattgga agcgttcgac gccgatcccg
     1021 ccatacgcgc aattgtcgtt accggctccg agcgtgcctt cgccgcgggg gccgacatca
     1081 aggaaatggc ccagaaatcc tacatggaca tgttcaaggc cgactttttt gccgacccct
     1141 gcaaccgtat cgccgccatc cgcaagccgg tcatcgccgc agttgcgggc tatgcgctgg
     1201 gtggcggctg tgagctggcc atgatctgcg atttcatcat cgcggccgac accgccaagt
     1261 tcggccagcc ggaaatcacg ctcggcgtca tccccggcat gggcggcagc cagaggctca
     1321 cccgcttcgt cggcaagtcc aaggcgatgg acatgtgcct gaccggacgg atgatggacg
     1381 cggccgaggc ggagcgcgcc ggtcttgtca gccgcgtggt gcccgccaca gaccttctgg
     1441 ctgaggcgat gaaggcggcg gccaaaatcg ccgaacagtc cctgcccgtg ctgatgatgg
     1501 ccaaggaagc cgtaaaccgc gcttacgaga ccaccctggc ggagggtatc cgctacgagc
     1561 ggcggctgtt ccatgccgcg ttcgcaaccg aagaccagaa ggaaggcatg gccgccttta
     1621 cggagaagcg gaagccgtca ttcaaagaca actaacccag gatttgaccc cggcgcgggc
     1681 gcgggctata cgccccggct ccaaacggaa ttgcaggatc agccatgccc aatatcgcct
     1741 ccgccaaaaa gcgcgtgcgc acctcggcca aacgcgccc
//
LOCUS       DASWPU010000188         3467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_81027,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000188 DASWPU010000000
VERSION     DASWPU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81027"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="VGC27_04215"
     CDS             complement(<1..1671)
                     /locus_tag="VGC27_04215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY0281813.1"
                     /translation="MNFIMSHLLSILTFLPLAGALFILATRGSEKTVASTARWVALIV
                     TLADFALSLLLWANFDGSTASFQFVERAAWLGHGISYHMGVDGISMLFVVLTGGLMPL
                     CILASWKSVQTRVAEYMIAFLVLETLMMGVFAALDLFLFYVFFEGGLIPMFLIIGVWG
                     GKRRVYASFKFFLYTLLGSLFMLLAILAMFFTAGTADIETLIETAHFPVAMQNLLWLG
                     FFASFAVKLPMWPVHTWLPDAHVEAPTAGSVILAAILLKMGGYGFLRFSLPMFASASV
                     TFAPLIFVLSIIAIIYTSFVALAQEDVKKLIAYSSVAHMGFVTMGLFTLTQVGIDGGI
                     FQMLSHGVVSGALFLSVGVIYDRMHTREISAYGGLVNRMPIYAACFMVFALANIGLPG
                     TSGFVGEFLTLLGAFRVNSWIAILAATGVILSACYMLYLYWRVIFGELVKPALKTIKD
                     LSAREVALLAPLAVIIIALGVYPKPVFDVTHASVAHLIAQHKAAVAYDERGPNAPQIE
                     MKYTSLSSWPPLRRPSSNESRRDAEVAEALFHSAFSASSRANLDDRVKP"
     gene            complement(1668..>3467)
                     /gene="nuoL"
                     /locus_tag="VGC27_04220"
     CDS             complement(1668..>3467)
                     /gene="nuoL"
                     /locus_tag="VGC27_04220"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY0281814.1"
                     /translation="LGHSQIVTPGQWIVSGDFEADWTIRVDALTAVMLVVVTGVSSLV
                     HLYSIGYMDEDESRPRFFSYLSLFTFAMLMLVTANNFLQMYFGWEGVGLASYLLIGFW
                     YKRPSANAAALKAFVVNRVGDFGFALGIIAIFTVFKTLDFDAVFAAAPAMAGKSFVFA
                     GYNVDILTAICLLLFMGAMGKSAQLGLHTWLPDAMEGPTPVSALIHAATMVTAGVFLV
                     CRCSPLYELAPVALGFVAVIGASTAFFAASVGLFQNDIKRVIAYSTCSQLGYMFAAAG
                     VSAYGAAMFHLFTHAFFKALLFLAAGSVIRAVHHEQDMRKMGGLAKAIPFTWAMMLIG
                     NLALTGFGFPVLDIGTAGFYSKDGIINAAYLAGTPIATYAFWLTVIAALMTSFYSWRQ
                     FLMTFHGKFRGGEGHHGVKLKDVHESPLTMLVPLGVLAVGALFAGAIFAHLYIGADSG
                     SFWRGAVAAAYGHAGAEELPLWVELAPFAVTLIGFGIAWYYYILKPALPAQLAAKKGL
                     VYTFVYNKWYFDELYAFLFVRPALRIGRFLWKSGDGATIDGLGPDGIAARVLDAARGA
                     VKLQSGYVYHYAFAMLIGVVALTTWFVVMGGAP"
BASE COUNT          776 a         1100 c         1098 g          493 t
ORIGIN      
        1 gggcttgacc cggtcatcca ggttcgcacg cgaagacgcg gagaacgcgg agtggaatag
       61 cgcctccgcg acctccgcgt ctctgcgtga ttcgttgctg gatggccgcc tcaagggtgg
      121 ccatgacgaa agggaggtgt acttcatttc aatttgcggt gcgttcggtc ccctctcgtc
      181 ataagccacg gccgccttgt gctgggcgat cagatgcgcc accgatgcgt gcgtgacgtc
      241 gaacaccggc ttgggataaa cgcccagcgc gatgatgatg accgccagcg gcgccagcag
      301 cgccacttcg cgcgccgaca ggtccttgat cgtcttgagc gccggtttca ccagttcgcc
      361 gaagatcacc cgccaataga gatagagcat gtagcaggcc gagaggatca cgccggtggc
      421 cgcgagaatc gcaatccaac tgttgacgcg gaacgcgcct agcagagtca gaaactcgcc
      481 gacgaagccc gaggtgccgg gcagcccgat attggcgagc gcaaagacca tgaaacaggc
      541 cgcatagatc ggcatgcggt tgaccaggcc gccgtaagcg gaaatctcgc gcgtatgcat
      601 gcggtcgtag atcacgccga cggagaggaa cagcgcgccc gaaaccacgc cgtggctcaa
      661 catctggaag atgccgccgt cgatccccac ctgcgtcagg gtgaacaggc ccatggtgac
      721 gaagcccata tgggcgaccg aggaataggc gatcagcttc ttcacgtcct cctgggccag
      781 cgccacgaag gaggtgtaga tgatcgcgat aatcgacagc acgaagatca gcggcgcgaa
      841 ggtcaccgag gccgaggcga acatcggcag ggaaaagcgc aggaagccgt agccgcccat
      901 cttgagcagg atcgccgcca ggatgacgga gccggccgtc ggcgcctcga catgggcgtc
      961 gggcagccag gtatgcaccg gccacatcgg cagcttcacc gcgaaggagg cgaagaagcc
     1021 gagccacaac aggttctgca tcgccaccgg gaaatgcgcg gtctcgatca gcgtttcgat
     1081 gtcggcggtg ccggcggtga agaacatcgc caaaatcgcc agcagcatga acagcgagcc
     1141 gagcagcgtg tagaggaaga acttgaagct ggcataaacg cggcgcttgc cgccccacac
     1201 cccgatgatc aggaacatcg ggatcagccc gccttcgaag aagacgtaga acaggaacag
     1261 atcgagcgcc gcgaacacgc ccatcatcag ggtttcgagc accaggaagg cgatcatgta
     1321 ttccgccacc cgcgtctgaa ccgacttcca gctcgccagg atgcagagcg gcatcaaccc
     1381 accggtcaag acgacgaaca gcatcgaaat gccgtccacg cccatgtggt agctgatccc
     1441 gtggcccagc cacgccgcgc gctccacgaa ctggaaggaa gccgtcgagc cgtcgaaatt
     1501 cgcccacagc aggagcgaga gcgcgaagtc cgccagcgtc acgatcagcg ccacccagcg
     1561 cgccgtggac gccaccgtct tctccgaacc gcgcgtcgcc aggatgaaca gcgcgcccgc
     1621 cagcggcagg aaggtgagga tgctgagcag gtggctcatg atgaagttca tggcgcgcct
     1681 cccatcacca cgaaccaagt cgtcagcgcc accacgccga tcagcatggc gaaggcgtag
     1741 tgatagacgt agcccgattg cagcttgacc gcgccgcgcg cggcatcgag cacgcgggcg
     1801 gcgatgccgt caggccccag cccgtcgatg gtcgccccgt cgccggactt ccacaggaag
     1861 cgcccgatcc gcagcgcggg gcgcacgaac agaaaggcgt aaagctcgtc gaaataccat
     1921 ttgttgtaga cgaaggtgta gacgaggccc ttcttcgcgg ccagctgcgc cggaagcgcc
     1981 ggcttgagga tgtagtagta ccaggcgatg ccaaagccga tcagggtgac ggcgaacggc
     2041 gccagttcca cccatagcgg cagttcctcc gcgccggcgt ggccataagc cgccgccacc
     2101 gcgccgcgcc agaagctgcc ggaatccgcg ccgatataaa gatgcgcgaa gatcgccccg
     2161 gcaaacaacg cgcccaccgc cagcaccccc agcggcacca gcatggtcag cggggattcg
     2221 tgcacgtcct ttagcttgac gccgtgatgg ccttcgccgc cgcggaactt gccgtggaag
     2281 gtcatcaaga attgccgcca ggaatagaac gaggtcatca gcgcggcgat caccgtcagc
     2341 cagaaggcat aggtcgcaat cggcgtgccc gccagatagg ccgcgttgat gatgccgtcc
     2401 ttggaataga aacccgccgt gccgatgtcg aggaccggga agccgaagcc ggtcagcgcc
     2461 agattgccga tcagcatcat cgcccaggtg aagggaatgg ctttcgccag cccgcccatc
     2521 ttgcgcatgt cttgttcgtg atgcacggcg cggatgaccg agccggcggc caggaacagc
     2581 agcgccttga agaaggcgtg ggtgaagagg tggaacatcg ccgcgccgta ggccgagacg
     2641 cccgccgccg cgaacatgta gccgagctgc gaacaggtcg aataggcgat cacgcgcttg
     2701 atgtcgttct ggaacaggcc gaccgaggcg gcgaagaacg ccgtggacgc tccgataacg
     2761 gccacgaagc cgagcgccac cggcgccagt tcgtacaagg gcgagcagcg gcagaccagg
     2821 aacacgcccg ccgtcaccat ggtcgcggca tggatcaagg cggagaccgg ggttgggcct
     2881 tccatcgcgt cgggtagcca ggtgtgcagg ccaagctggg ccgacttgcc catcgcgccc
     2941 atgaacagca acaggcaaat cgcggtcagg atatcgacat tatatccggc gaagacgaag
     3001 ctttttccgg ccatagcggg cgcggcggcg aacaccgcgt cgaaatccag cgtcttgaag
     3061 accgtgaaaa tggcgatgat gcccagtgcg aaaccgaaat cgccgacccg gttgacgacg
     3121 aaggccttca acgccgccgc gttggcgctc ggccgcttgt accagaaccc gatcaacaga
     3181 taggacgcca gcccgacgcc ttcccagccg aaatacatct ggaggaaatt gttcgccgtc
     3241 accagcatca gcatcgcgaa ggtgaacagc gagagatacg agaagaaccg agggcggctt
     3301 tcgtcttcgt ccatgtagcc gatggagtag aggtgcacca gcgaggaaac gcccgtcacc
     3361 accaccagca tcaccgccgt cagcgcgtcg acgcggatcg tccagtccgc ctcgaagtcg
     3421 ccggaaacga tccattgccc cggcgtgacg atctgcgaat gcccgag
//
LOCUS       DASWPU010000189         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_86941,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000189 DASWPU010000000
VERSION     DASWPU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86941"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1457)
                     /locus_tag="VGC27_04225"
     CDS             complement(162..1457)
                     /locus_tag="VGC27_04225"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0281815.1"
                     /translation="MGGVAAVTRQTDDEFEVGETLPISVQQSGIPIFECKATVCRSEK
                     NVFGAKIAFKFLDGYIEFDKLLNRNLHAQILTRASLVGPDAAQLVPREYRTLCADALN
                     VLRAYRSILSPDAARAPELATRIDPQAAFEACEDRLMQHWRSLWRTANDIVRTVMDDR
                     ESREATKVFTELVLTPEMRMGAIWDRSYAKPLGYPGDFEIMNQVYDWNRVGNTAYEML
                     LHRIGLDVAECIKTRMEIVRDHIKDTSRRDLGRPARILSLGSGPAREVELFLAGSGAQ
                     SGRAEFTLIDQEQLALRYAHEKAYPHTLKSQGRYSVRALNISFTDILRGISAMNSLPQ
                     QDMIYSVGLLDYLTDRRATGIVRRLYDMLAPGGLLIIGNMNETPLSNLWPMEFITDWT
                     LYYRNEAQMLAWADGLNTVRIWTETDPTGRVRLLYMLKP"
BASE COUNT          327 a          513 c          525 g          373 t
ORIGIN      
        1 tcgggcttcc ttacgccaaa cagctcgtcg aactgcacga cggaacgctc cgtatctcaa
       61 gcgaactggg caagggaaca accgtcacga tttccctgcc gcgcagccgg ctgttggcgc
      121 gcggcgcccc gctactcatt aaagaggcag tgtaaggttt ctcagggctt cagcatgtag
      181 agcagacgaa cgcgtccggt cgggtcggtt tcggtccaga tcctgaccgt gttgagaccg
      241 tcagcccaag ccagcatctg agcttcgttc cggtagtaca atgtccaatc ggtgatgaac
      301 tccatcggcc aaagattgct caatggcgtc tcgttcatgt tgccgatgat gaggaggccg
      361 cccggcgcga gcatgtcgta aaggcgccgc acgatgcccg tggcgcgtct gtccgtgaga
      421 tagtcgagca accccacgga atagatcatg tcctgttgcg gcagcgagtt catggcgctt
      481 atgccgcgca agatatccgt gaaggatatg ttgagcgcgc gcacgctgta gcgtccctgc
      541 gatttgagcg tatgcgggta cgccttctcg tgggcgtagc gcagggccaa ttgctcctgg
      601 tcgatcagcg tgaactcggc cctgccgctt tgcgcgccgc tgccggcgag gaacaattcc
      661 acttcgcgcg ccggtccgct tccgaggctc aatatgcggg cagggcgccc gagatcgcgc
      721 cgtgaagtat cctttatgtg atcgcgcacg atctccatgc gcgtctttat gcattcggcg
      781 acgtcaagac cgatacggtg aaggagcatt tcgtaggccg tgtttccgac gcggttccag
      841 tcgtagacct ggttcatgat ctcgaaatcg cccggatagc cgagcggctt ggcatagctt
      901 cgatcccaaa tcgcgcccat ccgcatttcg ggcgtcaata cgagttcggt gaacaccttc
      961 gtcgcttcgc ggctttcgcg gtcgtccatg accgtccgca cgatgtcgtt ggcggtgcgc
     1021 cacaaggaac gccaatgctg catgaggcgg tcttcgcagg cttcgaaggc ggcctgggga
     1081 tcgatgcgag tggccagctc cggcgcgcgc gcggcgtcgg ggctcaggat gctgcggtag
     1141 gcgcgcagaa cattcagggc atccgcgcag agcgtgcgat attcccgcgg aaccagctgt
     1201 gcggcatcgg gacccacgag agacgctctg gtgaggattt gcgcatgcag attccgattg
     1261 agcagcttgt cgaattcgat gtagccgtcc agaaacttga acgcgatttt cgcgccgaaa
     1321 acgttcttct cgcttcggca gaccgttgct ttgcactcga agatcgggat gccggactgc
     1381 tgaacgctga tcggcagcgt ctcgccgact tcgaattcgt cgtccgtttg ccgcgtcacc
     1441 gccgcgacgc cgcccaagca gatgttctgg atctgataaa ggttggaact tgcccgaacg
     1501 cgcggtggga tgcccggaaa gagcttttgg gtttcatagc ggggcggccg ataccagatc
     1561 tcgcggcccg aagacccacg caactcatca tacgctaaca ttgttgtcct taaagtttgg
     1621 cccaggcccg gtctttggga cgcttggcgg cttcgcttaa gccttcacgc cagccccaaa
     1681 atcgcaccag ttacccacag gttcgtgctt gaattcccag agttttaatc gttaatag
//
LOCUS       DASWPU010000190         4132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_7715, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000190 DASWPU010000000
VERSION     DASWPU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7715"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VGC27_04230"
     CDS             complement(<1..445)
                     /locus_tag="VGC27_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0281816.1"
                     /translation="MSFSRANRSEFSDWWWTVDRLALAAMLALIAIGLMLAFAASPAA
                     SGGPLTEGDFRYAAKQIAFAVIAACILGVASTLTPAQVKLAALVVFATALIGSFAVAF
                     AGTEISGARRWLNLGWMTLQPSEFLKPGFAVLAAAILTERTPTRLS"
     gene            complement(442..1854)
                     /gene="murD"
                     /locus_tag="VGC27_04235"
     CDS             complement(442..1854)
                     /gene="murD"
                     /locus_tag="VGC27_04235"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY0281817.1"
                     /translation="MIAVRSFADRQVAVFGLGRSGLASVRALKAGGAGVFAWDDKDEL
                     RRAAAAEGAAVLPWRDWPWDGIAALVLSPGVPLTHPRPHDIVVKAKEAGVEVIGDIEL
                     LAREIGPEAPHRAPVIAVTGTNGKSTTTALIGHILAAAGFDAQVGGNIGKPALELSPP
                     RAMTVYVLEVSSYQIDLSPGLRPDVTVLSNITPDHIDRHGSMENYAAVKTRLLQQTAK
                     PGLNVVGVDDPHGAAVFTKLTSNGGPPCVPVSVGKVLGRGVFVLDGVLYDAQSERAAK
                     VMDLSAAEHLPGAHNWQNAALGYAAVKPYAKDARVIASSIANFPGLAHRIEEVGRAGK
                     VRFINDSKATNADAAERALVCFDDIFWIAGGKPKEGGIAPLAPLFSRIRKAYLIGEAA
                     DEFAATLEGKAAYEISGTLENALAAAAADAAQAEASAPVVLLSPACASFDQFRDFEQR
                     GDVFRALVAKLAAQPVREAS"
     gene            complement(1857..2948)
                     /gene="mraY"
                     /locus_tag="VGC27_04240"
     CDS             complement(1857..2948)
                     /gene="mraY"
                     /locus_tag="VGC27_04240"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005297383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY0281818.1"
                     /translation="MFHFLFYLPHADHYAVLRLFKYLTFRSGGAVLTALFVAFVIGPP
                     LINWLKLKQGMGQPIRDDGPKRHVVEKQGTPTMGGLLILVPGLVATLLWADLSNAYVW
                     IVILVTVSYGLLGFADDYLKVTRHSSAGISGKLRLLIEFGVAFLATWLIMQVESPSQS
                     GMLAVPFFKSVLLPLGFAFILVGGVVIAGAANAVNFTDGLDGLAIMPVMIAAGAFVLI
                     TYLVGNERFASYLQLNYVRDTGELAVFLAALIGAGLGFLWYNAPPAMVFMGDTGSLPL
                     GGALGAVAVAVKHEIVLAIVGGLFVLETVSVIVQVISFKLTGKRVFRMAPVHHHFEQL
                     GWKEPTIVIRFWIIAVVLALIGLATLKLR"
     gene            complement(2957..>4132)
                     /gene="murF"
                     /locus_tag="VGC27_04245"
     CDS             complement(2957..>4132)
                     /gene="murF"
                     /locus_tag="VGC27_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY0281819.1"
                     /translation="RPSGGVTPDGAGGPQIAVANTQRALEDLAVASRARIDAKIVAVT
                     GSAGKTTAKEMLKLAFGALGKTHASRASLNNHWGVPLTLASLPREAAFGVVEIGMNHF
                     GEIRPLVSFARPHVALVTTIAPAHLEFFGTCEAIVDAKSEIFEGVVAGGAALIPSDNP
                     YAERLIARAKQSHVARILGFGAKPGSEARLLSYEESGEGARVKADILGQPVDFFLGAP
                     GSHNAANALGALLCVAAAEGDVLNAAAALKKFSALQGRGARFAAGGVEVIDESYNANP
                     ASMAAALSLLGKAPGRRIAVLGDMLEMGPGAPAHHAGLTAALAAARVDLVFLCGPLMQ
                     ALWEVLPSPQRAAYAPNSAALAPDVASALRAGDTVLVKGSNGSRMSVIVEALKARAG"
BASE COUNT          767 a         1368 c         1397 g          600 t
ORIGIN      
        1 tggacaggcg cgtcggggtg cgctcggtca ggatcgcggc cgcaagaacg gcgaaaccgg
       61 gcttcaggaa ctccgacggc tgcaaggtca tccagcccag attgagccag cgccgcgcgc
      121 ccgaaatttc ggttcccgcg aacgccacgg cgaacgaacc gatcagcgcc gtcgcgaaca
      181 cgacgagcgc ggcaagcttg acctgcgccg gcgtcaaagt ggaggcgacg cccaggatac
      241 aggccgcgat gacggcgaac gcgatctgct tggcggcata acggaaatcg ccttcggtca
      301 gcggcccgcc gctggccgcc gggctggcgg cgaaggccag catcaggccg atggcgatca
      361 aggccagcat ggcggcgagc gccagacggt ccaccgtcca ccaccagtcc gagaattcgc
      421 tgcggttggc gcggctgaaa ctcatgacgc ctcccggacc ggctgcgccg caagcttggc
      481 gaccaaggcg cggaagacgt cgcccctctg ctcgaaatcg cgaaactgat cgaacgaggc
      541 gcaggccggc gacagcagaa ccacgggagc ggacgcctcg gcctgcgcgg catcggcggc
      601 ggcggcggca agcgcgtttt ccagcgtgcc ggagatttcg taagcggcct tgccttcgag
      661 ggtggccgcg aattcatccg ccgcctcgcc gatcagataa gccttgcgga tgcgggagaa
      721 aagcggcgcc aaaggtgcga tgccgccctc cttcggtttg ccgccggcga tccagaagat
      781 gtcgtcgaaa cagaccagcg cccgctcggc ggcgtcggcg ttggtggcct tggaatcgtt
      841 gatgaagcgc accttgccgg cgcggccgac ttcttcgatg cggtgggcca gccccggaaa
      901 gttcgcgatg gacgaggcga tcacgcgcgc atccttggcg taaggcttga ccgcggcata
      961 gccaagcgcc gcgttctgcc aattgtgcgc cccgggcaga tgttcggccg cgctcaggtc
     1021 catgaccttc gccgcgcgtt cgctttgcgc gtcgtagagc acgccgtcga gcacgaagac
     1081 gccgcgtccg agcaccttgc cgaccgagac cggcacgcat ggcggcccgc cattggaggt
     1141 gagcttggtg aaaaccgcag cgccatgggg atcgtccacg ccgacgacgt tgagccccgg
     1201 cttcgccgtc tgttgcagca gcctggtctt caccgcggca tagttctcca tcgaaccgtg
     1261 gcggtcgata tggtcgggcg tgatgttgga caggaccgtc acgtcggggc gaaggcccgg
     1321 cgacagatcg atctggtagg aggaaacctc cagcacatag accgtcattg cgcgcggcgg
     1381 cgaaagttcc agcgccggct tgccgatgtt gccgccgacc tgcgcatcga accccgcggc
     1441 cgcgaggatg tggccgatca gcgccgtggt cgtcgatttg ccgttggtgc cggtgacggc
     1501 aatcaccggc gcgcggtgcg gcgcctcggg accgatttcg cgcgccaaga gttcgatgtc
     1561 gccgatcact tcgacgcccg cctctttcgc cttgaccacg atgtcgtggg gcctgggatg
     1621 ggtgagcggc acgccgggac tgagcaccag cgcggcgatc ccgtcccacg gccagtcgcg
     1681 ccagggcaac accgcggcgc cttcggcggc ggcggcccgg cgcagctcgt ccttgtcgtc
     1741 ccaggcgaag accccggcgc cgcccgcttt caatgcgcgc acggacgcca gccccgagcg
     1801 ccccagcccg aaaacggcga cctgcctgtc tgcgaaggag cggaccgcga tcacgtctac
     1861 ctcaacttca gcgtggcaag gccgatcagc gccagcacca ccgcaatgat ccagaagcgg
     1921 atcacgatgg tcggttcctt ccagcccaat tgctcgaaat gatgatggac cggcgccatg
     1981 cgaaagacgc gcttgcccgt cagcttgaac gaaatcacct gcacgatcac cgagacggtt
     2041 tcgagcacga acagcccgcc gacgatggcg agcacgatct cgtgcttgac cgcgaccgcg
     2101 accgcgccga gcgcgccgcc cagcggcagc gagccggtat cgcccatgaa caccatcgcg
     2161 ggcggggcgt tgtaccacag gaagccgagg ccggcgccga tcaacgcggc caggaacacc
     2221 gccaattcgc cggtatcgcg gacgtaattg agctgcagat aggaggcgaa cctctcgttg
     2281 cccaccagat aggtgatgag cacgaaagcg ccggccgcga tcattaccgg catgatcgcc
     2341 agcccgtcga ggccgtcggt gaagttcacc gcattggccg cgccggcgat gacgacgccg
     2401 ccgaccagga tgaaggcgaa gcccaacggc agcagtacgg atttgaagaa cggcaccgcc
     2461 agcatgccgc tttgggaggg agactcgacc tgcatgatca gccaggtcgc caggaaggcc
     2521 acgccgaact cgatcaagag acgcagcttg ccgctgatgc cggcggagga gtgtctggtg
     2581 actttcagat aatcgtcggc aaaaccgagc aggccgtagc tcaccgtcac caggatcacg
     2641 atccagacat acgcattcga gagatcggcc catagcagcg tcgctaccag accgggaaca
     2701 agaatgagca ggccgcccat ggtcggcgtg ccctgttttt caacgacatg gcgcttgggc
     2761 ccgtcgtcgc ggatgggctg ccccatgccc tgtttgagtt tcagccagtt gatcagcggc
     2821 ggtccgatta cgaaagcaac aaacagcgcc gtcagcaccg cgccgcccga acggaaggtg
     2881 aggtatttga acagccgcag gacggcgtaa tggtccgcat gagggagata aaagagaaaa
     2941 tgaaacatgg ttccccctaa cccgcgcgcg ctttcagcgc ttcgacgatg acggacatgc
     3001 ggctgccgtt ggagcctttg acgagtacgg tgtcgcccgc acgcagcgcc gaggcgacgt
     3061 ccggcgcgag cgcggcggag ttaggcgcat aggcggcgcg ctgcggcgac ggcaaaacct
     3121 cccacaaggc ttgcatcagc gggccgcaga gaaaaacgag atcgacgcgc gcggcggcga
     3181 gcgcggcggt gaggccggca tggtgggcgg gcgcgccggg ccccatctcc agcatgtcgc
     3241 ccagcacggc gatgcggcgg ccgggcgcct tgcccagcag cgacagcgcc gccgccatcg
     3301 aagccggatt ggcgttgtag ctttcgtcga tcacctcgac gccgccggcg gcgaagcgcg
     3361 cgccgcggcc ttggagcgcg gaaaatttct tgagcgcggc cgccgcgttc agcacgtcgc
     3421 cctcggcggc ggcaacgcac aaaagcgcgc ccagcgcgtt ggcggcattg tgcgaacccg
     3481 gcgcgccgag gaaaaaatca accggctggc cgagaatgtc ggccttgacg cgggcaccct
     3541 cgccgctctc ctcataggac agaagccgcg cctcgctgcc cggtttggcg ccgaagccga
     3601 gaatccgcgc cacatgggac tgcttggcgc gcgcaatcag gcgttcggca taggggttat
     3661 cggaagggat cagcgcggcg ccgcccgcca cgacgccttc gaaaatctcg gacttggcat
     3721 cgacgatggc ctcgcaggtg ccgaagaact ccagatgcgc cggcgcgatg gtggtgacca
     3781 gcgccacatg cggacgggcg aacgagacca gcggccgtat ttcgccgaaa tggttcatgc
     3841 cgatctcgac gactccgaac gcggcttcgc gcggcaacga ggcgagcgtc agcggcacgc
     3901 cccagtgatt gttcaaggag gcgcgcgagg catgggtctt gcccaaggcg ccgaaagcga
     3961 gcttcagcat ttccttggcc gtggtcttgc cggcgctgcc ggtgaccgcg acgattttcg
     4021 cgtcgatgcg cgcgcgcgag gcaaccgcca gatcctccag cgcgcgctgg gtgttggcga
     4081 ccgcgatctg ggggccgccg gcgccgtcgg gcgtcacgcc cccggacggg cg
//
LOCUS       DASWPU010000191         4081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_69102,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000191 DASWPU010000000
VERSION     DASWPU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69102"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGC27_04250"
     CDS             <1..415
                     /locus_tag="VGC27_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0281820.1"
                     /translation="GVLPGGRVSLKEKIGYSLGDAASNLYWKTFEFRLIIFYTGVFDI
                     SAATVGTMLLVTRKADAGADPVMGAIADQTRTRRGYFRPYLIWFALPPAAAGVLTFTT
                     SNLGADGKAARQHRLAGLPRDGVKWRVTNRTNPPA"
     gene            431..3952
                     /locus_tag="VGC27_04255"
     CDS             431..3952
                     /locus_tag="VGC27_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281821.1"
                     /translation="MTLQNPSRRASLLSRKDRDPSGRDGEPAEAAYYVADAALHDPLH
                     EAEGALIDIAGEPFYCVTHYDAMAPFLMSLVSDSDLWMFVSSTGALTAGRRDADNALF
                     PYYTDDHIHDSQDQTGAKTILRVLCRGRTQLWEPFSERYKGLYRITRRLSKSAYGNKL
                     IFEEVNHDLELSFSSAWLISERFGFVRRAALQNLAIGPVEVVLLDGLQNLMPSGLTRR
                     FQMEYSTLADGYKESELHRASGLGLFRLSAIPADQPKPREALRATTVWSEGIEASRRL
                     LCTAQIDRFRRGLQIAEEALIRGRRGAYFVQTALTLAAQERKEWSLIAEVAQDAAAVA
                     ALLNRLKFPDALRTEIDANIADGTGNLARLIAGADGLQMTGDRLNVWRHFSNALFNSM
                     RGGIPDDDYTVSKADFMAFLSQANRRVHSEQAEALRALPEKLRHDLLVTWARNQQDPD
                     LERLAYEYLPLTFSRRHGDPSRPWNIFSIEVKGEHGEKILNYQGNWRDIFQNWEALAH
                     SYPGFVESMIFKFVDASTADGYNPYRITRNGFDWEVLDAHDAWSYIGYWGDHQVIYLL
                     KLLEISSRYHPGALNGLLTRRVFTYADVPYRIKPYQDTLADPHNTIEFDAALDRRIKQ
                     RAAEMGTDGKSLLGADGTPCRANLTEKLLVLILARFFNYIPAAGLWMNTQRPEWNDAN
                     NALVGNGVSMVTLCYLRRFLSFARDLFATSELEAVEVLVPVEAAFRSVAATLEEYSHL
                     LDGEISDRTRKLVLDRLGAAGSAYRAQLYGSELSDAQTSLAIDRVVAFCDVGLRHIDH
                     SIRANRRADGLYHAYNLMRQEGDGIAISPLQEMLEGQVAVLSSGTLSVRESAELLDAL
                     RASKLYRADQNSYILYPDKILPGFLQKNNIPADALADCKTLTDMIARGDRRIVSEDVN
                     GVAHFNAAFCNASMLEDALLSLGLPADEQRRILALYEKVFNHRAFTGRSGAFYKYEGL
                     GCIYWHMVSKLLLEVQDVIRRAAAMPDSRADLERLQRQYREIRDGIGSHKSPAAYGAI
                     PLDPYSHTPGFAGVQQPGMTGQVKEDIISRFGELGAVVQAGRLGFSAPMVNHSEFLRE
                     PVRFQYVGIGGRDSHIDLDPGTAAFTLCQVPVVLHAGGIAGIEVSYADGVRKTVDGLV
                     LDVAVSAAIFERRDVVRRLDVFLGRRA"
BASE COUNT          777 a         1222 c         1250 g          832 t
ORIGIN      
        1 gggggtgttg ccgggcgggc gcgtttctct gaaagagaag atcggctatt ccctgggcga
       61 tgcggcgtcg aacctctatt ggaagacgtt cgaattccgt ttgataatat tctacacggg
      121 tgtcttcgac atctcggccg ccacggtcgg cacgatgctg ctcgtcaccc gcaaggcgga
      181 cgccggtgcc gatccggtga tgggcgcgat cgccgatcaa acacgcacgc gtcggggcta
      241 ttttcgccct tatctgatct ggttcgcctt gcctccggct gcggccggcg tgctgacctt
      301 taccacgtcg aatttgggcg ctgatggcaa agccgcgcga caacaccggt tggcgggttt
      361 gccgcgggac ggcgtcaagt ggcgcgttac gaacagaacc aatccaccgg catgaatcgc
      421 ggccacagct atgaccctgc aaaatccctc acggcgcgcg tctcttcttt cccggaaaga
      481 tcgcgaccca agcggccgag acggtgaacc ggcggaagcg gcatattatg tcgctgatgc
      541 cgcgctgcac gatccgctcc acgaagccga aggtgcgctg atcgatatcg cgggcgagcc
      601 tttctattgc gtcacgcatt acgatgcgat ggcacccttc ctgatgagcc ttgtcagcga
      661 ttccgattta tggatgttcg tctccagcac cggcgccctc actgccggcc ggcgcgacgc
      721 cgataatgcg ttgtttccct actataccga tgatcacatc catgacagtc aggatcaaac
      781 cggggccaag accatattgc gggtcttgtg ccggggccgc acgcagctct gggaaccatt
      841 tagcgagcgc tataagggtc tttaccggat tacgcgccgg ctgtcgaaaa gcgcctatgg
      901 caacaagctg attttcgagg aggtcaatca cgatctcgaa ctatcgttct caagcgcatg
      961 gctgatcagc gagcgctttg gttttgtccg ccgcgccgcg ttgcagaacc ttgccatcgg
     1021 gccggtcgag gtcgtgttgc tggatggcct gcaaaacctg atgccgagcg ggttgacccg
     1081 ccgctttcag atggaatatt cgacgctcgc cgacggctac aaggaaagcg aactgcaccg
     1141 cgccagtggt cttgggttgt tccggctgag tgcgataccg gccgatcagc ccaagccacg
     1201 cgaggcgctc cgcgcaacca ccgtctggtc cgagggcatc gaggcatcac gccggctgct
     1261 ctgcacggcg cagatcgatc ggttccgccg tggactgcag atcgcggagg aagcgctgat
     1321 ccgcggccgc cgcggcgctt atttcgtgca aaccgctttg acgcttgcgg cacaagagcg
     1381 aaaggagtgg agcctcatcg ccgaggtggc gcaggacgcg gcagcggttg ccgcccttct
     1441 caaccggctg aaattcccgg atgccctgcg caccgagatc gatgcgaata tcgccgacgg
     1501 caccgggaat ctggcgcgcc tcatcgccgg tgccgacggt ctgcaaatga ccggcgaccg
     1561 gttgaatgtc tggcggcatt tttccaatgc gctgttcaat tccatgcgcg gcggcatccc
     1621 ggacgatgac tacaccgtct cgaaggccga ttttatggcg ttcctctccc aggccaatcg
     1681 ccgggtgcat agcgaacagg cggaagccct tcgggcgctg ccagaaaaat tgcgccacga
     1741 cctgctggtg acatgggcac gcaatcaaca ggatcccgac ctggagcgcc tggcctacga
     1801 atatttgccg ctgaccttca gccgccgcca cggcgacccc agccggccat ggaacatctt
     1861 ctccatcgag gtgaaaggcg agcatggcga aaagattctg aactatcagg gcaattggcg
     1921 cgacattttc cagaactggg aggcgctggc gcattcctat cccggttttg tcgaaagcat
     1981 gatcttcaaa ttcgtcgatg catccaccgc cgacggctac aacccgtatc gtatcacgcg
     2041 caatgggttc gattgggaag tcctcgatgc gcatgatgcc tggtcctata tcggttattg
     2101 gggggaccat caggtcatct acctgctcaa actgcttgaa atctcgtcgc gctatcatcc
     2161 cggtgcgctg aatggtctgc tcacaagacg ggtgttcacc tatgccgacg tgccttatcg
     2221 gataaagccc taccaggaca cgttggcgga tccgcacaac accatagagt ttgatgccgc
     2281 gcttgatcgc cggatcaagc agcgtgccgc ggagatgggc acggacggca agtcgctatt
     2341 gggcgcagac ggcacgcctt gtcgcgcgaa cctgacggaa aagcttctgg tgctgatttt
     2401 ggcgcggttc ttcaattaca tccccgccgc cggattgtgg atgaatacgc agcggccgga
     2461 atggaacgac gccaataatg cgttggtcgg caatggcgtg tcgatggtca cgctatgcta
     2521 tctgcgccgt ttcctgtcgt ttgcgcgcga tctctttgcc acatccgagc tggaagccgt
     2581 cgaggtgctg gtgccggtcg aagccgcctt ccggagcgta gccgccacac tggaggaata
     2641 tagccatctg ctggatggag agatttcgga ccgcacgcgc aagctggtgc tcgaccggct
     2701 gggcgccgcc ggcagcgcct accgggcgca actttatggc agcgaattat ccgatgcgca
     2761 aacatcgctg gcgatagatc gtgtcgtggc gttctgtgat gtcggcctac ggcacatcga
     2821 tcactctatc cgtgccaatc ggcgcgccga cgggctttat cacgcctaca atttgatgcg
     2881 gcaggagggt gacggcatcg cgatcagccc cttgcaggag atgctggaag ggcaggtggc
     2941 ggtactgagt tccggcacct tgtcggtccg cgagtcggcc gagctgctgg atgccttgcg
     3001 cgccagcaag ctctaccgcg ccgatcaaaa cagctacatt ctctatcccg acaagatttt
     3061 gccgggcttc ctgcagaaga acaacatccc ggctgacgcc ctggcggact gcaaaaccct
     3121 gaccgacatg atcgcgcgcg gcgataggcg catcgtgagc gaggacgtca atggcgtcgc
     3181 tcacttcaat gccgcgtttt gcaatgcctc gatgctggag gacgcacttc tctcgctagg
     3241 cctgccggcc gacgagcaga ggcgcatact cgctctgtat gaaaaggtgt tcaaccaccg
     3301 cgccttcacc ggccgctccg gcgccttcta caaatatgaa gggcttggct gcatctattg
     3361 gcatatggtg tccaagctat tgctggaagt gcaggacgtg atacggcgcg cagcggccat
     3421 gccggacagc cgggcagacc tcgagcggtt gcaaagacag tatcgcgaga tccgcgacgg
     3481 catcggcagc cacaaatcgc cggcggccta tggcgcgatt ccgctcgatc cttattcgca
     3541 tacgccgggt tttgccgggg ttcagcagcc agggatgacc gggcaggtta aggaggatat
     3601 catctcgcgc tttggcgaat tgggcgccgt cgtccaggcc ggccggcttg gtttctctgc
     3661 gccgatggtc aaccacagtg aatttctgcg cgagcctgtc cgctttcaat atgtcggtat
     3721 cggcggacgg gacagtcata tcgatcttga tcccggaacg gcggccttca ccctgtgtca
     3781 ggtgccggtc gtgctgcatg ccggcggcat tgccgggatc gaggtctcct acgccgatgg
     3841 cgtgcgtaaa acggtggacg gccttgttct cgacgtcgcg gtcagcgccg ccattttcga
     3901 gcggcgggat gtggttcggc ggctggatgt cttcctcggg cggcgcgctt agagcaaaat
     3961 acgatcatac agaatcaagc gtgtcatgcc cggcgagcgc gcagcgcgag ggaagggtat
     4021 ccagaaatct gtgcaggcga atggattccc ttcccctcgc tacgctcggc cgggaatgac
     4081 a
//
LOCUS       DASWPU010000192         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_62878,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000192 DASWPU010000000
VERSION     DASWPU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62878"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..485
                     /gene="rpsF"
                     /locus_tag="VGC27_04260"
     CDS             138..485
                     /gene="rpsF"
                     /locus_tag="VGC27_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY0281822.1"
                     /translation="MSSQAVDALATHLKTSVEGEGGKVEKQEYWGLRGLAYRIKKNRK
                     GHYVLLNINAPSKAVIELERQLKINEDVLRFLTVKVDAFEVSSNKARRDDAAGLVAKP
                     EGAVVAETEEIAK"
     gene            578..799
                     /gene="rpsR"
                     /locus_tag="VGC27_04265"
                     /pseudo
     CDS             578..799
                     /gene="rpsR"
                     /locus_tag="VGC27_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            809..1417
                     /gene="rplI"
                     /locus_tag="VGC27_04270"
     CDS             809..1417
                     /gene="rplI"
                     /locus_tag="VGC27_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY0281823.1"
                     /translation="MQVILLERVEKLGQMGDEVKVKDGFARNFLLPQKKALRATKANR
                     EYFTVQKAQLEAQNLTRKKDAEAVSVKLAGKVFVLLRQAGDRGQLYGSVSPRDIADVI
                     TAGGFTVSRTQVPLDKPIKHIGLVPVNVVLHPEVRVPITLNVARSEEEAERQERGEDV
                     LAEKTEAEEAVVAAEELFEEGAGPKEETEGEEPAEDEKKAEE"
BASE COUNT          457 a          644 c          659 g          404 t
ORIGIN      
        1 ctcaggcggg tatgcgcatc gtgcgcgttt caagtaatcc agtgcgagtt caagggctta
       61 gccctgccac tcacatcgta gcctggaaag ggacggatag gtctctatgc gagcatctcc
      121 tgatcgcgcg ctaggatatc agctcgcagg cggtcgatgc gctcgccact cacctcaaaa
      181 ccagcgtcga aggcgaaggc ggcaaggtcg agaaacagga atattggggc cttcgcggtc
      241 ttgcttaccg catcaagaag aaccgcaaag ggcattacgt gctcctcaac atcaacgcgc
      301 cgtccaaggc cgtgatcgag ttggagcgcc agctcaagat caacgaagac gtgctgcgct
      361 tcctgacggt gaaggtggat gcgttcgagg tgtcctcgaa caaggcccgc cgcgacgatg
      421 ccgccggcct tgtcgccaaa cccgaaggcg ccgttgtcgc cgaaaccgag gagatcgcga
      481 aatgagttac gcaggcggac attctggcgg ccgcggcggc gatcgcggag accgtggaga
      541 tcgcggcgaa ggcggacatg gcggagcggg cggtcagcgc cggcccttct tccgccgtaa
      601 gaagacctgt cccttctcgg gcgcgggcgc accgaagatc gattacaagg acgtgaagct
      661 gctgcaacgc ttcatctcgg agcgcggcaa gatcgtgccg tcgcgcatca ccgcggtttc
      721 gaccaagaag cagcgcctgc tcgccaacgc gatcaagcgc gcccgcttca tggcgcttct
      781 gccctacgtc gtggaatagg agagcgccat gcaagtgatt ttgctcgaac gcgtcgaaaa
      841 gctcggccag atgggcgacg aagtgaaggt gaaggacggc ttcgcccgca acttcctctt
      901 gccccagaag aaggccctgc gcgccaccaa ggccaatcgc gaatatttca ccgtccagaa
      961 agcgcagctc gaagcccaga acctgacgcg caagaaggac gccgaggcgg tgtcggtgaa
     1021 actcgccggc aaggtcttcg tcctgctgcg ccaggcgggc gaccgcggcc agctctacgg
     1081 ctcggttagc ccacgcgaca tcgccgatgt catcaccgcc ggcggcttca ccgtctcgcg
     1141 cacgcaagtg ccgctggaca agccgatcaa gcatatcggg ctggtgccgg tgaacgtcgt
     1201 gctgcatccg gaagtgcgcg tgccgatcac gctcaatgtc gcgcgcagcg aggaagaggc
     1261 cgaacgccag gaacgcggcg aagacgtgct cgcggagaag acggaggccg aagaagccgt
     1321 cgtcgccgcc gaggaactgt tcgaagaagg cgccggcccg aaggaagaaa ccgaaggcga
     1381 agagcctgcc gaagacgaga aaaaagctga agagtaaggt ttttaattcc ttcacatgga
     1441 ctggaaaagg cccggcttcc gccgggcctt tttcgttggt gtatcagttt ggggtgccct
     1501 aacttctagg ctttagagcc cacagaccac gaggtagatc aggatcaatc ttgcccgcat
     1561 ttaccagctt gcgtcgtgct tgacgaactc ggttctccca atgcacttca tcgctgttcg
     1621 gcatcattct atgccgctgc cccggtgtca gcccaaaccg atcagcgagt gctctatatg
     1681 catccgaagg gcctattggg cgttggagcc ctgccaagta gtcttcgagt tcagcaatga
     1741 ccgagttttc ggttggtagg tctgctacgt ttgtttggcc taacggcatg gaatctgcct
     1801 cctctccatt caggttatca gagctagatt gtgttccgcg ccttggtcga aggcttgcaa
     1861 gcggcaccca agctgcatca cttggccgcc tgacgttcca ataaacccaa ccgtctaggc
     1921 tggttcgagc accattcttt gtaagggcaa cgcctccagc ggcggcggag gggctgttgt
     1981 atcgcttgcc ttctaccgtc cagtcaccgt tatcgatgac gccagtatga acacgtccgt
     2041 tgtagtccat ctggatttct gtaccgtgag gcaggactac gcctttgcct gcccaagggc
     2101 gcccgacaac cagggacgaa acgggttgct cgctgtcgat gcgcagaagg cggcgcaaca
     2161 ccga
//
LOCUS       DASWPU010000193         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_1448, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000193 DASWPU010000000
VERSION     DASWPU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..605)
                     /gene="coaD"
                     /locus_tag="VGC27_04275"
     CDS             complement(84..605)
                     /gene="coaD"
                     /locus_tag="VGC27_04275"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY0281824.1"
                     /translation="MTKKRIALYPGTFDPLTLGHLDIVERAVKLVDHLVIGVAANPAK
                     SPTFTQNERVAMVRHETRPLAGNGRAAISVQGFDRLLIEFAEEVGAGMIVRGLRAVSD
                     FEYEFQMVGMNQRLNPEIETVFLMADPRHQAIASRFVKEIAMLGGDVSGFTSPHVAEL
                     LAKRYRKLGNKKK"
     gene            complement(605..>3387)
                     /gene="gyrA"
                     /locus_tag="VGC27_04280"
     CDS             complement(605..>3387)
                     /gene="gyrA"
                     /locus_tag="VGC27_04280"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY0281825.1"
                     /translation="VNWRQTVAHFAGLRSKKDQDLSEVPPSAPPPSGASVNPIAIEDE
                     LKRSYLDYAMSVIVARALPDARDGLKPVHRRILHSMNENGYDWNKAYRKSARVVGDVI
                     GKYHPHGDQSIYDALVRMAQDFSMRAPLIDGQGNFGSIDGDPPAAMRYTEVRRAKIAH
                     ALTEDIDKDTVDFQDNYDGSEKEPVVLPARFPNLLVNGASGIAVGMATNVPPHNLGEV
                     IDACLAYIDDPSIGLDALTEIVPGPDFPTGALIIGKQAARGALARGRGSILMRARCHI
                     EEIRKDREAVIVTEIPYQVNKARMQERIAELVRDKRIEGISDIRDESDRHGMRVVIEL
                     KRDASSEVVLNQLYRFSELQTSFGVNMLALDDGQPRLMNLKQLLAVFVLFREEVITKR
                     TRFELGKARDRAHILAGLAVAVANIDEVIALIRAAPDANTARERLMAQAWPAKDVGPL
                     VALIADPRNSIAEDGTIKLTEEQAKAILDIRLQRLTALGRDEIGEEVKKLSEAIKDYL
                     EILRSRPRVLGIVKSELEAVKAEFATPRLTELVDAETEIEDEALIEREEVAITVTHAG
                     YIKRTPIAEYRVQGRGGKGRSGMVTREEDFVTNLFVASTHAPLVFFSSTGMAYKLKVW
                     RLPEARIAGKGKAMVNLLPLSEGERITTILPLPEDETQWEKFNVVFATKSGNVRRNEL
                     SDFVSINKSGKIAMKLAPGDGIVGVQICTDRDDVLLTTRGGKCIRFPLTDVRVFKGRE
                     STGVRGIKLAAGDEVVSLTLLHHSDATTAEARTYLKQSSAARRAATGEEEAPVEETEE
                     TEENGEEATLTPERYAGLGAKDQFVLAVSENGFGKRTSSYEYRVTGRGGSGIVAMGMG
                     RKNSAIIAAFPVEESDDLMLISNTGQTIRVAVGSISVQGRAAQGVTVFRVEDAERVVS
                     VERIEDVSEEGG"
BASE COUNT          512 a         1080 c         1127 g          668 t
ORIGIN      
        1 ggcgggcgcc agatcgggct tcggctcgat gaccaccggg ccggtcttca actccatgat
       61 caaagtgttg ttcggatcgc ggctcatttc ttcttgttcc ccaatttccg gtagcgcttc
      121 gccaacaact cggcgacatg agggctggtg aacccgctca cgtcgccccc cagcatggcg
      181 atttccttga cgaagcggga ggcgatggcc tggtggcgcg gatcggccat cagaaacacg
      241 gtctcgatct cgggattgag ccgctggttc atgcccacca tctggaattc gtattcgaag
      301 tcggagacgg cgcgcagtcc gcgcacgatc attcccgcgc cgacctcttc ggcgaattcg
      361 atcagcagcc ggtcgaagcc ctgcaccgag atggcggcgc gcccgttgcc ggcgagcggc
      421 ctggtctcgt ggcggaccat cgcgacgcgt tcgttctgcg tgaacgtcgg cgatttggcc
      481 ggattggcgg cgacgccgat gaccaggtga tcgaccagct tgaccgcccg ttcgacgatg
      541 tcgagatgac ccagcgtcag gggatcgaag gtgcccggat aaagggcaat gcgctttttc
      601 gtcatcagcc gccttcttcc gaaacgtctt cgatgcgttc gaccgaaacc acgcgctcgg
      661 cgtcttccac ccggaagacc gtcacgccct gagccgcgcg gccctgtacg ctgatgctgc
      721 cgaccgccac gcggatggtc tgtccggtat tgctgatcag catcagatcg tcggattcct
      781 cgaccgggaa ggcggcgatg atggcgctgt tcttcctgcc catgcccatg gcgacgatgc
      841 cggagccgcc gcgcccggtg acgcggtatt cgtaggaact ggtgcgcttg ccgaagccgt
      901 tttccgacac cgcgagcacg aactggtcct tggcgccgag cccggcatag cgttccggcg
      961 tcagcgtcgc ctcttccccg ttctcttcgg tctcctcggt ctcttcgacg ggcgcttcct
     1021 cttcgccggt ggcggcgcgg cgcgccgcgc tcgactgctt cagataggtg cgcgcctcgg
     1081 cggtggtggc gtcggagtgg tgcagcaagg tcaggctcac cacttcgtcg cccgcggcca
     1141 gcttgatgcc gcgcacgccg gtggattcgc ggcccttgaa cacgcgcacg tcggtcaagg
     1201 ggaaacggat gcacttgccg ccccgcgtgg tgagcagcac gtcgtcgcgg tcggtgcaga
     1261 tctgcacccc gacgatgccg tcgccgggcg ccagcttcat ggcgatcttg ccgctcttgt
     1321 tgatgctgac gaagtcggac agttcgttgc gccggacgtt gcccgacttg gtggcgaaga
     1381 cgacgttgaa cttctcccac tgggtctcgt cttcgggcag gggcagaatg gtggtgatgc
     1441 gttcgccctc ggagagcggc aacaggttca ccatcgcctt gcctttgccg gcgatgcgcg
     1501 cctcgggcag ccgccagacc ttcagcttgt aggccatgcc ggtggaagaa aagaacacca
     1561 gcggcgcgtg ggtcgaggcc acgaacaggt tggtgacgaa atcctcttcg cgggtgacca
     1621 tgcccgagcg gcccttgccg ccgcggccct ggacgcgata ttcggcgatg ggcgtgcgct
     1681 tgatatagcc cgcatgggtg acggtgatcg ccacctcctc gcgctcgatc agcgcttcgt
     1741 cctcgatctc ggtttcggcg tcgaccagtt cggtcaggcg cggggtggcg aactcggcct
     1801 tcaccgcttc cagttcgctc ttgacgatgc ccagcacgcg cgggcgcgag cgcaggattt
     1861 cgagataatc cttgatcgcc tcgctgagtt tcttgacctc ttcgccgatt tcgtcgcggc
     1921 cgagcgcggt gaggcgctgc aagcggatgt cgaggatggc cttggcctgt tcttcggtca
     1981 gcttgatggt gccgtcttcg gcgatggaat tgcgcgggtc ggcgatcagc gccaccagcg
     2041 gccccacatc tttcgccggc caggcttgcg ccatcagccg ctcgcgagcg gtgttggcgt
     2101 ccggcgcggc gcggatcagg gcgatcacct cgtcgatgtt ggccacggcc acggcgagac
     2161 cggccaggat atgcgcccgg tcgcgcgcct tgcccagttc gaaacgggtg cgcttggtga
     2221 tgacctcttc gcggaacaga acgaagaccg ccagcagttg cttcaggttc atcagccgcg
     2281 gctggccgtc gtcgagcgcc agcatgttga cgccgaagct ggtctgcaat tccgagaagc
     2341 gatagagctg gttgagcacg acttccgacg aggcgtcgcg cttcagctcg atgaccacgc
     2401 gcatgccgtg gcggtcgctt tcgtcgcgga tgtcggagat gccttcgatg cgcttgtcgc
     2461 gcaccagttc ggcgatgcgc tcctgcatcc gcgccttgtt cacctgatag gggatctcgg
     2521 tgacgatgac ggcttcgcgg tccttgcgga tttcctcgat gtggcaacgc gcccgcatca
     2581 ggatcgagcc gcgcccgcgc gccagcgcgc cgcgcgccgc ctgcttgccg atgatcagcg
     2641 ccccggtcgg aaagtccggt cccggcacga tctcggtcag cgcgtccagg ccgatggacg
     2701 gatcgtcgat ataggccagg caggcgtcga tcacctcgcc cagattgtgg ggcggcacgt
     2761 tggtcgccat gcccaccgcg atgccgctgg cgccgttgac caagagattg ggaaagcggg
     2821 cgggaagaac gacgggttcc ttttccgagc cgtcgtaatt gtcctggaaa tcgacggtgt
     2881 ccttgtcgat gtcctcggtc agcgcatggg cgatcttggc gcggcgcact tcggtgtagc
     2941 gcatggcggc cggcggatcg ccgtcgatcg aaccgaaatt gccctgcccg tcgatcagcg
     3001 gggcgcgcat cgagaagtcc tgcgccatgc gcaccagcgc gtcgtagatc gactggtcgc
     3061 cgtgcggatg gtatttgccg atgacgtcgc cgaccacgcg ggcggacttg cggtacgcct
     3121 tgttccagtc gtagccgttc tcgttcatcg aatggaggat gcgccggtgc accggcttca
     3181 gtccgtcgcg ggcgtcgggc agcgcgcgcg cgacgatcac gctcatcgcg tagtcgagat
     3241 aggagcgctt gagctcgtcc tcgatggcga tgggattgac gctggcgccc gaaggcggag
     3301 gggcggacgg gggaacttcg ctcaaatcct ggtccttctt ggagcgcaat ccggcaaagt
     3361 gtgcaacggt ttgccgccag tttacgc
//
LOCUS       DASWPU010000194         3986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_44731,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000194 DASWPU010000000
VERSION     DASWPU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44731"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VGC27_04285"
     CDS             complement(<1..657)
                     /locus_tag="VGC27_04285"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0281826.1"
                     /translation="MTTIDAWVARVRNTKAYDIFVALPLIALFGAGAWKEAHTILAHM
                     RLIADGTEDLLGVLQMLALIGSIIFILLLIVMLLVRTLPRARAPGILPRVLAVTGTLL
                     GTGFLYLQPVVLPVWFQAVVNVMIFGASVLEIVILLRLGKSFAIMAEARELVTTGPYA
                     VVRHPLYVAEELGILTMVIQFAGPIAVALMLAHIAVQVARTVYEERVLTATFPEYSAY
                     K"
     gene            1020..3914
                     /gene="uvrA"
                     /locus_tag="VGC27_04290"
     CDS             1020..3914
                     /gene="uvrA"
                     /locus_tag="VGC27_04290"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004813679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0281827.1"
                     /translation="MLKNISIRGARQHNLKNVDVDIPRDKLTVMTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLELMQKPDVDHIEGLSPAIAIEQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGVPYSPATGLPIESQTVSQMADRILALPAGTRLYLNAPIVRGRK
                     GEYRKDLLDLQKKGFQRVKVDGKYYDIASTPALDKKLKHDIEVVVDRIAVKPDIGNRL
                     PDSIETALKLSDGLVIAEFADEKDKDGKPRQLLMSSKFACPVSGFTISEIEPRLFSFN
                     SPHGACPACDGLGTQLYFDPALIVPDAALTLRKGALSPWHKSNSPFYLQTLEALARHY
                     KFSLNDPWEDLSKKVKSIILYGSGEDEIKFIYDDGMRRYETKKTFEGVIPNMQRRYKE
                     TDSAWIREEMERFQDASPCEVCSGYRLKPEALAVKIAGLHISQVCEKSIRLADAWFRD
                     LDRHLTKQQQEIGARILKEIRARLKFLNNVGLEYLTLARASGSLSGGESQRIRLASQI
                     GSGLTGVLYVLDEPSIGLHHRDNAKLLESLKGLRDLGNTVIVVEHDEEAIRTADHVID
                     MGPGAGIHGGHIVAEGTPEDIMRNPKSLTGKYLVGLEQIPIPAKRRGALHNRWLKIVG
                     ARGNNLKNVTADVPLGTLTCVTGVSGGGKSTLVIETLFKAASRKLHQSREHPMPFDKI
                     EGLEFLDKVIDIDQSPIGRTPRSNPATYTGAFTPIRDWFTGLPEAKTRGYAPGRFSFN
                     VKGGRCEACQGDGVIEIEMHFLPDVYVTCDVCGGKRYNRETLEVKFRDHSIADVLDMT
                     VEAGAELFKAVPSIREKLDTLKRVGLGYISVGQQATTLSGGEAQRVKLSKELSRRATG
                     RTLYILDEPTTGLHFHDVRKLLEVLHELVDNGNTVVVIEHNLEVVKTADWIVDLGPEG
                     GDNGGEIVAQGTPEEVAKVARSYTGQYLKTLLKGRTPEKASSARKMKAAE"
BASE COUNT          827 a         1294 c         1193 g          672 t
ORIGIN      
        1 cttataggcg ctgtattccg gaaaggtcgc ggtgagaacc cgctcttcgt agaccgtcct
       61 tgcgacctgt acggcgatat gcgcaagcat gagtgcgacg gcgatgggcc ccgcgaactg
      121 aatcaccatc gtcaggatgc cgagttcttc cgccacatag agcggatggc gcaccacggc
      181 atagggaccg gtcgtcacca gctcgcgcgc ttcggccatg atggcgaagg attttcccag
      241 ccgcagcaga atgacgatct caagtacgct ggcgccgaaa atcatgacgt tcacgaccgc
      301 ctggaaccag accggcagga cgaccggctg caggtagaga aagcccgttc ccagaagagt
      361 ccccgtcacg gcgagcacac gcggcaggat gcccggcgcc cgcgcgcgcg gcaatgtccg
      421 taccaacagc atgacgatga gcaacaagat gaaaatgatg gaaccgatga gggccagcat
      481 ttgcagcacc cccagcagat cttcggtgcc gtctgcaatc agccgcatgt gcgcgaggat
      541 cgtgtgcgcc tctttccatg cccccgcccc gaacaaggcg atcagcggca gagccacgaa
      601 aatgtcgtaa gccttggtgt ttcggacccg cgccacccac gcgtcgatcg tcgtcatcgg
      661 catgcccccc accccttcat ctgcctatca caagaggccg gagacaggcg agccatctcc
      721 gctgctcctt ccggccggct cttcggcggt tctggacagc atttcagact tgtgcaatta
      781 attgttcttg atttgttcat gagtggcgcg acgatgttcg atctcggggc ttttctcggc
      841 gggaagacga tgaaggataa gccttgcggt tccttgtgga catcgccaca tcagagcttc
      901 ccgtcgggaa ccggccgatt tggacgaatt cggcggtccg tggaacgtca aagaaatctc
      961 gcatggttgt ggtgggaaag ctcccacacc acagtgtgcg tttgtccaag gtcgaaagaa
     1021 tgctcaaaaa catctccatc cgcggtgccc gccagcacaa tctgaagaac gtcgacgtcg
     1081 atattccccg cgacaagctc accgtgatga ccgggctgtc cggctccggc aaatcctcgc
     1141 tcgccttcga tacgatctat gccgagggcc agcgccgcta cgtggaatcg ctgtcggctt
     1201 atgcgcgcca gttcctggag ctgatgcaga agcccgacgt ggaccatatc gagggcttgt
     1261 cgcccgccat cgccatcgag cagaaaacca cctccaagaa tccgcgctcc accgtcggca
     1321 cggtgacgga gatctacgat tacctgcgcc tgctgttcgc gcgcgtcggc gtgccctatt
     1381 cgccggccac cggcctgccg atcgaaagcc agaccgtcag ccagatggcc gaccgcatcc
     1441 tggcgctgcc cgccggcacg cggctctacc tcaacgcccc catcgtgcgc ggccgcaaag
     1501 gcgagtaccg caaggacctc ctcgacctgc agaaaaaggg cttccagcgc gtcaaggtgg
     1561 acggcaaata ttacgacatc gcctcgacgc ccgcgctcga caagaagctc aagcacgaca
     1621 tcgaggtggt ggtggaccgc attgcggtaa agccagacat cggcaaccgc ctgccagatt
     1681 ccatcgagac cgcgctcaag ctctccgacg gcctcgtcat cgccgaattc gccgacgaaa
     1741 aagacaagga cggcaagccg cgccagttgc tgatgtcgtc gaaattcgcc tgcccggtgt
     1801 ccggcttcac catttcggag atcgagccgc ggctgttctc gttcaacagc ccgcacggcg
     1861 cctgcccggc ctgcgacggg ctgggcaccc agctttattt cgatccggcg ctgatcgtgc
     1921 ccgatgctgc gctgacgctg cgcaagggcg cgctgtcgcc ctggcacaaa tccaattcgc
     1981 ccttctatct gcaaaccctg gaggcgctgg cgcggcacta caaattctcg ctcaacgatc
     2041 cgtgggaaga cctgtccaaa aaggtgaaga gcatcatcct ctacggctcc ggcgaggatg
     2101 agatcaaatt catctacgac gacggcatgc gccgctacga gacgaagaaa accttcgagg
     2161 gcgtgattcc caacatgcag cgccgttaca aggaaacgga ctccgcctgg atccgcgagg
     2221 agatggagcg cttccaggac gccagcccgt gcgaggtctg tagcggctac cgcctcaagc
     2281 ccgaagcgct ggcggtgaag atcgcggggc tgcacatcag ccaggtctgc gagaagtcca
     2341 tccggctggc cgacgcctgg ttccgcgatc tcgaccggca cttgaccaag cagcagcagg
     2401 aaatcggcgc gcgcatcctc aaggaaattc gcgcgcggct taagttcctc aacaatgtcg
     2461 gcctcgaata tctcaccctg gcgcgcgctt ccggctcgct gtcgggcggc gaaagccagc
     2521 gcatccggct ggcctcgcag atcggctccg gcctgaccgg cgtgctttac gtgctcgacg
     2581 agccgtccat cggcctgcat caccgcgaca acgccaagct cttggaatcc ctcaagggcc
     2641 tgcgcgatct cggcaacacc gtgatcgtgg tggagcatga cgaggaggcg atccgcaccg
     2701 ccgatcacgt gatcgacatg gggccgggcg ccggcatcca tggcgggcac atcgtcgccg
     2761 aaggcacgcc cgaagacatt atgcgcaatc ccaaaagcct gacggggaaa tatctcgtcg
     2821 gcctggagca gattccgatt ccggccaagc gccgcggcgc cttgcacaac cgctggctca
     2881 agatcgtcgg cgcgcgcggc aacaatctga agaacgtcac cgcggacgtt ccgctgggca
     2941 cgctgacctg cgtcaccggc gtttcgggcg gcggcaaatc caccctggtg atcgagacgc
     3001 tgttcaaggc ggcgtcgcgc aagctgcacc agagccgcga gcatcccatg ccgttcgaca
     3061 agatcgaggg gctcgaattc ctcgacaagg tgatcgacat cgaccagtcg ccgatcgggc
     3121 gcacaccgcg ctccaatccg gccacctata ccggcgcctt caccccgatc cgcgactggt
     3181 tcaccggact gccggaagcc aagacgcgcg gctacgcgcc ggggcgcttc tcgttcaacg
     3241 tcaagggcgg acgctgcgaa gcctgccagg gcgacggcgt catcgagatc gagatgcact
     3301 ttttgcccga cgtctatgtg acctgcgacg tttgcggcgg caagcgctac aaccgcgaaa
     3361 ccctggaagt gaagttccgc gaccattcca tcgccgacgt gctcgacatg acggtggaag
     3421 ccggcgccga actgttcaag gccgtccctt cgatccgcga gaagctggat acgttgaagc
     3481 gcgtcggcct cggctatatc agcgtcggcc aacaggcgac gacgctgtcg ggcggcgagg
     3541 cgcagcgcgt caagctgtcg aaggagctgt cgcgccgggc caccgggcgc acgctctaca
     3601 tcctcgacga gccgaccacg ggcctgcact tccacgacgt gcgcaagctg ctggaggtgc
     3661 tgcacgaact ggtcgataac ggcaacacgg tggtggtgat cgagcacaat ctggaagtgg
     3721 tgaagacggc cgactggatc gtggacctcg gccctgaagg cggcgacaat ggcggcgaga
     3781 tcgtcgccca aggcacgccg gaagaggtgg cgaaggtggc gcgctcctac accggccaat
     3841 acctcaaaac tttgttgaaa ggccgcacgc ccgaaaaggc gtcatccgca cgtaagatga
     3901 aggcagcgga gtagatctca ccaactaaat atcgaaaccg aagtgacgct tggaactacg
     3961 cagtccctcg tggctcagaa ttggcg
//
LOCUS       DASWPU010000195         5086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_74987,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000195 DASWPU010000000
VERSION     DASWPU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74987"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VGC27_04295"
     CDS             complement(<1..670)
                     /locus_tag="VGC27_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281828.1"
                     /translation="MHVLVLHSDVPPDAPADEQDTLIAAEAVEDALRSRGHEAAQAPF
                     RPDAKALGKMLGRERPDVVFNLVESVFGAGIYASLAPAMLARLGVRFTGGTAAHFAAT
                     TDKVFAKTILRAAALPTPDWASPPAWAGLDEARAYIVKAADEDSSVGLDDACVVKGAE
                     VRARAADSTKRFGGRWFAEAYVEGREFNIGLIQEKDGPRILPMAEMTFEDWPSGRPRI
                     VGYSA"
     gene            complement(670..1791)
                     /locus_tag="VGC27_04300"
     CDS             complement(670..1791)
                     /locus_tag="VGC27_04300"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281829.1"
                     /translation="MRIGVTYDLRADYLALGFSLEETAEFDSEITIAAICEALEGLGH
                     EPVRIGGVRKLAERLVAGERWDAVFNFCEGLKGVAREAQAPALLDAYDIPYVFSDPLT
                     LALALDKSMAKRVVRDAGVPTAPFAVIERLEDAAKVALPFPLFLKPAAEGSGKGVGAR
                     SKVADAASLRAVAADLLARFAQPVLVETFLSGREFTVGITGTGEEAEILGVSEIVPKA
                     KFVGAGYGFENKEAGWEEKVEIVCARGPEAEAAGKVALGAWRALRCRDGGRIDIRCDD
                     KGAPHFIEVNPLAGLRPKHSDLCQIATDAGLSYQDLIGLIMASFLKRHPELAQPLSPP
                     VHGGSVAEGGKGGIHRARPLRSVSRTTSPVNGGGKERVS"
     gene            complement(1918..3210)
                     /locus_tag="VGC27_04305"
     CDS             complement(1918..3210)
                     /locus_tag="VGC27_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEY0281830.1"
                     /translation="MSLQAMRGRELKPPLRPVRPRPALASSLQETNKVTSLAAARAAL
                     RFPVTPETRAFYRRFYPNTSSLEWNDWRWQMRSRIRTLDELARIFALSADERQAVERH
                     EGALPVGITPYYASLMGLSDPSEPLRRTHIMTGGEYVRGPGEADDPLGEDHDTVVPGL
                     VHRYPDRVLFLTTGTCSTYCRYCTRARVVGNPGGEYQFSTRQWDKALAYLEVHTEVRD
                     VLLSGGDPLTIGDDKLDWLLTRLRAIKHIEFLRIGTKIPVVLPQRITKDFVKMLKKHH
                     PLWMSIHFTHPVELTEEVTESTARLADAGIPLGSQTVLLKGINDSPAVMVPLMQGLLK
                     RRVKPYYLYQCDPIRGSAHFRTTVEKGLEIIASLRGHTTGYATPMFCIDAPGGGGKIQ
                     LAPDPVVGRDGDDLLLRNFEGGVYRYPDPGGTLGRGKE"
     gene            complement(3660..>5086)
                     /locus_tag="VGC27_04310"
     CDS             complement(3660..>5086)
                     /locus_tag="VGC27_04310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0281831.1"
                     /translation="RDQIWTYRLPSLIGAVAAVWLAFWCAGAFAGAEVALLAAALLGG
                     TLLLGGEATIATTDAVLLACVLGAQGFLLRVYIAARANALPPGLGLALAGWTAFAAGI
                     LIKGPVVVGVCAVTVLVLSLWDREWRWLGRTRPLMGLAVTLLLVLPWFVAIWLQSHGQ
                     FYQQSLGHDFATKLQSGQESHGAPPGYFLALVNLTFWPATLFLLPGLLGAIRSRAEPA
                     MRFLLVWAGACWVLFELVPTKLPHYVLPVYPALAMMAAVWVLGAKDGKPLWDRVLFHA
                     AWVQFALGAAVLAAAAAVVPGLYGEGVLWWQIALIVVFAGFGLAALVAFLRRTPLAAA
                     ACALGAALVFYPTLTLALAPRLDQLWVSPRAAAAAKELSQPGDPPVALAGYIEPSLVF
                     LLGTGTHQTNGRGAAEVGMAQGGLALVEDREQSSFLAHLAELDADATQMGEVSGFNYS
                     RGRRVHIRIYRVAPVHEEMLPPDE"
BASE COUNT          890 a         1736 c         1689 g          771 t
ORIGIN      
        1 tggcgctgta gccgacgatc cgcggccggc cggagggcca atcctcgaag gtcatctcgg
       61 ccatgggcag gatgcgcggg ccgtcctttt cctgaatcag gccgatgttg aactcgcggc
      121 cttcgacata ggcttccgcg aaccagcgcc cgccgaagcg cttggtgctg tccgcggcgc
      181 gcgcccgcac ctctgccccc ttcaccacgc aggcgtcgtc gaggccgacg gaggaatcct
      241 cgtcggccgc cttgacgata taggcgcgag cctcgtccag cccggcccac gcaggagggg
      301 acgcccaatc cggcgtcggc agagcggcgg cgcgcaggat ggtcttggcg aaaactttat
      361 cggtcgtcgc cgcgaaatgg gccgccgtgc cgccggtgaa gcgcacgccc agccgcgcca
      421 gcatggcggg cgccagcgag gcatagatcc ccgcgccgaa caccgactcc accagattga
      481 acaccacgtc cgggcgctcg cggccgagca tcttgcccag cgccttggcg tccggccgga
      541 acggcgcttg cgccgcctcg tggccccggc ttcgcagcgc gtcttccacc gcctcggcgg
      601 cgatcagcgt gtcctgctcg tcggcgggcg cgtccggcgg cacgtcggaa tggaggacga
      661 gaacgtgcat caggaaacgc gttcttttcc tcccccgttt acgggggagg tggtccgcga
      721 aacggagcgg agggggcgag cacggtgaat gccccccttg ccgccttcgg cgacactccc
      781 cccgtgtacg gggggagaaa gaggttgcgc aagctcggga tggcgcttga ggaacgacgc
      841 catgatcagg ccgatcaggt cttggtagct gaggcccgcg tcggttgcga tctggcagag
      901 atcggaatgt ttggggcgca ggccggccag cggattgacc tcgatgaagt gcggcgcgcc
      961 cttatcatcg cagcggatgt cgatgcgccc gccgtcgcgg cagcgcagcg cccgccaggc
     1021 gcccagcgcc accttgcccg ccgcctcggc ttcgggcccg cgtgcgcaga cgatctcgac
     1081 cttctcctcc cagccggctt ccttgttctc gaagccgtag ccggcgccga cgaatttcgc
     1141 cttcggcacg atctcgctga cgccgaggat ttcggcctct tcgccggtgc cggtgatacc
     1201 gacggtgaat tcgcgcccgg agaggaaggt ctccaccagc accggctggg cgaagcgcgc
     1261 cagcaggtcc gccgccaccg cccgcaacga cgccgcgtcg gccaccttgg agcgggcgcc
     1321 cacgcccttg cccgagcctt ccgccgccgg cttgaggaac aacgggaacg gcaaggcgac
     1381 cttcgccgcg tcctcgaggc gctcgatgac ggcaaacggc gcggtcggca cgccggcgtc
     1441 gcgcaccacc cgcttggcca tcgacttgtc gagcgccagc gccagggtca acgggtcgga
     1501 gaacacataa gggatgtcgt aggcgtccag cagcgcgggc gcctgagcct cgcgcgccac
     1561 gcctttcagg ccctcgcaga aattgaacac cgcgtcccag cgctcgcccg ccaccagccg
     1621 ttcggccagt ttgcggacgc cgccgatgcg caccggctcg tggcccagcc cctccagcgc
     1681 ctcgcagatg gcggcgatgg tgatctcgct gtcgaactcg gccgtctcct ccaggctgaa
     1741 gcctagcgcg agatagtcgg cccggaggtc gtaggttaca ccgatgcgca tattaagcgc
     1801 ccccactgca aagccggaaa acccgtcccc acccttcgac aagctcaggg tgaaggaatt
     1861 ccaaaatccc tcatggtgag cttgtcgaac catgagggat cgctatttcg ccgccggtta
     1921 ttccttgccg cgccccagcg tgccgccggg atcgggatag cgatagacgc cgccctcgaa
     1981 attgcgcagc aggaggtcgt cgccgtcgcg gccgaccacc ggatcgggcg cgagctggat
     2041 cttgcccccg ccccccggcg cgtcgatgca gaacatcggc gtggcatagc cggtggtgtg
     2101 gccgcgcagg ctggcgatga tctccagacc cttctcgacc gtggtgcgga aatgggccga
     2161 gccgcggatc ggatcgcact ggtagaggta atagggcttc acccggcgct tcaacaggcc
     2221 ctgcatcagc ggcaccatca ccgccgggct gtcgttgatg cccttcagca gcaccgtctg
     2281 ggagcccagc ggaatgccgg catcggccag ccgcgcggtg gactccgtca cctcctcggt
     2341 cagctcgact ggatgggtga agtggatgct catccacagc gggtgatgct tcttcagcat
     2401 cttgacgaaa tccttggtga tgcgctgcgg caacaccacg ggaatcttcg taccgatgcg
     2461 gaggaactcg atatgtttga tcgccctgag ccgggtcagc agccagtcga gcttgtcgtc
     2521 gccgatggtc agcggatcgc cgcccgacag cagcacgtcg cgcacttcgg tatggacttc
     2581 gagataggcc agcgccttgt cccattggcg ggtcgagaac tggtattcgc cgcccgggtt
     2641 gccgaccacg cgcgcgcgcg tgcaatagcg gcaataggtc gagcaggttc ccgtggtcag
     2701 gaacagcacg cggtcgggat agcggtggac cagcccgggg accaccgtgt cgtggtcctc
     2761 gccgagcgga tcgtcggcct cgccggggcc gcggacatat tcgccgccgg tcatgatgtg
     2821 ggtgcggcgc agcggctcgc tgggatcgct aaggcccatc aggctggcgt aataaggggt
     2881 aataccgacg ggcaaagcgc cttcgtggcg ctcgaccgct tggcgctcgt ccgccgacag
     2941 ggcgaagatc cgcgccagtt cgtcgagcgt gcggatgcgg ctgcgcatct gccagcgcca
     3001 atcgttccac tccagcgacg acgtgttcgg gtagaagcgg cgatagaagg cgcgggtttc
     3061 aggggttacg gggaaacgaa gcgcggctct tgccgccgct agactcgtga ccttgttcgt
     3121 ttcctggagc gaagaggcta aggccggtct tggtcggacg ggccgtagcg gaggcttcag
     3181 ctcgcgcccc cgcattgctt ggagactcat ctcagtctca tcacgctccg aaggttgccg
     3241 ccggcgcgga agcccctgtg acttcctatc agccggcaca acagacgagt cgatctttag
     3301 tagcttccat aacttcgtca agagaaagca cgcgagtact tcaactgtcg gcgaatgtca
     3361 cagacgaggt ttccggttgc cgccccccat cattgaagac tgcaaaagac gtcgcctcgt
     3421 ctctttgccg gggggcaaga gaggccgcag gaattaggac gaagggtttc ggagaacagg
     3481 cgcccggctg ccttgcggcg aacttcagcg gacttttcgc cagcgttgcc cggcgcgctg
     3541 cggcggcatc cgcagattat cccgcgcgcg ccgatgactg gccgcgccgc gcgcgaaggc
     3601 cgccgggaga ttgttgtgag tgccgggccg cagccgcatt cgcgcccggc ggggcggcgt
     3661 cattcgtccg gcggcagcat ctcttcgtgc accggcgcga cgcgatagat tcggatgtgg
     3721 acgcgccggc cgcgtgaata gttgaagccg gagacctcgc ccatttgggt cgcatcggcg
     3781 tccagttcgg ccagatgcgc caggaacgaa gactgctcgc ggtcctcgac caacgccagg
     3841 ccgccctgcg ccatgcccac ctcggccgca ccgcgtccat tggtttggtg cgtcccggtt
     3901 ccgagcagga agaccagact cggctcgata tagccggcca gcgccaccgg aggatcgccg
     3961 ggttgggaga gttcttttgc cgccgccgcc gcacgcgggc tgacccagag ctgatcgagg
     4021 cgcggcgcca gagcgagcgt cagcgtcgga tagaagacca gcgccgcccc caaggcgcag
     4081 gcggcggcgg ccagaggcgt ccggcgcagg aacgccacca gcgccgcaag gccaaagccg
     4141 gcgaagacga cgatgagcgc gatctgccac cacagcactc cctcgccata caatccggga
     4201 acgacggccg ccgccgcggc gagcacggcc gcaccgagcg cgaattgaac ccaagcggcg
     4261 tggaacagca cgcggtccca cagcggcttg ccgtctttcg cgcccagcac ccagacggca
     4321 gccatcatcg ccagcgccgg atagaccggc aggacgtaat gcggcagctt ggtcggcacc
     4381 agctcgaaca gcacccagca ggctcccgcc caaaccagca gaaaacgcat cgccggctcc
     4441 gcgcggctgc ggatcgcacc caagaggccg ggcaacagga acagggtcgc cggccagaag
     4501 gtgagattga ccagcgccag gaaatagccg ggaggcgcgc cgtgcgattc ctgtccgctc
     4561 tgaagcttgg tcgcaaaatc gtgacccagc gattgctgat agaactgtcc gtggctttgc
     4621 agccagatgg cgacgaacca gggcagcacc agcagcagag tgaccgccaa tcccatcagc
     4681 ggcctggtgc gtccgagcca gcgccattcg cgatcccaga gggaaagaac gagaaccgta
     4741 acggcgcata ccccgacgac gaccggccct ttgatcagaa ttcccgccgc gaacgccgtc
     4801 cagcccgcca gcgccagtcc caggccgggg ggcaatgcgt tcgcccgcgc cgctatgtag
     4861 actcgcagca ggaagccctg cgcccccagc acgcaggcca gcagcacggc gtcggtggtg
     4921 gcgatggtcg cttcgccgcc aagcagcagc gtgcctccca gaagcgccgc ggcgagcagg
     4981 gcgacctccg cgccggcgaa agcgccggca caccagaagg caagccagac ggcggcaacg
     5041 gcgccgatca gcgaaggcag acgataggtc cagatctgat cgcgct
//
LOCUS       DASWPU010000196         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_56773,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000196 DASWPU010000000
VERSION     DASWPU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56773"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="VGC27_04315"
     CDS             <1..1519
                     /locus_tag="VGC27_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEY0281832.1"
                     /translation="LSSFGFDHLGRMAWRQYPAGASADKVAYSWSPRDELLSLSHTLT
                     GGNSVGFTNTYSPARQIVAASLSNAAYQYVAACGGSDSYNPVNGHNQYTQITLACGGA
                     AVIDHDLNGNLTTDQSSYYQYDPENRLMKATTAAMTAAYAYDPLGRRTTKTVTGGTYA
                     GTTYFLSSGTDEIAEYDVSGALLRRYVPGPGVDQPIAMVTAAGVKTFFHQDKAGSVIA
                     MSNADGSLAEGPYTYDAYGKCRVGGQECSGGVPYKYTGRRLDPETGLYYYRARYYSST
                     IGRFLQTDPVGYTDDINLYAYVGNDPTNSTDPSGLTPQTANSNAPEVSSACSRIGAAS
                     CSGSYAGDALGTMLSEKRDTNGAEVAYLERGRDFTQEEGQRVVKQAATWKGTPYVYGG
                     KSKKGADCSGSTYCIFNEAGFPYAYTTSSKFKDAALHNPNFPFRALRSGENPQPGDVI
                     LLPGHLAIYAGSEGGHEVMWTATHPDGPNYKMEIIKYWGGTPTYFRPQVPPMGPG"
     gene            1529..1948
                     /locus_tag="VGC27_04320"
     CDS             1529..1948
                     /locus_tag="VGC27_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281833.1"
                     /translation="MSITKVVQLCSVLLVIASVGANAEARNPCGGVDRGLPAKYKAEL
                     EPALAKQLRVPKADVLEMMRDGKWSVIYVDSHDADEAFLFYAGDPLRTHYVSAWSGAA
                     GRNEELAIKRWVTLNVKGVPVRLARCFAWHVTQDRTQ"
BASE COUNT          501 a          690 c          754 g          482 t
ORIGIN      
        1 gctgtcgagc ttcgggtttg accatctcgg ccgtatggcc tggcgccagt atcccgccgg
       61 agccagcgcc gacaaggtgg cctattcctg gtcgccgcga gacgagttgc tcagcctttc
      121 gcacacgctg acgggcggaa actcggtagg gttcaccaac acctactctc cggcccgaca
      181 gatcgtggcc gcgtcgctct cgaatgcggc atatcagtat gtggcggcct gcggcgggag
      241 cgacagctac aatccggtca atggccacaa ccagtacacg cagatcacgc tggcctgcgg
      301 cggcgcggcc gtcatcgatc atgacctcaa cggcaatctg acgacggacc aaagctcgta
      361 ttaccagtac gatcccgaga accggttgat gaaagcgacg accgccgcca tgacggcggc
      421 ctatgcctac gatccgctag ggcggcggac cacgaaaacc gtcacaggcg ggacctatgc
      481 ggggacgacc tacttcctca gttcgggcac cgacgagatc gcggaatacg acgtcagcgg
      541 cgcgctgctg cgccgctatg ttcccggccc gggcgtcgac cagcccatcg cgatggtgac
      601 ggcggcgggg gtcaagacct tcttccacca ggacaaggcg ggcagcgtga ttgcgatgag
      661 caatgcggac ggctcgctgg cggaaggccc ctacacctat gacgcctatg gcaaatgccg
      721 cgtgggcggc caagaatgca gcggcggagt gccctacaaa tacaccgggc ggcgtctcga
      781 cccggagacc gggctctatt attaccgcgc gcgctactac tcctccacca tcggccgctt
      841 cctgcagacc gatccggtgg gctacacgga tgatatcaac ctctacgcct atgtcggaaa
      901 tgatccgaca aactcaactg atccgagtgg tctcacacca caaacggcga actcaaatgc
      961 accggaggtt agtagtgctt gttcgagaat tggggcagca agttgcagcg gttcatatgc
     1021 aggcgatgcc cttggcacaa tgcttagcga aaagcgtgac acaaatggag cggaagtggc
     1081 ctacctggag cgggggcgtg attttacgca ggaggaagga cagagggttg taaagcaagc
     1141 tgctacgtgg aaaggcacgc cttacgtata tggggggaaa tcgaagaagg gcgccgattg
     1201 ttctgggagc acgtattgca tctttaacga agcgggattc ccgtatgcct acacaacgtc
     1261 gtccaagttt aaggatgctg cacttcacaa tccaaatttc ccgtttcgtg ctcttcggtc
     1321 cggcgagaat cctcagccgg gagacgtgat cctgttgcca ggtcatttag caatttatgc
     1381 gggatcggaa ggtggtcatg aggtgatgtg gacggccacg cacccggacg gcccgaacta
     1441 taaaatggaa ataatcaagt attggggcgg gacgccgacc tatttccgac cgcaagttcc
     1501 tccgatggga ccaggataag gtgctgcaat gtcgatcaca aaggtagtgc aattgtgctc
     1561 tgttctcttg gtcattgctt ctgttggggc gaacgcagag gctcgtaatc cgtgcggagg
     1621 tgtcgataga ggcctgcctg ccaagtacaa agcagagttg gaaccggctc tagcgaagca
     1681 actgagggtt ccaaaggcgg atgttcttga gatgatgcgg gatggcaaat ggagcgtgat
     1741 ctacgtcgat agccatgacg cagatgaagc atttttgttt tatgccggag accctcttcg
     1801 cacccattac gtgagcgcat ggagcggcgc agcaggtcgg aatgaggagc tagccatcaa
     1861 aagatgggtg accctgaacg tcaagggtgt tcccgtgcgc ctcgcccgct gcttcgcttg
     1921 gcatgtgaca caagatcgta ctcagtaacc ggcagtcgat ctcgcatgcg cttccttagc
     1981 cgccgcaccg ttgtctcgta ccagcgtgcg ccgcagcagg ttgcgtgccc gactcgcgat
     2041 gaatccgttc cgctgctcac gctgaatcgc gtcggcatcg gtgctccgcc gtcgtctttt
     2101 tgatcgccgg actgcatcgt gcgcagtgat gttaccaccg tccggctgca cagattttgc
     2161 tcgtgtgatg gtgtccggca cgttgctggc gacccgaatc cgttgcggat tttgctgtgg
     2221 aagcgacggt gaactgcccc cgaatctgtc cgtggccgag tgatccgggg caaattttga
     2281 tcgtgaatcg gtgcgggaac ggtgctccgg cgccgccagc gcggtgcgct acattgtgtc
     2341 cgaccatctg ggctccacgg ccgtcgtcac cgacgagagc ggggcggtgg tggagcgcaa
     2401 tgcctacgat ccctggggca ggcggcg
//
LOCUS       DASWPU010000197         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_32420,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000197 DASWPU010000000
VERSION     DASWPU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32420"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /gene="ilvB"
                     /locus_tag="VGC27_04325"
     CDS             complement(<1..1470)
                     /gene="ilvB"
                     /locus_tag="VGC27_04325"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEY0281834.1"
                     /translation="MDKDETNPSQIAAPAIEMTGAEMVIRALKDQGVTHIFGYPGGMV
                     LPIYDALFAANDIVHVLVRHEQGATHAAEGFARSTGKPGVVLVTSGPGVTNAVTGITD
                     ALMDSIPLVVLTGQVFTNLIGNDAFQECDTVGITRPCTKHNWLVKDVGELSRVLHEAF
                     QIATTGRPGPVVVDIPKDIQFKKGPYVGPEKVAHRTYRPRTEPEAGVIEEAVALMAAA
                     KRPIFYTGGGIINAGPAASAGLRELVALTGFPITSTLMGLGAYPASGKNWLGLLGMHG
                     TYEANNAMHDCDLMICLGARFDDRVTGRTDFFSPGSKKIHLDIDASQINKNIRVDLPV
                     VADAAKAIAAMVKVWKARKYKANGAALKEWWEQIERWRGKECLAYKPSSELIKPQYAI
                     DRLYELTKGRDLYITTEVGQHQMWAAQRFKFEEPNRWMTSGGLGTMGYGLPAAVGVQI
                     AHPGSLVVDIAGEASVQMTMQEMATAVQYNLPIKIFILNN"
BASE COUNT          318 a          522 c          534 g          333 t
ORIGIN      
        1 gttgttgagg atgaaaatct tgatcggcag attgtactgc accgccgtgg ccatttcctg
       61 catcgtcatc tgcacgctgg cctcgccggc gatatcgacg accagactgc cgggatgggc
      121 gatctgtacg ccgacggcgg cgggcaagcc gtagcccatg gtgcccagcc cgccggaggt
      181 catccagcgg ttcggctcct cgaacttgaa gcgctgcgcc gcccacatct gatgctggcc
      241 gacctcggtg gtgatgtaga gatcgcggcc cttcgtcaac tcgtaaagcc ggtcgatggc
      301 atattgcggc ttgatcagct cggacgacgg cttgtaggcg aggcactctt tgccccgcca
      361 gcgctcgatc tgttcccacc actccttcaa ggcggcgcca ttcgccttgt atttacgcgc
      421 tttccagacc ttcaccatcg ctgcgattgc cttggccgca tcggcgacga cgggaagatc
      481 gacgcggata ttcttgttga tctgcgaggc gtcgatatcg agatgaatct tcttcgagcc
      541 cggcgaaaag aagtcggtgc ggccggtgac gcgatcgtcg aagcgcgcgc ccaggcagat
      601 catcaggtcg caatcgtgca tcgcgttgtt ggcttcgtag gtaccgtgca tgcccagcag
      661 gccgagccag ttcttgcccg acgccggata ggcgccaagg cccatcagcg tggaggtgat
      721 cggaaagccg gtcagcgcga ccagctcgcg caggcctgcg ctggccgccg gcccggcgtt
      781 gatgatgccg ccgccggtgt agaagatcgg ccgcttggcc gccgccatca gcgcgaccgc
      841 ttcctcgatg acgccagctt cgggttcggt ccgcggccgg taggtgcgat gggcgacctt
      901 ttccgggccg acataaggac ctttcttgaa ctggatgtct ttggggatat cgaccacgac
      961 cggacccgga cggcccgtgg tggcgatctg gaacgcctcg tgcagcacgc gggaaagctc
     1021 gcccacatcc ttcaccagcc aattgtgctt ggtgcagggc cgggtgatgc cgaccgtatc
     1081 gcactcctga aaagcatcgt tgccgatcag gttggtgaag acctggccgg tcagcacgac
     1141 cagcggaatg gaatccatca gcgcgtcggt gatgccggtc accgcgttgg tgacgccggg
     1201 gccggaagtc accagcacca cgccgggctt gccggtggag cgcgcaaagc cttcggcggc
     1261 gtgggtcgcg ccttgctcat ggcgcacaag gacgtgaacg atgtcgttgg ccgcgaacag
     1321 cgcgtcgtag atcggcagca ccatgccgcc gggataaccg aaaatatgcg tgacgccttg
     1381 atccttcagc gcgcgtatca ccatctccgc gccggtcatc tcgatcgccg gcgcggcaat
     1441 ttgggagggg ttcgtttcat ccttgtccat cggggacatc cttcaactat gagctagaaa
     1501 agcgaaaggg gcgttgccgc ccctttggtg tcagaccgcc atgccggaaa acgggtcaac
     1561 cccgtccgga gagcggcctg ccgataagta cgagcacgtt tctcaaaaca cgacctcgtt
     1621 taacggcagg caaattacgg ggcggaagat tcgccgtcaa gcaatatcat gaggtttttt
     1681 gtcgcaatac agaaacaatg ttaccaa
//
LOCUS       DASWPU010000198         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_20215,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000198 DASWPU010000000
VERSION     DASWPU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20215"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2254)
                     /locus_tag="VGC27_04330"
     CDS             complement(<1..>2254)
                     /locus_tag="VGC27_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0281835.1"
                     /translation="GSNDFLSAVGATKGQMVQVNLTASTNLKSLDDFRNMIVKSKNGG
                     YVRLSDVANVTLGSEDYESAVTFDGRAGVFIGIKVAPTANVLDVIKGVRAAFPGIISQ
                     LPQGLNANIAYDSTKFIQSAIDEVVWTLAEAVLIVIFVIFVFLGSPRAITIPILTIPL
                     SLIGAGIFMLAFGFTINLLTLLALVLAIGLVVDDAIIVVENVSRHLEEGRTPLDAALM
                     GARELGGPIIAMALVLVAVYVPIGFIGGLTGALFVEFAFTLVGSVVISAIVALTLSPM
                     MASKLLRSHAATRSAWERKLVVFIDEKFDILHTKYQHLLHKSLNELAVIVVFAAVVLA
                     SIYFLFSMAKSELSPPEDQGFVIALLTPSPTATLQQSQIWQREVYSVARSFPETDHVF
                     QISAPGTNFAGVVLKPWGERSNSSDTVQAGMQKKLSVIAGVRAAAFQPPPLPGPRGLG
                     VQFVIQTTQSFQQLNEVANLVRDEALASKMFVYLDSDLKYDQPQTEVVIDRDKVASFG
                     LTMRDVGSSMTAMLGGGYVNYFSLSERAYRVIPQVEQRYRLNADQLRNYYIRTASGRN
                     IPLSTVAHLETTVVPESLNHFQQQNAATITAIPRPGLTTGEAIDGLRQIAAKVLPDGY
                     RIEYAGQTRQQVQESSALIVTFFFALLIIFLTLAALFESFRDPLTILISVPMSICGAL
                     IFIAMGVGGASLNIYTEVGLVTLIGLISKHGILIVRFANDLQLQGMSKREAIEEAAAI
                     RLRPILMTTAA"
BASE COUNT          459 a          681 c          721 g          393 t
ORIGIN      
        1 ggcggccgtg gtcatcagaa tcggccgcaa gcggatggcc gcagcctcct cgatcgcctc
       61 gcgtttgctc atgccctgca actgaagatc gttcgcgaag cggacgatca ggataccgtg
      121 cttgctgatc agcccgatca aggtcaccag gcccacctcg gtatagatgt tgaggctggc
      181 gccgccgacc cccatggcga tgaagatcaa ggcgccgcag atcgacatcg gcacgctgat
      241 caggatggtg agaggatcgc ggaagctctc gaacagcgcc gccagggtca gaaagatgat
      301 gagcagggca aaaaagaagg tgacgatcag ggcgctcgat tcttgaactt gctggcgggt
      361 ctggccggca tactcgatcc ggtagccatc aggcagcacc ttggcggcga tctggcgcag
      421 cccgtcgatc gcctcgcccg tggtcagacc cggccgcggg atcgcggtga tcgtggcggc
      481 gttctgctgc tggaaatggt tgagcgactc cggcacgacc gtggtttcca gatgcgccac
      541 cgtggacagc ggaatgttcc ggcccgaagc cgtacggatg taatagttcc tcaactggtc
      601 cgcattcagc cggtagcgct gctcgacctg cggaatgacc cggtaggcgc gctctgacag
      661 gctgaaatag ttcacgtagc cgccgcccag catggcggtc atggaactgc cgacatcgcg
      721 catggtgaga ccgaagctcg ccaccttatc ccggtcgatg acgacttcgg tctgcggctg
      781 gtcgtatttg aggtcggagt cgagatagac gaacatcttc gacgccaagg cctcgtcgcg
      841 caccaaattc gccacttcgt tcaactgctg gaacgactgt gtcgtttgaa tcacgaactg
      901 cacgccgagg ccgcgaggac ccggcagcgg cggcggctga aacgcggcgg cgcgaacccc
      961 ggcgatgacg ctcaacttct tttgcatgcc ggcctgaacc gtgtcggacg agttgctgcg
     1021 ctcgccccag ggcttgagca cgacgcccgc gaagttcgtt cccggcgcgc tgatctggaa
     1081 gacgtgatcg gtctcgggaa aagagcgggc gacgctgtac acttcgcgct gccaaatctg
     1141 cgattgttgc agggtggcgg tcggcgacgg cgtcagcaag gcgatgacaa agccctggtc
     1201 ctcgggcggc gacaactcgc tcttggccat cgagaacagg aaatagatgc tggccagcac
     1261 gaccgccgca aacaccacga tcaccgccag ctcgttcagg ctcttgtgca gcaggtgctg
     1321 gtacttggtg tgcagaatat cgaacttctc gtcgatgaag acgacaagct tgcgttccca
     1381 cgccgagcgc gtcgccgcgt gcgagcgcag cagcttgctc gccatcatgg gcgacagcgt
     1441 cagcgcgacg atcgcggaaa tgacgacgct accgaccagg gtaaatgcaa attcgacgaa
     1501 cagcgcgccg gtcaacccgc cgatgaagcc gatcggcaca tagaccgcga cgagcacgag
     1561 cgccatggcg atgatcgggc cgccgagttc gcgcgcgccc atcaaggcgg cgtcaagcgg
     1621 cgttcgcccc tcctccagat ggcggctgac gttttcgacc acgatgatcg catcgtcgac
     1681 caccagtccg atcgccagca caagcgccag cagcgtgagc agattgatgg tgaagccgaa
     1741 cgccagcatg aaaatgccgg caccgatcag cgacagggga atggtcaaaa tcggaatggt
     1801 gatcgcgcgc ggcgatccca ggaagacgaa gatgacgaaa ataacgatca ggaccgcttc
     1861 ggccagcgtc cagaccactt cgtcgattgc gctttggatg aacttggtcg agtcgtaggc
     1921 gatgttggcg ttcaacccct gcggcaactg gctgatgatg ccggggaacg ccgcgcgcac
     1981 gcccttgatg acgtcgagca cgttggcggt gggcgcgacc ttgatgccaa tgaagacgcc
     2041 cgctctgccg tcgaaggtga ccgccgactc gtagtcctcg gagcccagcg tgacgttggc
     2101 gacgtcggag agccggacat agccgccgtt cttggacttg acgatcatat tgcgaaaatc
     2161 gtcgagcgac ttgagattgg tggaggccgt caggttgacc tgaaccatct gccctttggt
     2221 cgcgccgacc gccgacagga agtcgttgct gccg
//
LOCUS       DASWPU010000199         7680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_86990,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000199 DASWPU010000000
VERSION     DASWPU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86990"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..676)
                     /locus_tag="VGC27_04335"
     CDS             complement(86..676)
                     /locus_tag="VGC27_04335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0281836.1"
                     /translation="MLIALIRHGPTEWNAQKRVQGRIDRPLSAAGQARMAKLLPPAGF
                     ENARPFCSPQLRARQTASLLGLENPTLDERLREQNWGEWEGLTRAQMLARDGADAFER
                     AGRGLRFRPPGGESTSELQARVASFFADAAKLPQDAVAVTHMGVLRSAYALATGWDMA
                     APMPDELDLAAALVLSLDVRGKPAIAELNAPLRARK"
     gene            complement(712..1506)
                     /gene="xth"
                     /locus_tag="VGC27_04340"
     CDS             complement(712..1506)
                     /gene="xth"
                     /locus_tag="VGC27_04340"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0281837.1"
                     /translation="MKIATWNINSVRLRIDLAARLLREHGPDVLCLQEIKVAGELFPK
                     EAFAKLGYVHQAINGQKAYHGVAILSRLPFKKIAAEDFCREGHARHISAVFENGAELH
                     NFYVPAGADIPDPEINPKFAHKLDFLRNMARFFEKRRAKRNTPVILVGDLNVAPLEND
                     VWSHKQLLDVVSHTPVETTLLGEAMVAFDWTDATRAFIPSTEKIYSWWSYRAADWEAS
                     DRGRRLDHVWVSPALKGGLVSQSIARKMRGWAKASDHVPVIAELKI"
     gene            complement(1503..2555)
                     /gene="tsaD"
                     /locus_tag="VGC27_04345"
     CDS             complement(1503..2555)
                     /gene="tsaD"
                     /locus_tag="VGC27_04345"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY0281838.1"
                     /translation="MRAFTVLGIETSCDETAAAVVRGAGCGPAAHAGPGEILSNIIFS
                     QLEEHRPYGGVVPEIAARAHVEMLDGIVERALAEAGLKLGEVDAVAATAGPGLIGGVM
                     VGLVTAKALALAAGKSLIAVNHLEAHGLSARLSEGLEFPFLLLLVSGGHCQLLGVEGV
                     GRFRLYGTTIDDAAGEAFDKTAKLLGLAYPGGPRIEALAREGNPKRYKLPRPLAGREG
                     ADFSFSGLKTAVRQVTLEGDVQAADLAASFQAAVIDILRDRTKAAMAMFVRDFPQTAS
                     PAFVMGGGVAANGAIRAALGALASAEGFALKVPPPALCTDNAAMIAWAGIERAGLGLF
                     DPLNVAARARWPLESL"
     gene            2604..3530
                     /gene="hemC"
                     /locus_tag="VGC27_04350"
     CDS             2604..3530
                     /gene="hemC"
                     /locus_tag="VGC27_04350"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY0281839.1"
                     /translation="MSQSSPLRLGTRGSRLGRIQAGMVARELRQKGTACEIVAVKTTG
                     DRILDRLLADAGGKGLFTKELEEALLSGAIDLAVHSMKDVPVALPEDLTIAAILPRED
                     PSDVLIAKRAPTLWQLPAGARVGTSSVRRKAQILRARPDLECVLLRGNVDTRLAKLEA
                     GEIDAVLLALAGLKRLGLAERVTAVLNPKEWLPSLAQGAVGIEIRRSDMRTAALVGAL
                     DDEAAAVELACERAFQAALDGSCRTPIAGLARIEDKRLSFRGEVIAPDGSDFAATDFQ
                     IALGSDALQEAARAGREAGMALLPRAARWLEI"
     gene            complement(3552..3968)
                     /locus_tag="VGC27_04355"
     CDS             complement(3552..3968)
                     /locus_tag="VGC27_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0281840.1"
                     /translation="MPKKQANPIDAQVGNRLRLRRMMIGMSQERLGELLGLTFQQVQK
                     YEKGVNRIGAGRLFEIARILGVPIYYFYESVSEQLAGAPGFAETDTPPVLEFVSSGEG
                     LQLSLAYMRIKDPKVRKRVLDLVKSLSEDVAAKPEY"
     gene            complement(4066..5622)
                     /gene="lnt"
                     /locus_tag="VGC27_04360"
     CDS             complement(4066..5622)
                     /gene="lnt"
                     /locus_tag="VGC27_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY0281841.1"
                     /translation="MFSAFARVGAFVRGLHGGRRFFFAWVAGLLSALGFAPFGFFPFL
                     LLGYGALVLLIDGAQGASRPLRASALAGFAFGFGQFLAGLHWIGYAFMVDPAAHLWQI
                     PFVAVLVPGGLALFIALACAAAAMFWREGPARIFVFAVALAIAEWLRGHLLTGFPWNI
                     AAYGWGASLGVLQSAALIGSYALTLLTILFGASLAELFHARPKWLLPFSLAALFILVW
                     IGGEVRLAAAPTVPVPGVRLRLVQPGIPQAEKYMQRYVVRNWERLLSLSTKPAASSPT
                     IIVWPEAAPPFLLAQEPAALDQIAMLTAHRRVLLTGAIRRERNGSDEPRYYNSFYLFG
                     RGGELLASHDKFHLVPFGEYLPFERTLGRWGIDKLTGISGSFAGGDGPHSYDVPEAPS
                     VGPLICYEILFPNAVIGASRPGWFANVTDDSWFGPWAGPRQHLLVARVRAIEEGIPVA
                     RAANTGISAIIDPLGRITAKLDLDRVGVVDGELPAAIAPTPYSRLRGVWFILFLSAFT
                     GLAWGLNGRK"
     gene            complement(5626..6549)
                     /locus_tag="VGC27_04365"
     CDS             complement(5626..6549)
                     /locus_tag="VGC27_04365"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0281842.1"
                     /translation="MSEPNRAVLDGEEEKAPSFLKRMAQLLRGDNAAAAIRESLEEVI
                     EESDRQSAALSAPERTMLANLLKFGELQVKDVMVPRADIVAVEEKTSLADLILLFREA
                     QHSRLPVYRETLDDPTGLVHLKSVLELLESDGGRDFRLREVPIAKIKHDILFAPPSMP
                     VLELLLKMETSHTHLALVIDEYGGTDGLVSIGDLIEEIVGDIADEHDEEAPHARAFAG
                     GGFVTDARIALEDFKSETGIEFPLDETQGEIDTLGGLVVSLLGRVPQRGEIVAHPAGY
                     EFEVLEADPRRVKRLRLRPSAPPAAEKRDAD"
     gene            complement(6554..7012)
                     /gene="ybeY"
                     /locus_tag="VGC27_04370"
     CDS             complement(6554..7012)
                     /gene="ybeY"
                     /locus_tag="VGC27_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY0281843.1"
                     /translation="MSITLLILETKWRSARGLGARLKRAAAEALAQGGGETDAALTIL
                     LTSDRHVHSLNLGFRSEDRPTNVLSFPSSMGAKGYLGDIAIAYGVTATEAKGAGKSLI
                     DHATHLAVHGVLHLIGFDHATARQAKIMESLETRVLGALNVADPYRQEVA"
     gene            complement(7009..7365)
                     /locus_tag="VGC27_04375"
     CDS             complement(7009..7365)
                     /locus_tag="VGC27_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY0281844.1"
                     /translation="MRGRTLSRAFVILDEAQNCTAAQMKMFLTRLGEESRMVVTGDPS
                     QSDLPGGRPEGLAQAVSILGDIEGAAVARFSEKDVVRHALVTRIVTAYNAAEQGASAH
                     RVRKRALRKDEDKDGS"
     gene            complement(7293..>7680)
                     /locus_tag="VGC27_04380"
     CDS             complement(7293..>7680)
                     /locus_tag="VGC27_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY0281845.1"
                     /translation="PAQAAYLDLMRTHPLVFGIGPAGTGKTYLAAAFGAHLLHERRVE
                     RLILSRPALEAGERLGFLPGDLKEKIDPYLRPLYDALFGAMGESCLKLMEQASSKSLR
                     SPSCAGVRSAAPSSFWTRRRIAPRRR"
BASE COUNT         1440 a         2490 c         2426 g         1324 t
ORIGIN      
        1 ccgatcctgg cgaagcttgc aagatcgtgg atgtgtcacc tgggtggccc gcattcgcgg
       61 gccatgacat tgatatcttt atcttctact ttcgcgccct cagcggcgca ttgagttcgg
      121 cgatcgccgg cttcccgcgc acgtcgagcg aaagcaccag tgctgcggcc aggtccagtt
      181 cgtccggcat cggcgcggcc atgtcccagc ccgtcgccag cgcataggcc gagcgcagca
      241 cgcccatatg agttaccgcg acggcatctt gcggcagctt cgccgcgtcg gcgaaaaaac
      301 ttgccacccg cgcctgcagc tcgcttgtcg attcgcctcc cggcggacgg aagcgaaggc
      361 cgcgcccggc gcgctcgaag gcgtcggcac cgtcgcgcgc cagcatctgc gcgcgcgtca
      421 gcccttccca ttcgccccaa ttctgttcgc gcagccgttc gtcgagcgtc gggttttcca
      481 gccccagcag cgaagccgtc tgacgggcgc gcaattgcgg actgcaaaag ggacgcgcgt
      541 tctcgaaccc ggccggcggc aaaagctttg ccatcctggc ttggcccgcg gcgctgagcg
      601 gcctgtcgat gcggccttgg acgcgctttt gcgcgttcca ttccgtgggt ccgtggcgga
      661 tgagggcgat cagcaagtgc ctgttcctca ttgtggcgag cggcggcgaa atcagatctt
      721 caattccgcg atcaccggca catggtcgga ggccttggcc cagccgcgca tcttgcgggc
      781 gatggactgc gaaaccagcc cgcccttcag cgccgggctg acccagacat ggtcgagccg
      841 gcggccgcgg tcggaagcct cccagtcggc ggcgcgatag ctccaccacg aatagatttt
      901 ctccgtagac ggaataaatg cgcgcgtcgc gtcggtccag tcgaaggcga ccatggcctc
      961 gccgagcaaa gtggtctcga ccggcgtgtg gctgacgacg tcgagcaact gcttgtggct
     1021 ccacacgtcg ttctccaagg gcgcgacgtt gagatcgccg accaggatga ccggcgtatt
     1081 gcgcttggcc ctgcgcttct cgaagaagcg cgccatgttg cgcaggaagt cgagcttgtg
     1141 ggcgaatttg ggattgatct cgggatcggg aatgtcggcc ccggcgggga cgtagaaatt
     1201 atgcagctcg gcgccgttct cgaagacggc gctgatatgg cgggcatggc cttcgcggca
     1261 gaaatcctcc gccgcaatct tcttgaacgg caaacgcgaa aggatcgcca cgccgtgata
     1321 ggccttctgc ccgttgatcg cctgatggac atagcccagt ttggcgaagg cttccttcgg
     1381 gaacaattca ccggcgactt tgatctcttg caggcacaac acgtcggggc cgtgctcgcg
     1441 caacaggcgc gccgccagat cgatccgcag ccgcaccgaa ttgatgttcc aggtggcgat
     1501 cttcataggg actccaaagg ccagcgcgcc cttgcggcga cgttcagcgg atcgaacaga
     1561 ccgagcccgg cccgttcgat ccccgcccag gcgatcatgg cggcgttgtc ggtgcagagc
     1621 gcgggcggcg gaaccttcag cgcgaatccc tcggcggacg cgagcgcgcc gagcgcggcc
     1681 cggatggcgc cgttggcggc gacgcctcct cccatgacaa aagcggggga tgccgtttga
     1741 ggaaaatcgc ggacgaacat cgccatcgcg gccttggtgc ggtcgcgcaa gatatcgatc
     1801 accgccgcct ggaacgaggc cgccagatcg gccgcttgca catctccctc aagcgtcact
     1861 tgccgcacgg cggtcttaag gcccgaaaag gaaaagtcgg cgccttcgcg cccggcgagc
     1921 ggcctgggca atttgtagcg cttgggattg ccttcgcgcg ccaaggcttc gatgcgcggc
     1981 ccgcccggat aggcaaggcc cagaagtttg gccgtcttgt cgaaggcttc gccggccgcg
     2041 tcgtcgatcg tcgttccgta gagccggaag cggccgacgc cttccacgcc gagaagctgg
     2101 caatggccgc ccgagaccaa gagcagcagg aagggaaatt ccaatccttc gctgagccgg
     2161 gcgctgaggc cgtgggcttc cagatgattg accgcgatca gcgatttgcc tgccgccagc
     2221 gccagcgcct tggcggtgac cagccccacc atcactccac cgatcaggcc ggggccggcg
     2281 gtggcggcga cggcatcgac ctcgccgagc ttcaatccgg cctcggccag cgcccgctcg
     2341 acgatcccgt ccagcatttc cacatgggcg cgcgcggcga tttcgggaac gaccccgccg
     2401 taaggccggt gttcctccaa ctggctgaag atgatgttgg agaggatttc gccgggccca
     2461 gcgtgggccg cgggcccgca gcccgcgccg cgcaccaccg ccgccgcggt ttcgtcgcag
     2521 ctcgtctcga tccccagaac cgtgaatgcg cgcaaaagca aaacccgtga taaggaggaa
     2581 caggctctaa cccgaaaacg cccatgtcgc aaagctctcc ccttcgcctt ggcacccgcg
     2641 gctcccggct cggccgcatc caggccggca tggtcgcgcg cgaattgcgg caaaaaggaa
     2701 ccgcttgcga gatcgtagcg gtcaagacca cgggcgaccg cattctcgac cgtctcttgg
     2761 ccgatgccgg gggcaagggc ctgttcacca aggaactgga agaggcgctg ctttccggcg
     2821 ccatcgatct tgccgtccat tccatgaagg acgtgccggt ggcgctgccc gaggatttga
     2881 ctatcgccgc gatcctgccg cgcgaggatc ccagcgacgt ccttatcgcc aaacgcgcgc
     2941 ccacgctgtg gcaattgccg gccggggcac gggtcggaac cagttcggtg cggcgcaaag
     3001 cgcaaatcct tcgcgccagg ccggacctcg aatgcgtgct gttgcgcggc aatgtcgata
     3061 ctcgcctggc caagctggag gccggcgaga tcgatgcggt cctgctggcc ctggccgggt
     3121 tgaagcgcct ggggctggca gagcgcgtca ccgccgttct gaaccccaaa gagtggctgc
     3181 cgtcgctggc gcaaggcgca gtgggaatcg aaatccgccg ctccgatatg cgcaccgctg
     3241 cgctggtcgg cgcgctggac gacgaagccg cagcggtgga actggcgtgc gaacgcgctt
     3301 tccaggcggc gctcgacggc tcgtgccgca cgccgattgc gggcctggcc cgcatcgagg
     3361 acaagcgcct ttcctttcgc ggcgaagtca tcgccccgga cggcagcgat ttcgcggcga
     3421 cggattttca aatcgcgctt ggttccgatg cgctgcaaga ggcagcgcgc gccggacgcg
     3481 aagcgggcat ggccctgctt cctcgcgcag cgcgctggct tgaaatctaa gttctggtgg
     3541 aagaaacgac gttaatattc cggcttcgcc gctacgtctt cggacaggct cttgaccaga
     3601 tcgaggacgc gcttgcgcac tttgggatcc ttgatgcgca tataggcaag cgaaagttgc
     3661 aagccttcgc cgctcgatac gaattcgaga accggcgggg tgtccgtttc ggcaaagccg
     3721 ggtgcgccgg ccagctgttc ggagacgctt tcgtaaaaat aatagatcgg tacgccaagg
     3781 atgcgtgcaa tttcgaaaag acggcccgca cctatgcggt tgacgccttt ctcgtatttc
     3841 tgcacctgct ggaaggtcag gccgagcagt tcgcccagcc gttcctggct catgccaatc
     3901 atcatccgcc gcaggcgcag ccggtttccg acctgagcat cgattggatt agcttgtttt
     3961 tttggcacgg ccggtaacag ctatatttat tcgttggcgg cgaggacctt atccgtacaa
     4021 ctaccgaaac ggaaagatta aaacaccgat tttactcatc atgcgtcatt ttcttccatt
     4081 cagcccccaa gccagacccg taaaggcgct taggaataga ataaaccata cccctcgcag
     4141 gcgagaatag ggggtaggtg caatcgctgc gggaagctcg ccgtccacaa caccaacccg
     4201 atcaagatca agcttcgccg tgattcgccc caaagggtcg atgatggccg aaatacccgt
     4261 attggcggcg cgcgccaccg gtatgccctc ttcgatggcg cgtacccgcg ccaccaagag
     4321 atgctgccgc ggccccgccc aaggcccgaa ccacgaatca tcggtgacgt tggcaaacca
     4381 gcccggcctg cttgcgccta tgacggcgtt ggggaagaga atctcgtagc agatcaaggg
     4441 gcctacgctt ggcgcctcgg gcacatcgta gctgtgcggc ccatccccgc cggcaaaact
     4501 gccgctaatc cccgtgagct tgtcgatgcc ccagcggccg agggtgcgct cgaagggcag
     4561 atactcgccg aagggaacca aatggaattt gtcgtggctg gccaacagtt cgccgccccg
     4621 gccgaaaaga tagaagctgt tgtaatagcg cggctcgtcc gatccgttgc gttcccggcg
     4681 aatggccccg gtcagaagca cgcgccggtg cgcggtcagc atggcgatct gatccagcgc
     4741 cgcgggctct tgcgccaaca ggaacggcgg cgccgcctcg ggccagacga tgatcgtcgg
     4801 ggaagacgcg gccggctttg tactcagtga cagaagccgt tcccagtttc tgacgacgta
     4861 acgctgcatg tacttttcgg cttgcggaat accgggctgc accagccgca gccttacgcc
     4921 cggcacaggg acagtcggcg ccgccgcaag cctgacctcc ccgccaatcc agactaggat
     4981 gaaaagggcg gccagggaga aaggaaggag ccatttcggc cgggcgtgga acagttcggc
     5041 gagagacgcg ccgaatagaa tcgtcagcag ggtcagcgca taagagccga tcaatgccgc
     5101 gctttgcagg accccgagcg aagcgcccca gccataggcc gcgatgttcc agggaaagcc
     5161 cgtgagcagg tgaccgcgca gccattcggc gattgccaac gccacggcga aaacaaaaat
     5221 tcgcgccggg ccttcgcgcc agaacatggc ggccgcggcg caagccaggg cgatgaaaag
     5281 cgcaagcccg ccgggaacca ggaccgcaac gaagggaatc tgccataggt gcgcagccgg
     5341 atcgaccatg aaggcataac cgatccaatg caggcccgcg aggaactgtc cgaaaccgaa
     5401 ggcaaaaccg gcaagcgccg aagcccggag gggacgggag gcgccttgtg ccccgtcgat
     5461 cagcagaaca agcgcgccat atcccagcag cagaaacgga aagaatccga agggcgcaaa
     5521 ccccaatgcc gacaacagcc ccgcgaccca cgcgaaaaaa aagcgccggc ctccgtgcaa
     5581 gccgcgcacg aacgccccca cccttgcgaa agcggaaaac atcgttcagt ccgcatccct
     5641 tttttcggcg gcgggcggcg cggacgggcg caggcgcagg cgtttgactc ttctgggatc
     5701 ggcttcgagg acttcgaatt catagccggc gggatgggcg acgatttcac cgcgttgcgg
     5761 cacgcgcccc aacagcgaaa cgaccagccc gccaagcgtg tcgatctcgc cctgggtctc
     5821 gtcgagcgga aactcgatgc cggtttcgct cttgaaatcc tccagagcga tacgggcgtc
     5881 ggtgacgaac cctcccccgg caaacgcgcg ggcatgggga gcctcctcgt cgtgctcgtc
     5941 ggcaatgtcg ccgacgatct cctcgatgag gtctccgatc gagaccagcc cgtcggtgcc
     6001 gccatattcg tcgatcacca gcgccagatg ggtatgcgag gtctccatct tcagaagcaa
     6061 ttccagaacg ggcatggacg gcggcgcgaa caggatgtcg tgctttatct tcgcgatagg
     6121 cacttcgcgc agacggaagt cccggccgcc gtcggattcc agaagttcca gcacgctttt
     6181 cagatggacg aggcccgtgg ggtcgtcgag ggtttcgcgg tagacgggca ggcgcgaatg
     6241 ctgcgcctcg cggaacaaga gaatgagatc ggccagcgag gttttttctt ccaccgcgac
     6301 gatatcggcg cgcggcacca tgacgtcctt gacctggagc tccccgaact tgagaagatt
     6361 ggcgagcatg gtgcgttcgg gcgccgacag cgccgcactc tggcggtcgc tttcctcgat
     6421 cacctcctcc aggctttcgc ggatcgccgc cgccgcgttg tccccgcgca agagttgcgc
     6481 catgcgtttg agaaaggacg gtgctttctc ttcttcgccg tcgaggacgg cgcgatttgg
     6541 ctcgctcata acgtcaggct acctcttgcc tataaggatc ggcgacgttg agggccccaa
     6601 ggaccctggt ctcgagcgac tccatgatct tggcctggcg cgcggtggcg tgatcgaaac
     6661 cgatgaggtg cagaacgcca tgcaccgcca gatgggttgc atgatcgata aggctcttgc
     6721 cggccccttt ggcttccgtc gccgttacgc cataggcgat ggcgatatcg cccagatacc
     6781 ccttcgcccc catggacgaa ggaaacgaca gcacgttggt gggtctatcc tcgctccgga
     6841 agccgagatt caagctgtgc acatgccggt cgctcgtcag caggatggtc agcgccgcat
     6901 ccgtttctcc gccgccctgg gccagcgcct cggccgcggc gcgcttcaga cgcgcgccca
     6961 ggccgcgcgc ggatcgccat ttcgtctcca gaataaggag cgtaatgctc aactgccgtc
     7021 cttgtcttcg tccttgcgca gcgcgcgctt gcgtacgcga tgcgcgcttg cgccttgctc
     7081 cgccgcatta taggcggtga cgatgcgcgt gacgagagcg tggcggacca cgtccttttc
     7141 gctgaagcgg gccacggcgg cgccttcgat gtcgccgaga atcgacaccg cctgagcgag
     7201 accctccggc cgccctccag gcaggtcgct ttgcgaggga tcgccggtga cgaccatgcg
     7261 cgattcctcg cccagccggg tgagaaacat cttcatctgc gccgcggtgc aattctgcgc
     7321 ctcgtccaga atgacgaagg cgcggctgag cgtacgcccg cgcatgaagg cgagcggagc
     7381 gacttcgatg atgcctgctc catgagcttc aggcacgact cgcccattgc gccgaacaag
     7441 gcgtcgtaca gcgggcgcaa atagggatcg atcttttcct tgagatcgcc ggggagaaaa
     7501 cccagacgct cgcccgcttc cagcgccggg cgcgaaagga tgagtctttc cacccgccgt
     7561 tcgtgcagaa gatgcgcccc gaaggccgcc gccagatagg tcttgcccgt acccgcgggt
     7621 ccgatgccga aaaccagcgg atgggtgcgc atcaaatcga gatacgccgc ttgcgcgggc
//
LOCUS       DASWPU010000200         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_99678,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000200 DASWPU010000000
VERSION     DASWPU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99678"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /gene="lpdA"
                     /locus_tag="VGC27_04385"
     CDS             complement(<1..1247)
                     /gene="lpdA"
                     /locus_tag="VGC27_04385"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY0281846.1"
                     /translation="MEDQFDAIILGGGPGGYNAAIRLGQLGLATACIDKRGRFGGTCL
                     NVGCIPSKALLHASERYEEASRDFAKLGIKAEVSLDLNAMMAHKDKVVGELTKGVEFL
                     LKKSKSEAIVGEARIAAPGKVEVKSADGSLRTLKTKNIIVATGSDAMALPGVNIDEER
                     IVSSTGALALKQVPKRLLVVGGGYIGLELGSVWRRLGSEVLVVEFLDRITPGLDGETA
                     KQFQRILQKQGIKFRLSTKVVGVEETSGGLRVGLEAAAGGERQSAETDVLLVSIGRRP
                     YTDGLGLDKIGVALDGRGRVVTDAHFKTNVPGIYAIGDCREGVMLAHKAEDEAVACAE
                     RIAGRAGHVNYDAIPAVVYTAPEVASVGKSEEELKAAGIAYKVGKFPFSANARAKTIA
                     ATEGFAKVLADAKTDRVLGVHILG"
     gene            complement(1327..>2185)
                     /locus_tag="VGC27_04390"
     CDS             complement(1327..>2185)
                     /locus_tag="VGC27_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281847.1"
                     /translation="LILFALNLWWNGTHDWATFVFQFGRIEGGGFTLRYLIEFLGAQL
                     ILATPFILVLGVFGLAGATRDERQSVIAAMLWPAIAYFAYHSLHTRVQGNWPCFLYPL
                     LTVAAALAWQRTDWPSWRASVLRWSKRLAVPFAAVLLAAVYAQALFGVVPMGRKDPLA
                     RLLAVGFPEVAAKVEALRLTAQAGAVLTTDYASTGWFAFYGSGPVVQIGENYRWSGAP
                     VAAAPLLAGPVLYVTEIRRDRHDLIGTHFAEVTEIARIDRERQGIPIAHYVVYRASGL
                     KGAPVGRMP"
BASE COUNT          395 a          740 c          673 g          377 t
ORIGIN      
        1 cccagaatat gcacgccgag cacgcggtcg gtcttggcgt cggccagcac tttcgcaaac
       61 ccttccgtcg ctgcgatggt tttcgcccgg gcattggcgc tgaaggggaa cttgccgacc
      121 ttgtaggcga tgcccgccgc cttcaattct tcttcgctct tgccgacgct ggctacttcc
      181 ggcgcggtat agactaccgc cgggatcgca tcataattca catgcccggc acggccagcg
      241 atgcgttcgg cgcaggccac cgcctcgtcc tcggccttgt gggcgagcat caccccttcg
      301 cggcaatcgc cgatggcgta gatgccggga acattggttt tgaaatgcgc gtccgtgacg
      361 acgcgcccgc gcccatccag cgccacgccg atcttgtcca ggccgagacc gtccgtatac
      421 ggacgccgcc cgatcgagac cagcagcacg tcggtttccg cgctctgccg ctcgccgccc
      481 gccgccgctt ccaggccgac gcgcaatccg ccgctggtct cttccacgcc gacgaccttg
      541 gtcgaaagcc ggaacttgat gccctgcttc tgcaggatgc gctgaaactg cttggccgtc
      601 tcgccgtcga ggcccggggt gatgcggtcg aggaactcca ccaccagcac ctcgctgccg
      661 agccggcgcc agaccgagcc cagctcgagt ccgatatagc ccccgccgac caccagcagc
      721 cgcttgggca cttgtttaag cgccagcgcg ccggtggaac tgacgatccg ttcttcgtcg
      781 atattcacac ccggcagcgc catcgcatcc gagccggtgg cgacgatgat gttcttggtc
      841 ttaagcgtac gcagcgatcc gtcggcggat ttgacctcga ccttgccggg ggctgcgatc
      901 cgcgcctcgc cgacaatcgc ctcggacttg ctcttcttga gcagaaactc gacgcctttg
      961 gtaagctcgc ctaccacctt gtccttgtgc gccatcatcg cattcagatc gaggctgacc
     1021 tcggccttga tgccgagctt ggcgaagtcc ctacttgcct cttcgtagcg ctcgctggca
     1081 tgcagcagcg ccttggaggg gatgcagccg acattgaggc aggtcccgcc gaagcggccg
     1141 cgtttgtcga tacacgcggt cgcaagaccg agctggccca gccggatcgc ggcgttgtag
     1201 ccgccgggtc cgccgccaag gatgattgca tcgaattgat cttccatgcg cggggtcttt
     1261 cgtcgaggga gcgatgaagt gtaatgccgt ccggcgcgcc gccgcaattc gcgggcgagg
     1321 gtttcgctac ggcatacggc ccacgggagc gcccttgagg ccgctggcgc ggtaaacgac
     1381 ataatgggcg atgggtatgc cttgccgttc ccggtcgatg cgggcgattt ccgtgacctc
     1441 agcgaaatgc gtcccgatca gatcgtggcg gtcccgtctg atttcagtga cgtaaagcac
     1501 ggggccggcc agcagcggcg cggcggcaac cggtgcgccg gaccagcggt aattctcgcc
     1561 gatctgcacc acggggccag acccatagaa ggcgaaccag ccggtggagg cgtaatcggt
     1621 ggtcagcacg gcgccggcct gggcggtgag acgcagcgcc tccactttcg cggcaacctc
     1681 gggaaaaccc accgccagca gccgcgccaa cgggtccttg cgccccatcg gcacgacgcc
     1741 gaacagcgcc tgagcataaa cggcagcgag cagcacggca gcgaacggaa ccgccagtcg
     1801 cttcgaccag cgcagcacgg aggcgcgcca gcttggccaa tccgtgcgct gccaagccag
     1861 cgccgccgct actgtgagca gcggatagag aaagcaaggc caattgcctt gcaccctggt
     1921 atgcaaagag tggtaagcga aataggcgat cgcgggccat agcatcgcgg cgatcacgct
     1981 ctggcgctcg tcgcgcgtgg ctccggcgag cccaaacacg cccagcacca gaataaacgg
     2041 cgtagctaga atcagttgcg cgcccaggaa ctcgatcaga tagcgcagcg tgaagccgcc
     2101 gccttcgatg cgcccgaact ggaagacgaa agtcgcccag tcatgcgtcc cgttccacca
     2161 cagatttagt gcaaacagaa tcagc
//
LOCUS       DASWPU010000201         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_105851,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000201 DASWPU010000000
VERSION     DASWPU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105851"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VGC27_04395"
     CDS             complement(<1..454)
                     /locus_tag="VGC27_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEY0281848.1"
                     /translation="MTQLRKKAPAKVRPALKPAKGAGMHQVSPLAPKNFANLPPLAGV
                     RLASGEAGIRYKNRTDVLLAVFAPGTQVAGVFTKSKTASAPVDWCKANLKGGLARALV
                     VNSGNANAFTGKAGLEGVREIAAEAANVAGCRPQEVFMASTGVIGEPLP"
     gene            597..>2089
                     /locus_tag="VGC27_04400"
     CDS             597..>2089
                     /locus_tag="VGC27_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0281849.1"
                     /translation="MQGFGAIKRWFIELAQRFFGSANERKIKRFWSKVAEINALEPVF
                     VAKSDEELRAMTFSFKERLAKGETLEDILAEAFAAVREAAKRTLGQRHFDVQLVGGMV
                     LHYGNIAEMKTGEGKTLVATLAVYLNALAGEGVHVVTVNDYLAKRDAEWMGQIYRFLG
                     LTVGCIVHGLTDEERRENYACDVTYGTNNEFGFDYLRDNMKYSIPTMTQRGHSYVIVD
                     EVDSILIDEARTPLIISGPTEDLTELYRKVDKLMPGLVEGKAASKADPVGQPGKPGDY
                     ELDEKQRQVTLTDSGNEHMVQLLREAELLETGDLYDIENISILHHVNQALKAHKLFQK
                     DKDYIVKNDEVIIIDEFTGRMMQGRRFSEGLHQALEAKEGVTVQPENVTLASITFQNY
                     FRLYDKLAGMTGTALTEAAEFMDIYRLDVLEIPTNLPVKRVDADDEVYRTYDEKNEAI
                     IKLIEDCQTRGQPVLVGTTSIEKSEHLSAQLKKHKIAHNVLNARYHE"
BASE COUNT          447 a          646 c          640 g          356 t
ORIGIN      
        1 ccggcagcgg ctcgccgatc accccggtcg aggccatgaa gacctcctgc ggtcggcatc
       61 cggcgacatt ggccgcctcg gcggcgattt cgcgcacccc ctccagcccg gccttgccgg
      121 tgaaggcgtt ggcgttgccg ctgttcacca ccaaagcgcg ggccaaaccg cccttcagat
      181 ttgccttgca ccaatcgacc ggagcggaag ccgtcttgga cttggtgaat acacccgcca
      241 cctgcgtacc gggcgcaaac acggccagca gcacgtcggt gcgattcttg tagcggatgc
      301 ccgcttcgcc cgacgccaag cgtacgccgg ccaggggcgg cagattggca aaattcttgg
      361 gagccaaagg agaaacttga tgcattccgg cgcctttagc gggcttgagc gccgggcgta
      421 ccttggcggg cgccttctta cggagctgcg tcataatcgt cacccagaac cggcgggggg
      481 gtttggccgg aatcgtgtga tttgacaagg gggtgtgccg ttcttatctc tccgccgcgg
      541 caactggcgc cacattcgtg ccggccggcc ggtttctggc ccgaacagag gttttcatgc
      601 agggttttgg cgcgattaag cggtggttca ttgagctggc acagcgtttc ttcgggtccg
      661 ccaacgaacg gaagataaag cggttctggt ccaaggtggc ggaaatcaac gccctggagc
      721 ccgttttcgt cgccaaatcg gacgaagaac tgagggcgat gacgttcagc ttcaaagaac
      781 ggctggccaa gggcgagacc ctggaagaca tcctggcgga agcttttgcc gcagtccgcg
      841 aggcggccaa gcgcaccctc ggccagcggc atttcgatgt ccagctggtc ggcggcatgg
      901 tcctgcacta cggcaatatc gccgagatga agaccggcga aggcaaaacc ctggttgcca
      961 ccctggcggt ctatctgaac gcgctggccg gcgaaggtgt ccatgtcgtg accgtcaacg
     1021 actatctggc caagcgcgac gccgaatgga tgggccagat ctacaggttc ctcggcctga
     1081 ccgtcggctg catcgtccac ggcctgaccg acgaggagcg ccgcgagaac tatgcctgcg
     1141 acgtgaccta cggcaccaac aacgaattcg gcttcgatta tctgcgcgac aacatgaagt
     1201 attccatccc gacgatgacc cagcgcgggc attcctacgt catcgtcgac gaagtggact
     1261 ccatcctgat cgacgaggcg cgcacgccgc tgatcatatc gggtccgacc gaagacctga
     1321 ccgaactgta ccgcaaagtc gacaagctga tgccgggcct ggtcgagggc aaggcggcgt
     1381 caaaagccga tccggtgggg cagccgggca agccgggaga ctacgaactc gacgagaaac
     1441 agcgtcaggt gacgctgacc gattccggca acgagcacat ggtccaattg ctgcgcgagg
     1501 cggagctgct ggaaaccggc gatctctacg atatcgagaa catctcgatc ctgcatcacg
     1561 tcaatcaggc gctgaaagcg cacaagctgt tccaaaagga caaggactac atcgtcaaga
     1621 acgacgaggt catcatcatc gacgagttca ccggccgcat gatgcaggga cggcgcttct
     1681 ccgaggggct gcaccaggcc ctggaggcga aggaaggcgt cacggtgcag ccggagaacg
     1741 tgacgctcgc ctcgatcacc ttccagaact atttccgcct ctacgacaag cttgccggca
     1801 tgaccggcac cgcgctgacg gaagcggccg agttcatgga catctacagg ctggatgtcc
     1861 tcgaaattcc gaccaatctg cccgtcaagc gcgtcgatgc cgatgacgag gtctatcgca
     1921 cctatgacga gaagaacgag gccatcatca agctgatcga ggactgccag acgcggggcc
     1981 agccggtcct cgtcggcacg acatcgatcg aaaagtcgga acacctctcc gcccagctca
     2041 agaagcacaa gatcgcgcac aacgtgctga acgcgcgcta tcacgagca
//
LOCUS       DASWPU010000202         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_111993,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000202 DASWPU010000000
VERSION     DASWPU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111993"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2079
                     /gene="mfd"
                     /locus_tag="VGC27_04405"
     CDS             <1..>2079
                     /gene="mfd"
                     /locus_tag="VGC27_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY0281850.1"
                     /translation="LHAAGKRVIVAAWTEGSAERMGGVLADHGLSVLRRVADWPDAQK
                     MTARAVGLAVLGIEHGFETPDFALVSEQDILGDRMVHSHTRARRAQNFLSEASSLSPG
                     DLVTHIEHGVGRYVGLQTIEVTGAPHDCLELQYDGGKLFLPVENIELLTRYGAEEGAV
                     LDRLGGAGWQQRKARAKERVREIASELIRIAAARELKSLPSADPPQGLYDEFCARFPY
                     QETEDQEKAIADAIEDLARGRPMDRLVCGDVGLGKTEVALRTAFVAAMAGSQVAVVVP
                     TTLLARQHTKLFNERFAGFPLKVRQLSRFVPAKEARETKAALASGDVDIVIGTHALLA
                     KSVTFQNLGLVIVDEEQHFGVVHKERLKALKSDVHVLTLTATPIPRTLQLALSGVRDL
                     SLITTPPIDRLAVRTFVTPFDPLVLREALLREHYRGGQSFYVVPRISDLGEAQAFLKA
                     TVPEVKSAAAHGQMAPNALEDVMTSFYDGKVDVLVSTNIVESGLDIPTANTLIVHRAD
                     QFGLSQLYQLRGRIGRSKQRAYAYLTTPPDRKPTESAERRLEVLQSLDQLGAGFTVAS
                     HDMDIRGAGNLLGEEQSGHVREVGIELYQEMLEEAVSQMRSGEGEAELADQWSPQINI
                     GASVLIPETFVPDLNVRMALYRRLAGIETRDDIERFAAELIDRFGPLPEEVRHLFEIV
                     AIKQLCRAASV"
BASE COUNT          342 a          690 c          700 g          347 t
ORIGIN      
        1 ctgcacgcgg cgggcaagcg ggtgatcgtt gccgcctgga cggagggttc ggccgagcgc
       61 atgggcgggg tgcttgccga tcacgggctg tccgtcctcc gccgcgtcgc cgattggccg
      121 gatgcgcaaa agatgaccgc gcgcgccgtg gggctggccg tgctcggcat cgagcatggt
      181 ttcgagacgc ccgatttcgc cctggtgagc gagcaggaca ttctcggcga ccgcatggtg
      241 cattcgcata cgcgggcgcg ccgggcgcag aattttctct ccgaggcttc cagcctttcg
      301 ccgggcgatc tggtcaccca catcgaacac ggcgtcggac gctatgtcgg tttgcagacc
      361 atcgaggtga ccggcgcgcc gcacgattgc ctggaactgc aatatgacgg cggcaagctg
      421 ttcctgccgg tcgagaacat cgaacttctc acccgctacg gcgcggagga gggcgcggtc
      481 ctcgaccgcc tcggcggcgc gggctggcag cagcgcaagg cgcgcgccaa ggagcgggtg
      541 cgcgaaatcg cctccgaact gatccgcatc gccgccgcgc gcgaactcaa gtcgctgccc
      601 tccgccgatc cgccgcaggg cctttacgac gaattctgcg cgcgtttccc ctatcaggag
      661 accgaggacc aggagaaagc catcgccgac gccatcgaag atctggcgag aggccggccg
      721 atggaccggc tggtctgcgg cgatgtcggc ttaggcaaga cggaagtggc gctgcgcacg
      781 gccttcgtgg cggcgatggc gggcagccag gtggccgtcg tggtgccgac cacgcttctg
      841 gcgcgccagc acaccaagct cttcaacgag cgcttcgccg gctttccgtt gaaggttcgc
      901 caattgtcgc gcttcgtgcc cgccaaggag gcgcgcgaga ccaaggccgc gctggcctcc
      961 ggcgacgtgg acatcgtcat cggcacccat gcgctgctcg ccaagagcgt gacgtttcaa
     1021 aatctcgggc tggtgatcgt ggacgaggag cagcatttcg gcgtcgtcca caaggaacgg
     1081 ctgaaggcgc tgaaatccga cgtgcatgtg ctgacgctga ccgccacgcc gattccgcgc
     1141 acgctgcaac tggcgctgtc gggggtgcgc gacctgtcgc tgatcaccac gccgccgatc
     1201 gaccgtttgg cggtgcgcac cttcgtgacg ccgttcgatc cgctggtcct gcgcgaagcg
     1261 ctgctgcgcg agcattatcg cggcgggcag agtttctacg tcgtgccgcg catctccgac
     1321 ctgggcgagg cgcaggcgtt tctcaaggcg acggtgccgg aggtgaaatc ggccgccgcc
     1381 cacggccaga tggcgccgaa cgccctggaa gacgtgatga cctcgttcta cgacggcaag
     1441 gtggacgtgc tggtttccac caacatcgtc gagagcggcc tcgacattcc caccgcgaac
     1501 actctcattg ttcaccgcgc cgaccagttc gggctgtcgc agctttacca gttgcgcggc
     1561 cgcatcggcc gctccaagca gcgcgcctac gcctatctga cgacgccgcc cgaccgcaag
     1621 ccgaccgaat ccgccgagcg ccggcttgag gtgctgcaat cgctcgacca gttgggcgcg
     1681 ggcttcaccg tcgccagcca cgacatggac atccgcggcg cgggcaatct gttgggcgag
     1741 gagcaatccg gccatgtgcg cgaggtcggc atcgagttgt accaggagat gctggaagaa
     1801 gccgtctcgc agatgcgcag cggcgaaggc gaggccgagc ttgccgacca atggtcgccg
     1861 cagatcaaca tcggcgcctc cgtgctcatt cccgaaacct ttgtgcccga tctcaacgtc
     1921 cgcatggcgc tgtaccgcag gctggcgggg atcgagaccc gcgacgacat cgagcgcttc
     1981 gccgccgagc tgatcgaccg cttcggtccg ctgccggaag aggtgcgcca tctgttcgag
     2041 atcgtggcga tcaagcagct ttgccgcgcg gcctcggtg
//
LOCUS       DASWPU010000203         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_118053,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000203 DASWPU010000000
VERSION     DASWPU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118053"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            649..765
                     /locus_tag="VGC27_04410"
                     /pseudo
     CDS             649..765
                     /locus_tag="VGC27_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611246.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            914..1039
                     /locus_tag="VGC27_04415"
     CDS             914..1039
                     /locus_tag="VGC27_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281851.1"
                     /translation="MEIVLEGGRRIIVGKDVDAAALLRIVDVLERRPVSRSYGEG"
     gene            1039..1386
                     /gene="tnpB"
                     /locus_tag="VGC27_04420"
     CDS             1039..1386
                     /gene="tnpB"
                     /locus_tag="VGC27_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY0281852.1"
                     /translation="MIPVPAGVHVWIATGHTDMRKGFDGLAVRVQEVLKRDPHSGHLF
                     VFRGKRGDLVKLLWHDGQGMCLFAKRLERGRFIWPSAAGEAVTISAAQLGYLLEGIDW
                     RAPQRTYRPELAG"
     gene            1473..2999
                     /locus_tag="VGC27_04425"
     CDS             1473..2999
                     /locus_tag="VGC27_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEY0281853.1"
                     /translation="MKLDLSALPRDVEALQNLVREQAALLQSKDLEIERLTARLDKLR
                     RMQFGRSSEKITNEIEQLELLLDDLHENQGDRAERPAVVPETAQKPARRPLPAHLPRE
                     EVAHAAACICPKCGGELRRLGEDVTEVLEYVPASFKVIRHVRPKFSCRRCEAITQAPM
                     PSLPIERGRPGPGLLAHVLVSKYADHLPLYRQSEIYAREGIELERSTLADWVGGSAAL
                     LAPLVEALRKDVMASDVLHGDDTPVPVLAPGTGKTRTGRLWTYVRDERPHAGERPAAA
                     VFFYSPDRKGERPQNHLKSFKGMLHADGYAGFNVLFETGKIAEAACWAHVRRKFYDVH
                     AANGSPLAKEALDRIGALYRIEDEVRGGPPDERRRTRQERTKPLLADLHDWLAATKPK
                     LSPKSDLAAAIRYTLARWTARTRYADDGRLEIDNNAAERAIRPLALGRKNYLFAGSDK
                     GGERAAAIYTLIGTTRLNGLDPEAYLRDVLARIADHPINRIGELLPWNWKAASAKLAA
                     "
     gene            2996..3577
                     /locus_tag="VGC27_04430"
     CDS             2996..3577
                     /locus_tag="VGC27_04430"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HEY0281854.1"
                     /translation="MSDVVRLKITLVDTEPAIWRRVEVPSAITLKDLHAVIQAAMGWE
                     NSHLYQFHVGRQTINGPGFVDVGFSGQHNITAARVGLNELVESKVKRFDYLYDMGDSW
                     EHELRVEKVLPAEPATRYPRFIGGAGACPPEDVGGLPGFYNLLEALEDPKHPNHEDML
                     DWCGGRFDPNALDEASIRKRLSRLGPRKKRKPA"
BASE COUNT          715 a         1184 c         1239 g          702 t
ORIGIN      
        1 ggtcatcgcc tacagttacg atgctgcagg aaacagaaca tctagttcca ttacaggttc
       61 cggtaccacg ggtacaattt gggggcagtt cgcttggggg gctgcctcct gggcggcgtt
      121 tggcggcgga ggctcggcgc cgattggatc cctctccgct aatccgagca cagtcgcaca
      181 aggtagttcg tcaacgttga cgtggtcgag cagcaacgcg acaagcgtat ctatcgacaa
      241 tggtgttggc attgttgcgc cagtgcctag cagctcggtc aaggtctcgc cgatttcgac
      301 gacgacctat accttgaccc tcacagggcc ggagggaatg accacccaga cggcgacggt
      361 gacggtaaat taggcaccga ccggcgtctt ctcggcgaat ccgacgacga ttacggccgg
      421 tcaatcgacc acgctgagtt ggacgagttc caatgcgacc agcgtgaccc tcaatggcaa
      481 cgccgttgcg gtcagcgaca gccagagctt taggtaagcg tccggtgatg accgccttgc
      541 gctgtggtta ggttgggaca cgatgggtct tattggatcg ataggaccta tatcgtgcct
      601 atgtctggac atggtgcagt gacggaggtt gaggtcattg cctcgacgcg gcggcggtgg
      661 tcggcagcgg agcggcggac gatcctcgcc gaggctgatg agtcgggtgt gagcgtgtcg
      721 gaggttgcgc gccgacacgg gttgtcaccg agcctcctgt tccgctgacg acgtgaactg
      781 gggaagtcga agaagagagc gacgccggag ccggcgtttg ttccgcttgc gctgcccgcg
      841 cccgcttgcg ctgcccgcgc cggcttcatc accggacgac cgatctccag tcccgggtct
      901 gggcgtggac aggatggaga tcgttcttga aggcgggcgt cggatcatcg tcggcaagga
      961 cgtggatgcg gcggcgctgc tgcgcatcgt ggatgtgctg gaacggcgac ccgtcagccg
     1021 aagctatggc gaaggctgat gattccggtt ccggctggtg tgcatgtctg gatcgcgacc
     1081 ggtcacaccg acatgcgcaa gggctttgac ggcctggcgg tgcgtgtcca ggaggtgctg
     1141 aagcgcgatc cgcactccgg acatctcttc gtgttccgcg gcaagcgcgg agatctggtg
     1201 aagctgctgt ggcacgacgg ccagggcatg tgcctgttcg ccaagcggtt ggaacgcggt
     1261 cggttcatct ggccgagcgc tgcgggcgag gcggtgacga tctcggcagc gcagctcggc
     1321 tatctactgg aagggatcga ctggcgagca ccgcagagaa cgtatcggcc cgagcttgca
     1381 ggttgatcgg atcgtacttc aacagcgcgg ttgattgact cgcgacgttg catcctctgt
     1441 gaaccggaat actccgcatt acgctcgcgg gcatgaagct cgatttgtct gccctgccta
     1501 gggatgtcga agcgctgcaa aacctggtgc gcgagcaggc ggcgctgctt caatcgaagg
     1561 atctggagat cgaacggctg acagcgcggc tcgacaagct gaggcggatg cagtttggcc
     1621 gctcctcgga gaagatcacc aacgagatcg agcagctcga gcttctgctg gatgacctgc
     1681 acgagaacca aggtgatcgc gccgaacgcc cggcggtggt gcccgagaca gcgcagaagc
     1741 cggcccgtcg tcccttgccc gcgcatctgc cgcgtgaaga ggttgcgcat gccgcggctt
     1801 gcatctgtcc caagtgcggc ggcgagctgc gccggttggg cgaggacgtc accgaggtgc
     1861 tggagtacgt gccggcctcg ttcaaggtga tccggcatgt gcggcccaag ttctcctgcc
     1921 ggcggtgcga ggccatcacc caggcgccga tgccatcgct tcccatcgag cgcggacggc
     1981 ccggacccgg tctcctggcc catgtgctgg tgtcgaagta cgccgatcac ctgccgctct
     2041 atcgccagag cgagatttac gcgcgcgaag gcatcgagct ggagcgctcc acactggccg
     2101 attgggtggg cggctcggcg gcgcttttgg cgccgctggt ggaagccctg cgcaaggacg
     2161 tgatggcctc ggatgtcctg cacggcgacg acacgccagt gccggtgctg gcgccgggaa
     2221 ccggcaagac cagaacgggt cggctttgga cctatgtccg tgatgagcgg ccccatgccg
     2281 gcgaacggcc tgcggcagcc gtgttcttct actccccgga tcgcaagggc gaacgccctc
     2341 agaatcatct gaagagcttc aaaggcatgc ttcacgccga tggctatgcc ggcttcaacg
     2401 tcctgttcga gaccggcaag attgccgagg cggcctgctg ggcgcacgtc cggcgtaagt
     2461 tctacgacgt ccatgccgcg aacggctcac cgctcgctaa ggaggcgctc gaccgcatcg
     2521 gggctctcta ccggatagaa gacgaagtcc gcggcgggcc accggacgaa cgacgacgaa
     2581 cgcgtcagga gcgaacaaag ccgctgctgg cggacctgca tgattggctc gccgcgacca
     2641 agccgaagct gtcaccaaaa tccgatcttg ccgccgcgat ccgctacacc ctggcgcgct
     2701 ggaccgcgcg cacgcgctac gccgacgacg gcaggctgga gatcgacaac aacgccgccg
     2761 agcgtgccat ccgccccctg gccttgggcc gcaagaacta cctcttcgcc ggttccgaca
     2821 agggcggaga gcgcgctgca gcaatctaca cgttgatcgg caccaccagg ctcaatggcc
     2881 tcgatccgga agcctatctg cgcgacgtgc tcgcccgcat cgccgatcat cccatcaaca
     2941 ggattggcga gttgctgccg tggaactgga aagccgcctc tgccaaactg gcggcatgag
     3001 cgatgtcgtc cgcctgaaga tcacgcttgt cgataccgag cccgccatct ggcgcagggt
     3061 cgaggttccg tccgccatca cactcaagga cctgcacgcc gtcatccagg ccgcgatggg
     3121 ctgggagaac tcccatctct atcagttcca tgtcgggcgc cagaccatca acggccccgg
     3181 cttcgtcgat gtcggcttct ccggccagca caacatcaca gcggcacgcg tcgggctcaa
     3241 cgagcttgtc gaaagcaagg tcaagcgctt cgattatctc tacgacatgg gagacagctg
     3301 ggaacatgag ctgcgcgtcg agaaggtgct gcccgccgag ccggcgacga gatatccccg
     3361 cttcatcggc ggcgccggtg cctgtccgcc cgaagatgtc ggcggcttgc ctgggttcta
     3421 caacttgctc gaagctctcg aagaccccaa gcatccaaac cacgaagata tgctcgactg
     3481 gtgtggtggt cgcttcgatc ccaacgcgct cgacgaagca agtatccgca aacgcctttc
     3541 ccgcctcggt ccccgcaaaa agcgcaaacc cgcctgagcc cccgcggcct tcaccggacg
     3601 cttactttgg ggtgaccttt cggcaaacgg agacggttcg gaagagcgcg tgagaattgc
     3661 ccgctcaggc gttcttcacc cccatttcgt cgcactcatc tctacgaaat gccccgcata
     3721 cggccggttc atggccgtgc actcgtacgg gtgagacgga gcaggaatcg ctggcgcagg
     3781 aaggagtctg ttaaaactct gctccaaagt ctgcaatatc ttagagcatg ttgaattcat
//
LOCUS       DASWPU010000204         5703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_87040,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000204 DASWPU010000000
VERSION     DASWPU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87040"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VGC27_04435"
     CDS             <1..400
                     /locus_tag="VGC27_04435"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEY0281855.1"
                     /translation="YFNHEGIIDTIEGADADLVCADISNGVKYYEADIDPDGDPVAAI
                     ASHYLAQNTSARQLDTDIRMRHLLDLVEAYHVDCVIYYVLKFCDTNLHDYPYIKQALR
                     ARNIPVLLIEGEHNASNIASARTRIQTFLE"
     gene            410..1633
                     /locus_tag="VGC27_04440"
     CDS             410..1633
                     /locus_tag="VGC27_04440"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEY0281856.1"
                     /translation="MQTETTARQRNNQYRNLLALMANSGPERGSNKSALQQTISMRSI
                     MNCHRLAEAYKEDTRVAYISEQFPSEIVFASGALPWNIESMAIMLAQSIDVNRIFQLT
                     QEYELSRDICSFLRGPHGMMLADCYPTPDVVLTNDQPCEGLAKMMTLAAKHYRAPVLS
                     LNTPEFIDGDSIDYLAAQIERVMRQMDELWQWKRDPEALRSAVRLSNEAREYYCKTAQ
                     LFERRALPGVSREMQEIFGMNYFGSRQNVLVCKALYEEALKLADGNEPAGAKRVLWIG
                     QVPEESHELLEHMRRVVDIVYWAPLWASNLRFLDEDKPLHSMAERAMFYHWSAERMRA
                     DTAAICEKYNIEGLLIVNVWGCRNVAGISATLRDLATAMKLRHLTINIDLVDRNNYSF
                     SHVKHRVDAFLELLQ"
     gene            1608..2438
                     /locus_tag="VGC27_04445"
     CDS             1608..2438
                     /locus_tag="VGC27_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEY0281857.1"
                     /translation="MRSSNCYSEGRRKVVITAGIDVGSLSTKAALMDECGKVIAYDVI
                     LTGGSNRDAAEATYDNALAKTGLARKDVAFVVTTGYGRENVGFADKHVSEITCHAAGM
                     HLTNPRIRTILEIGGQDSKAIRIDENGNVEHFAMNDKCAAGCGRFLEVTAKAMGLELK
                     ELGEVSARSQNCVRISSTCTVFAETEVVSLVAVGTPIPDIVRGVHSSIATRASLLLRT
                     VTINGPLGMSGGVALNSGVVTALGEVLKTEVYVADNPQVRGAIGAAFVGQKQVALRAA
                     "
     gene            2457..3884
                     /locus_tag="VGC27_04450"
     CDS             2457..3884
                     /locus_tag="VGC27_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281858.1"
                     /translation="MSLSAAETMRYQAIGRHNISRVPEWNRLASDLRAVVEVVSQVLP
                     FRANRYVLSNLIDWSRVPDDPIFQLIFPQREMLTREQYREVEAPLRKGDKPALNRVVD
                     RIRLDLNPHPAGQLTHNVPLLDGRPLWGVQHKYRETVLFFPFQSQTCHAYCTYCFRWA
                     QFVGMKGLKFASRDTKDVVAYLKVHPEVTDVLITGGDPMIMRTAMLRRYIEPFLSPEL
                     AHVQNIRIGTKAVASWPHRFVSDADADDCLRLFEEVVAKERHLAIMGHYSHPAELETA
                     IAREAIRRIRSTGAEIRMQAPLVKHVNDDPKAWSTLWQTGVRLGLVPYYMFVERDTGA
                     RKYFEVPLVRALDIYRQAVAAVSGLSHTVQGPSMSALPGKVRIAGTTTLRSIIGERAA
                     DALRQISGRLAEINPDRPVLVCEFIQARDPALIRTLFFAEYDAQAAWLDQLRPAFGQR
                     NFLFEGGYGHAADAPLRSVTECLLN"
     gene            complement(3926..4621)
                     /locus_tag="VGC27_04455"
     CDS             complement(3926..4621)
                     /locus_tag="VGC27_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY0281859.1"
                     /translation="MHDGDTVEIVLLSADITGPALSEIGAVLTTAETDRAHHFHFEAD
                     RRRWIVGRASLRYLLSRRLGIPPQSLQFGCGTHGKPFLPASRLHFNLSHSGNVVAIAL
                     AASDVGIDVEERHDIPDMHQVASRFFSPLELAGINAATDPIGVFFRIWTMKEAVVKAL
                     GGGLSLPLEHFTVWFADSKTLPTGVLAASPIIAGFSAAGLYVREGYYGAVARRGQGWR
                     LAVHWENVLDLVP"
     gene            complement(5233..5308)
                     /locus_tag="VGC27_04460"
     tRNA            complement(5233..5308)
                     /locus_tag="VGC27_04460"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5273..5275),aa:Phe,seq:gaa)
BASE COUNT         1208 a         1744 c         1666 g         1085 t
ORIGIN      
        1 ctacttcaac cacgagggga tcatcgacac gatcgaaggc gccgatgccg accttgtttg
       61 tgccgacatc tcgaacggcg ttaagtatta cgaggccgac atcgacccgg atggcgatcc
      121 cgtcgccgcc atagccagcc attatctcgc gcagaacacc agcgcccgcc aactcgatac
      181 ggacatcagg atgcgacacc tcctggacct ggtcgaggcg tatcatgtcg attgcgttat
      241 ctactacgtg ctcaagttct gcgacacgaa cctgcacgat tatccctaca tcaagcaggc
      301 cctgcgcgcc cggaatatcc cagttttgct gatcgaaggc gaacacaacg cgtcgaatat
      361 cgccagcgcg cggacgcgga tccagacttt tctggagtaa ggaggctacg tgcagaccga
      421 gacgacagcg cggcaacgca acaaccagta ccggaacttg ctggcgctca tggccaacag
      481 cggaccggaa cgtggcagca acaagagcgc gctgcagcag accataagca tgcgctccat
      541 catgaactgc caccggctcg ccgaggccta caaagaagac acaagggtcg cctacatctc
      601 ggagcaattc ccgagcgaga tcgtgttcgc cagcggagcg ttaccctgga atatcgaatc
      661 catggcgatc atgctggccc aatccatcga cgtgaacagg atattccagc tcacgcaaga
      721 atacgaactg tccagggaca tctgctcctt cctgcgcgga ccgcacggca tgatgctcgc
      781 ggactgctat cccacgcccg acgttgtgct gaccaacgat cagccctgcg agggcttggc
      841 gaagatgatg accttggcgg caaagcacta tcgcgcgccc gttctctccc tcaacacgcc
      901 ggaattcatc gatggcgatt ccatcgacta tctcgccgcc cagatcgaac gcgtgatgcg
      961 ccagatggac gagctctggc agtggaaacg cgatccggag gcgctgcgct ccgccgtcag
     1021 gctctcgaac gaggcccggg aatactattg caagacggcc caactcttcg aacggcgcgc
     1081 cttgcccggg gtatcccgcg agatgcaaga gattttcggg atgaactatt tcggcagcag
     1141 gcaaaatgtt ttggtctgca aggcgctcta tgaagaggcg ctgaaactgg ccgatggtaa
     1201 cgagccggcc ggcgcgaagc gtgtgttgtg gatcggccag gttcccgagg agtcccacga
     1261 gttgctcgaa catatgcggc gcgttgtcga cattgtgtac tgggcgccgc tctgggcgtc
     1321 caacctgcgt tttctcgacg aggacaaacc gcttcacagc atggcggagc gcgcgatgtt
     1381 ctatcattgg agcgcggagc ggatgcgggc cgatacggcg gccatctgtg aaaaatacaa
     1441 cattgagggt cttctgatcg tcaacgtttg gggatgccga aacgtggcgg gaatcagcgc
     1501 cacgctgcgg gatctcgcga cggccatgaa gctcaggcac ctgaccatca acatcgacct
     1561 ggtggacagg aacaattact cgttcagtca tgtgaagcac cgggtcgatg cgttcctcga
     1621 actgttacag tgaagggaga aggaaagtgg tgatcacagc aggtatagac gtgggttcat
     1681 tgtcgaccaa agccgcgctc atggacgagt gcggcaaggt catcgcctat gacgttatat
     1741 tgaccggcgg cagcaacagg gacgcggcgg aagcaaccta tgacaacgcg ctggccaaaa
     1801 ccggacttgc gcgaaaggac gtcgcatttg ttgtcaccac cggttacggc agggagaatg
     1861 tcggctttgc cgacaagcat gtctccgaga tcacttgcca cgctgccggt atgcatttga
     1921 ccaatccgcg gatccgcaca atcctcgaaa tcggcggcca ggacagcaaa gccattcgca
     1981 tcgacgagaa tggaaacgtc gagcacttcg caatgaacga caaatgcgcg gccgggtgcg
     2041 gcagattcct ggaggtcacg gccaaggcga tggggctgga actgaaggag ctcggcgagg
     2101 tctcggcgcg gtcccaaaac tgcgtccgca tttccagcac ctgcacggtg tttgccgaga
     2161 ccgaagttgt gtcgctcgtt gcggtgggaa cgccgattcc cgacattgtc aggggcgtcc
     2221 acagctccat cgccacgcgc gcaagcctgc tgctgcggac cgtgaccatc aacgggccgc
     2281 tgggcatgag cggaggggtc gctctcaatt ccggtgtcgt cacggccctc ggcgaagtgc
     2341 tgaaaacgga ggtctatgtc gccgacaatc cgcaagtcag gggtgccatc ggcgcagcct
     2401 tcgtcggaca gaagcaggtc gcattgcgcg cggcttgaga gcgggagcgg atcacaatga
     2461 gcctgagtgc ggcagagacc atgcgctacc aggccatcgg gcgccacaac atctcgagag
     2521 tgcccgaatg gaatcggctg gcgtccgatc tgcgtgccgt ggtggaggtg gtttcgcagg
     2581 ttcttccctt ccgggcgaac cgctatgtcc tgtcgaacct catcgactgg tcgcgggtgc
     2641 ccgacgaccc gatcttccaa ctcattttcc cgcaacgcga gatgttgacg cgcgagcagt
     2701 accgcgaggt ggaggctccc ctccgcaagg gcgacaagcc cgctctcaac cgtgtggtcg
     2761 atagaattcg gctcgatctc aacccacatc ccgccggaca gctcacgcac aacgttcctc
     2821 tgctcgacgg ccgcccttta tggggggtgc agcacaagta ccgggagacc gtactgttct
     2881 ttcccttcca gtcgcagacc tgccacgcat actgcaccta ttgcttccgt tgggcgcaat
     2941 tcgtcgggat gaagggcctg aaattcgcca gccgggacac gaaggacgtc gttgcgtacc
     3001 taaaagttca tcccgaggtg accgacgtcc tcatcaccgg cggcgatccc atgatcatgc
     3061 gaacggccat gctgcgccgc tatatcgagc ccttcctgag ccccgaattg gcgcatgtgc
     3121 aaaatatccg gatcggcaca aaggctgtcg cctcatggcc ccaccgcttt gtctccgacg
     3181 cggacgccga cgattgcctc agattgttcg aagaggtcgt cgccaaggag cggcacctgg
     3241 cgatcatggg gcactactct catcccgccg aacttgagac cgcgattgcg cgcgaagcca
     3301 tccgccggat tcgcagcacg ggcgccgaaa tccgcatgca ggcgcccctg gtcaagcacg
     3361 tcaatgacga tccaaaggcc tggtccacgc tgtggcaaac gggtgtgagg ctggggctgg
     3421 ttccctacta tatgtttgtc gagcgggaca ccggcgcaag gaagtacttc gaggtaccgc
     3481 tggtgcgtgc ccttgatata taccggcagg cggtcgccgc cgtgtcgggt ctttcccaca
     3541 ctgtgcaagg accgtccatg tccgcactgc ccggtaaggt gcggattgcc ggcacgacca
     3601 cgctgcgcag catcatcggc gagagagccg cagacgcgct gcgccaaatt tccggtagac
     3661 tggcggagat caatccggac cggcccgttt tggtttgcga gttcatccag gcgcgcgacc
     3721 ccgcactgat tcgcacactc ttcttcgccg aatatgatgc acaagccgcg tggctggatc
     3781 agctgcggcc ggcgttcggc cagcggaatt tcctgttcga aggcggttac ggccacgctg
     3841 cggatgcgcc gcttcgctcc gttaccgagt gcctgctcaa ctgacctgcg ccccgctgca
     3901 atcggcagga gcgcaggtcg gcggctcagg ggaccagatc gagcacattc tcccagtgga
     3961 ctgccaagcg ccagccctgc ccgcgcctgg cgactgcacc ataatagccc tcgcgaacat
     4021 acaaacctgc ggcggagaaa ccggcgatga tcggtgacgc ggcaagaacc ccggtgggca
     4081 gcgttttcga gtccgcaaac caaaccgtga agtgctctag cggaagggac aggccgccac
     4141 ccaacgcctt taccacggcc tctttcatcg tccagatacg aaagaagacg ccaatcgggt
     4201 ccgtcgccgc attaatgcct gccaattcga gcggcgaaaa gaagcgggat gcgacctggt
     4261 gcatgtccgg tatgtcgtgg cgttcctcga catcgatccc gacatcgctg gccgcaagcg
     4321 cgatcgctac gacgttgccc gaatggctca gattgaagtg aaggcgggac gccggcagga
     4381 acggcttgcc gtgtgttccg catccgaact gcagactttg gggggggatg ccgagccggc
     4441 gcgacagcaa ataccgcaac gatgcgcggc cgacgatcca tcgccgccgg tcggcctcga
     4501 aatgaaaatg atgggcgcga tccgtctccg cagtcgtcag cactgcaccg atctccgaca
     4561 gagcgggtcc agtaatgtcg gctgatagaa ggacaatctc gacggtatcg ccgtcgtgca
     4621 tcacacgccg tcctagacaa ggcgaagacc attatatcgt cccgggctgg cccggaggat
     4681 acgtttctgc ttgccccgga atttcagtcc aacctcgatg tcgcaggcct gctcacctgc
     4741 cgaatgacga tatcgaggct agggacctat taacgtgctt gcggtcagag gttgatttat
     4801 tggctgcgca tccacacctg ctacgaccga cgcgcccaca ccttcttctc ggccatcggc
     4861 acagccgcca cctttacggc aatcaataaa ccccgaacct agaccggacg cataccggaa
     4921 gtactcgcca atctgctcgg cgtgcctttc gatacaagct gcaccgcccc ctcgcacccc
     4981 ctattccgcg ccactgagaa gtcaaaacac ccgaccgatg ctcacaacat gcactctcat
     5041 cggtgtgaac acccgttcgc gcaaaatgag aacaaaacat gagacatcat cccgcaacta
     5101 tcctgcttga gactctgata cccttcgtaa tccattgatt ttgcttggaa cggttcgtca
     5161 ctgtttggcg tgccaaaaca tgtgccaaat gcgtctgcgt ccttctataa caaattgatt
     5221 tcattgagaa aatggtgccc aggggcggaa ttgaaccacc gacacgcgga ttttcagtcc
     5281 gctgctctac caactgagct acctgggcat ctccggcacg gaagaccggg ggacgggctt
     5341 tttaggcaag cggggggccg ctgtccagcg gcaagctccg gctttggtaa gcacttcgcc
     5401 gtggccgggt ctccccagag tatttcaccg tttcacggaa acgatgaaat actctagatt
     5461 ctaggtttgt cgcgttttcg gacggaaaac cgcccggcgc gccttgcgcg cctattggtt
     5521 ccatagcgcg ctttcgtgcg ggcacttttc ctgaaaacgc tctagcaggc tgttgaagaa
     5581 gtttttcagg gttgggcgat gaagtcgatt tcgtcgccgt agtggacgca gatttgaccc
     5641 tggagggagc cgtcgggctg aagttcggcc cagccatcgc cgctgacctc gtccatttcg
     5701 tca
//
LOCUS       DASWPU010000205         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_99747,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000205 DASWPU010000000
VERSION     DASWPU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99747"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VGC27_04465"
     CDS             <1..198
                     /locus_tag="VGC27_04465"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0281860.1"
                     /translation="NDRGEGCFLNIALGVVGALVGGGIFALLGEPVFFRFSLWSMFVA
                     VLGAVIVLYLWHAVTGRRRLR"
     gene            282..695
                     /locus_tag="VGC27_04470"
     CDS             282..695
                     /locus_tag="VGC27_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281861.1"
                     /translation="MFRTAVTAAFLAAFVGQANAAAITDWNQIAKVFDTIPSAAGISV
                     HKRMDKCGVKIVKDKSWMETRAVDWPEYGARAGETVLLITIKIPSAAGQPPSLQQGTK
                     AIWLISRGKPMSVNAWAQQFQSRTVPMGYDAWMNC"
     gene            765..965
                     /locus_tag="VGC27_04475"
     CDS             765..965
                     /locus_tag="VGC27_04475"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEY0281862.1"
                     /translation="MAESISSREIMCLLEKAGWQEVRTTGSHHHFRHAKRLGTVTVPH
                     PVKDMPPGTVKSIERQSGVKLR"
     gene            969..1418
                     /locus_tag="VGC27_04480"
     CDS             969..1418
                     /locus_tag="VGC27_04480"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY0281863.1"
                     /translation="MQRFYTAIIEKAKDGYGVFFPDLPGCTSFGRSVEEVASNAYTAA
                     QAHVALSREYGDAIAEPRAMDAIPAERGVKEKARLLIPVEMADEPVRVNISLPSSALE
                     ALDRTAKELSLTRSGAIAYLALDRDSRTDQVAGRAKMKPRKRSVTSV"
     gene            1528..>2040
                     /locus_tag="VGC27_04485"
     CDS             1528..>2040
                     /locus_tag="VGC27_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0281864.1"
                     /translation="MADDRSRQKLMEFLEYLANKGLMAENTVAARKSAVSKILGILSE
                     SEAVDVTQLDLDQVMVRFQNLEGKNYTPGSMNTYLSRLRSAIDDFRTYLQNPLAFKPS
                     VQAREKRAKPEAQKEGPVPSEVIHSSVTPSKSPVMPSDSILPIRIRQDTTVYIQGIPY
                     DLTDAEATKIA"
BASE COUNT          460 a          557 c          601 g          422 t
ORIGIN      
        1 aacgaccgcg gcgaaggctg tttcctcaac atcgcgctgg gcgtcgtggg tgccttggtc
       61 ggcggcggta tctttgcgct cttgggcgaa ccggttttct tccgcttcag cctgtggtcg
      121 atgtttgtcg ccgttctggg cgcggtgatc gtgctctatt tgtggcacgc cgtgacggga
      181 cgacgccgat tgcgttgaat acgactcgca acagaaaagc gaatccgctc cgccgacgcg
      241 ccaatggtgc tatattccgg ccgcaatccg gaggcgccgc catgttcagg accgccgtta
      301 ccgccgcgtt tctcgccgct ttcgttggcc aagccaatgc ggccgccatc accgactgga
      361 atcaaatcgc caaagtgttc gataccattc cctcagccgc gggaatttcc gtgcacaagc
      421 gcatggacaa atgcggcgtc aagatcgtga aggacaaatc ctggatggaa acccgcgccg
      481 tcgattggcc ggaatatggc gcacgggccg gtgaaaccgt tcttctgatc acgataaaga
      541 taccctccgc cgcgggccag ccgccttcgc tgcaacaagg cacaaaggcg atatggctca
      601 tttcccgcgg caagccgatg tcggtgaatg cctgggccca gcaattccag agccgtacgg
      661 ttccgatggg ctacgacgcc tggatgaatt gttgaaatag acctccgtca gtcctaacgc
      721 gaggcttgtg cgatacacat aatttgtgta tgatctaaat ccgcatggct gaaagcatct
      781 ccagccgcga gatcatgtgc ctgctcgaaa aggcgggctg gcaggaagtg cggacgaccg
      841 gcagccatca tcattttcgc catgcgaaac ggctgggaac ggtgacggtg ccccatccgg
      901 tgaaagacat gccgcccgga accgtgaaaa gcatcgagcg gcagagcggc gtcaagttga
      961 ggtgagttat gcagcgtttc tatacggcaa tcatcgagaa ggcgaaggat ggatacggcg
     1021 tgttcttccc cgaccttccc ggctgcacca gcttcgggcg cagcgtcgaa gaggtcgcga
     1081 gcaatgctta caccgccgcc caggcgcatg tggcgctgtc gcgggaatat ggcgatgcga
     1141 ttgccgagcc gcgcgcgatg gacgcaatcc cggccgaacg cggcgtgaag gaaaaggcgc
     1201 gattgctcat tccggtggaa atggccgacg aaccggtgcg ggtgaatatc tcgctgccaa
     1261 gttcggcgtt ggaagcgctg gaccgcacag cgaaggaatt gtcgcttacg cgatcaggcg
     1321 cgattgcgta tctggcgcta gatagggatt caaggactga ccaagtagca ggacgagcca
     1381 agatgaagcc acgtaagagg tcggtgacat cagtgtaatc tcaaaatgca gggagcatct
     1441 ttgtgtacgt gaaattttct atgagaaatt tcttgacatt ttgattgtga ttcgatatct
     1501 tccagacata tctggaagga ggccactatg gccgatgatc gctcaagaca gaagcttatg
     1561 gaattcctgg agtatttggc caacaagggc ttgatggcgg aaaacacggt cgctgctcgc
     1621 aaatcagctg taagcaagat tttggggatt ctctcggaat ccgaagctgt ggacgtcacg
     1681 caacttgatc tggatcaagt gatggtccgc ttccagaacc tggaagggaa gaattatacg
     1741 ccaggcagca tgaataccta tttgagccgc ctgcgctctg caatcgacga ttttcgcacc
     1801 tatcttcaga acccgctcgc cttcaagccc agcgtgcaag ctcgcgagaa gcgcgcgaag
     1861 ccggaagcgc agaaagaggg acctgttcct tccgaagtta tccattcctc cgttacgccg
     1921 tcgaagtcgc ccgtcatgcc gtcggactcc atcctaccga ttcggatacg gcaagacacg
     1981 accgtgtata ttcagggtat cccatacgat ttgacggatg cggaggcgac caagatagcc
//
LOCUS       DASWPU010000206         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_69299,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000206 DASWPU010000000
VERSION     DASWPU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69299"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..528)
                     /locus_tag="VGC27_04490"
     CDS             complement(37..528)
                     /locus_tag="VGC27_04490"
                     /inference="COORDINATES: protein motif:HMM:NF025716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HEY0281865.1"
                     /translation="MNDFLRTYATPLSFVTFLGCTLTGLLLLFGIRSRPLGEVHEWLG
                     IVFVVALVLHLARNWRGVTVMLTAPRARLATGIGAVLVALYILSAAPFQSGHGHGRNI
                     TEMPIARMAPALGLSSDEAVARLKRGGVPVSGPQDSLQHLVHEHGQNMPRLLELLVSD
                     KPA"
     gene            complement(770..1999)
                     /locus_tag="VGC27_04495"
     CDS             complement(770..1999)
                     /locus_tag="VGC27_04495"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0281866.1"
                     /translation="MSDMSKPLPKRFLSESLGRIQPSSTLAVTQKARELKAAGRDVIG
                     LGAGEPDFDTPDNIKDAAIAAIRRGETKYTAVEGIAELRQAIAGKFKRENGLDYIPAQ
                     TFVSAGGKSIIYNAMMATLNQGDEVICIAPYWVSYPDIVLLGGGVPVIVEAKIEDGFR
                     LTPEALARAITPKTKWLIFNQPSNPTGACYTREQLKALTDVLLDHPHVWVLSDDMYEH
                     LVYGGFKFWTVAQVEPKLMERTLTMNGVSKAYAMTGWRIGYCACPEPLVKAMAKLQSQ
                     SVTNATSISQWASVEALNGTQDFIPKFQKAFEERRDLVVSILNQASGIECPKPEGAFY
                     VYPSIRKLIGKTAPSGKTIATDGDFAGELLEAEGVAVVHGAAFGLSPFFRISYATSNK
                     LLEEACTRIQRFCAGLK"
     gene            2150..2596
                     /locus_tag="VGC27_04500"
     CDS             2150..2596
                     /locus_tag="VGC27_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281867.1"
                     /translation="MRRFAVLLLIGLGMLIACSRPAQPPVGRWIGNYDAPDVMVDVRL
                     EITKDGLVRASAPDLLDVGKVSDETSAALRARLASDLAENWGNAELRVLDFDGRVFRK
                     PGGVAPQMEWNPKTRQMTLVFYFGTKRSIRIPMRAVENFDHDPWAR"
BASE COUNT          611 a         1013 c          988 g          616 t
ORIGIN      
        1 gatatagcgg atgctgcgaa cttcgaaacc acgacactag gccggcttgt cgctgaccag
       61 caattccagc aagcgcggca tgttctggcc gtgctcgtgc accaggtgct gcagcgaatc
      121 ctgcggccca gacacgggca cgccgccgcg cttcagccgc gccacggctt cgtcgctgga
      181 aagcccgagc gcgggcgcca tgcgggcgat cggcatctcg gtgatgttcc taccgtgccc
      241 gtgaccgctc tggaaaggcg cggcgctcag aatatagagg gccaccagaa ccgccccgat
      301 gccggtcgcc agcctggcgc gcggcgcggt cagcatgacg gtcacgcccc gccaattgcg
      361 cgccaggtgc aaaaccaggg caaccacgaa cacgatgccc agccattcat gcacttcgcc
      421 cagggggcgg ctgcggatgc cgaaaagcag caagagaccc gtcagggtac agcccaggaa
      481 ggtgacgaag ctcaagggtg tggcataggt gcgcagaaaa tcgttcatga ccggctccct
      541 ctagtgtcgt ggtttcgaag tttgcagcat gcgcactatc gggccgcaag cgaacttcga
      601 aaccaaaacg acactaggat tcaataggtt gctagtgtcc ttcgattccg aaattcgccc
      661 gaagccgata tagagatggg gcgaatttcg gaagcgggac actaggtgct ctccggcatt
      721 gaacgcgtac caccgccgct tccgttgcgg aaggcggcaa aaaatacgcc tatttcagcc
      781 cggcgcagaa gcgctgaatc cgcgtgcagg cttcctccag cagcttgttc gaggtcgcgt
      841 aggaaatacg gaagaagggc gacagtccga acgccgcgcc gtgcaccacc gccacgcctt
      901 cggcttccag cagttcgccc gcgaagtccc cgtccgtcgc gatcgtcttg cccgacggcg
      961 ccgtcttgcc gatcagcttg cggatcgagg gataaacata gaacgcgcct tccggcttgg
     1021 ggcattcgat gccgctggcc tggttcagta tcgagaccac cagatcgcgc cgctcctcga
     1081 acgccttctg gaatttcggg atgaagtcct gggtgccgtt gagcgcctcc acgctcgccc
     1141 attgcgagat cgacgtggcg ttggtcaccg attggctttg cagtttcgcc atcgctttga
     1201 ccagcggttc ggggcaggcg caatagccga tgcgccagcc ggtcatggca taggccttgg
     1261 acacgccgtt catcgtcagc gtgcgctcca tcagcttcgg ctcgacctgc gccaccgtcc
     1321 agaacttgaa gccgccatag accagatgtt cgtacatatc gtcgctcagc acccagacat
     1381 ggggatggtc cagcagcacg tcggtcagcg ccttcaattg ttcgcgcgtg tagcaggcac
     1441 cggtcggatt ggaaggctgg ttgaagatca gccatttggt cttcggcgtg atcgcccgcg
     1501 ccagcgcttc gggggtgagg cggaagccgt cctcgatctt cgcctcgacg atcaccggca
     1561 ccccgccgcc gagcagaacg atgtcgggat agctcaccca atagggcgcg atgcagatca
     1621 cctcgtcgcc ttggttcagc gtcgccatca tggcgttgta gatgatcgat ttgccgccgg
     1681 ccgagacgaa ggtctgcgcc gggatgtagt cgaggccgtt ctcgcgcttg aacttgccgg
     1741 cgatggcctg gcgcagttcc gcgatgcctt ccacggcggt gtatttggtc tcgccgcggc
     1801 ggatcgccgc gatggccgcg tccttgatgt tgtccggcgt gtcgaaatcg ggttcgcccg
     1861 cgcccaggcc gatcacgtcg cgcccggcgg ctttcagctc gcgcgccttc tgggttaccg
     1921 ccaaggtcga agacggctga atacggccga gcgactcgct taggaatctt ttcggaaggg
     1981 gtttggacat gtcggacatg ggagttcctg cgttccacgg ttgagccgct cacctatcaa
     2041 cccgccggcc gctacggcaa gacccgcaag agtagaaaag aacaccgagg cagctgttac
     2101 ctattctcaa cccggcccag ttagcctagg ccgcaagagg agtgtcttca tgcgccggtt
     2161 tgcggtcctt ctgttgattg gcttgggcat gctgatcgcc tgttcgcgcc cggcccagcc
     2221 gccggtcggg cgctggatcg gcaattacga cgcgcccgac gtcatggtcg atgtccgcct
     2281 cgaaatcacc aaggacgggc tggtgcgcgc cagtgcgccc gacttgctgg acgtcggcaa
     2341 agtatcggac gaaaccagtg ccgccctgcg cgcgcggctg gcctccgatc tggcggaaaa
     2401 ctggggcaat gcggaactga gagtgctgga tttcgacggc cgtgtcttcc gcaagccggg
     2461 cggcgtcgca ccgcaaatgg agtggaatcc gaagacccgg cagatgacgc tggtcttcta
     2521 tttcggcacc aaacgttcca tccgcattcc catgcgcgcc gtcgagaatt tcgaccacga
     2581 tccctgggcc cgctgaggcg cacgcttcat cattcgtgga aagcgaagaa agcggcgccc
     2641 gggtcaatct caccaaaatt tatatcgcga tgtctggcga cggcggcacc ccggcgacaa
     2701 tccgatacaa tattgcagcg ctgcgcactt tcgccggcgg gcgtggcgct tcaagctgtt
     2761 cccgcaatgg caacgacgcc ttccccgcgg atttgccaat gcgtcgcgaa cggtcccggc
     2821 accttcaatc cgatggcggg acagcgagcg atgttgaggc cgtgccccaa tcggcgctcc
     2881 acctgcgggc acagtctgat ggacggccgg atcccttcag ggcccccaag actgaagaag
     2941 gtccggccgc ctgcgcctct tgatttgaag atatagggat tttttcattg cgccgcggtt
     3001 cagaaattcc tgttgttcgc agggaaacag cccccatatt ggcgttcagt ggtcgctcct
     3061 gccataaggg cgacgcaaaa gagccgcagt cgccaaagca gtgagcattc ttatgagaat
     3121 cattcttaat tctgtgtcca tagcggttct agcactggct accgccggtt gtgcgacgtc
     3181 tccgccggtg gcgatgaaga cgagcgaact gccccaagcc tacaccgc
//
LOCUS       DASWPU010000207         3735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_112050,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000207 DASWPU010000000
VERSION     DASWPU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112050"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VGC27_04505"
     CDS             <1..768
                     /locus_tag="VGC27_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281868.1"
                     /translation="YRDITSRIFCTVEGASQGLERVGRLSILRTITEDSQSNSGFAFV
                     RAENEEISANEFSFMHLNGGWHLGSRKSRFGHIYGLIVNAAEEEELEDLDPSEQWETN
                     PDTAAVLDRVEAAHVIPPSRRGMRVVRGLYRRTDDEQCRFTISPFVVARSMDWRHQHR
                     ILICSVVTEQVHLRRAEDAELLRFLLEAPRSEEEMWKFCSQRGLASRPALRKTVEQLK
                     EDGILVICDMPDARDIRSVARDTAKAPEPLRESVGAA"
     gene            complement(785..1087)
                     /locus_tag="VGC27_04510"
     CDS             complement(785..1087)
                     /locus_tag="VGC27_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281869.1"
                     /translation="MLRNILLVLGGTLIASGLVALVGGMFAPSLIAIVWGGILVFGIL
                     YERYVYKTIADKPPAGKHWVKTTERFIDDKTGKTVTVHYNTLSGERVYVAEPAKAE"
     gene            complement(1088..1411)
                     /locus_tag="VGC27_04515"
     CDS             complement(1088..1411)
                     /locus_tag="VGC27_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEY0281870.1"
                     /translation="MLVATTENVPGYRRTKTLGQCFGLVVRSRGVGGNIIAGLRSIVG
                     GEIHEYTQLLEETRRQAVDRMVKNANAMGANAVVMMRFDSSEIGQIMSEIVAYGTAVV
                     LEEEK"
     gene            1548..1633
                     /locus_tag="VGC27_04520"
     tRNA            1548..1633
                     /locus_tag="VGC27_04520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1582..1584,aa:Leu,seq:cag)
     gene            2674..3522
                     /locus_tag="VGC27_04525"
     CDS             2674..3522
                     /locus_tag="VGC27_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0281871.1"
                     /translation="MSVHIRRFPAVTQEQPAPRRLTGVLLAGFSVLALSAPASADGDL
                     QVASAADSERLSAAALDAREEVVVTATRRAEPLQKVPLAVSVVDGDIAAFQNLININN
                     IATVVPSLNFRDGSSNKDQGLFVRGIGTVTTSPGAEPSVSTVVDGVVLVRPGQATLGL
                     FDVDHIEVLRGPQGTLFGKNASAGVVNIVTKQPTDEFLGFADLSYYEGNEVVARGGVS
                     GPLVKGKVDGLVSVLIGDYEGNVRNGFLNDTVNGYGRYGTRAKVAFTPSSDLKVVLSA
                     DYLYTH"
BASE COUNT          695 a         1143 c         1140 g          757 t
ORIGIN      
        1 taccgcgaca tcacttcgcg gatattctgc accgtcgaag gcgccagcca gggactggaa
       61 cgggtcggcc ggctttccat cctgcggacg attacggagg attcgcagtc caactccggc
      121 tttgctttcg tccgggccga aaacgaggaa atctcggcca acgaattcag cttcatgcac
      181 ctcaacggcg gctggcacct gggcagcaga aaatcccgct tcgggcatat ttacggcctg
      241 atcgtgaacg ccgcagagga agaggaactg gaggatttgg accccagcga gcagtgggag
      301 accaacccgg atactgccgc cgtcctcgac cgggtggagg cggcgcacgt cattccgccg
      361 tcacgccgcg ggatgcgggt cgtgcgcggc ctctaccgga gaacggacga tgagcaatgc
      421 cgctttacga tctcgccttt cgtcgtggcg cggtcgatgg actggcgtca tcagcaccgc
      481 atcctgatct gctccgtggt gaccgaacaa gtccatctgc ggcgggcgga agacgccgag
      541 cttttgcggt tcctgttgga ggcgccgcga agcgaagagg agatgtggaa attctgcagc
      601 caacgcggac tagcctcgcg acccgcgctg cgaaagaccg tcgagcagct caaggaagac
      661 ggcattctcg tgatctgcga catgccggac gcgcgggata tccgctccgt cgcgcgcgac
      721 acggccaagg ctccggagcc gctccgggaa agcgtcgggg cggcttgaag acacgcaaaa
      781 tccgtcattc ggccttggcg ggctccgcga cataaacccg ttcgccgctc aacgtattgt
      841 aatggaccgt cacagtcttg ccggtcttgt cgtcgatgaa gcgttccgtc gtcttcaccc
      901 agtgcttgcc ggccggcggc ttgtcggcga tcgtcttgta gacgtagcgc tcgtagagga
      961 tgccgaagac caggatcccg ccccagacga tggcgatcag cgagggtgcg aacatgccgc
     1021 cgacaagggc aaccagcccg ctggcgatca gcgtcccgcc caggaccagt aggatattgc
     1081 gcagcatcta tttctcttcc tcaagcacca cggccgtgcc gtaggcgacg atttcgctca
     1141 tgatctggcc gatctccgag gaatcgaagc gcatcatcac cacggcgttg gcgcccatgg
     1201 cgttggcgtt cttgaccatg cggtccacgg cctggcgccg cgtctcttcc aggagctggg
     1261 tgtattcgtg aatctccccg ccgacaatgg agcgcagtcc cgcgatgatg ttgccgccga
     1321 ccccgcgcga gcgcaccacc aggccgaaac attggccgag cgtcttggtg cggcggtatc
     1381 cgggcacgtt ttcggtggtg gccacgagca taggcggttc tccgggcgag tcccggccat
     1441 tgtagcgacc tgaaggcggc ttgtccggcc gtgccgcctt ctcatgcgtt gacttaaagc
     1501 tgtaccctcc ctaaacatcc cgcccccaag ggcttcggcc cttccgtgcc cagatggcgg
     1561 aattggtaga cgcactagtt tcaggtacta gcgctcacaa ggcgtggagg ttcgagtcct
     1621 cttctgggca ccaggacacc ttttgcgggt gtccgttgat atctgcttgg tgtaaaaaat
     1681 ccaaactttc cagtcgctta tcgtccgcca gcatccgccg gtgctctggt cctaccgccc
     1741 ggttttgtgg gaccgttgtg gtaccaagag tgcttcggtc ggggctgaac actcccaact
     1801 tatgggagtt cgacatgtct ttgacggacg tcacctgccg gtccaccaag gccgggtctc
     1861 aaacggcgcg agctgtccga cggcaacggt ctgcagctct ggatcatgcc cagcggcgcc
     1921 cggctttggc gcttcgtcta tcgttacctc ggacaacaga aggcctgggc gatcggcacc
     1981 tatcccgagg tgtcacttgc ggaagcgcgt gtgcagcgcg atgaggcgcg cctgttgctc
     2041 cgacagggaa aagacccgac cgcggagcgg gaccgaacgg ggaagcaacg aataggcaac
     2101 acacgcgagc gttcggctag ccttgtctcg ctaagccgtg cgacgtctgt ccgttacgcc
     2161 ggctcatgcc gcattcttgg tgcgtgatcc cttcggtgat tttccgcacc tcgacggtaa
     2221 tcacgagaat ttccgagaac gtcgccgcta gtggggctgt atgggcggag cgacatatcg
     2281 acttagttcc atattcgatt cgccaaatcg cacttctttc agagataaat cgcgccggat
     2341 tattatttca cagttcgcat cgcgtatttt ttggaattct gcaaaaatat tgactcgatc
     2401 aatttagtat acttatagtg ccgccgctgc accaaagtgt gggtggtagg ggccgccgaa
     2461 ggcttcgctt atggacggta tcagatcagt caatgtgatc ggtaccgtcc ttgttgcgta
     2521 gggaaaatat ggcgaagtgt cggagctgat gtagcgggcg cgatccgaag gtctcggcgc
     2581 tccgaggtgc gcgccgcctc ggtccgcatt tctcagagtg tcaacttgaa aactggtggc
     2641 cgcgggcatc accgccggcc aagagggggt actatgtctg tccacatacg tcgctttcca
     2701 gcggtcacgc aggaacaacc cgcaccgcgc cgcctgacgg gcgttctact ggcgggtttt
     2761 tctgtcctcg cattgtcggc gcccgcttcg gcggacggcg accttcaggt cgcgtcggcc
     2821 gccgatagtg aacgtttgtc cgccgctgcg ctcgatgcca gggaagaagt ggttgtaacg
     2881 gccacgcgcc gcgccgagcc gcttcagaaa gtcccgctgg cggtcagcgt cgtggacggg
     2941 gacatcgcgg cgttccagaa tctgatcaac atcaacaaca tcgcgaccgt tgttcccagc
     3001 ctcaacttcc gcgacggctc atccaacaag gaccagggtc ttttcgtccg cggcatcggc
     3061 acggtcacca cgtctccggg tgcggaaccg tcggtgtcca cggttgtgga cggtgtcgtt
     3121 ctggtgcgcc ccggtcaggc gacattgggc ctttttgatg tcgatcacat cgaagtcctg
     3181 cgcggaccgc agggaacgct gttcggcaag aacgcatcgg ccggtgtggt caacatcgtg
     3241 accaagcagc cgaccgacga attcctgggc ttcgccgatc tctcctacta cgaaggcaac
     3301 gaagtcgtgg cgcgcggcgg cgtttcgggg ccactggtga agggcaaggt ggacggcctc
     3361 gtcagcgtcc tgatcggcga ttatgaaggc aatgttcgca acggatttct gaacgacacc
     3421 gtcaacggtt acggccgcta cggcacgcgc gccaaggtcg ctttcacgcc gtcgtccgat
     3481 ctcaaggtgg ttctgtccgc cgattatctc tatacccact agaccggcgt cagcacgttt
     3541 gtcagcaccg aaacgaccta tcccggtggt gtgatcacgc ccgccccgag ctatgtcgcg
     3601 ttgctggcgc cggtggtggc aagccccggc aatacgcaga tcaacgccga ccagccaacc
     3661 tatgtcatcg acaacaacgc ggggttcagt gcccaggtcg attggagctt ctcggactac
     3721 acgctgacct cgatc
//
LOCUS       DASWPU010000208         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_69312,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000208 DASWPU010000000
VERSION     DASWPU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69312"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="priA"
                     /locus_tag="VGC27_04530"
     CDS             <1..1000
                     /gene="priA"
                     /locus_tag="VGC27_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY0281872.1"
                     /translation="AREPGTFLSPPLREALAATLGAGEQAMLFLNRRGYAPLTLCAAC
                     GHKMTCPNCSAWLVEHRYRKRLLCHHCGFETATPPQCPQCGEAGTLAACGPGVERVAE
                     EFHSVFPEARVAIASSDTMHGPAETQAAIRAMAKRETDVLIGTQIVAKGHHFPQLTLV
                     GVVDADLGGSDGDLRARERTFQLLHQVAGRAGRAEKPGLVLMQTRNPEDAVMRALASA
                     GRDAFYEQERKYHEAAHSPPFGRLAALILSGHDGDAVRSAGRALAKAAPSARGVKVWG
                     PTPAFYSLLRGQTRERLLVQAEKYVDIQAYLRAWLASATFAKSVRLTVDVDPVSFF"
     gene            complement(1014..1334)
                     /locus_tag="VGC27_04535"
     CDS             complement(1014..1334)
                     /locus_tag="VGC27_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281873.1"
                     /translation="MNVRVIAVGFAAFLLCGCSYWNSATTYFGLGPSEQSEPPLAYSA
                     AAEPNPPPIQADQAARFDEWCQRVAKSAAEEAAGNGYDSATQQRRAAKSYQQCRENPG
                     ASMR"
BASE COUNT          308 a          558 c          538 g          318 t
ORIGIN      
        1 agcgcgcgag cccggcacct tcctgtcgcc gcccttgcgc gaggcgctgg ccgcgacgct
       61 gggcgcgggc gaacaggcga tgctgttcct caaccggcgc ggctatgccc cgctgacgct
      121 gtgcgccgcc tgcggccata agatgacctg tcccaattgt tcggcctggc tggtggagca
      181 tcgttatcgc aagcggcttc tctgtcatca ttgcggcttc gagaccgcaa caccgccgca
      241 atgcccccaa tgcggcgaag cgggaacgct ggccgcctgc gggccgggcg tggagcgtgt
      301 ggcggaagaa tttcacagcg tgtttcccga ggcgcgtgtg gcgatcgcgt cttccgacac
      361 catgcacgga ccggccgaaa cccaagcggc gatccgcgcc atggcgaagc gcgagaccga
      421 cgttctaatc ggcacgcaaa ttgtggccaa aggccatcac ttcccgcaat tgacgttggt
      481 cggcgtggtc gatgcggacc ttggcggctc ggacggcgat ctgcgcgccc gcgagcggac
      541 ctttcaattg ctgcatcagg tggcgggacg cgcgggccgc gccgagaagc ccggtctggt
      601 gctgatgcag acgcgcaatc ccgaggacgc ggtgatgcgt gccctggcgt cggccggccg
      661 cgacgccttc tacgaacagg aacgcaaata ccacgaagcc gctcattccc cgcccttcgg
      721 gcgcctcgcc gcgctcatcc tgtcgggcca cgacggcgat gcggtgcgat ccgccggccg
      781 cgcgctggcg aaagcggcgc cttccgcacg cggggtcaaa gtgtggggac cgacgccagc
      841 cttctattcg ctgttgcgcg gccagacccg cgagcggctg ctggtgcagg cggaaaaata
      901 tgtcgatatc caggcttatc tgcgcgcctg gctggcttcg gcaaccttcg ccaaatcggt
      961 gcggctgacc gtggacgtcg atccggtgag ttttttctag tccggtcgat cggctaacgc
     1021 atggacgcgc cgggattctc ccggcactgc tggtaactct ttgcggccct tcgctgctgg
     1081 gtcgccgaat cgtagccatt gccggccgcc tcttcagcag cggacttggc cacccgttgg
     1141 caccactcgt cgaacctggc ggcctgatcc gcctgaattg gtgggggatt tggttcggcg
     1201 gcagccgaat aggctagtgg cggctcgctc tgttcggacg gtcccagccc gaaataggtt
     1261 gttgcactgt tccaatagga acagccgcaa agcaggaaag ccgcaaaacc cacggcgata
     1321 acgcgaacgt tcatggtgcc ccacctccaa aattcgttga accctagtgt cccgattccg
     1381 aaattcgccc gcagccgata tagagatggt gcgaatttcg gaatcgaaag aacactagca
     1441 acctattgaa tcctagtgtc gttttggttt cgaagttcgc ttgcagccag atagtgcgga
     1501 tggtgcaaac ttcgaaacca cgacactagc acaacttaca gacattgcga cgcgggagtc
     1561 ccggtgattt cccatccgcc aaaggtcgcg caaccgcagc agcggcgcac gatccccgtc
     1621 gtgccgcatg acgagcacgc cagcgccacg ccgcttcgat tccgcggcta acggccggaa
     1681 gatccaacgc attgccgata ggtcttttca gcccttcgct gc
//
LOCUS       DASWPU010000209         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_105923,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000209 DASWPU010000000
VERSION     DASWPU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105923"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VGC27_04540"
     CDS             complement(<1..1086)
                     /locus_tag="VGC27_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY0281874.1"
                     /translation="MADKPILTTTAGNKIADNQNAITAGPRGPVLMQDYQLLEKLAHQ
                     NRERIPERTVHAKGWGAFGTLTITSDITRYSKAAVFSAVGKKTPMLARFSTVAGEMGA
                     ADAERDVRGFALKFYTEEGNWDLVGNNTPVFFVRDPYKFPDFIHTQKRHPRTNMRSPT
                     AMWDFWSLSPESLHQVTILMSDRGLPTDVRHIDGFGSHTYSLINAANERFWVKFHFKT
                     KQGHKHYTNAEAEQVVGKTRESTQADLFGSIEKGDFPRWRFCVQIMPETDADKTPYNP
                     FDLTKVWPHSDYPLIEVGVMELNRNPDNYFTEIEQAAFSPSNIVPGIGLSPDKMLQAR
                     IFSYADAHRHRLGTHYEALPVNEPKCPV"
     gene            complement(1236..1640)
                     /locus_tag="VGC27_04545"
     CDS             complement(1236..1640)
                     /locus_tag="VGC27_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281875.1"
                     /translation="MLLALAAEDLASLSRAMRAAAAGCDGKGKLAAVSAAALSLLRNT
                     ETIAAASQALSATAGTSEAERLFNGRLIAALKILSDATGRNAGGRDAQCDVLLVAATL
                     SGLALLARAGRLEALGYTAEEVVERLEVRFAI"
     gene            complement(1692..1838)
                     /locus_tag="VGC27_04550"
     CDS             complement(1692..1838)
                     /locus_tag="VGC27_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281876.1"
                     /translation="MSESAAAVKRGERRAREHSATRASILEAARRVAARDGARDLSLR
                     GAAA"
     gene            complement(1882..2325)
                     /locus_tag="VGC27_04555"
     CDS             complement(1882..2325)
                     /locus_tag="VGC27_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281877.1"
                     /translation="MLKSCCFAAAILAAAAMPAFAEGECGSAPIAPAIPTASDLAGKT
                     VEAGRAEVVAAYKQVKVYQSALAPYRACLKSQDDADLAAIAEAKAKGAKEKDKIPPLQ
                     DRIAARLKLNNQTVDTEQQVATDFNDLHVAQCVNDTDPKICPKKK"
     gene            complement(2429..3043)
                     /locus_tag="VGC27_04560"
     CDS             complement(2429..3043)
                     /locus_tag="VGC27_04560"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0281878.1"
                     /translation="MTISIGILVFDGVEELDFVGPWEVFTMANEVRKQRGQGPVHDVT
                     LIAGSGRPVVCAKGMRVLPDLTTAQCAKLDVLLIPGGIGTRREATNRALLEWIATIAR
                     AAKWVTSVCTGVLLLTAAGPGKGKRVTTHWAFVETLRGRGDAAEVLDKFRYVRDGNMV
                     TAAGISAGIDMALWLTGQLHSPQFARAVQRGMEYDPAPPYAGLT"
BASE COUNT          575 a         1056 c         1058 g          679 t
ORIGIN      
        1 gaccgggcat ttcggctcgt tgaccggcaa cgcttcgtaa tgcgtaccga ggcgatggcg
       61 atgcgcgtcg gcataggaga agatgcgcgc ttgcagcatc ttgtccggcg ataagccgat
      121 gccgggaacg atattggacg ggctgaaggc cgcctgctcg atctcggtga aataattgtc
      181 ggggttgcgg ttcagttcca tcacgccgac ctcgatcagc ggatagtcgc tgtgcggcca
      241 caccttggtg aggtcgaaag gattataggg cgtcttgtcg gcgtcggtct caggcatgat
      301 ctgcacgcag aagcgccagc gcggaaaatc gcccttttcg atcgagccga aaagatcggc
      361 ctgggtcgat tcgcgggtct tgccgacgac ctgctcggcc tccgcattgg tgtagtgctt
      421 gtgaccttgc ttggtcttga agtggaactt gacccagaac cgctcgttcg ccgcgttgat
      481 gaggctgtag gtatggctgc cgaagccgtc gatatggcgc acgtcggtcg gcaggccgcg
      541 atcggacatc agaatcgtca cctgatgcag cgattccggc gacagcgacc agaaatccca
      601 catcgccgtc ggcgaacgca tgttggtgcg cggatgccgc ttctgggtat ggatgaaatc
      661 cgggaatttg tagggatcgc ggacgaaaaa taccggcgta ttgttgccga ccaggtccca
      721 gttgccttcc tcggtataga atttgagcgc aaagccgcgc acgtcgcgtt cggcatcggc
      781 ggcgcccatc tctccggcga cggtggaaaa acgcgccagc atcggcgttt tctttccaac
      841 cgcggaaaag accgccgcct tgctgtagcg cgtgatatcg ctcgtgatcg tcaaggtgcc
      901 gaaggcgccc cagcccttgg catgcacggt gcgttcggga atccgttcgc ggttctggtg
      961 ggcgagtttt tcgagaagct ggtaatcctg catcagcact gggccgcgcg gcccggcagt
     1021 tatcgcattc tggttgtccg cgattttatt acctgcggta gttgtaagaa ttggtttgtc
     1081 ggccatgtcg tgttcctttc gggtactgtc ctccgccttg agcgaatcct acattagaat
     1141 aattataaat tataagacgt ttcctttcct tacggacgcg catcttgtcg cagagcggcc
     1201 ctgccctgcg atcctctccg gcggcacggg tttcgtcaga ttgcgaaccg aacctctaac
     1261 cgctcgacga cctcttcggc ggtatagccc aacgcctcca ggcggcctgc ccgtgccagc
     1321 agcgccaagc cgctcagagt ggccgccacc agcagcacat cgcactgggc gtcgcggccg
     1381 ccggcgttcc ggccggttgc atccgagagt attttaaggg ccgcgatcag ccttccgttg
     1441 aacagccgtt ccgcttcgct ggttccggca gtggcggaca gcgcctggga agcggcagcg
     1501 atcgtctcgg tgttccgaag cagcgacagc gccgcagccg aaacggcggc gagcttgccc
     1561 ttgccgtcgc accccgcggc ggcggcgcgc atggcgcggg agagcgaggc cagatcctcc
     1621 gccgccagcg ccagcagcaa ttcgtttttg cttgcgaaat agccgtaaag cgccgccggc
     1681 gcgaatccgg cttaggctgc cgcgccgcgc agcgacaggt cgcgcgcgcc gtcgcgggcg
     1741 gcaacgcggc gcgccgcttc caggatggag gcgcgcgtcg cactgtgttc gcgcgcgcgc
     1801 cgttcacccc gtttgactgc cgccgctgac tcgctcatcg cccgacatga taggtgattc
     1861 gccgggcgga gcgtgtgcct gttatttctt cttcggacag attttcgggt ctgtgtcgtt
     1921 gacgcactgc gcgacgtgca ggtcgttgaa gtcggtggcg acctgctgct cggtatcgac
     1981 cgtctggttg ttcaacttca gacgggcggc gatgcgatcc tggaggggcg ggatcttgtc
     2041 cttttccttg gcgcccttgg ccttcgcctc ggcgatggcc gccaaatcgg cgtcatcctg
     2101 cgatttcagg cacgcccggt acggcgccag cgcgctctga tagactttga cttgtttata
     2161 ggcggccacc acttcggccc ggccggcctc gaccgtcttg ccggcaagat cgctcgcggt
     2221 cgggatagcc ggcgcgatcg gggccgaacc gcactcgcct tcggcaaacg ccggcatggc
     2281 cgccgccgcc aggatcgccg ctgcaaaaca acaactcttc aacatatttc tgagactccc
     2341 ctgagagaac gatgcccaac cggtccgaga taagcacatc tttccgggcg gaacaaaggc
     2401 aggtggcgga acaaaagcag gtgcgacatc aggttaagcc ggcgtaaggc ggggcgggat
     2461 cgtattccat gccgcgctga accgcccgcg cgaactgcgg cgagtgcagt tgccccgtca
     2521 gccacagcgc catgtcgatt ccggcggaga taccggctgc tgtaaccatg tttccgtcac
     2581 gcacgtaacg gaatttgtcg agcacttcgg cggcgtcgcc acggccgcgc aaggtttcca
     2641 cgaaagccca atgggtcgtc acccgcttgc ctttccccgg tcccgccgcc gtcagcagca
     2701 gaacgccggt gcagacactg gtcacccatt tcgccgcgcg ggcaatcgtc gcgatccatt
     2761 cgagcagcgc ccgattggtt gcctcgcgcc tggtgccgat gccgccggga atcaacagga
     2821 cgtcgagttt cgcgcattgg gcggtggtca gatcgggcag gacgcgcatt cccttggcgc
     2881 agaccaccgg cctgccgctc ccggcgatca gcgtgacgtc atggaccggc ccttggccgc
     2941 gctgcttgcg cacctcgttg gccattgtga acacctccca aggccctacg aagtcgagtt
     3001 cttccactcc gtcgaagacg agtattccga tgctgatggt catgcctgct cccgatgcgc
     3061 tgttgtccgg aacgatgtcc gctcagatag gatcgcgcca ctctcaccct tcgacaagct
     3121 cagggtgaag ggatttccga acccttcatg ctggtcggca cgctgttgct accgactgtc
     3181 gaagcatgag cttgcccgcc cgccggagct ttagcgcagg cgggtcgaag ggtgagcggg
     3241 acgattcaat cggattggat catggaaggc ttatgcagcg ccacggccaa gccctccacc
     3301 ggcgtgccgg cttccgtgcg cgggctgcat gccaagaggc ccgcgatctc caatccgttg
     3361 gcgtgcgc
//
LOCUS       DASWPU010000210         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_44808,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000210 DASWPU010000000
VERSION     DASWPU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44808"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VGC27_04565"
     CDS             <1..244
                     /locus_tag="VGC27_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0281879.1"
                     /translation="RCEMQVAKWGNSLAVRLPRSVVEILGLKEGDQIEITVSGARRLG
                     VGRDKSVEEALKKLRKYRGMMPKGFKFDREETHERR"
     gene            231..632
                     /locus_tag="VGC27_04570"
     CDS             231..632
                     /locus_tag="VGC27_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0281880.1"
                     /translation="MSAADFFDTNVLLYLVSPDSAKADRVEALLAQRGTISIQVLDEF
                     ASVATRKFGKKLSEVREVLSLIRLVCEVKAANIETHDLGLDIAERCKFSVYDSMLLAA
                     AIQARCSTFYTEDLQHGRKLEGLTIRNPFTS"
     gene            complement(687..1868)
                     /locus_tag="VGC27_04575"
     CDS             complement(687..1868)
                     /locus_tag="VGC27_04575"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0281881.1"
                     /translation="MKSGELLILSAVRTPMGGFQGELSACSGPRLGAAAIAAAIARAG
                     LEAGQIGEAVMGCVLAAGVGQAPARQAARFAGLPDSVPATTVNKVCGSGMKAIMIAAD
                     QLALGRVEIAVAGGMESMSNAPYLLLKARGGYRLGHGEVKDHMFLDGLEDAYENRLMG
                     TYAEDAARHYHFTREQQDAYATESLMRAKRAQADGTFAEEIVAVKVPIRSGEAEVALD
                     EQPRKADPAKIPTLKPAFAKDGTVTAANSSSISDGAAALVLARDDVAELRGLKPVARI
                     VAQASFAHEPRWFTTAPVGAIQKVLALAGWKAGEVDLFEINEAFANVAMIAMRDLSLP
                     HEKVNVNGGACALGHPIGASGARIVVTLLNALKRGKLKRGVAALCIGGGEATALAVEM
                     L"
     gene            1999..2916
                     /locus_tag="VGC27_04580"
     CDS             1999..2916
                     /locus_tag="VGC27_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281882.1"
                     /translation="MRKIAVSCAIGVGAGLALASAYLGAPYAGVYLTPAAVIVSAAVV
                     AFGSSIALLTFAGLSKPKTEKEKSKLKFSDLGPLDYAFFPPRDTDAAPIIGPDTDIHK
                     LAIVQNPSAYADKELMLIFKKGSFSKIGVHPIVVTEKESMLGWMKSFSRKADFNPTDI
                     RNLFMVLKDCKKLNHILLVNEHDEYIGYIPHEAVWDLTHENPESNIVEYIVDVLKNPN
                     KSGLLRALGGSSVEDYVFDTMLLSEGLEKLQERFASGLVVLKGNRNRKAIGVIYGHGL
                     MTVMLKGPAKKPETPVLAEPAAPDNARAL"
     gene            3176..>3303
                     /locus_tag="VGC27_04585"
     CDS             3176..>3303
                     /locus_tag="VGC27_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0281883.1"
                     /translation="MSSQRKVAIVTGAGQGIGRAIAIRLARDGFAIGCLDFNADTA"
BASE COUNT          664 a         1014 c          973 g          652 t
ORIGIN      
        1 gaggtgtgag atgcaggtcg ccaaatgggg gaacagcctc gccgtcaggc tgcctcgatc
       61 cgtcgtggag attctggggt tgaaggaagg cgaccagatc gagattaccg tctcgggggc
      121 gcgccgcctc ggcgtcgggc gcgacaagtc ggtcgaagag gcattgaaga agctgcgcaa
      181 gtatcgcggg atgatgccca aaggattcaa attcgaccgc gaagagaccc atgagcgccg
      241 ctgacttctt cgacaccaat gtccttctct acctcgtatc gccggactcc gccaaagccg
      301 atcgcgtcga agcgttgttg gcccaacgcg gaacgataag cattcaggtc ctggacgagt
      361 tcgcgtccgt cgcgacacgc aagtttggaa agaagttgag cgaggtccgc gaagtccttt
      421 cgctcattcg cctggtttgc gaggtcaagg ctgcaaatat tgaaacgcac gatcttgggc
      481 tcgacattgc cgagcgatgc aagttttctg tttacgattc gatgctgctg gcggctgcaa
      541 tccaggcccg ctgctcgacg ttctacacgg aagacctgca acatggccga aagctcgaag
      601 ggctgacaat ccgcaatcct tttacctcct aactaccttc ggcaccggcg cgttaaggca
      661 agcggatagc gctagtccaa cctccctcac agcatctcca ccgccagcgc cgtcgcttcg
      721 ccgccgccga tgcagagcgc agccacgccg cgtttcaact tgccccgctt caaggcgttc
      781 agcaaggtca cgacgatgcg cgcgccggaa gcgccgatgg gatggccaag cgcgcaggct
      841 ccgccgttga cgttgacctt ttcgtgcggc agggaaagat cgcgcatggc gatcatcgcc
      901 acattggcga aggcttcgtt gatctcgaac agatcgactt cgcctgcctt ccagcccgcc
      961 aaagcaagga ccttttgaat ggcccccacg ggcgcggtgg tgaaccagcg cggttcgtgg
     1021 gcgaaactcg cctgggccac aatgcgcgcg accggcttca ggccgcgcaa ctccgcaacg
     1081 tcgtcccgcg ccagcaccag cgccgccgcg ccgtcggaga tcgaggaaga atttgccgcc
     1141 gtcacggtgc cgtccttggc gaaggcgggc ttgagcgtcg ggattttcgc cggatccgcc
     1201 ttgcgcggct gttcgtcgag tgcaacctcc gcttcgccgc tccgtatcgg cactttcacc
     1261 gccacgatct cttcggcgaa ggttccgtcg gcttgcgcgc gcttggctcg cattagggac
     1321 tccgtggcgt aagcgtcctg ctgttcacgg gtgaaatggt aatggcgcgc cgcgtcttcc
     1381 gcataggttc ccatcagccg gttttcgtag gcgtcctcca ggccgtcgag aaacatatgg
     1441 tccttcacct cgccgtgacc cagccgatag ccgccgcgcg ctttcagcag cagatagggt
     1501 gcgttcgaca tgctctccat gccgcccgcc acggcgatct ccacccggcc cagcgcgagc
     1561 tggtccgccg ctatcatgat ggccttcatc ccggaaccgc acactttgtt gaccgtggtg
     1621 gccggcacgc tgtccggcag gcccgcgaaa cgcgccgcct gccgtgcggg cgcctgcccg
     1681 acgccggccg ccagcacgca gcccatgacc gcttcgccga tttggccggc ttccaggccc
     1741 gcgcgtgcga tcgcggccgc gatagccgcc gcgccgagcc ggggcccgct acaggccgac
     1801 aactcgccct ggaacccgcc catgggcgtg cggacggcgg aaagaatcaa caattcgcca
     1861 gacttcattc gccgactctt tagtttccac cataaaatta acgcaaacgc ggattttcgg
     1921 ccactttgcc ctggtctcgg actgagttat cattaatcga acgttagggt tgctgggttg
     1981 gcggagtaag taggggcaat gagaaagatc gcggtttcat gcgcgatcgg cgttggtgct
     2041 gggctggcgc tggcgagtgc ctatcttggc gcgccctacg ccggtgtcta cctgacgccg
     2101 gcagcggtaa tcgtttccgc ggccgtcgtc gcgttcggtt cttccatcgc cctgctgacc
     2161 tttgccggac taagcaagcc gaagacggaa aaagaaaagt ccaagctcaa attcagcgac
     2221 ctggggcccc tggattacgc cttcttccct ccccgcgaca ccgacgcggc gcccataatc
     2281 ggccccgata cggacatcca taagctcgcc atcgtgcaaa atccatccgc ttacgcggat
     2341 aaggagctca tgctcatctt caagaaagga tccttcagca agatcggcgt ccacccgatc
     2401 gtggtgacgg aaaaggaatc gatgcttggg tggatgaaga gtttctcccg caaggcggac
     2461 ttcaacccga ccgatatccg caacttgttc atggtgttga aggactgcaa gaagctcaac
     2521 cacatcctgc tggtgaatga gcacgacgaa tatataggct atattccgca cgaggcggta
     2581 tgggatttga cccacgaaaa tcccgaatcg aatatcgtcg agtacattgt ggatgtgctg
     2641 aagaacccca acaaaagtgg cttgttgcgc gcgctcggcg gttcttcggt cgaggattac
     2701 gtgttcgaca ccatgctgct gtcggagggg ctggaaaagt tacaagaacg tttcgccagc
     2761 ggcctcgtgg tgctgaaggg aaatcgcaac cgcaaagcca tcggcgtgat ttacgggcat
     2821 ggcttgatga cggtgatgtt gaagggcccc gccaaaaagc ccgagacacc ggtgctggcc
     2881 gaacccgcgg cccctgataa cgcgcgcgct ttataatttt ctcggacccg gctatcgggg
     2941 atgaccgcgg cctttaaggc tgccgacaaa ttcaacgact gacctgccaa ccgaagctgc
     3001 acgaatatag taatgtccgc gaccttcgac cattcgtcga ggcgtcagga acgccggagt
     3061 ttcttgctta atcaggtttt aatgacggca ggcggcctaa atattaacag gctcccctca
     3121 taaacgagga cattaaaggg ggaggttcgt gaatcctaac ccagaggaag atgccatgag
     3181 cagtcagcgc aaagttgcta tcgttacggg tgccggacag ggcatcggcc gggctatcgc
     3241 gatccgtctc gctcgggatg gctttgccat cggctgtctg gatttcaatg ccgacaccgc
     3301 caa
//
LOCUS       DASWPU010000211         5025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_38812,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000211 DASWPU010000000
VERSION     DASWPU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38812"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VGC27_04590"
     CDS             <1..319
                     /locus_tag="VGC27_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281884.1"
                     /translation="TWIGSAVGITVACVAIAIVAFVTHTPIVMQAYIPVIFAFYGVAW
                     FVAGALARRGWMYVAAAVSYAAALLLAALTGNPLQVLAMGVALLLLLTAPGLMLLSEE
                     IRK"
     gene            316..633
                     /locus_tag="VGC27_04595"
     CDS             316..633
                     /locus_tag="VGC27_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY0281885.1"
                     /translation="MSDEFDIAGIDDIIHGRLRLGIMAYLSGGGTADFNELKARLQAS
                     DGNLSVHLRKLEEAGYVEIAKSFLGRKPLTRVTLTDKGRSAFVAYLDAIEKLLPRHAD
                     GNE"
     gene            complement(655..1128)
                     /gene="greA"
                     /locus_tag="VGC27_04600"
     CDS             complement(655..1128)
                     /gene="greA"
                     /locus_tag="VGC27_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY0281886.1"
                     /translation="MERIPMTAAGYKALEEEINQLKTVERHAIIKLISEARAHGDLSE
                     NAEYHAAKERQAFIEGRVIELEDKIGRADVIDVSKLSGTTVTFGATVTMADEDTKEKR
                     TYQIVGDLEADAKKGRISISSPIARALIGKSKGDTVEVAAPGGARSYEILKVEWI"
     gene            complement(1335..4700)
                     /gene="carB"
                     /locus_tag="VGC27_04605"
     CDS             complement(1335..4700)
                     /gene="carB"
                     /locus_tag="VGC27_04605"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY0281887.1"
                     /translation="MPKRLDIHTVLIIGAGPIVIGQACEFDYSGAQACKALRAEGYRV
                     VLVNSNPATIMTDPGLADATYIEPITPEFVEKIIVREMANVPPGRVFALLPTMGGQTA
                     LNTALSLRKRGVLEKYDIELIGASAEAIDKAEDRELFRQAMMKIGLDVPRGVTLKGYL
                     RHKKDSAGNFLYDDTNNPVMELSPEAFTHALEALDQVGLPAVIRPSFTLGGTGGGIAY
                     NREEFFEIVERGLDASPTNEVLIEESVLGWKEFELEVVRDKVDNAIVICSIENVDAMG
                     VHTGDSITVAPALTLTDKEYQRMRSASIAVLREIGVETGGSNVQFAINPEDGRMVLIE
                     MNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDELTNDITKVTPASFEPTIDYVVTK
                     IPRFAFEKFPGAEAILSTSMKSVGEVMAIGRTFAESLQKALRSLETGLTGLDEIPLEN
                     EKNTIRAALARAAPDRLRLAAQAMRQGFPFDEIAAITKYDPWFLREIETILAVEAEVR
                     ASGLPHDADGMRRLKSMGFSDARLATLAGIREKGVRAARHALGVRPVFKRIDTCAAEF
                     KALTPYMYSTYEAPVAGAAECESKPTGAKKVIILGGGPNRIGQGIEFDYCCCHAAFAL
                     SERGYETIMVNCNPETVSTDYDTSDRLYFEPLTAEDVLEIVAKETEAGTLAGVIVQFG
                     GQTPLKLANTLVEAGVPLLGTSADAIDLAEDRKRFQVLIHELGLEQPRNATALTAEEA
                     IEAARTIGYPVILRPSYVLGGRGMVVVANEEQLKATVQSGELFRISGDNPVLIDGFLD
                     RAIEIDVDAIADETGEVFIAGIMEHIEEAGVHSGDSACAIPPHSLKKTTIREVERQTV
                     AMARALKVRGLMNVQYAVQNGDEIYVLEVNPRASRTVPFVAKAIGLPVAAIAARVMAG
                     ETLASLPHSRTRSRRICVKEAVMPFARFPGVDTILGPEMRSTGEVMGQDESFGRAFAK
                     SQIAAGLKLPLAGKVFISVRDADKMDTIAPARTLVAMGFELFATRGTARALEAAGLPV
                     KPVNKVLEGRPHVVDAIKNGEVQLVFNTTQGARAISDSASIRRTALTMKVPYYTTMAG
                     AAAATEAIAALRAGTLDVRPLQEKSQSEDFLSD"
BASE COUNT          840 a         1573 c         1641 g          971 t
ORIGIN      
        1 gacgtggatc ggcagcgcgg tcggcatcac ggtcgcgtgt gtcgcgatcg ccatcgtcgc
       61 ctttgtcacg cacactccaa ttgtcatgca ggcgtatata ccggtgattt tcgccttcta
      121 cggggtggcg tggttcgttg ccggcgccct ggctcgccgc ggctggatgt atgttgccgc
      181 tgcggtgtcg tacgcggccg cgctgttgct ggcggcgctg acgggcaatc cgctgcaagt
      241 gctcgccatg ggcgtggcgt tgctgcttct gcttacggcc cccggtctca tgctgctgtc
      301 cgaagaaatc cgcaaatgag cgacgaattc gacatcgcag ggatcgacga catcatccac
      361 ggccgcttgc gcctggggat catggcctat ctctcgggcg gcggcacggc cgacttcaac
      421 gagttgaaag caaggctgca ggccagcgac ggcaatctgt cggtgcatct gcgcaagctg
      481 gaagaagccg gttatgtcga gatcgccaaa tccttcctcg gcaggaagcc gctgacgcgc
      541 gtgaccctga ccgacaaggg gcgcagcgcc ttcgtcgctt atctcgacgc catcgagaaa
      601 ctgctgccca ggcacgcaga cgggaacgag tgaaacgcgc tcgctgcgcg gcgttcagat
      661 ccactctact ttcagaatct cgtaggaccg cgcgccgccg ggggcggcga cttccaccgt
      721 atcgcccttg ctcttgccga tcagggcgcg cgcgatgggc gaagagatcg agatgcggcc
      781 tttcttggcg tccgcttcca gatcgccgac gatctggtag gtgcgttttt ctttggtgtc
      841 ctcgtcggcc atcgtgaccg tcgctccgaa ggtcaccgtc gtcccggaga gcttggaaac
      901 gtcgatcacg tcggcgcggc cgatcttgtc ctccagctcg atgacccggc cttcgatgaa
      961 agcttggcgc tccttggccg cgtgatattc cgcgttctcg gagaggtcgc catgggcgcg
     1021 cgcctccgag atcagcttga tgatcgcgtg acgctcgacc gttttgagct gattgatctc
     1081 ttcctccagg gccttatagc ccgcggcggt cataggtatg cgttccatgg cgtcacagaa
     1141 tcctgttatc ggcaaaaact tccccacccg ctcatgcagg ccgaatggcc ggaggcggca
     1201 gtgaaagaag cccgctttta ctatatgaac cgggtcatcc cagccaaaac ggggattgaa
     1261 agtctaaggc gggcagaaag ggactgcaag ggcaagggcc cccaaacggc cccaaccctt
     1321 ttgttaaccc cttgttaatc agacaggaaa tcttccgact gcgacttttc ctgcagcggg
     1381 cggacatcca gcgtgccggc tctgagggcc gcgatggctt cggtggcggc ggcggcaccg
     1441 gccatggttg tgtagtaggg caccttcata gtcagcgcgg tgcgccggat ggaggcggaa
     1501 tcgctgatcg cccgtgcgcc ctgggtggtg ttgaatacga gttgcacttc gccgttcttg
     1561 atcgcatcca cgacgtgcgg gcgcccttcc agaaccttgt tgaccggctt gaccggcagc
     1621 ccggcggctt ccagcgccct ggccgtgccg cgcgtcgcga acaactcgaa acccatggcg
     1681 acaagggtgc gtgccggcgc gatcgtgtcc atcttgtcgg catcgcgcac cgagatgaag
     1741 accttgcccg ccagggggag cttcagcccc gcagcgatct ggctcttggc gaaggcgcgg
     1801 ccgaaacttt cgtcttggcc catcacttcg ccggtggagc gcatttcggg gccgagaatc
     1861 gtgtccacgc ccggaaagcg cgcgaagggc atcacggctt ccttgacgca gatgcggcgc
     1921 gagcgcgtgc gcgaatgcgg cagcgacgcg agggtctcgc ccgccatgac ccgcgcggca
     1981 atggcggcga ccggcagacc gatcgccttg gcgacgaagg gcaccgtgcg gctggcgcgc
     2041 ggattgactt cgagaacgta gatctcgtcg ccgttctgga cggcgtactg cacgttcatc
     2101 agcccgcgca ccttcagcgc gcgggccatg gcgaccgtct gacgctcgac ttcccgtatc
     2161 gtcgtcttct tcagcgaatg gggcgggatg gcgcaggcgg aatcgccgga atgcacgccc
     2221 gcctcctcga tatgctccat gatgccggcg atgaaaacct cgcccgtctc gtcggcgatg
     2281 gcgtccacgt cgatttcgat ggcgcggtcg aggaagccgt cgatcagcac cgggttgtcg
     2341 cccgaaatgc ggaacagttc gcccgattgc accgtcgcct tgagctgctc ctcgttggcg
     2401 acgacaacca tgccgcgccc gcccagcaca taggacggcc gaaggatcac gggatagccg
     2461 atggtgcgcg ccgcttcgat ggcttcctct gcggtcaagg cggtggcgtt gcgcggctgc
     2521 tcgaggccga gttcgtggat cagcacctga aaacgcttgc ggtcttcggc taggtcgatg
     2581 gcgtcggccg aggtgccgag cagcggaacg ccggcctcga ccagagtgtt ggcgagcttg
     2641 agcggcgtct ggccgccgaa ctgcacgatg acgcccgcca gcgtgcccgc ttccgtttcc
     2701 ttggcgacga tctccagcac gtcctcggcg gtgagcggct cgaaatagag ccggtccgaa
     2761 gtgtcgtaat cggtcgagac ggtctcggga ttgcagttga ccatgatcgt ctcgtagccg
     2821 cgctccgaga gcgcgaaggc ggcatggcag cagcaataat cgaattcgat gccttggccg
     2881 atgcggttgg gaccgccgcc caggatgatg accttcttgg cgccggtggg cttgctttcg
     2941 cattccgccg cgcccgcgac cggagcttcg taggtcgaat acatgtaagg cgtcagcgcc
     3001 ttgaattccg ccgcgcaggt gtcgatgcgc ttgaagacgg ggcgcacgcc gagcgcatgg
     3061 cgcgccgcgc gaactccttt ttcccttatg ccggccaggg tggcgaggcg ggcgtcggag
     3121 aaccccatcg atttgaggcg gcgcatcccg tcggcgtcgt gaggcaggcc actcgcccgc
     3181 acctcggcct cgacggccag gatcgtctcg atttcgcgca ggaaccaggg atcgtatttg
     3241 gtgatcgccg cgatctcgtc gaaggggaag ccttggcgca tggcttgggc ggcgagccgc
     3301 agccggtcgg gcgcggcgcg ggccagggcc gcgcggatcg tgttcttttc gttttcgagc
     3361 gggatttcgt ccaggcccgt caggccggtt tccagcgagc gcagcgcttt ctgcagcgac
     3421 tccgcgaagg tgcgcccgat ggccatgact tcgccgaccg atttcatcga ggtggagagg
     3481 atcgcctccg cgccggggaa tttctcgaag gcgaagcgtg ggatcttggt caccacataa
     3541 tcgatggtcg gctcgaaaga ggcgggggtc accttggtga tgtcgttggt gagttcgtcc
     3601 agcgtgtagc cgaccgccag cttggccgcg atcttggcga tgggaaagcc ggtggctttc
     3661 gaggccagcg ccgaggagcg cgacacccgc gggttcatct cgatcaggac catgcgcccg
     3721 tcttccgggt tgatcgcgaa ctgcacgttg gagccgccgg tttccacccc gatctcgcgc
     3781 agcaccgcga tcgaggccga gcgcatgcgc tgatattcct tatcggtgag ggtgagggcg
     3841 ggggccacgg tgatagaatc gccggtatgc acgcccatcg catcgacgtt ctcgatcgag
     3901 cagatgacga tggcgttgtc gaccttgtcg cgcacgacct ccagctcgaa ttccttccag
     3961 ccgagaacgg actcctcgat cagcacttca ttggtgggcg aggcgtcgag gccccgttcg
     4021 acgatctcga agaactcttc gcggttatag gcgatgccgc cgccggtgcc gccgagcgtg
     4081 aaggaaggac ggatcacggc gggcaggccc acctgatcga gcgcttccaa cgcatgggtg
     4141 aaggcttcgg gcgacagctc catcaccgga ttgttggtgt cgtcgtaaag gaagttgccg
     4201 gccgaatcct tcttgtggcg cagatagccc ttgagggtga cgccgcgcgg cacgtcgagc
     4261 ccgatcttca tcatcgcctg gcggaacagc tcgcggtcct cggccttgtc gatggcctcg
     4321 gccgaggcgc cgatcagctc gatatcgtat ttctccaaca cgccgcgctt gcgcagcgac
     4381 agggcggtat tgagcgccgt ctgcccgccc atggtcggca gcagggcgaa aacccgtccc
     4441 ggcggcacat tcgccatttc gcggacgatg atcttttcca cgaactccgg cgtgatcggc
     4501 tcgatatagg tggcgtcggc cagccccgga tcggtcatga ttgtggccgg attggaattc
     4561 accaggacga cgcgatagcc ctcggcgcgc aacgccttgc aggcctgggc gccggaatag
     4621 tcgaactcgc aggcctggcc gatgacgatg ggcccggcgc cgatgatcag aacggtgtgg
     4681 atgtcgaggc gcttaggcat ctaccttcct tcgtcatagc cgccaccccg gtccgcgcga
     4741 agcgcggccg gagtgcaagc tcttgaggcg gccatccagt gagtacgcgt cggcgcgcgt
     4801 aaggactctg gatggccggg tcaagcccgg ccatgacggt tctgtgttgt tgtatttccg
     4861 tttgtgctcc aaagttccac tactcaccgt tacttcaaaa aaaagccctc gccggatacc
     4921 gggagggccg tccaaatcct gtcttcgacg tcttcatcgc agcgttatct cccacaccgg
     4981 acccgccgac gacaaccccg actcgactgg aaccttgtcg cagag
//
LOCUS       DASWPU010000212         7613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_32539,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000212 DASWPU010000000
VERSION     DASWPU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32539"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1353)
                     /gene="gcvT"
                     /locus_tag="VGC27_04610"
     CDS             complement(238..1353)
                     /gene="gcvT"
                     /locus_tag="VGC27_04610"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF010093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY0281888.1"
                     /translation="MTESQSLRRTPLYALHVELGAKMVPFAGYDMPVQYPTGILKEHL
                     HTRAKAGLFDTSHMGQAFLSGGDPARALERVTPADLVGLKEGMQRYGLLLNDEGGIKD
                     DFMTTRLKGEDALYLVVNAATKEADFAYIAERLKGLATLAPQPERALLALQGPAAEAA
                     LARHAPGIEALTFMKAARAEVAGAAAIVSRTGYTGEDGFEISIAAKDAEKVARALLKE
                     PEVLPIGLGARDSLRLEAGLCLYGHDMDETTDPVEANLVWAIGKRRRQDKDFPAAAKI
                     VDEILTGAARKRVGIRLEGRAPARDGSEIADKSGRVIGRITSGGFGPSLNAPVAMGTV
                     ETAFAADGTEIDLLVRGRRLAARVAPMPFVPHGYKRA"
     regulatory      complement(1590..1709)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(2030..3829)
                     /gene="argS"
                     /locus_tag="VGC27_04615"
     CDS             complement(2030..3829)
                     /gene="argS"
                     /locus_tag="VGC27_04615"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY0281889.1"
                     /translation="MTALAAELSAIAGQAFAAEGLSARFGQVQPSDRPDLAQFQCNGA
                     LAAAKLALRPAEGGTGGEQRANPRRIAQKIADRLKADPLFAKVEIAGPGFINLDIADA
                     ALAERAGAMAKDARLGAPEAGKGKTLVIDFGGPNVAKPMHVGHLRSAIIGDCLQRLFR
                     ADGWTVVSDVHLGDWGLQMGQLVSEIDRRGLAPVYFDAAFNGSYPDESPVTMEDLEEI
                     YPAAAAACKADPARLEEARRATAELQAGRPGYVALWRHFVKVSERGLNREYSSLGVSF
                     DLWNGEASIDPLIAPMIEDLKRRGLAEESDGALVVRVAEPGDKKEMPPLILLKSDGAV
                     LYGTTDLATIVDRVKSHDPDLILYVVDQRQHGHFEQVFRAARKAKLSGKAALEHAGFG
                     TMNGPDGKPFKTRAGGVMKLFDLIAMTTDEAQKRLAEAGLANDYPPEERDDIARKVGI
                     AALKFADLSNYRLTDYTFDLSRFTKFEGKTGPYLQYAAVRIRSILRKAEEEGLSSAPP
                     VVRSAEERTLVLQLLALADAMASAEDKRAPNILCDYAFTLAQYFSRFYAEHHILSERD
                     AGLRAARLGLCELTVDTLAKVLGLLGIEIPQRM"
     gene            complement(3826..4389)
                     /locus_tag="VGC27_04620"
     CDS             complement(3826..4389)
                     /locus_tag="VGC27_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281890.1"
                     /translation="MTYRLIRIASALALAGLLAGCGTHSSISSYFFSDKAILCPDAAV
                     LANTASLPAFSPAAEDDPSGVVYKIAMTNVVTRCSYDKRERTADTWLKISFQAQRPPG
                     DGETGYRVPYYVALTSGGEIVDKKIYWLDFKFSGRNASARGETVIDSTIVKYEKGKTA
                     YDYHLLVGFQLTKAQLDYNAKMGRYEP"
     gene            4545..5504
                     /gene="ispH"
                     /locus_tag="VGC27_04625"
                     /gene_synonym="lytB"
     CDS             4545..5504
                     /gene="ispH"
                     /locus_tag="VGC27_04625"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002190.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0281891.1"
                     /translation="MEQKGAQGPRITLLLAAPRGFCAGVKRAIRIVELALAKFGAPVY
                     VRHEIVHNRTVVERLEGMGAIFIDELDQAPDGARVIFSAHGVPKAVPAEAKRRNLFYF
                     NATCPLVSKVHVEAERHHAAGRHVILIGHAGHPEVEGTMGQLPKGGITLVETEADIAA
                     LKPADPAHLAFITQTTLSVDDTSGLVAALRRRFPRIAGPRKEDICYATTNRQQAIKQI
                     APRADLVLIIGAPNSSNSVRLVEVARRAGAKRAVLIENAEDIDWALLDGVKTVALSAG
                     ASAPETLVREVIDAFRARTDLTVEETTVTKEHVEFNVPRALAC"
     gene            5517..6476
                     /gene="thrB"
                     /locus_tag="VGC27_04630"
     CDS             5517..6476
                     /gene="thrB"
                     /locus_tag="VGC27_04630"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY0281892.1"
                     /translation="MAVYTEVSFEDLEVFLAEYDLGAPLSFKGIAEGVENSNYFLQTE
                     RGSFILTLYEKRVHAGDLPFFLGLLGYLSARGIACPLPVRSRNGAHSAHLNGRPAALL
                     TFLNGLSLRRPEAAHCAAAGTALAALHEAGKGFPLTRPNALGADGWSALVQACIPRAD
                     SVQDGLGALIETAHAELLARWPRDLPAGIIHADLFPDNVLFMNGAVSGLIDFYFACND
                     AYAYDLAVMLNAWCFESDGAYNLTKGKSLIAAYRRERTLSEAEIEALPVLAGGAALRF
                     LLTRLYDWLNHDPTALVRPKDPRDYAKRLRFHKQVKSAAEYGL"
     gene            6473..6916
                     /gene="rnhA"
                     /locus_tag="VGC27_04635"
     CDS             6473..6916
                     /gene="rnhA"
                     /locus_tag="VGC27_04635"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY0281893.1"
                     /translation="MSKTRVDIFTDGACSGNPGPGGWGAILRAGKHEKELSGGEPATT
                     NNRMELTAVIRALEALKQPSAVVVHTDSRYVMDGATKWMKNWKKNGWKTADKTPVKNE
                     ELWRALEDASARHAISWRWVRGHAGHAENERADALARAAIPGNGE"
     gene            complement(7057..7407)
                     /locus_tag="VGC27_04640"
     CDS             complement(7057..7407)
                     /locus_tag="VGC27_04640"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEY0281894.1"
                     /translation="MRDSKPKTVSRARVLRKRMTDAEVILWSRLRRGGANRLHFRKQH
                     PIGPYVADFACAQAHLVIEVDGDTHSTETELEHDRKRELYLKLHGWHVFRVTNEDVYK
                     RLDSVVEGIFLHIS"
BASE COUNT         1312 a         2503 c         2426 g         1372 t
ORIGIN      
        1 aattgctcgg tcgcatggcg ggtgatgccg acagtgcctt cgctaccttc gacgcgaacc
       61 cattcatgtt gatcggtgaa ccgtatctcg ctcattctcc acctcgttgc ccacatgctt
      121 tgaaaaactc atgcttcgac aagctcagca tgaggggtta actgaacgcc ttcaccctga
      181 gcttgtcgaa gggtgagctc gtcgaagggt gagcttgtcg aagggtgacg gcatatctca
      241 tgccctcttg tagccgtgcg gcacgaaggg catcggcgcc acgcgggcgg caagccgccg
      301 gccgcgcacc aacaggtcta tttccgtgcc gtcggccgcg aaggcggttt cgacagtgcc
      361 catcgcgacg ggcgcattca ggctcggccc gaagccgccg ctggtaatgc gtccgatgac
      421 gcggccggac ttgtcggcga tctcgctgcc gtcgcgcgcc ggagccctgc cttcgagacg
      481 gatgcccacg cgcttgcgcg cggcgccggt caaaatctcg tccacgatct ttgcagcggc
      541 gggaaaatcc ttgtcctgtc tgcggcgctt gccgatggcc cagaccagat tggcttccac
      601 cggatcggtg gtttcgtcca tgtcgtggcc gtaaaggcaa agtccggctt ccagccgcaa
      661 gctgtcgcgc gcgcccagac cgatgggcag aacttccggc tctttcagca gcgcccgcgc
      721 caccttctcg gcgtccttgg ccgcaatgga aatctcgaaa ccgtcttcgc cggtataacc
      781 ggtgcggctg acgatggccg ctgctcccgc cacttcggcg cgcgcggcct tcataaaggt
      841 aagcgcctcg ataccgggcg cgtgacgcgc cagcgcggcc tcggcggccg gtccttgcag
      901 cgccagcaag gcgcgttccg gctgcggcgc cagtgtggcc agacccttca gccgctcggc
      961 gatataggcg aaatcggctt ccttcgtcgc ggcgttgacg accagataaa gcgcgtcttc
     1021 gcccttgagc cgagtcgtca tgaaatcgtc cttgatgccg ccttcatcat tgagcagcag
     1081 gccgtagcgc tgcatgccct ccttcaggcc aacaaggtcg gcgggcgtca cccgctccag
     1141 cgcccgcgcc ggatcgccgc ccgagagaaa cgcctggccc atatgcgagg tgtcgaacag
     1201 cccggccttg gcgcgggtgt gcagatgttc cttgaggatg ccagtggggt actgcacggg
     1261 catgtcgtag cccgcgaacg gcaccatctt agcgcccagc tcgacatgca aggcatagag
     1321 cggcgtgcgg cgcagggact gtgattcggt catgggcggc ccggcaaaga ggttgatacg
     1381 gcgcgatcca tcgatctcgt tcgacggaac tcgcgccccc tctgtcgctg gacctgaggg
     1441 atttcccccg gacgcgctcc aaaagagccg gcccgggatt accccttcgg tgagggccgc
     1501 gaccgtcgca tgatccgcaa aagtggaacc cggttttgcg aaaagatcat gcgcaaacaa
     1561 aagtttgcgg ccctgctttc cagaagttca tccccttcgc ggtccttttg cctgagagtt
     1621 tccggggcgg ttgctccttc ggccttcgcc ttcggcttcg gccgacgggt cggccttcga
     1681 cgaagacaaa cgtctcccgc gagggatcgt ttgctcgccg cagcgggcgc gcctcgcggc
     1741 gggcccgtgc gttaggggca tcataggggg gaccataagg gagtcaacgt gggccgggat
     1801 agcggctcag ttttcgctct cgttcgtcat ggccgggctt gccgccttcg ctaaagctac
     1861 agcggccaag tgagcccgct cttgcccgcc gaagccttgg cgtaggcggg acccggccat
     1921 ccagtcgggc gcgtccgcga gccaaaaaac tctggatggc cgcctcaagg gcggccatga
     1981 cgaaggtaga tgcgaatgac gacgccattt cttcggcagt ccgattcact cacattcttt
     2041 gcgggatttc gatgcccaac aggcccagga ccttggccag cgtgtccacg gtcagctcgc
     2101 acagtccaag gcgggcggcg cgcaggccgg cgtcccgttc cgacaggatg tggtgctcgg
     2161 catagaagcg gctgaaatac tgcgccaggg tgaaagcgta gtcgcacaga atgttcggcg
     2221 cgcgcttgtc ctcggccgag gccatcgcgt cggcgagagc gagcaattgc aggaccagcg
     2281 tacgctcctc ggccgaacgc acgacgggcg gcgcggaaga aagcccttcc tcttccgcct
     2341 tgcgcaaaat cgaacggatg cgtaccgcgg cgtattgcag atagggtccg gtcttgccct
     2401 cgaacttggt gaaacgggag agatcgaagg tgtagtccgt cagccggtaa ttcgacaggt
     2461 cggcgaattt gagcgcggcg atccccacct tgcgggcgat gtcgtcgcgc tcctccggcg
     2521 gatagtcgtt cgccagcccg gcctcggcga ggcgcttttg cgcttcatcg gtggtcatgg
     2581 cgatgaggtc gaagagtttc atcaccccgc cggcgcgggt cttgaagggc ttgccgtcag
     2641 gcccattcat ggtgccgaag ccggcatgtt cgagcgcggc cttgccgctc agtttcgcct
     2701 tgcgcgcggc gcggaacacc tgctcgaaat ggccgtgctg gcgctgatcg accacataga
     2761 gaataaggtc cggatcgtgg cttttgacgc gatcgacgat ggtggcgagg tccgtcgtgc
     2821 cgtagagcac cgcgccgtcg gatttgagca ggatgagcgg cggcatctcc ttcttgtcgc
     2881 ccggctcggc cacccggacg acgagcgcgc cgtcgctctc ctccgccagg ccgcggcgct
     2941 tcaaatcctc gatcatcggc gcgatcaagg gatcgatgct ggcctcgccg ttccacaggt
     3001 cgaagctgac gccgaggctc gaatattccc ggttcaagcc ccgctcggag actttcacga
     3061 aatggcgcca cagcgcgaca tagccgggac ggcccgcctg caattcggcg gtggcgcgcc
     3121 gcgcctcctc cagccgcgcc ggatcggctt tgcaggcggc ggcggcggcc gggtaaatct
     3181 cctcaaggtc ctccatcgtc accggcgatt cgtccgggta cgaaccatta aaggccgcgt
     3241 cgaaatagac gggcgccagg ccgcgccggt cgatctcgct gacgagctgg cccatttgca
     3301 ggccccaatc gcccagatgc acgtcgctga ccacggtcca gccgtcggcg cggaacagcc
     3361 tctgcaggca gtcgccgatg atcgcggagc gcaaatggcc gacatgcatc ggcttggcga
     3421 cgttgggccc gccgaaatcg atgaccaggg tcttgccctt gcccgcttcc ggcgcgccca
     3481 gccgcgcgtc cttcgccatc gcgccggcgc gctcggccag cgccgcatcc gcgatgtcga
     3541 ggttgatgaa gccgggcccg gcgatctcga ccttggcgaa aagcggatcg gccttgaggc
     3601 gatcggcgat cttctgcgcg atccggcgcg ggttggcgcg ctgttccccc ccggtcccgc
     3661 cttcggcggg ccggagggca agcttcgccg ccgccagcgc gccgttgcac tggaactggg
     3721 ccagatcggg gcggtcggac ggctgcacct gcccgaaccg cgcggacagt ccctcggccg
     3781 cgaacgcctg ccccgcgatc gcgctcagct ccgccgcgag cgccgtcatg gctcgtagcg
     3841 ccccatcttg gcgttgtagt cgagctgcgc cttggtcaac tggaagccga ccagcaggtg
     3901 gtaatcgtag gccgttttgc ccttctcgta tttcacgatg gtggagtcga tcacggtctc
     3961 gccacgggcg gacgcattgc gcccggagaa cttgaaatcc agccagtaaa tcttcttgtc
     4021 gacgatctcg ccgcccgatg tcagcgcgac gtaatagggg acgcgatagc ccgtctcgcc
     4081 gtcgccgggc ggacgttgcg cctggaagga aatcttcagc caggtgtcgg cggttctttc
     4141 cctcttatcg tagctgcacc tggtcacgac attggtcatg gcgattttat agaccacgcc
     4201 cgaaggatcg tcctccgccg ccgggctgaa cgccggcagg ctcgcggtgt tcgccagcac
     4261 cgccgcatcg gggcaaagga tggccttgtc gctaaaaaag taggaggaga tcgaactatg
     4321 cgtcccgcaa ccggccagaa ggccggcgag cgccaacgcc gatgcaatgc ggatcaatcg
     4381 gtatgtcatg gagggatcac tacgccttgt tggtacggtt gcgccaggac cgtcgggccg
     4441 gccgctttct cgccttttgc ttgacttttc gtggctcgcg gccgtcttct ggatctgcgc
     4501 caaagagcgc cggactgtag ccaagcgggc tctttatggg aagcatggag caaaaggggg
     4561 cccagggacc gcggatcacg ctgctgctcg ccgccccgcg cggcttctgc gcgggtgtga
     4621 agcgggcgat tcgcatcgtc gaactggcgc tcgccaaatt tggcgctcct gtctatgtcc
     4681 gccacgaaat cgtccataac cgcaccgtgg tcgaacggct ggaggggatg ggggccatct
     4741 tcatcgacga gctggaccag gccccggacg gtgcccgggt catcttttcc gcgcatggcg
     4801 ttcccaaagc cgtgcccgcc gaagccaaaa ggcggaacct tttctatttt aacgcgacct
     4861 gcccgctggt ctcgaaggtg catgtggagg ccgagcgcca ccacgccgcc gggcgccacg
     4921 tcatcctgat cggtcacgcc ggccatcccg aagtggaagg cacgatgggc caattgccca
     4981 aaggcggcat caccctggtc gaaaccgagg ccgatatcgc ggcgctcaaa cctgccgatc
     5041 ccgcgcatct ggccttcatc acccagacca ccctgtcggt ggacgataca agcggactgg
     5101 ttgccgccct gcgccgccgc ttcccccgga tcgccggtcc gcgcaaggag gacatctgct
     5161 acgccaccac caaccgccag caggccatca agcagatcgc gccccgcgcc gatctggtgc
     5221 tgatcatcgg cgcaccgaat tcatcgaatt cggtcaggct ggtggaggtc gcccggcgcg
     5281 cgggcgccaa gcgcgccgtt ctcatcgaaa acgccgaaga catcgactgg gcgctgctcg
     5341 acggggtgaa gacggtggcg ctctcggccg gcgcttcggc acccgaaacg ctggtgcgcg
     5401 aggtgatcga cgccttccgc gcgcgcaccg acctcaccgt cgaagaaacc acagtcacca
     5461 aagaacatgt ggaattcaac gtgccccgcg cactcgcttg ctgaaggttc cagccgatgg
     5521 ccgtttatac cgaggtttcc tttgaagacc ttgaagtctt cctggcggag tacgatctgg
     5581 gagccccgct gagcttcaag ggcatcgccg aaggggtgga gaattccaac tatttcctcc
     5641 agaccgagcg cggctcgttc atccttacgc tgtacgaaaa acgcgtgcac gccggcgacc
     5701 tgcccttctt cctgggattg ctggggtatc tgtcggcgcg cggcattgcc tgtccgttgc
     5761 cggtgcgcag ccggaacggg gcgcattccg cccatctcaa cggccggccg gccgctttgc
     5821 tgaccttcct gaacgggctg tcgctgcgcc ggccggaagc cgcccattgc gccgcggcgg
     5881 gcacggccct ggcggccctg cacgaagcgg gcaaaggctt tcccctgacg cgccccaatg
     5941 cgctgggcgc ggacggctgg agcgcgctgg tgcaggcctg catcccgcgc gccgacagcg
     6001 tgcaggacgg cctcggcgcg ctgatcgaga cggcccacgc cgagcttctg gcaagatggc
     6061 cgcgcgatct gccggccgga atcatccatg ccgatttgtt tcccgacaac gtgctgttca
     6121 tgaacggcgc ggtctccggc ctgatcgact tctatttcgc ctgcaacgac gcctatgcct
     6181 acgatctggc cgtgatgttg aacgcctggt gcttcgaaag cgacggcgcc tacaacctca
     6241 ccaaaggcaa aagcctgatc gcggcctatc gccgggaacg aaccttgagc gaggccgaga
     6301 tcgaagcgct gccggtgctg gccgggggcg cggcgctgcg cttcctgctg acgcggctct
     6361 acgactggct gaaccacgac ccgaccgcgc tggtgcggcc caaggacccg cgcgattacg
     6421 ccaagcgcct gcgcttccac aaacaggtga agagcgccgc ggagtacggc ttgtgagcaa
     6481 aacgcgagtg gacatcttca ccgacggcgc ctgttcggga aatcccggtc ccggcggctg
     6541 gggggcgatc ctgcgcgcgg gcaagcatga gaaggaattg tcgggcggcg agccggcgac
     6601 caccaacaac cgcatggaac tgaccgccgt catccgggcg ctggaagcgc tgaaacagcc
     6661 gtccgccgtc gtggtgcata ccgattcccg ctatgtgatg gacggcgcca ccaagtggat
     6721 gaagaactgg aagaagaacg gctggaagac cgccgacaag acgccggtca agaacgagga
     6781 attgtggcgc gcgctggaag acgcctcggc gcgccatgcg atctcctggc gctgggtgcg
     6841 cggccatgcc ggacacgccg agaacgaacg cgccgacgcc ctggcgcgcg cggcgatacc
     6901 gggaaacggc gaatagcgaa tggcttttct gccccagcgt ttgtcacgcc gaagcggcga
     6961 tagccgcgaa agcgggaagc gaggggctgc cccccttcgg ccgcttcgcg gccacttccc
     7021 ccacttcgtg ggggcagaaa gtaagacgcc gatcaattat gatatatgca gaaatatccc
     7081 ctcgaccacg ctatcgaggc gtttgtagac atcctcgttc gtcacgcgga acacatgcca
     7141 gccgtgtaat ttcaaataca attcacgttt gcgatcatgt tccaattcgg tctcggtgga
     7201 gtgcgtatct ccatccacct cgatgacaag gtgcgcttgt gcgcaggcaa aatcggcaac
     7261 gtatggaccg atgggatgct gcttgcggaa gtgaagtctg tttgccccgc cccgtcttaa
     7321 gcgcgaccat agaatgactt ccgcatcggt catgcgtttg cgcaggacgc gggcgcggga
     7381 caccgttttg ggtttgctat cgcgcaaatg ctccccttcg cctcgcgtgc gctgtcgcta
     7441 cgcgccgctc ggcacttccc ccgcaaaagc gggggcagaa ccccccttcg gccgctgcgc
     7501 ggccgcttcc cccacttcgt gggggcagaa aacattcgcc ccttatatct gccccaggat
     7561 gtgctcggcg ctcgacaccg cgaacgcgcc gggctcctcg atgttgagct gct
//
LOCUS       DASWPU010000213         5649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_118127,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000213 DASWPU010000000
VERSION     DASWPU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118127"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VGC27_04645"
     CDS             <1..1140
                     /locus_tag="VGC27_04645"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY0281895.1"
                     /translation="TKDRGFTIVDWISDPIALARGDADIVVESIGGENGPALALVEAA
                     LKAGKHVVTANKAMMARHGARLAALAEERNLALKFEAAVAGGIPIVKSLRESLFVYGV
                     AAVRGILNGTCNYILTQMESTGRPFEDVLKDAQALGYAEADPTLDVGGGDTAHKLALL
                     AGLAFGVAPDLDTMAVQGITHIMPEDIAFAREFGFRIKLLGIAKKTPQGIDQRVQPAM
                     VRAGTPLGDVDGVYNAVSADTGAAGYFFFEGRGAGEAPTANAVIADIVDIARGNIGPV
                     FGIQARSLIPLTPAPGGSASSAFYLRFQVLDVPGVLAEIAGHLAKWRVSIESMIQRGR
                     APGEAVSIVMITHETTQAAVDCALKTIAASDKIISPPCMIPMEAK"
     gene            1169..2128
                     /gene="glpX"
                     /locus_tag="VGC27_04650"
     CDS             1169..2128
                     /gene="glpX"
                     /locus_tag="VGC27_04650"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY0281896.1"
                     /translation="MAGRLLDRAFALETVRITEAAAVAASEFVGRGDEHAADRATVEA
                     MRKTFNYLPINGTVVIGEGERDEAPMLYIGEKVGCGGVDVDIALDPLEGTALTAKALP
                     NALAIVAMAEPGTLLNAPDTYMDKIAIGGGYPEGTIDLDAEPAENVLALAKAKNVPPR
                     EINVCILDRPRHETHIASVRSAGGSVRLITDGDVAGIIATSDPATGIDMCLGRGGAPE
                     GVLAAAALRCVGGQMQARLFFKSDDEKKRATKLGIRDFDRKYTLMDLASGDVIFAATG
                     VTDGAMLQGVYRTPEFVFTHSVVMRSASGTVRWIKARHRRHEH"
     gene            2118..3923
                     /gene="recJ"
                     /locus_tag="VGC27_04655"
     CDS             2118..3923
                     /gene="recJ"
                     /locus_tag="VGC27_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY0281897.1"
                     /translation="MSIDAHRAGGRTPSQEGARSLLGVSLSFSGRRWIVPQSDEDAIR
                     AMAEHDIPLALARVLAARGVSANEAADYLNPTLKRLLPEPLTLKDMDKAVARVAAAVA
                     NGERIAVFGDYDVDGSCSAALLHDFLASVSAPPRLYIPDRMSEGYGPNAAALLRLKAE
                     GIGLVVTVDCGAGAADALTVAREAGLDVIVLDHHAVEREPPCVAHVNPNRHGDTSGLG
                     HVCAAGIVFLFLVALNRALREAGRYARKAEPDLRDALDLVGLATVCDVVPLIGVNRAF
                     VQTGLARLGKLERPGLAALAAIARVAPPFTPYHLGFILGPRINAGGRVGRCSLGADLL
                     TAKGPEAEEFAAALDSHNRERQAIEKLILDEAMAQAAAQDNQPFLFLAGDGWHPGVVG
                     IVAGRLKERFGKPAFVAGFEGGLGRGSARSIAGVDIGAVVRAAREAGTLDAGGGHAMA
                     AGFSLRPEQTDPFRAFLAAEFAKFSDTSALVATLTLEAVVSPAGATPALVHDIGRAGP
                     FGAGNPEPLLAVPDAEVAFADIVGADHVRLRLTGGDGARLDAIAFRAADTDLGRALLG
                     ARGKRIHAAGRLRADEWNGRTRVQLHLDDAAPADA"
     gene            3998..4072
                     /locus_tag="VGC27_04660"
     tRNA            3998..4072
                     /locus_tag="VGC27_04660"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4031..4033,aa:Glu,seq:ttc)
     gene            4481..>5649
                     /locus_tag="VGC27_04665"
     CDS             4481..>5649
                     /locus_tag="VGC27_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY0281898.1"
                     /translation="MTWSSSQKQKSVGMNLTLDRLRLVFVDGEQEKAFVREALIASIG
                     FIRAYLIAGTGLYMVFGLLDALVGAQSVQALWIVRYAVVCPLLIGVFVLTFFPVFARI
                     GQYALGSTMLVSGFGIIVMTAIMPPPFNAMYYAGLVMVVIYCSSLIRLQFNLSAAIST
                     FLVVCYQIVAIWINPVPLTTFISNDFFLIMATAVGLFSGYIQELYIRRSYISQKVIEA
                     KNEALNVLLVEADKANKSKSEFLANMSHELRTPLNAIIGFSDILKKQLFGPLGSNRYQ
                     EYVKDINDSGAHLLDIINDILDLAKAEAGKLELQPGEHDLAECLDDCIRMCRARAESC
                     GVRLTLGEVASPVLAFVDRRLIFQALLNLATNGIKFTPAGGTVTLSMVCPEQRGV"
BASE COUNT         1001 a         1762 c         1829 g         1057 t
ORIGIN      
        1 accaaggacc gcggcttcac catcgtcgat tggatcagcg atccgattgc tctggcgcgc
       61 ggcgatgccg atatcgtggt ggaatcgatc ggcggggaaa acgggccggc gctggccttg
      121 gtcgaagcgg cgctgaaagc cggcaagcat gtcgtgacgg ccaacaaggc gatgatggcg
      181 cgccacggcg cccggctggc cgcgctggcg gaagagagaa accttgcgct caaattcgag
      241 gcggcggtcg ccggcggcat ccccatcgtc aagtcgctgc gcgagagcct gttcgtgtac
      301 ggcgtggccg ccgtgcgcgg catcctcaac ggcacctgca attacattct cacccagatg
      361 gaatccaccg gccgtccctt cgaagacgta ctcaaggacg cgcaggcgct cggctatgcc
      421 gaggccgatc ccaccttgga cgtcggcggc ggcgacactg cccataagct cgcgctcctg
      481 gcgggactgg ccttcggcgt ggcgcccgat ctcgatacga tggcggtgca aggcatcact
      541 catatcatgc cagaagacat cgcctttgcg cgcgagttcg gtttccgcat caagcttctg
      601 ggaatcgcga aaaagacgcc gcagggaatc gatcagcgcg tacagccggc catggtgcgc
      661 gccggtacgc cgctcggcga cgtggacggc gtttataacg ccgtgtcggc cgacaccggc
      721 gcggcagggt acttcttttt cgaagggcgc ggagccggcg aagcgccgac cgccaacgct
      781 gtcatcgccg atatcgtcga catcgcacgc ggcaacatcg gacctgtgtt cggaatacag
      841 gcgcgttcgc tgataccgct aacacctgcg ccgggcggta gcgcgtcgtc cgcattttat
      901 ctacgcttcc aggtgctgga tgttccggga gtgttggcgg agatcgcggg gcatctggcc
      961 aagtggcgcg tttcgatcga aagcatgatc caacgcggac gcgccccggg ggaggccgta
     1021 tcgattgtca tgatcacgca cgaaaccacg caagccgcgg tcgattgcgc attgaagact
     1081 attgctgcgt ccgacaaaat catttcgcct ccctgcatga ttcccatgga agcgaaatag
     1141 ctccgctccg ccaggatcag gagactttat ggccggccgt ctgctagacc gcgcgtttgc
     1201 gctggaaacc gtgcgtatca ccgaagcggc ggccgtcgcg gcttcggaat tcgtcggccg
     1261 cggcgacgaa catgccgccg accgcgccac cgtcgaagcc atgcgcaaga ctttcaacta
     1321 tcttccgatc aacggcacgg tggtgatcgg cgaaggcgaa cgcgacgaag cgccgatgct
     1381 ctatatcggc gaaaaagtcg ggtgcggcgg cgtggacgtc gatatcgcgc tcgatccgct
     1441 tgaaggcacc gcattgaccg ccaaggcgct gcccaatgca ttggcgatcg tggcgatggc
     1501 ggagccgggg acgctgctca acgccccgga cacctacatg gacaagatcg cgatcggcgg
     1561 cggctatccc gaaggcacca tcgacctcga tgccgaaccg gcggagaacg tcctcgcgct
     1621 ggccaaggcc aagaacgtgc ctccgcgcga gatcaacgtc tgcatcctgg atcgcccgcg
     1681 ccacgaaacg cacatcgcct cggtgcgcag cgccggcggt tccgtccgcc tcattaccga
     1741 cggcgacgtt gcgggcatca tcgcgaccag cgatccggcc accggcatcg atatgtgttt
     1801 gggccggggc ggtgcgccgg aaggcgtgct ggccgccgcg gcgctgcgct gcgtgggcgg
     1861 ccaaatgcag gcacggctgt ttttcaagtc cgacgacgaa aagaagcgcg cgaccaagct
     1921 ggggatccgg gatttcgacc gcaagtacac gctgatggac cttgccagcg gcgacgtgat
     1981 ctttgccgca accggcgtga ccgacggggc gatgctgcaa ggcgtctacc gcacgccgga
     2041 gttcgtcttc acgcacagcg tggtgatgcg ttcggcttcg ggcacggtgc gctggataaa
     2101 agcgcgccac cgccgccatg agcattgacg cgcacagggc aggcggtcgg acgccttcgc
     2161 aggagggtgc acgaagcctg ttgggggttt cgctgtcttt ttcgggacgg cgctggatcg
     2221 tcccgcagag cgacgaggac gccattcgcg ccatggcgga gcatgatatt ccgcttgcgc
     2281 tggcgcgcgt gctggcggcg cgcggcgttt cggcaaacga ggcggcggac tatcttaacc
     2341 cgaccctgaa gcggctcctt cccgaacctt tgacgctcaa ggacatggac aaggccgtcg
     2401 cccgcgtcgc ggcggccgtc gcgaacggcg aacgcatagc ggtgttcggc gactacgacg
     2461 tggacggctc ttgttcggcg gcgttgttgc acgacttttt ggcctcggtc agtgcgccgc
     2521 cgcgcctcta tatccccgac cggatgagcg aaggctatgg gcccaatgca gcggcgttgc
     2581 tgcggctgaa ggccgaagga attggtctcg tcgttaccgt cgattgcggg gcgggcgccg
     2641 cggacgcatt gacggtggcg cgcgaagcgg gcctcgacgt gatcgtgctg gatcatcacg
     2701 ccgtcgagcg cgaaccgcct tgcgtcgccc acgtcaatcc caaccggcac ggcgatacct
     2761 ccggcctcgg gcatgtctgc gccgcgggga tcgtgttcct gttcctggtc gccttgaacc
     2821 gtgcgctgcg cgaggcggga cgatatgccc gaaaggccga accggatctg cgcgacgcgc
     2881 tcgatctcgt cggcctggcg accgtatgcg atgtcgtgcc gctcatcggg gtcaaccgcg
     2941 ccttcgtgca gacgggactg gccaggctcg gaaaactgga gcggccgggc cttgccgccc
     3001 tggcggccat tgcgcgcgtt gccccgccgt tcacgccgta tcacttggga tttatcctcg
     3061 gcccacggat caacgcgggc ggacgcgtcg gacgttgcag cctcggcgcc gacctgctga
     3121 cggccaaggg gcccgaggcc gaggagttcg ccgcggccct cgacagccac aaccgcgagc
     3181 gccaggccat cgagaagctc attttggacg aggcgatggc gcaagcggcg gcgcaggaca
     3241 atcagccgtt cctgttcttg gcaggcgatg gctggcaccc gggcgtcgtc gggatcgtgg
     3301 ccggcaggct caaggagcgc ttcggcaagc cggcgttcgt ggcggggttt gagggcggcc
     3361 tggggcgcgg ctcggcgcgt tcgatcgcgg gcgtggacat cggggcggtg gttcgcgccg
     3421 cacgcgaagc aggcacgctc gatgccgggg gcggacacgc aatggcggcc ggattttcgc
     3481 tgcgccccga acagaccgat ccgttccgag ccttcctggc ggccgagttc gcgaaattca
     3541 gcgacacatc ggcgctggtt gcgaccctga cgcttgaggc ggtggtttcg cccgccggcg
     3601 ccacgcctgc gctggtgcac gacatcggcc gggcaggtcc cttcggcgcc ggcaatcctg
     3661 agccgctttt ggccgttccg gacgccgagg tggccttcgc cgacattgtc ggcgccgacc
     3721 atgtacggct ccggctgacc ggcggcgacg gggcgcggct cgatgccatc gctttccggg
     3781 ctgcggacac ggatttgggc cgcgccctgc tgggcgcccg cggcaaacgg atccacgcgg
     3841 ccgggcggtt gcgcgccgac gaatggaacg ggcgcacccg ggtccagctt cacctcgacg
     3901 atgccgcgcc cgcggacgcc tgacggcgct tgccaaggcc gtttcagggc cttatagccg
     3961 cgtccttccg ggccgcgcgc aaggcgcgac tgggcgtggg cccttcgtct atcggttagg
     4021 actcaagatt ttcaatcttg caagacgggt tcgactcccg tagggcccac cagccttcgc
     4081 tcgcgaggcg acagaagact ctctgccgtg gcggtcgcca tgagcgccgg cggatggttt
     4141 attgccatat aaatcatgcg ggcttttcca ccgccgtcaa agcgcgaacg ctccattccc
     4201 gcaaagatcg catccgatgg cgcgacgccg gcgaggggcc gtgcgggcgc atcccctaaa
     4261 acaggcacct aaattgctac aatacgaaag aagtctttct acggcgccgg gctccgccgc
     4321 gcagcggaat tcgccgacga aacagcgtta atttccgcat tcgaatttat aaaggctact
     4381 agatttcgca acggaaactt tgtccgctcg gcgggttatt cggatgttta cccgctagtg
     4441 ccaaagtaga cgcttccaaa ggaagcgatt gaacggccat atgacctggt cttcatcaca
     4501 aaaacaaaag tctgtgggga tgaatctaac actcgacaga ttgcgccttg tgttcgtcga
     4561 cggcgaacaa gaaaaggcgt ttgtgcgcga ggcgttgatc gcgtccatcg gatttatccg
     4621 ggcctattta atcgccggca ccgggctgta catggtgttc ggcctgctgg acgctttggt
     4681 cggcgctcag tcggtccagg cgctatggat cgttcgctac gcggttgttt gcccgctctt
     4741 gatcggcgtg ttcgtcctta ctttttttcc ggtcttcgcg cgtataggtc agtacgcgct
     4801 tggcagcacg atgctcgtgt ccgggttcgg gatcattgtg atgacggcaa ttatgccgcc
     4861 gccgttcaac gccatgtatt acgccggcct cgtcatggtc gtgatctatt gctccagctt
     4921 gatccgactg caattcaatc tctccgcggc gatctctact tttcttgtcg tctgctatca
     4981 aatcgtggcg atctggatca atccggtgcc gctgacgacc ttcatcagca acgatttctt
     5041 tctgatcatg gctacggccg tcgggttgtt ttccggctac attcaagaat tgtatatcag
     5101 acgatcgtat atcagccaga aggtcatcga agccaagaac gaagcgctca acgttctgct
     5161 tgtcgaagcg gataaggcga acaagtcgaa gagcgaattc ctcgccaaca tgagtcacga
     5221 actgcgtacg ccgctgaacg ccattatcgg cttctccgac atattgaaga agcaattgtt
     5281 cgggccgctt ggaagcaata ggtaccagga gtacgtcaag gatatcaacg acagcggcgc
     5341 gcatctgctc gatatcatca acgacatcct ggatctggcc aaggccgagg ccggcaagct
     5401 cgaactgcaa cccggcgaac acgatctggc cgaatgcctc gacgactgca ttcgcatgtg
     5461 ccgcgcgcgc gccgagagtt gcggcgtgcg attgacattg ggcgaagtcg cctctccggt
     5521 tcttgctttt gtcgaccggc gtctgatttt ccaggcgctg ctcaatctcg cgaccaacgg
     5581 catcaagttc acgcctgccg gcggaacggt cacattgagc atggtctgcc cggaacagcg
     5641 tggcgtgga
//
LOCUS       DASWPU010000214         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_87113,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000214 DASWPU010000000
VERSION     DASWPU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87113"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VGC27_04670"
     CDS             complement(<1..296)
                     /locus_tag="VGC27_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0281899.1"
                     /translation="MDRKVEIEKRAVLLDGFFKVEEVYVSYEKFDGTMGPPVRRLNLA
                     RGDAVAAVLFNKARRRVILVNQFRYAALSRGSGWMTEIVAGLVDAGEAPEQAVRR"
     gene            complement(479..1390)
                     /locus_tag="VGC27_04675"
     CDS             complement(479..1390)
                     /locus_tag="VGC27_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281900.1"
                     /translation="MNKIPVTQTVAESYRFTFGGLSTVIGLIWLPIVILTVGGYFTLV
                     PYFSEMADALENGDVNRQGALMLRQLAFEIAMIVLMAMIAVAIVREILSPVQRPAFLR
                     FALGPAEFRAVGGFIGLFMLLIVFTLILKIIAPVIEYAAQAVLPAAGAAAGSKGAGGI
                     GALADVIGICVLIYLMVRLGFLMVPASVTEGRFGIERSWQLSKGNFWRILAVMVMTLL
                     PVILVAGVIDLAILGPDFLNPHLELARDPAAQMRRAAEQMRQMSVHLPLMMGVGFLLA
                     PFTYGMIFAPAAFAYRALTAKDETPQA"
     gene            complement(1387..>2210)
                     /locus_tag="VGC27_04680"
     CDS             complement(1387..>2210)
                     /locus_tag="VGC27_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281901.1"
                     /translation="GLVYLAVPHEWFAGDFSHFKPEQLVSLPLNLARLGIIVLSLVFG
                     SMIAVGLMQHALGLKKSTTFIYFSLGAPVWRMLGAILLAVVILVALGIVGGVLVALGA
                     QFGMPLIPHPYSIVALVALCFVVACFLVYAAVRLFFFLPAVVVAENRIGIGRAWSLGG
                     GNFWRIVFVWLLIAVPVGFIAAVALQMTILPVIFMEAMKLKQEPGPEEIFGFLHSLIP
                     LLPVILTITILASIATRGAMMGAIGSAYNAITAPPPDAAKPAEGAAAAAEGAANA"
BASE COUNT          457 a          736 c          675 g          342 t
ORIGIN      
        1 cggcgtacgg cctgttccgg cgcttccccg gcatcgacca gcccggcaac aatctctgtc
       61 atccagccgc tgccccgcga cagcgccgcg tagcggaact ggttcaccag gatcacgcgc
      121 cgccgcgctt tgttgaacag aaccgcagcc accgcgtcgc cgcgtgcgag attcagccgc
      181 cgcaccggcg ggcccatcgt tccgtcgaac ttttcgtagg aaacgtaaac ctcttcgact
      241 ttgaaaaacc cgtccaggag cacggcgcgc ttctcgattt cgactttacg atccatggcg
      301 gctctctatt tccccgctcc gtcgataccc gcttgcgccg gcaggcaccc ggcggcgatg
      361 gcgaacgtgc ttccggcaag aacgccgaga agaatcttat tcatgtccgg ggtccgtcgg
      421 tttggcatga aacacggcgg agcctaggcc aaaccttcgc ccgaccgcca cctggcattc
      481 aggcttgcgg cgtctcgtcc ttggcggtca gcgcgcggta ggcgaaggcc gccggcgcga
      541 agatcatgcc gtaggtgaac ggcgccagca ggaagccgac ccccatcatc agcggcagat
      601 gcaccgacat ctggcgcatc tgttcggccg cacggcgcat ctgcgccgcc gggtcgcgcg
      661 ccagctccag atgcggattc aggaaatccg gtcccagaat cgccaggtcg atgaccccgg
      721 cgacgagtat caccggcagc agcgtcatca ccatcaccgc caggatgcgc cagaaattgc
      781 ctttgctgag ttgccagctt cgttcgatgc cgaagcggcc ctccgtcacg gaagcgggca
      841 ccatcaggaa cccgaggcgc accatgaggt agatcaagac gcaaataccg atgacgtccg
      901 ccagcgcccc tatgcccccc gcgcctttcg accctgccgc cgccccggcg gcgggcagaa
      961 ccgcttgggc ggcgtattcg atgaccgggg cgataatttt caaaatcagc gtgaagacga
     1021 tcaacagcat gaacaggccg atgaatccgc cgaccgcgcg gaattcggcc ggccccagtg
     1081 cgaagcgaag gaacgccggg cgctgcaccg ggctcaggat ttcgcgcacg atcgccacgg
     1141 ctatcatcgc catgagcaca atcatggcga tctcgaaggc cagttggcgc agcatcagcg
     1201 ctccctgccg gttcacatcg ccgttttcga gcgcgtccgc catttcggaa aaatagggga
     1261 ccagcgtgaa atagccgccg accgtcagaa tgacgatcgg cagccagatc agcccgatga
     1321 ccgtgctcaa accgccgaaa gtgaagcgat acgattccgc gacggtttgc gtcaccggga
     1381 ttttgttcat gcgttcgcgg ctccttccgc ggctgcggcg gcgccctctg ccggcttggc
     1441 ggcgtccggc ggcggcgcgg tgatggcgtt ataggcgctg ccgatggcgc ccatcatcgc
     1501 ccctctcgtg gcgatgcttg ccaggatcgt aatcgtgagg atgaccggca gcaacgggat
     1561 caagctgtgc aggaagccga aaatttcctc cggccccggt tcctgcttca gcttcatggc
     1621 ctccatgaag atcaccggca ggatggtcat ttgcagcgcg acggcggcga taaacccgac
     1681 cggtacggcg atcagcagcc agacgaagac gatgcgccag aaattgccgc cgccgagcga
     1741 ccaggcgcgg ccgatcccga tccggttttc cgccaccacc acggccggca ggaagaagaa
     1801 cagccggacc gccgcataaa cgaggaagca agcgacgacg aagcaaagtg cgaccaaggc
     1861 gacgatcgaa tagggatgcg ggatcagcgg catgccgaat tgtgccccga gcgcgaccag
     1921 cacgccgccg acgatgccga gcgccaccag gatcacgacc gccagcagga ttgcgccgag
     1981 catccgccac accggcgcgc ccagcgagaa gtagatgaag gtcgtcgatt tcttcagccc
     2041 cagcgcgtgc tgcatgaggc cgaccgcgat catcgagccg aagaccagcg aaaggacgat
     2101 aatgccgagg cgcgccagat tgagcggcaa cgaaacaagc tgctcgggtt taaagtgcga
     2161 aaagtcgccg gcgaaccatt catgcggcac ggcgagatag accagcccgg
//
LOCUS       DASWPU010000215         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_75118,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000215 DASWPU010000000
VERSION     DASWPU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75118"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..850)
                     /locus_tag="VGC27_04685"
     CDS             complement(227..850)
                     /locus_tag="VGC27_04685"
                     /inference="COORDINATES: protein motif:HMM:NF017973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281902.1"
                     /translation="MKPENPSEPFKRAVCTAVRALAGEPELEVTYSAEPPQLRGQKAR
                     LPLPSRHLSPHEAAVIRGAGDAYALKLAYHKGNIHAQFRPRAPESAALFEAAEQARVE
                     AIGALAMKGVAENLAASLEAKCSARGLATVRDKAQAPLADVLGMIVREKLTGAPPPET
                     ARRAVDLWRYFIETRAGKDLDKLATALRDQKAFAKLTRTVLKDLDFG"
     gene            complement(936..1931)
                     /gene="cobS"
                     /locus_tag="VGC27_04690"
     CDS             complement(936..1931)
                     /gene="cobS"
                     /locus_tag="VGC27_04690"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HEY0281903.1"
                     /translation="MTSETQGLDPSLTPDTQIDVKAAFGIEARMRVPAFAKPNEYVPD
                     VDNAYRFDRDTTLAILAGFAFNRRVMIQGYHGTGKSTHIEQVAARLNWPCVRINLDSH
                     ISRIDLIGKDAIVLREGQQVTEFREGLLPWALQHPVALVFDEYDAGRPDVMFVIQRVL
                     EVQGKLTLLDQNRVIRPHPAFRLSSTTNTIGLGDTTGLYHGTQQINQGQMDRWSIVAT
                     LNYLTHDSEVEIVLAKVPSYASTPEKKAAIASMVRVADMTRNAFVNGDLSTVMSPRTV
                     ITWAENADIFGDVGFAFRLTFLNKCDELERASVAEFYQRCFGEELPESAAKVLVA"
     gene            complement(2007..2603)
                     /locus_tag="VGC27_04695"
     CDS             complement(2007..2603)
                     /locus_tag="VGC27_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY0281904.1"
                     /translation="MAKTRYRPRLGHDIRIKPDGKVPPPVKVRPCAEPSCREEGAYRV
                     PKSHEHLSTYLWFCLAHARAHNEKWDYFAGMSMAEIERFRTEAIYGHRPTWPLGKRAA
                     GFSFGRSGAQIEDGYEVFAEEKTAPRRPERVLPRLQQQAFDVLNLDTSATLNEIKARY
                     KELVKRFHPDANGGDRGAEERLKQVIKAYGVLRASGLT"
BASE COUNT          414 a          853 c          903 g          572 t
ORIGIN      
        1 gccagggctt gacgccttcc tcgggatcga tcgtgtcgga agaatcgtcg ctgccgtcgg
       61 cgcgcatttc ggcgctggcg tctttgcttt cgccttcggc ttgctccatg tcggcgtcgg
      121 cggaagcggt ttcgccgctg gcgtcctgcg cttcgctgct ctcccgctcg gaggggcttt
      181 cgttttcctc gccgccttcc tccttttcgg agtcctggcc gtcgtcctag ccgaaatcca
      241 aatccttgag caccgtgcgc gtcagtttgg cgaacgcctt ctggtcgcgc agcgccgttg
      301 cgagcttgtc gaggtccttg cccgcacggg tttcgatgaa atagcgccac agatcgacgg
      361 cgcgccgcgc cgtttcgggc ggcggcgccc ctgtcagttt ctcgcgcacg atcatgccca
      421 gcacgtcggc gagcggggct tgggccttgt cgcgcaccgt ggccagaccg cgcgcgctgc
      481 atttggcttc caggctcgcc gccaggtttt ccgccacgcc cttcatcgcc agcgcgccga
      541 tggcttccac gcgggcctgc tcggccgctt cgaacaaggc cgcgctctcc ggcgcgcgcg
      601 ggcggaactg cgcgtggata ttgcccttat gataggcgag cttgagggca taggcgtcgc
      661 ccgcgccgcg tatcacggcg gcttcgtgcg gcgaaagatg gcgcgagggc agcggcaggc
      721 gcgctttttg tcctcgcagt tgcggcggct cggcgctgta ggtgacttca agctccggct
      781 cgccggccag cgcccgcacc gccgtacaga cggcgcgctt gaacggctcg gaggggtttt
      841 cgggtttcat agaaggggcg aatagcgagt ggcgaatggc gaatagaatc ctctattccc
      901 tactcgccat tcgctattcc ctgttcgctt cttcttcacg ccaccagcac cttggccgca
      961 ctctccggca gctcctcgcc gaagcagcgc tgatagaact cggccacgct ggcgcgttcc
     1021 agttcatcgc acttgttgag gaaggtcagc cggaaggcga agccgacgtc gccgaagata
     1081 tcggcgttct cggcccaggt gatgacggtg cgcgggctca tcacggtgga gaggtcgccg
     1141 ttgacgaacg cgttgcgggt catgtcggcg acgcggacca tcgacgcaat cgccgctttc
     1201 ttctccggtg tcgaagcata ggacggcact ttcgccagca cgatctccac ttcgctgtcg
     1261 tgggtgaggt aattgagcgt cgccacgatg ctccagcggt ccatttggcc ctgattgatc
     1321 tgctgagtgc cgtgatagag gccggtggtg tcgcccaggc cgatggtgtt ggtggtcgag
     1381 gacaggcgga acgccggatg cggccggatc acgcggttct ggtcgagcag ggtcagcttg
     1441 ccttggactt ccagaacgcg ctggatgacg aacatcacat cgggccggcc tgcgtcgtat
     1501 tcgtcgaaca ccagcgccac cggatgctgc aacgcccagg gcagcagccc ttcgcggaac
     1561 tccgtcacct gctggccttc gcgcagcacg atggcgtcct tgccgattag atcgatgcgg
     1621 ctgatgtggc tgtcgaggtt gatgcgcacg cacggccagt tgaggcgcgc cgcgacctgt
     1681 tcgatgtggg tcgatttgcc ggtgccgtga tagccctgga tcatgacccg gcggttgaag
     1741 gcgaagccgg ccaggatcgc cagcgtggtg tcgcggtcga agcggtaggc gttgtcgaca
     1801 tcggggacat attcgttcgg cttggcgaac gcgggcaccc tcatcctcgc ctcgatgccg
     1861 aaggcggcct tcacgtcgat ttgcgtatcg ggcgtcagcg aaggatcgag gccctgcgtt
     1921 tcactggtca tgtctgttgg tccggctgtg acattcgcgg gcttccgccg cgggtgagga
     1981 ggagggcgcc agagcccttc ttagtctcag gtcagccccg aggcgcgtaa tacgccatag
     2041 gctttaataa cctgcttcaa ccgctcctcg gcgccccgat cgccgccatt cgcgtcggga
     2101 tgaaaccgct tcacgagttc cttataccgc gccttgattt cgttcaaggt ggccgatgtg
     2161 tcgaggttca gcacgtcgaa agcctgttgt tgcaggcggg gcaagacccg ttcgggccgg
     2221 cgtggagcgg ttttctcctc tgcaaaaacc tcatatccgt cctcgatttg ggcgccgctg
     2281 cggccgaacg agaacccggc ggcgcgcttg cccagcggcc aggtcggccg atggccgtaa
     2341 atggcctcgg tgcggaagcg ttcgatctcc gccatactca tgccggcgaa atagtcccat
     2401 ttctcgttgt gggcgcgggc gtgggcgaga cagaaccaaa ggtaggtgga aagatgttcg
     2461 tggcttttgg gcacccgata ggcgccttct tcgcggcagc tcggctcggc gcaagggcgc
     2521 actttcaccg gcggcggtac cttgccgtcc ggcttgatcc tgatgtcgtg ccccagtcgg
     2581 gggcggtagc gcgtcttggc catggctgaa ttatgggatc gatccgtgaa aaaacaagaa
     2641 aacgaattac ttgacagagc gctgcgcgcg cgcgacgtcc ttagagatgc cctttcgcct
     2701 tcatcccgag gggcgcgaag cgccttcttg atgggcgagg gc
//
LOCUS       DASWPU010000216         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_26050,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000216 DASWPU010000000
VERSION     DASWPU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26050"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /gene="dnaN"
                     /locus_tag="VGC27_04700"
     CDS             complement(<1..668)
                     /gene="dnaN"
                     /locus_tag="VGC27_04700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY0281905.1"
                     /translation="MKALSHVQSVVERRNTIPILSNVMIEAAKGQLKLTATDLDIEIV
                     EAIPSDVLRNGAATAPAHMLYDIVRKLPEGAQASLETSGDAGQLVLRSGRSRFTLQAL
                     PASDFPDLTSGDFPHRFSLPAVELKRLIENTQFAISTEETRYYLNGIYLHTVDIGGTP
                     MLRAVATDGHRLARVELPLPKGAGDMPGVIVPRKTVGEVQRLIEDNEAEVAIELSSGK
                     IRFT"
     gene            986..1882
                     /gene="ppk2"
                     /locus_tag="VGC27_04705"
     CDS             986..1882
                     /gene="ppk2"
                     /locus_tag="VGC27_04705"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY0281906.1"
                     /translation="MTKHKKAKLRKIRNEVRSNAKEKARVEGNWHILGDAKPPKPPGK
                     NYSYIDELTRLQFELLKMQEWVRLQGLKVVVIFEGRDAAGKGGVIKRISEVLNPRFCR
                     VVALATPTEREVGQWYFQRYVEQLPARGEIVLFDRSWYNRAGVEHVMGFCTRDEYKEF
                     LRACPLFEEMLVRSGIYLIKYWFSVDDEEQERRFRDRMANPIKRWKLSRMDVESRERW
                     VEYSRAKDMMLKHTDKKLTPWHIVDAHNKKRARLNCIAHLLHQIPYKNLRPVQFELPP
                     RQPDTGYIRPKKSSQRWIPERY"
     gene            2098..2418
                     /locus_tag="VGC27_04710"
     CDS             2098..2418
                     /locus_tag="VGC27_04710"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY0281907.1"
                     /translation="MDKDRVKGSADQAIGAVKETAGKLTGDAKLKAEGKAGKLKGKVD
                     SAVGGAKDAVRHATRKLDRALCSDAVANAGSAVRDAVRSRPAGALALAAGIGALLGAL
                     IARA"
BASE COUNT          497 a          759 c          793 g          437 t
ORIGIN      
        1 atggtgaagc ggatcttgcc gctcgacagc tcgatggcga cctcggcctc gttgtcctcg
       61 atcagccgct gcacctcgcc gaccgtcttg cgcggcacga tcacgccggg catgtcgccc
      121 gcacccttag gcagcggcag ctcgacgcgc gccaggcgat ggccgtcggt cgcaaccgcc
      181 cgaagcatcg gcgtcccgcc gatgtcgacg gtgtggaggt agataccgtt caggtaatac
      241 cgcgtctcct cggtcgaaat cgcgaactgg gtgttctcga tcaggcgctt gagctcgacc
      301 gccggcagcg aaaagcggtg cgggaaatcg cccgaggtga gatcgggaaa gtccgaggcc
      361 gggagcgcct ggagggtgaa gcgcgagcgg ccggagcgca acacgagctg cccggcgtcg
      421 ccgctcgtct cgaggctcgc ctgcgcgcct tccgggagct tgcgcacgat gtcgtagagc
      481 atgtgcgccg gtgcggtcgc ggcaccgttg cgcagcacgt ccgacgggat cgcctcgacg
      541 atctcgatat cgagatcggt ggcggtgagt ttcaactgcc ccttggcagc ctcgatcatc
      601 acattggaga ggatcggaat ggtgttgcgc cgttcgacga cactttgcac atggctcagc
      661 gccttcaaaa aagcgccccg ttcgacgttg attttcatgg ctctatcgac tgccgtgcgg
      721 ttgttggaaa agccccttcc ggttccctct cggggcgacc ggaaaccctt gatttccttg
      781 ggcttttaaa agccaaagac tcaagtgcga aattatatca ggcgcgtgac caaagggcca
      841 gctttggagg gggtccgaat ccttctcccc cacaggggaa gagggtagga tgagcggggc
      901 agcgaagccc gcctgttcat cctatcttgg agaatttgcg gcagcatgcc accagtgcca
      961 atgtggcgct gacggcgcaa aggccatgac caagcacaag aaagcgaagc tccggaaaat
     1021 ccgcaacgag gtgcgcagca acgccaagga gaaggcgcgg gttgagggga actggcacat
     1081 cctcggcgac gccaaaccgc ccaagccgcc gggcaagaac tactcctaca tcgacgaact
     1141 cacccgcctg caattcgagc tgctcaagat gcaggaatgg gtacggctcc agggcctcaa
     1201 ggtggtggtg atcttcgagg ggcgcgacgc ggccggcaag ggcggcgtga tcaagcgcat
     1261 ctccgaagtg ctcaacccgc gcttctgccg cgtcgtggcg ctggcgacgc ccaccgaacg
     1321 cgaagtgggg caatggtatt tccagcgcta cgtggagcag cttccggcgc gcggcgagat
     1381 cgtgctgttc gaccgctctt ggtacaaccg cgccggggtc gagcatgtca tgggcttctg
     1441 cacccgcgac gaatacaagg aatttctgcg cgcctgcccg ctgttcgagg agatgctggt
     1501 gcgctctggc atctacctga tcaaatactg gttctcggtc gacgacgaag agcaggagcg
     1561 ccgcttccgc gaccgcatgg cgaatccgat caagcgctgg aagctgtcac gcatggacgt
     1621 cgaatcccgc gagcgctggg tggaatattc gcgcgccaag gacatgatgc tgaagcacac
     1681 cgacaagaag ctgacgccct ggcacatcgt cgatgcccac aacaagaagc gggcgcgcct
     1741 caactgcatc gcccacctgc tgcaccagat tccctacaag aatttgcgcc cggtccagtt
     1801 cgaactgccg ccgcgccagc ccgacaccgg ctatatccgc ccgaaaaaat ccagccagcg
     1861 ctggataccg gagagatatt gaagctctct ttcaccttcc cttgcggggg ccggtacggc
     1921 cctcggcgac aagcatttga tcgttgaacg cactcttttg tgccaaaacg ctgcaaactc
     1981 gcgttaaccg cgtcgccttg tatgagtagt ttccgacgct gcccctggca atcacgcgtc
     2041 ggctacactg tgagttggtg tccgggtgtt atctcgccgg accaggagaa cggaacgatg
     2101 gataaggatc gcgtcaaggg atcggcggac caagcaatag gcgccgtcaa ggaaactgcc
     2161 ggcaagctta ccggagatgc caagctcaag gcggaaggca aggccggcaa gctgaagggc
     2221 aaggtggata gcgcggtcgg cggcgcgaaa gacgctgtca ggcacgcaac caggaaatta
     2281 gaccgcgcct tgtgctctga tgccgtggca aacgctggct cggccgtgcg cgacgccgtc
     2341 agatcgcggc cggccggcgc cctcgcgctg gcggccggca tcggcgccct gctgggcgca
     2401 ctgattgcgc gcgcctagac acaatggctg cgcgtgttga agacagcggg agttcgtcat
     2461 gctgaaatgg gcattcgttt ttctcg
//
LOCUS       DASWPU010000217         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_44844,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000217 DASWPU010000000
VERSION     DASWPU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44844"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VGC27_04715"
     CDS             <1..1126
                     /locus_tag="VGC27_04715"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0281908.1"
                     /translation="WLVYSMTHSPFLLGVTSFAAQVTVFFVAPFGGMLADRVDRRKLL
                     LLTQSLAMLQAALLAGLTLAGVVQVWEIIALAFGLGLINAFDVPTRQAMTLDLVGGPE
                     DLRHAIALNSMMFNLARVIGPSLAGAVIAVVGEGVCFALNTVSFAAVLWSLILMRLPV
                     RPTRVHATALHEIAEGYRFSLSTPQIRTSLMLVAVSSCFGAAYLSLMPAFAHDHLHGG
                     AGSYGLLMAGVGIGALLGAYGLSLIRERWLTLTPVAAALCFGLGLIAFSHSHMLWLSV
                     LLLLPTGASLMLLGGTTNTLIQILAGERYRGRVISHYTQSFIGMMPWGSLLLGWAAAQ
                     FGVVEAVTFGGLVVMASGVLAWFQRRGQEWRLDKLSSSRD"
     gene            complement(1457..1808)
                     /gene="ssrA"
                     /locus_tag="VGC27_04720"
     tmRNA           complement(1457..1808)
                     /gene="ssrA"
                     /locus_tag="VGC27_04720"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            1841..2122
                     /locus_tag="VGC27_04725"
     CDS             1841..2122
                     /locus_tag="VGC27_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY0281909.1"
                     /translation="MKIEFDPAKSEKNARERGLPFTMVENFDWGTSYSEEDTRFPYPE
                     RRWITTGFLGGRLHVVCHTPIEGGTRVISFRKANSREVRKYEEKKAADE"
     gene            2094..2363
                     /locus_tag="VGC27_04730"
     CDS             2094..2363
                     /locus_tag="VGC27_04730"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0281910.1"
                     /translation="MKKRKPLTNKDGEVRELTAEDFAAMRPTAEVLPELVALWRKRGQ
                     RGPQKAPTKQQVTLRLDRDVVERFRATGRGWQGRINEALRKARVG"
     gene            2416..3210
                     /locus_tag="VGC27_04735"
     CDS             2416..3210
                     /locus_tag="VGC27_04735"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY0281911.1"
                     /translation="MRQYLDLMDRVLKTGVEKRDRTGTGTLSIFGHQMRFDLGEGFPL
                     VTTKKLHLKSIIYELLWFLRGDTNVRWLREHGVSIWDEWADANGELGPIYGHQWRAWP
                     RGNSGTIDQIAAVVADIKRNPDSRRLIVTAWNPADLPKMALPPCHCLFQFYVAEGKLS
                     CQLYQRSADVFLGVPFNIASYALLTLMMAQVTGLKPGEFVHTFGDAHLYLNHLEQARE
                     QLSRPPYPLPAMRIDPKVTDIFSFKYEDFELENYQAHPHIKANVAV"
     gene            complement(3239..3769)
                     /locus_tag="VGC27_04740"
     CDS             complement(3239..3769)
                     /locus_tag="VGC27_04740"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEY0281912.1"
                     /translation="MKKFLVSAAVLGLALGLAAAQAAPNDHNNRTAAENSRKGATNPG
                     NSMQRAPGGAIKPPQHQAPATKGPQSGRPSMMGGPQGKGRVDVTRFRGNVHAQKRFRS
                     GTYRAPRSYKYRRWTYGERLPAIYFARDYWLPNFVTFGLMAPPYGYGWVRFGPDALLV
                     DLRTGEVIRVVYGIFY"
BASE COUNT          682 a         1229 c         1296 g          807 t
ORIGIN      
        1 gtggctggtc tacagcatga cccattcgcc ctttctgctg ggcgtgacct cgttcgcggc
       61 gcaggtgacg gtgtttttcg tcgcaccgtt cggcggcatg ctggccgacc gggtggaccg
      121 gcgcaaattg ctgctgctga cgcagagcct ggcgatgctg caagccgcgc tgctggcggg
      181 gctgacgctg gccggggtgg tgcaggtgtg ggagatcatt gcgctcgcct tcgggcttgg
      241 cctgatcaac gccttcgacg tgccgacgcg ccaggccatg acgctcgacc tggtgggggg
      301 tcccgaggat ttgcgccacg ccatcgcgct caattcgatg atgttcaatc tggcgcgggt
      361 gatcgggcct tcgctggcgg gcgcggtgat cgcggtggtg ggcgagggcg tctgctttgc
      421 gctgaatacg gtgtccttcg ccgccgtgct gtggagcctc atcctgatgc gcctgccggt
      481 caggccgacg cgcgttcacg ctacggccct gcacgagatc gccgagggct accgcttttc
      541 gctgtccacg ccgcaaatcc gcacttcctt gatgctggtc gcggtctcgt cctgcttcgg
      601 cgccgcctat ctgtcgctga tgcccgcctt cgcgcacgac catctgcacg gtggtgccgg
      661 gagctacggc ttgctgatgg ctggcgtcgg catcggagca ttgctcggcg cctacggcct
      721 gtcgctgatc cgcgaacgct ggctgacgct gacgccggtg gcggccgcgc tttgcttcgg
      781 gcttggactg atcgcgtttt cgcactcaca catgttgtgg ctgagcgtgc tgctgctgtt
      841 gccgaccggc gccagcctga tgctgctggg cggcaccacc aacacgctga ttcagattct
      901 ggccggcgag cgctatcgcg gccgggtgat ctcgcactac acgcagagct tcatcggcat
      961 gatgccgtgg ggatcgctgc tcttgggatg ggccgcggcg cagttcggcg tcgtcgaggc
     1021 ggtgaccttc ggcggcttgg tcgtgatggc ctcaggcgta ctcgcctggt tccagcggcg
     1081 cgggcaggaa tggcgcctcg acaaactctc ttcgtcccgc gattgatttt ccgtcccctg
     1141 cctttttctg cccccgccct gcccaccgaa gctttagcgt aggtggggag cgggggaagt
     1201 gtccggcgta gccggacgaa gggggccgag cgtagcgagg aagtgggacg tgcttctgct
     1261 tgtggccggg cttgacccgg ccattgcccg gtgcacgtcc ctgcgaagcg cgcgcgagcg
     1321 cgttcgcatt cataactcgg gggtgccgaa gtctcggcga aatcagtggg gggtttctgt
     1381 tgcccggcac ccagcgaagc gcgcgcgagc gcgttcgcat tcataactat gggggtgccg
     1441 aagtctcggc gaaatcagtg gggggtttct gttgcccggc accccccgag ccgcgcttgt
     1501 ctaactagaa gttaggcagc gagggccatt tcattatcgt tggcacctat acgagtagcc
     1561 cgataacggc ggataccata ccgagcgaaa gtactgcctt tacacgtccg tcgatcctat
     1621 ttcgcccccg catcagcccg ccttcgccct tcaggcttcg gtgggcaatc acccttcggg
     1681 ggtgattggt ggaggcgccg ggtaccgccc ccgggtccgg gccgcttatt acgcaatcgt
     1741 ttatcgccat agtccgggca agcccgaacc tgtgtaatat aggtgttgcg ggttaagact
     1801 cctaggcctt gacagcaatt ctttttgtaa ctacaattaa atgaagatcg aattcgatcc
     1861 cgccaagagt gagaagaacg cgcgcgagcg cgggctaccg ttcacgatgg tcgaaaactt
     1921 cgactggggg acatcttatt cggaggaaga cacgcggttt ccctaccccg agcggcggtg
     1981 gatcacgacc gggtttcttg gcggtaggct gcatgtcgtc tgccatacac cgatcgaggg
     2041 tggaacccgc gtcatcagtt ttcgcaaggc caatagtcgt gaggttcgga aatatgaaga
     2101 aaagaaagcc gctgacgaat aaggacggcg aagtgcgcga gctgacggcc gaggatttcg
     2161 ccgccatgcg gccgaccgcc gaagttctgc cggaactggt ggcgttatgg cgcaagcgcg
     2221 gccagcgcgg gccgcaaaag gcgccgacca aacagcaggt gacgctgcgt ctcgaccgcg
     2281 acgtggtgga acgcttccgc gccactggtc gcggctggca gggccgtatc aacgaggcgc
     2341 ttcgcaaggc gcgggtggga tagactggcg cggcagagta gagtgcgcat ggaatcggcg
     2401 gacccgcgat aacgaatgcg ccaatatctc gacttgatgg accgcgtgct gaaaaccggc
     2461 gtggagaagc gcgaccgcac cggcacgggc acattatcga tcttcggcca ccagatgcgt
     2521 ttcgatctgg gcgaaggctt cccgctcgtc accaccaaga agctgcacct gaagtcgatc
     2581 atttacgagc tgctgtggtt cctgcgcggc gacaccaatg tgcgctggct gcgggagcac
     2641 ggcgtctcca tctgggacga gtgggcggat gccaacggcg agctgggtcc gatttacggc
     2701 catcagtggc gcgcctggcc gcgcggcaat tccggcacca tcgaccagat cgccgccgtg
     2761 gtcgccgata tcaagcgcaa tcccgattcg cgccggctga tcgttaccgc ctggaacccg
     2821 gccgacctgc ccaagatggc gctgccgccc tgtcattgcc tgttccagtt ctatgtcgct
     2881 gagggcaagc tctcctgcca gctttaccag cgctcggccg acgtcttcct cggcgtgccc
     2941 ttcaacatcg cctcctatgc gctgctcacc ttgatgatgg cgcaggtgac gggcctcaag
     3001 ccgggcgagt tcgtccacac cttcggcgat gcacatcttt atctcaacca cctggaacag
     3061 gcgcgcgaac aactttcgcg cccgccttat ccgctgcccg cgatgcgcat cgatcccaag
     3121 gtgaccgaca ttttcagctt caaatacgaa gatttcgaac tggaaaacta ccaggcccac
     3181 ccgcacatca aggcgaacgt ggcggtgtga gcgccgcgtt cgccttgggc gtgcagactt
     3241 agtagaagat gccgtaaacc acgcggatga cttcgccggt tctgaggtcc accagcagcg
     3301 catccggtcc gaaacgaacc cagccataac catagggcgg cgccatcagg ccgaaagtca
     3361 caaaatttgg cagccaataa tcgcgggcga agtagatggc cggaagccgt tcaccgtagg
     3421 tccagcggcg atacttgtag gagcgcggcg cgcgatatgt gccgctgcgg aagcgctttt
     3481 gcgcatggac gttgccgcgg aagcgggtga cgtccacacg acctttgccc tgcggaccac
     3541 ccatcatgct tgggcggccg ctctgcgggc ccttcgtggc tggggcctga tgctgaggcg
     3601 gcttgatcgc gccgccgggc gcgcgctgca tgctattgcc gggatttgtc gcgcccttgc
     3661 gggaattctc ggccgctgtc ctattgttgt ggtcgttcgg cgcggcttgc gctgcggcca
     3721 gcccgagagc gaggccgagc actgccgcgg acacgagaaa tttcttcatg gtctctcctt
     3781 ggggaagcag aaccgtccga tgcgattcac cggctccctt taagcgcgga gaatggtgcc
     3841 gtttccgccg cggcgcaatg cggaattgct ctgccgcata tggcttcgac ggcacgtcct
     3901 gcttgccaag cgtacaagtc tgctcgataa aagctcgata aaagattgcc ttacgtaagg
     3961 ccggcggccg gaaccgaggg aaacgctttg acaaatttct ctattcgcaa ggct
//
LOCUS       DASWPU010000218         4054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_69367,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000218 DASWPU010000000
VERSION     DASWPU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69367"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="VGC27_04745"
     CDS             complement(<1..1829)
                     /locus_tag="VGC27_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0281913.1"
                     /translation="MWGACCAMAAACLFCARPAYAQEAKYDFQIQQESLGAALSELSR
                     QAHVELLYPYQLARVRGNPIKGRYTVPEALDLLLEGTGFSGGLTSQGIVTISLRRKGC
                     NEEGKAMLPDSKSTVSAIALLAGILSAPACHAQAGGAPASSSDVSESIETVTVVGYRA
                     SLASSTNAKRDSVGFSESVFSEDIGKFPDTNIAESFNRIPGITIIREVNGEGLQVAIR
                     GLGVNFTKTLLNGAPVAMASTGRTDAQSTNREVDLNMFPAELFTQLTVSKSPTADMIE
                     GGAAGVVNMRSMRPFDNPGMHVTYAMQGQIGSVAGKLGEKGTLIVSNTWGQFGALVGV
                     TVNHSLVRTTGFEASNWTNPKLTAAQCGSSSGCNSTGGGIISIPATVPDTAASIAAGL
                     VPGQVIDKAWLLAHNPGLTIEQIDNGLLPRIARPSDNWGARDRANAVASLEYRPTDDL
                     HFFFDFIGGRLVNNERRTDMMLRLTGTPSYLPLNEQVDANNVMTSVDIVNNQFFLEDR
                     PYTEKEDFISFNPGMMWQLTDLLKVDLQANATRSHFFRDYPSLYAFTARTTSHISNPP
                     GAQFPTFTSPMDLNDPANWGWLPGGSGVHIQQETRYIYTNGARL"
     gene            complement(2099..3187)
                     /locus_tag="VGC27_04750"
     CDS             complement(2099..3187)
                     /locus_tag="VGC27_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0281914.1"
                     /translation="MHDHEKIVKFPDVSTVEAEAAEWIVRLDAGGPAEDYARFLEWQN
                     RSPLHREAALRLTALWSQFDALKEFAAPFPGAGERRQTWTRRLWNAGSEASWRKVASA
                     AALLVLLAGAGLFYTHKQPAPLVAIPYETALGSQKDISLPDGSTVVLNTNSRLDVVYS
                     KTRRDVYLHRGEAFFEIASSDKRPFTVHANAKQIHDIGTAFDVRLLRNAVEVTVTKGA
                     VELVNAARPDAPSKDKVERLGVVSAGQNAVFSRKLEYVKPLTDAEINRHLAWREGILV
                     YAGEPLARVVEDVSRYTNIRIELGDPKLRDIQVGGYFEISKIGSAFEALEKNFGIQAR
                     WRDTRHVRLYLANDPAAHRPLSSRSGDK"
     gene            complement(3180..3797)
                     /locus_tag="VGC27_04755"
     CDS             complement(3180..3797)
                     /locus_tag="VGC27_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0281915.1"
                     /translation="MSSILEAYLENEAAIKRFLKRFIKRSEDIDDLAQETFIRAFAAE
                     SAQTVKFPKAFLFRIARNLALNEREKCSNAKTDSLEDFADQAVLEDHSEAAPDDKMEF
                     RERVRLLAQAVASLPPQCSKVFLLRKVYGYSYKSIAYRLNISVSTAEKHVALGLLRCS
                     EYLRRQGYDIDTKSSHLTKAHRGGKAPNGVVTRLGVNPRKRVRDA"
BASE COUNT          802 a         1200 c         1176 g          876 t
ORIGIN      
        1 agacgcgcgc cgttggtgta gatgtagcgc gtctcctgct ggatgtggac gccggagcct
       61 cccggcagcc atccccagtt tgccggatcg ttgagatcca tgggcgaagt aaaggtcggg
      121 aactgcgctc ccggcggatt ggaaatatgc gaggtggttc tcgcagtgaa cgcatagagc
      181 gagggatagt cgcggaagaa gtggctgcgg gtggcattcg cctgcaggtc gaccttgaga
      241 agatcggtca gctgccacat catgcccgga ttgaagctga tgaagtcttc cttttccgtg
      301 taaggccggt cttcaaggaa aaactggttg ttcacgatgt cgacgctggt catgacattg
      361 ttggcgtcga cctgttcgtt caacggcagg taggatggcg tgccggtgag tctcaacatc
      421 atgtccgtgc ggcgttcgtt gttgacgagg cggccgccga tgaagtcgaa gaagaaatgc
      481 agatcgtcgg tgggccggta ttccaggctc gcgacggcgt ttgcccggtc gcgggcgccc
      541 caattgtcgg aaggacgggc aatacgcggc agaaggccgt tatcgatctg ctcgatcgtc
      601 agaccggggt tgtgcgccag cagccacgcc ttgtcgatca cttggccggg gacaagtccg
      661 gcggcaatcg acgcagcggt gtccggcacg gtggcgggaa tggagattat gccgccgccg
      721 gtggagttgc agccgctgct agaaccgcat tgcgcagccg tcagcttcgg gttcgtccag
      781 ttggaggctt caaagcccgt cgtcctgaca agactgtgat tgacggtcac gccgaccagg
      841 gcgccgaact gaccccaggt attgctaacg atcaacgtgc ccttttcgcc gagcttgccg
      901 gcgaccgaac ctatctggcc ctgcattgcg taagtgacgt gcatgcccgg gttgtcgaac
      961 gggcgcatgc tgcgcatgtt cactacgccg gcggcgccgc cttcgatcat atcggccgtc
     1021 ggcgatttgc tgacagtcaa ctgggtgaac agttcggccg ggaacatgtt caaatcgact
     1081 tcgcggttgg tgctttgcgc gtcggtgcgg ccggtggagg ccatggcgac cggagcgccg
     1141 ttgagcaggg tcttggtgaa attcacgcca aggccgcgga tcgcgacttg cagaccctcg
     1201 ccgttcactt cacggatgat cgtgatgccg ggaatgcggt tgaaggattc ggcgatgttg
     1261 gtgtcaggga atttgccgat atcctctgag aaaaccgatt cgctgaaacc tacggagtct
     1321 cgcttggcgt tcgtcgagct cgccaaactc gcgcggtaac cgaccaccgt gacggtttcg
     1381 atgctttccg agacatccga gctgcttgcg ggcgctcctc cagcctgcgc gtggcaggcg
     1441 ggcgcgctca gaataccggc aagcaatgcg attgcggaca ctgttgattt gctgtctggc
     1501 aacatggctt tcccctcttc gttacagcct ttgcgccgca aggagatcgt cacgatccct
     1561 tgcgaggtga ggccgccgga aaagcccgtt ccttcgagca gcaaatcgag cgcttctgga
     1621 actgtgtacc gaccttttat tgggtttccc ctgacccggg ccaattgata cggatagagc
     1681 agttcgacgt gagcctgccg agacaattcg gataaagctg cgcccaacga ttcctgttga
     1741 atttggaaat catacttcgc ttcctgggca tacgcaggtc gggcgcaaaa cagacacgcc
     1801 gccgccattg cgcagcaagc gccccacacc gccaaacgtt tcctctctcc cgtcactatt
     1861 ggtctcggtg atcagcacgt gtgtctggct ttcgcaccgg ccttgtagct cgtaagaccg
     1921 gcaagacgcg aaaaccctcc agaagattac cagagatttt tcgcaacgcg tgcggcggat
     1981 acgcgtacgc gtgatgcctt cacctgatgc aaacgtcctc tcggcaatgg ctgccagagc
     2041 acacgccgcg atgcgcatcg cccagtactc gcgccatggt tgccgatagt ggagtccgtc
     2101 acttatcgcc ggaccgggag gataatggcc gatgtgccgc aggatcgttg gccaaataca
     2161 gccggacatg cctggtgtcg cgccagcgcg cctgaatacc gaagttcttt tctaaggctt
     2221 cgaatgcgga cccgatcttg ctaatctcga aatatccgcc cacctggatg tctctcagct
     2281 tggggtctcc aagttcgatc cggatgttcg tgtagcggct cacatcttcc acaacccgcg
     2341 ccagcggctc tcccgcgtac accaaaatgc cttcccgcca agcgagatga cgattgattt
     2401 cagcatcggt cagcggcttt acatactcca gtttgcgact gaaaacggca ttctggcccg
     2461 ccgaaaccac gccaagccgc tcgaccttgt ctttggaggg cgcatcagga cgggcagcat
     2521 taaccagttc caccgcccct ttggtgacgg tgacctctac ggcgttccgc agcagacgga
     2581 catcaaacgc cgttcctatg tcatgtatct gttttgcatt ggcgtggacg gtgaacggcc
     2641 ttttgtcgct gctcgcgatc tcaaaaaacg cttcaccgcg atgcaagtac acgtcgcgcc
     2701 gcgtcttgga ataaaccacg tccagcctgc tgttcgtgtt gagaaccacc gttgacccgt
     2761 ccggcagcga gatgtctttc tgcgagccga gcgcggtttc atatggaatc gcaacgagcg
     2821 gggcgggttg cttgtgcgta tagaacaggc cggcaccggc caggagaaca agcagggcgg
     2881 cggcactggc cacttttcgc caactagcct ctgatcccgc gttccacagt cgccgtgtcc
     2941 atgtctgcct gcgctcgccc gctcccggaa acggtgcggc gaattccttt aaggcatcaa
     3001 actgcgacca taaggccgtc aaccgcaaag ccgcttcccg atggagcgga ctgcggtttt
     3061 gccattcgag aaagcgcgcg taatcctcgg ccggtccgcc ggcgtccagg cggacgatcc
     3121 actccgccgc ctcggcttcc acggtactca cgtccgggaa tttcacgatc ttttcatgat
     3181 catgcatcgc gcacccgttt ccgcggattg accccgagcc ttgtcacaac cccattcggc
     3241 gccttgcctc cgcgatgcgc cttcgttaag tggctggatt tggtgtcgat atcgtaacct
     3301 tggcgacgta gatattcgct gcatcgcaga agaccgagcg ctacgtgctt ttccgccgta
     3361 cttaccgaaa tattcaaacg atatgcgatc gacttgtagg aatagccata taccttccgc
     3421 aatagaaaca cctttgagca ctgcggcggc agactcgcca ccgcttgcgc aagcaagcgt
     3481 acgcgctcgc ggaactccat tttgtcgtca ggagccgcct cgctgtgatc ttctaggaca
     3541 gcttggtcag caaaatcctc cagtgaatcc gtcttcgcat tcgaacattt ctcgcgttcg
     3601 ttcagggcca ggttgcgggc aatccggaac agaaaagcct tgggaaactt caccgtttgc
     3661 gcgctttcgg ctgcaaaggc gcggataaag gtctcctggg ccaagtcgtc tatgtcttcg
     3721 ctgcgtttga tgaatcgctt gagaaaccgt ttaatggcgg cttcgttctc tagatacgct
     3781 tccaggatgg atgacatctt tacacactct ggccgcataa cgagcgatgc agcgggatta
     3841 tagagagcgt cgagagcgaa tagaagtctg caatactgac gcaaggcacg attgcttaac
     3901 ggcgtgccgc ccaaagccgg cggcttctac ggcggccggc tgctgcaaac cgacaccacc
     3961 gtgaacctga atccgaacga cacctatacc ttcagcgcac gccacatcat gcagcagatt
     4021 tccttgcccg ccggcaaggt cgcgattcac gtcg
//
LOCUS       DASWPU010000219         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_112128,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000219 DASWPU010000000
VERSION     DASWPU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112128"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VGC27_04760"
     CDS             complement(<1..484)
                     /locus_tag="VGC27_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEY0281916.1"
                     /translation="MPPLRAVIAAHGLTAKKSLGQNFLLDLNLTRRIARASEPQDAVV
                     YEVGPGPGGLTRALLAEGAACVIAVERDERCVAALADVAEAYPGRLQIVPADALALDE
                     AKLLRACGVKGTVRVAANLPYNIGTALLVKWLTGGALASKSGSLGEPVLRPAVRPKDT
                     W"
     gene            complement(553..1518)
                     /gene="pdxA"
                     /locus_tag="VGC27_04765"
     CDS             complement(553..1518)
                     /gene="pdxA"
                     /locus_tag="VGC27_04765"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF003699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY0281917.1"
                     /translation="MPTSKSAKAGKPPLLVTMGEPAGIGPEVAAAAFAALGGRIEGRP
                     LLVVGDADVFGSCGAVPPEAIVRTKACATRTPGKADAANAAATIEAITLAVEMTLKGE
                     ACAVTTAPINKAVLMQAGFGFPGHTDFLAKLTGAPRAVMMLAGGQLRVIPLTVHIPLA
                     QVPRSIDAASIIETAEIALAALKRDFAIACPRLAIAGLNPHAGEDGELGREETDIIAP
                     AIAELKRRGHLVLGPLPSDTMFHAEARARYDAALCMYHDQALIPIKTLSFWEGVNVTL
                     GLPIVRTSPDHGTGFDIAGKNIADPRSMIAAIRLAAKMADARVRT"
     gene            complement(1496..>2760)
                     /locus_tag="VGC27_04770"
     CDS             complement(1496..>2760)
                     /locus_tag="VGC27_04770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0281918.1"
                     /translation="PTATSGAAADPHHFENEVVALVNDRPISKYDLDQRMALVMSTSN
                     LPSTPEIKQKIRGQVLEQLETELIQRQEALKRDITVSSVEVDRQIQGILTDNHLSMEQ
                     LKAVLARGGVDLSTLRAQIAAQLLWQKTVEQQYAGRVNITPEAVDAEMARIAEGQNKV
                     HYLVSEIFLSVDNPDLDEKVHKDAQSLEAQMAAGAPFAAIARQFSQSPSAAEGGDIGL
                     IYDGQLAPELNAALAKMKTGDLSPPIRSTGGYYILALRQRLEPVGTKIDDSAGQPKEL
                     PSTLPLARLLLPLGPKPSKTMVESALKYAAAITSRVNSCEMLSKVSAEVPGSVYMNFG
                     NARLADLSASVRTALAKTEPGGAAEPFMSDAGVELFVRCDKPVIKQQVYELPKREDVE
                     RKLFSEQISAMARRYNRDLKRNADIEVR"
BASE COUNT          443 a          877 c          905 g          535 t
ORIGIN      
        1 gccaggtatc tttgggtcgc acggccggac gcaaaaccgg ttcgccgagg ctcccacttt
       61 tgctggctaa tgctccgccc gtcaaccact tcaccagcag cgccgtgccg atgttgtaag
      121 gcagattggc ggcgacgcgg accgtgcctt tcacgccgca cgcgcgcaac agctttgctt
      181 cgtcgagtgc cagcgcatcg gcggggacaa tctgaagccg tcccggataa gcctcggcta
      241 catccgcaag tgcggcgacg caccgctcgt cgcgctccac cgcgatcacg cacgcggcac
      301 cttccgccag cagcgcgcgc gttaacccgc cgggaccggg accgacttca tacaccacgg
      361 cgtcttgcgg ttcggaggcg cgcgcgatgc ggcgggtgag attgaggtcg agcaggaaat
      421 tctggccgag cgatttcttc gccgtcagcc cgtgcgccgc gatcaccgcg cgcagcggcg
      481 gcaatttttc ctccccctgc aagggggagg tcggtccgcg caagcgaacc gggtgggggt
      541 ctgatggggc gttcacgtgc gaactctcgc atccgccatc ttcgccgcca gccttatggc
      601 ggcgatcatg ctgcgcggat cggcaatgtt tttgcccgcg atgtcgaagc cggtgccgtg
      661 atcgggcgag gtgcgcacga tgggaagccc cagcgtcaca ttcacgcctt cccagaaaga
      721 gagggtcttg atcgggatca gcgcttggtc gtgatacatg cacaaagcgg cgtcgtagcg
      781 cgcccgcgct tcggcgtgga acatggtgtc ggagggcagc ggacccagca ccagatggcc
      841 gcgccgcttc agttcggcga tggcgggcgc gatgatgtcc gtctcttcac ggcccagttc
      901 gccgtcttct cccgcatgcg ggttgagtcc ggcgatcgcc aatcgcgggc aggcaatcgc
      961 aaagtcgcgt ttcagcgcgg cgagcgcgat ctcggccgtc tcgatgatgg aggcggcgtc
     1021 gatggagcgc ggcacctggg cgagcggaat gtggacggta agcgggatca cgcgcaattg
     1081 gccgccggcc agcatcatca cggcgcgagg cgcgcccgtc agctttgcca ggaagtcggt
     1141 gtgcccggga aagccgaaac cggcttgcat cagcacggct ttgttgatcg gggcggtggt
     1201 tacggcgcag gcctcgccct tcagcgtcat ctcgacggcg agggtaatgg cttcgatggt
     1261 ggcggcggcg ttcgcggcgt cggcttttcc gggcgtgcgc gtggcgcatg ccttggtccg
     1321 gacgatcgct tccggcggaa cggcgccgca gcttccgaac acatccgcat cgcccacgac
     1381 aagcaacggt cttccttcga tgcgtccgcc gagcgcggca aaagcggcgg ccgccacctc
     1441 gggccctatg ccggccggtt cgcccatagt gacgagaagc ggaggtttgc cggctttagc
     1501 ggacttcgat gtcggcattg cgcttcaagt cgcgattgta gcgacgggcc atggcgctga
     1561 tctgttccga gaagagcttg cgttcgacgt cttcgcgttt cggcagctcg tagacctgct
     1621 gcttgatcac gggcttgtcg cagcgcacga acaactcgac gccggcatcc gacatgaacg
     1681 gctccgcggc gccgcccggt tcggtcttcg ccaaggcagt ccggacggag gcgctcaaat
     1741 cggcgagccg cgcattgccg aagttcatgt acaccgagcc aggtacttcg gccgacacct
     1801 tggacagcat ttcgcagctg ttgacgcgac tggtgatcgc cgcggcgtat ttgagggcgc
     1861 tctcgaccat ggtcttggac ggtttcggcc ccaacggcag gaggaggcgc gccagcggca
     1921 gagtgctggg cagctctttc ggctgtcccg cgctgtcgtc gattttcgtg cccaccggct
     1981 ccagtctttg gcgcagggcg agaatgtaat accccccggt cgaacggatc ggcggcgaca
     2041 agtcgccggt cttcatcttg gcaagggccg cgttcagttc cggcgcgagc tgaccgtcgt
     2101 aaatcaggcc gatatccccg ccttcggctg ccgacgggct ctggctaaac tgcctggcaa
     2161 tggcggcgaa gggcgcaccc gccgccatct gggcttcaag gctttgcgcg tccttgtgca
     2221 ccttttcgtc caagtcggga ttgtccaccg acagaaagat ttccgacacg agataatgca
     2281 ctttgttctg gccttcggcg atgcgcgcca tctcggcatc gacggcttcc ggcgtgatgt
     2341 tcacgcggcc cgcatattgc tgttccacgg tcttctgcca aagcaactgg gcggcaatct
     2401 gcgcgcgcag ggtcgacaaa tcgacgccac cgcgcgccaa caccgccttg agctgctcca
     2461 tcgacaggtg gttgtcggtc aggatgccct gaatctgcct gtcgacttcg accgacgata
     2521 ccgtgatgtc cctcttcaac gcttcctgtc gctggatcag ttcggtttcg agctgttcga
     2581 gcacctggcc gcggatcttc tgcttgatct ccggcgtgga tggaagattg gatgtgctca
     2641 tcaccagcgc catgcgctgg tcgagatcgt atttcgagat cgggcgatcg ttgaccaggg
     2701 cgacgacctc gttctcaaaa tggtgcggat cggctgccgc cccggaggtg gccgtcggtg
//
LOCUS       DASWPU010000220         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_44854,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000220 DASWPU010000000
VERSION     DASWPU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44854"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VGC27_04775"
     CDS             complement(<1..507)
                     /locus_tag="VGC27_04775"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY0281919.1"
                     /translation="MLLENVWAMRHRLFEQYSRIEKASGFILFLCAVAALLLANLPKT
                     VAGYHHVLTNTLSLGVPGLHMVTLSTEEWIKEGLMTIFFFVVGIELKHEFLDGELSRP
                     KAALLPIAAALGGMIAPAALYWLLNRPEGLPALSGAAGSAQGWPVPMATDIAFAVAAL
                     SVVARRLPR"
     gene            689..1021
                     /locus_tag="VGC27_04780"
     CDS             689..1021
                     /locus_tag="VGC27_04780"
                     /inference="COORDINATES: protein motif:HMM:NF019019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEY0281920.1"
                     /translation="MTPRLKAGIFVRALIRRAEVAGAQAYVVKKGSEEAGAVFLKVSR
                     LDGTCTILNQVRPGDGELAWAQPLGGPCDEARASAYFAKQMKFDPDLWIVEIEDREGR
                     AFVDERIV"
     gene            1084..2628
                     /locus_tag="VGC27_04785"
     CDS             1084..2628
                     /locus_tag="VGC27_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0281921.1"
                     /translation="MPHTIATEAARRRTFAIISHPDAGKTTLTEHLLLLGGAIHEAGR
                     VKARGEARRARSDWMKIEQERGISVTSAVMTFEYEGAIFNLLDTPGHEDFSEDTYRTL
                     TAADSAVMVIDAAKGIESQTRKLFEVCRLRDIPIMTFVNKMDRESRDAFDLLDEISDQ
                     LQLDVAPMAWPAGHGQNFKGVYDLYADAFRPFGDAALPDDVKDKLEEDMELARGGLAR
                     FDAASYREGHLTPVFFGSALKNFAVKDLLSMLAMHAPPPRPQAARGREVKPAEREVSG
                     FVFKVQANMDPNHRDRVAFVRLASGRFRRNMKLIQSGTGKVIGVHNPILFFAQSRETV
                     DEAFPGDIIGIPNHGTLRVGDTLSESGAIVFTGIPNFAPEILRRVRLGDPMKQKHLAR
                     ALTSLAEEGVTQVFKPAIGNYWIVGVVGPLQLDVLKSRLRDEYELDAELEASPYDAAR
                     WLSAEPADLDKFVESNRGGMAADRDGAPVFLAKSAWEMGYVLEKFPKVRFAKTRERSD
                     VQAERN"
     gene            2760..3059
                     /locus_tag="VGC27_04790"
     CDS             2760..3059
                     /locus_tag="VGC27_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY0281922.1"
                     /translation="MSREYRYFVYMLASKRNGPLYTGVTSDLANRTWQHKSDLIEGFT
                     KRYGVHRLVWFEEFGDIYEAIAREKQIKGWNRAWKIRLIEKQNPEWRDLYSEIIG"
     gene            3112..3387
                     /locus_tag="VGC27_04795"
     CDS             3112..3387
                     /locus_tag="VGC27_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY0281923.1"
                     /translation="MQKLYYVYMLASRRNGTLYTGVTNDINRRVWEHKSDLVEGFTKK
                     YGVHILVWYETHEDINAAIAREANQRMESRLETPAYRERKFRLERSL"
     gene            complement(3473..>3697)
                     /locus_tag="VGC27_04800"
     CDS             complement(3473..>3697)
                     /locus_tag="VGC27_04800"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEY0281924.1"
                     /translation="EHFRVEGRKTWFETIEEMQVVLDEYLVGYNTKRPHQGRGMNGRT
                     PLVAFREGMPKSEAKKEKIKPEKQPNQIAA"
BASE COUNT          758 a         1114 c         1145 g          680 t
ORIGIN      
        1 acgcggcagc ctgcgcgcga ctaccgagag cgcggcgacg gcaaaggcga tgtcggtcgc
       61 catcggcacc ggccatcctt gcgcgctgcc ggccgcgccg gaaagtgccg gcaatccttc
      121 cggccggttg agcagccaat agagcgccgc cggggcgatc atcccgccca gcgccgctgc
      181 gatcggcagc agtgccgcct tcggccggga caattcgccg tcgagaaatt cgtgcttcag
      241 ttcgatgccg acgacgaaga agaaaatcgt catcagccct tccttgatcc actcttccgt
      301 ggaaagcgtc accatgtgca gcccgggaac gcccagcgac agggtgttcg tcagcacatg
      361 gtgataaccg gccaccgtct taggcaggtt ggccaggaga agggctgcca cggcgcagag
      421 gaaaagtatg aagccgcttg ccttctcaat gcggctgtac tgctcgaaca accggtgcct
      481 catggcccac acattttcca gcagcatcgg cggtcttcct tttacctgcc gttcgacaga
      541 atgcgcgaaa tgaaatcagc gccagctttg tgtctatatc gcgcaaaagc caatttgcag
      601 caattacgcc gcgaacgccg gcttccggaa gcggcggccc ggccgtgacg gcttgaaacc
      661 ggtggcaggc ggcggctaaa acctgttcat gactcctcgc ctcaaagccg gcatcttcgt
      721 ccgcgccctc atccgccgcg cggaagtggc cggcgcgcag gcctatgtcg tgaagaaggg
      781 gtccgaggaa gccggcgcgg tgttcctgaa ggtctcgcgg ctggacggca cttgcaccat
      841 cctcaatcag gtgcgcccgg gcgacggcga actggcctgg gcgcagccgc tgggcggccc
      901 atgcgacgag gcgcgcgcct ccgcctattt cgccaagcag atgaagttcg atcccgatct
      961 gtggatcgtg gagatcgagg accgcgaagg ccgcgccttt gtggacgaga ggatcgtgta
     1021 ggtaaaacgc gaatagcgaa tagggagtag cgaacagcaa gggtttctgc tattcgccac
     1081 cccatgccgc acaccatcgc caccgaagcc gcgcgccggc gcaccttcgc catcatcagc
     1141 cacccggacg ccggcaagac cacgctgacc gagcatctgc tcttgctcgg cggggcgatt
     1201 cacgaggccg gccgcgtcaa ggcgcgcggc gaggcgcggc gcgcccgttc ggactggatg
     1261 aagatcgaac aggagcgcgg catctcggtg accagcgcgg tgatgacctt cgaatatgag
     1321 ggcgccatct tcaatctgct cgatacgccg ggccatgagg atttctccga ggacacctac
     1381 cgcacgctga ccgccgccga ttccgcggtg atggtgatcg acgccgccaa gggcatcgaa
     1441 agccagaccc gcaaactgtt cgaggtctgc cgcctgcgcg acatcccgat catgactttc
     1501 gtcaacaaga tggaccgcga atcacgcgac gccttcgacc tgctcgatga aatctccgac
     1561 cagttgcagc tcgacgtggc gccgatggcc tggcccgccg gtcacggcca gaacttcaag
     1621 ggcgtctacg atctttatgc cgacgcgttc cggcccttcg gcgatgcggc gctgccggat
     1681 gacgtcaagg acaagctcga agaggacatg gaactggcgc gcggcgggct ggcgcgcttc
     1741 gatgctgcct cctatcgaga agggcacctc acgccggtgt ttttcggttc ggcgctgaaa
     1801 aatttcgcgg tcaaggattt gctgtcgatg ctggccatgc atgcgccgcc gccgcgcccg
     1861 caagccgcca gaggccgcga agtcaaaccg gccgagcgcg aggtttccgg cttcgtcttc
     1921 aaggtccagg ccaacatgga ccccaaccat cgcgaccgcg tggccttcgt gcggctggcg
     1981 tccggccgct tccgccgcaa catgaagctg atccagtcgg gcaccggcaa ggtgatcggc
     2041 gtgcacaacc cgatcctgtt cttcgcccag agccgcgaga cggtagacga agcctttccc
     2101 ggcgacatca tcggcattcc caatcacggc accttgcgcg tcggcgacac gctgtcggag
     2161 tccggcgcca tcgtcttcac cggcatcccg aatttcgcgc cggagatttt gcgccgcgtg
     2221 cggctgggcg atccgatgaa gcagaagcat ctggcccgcg cgctgaccag cttggccgaa
     2281 gaaggcgtca ctcaggtgtt caagccggcc atcggcaatt attggatcgt gggcgtggtg
     2341 gggccgttgc agctcgacgt gctgaaatcg cggctgcgcg acgaatacga actcgacgcc
     2401 gaactggaag cctcgcctta cgacgccgcg cgctggcttt ccgccgagcc ggccgatctg
     2461 gacaagttcg tcgaatccaa tcgcggcggc atggccgccg accgcgacgg cgcgccggtg
     2521 ttcctggcca agagcgcgtg ggaaatgggc tacgtcctgg agaaattccc caaagtgcgc
     2581 ttcgccaaaa cccgcgaacg cagcgacgta caggcggagc gcaattaaaa taggctgtca
     2641 ttcccggcga gccgcgcgct tcgcgcggcg agggaaggga atccagaaac ctgcgcggac
     2701 ttatggattc ccttcccctc gctacgctcg gccgggaatg acaggtatat agttgtcgta
     2761 tgagccggga atatcgctac tttgtgtata tgcttgcgag caaacggaat ggcccgctct
     2821 atacgggagt gaccagcgat ctcgcaaatc gaacttggca gcataaaagc gatctgatcg
     2881 aaggattcac gaaacgctat ggcgttcatc gcctcgtttg gtttgaagaa ttcggcgata
     2941 tctatgaggc gatagcgcgc gagaaacaga tcaagggttg gaacagagcc tggaaaatcc
     3001 gcctgatcga aaaacaaaat ccggaatggc gagacctcta cagcgaaatt ataggttgat
     3061 tgtcattccc ggcgagccgc gcgcttcgcg cggcgaggga agggaatcca ggtgcaaaag
     3121 ctttactacg tctacatgct cgcgagcagg cgaaacggaa cgctgtatac aggcgtgact
     3181 aatgatatca acagacgcgt ttgggaacac aaaagcgatc tggtcgaagg gttcaccaag
     3241 aaatatggcg tccatatcct tgtctggtac gagacgcatg aagacatcaa cgcggcgatt
     3301 gcgcgagaag caaatcaaag gatggaatcg cgcttggaaa ctccggctta tcgagaaaga
     3361 aaattccggt tggaacgatc tttatgagaa actcggatag atcgcatttg gctgcagcac
     3421 ctgggtgtac agagagggta atcggctgac aggtgccgct ctttgagcgg gcctaggcgg
     3481 cgatctggtt aggctgtttt tcgggtttga tcttctcctt tttggcttcg gatttgggca
     3541 ttccttcgcg gaaggctacg agcggggtgc gcccgttcat gccacggccc tgatgtgggc
     3601 gcttggtatt gtagccgacg aggtactcgt cgagaaccac ctgcatctct tcgatggttt
     3661 cgaaccaggt cttgcgtccc tcgacgcgga agtgctc
//
LOCUS       DASWPU010000221         5892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_32593,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000221 DASWPU010000000
VERSION     DASWPU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32593"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..194
                     /locus_tag="VGC27_04805"
                     /pseudo
     CDS             51..194
                     /locus_tag="VGC27_04805"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            558..770
                     /locus_tag="VGC27_04810"
                     /pseudo
     CDS             558..770
                     /locus_tag="VGC27_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751481.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            900..3176
                     /locus_tag="VGC27_04815"
     CDS             900..3176
                     /locus_tag="VGC27_04815"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY0281925.1"
                     /translation="MAITTAVEKPVRGVSGVLRAISRPSLLALGVAMLALLSALFTYA
                     ILTGLTPFEATPTVLGVLLLSNLTLGLSLGALMAWRLMRLWKERRSGRAGARLHVRLV
                     AMFSAVAVVPAILVAIFAAITLNLGVQAWFSGHVKTAIDSAENVARLYVLERARGITM
                     DAGEIAAGIENDPNIFDLKEKKVHLGLMFAKLEDMTRDHGLQASYIIDSHGVVLGSSK
                     QKEWLSMPDPSPPKPSEIGQAAQGNIFVDADPEVGVVRALIRLRALQDAYLLVVRKVD
                     PTVFNYYQRTKNTVHEYNRLEENLSGVQLVFATMYGLVSLVILLAAIWLGLWAADRLV
                     KPISRLIGAAERVSEGDLKAQVEVDRDDDEIGTLGLAFNRMTQQLDSQRSALVAANRQ
                     IDKRRRFTETVLGGVSAGVIGLDADGQITIVNRAAARLLDAAPEELEGQHYAGAVPEL
                     AALIRRALQEPAARAGGEVTVKRAGTTRSLSVQVTSEHGHHGSGYVATFDDITDLVSA
                     QRTAAWADVARRIAHEIKNPLTPIQLSAERLKRKYGGQVGADREVFEQCTDTIIRQVG
                     DIGRMVDEFSSFARMPTPVMRRECAQELLQQAVFLQRVAHPQITFETRIPRELVWLEC
                     DGRLVSQALTNVLKNATESIAARGAAGDDTPGRIAVAIEASDTQVAIKVFDNGIGLPP
                     EHRHRLTEPYVTTRAKGTGLGLAIVRKIMEDHGGEIVLEDAAEDGKGAQVTLSFPLIK
                     TSREKGVSDEQTRIATRV"
     gene            3145..4560
                     /locus_tag="VGC27_04820"
     CDS             3145..4560
                     /locus_tag="VGC27_04820"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0281926.1"
                     /translation="MSKHGLPLASEILVVDDEEDIRDLIAGLLRDEGYETRVAGDSDG
                     VLAAVRQRRPQLVVLDIWLQGSRLDGIQVLDTLKREQPDLPVVMISGHGTIETAVASI
                     KKGAYDFIEKPFKADRLIHVVTRALEAARLRREVQELKLKAGDETDLVGSSSAVSQIR
                     QAVEKIAPTNSRVFISGPPGSGKELLARLIHARSRRAANAFVAVNCATMEPDRIETEL
                     FGVENEDGPRKIGLLELAHNGTLYLDEVEDMPLETQGKVLRVLVDQTFTRVGGSTRVQ
                     VDARVISSASRDMRPEIVAGRFREDLYHRLNVVPVRVPSLAERRDDIPLLVAHFMKRL
                     SAASGLPMREIGDDAMAVLQAHSWPGNVRQLRNIVERLLILATDDTTQAISADLLPSD
                     LGTSVQWGGTGRSDLVISMPLREAREIFERDYLVAQINRFGGNISRTAAFIGMERSAL
                     HRKLKSLGVGSSMGVRESFNA"
     gene            4591..5517
                     /locus_tag="VGC27_04825"
     CDS             4591..5517
                     /locus_tag="VGC27_04825"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF005209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEY0281927.1"
                     /translation="MSRWPSHPPAGRIAYVDGRYVRHGEAGVHIEDRGLQLGDSVYEV
                     CRIADGRLIDIEEHLDRLERSLGEIEMAMPMGRGALKSVMAEIARRNGMRDGLLYLQV
                     TRGALRRDHPIPAAAPKPTLILTVHPVDQAAVARRHAEGVAIITCPDERWGRCDIKST
                     QLLANLLAKTAAHRAGVYEAWLVGQDGFVTEGASTTAWIVDGEGRVVTRDLSRAILPG
                     VTRRVILEAAAEAQMPVLERSFTREEAAGAREAFLSSATGGAVPVVAIDGRKVGDGRP
                     GPLTRRVMALYAQHSQEPVAQRKLRRNPHIRI"
     gene            5606..5860
                     /gene="hfq"
                     /locus_tag="VGC27_04830"
     CDS             5606..5860
                     /gene="hfq"
                     /locus_tag="VGC27_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY0281928.1"
                     /translation="MSEKQQNLQDTFLNAVRKSKSSVTIFLVNGVKLQGNITWFDNFC
                     VLLRRDGQAQLVYKHAISTVMPMGPIQLQEQETALETTEA"
BASE COUNT         1079 a         1852 c         1930 g         1031 t
ORIGIN      
        1 gtttcgaaca ggccgagggc ggcacgctgt ttctcgacga gatcggcgac atgccgctgg
       61 atgcgcagac gcgtctgctg cgcgtgctgc agcaaggcga atacacgacg gtcggcgggc
      121 gcacgccgat gaagaccgac gtgcgcatca tcgccgcgtc gaaccgcgat ctgcgccagc
      181 tcatccagca gggcctgttc cgcgaagacc tctattaccg gctcaacgtc gtgccgctgc
      241 gcctgccgcc gctgcgcgag cgcgtcgagg acatccccga cctggtgcgc cacttcctgc
      301 gcaaggccga ggaagagggc ctgccggcga agcatctcga cgccgacgcg cttgacatcc
      361 tgcggcacca ccgttggccc ggaaacgtcc gcgaactgga gaacctgatc cgccggctgg
      421 cggttctgca ttccggggag acgatccccg ccgccgccat cgccgcggag ctgaaggaac
      481 cggccagggc gagcagcctc gacgacggcg acgagccggt ttcgctgagc gcggccgtcg
      541 agcatcattt gacgaaatac ttcgccgaac atggggagcg tctgccgccg cccggtctgt
      601 atgatcgcgt cctgcaagac atcgaacggc cgctgctgtc gatctgtcta gcggcgacgc
      661 gcggcaacca gattcgggca gcccacctcc tgggactgaa tcgcaacacg ctgcgcaaga
      721 aaatccgcga cctggggctt gaggtcatca ggggtttaaa ggcggattag gccgtgattt
      781 aaccctgttg ttggattgca tcgcagcatt cttgcaacag gttgccagat cgctacacta
      841 cgtaattaca aagtgacgct cgcgggttag cgggccgcat ccgtcaaaga cggtcgggaa
      901 tggccataac gacagcagtc gaaaagccgg tacgcggtgt gtctggggtc ctgcgcgcga
      961 tatcgcgccc gtccctgctg gcgctgggcg tggcgatgct ggctctgctg tccgctctgt
     1021 ttacttatgc catcctgacg gggctgacgc cgttcgaggc gacgccgacg gtcctaggcg
     1081 tgctccttct ttcaaacttg acgctaggtc tgtcgctcgg cgcgctgatg gcatggcggc
     1141 tgatgcggtt gtggaaagag cgccgctcgg gcagggcggg ggccaggctg catgtgcgtc
     1201 tggtggcgat gttcagcgcc gtcgccgtcg taccggcgat tctggtggcg atcttcgcgg
     1261 cgataaccct caacctcggc gttcaggcct ggttctccgg gcatgtgaaa acggcgatcg
     1321 acagcgccga gaacgtggcc cgcctttatg ttctggaacg ggcccgcggc atcaccatgg
     1381 acgccggcga gatcgcggcc gggatcgaga acgatccgaa catcttcgat ttgaaggaaa
     1441 agaaagttca tctcgggctg atgttcgcca agctcgagga catgaccagg gatcatggcc
     1501 tgcaagcgtc ctacatcatc gacagccatg gcgtggtgtt gggcagttcc aagcagaagg
     1561 aatggctgtc catgcccgat ccgtcgccgc ccaagccgag cgaaatcggg caggcggccc
     1621 aaggcaacat cttcgtcgat gccgacccgg aggtcggcgt tgtgcgcgcc ttgataagat
     1681 tgcgcgcgtt gcaggacgct tatctgctgg tcgtgcgcaa ggtcgatccg acggtgttca
     1741 actattacca gcgcaccaag aacacggtcc acgaatataa tcgcctggaa gaaaacctct
     1801 cgggcgtgca gctcgtcttc gcgaccatgt acggactggt gtcgctcgta atcctgctgg
     1861 ccgcgatctg gctgggtctg tgggccgccg accgtctggt gaagccgatt tcgcgcctga
     1921 tcggggcggc ggagcgcgtc tcggaaggcg atcttaaggc gcaagtggag gtcgatcgcg
     1981 acgacgacga gatcggaacg cttggtctgg ctttcaaccg catgacccag caactggatt
     2041 cgcagcgaag cgcgctggtc gccgccaacc gtcagatcga caagcgccgc cgcttcaccg
     2101 agaccgtgct gggcggcgtc agcgccggcg tgatcggcct cgacgcggac ggacagatca
     2161 ccatcgtcaa tcgcgccgcc gcgcgtcttt tggacgccgc tccggaggaa ttggaaggcc
     2221 agcattacgc gggggcggtg cccgaactgg cggctctgat ccgtcgcgct cttcaggagc
     2281 ctgcggcgcg cgccgggggc gaggtcacgg tcaagcgcgc cggcaccacg cgttcgctca
     2341 gcgtccaggt caccagcgaa cacggccatc acggcagcgg ctacgtcgcc accttcgacg
     2401 acatcaccga ccttgtgtcc gcccagcgca ccgccgcctg ggccgacgtc gcgcgacgca
     2461 tcgcccacga gatcaagaac ccgctgacgc cgatccagct ttccgccgaa cggctgaaac
     2521 gcaaatatgg cggccaggtc ggcgccgacc gcgaagtgtt cgagcagtgc accgatacga
     2581 tcattcgtca ggtcggcgac atcgggcgca tggtcgacga attctcgtcc ttcgcgcgga
     2641 tgccgactcc ggtcatgcgg cgcgaatgcg cgcaggagct tttgcagcag gcggtgttcc
     2701 tgcagcgcgt ggcccatccg caaataactt tcgagacccg catccccagg gaacttgtct
     2761 ggctcgagtg cgacgggcgg ctggtgagcc aggcgctgac caacgtgctc aagaacgcca
     2821 cggaaagcat cgccgcgcgc ggcgccgcgg gcgacgatac gccgggtcgt atcgcggtcg
     2881 cgatcgaagc gtccgatacg caggttgcga tcaaggtctt tgacaacggc atcggcctgc
     2941 cgcccgagca tcgccaccgt ttgacggaac cttatgtgac gacccgcgcc aagggtacgg
     3001 gacttggtct cgccatcgtg cgcaagatca tggaagacca tggcggcgag atcgttttgg
     3061 aagatgcggc ggaagacgga aagggagcgc aggtaacgct gtcgttcccg ttgattaaaa
     3121 cttcgcgtga gaagggtgta agcgatgagc aaacacggat tgccactcgc gtctgaaatc
     3181 cttgtcgtcg acgacgaaga ggatattcgc gatttgattg ccgggctgct gcgcgacgag
     3241 ggctatgaaa cccgcgtggc cggcgacagc gacggggttc tggccgccgt ccggcagaga
     3301 cggccgcaac tggtggtgct ggacatctgg ctgcaaggca gccggctcga cggcatccag
     3361 gtgctcgata cgctcaagcg cgaacagccc gacctgccgg tggtgatgat ctccggccac
     3421 ggcaccatcg aaaccgccgt cgcgtcgatc aagaagggcg cctatgattt catcgagaaa
     3481 ccgttcaagg ccgacaggct gatccacgtc gtcacccgcg cgctggaagc cgcgcggctg
     3541 cggcgcgaag tgcaggaact caagctcaag gccggcgacg aaaccgacct ggtgggctca
     3601 tcgtccgccg tgtcgcaaat ccgccaggcg gtggaaaaga tcgcgccgac caacagccgc
     3661 gtgttcatct ccggcccgcc gggttccggc aaggaacttc tggcccgcct gattcatgcg
     3721 cgctcgcgcc gggcggcgaa tgcgtttgtg gccgtcaatt gcgcgacgat ggagccggat
     3781 cgcatcgaaa cggagctgtt cggcgttgaa aacgaggacg gcccgcgcaa gatcggcctt
     3841 ctcgagttgg cgcataacgg cacgctttac ctcgacgagg tggaggacat gcccctggaa
     3901 acccagggca aggtcttgcg cgtgcttgtg gaccagacct tcacccgcgt cggcggctcg
     3961 acgcgcgtgc aggtggacgc gcgcgtcatc agttccgctt cgcgcgacat gcgcccggaa
     4021 atcgtcgccg gccgtttccg cgaggacctt taccaccgct tgaacgtcgt gccggtgcgc
     4081 gtgccttcgc tggccgaacg ccgcgacgac atcccgctgc tggtcgcgca tttcatgaag
     4141 cggctgtcgg cggcgtccgg actgcccatg cgcgagatcg gcgacgatgc gatggccgtg
     4201 ctgcaagccc atagctggcc cggcaatgtc cgccagcttc gcaacatcgt ggagcgcttg
     4261 ctgatcctgg ccaccgacga caccacgcag gcgatttcgg cggatcttct gccctcagat
     4321 ctgggcacca gcgtgcaatg gggcggaacc ggccgcagcg atctcgtcat ttcgatgccg
     4381 ctgcgcgaag cgcgcgagat tttcgagcgc gattacctgg tggcgcagat caaccgcttc
     4441 ggcggcaaca tttcgcgcac cgccgcgttc atcggcatgg agcgctccgc gctgcaccgc
     4501 aagctcaagt cgctgggcgt gggatcgagc atgggtgtcc gggaaagctt caatgcatag
     4561 cgttgcctcc ccctttgtgg ggaggcgatc ttgagccgct ggccttcgca cccgcccgcc
     4621 ggccgcatcg cttatgtcga cggccgctat gtacgccacg gcgaggccgg cgtgcatatc
     4681 gaggatcgcg gtctgcaact cggcgattcc gtttatgaag tctgccggat cgcggacggg
     4741 cgcctcatcg acattgagga acatctggac cgtctcgaac gctcgctggg cgagatcgag
     4801 atggcgatgc cgatgggccg cggcgcgctc aaatccgtga tggccgagat cgcacggcgc
     4861 aacgggatgc gcgacggtct gctttacctt caggtcacgc gcggcgcgct gagacgcgac
     4921 catccgattc cggcggcggc gcccaaaccg acgctgatcc tcaccgtgca tcccgtggat
     4981 caagctgccg ttgcgcggcg ccatgccgaa ggcgtcgcca tcatcacctg tcccgacgag
     5041 cgctggggcc gctgcgacat caaatcgacg caacttttgg caaatctgct cgccaaaacg
     5101 gcggcgcacc gggcaggggt gtatgaggca tggctggttg gccaggacgg attcgtgacc
     5161 gaaggggcct ccaccaccgc ctggatcgtc gatggcgagg gccgtgtcgt cacccgcgat
     5221 ctcagccggg ccatcctgcc cggggtgacg cggcgggtca tactggaggc cgccgccgag
     5281 gcgcaaatgc ctgtcctgga gcgcagtttt acgcgcgagg aagccgccgg cgcgcgggag
     5341 gcgtttctgt cgtcggccac cgggggcgcg gtcccggtcg ttgccatcga tggccggaaa
     5401 gtgggggacg gcaggcccgg cccgctgacg cgccgggtta tggcgctata tgcgcaacat
     5461 tctcaagagc cggttgcaca aagaaaattg cgtcgcaatc cccatatccg catctagggc
     5521 tttcgctggc gcaatagtcc gccttatgct gcctttactg cttcgcaggg gtcgcagggc
     5581 caataaccag acgggtataa gatcaatgag cgaaaaacag caaaacctgc aagatacgtt
     5641 tcttaacgcg gtcaggaaga gcaagtcgtc ggtgacgatc tttctcgtca atggcgtcaa
     5701 gcttcaaggg aacatcactt ggttcgacaa cttctgcgtg cttctgcgtc gcgacgggca
     5761 ggctcagctc gtctacaagc acgcgatctc gacggtcatg ccgatggggc cgatccagct
     5821 ccaggagcag gaaaccgctc tcgaaaccac ggaagcttga caagcggaca tgagtccggt
     5881 gcgcgccggc gc
//
LOCUS       DASWPU010000222         8315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_118199,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000222 DASWPU010000000
VERSION     DASWPU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8315
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118199"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VGC27_04835"
     CDS             <1..911
                     /locus_tag="VGC27_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HEY0281929.1"
                     /translation="GMARTFRVFLAQPDQIGIGPGELLGLMRELLGRLALILGPVFGV
                     MMIAGVAGNMLQHRPVFTFEKIKPSFSKISLLSGFKRLFGVDGFANLFKGMLKIVIVS
                     VAVWTQLWPERGGFETVLQQSPAGVAGDMAHFMFKVLIAALVAMTAIAGADYALQRFQ
                     FLKRNRMSKHEIKEEYRQTEGDPMIKAKIRQLRQERARRRMIAAVPEATVVIVNPTHF
                     AVALKYESGKMAAPICVAKGVDALALRIREVAEKHAVPIVENPPLARTLHAAVEVDEP
                     IPPEHYKAVAQVIGYVMRLTGKLRAN"
     gene            954..3062
                     /locus_tag="VGC27_04840"
     CDS             954..3062
                     /locus_tag="VGC27_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0281930.1"
                     /translation="MATRSIIGGNLGVPAGGWRWAALGFAVLALGAAGLALATPDAAR
                     WAGVALLCGIGALGSLFLYAVWPREAMSAADARRVAEAAARANVAWAITGADGAVLDS
                     NDVYRRMAGTAEGESAAPPELALAGDPSAAVLYRLTRCAAEGVAREESFTVAPGLEIV
                     AAVRPLAGGQTVWWFTPRLSPGAHAPAKPAAAPAPANFSLDDVFRDAPMGVAFADAEG
                     KLVDANAAFKTFFGSAGALSGRALAGLVEASDRDSVKALIAGAAEGTANGSSVELRAA
                     GQGERMAELFAGPANSGRVTLYLLDVSEQKALEVKFAQSQKMQAVGQLAGGVAHDFNN
                     LLTVIIGNSEFLLMRHQAGDPSFKDINEIHQNALRGATLVGQLLAFSRKQTLQPRVLM
                     VRDVVGELALMLRRLLREGVELKLEHGADLWPVHADEAQLSNAIINLVINARDAMPDG
                     GTVTIRTANETATAPVALGTAIMPAGDYLRIEVADTGTGIAKEHLGKIFDPFFTTKPV
                     GQGTGLGLATVYGIVKQTGGFITVDSDIGKGTVFRIFLPRFRADQSVVQPEPERAAPR
                     DVTGQDTILLVEDEEAVRSFAARALRMRGYNVIEASGGEDALEIVRAAAAPIHLLITD
                     VVMPNMDGPTLVRAVRRIRPDMAVIFMSGYAEESFRRNDEKAEDLHFLPKPFGLKQLA
                     GKVKEVLSGAPAPKRAQMGE"
     gene            complement(3257..5878)
                     /locus_tag="VGC27_04845"
     CDS             complement(3257..5878)
                     /locus_tag="VGC27_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0281931.1"
                     /translation="MRFAFRARLSRLRVGLQPSAIAGALILAFIVLLWSGYAWLTVSE
                     YSDRLAEEQRNLYTAARAYADYAGMLTTIDISIPVGDASDRNPMSAGLEYGARELARF
                     RSDLNLPSGTTLHLDSIDESKASPRVNGDMLVARAANPAAGYVVVAQRPVGNAVEEWR
                     RGALVEGGGLAAITLLMVALSLVLLRQMRRHEAMEGQLIAAKELAEAGNRTKSEFLAN
                     MSHEIRTPLNGVLGMAGLLLGTDLDEEQRKFAETIQESGDTLLAVVNDVLDFSKLEAG
                     KFEIDRIEFDLVSTVENAVKLMASKAREKGIDLGVFIEPAARGAYMGDPTRIRQILLN
                     LLSNAIKFTDRGGVSVQVNVRLGETMLPDGVVPLRFEVTDTGIGMPDSVRERLFQKFS
                     QVDNSMTRRYGGTGLGLAICRQLIDLMGGRIDVKSRLGAGSTFWFELALARIGTTVVD
                     RDDLTAQFKALRALIVDDVAMNLEILGRQLHALGMESLGVDDGFAAIAELEREWHRGK
                     PYDIVFLDQMMPGISGSELARRIRQSDHLAECKLVMVSSAGREAIRSQTSVKFDAILE
                     KPVRQQDLLDCLIGIYSIHIEPAAVAVYRRDSKAAAAAAKRKLRVLVAEDNRVNQQYA
                     RALLEKAGHFVEIVDNGHKAVDALRRDDFDVVLMDIQMPELDGVQATQQIRKLSMPKA
                     AIPIIAMTAHAMKGAREEYLAAGMDDYIAKPVQPTLLLTKLAAIAERLPRVIPAAVEL
                     SPPTPDGGSEGVLDQEKLQTLDAVLSFAEATAFVEMFLADADSHMAAIADAAQRADYA
                     AIARSAHVLVSTAGNVGATAVSLGARHLTDACRKDDRISAEAAMTELEAAYRVASGAL
                     RVWVAGRSAQAQAAERA"
     gene            6648..7718
                     /gene="recA"
                     /locus_tag="VGC27_04850"
     CDS             6648..7718
                     /gene="recA"
                     /locus_tag="VGC27_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY0281932.1"
                     /translation="MKEAVLRVVGKEDGTDKKRALEAARSQIDRAFGKGSVMKLGQRD
                     PVTEADTVSTGSLGLDIALGIGGLPRGRVIEIYGPESSGKTTLALHAVAEVQKKGGIA
                     AYVDAEHALDPGYAKKLGVDIDELLISQPDTGEQALEITDTLVRSGGVDIVVVDSVAA
                     LTPKAELEGEMGDQLPGLQARLMSQALRKLTGSISKSNTIVIFINQIRMKIGVMFGNP
                     ETTTGGNALKFYSSVRLDIRRIGAIKDHDEVVGNQTRVKVVKNKVAPPFKQVEFDIMY
                     GHGISKSGELLDLGVKAGTVEKSGSWFSYDGTRIGQGREQAKAYLEANKDTAAKIEAA
                     IRQNAGLIGQSLALGSVEAAEG"
     gene            7998..>8315
                     /locus_tag="VGC27_04855"
     CDS             7998..>8315
                     /locus_tag="VGC27_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEY0281933.1"
                     /translation="MKSLSDIRAGFLEFFRDRQHAVVPSSPLVPRNDPTLLFTNAGMV
                     QFKNVFTGAEKRPYSRATSVQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KEDA"
BASE COUNT         1530 a         2687 c         2647 g         1451 t
ORIGIN      
        1 tcggaatggc ccgcaccttc cgcgtcttcc tggcccagcc cgaccagatc ggcatcggtc
       61 cgggcgagct gttgggcctg atgcgcgagc tgctgggaag gctggcgctg atcttgggtc
      121 cggtcttcgg ggtcatgatg atcgctggcg tggccggaaa catgctccag caccgtccgg
      181 tcttcacctt cgaaaagatc aagccgtctt tctccaagat ctcgctgctg tcgggcttca
      241 agcggctgtt cggggtcgac ggcttcgcca atctgttcaa gggcatgctc aagatcgtca
      301 tcgtcagcgt ggcggtttgg acccagttgt ggccggagcg cggcggcttc gaaaccgtgc
      361 tgcagcaatc cccggccgga gtcgccggcg acatggccca tttcatgttc aaggtgctga
      421 tcgcggcgct ggtggcgatg acggccatcg cgggggcgga ctatgcgctg caacgcttcc
      481 aattcctcaa acgcaaccgc atgtccaagc acgagatcaa ggaggaatac cgccagaccg
      541 aaggcgaccc catgatcaag gccaagatcc gccagctccg ccaggaacgg gccaggcgcc
      601 ggatgatcgc ggcggtgcct gaggccaccg tcgtcatcgt caacccgacc catttcgccg
      661 tcgccctcaa gtacgaatca ggcaaaatgg ccgccccgat ctgcgtggcc aaaggtgtgg
      721 acgccttggc cctgcgcatc cgcgaggtgg ccgaaaagca tgccgtgccc atcgtcgaga
      781 acccgcccct ggcgcgcacc ctgcacgccg ccgtcgaggt cgacgagccg attccgcccg
      841 agcattacaa ggccgtggcg caggtcatcg gctatgtcat gcggcttacc ggcaagctcc
      901 gggcgaatta attcgcctac ctcggtcccg ctttcggcta gactcggccc gtaatggcga
      961 ctcgcagcat cataggcggc aatctcggtg tcccggccgg aggctggcgc tgggcggccc
     1021 tcggcttcgc cgttctggcg ctgggtgcgg cggggctggc gctggcgacg cccgatgcgg
     1081 cgcgctgggc cggagtggcg ctgctctgcg gcatcggagc gctcggcagc ctgttcctct
     1141 atgcggtgtg gccgcgcgaa gcgatgagcg ccgccgacgc ccgccgcgtc gccgaagcgg
     1201 cggcgcgcgc caacgtcgcc tgggcgatca ccggggccga cggcgcggtg ctcgacagca
     1261 acgacgttta ccggcgcatg gcgggcaccg ccgaaggcga atccgctgcg ccgcccgaac
     1321 tggccctggc cggagacccg tccgccgccg tgctctatcg cctgacgcgc tgcgccgccg
     1381 aaggcgtcgc ccgcgaggaa tccttcaccg tggcgccggg gctggagatc gtcgcggcgg
     1441 tgcggcccct ggccggcggg cagaccgtgt ggtggtttac gccccgtctt tcgcccggcg
     1501 cgcacgcacc ggcaaagccg gcggcggcgc cggcgccggc gaacttcagt cttgatgatg
     1561 tcttccgcga cgctccgatg ggagtagctt tcgcggacgc ggaggggaaa ctggtcgatg
     1621 ccaacgcggc cttcaagacg ttcttcggct ccgccggagc gctgtccgga cgggcgctgg
     1681 ccggtctggt cgaggcttcg gaccgcgaca gcgtcaaggc cctgatcgcc ggggcggcgg
     1741 aaggaaccgc caacggcagc tccgtggaac tgcgcgcggc gggccagggc gaacgcatgg
     1801 cggaactttt cgccggaccg gcgaacagcg gccgcgtgac cctctatttg ctcgacgttt
     1861 cggagcagaa ggcgctggaa gtcaaatttg cccaatcgca gaagatgcaa gcggtggggc
     1921 aattggccgg cggcgtggcg cacgatttca acaatctgct caccgtcatc atcggcaatt
     1981 ccgaattcct cctgatgcgc catcaggcgg gcgacccctc gttcaaggac atcaacgaaa
     2041 tccatcagaa tgccttgcgc ggagcgacgc tggtgggaca gcttctcgcc ttctcgcgca
     2101 agcagacgct gcaaccgcgc gtgctgatgg tgcgcgacgt ggtgggcgaa ctggcgctga
     2161 tgttgcgaag actattgcgc gaaggggtcg aactgaagct ggagcacggc gccgatctgt
     2221 ggcccgtaca cgccgacgag gcccagctct ccaacgccat catcaatctg gtgatcaatg
     2281 cgcgcgacgc catgccggac ggcggcaccg tcaccatccg caccgccaac gagacggcga
     2341 cggcgccggt ggcgctgggc accgcgatca tgccggccgg cgattatctg cgcatcgagg
     2401 tcgccgacac cggcaccggc atcgccaagg agcatctcgg caagatcttc gatcccttct
     2461 tcaccaccaa gccggtgggc cagggcaccg ggctggggtt ggccaccgtt tacggcatcg
     2521 tcaagcagac gggcggcttc atcaccgtgg attcggatat cggcaagggt acggtgttcc
     2581 gcatcttcct gccgcgcttc cgcgccgatc agagcgtggt ccagccggaa ccggaacgcg
     2641 ccgcaccgcg cgacgtcacc ggccaggaca ccatcctcct ggtcgaagac gaagaggccg
     2701 tgcgcagctt cgcggcgcgc gcgctcagga tgcgcggcta caatgtgatc gaagcttcgg
     2761 gcggcgagga cgcgctggag atcgtgcgcg cggcggcggc gccgatccac cttctcatca
     2821 ccgacgtggt gatgcccaac atggacggcc cgacgctggt gcgcgcggtg cgccgcatcc
     2881 gccccgatat ggcggtgatc ttcatgtcgg gctatgccga agaatccttc cgccgcaacg
     2941 acgaaaaggc cgaagacctg cacttcctgc ccaagccctt cgggctgaag cagcttgccg
     3001 gaaaggtgaa ggaagtgctg tccggcgctc ccgcgcccaa gcgggcgcag atgggggaat
     3061 agcagtctcc attagagttt tttcggatcg agcccaccca tatttgccca ccatggccgg
     3121 gcgtccgcac gctggcgcta cggaccccgg ccatccatgg atgggcggct caaagccgcc
     3181 catggtgagg tggcttacgg caggcgattt atctcattcc taaaccgctt cggacacgat
     3241 tgccgttcgc gcggcgctat gcccgttcgg cggcttgcgc ctgggcggag cgcccggcca
     3301 cccagacgcg caaggcgccg gatgccacgc gataggcggc ttcaagttcc gtcattgcgg
     3361 cttcggcgga gatgcgatca tctttgcggc aggcatccgt gagatggcgc gcgccgaggc
     3421 tgacggccgt ggcgccgaca ttccccgccg tgctcaccag cacatgcgcc gaacgcgcga
     3481 tggcggcata gtccgcgcgc tgagcggcat cggcaatggc cgccatgtgg ctgtccgcat
     3541 cggccagaaa catctccacg aaggcggtgg cctccgcaaa ggacaggacg gcatcgaggg
     3601 tttgcagctt ttcttggtcc agaacgccct cggaaccgcc gtcgggtgtc ggcggcgaaa
     3661 gctcgacggc ggccggaatt acccggggga gcctttcggc aatcgcggcg agcttggtga
     3721 gcagcaaggt cggctggacc ggtttggcga tataatcgtc catgccggcg gcgagatatt
     3781 cctcgcgcgc gcccttcatc gcatgggcgg tcatggcgat gatcgggatc gccgctttcg
     3841 gcatggacag tttgcggatc tgctgcgtcg cctggacgcc gtccagttcc ggcatctgga
     3901 tgtccatcag cacgacgtcg aaatcgtcgc gccgcagcgc atcgaccgcc ttgtgcccgt
     3961 tgtcgacgat ttcgacaaag tggcccgcct tctccagcag cgcgcgcgca tattgctggt
     4021 tgacgcggtt gtcctcggcg accaggacgc ggagctttcg cttcgccgcg gcggccgccg
     4081 ccttggaatc gcggcgatag accgcgacgg cggcgggttc gatgtggatg ctgtaaatgc
     4141 cgatcaggca atcgagcaga tcctgctggc gcaccggctt ctccaggatg gcatcgaact
     4201 tcacactcgt ctggctgcgg atggcctcgc ggccggccga ggagaccatc accagcttgc
     4261 actccgccag atgatcggat tgacgaatgc ggcgggccag ttcgctgccg gagattcccg
     4321 gcatcatctg gtccaagaag acgatgtcgt agggcttgcc gcggtgccat tcgcgttcga
     4381 gttcggcgat ggcggcgaaa ccgtcgtcca cgcccagaga ttccattccc agggcgtgca
     4441 actgccggcc caggatttca agattcatcg ccacatcgtc cacgatcagg gcgcgaagcg
     4501 ccttgaattg cgccgtcaga tcgtcgcggt cgacgaccgt cgtgccgatg cgggccagcg
     4561 ccagttcgaa ccagaacgtc gagccggcgc cgaggcggct cttcacgtcg atcctgccgc
     4621 ccatcagatc gataagctgc cggcaaatgg ccagccccag tcccgtgccg ccgtagcgcc
     4681 gggtcatcga attgtccacc tggctgaatt tctggaacag gcgctcgcgc acgctgtcgg
     4741 gcatgccgat tccggtgtcg gtgacttcga agcgcagcgg caccacgccg tcgggaagca
     4801 tggtctcgcc gagccgcaca ttcacctgga ccgagacccc gccccggtcg gtgaacttga
     4861 tcgcgttgct gagcaggttg agcagaatct ggcggatgcg cgtgggatcg cccatatagg
     4921 cgccgcgcgc cgccggctcg atgaagacgc ccagatcgat tcccttctcg cgcgccttcg
     4981 acgccatcag cttgacggcg ttctccaccg tcgacaccag atcgaactcg atccggtcga
     5041 tttcgaactt gccggcctcc agcttcgaga aatccaggac gtcgttgacg acggcgagca
     5101 gcgtatcccc ggattcctga atcgtttcgg caaatttgcg ctgctcttcg tcgaggtccg
     5161 ttcccaagag cagccccgcc atgccgagaa cgccgttcag cggggtgcgg atttcgtgac
     5221 tcatattggc gaggaactcg gacttggtgc ggttgccggc ttcggccagt tccttggcgg
     5281 cgatcaattg cccttccatg gcctcgtgcc gccgcatctg gcgcagcagg accagactca
     5341 gcgcaaccat cagaagcgta atggccgcca ggccgccgcc ttccaccaag gctccccgcc
     5401 gccactcttc aacggcgttg cctaccgggc gctgcgccac caccacatag cctgcggccg
     5461 gattggccgc gcgcgccacc agcatgtcgc cgttcacgcg cgggctcgct ttgctctcat
     5521 cgatgctgtc aaggtggagc gtcgttcccg aaggcagatt caaatcgctg cggaagcgcg
     5581 ccagttcccg cgcgccgtat tcgaggccgg cggacatcgg gttgcggtca gaagcgtcac
     5641 ccacagggat gctgatatct atagttgtca gcattcccgc atagtcggca taagcccgcg
     5701 cagcggtata taaattgcgc tgctcttcgg cgagcctgtc ggaatattcg gagacggtga
     5761 gccaagcgta gcccgaccac agcaaaacga tgaaggccag gatcagcgcg ccggcaatgg
     5821 cgctcggctg cagacctacg cgcaaccgcg acaagcgcgc gcgaaacgcg aaccgcatcg
     5881 acctcgacaa tttttcctgc tcttgctgcg ccaagcgctt tgtctgtggg agcgatacac
     5941 ggcatgctaa ggctgccggg cctgctacac gcaccaattg ttaccccgtc gcgctaatcc
     6001 gccgttaaga cggcaaaacg ctgggagagc atgcccaaag gcgcgcgcgc caaaaacccg
     6061 cccaatccgg ggaaaacaag gataagaaat attcattatc cggctgaaac ctgtggccgt
     6121 tttgccgccg gccgcatgga atgaaatcca caagcctttg tcttggcaca atcagggcct
     6181 atgaacagcg agtaacctcc ggagccccct ttatgcccag acttccaagc atgtccgctg
     6241 ccgccgttca aaagcgcaaa gaaatttcgg aggcggagaa agaagagagg gcaaaggcac
     6301 caccccccgc tccggtcgtc aaaatacgaa tcgcgaagcc gagatagact cgggcctaag
     6361 gcccaacgac ggctgccgac tctatgcgcg cggtaacagc ggcgcgtcct gccgggaaag
     6421 ttactggccc gtgtcgggtg gcgttgcgtc gctcgctggt ttcgtgcatc ccctgctctt
     6481 agaaaattca gacaatagca gaacaaatct cattttataa aataaattca atatattaac
     6541 tactatcttg tcattgggaa caaaacgtgt actttggtcc cggaccctgc ggcgctgaat
     6601 tcattgggaa tgcggcgcgc gccgtggaat aagggaggct ggcggtcatg aaagaagcgg
     6661 ttttgcgcgt cgtgggcaag gaagacggaa ccgacaagaa gcgtgcgctg gaagccgcgc
     6721 gttcgcagat cgatcgggcc ttcggcaagg gctcggtgat gaagctcggc cagcgcgacc
     6781 cggtgacgga agccgacacg gtgtccaccg gctcgctggg gctggacatc gcactcggca
     6841 tcggcgggct gccgcgtggc cgcgtaatcg aaatttacgg ccctgagtcc tcgggcaaga
     6901 ccactttggc gctgcatgcc gtcgccgagg tacagaagaa aggcggcatc gccgcttatg
     6961 tggacgccga acacgccctt gaccccggct atgccaagaa gctgggcgtg gacatcgacg
     7021 aactgctcat ctcgcagccc gacaccggcg aacaggccct cgaaatcacc gatacgctgg
     7081 tgcgttccgg cggcgtggac atcgtggtgg tggactcggt cgcggcgctc acgcccaagg
     7141 ccgaactgga aggcgaaatg ggcgatcaac tgcccggctt gcaggcgcgg ctgatgagcc
     7201 aggcgctgcg caagctgacc ggctcgattt ccaagtcgaa caccatcgtc atcttcatca
     7261 accagatccg catgaagatc ggcgtcatgt tcggcaatcc ggaaaccacc acgggcggca
     7321 atgcgctgaa attctattcc tcggttcgcc tggatatccg gcgcatcggc gccatcaagg
     7381 accatgacga agtggtgggc aaccagaccc gcgtgaaagt ggtcaagaac aaggtcgccc
     7441 cgccttttaa gcaggtcgag ttcgacatca tgtacggcca cggcatctcc aagtccggcg
     7501 agctgctgga cctcggcgtc aaggccggga cggtggagaa atcgggctcc tggttctctt
     7561 atgacggcac ccgaatcggc caggggcgcg agcaggccaa ggcttacctc gaagccaaca
     7621 aggacacggc ggccaagatc gaggccgcga ttcgccagaa cgcgggcctg atcggccaaa
     7681 gccttgccct gggcagcgtg gaggccgcgg aaggctgaac cttgtccgtt cccttgttgg
     7741 catgagggag cgactccaga aggggcgtgc cgggcgaccg gcgcgcccct tcaacatgga
     7801 agggatcggc ggcgaaagaa ccttaaaatt ccggtgtcat ggcctgcaaa tgcggccatc
     7861 caggcgacaa cggagtttgc actccggctg cgcttcgcgc ggaccggggt ggcgggccat
     7921 gacgccggtt atttgatccc gcgccaaact gtcctggtcc agatgacaag ctttttaacc
     7981 gccgctacaa ccttcccatg aaaagtctct ccgatatccg cgccggcttc ctggagttct
     8041 tccgcgaccg gcagcatgcc gtcgtgccct cctcgccgct ggtgccgcgc aacgacccca
     8101 cgctgctgtt caccaacgcg ggcatggttc agttcaagaa cgtgttcacc ggcgccgaaa
     8161 aacggcctta cagccgcgcc accagcgtgc agaaatgcgt gcgcgccggg ggcaagcaca
     8221 acgatctcga caatgtcggc tataccgccc gccatcacac ctttttcgag atgctgggga
     8281 acttctcctt cggcgactat ttcaaggaag acgcc
//
LOCUS       DASWPU010000223         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_112193,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000223 DASWPU010000000
VERSION     DASWPU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112193"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VGC27_04860"
     CDS             <1..546
                     /locus_tag="VGC27_04860"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY0281934.1"
                     /translation="QQQAGSEEDADQQAAAAPPAPSPALALRSQPVETKPVVQAPPTR
                     QTAAPKLAQVTQAAPPPAAPVDRPATQAPAKLLGATPATQAAAQSPVATPARAVAGGA
                     YLLQIGAFKSQSDADAAWNAYQAKHAALLSGFGPDVQRADLGDKGVWYRLRVGSFADK
                     DAAAAMCDRLKAEGAACFPVK"
     gene            577..>1522
                     /gene="nagZ"
                     /locus_tag="VGC27_04865"
     CDS             577..>1522
                     /gene="nagZ"
                     /locus_tag="VGC27_04865"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY0281935.1"
                     /translation="MRSRVIYGCSGQVLGAEERAFFRDAQPWGFILFARNVSNHEQVR
                     ALVDSLRETVADARAPVLIDQEGGRVARLTPPNWRERPAAARLGQAYAASPDVAAEAA
                     YLNARLIAADLGELGINVDCLPVLDLPVKGADNIIGDRAFASDPAATIALGRAVIEGL
                     LEGGVLPVMKHIPGHGRATADSHLALPRVATPAEELSATDFVTFRSLNQCPMAMTAHV
                     VYEDIDPQRPATTSPKVIRDVIRGEIGFDGLLLSDDLSMGALEGRISVRTKAALFAGC
                     DVVLHCNGKMEEMKDVAEGAKPLEGPALKRAEHALSLLS"
BASE COUNT          251 a          496 c          521 g          254 t
ORIGIN      
        1 caacagcagg ccgggtcgga ggaggatgcg gatcagcagg ctgccgctgc cccgccggct
       61 ccgtcgcccg cgctcgccct gcgctcgcag ccggtcgaga ccaagcctgt ggtgcaggcc
      121 ccgcctacgc gccaaacagc cgcccccaag cttgcccagg tgacgcaagc ggcgcctccg
      181 cctgccgcgc cggttgatag accggccacc caggcccctg cgaagctgct cggtgcgaca
      241 ccggccaccc aggctgctgc gcaatcgccc gtcgcgacgc cggcaagggc cgtggccggc
      301 ggggcctatc tgctgcaaat cggcgcattt aaatcgcaaa gcgatgccga tgccgcctgg
      361 aatgcctatc aggccaagca tgcggcgctg ctttccggct tcgggcctga cgttcaaagg
      421 gccgatcttg gcgacaaggg cgtgtggtat cgcctgcgcg tggggtcgtt cgctgataaa
      481 gatgccgcag ccgcgatgtg cgaccggctg aaagcggaag gtgccgcatg tttcccggtg
      541 aaatgatgtg agcgtcgagt acgggtaaaa gctaccatgc ggtcacgggt catttacggc
      601 tgctccggcc aggtgctggg agcggaggag cgcgcgttct tccgcgacgc gcagccatgg
      661 ggtttcatcc tttttgcccg caacgtttcc aaccacgaac aggtgcgtgc gctggtcgac
      721 tctctgcgtg agaccgtcgc cgatgcgcgc gcgcccgtgc tgatcgatca ggaaggcggg
      781 cgcgtggcgc ggttgacccc gcccaactgg cgcgagcgtc cggccgccgc acggctcgga
      841 caggcttacg cggcttcgcc cgacgtggcg gcggaagcgg cctatctcaa cgccagattg
      901 atcgccgccg atctcggcga acttggcatc aatgtcgatt gtctgccggt gctggacctg
      961 ccggtgaaag gcgccgacaa cattatcggc gaccgcgcgt tcgcgtccga tcccgccgca
     1021 accatcgcgc tggggcgcgc cgtcatcgaa gggctgctgg aaggcggcgt gctgccggtg
     1081 atgaagcata tccccggcca cgggcgcgcg acggcggatt cccatctggc gctgccgcgt
     1141 gtcgctacgc cggccgagga actgagcgcc accgatttcg tcaccttccg cagcctgaac
     1201 cagtgcccga tggcgatgac ggcgcatgtc gtctatgaag acatcgatcc gcagcgcccg
     1261 gcgaccacca gcccgaaagt gattcgcgat gtgattcgcg gcgagatcgg cttcgacggg
     1321 cttttgctgt ccgacgacct gtcgatgggg gcgctggaag ggcggatttc cgtgcgcacc
     1381 aaggcggccc tgttcgccgg ttgcgatgtc gtgcttcact gcaacggcaa gatggaagag
     1441 atgaaagacg tggcggaagg ggccaaaccg cttgaaggcc cggcgctgaa acgtgcggag
     1501 catgcgctgt cgctgttgag cg
//
LOCUS       DASWPU010000224         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_106069,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000224 DASWPU010000000
VERSION     DASWPU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106069"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VGC27_04870"
     CDS             <1..370
                     /locus_tag="VGC27_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281936.1"
                     /translation="IGGRKERIDGDLAAASKHRKDAEAALATYQSALAEANARAQKAA
                     DENRKLVADEVEKARTAAEAEAKEAMAQAETRIAAVRQEAAKHVTIAAQDAAASIVAR
                     LIGVTISPEEAAAAVKASGP"
     gene            390..974
                     /locus_tag="VGC27_04875"
     CDS             390..974
                     /locus_tag="VGC27_04875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF017242.4"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HEY0281937.1"
                     /translation="MSAFRLATASLAIGVAGFVLVIHRAEVPGITMTLFQNPEFWVAV
                     GFAIVVAIFLRVKVPAMLAKLLDARAAAIAKELDEAKRLREEAAALLAGYVQKVARAE
                     SEAAAILADAKAEAERFAKETRAQLRAQVDRRAQMAKDKIEQAEAAALIEIRALAADT
                     AAAAAEKLIAARLDEKRSAALVEESIKELPDKLN"
     gene            996..1526
                     /locus_tag="VGC27_04880"
     CDS             996..1526
                     /locus_tag="VGC27_04880"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY0281938.1"
                     /translation="MALLRGINVGGHKMVAMADLRALTTNLGFEDVQTVLQSGNLVFS
                     GATRTTASLEALLEKETAKRLGLDTSFMIRTAAEWTKIVAGNPLRDEAKNDPGRLAVI
                     FFKDKPAKKNLDALRGCISGREYFEASGRELYAVYPDGQGNSKFTLSLIDRKLETRGT
                     GRNWNTVLKLAKLVEA"
     gene            complement(1717..>3098)
                     /gene="aspS"
                     /locus_tag="VGC27_04885"
     CDS             complement(1717..>3098)
                     /gene="aspS"
                     /locus_tag="VGC27_04885"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY0281939.1"
                     /translation="TLRSKVISSIRRRMIEQGFTEFQTPILTASSPEGARDFLVPSRL
                     HPGKFYALPQAPQQFKQLIMVAGFDRYFQIAPCFRDEDARADRSPGEFYQLDLEMSFA
                     TQDDVFAAVEPVLHGVFEEFAEGRSVSPAPFPRIPYDESMLKYGVDKPDLRNPIVIAD
                     VSSVFARQDVAFKAFKDKTVRAIPAPGAGAQPRSWFDRMDAWARSDMGAKGLGYIVFE
                     ETSGSLTGKGPIAKFFSPEALAELAKVAGIKAGDALFFSADAKKDRAAALAGAARVRL
                     GRELGLIREGEFRFCWIVDFPMYEWNEAEKKIDFSHNPFSMPQYGREKFLALDGADHD
                     TILGLKAYQYDIVCNGVELSSGAIRNHDPDVMLKAFEIAGYAREETEIKFAGMLNAFR
                     YGAPPHGGTAPGIDRIVMLIAGVENLREVTMFPMNQQAQDLMMNAPSEASLKQLRDLH
                     IRVVLPEKS"
BASE COUNT          614 a          979 c          973 g          532 t
ORIGIN      
        1 gatcggcggc cgcaaggaac ggatcgacgg cgatctggcc gcggcgtcca agcaccgcaa
       61 ggacgcggag gcggcgctgg ccacctacca gtccgccctc gccgaggcca acgcccgcgc
      121 ccagaaagcg gccgacgaga accgcaagct ggtcgcggac gaagttgaaa aagccagaac
      181 tgcggcggaa gccgaggcca aggaggccat ggcccaggcc gaaacccgca tcgccgccgt
      241 gcgtcaagaa gctgcaaaac atgtcacaat tgcggcacaa gatgccgccg cttctatcgt
      301 cgcgcggctg atcggggtta caatctcccc cgaggaggct gccgcggccg tgaaggcgtc
      361 gggaccctga cgatggggag ggaaacgtca tgtcggcttt tcgccttgcg acggcgtcgc
      421 tcgcgatcgg cgtggcgggt ttcgttcttg tcatacatcg cgctgaggta ccgggaatca
      481 cgatgacgct tttccaaaat cccgagttct gggtagccgt cggtttcgcc atcgtggtgg
      541 cgatcttcct gcgcgtgaaa gtgccggcga tgctggccaa gctgctggat gcgcgcgccg
      601 ccgccatcgc caaggaactc gacgaagcca agcggctgcg cgaggaagcc gccgccctgc
      661 tcgccggcta tgtgcagaag gtcgcgcggg ccgaaagcga agccgccgcc atcctcgccg
      721 acgccaaggc cgaagcggaa cgctttgcca aggaaacccg cgcccagctg cgcgcccagg
      781 tcgatcgccg cgcccagatg gcgaaggaca agatcgaaca ggccgaggcc gcggcgctga
      841 tcgaaatccg cgcgcttgcc gccgatacgg ccgccgccgc cgccgaaaag ctgatcgccg
      901 cgcgcctcga cgaaaagcgc tccgccgcgc tggttgaaga gagcatcaag gaactgccgg
      961 acaagctgaa ctagggtaag cccacggcca cttacatcgc gctccttcgc ggcatcaacg
     1021 tgggcggcca caaaatggtc gcgatggccg atttgcgcgc gctgacaacg aacctcggct
     1081 tcgaagatgt gcagaccgtg ctgcaatccg gcaatctggt tttcagcggc gcaacgcgaa
     1141 cgaccgcttc actggaagcg ttgttagaaa aggaaacggc aaagcgcctc ggcctcgaca
     1201 ccagtttcat gatccgcaca gcggctgagt ggacgaagat cgtcgctggc aatcctctcc
     1261 gcgacgaagc gaaaaatgat cccggccgcc tcgccgtgat cttcttcaaa gacaaaccag
     1321 ccaagaaaaa cctggacgcc ctgaggggtt gcatcagcgg tcgggagtat tttgaggcga
     1381 gcggccgtga actttatgcc gtctatcccg acggccaggg taattcgaaa ttcaccctgt
     1441 ccttgatcga ccggaagctg gaaacacgcg gcaccggcag gaattggaac accgtgctga
     1501 agctggcaaa gctggtggaa gcgtaacagc gttccagttt tgcctccccc ttcaagggga
     1561 aggtgaaatt ataccaatgt gtgtggttct taaccctttt cgtcatggcc gggcttgacc
     1621 cggccatcca gtttctcctt gaaactccgt gcttttctgg atggccgcct caagggcggc
     1681 catgacggaa tgagtcagtg atcatgccgg ttggtattag gacttctccg gcaacaccac
     1741 ccggatgtgc aggtcgcgca actgcttcaa cgaggcctcg ctgggcgcgt tcatcatcag
     1801 gtcctgggcc tgctggttca tcggaaacat ggtgacttcg cgcagattct cgacccccgc
     1861 gatcagcatg acgatgcggt cgatgcccgg cgcggtgccg ccatgcggcg gggcgccgta
     1921 acggaacgcg ttcagcatcc cggcgaactt gatttcggtc tcttctcgcg cgtagcccgc
     1981 gatctcgaac gccttcagca tcacgtcggg atcgtggttg cggatggcgc cactcgacag
     2041 ttcgacgccg ttgcagacga tgtcgtactg ataggccttg aggccgagga tggtgtcatg
     2101 atccgcccca tcgagcgcca ggaacttctc gcggccatat tgcggcatgg agaaggggtt
     2161 gtgcgagaag tcgatcttct tctcggcttc attccactca tacatcggaa aatcgacgat
     2221 ccagcagaat ctgaactcgc cctctctgat caacccaagc tcgcggccga ggcgcacccg
     2281 cgccgcgccc gccagcgccg ccgcgcggtc cttcttggcg tcggccgaaa agaacagcgc
     2341 gtctcccgcc ttgatgcctg ccaccttggc gagttccgcc agcgcctcgg gcgaaaagaa
     2401 tttggcgata ggccctttgc ctgtcagcga cccactcgtt tcttcgaaca cgatgtagcc
     2461 gaggcctttc gcgcccatgt cggagcgcgc ccaggcgtcc atccggtcga accacgagcg
     2521 cggctgcgca cccgcccccg gcgccggaat ggcgcgcacg gttttgtcct tgaacgcctt
     2581 gaaggccacg tcctggcgcg cgaagacgct ggaaacatcg gcgatgacga tggggttgcg
     2641 caggtccggc ttgtcgacgc cgtatttcag catcgactcg tcatagggaa tgcgcgggaa
     2701 cggcgcgggc gacacgctgc ggccctccgc gaactcttcg aacacgccat gcagaaccgg
     2761 ctcgacggca gcaaaaacat cgtcttgcgt ggcgaagctc atttccaggt cgagctgata
     2821 gaactcgccc ggcgagcggt cggcgcgggc atcctcgtcg cggaaacaag gcgcgatctg
     2881 gaaatagcgg tcgaagcccg ccaccatgat gagctgcttg aactgctgcg gcgcctgggg
     2941 cagcgcatag aacttgcccg gatgcagccg cgaaggcacc aggaagtcgc gcgcgccttc
     3001 cggcgaggag gccgtcagga tcggggtctg gaattcggtg aagccctgct cgatcatccg
     3061 gcggcggatc gacgagatca ccttggaacg cagggtga
//
LOCUS       DASWPU010000225         4463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_1717, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000225 DASWPU010000000
VERSION     DASWPU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..704)
                     /locus_tag="VGC27_04890"
     CDS             complement(96..704)
                     /locus_tag="VGC27_04890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0281940.1"
                     /translation="MKPGPMAFMRGLTFPGGQHPVIRGKDVYLRYPRIADFKVWARLR
                     GESRKFLEPWEPSWAGDELTKGAYRRRIKRYQKETRQDSAYAFFVFRSEDDALMGGCT
                     LSNVRRGVTQCCALGYWIGERFARQGYMFDAVRGLIPFIFATLGLHRIEAACLPSNEP
                     SKNLLMKAGFRQEGFALRYLQINGEWRDHILFALLADEAQIV"
     gene            complement(701..1990)
                     /locus_tag="VGC27_04895"
     CDS             complement(701..1990)
                     /locus_tag="VGC27_04895"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0281941.1"
                     /translation="MNIKISRLSNGLVVITDPMPQLESAAVGVWVSCGARNETAPLMG
                     VSHMLEHMAFKGTRRRSARDISFEIESVGGFLNAYTSREQTAFHARALKADVPLALDI
                     LADILTEPTFDPVELGRERQVVLQEIGQARDTPDDIIFDYLQTVVYPDQPMGWPILGE
                     EETVAAFGRDDLFTYMGANYRAGGMTLVASGAVDHDEIVRLAEEKFSRLVPGEPQAPP
                     PARYAGGEGHYAGDLEQAHIAFAFPGVSALDPDYYVAQVYVAALGGGMSSRLFQEVRE
                     KRGLCYSIYAFAHSAKDSGVVGIYAGTGEAEAAEIGAVVAGEMAALAETASEEEIARA
                     KAQLRSGLLMGLESPGTRAEMIAGHIFAHGRLVSIEELTAKLDAVDAAAIRRYGARTM
                     TATEPPAIVTVGPAGKLEPYEVFAGRFGSRQIVRAAE"
     gene            complement(1987..3429)
                     /gene="thrC"
                     /locus_tag="VGC27_04900"
     CDS             complement(1987..3429)
                     /gene="thrC"
                     /locus_tag="VGC27_04900"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY0281942.1"
                     /translation="MRYLSTRGQSPLQSFADMLLAGLAPDGGLYLPEAWPRFSPAEIA
                     DFASQPYADVAGAVIGRLVGDSFSNEELAADIAAAYAGFDDKRIAPLVEIGQDLYLLE
                     LFHGPTLAFKDVALQVLGRLFERALKKRGGRTPRGGRTPRGGRTPRGGRATVLAATSG
                     DTGSAAIAALGGLANIEVFVLHPKGRVSEVQRRQMTAAPFENVHNIALEGSFDDAQAI
                     VKALFAETEFAQAVNLTAVNSINFARIAAQSVYYFTAAAQLGAPPVFVVPTGNFGDIF
                     AGEAAMRMGLAVEKLVVATNANDILARALNEGVYAAGRAQPTLSPSMDIQVASNFERA
                     LFEASCRNALWLREAMAQFAGAKSIALPDGVRIALRARYAAKRCDDAETLAAIARVHA
                     ETGRLIDPHTGVGVAAAYNLGEHRKGPVVVLSTAHPAKFPDAVERATGRIPPVPPRLS
                     EALKATEKMVVLAADKGLVRAFIEAKVPPP"
     gene            complement(3744..>4463)
                     /locus_tag="VGC27_04905"
     CDS             complement(3744..>4463)
                     /locus_tag="VGC27_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY0281943.1"
                     /translation="VSTMPYFRPLPILTFACAIAFAILISLGVWQLERLHWKLGLIAQ
                     VNNSLTATPVSLDQALAMGAAAQYHRVLLDGWFFNDKESYLYASGPDGAPVYHVLTPF
                     ETREGRVLMVDRGIVPAELIDPAKRARGQIGERVMIGVWRIPDKPGFFTPRPDTAHRI
                     WYSRDVAAMAAAVQVKLTAPVLVEADATPNPGGWPKGGQTQVTFRNEHLQYALTWFAL
                     AATLLGVYLAYHISKGRLRWR"
BASE COUNT          820 a         1434 c         1453 g          756 t
ORIGIN      
        1 gacactagca acctgttgaa tcctagtgtc gttttggttt cgaagttcgc ttgcagcccg
       61 atagtgcgca tgctgcgaac ttcgaaacca cgacactaga ctatctgcgc ctcatccgcg
      121 agcaaagcaa ataggatgtg atcgcgccac tcgccgttga tctgcaggta acgaagcgcg
      181 aagccctctt ggcggaatcc cgccttcatc aacaggttct tggaaggttc gttcgacggc
      241 aggcaagccg cctcgatccg atgcagaccc agagttgcga agatgaacgg gatcagtccc
      301 cttaccgcgt cgaacatata gccctggcgt gcaaaacgtt cgcctatcca gtagcccaga
      361 gcacaacatt gcgtgacgcc acgccgcaca ttggagagcg tgcagccgcc catcagcgca
      421 tcgtcttcgc tgcggaaaac gaaaaaggcg taggccgaat cctgacgtgt ctctttctgg
      481 taacgcttga tgcgccggcg atacgcccct ttcgtcaatt cgtcgccggc ccaggacggc
      541 tcccagggtt cgaggaattt cctgctctcg ccgcgcaagc gcgcccagac cttgaaatcg
      601 gcgatgcgcg ggtaacgcag atatacgtcc ttgccgcgta tcaccgggtg ctgaccgccg
      661 ggaaacgtca gcccgcgcat gaaggccatg ggacccggtt tcattcggcg gcgcgcacga
      721 tttgccgcga accgaaacgc cccgcaaaga cctcataggg ctcgagcttg ccggcgggac
      781 cgacggtgac gatcgctggc ggttctgtcg ctgtcatcgt gcgggcgcca tagcggcgta
      841 tggcggcggc gtcaaccgca tcgagcttgg cggtcagttc ctcgatcgaa accagccggc
      901 cgtgggcgaa gatgtgacct gctatcattt cagcgcgcgt gccgggactc tccaagccca
      961 tcagcagacc cgagcgcaac tgcgccttgg cgcgcgcgat ttcctcttcg ctcgcggtct
     1021 cggcaagcgc cgccatttcg ccggccacga ccgcgccgat ctcagccgcc tcggcctcgc
     1081 ccgtgccggc atagatgccg accacgccgc tgtccttggc gctgtgcgcg aaggcgtaga
     1141 tcgaatagca gagcccgcgc ttctcgcgca cctcctggaa cagccgcgaa gacatgccgc
     1201 cgccgagcgc ggcgacgtaa acctgcgcga cgtaatagtc gggatccagc gccgacacgc
     1261 cgggaaaggc aaaagcgatg tgcgcctgtt ccagatcgcc cgcataatgc ccttcgccgc
     1321 cggcatagcg cgcgggcggc ggcgcttgcg gttcgccggg aaccaggcgg gaaaatttct
     1381 cttccgccaa ccgcacgatc tcgtcgtgat cgacggcgcc ggaagccacc agcgtcatgc
     1441 cgccggcgcg gtaattggcg cccatatagg tgaacagatc gtcgcgcccg aaggcggcga
     1501 cggtttcctc ctcgcccaga atcggccagc ccatcggctg atcgggatag acgacggttt
     1561 gcaggtaatc gaagatgatg tcgtcgggcg tatcgcgggc ctggccgatc tcctgcagca
     1621 ccacctggcg ttcgcggccg agctcgaccg gatcgaaagt gggttcggta aggatgtcgg
     1681 cgaggatgtc gagcgccagc ggcacatcgg ccttcaaggc gcgcgcgtgg aacgccgtct
     1741 gctcgcggct ggtgtaggcg ttgagaaagc cgccgaccga ttcgatctcg aaggagatgt
     1801 cgcgcgcgct gcgccgccgc gtgcctttga acgccatatg ttcaagcatg tgcgagacgc
     1861 ccatcagcgg cgccgtttcg ttgcgcgcgc cgcaactcac ccacacgccg acggcggcgc
     1921 tctcgagctg aggcattgga tcggtgatga caaccaaccc attggaaaga cgactgattt
     1981 ttatgttcat ggtggaggca ctttagcctc gatgaaggcc cgaaccaagc ccttgtccgc
     2041 ggctaaaacg accatttttt cggtggcctt aagcgcctcg ctaagcctcg ggggcaccgg
     2101 gggaatccgg cccgtcgccc gctccaccgc atccgggaac ttggccggat gggcggtcga
     2161 caggacaaca accggacctt tccgatgctc gcccaggttg taagccgccg ccacgccgac
     2221 cccggtatgc ggatcgatca ggcggccggt ttcggcgtgg acgcgggcga tggcggcaag
     2281 cgtctcagcg tcgtcgcagc gcttggcggc atagcgcgcg cgcaaggcga tgcgaacgcc
     2341 gtcgggaagc gcgatgctct tggccccggc aaactgcgcc atcgcctcgc gcagccatag
     2401 ggcgttgcgg cacgaggcct cgaacagcgc ccgctcgaaa ttgctggcga cctgaatgtc
     2461 catcgacggg ctgagcgtcg gctgcgccct gcccgcggca tagacccctt cgttgagcgc
     2521 gcgcgcgaga atgtcgttgg cattggtggc gacgacgagc ttttcaaccg ccagccccat
     2581 gcgcatggcg gcctcgccgg cgaagatgtc gccgaaattg ccggtgggca cgacgaagac
     2641 cggcggcgcg ccaagctgcg ccgccgcggt gaagtaatag acgctctgcg ccgcgatgcg
     2701 cgcgaagttg atggaattga ccgcagtgag gttcaccgcc tgggcgaatt ccgtctccgc
     2761 gaacagcgcc ttcacgatgg cctgggcgtc gtcgaaactg ccttccagcg cgatgttatg
     2821 gacgttctcg aacggcgccg cggtcatctg gcggcgctgc acctcgctga cgcggccttt
     2881 cggatgcagc acgaacactt cgatgttggc cagcccgccg agcgcggcga tggccgcgga
     2941 gccggtatcg ccggaggtgg ccgcgagaac ggtggcgcgc ccaccccggg gggtgcgccc
     3001 accccggggg gtgcgcccgc cccggggggt gcgcccaccc cgcttcttca gtgcgcgctc
     3061 gaacaggcgg ccgagcacct gaagcgcgac atccttgaag gcgagcgtag ggccgtggaa
     3121 cagttccagc agataaaggt cctggccgat ctcgaccagc ggcgcgatgc gcttatcgtc
     3181 gaagccggca taggcggcgg cgatgtcggc ggcgagttct tcgttcgaga acgagtcgcc
     3241 aaccaagcgg ccgatgacgg cgcccgcaac gtcggcataa ggctgcgagg cgaaatcggc
     3301 gatctcggcg ggcgaaaagc gcggccacgc ctcgggcagg taaagcccgc cgtcgggcgc
     3361 gaggcctgcc agcagcatgt cggcgaagga ttgcagcggg ctttggccgc gagtgctcag
     3421 atagcgcatg gttcagcaat ataattgtta cgagattgga tcagggggca ttctagacaa
     3481 agttcgcccg cccaccataa cggggttttt tgaaagtgcg cacccttcga tgctccgccc
     3541 tcacggcggc cgtccagaaa tgaagcacgc ggaggcgcgg agccgctctt ctactccgcg
     3601 ttctccgtgt ctccgcgtga caatctggat ggccgggtct tgcccggcca tgacgaagca
     3661 agagggcgca acgggttgga tatagggaaa atggcaatcc gaccggcacg gttcaagcag
     3721 tggtactgtc ccatttaaag gtgtcaccgc cacctcagcc ttcctttgct gatgtgataa
     3781 gcgaggtaga cgcccagcag ggtcgcggcg agggcgaacc aggtgagcgc gtattgcaga
     3841 tgctcgttgc ggaacgtcac ctgcgtttgg ccgcccttgg gccagccgcc gggattgggc
     3901 gtggcgtcgg cttccaccag cacgggcgcg gtgagcttca cctgaacggc ggcggccatc
     3961 gcggccacgt cgcgcgaata ccagatgcga tgcgccgtgt cgggccgcgg cgtgaaaaac
     4021 cccggcttat cggggatgcg ccagacgccg atcatcaccc gctcgccgat ctgcccgcgc
     4081 gcccgcttcg ccggatcgat cagttcggcc ggcacgatgc cgcgatccac catcagcacc
     4141 cgcccttcgc gcgtctcgaa cggcgtcagc acatgataga ccggcgcgcc atcggggccg
     4201 ctggcgtaga ggtaggattc cttgtcgttg aagaaccagc cgtcgagcaa cacgcgatgg
     4261 tattgcgcgg cggcgcccat cgccaaagcc tggtcgagcg agaccggcgt cgccgtgagg
     4321 ctgttattga cctgcgcgat caggccgagc ttccagtgca ggcgctcaag ctgccagacg
     4381 ccgagcgaga taaggatcgc gaacgctatc gcacaggcga aagtgagaat gggaagcggg
     4441 cggaaatagg gcatggtgct cac
//
LOCUS       DASWPU010000226         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_69452,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000226 DASWPU010000000
VERSION     DASWPU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69452"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /gene="rpsB"
                     /locus_tag="VGC27_04910"
     CDS             complement(<1..576)
                     /gene="rpsB"
                     /locus_tag="VGC27_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY0281944.1"
                     /translation="MALPDFTMRQLLESGAHFGHRTQRWNPKMAPFIYGSRNDIHIID
                     LTQTVPLLHQALVALRDVAAGGGRILFVGTKRQASEPIAAAAKRCAQYYVNHRWLGGT
                     LTNWQTISNSIKRLRGIEETLDSGQGSGLTKKELLGLFRERDKLERALGGIKEMGGLP
                     NMLFVVDTNKEAIAISEAKKLGIPVTAILDSN"
     gene            927..1160
                     /locus_tag="VGC27_04915"
     CDS             927..1160
                     /locus_tag="VGC27_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281945.1"
                     /translation="MATPAFIREAAKRAQRVGVAFHNYARGNAANGSYARICFAQDTE
                     RLSESFHAWRELRNHDASFQLYITRTQRVYTRM"
     gene            1240..>3211
                     /locus_tag="VGC27_04920"
     CDS             1240..>3211
                     /locus_tag="VGC27_04920"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HEY0281946.1"
                     /translation="MHTLRFVACIIVAVSALGASGSAARAQTPSARDFAAYRPTAQAT
                     RIDASQAPVIDGDISDPVWSKAQPIDELYQLEPNEGAAASERTVVRILHDENNLYFAI
                     MAFDTEPGRITARIKQRDASIDRDDIIRIYLDPDMTRRNAFIFEVNPLGARRDGLIQN
                     NSTMLYEWNTMWSAEARILPTGWSVEVAIPFRSISYGKRSDWGFDFYRLVRRKNERIR
                     WSSINTTIATQDVTQSGTLTGIGGIHEGLGLDVQAFALARYTKVWDAPGDGSGLSVRP
                     SGNIFYKVTPSLTGTLTYNTDFSDAPLDQRKVNISRFSLFYPERRDFFLQDAAAFEFG
                     GLALGVNQDPNAAPFISRRIGIVNNEAVNILGGAKISGQHDGFGIGALSVLTAGGAGA
                     GEQLLSTARVTMPVLSEAKLGLIVTNGDPSGLSSNTLAGGDFQFHDSAFFGNKIVQGD
                     FYYERSFSSVAGQDDSFGVNLNFPNEPWNGYFRFKQVGENFDPALGFVSRPGIRDYQG
                     DIVHRQRFSDSAIRWWDVGLWTEFTTGLNNEIQSRYNYDVWTSAYTEAGDYFQVEAWE
                     DYENVPSFSLPRGIAVPAGEYTFSVLRLRTETAPGRFLSGVVDVQAGGFYGGRLLQTD
                     TTVNLNPNDTYTFSARHIMQQISLPAGKVAIHV"
BASE COUNT          603 a         1049 c          977 g          582 t
ORIGIN      
        1 gttcgaatcc aggatggccg tcaccgggat gccgagcttc ttggcttccg aaatggcgat
       61 ggcttccttg ttggtatcga ccacgaacag catgttgggc aggccgccca tttccttgat
      121 gccgcccagc gcgcgttcga gcttgtcgcg ttcgcggaac aggccgagca gttccttctt
      181 ggtcaggccc gagccctggc cggaatccag cgtctcttcg atgccgcgca gccgcttgat
      241 cgaattggag atggtctgcc agttggtcag cgtgccgccc agccagcgat gattgacgta
      301 atactgggcg cagcgcttgg ccgccgccgc gatcggctcg gatgcctgcc gcttggtgcc
      361 gacgaaaagg atacgcccgc cgcccgcggc gacgtcgcgc agcgccacca gcgcctgatg
      421 cagcaaaggc acggtctggg tcaggtcgat gatgtggatg tcgttgcgcg agccatagat
      481 gaagggggcc attttcgggt tccagcgctg ggtgcgatgg ccgaaatggg cgcccgactc
      541 gaggagctgg cgcatggtga aatctggcaa tgccatgaaa tacttctcct tttccggtta
      601 gccaagcgcg aggagtaacc ccttttgtct ggtcgctccc gctctcgcgg acgctccagg
      661 ggcaccggac ggggacacag gaacaccctg cataacccac cctcgcgcgc ggaatggtgc
      721 gggttatagg gggtggcccg ggcgagcgca agctgaccgt ccgcgcggcg gcgcgccctt
      781 tcctcagccc gtcgcggccg ggaatattct gcctcgatgc catgacgccc gcccatcctg
      841 gcactgaagc ccaacgggat cggccgcatg tcgtccccgg gttgaggcag acggacatca
      901 tccctttgcg gtttggggaa agcgacgtgg cgacgcctgc cttcatccgc gaagcagcca
      961 aacgcgcgca gcgcgtcggc gtcgccttcc acaactacgc gcgcggcaat gcggccaacg
     1021 gcagctatgc ccgcatctgc tttgcccaag acaccgaacg gttgagcgaa agctttcacg
     1081 catggcgcga acttcgaaac cacgacgcga gtttccagtt atacattacg cgcacacaaa
     1141 gggtgtacac gcggatgtga cgatgcgtgc caggtgcccg ccgcgaaaca accgcaattc
     1201 atgcgaacat ttcggccgcg gaatttaggg gcgccgtcca tgcacacgtt gcgtttcgtc
     1261 gcgtgcatta tcgtggccgt atcggcgctg ggggcaagcg ggagcgccgc acgggcccaa
     1321 acgccctccg cccgcgattt cgccgcctac cggccgaccg cccaggcgac gcgcatcgac
     1381 gcttcgcagg cgcccgtcat cgacggcgac atctccgatc ccgtctggag caaggcgcag
     1441 ccgatcgacg agttgtacca gctcgaaccg aacgaaggcg cggcggccag cgagcgtacg
     1501 gtcgtgcgca ttctccacga cgaaaacaat ctctatttcg cgatcatggc gttcgacacc
     1561 gagcccggcc ggatcaccgc ccgcatcaag cagcgcgacg ccagcatcga cagagacgat
     1621 atcattcgga tttatctcga ccccgacatg acgcgccgca atgccttcat cttcgaggtc
     1681 aatccgctgg gcgcccgccg cgacggcctg attcaaaaca acagcaccat gctttatgaa
     1741 tggaacacga tgtggagcgc cgaagcccgc atcctgccca ccggctggtc ggtggaagtc
     1801 gccatcccct tccgcagcat ctcctacggc aagcgcagcg actggggttt cgatttctac
     1861 cgcctcgtcc gccgcaagaa cgaacgcatc cgctggtcgt cgatcaacac gaccatcgcg
     1921 acccaagacg tcacccaatc cggcaccctc accggcatcg gcggaatcca cgaagggctg
     1981 ggactcgacg tgcaggcctt cgccttggcg cgctatacga aagtctggga cgcccccggc
     2041 gacggcagcg gcctcagcgt caggccgagc gggaacatct tctataaggt cacgccctcg
     2101 ctgacaggca cactcaccta caacaccgat ttctcggatg cgccgctgga ccagcggaaa
     2161 gtgaacatct cgcgcttcag cctgttctat ccggaacggc gcgacttctt cctgcaggac
     2221 gcggcggcgt tcgaattcgg agggctcgct ctcggcgtca accaggaccc caatgcggcg
     2281 ccgttcatct cgcggcggat cggcatcgtc aacaacgagg cggtgaacat tctgggcggt
     2341 gcgaaaatct cgggccaaca tgacgggttc ggcatcggcg cgctcagcgt tctcacggcg
     2401 ggcggcgccg gggccggcga acaattgctc tcgacggcgc gcgtcacgat gccggtgctg
     2461 agcgaagcca agctggggct tatcgtcacc aacggcgatc cgtccgggct ttcgagcaac
     2521 accctggcgg gcggcgattt tcagttccac gactccgcct ttttcgggaa caaaatcgtc
     2581 cagggcgatt tctattacga gcgcagcttc agcagcgttg cgggccagga cgattccttc
     2641 ggcgtgaatc tgaatttccc caacgaacct tggaacggct atttccgctt caagcaggtg
     2701 ggagagaatt tcgatccggc gctcggcttc gtttcgcgcc cgggcatccg cgattaccag
     2761 ggcgacatcg tgcatcgcca gcgtttcagc gattccgcca tccgctggtg ggacgtgggc
     2821 ctctggacgg aatttacgac ggggctgaac aacgagattc agtctcgcta taattacgac
     2881 gtctggacaa gcgcctatac ggaagcgggc gattacttcc aggttgaagc atgggaggac
     2941 tatgagaacg tgccgtcgtt ctcgctgccg cgcggcatcg ctgttccggc gggtgaatac
     3001 actttcagcg tgctgcgtct gcggaccgaa acggcgccgg gccgcttcct ctcgggcgtc
     3061 gtcgacgtgc aagccggcgg cttctacggc ggccggctgc tgcaaaccga caccaccgtg
     3121 aacctgaatc cgaacgacac ctataccttc agcgcacgcc acatcatgca gcagatttcc
     3181 ttgcccgccg gcaaggtcgc gattcacgtc g
//
LOCUS       DASWPU010000227         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_81429,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000227 DASWPU010000000
VERSION     DASWPU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81429"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VGC27_04925"
     CDS             <1..1119
                     /locus_tag="VGC27_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281947.1"
                     /translation="GWTGVKMGAQRPLTFGPDEMTRLKQIVLGGEAKACAGGSYAFVE
                     NFRERYLAAVTAGHRLSRPIKVVAACGNGTAGAFAPEALRRIGAEVIEMDATLDYTFP
                     KYNPNPEDMQMLHAMADAVRAHKAELCMGFDGDGDRCGVVDDKGEEIFADKVGVLLAR
                     DLSSRIPNAQFVVDVKSTGIYLMDPVLKQNGVRVDYWKTGHSYIKRRTAELKAVAGLE
                     KSGHYFFNPPLGRGYDDGILAAIAVLEMLDHAGGKLSALVAGLPKTWSSPTMSPFCSD
                     ESKYNVVAAITQEYEGIKARGEKVLGQKIVSIVTVNGVRVTLEDGTWCLVRASSNKPS
                     LVVVVESPSSEANMHAIFNDLDGRLSKHPDVGAYDQKI"
     gene            complement(1250..2086)
                     /locus_tag="VGC27_04930"
     CDS             complement(1250..2086)
                     /locus_tag="VGC27_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0281948.1"
                     /translation="MDYTTFAATGDIGTLALGLLIAGVVSGLIAGMLGVGGGIVVVPV
                     MYHVLVAMNVSEDLRMHIAVGTSLATIIPTSISSLRAHDKKGAVDRELLKRWLWPMLA
                     GVLAGGVLAGVSRGQTLALIFGLVALPVAAQLAFMKETWRLADKLPGGIGGALLPFGI
                     SGISTMMGIGGGTVGVPAMTLCGVPIHRAVGTASAFGAIISIPGAIGAAIAGYGAAGL
                     PPYSLGYVNLLGFLLIAPVAFFTAPAGAAIAHMTDRGRLRRLFALFVAITAAKMLWDA
                     LA"
     gene            2178..3185
                     /locus_tag="VGC27_04935"
     CDS             2178..3185
                     /locus_tag="VGC27_04935"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0281949.1"
                     /translation="MDRRTVLGLGAATLAGLSVRAFAEEMNAEGVLSTDPTEIVPLWP
                     GTPPGGEGVKIKAKIVERSPDTNAYHDRFVSDIGMPWLSVFRPERPDGSAVLLAPGGG
                     YTRIVIDKEGVEAGRRFNASGVTVFVLYYRLPAEGWANGKDVPLQDAQRAMRLIRAGA
                     QGYGIDPDRVGVMGFSAGGHVAASLATRQNEPVYAPVDAADRKDARPAFAALMYPVIT
                     MEPGRHQGSRDRLLGAEPSAELVSAYSCEKRVTRQTPPSFICLAADDDVVPPMENGMV
                     MFEALRRAQVPTELHVFQEGSHGFGIRLAKGKPASAWTELFLHWGYSNGWFRDPSAAP
                     G"
BASE COUNT          598 a         1057 c         1098 g          532 t
ORIGIN      
        1 ggctggaccg gcgtgaagat gggcgcgcaa cgcccgctga ccttcggtcc cgacgagatg
       61 acccgcctga aacagatcgt gctgggcggc gaggccaagg cgtgcgctgg cggcagctat
      121 gcgttcgtgg agaatttccg cgagcgatat cttgcggccg taacggcggg ccacagactt
      181 tcgcggccga tcaaggtggt ggcggcatgc ggcaacggca ccgccggcgc ctttgcgccc
      241 gaagcgcttc ggcgcatcgg cgccgaggtg atcgagatgg acgccacgct cgactacacc
      301 ttcccgaaat acaatcccaa tcccgaagac atgcagatgc tgcacgccat ggccgatgcg
      361 gtgcgcgcgc acaaggcgga gctgtgcatg ggcttcgacg gcgacggcga ccgctgcggc
      421 gtggtggacg acaagggcga ggagattttc gccgacaagg tgggcgtgct cctggcgcgc
      481 gatctgtcgt cgcgcatccc gaacgcgcag ttcgtggtcg atgtgaaatc gaccggcatc
      541 tatctgatgg acccggtgct gaagcagaac ggcgtgcgcg tcgattactg gaagaccggc
      601 cattcctaca tcaagcggcg cacggcggaa ctgaaggccg tggccgggct ggagaagagc
      661 gggcattatt tcttcaaccc gccgctcggg cgcggttacg acgacggcat tctcgcggcc
      721 atcgccgtgc ttgaaatgct ggaccatgcg ggggggaaac tgtcggcgct ggtcgccggc
      781 ctgccgaaaa cctggtcgtc gccgaccatg agcccgttct gctccgacga aagcaaatac
      841 aacgtcgtcg cggcgatcac gcaggaatat gagggcatca aagcgcgcgg cgagaaggtg
      901 ctgggccaga agatcgtttc catcgtcacc gtcaacggcg tgcgcgtgac cctggaagac
      961 ggaacctggt gcctggtgcg cgcatcgtcg aacaagccct cgctggtggt cgtggtcgaa
     1021 agcccgtcgt ccgaagccaa catgcacgcg atcttcaacg acctcgacgg gcgcctgtcg
     1081 aagcatcccg acgtgggcgc gtacgatcag aagatatagt cggaacgtat tcttcgttcg
     1141 tcatggccgg gttagacccg gccatccaga gtcctttcgc tcgcgggacg ctcgttagct
     1201 gggtggccgc ctcaagggcg gccatgacga aggtaaagga aggtgcaaat taagctaacg
     1261 catcccacag catcttcgcg gccgtgatcg ccacgaacag cgcgaacagt ctgcgcagcc
     1321 ggcccctgtc ggtcatgtgg gcgatggcgg cgcctgccgg ggcggtgaaa aacgccaccg
     1381 gcgcgatcag gagaaatccg agcaggttga cataacccag cgaataaggc ggcaggccgg
     1441 ccgcgccgta gccggcgatc gccgcgccga tcgcgcccgg aatggagatg atcgccccga
     1501 acgccgaggc ggtgccgacc gcgcgatgaa tcggcacgcc gcacagcgtc atcgccggca
     1561 cgccgacggt tccgccgccg atgcccatca tggtcgagat gccgctgatg ccgaacggca
     1621 gcagcgctcc gccgatgccg ccgggcagtt tgtccgccag ccgccaggtt tctttcatga
     1681 aagccagttg cgccgccacc ggcagcgcca ccagtccgaa gatcagcgcc agcgtctgcc
     1741 cccgcgacac gcccgccagt acgccgcccg ccagcacgcc cgcgagcatc ggccatagcc
     1801 agcgcttcag cagttcgcga tcgaccgcgc cctttttgtc gtgggcgcgc agcgacgaaa
     1861 tcgaggtcgg gatgatggtg gcgagcgagg ttcccaccgc gatatgcatc cgcaggtctt
     1921 cggaaacgtt catggcgacg agcacgtgat acatcaccgg caccaccacg atgccgccgc
     1981 cgacgcccag catgcccgcg atcaggcccg ataccacgcc ggcaattaag aggcccagcg
     2041 ccagcgtgcc gatatcgccg gttgccgcaa aggttgtgta atccatctgg tcctctttgt
     2101 cgtgcggcgc gagtgaactt gcattcggcc ccgcgcgcaa gcgatgcttg gcgcaaatcg
     2161 ctcagaggga ggcgtgtatg gacagacgta cggtgttggg ccttggcgcg gcgacgctgg
     2221 ccgggctttc ggtgcgggct ttcgcggagg agatgaacgc cgagggcgtg ctgtccaccg
     2281 atccgacgga aatcgtgccc ctgtggccgg gaacgccgcc gggcggggag ggcgtcaaga
     2341 tcaaggccaa gatcgtcgaa cgcagccccg acaccaacgc ctatcacgac cgcttcgtca
     2401 gcgacatcgg catgccttgg ctttccgtgt ttcgccccga acggcccgac gggtccgccg
     2461 tgctgctcgc gccgggcggc ggctatacgc gcatcgtcat cgacaaggaa ggcgtggaag
     2521 ccgggcgccg gttcaatgct tcgggcgtca ccgtattcgt tctgtactac cgcctgcctg
     2581 ccgaaggctg ggcaaacggc aaggacgtgc cgttgcagga tgcgcagcgc gccatgcgac
     2641 tcatccgcgc cggcgcgcaa ggctacggca tcgatcccga ccgggttggc gttatgggat
     2701 tttcggcggg cggccacgtc gcggcgtcgc tggcgacgcg tcagaatgag cccgtttatg
     2761 cgccggtgga cgcggcggac cggaaagacg cccgtcccgc attcgccgcg ctgatgtacc
     2821 cggtgatcac catggaaccg ggcaggcatc agggatcgcg cgatcgtctg ctcggcgccg
     2881 aaccgtccgc cgaattggtt tctgcctatt cctgcgagaa gcgggtgacg cgccagacgc
     2941 cgcccagctt catctgcctg gccgccgacg acgatgtggt accgcccatg gaaaacggca
     3001 tggtcatgtt cgaggcgctg cgccgcgccc aggttcccac cgagctgcat gtcttccagg
     3061 aaggcagcca cggcttcggc atccgcctgg cgaaaggcaa accggcgtcc gcctggacgg
     3121 agctgttcct gcactggggc tatagcaacg gctggttccg cgatccgagc gccgcgccgg
     3181 ggtgacggtg ggcgaatagc gaatagagtt tctgcccccg cgaagcgagg cgaaggggct
     3241 ctattcgcta atcgctattt gctccttcag aaacgccagc gcgtc
//
LOCUS       DASWPU010000228         5796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_8028, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000228 DASWPU010000000
VERSION     DASWPU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8028"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1808
                     /locus_tag="VGC27_04940"
     CDS             <1..1808
                     /locus_tag="VGC27_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281950.1"
                     /translation="KYVITGLTPETHAAYCRDALAALLKALPAVSSVALRIHGESGVA
                     EGSYDFWKTVFDGVPRSGRSIEIDLHAKGIDDRMIENALATGMPVNLAPKFAAEHMGL
                     PYHQAEIRPTEIPDPGLVGKGLMTISEGQRSFTRYGYADLMRDDRKYTVRTRVFSGTQ
                     RILASGDAAAGAAYGRAFQFCGMTGAELMEPLTCRGRRGSSVEDIARSGYANAKLEPK
                     YDWQKYAAWYRTFGRALFNPDGDPEVFHRAFGKDAALESALASASRILPLVTNAYLPS
                     AACDQYWPEIYWNQPMSGEPTPNFYRDMPAPRVFQNAGALDPQLFSSCNEFAGELLGE
                     RSGKYAPLEVAAWLDGFADAATNSLKAAGQATSIDAMRLAIDIEVQALLGRFFAAKMR
                     SGVLFAIHERSNDARALHQSLLQYYEARNQWASIIKRTRGVYASDLAVSDRFSERGQW
                     SDWLAFIDWDIFEVERRRAGAKDSTDPRVIAAVQTIIRAPEAIKREPLAGTHTPPAWF
                     TPKQEVALEIAVSRPLTGARLYYRHVNQAERWTKAEMAAANGVYRASVPAAYTDSPYP
                     LQYYFELKAAPDKAWIYPGFDAELLNQPYVVLRRI"
     gene            complement(1963..3786)
                     /gene="rpsA"
                     /locus_tag="VGC27_04945"
     CDS             complement(1963..3786)
                     /gene="rpsA"
                     /locus_tag="VGC27_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY0281951.1"
                     /translation="MARHQATTKVHDQSLSSPSRDDFAVMLEESFVTQSPQEGNVIKG
                     TIVAIENDFAIVDVGLKTEGRVSLKEFSMPGVPVTVKVGDEVEVYLERVENALGEAVL
                     SRDKARREESWVRLERAFNEQTRVTGVIFGRVKGGFTVDLDGAVAFLPGSQVDVRPMR
                     DVGPLMNQPQPFQILKMDRRRGNIVVSRRAVLEERRAEERTSLVASLQEKQVVEGVVK
                     NITDYGAFVDLGGVDGLLHVTDIAWRRVSHPTEVLSVGQTVNVQIIKINHETQRISLG
                     MKQLQTDPWEGVGVKYPSGARFKGKVTNVTDYGAFVELEPGVEGLVHVSEMSWVKKNV
                     APAKIVTPGSEVEVQVLEVDSNKRRISLGLKQTQDNPWQVFAAAHPAGSVVEGEIKSI
                     TEFGLFVGVAEDIDGMVHLSDLSWDKQGDAAVADYQKGQTVKAKVLDVDVEKERISLG
                     IKQLDKDPMEKAGPLKKGSIVTVTVTEVNDGGIEVQLDGGAKSFIRRSDLARDRADQR
                     PERFGKGDKVDALVTSVDKAARKIALSIKAREITEEKEAVKQYGSSDSGASLGDILGA
                     ALRRRAKRAPDDTDEDDDKEDEKEEDAEKEVATTEKEKSGE"
     gene            complement(3892..4584)
                     /gene="cmk"
                     /locus_tag="VGC27_04950"
     CDS             complement(3892..4584)
                     /gene="cmk"
                     /locus_tag="VGC27_04950"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY0281952.1"
                     /translation="MTIIIAVDGTAASGKGTLAKKLAAHFGFAHLDSGSLYRLVALGV
                     LEAKGDPNKEEDAVAAARAVDPSRASDPAIRTVEIGNVASIVSAIPAVRAALLEFQRA
                     FAATPPGSLPGAVIDGRDIGTVVCPDAAAKLFVDARPEIRAHRRWLELAAAGKAPAES
                     AILAEILARDARDRSRPVAPLKPAPDARLLDTSDLAIDAAFAAALALVRPGVEDALKA
                     RPKGLGRPGGAS"
     gene            complement(4581..4859)
                     /locus_tag="VGC27_04955"
     CDS             complement(4581..4859)
                     /locus_tag="VGC27_04955"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0281953.1"
                     /translation="MSVNFTRRALRDLANIRAYIANDNPGAASRMAVEIIAACDRLEY
                     FSERGRPGLRRGTRELTTVWPYVIVYRVTGTNIDVLSIWHGARKRYRS"
     gene            complement(4850..5032)
                     /locus_tag="VGC27_04960"
     CDS             complement(4850..5032)
                     /locus_tag="VGC27_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281954.1"
                     /translation="MAPKPAPAADQDTQDRAEYLAAVDEGLADAKAKRTVPYEKVRRW
                     LLSWGGDKELPPPRCP"
     gene            5185..5634
                     /locus_tag="VGC27_04965"
     CDS             5185..5634
                     /locus_tag="VGC27_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281955.1"
                     /translation="MQQLQKIDIDWDIHKMIEAERRSFDEAPFLALRRLLKLPPPKLP
                     PLSEKADIGAGIPWIEDGVSVPHGALAKMEYQRGTQVYEGQFLNGKLVVNGRSFDALS
                     AAANAFAVTRDGSQTNLNGWNYWKAKFPGETTWRSLGDMRQKAKAAR"
BASE COUNT         1053 a         1839 c         1831 g         1073 t
ORIGIN      
        1 ccaaatatgt gatcacgggc ctgacgcccg aaacccacgc cgcctattgc cgcgatgcgc
       61 tggcggcgct gttgaaggcc ctgccggcgg tctcgtcggt cgccttgcgc atccatggcg
      121 agagcggcgt cgccgagggc agttacgatt tctggaagac ggtgttcgac ggcgtgccgc
      181 gcagcggacg cagcatcgag atcgacctgc acgccaaggg catcgacgac cgcatgatcg
      241 agaacgcgct ggcgaccgga atgccggtca acctggcgcc gaaattcgcc gccgagcata
      301 tgggcctgcc ctatcaccag gcggaaatcc gcccgacgga aatcccggac ccggggctgg
      361 tgggcaaagg cctgatgacg atctcggaag gccagcgcag cttcacgcgc tacggctatg
      421 ccgacctgat gcgggacgac cgcaaataca ccgtgcgcac gcgggtgttc tcgggcacgc
      481 agcgcatcct cgcctccggc gacgcggcgg cgggcgccgc ctacgggcgc gccttccagt
      541 tctgcggcat gaccggcgcg gaactgatgg agccgctcac ctgccgcggg cggcgcggca
      601 gttccgtcga agacattgcc cgcagcggct acgccaacgc caagctggag ccgaaatacg
      661 actggcagaa atacgccgcc tggtatcgca ccttcgggcg ggcgctgttc aatccggacg
      721 gcgaccccga ggtgttccac cgcgccttcg gcaaggacgc ggcgctggaa agcgccctgg
      781 cgagcgcgag ccgcatcctg ccgctcgtca ccaatgccta tctgccttcg gcggcgtgcg
      841 accagtattg gccggaaatc tattggaacc agccgatgtc gggcgaaccg acgccgaact
      901 tctaccgcga catgccggcg ccgcgcgtgt tccagaacgc gggcgcgctc gatccgcaac
      961 tgttctcctc ctgcaacgag ttcgcgggcg aactcttggg cgagcgcagc ggcaaatacg
     1021 cgccgctgga agtggccgcg tggctggacg ggttcgcgga cgccgcgacg aacagcctca
     1081 aggccgccgg acaggcgacc tcgatcgacg ccatgcgcct ggccatcgac atcgaagtcc
     1141 aggccctgct gggacggttc ttcgcggcga agatgaggtc cggcgtcctg ttcgccatcc
     1201 acgaacgcag caacgacgcg cgcgcgctgc accaaagcct gctgcaatat tacgaggccc
     1261 ggaaccagtg ggcttctatc atcaagcgga ccaggggcgt ctatgcctcc gacttggcgg
     1321 tgagcgaccg cttctccgag cgcgggcaat ggagcgactg gctggcgttc atcgactggg
     1381 acattttcga agtcgaacgg cggcgcgcgg gcgccaagga cagtacggac ccgcgcgtta
     1441 tcgcggcggt gcagacgatc atccgggcgc ccgaggcgat aaagcgcgaa ccgctcgccg
     1501 gcacgcatac gccgcccgct tggttcacgc cgaaacagga ggtggcgctg gaaatcgccg
     1561 tgtcccgtcc gctgaccggg gcgcggctct attaccggca cgtcaaccag gcggaacgct
     1621 ggacgaaggc ggagatggcg gccgcgaacg gcgtctatcg cgccagcgtt ccggcggcgt
     1681 acacggattc gccctacccg ctgcaatatt acttcgagct gaaagccgcg ccggacaagg
     1741 cgtggattta ccccggtttc gacgcggagc tgctgaacca gccctacgtg gtgctgcgga
     1801 ggatataaaa aaggcgcggg ggtgagccgc gccttttgtc attgattgag gcatccgccc
     1861 ccttcgtctc gcgcggctgg ggccgcgcga tccacttccc ccgctcccca cctacgctaa
     1921 agcttcggtg ggcagggcgg gggcagaaag gcggagcgag gcttactccc cgcttttctc
     1981 tttttcggtc gtcgcgactt ctttctctgc gtcttcttct ttttcgtctt ccttgtcgtc
     2041 gtcctcgtcg gtgtcgtccg gagcgcgctt ggcgcgccgg cgcaaagccg cgccgaggat
     2101 gtcgccgagc gaggcgcccg aatccgacga gccgtactgc ttcacggctt ccttttcctc
     2161 ggtgatttcg cgcgccttga tcgagagcgc gatcttgcgg gcggccttgt cgacgctggt
     2221 gaccagcgcg tccaccttgt cgcccttgcc gaagcgttcc ggacgctgat cggcgcggtc
     2281 gcgcgccaga tcggagcggc ggatgaacga cttggcgccg ccgtcgagct gcacttcgat
     2341 gccgccgtcg ttcacttccg tgaccgtgac ggtgacgatc gaaccctttt tcagcgggcc
     2401 ggccttctcc atcgggtcct tgtcgagctg tttgatgccg agcgagatgc gctccttctc
     2461 gacatcgacg tcgagcacct tggccttgac cgtctggccc ttctggtaat cggccacggc
     2521 cgcgtcgccc tgcttgtccc acgacagatc ggagagatgg accatgccgt cgatgtcttc
     2581 cgccacgccg acgaacaggc cgaactcggt gatcgacttg atctcgcctt ccacgacgct
     2641 gcccgccgga tgcgccgccg cgaacacctg ccagggattg tcctgggtct gcttgaggcc
     2701 gagcgaaatg cgccgcttgt tggaatcgac ttccagcacc tggacttcga cttccgagcc
     2761 cggcgtgacg atcttggccg gggcgacgtt cttcttcacc cacgacatct cgctgacatg
     2821 gaccaagcct tcgacgcccg gctccagttc cacgaaggcg ccgtagtcgg tgacgttggt
     2881 gaccttgccc ttgaagcgcg cgccggaggg atatttcacc ccgacgcctt cccagggatc
     2941 ggtctgcaac tgcttcatgc ccaggctgat gcgctgggtc tcgtggttga tcttgatgat
     3001 ctgcacgttg acggtctggc cgacgctgag aacttccgtc gggtggctga cgcgccgcca
     3061 ggcgatgtcg gtgacgtgca acaggccgtc cacgccgccc agatcgacga aggcgccgta
     3121 atcggtgatg ttcttgacga cgccttccac cacctgcttt tcctgcaagg aggcgaccag
     3181 cgaggtccgc tcctcggcgc ggcgctcttc cagcacggcg cggcgcgaca ccacgatgtt
     3241 gccgcggcgg cggtccattt tcaggatctg gaaaggctgc ggctggttca tcagcgggcc
     3301 gacgtcgcgc atcggacgga catcgacctg cgaaccggga agaaacgcca ccgcgccgtc
     3361 gagatcgacg gtgaagccgc ccttgacccg gccgaagatc acgccggtca cgcgggtctg
     3421 ttcgttgaac gcccgttcca gacggaccca gctttcctcg cgccgcgcct tgtcgcgcga
     3481 cagcaccgcc tcgcccagcg cgttctcgac gcgctcgaga tagacttcca cctcgtcgcc
     3541 gactttcacc gtcaccggca cgcccggcat ggagaattcc ttgaggctga cgcgcccctc
     3601 ggtcttcaag ccgacatcga cgatggcgaa atcgttctcg atggcgacga tcgtgccctt
     3661 gatgacgttg ccttcctgcg gcgactgggt gacgaagctc tcttcgagca tgaccgcgaa
     3721 atcgtcgcgg ctgggactgg aaagggattg gtcgtgaact ttggtggtgg cttggtgccg
     3781 ggccatgaat tcctctgggt tcgaatgcgc gtgccggccg gatcgctccg gcggacttcc
     3841 atgcggacgc atgcccgcaa ggcggtgatg gtttcggata agttcgtgcc gctaagaggc
     3901 gccgccgggt ctccctaacc ccttgggccg cgccttcagc gcatcttcga caccgggtct
     3961 taccagcgcg agggctgccg cgaacgcggc gtctatagcc aaatcggagg tatcgagcaa
     4021 gcgcgcgtcc ggcgcgggct tgagaggggc gacgggccgc gaacggtcgc gggcgtcgcg
     4081 ggccaggatt tcggcgagaa tggcggattc cgcgggcgct ttgccggcgg cggcaagctc
     4141 cagccagcgg cgatgagcgc ggatttcggg cctcgcgtcc acgaacagct tggccgccgc
     4201 atcggggcag accacggtgc cgatgtcgcg cccgtcgatc accgccccgg gcaaagagcc
     4261 gggaggcgtt gccgcaaacg cacgctggaa ctccaacagc gccgcgcgca cggcgggaat
     4321 ggccgagacg atggaggcga cattgccgat ctcgacggtg cggatggcgg gatcggacgc
     4381 gcgggacgga tcgaccgcgc gggcggcggc gaccgcatcc tcttccttgt tgggatcgcc
     4441 ctttgcttcg agcacgccca gcgccaccag ccggtagagc gatccggaat ccagatgggc
     4501 aaaaccgaaa tgcgcggcca gcttcttcgc cagcgttccc ttgcccgagg cggcggttcc
     4561 atcaacggcg atgatgatgg tcatgaacgg taccgcttcc gtgcgccatg ccagatggag
     4621 agaacgtcga tattcgtgcc cgttacgcga tagacaatca cataaggcca aacggtcgtc
     4681 agctcacggg taccgcgccg caaccctgga cgtccacgtt cggaaaaata ctcaagcctg
     4741 tcgcaagccg cgataatttc aacggccatg cgagaggcgg caccgggatt atcgtttgcg
     4801 atataagccc ggatattggc aaggtcgcgc aatgcccggc gggtgaaatt tacggacatc
     4861 tgggcggagg caattccttg tcgccgcccc atgacagaag ccagcgccgg accttctcgt
     4921 atggaacggt gcgctttgcc ttggcatcgg caagcccctc gtccacggcg gcgagatatt
     4981 cggcgcggtc ctgggtgtct tgatcggcgg cgggtgcggg tttcggtgcc atggcgttat
     5041 cataatccac gttgaacgaa gagcaaaagc catgtttcaa ggccatattt ctcctctact
     5101 cataattact ccttgacaga gtatttctaa gggggtaata ggaaacatgc gggtatcatt
     5161 gcggcctttg aaaggaaaat ttcaatgcag cagctccaaa aaatcgatat cgattgggac
     5221 attcataaga tgatcgaggc tgagcgtcga agttttgacg aagcgccctt tttagcgttg
     5281 cgcaggcttc tcaagcttcc accgccaaag cttccgccgc tttctgaaaa agccgacatc
     5341 ggcgcgggta ttccttggat agaagacggc gttagtgttc ctcatggcgc gttggctaaa
     5401 atggaatacc agaggggaac acaggtttat gagggtcaat tcctcaatgg aaaactggtg
     5461 gtgaatggta ggtcgttcga cgcactctct gctgccgcaa atgccttcgc agttacaaga
     5521 gacggatcac agacaaatct taatgggtgg aattattgga aagcaaagtt cccgggtgaa
     5581 acgacgtggc ggtctctagg tgatatgcgg caaaaagcaa aagcggcgcg ctaaccaacc
     5641 tcattcgact cataacccga aatgtgtgtg atttaatcat acactgtgct atcctcctcc
     5701 gacaaaaagg agattcagcc atgcggagca catcccccct cagcatcacc ctgccccacg
     5761 acatggcggc tatggtcaaa gcgaaggtcg cttccg
//
LOCUS       DASWPU010000229         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_87249,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000229 DASWPU010000000
VERSION     DASWPU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87249"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VGC27_04970"
     CDS             complement(<1..393)
                     /locus_tag="VGC27_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HEY0281956.1"
                     /translation="MTQAIWTQTYDVNTIVLNSQKRLGLSGLLNLLQDAAWLHAKHVG
                     WGYEDLIRSGTIWVLARQKLVMTDWPDWGDVVTIRTWGRPVPGALALREYEIIVGDRK
                     LGECTTSWLTLDWQTRRPKKLDRDSFDQK"
     gene            complement(505..753)
                     /locus_tag="VGC27_04975"
     CDS             complement(505..753)
                     /locus_tag="VGC27_04975"
                     /inference="COORDINATES: protein motif:HMM:NF018343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HEY0281957.1"
                     /translation="MIEKNEEYTEETLEPGRIAYIKPTGSAEARRLGLIPPGIELPPG
                     VKLYVLHASDGSILGFTDAYDSAYGAAVQNELTPVSLH"
     gene            complement(750..1187)
                     /locus_tag="VGC27_04980"
     CDS             complement(750..1187)
                     /locus_tag="VGC27_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY0281958.1"
                     /translation="MNRTVYFNSPFLLGFEHLERLLERTAKSAGDGYPPYNIEQTGED
                     SLRIALAVAGFSREELNVTVEDRQLVIRGKQSEGGDRNFLHRGIAARQFQRAFVLAEG
                     IEVTGAALENGLLTIELSRPIADTIVRTIDIKAGHLEPKRSVS"
     gene            1349..3199
                     /locus_tag="VGC27_04985"
     CDS             1349..3199
                     /locus_tag="VGC27_04985"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF005698.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY0281959.1"
                     /translation="MTRPCLILDDAAQGLVQAFMRPTATIRADTTEEVPAALAAIEAA
                     CAAGRHVAGYFSYELGYVLEARLLPLLPAARTVPLLWFGVFSAPETLGEEAFDAWCEG
                     RAYAGPLVHEWDAVAYRKRFDRVRDLIAAGDLYQANLSFRSRFAFLGDPLTLYSDLRA
                     RSAAPYGAYLDDGERQILSLSPELFFDLAAGGKITARPMKGTCARGDNPVADAEARAR
                     LAASPKDRAENLMIVDLLRNDLGRVAGIGSVTAGDLFAVETYPTVHQMVSTVTARLKP
                     GTGVKEIVRALFPCGSVTGAPKIRAMEVIAELEQSPRGVYCGAIGHFAPDGSARFNVA
                     IRTLTIAGGRGTLGIGGAVVQDSVADGEYAECLLKALYFEAPRRPLELIETLRFSPGE
                     GFIRSDLHLERMARSAETFGMKFDIRQAHRALDEVLPPPLRVEAVYESSCGAFVGRAR
                     KNPPADFSVGGNDNVLRARLTLNESGEFACTAAPLPPNPQSWTYVVSPRRTAADALAR
                     HKTNRRDLYESEFARLAEASGCDEVVFLNPRGEVAEGSRTNVFVAREGMLLTPPLSAG
                     ALDGCLRRALIGEGRCMEALLLPGDLDGEVYLGNSLRGLIRAVPAAPTGV"
BASE COUNT          527 a         1013 c         1059 g          629 t
ORIGIN      
        1 cttctgatcg aacgagtcgc gatcgagctt cttcgggcgc cgcgtctgcc agtcgagggt
       61 gagccagctg gtggtgcatt cgcccagctt gcggtctccc acgatgatct cgtattcgcg
      121 cagcgcgagc gctcctggca ccggccgccc ccaggtgcgg atggtcacga cgtcgcccca
      181 gtcgggccag tccgtcatga ccagcttctg gcgcgcgagc acccagatcg tgccgctgcg
      241 gatcaggtcc tcgtagcccc aaccgacatg cttggcatgg agccaggctg cgtcctgcaa
      301 gagattcagc aagcctgaga ggccaagccg cttctgggag ttcaacacga tggtgttgac
      361 gtcgtaggtc tgggtccaga ttgcctgtgt catttcagag caaagctgta ttgcagcggt
      421 ggtctgaagg gaaggggtga atagcgagca gcgtaaggaa acaacctcct attcgctatt
      481 ccctattcgc aactcgcgct ttgttcagtg caagctcacc ggcgtgagtt cgttctgcac
      541 cgccgcgcca taggcggagt cgtaggcgtc ggtgaaaccg agaatgctgc cgtcgctggc
      601 atgcagcacg tagagcttca cgccgggcgg caattcgatg ccgggcggga tgaggccgag
      661 acggcgggct tccgcgctgc cggtcggctt gatgtaggcg atgcggccgg gttcgagcgt
      721 ttcttccgtg tattcttcgt tcttctcgat catgacacgg acctcttcgg ttccaggtgc
      781 cccgccttga tgtctatggt acgcacgatt gtgtcggcta tagggcgcga caattcgatg
      841 gtcagcaggc cgttttccag tgccgcaccg gtcacctcga tgccttcggc caggacgaag
      901 gcgcgctgaa actgcctggc cgcgatgccg cggtgcagga agttgcggtc gccgccttcg
      961 gattgcttgc cgcggatgac aagctggcgg tcttccaccg tcacgttcaa ctcttcgcgc
     1021 gagaaaccgg ccaccgccag agcgatgcgc agactgtctt cgccggtttg ttcgatgttg
     1081 tagggcggat acccgtctcc ggcgcttttg gccgtgcgct ccagcaggcg ttccagatgc
     1141 tcgaagccca acaggaacgg gctgttgaaa tagacggtcc tgttcatcgt caaagccctc
     1201 cttaagcggc tttgttgtca tcgagcggcc caataaggcg ccgcctacac ttacaatgtg
     1261 gggtttcgca gccggcaatc aagccatggc tggagttgac ggtgcggata ttgcccctaa
     1321 gcgacgggca gggcacaagg aatcggccat gaccagacct tgcctgatcc ttgacgacgc
     1381 cgcccagggg ctggtgcagg cctttatgcg gccgacggcg acgatccggg cggatacgac
     1441 ggaagaagtc cccgccgcgc tggccgcaat cgaggccgcg tgcgccgccg ggcggcatgt
     1501 cgcaggatat ttttcctatg agcttggcta tgtgctggag gcacggttgc taccgttgct
     1561 ccctgccgcg cgcaccgtgc ccctgctgtg gttcggcgtc ttcagtgcgc ccgagacctt
     1621 gggcgaggag gcgttcgatg cttggtgcga aggccgcgcc tatgccggtc ctttggtcca
     1681 cgaatgggac gccgtggcgt accgcaagcg cttcgaccgg gtgcgcgatc tcatcgcggc
     1741 cggcgatctc tatcaggcga atttgagttt ccgttcgcgc ttcgccttcc tgggcgatcc
     1801 gctgacgctg tacagcgatt tgcgcgcccg ctccgccgcg ccttacggcg cctatctcga
     1861 cgacggcgaa cggcaaattc taagtctttc gcccgaactc ttcttcgatc ttgccgccgg
     1921 cggaaagatc acggcgcgtc cgatgaaagg cacttgcgcg cgaggggaca atcccgttgc
     1981 cgacgccgag gcgcgggcgc ggctggccgc cagtcccaag gaccgcgccg aaaatctgat
     2041 gatcgtcgat ctcttgcgca acgatctggg ccgggtcgcc gggatcggca gcgtcacggc
     2101 cggcgatctg ttcgcggtgg aaacctatcc gacggtgcat cagatggttt ccaccgtgac
     2161 ggcgcggctc aagcccggca cgggcgtgaa ggaaatcgtg cgcgcgctgt ttccctgcgg
     2221 ctcggtcacc ggcgcgccga agatccgggc catggaggtg atcgcggagt tggagcaaag
     2281 cccgcgcgga gtctattgcg gggcgatcgg ccatttcgcg cccgacggtt ccgcccgctt
     2341 caatgtcgcg atccgcacgc tcacgattgc cggcgggcgc ggcacgctcg gcatcggcgg
     2401 cgccgtggtg caggattccg tcgccgacgg cgagtatgcc gaatgcctgc tgaaggcgct
     2461 ttatttcgag gcgccgcgcc ggccgctgga gctgatcgaa acgctgcgct tctcgcccgg
     2521 cgaagggttt atccggagtg atctgcacct cgaacgcatg gcgcggtccg cggagacgtt
     2581 cgggatgaag ttcgatatcc ggcaggctca tcgcgctctt gacgaagtgc tgcctccccc
     2641 cttgagggtc gaggccgtat acgaaagctc ctgcggagcc ttcgtaggcc gagcgcggaa
     2701 aaatccgcct gcggattttt cggtgggggg taatgacaat gtcttgcggg cacgcctcac
     2761 cttgaacgaa tccggcgaat tcgcttgcac ggcggctccg ctgccgccga atccgcagag
     2821 ctggacatac gtcgtctcgc cgcggagaac ggccgccgac gcgctcgccc gccacaaaac
     2881 gaaccggcgc gatctctacg aaagcgaatt cgcccgcctg gcggaagcgt cgggctgcga
     2941 cgaggtggtg ttcctcaacc cgcgcggcga agtggccgag ggcagccgca ccaacgtctt
     3001 cgtcgcgcgc gagggaatgc tgctcacgcc gccgctctcg gccggggcgc tcgacggctg
     3061 tctgcgccgc gccctgatcg gcgaaggacg ctgcatggaa gccctgcttc tgcccggcga
     3121 tcttgacggc gaagtctatc tcggcaattc cctgcgcggg ttgatccggg cggtgccagc
     3181 agcgcccaca ggtgtctgag acaaatcaat gttgtcatcc ccggcgag
//
LOCUS       DASWPU010000230         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_50876,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000230 DASWPU010000000
VERSION     DASWPU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50876"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VGC27_04990"
     CDS             complement(<1..650)
                     /locus_tag="VGC27_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY0281960.1"
                     /translation="MNMKSALTAAVLIIGDEILSGRTQDTNTNTIARFLGALGIDVKE
                     VRVVGDVEDEIVAALNALRARYTYVFTTGGIGPTHDDITADAVAKAFGVGINYHPEAL
                     AILAARIKPGHFNEMRKRMARIPFGASLIKNNVSAAPGFQIENVFVMAGVPSIMQAML
                     EDVGPRLARGRPIKAVTIAAPIAEGTVAAALAKLQDEHATAIRVLESRTKKADGFA"
     gene            complement(703..939)
                     /locus_tag="VGC27_04995"
     CDS             complement(703..939)
                     /locus_tag="VGC27_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281961.1"
                     /translation="MRWIGMLLAAGLTLASSSAGTDPAWNGPGWYVVAYQDAVVLWSG
                     PYASEEDCELAKPPDNVPPDFGFSCTFFDQAPEQ"
     gene            1128..1964
                     /gene="map"
                     /locus_tag="VGC27_05000"
     CDS             1128..1964
                     /gene="map"
                     /locus_tag="VGC27_05000"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY0281962.1"
                     /translation="MFSLMPETETFEAARRAHFAVALHTEKDFEGMRRAGRLAAEVLD
                     LLVPEVKPGVTTAHLDKLAYDYIISHGALAACLGYRGYRHTLCNSINHVVCHGIPSDK
                     PLRDGDILNIDVTLILDGWHGDTSRMYLAGEVKRKAARLVDITYESMLRGIAAVKPGA
                     TTGDIGHAIQSYAEGEEHCSVVRDFCGHGVGRVFHALPNIVHYGKPGQGIGLKPGMFF
                     TVEPMINLGGYGVKVLSDGWTAVTRDRSLSAQFEHTVGVTEDGVEVFTYSPKGWHKPP
                     YF"
BASE COUNT          394 a          712 c          671 g          397 t
ORIGIN      
        1 ctggcgaagc cgtccgcttt tttcgtccgg gattccaaca ccctgatggc cgtggcgtgc
       61 tcgtcctgga gcttcgccag agcggccgcg acggtgcctt cggcaatggg cgcggcgatc
      121 gtcacggcct tgatcgggcg cccgcgggcg agcctgggcc caacatcctc gagcatcgcc
      181 tgcatgatgc tcggcacccc cgccatcacg aagacgtttt cgatctggaa tcccggcgcg
      241 gccgagacgt tgttcttgat caggctggcg ccgaacggaa tccgcgccat gcgcttgcgc
      301 atttcgttga aatgtcccgg cttgatgcgc gccgccagga tcgccagcgc ctccgggtga
      361 taattgatgc ccacgccgaa cgccttggcg acggcgtcgg cggtgatatc gtcatgggtg
      421 ggaccgatgc cgccggtggt gaaaacatag gtgtagcgcg ctcgaagcgc attcagcgcg
      481 gccacgatct cgtcttccac gtcgccgaca acgcggactt ccttgacgtc gatgcccagc
      541 gcgccaagga accgggcgat ggtgttggtg ttcgtgtcct gggtgcggcc ggacaggatt
      601 tcgtcgccga tgatcagcac ggcggccgtg agcgcggatt tcatattcat cgcgcgcact
      661 gtggccttgc cgccgctcag atttcaatat gaccgattga aactattgtt ccggggcttg
      721 atcgaagaag gtgcaggaaa agccgaaatc cggcggaaca ttgtccggcg gtttggccag
      781 ttcgcagtcc tcctcgctgg cgtaaggccc ggaccacagg acgacggcat cctgataagc
      841 caccacgtac caacccggtc cgttccaagc cgggtccgtg ccggcggaac tcgatgccag
      901 agtgaggcca gccgccagca gcataccgat ccagcgcatg tacacctcct acgtgcatga
      961 agacgccgca ttctagcgca ttcgccggcc tgcccctacg tcagggccac accggcgtat
     1021 cgatcatggg caaagcggcg ctggcgggca ctactctgta aggtataaga gacaagctgg
     1081 caaagcccgg cgccgagcct tacattccgc ttcagacaga gtccgccatg ttttccttga
     1141 tgcccgaaac cgaaaccttc gaagccgcgc gccgcgccca tttcgcggtg gccttgcaca
     1201 cggaaaagga ctttgaaggc atgcgccggg ccggacggct ggccgccgaa gtgctcgatc
     1261 tgctggtgcc ggaagtgaag ccgggcgtaa ccaccgccca tctcgacaag ctggcttacg
     1321 attacatcat cagccacggc gcgctggcgg cctgcctggg ctatcgcggc taccgccaca
     1381 cgctctgcaa ctcgatcaac catgtggtct gccacggcat cccctcggac aagccgctgc
     1441 gcgacggcga catcctcaat atcgacgtga ccttgatcct cgacggctgg cacggcgaca
     1501 ccagccggat gtatctggcc ggcgaggtca agcgcaaagc cgcgcggctg gtcgacatta
     1561 cctacgaatc catgctgcgc gggatcgcgg cggtgaagcc gggcgccacc accggcgaca
     1621 tcggccacgc catccaatcc tatgccgaag gcgaagagca ttgttcggtg gtgcgcgatt
     1681 tctgcgggca cggggtcggc agagtcttcc atgccctgcc caacatcgtg cattacggca
     1741 agccgggcca aggcatcggc ctcaagcccg gcatgttctt caccgtcgag ccgatgatca
     1801 atctcggcgg ctatggggtg aaagtcctga gcgacggctg gaccgcggtg acgcgcgatc
     1861 gttcgctctc cgcgcagttc gaacataccg tgggtgtcac agaagatggc gtcgaagtct
     1921 tcacctattc gcccaaaggc tggcacaagc cgccatattt ctgacgtcga gcggccgttc
     1981 gaggatgcgc ctcattattt cggccaccgc gaccggctgc gccagcgctt cctcgcgggc
     2041 ggccccgagt ccatgcccga ttacgaactg atggaactgg tgctgttcgc cgccatcccg
     2101 cggcgcgacg tcaaaccgct ggccaaggcg ctgattgccc gtttcggcgg cttcgcggaa
     2161 gccatcgcag cgcc
//
LOCUS       DASWPU010000231         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_38918,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000231 DASWPU010000000
VERSION     DASWPU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38918"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1055)
                     /locus_tag="VGC27_05005"
     CDS             complement(join(113..719,718..1055))
                     /locus_tag="VGC27_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970797.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY0281963.1"
                     /translation="MTRPLSMDLRERAMVRLAAGESIRAVAAALSISPSSVVKWSQRL
                     RTTGSAAPGRIGGYVPPKIAGAHRDWLIERTRASAFTLRGLVAELGERGLSVDYRTVW
                     KFVHREGLSFKKKTVLASEQDRPDVARRRARWKAYQNRIDPSRLVFIDETWAKTNMAP
                     LRGWAPKGQRLRAKVPFGHWKTMTFLAALRHDRIAAPWVFDGPINAESFLIYVEQVLV
                     PTLHPGDVVVMDNLGSHKGPAVRAAIRAAGAHLIFLPPYSPDLNPIEQVFAKLKHLLR
                     KAAERSVDATWKRIGALLGKFSPIECANYFLNSGYASA"
     gene            complement(1146..>1526)
                     /locus_tag="VGC27_05010"
     CDS             complement(1146..>1526)
                     /locus_tag="VGC27_05010"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281964.1"
                     /translation="DLKYAAGGIVDIDFIAQYLQLVHAADKPEILDVNTLHVLDNAAR
                     LGILPQSAAEILRPAARLYHDLTQILRLCVSDKFRPETSGEDLLRIMARAGDAPDFSS
                     LEARVTETQTEVRRVFLALVQSVN"
BASE COUNT          284 a          481 c          461 g          300 t
ORIGIN      
        1 ggagcgatta tgggcgctcc gctgcaggtt ttcaaacagt caaatcgatt cgcgcgcagc
       61 gccggtcaca gccgggactc gattgatttg cctgacctag agcgggatga ctctacgctg
      121 acgcatatcc ggaattgagg aagtaattgg cgcattctat tggcgagaac ttgccgagga
      181 gtgcgccgat ccgcttccag gtggcatcga cgctgcgttc ggcggccttt cgcagcaggt
      241 gtttgagctt ggcgaagacc tgttcgatcg ggttgagatc tgggctgtag ggcggcagga
      301 agatcagatg ggcgccggcg gctctgatcg cggcgcgcac cgctgggccc ttgtggctgc
      361 cgagattgtc catcaccacg acgtcgcccg gatggagggt ggggaccagc acctgctcga
      421 catagatcag gaagctttcg gcgttgatcg ggccgtcgaa cacccagggc gccgcgatcc
      481 ggtcatgtcg cagcgcagcc agaaacgtca tggttttcca gtgtccgaac gggactttgg
      541 cccgcagccg ctggcctttt ggagcccatc cgcgcagcgg cgccatgttg gtcttggccc
      601 aggtctcgtc gatgaacacc agtcgagagg gatcaatcct gttctggtag gccttccacc
      661 gcgctcgcct gcgggcgacg tcgggccgat cctgttcgct ggccagcacg gttttttttg
      721 aagctcagcc cctcacggtg cacgaacttc cacaccgttc gataatccac cgacagcccg
      781 cgttcgccca gttccgccac cagcccgcgc aacgtgaagg ccgacgcacg ggtgcgctca
      841 atcagccaat cccgatgcgc tccggcgatt ttcggaggca cataaccacc gatgcgtccg
      901 ggcgccgcgc tgcccgtggt tcgtagccgt tgcgaccact tcacaacact cgaagggctg
      961 atgctcagcg ccgccgctac cgcgcggatg ctttcacccg ccgcaagccg caccatggcc
     1021 cgctcgcgca aatccatcga caggggtcgt gtcatccctg ctggcctcca atcccagcat
     1081 caagcttgaa tcagaaaccg cccgatttgg gaatccccat cgattcagaa aagcgtcatc
     1141 tcgctctaat tgacgctctg gaccaacgcc aaaaacaccc ggcgcacctc ggtctgcgtc
     1201 tccgtgaccc gcgcctcgag cgacgaaaag tccggtgcgt cgccggctcg cgccatgatg
     1261 cgcaaaaggt cttctcctga tgtttccggc ctgaatttgt cgctgacgca aagccgcagg
     1321 atttgcgtca ggtcatgata gaggcgtgcc gcaggccgca ggatttccgc ggccgattgc
     1381 ggcagaatgc caagccgcgc ggcgttgtcg agcacatgca gcgtgttgac gtcgagaatc
     1441 tccggcttgt cggcggcatg aacaagctgg agatattgag cgatgaaatc gatgtcgacg
     1501 atgccgcccg cggcatattt cagatc
//
LOCUS       DASWPU010000232         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_112258,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000232 DASWPU010000000
VERSION     DASWPU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112258"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VGC27_05015"
     CDS             complement(<1..694)
                     /locus_tag="VGC27_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499933.1"
                     /note="type IV secretion system VirB4 family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HEY0281965.1"
                     /translation="MLNLAEYRHKTDRLADHLPWAALIAPGVILNKDASFQRTFRFRG
                     PDLESATEAELVAACARVNNVLRRFGSGWALFFEAERFVAPDYPESHFPEPASWLVDW
                     ERRAAFEEGTLFDASGQASTRRCEHYENAYFLTLIYMAPVDHVARAERALIESDDEET
                     GRDWHRELDGFLAETNRALDLLGQLMPEIRALDDAETLTYLHRTISTKRHDVRVPETP
                     LYLDAILADESLS"
     gene            complement(688..945)
                     /locus_tag="VGC27_05020"
     CDS             complement(688..945)
                     /locus_tag="VGC27_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HEY0281966.1"
                     /translation="MSIDGFEVPLHRALTEPILLAGAPRAIAIVNGTVAAALGLGLRL
                     WIAGLLLWLIGHSIAVFAAKRDPDFVDVLRRHLRQRTHLRC"
     gene            complement(903..1208)
                     /locus_tag="VGC27_05025"
     CDS             complement(903..1208)
                     /locus_tag="VGC27_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009802150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEY0281967.1"
                     /translation="MLVSSAYAAGTNMPWEQPLQQILDSIQGPVAKIVAVIVIIMTGL
                     TLAFGESAGGFRRLIQIVFGLSIAFAASSFFLSFFSFAGGALVQLASTASKCLCIAR"
     gene            complement(1464..1670)
                     /locus_tag="VGC27_05030"
                     /pseudo
     CDS             complement(1464..1670)
                     /locus_tag="VGC27_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
BASE COUNT          357 a          504 c          522 g          292 t
ORIGIN      
        1 cgctcaggct ttcgtcggcc aaaatggcgt ccagatagag tggcgtctcc ggcacgcgca
       61 catcatggcg cttggtcgag atggtgcgat gcagataggt cagcgtctcg gcgtcatcga
      121 gggcgcgaat ttccggcata agctggccaa gaagatcgag cgcgcgattg gtctcggcga
      181 ggaagccgtc caactcgcgg tgccagtcgc ggcctgtttc ttcgtcgtcg ctttcgataa
      241 gggctcgctc ggcgcgcgcg acatgatcga ccggcgccat atatataaga gtgagaaaat
      301 aggcgttctc ataatgttcg caccgcctcg tgctcgcctg ccccgacgca tcgaataacg
      361 ttccttcctc gaaggcggca cggcgctccc agtccaccag ccaggaggct ggctcgggga
      421 agtgactctc aggatagtcc ggcgcgacga accgctcggc ctcgaagaac aaggcccagc
      481 ccgagccgaa acgtctcagc acattgttga cgcgcgcaca tgccgcaacg agttcggctt
      541 cggtggcgct ctcaaggtcc ggcccgcgaa accgaaacgt ccgctgaaag ctcgcgtcct
      601 tgttcaggat cacgccgggg gcgataagcg ccgcccaggg cagatgatcg gccagccggt
      661 ccgtcttatg gcgatattcg gcaaggttca gcatctcaga tgtgtccttt ggcggagatg
      721 ccggcgcagc acgtccacga aatcgggatc gcgcttggcg gcaaacacgg cgatggaatg
      781 gccgatcagc caaagcaaca gacctgcaat ccacaggcgc agccccaaac ccaaggcggc
      841 agcgacggtg ccgttgacga ttgcgatggc gcgcggcgcg cccgccagca ggatcggttc
      901 ggtcaacgcg cgatgcagag gcacttcgaa gccgtcgatg ctaattgcac cagtgctccg
      961 ccagcaaagg agaagaacga aaggaaaaag ctcgacgcag cgaacgcgat cgacaggccg
     1021 aagacgatct ggatcaaccg cctaaagcca ccggcgctct cgccgaaggc cagcgtcagt
     1081 ccggtcatga tgatgacaat gacggcgacg atcttggcga ctggcccctg aatggagtcg
     1141 aggatttgtt gcagcggctg ttcccacggc atgttcgtgc ccgcggcata ggcgctggaa
     1201 acgagcatcg attgaactgc aatcgcgaga gccagagggc gaacaaaaca tttgtaaccg
     1261 ggcatagggt tctcctgtcg aatcaaatgg cgtgaagttt tttggacgtt tgattgaacg
     1321 agttcgtagt cgccgtcggc cgccaaacca cgcacttctg cgatggtctc gatgcgccgt
     1381 gtcagtccgc gccctgcgat aaacaccacc agatcgatcg cttcggcgat caaccggcgc
     1441 ggcacggtga ccacgctttc ctgcaccaat tgttcgaggc gatagagagc ggacgatgcg
     1501 gaattggcat gaacggtggc gatcccgccg ggatgtcccg tgttccaagc cttgagcatg
     1561 tccagggctt cgggtccgcg cacttcgccc acgataatgc gatccggcct cagacgcagt
     1621 gtcgagcgaa cgaggtccgc gagcgaaacg ctgccagcac gggtgcgcag cgcca
//
LOCUS       DASWPU010000233         6914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_20425,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000233 DASWPU010000000
VERSION     DASWPU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20425"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1011)
                     /gene="gluQRS"
                     /locus_tag="VGC27_05035"
     CDS             complement(136..1011)
                     /gene="gluQRS"
                     /locus_tag="VGC27_05035"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY0281968.1"
                     /translation="MIVTRFAPSPTGLLHLGHAFAAITACEAGERFLVRVEDIDAGRC
                     KPEYEAALLDDLEWLGLGFERPVLRQSARLAAYRDALNILDARGLLYPCFCTRKEIVE
                     EITRSAAAPHYFDSNAAARLGPDGPLYPGTCRGLDGNERARRIAGGAAYALRLDAAKA
                     AREAGSLFFEEGGAGPNGETGRIEVQPLKFGDVVLARKETPAAYHLAVVVDDAFQGVT
                     LVTRGNDLFASTHVQRVLQALLGLPAPAYAHHRLILDEAGRKFSKRDRAVTLRALREG
                     GATPEEIRIRLRPRR"
     gene            1041..1748
                     /locus_tag="VGC27_05040"
     CDS             1041..1748
                     /locus_tag="VGC27_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281969.1"
                     /translation="MALGSLSGPRLEPLKGPATHLVVLCHGYGADGNDLIGLAPHWQS
                     LLPGAAFAAPNAPQPCPGAGYQWFPLARIDPEEMHRGVVAAAGALEAFLSQELARLKL
                     PPDRLALVGFSQGTMMALHVGLRGALKPAAIVGFSGLLTGPPSEIKDAPPVLLGHGEA
                     DNVIPPQALFVTAAALGAAGLCVQWHLSPGLPHGIDATEIALAGRFLSLAFGGKLKAS
                     GETSCVLGVANPSQRIV"
     gene            1911..2405
                     /locus_tag="VGC27_05045"
     CDS             1911..2405
                     /locus_tag="VGC27_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0281970.1"
                     /translation="MSYFPLSLWSWQDAIKAVFLERVNILEEYDRTVHSPSFEMRLPS
                     VVSLKTYIRPARNPAFTRFNVFLRDRFECQYCGADEDLTFDHVIPRSRGGRTTWDNVV
                     TACAPCNLLKGGKLPHRTGMQPRHRPHKPTVNELRNNGRLFPPNYLHQSWADYLYWDS
                     ELEP"
     gene            complement(2520..3089)
                     /locus_tag="VGC27_05050"
     CDS             complement(2520..3089)
                     /locus_tag="VGC27_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEY0281971.1"
                     /translation="MTANRRAPRPAPPGVGGKDEIAAMIRVDHAGEYGAVRIYQGQLA
                     VFGADASKAKTVAAIRHMAEQEQEHLKAFDTLINGRRVRPTALEPVWRVAGFALGAAT
                     ALMGEKAAMACTAAVEEVIDRHYAGQIERLGDSDPALKQTVEKFRADEIAHRDTALAH
                     GAEGAPAYALLSGAIKTGCRLAIALSTRI"
     gene            complement(3089..3592)
                     /locus_tag="VGC27_05055"
     CDS             complement(3089..3592)
                     /locus_tag="VGC27_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY0281972.1"
                     /translation="MSANQTVILAGVYSLAMILGTQFFQYVVGVPPCEMCYWQRWPLE
                     AAIVLCLGGAGLFAAGIAGRQTITALAWIALILIATSGIIGAWQAGVEWKIIPGPTSC
                     TGERFEFHGAADLVNYKAVVRCDVVAWKLFGISLAGYNTIFSLGAAALGAYFLAIKRT
                     FLLRRRR"
     gene            3861..4550
                     /locus_tag="VGC27_05060"
     CDS             3861..4550
                     /locus_tag="VGC27_05060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY0281973.1"
                     /translation="MKIEGRAVEVSVDLNPRARRLIVKVHPASGEVSVVAPSKTALER
                     ALEFARGQSDWIARQLAHVPSRVALVPGAHIPFRGRDHLIARCEEARVPVWIDETAVV
                     IRVGGRAEHTPRRLLDFLKRQARRALETRSIELAAKLGLRPRRITVRDTASRWGSCSS
                     TRSLSFSWRLILAPAFVLDYVVAHEVAHLREMNHGARFWRLVHELVDDVERPQAWLRQ
                     HGTSLHRYAAK"
     gene            complement(4776..6719)
                     /locus_tag="VGC27_05065"
     CDS             complement(4776..6719)
                     /locus_tag="VGC27_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY0281974.1"
                     /translation="MAPRQTENDRRTRARIAAIAEGGSEPPRPRRRRSWPYVVTLVMA
                     WSVIFGAIFFSHFLSDLPDVGSLLVNGPSRDITILDVRGRLIARRGLTQGIMVDVGKL
                     PPYVANAFIAIEDRRFREHFGLDPVGLTRAAVENMMRGHVVQGGSTLTQQLAKNLFLE
                     SGRTLDRKIQEAILALYLESRYSKDQILTLYLNRVYFGAGVYGIEAAAERFFGKRAEK
                     LSLPEAAMLAGSVKAPARYNPLADPDASQARAQIVLRVMEDSGFIDEAARRDAQATRA
                     RVVRGTATPGSGYFADWVISQIPGYVGDAKKALIVDTSFDLDAQTEAERAVTAGLREE
                     GTKLRARQAALIAMTPDGAVRAMVGGMSYEQSPYNRAADAQRQPGSAFKPFVYLAAFE
                     HGRKPSDVMNDRPVNINGWRPSDFEGKFQGEISLTKAFADSANSVAAQLTAEVGPATV
                     ARTAKRLGIATPLMAVSSLALGTSVVTPLELTAAYAPFANGGDGVLPYGIVRMRTKDG
                     EVLWQRKSSGLGRVMSSDNLAAMTALMTEVMTTGTGRAARLADRPSAGKTGTTQDYRD
                     AWFVGFSADLVCGVWVGNDDNTPMRRATGGGLPARIFKNFMEDAEFGLPARPLAGQAP
                     AAAPPPEEGDGLQQLLDRLFSGT"
BASE COUNT         1205 a         2250 c         2214 g         1245 t
ORIGIN      
        1 acatcgcaac cctcgaagag gccggttacg ccgccgtgga aaaggatttc gtcggcaagc
       61 ggccgcgcac ccgcatcgcg ctgacaaaat cgggccgccg cgccttcgag gattacatcg
      121 cctatctgcg cgacatcatc gacgcggcct gagccggatt cggatttctt ccggcgtcgc
      181 gccgccctcg cgcagcgcgc gcaaagtcac ggcgcggtcg cgcttggaaa acttcctgcc
      241 cgcctcgtcg aggatcagcc ggtgatgggc gtaagcgggc gccggcaatc ctagcagcgc
      301 ctgcaacact ctttgcacat gggttgaggc gaacagatcg ttgccgcgcg tcaccagcgt
      361 gacgccttgg aaggcatcgt ccaccacgac ggcgaggtga taggcggcgg gcgtttcctt
      421 gcgcgccaga accacgtcgc cgaacttgag cggctgcacc tcgatgcgtc cggtctcgcc
      481 gttcggacct gctccgcctt cttcgaagaa caggcttccc gcttcccgtg ctgctttggc
      541 cgcatccagc ctcagcgcat acgccgctcc gccggcgatg cggcgggcgc gttcgttccc
      601 gtccaggccg cggcaggtgc cgggataaag ggggccgtcc gggccaagcc gcgcagcggc
      661 gttggaatca aaataatgcg gcgctgcggc cgagcgcgtg atctcctcga cgatctcctt
      721 gcgcgtgcaa aagcagggat agagcaaccc gcgcgcgtcc aagatattca atgcgtcgcg
      781 ataggccgcc aggcgcgccg actggcgcag caccggccgc tcgaagccga ggcccagcca
      841 ttccagatca tcgaggagcg ccgcttcgta ttccggcttg cagcgcccgg cgtcgatatc
      901 ttccacacgc accaaaaagc gttcccccgc ctcgcaagcc gtgatagccg caaaggcatg
      961 gcccagatgc agcaatccgg ttggactcgg ggcaaagcgc gtgacaatca tggggtgcaa
     1021 tctattcgga aagaccgcag atggcactcg gctcgttatc cggtccgcgt cttgagccgc
     1081 tgaaaggccc ggccacccat ctggtcgtgc tctgccacgg ctatggcgcg gacggcaacg
     1141 atctgatcgg cttggcgccg cattggcaga gtctgctgcc cggggcggcg ttcgcagcgc
     1201 ccaatgcgcc gcagccttgt cccggcgcgg gctatcaatg gtttcctctt gcccggatcg
     1261 acccggaaga aatgcacaga ggcgtcgtcg ccgccgctgg tgcactggaa gccttccttt
     1321 cgcaggaact cgcccgtctg aagctgccgc cggatcgcct ggcgctggtg ggcttcagtc
     1381 agggcacgat gatggcgctg catgttggcc ttcgcggcgc ccttaagcct gccgcgatcg
     1441 tcggtttttc gggcttgctg acgggacctc cgtccgaaat caaagacgcg ccgccggtgc
     1501 tgctcggaca tggcgaggcc gacaatgtga tccctccgca ggcgctcttc gtcactgccg
     1561 cggcgctggg agccgccggg ctctgcgtgc agtggcatct ttcgccgggc cttccgcacg
     1621 gcattgacgc aactgaaatc gcgcttgccg gacgctttct ttccttggcc ttcggcggaa
     1681 agttgaaggc aagcggcgaa acgagctgcg ttctgggcgt cgccaatccg tcacagcgaa
     1741 tcgtataacg taacctgcgt attggcttgc gtcttggctt ttgcgggacg tgcccgtaaa
     1801 gtcggtcgta atagaggagg ctcggtcttc acggaatttt cgtcgtgtta gccgccgcgc
     1861 gcagccccga taactgcccg gctctggtcc tcaatgcgga ttttcgcccg ttgagctact
     1921 tccctttgtc gctgtggtcc tggcaggacg cgatcaaagc ggtgttcctg gaacgggtga
     1981 acattctcga ggaatatgac cgcaccgtgc actcgcccag cttcgagatg cgcctgccct
     2041 cggtcgtctc gctcaagacc tacatcaggc cggcgcgcaa tcccgctttc acgcgcttca
     2101 acgtctttct gcgcgaccgc ttcgagtgcc agtattgcgg cgccgacgag gacctcacct
     2161 tcgatcacgt catcccgcgc tcgcgcggcg gacgcacgac ttgggacaat gtggtaacgg
     2221 cctgcgcccc ttgcaatctg ctcaagggcg gaaaactgcc gcaccgcacc ggaatgcagc
     2281 cgcgtcatcg cccgcataag cccaccgtca acgaactgcg caacaacgga cgcttgttcc
     2341 cgccgaacta tctgcaccaa agctgggcgg attatctgta ttgggacagc gaactggaac
     2401 cgtaaaagcg aatagggttc ctgcccccgc cctgccaacc gaagctttag cgtaggtggg
     2461 gagcggggga agtggccgcg aagcggccga agggggctat tcgccatttc ggtcttcgtt
     2521 catatccgcg tggaaagcgc gatcgccaga cggcatcccg tcttgatggc gccgctgagc
     2581 aaggcataag cgggagcgcc ctcggcgcca tgggccagcg cggtgtcgcg atgagcgatc
     2641 tcgtcggcgc ggaatttctc gacggtctgt ttcaacgccg gatcgctgtc gcccaggcgc
     2701 tcgatctggc cggcataatg ccggtcgatc acctcttcca ccgcggcggt gcaggccatc
     2761 gccgcctttt cacccatcaa agcggtcgcc gcccccaggg caaagccggc gacgcgccag
     2821 accggctcca gtgccgtcgg gcgcacgcgc cgcccgttga tcagcgtatc gaaggccttc
     2881 agatgctctt gttcctgttc ggccatgtgc ctgatcgcgg cgacggtctt cgccttcgac
     2941 gcgtccgcgc cgaaaacggc aagctggcct tggtagattc gcaccgcgcc atactcgccg
     3001 gcatggtcga cgcggatcat ggcggcgatc tcgtccttgc cgccgacgcc gggcggcgca
     3061 gggcgcgggg cgcggcgatt tgccgtcact atcttctcct ccgcagcagg aacgtccgct
     3121 tgatcgccag aaaataagcg cccagggccg ccgcgccgag cgagaagatg gtgttgtagc
     3181 cggcgagcga aatgccgaac agcttccatg ccacgacatc gcagcgcacg acggctttgt
     3241 aattgacgag atccgccgcg ccgtggaact cgaagcgttc gccggtgcag gaggtcggtc
     3301 ccggaatgat cttccactcc acgcccgctt gccaggcgcc gatgatgccg gaggttgcaa
     3361 tcaggatcag cgcgatccag gccagcgccg tgatggtttg ccggccggcg atgccggcgg
     3421 cgaacaagcc cgcgccgccc aagcacagca cgatggccgc ttccaacggc cagcgctgcc
     3481 agtagcacat ctcgcaagga ggaacgccga caacgtactg gaagaattgc gtgcccagga
     3541 tcatcgccag ggagtagacg ccggccagga tgacggtttg gtttgcactc atggcgtgaa
     3601 aatcctcgtc ttgtcgtggc acactttgaa ttctcgcgcg ttgccgtgga cgagattata
     3661 cgctttatgt gcggtctcgt tcgggcttaa atgcgcaaca gcgccgagga aatcgaaacg
     3721 gaatcggggg cgagcgttca taggcggcct tgtgttgccg gcagaccaga gaagcaagat
     3781 acgcggcaaa agggaagccc atgcgtagaa aagaggcaac ccgcgctaaa gggcgtcggc
     3841 gcgtcgtaca tcgtgaattg ttgaaaatcg aaggccgggc cgtggaagtg agcgtggacc
     3901 tcaacccgag ggcgcggcgc ctgatcgtca aggttcatcc cgccagtggc gaggtcagcg
     3961 tggtcgcgcc gtcgaagacg gcgcttgagc gcgccctcga attcgcccgc ggccaaagcg
     4021 attggatcgc ccgccagctt gcccatgtgc cgagccgggt ggcgctggtg cccggcgcgc
     4081 atataccctt tcgcgggcgg gatcacttga tcgcgcgctg cgaggaggca cgcgttccgg
     4141 tatggatcga cgaaaccgcc gtggtcattc gcgtcggcgg cagagccgaa cataccccgc
     4201 gccgcctgct cgatttcctc aaacgtcagg cccgccgggc cttggaaact cgctcgatcg
     4261 aactggccgc caagctcggc ctcaggcccc ggcgcatcac cgtgcgcgac accgccagcc
     4321 gttggggctc ttgttcgtcg acgcgatcgc tgtcgttttc ctggcggctg attctggcgc
     4381 cggctttcgt cctcgattac gtcgtggcgc acgaagtggc gcacttgcgc gagatgaatc
     4441 acggggcgcg cttctggcgg ctggtgcacg aactggtgga cgatgtcgaa cggcctcagg
     4501 cttggctgcg ccagcacggc acgagcctgc accgctacgc ggcgaaataa acgcataata
     4561 cgttgaaatg gaaccggcgt tgctcaccct tcgacagtcg gtagcgacag cgtaccgacc
     4621 agggtgaggg tcaaagtcgt tttcctcatg ccgagcttgt ccgccttcgc atggctacgg
     4681 cggaccaaca gtacatcact cgcccgccgg agcttcagcg taggcgggtc gaagcatgac
     4741 gaaacaaccg cgaacaaatc aatctggcat caatctcagg ttccgctgaa cagccggtcg
     4801 agcaattgct gcaagccgtc gccttcttcg ggcggcggcg cggcggcggg cgcttgtccg
     4861 gccagcggcc ttgccggcaa tccgaactcg gcgtcttcca tgaaattctt gaagatgcgc
     4921 gccggcaggc cgccgcccgt ggcgcgccgc atcggggtat tgtcgtcgtt gccgacccac
     4981 accccgcaca ccagatcggc gctgaagccg acgaaccagg catcgcggta atcctgggtg
     5041 gtgccggtct tgccggcgct gggccggtcg gcgagccgcg cggccctgcc ggtgccggtg
     5101 gtcatcactt ccgtcatcag cgccgtcatc gccgcgagat tgtcggaaga catcacccgg
     5161 ccgagtccgg agcttttgcg ctgccacagg acttccccgt ctttcgtgcg catacggacg
     5221 attccgtagg gaagcacgcc gtcgccgccg ttggcgaacg gcgcataggc cgccgtcagc
     5281 tccagaggcg tcaccacgct ggtgccgagc gccagcgacg ataccgccat cagcggggtg
     5341 gcgatgccca gccgcttggc ggtgcgcgcc acggtcgccg ggcctacttc cgccgtcaat
     5401 tgcgccgcca ccgaattggc cgaatcggcg aaagccttgg tcaagctgat ctcgccttgg
     5461 aacttgcctt cgaaatcgct tggcctccag ccgttgatgt ttaccggcct gtcgttcatc
     5521 acatcggaag gcttgcggcc gtgctcgaag gcggcgagat agacgaaagg cttgaaggcc
     5581 gatccgggct ggcgctgggc gtcggcggcg cggttgtaag ggctttgttc gtaggacatt
     5641 ccgccgacca tggcgcgcac ggcgccgtcc ggcgtcatcg cgatcaaggc ggcctggcgc
     5701 gccctcagct ttgttccttc ttcgcgcaga ccggcagtga ccgcgcgttc ggcttcggtc
     5761 tgtgcgtcga gatcgaagct ggtatcgacg atcaaggcct tcttggcgtc gccgacatag
     5821 ccggggatct gcgagatcac ccagtcggcg aaatagcccg aaccgggcgt cgccgtgccg
     5881 cgcacgacgc gcgcgcgcgt ggcctgtgcg tcccggcggg cggcctcgtc gatgaagccg
     5941 gaatcctcca tgacgcgcag cacaatctgc gcgcgggcct ggctggcgtc cggatcggcg
     6001 agcggattgt agcgcgccgg cgccttgacg ctgcccgcca gcatggccgc ttcgggcaag
     6061 ctgagttttt ccgcgcgttt gccgaagaag cgctcggcgg ccgcttcgat gccgtaaacc
     6121 ccggcaccga aatagacccg gttgagatat aaggtgagga tctggtcctt cgagtaccgc
     6181 gactccagat agagcgcgag gatcgcctcc tggatcttgc ggtcgagggt gcgtccggat
     6241 tcgaggaaaa gattcttcgc caattgctgc gtcagcgtcg agccgccttg gacgacatgg
     6301 ccccgcatca tgttctcgac ggcggcgcgc gtcagcccga ccggatcgag gccgaaatgc
     6361 tcgcggaagc gccggtcctc gatggcgatg aaggcgttcg ccacataagg cggaagcttg
     6421 ccgacatcca ccatgattcc ctgggtcagg ccgcgccggg cgatcagtct gccgcgaacg
     6481 tccaaaatgg tgatatcgcg cgaaggaccg ttgaccagaa ggctgccgac atcgggcagg
     6541 tccgagagga aatgcgagaa gaaaatcgcc ccgaagatga cgctccacgc catcactagc
     6601 gtcacgacgt aaggccagct gcggcggcgc ctgggacgcg gcggctcact gccgccttcg
     6661 gcgatggcgg cgatgcgcgc gcgcgtgcgc cggtcgttct cggtttgcct gggcgccata
     6721 ggttaaccct tgaaagcgcg actctcgccc tcaagggtta acaggcatta aacaaccgcg
     6781 gcgcaatccg gatcagtcgc cgatcttgag gttcgacctg cccgtattgg acagccggcc
     6841 gcgataggcc ttgatcttga cgctacccga tcccagcgcg ctgatctcgg gatcgaccgc
     6901 catgccgcag aagc
//
LOCUS       DASWPU010000234         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_26179,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000234 DASWPU010000000
VERSION     DASWPU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26179"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      <1..103
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            205..>1855
                     /locus_tag="VGC27_05070"
     CDS             205..>1855
                     /locus_tag="VGC27_05070"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF006577.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEY0281975.1"
                     /translation="MPTLACDFDTHGHLIPAQEFALDRAAANPPRRAALRPAPLPAPL
                     ARPPQPANLILDRTRDDLLTTFGKATLSDRYLLPGEDFQDLFARVACAYADDAAHAQR
                     LYDAMSKLWFMPATPILSNGGTTRGLPISCFLNGVPDSLDGIVSTWNENVCLASNGGG
                     IGTYWGNVRSIGEQVKGCGETSGIIPFVHVMDGLTLAISQGSLRRGSAAVYLDVHHPE
                     IEEFLEIRKASGDFNRKALNLHHGIAVTDAFMEAVRDGRRFQLKSPKTGEVIRDVSAR
                     QLWQRILETRLQTGEPYLLFIDHVNRALPKHQCELGLKVQTSNLCSEITLPTGTDRSG
                     KERTAVCCLSSLNIETWDEWCDEPGFVEDVLRMLDNVLTSFVETAPDSMARAKYSAMR
                     ERSVGLGVMGFHSFLQARGIPMESALAKSWNLRLFRKIRREADAASVALAVERGPCPD
                     AAERGIKARFSHKLAIAPTASISIICGGTSACIEPIPANVYTHKTLSGAFVVRNPHLA
                     TLLAAKGADTPEIWQSIVEREGSVQHLDCLSDDEKAVFRTAF"
BASE COUNT          347 a          616 c          551 g          341 t
ORIGIN      
        1 cgagcttgcg ccacaaacgg ccactgatgc gttcgcatcg ggaaggccag gcaaaagcga
       61 caacccgcga gccaggagac ctgccgtgac ggtttgaatc gttcgtcggg cggggtgtcc
      121 cggtggccgc gtgctggcta tggcttcgag ccatcttccc gcgatgcgtc cgcttccgct
      181 cccgacgtca caggtggaga gacgatgcct acgctggcgt gcgattttga tactcatggc
      241 catctcatcc cggcacagga atttgcgctt gaccgggccg cagcgaatcc gcccaggcgc
      301 gccgccctcc ggccggcgcc gcttcccgct ccgctcgccc gtccgccaca gcccgccaat
      361 ctgatactcg atcggacgcg cgacgatttg ctgacgacct tcggcaaggc gacgctcagc
      421 gaccgctatc tgcttcccgg cgaagatttt caggatttgt ttgcacgcgt cgcctgtgcc
      481 tatgccgatg acgccgcgca tgcccagcgc ctctatgacg cgatgtccaa gctctggttc
      541 atgccggcga cgccgatcct ctccaacggc ggcacgacac gcggccttcc gatctcctgc
      601 ttcctcaacg gcgtacccga ttcactcgac ggcatcgtct ccacctggaa cgagaatgtc
      661 tgtctcgcct ccaacggagg cggcatcgga acctattggg gcaatgtcag gtccatcggc
      721 gaacaggtga aaggctgcgg ggagacatcc ggcatcattc cctttgtcca tgtgatggac
      781 gggctcacgc tcgccatcag ccaaggttcc ctccgtcgcg gttcggccgc cgtttatctc
      841 gacgtgcatc atccggaaat cgaagagttc cttgaaatcc gcaaagcctc cggcgacttc
      901 aaccggaaag ctttgaacct gcatcatggc atcgccgtca ccgacgcgtt catggaggcg
      961 gtgcgcgacg gccggcggtt ccaactcaaa agtcccaaaa ccggcgaggt gatccgagac
     1021 gtgagcgcgc gccagctctg gcaacgcatc ctggaaacgc ggctgcagac cggcgaaccc
     1081 tatctcctgt tcatagacca tgtgaatcgc gccttgccaa agcaccagtg cgaacttggg
     1141 ctaaaggtcc agacctccaa tctgtgcagc gagatcacgc ttcccacggg gaccgaccgc
     1201 agcggcaagg aacgcacggc ggtgtgctgt ctttcctcgc tcaacatcga gacttgggac
     1261 gagtggtgcg acgaacccgg gtttgtcgaa gacgtgctgc ggatgctgga caacgtgctg
     1321 accagttttg tcgaaacggc gcccgacagc atggcgcgcg ccaaatactc ggccatgcgc
     1381 gagcgctcgg tggggctcgg cgtcatgggg tttcattcgt tccttcaggc gcgcggcatc
     1441 ccgatggaaa gcgccctcgc aaaatcctgg aacctgcgcc tcttccgcaa aattcggcgc
     1501 gaggcggatg cggcgtcggt tgcattggcc gtggagcgcg ggccttgccc cgacgcggcc
     1561 gaacgcggca taaaggcccg cttcagccac aaattggcca tcgcccccac agcctccatc
     1621 agcattattt gcggcggcac cagcgcctgc atcgagccga tacccgccaa cgtctacacc
     1681 cacaaaacgc tgtcgggcgc ctttgtggtg cgcaaccccc atctggcgac gctgctggcc
     1741 gcgaagggtg cggacacgcc ggaaatctgg cagtccatcg tcgaacggga aggatcggtc
     1801 caacatttgg actgtctgag cgacgacgag aaggcggtat ttcgcacggc cttcg
//
LOCUS       DASWPU010000235         4140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_8068, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000235 DASWPU010000000
VERSION     DASWPU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8068"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGC27_05075"
     CDS             <1..193
                     /locus_tag="VGC27_05075"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY0281976.1"
                     /translation="GRDAALPGANTIIAIVATDADLTRIELKRLGHMAADCLARAIAR
                     GVYAALTLGDVKSYRDLFP"
     gene            complement(207..1274)
                     /locus_tag="VGC27_05080"
     CDS             complement(207..1274)
                     /locus_tag="VGC27_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281977.1"
                     /translation="MLFGDSGPAPNEGQSQTRPAYAPTQTSYSSVSSPSSGSLAPVAT
                     ITPVSIEPGSDTGTAVSKSVASLRSQIAGLQGSLAQNAQRLADLRNSGAEAASTYHEA
                     KARITTKLQIGTTRGNPELVSQWNVAQAALDQLAGNINGLSALGSAVAGDASTAHYVL
                     DQIAATFNVSGAVDEDHRQLSVLQDETNQTIVVIDRLLMEASESVQRQTAYVANERAN
                     LTVLNSAIKNGEFYGADIGAPLLTGSAASPSGNTFAYSGTPLVVIRFDRNNVEYQQIL
                     YTALSQALKTRPGAAFAVVAVSPTRGTTTAVQLAQTAARRHAQEVMRSMTDMGVLASR
                     LSVASATDPSATSSEVRVFVR"
     gene            1812..1888
                     /locus_tag="VGC27_05085"
     tRNA            1812..1888
                     /locus_tag="VGC27_05085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1846..1848,aa:Arg,seq:acg)
     gene            complement(2223..2462)
                     /locus_tag="VGC27_05090"
     CDS             complement(2223..2462)
                     /locus_tag="VGC27_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0281978.1"
                     /translation="MQKSLGAARHKALIDLLIQKREAAGMTQAELADRLGEYQSFVAR
                     LESGQRRVDVVEFLSLAEVLGFDPAKTISALTKVP"
     gene            2567..2893
                     /locus_tag="VGC27_05095"
     CDS             2567..2893
                     /locus_tag="VGC27_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281979.1"
                     /translation="MLRLAVIGIVTAPILGIPLWQVWDLIIRPRLIPIGEIKALADTM
                     IAKHGLRAEEMAFVEEDRAWRYSDAFEQGKWHRVRRELWRRYEAGKWKGPEEQPQSEN
                     PSDVSI"
     gene            complement(3232..>4140)
                     /locus_tag="VGC27_05100"
     CDS             complement(3232..>4140)
                     /locus_tag="VGC27_05100"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY0281980.1"
                     /translation="DTAFAVALIVMMGSRVPVELRIFLTAAAIVDDIGAIIVVAVFYS
                     GALEFGYLAAAAAIIVALALLNRSRIYSLAPYMLLGVALWACVHGSGLHATLAGVILA
                     LFVPTRSPPDFKALMIQANAILGAEAQHSGEVLRHGPSIPALRALEAIYDRLESPADR
                     LLRHAGARSSYIVLPLFALVNAGVVLTTDVVRGHEPLMLAIMIGLVIGKPLGLVLASA
                     LAVWLGLAVKPDEYSWRQLVGAGALAGIGFTMSLFIAGQAFPAEGDFAAAKIAVFAAS
                     VLSALVGVAVLWNAGYRADADRVAAS"
BASE COUNT          812 a         1250 c         1294 g          784 t
ORIGIN      
        1 ggggcgagat gccgccctgc cgggcgccaa cacgatcatc gccatcgttg ctaccgacgc
       61 agatttgaca cgcatcgaac tcaagcggct tggtcatatg gcggcggact gtctcgcgcg
      121 cgctatcgca cgcggcgttt atgccgcttt gacgcttggc gacgtcaaga gctatcggga
      181 cctcttcccc tgaacgggaa cgcggcttag cgcacgaaca cccgcacttc actggacgtt
      241 gccgacgggt cggttgccga ggcaaccgat agccgcgagg ccagcacgcc catatcggtc
      301 atgctgcgca tgacttcctg ggcatgacgg cgagcggctg tctgcgccag ttggaccgcg
      361 gtggtggtac cgcgcgtcgg cgatacggcg acgaccgcga aagctgcgcc gggacgggtc
      421 ttcaacgctt ggctcagcgc ggtataaagg atctgttgat actccacatt gttacggtcg
      481 aagcggatga cgaccagcgg cgtacccgaa taggcgaacg tgttgccgct gggactggcc
      541 gcagagccgg tcaacagggg cgcgccgatg tcggcaccgt agaattcgcc gtttttgatg
      601 gcactgttca acaccgtcag attggcgcgt tcgttggcga catacgccgt ctggcgctgc
      661 acgctttccg aagcttccat cagcagccgg tcgatcacga cgatggtctg gttggtttcg
      721 tcttgcagca ccgaaagctg gcgatggtct tcgtcgaccg cgccggacac attgaaggtc
      781 gcggcgatct ggtcgagcac gtaatgggcc gtggaggcgt cacccgccac tgcggagccg
      841 agtgcgctca gcccattgat attgccggcg agctggtcga gggccgcttg cgccacattc
      901 cattgactga cgagttccgg attgccgcgg gtggtaccga tctgaagctt ggttgtgatt
      961 cgggctttgg cctcgtgata ggtggacgcc gcctcggcgc ccgaattgcg caggtccgcg
     1021 agccgttgag cgttttgcgc cagagacccc tggaggccgg cgatctggct gcgcaggctg
     1081 gcgaccgact tgctgaccgc cgtgcccgta tcgcttcccg gctcgatgga aaccggcgtg
     1141 attgtcgcaa ccggggcaag gctgccgctg cttggtgagg atacgctgga ataggaggtc
     1201 tgggtgggtg cgtaagcagg acgcgtctgg ctctggcctt cgttcggggc tggacccgag
     1261 tcgccaaata gcatggagtc gacatctgcg cagcccgcca ggctcaaccc tgcgacgagc
     1321 actaaagcac tgaaaatgcg cgacgaaacg cgtgaagcct ctgaccgttc agtcatctta
     1381 tctgccatgt cccgctctcc gcatccgccg agccatccga atcggtgctt ggcgaaaaat
     1441 ccagcctgct cgcgcacttc ggttgcgagt gcccaaaaga agtcttaacc acgcaaacgg
     1501 gcggggacaa gagtgtccaa cgccgctttc tggggagttt tcctctgagt gcagggcctt
     1561 gaccgcccga tcgatccgtt cggctatgcc ggcccacaag ccccactgcg tacgccccga
     1621 acgcaagctg gcagggcact taagtatggt gccgataaag cacgctttgg ggcaaagacc
     1681 aagctgcgtc ccgtcggcgt cggccggtcc gtcaaccggg acgggtacag gggaccaggg
     1741 caagggaagc aggcgcgtta gcgggtcgct tgcctttgcc gcttgtggtg cataggttct
     1801 gccgccccaa agcaccggta gctcagctgg atagagcacc agactacgaa tctgggggcc
     1861 gcgggttcga atcctgcccg gtgcgccagt tcttttcgct cgcggcaagc tcgctttgtt
     1921 cgctgcctcc gcgacgacgg cgcacgaccg ccggggtgcg gtcgcgccca gggtttcccg
     1981 cgttctcagg ttcgctctac aaatttcgac aatgatttca atgtattaaa tgtgggcgat
     2041 agaggcagaa atgcttcggg tagcaccggg ctagcacgct ctgtggtttc caatttccca
     2101 ttgccgatgg gaatgcgggt ttcgtccgcg ccactcaatc cctctcgtgt caaaattaaa
     2161 gatagaggga caacgcttgg cgaagcggat cgcgaataca tcccccagca gtccccaaca
     2221 ggttacggca ccttcgtcag ggccgaaatg gtcttggccg gatcaaaccc gagcacttcc
     2281 gctaggctga gaaattccac aacgtcaaca cggcgttggc cgctttcaag ccgagcgacg
     2341 aaggactggt actcgccaag ccggtcagcg agttcagcct gcgtcatgcc cgccgcctct
     2401 ctcttctgga taagtaggtc gatcagagcc ttgtgacgtg ccgctcccag cgatttttgc
     2461 aattgcgccc cgcctctgtc gttgcgaggt cttcaaataa tctgaaattc agattatctt
     2521 aaaaatagat aaccctggaa cgataggaat ggatgctctt gacgatatgc tgcgattggc
     2581 tgtgataggg atcgtcaccg cccccatctt gggcatcccg ctctggcagg tctgggattt
     2641 gatcatccgg ccacggctga tcccgatagg tgagatcaag gcgctggctg acacgatgat
     2701 cgccaagcac ggcctacgcg cggaggagat ggccttcgtc gaagaggatc gggcgtggcg
     2761 ctattcggat gccttcgagc aaggcaagtg gcatcgggtg cgccgggagc tttggcgtcg
     2821 gtacgaggcc ggcaaatgga aggggccgga ggaacagcct caaagcgaga acccttccga
     2881 tgtttccatc tgaagggtcc gctcgtaaca ccatgggggt aggctatctc tttcgtcatc
     2941 cgcattgttg gggtttgcga tcttttgcct tggccattgc accgcgaagt tgacggttcc
     3001 tgttttgtag acaaagtgat gaaaggcggc gcttgtgctc ggcaatcatc cggtgcgccg
     3061 tgccagtggg cgcggtcacg cagaaatcgc tggcgttatg ccgcgacaga gaggtggtac
     3121 ggaatcaagc gcccttgttg ggaagcttca actttcggcc gcagtggttt cttccggcca
     3181 acaggcgggg gggccgctga atgttgttca gacggggacg ccgcacagcc tttatgacgc
     3241 agccacgcga tcagcatccg cgcgataccc ggcgttccac aacaccgcca cgccgacaag
     3301 ggccgacagc accgaggcgg cgaagacggc gatcttggcg gcggcgaaat cgccttctgc
     3361 aggaaaagcc tggccggcga tgaacaacga catcgtgaag ccgatgccgg ccaatgcgcc
     3421 ggcgccgacc agttgccgcc acgaatattc atccggcttg acggcgaggc ccagccaaac
     3481 cgcaagggcg gacgccaata ccagccccag cggcttgccg atcaccaacc caatcatgat
     3541 cgcaagcatc agcggctcat ggccccgcac gacatccgtt gtcagcacca cccccgcgtt
     3601 aaccagcgcg aacagcggca gcacgatata gctcgatcgc gctccggcat gtcgcagcaa
     3661 gcggtccgcc ggcgattcca gccggtcgta gatggcttcg agggctctta gcgcggggat
     3721 ggaaggtccg tgccgcaaga cctcgccgct atgctgggct tcggcgccca gaatcgcatt
     3781 cgcctggatc atcaacgcct taaaatctgg cggcgaccgt gtcggaacga acaacgccaa
     3841 gatcacgccg gccaaggtgg catgaaggcc gctcccatgc acgcaggccc acagcgcgac
     3901 gcccagcagc atgtagggcg ctaggctgta gatgcgcgaa cgattgagaa gcgccaacgc
     3961 cacgatgatc gcggcggccg cggcgagata gccgaactcc aaggcaccgg aatagaaaac
     4021 cgccacaacg atgatggcgc cgatgtcgtc gacgatcgca gccgcggtca ggaagatacg
     4081 cagttcgacc ggtacgcggc ttcccatcat cacgatcagt gcgaccgcga atgcggtgtc
//
LOCUS       DASWPU010000236         7869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_87298,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000236 DASWPU010000000
VERSION     DASWPU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87298"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VGC27_05105"
     CDS             <1..216
                     /locus_tag="VGC27_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281981.1"
                     /translation="RFALEDIPAEDNPACECGAILRGVKEPADCKLFGTVCTPETPTG
                     SCMVSAEGACAAHWTYGRFRDRARAAS"
     gene            213..1259
                     /gene="hypE"
                     /locus_tag="VGC27_05110"
     CDS             213..1259
                     /gene="hypE"
                     /locus_tag="VGC27_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEY0281982.1"
                     /translation="MSIKPYQRKLDLKNGRVDLSHGSGGRAMSQLIADIFHQALDNEW
                     LRRGNDQSAFDVAGSRMVMTTDGYVVTPIFFPGGDIGSLAVHGTINDVAMAGAQPLFL
                     SAGFIIEEGFALADLKRIAGSMGEAARAAGVPVITGDTKVVERGKADGVFVTTTGVGI
                     VPDGLDLSADKARPGDRVILSGSIGDHGVAVMSKRQNLTFETAIVSDSAPLHRLVAAM
                     VEAGGQALRVMRDPTRGGLAATLNELAHQSNIGFRLAEDAIPVKPEVTAACDLLGLDP
                     LYVANEGKLVAVVAPEAAEKVLAAMRTHPQGRDAAIIGEAVADERNFVQMKTGFGGYR
                     IIDWLSGEQLPRIC"
     gene            complement(1383..2762)
                     /locus_tag="VGC27_05115"
     CDS             complement(1383..2762)
                     /locus_tag="VGC27_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY0281983.1"
                     /translation="MSLDRSYFFCGIGGSGMLPLALILRTKGCTVAGSDRAFDQGRTP
                     EKFTFLRARGIALYPQDGGGVTDPGTVVVTSAAVESTVPDVEAARRIGARLMLRAELL
                     AQLFNAAPGSIGIAGTSGKSTTTGMAGWILSATGRDPTVMNGAVMKNFVTPDAPFASA
                     LVGNSDIFVSEVDESDGSIARYTPHIAVVNNVALDHKSLEELRGLFRDFAAKADVTVL
                     NLDNEETAALVTDKAITYSLTGRQARLQASRIVPAPDGIAFEVREGNKAAKVTLKVPG
                     RHNVSNALAALGAARASGVSLEKAARALSDFSGIKRRLEIVGSANGITVIDDFAHNPD
                     KIAATLATLHDFPGRLLILFQPHGYGPLKLMKDAFIEGFAQNLGGEDVLIMPEPVYFG
                     GTVERSVSSVDIARGVEARGLSAFAFADRAACGDKLIELANPGDRIVVMGARDDTLSQ
                     FAADLVGRM"
     gene            2821..3666
                     /locus_tag="VGC27_05120"
     CDS             2821..3666
                     /locus_tag="VGC27_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY0281984.1"
                     /translation="MRDGKVKIGVVAPASRLEPALAEMVQALARTLYADRVGIVFHPQ
                     CFLSWGHFAGDDETRAAAFLEVANDASFDALWVGRGGYGSGRIAGRVIAGLTQAAGKK
                     LYLGYSDAGALLGALYKHGFDGAAHGPMPADLLREGGEAAVARVLSFMVDGAAEALEP
                     TVSPATKTAAYNITVLSQLLGTPLQPDLADHILMLEDVSEHMYRIDRAMFHLTSNLAI
                     RKIAGIRLGRCSDIPPNDPDFGQSEEEVVHHWCGVSGISYLGRADIGHDADNKIVPFG
                     RLRTA"
     gene            complement(3667..5178)
                     /gene="hutH"
                     /locus_tag="VGC27_05125"
     CDS             complement(3667..5178)
                     /gene="hutH"
                     /locus_tag="VGC27_05125"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY0281985.1"
                     /translation="MNLDDPLTLAELRAVYQAPVRIELSPATLARIDAGAKAIAAIVA
                     GGATVYGVNTGFGLLASTSIARGELEALQRNLVLSHACGVGPLIPDAIVRLILMLKIA
                     SLSRGASGVRRQTVQMLARLAEAEIIPCIPSKGSVGASGDLAPLAHLAAALLGIGEVR
                     MDGAAVPAATALERAGLKPLVLGPKEGLALLNGTQVSTALALAGLFETEKVFAAGIVA
                     GAMSTDALKGSDAPFDARIHALRGQPGQIAVANALRALMTGSEIRESHRAPGADDKVQ
                     DPYSLRCQPQVMGAVLDIMRACARTLEIEASGVTDNPLVLDNGEVLSGGNFHAEPVAF
                     AADTLALALCEIGNISERRTAILVDPKMSGLPAFLVKESGLNSGFMIAQVTAAALVAE
                     NRMLAHPASVDTVPTSANQEDHVSMAAHGARRLLDMAGNAASVVAIELLAATQGLDFH
                     RPLKSSEPLEQAAHDIRSLVGPYERDRYFAPDIAAITGYVQDGALHKLVYGLL"
     gene            complement(5180..6052)
                     /locus_tag="VGC27_05130"
     CDS             complement(5180..6052)
                     /locus_tag="VGC27_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0281986.1"
                     /translation="MLALSAPGKRRLAYGFWRYREDEIGAAEEMLALARENDIDHLDT
                     ADVYGGPGGFGGSERLLGAVRASASHLFDGAVLATKIGIEPGSPYNSSPAYLTAACDA
                     SLSRLRIERIDLLYIHRPDLLTHPIELAQTLDGLVSSGKAAAIGVSNFTPAQVTALSR
                     HMKAPIAVHQLEFSAAHIDPLFDGTLDQAMAHGIAVAAWSPLAGGRLGGADATPTISR
                     IRETLERIGKHAGISPMAVALAFLHRHPAPITPILGTRNPARLRDCLKANGFTLSRAD
                     WYAIVEACRGERMP"
     gene            6225..7340
                     /locus_tag="VGC27_05135"
     CDS             6225..7340
                     /locus_tag="VGC27_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY0281987.1"
                     /translation="MQQFKLTRRAVAAACVVLALAGAGAAAQDTVPAKRNAILSYLHN
                     LQKQKQTLIGVQVNEYEVYLDCTSADRVFAQTGERPAIMGLELMNAIAYPPYAAYLTD
                     RALSQTAAGGLVTMSWHERNPVEICPRGEFYECAKKAMTAATLRAVLTKGTPENRLWL
                     ADVDAVAKVLGDLRKRGIVVLFRPYHEMNGGWFWWGKQDAFPELWDALYDELALRHKL
                     DNLIWVWSSDRDTPDAKRYFPLRHKPDIVGIDVYENDPDSPKYAAGRANVTALGGSAP
                     FALTEVGIIPSPKILDNLNPVWVLLWGGEYLNADWAWDNKCSGCNKPEAVSALFKLDR
                     TLSLAQLPAALRSTLAAGVVNQHPLHRKNPSCPAQLH"
     gene            7364..>7869
                     /locus_tag="VGC27_05140"
     CDS             7364..>7869
                     /locus_tag="VGC27_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HEY0281988.1"
                     /translation="MPTDFRSRLSALQAWHRALIERPNRPAEHGNGVYRRWANPILTA
                     DHTPLFWRYDLDPATNPFLMERIGVNATFNSGALYRDGKYLLVVRVEGADRKSFFAVA
                     ESATGVDRFLFWDYPVDLPQSGEPDTNVYDMRLTAHEDGWIYGLFCTERKDQVRPHDT
                     SAAVARCG"
BASE COUNT         1407 a         2503 c         2560 g         1399 t
ORIGIN      
        1 cgcttcgcct tggaagacat cccggccgag gacaatcccg cctgcgaatg cggcgccatc
       61 ctgcgcgggg tcaaagagcc ggccgactgc aagctgttcg ggaccgtctg cacgccggag
      121 acgccgacgg gttcctgcat ggtctccgcc gaaggcgcct gcgccgcgca ctggacctat
      181 ggccgtttcc gcgatcgcgc gcgggctgcg tcatgagtat caaaccctat caacgcaaac
      241 tcgatctcaa gaacggccgc gtcgatctct cgcacggctc cggcgggcgg gcgatgtctc
      301 agctcatcgc cgacatcttt caccaggccc tcgacaatga atggctcagg cgcggcaacg
      361 atcagtccgc cttcgatgtc gcggggagcc gcatggtgat gacgaccgac ggttacgtcg
      421 tgacgccgat cttcttcccc ggcggcgaca tcggttcgct cgccgtccac ggcacgatca
      481 acgatgtcgc catggcgggc gcccagccgc tgtttctctc cgccggcttc atcatcgaag
      541 aaggtttcgc gctcgccgac ctcaagcgca tcgccggcag catgggcgaa gcggcgcgcg
      601 ccgccggcgt tcccgtcatc accggcgaca ccaaggtggt cgagcgcggc aaggccgacg
      661 gtgtgttcgt caccaccaca ggcgtgggca tcgtccccga cggtctcgat ctctccgccg
      721 acaaggcgcg gccgggcgat cgcgtgatcc tgtcgggcag catcggcgat cacggcgtcg
      781 cggtgatgtc caagcggcag aacctcactt ttgaaaccgc catcgtgtcg gattcggcgc
      841 cgctgcatcg gctggtcgcg gcgatggtcg aagcgggcgg tcaggcgttg cgcgtgatgc
      901 gcgatccgac gcgcggcggc ctggcggcga cgctcaacga actggcgcat caatcgaaca
      961 tcggcttccg cctcgccgaa gacgccatcc cggtgaagcc ggaagtgacc gccgcctgcg
     1021 atctgctcgg gctcgatccg ctctatgtgg cgaacgaagg caagctcgtc gccgtcgtcg
     1081 ctcccgaggc ggcggaaaag gtgcttgcgg cgatgcgcac ccatccgcag gggcgcgatg
     1141 cggcgatcat cggcgaagcg gtcgccgacg agcgcaattt cgttcagatg aagacgggtt
     1201 tcggcggcta ccggattatc gactggctgt ccggcgagca actgccgcga atttgctgat
     1261 gcgattgttg ttcttatgct tcgacagtcg gtagcgacag cgtgccgacc agcatgagga
     1321 attaggcact ccctcaccct gagcttgtcg aagggtggag tgaacgcgtc agccaggcgt
     1381 gattacattc tcccgaccag atcggcggcg aattgcgaca gcgtgtcgtc gcgcgcgccc
     1441 atgaccacga tgcggtcgcc ggggttcgcc agctcgatga gcttgtcgcc gcaggccgca
     1501 cggtcggcaa aggcgaaggc ggaaagtccc cgcgcctcga ccccacgtgc gatatcgacg
     1561 ctcgacacgc tgcgctcgac cgtgccgccg aaatagaccg gctccggcat gatcaaaacg
     1621 tcctccccgc ccaggttctg tgcgaatccc tcgatgaaag catccttcat cagcttgagc
     1681 ggaccgtagc cgtgaggctg gaacaggatc agcagccggc ccggaaaatc gtgcagcgtc
     1741 gccagagtgg cagcgatctt gtcgggattg tgggcgaaat cgtcaatcac cgtaatgccg
     1801 ttcgcggaac ccacgatttc cagcctgcgc ttgatgccgg aaaaatcgct taacgcgcgc
     1861 gccgcttttt ccaggctcac accgcttgcc cgtgcggcgc ccagcgcggc cagcgcgttg
     1921 gaaacgttat ggcgccccgg cactttgagc gtcactttcg ccgccttgtt gccttcgcgc
     1981 acctcgaacg cgattccatc cggtgcgggc acaatcctgc tggcctggag ccgtgcctgg
     2041 cggccggtca agctataggt gatggctttg tccgtgacga gagcggcggt ctcttcattg
     2101 tccaggttca gcacggtgac atcggccttg gcggcgaaat cgcggaacag gccgcgcaac
     2161 tcctccaggg atttgtggtc caaggcgacg ttgttgacga cggcgatatg cggcgtgtag
     2221 cgcgcgatcg agccgtcgct ttcgtccact tcgctgacga agatgtcgct gttgccgaca
     2281 agggcgctgg cgaacggcgc gtccggggtg acgaaattct tcattacggc gccgttcatg
     2341 acggtcggat cgcgccccgt cgcggagaga atccagccgg ccatccccgt cgtggtggat
     2401 ttgccgctgg tgcccgcgat gccgatgctg cccggcgcgg cattgaacag ttgggccaag
     2461 agttctgcgc gcagcatcag cctggcgccg atgcgccgcg cggcttcgac gtcgggcact
     2521 gtgctctcga ccgctgccga agtgaccacg acggtcccgg gatcggtgac gccgccgccg
     2581 tcctgcggat aaagcgcgat gccgcgcgcc cgcaggaagg tgaatttctc gggcgtgcgc
     2641 ccctggtcga aggcgcggtc cgagccggcg accgtgcagc ctttagtccg caggatcaag
     2701 gcaagcggca gcatgccgct gccgccgatg ccgcagaaaa aataagacct atcaagactc
     2761 atggccgcac gttataaggc gggcaccgct gtgacaagcg attggaaaaa cacgcgaatt
     2821 atgcgcgacg gcaaagtgaa aatcggtgtg gtggcgccgg cttcgcgtct ggaaccggcc
     2881 ctggcggaaa tggtccaggc gctggcgcgc acgctctatg cggatcgcgt gggaatcgtc
     2941 tttcatccgc agtgctttct gtcatggggc cattttgccg gcgacgacga gacgcgcgcc
     3001 gcggcctttc tcgaggtggc gaacgatgcg agtttcgatg cgctgtgggt cgggcgcggc
     3061 ggttacggct cgggccgcat cgccggtcgg gtgatcgccg ggctgacgca agccgccgga
     3121 aagaagctct atctcggcta tagcgacgcc ggcgctctgt tgggcgctct ttataaacac
     3181 ggcttcgacg gcgcggcgca cgggccgatg cccgccgacc ttctgcgcga aggcggcgag
     3241 gcggcggtgg cgcgggtgct gtcttttatg gtcgacggcg ccgcggaggc gctggaaccc
     3301 acggtctcgc ccgcgaccaa aacggccgcc tacaacatca cggtcctcag ccaattgctc
     3361 ggtacgccgc tgcaacccga tttggcggac catatcctga tgctcgaaga cgtatcggag
     3421 catatgtacc gtatcgaccg cgcgatgttt cacctcacct ccaatcttgc catcaggaag
     3481 atcgccggga tcaggctggg ccgctgcagt gatattccgc ccaacgatcc cgacttcggc
     3541 cagagcgagg aagaggtggt gcaccattgg tgcggcgtgt cgggcatttc ttatctcggc
     3601 cgcgccgaca tcggccatga cgcggacaac aagatcgtcc cgttcgggcg cttgcgtacc
     3661 gcctgattac agcaatccgt aaaccagttt gtgcagcgcg ccgtcctgaa cataaccggt
     3721 gatcgcggcg atatccggcg cgaaatagcg gtcgcgttcg taaggtccca ccagcgagcg
     3781 gatgtcgtgc gccgcttgtt ccagcggctc ggagcttttc agcggacggt ggaagtcgag
     3841 gccctgggtt gcggcgagca attcgatcgc cacgacgctt gccgcgtttc cggccatgtc
     3901 gagcagccgg cgcgcgccgt gcgccgccat cgagacatga tcttcttggt tcgcgctggt
     3961 cggcaccgta tcgaccgaag cggggtgcgc cagcatccgg ttctccgcca ccagggccgc
     4021 ggcggtgacc tgcgcgatca tgaacccgga attgaggccc gactccttca ccaggaaggc
     4081 gggcaatccg ctcattttgg gatcgaccag aatggcggtg cgccgctcgg aaatattgcc
     4141 gatctcgcac aacgccagcg ccagcgtgtc ggcggcaaac gccaccggct cggcgtggaa
     4201 attcccgccg gagagaactt cgccattgtc cagcaccagc ggattgtcgg tgacgcccga
     4261 ggcttcgatc tccaaggtgc gggcgcaggc gcgcatgata tcgagcacgg cgcccatcac
     4321 ttgcggctgg cagcgcaggg aataggggtc ctgcaccttg tcgtcggcgc cgggcgcgcg
     4381 atgggattcg cggatctcgc ttcctgtcat caaggcgcgc agagcgtttg cgaccgcgat
     4441 ctggccgggc tggccgcgca gcgcatggat gcgcgcgtcg aacggcgcgt cggaaccttt
     4501 aagcgcatcg gtggacatgg cgcccgcgac gattcccgcc gcgaacacct tctcggtttc
     4561 gaacaagccg gccagcgcca gcgcggtgga aacctgggtg ccattgagca gagcgaggcc
     4621 ctctttcggt cccaacacca gcggcttgag gcctgcgcgc tccagggcag tcgcggccgg
     4681 gacggcggca ccatccatgc gcacctcgcc gattcccagc agcgccgccg ccagatgcgc
     4741 cagcggcgcg agatcgcccg aggcgccgac cgagcctttg gaaggaatgc agggaatgat
     4801 ttccgcttcc gccaggcggg cgagcatctg cacggtttgg cgccgcacgc ccgaggcccc
     4861 gcgcgacagc gaggcgatct tgagcatcag gatcagacgg acgatggcgt cggggatcaa
     4921 tgggccgacg ccgcaggcat ggctgagaac aagattgcgc tgcaacgctt cgagttcgcc
     4981 gcgcgcgatc gaggtgctgg cgaggaggcc aaatccggta ttgacgccgt agaccgtggc
     5041 gccgccggcc acgatggcgg cgatggcctt ggcgccggca tcgatgcgcg ccagcgtcgc
     5101 cggggaaagt tcgatccgca cgggggcttg gtaaaccgcg cgcaactcgg caagcgtgag
     5161 aggatcgtcg agattcatgt cagggcattc tttcaccgcg gcaggcttcg acgatggcat
     5221 accagtccgc ccgcgacagg gtaaagccgt tcgcctttaa acaatcgcgc agccgggccg
     5281 gattcctggt gccgaggatc ggcgtgatcg gtgcggggtg gcggtgcaag aaggcgagcg
     5341 ccacggccat aggcgaaata ccggcgtgct tgccgattct ttcgagcgtt tcgcgtatgc
     5401 gggaaattgt tggcgtggcg tccgctccgc ccagccgtcc gcccgccagc ggcgaccacg
     5461 ccgcaaccgc tatgccatgc gccatcgcct gatccagcgt gccgtcgaac aagggatcga
     5521 tatgagccgc ggagaattcc aactggtgga cggcaatcgg cgctttcata tgtcgggaaa
     5581 gcgcggtgac ctgagccggc gtgaagttcg aaacgccaat ggcggcggcc tttcccgacg
     5641 agaccaagcc gtccaaggtc tgcgccagct cgattgggtg cgtcagcagg tctgggcgat
     5701 gtatataaag taaatcgatc cgctcgatcc gcaggcgcga cagactcgcg tcgcaagcgg
     5761 cggtcaaata ggccggcgag gaattgtaag gcgagcccgg ctcgatgccg atcttggtgg
     5821 cgaggaccgc gccatcgaac aagtgcgacg cgctggcgcg aaccgcgccg agaagccgtt
     5881 cggagccgcc gaagccgccg ggtccgccat aaacgtcggc cgtgtcgagg tggtcgatat
     5941 cgttttcccg cgccagcgcg agcatttcct ccgcggcgcc gatttcatct tcgcgatagc
     6001 gccagaagcc ataggcaagc cgccgctttc cgggagccga taaggcgagc attggctttc
     6061 ctcttgaatg tcgtcgacaa aaggccggga aggacaggcc atctttaatc gcagactggc
     6121 aacgattcca ccgatgacag cgctgccata ctctcgcaat tcctctataa tcgaaggcgt
     6181 cggcttcgca tagccggtac aagggtgaga tgatggagtc ggttatgcaa cagttcaagc
     6241 tgacaaggcg cgctgtggcc gccgcttgcg tcgttctggc gttggcgggt gcaggcgcgg
     6301 cggcgcagga cacggtcccg gcgaaacgga atgcgattct ctcctatctg cacaaccttc
     6361 agaagcaaaa gcagactctc atcggcgtac aggtgaacga gtacgaagtc tatctcgact
     6421 gtacttcggc cgaccgtgtt ttcgcgcaga ccggggaacg cccggccatc atgggcctgg
     6481 agttgatgaa cgcgatcgcc tatccgcctt atgccgccta tttgaccgac cgcgccttga
     6541 gccaaaccgc tgccggcggg ctggtcacca tgtcgtggca cgagcgcaac ccggtggaga
     6601 tctgcccgcg cggcgagttc tacgaatgcg ccaagaaagc gatgacggcg gccacgctgc
     6661 gcgccgtgct caccaagggt acgcccgaaa accggctgtg gctggcggac gttgacgccg
     6721 tcgccaaggt tctgggcgat ttacgcaagc gcggcatcgt tgtcctgttc cggccttacc
     6781 acgaaatgaa cggcggctgg ttttggtggg gaaagcaaga cgccttcccg gagttgtggg
     6841 acgcgcttta tgacgagctg gcgcttcgcc ataagctcga caatctgatc tgggtgtggt
     6901 cctcggaccg cgatacgccc gatgcgaagc gctatttccc cttgcggcat aaaccggaca
     6961 tcgtgggcat cgacgtctac gaaaacgacc cggacagtcc gaaatacgcc gccggccgcg
     7021 ccaatgtgac ggcgcttggc ggatcggcgc cgtttgctct taccgaagtc gggatcatcc
     7081 ccagccccaa gattctggat aatctcaacc cggtatgggt gctgctatgg ggcggcgaat
     7141 atctgaatgc ggattgggca tgggacaata aatgctcggg ctgcaacaag cccgaagccg
     7201 tgtccgcgct gttcaagctc gaccgcacgc tgtcgctcgc tcaactgccg gcggcgctgc
     7261 gctccacgct cgcggccggg gtcgtcaacc agcatccttt gcatcgaaag aacccgtcct
     7321 gtccggcgca gttgcattga acggcttcga ggagcgagcg accatgccga ccgatttccg
     7381 gagccgcctt agcgcattgc aggcttggca ccgtgccttg atcgagcggc ccaaccgccc
     7441 cgccgagcac ggcaacggcg tctaccggcg ctgggcgaat cccatcctga cggccgatca
     7501 cacgcccttg ttctggcgat acgatctgga ccccgccacc aatccgttcc tgatggagcg
     7561 gatcggcgtc aatgccacct tcaattccgg cgccctttat cgcgacggaa aatatcttct
     7621 ggtggtccgg gtggaaggag cggaccgcaa atccttcttc gccgttgccg aaagcgccac
     7681 cggcgtcgac cgctttttgt tctgggatta tccggtggat ttgccgcaaa gcggcgagcc
     7741 ggacaccaac gtctacgaca tgcgcctgac ggcgcacgaa gacggctgga tctacggtct
     7801 cttctgcacc gagcggaaag accaggtgcg gccgcacgac acctccgcgg ccgttgcgcg
     7861 gtgcggcat
//
LOCUS       DASWPU010000237         4648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_38965,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000237 DASWPU010000000
VERSION     DASWPU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38965"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1541)
                     /gene="gatA"
                     /locus_tag="VGC27_05145"
     CDS             complement(51..1541)
                     /gene="gatA"
                     /locus_tag="VGC27_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY0281989.1"
                     /translation="MSLPAVPTDFTLTEARDAVAAKKISSRELTQAFVKAIEAARPLN
                     AFITETPERALEMAAASDARLAKGEGGPLEGLPLAIKDLYCTKGVRTTAASKILSNFV
                     PPYESTVTQNLWNAGAVMLGKANLDEFAMGSSTETSWFGPTVSPWRAKDSVAKMVPGG
                     SSGGSAAAVAANLCLAATGTDTGGSIRQPAAITGTVGIKPTYGRCSRWGIVAFASSHD
                     QAGPMTRTVRDAAILLRVMASVDPKDSTSVDVPVPDYEAGLDSGVKGLRVGLPKEYRV
                     DGAPPEIDAIWSKGAEWLRAQGAEIVEVSLPHTKYALPTYYIIAPAEASSNLARYDGV
                     KYGHRAKDASELLDLYERSRGEGFGAEVRRRILIGTYVLSAGYYDAYYARAQKVRSLI
                     ARDFTKAFEQCDVLLTPVTPGPAFAFGANSDDPVAMYLNDVFTVTLNLAGLPGLVVPA
                     GLTAEGLPLGLQLIGKAFDEATVLRAGRAVETAANFTAKPQPWWVK"
     gene            complement(1541..1828)
                     /gene="gatC"
                     /locus_tag="VGC27_05150"
     CDS             complement(1541..1828)
                     /gene="gatC"
                     /locus_tag="VGC27_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY0281990.1"
                     /translation="MSVDKDTVRRIARLARLALEEERVEPMVQELNGILAWVEQLKEV
                     DIEGVAPLTSVVEQRLKMREDVVTEGNNADALMINAPGGEDHFFVVPKVVE"
     gene            1935..2408
                     /gene="ruvX"
                     /locus_tag="VGC27_05155"
     CDS             1935..2408
                     /gene="ruvX"
                     /locus_tag="VGC27_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY0281991.1"
                     /translation="MSIADLSELRSLLPPGSRLLGLDLGEKTIGLALSDTTLTVATPM
                     ETLKRGKFTTDAAVLEKIVAEQGVGGLVIGLPLNMDGSEGPSAHSARAFARNFAARSA
                     IPIALCDERLSTAAVTRTLLEADASRQRRAALVDKMAAAYILQGALDRLKRLGGK"
     gene            2405..3715
                     /locus_tag="VGC27_05160"
     CDS             2405..3715
                     /locus_tag="VGC27_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0281992.1"
                     /translation="MNVTADDRPTKIGWLPPGLRPPGGMTIRQERVFLLVGLAALFAG
                     YDMNVFGMAIPQIQASLHIPEDKVGLTVSFFRMAAIAAMLLAWSADLVGRRRLLLFTI
                     FGQAVATLGTAFAQTYEQFVWLQIATRVFGYAEEMLCFVVIAEEVAASLRGWANGTLA
                     AMNYAGAGLASIVFATITFLPGGWRGMYVIGALPLFLVAYLRRSLPETRRFEAREKEV
                     QKLSSRIATALDVTRRLAKEHPRRLATILIVVAAFGFAIWPACILGPKYLQDTLHFTP
                     LQTTLLIVPGGLVAVVFNILAGRLSDRIGRRRVVFGACFVAVASYGTFYSGIHWPYVA
                     TMWVPGFFGFLCADALIAGLSAEIFPTAYRATVSGLRYVVGILAGAAALALEGPLYNL
                     FHAHGPALIVLMAAMPIAMIAVLRLPEPAGRTLEDIASEPDRRS"
     gene            3749..4135
                     /locus_tag="VGC27_05165"
     CDS             3749..4135
                     /locus_tag="VGC27_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020011479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HEY0281993.1"
                     /translation="MTKLSSDDKYDPDGSLQRLLDLNGEIMDMGDGHWVKFNVHRIEP
                     DEKRPHGIAYSLCLFSPDDERLVCFNNAHSVATGSCPSKKQTEISDHVHVGEQIKPYA
                     YSDAEELMSDFWQAVETVLSAKGVQS"
     gene            4132..4503
                     /locus_tag="VGC27_05170"
     CDS             4132..4503
                     /locus_tag="VGC27_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0281994.1"
                     /translation="MNVLKIGIASREEMKSRTMAVARGELKPNRDDPKVWFTSIESLA
                     QVLSTKNKMLLEIIKKAKPTSLAELAKLSEREESNLSRTLHTMERYGFVILQKTEKGR
                     LVVIAPYDAYEVKYGFAKAVA"
BASE COUNT          855 a         1479 c         1452 g          862 t
ORIGIN      
        1 gggtcgaacc cggccatgac gatataggtc ataatcagag accgaaggca ttatttgacc
       61 caccagggct gcggcttggc ggtgaaattc gcggcggttt cgaccgcgcg tccggcgcgc
      121 agaacggtgg cctcgtcgaa cgccttgccg atgagctgca gccccagcgg caagccttcc
      181 gccgtcagcc cggccggcac cacaagaccc ggcaggccgg ccaggttcag cgtcaccgtg
      241 aagacgtcgt tcagatacat cgccaccgga tcgtcggaat tcgccccgaa ggcgaaggcc
      301 gggccgggcg tgaccggcgt cagcagcacg tcgcactgct cgaacgcctt ggtgaaatcg
      361 cgcgcgatca gcgagcgcac cttctgggcg cgggcgtaat aggcgtcgta atagccggcc
      421 gaaagcacat aggtgccgat cagaatgcgc cggcggacct cagccccaaa gccctcgccg
      481 cggcttctct cataaagatc aagcagttcg ctcgcgtcct ttgcccggtg gccgtatttc
      541 acgccgtcat agcgggcgag gttcgaggac gcctccgccg gagcgataat gtaataagtg
      601 ggcagcgcat acttggtgtg cggcaggctg acctcgacga tctcggcgcc ctgggcacgc
      661 agccattcgg cgcccttgct ccagatggcg tcgatttccg gcggcgcgcc gtccacgcgg
      721 tattccttgg gaaggccaac gcgcagcccc ttcacgccag agtccagccc cgcctcgtaa
      781 tcgggcaccg ggacatcgac gctggtggaa tccttgggat ccacgctggc catcaccctc
      841 agcaggatcg cggcgtcgcg caccgtgcgc gtcatcggtc ccgcctggtc gtgcgacgaa
      901 gcgaaggcaa cgatgcccca gcgcgagcag cgcccatagg tcggcttgat gccgacggtt
      961 ccggttatgg cggcaggctg gcggatcgat ccgccggtgt cggttccggt cgccgccaga
     1021 cacaggttgg ccgcgaccgc cgccgccgaa ccgcccgacg aaccgccggg caccatcttt
     1081 gcgacggaat ccttcgcccg ccacgggctg accgtcgggc cgaaccagct tgtctcggtc
     1141 gacgagccca tcgcgaactc gtccagattg gccttgccga gcatcaccgc gcccgcattc
     1201 cacagattct gcgtcaccgt ggattcgtaa ggcggcacga agttcgagag gatcttgctg
     1261 gccgccgtgg tgcgcacgcc cttggtgcag tacagatcct tgatcgccag cggcagccct
     1321 tcgagcggcc cgccttcgcc tttggcgagg cgggcgtcgg aggccgccgc catctcaagg
     1381 gcgcgttcgg gagtctcggt gatgaaggcg ttgagcgggc gcgccgcctc gatggccttg
     1441 acgaaggcct gagtcagctc gcgcgaagaa atctttttag cggcgacggc atcgcgtgct
     1501 tcggtcaaag tgaaatcggt ggggacggcg ggaagagaca ttattccacc accttcggca
     1561 cgacgaagaa gtggtcctcg ccgcccgggg cgttgatcat caacgcatcg gcgttgttgc
     1621 cttcggtcac cacgtcctcg cgcatcttca ggcgctgctc gaccacgctg gtcagcggcg
     1681 cgacgccctc gatatccact tccttgagct gttccaccca ggccaggatg ccgtttagct
     1741 cctgaaccat gggctccacg cgctcttctt ccagcgccag ccgggcgagc cgggcgatac
     1801 gccggacggt gtccttatcg acggacatgg ggtctatgcc gtgctaaata cgagccacct
     1861 tgctagcaac cccaaggccg ggctggcaag gacgatggag ccgcatcgcg gcaccgttct
     1921 gcgaccgagg gacgatgagc atagccgacc tgtccgaatt gcgcagcttg ctccccccgg
     1981 ggagccggct tcttggcctc gatctcggcg aaaagacgat cgggctggcg ctgtcggaca
     2041 cgacgctgac cgtggcgacg ccgatggaga cgctgaaacg gggtaagttc acgaccgacg
     2101 ccgcggtgct ggagaagatc gtcgccgagc aaggggttgg ggggctggtg atcggcctgc
     2161 cgctcaacat ggacggcagc gagggaccct cggcccactc cgcccgcgcc tttgcgcgca
     2221 atttcgcggc gcgttccgcc attcccatcg cgctgtgcga cgaacggctt tccaccgccg
     2281 cggtgacgcg gacgctgctg gaagcggatg cttcacgcca gcggcgcgcc gctctcgtgg
     2341 acaaaatggc ggccgcctat atcctgcaag gcgcgctcga ccgcttgaag cgcctggggg
     2401 gaaaatgaac gtgacggccg acgaccgtcc cacgaaaata ggctggctgc cgccgggtct
     2461 gcgcccgccg ggcggcatga cgatccggca agagcgcgtg ttccttctcg tcggcctggc
     2521 cgctttgttc gccggctacg atatgaacgt gttcgggatg gccatcccgc aaatccaggc
     2581 cagcctgcac atccccgaag acaaagtcgg actgacggtc tccttcttcc gcatggccgc
     2641 aatcgccgcg atgctgctgg cgtggtcggc cgatctcgtc gggcggcggc gtctgttgct
     2701 gttcacgatc ttcggccagg cggtggccac gctcggaacc gccttcgccc agacctacga
     2761 gcagttcgtc tggttgcaga tcgccacgcg cgtgttcggc tatgccgagg aaatgctctg
     2821 cttcgtcgtc atcgccgaag aagtcgcggc cagcctgcgc ggctgggcga acggaaccct
     2881 ggcggcgatg aattatgccg gcgccggact ggcttcgatc gtattcgcca cgatcacctt
     2941 cctgcccggc ggctggcgcg ggatgtatgt gatcggtgcg ctgccgctgt tcctagtggc
     3001 ctatctgcgc cgcagcctgc ccgaaacaag gcgcttcgaa gcgcgcgaaa aggaggtcca
     3061 gaagctatcc tcgcgcatcg ccacggcgct ggacgtgacg cgccgcctcg ccaaggaaca
     3121 tccccgccgc ctcgccacga ttttgatcgt cgtggccgcc ttcggtttcg caatctggcc
     3181 ggcttgcatt ctcggcccca aatatctcca ggacacgctg cacttcacgc cgttgcagac
     3241 gacgctgctg atcgtgcccg gcggactggt ggcggtcgtc ttcaacatcc tggcgggacg
     3301 gctcagcgac cggatcggcc gacggcgcgt cgtcttcggc gcctgcttcg tggcggtggc
     3361 cagctacggc acgttttaca gcggcatcca ctggccttac gtcgctacca tgtgggtgcc
     3421 cggctttttc ggctttctct gcgccgatgc gctgatcgcg ggcctctccg ccgagatatt
     3481 cccgaccgcc tatcgcgcca cggtttcggg cttgcgctac gtggtgggga ttctggccgg
     3541 cgccgccgcc ctggcgctgg aaggcccgct ctacaacctg ttccatgccc acggtccggc
     3601 gctcatcgtc ttgatggcgg cgatgccgat cgcgatgatc gcggtcctgc gcctgcccga
     3661 accggcgggg cgcaccctgg aagacatcgc aagcgagccg gacaggagaa gttaatatat
     3721 tgaaaatgca gagatattgg agaaatcctt gacaaagtta tcatccgatg ataagtacga
     3781 tcctgatgga agcttgcagc ggcttctcga tctcaatggc gagatcatgg atatgggaga
     3841 cggccattgg gtgaaattca acgttcacag aattgagccg gacgaaaaac gccctcacgg
     3901 aattgcttac agcctctgct tgttttcgcc cgatgatgaa aggctcgtct gtttcaacaa
     3961 tgcacattct gttgccaccg gcagctgccc ctccaaaaaa cagacggaaa tcagcgacca
     4021 cgtgcatgta ggcgaacaaa tcaagccata cgcctattcc gatgcagaag aattgatgtc
     4081 cgatttctgg caagccgttg aaaccgttct gagcgcgaaa ggagtgcagt catgaatgtc
     4141 ttgaagatcg gcattgcctc gcgcgaagag atgaagtccc gcacgatggc ggtggcacgg
     4201 ggcgaactga aaccgaacag ggacgatccg aaagtctggt tcacctcgat tgagtcactg
     4261 gcacaggttc tttcgaccaa gaacaagatg ctgctggaga tcatcaagaa ggccaagccg
     4321 acctctctgg cggaattggc gaagctctcc gagcgggagg aaagcaactt gtcccggacg
     4381 cttcacacga tggagcggta tggatttgtc attcttcaaa aaaccgagaa aggcagatta
     4441 gtcgtcatag cgccttatga tgcttacgaa gtgaaatacg gcttcgcaaa ggccgttgcg
     4501 taaggcactg cgcaccatcg gaaaatcgca aaccgcgggg aacgccatga gcactcttat
     4561 gaagttccgc tgggtgatca tcttcgttgt ggccattgcc ctgtgcgtcg cggcggcgaa
     4621 catcatcgag cacccgagtt cgtcccca
//
LOCUS       DASWPU010000238         4144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_112296,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000238 DASWPU010000000
VERSION     DASWPU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112296"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VGC27_05175"
     CDS             complement(<1..797)
                     /locus_tag="VGC27_05175"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEY0281995.1"
                     /translation="MRDRLISILIGAAIAALISLPAKADDPNWVVGPQQRFDVKSLKI
                     ENILGIVKIDVRDGGQTTLQINGIRQRVDAISVKETDGTLSIRGRAERSVWDWNHWFD
                     FSYNARKADQLYVLLTVPKRTRVSVNGIIGKATIGNTEGEIRFSSAGRTESTIGNVTE
                     AKISLAGSGKISIGNVAGTLRAETAGSGSIKAGDAGKVNADIAGSGSILVGRVNGPLK
                     VDIAGSGDFTAASVYGPVKTDIAGSGSVTIAGGEANPLNVDIMGSGNV"
     gene            complement(920..3223)
                     /locus_tag="VGC27_05180"
     CDS             complement(920..3223)
                     /locus_tag="VGC27_05180"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0281996.1"
                     /translation="MPGANSLTSTLREALGLHHAGRLAEAEAVYRRILSSQPRNFDAL
                     HFLGVLRAQGGDMTGAVALIGKALEINPNSPLAHFHIAGAYAGLGRAAKALASYERAV
                     ALKPDFAEAHSARARILLDIGHAAQAVAACERAIAANPNIADTHLIRGNALLVLDRTE
                     EALNALDRTIMLSPGSAEAYYNRGNTLVTLERNEEALAAFGRAIALKPDYAEALNNRG
                     DVLCQLGRYKEALADCERSVALAPGKSPGYHNMGNALMALERAAEAMAAYDRAIALDP
                     NFAKPYYGRVGLWRDLGRFEEALADAEKALALDPANGRAACEAFYATGVLCDWRDRAS
                     RVDDLVRRCREGQLLYPWPLLSAFDDPELHRIAAKKFGGSALSGTLAAAGSSERLRVA
                     YLSADFHDHAVSYQVVELFERHDRTRLETYGICLRSGSDTPMRRRLRHAFDHFIELGA
                     RSDREVAQTLADLHIGIVVDLNGYTGKSRSKMLSLRPAPIAVNYLGFPGTLGTNYVDY
                     IIADARVIPVGSESYYSENVVRLPDCYLPSDTRDRGDPEPPSRSAAGLPEQGFVFCVF
                     NNAYKITPGMFDIWMRLLRKVDGSVLWLSVSNPAARDNLRAEAEARGVSPGRLIFAGY
                     TEERAQHLARIALADLFLDTLPYNAHSTASDMLWAGVPVVTCMGRSFASRVAGSMLSA
                     IGAEELITHDLGAYEALAFDLARSPERLAAVRGKLACSRASHPLFDTARLCRQLEAAY
                     ETMWDFHLKGEKPQSFTVNILAEPSPS"
     gene            complement(3239..3922)
                     /locus_tag="VGC27_05185"
     CDS             complement(3239..3922)
                     /locus_tag="VGC27_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0281997.1"
                     /translation="MSSAKRVLLVEDDAMLRASLAEQLSQEGTYTVVEAQDCAAGRLA
                     AGDGLYEFMILDVGLPDGDGRELCQELRSQGVTCPILLLTAADTDADTIAGLRSGAND
                     YVTKPFRFAVLMARVHAHLRSHGRSEEAMYRIGPYTFRPSAKMLLDADARKIRLTEKE
                     TNILKYLYRCGDTVPRETLLHEVWGYNPAVTTHTLETHIYRLRQKIELNPGEARILVT
                     ESGGYRLMA"
BASE COUNT          759 a         1306 c         1313 g          766 t
ORIGIN      
        1 acattgcccg agcccatgat gtcgacattc agcggattgg cctcgccgcc ggcgatggtg
       61 acggaacccg aaccggcgat atccgtcttg accggcccgt aaacgctggc ggccgtgaaa
      121 tcgcccgatc cggcgatgtc caccttcagg gggccgttga cccggccgac taggatcgag
      181 cccgatccgg cgatgtcggc gttcaccttg cccgcgtcgc cggccttgat gctgccgctg
      241 cctgcggttt cggcacgaag cgtcccggcg acattgccga tacttatctt gccggaaccg
      301 gccagcgaga ttttcgcttc tgtgacgttg ccgatcgtcg attccgttct gccggcggag
      361 gaaaagcgta tctcgccttc ggtgttgccg atggtggctt tgccgataat accgttgacg
      421 gaaacgcgcg ttctcttggg aaccgtcaga aggacataga gctgatcggc cttcctggca
      481 ttgtacgaaa agtcgaacca atggttccaa tcccacaccg atcgctcggc acggcctctg
      541 atcgacaagg tgccgtccgt ctccttgacg gaaattgcgt ccacccgctg cctaattccg
      601 ttgatctgaa gcgtcgtctg gccgccgtcc cgcacgtcga tcttcacgat gcccaagata
      661 ttttcgatct tgagcgattt gacgtcgaac cgctgttgcg gcccgacgac ccagttggga
      721 tcgtcagcct tcgccggcaa ggaaatcagg gcggcaatcg ccgcgccgat cagaatggaa
      781 atgagccgat cgcgcatggt ctctccgggg aacctcgtgt caggttacaa atagtctgct
      841 gccactgtta ggcacagata gtagattttg cagaacctgg tcccaatggc agaaggggaa
      901 caaaatagag acgccccggt caggaaggac tgggttccgc cagtatatta accgtaaaac
      961 tctgcggttt ttctcccttc aggtgaaaat cccacatggt ttcgtaggct gcctcgagct
     1021 ggcggcaaag acgcgcggta tcgaacaggg gatggctggc ccggctgcag gcgagcttgc
     1081 cgcggacggc ggccagccgc tccgggctgc gcgccagatc gaacgcaagc gcttcatagg
     1141 cgccgagatc gtgcgtgatc agctcctcgg cgccgatggc ggacagcatg ctgcccgcta
     1201 cacgcgaggc gaagctccgg cccatgcagg tcactaccgg caccccggcc cacaacatgt
     1261 cgctggcggt tgagtgcgcg ttatagggca gcgtgtccag gaacaaatcc gccaaggcga
     1321 tgcgggccag atgttgcgca cgctcttcgg tgtaaccggc aaatatgagc cggcccggag
     1381 aaacaccacg cgcctcggct tcggcgcgca gattgtctct tgccgccgga ttgctgacgc
     1441 tcagccatag aacgctgccg tccaccttgc gcaacaagcg catccagata tcgaacattc
     1501 ccggcgtgat cttgtaagcg ttgttgaaga cgcaaaagac gaagccttgt tccggcaagc
     1561 ccgccgcaga gcgcgagggc ggttcgggat cgccacggtc ccgggtgtcg ctcggcagat
     1621 aacaatccgg caggcgtacc acattctccg aatagtacga ctcggagccg acggggatca
     1681 cccgagcatc ggcgatgatg tagtcgacat agttcgtacc cagcgttcct ggaaagccca
     1741 gataattgac ggcgatcggc gcgggacgca gggacagcat tttcgaccgg ctcttgccgg
     1801 tatagccgtt gagatcgacg acgatgccga tatgcagatc ggcgagcgtc tgggcgacct
     1861 cgcggtcgga acgcgcccca agttcgataa aatgatcgaa cgcatggcgc agccgccggc
     1921 gcatcggcgt gtccgaaccg gatcgcagac aaataccgta ggtctctagc cgcgtccggt
     1981 cgtggcgctc aaacaattcc accacctggt agctcacggc atggtcgtgg aaatccgccg
     2041 ataaatacgc cacgcgcaaa cgctcgctcg aaccggcggc cgcaagggta cccgagagcg
     2101 ccgatccgcc aaacttcttt gccgcgatgc ggtgcagttc gggatcgtcg aacgcgctca
     2161 aaagcggcca gggatagagc agctggccct ctcggcagcg gcgcaccaga tcgtccaccc
     2221 tggacgcgcg atcgcgccag tcgcacagca cgccggtggc gtaaaaggct tcgcacgcgg
     2281 cccgcccatt ggcggggtcc agcgccaagg ctttttccgc atccgccagc gcctcttcga
     2341 agcggccaag atcgcgccat agaccgacgc ggccgtagta cggcttggcg aaatttggat
     2401 cgagcgcgat ggcgcgatca tacgcggcca tcgcctccgc cgcccgctcc agcgccatga
     2461 gcgcgttgcc catgttgtga tagcccggcg atttgccagg agcgagcgcg acggaccgtt
     2521 cgcaatcggc cagggcttct ttatagcggc ccaattgaca gagcacgtcg ccgcgattgt
     2581 tgagcgcctc ggcgtaatcc ggcttcagcg caatcgcccg gccgaacgcg gccaacgcct
     2641 cctcgttgcg ctccaacgtc acgagcgtat tgccgcgatt gtagtacgct tcggcggagc
     2701 ccggactcag cataatggtc cggtccagcg cgttcagcgc ttcttccgtc cgatccagga
     2761 cgagaagcgc gttaccgcgg atgagatgcg tatcggcaat gttggggttt gcagcgatcg
     2821 cgcgctcgca ggcagccacg gcctgcgcgg cgtgtccgat atcgagcagg atgcgggcgc
     2881 gcgcgctatg cgcttcggcg aagtccggct tgagcgcgac ggcgcgctcg tagcttgcca
     2941 gcgccttggc cgcgcgtccc agcccggcat aagcgcctgc gatatggaaa tgtgccaatg
     3001 gcgagttggg gttgatctcc agcgccttgc cgatcaacgc cacggcgccg gtcatatccc
     3061 cgccctgggc gcgcagcacg cccaggaaat gcagagcgtc aaaattccgt ggctgggacg
     3121 acaatatcct gcgatagacg gcttccgcct cggccaggcg tccggcgtga tgcaggccga
     3181 gcgcttcgcg aagcgtgctg gtgagtgagt tcgcgcctgg catgatcctc ggacgatttc
     3241 aggccatcaa cctgtagccg ccgctttcag tcaccaggat gcgggcttcg ccggggttca
     3301 gctcgatctt ctggcgcaag cggtagatat gggtctccag cgtatgggtc gtgaccgcgg
     3361 gattgtagcc ccaaacctcg tgcagcagcg tctcgcgcgg caccgtgtcg ccgcagcgat
     3421 agaggtattt gaggatgttg gtttcctttt cggtcaggcg gattttcctg gcgtccgcgt
     3481 cgagcagcat tttggcgctg gggcggaacg tataggggcc gatgcggtac atcgcctcct
     3541 cgctgcggcc atggcttcga agatgcgcat gcacgcgcgc catcaggacc gcgaagcgga
     3601 acggcttggt gacgtaatcg ttggcgcccg atctaagacc cgcgatggta tcggcgtcgg
     3661 tgtcggcggc ggtcaggagc aggatcgggc aggtgacgcc ttggctgcgc agttcctggc
     3721 acagttcccg cccgtcgccg tcgggcaggc cgacgtccag gatcatgaat tcgtagagtc
     3781 cgtcgccggc cgccagccgg ccggcggcgc agtcctgcgc ttccaccacc gtgtaggtgc
     3841 cttcctggct caattgctcg gccaatgagg cgcgaagcat ggcatcgtct tccaccagaa
     3901 ggacgcgctt ggcgctggac atgggcggga atcatccaaa tttgcggtac cgagtcgagc
     3961 ctacccggtt tcagggcgct ggtgtattgg aagaacgcat gccgcaccgc ccacgctcaa
     4021 aacctcaagc tcccgccggc agccttcgcc gcgtggcgcg ggcggccgat gcgctgagag
     4081 cgggccggcc ggtgcgcctt acgggcggcg gcgcctttac ggtcctggcc gccgagaccc
     4141 tgga
//
LOCUS       DASWPU010000239         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_106168,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000239 DASWPU010000000
VERSION     DASWPU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106168"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VGC27_05190"
     CDS             complement(<1..836)
                     /locus_tag="VGC27_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281998.1"
                     /translation="MSGPSTKPVEKLSQTEATKELERLAREIFEHDRRYYREDAPTLL
                     DFEYDELRRRNAAIEARFPALVRADSPSHRVGARPSEKFGKVVHRVPMLSLDNAFAGE
                     DVTDFAARIRRFLGLKEEDDLVFTAEPKIDGLSASLRYENGVFVQGATRGDGAEGEDI
                     TANLRTLKDVPLRLAGQPPGVLEVRGEVYMTHADFAALNRRQEAEGEKLFANPRNAAA
                     GSLRQLDPSITAARPLHFFAYAWGEVSTLPEKTQWDMLQAFKSYGFQVNEEIRRCKKT
                     DEL"
     gene            complement(964..>2451)
                     /locus_tag="VGC27_05195"
     CDS             complement(964..>2451)
                     /locus_tag="VGC27_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0281999.1"
                     /translation="VFEPPAKHPVRGLLAGQSIPANGEIVLRRTLASDGRTRAFVNDE
                     PVGVALLKDIGASLLEVHGQADDRGLFDAATHRALLDASGGHEALAEEVAARFAALDA
                     AQTRIEALRRAHASASADADYVKQAVEELSALDAQEGEEASLAAERALLMNASRIVEE
                     VSAASELLSGERGAEASLALALKRLLRMNAEARQAASVAETALEQAYALAEEGRRELD
                     ALLSRLEVDSGELERKEERLFAIRAASRKYATQPDRLPLVLADFRARLDALDLGGGKL
                     KEAEAAVAASRAAYLDAGKRLSEARKSAARKLEAAVVGELQPLKLGHARFRVALTPLE
                     ESQAGASGLERVAFEVATLEGAPFGSLTKIASGGELARFSLAIKVALAQSGPPAALVF
                     DEVDRGVGGAVADAVGVRLQRLAQTTQVLLVTHSPQVAARAGRHFRISRKGDKLAVEL
                     LDDEARLEEVARMLSGAAVTGEARAAARRLLDEAQTLPKKARKKA"
BASE COUNT          381 a          846 c          816 g          408 t
ORIGIN      
        1 agctcgtccg tcttcttgca gcggcgaatt tcttcgttga cctgaaagcc gtaggatttg
       61 aacgcttgca gcatgtccca ttgggttttc tcgggaagcg tgctcacctc tccccaggca
      121 taggcgaaga aatgcagcgg ccgcgcggcg gtgatcgacg gatcgagctg gcgcagcgag
      181 cctgcggccg cattgcgcgg attggcgaag agcttctcgc cttcggcctc ctggcgccgg
      241 ttcagcgccg cgaaatcggc gtgggtcata tagacctcgc cgcgcacttc cagcacgccg
      301 ggcggctggc cggcgaggcg caacggaacg tccttgaggg tgcgcagatt ggcggtgatg
      361 tcttcgccct cggcgccgtc gccgcgcgtg gcgccctgca cgaagacacc gttctcataa
      421 cgcagcgagg cggacagccc gtcgatcttc ggttcggcgg tgaaaaccag atcgtcctcc
      481 tccttcagcc ccaggaagcg gcgtatgcgg gcggcgaaat cggtcacgtc ctcgccggca
      541 aaggcattgt cgagcgacag catcggcacg cgatggacga ccttgccgaa tttctccgac
      601 ggcctggccc cgacgcgatg cgacgggctg tcggcgcgga cgagggctgg aaagcgcgcc
      661 tcgatcgcgg cgttgcgccg gcgcaactcg tcatattcga aatcgagcag ggtgggcgca
      721 tcctcgcggt aatagcgccg gtcgtgctcg aagatttcgc gcgcgaggcg ttcgagttcc
      781 ttggtggctt cggtttgcga gagcttttcg acgggctttg tggaagggcc gctcacgcga
      841 accccccttc gtcgcccggc gctgcttcgc agcttcgggc gacacttccc ccgcgacccg
      901 tcttcgcggc taaagccgct tcgacgcggc aagcgcgggg gcagaaaagg accgggcaat
      961 ttctcatgct ttcttgcgcg ctttcttcgg cagggtttgc gcttcgtcga gcaggcggcg
     1021 ggcggcggca cgcgcttcgc cggttacggc ggcgcccgac agcatgcgcg cgacttcctc
     1081 cagccgcgcc tcgtcgtcga gcaactccac cgccagcttg tcgcccttgc ggctgatgcg
     1141 gaaatggcga ccggcgcggg cggccacctg cggcgaatgg gttacgagca gcacctgggt
     1201 ggtctgcgcc aagcgttgca ggcgcacgcc caccgcatcg gccaccgcgc cgccgacgcc
     1261 gcgatccact tcgtcgaaca ccagggctgc gggcgggccg ctttgcgcca gcgccacctt
     1321 gatcgcgagg gaaaaccgcg ccaactcgcc gccggatgcg atcttggtca gcgagccgaa
     1381 aggcgcgcct tccaaggtgg cgacctcgaa ggcgacgcgc tcaagccccg aggctccggc
     1441 ctgggattct tcgagcggcg taagcgcgac gcggaacctg gcatggccga gcttgagcgg
     1501 ctgaagttcg cccaccaccg ccgcttccag ctttcgcgcc gccgatttgc gcgcctccga
     1561 caagcgcttg cccgcgtcca gataggccgc gcgcgaagcg gccaccgccg cttcggcctc
     1621 tttcaacttg ccgccgccga gatcgagcgc atccaggcgc gcccggaaat cggccagcac
     1681 aagcggcaag cgatcgggct gggtggcgta tttgcgcgaa gcggcgcgaa tggcgaacag
     1741 ccgttcctcc ttgcgctcca actcaccgga atcgacttcc aagcgcgaca gcagcgcgtc
     1801 aagctcgcgg cggccctcct cggccaaggc gtaggcttgt tcgagcgcgg tctcggcgac
     1861 ggacgccgcc tggcgcgctt ccgcgttcat gcgcaacagc cgcttgaggg ccaaggccag
     1921 cgaagcttcg gcgccgcgtt cgcccgacaa cagttccgac gccgccgaca cctcttcgac
     1981 gatgcgcgaa gcgttcatca acagcgcccg ttcggcggcg agcgaggctt cctcgccctc
     2041 ttgggcgtcg agcgcggaca gttcctccac cgcctgtttg acgtaatcgg catcggcaga
     2101 cgcgctggcg tgcgcccggc gcaaggcttc gatccgggtc tgggccgcat ccaaggcggc
     2161 aaagcgcgcc gcgacctctt ccgccagcgc ttcgtgcccg ccggatgcat ccagcagggc
     2221 gcgatgggtg gcggcgtcga acaggccgcg atcgtcggcc tggccatgca cttccagcag
     2281 cgacgcgccg atgtctttca acaatgccac gccgaccggt tcgtcgttga cgaaggcgcg
     2341 ggtgcgccca tcggaggcca aagtccggcg cagcacgatc tcgccattcg ccgggatgga
     2401 ttgcccggcc agcaggccgc gtacgggatg cttcgccggc ggttcgaaca c
//
LOCUS       DASWPU010000240         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_118376,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000240 DASWPU010000000
VERSION     DASWPU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118376"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VGC27_05200"
     CDS             <1..601
                     /locus_tag="VGC27_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY0282000.1"
                     /translation="DLPVRRTADYEDVNLTVTSSSGFVLRRVFYSVPSQLIGHRLRGR
                     LFDDRLELFLGGTPLMTLARGRAHASGTNGHVVDYRHVIHSLRRKPMALLNLVYRDQL
                     FPRQAYARTFDALLAGLSPRDACKTMVALLGLAHERACEAELADYLAAGLDAGRLPDI
                     KALSLLFKRDGQAVPEVVITLTPLSAYDELGTVVIGEAA"
     gene            598..1407
                     /gene="istB"
                     /locus_tag="VGC27_05205"
     CDS             598..1407
                     /gene="istB"
                     /locus_tag="VGC27_05205"
                     /inference="COORDINATES: protein motif:HMM:NF006038.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY0282001.1"
                     /translation="MTAAEPIDTARLSLILNDLRLPAIKTLWPDFAARADKEGWPAAR
                     FLAALAEHEIAERARRRVERHIEEARLPPGKTLESFDFEAVPMVGKAQIMALAAGDSW
                     LQKGANIIMFGPPGGGKTHLAAALGLALVENGWRVLFTRTTDLVQKLQVARRNLELEA
                     ALAKLDKYHLLICDDLAYVTKDQAETSVLFELIGSRYERRSLLITANQPFGEWNKVFP
                     DQAMTLAAVDRLVHHATILEMNVESYRRREALERKRGPGRPANHATIKASS"
BASE COUNT          295 a          574 c          457 g          277 t
ORIGIN      
        1 ggatctgccg gtccgccgga cggcggatta cgaagacgtc aacctcaccg tgacctcgtc
       61 gagcggcttc gtcctgcgcc gggtcttcta ctcggtgccg tctcaactca tcggtcaccg
      121 gttacgcggc cgactgttcg acgaccgcct ggagctgttc ctcggcggta cgcccctgat
      181 gacgctcgcc cgtgggcgag cccatgccag cggcaccaat ggtcatgtcg tcgattaccg
      241 ccatgtcatc cattcgctcc ggcgcaaacc gatggcgctg ctcaatctgg tctatcgcga
      301 ccagctcttt ccccggcagg cttacgccag gaccttcgac gccctcctgg ccgggctctc
      361 gccccgtgac gcttgcaaga ccatggtcgc gctcctgggg ctggcccatg aacgagcctg
      421 cgaagccgaa ctggccgatt acctggccgc tggtctcgat gccgggcgtc tgcccgacat
      481 caaggcgctc agcctgttgt tcaaacggga cgggcaagcg gtccccgagg tcgtgatcac
      541 cctgacgccg ctcagcgcct acgacgaact cggtaccgtc gtcatcggag aagcagcatg
      601 acggccgccg aaccgatcga taccgcccgc ctcagcctga tcctcaacga tctcaggttg
      661 ccggccatca agaccctgtg gccggacttc gctgcccgcg ccgacaagga aggctggccg
      721 gcggcgcgct tcctcgccgc cctggccgaa catgagatcg ccgagcgggc tcggcgccgg
      781 gtcgagcgac acatcgaaga ggcccgcttg ccgccgggca agaccttgga gagcttcgac
      841 ttcgaggccg tgccgatggt cggcaaggcc cagatcatgg ccttggccgc cggcgactcc
      901 tggctgcaga aaggcgccaa tatcatcatg ttcggcccac ctggcggggg aaagacccat
      961 ctggcggccg ccctcggcct tgccctcgtc gagaatggat ggcgggtgct gttcacccgc
     1021 accaccgacc tcgtccagaa gctccaggtg gcgcggcgca acctcgagct cgaagccgcc
     1081 ctcgccaagc tcgacaaata tcatctgctg atctgcgacg atctcgccta tgtcaccaag
     1141 gatcaggccg agaccagcgt cctcttcgaa ctgatcggct cacgctacga gcggcgatcc
     1201 ctgctcatca ccgccaatca gccgttcggc gaatggaaca aggtcttccc cgaccaagcc
     1261 atgaccctgg ctgccgtcga ccgtctggtc caccacgcca ccatcctcga aatgaacgtc
     1321 gaaagttacc gccgccgcga agccctcgaa cgaaaacgcg gaccaggccg gcctgcaaac
     1381 cacgccacga tcaaagcgtc atcctgatcg tcgcgccgcg acaatcaacc ggcgaaaaat
     1441 aacgggttgt atctctgtcg cggaacgaca atcatcaccg cgtcgcgact gccaatcgcc
     1501 atcctgattg acgcgcgctt ctcatccaga ttgtcgcgcc ataggaaagc tgaacgctgg
     1561 cttctgcatg cctcgtggct accagccccc acaatccgtt ggg
//
LOCUS       DASWPU010000241         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_8156, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000241 DASWPU010000000
VERSION     DASWPU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8156"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VGC27_05210"
     CDS             <1..452
                     /locus_tag="VGC27_05210"
                     /inference="COORDINATES: protein motif:HMM:NF021587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HEY0282002.1"
                     /translation="RFFLINRYDAAHVDIGVVYDACAICGDAGYLQRGDEIICLACNV
                     RLFRPSIGKPGGCNPIPLPHTVRNGVIVIAASALEGGAHLFPEVVDIPVTDPVTGQAL
                     TNRIAPFQHEFEGHTYFFDSKDSYEKFRAAPQSYGRSRAPRAGGAEE"
     gene            473..1765
                     /locus_tag="VGC27_05215"
     CDS             473..1765
                     /locus_tag="VGC27_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0282003.1"
                     /translation="MLWRSLFEGRQRKILAASTVALAATLITTLFALSVDVGDKMAQE
                     MTAYGSNIRVTPKADAVPLLIGGVDYNPLKGRDYLDEQDLPKIKDIFWRNNIIGMAPR
                     LDLPAIMQAGGQTARATLIGTYFDHIVPLPDDESYRTGVRRINRFWQVEGKWPDEASD
                     RQVLAGHRLAARLNIGVGAVLSLRDAGKPGAPARAVTVSGLLTSGGEEDGALLAPLGL
                     VQGMAGLAGRVQSVDVSALTVPENDLSRKARRGSDALTSEEYDTWYCTAYVSSIAHQI
                     EEAVTDATARPIWQVAAGEGAVIDRIQMLLLVVSAAAIAAAAMCVSALMNTTVMERAR
                     EIGLMKALGAAPWEIHLLFIGEAAIVGLIGGAVGLAAGYGVAQAVGWSIFGSAVAVRP
                     ITIPVVLAISVITVLAGSILPARAIAALLPVEVLHGRR"
     gene            1752..>2285
                     /locus_tag="VGC27_05220"
     CDS             1752..>2285
                     /locus_tag="VGC27_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0282004.1"
                     /translation="MAAVEPRPYGRLRMFLLLIFRALTVRGSGVGTALGAILVGAAVV
                     SALTSLDFDISIKMSEELRAFGANMIVTPRSGHDAGRGSPGIDADTLRAAIATLPQDK
                     VVGASPFLYGVVHLDVGDAVLAGVDFAGLKAISPYWQVDGSWVTADFDDRNLMVGRHI
                     AQTMPLKVGSPVTIINRA"
BASE COUNT          363 a          740 c          761 g          421 t
ORIGIN      
        1 tacggttttt cctgatcaac cgctatgacg cggcccatgt cgatatcggc gtcgtttacg
       61 acgcctgcgc gatctgcggc gatgccggct atcttcagcg cggcgacgaa atcatctgcc
      121 tggcctgcaa tgtgcggctg ttccgcccgt cgatcggcaa gccgggcggc tgcaatccca
      181 ttcccctgcc ccataccgtc aggaacggcg tcatcgtgat cgccgcatcc gctcttgaag
      241 gcggggcgca tctttttccc gaggtggtgg atattccagt caccgatccg gtgacggggc
      301 aggccctgac caaccgcatc gcaccgttcc agcacgagtt cgagggtcac acttatttct
      361 tcgacagcaa ggactcgtac gaaaagttcc gcgccgcgcc gcaatcctat ggccgaagcc
      421 gggcaccgcg cgccggcggc gcagaggagt gaggaatgtc atggtgttga gaatgctgtg
      481 gcgctccctc ttcgaaggac gccagcgcaa gatactggcg gcgtccaccg tggccctggc
      541 cgccacgctg atcactacgc tgttcgcgct ttcggtcgat gtcggcgata agatggcgca
      601 ggaaatgacg gcctacggct ccaatatccg cgtcacgccg aaggccgatg ccgtgccgct
      661 gctgatcggc ggcgtcgact acaatccgct caaggggcgc gattatctcg acgagcagga
      721 tctgcccaag atcaaggata ttttctggcg caacaatatc atcggcatgg cgccgcgcct
      781 cgacctgccc gcgatcatgc aagcgggcgg ccagacggcg cgcgcgacgc tgatcggcac
      841 ctatttcgat cacatcgtgc ccctgccgga tgacgaaagc taccgcaccg gcgtgcgccg
      901 catcaaccgg ttctggcagg tcgagggcaa atggccggac gaagcttccg acaggcaggt
      961 gctggcgggg caccgcttgg ccgcacggct gaacatcggc gtgggcgcgg ttctatccct
     1021 gcgcgatgcc ggcaagccgg gcgcgcccgc gcgggcggtg accgtcagcg gccttctcac
     1081 cagcggagga gaagaggatg gcgcacttct cgccccgctc ggcctggtgc aggggatggc
     1141 gggccttgcc gggcgcgtgc agtcggtgga tgtcagtgca ctgacggttc ccgaaaacga
     1201 tctgtcgcgc aaggcgcggc gggggtccga cgccctgacc tccgaggaat acgacacctg
     1261 gtattgtacc gcctatgtca gttccatcgc ccaccagatc gaggaagccg tgaccgacgc
     1321 caccgcccgg ccgatctggc aggtggccgc cggcgaaggc gccgtcatcg accggattca
     1381 aatgctgctt ctggtggtga gcgccgccgc catcgccgcc gccgctatgt gcgtttcggc
     1441 gctgatgaac accacggtga tggaacgcgc cagagaaatc ggcctgatga aggcgctggg
     1501 cgccgcgccc tgggaaatcc acctgctgtt catcggcgag gcggcgatcg tcgggctgat
     1561 cggcggggcg gtcggcctcg cggcggggta tggcgtcgcc caggccgtgg gctggagcat
     1621 tttcggcagc gccgtggcgg tgaggccgat caccattccc gtggttctag ccatatcggt
     1681 gatcacggtg ctggcgggtt cgatcctgcc ggcgcgggcg atcgccgctc tgttgccggt
     1741 ggaggtgctt catggccgcc gttgagccgc gcccttacgg ccgtttgcgg atgttcctgt
     1801 tgctgatttt ccgcgccttg accgtgcgcg gcagcggcgt cggcaccgcg ctgggcgcca
     1861 ttctggtcgg cgccgccgtg gtctccgccc tgaccagcct tgacttcgac atttccatca
     1921 agatgagcga ggaactgcgg gcgttcggcg ccaatatgat cgtcacgccg cgttccggcc
     1981 atgacgccgg cagggggtcg cccggcatcg atgccgacac tttgcgcgcg gccatcgcca
     2041 ccttgccgca ggacaaggtg gtgggcgcca gcccctttct ctacggcgtg gtccatctcg
     2101 atgtcggaga tgccgtgctg gcgggagtgg actttgcggg cctcaaggcg atttcaccct
     2161 attggcaggt ggacgggtct tgggtgaccg ccgatttcga cgaccgcaac ctcatggtcg
     2221 gccgtcatat cgcccagacc atgccgctca aggtcggcag cccggtgacg atcatcaacc
     2281 gggcg
//
LOCUS       DASWPU010000242         4027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_87380,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000242 DASWPU010000000
VERSION     DASWPU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87380"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..808
                     /locus_tag="VGC27_05225"
     CDS             191..808
                     /locus_tag="VGC27_05225"
                     /inference="COORDINATES: protein motif:HMM:NF037152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0282005.1"
                     /translation="MAEKNTVRQQILDAAKKRFSHFGYAKTTMAEVAADCRMSPGNLY
                     RFFAGKLDIAEAIATVEHELHLEDMRKLLAAPGKSAGERLRDYFFEEMRRTYKTLEEN
                     PRIQEIAGLIASQRPHYADWMRASSRKLLVGLFDEAERNGEFRPGDKEFAVEMIQAAT
                     MKFRYPQLWTKLTLPQLERELDGVLKLVVNGLNAQKAHKHAAPAE"
     gene            957..1604
                     /locus_tag="VGC27_05230"
     CDS             957..1604
                     /locus_tag="VGC27_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY0282006.1"
                     /translation="MRRLANLVLIALLATGLGGAGQPAPKRLIEFSETGRADLDRVSA
                     YLNSIRTLRGDFVQIGPDGQIDQGRFYLEKPGRMRFEYRPPNPILIVADGRTVAVANS
                     KLKTVDRYPLSATPLSLILADKIDLRRNSAILAVRHEAGALIIEARTSTNRNRANLTL
                     VFADPELELRQWTIVDDQGLTTTVALRTLEQGVAVDARMFVLKDFKAPVGVKARD"
     gene            1607..2350
                     /locus_tag="VGC27_05235"
     CDS             1607..2350
                     /locus_tag="VGC27_05235"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY0282007.1"
                     /translation="MPSSRPSVGLVCDHRRVDGHPFHMAAEEYIAAVRDGAGALPLLI
                     PALDPPLASGDILASVDGLLFSGSQSNVSPCHYDSRPAREGTLLDERRDSTALPLLRA
                     AVEAGKPLLCICRGFQELNVALGGTLHQQVHEVPGRFDHREKGTSPDEQYAPAHPVEV
                     AQGGLLARILPRRGFEVNSLHGQGVDKLAPPLFAEAVAPDGQIEAVSLPGAKGFLLGT
                     QWHPEWRWAGNEISRAIFAAFGAALRGEA"
     gene            complement(2506..3315)
                     /locus_tag="VGC27_05240"
     CDS             complement(2506..3315)
                     /locus_tag="VGC27_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282008.1"
                     /translation="MAASALAVQPGIAASAWDGLWVLDDSASHYVDHSFTLTHGTGGI
                     WRYDDGSSNYSFRADGRPWPEPLAPGFSVVARVKGNMLDIVERGYGRDMERWHRILSP
                     DGNHLTGSDTRVYPDGRETTSPIFAVRVGSGNGFDGKWNQPADAPSDTQPSRPSSPLK
                     NAAMTSPPHFVISTADDGTMMWFLPATGELMRGRVDGRFRSLLGPQQPSRRTFAWRRL
                     GPGKLLFTCRDSGQVIETAIEELSPDGRTFTDTLWSAGHDNEKDVRVLAKR"
     gene            3414..3623
                     /locus_tag="VGC27_05245"
     CDS             3414..3623
                     /locus_tag="VGC27_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282009.1"
                     /translation="MVWLRAIRLSRNDPTLFSRHCGNESQVRIGDQFCWGGPSVREAF
                     AAPFSRSPDGEALRRIHDAPGLAET"
BASE COUNT          777 a         1290 c         1194 g          766 t
ORIGIN      
        1 agcgccgagt acccctatcc gctcataagg cgcagtcatg tcggatttct cttccatctc
       61 gggggttccg ctccggctgt aacacattgc ggaggattga acgatttgtt gaatattcag
      121 aaacagatac tatcctgaag ccgcgaagga aagcatgccg gccgcgggtg ccggcgcaag
      181 gggcaggctt atggccgaga agaatacggt tcgccagcag attctcgacg ccgccaagaa
      241 acgtttttcc cacttcggtt acgccaagac caccatggcc gaggtcgccg ccgattgccg
      301 aatgtcgccg ggcaatctct accgcttttt cgccggcaag ctcgacatcg ccgaagccat
      361 cgccaccgtc gagcacgaac tccatcttga ggacatgcgc aaattgctgg ccgcgcccgg
      421 caagagcgca ggcgaacggc tgcgcgacta tttcttcgag gaaatgcgtc ggacctacaa
      481 aacgctcgaa gagaatccgc ggattcagga aatcgccggc ctaatcgcca gccaacggcc
      541 gcactatgcc gactggatgc gcgccagctc gcgcaagctg ctggtggggc tgttcgacga
      601 ggccgagcgg aacggcgaat tccggcccgg cgacaaggag ttcgccgtcg agatgatcca
      661 ggccgcgacc atgaaattcc gttatccgca gctatggacc aaattgactc tgccccagct
      721 cgaacgcgaa ctcgacggcg tgctgaagct cgtggtcaac gggctcaatg cgcaaaaggc
      781 gcacaagcat gcggcgccgg cggaatagct ctatcccctt tcttccgccg caaatccgtc
      841 ctagagcatt tcaccgtttc acggaaacga tgaaatactc tagattctta gtttgtcgcg
      901 ttttcggacg gaaaaccggt ttccactttt cctgaaaacg ctttagagat ttcgcaatgc
      961 gccgcttggc aaaccttgtt ctgatcgcgc tcttggccac gggcctcggc ggcgccggcc
     1021 agcccgcgcc caagcgcctg atcgaattta gcgagaccgg ccgcgccgat ctcgatcgcg
     1081 tcagcgccta tctcaattcc atccgcacgt tgcggggcga tttcgtgcag atcggccccg
     1141 acggccagat agatcagggc cgtttctatc tggaaaagcc cgggcgcatg cgcttcgaat
     1201 accggccgcc caatccgatc ctgatcgttg ccgacgggcg caccgtcgcg gtggccaaca
     1261 gcaagctcaa gaccgtcgac cgctacccgc tgtcggcgac gccgctcagc ctgatcctcg
     1321 ccgacaaaat cgatctgcgt cgcaacagtg cgatcctcgc cgtccggcac gaggccgggg
     1381 cgctgatcat cgaagcgcgc accagcacca accgcaacag ggccaatctc actttggtgt
     1441 tcgccgaccc ggaactcgaa ctgcggcaat ggacgatcgt cgacgatcag ggactgacga
     1501 ccacggtggc gctgcgtacg ctggagcagg gcgtggccgt cgatgcgcgt atgttcgtgc
     1561 tcaaggactt caaggccccg gtcggagtca aagcgcggga ttgatcatgc cttcttcgcg
     1621 tcccagcgtc gggcttgtgt gcgatcaccg ccgggtggac ggacatccct tccatatggc
     1681 cgccgaggaa tatatcgccg ccgtgcgcga cggcgcgggc gccttgccgc tgctgattcc
     1741 ggcgctcgat cctccgctgg cctccgggga cattctggcg agcgtggacg gcttgttgtt
     1801 cagcggctcg caatccaacg tctcgccgtg ccattacgac agccggccgg cgcgcgaggg
     1861 aacgctgctg gacgaacgcc gcgactcgac cgcgctgccg ctgctgcgcg ctgcggtcga
     1921 agccggcaag ccgctgctgt gcatctgccg cggctttcag gaattgaacg tcgccttggg
     1981 cggcaccctg caccagcagg tccatgaagt gccgggccgc ttcgatcatc gcgaaaaggg
     2041 aacctcgccc gacgagcaat atgccccggc gcatccggtc gaggtggccc agggcggcct
     2101 gctggcgcgg attctgccgc ggcgcggttt cgaggtgaac tcgctgcacg gccagggcgt
     2161 cgacaaattg gcgccgccct tgttcgccga agccgtagcg cccgacggcc agatcgaagc
     2221 ggtgtcgctg ccgggcgcga agggctttct gctcggcacc caatggcatc cggaatggcg
     2281 ctgggccggc aacgagatct cgcgcgcgat cttcgcggct ttcggcgcgg cgttgcgtgg
     2341 ggaagcatga gccgagcgca cttctccatt ctgtcggtga atgtcggcct gcctgccgtc
     2401 atcggcacgg cgcacggcga agacgttctt tatttttgac gtcagccaga accacatgcc
     2461 tcgtcggttc cagccccctt gaaggctata cgccaaaaga aacggttacc gtttcgcgag
     2521 cacgcgcaca tctttctcat tgtcgtgtcc cgcgctccac aaggtgtccg tgaaggtgcg
     2581 accgtccggc gaaagctcct caatcgctgt ttcgatgacc tgcccgctgt cgcggcaggt
     2641 gaacaggagt ttgcctggac cgagccgacg ccacgcaaat gtccgccttg acggctgctg
     2701 cggaccgaga agggagcgga agcgtccatc cacgcgccct ctcatgagct cgccagtggc
     2761 tggcagaaac cacatcattg ttccgtcgtc ggcggtcgag atcacaaaat gcggcggtga
     2821 cgtcatggcc gcgttcttga ggggcgacga cggtctcgac ggttgcgtgt cggaaggcgc
     2881 atctgcaggc tgattccatt tcccatcgaa cccgttgcca gagcccacgc gcaccgcgaa
     2941 tataggcgat gtcgtctctc gaccgtccgg atacacgcgc gtgtcgctgc cggtaaggtg
     3001 attgccatcg ggcgaaagaa tgcgatgcca gcgttccatg tcccggccat atccgcgctc
     3061 cacgatgtcg agcatgttgc cttttacacg cgcgaccaca ctgaagcccg gagcaagcgg
     3121 ctccggccaa ggccggccat cggcacggaa actatagttt gaactgccat catcgtagcg
     3181 ccatatccca ccggtcccgt gagtcagcgt gaagctgtga tcaacataat gactggcact
     3241 atcgtcgagt acccacagac cgtcccaagc actcgccgcg atcccaggct gcactgccag
     3301 agcgctggca gccatcgcgg ctgcagttgc ggcagcggtc caaactctct tccgcatgac
     3361 accaccaaaa taacaacgct gttctcatat atcatgcttc tcgggcttcg cctgtggttt
     3421 ggctgcgggc catacggctc tcacgcaacg atccgacgct gttcagccgt cattgcggca
     3481 acgaaagcca agtccgcatc ggcgatcagt tttgctgggg cggtccaagc gtgcgtgaag
     3541 ccttcgccgc gccgttctcc cgctcacccg atggcgaggc gttgcgtcga attcatgatg
     3601 cgcccgggct cgcggaaacg tgacggcgcg ccatcaacgg aaaaatcgca gctttgccgg
     3661 ggctgattcg agacagtgtc aattttagca ctgtcattaa aaatatctta aggatgcggc
     3721 ggcacgagaa aatagttgaa acctgtgacc aaaatgcata taatcttctt acgaagggtg
     3781 attaagtccg gcaagcactg cccccccgct cgcaagctgg acaagccccc ttcgtcagcg
     3841 gtgtcggtca tcccctcccg gcacttcgcg cgtaacggcg caaacgacgc ccggctggca
     3901 cacccagccg ggcgtttttc tttggctctc aaagctgcca ggggctgcgg gtcggcacca
     3961 cgaagatgcg ttcgaaactg ccggcatggc cgtgtgtatt caaccattgc gcgaaccgtt
     4021 cgggttc
//
LOCUS       DASWPU010000243         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_63424,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000243 DASWPU010000000
VERSION     DASWPU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63424"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VGC27_05250"
     CDS             <1..226
                     /locus_tag="VGC27_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282010.1"
                     /translation="TGVLGFVLAGSRAQLAAGFLLLLLTLSLLLIIDIDRPTIGGIRE
                     SQSPMEQLRNSLKSQPPTVYDRWRTAPAAQ"
     gene            complement(259..1195)
                     /locus_tag="VGC27_05255"
     CDS             complement(join(259..858,860..1195))
                     /locus_tag="VGC27_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612137.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY0282011.1"
                     /translation="MSRAYSQDLRVRLVRYVEGGVSARAAAKVFGVSESAAIKWMQRW
                     RRQGSVAPDPVRGHRRQLLEGHADWLLQVIETKTDLTLEEIRSQLKKRGVRVSLWTVW
                     SFYDRHDISFKKSVYASEQDRADVTAARERWKAAQKLLDRTALVFIDETGTSTNMARV
                     RGRCRRGERLIGKLPHGHWKITTFVAGLRCDAITAPFVIDQPMNSAIFKAYLEQCLVP
                     TLKPGDIVVMDNLSSHKSDDVRQIIEAAGARLLYLPPYSPDLNPIELAFAKLKAHLRK
                     AAERSMPALWDRIGSILGTFSPVECANFFSHAGYA"
     gene            complement(1356..>1630)
                     /locus_tag="VGC27_05260"
     CDS             complement(1356..>1630)
                     /locus_tag="VGC27_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY0282012.1"
                     /translation="LLDEPTNDLDVETLRALELALEEFAGCAMIISHDRWFLDRMATH
                     MLAFEGDSHVEWFEGNFQDYEADKKRRLGIDADQPHRIKYKRFARA"
BASE COUNT          288 a          507 c          498 g          337 t
ORIGIN      
        1 cacgggcgtt ctgggctttg tgcttgccgg cagccgcgcg caactggccg ccggtttcct
       61 gctcctgttg ctgacgttgt cgctgctcct gatcatcgat atcgaccggc ccacgatcgg
      121 cggcattcgc gaatcccagt cgccgatgga gcagctgcgc aattccctga agagccagcc
      181 gcccacggtc tatgaccggt ggcgcactgc gcccgccgcg caatgagccc cagacattag
      241 agcgattttg gcttgggatt acgcatatcc tgcatggctg aagaagttcg cgcattcgac
      301 cggtgagaag gtaccgagga tcgagccgat acgatcccag agcgccggca tggagcgctc
      361 tgcggctttg cgcagatggg ccttcagctt ggcgaaggcc agttcgatcg ggttcagatc
      421 aggcgagtaa ggcggcaggt ataggagtct tgcgccagcc gcctcgatga tttggcgcac
      481 gtcgtcgctc ttgtgcgacg acagattgtc catcacgacg atgtcgcctg gcttcaaggt
      541 cggcaccaga cactgctcca gataggcctt gaagatcgcg ctgttcatcg gctggtcgat
      601 gacgaagggc gcggtgattg cgtcgcagcg caggccggcg acgaaggtgg tgatcttcca
      661 gtggccgtga ggcagcttgc cgatcagccg ctcgccgcgc cggcagcggc ctctgacgcg
      721 cgccatgttg gtgctggtgc cagtctcgtc gatgaagacc agggctgtgc gatcaagcag
      781 cttttgcgcg gctttccagc gttcccgcgc cgccgtcacg tcggcgcggt cctgttcgct
      841 ggcgtagacg cttttttttg aagcttatgt cgtgccggtc gtagaagctc cacacggtcc
      901 acaggctgac gcgcacgcca cgctttttca gctgtgagcg gatctcctcg agcgtcaggt
      961 cggtcttggt ctcaatgacc tgaagaagcc aatcggcatg gccctcaagc agttggcggc
     1021 ggtggccgcg aaccggatca ggggcgacgc ttccctgccg cctccagcgc tgcatccatt
     1081 tgatcgcggc actctcgctc acgccaaata ccttggccgc cgctcgcgcc gataccccac
     1141 cttcaacgta acgaaccaac ctaacccgca aatcctggga ataggctcgc gacatccacg
     1201 cctcccgaat cagcaacgag ccgagactcg cctatcagcc caactttggg aatccgctcc
     1261 gagtcagccc attccaaatt tgctctaggc ataacggaag ttgttgaaaa gtcgatctca
     1321 actctcaaca tgttgtgtct acgaatatcc gagccctagg cgcgggcgaa ccgcttgtac
     1381 ttgatcctgt gcggctggtc ggcgtcgatg ccgaggcggc gtttcttgtc ggcctcgtag
     1441 tcctggaagt tgccctcgaa ccactcgaca tggctgtcgc cctcgaacgc cagcatgtgc
     1501 gtcgccatgc ggtcgaggaa ccagcgatcg tggctgatga tcatggcgca gccggcgaat
     1561 tcctccagcg cgagttcgag cgcgcgcagg gtctcgacgt cgagatcgtt ggtcggttcg
     1621 tcgaggagga
//
LOCUS       DASWPU010000244         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_87404,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000244 DASWPU010000000
VERSION     DASWPU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87404"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1517)
                     /locus_tag="VGC27_05265"
     CDS             complement(153..1517)
                     /locus_tag="VGC27_05265"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY0282013.1"
                     /translation="MSVPQSAGGPIESRDDLVRYLAEGAKPKSEWRIGTEHEKFVYDL
                     KTRKPLPYEGTPGIHVLLEGMRRFGWEAVTEGGNVIGLSQNGASISLEPGGQFELSGA
                     PLRTVHQTCAEVNLHQQQVREVAAEIGAGAIGLGFAPTWTMDETHQMPKQRYAIMRRY
                     MPKVGGYGLEMMFRTCTVQVNLDFSSEADMVKKFRVGLALQPVATALFANSPFREGGH
                     NGFLSYRSHIWTDVDNRRSGMVPWVFEDGMGFERYVDYALDVPMYFVYRDGKYIDVAG
                     KSFRDFLARKIPEVKDIEPQLSDWADHLTTIFPEVRLKQFLEMRGADGGSWRRICGLP
                     ALWVGLLYDQASLDAAWDMVKDWTAEERQVLRDAVPRLAFKTPFRNTTVWELAHRMLE
                     ISTEGLRRRAAEDAAGMTEEGFLQPLGELVARGYTRAEELLQLYRTESGRSLDPLFGE
                     YNFL"
     gene            complement(1618..2385)
                     /locus_tag="VGC27_05270"
     CDS             complement(1618..2385)
                     /locus_tag="VGC27_05270"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY0282014.1"
                     /translation="MATDLTYSGGKVRLYVEPALGQGVRVVPDEGQAHYLLHVMRAKV
                     GDRVSLFNGRDGEWLARIAEASKRGCELECERLTAKQAGVPDLWLVFAPIKKTPADYL
                     TQKATELGVRALQPVITRRTIVTRVNLERMRANAVEATEQSGRLDVPDIREPLAFDKL
                     LAAWPRERRILFCDEAGATATAWGAPPIAEALRAAPECPWAVFTGPEGGFDPMERAAL
                     RALPFVAPVSLGLRILRADTAALAALSVWQAIKGDWR"
     gene            2591..>2824
                     /locus_tag="VGC27_05275"
     CDS             2591..>2824
                     /locus_tag="VGC27_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282015.1"
                     /translation="MTYNRSMNDGGSSKPADAVATSLVNRAPAKVQPYLRLMRLDRPI
                     GTWLLYWPCVFGLILGAVADSRPFGTWHDWGLVV"
BASE COUNT          534 a          927 c          866 g          497 t
ORIGIN      
        1 gcgggctcta tatcaaagtg tgcgccgaca aagcgcacaa cagcatcacc gtgaaataca
       61 aggacccgac cggcaaaggc ctgtccatcg acgacaaggc accttattgc ggcgcggcgg
      121 tgacggttca ctgacccgtc ggaccgtcaa gttcacagga aattatactc gccgaacagc
      181 ggatcgaggc tgcggcccga ttccgtgcgg tagagctgca gcagctcttc ggcgcgggtg
      241 tagccgcgcg cgaccagttc gcccagcggc tggagaaagc cttcttccgt catgcctgcg
      301 gcgtcttccg cggcgcggcg cctgagaccc tcggtggaaa tctcaagcat gcggtgggcc
      361 agttcccaca ccgtggtgtt gcggaacgga gtcttgaagg cgagccgcgg aaccgcatcg
      421 cgcaggactt ggcgttcttc cgccgtccag tccttgacca tatcccacgc cgcatcgagc
      481 gaagcctgat cgtagagaag accgacccag agcgccggaa gcccgcagat gcgacgccag
      541 ctcccgccgt cggcgccgcg catttcgaga aactgcttga gccgcacttc gggaaaaatc
      601 gtggtcaggt ggtccgccca gtcggacagt tgcggctcga tgtccttcac ttcgggaatc
      661 ttgcgcgcca ggaaatcgcg aaagctcttg ccggccacgt cgatgtactt cccgtcgcgg
      721 taaacgaagt acatcggcac gtcgagcgcg taatcgacat agcgctcgaa gcccatgccg
      781 tcctcgaaaa cccaaggcac catgccggag cggcgattat cgacgtcggt ccagatatgg
      841 ctgcgatacg acagaaagcc gttgtgcccg ccctcgcgga acggcgagtt ggcgaacagg
      901 gccgtcgcca ccggttgcag cgccaaaccg acgcggaact tcttgaccat gtcggcttcg
      961 gacgaaaaat cgagattcac ctggaccgtg caggtgcgga acatcatctc cagcccgtaa
     1021 ccgccgacct tgggcatgta gcgccgcatg atcgcgtagc gctgcttggg catctggtgg
     1081 gtctcgtcca tcgtccacgt cggcgcgaag cccagcccga tggcgcccgc gccgatctcg
     1141 gcggccactt cgcgcacctg ctgctgatgg agattcacct cggcgcaagt ctgatggacg
     1201 gttctgagcg gcgcaccgga cagctcgaac tgcccgcccg gctcaagact gatcgacgcg
     1261 ccgttctggc tgaggccgat aacgttgccg ccttccgtca ccgcctccca gccgaagcgg
     1321 cgcatgccct ccagcaagac gtggatgccg ggcgttccct catagggaag cggcttgcgg
     1381 gtcttcaaat cgtaaacgaa cttctcgtgt tcggtgccga tgcgccactc ggatttgggc
     1441 ttcgcaccct ccgccaggta gcgcacgagg tcgtcgcgcg attcgatggg gccaccggca
     1501 gactggggaa cggacatttc gtgccttttc cgggcgaacg gcccaaaacc gatatgcgaa
     1561 ttgcggcgcg cacgcgcgcc catccgttat gtagacgcgc actccgccgc cgacaagtca
     1621 gcgccaatcg cctttgatcg cctgccagac ggacagcgcc gccagcgcgg cggtgtcggc
     1681 gcgcaagatg cgcaagccca gcgaaacggg cgcgacgaaa ggcaaggcgc gcaatgccgc
     1741 gcgttccatc ggatcgaagc cgccttcggg gcctgtgaac acggcccaag gacattcggg
     1801 cgcggcgcgc aaagcctctg ctatcggcgg cgctccccaa gccgtcgctg tcgctccggc
     1861 ttcatcgcaa aagagaatgc gacgctcccg cggccacgcg gcgagcagct tatcgaatgc
     1921 gagaggttcg cggatgtcgg gcacgtcgag gcggcccgac tgctcggtgg cttccaccgc
     1981 gttggcgcgc atgcgttcga ggttcacgcg cgtcacgatg gtgcgccgcg tgatcaccgg
     2041 ttgcagcgcg cgcacgccga gttccgtcgc cttctgggtg agataatcgg ccggcgtttt
     2101 cttgatcggc gcgaaaacca gccagaggtc cggcaccccc gcctgcttcg cggtgaggcg
     2161 ttcgcattcc agctcgcagc cgcgcttgga cgcttccgcg attcgcgcca gccattcgcc
     2221 gtcgcgtccg ttgaacaggc tgacacgatc accaaccttg gcgcgcatga cgtggagaag
     2281 gtaatgggcc tggccttcgt ccggcacgac gcgcacgccc tgccccaacg ccggctcgac
     2341 gtagaggcgc accttgccac cggaataggt gaggtctgtt gccatttctc gacactcgca
     2401 cagggttttg cagtagaata caacgtgtag cactcatcat aagccgacat gaacaaacct
     2461 cccagaagcc gccaaattcc gaagggccaa gccgttccgc gccgcaccga agcatttgat
     2521 cgagtattcc ttaggcttgc cgaagttact ttcgaggaat ggaataaacc gcaagacgaa
     2581 atcgcattcc gtgacctata accgaagcat gaacgatggt ggctcgtcca aacccgccga
     2641 tgccgtggcc accagcctgg tgaaccgcgc cccggccaag gtacagccct atctcaggtt
     2701 gatgcggctg gaccggccca tcggcacctg gctgctttat tggccttgcg tgttcgggtt
     2761 gatcctcggc gccgtggccg acagccgccc cttcggcact tggcacgatt ggggacttgt
     2821 ggtt
//
LOCUS       DASWPU010000245         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_1885, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000245 DASWPU010000000
VERSION     DASWPU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VGC27_05280"
     CDS             <1..1111
                     /locus_tag="VGC27_05280"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEY0282016.1"
                     /translation="LLKVPVARDGLFALNGVVTALTIATKAGTSFVFGMVGGGDPPFA
                     VTNPSGMVNFAFGILPLVIVISALSALLWHWKILPIIVKAVAFVLRKLLGVGGAVGLG
                     GGSMVFLGNVEGLLVIRPYLSRLTRAEMFILFTVGMAVCSGTVFVLYAMILKDVLPGA
                     LGHVLVASFLSLVSAIVIGRIIMPSEAGIDATDQGSRYRSSMDSIAHGTEEGLKLYWQ
                     IIAMLVVVVALVALLNVILASFPPVWGSPLTLQRVFGWLFAPVVWLIGIPWEQAATAG
                     SLLGLKTSLNEVIAYLGMAALPQGALDPRSIMIMVYALCGFANFSSVGILIAGTSALV
                     PERRDEIVPLALRALVSGTMASCLTGAMVGFLPVA"
     gene            1286..1852
                     /locus_tag="VGC27_05285"
     CDS             1286..1852
                     /locus_tag="VGC27_05285"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0282017.1"
                     /translation="MSLKNIPQAAASDSGRPPEKLSHRIAVSEIDSSILLQGAAYWRG
                     LCRERKFPSRSAVAPRGLAGLLRYTTLLRVIDGGEDYEYRIVGDAYVMAHGTSFQGKR
                     WSDTERVAPGYYKTVKSTYDSVVRTGEPVAMRGWIERGGKNSELVYSEYLFLPLGEND
                     QTVDHILVFAVYLPNENAASVRKAGVSS"
     gene            1882..>2110
                     /locus_tag="VGC27_05290"
     CDS             1882..>2110
                     /locus_tag="VGC27_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282018.1"
                     /translation="MARDAATGSHDTRKAERRLAGLLREIRACRICAAHLPHGVNPVI
                     HAGATARLCIVAQAPGIRVHETGISFNDPSGD"
BASE COUNT          335 a          660 c          676 g          439 t
ORIGIN      
        1 gcttttgaag gtgccggtgg cgcgcgacgg tctgttcgcg ctcaacggcg tcgttaccgc
       61 gctgacgata gcgaccaagg ccggcacctc cttcgtcttc ggcatggtcg gcggcggcga
      121 tccgccgttc gccgtcacca atcccagcgg catggtgaac ttcgccttcg gcattctgcc
      181 tttggtgatc gtgatctcgg cgctgtcggc gctgctgtgg cactggaaaa tccttcccat
      241 catcgtcaag gccgttgctt tcgtcctgcg caagctcctg ggggtcggcg gcgcggtcgg
      301 gctgggcggc ggctcgatgg tctttctcgg caatgtcgag ggcctgctgg tgatccgtcc
      361 ctacctctcg cgcctcaccc gcgcggagat gttcatcctg ttcaccgtgg gaatggcggt
      421 ctgttctggc accgtcttcg tgctctatgc gatgatcctg aaggatgtgc ttcccggcgc
      481 gctcggccat gtcctcgtcg cgtcgttctt gtcgctggtg agcgccatcg tgatcggccg
      541 gatcatcatg ccgagcgagg ccggcatcga cgcgaccgat caaggttcgc gctaccgctc
      601 cagcatggac tcgatcgccc atggaacgga ggagggcctc aagctctatt ggcagatcat
      661 cgcgatgctg gtcgtcgtcg tggcgctggt ggcgctgctc aacgtgatcc tcgccagctt
      721 cccgccggtg tggggatcgc ccctgacctt gcagcgcgtg ttcggctggc tgttcgcgcc
      781 ggtggtgtgg ctgatcggga taccgtggga gcaagcggcg accgccggca gcctgctcgg
      841 cctcaagacc agcctcaacg aagtgatcgc ctatctcggc atggcggcgc tgcctcaagg
      901 cgcgctcgat ccgcgctcga taatgatcat ggtctatgcc ttgtgcggct tcgcgaactt
      961 ctcctcggtc ggcatcctga tcgcgggcac cagcgcgctg gtgccggagc ggcgcgacga
     1021 gatcgttccg ctggcattgc gcgcgctggt atcgggaacg atggcaagct gtctgaccgg
     1081 cgcgatggta ggctttctgc cggtcgcttg accgggcgcc gatctcgcat cgctggcagt
     1141 gctgctcgcc caagttgcaa tcaccgatta gtcgcgtagc gttttgtgat tctggcataa
     1201 gggcgcaggc gtcattttcc taaacgttcc tttaaccacc cataggcgac gctagggaaa
     1261 gatacccgat tagcaaatcc tgcaaatgtc cctgaaaaat atcccgcaag ccgcagcgtc
     1321 ggactctggc cggccgccgg agaaattgag ccatcgcatc gcggtatccg aaatcgacag
     1381 cagcatcttg ctgcagggcg ccgcttattg gcgcggtctg tgccgcgagc ggaaattccc
     1441 ctcgcgttcg gccgtcgcgc cgcgcggtct ggccggtctg ctgcgctaca ccacgctgct
     1501 gcgtgtgatc gacggcggcg aggattacga atatcgcatc gtcggcgacg cctatgtgat
     1561 ggcgcacggc acctcgttcc agggaaagcg ctggagcgac accgaaaggg ttgctcccgg
     1621 ctattacaag acggtcaagt ccacttacga cagtgtggtg cgcacaggcg agccggtcgc
     1681 catgcgcggc tggatcgaac gcggcggcaa gaattcggag cttgtctaca gcgaatatct
     1741 ttttctccct ttaggggaaa atgatcagac tgtggatcac atcctggtgt tcgccgttta
     1801 ccttccgaac gagaacgccg cgagcgtgcg caaggcgggc gtttcaagct agacttccct
     1861 cctgtcttgt cgggaggaag gatggcgcgc gacgccgcaa ccggttcaca cgacaccagg
     1921 aaggccgagc gcaggctggc cggattgctg cgcgaaatcc gcgcctgccg gatttgcgcg
     1981 gcgcatcttc cgcacggcgt gaacccggtg atccatgccg gcgccacggc aaggctctgc
     2041 atcgtggcgc aggcgccggg catccgcgtc cacgagaccg ggatttcctt caacgatcct
     2101 tcgggcgacc
//
LOCUS       DASWPU010000246         5598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_112415,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000246 DASWPU010000000
VERSION     DASWPU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112415"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..538
                     /gene="ccmD"
                     /locus_tag="VGC27_05295"
     CDS             377..538
                     /gene="ccmD"
                     /locus_tag="VGC27_05295"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEY0282019.1"
                     /translation="MSNFLSQGGYGFYVWSAYAVSVLGIGLLIASTLAAWRKAKRALA
                     ALQDSETNF"
     gene            551..769
                     /locus_tag="VGC27_05300"
     CDS             551..769
                     /locus_tag="VGC27_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282020.1"
                     /translation="MQISAANLLIASQQGAKAAPRPDGAFAAALVKEKPGFEPLNFKQ
                     AAAPKLAAAAPPSPQGVAPLGSKIDIRV"
     gene            complement(784..1290)
                     /locus_tag="VGC27_05305"
     CDS             complement(784..1290)
                     /locus_tag="VGC27_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY0282021.1"
                     /translation="MKGQRRPNSGFELAEAPSHLIRRCEQYFGDLYTREAGNCELTKQ
                     QFTVLAALENNEGVSQTALVEMTGIDRSTLAEMVRRMLKRGLVSRERTETDARANAVA
                     ITGNGRKELRAARSAANRADRLLLDAVPPSERGKFLKNLSAVAAAGEVFAANGSGKAR
                     RKTRRRRN"
     gene            complement(1572..2120)
                     /locus_tag="VGC27_05310"
     CDS             complement(1572..2120)
                     /locus_tag="VGC27_05310"
                     /inference="COORDINATES: protein motif:HMM:NF001323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEY0282022.1"
                     /translation="MRRTILDFGPLLLFFAAYKLFDLTVATATVMAAASIAVAIGYWF
                     DRKIHPVPLVTAVIVLVFGGLTLYLNDDTFIKMKPTIIYALFGAALLGGLYFNRHFVK
                     FILGAAMTLNEAAWRVLTWRFGLFFFAMAILNELIWRNFSSDVWVDYHVFGAIALTVL
                     FSVSQVPFLLKHQIETESSSAD"
     gene            complement(2129..3067)
                     /gene="ftsY"
                     /locus_tag="VGC27_05315"
     CDS             complement(2129..3067)
                     /gene="ftsY"
                     /locus_tag="VGC27_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY0282023.1"
                     /translation="MRQFGEAPPPPTFFERLKQGLAKSTGALGLGEILLKKKLDADMV
                     AALEEALIRADLGAKAAHEIAGAVAKSRYDEDISPAELRKVLATEIARVLRPVEMPLR
                     IDRTKQPFVVLVAGVNGTGKTTTIGKIAAKLVRDGHKVVLAAGDTFRAAAIEQLQIWG
                     KRAGAEVFSRAPGADAAGLVFDALARARATGADVLLVDTAGRLQNKAGLMAELEKIAR
                     VLKKQDAAAPHAVLLVLDATTGQNAVAQVEAFAACVPLTGLVMTKLDGSAKGGILVAL
                     AAKFALPVHFIGVGEKEDDLQPFAAEAFAKALTGAA"
     gene            complement(3064..4308)
                     /gene="mtaB"
                     /locus_tag="VGC27_05320"
     CDS             complement(3064..4308)
                     /gene="mtaB"
                     /locus_tag="VGC27_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEY0282024.1"
                     /translation="MSADIVTFGCRLNAYESEAIRARAAEAGLSDTVIVNTCAVTAEA
                     VRQSRQAIRKLRRERPGARIIVTGCAAQTEPDTYAGMQEVDLVLGNREKLEAESYAWF
                     AATERVRVNDIMSVKETASQFVESFDGKSRAFLQVQNGCDHRCTFCIIPYGRGNSRSV
                     GAGAVVEEARRLTENGFGEIVLTGVDLTSYGADLPGTPTLGALVRKILKLVPDLKRLR
                     LSSIDSIEADAELMSAIAEEERLMPHFHLSAQSGDDMILKRMKRRHARAHTVRFCDEV
                     RRLRPEAAFGADLIAGFPTETEEMFENTLKLVDDAGFSTLHVFPFSARKGTPAARMPG
                     VPRAVAKERAGRLRAKGAAALAKRLAGLVGSEQMLLMENAGLGRTPCFTPVRVAGGAA
                     PGEFRRVRIADAAQDHLVAAAP"
     gene            complement(4305..5129)
                     /gene="dapF"
                     /locus_tag="VGC27_05325"
     CDS             complement(4305..5129)
                     /gene="dapF"
                     /locus_tag="VGC27_05325"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009565969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY0282025.1"
                     /translation="MIPFLKMHGLGNDFAVFDARKQGLVLDAASARAIADRRRGIGCD
                     QVIVIAASDGADAAMRIFNADGGEVESCGNAARCVARLLMAELDRDTVRLNTPGGPLV
                     CCDAGGGLVTVDMGAPVLAWDKVPLSCPADTSRFMLSVDGASHPVSAVSMGNPHCVLI
                     VDDAEAAPVASLGPRIETHPMFPKRTNVEFVSVRDRSHLRMRVWERGAGITQACGTGA
                     CAAAVAAHRRGLADAKVEVELDGGVLTIELRDSHVFMTGPAALSFRGEIDLAELTA"
     gene            5229..>5598
                     /locus_tag="VGC27_05330"
     CDS             5229..>5598
                     /locus_tag="VGC27_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282026.1"
                     /translation="MRRFAIMLIGLVAATAALAADKGKTVEDPYIWLEEVHGAGPLAW
                     VAEQNKKSLGVLKSDPRYRTNYDSILEVLDATDRIPYGWLDHQYVFNFWQDAEHPKGI
                     WRRTTIADYANASPNWDVLLD"
BASE COUNT          985 a         1839 c         1755 g         1019 t
ORIGIN      
        1 cggcgggcgc gcacgctgat gctggcgagg gctagagcat gatctcctgg gatggaagcg
       61 cctgcgccga ggggattttg cggcgtgggc aggagaaggg cgaagcgcgt agcgggtact
      121 acgggcgagc ccttcgacga acccacgacg caaaagcccc cggccctgcg ggttgtgtgc
      181 cggcgggcgt ccgctttgtt gcggcgctcg gccgatgtcc ccgcatcgcc cttcgcgccg
      241 cgcctggcgg cctacgctgc ttcgcagctc cggtccgccc tgtgccctgc ttgcccgccg
      301 gagctttagc gaaggcgggc ggattgcctc gccggcacgc aacgcagacg gttctatccc
      361 aggagatcat gctccgatga gcaattttct gtcgcagggc ggctatggtt tctatgtgtg
      421 gtcggcttac gcggtcagtg tccttggcat cggcctgctg atcgcgtcca cgctcgccgc
      481 ttggcgcaag gcgaagcgcg cacttgccgc tcttcaggat tcggaaacca atttttgaaa
      541 ctctgcggcc atgcagatca gtgccgccaa tcttctgatc gcctcgcaac agggagcgaa
      601 agccgcacca cgtcccgacg gcgcattcgc cgcggctttg gtgaaggaaa agccgggctt
      661 cgagccgctg aacttcaaac aggcggcggc gcccaagctt gccgcggccg cgccgccttc
      721 cccccagggg gttgcaccct tgggctcaaa gatcgatatt cgcgtttagt ttcagccgat
      781 tgctcagttg cggcggcgac gggttttgcg ccttgctttt cccgagccgt tggccgcgaa
      841 aacctctccg gcggcggcga ccgccgacag gttcttgagg aatttgccgc gctcggacgg
      901 cggtacggca tcgagcagca gccggtcggc ccggttggcc gcggaacgcg ccgcgcgcaa
      961 ctccttgcgg ccattgccgg tgatggcgac ggcgttggcg cgggcatccg tttcggtgcg
     1021 ctcgcgcgac accagaccgc gcttcagcat gcggcgcacc atctcggcca gggtcgaacg
     1081 gtcgatgccg gtcatctcga ccagcgcggt ctggctgaca ccctcattgt tctcaagcgc
     1141 ggcaagcacg gtgaactgct gcttggtcag ttcgcagttg ccggcttcgc gcgtgtaaag
     1201 atcgccgaaa tattgttcgc accggcgaat aagatgcgac ggcgcttctg caagttcgaa
     1261 gccgctgttc ggcctgcgct gacccttcat gagacgcccc tgcccgttga aatgcattcg
     1321 accggcgtta cgtgccaatc cgaattcgaa aaaaccatcc ccagaacgcc gggagctcca
     1381 cgcgtttgcc aattggcgcg catacggaac aatagacaag catacgtccg acgacaactc
     1441 tctctatcgc gttttatacg gatttaggca atgcgtaaaa tactaacgcc gtctttcgac
     1501 ccgccggcgt cttcgcgctt tgcgcgcagc actgctatct gtgacggcga ttccggcgga
     1561 tacaacaagc tctagtcggc ggacgaagat tctgtctcga tctgatgctt gagcaggaac
     1621 ggcacctggc ttacggagaa caaaacggtc agcgcgatgg cgccgaagac gtggtagtcg
     1681 acccacacgt cgctggaaaa attgcgccag atcaattcgt tgaggatcgc catcgcgaaa
     1741 aagaacagac cgaagcgcca ggtgagaacg cgccaggcgg cctcgttcag cgtcatcgcc
     1801 gcgcccaaaa tgaatttgac gaaatgccgg ttgaaataaa gtccgccgag cagcgccgct
     1861 ccgaacagcg catagattat ggtcggcttc attttgatga aagtgtcgtc gttcagatag
     1921 agcgtcagtc cgccgaacac caggacgatg acggcggtca caagcggcac cggatggatt
     1981 ttgcggtcga accaatagcc gatagctacg gctatgctgg ccgcggccat gacggtcgcg
     2041 gttgcaacgg tgagatcgaa caatttgtac gccgcgaaga acagcagcaa aggcccgaaa
     2101 tcgaggatag tgcgtctcaa ttgaggattc atgcggcccc cgtcagcgct ttggcgaacg
     2161 cttcggccgc gaagggctga agatcgtcct ctttttctcc cacgccgatg aaatgaacgg
     2221 ggagggcgaa tttggcggcc aatgctacca gaatgccgcc tttggcgctg ccgtccagtt
     2281 tggtcatcac cagcccggtc aagggaacgc aggccgcgaa cgcttccacc tgtgcaaccg
     2341 cgttctgtcc cgtggttgca tccagcacga gcagcacggc gtgaggcgcc gccgcgtcct
     2401 gcttcttaag aacgcgggcg attttctcca attccgccat gagaccggct ttgttctgca
     2461 agcggccggc cgtatcgacg agcagaacgt ccgctcccgt cgcccgcgcc cgcgcgagcg
     2521 cgtcgaagac aagccccgcc gcgtcggcgc cgggcgcgcg cgaaaacact tcggcgcccg
     2581 cgcgcttgcc ccagatctgg agctgctcga tggccgccgc acggaaggtg tcccccgcgg
     2641 ccagcacaac tttatgtccg tcgcgcacca gcttggccgc aatcttgccg atggtggtgg
     2701 ttttgcccgt gccgttgacg ccggccacca acacgacgaa tggttgtttc gtgcggtcga
     2761 tgcgtaaagg catctcgacc ggccgcaaca cccgcgcaat ctcagtcgca agaaccttgc
     2821 gcagttccgc cggcgaaata tcctcgtcgt agcgggattt ggcgacggct ccggcgatct
     2881 cgtgcgccgc cttggcgccc agatcggcgc ggatcagcgc ttcctcgagc gccgcgacca
     2941 tatcggcgtc gagttttttc ttgagcagaa tttcgccaag gccgagcgcg ccggtgcttt
     3001 tcgccaggcc ttgtttcagc cgctcgaaga aggtcggcgg cgggggcgct tcgccgaact
     3061 gcctcatggc gcggccgcca ccagatgatc ttgcgccgca tcggcaatgc gcacgcgcct
     3121 gaattcgccc ggcgcggcgc cgcccgcgac gcgcacgggt gtaaagcacg gcgtacggcc
     3181 gagaccggca ttttccatga gcaacatttg ttcgctgccg accagtccgg caagccgctt
     3241 ggcgagcgcc gctgcgccct tggcgcgcag ccgtccggcg cgctccttgg cgacggcacg
     3301 cggcaccccg ggcatgcgcg cggccggcgt gcccttgcgg gcgctgaagg gaaagacgtg
     3361 cagcgtcgag aacccggcat cgtccaccag cttcagcgtg ttctcgaaca tctcttcggt
     3421 ttccgtcggg aacccggcga tcaaatccgc gccgaaggcg gcttcgggcc gcagccggcg
     3481 gacctcgtcg cagaatcgaa cggtgtgagc gcgggcatgg cggcgcttca tgcgtttcaa
     3541 aatcatgtcg tcgcccgatt gggccgagag gtggaaatgc ggcatcagcc gctcttcctc
     3601 ggcgatggcg ctcatcagtt ccgcatcggc ctcgatggaa tcgatggacg agagccggag
     3661 ccgcttcaaa tccggaacca gcttcaggat cttgcgcacc agcgcgccca gcgtcggcgt
     3721 gcccggcagg tccgcgccgt aggaggtgag gtccacgccg gtcagcacga tttcgccgaa
     3781 gccgttttcc gtcaggcggc gcgcttcctc gaccaccgcg cccgccccca cgctgcgcga
     3841 attgccgcgc ccgtaaggga tgatgcagaa ggtgcagcga tggtcgcagc cgttctggac
     3901 ttgcaggaag gcgcggctct tgccgtcgaa gctttccacg aactgcgacg ccgtctcctt
     3961 cacgctcatg atgtcattga cgcgcacccg ctccgtcgcg gcgaaccagg catagctttc
     4021 cgcttccagc ttttcgcggt tgccgagcac cagatcgact tcctgcatcc cggcataggt
     4081 gtcaggttcg gtctgcgccg cgcagccggt gacgatgatg cgcgcgccgg gccgctcgcg
     4141 ccgcagcttg cgaatggcct ggcgcgactg gcgcacggct tccgccgtca ccgcgcaggt
     4201 gttgacgatc accgtgtcgc tgaggcctgc ttcagcggcg cgcgcgcgga tcgcttcgga
     4261 ttcgtaggcg ttcaagcggc agccgaaggt gacgatatcc gcgctcacgc cgtcagctcc
     4321 gcgaggtcga tttcaccccg gaaggacagc gccgccggtc ccgtcatgaa gacatggcta
     4381 tcgcgcagtt cgatggtcag cacgccgccg tcgagttcga cttcgacctt ggcgtctgcg
     4441 aggccgcgcc ggtgcgccgc caccgccgcc gcgcaagcgc ccgtgccgca ggcctgggtg
     4501 atgcctgcgc cgcgctccca cacgcgcatg cgtagatggg agcggtcgcg tacgctgacg
     4561 aattctacat tggtgcgctt ggggaacatg ggatgggtct cgatgcgcgg accgagactt
     4621 gccaccggcg ccgcctcggc atcgtccacg atcagcacgc aatggggatt gcccatcgaa
     4681 acggccgaga ccgggtgaga ggctccgtcc acgctcaaca tgaagcggct ggtatcggcg
     4741 gggcaggaca acggcacctt gtcccacgcc agaaccggcg cgcccatgtc caccgtgacc
     4801 agcccgccgc ccgcgtcgca gcagacgaga gggccgcccg gcgtattgag gcgcaccgta
     4861 tcgcggtcca attccgccat caacaggcgc gccacgcagc gcgcggcatt gccgcaagat
     4921 tcgacttcgc cgccgtcggc attgaaaatg cgcatcgcgg catccgcccc gtccgaggct
     4981 gcgatcacaa ttacctgatc acagccgatg ccccgccgcc ggtcggcgat ggcgcgcgcg
     5041 ctggcggcgt cgagcacaag cccttgcttt cgcgcgtcga ataccgcgaa atcgttgccc
     5101 aggccgtgca ttttgaggaa cgggatcatt acgggcctat ataggccgtg acttcttcgc
     5161 ccaccagtgc cggattgacc tttccgcccc gctttcgttc aaacttcgca ctttcaaagg
     5221 aggatcggat gcgccgtttc gccataatgt tgatcgggct cgttgccgcc accgctgcgc
     5281 tcgcggccga caagggaaag accgtggaag acccctatat ctggctggaa gaggttcacg
     5341 gcgcggggcc gctcgcctgg gtggcggagc agaacaagaa gtcgctcggc gtgctcaaat
     5401 ccgatccgcg ctaccggacg aattacgatt cgatcctcga ggtgctggat gccaccgacc
     5461 gcattcccta tggctggctc gaccatcaat atgtcttcaa tttctggcaa gacgccgagc
     5521 accccaaggg catctggcgg cgcaccacca tcgccgatta cgccaacgca agccccaact
     5581 gggatgtcct gctcgatc
//
LOCUS       DASWPU010000247         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_75417,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000247 DASWPU010000000
VERSION     DASWPU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75417"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1039)
                     /locus_tag="VGC27_05335"
     CDS             complement(2..1039)
                     /locus_tag="VGC27_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY0282027.1"
                     /translation="MKALAYLGPGKKALEERPTPKIVESTDAIVKITKTTICGTDLHI
                     LKGDVPSCQPGRILGHEGVGIVDKAGPDVTVFKVGDRVLISCISACGKCFYCRKLMYS
                     HCTTGGWILGNRIDGTQAEFVRIPYADTSLYRIPDGADEEALVMLSDILPTGFECGAL
                     NGKVQPGATVAIVGAGPVGLAALLTAQFYSPAEVIMVDLDDNRLEIAKRFGATAAINS
                     ADGKAVETIMKMTGNRGVDTAIEAVGIPATFELCAEIVAPGGTIANAGVHGVKVDLHL
                     EKLWDRNITITTRLVDTVSTPMLLNVLGAHKIDAKLLITHRFKLDRILDAYETFAHAA
                     DTRALKVIIEA"
     gene            complement(1121..>1639)
                     /locus_tag="VGC27_05340"
     CDS             complement(1121..>1639)
                     /locus_tag="VGC27_05340"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEY0282028.1"
                     /translation="PGNRSAGAAATGRTQTNPNAARPQGATPGNRSAGGAVTGATRAN
                     TNAVNSRGARTQNRSPAGVTTNSRGNSMSGNNAGRQPSINSLRRNVQAPRRFHNGSYR
                     APQGYQARRWSHGNRLPRNYFVRDYWITNFMMFGLFAPPPNLVWVRVGNDALLVDRYS
                     GDIVQVDYGVFY"
BASE COUNT          290 a          482 c          546 g          321 t
ORIGIN      
        1 cttaagcctc gatgatcacc ttgagcgccc gcgtgtcggc ggcgtgcgcg aaggtttcgt
       61 aggcgtccaa gatgcgatcg agcttgaagc gatgggtgat caggagcttg gcgtcgatct
      121 tgtgggcgcc caggacgttg agcagcatcg gcgtgctgac ggtatcgacc aggcgcgtgg
      181 tgatcgtgat gttgcggtcc cacagcttct ccaggtgaag atcgaccttg acgccgtgca
      241 cgccggcatt ggcgatcgtc ccgcccgggg cgacgatttc cgcgcataac tcgaaagtgg
      301 ccggaatgcc caccgcctcg atggcggtgt cgacgccgcg attgccggtc atcttcatga
      361 tcgtctccac ggccttgccg tcggcgctgt tgatggccgc cgtggcgccg aagcgcttgg
      421 cgatttccag gcggttgtcg tcgagatcga ccatgatgac ctcggccggc gaatagaact
      481 gagcggtgag cagcgcggcg agtccgacag gacctgcgcc gacgatcgcc acggtggcgc
      541 ccggctgaac cttgccgttg agtgcgccgc actcgaatcc ggtcggcagg atgtcgctca
      601 gcatcaccag cgcttcttcg tccgcgccgt cgggaatgcg atagaggctg gtgtccgcat
      661 agggaatgcg gacgaactcg gcttgcgtgc cgtcgatgcg gttgccgaga atccaaccgc
      721 cggtcgtgca gtgcgagtac atgagttttc ggcaatagaa gcacttcccg caggcgctga
      781 tgcaggaaat gagaacccgg tcgcccacct tgaacaccgt gacgtcaggg ccggccttgt
      841 cgacgatgcc gacaccttca tgacccagga tgcggccggg ctgacagctg gggacgtcac
      901 ctttgaggat gtgcaagtcc gtgccgcaaa tggtcgtttt cgtgatcttg acgattgcgt
      961 ccgtggactc cacgattttc ggggtcgggc gctcttcgag agccttcttt cccggcccca
     1021 gataggcaag agctttcatc gttgacattc ctgagttggg aagagggcac accaaaaccc
     1081 ctggatcgct ccaggggttc tgcacttccg aagaagaacc ttaatagaag acgccgtaat
     1141 cgacctgtac gatgtcgccg ctgtagcggt cgacgagcag cgcatcgttt ccgacgcgca
     1201 cccagacgag attgggcggc ggcgcaaaca ggccgaacat cataaaattg gtgatccagt
     1261 agtcacgaac gaaatagttg cgcggaagac ggttgccgtg gctccaacga cgggcctggt
     1321 agccctgcgg cgcgcgataa ctgccgttgt ggaaacggcg cggggcctga acgttgcgcc
     1381 gaagcgaatt gatgctgggc tggcgccccg cattgttgcc tgacatggag ttgccgcgcg
     1441 aattggtggt gacaccggcc ggcgaacgat tttgagtcct cgcgccgcgg gagttgacgg
     1501 cattcgtatt cgcacgggtc gcgcccgtca cggcgccgcc cgcggaacga ttaccgggcg
     1561 tcgcgccttg cggtctggcg gcgttggggt tcgtttgagt tcgacccgtc gcggcggcgc
     1621 ccgcggaacg attcccagg
//
LOCUS       DASWPU010000248         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_112424,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000248 DASWPU010000000
VERSION     DASWPU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112424"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..757
                     /locus_tag="VGC27_05345"
     CDS             371..757
                     /locus_tag="VGC27_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282029.1"
                     /translation="MRKDEPPSVLGEALLDAVVPRRKPKDYRPRRPAQPANTFVNRSG
                     EIASDMAAAGVSPSSAFALPSPNRSIGSAQAGKASTFALRASADKPASSTGQEGKSAK
                     DKGRGSFLIHEVQLGGGRGESNPENE"
     gene            951..>1559
                     /locus_tag="VGC27_05350"
     CDS             951..>1559
                     /locus_tag="VGC27_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282030.1"
                     /translation="MKPRAVKIAAACAVLFAVSQASSAEFLEQFKTVKVTADRQARTG
                     WQVFTGSGEAKATLTAKNHHALMTVDARRDRRNIYWALIKHSISDGIDRSLLGTPGRE
                     VRVEIKLRISDAPRRVHIQINHTRTTDVHATLREFDLPDTNWHVISYTTENFDAKPTD
                     DVFVSLGMTDMGRAIYTSEIEYVKADIVDPGKTGPDLGPPLRY"
BASE COUNT          324 a          490 c          473 g          272 t
ORIGIN      
        1 caccggacac taagtttcaa ctctcgcgtt tgcaattaat ggttgatatg gatgcagaaa
       61 ataagtggtc tgagtgagtt tctctgtaac gctgcaaacc atgggccgca ccgaggtggc
      121 gctcggcaag gagttgacga tttcgttccg gaacgcggtg tagtggacgc ttatcccttt
      181 tcctgcacgc cgagatggag cgccggttca aggcgttagc ggcgaaggtt acaacttatc
      241 cggcacgtcc tatccggccg tagaatgtgc ggttggtggc agtgatgccc ccttcgtcac
      301 ccttcgctcc gttggagctt cgggtgacac ttcccccgct tcgcgggggc agaaaaggaa
      361 ggagtgtgcc gtgcggaagg atgaaccacc ttcagtatta ggcgaagcgt tgctggatgc
      421 ggtagtgcca cgtcgcaagc caaaggatta tcggccgcgc cgccccgctc agcctgcgaa
      481 cacgttcgtc aatcgcagcg gagagatcgc gagcgacatg gcggctgccg gtgtttcccc
      541 ctcatccgcc ttcgccctgc cttcgccgaa tcggagcatc ggctccgcgc aggcaggcaa
      601 ggcctccacc ttcgccctcc gggcttcggc ggacaagccc gcttcttcca cagggcaaga
      661 gggaaaaagc gccaaggata aagggagggg gtccttcctt attcacgagg ttcaattggg
      721 cggcgggagg ggggagtcca atcctgaaaa tgaataagca cagaaggtgg aaagggcggc
      781 ggaacgatcc agatgttggt gcatcccccc tcccaccacc tcgccccgtt acggaagaag
      841 gaagaatgcc gcaaggacgg cggttggcgg tttggggttg aagcgataat gttaccgcta
      901 acaagaagtc cgcccgccac agggcaaatg cgttcaggga gtgccgctcg atgaagccgc
      961 gtgccgttaa gatcgccgcc gcgtgcgcgg tgctgtttgc cgtttcgcaa gcctcctccg
     1021 cggaattcct cgaacagttc aagaccgtca aggtgacggc cgaccgccag gcccgcaccg
     1081 gctggcaagt cttcaccggc tcgggcgaag ccaaggcgac gctcaccgcc aagaaccatc
     1141 acgccctgat gaccgtggac gcgcgccggg atcgccgcaa catctactgg gcgctgatca
     1201 agcactccat ttccgacggc atcgaccgca gcctgctggg aacgcccggc cgcgaagtgc
     1261 gcgtcgagat caagctgcgc atcagcgacg cgccgcgccg cgtccacatc cagatcaacc
     1321 acacccgcac caccgacgtg cacgccacgc tgcgcgagtt cgaccttccc gacacgaact
     1381 ggcacgtcat cagctacacg accgagaact tcgacgccaa acccaccgac gacgtgttcg
     1441 tcagtctggg catgaccgac atgggccgcg ccatctacac gagcgagatc gaatatgtga
     1501 aggccgacat cgtcgatccg ggcaagacgg gtcccgacct gggcccgccg ctgcgctac
//
LOCUS       DASWPU010000249         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_39125,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000249 DASWPU010000000
VERSION     DASWPU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39125"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            695..1627
                     /locus_tag="VGC27_05355"
     CDS             695..1627
                     /locus_tag="VGC27_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEY0282031.1"
                     /translation="MSAFVVVNPASGSTSREWRGVENALSAIYPYMSVAFTRARGDAA
                     ALVTNALREGHHEIVAVGGDGTINEVVNGFFDRGGAISPDAVLAFVCCGAAGDFKKAF
                     GIVETGVQAAEHLRHASVRAIDVGLVSFLSKTGMARTRAFANIASFGLSGEIADSVSR
                     AVVSRLFGAPFAFAFHSTLGLLTYRGRKVRLIVDDVYDEIVAISAVAVANGSWFGGGM
                     QVAPDAKPDDGLFDVVIFADAPKRRMLADMKFIYSGEHLGNPNVRVIRGRKVVAAPVA
                     ETRGRAVLIETDGEAVGRLPATFEVLPRALNVRC"
     gene            1644..>2293
                     /locus_tag="VGC27_05360"
     CDS             1644..>2293
                     /locus_tag="VGC27_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0282032.1"
                     /translation="MSIDRTKIRWNGWGWAAHKDVLGGREDVWTWLAGELGMPALLAT
                     PPRPLEELSLPAPALYVEDRKALDDIVGADRVRDGIYERAFHALGRSYYDLLRLRAGD
                     LSLAPDAVLYPRGTGEVLALLSLAAERGIAVIPYGGGTGMVGGVSAARGAFQSAITLD
                     LSAMDRLIEVDPVSATATAEAGITGPALEKALQAKGFTLGHRPESFEFSTLGGWIA"
BASE COUNT          415 a          706 c          737 g          435 t
ORIGIN      
        1 atctcaagct gatcccgaac ccgaagttgc agcgcaagac cgtggaacgg ctggaaaaga
       61 tcaaacaggg cgagcgggac ttttatttct agagtcgtat gccatcaagt cgatgcgcaa
      121 aaaacttgtt gtcatggccc gccaccccgg tccgcgcgaa gcgcggccga agtgtaaact
      181 tagtgcgggc cacccaggtg acgacggtac gacgccaaag gaacttcgct gagtctcttc
      241 ttcgacaatc tcgccacgtt tcaactggat ggcccgtatt cacgggccat gacaagcgaa
      301 tatttagttc cgtccgatca caagcggttc tagagcgcaa tccggcaaag tggtcggtag
      361 cagcagcgtg ccgacgcggt ttgccgtcag attgcgcgaa aaacaaggac actagcaacc
      421 tattgaatcc tagagcgttt tcaggaaaag tggaacccgg ttttccgtcc gaaaacgcga
      481 caaattaaga acttagagcc tttcagcgtt tccgtgaaac gatgaaaggc tctagtgtcg
      541 ttttggtttc gaagttcgct tgcaacccga tagtgcgcat ggtgcgaact tcgaaaccac
      601 gacactagat gatcgttatc ggaccacacc cgttccttga caagatgcca gccgcgccgg
      661 agcctctgct tcctattggt acgcggaacg ccgcatgagc gccttcgtcg tcgtcaatcc
      721 ggcttccggc agcaccagcc gcgaatggcg cggcgtggag aacgcgcttt cggcgattta
      781 cccttacatg tccgtcgcct tcacgcgggc gcgcggcgac gccgccgcct tggtgacgaa
      841 cgccttgcgc gagggtcatc acgaaatcgt cgcggtgggc ggcgacggca ccatcaacga
      901 ggtggtgaac gggttcttcg acaggggcgg cgcgatttct cccgacgccg tgctggcgtt
      961 cgtctgctgc ggcgcagccg gcgacttcaa gaaagcgttc ggcatcgtcg agaccggcgt
     1021 gcaggccgcc gaacatttga ggcatgccag cgtgcgcgcg atagacgtgg ggcttgtttc
     1081 gtttctgtcc aagaccggta tggcgcgcac gcgtgccttc gccaacatcg cctcgttcgg
     1141 cctgtcggga gaaatcgccg attccgtcag ccgggccgtc gtttcgaggc tgttcggcgc
     1201 tcctttcgcc ttcgcgttcc acagcacgct cggccttttg acctatcgcg ggcgcaaggt
     1261 ccgcctgatc gtggacgacg tttatgacga gatcgtcgcg atttcagccg tcgccgtcgc
     1321 caacggaagc tggttcggcg gcgggatgca ggtggcgccc gacgccaagc cagatgacgg
     1381 attgttcgac gtcgtcatct tcgccgacgc gccgaagagg cggatgctgg ccgacatgaa
     1441 gttcatctac agcggcgaac atctgggcaa tccgaatgtg cgggtgatcc gcggccgcaa
     1501 ggtggtcgcc gcacccgtcg ccgaaacccg cggccgggcg gtgctgatcg agaccgacgg
     1561 cgaagcggtg ggccgtctgc ccgccacttt cgaggttctg ccccgcgccc tgaatgtgcg
     1621 atgctgaccg gccgggaggg cgcatgagca tcgaccgcac gaaaatccgc tggaacggat
     1681 ggggctgggc cgcgcacaag gatgtgctgg gcggtcgcga ggacgtctgg acctggctgg
     1741 ccggcgaact gggcatgccg gccttgctgg cgacacctcc ccgtccattg gaagaattgt
     1801 cgcttccggc cccggcgctg tatgtcgagg accgcaaggc gctggacgat atcgtcggcg
     1861 cggatcgcgt gcgcgacggc atctacgagc gcgccttcca tgcgctcggc cgctcctatt
     1921 acgacctgtt gcggttgcgc gcgggcgatc tgtcgctggc gcccgacgcc gtgctctacc
     1981 cgcgcggaac cggggaagtg ctggcactat tgtcgctggc ggcggaacgc ggcatcgcgg
     2041 tcatcccgta tggcggcggt accggcatgg tgggcggcgt cagcgccgca cgcggcgcgt
     2101 tccagtccgc gatcaccctg gacctgtcgg cgatggaccg cttgatcgag gtcgatccgg
     2161 tctccgccac cgcgacggcc gaagcgggca tcaccggtcc ggcgctggaa aaagccttgc
     2221 aggcgaaagg cttcacgctc ggccatcggc cggaatcctt cgaattctcg accttgggcg
     2281 gctggatcgc tca
//
LOCUS       DASWPU010000250         7080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_87448,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000250 DASWPU010000000
VERSION     DASWPU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87448"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VGC27_05365"
     CDS             <1..180
                     /locus_tag="VGC27_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282033.1"
                     /translation="PLYIIDIGTGHAKRAGTLELVDGKVAFYLSDEDPPLTDEQKARV
                     AARLDAFQKALSKKN"
     gene            complement(221..2245)
                     /locus_tag="VGC27_05370"
     CDS             complement(221..2245)
                     /locus_tag="VGC27_05370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0282034.1"
                     /translation="MRSSLLAAAAALIASTTVLAAVPNMKAPLIERQKLFGNPSQSQG
                     RLSPDGKWFSWIAPSDGVMNIWVAPVADPATKRLLTAEKKRPIRSYFWSPDSASILYV
                     NDKDGDENFLLYGVNVSTGEQHTLTPFEKTRALVFGVSNHVKDRILVGLNNRDARWHD
                     VYSLDLKTNALTPVLTNTGEYAGFIADESLALRIATKSRPDGGSDFFHIAGNKVDEKP
                     FAGVGLEDSQTTGPAGYTADGKTLYWIDSRGRDTAALVAQDTKTGKTHIVAESPKADI
                     DGVLANPQTGYVQAYSVNYLKTEWTATDPAIKDDLDFLQSNLKGQIFVTSRTYADDLW
                     TVATDPVTAPSSAYLYNRKARTLTKLYVTRPELEGATLADMHPVEIKTRDGLTEVSYL
                     TLPPGSDSKGDGHPDHPLAMVLLVHGGPWGRDEYGYNSYHQWLANRGYAVLSVNYRGS
                     TGFGKSYISAGDLQWGAKMHDDLIDAVDWAIAGGITTRDKVAIMGGSYGGYATLVGLT
                     FTPDTFACGVDIVGPSNLATLLKTIPPYWEAVKVQFYQRMGDPTTEEGQKLLHDRSPL
                     FKADAIKKPLLIGQGANDPRVNQAESDQIVKAMSDKKIPVTYIVFPDEGHGFARPENN
                     IAFNAAAEQFLGKCLGGRSEPIGGTLKASTAQVPHGIEFTPGLKEAVAGK"
     gene            2368..3177
                     /locus_tag="VGC27_05375"
     CDS             2368..3177
                     /locus_tag="VGC27_05375"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY0282035.1"
                     /translation="MSDILNSILLGIVEGLTEFLPVSSTGHLLVAEWLLGLPDNHWEA
                     FTIVIQFGAILSVVAVYWRKFWDVLVGLPHSRAARQFAINVIVAFIPSAIFGALLIKY
                     INGYLLQPTFALPVIATTWIVGGILILVLERIAPKPRHFDGDHLPLSKALQIGFCQVI
                     SILPGISRSGATILGGELLGVERKAAAAFTFYLAVPTMFGATVFELYKKWDLMTAAAG
                     TNIAVGFVVSFLVAYVVVKTFIAFVGRYGLKPFGWYRIATGAALIAYLFAH"
     gene            complement(3442..5964)
                     /locus_tag="VGC27_05380"
     CDS             complement(3442..5964)
                     /locus_tag="VGC27_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY0282036.1"
                     /translation="MNDLVSRMTLEEKASQLVNQARAIPRLGVPAYNWWNEALHGVAR
                     NGIATVFPQAIGLGATFNPPLIHEMGVAISTEARVKYNQAGYSNNHGIFAGLTFWSPN
                     INIFRDPRWGRGQETYGEDPFLSGLMGKAFVTGMQGDDPKYLRTITTPKHYAVHSGPE
                     PTRHEVDVPASKHDMRDTYLPQFRTAIVDGKAGSVMCAYNRVNGQPACAHDFLLMETL
                     RKAWDFKGVVVSDCDAIADIERGHHFTKTYAEAAAVSLKTGVDNDCADFAIETTDSSD
                     YQRYIDAVKEGHVPESVVDESLRRLFTARMKLGMFDPKEMVKHAQVPDSELDSEAHGL
                     LALKAARESMVLLKNNGSLPLKQSVKRIAVIGPLADQVTPLLGNYNGTPSHPVTALEG
                     LKRQFPNAEITFEPGTNFLRAVEAVPPPVLTTDNGQPGLKAEFFGNEEFSGAAVMTRV
                     DANVDYTRDVGLMRNVPLPKVTPFAVRWTGTLTPTETGDYMVGLDTSTGRLSFDGKNL
                     VNIGGPGERTAKTALVHLEAGHKYAVKIERGFAHNLAIKLVWSRQIPDAAALAVAAAQ
                     KADVVVAVVGITSQLEGEEMVVEVPGFKGGDRVSIDLPKDEEDLLKAVRAATKKPLGV
                     VLYNGSALSVNWAAKNADAIVDAWYGGQAAGTAIAEVLSGAYNPAGRLPVTFYTGVEQ
                     LPPFEDYSMANRTYRYFKGKPLYSFGYGLSYAKFAYSGLQLSTPAVKAGDMLGVDVDV
                     KNVSDRDGDEVAQLYLAFPKVPGTPIRALRGVQRVHLKAGETQRLHFDLGPRDLSSVT
                     EAGDIVVQPGDYALSVGGGPRGATKAIVATGFKIDGEQKLAE"
     gene            complement(6262..>7080)
                     /locus_tag="VGC27_05385"
     CDS             complement(6262..>7080)
                     /locus_tag="VGC27_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEY0282037.1"
                     /translation="QLFKGNVRDEDSVTRALQGADAAVNLTGVLYIRGAQSFDALHAD
                     AARRIARLVREAGIKTLVHISAIGADPDAVSHYARSKGAGEKNLREEFPEATILRPSL
                     VFGPEDQFFNKFAWLARLSPVLPLIGGGRTKFQPVFVGDVAAAILRALSDPATRGKTY
                     ELGGPAVYTFKELLQLILRQTGRKRLLVPLPFFLASIKAFFLQLPSLFLPAAPLLTLD
                     QVRLLKTDNVVHEGALPLADLGIVPSAVEAIVPSYLWRFRAKGQFQEIAQKLSD"
BASE COUNT         1193 a         2207 c         2271 g         1409 t
ORIGIN      
        1 ccactttaca tcatcgacat tgggaccggc cacgccaagc gagccggaac gctggaatta
       61 gtggacggca aagttgcatt ctatctaagc gacgaagacc cgcccttgac ggacgagcag
      121 aaggcgcgcg ttgccgcgcg cctcgacgct ttccagaagg ctctgtccaa gaagaactga
      181 cgtctgctct accggaacgg ccgcgcgtcc ttccaggcct ctatttaccc gcaacagcct
      241 ccttcaggcc gggcgtgaat tcgataccgt ggggaacctg cgcggtggaa gccttcagcg
      301 tgccgccgat cggctccgac cgtccgccca ggcacttgcc caggaactgt tccgccgccg
      361 cgttgaaggc gatgttgttc tccggccggg cgaagccatg gccctcgtcg gggaagacga
      421 tgtaggtcac cgggattttc ttgtcggaca tcgctttgac gatctggtcg gattcggctt
      481 ggttgacgcg cggatcgttg gcgccctggc cgatgagaag cggtttcttg atggcatccg
      541 ctttgaacaa aggtgagcgg tcgtgcagca gcttctgtcc ttcttccgtc gttggatcgc
      601 ccatgcgctg gtagaactgg accttgaccg cttcccaata gggcggaatg gtcttcagca
      661 atgtggcgag gttcgacggc cccacgatat ccacgccgca ggcgaaggta tcgggcgtga
      721 aggtcaggcc gaccagcgtg gcatagccgc cataggagcc gcccatgatg gcgaccttgt
      781 cgcgcgtggt gatgccgccc gcgatcgccc agtccaccgc gtcgatcaga tcgtcgtgca
      841 tcttggcgcc ccactggagg tcgccggcgg agatgtagga tttgccgaag cccgtcgagc
      901 cgcgatagtt caccgacagc accgcataac cgcgattggc gagccactgg tgatacgaat
      961 tgtagccgta ctcgtcgcgg ccccacggcc cgccatgcac caacagcacc atcgcgagcg
     1021 ggtgatcggg gtggccgtcg cctttggaat cgctgccggg cggcagcgtc aggtaggaca
     1081 cttcggtcag cccgtcgcgg gtcttgattt ccaccggatg catgtccgcg agcgtcgccc
     1141 cttcaagctc tggccgcgtg acgtagagct tggtcagggt gcgggccttg cggttgtaaa
     1201 gataggcgct cgacggcgcc gtcacggggt ccgtggcaac cgtccacaaa tcgtccgcat
     1261 aggtgcggga ggtgacgaag atctggcctt tcagattcga ttgcaggaaa tcgaggtcgt
     1321 ccttgatcgc gggatcggtc gccgtccact cggtcttgag gtaattcacg ctgtaggcct
     1381 ggacatagcc cgtctgcggg ttggcgagaa cgccgtcgat gtcggctttc gggctttcgg
     1441 cgacgatgtg cgttttgccg gtcttggtgt cctgcgcgac gagggccgcc gtgtcgcggc
     1501 cgcgcgagtc gatccagtag agcgtcttgc cgtccgcggt gtagcctgcg gggcccgtgg
     1561 tttgcgagtc ttccagtcca acgccggcga acggcttttc gtccacttta ttgccggcga
     1621 tgtggaagaa gtccgacccg ccgtccggcc gcgattttgt cgcgatgcgc agcgccaggc
     1681 tttcgtcggc gataaagccc gcatattcgc cggtgttcgt caggaccggc gtcagcgcat
     1741 tggtcttcag gtcgagactg tagacgtcgt gccagcgcgc gtcgcggttg ttgaggccga
     1801 ccagaatgcg gtccttgacg tggttgctga cgccgaagac cagggcgcgg gtcttttcga
     1861 acggagtgag ggtgtgttgc tcgcccgtgc tgacgttcac gccgtagagc aggaaattct
     1921 cgtcgccgtc cttgtcgttc acgtacagga tggaagcgga atcgggcgac cagaaatagc
     1981 tgcggatcgg gcgtttcttt tccgcggtca gaagccgttt cgtcgccggg tcggcgaccg
     2041 gcgccaccca gatgttcatc acgccgtcgc tcggcgcgat ccaggagaac catttgccgt
     2101 cggggctgag gcggccctgc gactggctgg ggttgccgaa cagcttctga cgctcgatca
     2161 ggggcgcctt catgttgggc acagcggcga gcacggtcgt actcgcgata agagccgcgg
     2221 cggcggccaa gagcgatgat ctcatgcgtt ttccccaagg atggacggcg gcgcatcttg
     2281 gcacaaaagt gcgcgcattg aaacaaaagg agcatagccc gggacgaaga atgtgctacg
     2341 tctgccgcaa ttcttctggg gctattcatg tccgatatac tgaattcgat cctgctcggc
     2401 atcgtcgaag gattgaccga attcctgccc gtttcctcca ccgggcatct gctggtcgcc
     2461 gagtggctgc tcggtttgcc cgacaatcat tgggaagcct tcaccatcgt catccagttc
     2521 ggcgcgatcc tatcggtggt cgcggtctat tggcgcaaat tctgggacgt gctggtcggc
     2581 ctgccgcatt cgcgcgcggc gcggcagttc gcgatcaatg tgatcgtcgc gttcatcccc
     2641 tcggccatct tcggcgcgtt gctgatcaaa tacatcaacg gctacctgtt gcagccgacc
     2701 ttcgcgctgc cggtgatcgc gaccacctgg atcgtgggcg gcattctgat cctggtgctg
     2761 gagcgcatcg cgcccaagcc gcgccatttc gacggcgacc acctgccctt gtccaaggcg
     2821 ctgcagatcg gtttctgtca ggtgatctcg atccttcccg gcatctcgcg ctcgggcgcg
     2881 acgatcctcg gcggcgaact gctcggcgtg gagcgcaagg ccgccgccgc cttcaccttc
     2941 tatctcgcgg tgccgacgat gttcggcgcg acggtgttcg agctttacaa gaaatgggat
     3001 ttgatgacgg cggccgccgg tacgaatatc gccgtgggtt tcgtggtctc gttcctggtg
     3061 gcgtatgtgg tggtgaagac cttcatcgcc ttcgtcggcc gctacggcct caagccgttc
     3121 ggctggtacc ggatcgcgac gggcgcggcg ctcattgcgt acctgttcgc acactaaggc
     3181 tgctttgaat tgaattgact cgcgagtcta ttcttgtcat ggcccgccac agcgggccat
     3241 ccaagtgatg acgagcgcgc ggcaacaaaa ggggctcagt gcattcttat aagttcagtg
     3301 aagcgtcatc tgggtggccc gcattcgcgg gccatgacaa tcgagttgag tgagcaaacc
     3361 caggacaatc ttgtcccaga tcgcggagcc gaagagacac gggcccgggc aacgcccagg
     3421 cccgcgtgag atacttgttt tctactccgc cagcttttgc tctccgtcga tcttgaagcc
     3481 cgtcgcgacg atggctttgg tcgcgccgcg cgggccgccg ccgacgctga gggcatagtc
     3541 gccgggctgc accacgatgt cgcccgcttc cgtcaccgag ctgagatcgc gcggcccgag
     3601 gtcgaaatgc aggcgctgcg tttcgcccgc cttcaaatgg acgcgctgga cgcccctgag
     3661 cgcgcggatc ggcgtgccgg gcaccttcgg gaaggccaga tagagctggg ccacttcgtc
     3721 accgtcgcgg tcgctgacgt tcttcacgtc cacgtccacg cccagcatat cgcccgcctt
     3781 cactgccggc gtcgagagct gcaacccgct ataggcgaac ttcgcgtagc tcaggccgta
     3841 gccgaaggaa tagagcggct tgcccttgaa gtagcggtag gtacggttgg ccatcgagta
     3901 gtcctcgaac ggcggcaact gctccacgcc ggtgtagaac gtcacgggca gccgcccggc
     3961 cggattgtat gcgccggaaa gcacttcggc gatggcggtt ccggccgctt ggccgccgta
     4021 ccaggcatcg acgatcgcgt ctgcgttctt tgcggcccag ttcaccgaca gcgcgctgcc
     4081 gttatagaga accacgccca gcggcttctt ggtcgccgcg cgcaccgctt tcaggagatc
     4141 ctcttcgtcc ttcggcaagt cgatgctcac acggtcgccg cccttgaagc ccggcacctc
     4201 gacgaccatc tcctcgcctt cgagctgcga ggttatgccc accaccgcga cgacgacgtc
     4261 ggccttttgg gccgccgcca cggccagcgc cgcggcgtcc gggatctgcc tggaccacac
     4321 cagcttgatc gcgaggttgt gcgcaaagcc gcgctcgatc ttcaccgcgt atttgtggcc
     4381 ggcctcaaga tggaccaagg cggtcttggc cgtgcgttcg cccggcccgc cgatattgac
     4441 cagattcttc ccatcgaacg acagacgccc ggtcgacgta tcgagcccca ccatgtagtc
     4501 gcccgtttcc gtgggcgtta atgtgcccgt ccagcgcacc gcgaacggag tgactttggg
     4561 cagcggcacg ttgcgcatca agccgacgtc acgggtgtaa tcgacattgg catcgacgcg
     4621 ggtcatgacc gctgcgcccg agaattcttc gttgccgaag aactccgcct tgaggccggg
     4681 ctgaccgttg tcggtcgtca gcaccggcgg cggcaccgct tcgaccgcgc gcaggaagtt
     4741 ggtgcccggc tcgaaggtga tctcggcgtt cgggaactgg cgcttgaggc cttccagcgc
     4801 cgtcaccgga tgcgacggcg tgccgttgta attgccgagc agcggcgtga cctgatccgc
     4861 caggggaccg atcaccgcga tgcgcttgac gctctgcttg agaggcagcg agccgttgtt
     4921 tttgagcagc accatggatt cgcgcgccgc cttgagcgcc aagagaccgt gggcttcgga
     4981 atcgagttcg gaatccggca cctgggcgtg cttcaccatc tccttcggat cgaacatgcc
     5041 gagcttcatc cgcgccgtga ataaccgcct gagcgattca tcgaccaccg attccggtac
     5101 gtggccttct ttcacggcgt cgatgtagcg ctggtaatcg gaactgtccg tggtttctat
     5161 cgcgaagtcc gcgcagtcgt tgtccactcc ggtcttcagc gacacggccg ccgcctcggc
     5221 ataggtcttg gtgaaatggt ggccgcgctc gatgtcggcg atggcgtcgc agtcggaaac
     5281 gacgacgccc ttgaagtccc atgccttgcg cagcgtctcc atcaggagga aatcgtgggc
     5341 gcaggcgggc tggccgttga cgcggttgta ggcgcacatc accgagccgg ccttgccgtc
     5401 cacgatcgcg gtgcggaact gcggcagata ggtgtccctc atgtcgtgct tggaggcggg
     5461 cacgtcgact tcgtggcgtg tcggttcggg accggaatgc accgcatagt gcttgggcgt
     5521 cgtgatcgtg cgcagatatt tcggatcgtc gccctgcatg ccggtgacga acgccttgcc
     5581 catcaggccc gacaggaacg gatcttcgcc gtacgtctcc tggccgcggc cccagcgcgg
     5641 atcgcggaag atgttgatgt tgggcgacca gaaggtcagg cctgcgaaaa tcccgtggtt
     5701 gtttgaatag ccggcttgat tgtatttcac gcgcgcttcg gtcgagatcg ccacgcccat
     5761 ctcgtggatc agcggcggat tgaaagtggc acccaggccg atggcctgcg ggaacacggt
     5821 cgcgatgccg ttgcgcgcca cgccgtgcag cgcctcgttc caccaattgt acgccggcac
     5881 gccgagccgc gggatcgcac gcgcctggtt gacgagttgt gaagctttct cctccagcgt
     5941 catgcgcgag acgagatcgt tcacccgcgc ctcgatgggc aattgcggat tcgggtaaat
     6001 cggggcggat gtagaagcgc tcccattcgc ggccgcgttt gccggtccgg cggcgcttga
     6061 tatcgccccc aagagggcga gggcggcgac tgtcaacgga gtgcggaaat tggtaccaag
     6121 ccacttcatg gcgatcttcc tccagggcgc gggatcgatg gtgcgattag ccctataccg
     6181 cttgggaaac ttccgcaatc gggcggcagc gcatactctt ccggttgtaa aaaacaccag
     6241 ttacgaggcc tggcggcaac tctaatccga aagcttttgc gcgatttcct gaaattgccc
     6301 cttggcccgg aagcgccaca gatagctcgg cacgatggct tccacagcgc tgggtacgat
     6361 gccgaggtcg gcaagcggca gcgcgccttc atgcacgaca ttatcggttt tcagcaggcg
     6421 cacctgatca agcgtgagca gcggagcggc cggcaggaac aggctcggca actgcaggaa
     6481 gaacgccttg atggacgcaa ggaagaacgg cagcggcacc agcagccgct tgcggccggt
     6541 ttggcgcagg atcagttgca gcaattcttt gaaggtgtag accgccggtc cgccaagctc
     6601 gtaggtcttg ccgcgcgtcg ccggatcgct cagcgcccgc aggatcgccg ccgccacgtc
     6661 gccgacgaac accggctgga atttcgtgcg cccgccgccg atcaagggca gcaccggcga
     6721 gaggcgcgcc agccacgcga acttgttgaa gaactggtct tccggcccga acaccaatga
     6781 cggccgcagg atcgtggcct cgggaaattc ctcgcgcagg ttcttttcgc ccgcgccttt
     6841 ggaccgggcg taatgcgata cggcatccgg gtccgcgccg atggccgaga tatggacgag
     6901 cgttttaatt cctgcctcgc gcaccaaacg tgcgatccgc cgggcggcgt cggcatggag
     6961 cgcatcgaag ctctgcgcgc cgcggatgta gagcacgccc gtcagattca ccgccgcgtc
     7021 ggcgccttgg agcgcgcgcg tcacggaatc ctcgtcgcgg acgttgccct tgaagagctg
//
LOCUS       DASWPU010000251         5689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_81635,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000251 DASWPU010000000
VERSION     DASWPU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81635"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VGC27_05390"
     CDS             complement(<1..279)
                     /locus_tag="VGC27_05390"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEY0282038.1"
                     /translation="MHTPTMAQSRDKEQPQRRNWVSPLAPPATAAAEAAFARAGFSDP
                     TLVLRWEEIAGPETARLARPVRFSQGPNGGVLTLKAEPGAALFLQHETR"
     gene            274..1458
                     /locus_tag="VGC27_05395"
     CDS             274..1458
                     /locus_tag="VGC27_05395"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY0282039.1"
                     /translation="MHGSHQSRQSRRNNHAQRLLAWYDVHRRELPWRAKPGEAADPYR
                     VWLSEIMLQQTTVQAVASYYRKFIARWPTVGALAGAPLDEVLAAWAGLGYYARARNLH
                     KAAKLVAGTGRFPSTIDELRKLPGVGAYTAGAIAAIAYGAREAAMDANAERVIARLFA
                     VEEPLPGSKAKLLVLGKSLVPRRRAGDFVQALMDLGAGICTARRPACGDCPWTDECEG
                     RKRGIAESLPAKAPKRTRPLRRGAAFVARDGRGAVLLVKRPEKGLLGGMLQPLLGPWG
                     TKFPAAKDALAQAPFPAAWKKLPGFVRHTFTHFELEIEVWTAEIFNHPKLSWPATAGH
                     PGDNAIVMKERLDGRVKPGHDELEAKWLPAAKLKDAALPTVMRKIIAHAFEDQAKSAR
                     RR"
     gene            complement(1431..2522)
                     /locus_tag="VGC27_05400"
     CDS             complement(1431..2522)
                     /locus_tag="VGC27_05400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY0282040.1"
                     /translation="MSELKLEDALDRVIEGDCVERMRALPEGLVDLIFADPPYNLQLK
                     NELRRPDNSKVDAVDDHWDQFSSFAEYDRFTREWLTEARRVLKDSGALWVIGSYHNIF
                     RVGTILQDLGFWVLNDVVWRKSNPMPNFRGRRFTNAHETLIWAAKSAGQKSYTFNYEA
                     MKALNDDLQMRSDWTLPICSGGERLKDGDGQKAHSTQKPESLLYRVILSSSKQGDVVL
                     DPFFGSGTTGAVARKLGRHFIGIERDKDYASLARSRIAEIEPADKEAIETTKSKRAEP
                     RIPFGWVVERGLLPPGTVLQGHQKNQRAKVRADGTLVCADATGSIHRIGAHVQGLDAC
                     NGWTFWQFELNGKLVPIDVLRQRLRADLA"
     gene            complement(2614..3243)
                     /locus_tag="VGC27_05405"
     CDS             complement(2614..3243)
                     /locus_tag="VGC27_05405"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY0282041.1"
                     /translation="MPNYAFESRALARIAGLVAGVDEAGRGPLAGPVVAAAVIFEKKR
                     IPNGLDDSKKLTPRRREELYAQIYKFAVAVGVGEASVDEIDLVNIRQATHLAMLRAVR
                     ALAPAPAFALVDGNDAPALPCPCQTIIGGDGRSVSIAAASIIAKVTRDRMMAALHELH
                     PEYGWAGNKGYGTEAHLLALKRYGATPHHRRSFAPVHNMLCVWNPVDST"
     gene            complement(3265..3555)
                     /locus_tag="VGC27_05410"
     CDS             complement(3265..3555)
                     /locus_tag="VGC27_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282042.1"
                     /translation="MNETIDDLLRQMPVVPDDGFSRRVMAGVRAMERRRMFVTAAAVA
                     LGIVLALLILPLREIGVELGAGIPKIASSAALGLAMAAMILTLLLDREFSRL"
     gene            complement(3573..4088)
                     /locus_tag="VGC27_05415"
     CDS             complement(3573..4088)
                     /locus_tag="VGC27_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0282043.1"
                     /translation="MSEGDWLAREAAAGDTAAFGVLVRQHQGKLRGFLLRLTRGNHAL
                     ADDLAQETFLEAFRKIAQFGSGSFFGWLCAIAYSRYLMEARKRKLESLDEAEDVADDV
                     PEAERASGIRLDLEKAFARLAPAQRAALTLCFALGFSNEEAAAALHIPLGTLKSHVNR
                     GRDKLSKMLGA"
     gene            complement(4108..4497)
                     /locus_tag="VGC27_05420"
     CDS             complement(4108..4497)
                     /locus_tag="VGC27_05420"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEY0282044.1"
                     /translation="MFYGWDTDGIVFWGAIVLIVLISGYFRHRARIAKYKMMETLAEK
                     GQTLSPELLASLANGNGDRRRNPIQSGIFLICIGIALAVFFWATGGAGNPFAGEHVPW
                     LAVIGIFPFMIGLARLLGGLTEKRRDN"
     gene            complement(4575..4871)
                     /locus_tag="VGC27_05425"
     CDS             complement(4575..4871)
                     /locus_tag="VGC27_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282045.1"
                     /translation="MRTSDGTNSGSRIRRLVRADIFQMCIGLVLAVFLVQSDRAISSF
                     GAAGAPPALISKDAIVAGAHMVHLECVRLVTAFNNVVRVTKSGSTPCHFIAIFE"
     gene            complement(4941..>5689)
                     /locus_tag="VGC27_05430"
     CDS             complement(4941..>5689)
                     /locus_tag="VGC27_05430"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY0282046.1"
                     /translation="AAELLAKELSAPGYVPKVIAMGTNTDPYQPLEKKMRITRSILEV
                     LREFRHPVAIVTKSPLILRDTDILGEMAEMGLARVALSVTTLDRKLARAMEPRAGTPT
                     RRLEAIKALSGAGIPTAVMFAPVIPALNDNEMEAVLAAAKEAGARSAGYVLLRLPLEI
                     KDLFHEWLEANRPDSAKHIISLIRQMRGGKEYDARWHLRMRGTGPYAEMIARRFHMAV
                     KRLELNKDSKPLRTGLFKSPPRPGDQLALF"
BASE COUNT         1016 a         1784 c         1879 g         1010 t
ORIGIN      
        1 gcgggtttca tgctgcagga acagggccgc tcccggctcg gccttgagcg tcaggacgcc
       61 gccgttaggc ccttgggaaa agcggaccgg ccgggccagc cgggcggttt cgggaccggc
      121 gatttcttcc cagcgcagga ccagcgtcgg gtcggaaaag cccgcgcgcg cgaaggcggc
      181 ttcggctgcc gcagtcgcag gcggcgccaa gggcgacacc cagttgcgcc gttgcggttg
      241 ttccttgtcg cgggattggg ccatggtggg agtatgcacg gttcccacca gtccaggcaa
      301 agccgccgga acaatcacgc acagcgattg ttggcctggt acgacgttca ccgccgcgag
      361 ctgccctggc gcgccaagcc gggtgaggcc gccgatccct accgcgtctg gctgtccgag
      421 atcatgttgc agcagacgac cgtacaggcc gtcgcctcct attaccgaaa attcattgcg
      481 cgctggccga cggtgggggc gctggccgga gcgcccttgg acgaagtgct ggccgcctgg
      541 gctgggctgg gctactacgc gcgggcccgc aatctgcaca aagcggcgaa gctggtggcg
      601 gggacggggc ggtttccatc caccattgat gaattgcgca agctgccggg ggtgggggcc
      661 tacacggcgg gagccatcgc cgccatcgcc tatggcgcgc gcgaagcggc catggacgcc
      721 aacgccgagc gggtaatcgc gcggctgttc gcggtggaag aaccgttgcc cggctccaaa
      781 gccaagcttc tagtgttggg caagtctctg gtaccgcgcc ggcgggcagg ggatttcgtc
      841 caggcgctga tggacctggg cgcgggcatc tgcacggcaa ggcggccggc ttgcggggat
      901 tgtccgtgga cggatgaatg cgaggggcgc aagcgcggca tcgccgagag cttgcccgcg
      961 aaagcgccca agcggacgcg cccgctgcgc cggggcgcgg ccttcgtggc gcgcgacggg
     1021 cgcggagcgg tgctgctggt caagcggccg gagaaagggc tgctcggcgg catgctgcaa
     1081 ccgctgctgg ggccatgggg cacgaagttt cccgcagcga aagatgcgct ggcacaggct
     1141 ccgttccctg cggcatggaa gaagctgccc ggtttcgttc gccacacctt cacgcatttc
     1201 gaactggaga tcgaagtgtg gacggcagag attttcaacc atcccaagct gtcatggccc
     1261 gcgactgcgg gccacccagg tgacaatgcc attgtgatga aagaaagact ggatggccgg
     1321 gtcaagcccg gccatgacga actagaggct aaatggcttc cggcggcgaa attgaaagac
     1381 gccgccctgc ccaccgtcat gcgcaagatc atcgcgcacg cgttcgagga tcaggccaag
     1441 tccgcccgca ggcgctgacg caacacgtcg atcggcacca gcttcccgtt gagttcgaat
     1501 tgccagaagg tccagccgtt gcaggcgtcg agaccctgca catgggcgcc gatgcgatgg
     1561 atcgagccgg tggcgtcggc gcagaccagc gtgccgtcgg cgcgaacctt ggcgcgctgg
     1621 ttcttctgat ggccttgcag caccgtgccc ggcggcagga ggccgcgctc gaccacccag
     1681 ccgaagggaa tgcgcggttc ggcgcgcttg gatttggtcg tctcgatcgc ttccttgtcg
     1741 gccggttcga tctcggcgat gcgcgagcgc gccagactgg cgtagtcctt gtcgcgttcg
     1801 atgccgatga aatgccgccc cagcttgcgc gccaccgcgc cggtcgtgcc ggagccgaag
     1861 aacgggtcga gcaccacgtc gccttgcttg ctcgacgaca ggatgacgcg atagagcagg
     1921 ctttccggct tctgggtcga atgcgccttc tggccgtccc cgtccttcag ccgttcgccg
     1981 ccggagcaga tcggcagggt ccagtccgag cgcatctgca aatcgtcgtt cagcgccttc
     2041 atggcttcgt aattgaaggt gtagcttttc tggcctgcgc tttttgccgc ccagatcagg
     2101 gtctcgtgcg cgttggtgaa tctgcggccg cggaagttcg gcatcggatt gctcttgcgc
     2161 cagaccacgt cgttgagcac ccagaagccg aggtcctgca ggatcgtgcc gacgcggaaa
     2221 atgttgtgat aggagccgat cacccacagc gcgccgctgt ctttcagcac gcggcgcgct
     2281 tcggtcagcc attcgcgggt gaagcggtca tattcggcga agctggagaa ttggtcccag
     2341 tgatcgtcga cggcatcgac cttggaattg tccgggcggc gcagctcgtt cttgagctgg
     2401 aggttgtaag gcgggtcggc gaagatcaga tccaccaagc cttcgggcag cgcccgcatg
     2461 cgttccacac aatcgccctc gatcacccgg tcaagtgcgt cttccagttt gagttcgctc
     2521 atttgccgcc cgctttatcc acaaagaacg gcgacagtga ttcgtgtgag tccacacgtc
     2581 aagagtctta tgaatcaaag cgttataggg gattcaggtt gagtccacag gattccatac
     2641 acacaacatg ttgtggacgg gtgcaaagct gcggcggtgg tgaggggtgg ctccgtaccg
     2701 cttcagcgcc aaaagatgcg cttcggtgcc ataacccttg ttgccggccc agccatattc
     2761 ggggtgcaat tcgtgcagcg ccgccatcat ccggtcgcgc gtcaccttgg cgatgatcga
     2821 cgccgcggcg atggaaaccg agcgtccgtc gccgccgatg atggtctggc acgggcaggg
     2881 cagcgccggc gcgtcgttgc cgtccaccag cgcgaaggcc ggcgcgggag cgagcgcgcg
     2941 cacggcgcgg agcatcgcca gatgcgtcgc ctgacggata ttgaccagat cgatttcatc
     3001 cacgctcgct tcgccgacgc cgacggcaac cgcgaactta tatatttgcg cataaagttc
     3061 ttcgcgccgg cgcggcgtca gcttcttgga atcgtcgaga ccgttgggaa tgcgtttctt
     3121 ctcgaagatc accgcggcgg cgacgaccgg ccccgccagc ggcccgcgcc cggcttcgtc
     3181 cacgcccgcg accaagcccg caatgcgggc cagcgcgcgc gactcgaaag cataattcgg
     3241 catgaggcgt ttatagcgcg gcggctacag acgggagaac tcccggtcga gaagaagcgt
     3301 gaggatcatc gccgccattg caaggccgag cgccgcggaa cttgcgatct tgggaatgcc
     3361 ggcgccgagt tctacgccga tctcgcgcag aggcaggatc agcagcgcca gcacgatgcc
     3421 gagggcgacg gccgccgccg tgacgaacat ccggcggcgc tccatcgcgc ggacgccggc
     3481 catgacgcgc cgcgagaacc cgtcgtcggg caccacgggc atttgcctca gcagatcgtc
     3541 gatcgtctcg ttcatgacac acccattata cgctacgcac ccagcatttt ggataattta
     3601 tcgcgtccgc ggttaacatg cgatttcaga gttccgagcg ggatatgcag cgccgccgcg
     3661 gcttcctcgt tggaaaagcc gagcgcgaag cacagcgtca gcgccgcgcg ctgcgccggg
     3721 gcaaggcgcg cgaacgcctt ttcgagatcg agcctgatgc cgctggcgcg ttccgcttcg
     3781 ggcacatcgt ccgctacgtc ttccgcttcg tccaggcttt ccaatttgcg tttgcgcgcc
     3841 tccatcagat agcgcgaata ggcgatggcg cagagccagc cgaagaacga tccggagccg
     3901 aactgcgcga tcttgcggaa ggcttcgaga aacgtttcct gcgccagatc gtcggccagc
     3961 gcgtgattgc cgcgcgtcag gcggagcagg aagccgcgca gtttgccctg gtgttggcgc
     4021 accagaacgc cgaaggccgc ggtgtctccc gcggcggctt cgcgcgccag ccaatcgccc
     4081 tcgctcatga tcgttcagca taacacgtca attgtcccgg cgcttctccg tcaggccgcc
     4141 gagcagacgg gccaacccga tcatgaacgg gaagatgccg atcacggcaa gccaagggac
     4201 atgctcgccc gcgaacggat tgccggcgcc gcccgtcgcc cagaagaaga ccgccagcgc
     4261 gatgccgatg cagatcagga agataccgga ctggatcggg tttctacgcc ggtcgccatt
     4321 gccgttagcg aggctggcca gcaactccgg ggaaagggtc tggcctttct cggcgagcgt
     4381 ctccatcatt ttatatttgg cgatgcgcgc gcggtgccga aaataaccgc ttatcaaaac
     4441 gatcaggacg attgcgcccc agaaaacgat gccgtcggta tcccagccgt aaaacatctc
     4501 aagttcctcg cagataaagt ttcatcgaca agatgcggcc gagggccggg ttggatgcgg
     4561 gcgcggcgcc ggattcattc gaagatcgcg atgaaatgac aaggcgtcga gccggacttg
     4621 gtgaccctta cgacattgtt gaaggcagtg accagacgga cgcactcaag atgaaccatg
     4681 tgagcgccgg cgacgatcgc gtccttgctt atgagcgcgg gcggcgcacc ggcggcaccg
     4741 aacgaagaga tcgcgcggtc gctttgaacc agaaacaccg ccagcaccag gccgatgcac
     4801 atctggaaga tgtcggcccg gaccaatctt ctgatccggc tgccgctgtt tgtgccgtcg
     4861 cttgtgcgca tctggtaccc cgtcaaggct ctgcttccac ccctgagatg cagccaaggg
     4921 cgggtttgga tgcaggcgac ctagaaaagc gcaagctggt cgccggggcg gggcggggac
     4981 ttgaacaggc cggtacgcag cggtttggaa tccttgttca gctccaggcg cttgaccgcc
     5041 atgtgaaaac gccgcgcgat catttcggca taaggcccgg tgccgcgcat ccgcaggtgc
     5101 cagcgggcgt cgtattcctt gccgccgcgc atctgacgga taagcgagat tatgtgcttt
     5161 gcgctgtcgg gccgattcgc ttccagccat tcgtggaaca gatccttgat ctccagcggc
     5221 aggcgcagca gcacatagcc ggccgagcgc gcaccggcct ctttggcggc ggcgaggacg
     5281 gcttccatct cgttgtcgtt cagcgccggg atcacgggcg cgaacatcac ggcggtggga
     5341 atgcccgcgc cgctcagcgc cttgatcgct tccaggcggc gcgtgggggt tccggcgcgc
     5401 ggctccatcg cccgcgccag cttgcgatcc agcgtggtga ccgacagcgc cacgcgcgcc
     5461 agccccatct cggccatttc gcccaggatg tccgtatcgc gcaggatcag cggcgatttg
     5521 gtgacgatgg cgacgggatg gcggaattcg cgcaacacct ccaggatgga gcgggtgatc
     5581 cgcatcttct tttccagcgg ctggtaggga tcggtgttgg tgcccatcgc gatcaccttc
     5641 ggaacatagc cgggcgcgga caattccttc gccagaagtt cggcggcat
//
LOCUS       DASWPU010000252         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_69682,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000252 DASWPU010000000
VERSION     DASWPU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69682"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..808)
                     /gene="rplA"
                     /locus_tag="VGC27_05435"
     CDS             complement(116..808)
                     /gene="rplA"
                     /locus_tag="VGC27_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY0282047.1"
                     /translation="MTVLAKKVKTWKAKLVPGKAYPIDEAVGLVKEFAAAKFNESVDV
                     AVNLGIDASKSDQQVRGSTVMPHGIGKSVRVAVFTSGKNQDAARAAGADIVGLEDLAE
                     QVKAGKMDFDVVIASPDAMRIVGQLGQILGPRGLMPNPKVGTVSPDVAGAVKNAKSGQ
                     VRYRVDKAGIVHCTIGKAAFEPTALRENLQALINDLTRAKPASSKGIYLKRVTLSSTM
                     GPGVIVDHSSLD"
     gene            complement(820..1251)
                     /gene="rplK"
                     /locus_tag="VGC27_05440"
     CDS             complement(820..1251)
                     /gene="rplK"
                     /locus_tag="VGC27_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY0282048.1"
                     /translation="MAKKVQGYVKLQVPAGSANPSPPIGPALGQQGVNIMEFCKQFNA
                     QTQKLEKGLAIPVIITVYSDRSFTFIMKTPPASVLIRKAIGIEKGSGTPNTSKVGKIS
                     RKQLEEVARTKMPDLTAADLEAAVRTMAGSARSMGVDVEGL"
     gene            complement(1356..1889)
                     /gene="nusG"
                     /locus_tag="VGC27_05445"
     CDS             complement(1356..1889)
                     /gene="nusG"
                     /locus_tag="VGC27_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY0282049.1"
                     /translation="MAMRWYVVHAYSNFEHRVSESLKDRIKRDGLEDKFGEILVPTEE
                     VVEMREGQKRRSERKFYPGYVLVQMEMDEQTWHLVRDVPKVLGFIGGTSDKPAPISEK
                     EANQILRRVEEGVDKPRPKVLFEAGQVVRVTDGPFNDFNGVVESVNYEKSRLQVAVQI
                     LGRSTPVELDFSQVEKA"
BASE COUNT          321 a          646 c          527 g          396 t
ORIGIN      
        1 caccatctgc gcaggccggc gtaattaagg acttcgtacc cgttgccggg actcatccgc
       61 ctgcggtctt cgatgtaccg gcgaacctca cggcccgccg acacacatca aaacttcaat
      121 ccagactcga atgatcgaca atcacgcccg gacccatggt cgaggacagc gtgacgcgct
      181 tcaggtaaat gcccttggag ctcgccggct tggcgcgcgt caggtcattg atcagcgcct
      241 gcaggttttc acgcagcgcg gtgggctcga aagccgcctt gccgatcgtg caatgcacga
      301 tgccggcctt gtccacgcga tagcgcacct ggcccgactt ggcgttcttc accgccccgg
      361 ccacgtccgg cgacaccgtt cccaccttgg ggttgggcat caggccgcgc ggtcccagga
      421 tctgtcccag ctggcccacg atgcgcatgg catcggggct ggcgatcacc acatcgaaat
      481 ccatcttgcc ggccttcacc tgctcggcga ggtcctcgag gccgacgatg tcggcgcctg
      541 cggcccgggc tgcatcctgg ttcttgccag aggtgaacac ggcgacgcgc acgctcttgc
      601 cgatgccatg gggcatcacg gtggagccgc gcacctgctg gtcggacttg gaggcatcga
      661 tgcccaggtt cacggcgacg tccacggatt cgttgaactt cgcggcggcg aattccttga
      721 ccaggcccac ggcctcgtca atcggatacg ccttgcccgg caccagcttg gccttccagg
      781 tcttcacttt cttggcgagt acagtcattt cagttcccct caaaggcctt cgacatcaac
      841 gcccatgctg cgggcgctgc ctgccatggt gcgaacggcg gcttccaggt ctgccgcggt
      901 caggtccggc atcttcgtcc tggcaacttc ttcaagctgc ttgcgcgaaa tcttaccgac
      961 cttcgaggta ttcggagttc cactgccctt ctcgatgccg atggccttgc ggatcagcac
     1021 cgacgccggc ggcgtcttca tgatgaaggt aaagctgcgg tccgagtaca ccgtgatgat
     1081 cacgggaatc gccaggccct tctccagctt ctgggtctgc gcattgaact gcttgcagaa
     1141 ctccatgatg ttgacgccct gctgacccag cgcaggaccg attggcggcg acggattcgc
     1201 agatcccgcg gggacctgca gcttcacgta gccctgtact ttcttggcca tcttctactc
     1261 cttgggtgca aacgccgtta ggctccccaa tcagttaatc ctcggcgtat cgggcttagc
     1321 ccgatacgcc gttgaacccc ggccgtgagg ccgtgttaag ccttctcgac ctgcgagaaa
     1381 tccagttcga ccggtgtcga gcgaccgaga atctgcacgg ctacctgcag ccggctcttc
     1441 tcgtaattca cgctctccac cacgccgttg aaatcattga acggtccatc ggtgacccgc
     1501 accacctggc ccgcctcgaa cagcaccttc ggccgtggct tgtccacgcc ttcctcaacg
     1561 cgccgcagga tctggttggc ttccttctcg gagatcggcg ccggcttgtc gctggtacca
     1621 ccgatgaacc cgagcacctt gggcacgtcg cgcaccagat gccaggtctg ctcatccatt
     1681 tccatctgca ccagcacata gcccgggtag aacttgcgtt cactgcggcg cttctggccc
     1741 tcgcgcatct cgacgacttc ttccgtcggg acgaggatct cgccgaactt gtcctcaagc
     1801 ccgtcgcgct tgatccggtc cttcagggac tcggaaacgc ggtgctcgaa gttcgagtag
     1861 gcgtgcacca cataccatct catcgccatc
//
LOCUS       DASWPU010000253         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_32905,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000253 DASWPU010000000
VERSION     DASWPU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32905"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..308)
                     /locus_tag="VGC27_05450"
     tRNA            complement(219..308)
                     /locus_tag="VGC27_05450"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Ser,seq:tga)
     gene            437..1363
                     /locus_tag="VGC27_05455"
     CDS             437..1363
                     /locus_tag="VGC27_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY0282050.1"
                     /translation="MLRQTCAVIAFGALAAGCASAPEHRVSVPPNAGVYKVGNPYQIG
                     GTWYYPHEQPDYDETGIASWYGPGFYGRPTANGEIFDAESLTAAHRTLPLPVNVRVTN
                     LENGESTILRVNDRGPFAKGRIIDVSERAARLLGIYGPGTARVRVTYISRADLPDGRP
                     QPFGAGMPDAVVNAIPTVPTGKVETGTLDVIPGTEQAPPAAAPTLPAPAAAAPQTADL
                     GGLPTGKVTRVPVPAVTHLYVQVGAFSNYQNAARLAARLDGGLKISTIQRNGQTLYRV
                     RLGPFDTTDDADAALSRIISLGSNDAQIVVDQ"
     gene            1395..>2153
                     /locus_tag="VGC27_05460"
     CDS             1395..>2153
                     /locus_tag="VGC27_05460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY0282051.1"
                     /translation="MPRLRFFVLVSLLLALAPARAAMNTSAERALLMDAATGQVLWAK
                     DGFSPMPPASMSKLMTLELLFQRLKDGRVKLTDTFPVSERAWRTGGSKSFMKIGDNVP
                     VEALIRSIIIPSGNDACIVVAEALGGTTEGFIDLMNRRAKALGMKNSHFVNPHGLPDP
                     PGQMMSAYDLAVLARHLIDTYPAYYHYFSERQFTWTNITQSNHNKVLDKFPGADGLKT
                     GHTDAAGYGVTASAVRDGYRLILVLNGLRYTDLAK"
BASE COUNT          410 a          697 c          636 g          410 t
ORIGIN      
        1 aaatggtctc cggtccgtct ctttccgtct gtacttccga aagtggtctt agctgggcgc
       61 cgaatttcat ctgggatggt gatggcggcc tgcacgacaa tctgcaggct gtggtcgccg
      121 tcgcgaagat agcatccgct ctggaagggc gggggcgcga agtagcctgg agcaccaaag
      181 gacaaacatg cgcacaatgg tgcaaaatca gcggctggtg gcggatgggg tgggattcga
      241 acccacggta ccctttcggg cacgccggtt ttcaagaccg gtgccttcaa ccgctcggcc
      301 acccatccgg tgccggctca ttgccccgaa ccgcggccat aagcaaggct ttgccggccg
      361 ttcacggttg agccgcgaag cgccatcgtt tacccttcct cctcgtttta ggacttgatt
      421 ctgctagatg aatcgattgc tgcgacagac ctgcgccgtg atcgcgttcg gcgcgcttgc
      481 cgccggatgc gcttctgcgc cggagcacag ggtcagcgtt ccgcccaacg ccggtgtgta
      541 caaggtcggc aacccctatc agatcggcgg cacctggtac tatccgcacg aacagcccga
      601 ctatgacgaa accggcattg cctcctggta cgggccgggg ttctacggcc gtccgacggc
      661 aaatggagag attttcgacg ccgaatcgtt gacggcggcc caccgtacgt tgccgctgcc
      721 ggtgaatgtg cgcgtcacaa acctggaaaa cggagaatcc acaattctgc gcgtgaacga
      781 ccgcggacct tttgccaaag gccgtatcat cgacgtgtcg gaacgcgccg ccaggctgct
      841 cggcatttac ggacctggta ccgcgcgggt gcgcgttaca tatatttcac gcgccgacct
      901 tcccgacggc aggccgcaac ccttcggcgc tggcatgccc gacgccgttg tgaacgcgat
      961 tcccacagtg ccgaccggaa aagtcgaaac cggcacgctc gatgtaattc cggggacgga
     1021 acaggcgccc ccggccgccg cgcctacctt accggcacca gcggcagccg cgccgcagac
     1081 cgccgatctc ggcggcctgc cgacggggaa ggtgacccgc gttcccgtac cggccgtcac
     1141 tcatctctat gtccaggtgg gcgccttcag caattatcag aacgccgcgc gcctggcggc
     1201 gcgcctcgac ggtggattga agatttccac cattcaacgg aacggccaaa cgctctatag
     1261 agtccggcta ggcccgttcg atacgaccga cgatgccgac gcggcgttat ccaggatcat
     1321 cagcctcggc agcaacgacg cgcaaatcgt cgtcgaccaa tagcagtcag tttttttata
     1381 cttggagtgg ttccatgccg cgtttgcgct ttttcgttct cgtctcgctc ctcttggccc
     1441 tcgcgcccgc gcgcgccgcg atgaacacca gcgccgagcg tgcgcttctg atggacgcgg
     1501 ccaccggcca ggtattgtgg gcgaaggacg gcttttcgcc catgccgccc gcctccatga
     1561 gcaagctgat gacgctggaa ctgctgttcc agcgcctgaa ggacggccgc gtgaaactga
     1621 ccgacacatt cccggtatcc gaacgggcct ggcggacggg cggatcgaag tcattcatga
     1681 agatcggaga taatgttccg gtcgaagccc tcatccggag cattatcatc ccgtcgggca
     1741 acgatgcctg catcgtcgtt gccgaagcgt tgggcggcac gacggaaggg ttcatcgact
     1801 tgatgaaccg ccgcgccaag gcgcttggga tgaagaattc gcatttcgtc aacccccacg
     1861 gactccccga tcctcctggc cagatgatgt ccgcctatga tcttgcggtg ctggcacggc
     1921 acctgatcga cacttacccc gcttattatc actacttcag cgagcggcaa ttcacctgga
     1981 ccaacatcac ccagtcgaac cacaacaagg tgctcgacaa atttcccggc gccgacggcc
     2041 tcaagaccgg ccacaccgac gcggcgggct acggcgtgac ggcttcggca gtgcgagacg
     2101 gctatcgcct gatcctggtg ctgaacgggc tacgctatac cgatctggcc aag
//
LOCUS       DASWPU010000254         4413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_75472,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000254 DASWPU010000000
VERSION     DASWPU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75472"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /gene="nadA"
                     /locus_tag="VGC27_05465"
     CDS             <1..462
                     /gene="nadA"
                     /locus_tag="VGC27_05465"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY0282052.1"
                     /translation="ANQTAVKIIAWKGHCEVHERFTPADIRGFREASPGVVVLAHPEC
                     PPEVIAEADFAGSTSGMISYVGEHRPRRVVLVTECSMSDNVAVEYPDIAFLRPCNLCP
                     HMKRITLPKILKSLESLSVEVTVDAAVAARARASVERMLAVGNRRSGRTAA"
     gene            469..2052
                     /locus_tag="VGC27_05470"
     CDS             469..2052
                     /locus_tag="VGC27_05470"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY0282053.1"
                     /translation="MTLQADNVIETKGALILGAGAAGLFTALKLSPYPALVLADTHPG
                     VSGSSARAQGGIAASVGEGDTWQAHAADTVAAGAGLCDPAIAALVAREAAARIDYLVR
                     FGAPFDRRKNGSLAVGREAAHSAARIVHVNGDSAGAAVMRALTDSALATPSITLLEGF
                     HAIELAVEDGRVAGLFARYGVGGFSRLVLFRAQEVILATGGLGALYAVTTNPVETRGE
                     GLGMAARAGAVIADPEFVQFHPTAIDVGISPAPLATEALRGKGATLIDEAGRRFMPAI
                     HADAELGPRDVVARAIHRQIAAGHKVFLDCREAVGAKFPQAFPTVYAACHEAGIDPAT
                     QPIPVAPAAHYHMGGIATDTLGRTSLEGLRAVGECAATGLHGANRLASNSLLEALVFG
                     ARLADDLRGRGTLRASRGTLPKPEQFRALPPPRVLREAMSRLVGLERCADGLNEALRI
                     IAKIERAGAHEPTLLNMTATAKLVVAGALARHESRGSHFRTDYPQTDPVGVRTFLTLA
                     QAERMIAEPETQTATRSLA"
     gene            2049..2900
                     /gene="nadC"
                     /locus_tag="VGC27_05475"
     CDS             2049..2900
                     /gene="nadC"
                     /locus_tag="VGC27_05475"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY0282054.1"
                     /translation="MTTFSRPPHPLLLEPIVRRAVEEDLGRAGDVTSELVVPAGSRAK
                     ARLKTREPGRIAGLICAEAAFRLLDATLEFRLDARDGADAEAGSSLATLQGSARGILA
                     GERVALNFVGHLSGVATRTRALAAAISGTKARIVCTRKTTPGLRLLEKYAVRCGGGFN
                     HRFGLDDAVLIKDNHIVAAGGLATALERARAGAGHMMTIEVEVETLEQLEEALRLGVN
                     TILLDNMAMDDLKRAVAMTEGRAVLEASGNVTLATVRAIAETGVDYISSGAITHSAPC
                     LDVTLEF"
     gene            complement(3080..3733)
                     /locus_tag="VGC27_05480"
     CDS             complement(3080..3733)
                     /locus_tag="VGC27_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEY0282055.1"
                     /translation="MNYKSRDALLRSDRILVVAFAVVLAAVSAGVGAAAAYRPHTDQV
                     KTAVVVEPAAGVAEAAVPVAAQPADKSLVEELIVNKLKAEHRCLSEVLYYEARGEGVT
                     GQKAVAEVVFHRMRTGNYGHSICAVVYEGANKSGCQFSFACNGELKRAKAPRAWLRAQ
                     ILAARIFTGEESLGDVTGGATSFHAVSVQPDWANDLQRTVQIGNHIFYKGALRSRSM"
     gene            complement(3960..>4413)
                     /locus_tag="VGC27_05485"
     CDS             complement(3960..>4413)
                     /locus_tag="VGC27_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY0282056.1"
                     /translation="KERGKLPAYMIGTMIELPRAALRAAEIAETAEFFSFGTNDLTQT
                     TLGLSRDDAGMFLPDYLTKGILKTDPFVSIDRDGVGELIKIAAQRGRSTRAKLKLGIC
                     GEHGGDPDSIQLCHEAGLDYVSCSPFRVPVARLAAAQAALKTSIERDV"
BASE COUNT          761 a         1498 c         1407 g          747 t
ORIGIN      
        1 gccaatcaga ccgccgtgaa gatcatcgcc tggaaaggcc attgcgaagt gcacgagcgc
       61 ttcacgccgg ccgacattcg cggctttcgc gaagccagtc ccggcgtcgt cgttctcgcc
      121 catcccgaat gcccgcccga ggtgatcgcc gaagcggact tcgccggctc gacctccggc
      181 atgatctcct atgtcggcga gcaccgcccg cggcgcgtcg tgctcgtcac cgaatgttcg
      241 atgagcgaca acgtcgccgt cgaatatccc gatatcgcct tcctgcggcc gtgcaatctc
      301 tgcccgcaca tgaagcgcat cactttgccc aaaatcctga aatcgctgga aagcctgagc
      361 gtcgaggtga cggtcgatgc cgccgtcgcg gcgcgggcgc gcgcctcggt cgagcgcatg
      421 ctggcggtgg ggaaccggag gagcggccgc acggcggcct gaaacgccat gaccctgcaa
      481 gccgacaatg tcattgaaac caagggcgcc ttgatcctgg gcgcgggagc cgccggcctg
      541 ttcacggcgc tgaagctttc gccctacccc gcgctggttc tggccgatac gcatcccggc
      601 gtttcgggat cgagcgccag ggcgcaaggc ggcatcgccg cctccgtcgg cgaaggcgac
      661 acctggcagg cgcacgccgc cgatacggtc gccgccggcg cgggcctctg cgatcccgcg
      721 atcgcggctc tggtcgcgcg cgaggctgcc gcgcgcatcg actatctggt ccgtttcggc
      781 gcgcctttcg accggcgcaa gaacggctcg ctggcggtgg gccgcgaggc cgcgcattcc
      841 gccgcccgca tcgtccacgt caacggcgac agcgccggcg cggcggtgat gcgcgccttg
      901 accgacagcg cgctggcgac gccgtcgatc accttgctgg aaggctttca cgccatcgaa
      961 ctggcggtgg aggacggccg ggtcgcgggc ctgttcgcgc gctacggcgt gggcggtttc
     1021 tcgcggctgg tgctgttccg ggcgcaggaa gtgatcctgg cgacgggcgg gttgggcgcg
     1081 ctctacgccg tcaccaccaa tccggtcgaa acccgcggcg aaggcctggg catggcggcc
     1141 cgcgccgggg ccgtgatcgc cgatccggaa ttcgtgcagt tccaccccac ggcgatcgat
     1201 gtcggaattt ctcccgcgcc cttggcgaca gaagcgctgc gcggcaaagg cgccaccctc
     1261 atcgacgagg ccggccgccg tttcatgccg gcaatccatg ccgacgcgga actcggcccg
     1321 cgcgacgtcg tcgcccgcgc catccatcgc cagatcgccg ccgggcataa ggtgttcctc
     1381 gactgccgcg aagcggtggg cgccaagttc ccgcaggcgt ttccgacggt ctatgcggcc
     1441 tgccacgaag cgggcatcga tccggcgacg cagccgattc cggtcgcgcc cgccgcgcat
     1501 taccatatgg gcggcatcgc caccgacacg ctggggcgaa cctctctgga agggctgcgg
     1561 gccgtcggcg aatgcgcggc gacgggcctg cacggcgcca accggctggc ctccaactcg
     1621 ctgctcgaag cgttggtgtt cggcgcgcgc ctcgccgacg acctgcgcgg acgcggcacg
     1681 ctgcgcgcaa gccgcggcac gctgcctaag cccgaacaat tccgcgccct gccgccgccg
     1741 cgggtgctgc gcgaagcaat gagccgcctc gtcggactcg agcgctgcgc cgatgggctg
     1801 aacgaagcgc tgcgcatcat cgccaaaatc gaacgcgccg gcgcgcacga accgacgctt
     1861 ctcaacatga ccgccacggc gaaactggtg gtggcgggcg cactggcccg gcacgagagc
     1921 cgcggcagcc atttccgcac cgactatccg cagaccgatc ccgttggcgt gcgcaccttc
     1981 ctgacgctgg cgcaggccga acgcatgatc gcggaacccg agacccaaac ggcgacaagg
     2041 agtctggcgt gacgactttt tcccgccctc cccatccgct gcttctcgaa cccatcgtgc
     2101 gccgcgcggt ggaggaggat ctcgggcgcg ccggcgacgt caccagcgag ctggtggtgc
     2161 cggccggcag ccgggccaaa gcgcggctga agacgcgcga gcccggcagg atcgccggat
     2221 tgatctgcgc cgaagccgcc ttccgcctgc tggacgccac gctcgaattc cgtctcgatg
     2281 cgcgcgacgg cgccgatgcc gaagccggtt cgtccctggc gacgctgcaa ggatcggcgc
     2341 gcggcatcct ggcgggagaa cgcgtggcct tgaatttcgt cggccacctc tccggcgtcg
     2401 ccacccggac gcgtgccctg gccgccgcca tctccggcac caaggcgcgc atcgtctgta
     2461 cccgcaagac cacgcccggc ctgcgcctgc tggagaaata tgccgtgcgc tgcggcggcg
     2521 gcttcaatca tcgcttcggc ttggacgacg cggtgctcat caaggacaac cacatcgtgg
     2581 cggcgggcgg gcttgccacc gcgctggaac gcgcccgcgc cggcgccggc cacatgatga
     2641 cgatcgaagt cgaagtcgaa accctcgaac agctcgaaga agcgctgcgg ctcggcgtga
     2701 acacgattct gctcgacaat atggcgatgg acgatttgaa gcgcgccgtc gcgatgaccg
     2761 agggccgcgc cgtgctcgaa gcgtccggca acgtcaccct ggcgacggtt cgcgccattg
     2821 cggaaacggg cgtcgattac atttcatcgg gcgcgatcac gcactctgcg ccttgtctcg
     2881 acgtgacgct cgagttctag agcaaaatgc attcagacgg aatcgccccc acccttcggc
     2941 aagctcaggg tgagggcatt ttctaatcct tcgtgctggt cggcacgctg ttgttaccga
     3001 ctgtcgaagc atgaagacaa cgtgatctcg tctgaacgta cgaagctcta gcaatcacat
     3061 aatcacatcg agcacataat cacatcgagc gcgaacgaag cgcacctttg tagaagatgt
     3121 ggttgccgat ctgcaccgtg cgttgcagat cgttggccca gtcgggctgg accgagacgg
     3181 cgtgaaagct ggtggcgcct ccggtgacgt cgcccaggct ctcctcgccg gtgaagatgc
     3241 gcgccgccag gatttgcgcc ctcagccatg cccgcggcgc cttggcgcgc ttgagttctc
     3301 cgttgcaggc aaaggaaaac tgacaaccgg acttgttcgc gccttcatag accacggcgc
     3361 agattgagtg gccgtaattg ccggtgcgca tacggtggaa cacgacttcc gccaccgcct
     3421 tctggcccgt cacgccttcg ccgcgcgctt cgtaatacag cacttccgaa aggcagcgat
     3481 gctccgcctt gagcttgttg acgataagct cttcgaccag agacttgtcg gcgggctgcg
     3541 cggccacggg caccgcagcc tccgcgacgc cggccgcggg ttcgacgacc accgcggttt
     3601 tgacttggtc tgtatgcgga cggtatgcgg cggcggcccc tacgccggcg ctcacggccg
     3661 ccagcacgac cgcaaacgct acgaccagaa tacggtccga acgcaacaac gcgtctctgg
     3721 atttataatt catcgggaaa gactcccttc ggatttcgtt tcgtaaactt gcggcgttaa
     3781 ctccgaacag tccgttcctt ctccctcttg aaaggtcgcg ccttgtgacc aagaaaagcc
     3841 gaatcgtcaa atgaaaagtt ttgcaaacgt gacactacgt cacgcaacgt cacaaaaaaa
     3901 cagggaatcc ctggatattt tccagggatt cccgatttag tccgtgtact gattatgact
     3961 caaacgtcgc gttcgatcga cgttttgagc gccgcttgcg cagcggcaag ccgtgccacg
     4021 ggcacgcgga atggtgaaca cgacacgtaa tcgagtcccg cttcgtgaca aagctgtatc
     4081 gaatccggat cgccgccgtg ctcgccgcag atgccgagct taagcttggc gcgcgtcgag
     4141 cgcccgcgct gcgcggcgat cttgatcagt tcgccgacgc cgtcgcgatc gatcgaaacg
     4201 aagggatcgg tcttgagaat gcccttcgtt aaatagtccg gcaggaacat gccggcgtcg
     4261 tcgcggctca ggcctagcgt ggtctgggtc aggtcgttgg tgccgaacga gaagaactcc
     4321 gccgtttcgg cgatctccgc cgccctgagc gccgcgcggg gcaactcgat catggtgccg
     4381 atcatgtagg cgggaagctt gccgcgttcc ttc
//
LOCUS       DASWPU010000255         7853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_63483,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000255 DASWPU010000000
VERSION     DASWPU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63483"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VGC27_05490"
     CDS             complement(<1..222)
                     /locus_tag="VGC27_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282057.1"
                     /translation="MANGDGGNTSAPSGAAGRAPRIGMQRWVICALLFAATSLLYVDR
                     NTLGFLKHDLSVQYGWDQAEYAHIVQWFMV"
     gene            complement(319..1530)
                     /gene="manD"
                     /locus_tag="VGC27_05495"
     CDS             complement(319..1530)
                     /gene="manD"
                     /locus_tag="VGC27_05495"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF011654.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HEY0282058.1"
                     /translation="MLKIETAKVIVTCPARNFVTLKIETKDGVFGLGDATLNGRELAV
                     ASYLSDHVIPCLIGRDAHRIEDIWQYLYRGAYWRRGPVTMSAIAAVDMALWDIKAKVA
                     GLPLYQLLGGAARDGVMVYCHANGTSIEETIDAARAYMDQGFKAVRLQAGVPGLKSTY
                     GVPKGNKPYEPADADIPTENLWSTEKYMRSVPALFEAGRKHLGAEVHLLHDVHHRLTP
                     IEAGRLGKDLEPFRMFWLEDAVPAENQAGFRLIRQHTTTPLAVGEVFNSIWDCKQLIE
                     EQLIDYIRATVVHAGGITHLRRIAALADLYHVRTGCHGATDLSPVAMAAALHFDLSVP
                     NFGIQELMHHAAVSDAVFPHAYSYSDGYMHPGDQPGLGVDIDEKAASKYSYKRAYLPV
                     NRLEDGTLYNW"
     gene            1947..3332
                     /locus_tag="VGC27_05500"
     CDS             1947..3332
                     /locus_tag="VGC27_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0282059.1"
                     /translation="MTRTEETMTQKLPVTEKVGYALGDMAANFVFQFMIALQLDFYTH
                     TFGLSPAQAGTMLGVVGLSVAVLNPVMGVIADRTNTKWGKFRPWILWTALPFGIIGVL
                     TFTTPDMGIGAKIVYAWVTYTLLRVIYIANNVPYASLTAVMTDDPDERTSISSYRQIA
                     ANLAGLIIASFVIPLVGIFGQGNDARGYQLTMGLLCVVSVIFFAITFLVTKERIQPDP
                     QQKTDLGKDLKDLFQNRAWVILFLVTLFYFTAIVIRGNDMLPYFRFVASDVGLFSWFN
                     GFGLGALLLGVACSTAVSIRVGKLNLFIASMLLTGVFTTALLVIPPHATYVVIGTEVL
                     RQFSFGLSGPILWAMMGDVADYGEWKTGRRASGTVTAAVVFALWVGLALGGAIAGWLF
                     SFYGFVSDAVVQTPEAQSGILLTGSVYAGLAFFAAAALLFFYPLTSEKTKSMANELIE
                     RRKSFTGKSAS"
     gene            3437..5710
                     /locus_tag="VGC27_05505"
     CDS             3437..5710
                     /locus_tag="VGC27_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009044 - xylan 1,4-beta-xylosidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 52 protein"
                     /protein_id="HEY0282060.1"
                     /translation="MTKRRPLSCQIGFNAQHSPMGAFMSFTCGNPGTKGGIGLQIGQP
                     ADQEVFIGVIDGDRYADAPLKCLPFYIGAASTAAEAFTVEQAGPSEANVRPDAVPFAP
                     KEIQRFYGWATDTWQAGDLTFTVYTPFGSIPDPAKATKDEMREGLRPAVVAQLIVDNS
                     KGSKPKTGIFALNHSRPGPRMINDDLGSKKRVGFAFRREAGAAAEVFDVTDGKKGPAP
                     APFGYMRWSATDGIRERYNPVHLLGSCPGVGIEVPPGKKYAMTLAIGSYLEQPVTTGL
                     DGKYLYTRYYADLTDVLRSTLDRANKLMNAAEKLDKKLESSSLSYDQKFIIAHATHSY
                     YGSTQLLEIEKQPFWVVNEGEYCMMNTLDLAVDHVFWELDHNPWLVKNLLDSFVRHYS
                     YTDEIKIYKADSSGPVHSFDHDPSQPPPPPDAAQLSRPYDKKPGGLSFSHDMGAHNNF
                     APKGRSSYELANLTSTFSYMTVEQLCNWTLTAACYMAKTRDTGWLKQNKKVIEDCLTS
                     MVNRGGEVGFAQFDSTKTAGGQEITTYDSLDHSLAQTRNNVYMAVKSWASYRALALMF
                     RDLGDKKAQERCQQLAVKVAGVVAEQAVDGILPAIFEKSNPGYNSRILPAVEGCVYPL
                     YFAKTGYAGIKPAEIYATPAEQKMFDVLKEHTRKLLVDPQHRNTFDDGGIKLSSTSNN
                     SWMSKISIFMDVTRRVFGLDKDPQVAEVFKKADAAHVKWQTVGSAYWACSDQIVSGEG
                     KASRYYPRIITSALWLD"
     gene            5852..6934
                     /locus_tag="VGC27_05510"
     CDS             5852..6934
                     /locus_tag="VGC27_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEY0282061.1"
                     /translation="MAALTATASAQSPASLKEAYKDSFLIGVAINEAQIAGRDAISDA
                     IVKAQFNSISPEDALKWENIHPQPDRHDFAIPDKYVAFGQENHMFTVGHVLLWHNQTP
                     DWVFRDGKGNLLDREALLARLKHHIHTVVGRYKGKIKAWDVVNEAVDEDGSMRPSLWY
                     KIIGEDYVAEAFRYVHEADPEAELLYNDYSLENETKRAGAVALIKKLKAEGVPVTTVG
                     LQGHYVMDWPAPELLDAAISDFGKLGVKVAITELDIDVLPRPTGQQTAEVTLKIEQDP
                     ALNPYAGGLPDAVQKQLAQRYGEIFAVCVKHRDVVNRVTLWGVTDADTWHNNWPVKGR
                     TAYSLLFDRAGQPKPAYDAVIEAASR"
     gene            complement(6999..>7853)
                     /locus_tag="VGC27_05515"
     CDS             complement(6999..>7853)
                     /locus_tag="VGC27_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282062.1"
                     /translation="LRVTLPAHAKGPVPVIMELAFTGPWLATLPVPSGPDWRQQILAL
                     GWGYAELIPVSIQPDNGAGLTQGIIGLVNKGQPRKPDDWGALRAWAWGASRALDYFET
                     DPSVDATQIGIEGHSRYGKAALVAMAYDPRFAIAYISSSGAGGAKLLRRNFGETLENV
                     AGTGEYHWMAGNFLKYAGPLTAKDLPVDAHELIALCAPRPVFIGGGATQGDGWVDARG
                     MFMAAAAASPVYELLGKKGLNAAGLPPIETPLIAGDIAFRQHGGGHTPAPNWPAFLTF
                     AQRYIHLR"
BASE COUNT         1519 a         2520 c         2360 g         1454 t
ORIGIN      
        1 gaccatgaac cattgcacga tatgggcgta ctcggcctgg tcccaaccgt attggacgga
       61 cagatcgtgc ttcaggaagc cgagcgtatt gcggtccaca tagagaaggc tggtggcggc
      121 aaacagcagc gcgcaaatca cccagcgttg catgccgatg cgcggcgcgc gaccggccgc
      181 tccggagggg gcggaagtgt ttccgccgtc accatttgcc attttgctgc tctccctgaa
      241 tgcgcgatcc gtccaggccg ctttgcgctc tcgtaacgcg gccgttgtgg ttgccgtcgc
      301 ggcgttacac ttagattctt accagttgta caaggtgccg tcttccagcc ggttgaccgg
      361 aagataagcg cgtttatagg aatacttgga tgcggccttt tcgtcgatat cgacgccgag
      421 gcccggctgg tctcccggat gcatatagcc gtcgctgtag ctgtaggcgt gcggaaacac
      481 cgcgtcgctc acggccgcgt gatgcataag ctcctgaatg ccgaaattcg ggaccgaaag
      541 atcgaaatgc agcgcggccg ccatcgccac cggcgacaga tcggtggcgc cgtgacagcc
      601 ggtgcggacg tgatagaggt cggccaaggc ggcgatccgc cgcagatggg tgatgcctcc
      661 cgcatgcact accgtagcgc gaatgtagtc gatgagctgc tcttcgatca gctgcttgca
      721 gtcccagatc gaattgaaga cctcgccgac ggcgagcggc gtggtggtgt gctggcggat
      781 cagccggaac ccggcctggt tttccgccgg caccgcatcc tccagccaga acatgcggaa
      841 cggctccaga tccttgccca agcggcccgc ttcgatcggc gtcaggcggt gatgcacgtc
      901 gtgcagcaga tgcacctcgg ctccgaggtg cttgcgcccc gcttcgaaca gagcgggcac
      961 cgagcgcatg tatttttcgg tggaccaaag attctccgtg gggatgtcgg catcggccgg
     1021 ttcgtagggc ttgttgccct tgggaacgcc gtaggtcgat ttcaggccgg gaacgcccgc
     1081 ttgcagccgc accgccttga agccctggtc catataggcg cgcgccgcgt cgatcgtttc
     1141 ttcgatcgac gtaccgttgg cgtggcaata gaccatgacg ccgtcgcgcg ccgccccgcc
     1201 gagaagctga tacagcggca aacccgcgac cttggccttg atgtcccata gcgccatgtc
     1261 caccgcggcg atggcggaca tcgtgaccgg accgcggcgc caataggcgc cccggtagag
     1321 atactgccag atatcctcga tccgatgcgc atcgcgcccg atcaggcagg gaataacgtg
     1381 atcgctcaga tagctcgcca ccgccaactc gcgcccgttc agcgtcgcgt cgccgaggcc
     1441 gaagacaccg tccttggtct cgatcttgag ggtgacgaag ttccgcgccg gacaggtcac
     1501 gatgactttg gcggtttcga tcttcagcat cgggtcctca tttctccacg gtaagtacgg
     1561 tagttgtcag aacgaccgaa ttcggcctca ggatcatctc ggactcggcg acaaaggctc
     1621 tgcgcgtctg gcgctcccac agaatgaaac tatgttgcaa atctcccgca ccggcaaatg
     1681 ttgcagcgca agaaaatctg ccggtcccgc gccgagctct cggacaccgg ttttcggttg
     1741 ctctctggta ccgctaccac tacactggat caatagggca gcatccaggc tttcacaaca
     1801 aatgttgaat cgcgcgcctg cttcgcatcg cgttatgccg tcgctgcgct cacaatcctt
     1861 cttcgcagca tcggcacctt ttcgcacgac cccgattcgg caaaaccgat cccgcacata
     1921 aacaaatagc ccggcagaaa accggcatga cgagaacaga ggaaacgatg acccagaaac
     1981 tcccagtcac agagaaggtc ggctacgcgc tcggcgacat ggccgcgaac ttcgtcttcc
     2041 agttcatgat tgccctgcaa ctggacttct atacccacac cttcggcctc tcgccggctc
     2101 aggcgggaac catgttgggc gtcgtcgggt tgagtgtggc cgtcctgaat ccggtcatgg
     2161 gcgtgatcgc cgaccgcacc aacacgaaat ggggcaagtt ccggccgtgg attctgtgga
     2221 cggcgctgcc cttcggcatc atcggcgtcc tcaccttcac cacgcccgac atgggcatcg
     2281 gcgccaagat cgtctacgcc tgggtgacat acaccttgtt gagagtgatc tacatcgcca
     2341 acaacgtgcc ttacgcctcg ttgaccgccg tgatgacgga cgaccccgat gagcggacca
     2401 gtatctcgtc ctaccgccag atcgccgcga acctcgccgg cctgatcatc gccagcttcg
     2461 ttatcccgct ggttggcatc ttcggccagg gcaacgacgc gcgcggttac cagctcacca
     2521 tggggctgct ctgtgtcgtc agcgtcatct tcttcgccat caccttcctg gtgacgaagg
     2581 agcgcatcca gcccgacccg cagcagaaga ccgatctcgg caaggatctc aaggacctgt
     2641 tccagaaccg ggcctgggtc attttgttcc tggtgacgct gttctacttc actgccatcg
     2701 tgatccgcgg caacgacatg ctgccctact tcaggtttgt cgcctccgac gtggggctgt
     2761 tctcttggtt caacggattc ggccttggcg cgctgcttct gggtgtcgct tgctccaccg
     2821 ccgtgtccat ccgggtcggc aagctcaatc tcttcatcgc cagtatgctg ctgacgggcg
     2881 tcttcacgac ggcgctgctc gtgattccgc cgcacgccac ctatgtggtc atcggaacgg
     2941 aagtgctgcg ccagttctcg ttcggcctgt cgggcccgat cctctgggcg atgatgggtg
     3001 acgtcgccga ctacggcgaa tggaagaccg ggcggcgcgc cagcggcacg gtgacggccg
     3061 cagtggtgtt cgcgctctgg gtgggcttgg cgcttggcgg tgccatcgcc ggctggctgt
     3121 tctcgttcta cggtttcgta tccgacgccg tggtgcagac gccggaggcg cagtcgggca
     3181 ttcttctgac cggcagcgtt tacgccggcc tcgccttctt cgccgccgcg gcattgctct
     3241 tcttctaccc gctgacgagc gaaaaaacca agtcgatggc caacgaactg atagagaggc
     3301 gcaagtcctt taccgggaag agtgcctcgt aaggcggccc ggtaagactc cgcatcctgg
     3361 caagagacct ggagcatgcc aggctcttcg ccagggcctt tggaaagaac cgagatatca
     3421 agcacgagga aacgccatga cgaaacgccg cccgttatca tgccagatcg gcttcaatgc
     3481 ccagcactct cccatgggtg cgttcatgag cttcacgtgt gggaatccgg ggacgaaagg
     3541 cgggatcggc ctgcagatcg ggcaacctgc cgatcaggaa gtgttcatcg gcgtgatcga
     3601 cggcgaccgc tatgccgacg cccccctgaa atgcctgccc ttctatatcg gcgcggccag
     3661 caccgccgcc gaggctttca ccgtggaaca ggccgggccc tccgaggcga atgtgcgccc
     3721 ggatgcggtc cccttcgcgc ccaaggagat tcagcggttc tacggctggg ccaccgatac
     3781 ctggcaggcg ggcgatctca ctttcacggt ctatacgccc tttggttcga tccccgatcc
     3841 cgccaaggcc acgaaagacg aaatgcgcga agggctgcgc cccgccgtgg tggcgcaact
     3901 gatcgtcgac aacagcaagg gcagcaagcc caagaccggc atcttcgccc tcaaccattc
     3961 ccgtcccggc ccgcggatga tcaatgacga tctcggctca aagaagcgcg tgggcttcgc
     4021 cttccgccgc gaggcgggcg cggcggccga agtgttcgac gttaccgacg ggaagaaggg
     4081 accggcgccc gcgccgttcg gctacatgcg ctggtcggcc accgacggca ttcgcgagcg
     4141 ctacaatccg gtgcacttgc tcggctcgtg ccccggcgtg ggcattgagg tgccgccggg
     4201 caagaagtac gcgatgacgc tggcgatcgg ctcctacctg gaacagcccg tcaccacggg
     4261 cctggacggc aagtatctct acacccgcta ctacgccgac ttgaccgacg tgctgcgctc
     4321 gactctcgat cgcgccaaca agctgatgaa cgctgccgaa aagctcgaca agaagcttga
     4381 aagctcctct ctgtcctacg atcagaagtt catcatcgcg cacgccaccc acagctatta
     4441 cggttcgacg cagctcctcg agatcgagaa gcagcctttc tgggtggtga acgaaggcga
     4501 gtactgtatg atgaacacgc tcgacctcgc cgtcgaccac gtgttctggg aactcgacca
     4561 caatccctgg ctggtcaaga acctgctcga ctcgttcgtg cgccactaca gctacacgga
     4621 cgagatcaag atctacaaag ccgattcgag cgggccggtg cacagtttcg atcacgatcc
     4681 gtcgcagcct ccgccgccgc ccgatgcggc gcagctctcc cggccttacg acaagaagcc
     4741 cgggggcctc agcttcagtc acgacatggg cgcgcacaac aacttcgcgc ccaagggccg
     4801 ttcgagctac gagctggcaa acctcaccag caccttcagc tacatgacgg tggaacagct
     4861 ctgcaattgg actttgaccg ccgcttgcta catggcgaaa acgcgcgata cgggctggct
     4921 gaagcagaac aagaaggtca tcgaggactg cctgaccagc atggtcaacc gcggcggcga
     4981 ggtcggcttt gcccagttcg acagcaccaa gaccgcgggc ggccaggaga tcaccaccta
     5041 cgactccctc gaccactcgc tggcgcaaac gcgcaacaac gtctacatgg cggtcaagag
     5101 ctgggcgagc tatcgcgccc tggccctgat gttccgcgat ctcggcgaca agaaggcgca
     5161 agaacgctgc cagcagcttg ccgtgaaggt tgccggcgtg gttgccgagc aagcggtgga
     5221 cggtatcctt cccgccatct tcgagaaaag caatcccggc tacaattccc gcatccttcc
     5281 ggcggtggaa ggctgcgtgt atccgctcta cttcgccaag accggctatg ccggcatcaa
     5341 gcccgcagag atctacgcca ccccggccga gcagaagatg ttcgacgtgc tcaaggaaca
     5401 cacccgcaaa ctgctggtcg atccccagca ccgcaacacc ttcgacgacg gcggcatcaa
     5461 gctttcctcc accagcaaca attcgtggat gagcaagatc tcgatcttca tggatgtgac
     5521 gcgccgcgtc ttcggtctcg acaaagaccc gcaggtggcc gaggtgttca agaaggccga
     5581 tgccgcgcat gtgaaatggc agacggtggg cagcgcctat tgggcctgca gcgaccagat
     5641 cgtgtccggc gaaggcaagg cgagccgtta ctatccgcgc atcatcacca gcgcgctctg
     5701 gctggattga gttcgcgctg actttcgctt gcggcggaaa ggcggtctca atccggagcg
     5761 ccgcctttcc gttttggaac agaggacgag acttttcccg aaggaggaac gactcatgca
     5821 ggcacgatcg gcagccttga cggcaattct catggccgct ctgacggcca cggcatcggc
     5881 ccagtcaccc gcctcgctga aagaggccta taaggacagc tttctcatag gagtcgccat
     5941 caacgaggct cagatcgccg gccgggacgc aatcagcgac gctatcgtca aagcgcagtt
     6001 caattccatc tcgccggaag acgctttgaa gtgggagaac atccatcctc agccggatag
     6061 acacgacttc gcgatcccgg acaaatatgt ggcctttggc caggagaacc acatgtttac
     6121 cgtcggccac gttttgctgt ggcacaacca gactccggat tgggtgttcc gcgacggcaa
     6181 aggcaatctg ctcgaccgcg aggccctgct cgcgcgcctc aagcatcaca tccacaccgt
     6241 cgtcggccgc tacaaaggca agatcaaagc ctgggacgtg gtcaacgagg cggtggatga
     6301 agacggcagc atgcgtccgt cgctgtggta taagatcatc ggcgaggatt acgtcgccga
     6361 ggctttccgt tatgtgcacg aggccgatcc ggaggccgaa ctgctctaca acgactacag
     6421 tctcgagaac gaaactaagc gtgcgggcgc ggttgcgctc atcaagaagc tcaaggctga
     6481 gggcgtgccg gtcacgaccg tggggctcca gggccactac gtcatggact ggccggcgcc
     6541 ggagttgctg gacgccgcca tctccgattt cggaaagctg ggcgtgaagg tcgccatcac
     6601 cgaactcgac atcgacgtcc tgcctcgccc cacgggccag cagaccgccg aggtgacgct
     6661 caagatcgag caggacccgg cgttgaatcc ttacgctggc ggcctgcccg atgccgtgca
     6721 gaagcagctc gcgcaacgct acggcgagat cttcgctgtg tgcgtgaagc accgtgacgt
     6781 cgtcaaccgg gtgacgctgt ggggcgtgac cgacgccgat acctggcata ataactggcc
     6841 ggtcaaggga cgcaccgcct actcgcttct cttcgaccgt gccggacaac ccaagccggc
     6901 ctatgatgcg gtgatcgaag cggcaagcag atagcgccgc gcccgcattg ccgcgcggcg
     6961 gtgtgcaatt gatcgtgaac ggacggtttg gtccggcctt atcgcaaatg gatatagcgt
     7021 tgagcgaaag tgagaaacgc gggccagttg ggagccggtg tgtgtccgcc gccatgctgg
     7081 cggaaagcga tgtcgccggc gatcagcggc gtttcgatcg gcggcaagcc ggcggcgttc
     7141 aaacccttct ttcccaggag ttcatagacc gggcttgcgg ccgccgccgc catgaacatg
     7201 ccgcgcgcat cgacccagcc gtcgccttgc gtcgctccgc cgccgatgaa caccggacgc
     7261 ggcgcgcaca gcgcgatcag ttcgtgcgca tccaccggca gatccttcgc cgtcaacggg
     7321 cccgcatatt tcaggaagtt gcccgccatc cagtggtact cgcccgttcc cgcgacgttt
     7381 tccaaggtct cgccgaaatt ccggcgcaac agcttggctc cgcccgcgcc ggacgaactg
     7441 atgtaggcga tggcgaaacg cggatcgtag gccatggcga ccagagccgc cttgccgtag
     7501 cgtgaatgtc cttcgatgcc gatctgcgtt gcgtccacgg agggatcggt ctcgaaataa
     7561 tcgagcgcgc ggctggcgcc ccatgcccag gcgcgcagcg cgccccagtc gtccggtttg
     7621 cgcggctgtc ctttgttgac caggccgatg atgccttgcg tcagcccggc gccattgtcg
     7681 ggctggatac tgacgggaat aagttcggca tagccccagc ccagcgcgag aatctgctgg
     7741 cgccaatcgg gaccggacgg aacgggtagc gtcgccagcc atggcccggt gaaagccaat
     7801 tccatgatca ccggcaccgg gcccttggca tgggccggga gcgtcaccct cag
//
LOCUS       DASWPU010000256         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_112519,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000256 DASWPU010000000
VERSION     DASWPU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112519"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VGC27_05520"
     CDS             complement(<1..1427)
                     /locus_tag="VGC27_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0282063.1"
                     /translation="MFVAAGAAPFAAAAPSLADEAQPQFAPGVLSQDDVRLYRQIIAA
                     ERAGKFGLSQALYEKLGDTSLKGYIQAEHMLSPHSGRTPVSQMTAWLRSYGDLPIAAR
                     VHKLAVKRATKKVRKRHHKVVTVMTASIPAPTAARPRGGGYEDSDAPDPPIVSDAARA
                     VLEQISADIRADDPDQAYATLQALIAANTAPASDISRLSQRVCYSYVAEGMDDKAFAL
                     GDAAAATGRTDAPLLDWCAGISAFRLGKFEDAARHFEILSQVSSMANWTRGAAAFWAA
                     RSYLRAGDPSRVVPLLTLAANAKPTFYGLLAERLLGEDPQTGFADPVLLPGDFDRIMQ
                     SLPAHRAAALWQIYMKGYDYFVNTELNRAFGEAADLKLDAAFAALARQLDVPNLELRA
                     SETSASRGLLLTGLFPIPQYKPLGGYTIDPSLVLAFVRIETRFQARAISPAGAKGLMQ
                     LMPITAAHVGGKGAYDGLFDPGYNMS"
     gene            complement(1481..>2011)
                     /locus_tag="VGC27_05525"
     CDS             complement(1481..>2011)
                     /locus_tag="VGC27_05525"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0282064.1"
                     /translation="TATHTVFADGNPASRIMFIGEAPGRDEDRIGLPFVGRAGKLLDK
                     MMAAIHLDRSNAYITNVLNWRPPDNRDPSPEEAAMCLPFLRRHIELVNPGIIILLGAV
                     AARHVMGFSEGIMKLRGRWLDYRVGDGMVPVLPTLHPAYLLRQPAHKKLAWRDLQAAE
                     QKIVSLGLLEDRAAVR"
BASE COUNT          367 a          619 c          693 g          332 t
ORIGIN      
        1 gacatgttgt agccgggatc gaacaagccg tcataggcgc ccttgccgcc gacatgcgcc
       61 gccgtgatgg gcatcagctg catcaggccc ttcgccccgg cgggcgagat cgcgcgcgcc
      121 tggaagcggg tttcgatgcg cacgaacgcc agcaccagcg acggatcgat ggtgtagccg
      181 cccagcggct tgtattgcgg aatgggaaac aggccggtga gcaaaaggcc gcgcgaggcg
      241 ctggtttcgc tggcgcgcaa ttcgaggttg ggaacgtcga gctggcgcgc cagcgcggcg
      301 aacgcggcgt ctagcttgag gtcggcggct tcgccgaagg cgcggttcaa ctccgtattg
      361 acgaaatagt cgtaaccttt catgtagatc tgccacagcg ccgccgcgcg atgcgcgggc
      421 agactctgca tgatacggtc gaaatcgccg ggcagcagca ccggatcggc gaagccggtc
      481 tgcggatcct cgcccaacag gcgttcggcc agaaggccgt agaaggtcgg tttggcgttc
      541 gcggccaagg tgaggagggg aacgacgcgg ctcggatcgc cggcgcgcag gtaggaacgc
      601 gcggcccaga aggcggcggc gccgcgcgtc caattcgcca tcgaggagac ttgcgacagg
      661 atttcgaaat gccgcgccgc gtcttcgaat ttacccagcc ggaaggcgga gatccccgcg
      721 caccagtcga gcagcggcgc gtccgtgcgc ccggttgcgg cggcagcgtc gcccagcgcg
      781 aaggctttgt cgtccatccc ttcggcgaca taggagtagc agacgcgctg cgacaggcgc
      841 gaaatatcgg acgcgggcgc ggtgttcgcc gcgatcaatg cctgaagcgt ggcataggcc
      901 tggtcgggat cgtcggcgcg gatgtcggcg gagatctgtt ccagcaccgc gcgcgcggcg
      961 tccgagacga tcggcggatc gggcgcatcg ctgtcttcat agccgccgcc gcgcggacgc
     1021 gccgcggtcg gcgccggaat cgaggccgtc atcaccgtga cgaccttgtg atggcgcttg
     1081 cggactttct tggtcgcgcg cttgaccgcg agcttgtgga cgcgcgcggc gatgggcaga
     1141 tcgccatagc tgcgcagcca cgccgtcatc tgggaaaccg gcgtgcgccc ggaatgcggc
     1201 gaaagcatat gctcggcttg gatgtagccc ttcagcgacg tgtcgccgag cttctcgtaa
     1261 agcgcctggg acaagccgaa ttttccggcg cgttccgcgg cgatgatctg gcggtaaagc
     1321 cgcacgtcgt cctgcgacag cacgccgggc gcgaattgcg gctgggcttc gtcggccagc
     1381 gacggcgcgg ccgctgcgaa cggcgcggct cccgcagcaa caaacataaa cgcgaacgcg
     1441 aaacggcgta tcatatccgg cctttcgagg tcgcagagtg ttagcggacc gcggcccgat
     1501 cctcaagcaa gcctagggac acgatttttt gttccgccgc ttgaagatcg cgccaagcca
     1561 gttttttgtg cgcgggctgg cgcaaaagat aggccggatg cagcgtgggc agcacgggaa
     1621 ccataccatc tccgacacga tagtccagcc agcgtccgcg cagcttcata attccttcag
     1681 aaaatcccat cacatggcgc gcggccaccg cgcccaacag gatgataatt cccggattca
     1741 ccagttcgat gtggcggcgc aggaagggca ggcacatggc cgcttcctcg ggcgacggat
     1801 cacgattgtc gggcgggcgc cagttcaaaa cgtttgtaat gtaagcattc gaacgatcca
     1861 gatggatcgc cgccatcatc ttatcgagaa gcttgccggc gcggccgacg aagggcaggc
     1921 cgatgcggtc ttcgtcgcgg cccggcgctt cgccgatgaa catgatgcgc gaggccggat
     1981 tgccgtcggc aaagacggtg tgggtcgccg t
//
LOCUS       DASWPU010000257         7301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_26380,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000257 DASWPU010000000
VERSION     DASWPU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26380"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGC27_05530"
     CDS             complement(<1..188)
                     /locus_tag="VGC27_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282065.1"
                     /translation="MSDFGVPGGTFSTPPDAPPAEGELGNLARPSSALALLVVFRHRN
                     YRLFFSGQIVSLMGTWMQT"
     gene            328..846
                     /locus_tag="VGC27_05535"
     CDS             328..846
                     /locus_tag="VGC27_05535"
                     /inference="COORDINATES: protein motif:HMM:NF016286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEY0282066.1"
                     /translation="MAKDYIGYQALTDAALRGVVRDALRRIEKQGLIGSHHFYLTFKT
                     HFPGVEIPGFLREQYPEEMTIILQHQFWGLKVKDEQFEVALTFRKLPATLVIPFAALT
                     AFFDPGVQFGLQFKGMEGEAKAGGPAQVSPPQPAAEQNHTEPEEPAPAVEAPEKPAAP
                     AEVVSLDSFRKK"
     gene            962..2353
                     /gene="fumC"
                     /locus_tag="VGC27_05540"
     CDS             962..2353
                     /gene="fumC"
                     /locus_tag="VGC27_05540"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005107031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY0282067.1"
                     /translation="MIKTRTETDMFGPIEVPADRIWGAQTQRSLENFKIGWEKQPAPI
                     VRALGIVKRASAETNMDLGKLDPKLGQAIVAAAQEVIEGKLDAHFPLAVWQTGSGTQS
                     NMNANEVISNRAIEMLGGEMGSKQPVHPNDHVNMSQSSNDTFPTAMHIACTEEIVHRL
                     IPALQKLRNALNDKAQAWKRIVKIGRTHTQDATPLTLGQEFSGYTQQVENGIARIEQT
                     LPKLMQLAQGGTAVGTGLNAPVGFAEAVADKIAAITGLKFTSAPNKFEALAAHDAMVF
                     SHGAINTVAASLFKIANDIRFLGSGPRSGLGELALPENEPGSSIMPGKVNPTQCEALT
                     QVCVQIFGNHAAITFAGSQGHFELNVFNPVMAYNFLQSVRLMADAAASFSDHCVAGIE
                     PRLDNIKAGLERSLMLVTALAPKIGYDAAAKIAKTAHKNGTTLKEEAVKSGLVTAEEF
                     EALVRPEEMIGPK"
     gene            2453..3190
                     /locus_tag="VGC27_05545"
     CDS             2453..3190
                     /locus_tag="VGC27_05545"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY0282068.1"
                     /translation="MPRKRRTPASPHDDGFVIEQNNSTGGISFWERTDNQSVADRRGI
                     SLVDYIHAMYFFLRQAKCRHVLMIGCGGGTLATMLRHVGVKVTIADIDGRNFEIARTY
                     FHMPDDVECHVADGRDFLRRDPRRYDAIVLDAYSDNAMPKHFLKRAFFELVKSRLRPR
                     AAMVLVNLMAAGDDDRGPDRFARLMRKTWSQVRLLDGKCGESRNAVAVAGCVRKLKRP
                     RLVLRPQRCAKKMAAELRAMDFRPLRA"
     gene            3200..3421
                     /locus_tag="VGC27_05550"
     CDS             3200..3421
                     /locus_tag="VGC27_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY0282069.1"
                     /translation="MALKKRSFSLSRHRTSVALEPEFWAVLEGEAQRAGKSLAAFVAE
                     IDARRGMRPLASALRLTALAAAQASTHLR"
     gene            3508..3690
                     /locus_tag="VGC27_05555"
     CDS             3508..3690
                     /locus_tag="VGC27_05555"
                     /inference="COORDINATES: protein motif:HMM:NF025143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HEY0282070.1"
                     /translation="MAEIVNLRRVRKDKARKAKDVVAKANRAEHGVSKSAHKLAKTRA
                     KIEARDVDAHKLDKDD"
     gene            3735..6050
                     /locus_tag="VGC27_05560"
     CDS             3735..6050
                     /locus_tag="VGC27_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0282071.1"
                     /translation="MSGRYHDPLDEAFGSEPAVPAVPHEPAYLAGLNKEQREAVEATE
                     GAVLVLAGAGTGKTRVLTTRLAHILATRRAWPGQILAVTFTNKAAREMKDRIGALIGG
                     VVEGMQWLGTFHSIGARMLRRHAELAGLKSNFTILDADDQLRLMKQLIEAENIDEKRW
                     PARTLAAMIDGWKNRGQRPEDVPDGDAHSYAFGKGRKLYADYQARLKALNAADFGDLL
                     LETLTLLRGHPDILADYRERFRYILVDEYQDTNVVQYLWLKLLAGTGGNVCVVGDDDQ
                     SIYGWRGAEIENILRFERDFPGAKVIRLERNYRSTPAILGAASGLIAANKGRLGKTLW
                     TEGDPGEKIQVKGVWDAEEEARGVASDAEALHREGHLFAQMAILVRASFQMREFEDRF
                     IALGLPYRVIGGPRFYERAEIRDAMAYLRLVAQGDDDLAFERIVNKPKRGIGDASVQA
                     LHQFARVRQVPLLAAAREIVETDELPPKAKKSLGDLARNFARWADTARVLPHTELAEM
                     ILDESGYTDMLKADKSADAPGRLDNLKELVRSMEQFESLAAFLEHVSLVMELEQNETG
                     DRLNLMTLHAAKGLEFDTIFLPGWEEGLFPSQRTMDENGLAGLEEERRLAYVGLTRAK
                     QRALISFAANRRVHGSWQSALPSRFISELPEEHVETTMDEGFYGGNVGFRDNAFGKEF
                     ASTYDSPGWKRAQANKNQGYQRARPPLIEAQTFTVQTSDPSATHFAKGERVFHQKFGY
                     GLVKTVEGNKLTVDFDKAGEKKVIDQFVTRG"
     gene            6211..7095
                     /locus_tag="VGC27_05565"
     CDS             6211..7095
                     /locus_tag="VGC27_05565"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0282072.1"
                     /translation="MITPPLWKASVTLTKAEAHDVAAAFELTGPQAVLIAEEPFTDEA
                     EVEALYGVMPDGDLLSRLAGHEVRVIILPDADWIKLSQEGLPPVRAGRFFVYGAHDAG
                     KVPPGVIPMRIEAGMAFGTGHHETTALCLAVLSDLAKRRRFAKVLDLGCGTGLLAIGA
                     AKLWRRRVLASDIDPAAVEITRENAAANGEAPFVRAVAADGLTHPAIAESAPFDLIVA
                     NILAGPLTLLAPAIVRALAPGGMLVLSGLLQWQENLVAGYYRPLGLILRRKRRDGAWS
                     ALVLERPNAPFDKLRVKD"
BASE COUNT         1446 a         2250 c         2404 g         1201 t
ORIGIN      
        1 gtctgcatcc aggtgcccat cagcgagacg atctggccgg aaaagaaaag ccggtagttg
       61 cggtggcgga aaaccacaag aagcgcgagc gccgacgacg gcctggcgag attgcccaat
      121 tccccttcgg ctggaggcgc atcgggcggc gtggagaagg tcccgcccgg aaccccgaaa
      181 tcgctcacga ctcaggcgcc atatcaaaat cgttcgtcat cattgcgacg gaggtactct
      241 ttgcgccgtt ccggctcaag aggcgggcgc cgcattgact cgtcacgtcg ccggtttagt
      301 ctcaggcgca tatactcgga tgagcgcatg gcgaaggatt acatcggtta tcaagccctg
      361 acggacgcgg cgctgcgcgg cgtcgtccgc gacgctttgc gccgcatcga aaagcagggc
      421 ctgatcggct cgcatcactt ctatctcact ttcaagaccc attttcccgg cgtcgagata
      481 cccggcttcc tgcgcgagca atatccagaa gaaatgacca tcatcctcca gcaccagttc
      541 tggggcctga aggtgaagga cgaacagttc gaagtggcgc tgaccttcag gaaacttccg
      601 gcgacgctcg ttattccttt cgcggcgctg acggcgtttt tcgatcccgg cgtgcagttc
      661 gggctccagt tcaagggcat ggaaggcgaa gccaaggccg gcggcccggc gcaggtgtcg
      721 ccgcctcagc ccgcagccga gcagaatcac accgagcccg aggaacccgc gccggccgtc
      781 gaagccccgg aaaagcccgc cgcaccggcc gaggtggtga gcttggattc gttccggaag
      841 aaataagggc acccttcgac aagctcaggg tgagggctta aacatctcct tcatgctgag
      901 ctcgtcgaag catgagctcg tcgaagcatg agttcgtcga agcgtgaaag accgaattgc
      961 catgatcaag acccgcaccg aaaccgatat gttcggaccc attgaagttc ccgccgaccg
     1021 catttggggc gcgcaaacgc agcgatcgct ggagaatttc aagatcggct gggagaagca
     1081 gcccgccccc atcgtgcgcg cgctcggcat cgtcaagcgc gcgagcgccg agaccaacat
     1141 ggatctgggc aagctcgatc ccaaactggg acaggccatc gtggcggcgg cgcaggaggt
     1201 gatcgagggc aagctcgacg cgcatttccc gctggcggta tggcagaccg gctccggcac
     1261 gcaatccaac atgaacgcca acgaggtgat ttccaaccgc gcgatcgaga tgctgggcgg
     1321 cgagatggga tcgaaacagc cggttcatcc caacgatcac gtcaatatga gccagtcgtc
     1381 gaacgatact tttcccaccg cgatgcatat cgcctgcact gaagagatcg ttcaccggct
     1441 gatcccggcg ctgcaaaagc tgaggaatgc gctcaacgac aaggcgcagg cgtggaaacg
     1501 catcgtcaag atcggccgca cccatacgca agacgccacg ccgctcaccc tcggccagga
     1561 attctccggc tatacgcagc aggtcgagaa cggtatcgcg cgcatcgaac agacgctgcc
     1621 gaagctgatg caattggcgc aaggcggaac cgcggtgggc accgggctga acgcgccggt
     1681 gggattcgcc gaagccgtcg ccgacaagat cgcggcgatc accggcttga aattcacctc
     1741 cgcgcccaac aagttcgagg cgctggccgc ccacgacgcg atggtgtttt cccacggcgc
     1801 gatcaacacc gttgcggcct ccctgttcaa gatcgccaac gacatccgct ttctgggatc
     1861 ggggccgcgt tcgggcctgg gcgagctggc gctgccggaa aacgagccgg gctcctcgat
     1921 catgccgggc aaggtcaacc ccacccagtg cgaagcgctg acccaggttt gcgtgcagat
     1981 cttcggcaac catgcggcga tcaccttcgc gggcagccag ggccatttcg aactcaacgt
     2041 cttcaacccg gtgatggcct ataatttcct gcaaagcgtg cggctgatgg ccgacgcggc
     2101 ggcgagcttc agcgaccatt gcgtggcggg gatcgagccg cgcctggaca acatcaaggc
     2161 cggactggaa cgctcgctga tgctggtgac ggcgctggcg cccaagatcg gttacgacgc
     2221 ggcggccaag atcgccaaga cggcgcacaa gaacggcacc acgctgaagg aagaagccgt
     2281 caagagcggc ctggtgacgg cggaagaatt cgaggcgctg gtcaggccgg aagagatgat
     2341 cgggccgaaa taggctttta ccctccctca aggggaaggt tgaagtctgg ttattatgct
     2401 gaaaacgcgc tctatttccc tttcccgcca tcgcaccagt gtcacgcaag atatgccccg
     2461 caaacgccgc acgcccgcaa gccctcatga cgacggtttc gtcatcgagc agaacaactc
     2521 gaccggcgga atctcctttt gggaacggac cgacaatcag agcgtcgccg accggcgcgg
     2581 cataagcctg gtcgattaca tccatgcgat gtacttcttc ctgcggcagg caaaatgccg
     2641 tcacgtgctt atgatcggct gcggcggcgg cacgctggcg accatgctgc ggcacgtcgg
     2701 cgtcaaggtg acgatcgcgg atatcgacgg gcgcaatttc gagatcgcgc gcacctattt
     2761 tcacatgccc gacgacgtcg aatgccatgt cgccgacggg cgggatttcc tgcggcgcga
     2821 tccaaggcga tacgacgcca tcgtgctgga cgcctatagc gacaacgcca tgccgaagca
     2881 tttcctgaag cgcgccttct tcgaactcgt gaagtcgcgg ctcaggccgc gcgcggcgat
     2941 ggtgctggtc aacctcatgg cggccggcga cgacgaccgc ggcccggatc gctttgcgcg
     3001 gctgatgcgg aaaacctggt ctcaggtgcg gctgctggac ggcaaatgcg gcgaaagccg
     3061 caacgccgtc gccgtggcag ggtgcgtgcg caagctcaag cggccgagac tggttttgcg
     3121 cccgcaacgc tgcgccaaga agatggcggc cgaactccgg gcaatggatt tcaggccgtt
     3181 acgagcgtag agcgaaagga tggcccttaa gaagcgctcc ttctctctct cccgccatcg
     3241 caccagcgtc gcgctggagc cggaattctg ggcggtgctg gaaggcgaag cgcaacgggc
     3301 gggcaagagc cttgccgcgt tcgtggcgga gatcgacgcc aggcgcggga tgcgcccgct
     3361 ggccagtgcg ctgcgcctca ccgcgcttgc ggcggcccaa gcttcgaccc acctacgcta
     3421 aagcttcggc ggggtaggtt atagaattgg ggccgccgta gcatcgcgga ggtggtcaag
     3481 ctcggcatga gggggtaagc tcgttccgtg gcagagatcg taaacctgcg ccgggtgcgc
     3541 aaagacaagg cccgcaaggc gaaagacgtg gtggccaagg ccaatcgcgc cgagcatggc
     3601 gtctcgaaaa gcgcgcacaa gctggcgaaa acgcgcgcga aaatcgaggc acgcgacgtt
     3661 gacgcgcaca agctggataa ggacgattga ggtccctaca atagcctcat gttccacgac
     3721 tcgcgtgttt tcccttgagc gggcgctatc acgatccgct ggatgaggcg ttcggcagcg
     3781 aaccggccgt gccggcggtg ccgcacgaac cggcttatct tgccgggctg aacaaggaac
     3841 agcgcgaggc ggtggaagcg accgaaggcg cggtcttggt gctggcgggc gcgggcaccg
     3901 gcaagacgcg ggtgctgacc acgcggctgg cgcatattct cgccacgcgg cgcgcctggc
     3961 cgggacagat tctcgccgtc accttcacca acaaggccgc gcgcgaaatg aaggaccgca
     4021 tcggcgcctt gatcggcggc gtggtcgaag gcatgcaatg gctgggaacc ttccactcca
     4081 tcggcgcacg gatgctgcgc cgccacgccg aactggccgg gctgaaatcc aatttcacca
     4141 tcctcgacgc cgacgaccag cttcgcctga tgaagcagtt gatcgaggcc gaaaacatcg
     4201 acgagaaacg ttggccggcg cggacgctgg cggcgatgat cgacggctgg aagaaccggg
     4261 gccagcggcc ggaagacgtg cccgacggcg acgcgcactc ctacgccttc ggcaaggggc
     4321 gcaaacttta cgccgattac caagcgcgac tgaaggcgct gaacgcggcg gatttcggcg
     4381 acctgctgct cgaaacgctg acgctgctgc gcggccatcc ggacatcctg gccgattacc
     4441 gcgagcgctt ccgctacata ttggtggacg aatatcagga taccaacgtc gtccaatatc
     4501 tgtggctgaa gctgttggcg ggaaccggcg gcaatgtctg cgtggtgggc gacgacgatc
     4561 aatccatcta tggctggcgc ggcgcggaga tcgaaaacat cctgcgcttc gagcgcgatt
     4621 ttcccggcgc gaaagtgatc cggctggagc gcaattaccg ctcgacgccc gcgatcctgg
     4681 gcgcggcctc gggcctgatc gcggcgaaca aaggccgcct cggcaagacc ctgtggaccg
     4741 aaggcgatcc cggcgagaag atccaggtga agggcgtgtg ggacgccgaa gaagaagcgc
     4801 gcggggtggc gtccgatgcc gaagcgctgc atcgtgaggg gcatctgttc gcccagatgg
     4861 cgatcctggt gcgcgccagc ttccagatgc gcgaattcga agaccgcttc atcgcgctcg
     4921 gcctgcctta ccgggtgatc ggcggaccgc gcttctacga acgcgccgaa atccgcgacg
     4981 cgatggcgta tctgcgcctc gtggcgcagg gcgacgacga cctcgccttc gagcgcatcg
     5041 tcaacaagcc caagcgcggc atcggcgacg ccagcgtgca ggcgctgcac cagttcgcgc
     5101 gggtgcggca ggtgccgctc ttggcggcgg cgcgcgaaat cgtggaaacc gacgagctgc
     5161 cgcccaaggc gaagaaatcg ctgggcgatc tggcgcggaa tttcgcgcgc tgggccgata
     5221 cggcgcgggt gctgccgcac accgaactgg ccgaaatgat cctggacgaa tccggctata
     5281 ccgacatgct gaaggccgac aaatcggcgg acgcgccggg acggctggac aacctcaagg
     5341 aactggtgcg ctcgatggag cagttcgagt cgctggcggc gttcctggag catgtctcgc
     5401 tggtgatgga gctggagcag aacgaaaccg gcgaccggct gaacctgatg acgctgcacg
     5461 cggccaaggg gctggagttc gacaccatct tcctgcccgg ctgggaggaa gggctgtttc
     5521 ccagccagcg caccatggac gagaacggct tggcgggatt ggaagaggag cgccggctgg
     5581 cttatgtcgg cctgacccgc gccaagcagc gggcgctgat ttccttcgcc gccaaccggc
     5641 gggtgcatgg aagctggcaa agcgcgctgc cctcgcggtt catttccgaa ttgcccgaag
     5701 agcatgtcga aaccaccatg gacgaaggct tttacggcgg caatgttggc ttccgcgaca
     5761 atgccttcgg caaggaattc gcctcgacct acgacagccc cggctggaaa cgggcgcaag
     5821 cgaacaagaa ccagggctat cagcgcgccc gtccgccgct gatcgaggcg cagacgttca
     5881 cggtgcagac gtccgatccc tcggccacgc atttcgcgaa gggcgaacgc gtgttccatc
     5941 agaaattcgg ctatggtctg gtgaagacgg tggaaggcaa caagctgacg gtcgatttcg
     6001 acaaagcggg cgagaagaag gtgatcgatc agttcgtgac gagagggtga tgacaatcac
     6061 ttgttctttt tacctccccc aacgtgggga ggtcggcgag cgaagcgaga cgggtggggg
     6121 gatggtgcgg gtttcgacaa tccccccacc cgcccggctt cgcgctgcgc gctacgccgt
     6181 ggcgacctcc ccacaagggg gaggtgaaag atgatcaccc ctcccctctg gaaagcgtcc
     6241 gttacgctga cgaaagcgga agcgcatgat gttgccgcgg cgttcgagct gacgggcccg
     6301 caagctgtgc tgatcgctga agagccgttc accgatgagg ccgaggtgga agcgctgtac
     6361 ggcgtcatgc cggacggcga tctgctgtcg aggctggcgg ggcacgaggt acgcgtcatc
     6421 atcttgcccg acgccgactg gatcaagctt tcgcaggaag ggctaccgcc ggtgcgcgcg
     6481 gggcgattct tcgtctatgg cgcgcacgac gcgggcaagg ttccgcccgg cgtcattccg
     6541 atgcgcatcg aggcggggat ggccttcggc accggccatc acgaaaccac agcgctgtgc
     6601 cttgcggtgt tgagcgatct ggcgaagcgg cggcggttcg cgaaggtcct ggatttgggg
     6661 tgcgggacgg ggctgcttgc catcggcgct gcgaagctgt ggcggcggcg cgtgctcgcc
     6721 tcggacatcg atccggcggc ggtggaaatc acccgcgaga atgcggcggc caacggcgaa
     6781 gcgcctttcg tccgcgcggt ggcggccgac gggctgaccc atccggcgat cgccgaatcc
     6841 gcgccgttcg atctgatcgt ggccaacatc ctggccgggc cgctgacgct gctggccccc
     6901 gccatcgtcc gcgcgctcgc cccaggggga atgctggtcc tgtcgggcct gctgcaatgg
     6961 caggagaatc ttgttgccgg ctactaccgc ccgctcggcc tgattttgcg ccggaagcgg
     7021 cgggacggcg cttggtcggc gctggtattg gaacgcccaa atgcaccctt cgacaagctc
     7081 agggtgaagg attaatccaa accttcatgc tgagcctgtc gaagcatgag cctgtcgaag
     7141 catgagcttg tcgaagggtg agcgtaggcg ctaacctcgc gcgcatggac atatccttcc
     7201 aaaactatga cgaccagagc gacgcggcgg cttgcgcccc tcgtcttgcg gctctcagga
     7261 gcgaactcaa aaagcgcgga ctcgacggct tcctggtgcc g
//
LOCUS       DASWPU010000258         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_39206,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000258 DASWPU010000000
VERSION     DASWPU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39206"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VGC27_05570"
     CDS             <1..581
                     /locus_tag="VGC27_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0282073.1"
                     /translation="VYKPAHQERRPMDIRNIAIIAHVDHGKTTLVDQLLKQSGSVREN
                     QQMQERALDSNDLERERGITILAKCTSVEWGKTRINIVDTPGHADFGGEVERILSMVD
                     GVVLLVDAAESVMPQTKFVLSKALKLGLKPIVVINKVDRSDAQPAETLESIFDLFLAL
                     EANDDQLDFHVLYASGRQGWAVADLNDERKDL"
     gene            585..>1536
                     /locus_tag="VGC27_05575"
     CDS             585..>1536
                     /locus_tag="VGC27_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEY0282074.1"
                     /translation="MFARIVSDVPKPKPLTAAGDAFPFSMLVTTLEADPYLGRVLTGR
                     IETGAITVNRQIKALNREGTTVERARATKLLAFRGLARVPVERAEAGDIVAIAGLQTA
                     TVADTLCDVQVETPIYATPIDPPTLAMTVSVNDSPLAGREGDKVQSRVIRERLLREAE
                     GNVAIHVRETGEGAYEVAGRGELQLGVLIETMRREGFEVSISRPRVLYRTDEAGQRLE
                     PIEEVTVDVDDPYTGVVIEKISNRKGELTDMRPTGAGKTRLTFWAPSRGLIGYHGEFL
                     TDTRGTGVINRMFHGYAPYKGPVAGRTNGVLISTGDGEAVP"
BASE COUNT          304 a          493 c          484 g          255 t
ORIGIN      
        1 gcgtctataa gcccgctcat caagagcgca ggcccatgga catccgcaat atcgccatta
       61 tcgcacatgt cgaccacggc aagacaaccc ttgtcgatca gttgcttaag caatccggtt
      121 cggtgcgcga aaaccagcag atgcaggagc gcgcgctcga ttccaacgat ctggagcgcg
      181 agcgcggcat caccatcctg gccaaatgca ccagcgtgga atggggaaaa acccgcataa
      241 atatagtgga tacgcccggc cacgccgatt tcggcgggga agtggaacgc atcctgtcga
      301 tggtcgatgg tgtcgtcctc ctggttgacg ctgcggaatc ggtcatgccg cagaccaaat
      361 tcgtgctttc caaggcgctc aagctcggct tgaagcccat cgtcgtcatc aacaaggtcg
      421 accgatccga cgcccagccg gcggagacgc tggaatcgat cttcgacctg ttcctggcgc
      481 tcgaagccaa cgacgaccag ctcgatttcc atgttctcta cgcctcgggc cggcaaggtt
      541 gggcggttgc cgatctcaac gatgaacgca aggatttgta ggcattgttt gccagaattg
      601 tatcggatgt tcccaagccg aagccgctga ccgcggcggg cgacgctttt cccttctcga
      661 tgctggtgac cacattggag gccgatcctt atctgggccg cgtgctgacg ggccgcatcg
      721 aaaccggcgc catcaccgtc aaccgccaga tcaaggcgct gaaccgcgaa ggcacgacgg
      781 tagagcgcgc ccgggcgacc aaattgctgg cgttccgcgg cttggcgcgc gtgccggtgg
      841 agcgggccga ggccggcgac atcgtcgcca tcgccggttt gcagacggcc accgtcgccg
      901 acacgctgtg cgacgtgcag gtcgaaaccc cgatctacgc cacgccgatc gatccgccga
      961 ccctggcgat gaccgtttcg gttaacgatt cgcctctggc gggccgcgaa ggcgacaagg
     1021 tgcaaagccg cgttatccgc gagcggctgc tgcgcgaggc ggaaggcaat gtcgccatcc
     1081 acgtgcgcga aaccggcgaa ggcgcctatg aagtcgccgg ccgcggcgaa ctgcaactcg
     1141 gcgtgctgat cgagaccatg cggcgcgagg gcttcgaagt ttccatcagc cggccgcgcg
     1201 tgctgtacag gaccgacgag gccggccagc ggctggagcc gatcgaagaa gtcaccgtcg
     1261 atgtcgacga cccctacacc ggcgtggtga tcgagaagat ttcgaaccgc aagggcgagc
     1321 tgaccgacat gcgcccgacc ggcgcgggca agacgcggct gaccttctgg gcgccgtcgc
     1381 gcgggctgat cggctatcac ggcgaattcc tcaccgatac gcgcggcacc ggcgtgatca
     1441 accgcatgtt ccacggctac gcgccctaca aaggcccggt cgccgggcga accaacggag
     1501 tgttgatctc caccggcgac ggcgaagcgg tgccct
//
LOCUS       DASWPU010000259         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_57120,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000259 DASWPU010000000
VERSION     DASWPU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57120"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /locus_tag="VGC27_05580"
     CDS             complement(<1..1739)
                     /locus_tag="VGC27_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEY0282075.1"
                     /translation="MAKLEDLQPNAAVRGILPDSLITVVSAQWFGSEACELTYKDPAG
                     RVANTLLYRHDEPRLDVVEHGRPWSFDGDGAKFRLVSEAHRIRLAHLFDPVLAVHTSL
                     VDPLPHQITAVYEAMLPRQPLRFLLADDPGAGKTIMAGLLIKELIVRGDLKRCLIVCP
                     GSLAEQWQDEMSQRFHLPFEILTNDKLESARTGNWFAENDLIIARLDKLSRDVDVQAK
                     LTAPDCHWDLVVFDEAHKLSATFFGGEVKYTKRYRLGQLLSGFTRHFLMMTATPHNGK
                     EEDFQLFLALLDGDRFEGRFRDGVHQVDVSGMMRRMVKENLLKFDGRPLFPERIAYTV
                     PYALSDAEAQLYREVTEYVREEFNRAEALQNDKRAGTVGFALTVLQRRLASSSEAIYQ
                     SLRRRRERLEKRQRELELMQRGTLASTFGKELDPDDLEDLDDAPESELEAAEEEVLDQ
                     ATAASTIAELKVELAKLTHLESLAADLRRGQDTKWRELAPLLGTLFARGGATGDAADG
                     KAPYGSGSIPPPTPSPRQKLVVFTEHRDTLFYLERNIATLLGRQHAVVMIHGAMGRED
                     RRKAQESFLHDPDV"
BASE COUNT          405 a          697 c          683 g          439 t
ORIGIN      
        1 acgtcaggat cgtggaggaa gctctcctgc gctttgcgcc gatcttcgcg ccccatcgca
       61 ccatgaatca tcacaaccgc gtgttggcga cctagcagcg tcgcgatgtt tcgctctaga
      121 taaaacagcg tgtcgcgatg ctcggtgaac accaccagtt tctgtcgcgg ggatggggtc
      181 ggcggcggga tcgatccgga gccatacggc gccttcccat ccgcagcgtc accggttgcc
      241 cctccgcgcg cgaagagcgt ccccagaagc ggcgccagct cgcgccactt cgtgtcctgc
      301 ccgcgccgaa gatcggccgc gagcgactcc aagtgagtga gcttcgcgag ttccactttg
      361 agttccgcga tcgtactggc ggcggttgcc tgatcgagga cttcctcctc ggctgcttcg
      421 agttcacttt ccggcgcatc gtccaaatct tccagatcgt cggggtcgag ttccttcccg
      481 aaagtcgaag ccagcgtacc ccgctgcatg agttccagct cgcgttgccg cttctccagg
      541 cgctctcggc gacgacgcaa cgattggtag atcgcctcgg aagacgaggc cagtcggcgc
      601 tggagcacgg tgagagcaaa cccgacggtt ccagcgcgct tgtcgttttg caacgcttcg
      661 gcgcgattga actcctcgcg cacgtactcc gtcacttccc tgtagagctg cgcctcggca
      721 tccgaaagcg cgtatggaac tgtgtaagcg atgcgctctg ggaagagagg cctgccgtcg
      781 aacttgagga ggttctcctt caccatccga cgcatcatgc cgctgacgtc aacctggtgg
      841 acgccgtcac ggaatcttcc ctcaaagcgg tcgccgtcga gcagggccag aaagagttgg
      901 aagtcctcct ccttcccgtt gtgcggcgtg gccgtcatca tgaggaaatg ccgcgtgaag
      961 cccgagagca gctggccgag gcggtaccgc ttcgtgtact tgacctcccc gccaaagaac
     1021 gtggctgaca gtttgtgcgc ctcatcgaac accacgagat cccagtgaca gtccggcgcg
     1081 gtgagtttcg cctgcacgtc cacatcccgc gacaacttgt ccaggcgggc gatgatgaga
     1141 tcgttttcag cgaaccaatt ccctgtccgt gctgactcca acttgtcgtt cgtcagtatt
     1201 tcaaatggca ggtgaaagcg ttgactcatt tcgtcctgcc actgctcggc gagactgccc
     1261 gggcagacga tgaggcatcg tttcagatcg ccgcgaacga tcagctcctt gatgaggagg
     1321 ccggccatga tggtcttgcc tgccccaggg tcgtccgcga gtaggaagcg gaggggctgg
     1381 cgcgggagca tggcctcgta aaccgcggtg atctggtgcg gaagcgggtc gacgagcgac
     1441 gtgtgcacgg cgagcacagg gtcgaacaag tgtgcgagac ggatgcggtg agcctccgac
     1501 acgagacgga atttcgcgcc gtcaccgtcg aagctccacg ggcgaccatg ttcgacgacg
     1561 tcaaggcgag gctcgtcgtg ccggtaaaga agcgtgttcg cgacgcgccc tgcggggtcc
     1621 ttgtaggtca gttcgcacgc ctcggaccca aaccactgcg cgctcactac ggtgatgagg
     1681 ctgtcgggca gtattccgcg aacagcagcg ttgggctgga gatcctcgag tttggccatt
     1741 gggggattgt ggtggagcct ctgggtgacc gctccacaac cggcctccgg acggggcggc
     1801 acctaggcgg tgtggggcgg actgtggatt ctatcgcggc cgctagtccg cgtccagaac
     1861 ccccatttgg tcgatggaag gcaagaacga ggtccccagg tgctccgcgg gaagggatgc
     1921 cgtccagcgg ctcctcgccg ggaacgatca gagtttcgca cggtttcttt gcagagaacg
     1981 caaacaggcg gggtttcgca ctccagacaa ctatctaatt tcagaatagc gacttaatgg
     2041 tctttcccgc gttacgcttc ggcgcgtccg acgtgcttca acgcgtgcgc cccatggata
     2101 cccatcatct tcggcacgcc ctcccctgac gcgttcgcgc cgctcgcaca cgctcctacc
     2161 ccgactcggt gtatttggga caggaccacc catgacaccg cgcaagccat cgaatccgac
     2221 ttca
//
LOCUS       DASWPU010000260         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_39252,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000260 DASWPU010000000
VERSION     DASWPU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39252"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VGC27_05585"
     CDS             <1..1214
                     /locus_tag="VGC27_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY0282076.1"
                     /translation="HFDLIVLGSLNEGSWPASATDDPWFSRPMRRTLGLEQPERAIGL
                     SAHDFATLAAGPRVVLTRARKAEGAPTVASRWIQRLVQLARGLKIEERLAPRTPYRQI
                     LARLSDPGKPNPIAPPAPKPPVSARPRKLSFTEIETWVRDPYAIYAKRILRLRPLDPL
                     EAEIGPLERGSALHRALELFVRRFPHDLPPDAAMHLVAIAEEVFDKLGTPKATLAIWR
                     PRFLRAAAWFVEQERKRRPVIAESFVEINGERAFKGPAGEFILYGRADRIDRLRAGGG
                     AILDYKTGVPPTDPQVLKHLAPQLPLEGAVLMAGGFKEIGPLDPAELVYVRITGGAEA
                     GVFKPVKTDAKVIANQAAERLARRIAEFDGEGKAYESRVGPLFAKYQSDYDHLARVRE
                     WSASGWRGESE"
     gene            1211..>1680
                     /locus_tag="VGC27_05590"
     CDS             1211..>1680
                     /locus_tag="VGC27_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0282077.1"
                     /translation="MSKDSLAASDPRRSAWVAANAGAGKTHTLANRVTRLLLAGARPE
                     RILCLTYTKAAAAEMAGRLFDQLGRWSMLPDDELAKNIAEVGAEIGGKEELKKARRLF
                     ADALETPGGLKIQTIHAFCQYVLMRFPLEAGVPASFRVLDERTARDLMAEARARV"
BASE COUNT          296 a          553 c          565 g          266 t
ORIGIN      
        1 aacatttcga tctgatcgtt ctgggcagct tgaacgaagg ttcatggccc gcgtcggcca
       61 cggacgatcc gtggttttcg cgcccgatgc gccgcacgct gggcctggaa cagcccgaac
      121 gcgccatcgg cctttcggcg cacgatttcg ccacgctggc ggccggtccc cgcgtcgtgc
      181 tcacccgtgc gcgcaaggcg gaaggcgcgc cgaccgtcgc ttcgcgctgg attcaacgcc
      241 tggtgcaact cgccagaggc ttgaagatcg aagagagact ggcgccgcgg acgccttatc
      301 ggcaaatctt ggcgcggctg tccgatcccg gtaaacccaa tccgatagcg ccgcccgcgc
      361 cgaagcctcc ggtttcggca aggccgcgca agctctcctt caccgaaatc gaaacctggg
      421 tgcgcgatcc ttatgcgatc tacgccaagc gcattctgag gctgaggccg ctcgacccgc
      481 tggaggcgga gataggcccg ctggaacggg gtagcgccct gcatcgcgcg cttgaactct
      541 tcgtccgccg cttcccccac gacctgccgc cggacgccgc catgcacctc gtcgccatcg
      601 ccgaggaagt attcgacaag ctgggcacgc cgaaagctac gctggcgatc tggcggccgc
      661 gtttcctgcg agcggcagcc tggtttgtgg aacaggaacg caagcgccgc cccgtcatcg
      721 ccgagagctt tgttgagata aacggtgagc gtgccttcaa gggaccggcg ggagagttca
      781 tcctctacgg ccgcgccgac cgcatcgacc ggctgcgcgc aggcggcggc gcgatcctcg
      841 attacaagac gggcgtaccg cctaccgatc cgcaggtgct gaaacatctg gcgccgcaac
      901 tgccgctcga aggcgcggtg ctgatggcgg gcggcttcaa ggagatcgga ccgctcgatc
      961 cggcggaact ggtttatgtc cgcatcaccg gcggcgcgga ggcgggcgtg ttcaaacccg
     1021 tgaagaccga cgccaaggtg attgcaaacc aagcggcgga gcggctggcg cggcgcatcg
     1081 cggagttcga cggcgaaggc aaggcgtatg aatcgcgcgt cgggccgctc ttcgccaaat
     1141 accaaagcga ctacgatcat ctggcgcggg tgcgcgaatg gtcggcgagc ggctggcggg
     1201 gagagagcga atgagcaagg actcgctcgc cgcttccgat ccgaggcgct cggcctgggt
     1261 ggccgccaat gccggcgcgg gaaagacgca tacgctcgcc aatcgcgtga cgcggctgct
     1321 gctggccggc gcgcgccctg aacgcatcct gtgcctcacc tataccaagg ccgcagcggc
     1381 ggagatggcc ggacgcctgt tcgatcagct cggtcgctgg tcgatgctgc ccgacgacga
     1441 gctggcaaag aacatcgccg aggtgggcgc ggaaatcggc ggcaaggaag aactgaagaa
     1501 agcgcgccgt ctgttcgccg acgccctgga aacgcccggc gggctgaaga tccagaccat
     1561 ccatgccttc tgccagtatg tgctgatgcg ctttccgctg gaagcaggcg tgccggcctc
     1621 gttccgtgtg cttgacgaac gcaccgcgcg cgacctgatg gccgaggcgc gcgcccgcgt
//
LOCUS       DASWPU010000261         4318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_45315,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000261 DASWPU010000000
VERSION     DASWPU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45315"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..2064
                     /locus_tag="VGC27_05595"
     CDS             307..2064
                     /locus_tag="VGC27_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0282078.1"
                     /translation="MYVLGVNAFHGDSSAALIRHGRLLAAAEEERFRRVKHWAGFPSH
                     AIAYCLREAGIRLGDVDHIALNQDNRANLARKLAYLLRRRPASALVLRRLQNRSRRQG
                     VPELLAGRFPGEPFRGKFHNVEHHVCHLYSAYYVSPFPEAAIASVDGFGDFASTAWGA
                     GRGSRVVIDGRVYFPHSLGVFYQALTQYLGFPYYGDEFKVMGLAPYGKPATLDAMRRI
                     VNLKPGGRFELDLSYFRHHAEYVPYQWEGGAPEIGDLFSPALEDLLGPRRRAGEPIEE
                     RHRDIARSVQAMYEEAFFNLLVPLQQRTGLTNLTLAGGCAANSVANGKVRRMTPFRRV
                     YVQSAAGDAGGAIGAAYAVWDKLSGGRCFIVDHAFWGPQFAQSDIASLIDARRAEIES
                     AGCAVEEIADEEGLCRRTAKQVAEGKVVGWFQGRMEWGPRALGNRSIVCDPRRADMKA
                     ILNAKIKRRESFRPFSPSVLSGAVGEWFEEDDAVPFMMQVFQVREDKRAQIPSVTHVD
                     GSGRLQTVYKHTNPRFYRLIEAFRDISGVPMVLNTSFNENEPVVCEPKEALDCFLRTK
                     MDVLVLGNAMIARREGATA"
     gene            complement(2061..3194)
                     /locus_tag="VGC27_05600"
     CDS             complement(2061..3194)
                     /locus_tag="VGC27_05600"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0282079.1"
                     /translation="MAAPIPFIDLQAQRRRLGEPLNKAIAAAVEGGAWIMGPQVATFE
                     AQLAEFAGVKHAVACANGTDALLIVLMAWDIGPGDAVFVPTFTFAATGEVVALAGAAP
                     VFVDVLEDTYNMDPASLEAAIALVKREGKLKPRAVIPVDLFGQPADYRALEPIARREG
                     LKMLCDTAQAFGARYHGKVTGSIGDAAATSFFPAKPLGCYGDGGASFTNDDGLAEILK
                     SIHNHGAGTDRYENVRIGMNSRLDTIQAAILIEKLKIFPEEIDLREKVAQRYNAALGK
                     SNRIRVPVVIEGAQSTWAQYTIQVPDRPKLQADLKDKGIPTAVYYPIPMSAQKGYAKY
                     PSAPVPVAEKIGKTVVSLPMHPYLDEATQDRIVEAVLESVGGG"
     gene            complement(3377..>4318)
                     /locus_tag="VGC27_05605"
     CDS             complement(3377..>4318)
                     /locus_tag="VGC27_05605"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0282080.1"
                     /translation="FIGYHVAEALLARGREVAGVDNLNDYYEVSLKQARLSRLEARKG
                     FTFRKLDFADRDGMAALGAAFPGITQIVHLGAQPGVRYSLINPYAYIASNVMGQVTVL
                     ELARTLKNLRNVVYASSSSVYGGNEKTPFAVGDAVETPQSLYAATKRADELISHTYAH
                     LYGIKLAGLRFFTVYGPWGRPDMAPMIFARAILAGEPIRVFNNGEMWRDFTYIDDIVA
                     GVLAALDAPPAGKPPHKLYNLGNNRCEKLTDFIAALETALGKKAEMRFEPMQPGDVEK
                     TYADIEATRRELGFSPTTPISAGVPKFVEWYREFYRV"
BASE COUNT          735 a         1386 c         1389 g          808 t
ORIGIN      
        1 gggacggaga agcgcctgaa tccgttgaaa aaactgcacg gtcccgctaa gccgcgagaa
       61 gccgccggct cttttctccg gatagaagac tgcctcaaga tcggacaaac ccattcaccc
      121 cctgatggac gggcgggaat atagcaacgc cgtcgagcgg acgccaatcc gggagccggc
      181 tttgatacgg accttgcggc cggagaggcg ggcagaacgg ccgcgagtct ccccgcgacg
      241 accttgccgc tctgttcttg acgataagtg cgagtatgct ggcgcgcttt tcgatgcggg
      301 cgcgaattgt acgttctcgg cgttaatgcg tttcacggcg actcttcggc ggcgctgata
      361 cgtcacggcc ggctcctggc cgccgcggaa gaagagcgct tccgccgcgt caagcactgg
      421 gcgggctttc cgtcgcacgc gatcgcttat tgccttcgcg aagccgggat acgcctcggc
      481 gacgtggacc atatcgcgct caaccaggac aatcgcgcaa atctggctcg caagctcgcc
      541 tatctgttgc gccggcggcc ggcctccgcg ctggttctcc gtcgtttgca aaaccggagc
      601 cgacggcagg gcgtgccgga actgctggcc ggccggtttc caggcgagcc tttccgcggc
      661 aagttccaca atgtcgaaca ccatgtctgc catctctact cggcctatta cgtgtcgccg
      721 tttcccgaag ccgcgatcgc gtcggtggac ggcttcgggg atttcgccag cacggcttgg
      781 ggcgcaggcc gaggcagcag ggtcgtcatc gacggccggg tctattttcc ccattcgctc
      841 ggcgtcttct atcaggcgct gacccaatat ctcggatttc cctattacgg cgacgaattc
      901 aaggtgatgg gcttggcgcc ctacggcaaa ccggccaccc tcgacgccat gcggcggatc
      961 gtgaatctga aacccggcgg ccgcttcgag ctcgatcttt cctatttccg gcatcatgcg
     1021 gaatacgttc cctatcaatg ggagggcggc gcgcccgaga tcggcgatct cttttcgccg
     1081 gcgctggaag acctcttggg accgcggcgc cgcgccggcg aaccgatcga agagcggcat
     1141 cgcgatatcg cccgctcggt gcaggcgatg tacgaggaag ccttcttcaa tctgctggtc
     1201 cccttacagc agcgcaccgg gctgacgaat ctcaccctgg cgggcggctg cgccgcgaat
     1261 tcggtcgcca acggcaaggt gcggcggatg acgccgtttc gccgcgtcta tgtgcaatcg
     1321 gccgcgggcg acgcgggcgg cgcaatcggc gccgcgtatg ccgtgtggga caagctgagc
     1381 ggcggccgct gcttcatcgt cgatcatgcg ttctggggtc cgcaattcgc ccagtccgac
     1441 atcgcctcgc tgatcgacgc ccgcagggcg gagatcgagt cggccggctg cgcggtcgag
     1501 gagatcgcgg acgaagaggg gttgtgccgg cggaccgcga aacaggtggc cgagggaaag
     1561 gtcgtcggct ggttccaggg ccgcatggaa tgggggcccc gcgcgctcgg caaccgctcc
     1621 atcgtctgcg acccgcgccg cgcggacatg aaggcgatcc tcaatgccaa gatcaagcgc
     1681 cgcgagtctt tccgtccctt ctcgccctcg gtgctgtcgg gcgcggtcgg cgaatggttc
     1741 gaagaggacg acgccgtgcc gttcatgatg caggtgttcc aggtccgcga agacaaacgc
     1801 gcgcaaattc cctcggtcac ccatgtcgac ggttcggggc ggctgcaaac cgtttacaag
     1861 cacaccaatc cgcggtttta ccgcctgatc gaggccttcc gcgatatctc cggcgtaccg
     1921 atggtgctca acacctcgtt caacgagaac gagccggtgg tctgcgaacc gaaggaggcc
     1981 ttggactgct tcctgcggac gaaaatggac gttctggtgc tcggcaacgc catgatcgcg
     2041 cggcgcgaag gggcgacggc ctagccgccg cccacgcttt cgagcacggc ctcgacgatc
     2101 cggtcctgcg tcgcctcgtc cagataagga tgcatcggca ggctgacgac cgtcttgccg
     2161 atcttctcgg ccacgggcac gggcgcgctc ggatatttcg cgtaaccctt ctgcgccgac
     2221 atcgggatcg gataatagac cgccgtggga attcccttgt ccttcagatc ggcttgcagc
     2281 ttggggcggt cgggcacctg gatcgtgtat tgcgcccagg tcgattgcgc gccttcgatc
     2341 acgaccggca cgcggatgcg gttggatttg cccaatgctg cgttgtaacg ctgcgccacc
     2401 ttctcgcgca ggtctatctc ttccgggaat atcttcagct tctcgatcag gatcgcggcc
     2461 tggatcgtat cgaggcgcga attcatcccg atgcggacat tctcgtagcg gtccgtgccc
     2521 gcgccgtgat tgtggatgga tttgagaatt tcggccagcc cgtcgtcgtt ggtgaagctg
     2581 gcgccgccgt cgccgtagca gcccagcggc ttggccggga agaaactggt ggccgccgcg
     2641 tcgccgatgc tgccggtgac ctttccgtga tagcgcgcgc cgaaggcctg cgcggtgtcg
     2701 cacagcatct tcaaaccttc gcgccttgcg atgggttcca gggcgcgata gtcggccggc
     2761 tggccgaaca gatcgacggg gatgacggcc cgcggcttca gcttgccttc gcgcttgacc
     2821 aaggcgatgg cggcttcgag gctggccgga tccatattgt aggtatcttc gagcacgtcc
     2881 acgaacaccg gcgccgcgcc ggccagcgcc acgacttctc cggtggcggc gaaggtgaag
     2941 gtgggcacga acaccgcgtc gcccggcccg atgtcccacg ccatcaagac gatcaacagc
     3001 gcatccgtgc cgttggcgca ggcgacggcg tgcttgacgc ccgcgaactc ggcgagttgc
     3061 gcctcgaagg tggcgacttg cggccccatg atccaggcgc cgccctcgac cgccgccgca
     3121 atggccttgt tcaagggctc gccaagcctg cggcgctggg cctgaagatc gatgaaagga
     3181 atcggcgcgg ccatgggacc tccggtgcgg actttgccgg ccgttctaga acccgttgcc
     3241 gccgcagacg atgaaagaaa tgttgcagat tgcgtcaggc attacctttt taaccactgt
     3301 cgcgctcagc caacgcccgt ttgcgggatg gtgctgcacg cccagataga acgggcgccg
     3361 tctccgccgc ccgcgtctag acacggtaaa actcccgata ccattccacg aatttcggca
     3421 cgcccgcgct gatcggcgtg gtcggcgaaa agccgagttc gcggcgcgtg gcctcgatgt
     3481 cggcataggt cttctccacg tctccgggct gcatcggttc gaagcgcatc tcggccttct
     3541 tgcccagtgc ggtttcgagc gcggcgatga agtcggtgag cttctcgcat ctgttgttgc
     3601 cgagattata gagcttgtgc ggcggcttgc ccgcaggcgg ggcatccagc gcggccagaa
     3661 cccccgccac gatatcgtcg atataggtga aatcgcgcca catctcgccg ttgttgaaga
     3721 cgcggatcgg ctcacccgcc aaaatagccc gcgcgaagat catcggcgcc atgtcggggc
     3781 ggccccaagg tccgtagacg gtgaagaagc gcagccccgc cagcttgatg ccgtagagat
     3841 gggcgtaggt gtgcgagatc agttcgtccg cgcgcttggt ggcggcataa agcgattggg
     3901 gcgtctccac cgcgtccccc accgcgaagg gcgtcttctc gttgccgcca tagaccgagg
     3961 acgaactcgc ataaaccacg ttgcgcaggt tcttgagcgt acgggccagc tccagcaccg
     4021 tcacctgccc catcacgttc gaagcgatat aggcgtaagg attgatcagg gaatagcgca
     4081 cgcccggctg ggcgccgaga tgcacgatct gcgtgatgcc gggaaaggcg gcgcccagcg
     4141 ccgccatgcc gtcgcggtcg gcgaaatcga gcttgcggaa ggtgaagcct ttcctggctt
     4201 cgagccggct cagccgcgcc tgtttcaggc ttacctcgta atagtcgttg agattgtcca
     4261 ccccggccac ttcgcgcccg cgcgcgagca gcgcctcggc gacatggtac ccaatgaa
//
LOCUS       DASWPU010000262         5529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_81861,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000262 DASWPU010000000
VERSION     DASWPU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81861"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..676)
                     /locus_tag="VGC27_05610"
     CDS             complement(125..676)
                     /locus_tag="VGC27_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282081.1"
                     /translation="MSLSSKEAAETLSDVERASRRSAQLYGYSRSAPHLIMWGSIWVV
                     GYTQTDLFPRYANWGWIALILAGVIGGAIIGQRNAALKHGPLAWRMFAVSAIAIFFVF
                     ATYTIMWPVRGEAIAAYPALITGTLYAGIGLWAGLRYVITGIVVVAATLGGYFFLHEH
                     LLLWMAFVGGGGMILAGIWFRKV"
     gene            complement(789..1490)
                     /locus_tag="VGC27_05615"
     CDS             complement(789..1490)
                     /locus_tag="VGC27_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282082.1"
                     /translation="MKSVLLAAATAGALLGGGALAMRIADAPQADGAVLHPQRIVIGL
                     DLSRSNPLIANPVFASKVSARIAGIVHDLGFASEVHVRTFGNYDASSNNWSYDVVLSV
                     RNRPENVAAEVQRLIAGTPLLVRTGKWRAQDNTNILAFLDNVSHSIGCAGMPTTIILA
                     SDGLEDSDYARLRQADGHLPAPDGRPFARCARLEILGLGQGAGSPRLTARLRGEWSGW
                     ARAAGFAQFVGLNDW"
     gene            complement(1506..2603)
                     /locus_tag="VGC27_05620"
     CDS             complement(1506..2603)
                     /locus_tag="VGC27_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282083.1"
                     /translation="MTAYNKPRDDKKPRTRQALSHDLLHGLTPEEFDNEAKALAAAEA
                     MTPAVRARLKTLLIERLKTPVDQPSWGPDWVRHGERVAARNRRGKGVVGKLLTFGSKK
                     KTEDEAPPGPLSEEETDAAAVIARNMEKSRQVSHYKPLYAIAAGLALAIMLVALFVDY
                     HIIREVWTRALANEFMVVPPALQSSVVFKSLQVIFAVLIVHFMLKITGAYGRNTMIAA
                     AFVMAVVMIGGLGYLVAYNNMAGATSATMERQRTQPNNSIDALFASTGEVQSAALTRL
                     APQSAVANPTEGVSLGLPKLSQASLADADSWFWLAFASVIFFIVTIVAALYMQIIENN
                     ARNFHIAHDYKHRQKQFTQLHLLELADRPRA"
     gene            3115..3438
                     /locus_tag="VGC27_05625"
     CDS             3115..3438
                     /locus_tag="VGC27_05625"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY0282084.1"
                     /translation="MPNDHAIDRLFATIAARKTADAGTSYTAKLLKDGVERCAKKFGE
                     EAVEAAIAAVSGKKDATVAESADVLYHLLVLWAACGIEPEEVYAALAAREGRSGLEEK
                     ASRPS"
     gene            3446..4147
                     /locus_tag="VGC27_05630"
     CDS             3446..4147
                     /locus_tag="VGC27_05630"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY0282085.1"
                     /translation="MRVLLTRPKQDAGVFTGRLRQLGHEALSAPLLHVRFHAGEPLAL
                     DGVQAILVTSANGVRALARRTKRRDLPVFAVGPQTAQEALSAGFARVESAEGDAVALA
                     EALPRWARADAGSLLHATGAQGAGRLASLLAAKGYQVITEALYDVVASAALPQDVANA
                     LEGGSLDAALFFSPRSAGVFRDCVLKAALAGACAGLIAVCISRTAAEALAPLSFREIR
                     IAARPNQDAVLACLG"
     gene            4192..5145
                     /locus_tag="VGC27_05635"
     CDS             4192..5145
                     /locus_tag="VGC27_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0282086.1"
                     /translation="MLRNFFLGIAAVVVAALCGAYFVFSQPDIPRRLLEAKYAGAPSQ
                     FVLLKDGARVHYRDRGPADAPVLVLLHGANASLFVWEPWSKILSDRFRVISVDLPGHG
                     LTGAVPNGDYSEAGMAEFVRAFADKLGLKRFALAGNSMGGGIAARFAELYPDRVTHLI
                     LVNAVGMQSQFGGRIPWMFRLARVPVVNRLLLHITPRSLVVEALNDAVARKAVLTDQM
                     IDRYWDFARMTGTRQAMFTRFRPPPDDFVRGHVGAIKAPTLILWGAQDRLIPVAAAHA
                     WAKAIPGSKLAIYPDTGHLPMEEVPRESAGDVRAFLDTQAR"
     gene            5245..>5529
                     /locus_tag="VGC27_05640"
     CDS             5245..>5529
                     /locus_tag="VGC27_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282087.1"
                     /translation="MDRLSAVIRPGRDAPYRNYVLGAAYDEMFAADGSVRSHYAALDS
                     RLTTLAPEELYRRQQACELSFLHQGITFTVYSDKQATERIIPTDLLPRIVT"
BASE COUNT         1031 a         1724 c         1735 g         1039 t
ORIGIN      
        1 gtcgccgcca cgatggcctt caagcggacg aattcgatcg cttcgccggc gggcagcacc
       61 ttgagcgcgg acataatctt gaggcgcacg ggctgatgga tgatgggatt cggctgttcc
      121 atggtcatac cttacggaac cagatgccgg ccaggatcat cccgccgccg ccgacgaagg
      181 ccatccacag cagaaggtgt tcgtggagga agaagtagcc gcccagcgtg gcggcgacca
      241 ccacgatccc cgtgatcaca taccgaagtc cggcccacaa accgattccc gcgtagagcg
      301 tccctgtgat cagcgccggg taagccgcta tggcttcgcc gcgaacgggc cacataatcg
      361 tgtaggtcgc aaacacaaaa aagatggcaa tcgctgacac cgcgaacatg cgccaggcaa
      421 gagggccgtg ttttagggcg gcgttgcgtt gcccgatgat cgcgccgccg atcacgcccg
      481 ccagaatcaa cgcgatccag ccccagttcg cgtaccgggg aaaaagatcg gtttgagtgt
      541 atccaacgac ccagatgctt ccccacatga tcaggtgcgg tgctgaccgg ctgtaaccgt
      601 aaagttgcgc gctgcgccga ctggcgcgtt cgacatccga tagggtttcg gcagcttctt
      661 tcgaggacaa agacatggtc agtgaaatcc caaatgaaaa cacagattac tctatactac
      721 agagttatct gagtcaacaa atgccgctat acatatctta tcgtttataa tcaatatgtt
      781 ggctgaagct accagtcgtt caagccgacg aactgagcaa acccggcggc gcgcgcccag
      841 cccgaccatt cgccgcgcag gcgggcggtc aagcgcgggc tgcccgcgcc ctggcccagg
      901 ccgagaatct caagtctggc gcaccttgcg aacggcctgc cgtcaggggc aggcagatgg
      961 ccatcagcct ggcgcaacct ggcataatcg gaatcctcaa gcccatcgct cgccaggatg
     1021 atcgtggttg gcatgcccgc gcagccgatg ctgtgggaaa cattgtcgag aaaggcgagg
     1081 atattggtgt tgtcctgggc gcgccatttg ccggtcctga ccaggagcgg cgtgccggcg
     1141 atgagccgct ggacttcggc ggcgacgttc tccgggcgat tgcgcaccga cagcacgaca
     1201 tcgtaggacc aattgttcga gctggcgtcg taattgccga aggtgcgcac atgcacttcg
     1261 gaggcaaagc ccagatcatg cacgatgccg gcgatgcgcg cgctcacctt ggaggcgaaa
     1321 acgggattgg cgatcaacgg attgctgcgc gacaggtcca gcccgatgac gatacgctgg
     1381 ggatgaagca cggcgccgtc cgcttgcggc gcgtccgcta tccgcatggc caaagcaccc
     1441 ccgccgagca gagcacccgc cgtcgccgcc gccagcaaga cgctcttcat ggtctcgccc
     1501 cctggttagg ccctcggccg gtcggccaac tcgagcaggt gaagctgggt gaattgtttc
     1561 tggcggtgtt tgtaatcgtg cgcgatgtga aagttgcgcg cattattctc gatgatctgc
     1621 atgtagagcg ccgccacaat tgtcacgatg aagaatatca cgctggcgaa ggcaagccag
     1681 aaccaggaat cggcgtccgc cagagaggcc tgggacagtt tgggaagccc gagcgaaacg
     1741 ccctcggtcg gatttgcgac ggcggactgg ggcgccaagc gggtcagggc agcgctctgg
     1801 acctcgccgg tggaggcgaa cagcgcgtcg atcgaattgt tcggctgggt ccgctggcgt
     1861 tccatcgtcg ccgaggtggc gcccgccata ttattgtagg cgacgagata gcccagcccg
     1921 ccgatcatca ccacggccat gacgaaagcg gcggcgatca tcgtattgcg gccgtaggcg
     1981 cccgtgatct tcaacatgaa atgcacgatc aggaccgcga agatcacttg caggctcttg
     2041 aacaccacgg aagattgtaa agccggcggc accaccatga attcgttggc cagcgcgcgc
     2101 gtccatactt cgcggatgat gtgataatcg acgaacaacg ccaccagcat gatcgccagc
     2161 gccaggccgg cggcgatggc ataaagcggt ttgtaatggg agacttggcg gcttttctcc
     2221 atgttgcgcg cgatgaccgc cgcagcgtcc gtttcctctt cgctgagcgg gccgggcggc
     2281 gcctcgtctt cggtcttctt cttgctgccg aaggtcagga gcttgccgac gacgcccttg
     2341 cccctgcggt tccgggcggc gacgcgctcg ccatgcctga cccagtccgg cccccagctc
     2401 ggctggtcga cgggtgtttt cagccgctcg atcagcaggg ttttcagccg ggcgcgtacc
     2461 gcgggcgtca tcgcttcggc cgccgccagc gccttggctt cgttgtcgaa ttcctccgga
     2521 gtcaaaccgt gcagaagatc gtgtgaaagt gcttggcgtg ttcttggttt tttatcgtcg
     2581 cgtggcttgt tgtaggcggt catgaccgtc ccccagtcgt ttcctctcgc gaaccctcgc
     2641 tttatagtaa acaactcgtt tcacgaccta tttgaggaag gattgaggca ggactgccac
     2701 aattaagaag aaacaaagat gcgcgcaggt cttggcgtgt gggcaattac cgtggcgatg
     2761 tcgctatttc ccttcgccgc gaccgcggac ccgaaggaga accttctagt gtcccgattc
     2821 cgaaattcgc accatctcta tatcggcctc gggcgaattt cggaatcgaa aggacactag
     2881 caacctattg aatcctagtg tcgttttggt ttcgaagttc gcttgcagcc cgatagtgcg
     2941 catgttgcga acttcgaaac cacgacacta gccgccggca taagcaccga agatccggcg
     3001 ccgagtgccg caccctgaaa gcatgcgccg cgcggcggac tcggttatgt tcggggccat
     3061 actgccccat tctgcgctcc gccgccggcg gcggctgaag gaacgaggaa caagatgccg
     3121 aacgatcacg caattgaccg gctcttcgcc accatcgccg cgcggaagac ggcggatgcc
     3181 ggcacttcct acaccgccaa gctgttgaaa gacggcgtcg aacgctgcgc caaaaaattc
     3241 ggcgaggaag cggtggaggc cgccatcgcc gccgtgtcgg gaaagaaaga cgcgaccgtc
     3301 gccgaatccg ccgacgtgct ctaccacctg ctggtgctgt gggcggcgtg cgggatcgaa
     3361 cccgaagaag tctatgccgc gctcgccgcg cgcgagggac gctccggtct ggaggaaaag
     3421 gcatcgcgcc catcctgagg aagccatgcg cgttcttctc acccggccca agcaagacgc
     3481 gggtgttttc accggacgct tgcgccaact cggccatgag gcgctgtcgg cgccgctgct
     3541 gcatgtgcgg ttccacgcag gcgaaccgct cgccttggac ggcgtgcaag ccatcctggt
     3601 caccagcgcc aacggcgtgc gcgccttggc gcggcggacc aagcggcgcg acctgccggt
     3661 tttcgcggtc gggccgcaga ccgcgcagga ggcactgagc gccggttttg cgcgcgtcga
     3721 aagcgcggag ggcgacgccg tggcgctggc cgaggcgttg ccgcgctggg cgcgtgccga
     3781 tgcgggcagc ttgctgcacg cgacgggcgc gcaaggcgcg ggccgtttgg cttcgctcct
     3841 cgccgccaag ggatatcaag tgattaccga agccctctac gatgtcgtgg cttccgccgc
     3901 tttgccgcag gacgttgcca acgcactcga aggcgggtcg ttggacgcgg cgctgttctt
     3961 ttcgccgcgc agcgcgggcg tattccggga ttgcgtcctc aaggcagccc ttgcgggtgc
     4021 ctgcgccggg ctcatcgcag tgtgcatcag caggaccgcg gcggaagcgc tggcgccgct
     4081 gtccttccgg gaaatccgca tcgccgcgcg gcccaatcag gacgccgtgc tggcatgtct
     4141 gggctgaccc atatcgtgta caatcgggct gggcaaaagt aggggcggca gatgctgaga
     4201 aacttttttc tcgggattgc ggcggtcgtc gtcgcggcgc tgtgcggtgc ctatttcgtt
     4261 ttcagccagc ccgacattcc gcgccgcctg ctggaagcca aatatgccgg cgcgccctcg
     4321 cagttcgttc tcttaaagga cggtgcgcgc gtccattacc gcgaccgcgg accggcggat
     4381 gcgccggtat tggtgctgtt gcacggagcg aatgcttcgc tgttcgtctg ggagccttgg
     4441 tcgaagattc tgtccgacag gtttcgcgtc atctccgtcg atttgcccgg ccatggcctg
     4501 accggcgccg tgccgaacgg cgattattcc gaagcgggca tggcggagtt cgtgagagcc
     4561 ttcgccgaca agctgggact gaaacggttc gcgctcgccg gcaattccat gggcgggggc
     4621 atcgccgcgc gctttgccga actctatccg gaccgtgtca cccatcttat tttggtgaac
     4681 gccgtcggca tgcagagcca gttcggcggt cgcattccat ggatgttccg tctggcgcgc
     4741 gtgcccgtcg tcaaccggct gctgctgcac atcaccccgc gttcgctggt ggtggaagca
     4801 ctgaacgacg ccgtcgcccg caaagcagtc ctcaccgatc agatgatcga ccggtattgg
     4861 gatttcgccc gcatgacggg gacgagacag gcaatgttca cccgcttccg cccgccgccc
     4921 gacgatttcg tgcgcggcca tgtgggagca atcaaggcgc cgaccctgat cctttggggc
     4981 gcgcaggatc gtctcattcc ggttgccgcc gcccatgcct gggccaaggc gattcccggt
     5041 tcgaagctgg cgatctaccc cgacaccggc cacctgccga tggaagaggt gccccgggaa
     5101 tcggccggcg atgtccgtgc ctttctggac acacaggctc gctagccctg cgttcgggca
     5161 gggagaagct taggaaaggc ttgacgaagg ggggcgccgc gcattcttat ttcgcaaatg
     5221 cagcatgaag cggcaataaa tacgatggat aggctttcgg ccgtgatacg gccgggccgc
     5281 gatgcacctt acaggaatta cgtcctaggg gccgcctatg acgagatgtt cgcggcggac
     5341 ggaagcgtcc gctcccatta cgctgcgctc gactccagac tgaccacgct tgcccccgaa
     5401 gaactctatc gccgccagca ggcctgcgaa ctttcctttc tgcaccaagg catcaccttc
     5461 accgtctaca gcgacaagca ggcgaccgag cgcatcatcc ccaccgatct gttgccgcgc
     5521 atcgtcacc
//
LOCUS       DASWPU010000263         4730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_2165, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000263 DASWPU010000000
VERSION     DASWPU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2165"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VGC27_05645"
     CDS             <1..621
                     /locus_tag="VGC27_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282088.1"
                     /translation="VREAVGPGFPIGAKLNSSDFQRGGFTSRECIELVRMLNDTSLDL
                     LELSGGSLEQPKVMGITLKEEGEDGPRQSTIAREAYFVNFAKDIRDVAKMPVMVVGGF
                     RSANAMIESLDKGELDVVGIARPMIAEPDGPRRLIAGEIERLPSFEKTFGIFHFMPWN
                     NMQLERMGDGLEPDLSLTGEAAATEFAQREGRNAAALLERRGRRAA"
     gene            666..1562
                     /locus_tag="VGC27_05650"
     CDS             666..1562
                     /locus_tag="VGC27_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0282089.1"
                     /translation="MKIFITGVSGYIGGSVAEKLVAAGHAVAGLVRSEEKVLSLKARG
                     IDTVIGTLDDADILAQAAEAADAVIHTANADHPGSVVTLVTALERSGKLLIHTTGSSI
                     VADHADGEYAAQAPLAEDDYFEPVPNRRSRVDMNRYVRQAAIEKGIRSVVICPTMIYG
                     RGRGLQPDSDQIPKLIALSRQLGAGVYFGKGLNRYSNVHIDDLADLYLLAMEKAPGGS
                     IFFAENGDNSFREIAGMMSRYLNFDDRTVSFPVENVIRQFGEAGRLGVASNSYVSAVN
                     ARRLGWSPKAPSLAEWFEGLQP"
     gene            1692..3527
                     /locus_tag="VGC27_05655"
     CDS             1692..3527
                     /locus_tag="VGC27_05655"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEY0282090.1"
                     /translation="MEQYSRRSMLKLGSTTVAAGVFAGRAVAAGAPQSAAPTLHLVRY
                     ADVDLLEGPLAEQFRSHHQTLLAMDEDALLKPFRKAAGLPAPGADLGGWYNASPHFNP
                     PADFHGFIPGHSFGQYLSGLARAYAITGDRATREKVRRLVAGFAPAVTPRFYDGYPLP
                     AYTFDKINIGLIDAHAFAADATALRVLNRATDAALPHLPTKALTRLEMEARPHSNPAF
                     TWDESYTLPENLYLAWRRGAGERYRTLARRFLLDRDYFDPLAAGENVLPGKHAYSHVN
                     AFSSAMQAYFVDGSAKHLAAAKNGFDFVLAQSFATGGWGPNEGFVVPGSGALGESLHM
                     THAGFETPCGSYGHFKAARYLMTTTGDNRYGDSMERVLYNAVLGALPMKPDGTAFYYA
                     DYNVAGAKVYHDYRCTCCSGTLGQIVADYGISAYFQSDRGVFVNLYVPSELRWKRGGR
                     TVTLRQTTQYPLAPEIVIAVRTTTPEDFSVFLRIPAWAGQGTAVAVNDRLWGAQAVPG
                     RFLEIHRTWRDDDRITLALDMPLRLEAVDDKHPNHLAVMQGPLALFAVGDRFLPFRRE
                     ELLTVRQVSPRAAEWRASTSDGVQVFKPYFAIGSETTRLYQIVPA"
     gene            complement(3594..>4730)
                     /locus_tag="VGC27_05660"
     CDS             complement(3594..>4730)
                     /locus_tag="VGC27_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282091.1"
                     /translation="VCILYMGLRLLGHGDLVAGWHATVASAGANIHLIRPLGDPDWSW
                     LAVLPGSAIIGFWYWCTDQYIVQRVLAGKNQKESRRGSILAAYLKLTPVFIFLVPGMV
                     AYALTKSDLGFATSGDAAYTSLVAQILPHGIRGIVACGMIVALMASLASKFNASATLF
                     TMDFFREWKPNASGRTEVVVGRIATTVIVVLGICWVLVIKSLHSNLYVYLQSVQGYLS
                     PAIAVLFVGGVFWKRATATAALWSFLVGVAGGFARLAADIYMRADEQFVKGLKDQLYL
                     HTITLDQYNAAIAPIRAKFGWIFEIWNIHWLYYCQILLVVTAVLMIAISYMTKAPDPK
                     TVKFTFYGASPEEKAATRKSWNALDVLLSAGVVGAIVCFYYYFW"
BASE COUNT          907 a         1452 c         1498 g          873 t
ORIGIN      
        1 gtccgcgaag cggtcggacc aggctttccg atcggcgcca agctcaactc ttccgatttt
       61 cagcgtggcg gtttcaccag cagggaatgc atcgaactcg tgagaatgct gaacgacacg
      121 agcctcgatc ttctcgaact ctccggcggc tcgctcgaac agccgaaggt gatgggcatt
      181 accttgaagg aagaaggtga ggatggtccc cgccagagca cgatcgcgcg tgaagcctat
      241 ttcgtgaact tcgccaagga tattcgcgac gtagccaaga tgccggtcat ggttgtcggc
      301 ggctttcgct cggcaaatgc catgatcgaa tcgctcgaca agggcgaact cgatgtcgtg
      361 ggcatcgccc gccccatgat cgcggagccc gacgggccga gacgtctgat cgccggcgag
      421 atcgagcgcc tgcccagctt cgagaagacg ttcggcatct tccacttcat gccgtggaac
      481 aacatgcagc tcgaacgaat gggcgacggt ctcgaaccgg atctgtcgct gaccggcgag
      541 gccgcggcaa cggagtttgc gcaacgcgaa ggcaggaacg cggcggcact gctcgagcgc
      601 cgcggccgcc gcgccgcctg aaccaccacg cgagataggt tcgacctgat gcagggagat
      661 gcagcatgaa aatattcatc acgggcgtat ccggctatat cggcggctcc gtcgccgaaa
      721 agctcgtggc tgcgggccac gccgtggcgg gtctggtccg atccgaagag aaagttcttt
      781 ctctgaaagc gcgcggcatc gatacggtga tcggtacgct ggacgacgcc gatattctgg
      841 cgcaagccgc cgaggcggcg gacgccgtta tccatacagc gaacgccgat catcccggtt
      901 cggtggtcac gctcgtcacc gctctcgaac gcagcggcaa gctgttgatc catacgaccg
      961 gctcgtccat cgtcgcagat catgccgatg gcgaatatgc agcccaggcg cccttggccg
     1021 aggacgacta tttcgagcct gttcccaatc gccggtcacg tgtcgacatg aaccgctatg
     1081 ttcgccaggc cgcgatcgaa aagggaatcc ggtcggtggt catctgtccg accatgatct
     1141 acggcagggg gcgcggcctg cagcccgaca gcgatcagat accgaaactg attgcgcttt
     1201 cccggcagct cggcgccggc gtctatttcg ggaagggcct taatcgttat tccaacgtcc
     1261 atatcgacga tctcgccgat ctctatctgc ttgcgatgga aaaggcgccc ggcgggtcca
     1321 tctttttcgc ggagaatgga gataattcct tccgcgaaat cgccggaatg atgagccgct
     1381 atctcaactt cgatgaccgg acggtcagct tccctgtgga gaatgtcatc cgccagttcg
     1441 gtgaagccgg ccgtctggga gtcgcatcca acagctatgt cagcgccgtc aacgcgcgac
     1501 ggctaggctg gtcgccgaaa gccccgtccc tggccgaatg gttcgagggc ctgcagccgt
     1561 gaaggaggtt caccaaggat tcggataaga gttcgagaac tgttggggcc caaatgtcag
     1621 aatgcgctga gaaggacaca tgatttgcgc catgttagaa tgaagcggga catgctctgt
     1681 ggggaccaca aatggaacaa tactctcgcc ggtccatgct gaaacttggg agcacgacag
     1741 ttgccgcagg tgttttcgcc ggccgggccg tggcggccgg cgcgccgcaa tccgccgcgc
     1801 cgacattgca tctggtccgt tacgcggacg tcgatctgct cgagggtccg ctggcggagc
     1861 aattccgctc tcatcaccag acgcttctgg caatggacga agacgccctg ctgaagcctt
     1921 tccgcaaagc cgccgggctg ccggccccgg gcgcggactt gggcgggtgg tacaacgcat
     1981 cgccgcattt caatccgccg gcggattttc acggcttcat tcccggccac agcttcggcc
     2041 agtacctgtc cggattggcc cgcgcctatg cgataacggg agatcgggcg acgcgggaaa
     2101 aagtgcggcg gctggttgcg gggtttgctc ctgcggtgac gccccgtttc tatgacggtt
     2161 atccgttgcc cgcctacacg ttcgacaaga tcaacatcgg gctgatcgat gcgcatgctt
     2221 tcgccgcaga cgcgacggcg ctcagggtcc tgaaccgggc gacggatgcg gcgctgccgc
     2281 acttgccgac aaaggccctg acgcgcctgg aaatggaggc gcggccgcat tcgaatcctg
     2341 cgttcacttg ggacgagtcc tatacgctgc cggaaaatct ctatctggcg tggcggcgcg
     2401 gcgcgggaga gcgctaccgc acactcgccc gccgctttct gctcgaccgc gactatttcg
     2461 atccgcttgc cgcaggcgag aacgtcctgc ccggcaaaca cgcctatagc cacgtcaatg
     2521 cgttcagctc ggccatgcag gcctattttg tggacggcag cgccaagcat ttggcggccg
     2581 cgaagaacgg cttcgatttt gttctggcgc agagctttgc caccggcggc tggggtccga
     2641 acgagggctt cgtcgttccc ggttcggggg cgctgggcga aagcctgcat atgacgcatg
     2701 ccggcttcga aacgccgtgc gggtcttacg gacatttcaa ggcggcgcgc tatctgatga
     2761 ccaccacggg cgacaaccgc tacggcgaca gcatggagcg cgtcttgtat aacgccgtgc
     2821 ttggcgctct tcccatgaag ccggacggga ctgctttcta ttacgccgac tacaatgttg
     2881 ccggcgccaa ggtctatcac gattacaggt gcacctgctg ctccggtacg ctgggtcaga
     2941 tcgtggcgga ttacggcatc agcgcctatt tccaaagcga ccggggcgtg ttcgtcaatc
     3001 tgtatgtgcc ttcggaacta cgctggaagc gaggcggccg cacggtcacg ctgcggcaaa
     3061 cgacgcaata cccgctggcg ccggaaatcg tcatcgccgt tcgcaccaca acgccggagg
     3121 atttctccgt tttcctgcgc atcccggctt gggcgggaca aggcacggcg gtggcggtga
     3181 acgaccggct atggggcgcg caggccgtgc ccggccgctt ccttgaaatc caccggactt
     3241 ggcgcgacga cgaccgcatc acgctcgcgc tcgacatgcc gctgcgcctg gaggcggtgg
     3301 acgacaagca tccgaaccat ctcgccgtca tgcagggacc gctggcgttg tttgcggtcg
     3361 gcgaccgttt cctgccgttc cggcgcgagg aattgctgac ggtccgtcag gtctcgccgc
     3421 gggcggcaga atggcgggcc tccacctccg acggcgtgca ggttttcaaa ccctatttcg
     3481 ccatcggcag cgagacgacg cggctctacc agatcgttcc tgcttaaaaa tcggcctgcc
     3541 ggatgggtct tccggcaggc cgatctgcag tgccgttcgc gttcttctgg ggcttaccag
     3601 aagtaatagt agaagcagac gatggcgccg acgacgcccg cgctcaacaa cacgtccaga
     3661 gcattccagc tcttgcgcgt ggcggccttt tcttccggcg aagcgccgta gaaggtgaac
     3721 ttgaccgtct tgggatcggg cgccttggtc atgtagctga tcgcgatcat caagaccgcc
     3781 gtgaccacca gcaggatctg gcagtaatag agccagtgga tgttccagat ctcgaagatc
     3841 cagccgaact tggcccggat cggcgcgatc gcggcgttgt actggtcgag cgtgatggtg
     3901 tgcagataga gctggtcttt gaggcccttc acaaactgct cgtcggcgcg catgtagatg
     3961 tcggcggcga ggcgggcgaa gccgccggcg acgcccacca ggaacgacca cagcgccgcc
     4021 gtcgcggtgg cgcgtttcca gaatacaccg ccgacgaaca gcacggcgat ggccggcgat
     4081 aaataaccct gtacgctctg cagatagacg tagaggttgg agtgcaggct cttgatcacc
     4141 agcacccagc agatacccaa gaccacgatg accgtcgtgg cgatgcgccc gacgaccact
     4201 tcggtcctgc cggaagcgtt cggcttccat tcgcggaaga agtccatcgt gaacagcgtc
     4261 gccgaggcgt tgaacttcga cgccagcgag gccatcaggg ccacgatcat gccgcaagcc
     4321 acgatgcctc tgatgccgtg cggcaggatc tgcgcgacca gcgaagtgta ggcggcgtcg
     4381 ccgctggtgg cgaacccgag gtccgatttg gtcagggcat aagcgaccat gccgggcact
     4441 aggaagatga agaccggcgt cagcttcaga taggcggcca ggatggagcc gcgccgcgat
     4501 tccttttgat ttttgccggc caggacgcgc tggacgatgt actggtcggt gcaccagtac
     4561 cagaagccga taatggccga gccgggcagc accgccagcc aggaccaatc gggatcgccg
     4621 agcgggcgga tgagatggat attggcgccg gccgacgcga ccgtggcgtg ccagcctgca
     4681 accaggtcgc cgtgcccaag caggcgcagc cccatgtaaa gaatgcagac
//
LOCUS       DASWPU010000264         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_63755,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000264 DASWPU010000000
VERSION     DASWPU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63755"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..701
                     /locus_tag="VGC27_05665"
     CDS             519..701
                     /locus_tag="VGC27_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282092.1"
                     /translation="MAMSRFKRGVSLRGSRSNVIAASCAAKNAPFSQAASARTSCAFE
                     HFFFIPELYGRAAEAG"
     gene            942..1358
                     /locus_tag="VGC27_05670"
     CDS             942..1358
                     /locus_tag="VGC27_05670"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEY0282093.1"
                     /translation="MCSRRLIADPNRRRSLKQGLASELRNSATDAERKLWSKLRSKQF
                     AGLRFRRQQPIGPYIADFYCSAAKLVVELDGDQHGDDRHVQYDAVRTRWLSTQGYRVL
                     RFPNGEFLRNPQAVLDGIWNALNDSGIPLPKPPSAV"
     gene            complement(1366..>1570)
                     /locus_tag="VGC27_05675"
     CDS             complement(1366..>1570)
                     /locus_tag="VGC27_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282094.1"
                     /translation="PVLIVGAGKDRIVRVEATRAFARRLPHGTYLELADAEHEILMEN
                     DSIRARFWQAFDEFVGAALPPSP"
BASE COUNT          282 a          487 c          508 g          293 t
ORIGIN      
        1 cgactgggag ggaaacccgt ggcgcgacgc cggcccggcg atcgcggcgg gcgacgcgcg
       61 cgtccatgcg caggcgctga agctccttca gggctaagac tctttttggt cgcgcgctct
      121 tgcggcggtc gcgtccgcat gccgtcgctg cggaccgctt cgcctgagcg cgctctatgg
      181 cgatttcgct ttcggccaag gccagcgcgg cgcggacttg cctctcgaac gctttgaggc
      241 gggcagacag ggcgcggacc tctttggtgt agcgcccgct gcggacggca ttgcggttgc
      301 ccgagggcgc tccacagcgc caggcgggcg ctccacagcg ccatttgcca gggattgcct
      361 ggggtgttgt ttcgaaaata acgagagagg ggtacccccc ggcgccctcc tggcttcgag
      421 gagcaaatcc ccttccgacc ctgtaacccg cgcactccaa ccgcgccaag atcaccgcaa
      481 gaccggccgc cagcgacagg actttcgcgg cggcgcggat ggcgatgtcg cggttcaagc
      541 gcggcgtctc gttgcgcggt tcgcgcagca atgtgatcgc ggcctcgtgc gcggcgaaga
      601 atgcgccgtt cagccaagcg gccagcgcgc gcacatcttg cgcgttcgag cacttcttct
      661 tcatccccga attatacggc cgcgcggccg aagccggata agttcaagcc tgtgccggta
      721 aggtgttgag gaagcaaacc ggtttgccgt ttcacgaatt ggataagctc acacgccaat
      781 ttatcgtggc ccatagaagg ctcaaggtct cccggttgaa aaagggcgcg gagcggagtc
      841 actctaattc taccctcccc ttgagggagg gtcgatccgg cgaagttgtc ccgagcgaaa
      901 gcgagggact tgtgaaagcg gatcggggag gggtcccggc gatgtgttcg agacgattga
      961 ttgctgaccc aaaccgtcgc cggtcgctga aacagggtct tgcgtctgag cttcggaatt
     1021 cggcgacgga cgcggagcgc aaattgtggt ccaagctgcg cagcaagcaa tttgccggac
     1081 tgcgcttccg tcgccagcaa cctattggcc cttatatcgc ggacttctat tgctcagcgg
     1141 ccaagttggt ggtcgaactc gacggtgatc agcacggcga tgatcggcat gtccaatacg
     1201 acgccgttcg aactcgctgg ctgtcgacgc aaggctatcg tgtactgcgc ttcccgaacg
     1261 gcgaattctt gagaaatccg caggccgtgc tcgacggcat ctggaacgct ctgaacgaca
     1321 gcggcatacc cctccccaaa ccgccttcgg cggtttgacc ctccctcaag gggagggtgg
     1381 aagtgcagca cccacgaatt catcgaacgc ctgccagaac cgcgcgcgga tggagtcatt
     1441 ttccatcagg atttcgtgtt cggcatcggc gagttccaga taggtcccgt gcggcaggcg
     1501 gcgggcgaaa gcgcgtgtcg cttcgacgcg cacgatgcgg tccttgcccg cgccgacgat
     1561 gagcaccggc
//
LOCUS       DASWPU010000265         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_20953,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000265 DASWPU010000000
VERSION     DASWPU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20953"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VGC27_05680"
     CDS             <1..626
                     /locus_tag="VGC27_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282095.1"
                     /translation="FDAAPFVAACGGNGTSWSTITNAYCIGNGGENVTWTFTASKSVK
                     GAPLYGTEINWQQQLDFLPHPFDSMGILANFTYVQAQQTYLNSTGTGVLMTADLTNMS
                     RNSYNATLYYDDTVFQARVTAAFRSHYLIDPNVLSNYNNYGIFVKSTFNLDASSSYRL
                     DEHLMFTVDVLNLTNQASNVYADAFAQRTYQYHETGRVFYLGVKYTY"
     gene            853..>1623
                     /locus_tag="VGC27_05685"
     CDS             853..>1623
                     /locus_tag="VGC27_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY0282096.1"
                     /translation="MRKVFAGLRTLRGALVVSLAILAVGASASAVFAATQDDTGINNG
                     LLFSLSAGKGLKADFAKGDAAPSFNDKVSAVQDPKSGPYIRSEGEQVLAWRAPSNIYA
                     QRGTLSFRWRARDPLGAQPFPLFRVSYADHTSWDMVWLRIDWNGNGLDAFVTDNNLAR
                     VRVSARMDAIPAPGHWLNIALCWDETQGIRLYVDGKLAARKDAAAVLDSGLDQFGPFS
                     RIISPYQVQSSYQFVRGGDVADIRIFDHALAPKSIAALA"
BASE COUNT          310 a          498 c          483 g          332 t
ORIGIN      
        1 gatttgacgc cgctccgttt gtcgcggcgt gcggcggcaa cggcacgagc tggtccacca
       61 tcaccaatgc gtactgcatc ggcaatggcg gcgagaacgt gacctggacg tttacggcgt
      121 cgaagagcgt caagggtgct ccgttgtacg gcacggaaat caattggcag cagcaactcg
      181 actttctgcc gcatccgttc gatagcatgg gcattctggc caacttcacc tatgtccagg
      241 cgcagcagac ctatctgaat tcgaccggca ccggcgtctt gatgacggcg gatctgacaa
      301 acatgtcgcg caacagctat aatgcgacat tgtactacga cgatacggtc ttccaggcgc
      361 gtgtgaccgc ggcgttccgc agtcattacc tgatcgatcc gaacgtcctc tccaattaca
      421 acaactacgg catcttcgtg aagtcgacgt tcaacttgga tgcatcctcc tcgtacaggt
      481 tggacgaaca cctcatgttc acggtcgatg tgctgaatct gaccaatcag gccagcaacg
      541 tctatgcgga cgcgttcgcc cagaggacct atcagtatca cgagaccggc cgggtgtttt
      601 accttggcgt gaagtatacc tactaagcag ttggtaccta cccgtccgat ttgcattgct
      661 tggcgccggg gacaacctcc ccggcgctat tttttagcga gtggcgcgcg cctcttggcg
      721 atgacaacga aggacggcat cgctatggtc gcagtcgagg aaaccgtgat gaagggcagg
      781 gagtgcgcct catccggttg gaaatatagc ggaccgtttg ggaagtccgc tcccaggtgc
      841 ggggagtaaa agatgaggaa ggtcttcgcc gggctgcgga ctcttcgcgg cgccttggtt
      901 gtttcgttgg caatcctggc agtgggcgca agcgcttccg cggtctttgc cgcgacccaa
      961 gatgacaccg gtatcaacaa cggactcctc ttctccctct ccgccggcaa ggggttgaag
     1021 gccgatttcg ccaagggcga tgcggccccc agcttcaacg ataaggtttc ggccgtccaa
     1081 gacccgaaat ccggtcccta tatccgttcc gaaggcgagc aggtgctggc ctggcgggca
     1141 ccgtccaaca tctacgccca gcgcggaacg ctgtcgttcc gctggcgcgc gcgcgatccg
     1201 cttggcgccc agccatttcc gctgttccgc gtcagttacg ccgaccacac cagttgggac
     1261 atggtgtggc tgcgcatcga ttggaacgga aacgggctcg atgccttcgt caccgataac
     1321 aatctggcgc gcgtgcgcgt ctccgcccgc atggacgcga tccccgcgcc cggccactgg
     1381 ctgaacatcg ccttgtgctg ggacgaaacg cagggcatcc gcctctatgt cgacggcaaa
     1441 ttggcggcgc ggaaggacgc ggctgccgtc ctcgatagcg gtctcgacca gttcgggccg
     1501 ttcagccgca tcatcagccc ttatcaggtg cagtcgagct atcagttcgt gcgcggcggc
     1561 gacgtcgccg atatccgcat cttcgaccat gcgctggcgc ccaagagcat tgcggcgctg
     1621 gcg
//
LOCUS       DASWPU010000266         3207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_100483,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000266 DASWPU010000000
VERSION     DASWPU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100483"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VGC27_05690"
     CDS             <1..195
                     /locus_tag="VGC27_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282097.1"
                     /translation="AMSAFMLYVAGFIILLGGLVYGAFLLHVPQTWIGVGALVMIGFG
                     VMSAVSHTKPRDPPSAPPNA"
     gene            complement(423..1547)
                     /gene="rlmN"
                     /locus_tag="VGC27_05695"
     CDS             complement(423..1547)
                     /gene="rlmN"
                     /locus_tag="VGC27_05695"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY0282098.1"
                     /translation="MSTSASLLGLSPAALNASLLAAGVPQKAANMRVRQLWNWIYVHG
                     ARDFAAMTNLAKDFRAEMDARLSLARPEVVTEQTSSDGTRKWLLRSGGQEFETVFIPE
                     PGRGTLCVSSQVGCTLNCRFCHTGTQKLVRNLTPGEIVGQVMVARDALGDWPSTAEGR
                     RITNVVMMGMGEPLYNFENVKAALAIVTDGDALALSKRRVTLSTAGVVPMIARAGNEI
                     GSALAISLHAVRDEVRDMLVPLNKKYPLKELLDACRAYPGVSNARRITFEYVMLKGVN
                     DSLAEARELVRRIAGIPAKINLIPFNPWPGAPYECSDWAQIEKFAEIVNRAGYASPVR
                     TPRGRDIMAACGQLKSESVKRRASERMAEMRSAELAKMPE"
     gene            complement(1748..2266)
                     /locus_tag="VGC27_05700"
     CDS             complement(1748..2266)
                     /locus_tag="VGC27_05700"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY0282099.1"
                     /translation="MILALAGGCFAASLAPASAADSKSTLLGVSKDWSAYQATTGDGK
                     VCYALSKPKTIEPTKVSRDPIYLLVSDWPGRKVKGEFEVVPGYPYKDGEPAIAQVGSV
                     KVELFTRNDGTSGSAWVKDPGDEDKLREAMRKGSTLTISGVSQRGTKTKDTYSLSGIS
                     AMLDKVHATCSK"
     gene            complement(2418..>3207)
                     /locus_tag="VGC27_05705"
     CDS             complement(2418..>3207)
                     /locus_tag="VGC27_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0282100.1"
                     /translation="RMIQMIPMTVNWIGLWTIIRRETARILRVPIQVFVAPWISALLF
                     IFIFGFVVGPRIASIGGHRYLEFVLPGILMMNVINAAFLQSTSQIYFQRFQRYVEETL
                     VSPLSYVEMIAGSLTVVILRSVATALGILVIAALFGMTSIHGIGEFLFWVVAVSVIFG
                     FLGIIVGLWANSFEQLTVLNVFFIAPLSFVGGVFNTVGMLPPMLRWLAYGNPFFYFIN
                     GLRHSMIGFSEGSEAFGVILTLGLCAALAVAVWRLFSVGYGLRE"
BASE COUNT          628 a         1022 c         1000 g          557 t
ORIGIN      
        1 gctatgtccg ccttcatgct ctacgttgcc ggtttcatta tcctgctcgg cggtctcgtc
       61 tatggcgcgt tcctgctcca cgtcccccag acttggatcg gcgtcggcgc gctcgtcatg
      121 atcggtttcg gggtcatgtc cgcggtgtcg cacaccaagc cccgcgatcc gccgagcgcc
      181 cccccaaacg catagcgcag cctatgaacg caggcatcct gcgcaagacc gacggcagga
      241 ttatctttcc cttcggccgc gtggacgtga tgcttgaagc agcgtgaggg agatacccct
      301 ccccgaaaat tgctgtgcaa atttcgaccc tccctcaacc cgtcttcgct gaagcttcga
      361 cgggtaaggc acgtggtccg gcgaagcctt ggcgcagccg gactcaaggg gagggtggaa
      421 ggttactccg gcatcttcgc caactccgcc gagcgcatct cggccatgcg ttcgctggcg
      481 cgccgtttca cgctctcgct cttgagctgg ccgcaggcgg ccatgatgtc gcgtccgcgc
      541 ggggtgcgca ccggcgaggc gtagccggcg cggttgacaa tctcggcgaa tttctcgatc
      601 tgcgcccagt cggagcattc gtaaggcgcg ccgggccagg gattgaacgg gatcaaattg
      661 atcttcgccg gaatgcccgc gatccgccgc accagttcgc gcgcctcggc cagggaatcg
      721 ttcacgcctt tcagcatgac gtattcaaag gtgatgcgcc gcgcgttcga cacgccggga
      781 taagcgcggc aggcgtcgag cagttccttg agcggatatt tcttgttgag cggcaccagc
      841 atgtcgcgca cctcgtcgcg caccgcgtgc agcgaaatcg ccagcgccga gccgatctca
      901 ttccccgccc gcgcgatcat cggcaccacg ccggcggtgg acagcgtgac gcggcgcttg
      961 gacagcgcca gcgcatcgcc gtcggtcacg atggccagcg ccgctttgac gttctcgaaa
     1021 ttgtagagcg gctcgcccat gcccatcatc accacattgg tgatacggcg gccttccgcc
     1081 gtgctgggcc aatcgcccag cgcgtcgcgc gccaccatca cctggccgac gatctcgccc
     1141 ggcgtcagat tgcgcaccag cttctgggta ccggtgtggc agaagcggca attcagggtg
     1201 cagccgacct ggctggaaac gcacagcgtg ccgcgccccg gctcggggat gaacacggtc
     1261 tcgaattcct ggccgcccga acgcagcagc catttgcgcg tgccgtcgga ggaggtttgc
     1321 tcggtcacga cctccggccg cgccagcgag aggcgcgcgt ccatctcggc gcggaaatcc
     1381 ttggcgaggt tggtcatcgc cgcgaagtcg cgcgcgccgt ggacgtaaat ccagttccac
     1441 aactggcgca cgcgcatgtt cgccgccttc tgcggcacgc cggccgcgag cagcgaggca
     1501 ttcagggcgg cgggagaaag gcccaggagg gatgcagagg tgctcatcac attcatattg
     1561 tcattcccgg acgagcgaag cgaggggacc cgcctacgct aaagtttcga cgggcgagtc
     1621 ttcacataaa gcggaaggga gcccaggtgg atggaccaag cttgatctgt ccacctgggt
     1681 ccccttccct cgcccgtctt cgctgcttcg cagcttcgac gtggctcgcc ggggatgaca
     1741 accgcgatta tttcgagcag gtggcatgca ccttgtcgag catggccgag atgccgctca
     1801 gcgaataggt atccttggtc ttggtgccgc gctgagagac gccgctgatc gtgagcgtgc
     1861 tgcccttgcg catcgcttcg cgcaacttgt cttcgtcgcc ggggtccttg acccaggccg
     1921 agccgctggt gccgtcgttg cgggtgaaca attcaacttt gaccgagccg acctgggcga
     1981 tcgcaggctc gccgtccttg tagggatagc cgggtacgac ttcgaattcg cccttcacct
     2041 tgcgcccagg ccaatcgctg accagcaggt agatcggatc gcgcgaaacc ttcgtcggtt
     2101 cgatcgtctt gggtttcgac agggcatagc agaccttgcc gtcgccggtc gtcgcctggt
     2161 atgcggacca gtccttcgac acgccgagaa gggtggactt cgagtccgcc gccgacgcgg
     2221 gcgcaaggct cgcggcgaag cagccgccag ccaatgcaag aatcatcgaa aatcgcgaaa
     2281 aacctggcat tagttctcgt ctcccaaacc aacacgccgc gctccggcga accggcgcgg
     2341 agaataaggg cacgggccgg tcgcgtccat ggcggcggcc gaagaatcgc ccggcaagtg
     2401 ttaatgcgga gccgcgacta ttcgcgcaaa ccgtagccta ccgaaaacag ccgccatacc
     2461 gcgaccgcca gggccgcaca cagccccagc gtgaggatga cgccgaaagc ttccgatcct
     2521 tcgctgaagc cgatcatcga atggcgcagc ccgtttatga aataaaagaa aggatttcca
     2581 taggcgagcc agcgcagcat gggcggcagc atgcccacgg tgttgaagac ccctccgacg
     2641 aaggacagag gcgcgatgaa gaagacgttg agcacggtga gctgctcgaa actgttcgcc
     2701 cataggccga cgataatgcc gagaaagccg aagatcaccg acacggccac cacccaaaac
     2761 agaaattcgc cgatcccatg gatggacgtc atgccgaaca gagcggcgat cacgaggatg
     2821 ccgagcgcgg tggcgaccga gcgcaggatc actaccgtca gcgaaccggc gatcatctcc
     2881 acataggaca agggcgaaac cagtgtttcc tcgacataac gctggaagcg ctggaaatag
     2941 atctgcgagg tggattgcag aaaggcggca ttgatgacgt tcatcatcag gatgcccggc
     3001 agcacgaact ccaggtagcg gtggccgccg atcgaggcga tgcgcggacc gaccacgaag
     3061 ccgaaaatga aaatgaacag cagcgcggaa atccacggcg cgacgaacac ctggatcggc
     3121 acgcgcagga tgcgcgccgt ctcccggcgg atgatggtcc aaaggccgat ccaattgacg
     3181 gtcatgggga tcatttggat catgcgg
//
LOCUS       DASWPU010000267         3304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_39429,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000267 DASWPU010000000
VERSION     DASWPU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39429"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VGC27_05710"
     CDS             <1..1044
                     /locus_tag="VGC27_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282101.1"
                     /translation="AELDRRSVELGEKLVTAEALPQAIAEKRGALLDAIGAAETARDQ
                     AADARSEAETRLAEADKAAKAADHALSAAREERAREEALLEGAQARIAELRDRIRDEL
                     EIAPEELAERAELKDDEELPPLEQAEKRVEKLKQEREQLGGVNLRAEEEAAEHEQRLG
                     TLIADRDDLVGAIERLRRGIQSLNREGRERLLASFEKVNANFQSLFAKLFGGGEAKLT
                     FTESEDPLEAGLEIYARPPGKRLQSLSLLSGGEQALTAIALIFGVFLVNSAPVCVLDE
                     VDAPLDDANVERFCNLLDEMTRITETRFLVITHHALTMSRMHRLFGVTMVERGISQLV
                     SVTLAEAERVAAE"
     gene            1056..1322
                     /locus_tag="VGC27_05715"
     CDS             1056..1322
                     /locus_tag="VGC27_05715"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY0282102.1"
                     /translation="MAKSHEVGYADAMTDLLSRITIRADQCHGRPCIRGMRIRVADIL
                     EMLSNGVQAEEILRDFPDLEPADIRACLAYAASLTGHPVVNAAE"
     gene            1823..2152
                     /locus_tag="VGC27_05720"
     CDS             1823..2152
                     /locus_tag="VGC27_05720"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY0282103.1"
                     /translation="MPPPVPDDLKRLGERIDEAERKRSRPMKPPPPNSWGIAFRFATE
                     MVSALLVGGGLGWSIDWAFDHWSHLQTRPWGMVVMFVLGAVAGIRNVTRAAKEINAEM
                     AGKDLEK"
     gene            2163..2909
                     /locus_tag="VGC27_05725"
     CDS             2163..2909
                     /locus_tag="VGC27_05725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004482.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY0282104.1"
                     /translation="MNPMEQFEVKSIVPLHIGPYDVSFTNQSLFMCLVVLAVTLFLTL
                     AMSRGRLVPSRTQSMAEMSYEFVAGMIRSTTGDEGLVFFPFVFAIFMFVLFSNFLGLI
                     PGAFTVTSQIVVTFALAALVMLTVIAVGFARHGIGFFKLFVPKAPLLLLILLVPIEII
                     SFLTRLVSLSVRLFANMLAGHTMLVVFASFVVALGAAGGILSALSIAPMFMIVFIGAL
                     EMLVAFLQAYVFAILTCIYLNEALHLHEQH"
     gene            2932..3156
                     /locus_tag="VGC27_05730"
     CDS             2932..3156
                     /locus_tag="VGC27_05730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY0282105.1"
                     /translation="MDLQAAKMIGAGLACIALAGAGNGIGIVFGSYLQGALRNPGAAS
                     SQTTNLLLGFALCEATGLYGLVIALIILFT"
BASE COUNT          582 a         1053 c         1028 g          641 t
ORIGIN      
        1 gccgagctgg accgccgctc cgtcgaactc ggcgaaaagc tggttacggc cgaagctctg
       61 ccgcaagcca ttgcggagaa gcgcggcgct ttgctcgacg ccatcggtgc ggccgaaacc
      121 gcgcgcgacc aagccgccga cgcgcgcagc gaagcggaaa ctcgcttggc cgaggcggac
      181 aaggccgcca aggcggcgga tcatgccttg tcggcggcgc gcgaagaacg cgcccgcgaa
      241 gaggctttgc tggaaggcgc ccaggcgcgc atcgccgaac tcagggaccg catccgcgac
      301 gaactggaaa tcgccccgga agagttggcc gaacgggccg agctcaagga cgacgaagag
      361 cttcctccgc tcgaacaggc cgagaagcgc gtcgaaaagc tcaagcagga gcgcgaacag
      421 ctcggcggcg tcaatctgcg cgccgaggaa gaggcggccg agcacgaaca gcgtctcggt
      481 acgctgatcg ccgaccgcga cgatctggtc ggcgccatcg aaaggctgcg ccgcggcatc
      541 cagagcctga accgcgaagg ccgcgagcgg ctgctggcct ccttcgagaa ggtcaacgcc
      601 aacttccaga gcctgttcgc caagctgttc ggcggcggcg aggccaagct cactttcacg
      661 gaatccgagg atccgctgga agccggtttg gagatctacg cccgcccgcc gggcaagcgg
      721 ctgcaatcgc tctcgctgct gtcgggcggc gagcaggctc taacggcgat cgcgctgatc
      781 ttcggcgtgt tcctggtcaa ttccgccccg gtatgcgtgc tagacgaagt ggatgcgccg
      841 ctggacgacg ccaacgtcga gcgcttctgc aacctcctcg acgaaatgac ccgcatcacc
      901 gagacgcgct tcctggtgat cacccaccac gccctgacca tgtcgcgcat gcaccggctg
      961 ttcggcgtca ccatggtgga gcgaggcatc agccagctcg tttcggtcac cctggccgaa
     1021 gcggagcgtg tggcggcgga gtaaacgaag cttgcatggc gaaaagccac gaagtcggct
     1081 atgctgacgc tatgaccgat ttattgtcgc gcatcaccat tcgagccgat cagtgccacg
     1141 gacgcccgtg tattcgcgga atgcgcattc gcgtggcgga tatcctggaa atgctgtcga
     1201 acggtgttca ggctgaagag atcctgcgcg attttccgga tctcgaaccg gcggatatcc
     1261 gggcctgttt ggcttatgcc gcaagcctga cgggccatcc agtcgtcaat gccgctgaat
     1321 gagaacacac ggtgaaattc ctagttgacg cccaacttcc accggaccta gcggtctggt
     1381 tgcgtgcaca aggtcaagag gccatttccc tacgcgaaat ctcgcttcag cacgcagatg
     1441 accggaccat ctgggcgcgc gcggcctccg aaggcgcaat tattgtcacg aaggacgaag
     1501 attttgctct tcttgcgacc tcgcaagagg gaccttcggt actatgggta cgcacaggca
     1561 acgtcgtgaa tcgcattctc ctggcgcgat ttgaggccac atgggctcag ctatgcgccc
     1621 atttcgagtc gggggcgcgg ctcgttgagt tgcgataggc cggcggcccc cgcccatttc
     1681 gccgcgccgg taattcggtc aatccttcca acgcgttgcg gcaatttgcg catgcttgac
     1741 ggttttttgc cctgccccta tggtccgccc gctttcgaac cgagccgcga agcgttcaac
     1801 ggcaaaacag ggagccggtt gcatgcctcc tcccgtaccc gatgatctca agcgtctggg
     1861 cgagcggatc gacgaggccg agcggaaacg ctcgcggccg atgaagcccc cgccgccgaa
     1921 ttcgtggggc atcgccttcc gttttgcgac ggaaatggtt tcggcgctcc tggtaggcgg
     1981 cggcttggga tggagcatcg actgggcctt cgaccattgg tcgcatttac aaacgcgccc
     2041 atgggggatg gttgtgatgt tcgtgctggg cgcggtggcg ggaatccgca acgtgacgcg
     2101 cgcggccaag gagatcaacg ccgagatggc gggaaaagat ttggagaaat agcaccgtgg
     2161 cgatgaaccc catggagcag ttcgaggtca agtcgatcgt gccgctgcac atcggccctt
     2221 acgacgtctc cttcaccaac cagtccctgt tcatgtgcct cgtggtcctg gcggtgacgc
     2281 tgttcctcac gctggccatg tcgcgcggcc ggctggtgcc gtcgcgcacc caatccatgg
     2341 ccgagatgtc ctacgaattc gtcgccggca tgatccgctc gaccacgggg gacgaagggc
     2401 tggtgttctt ccccttcgtc ttcgccatct tcatgttcgt gctgttcagc aatttcctcg
     2461 gcctgatccc cggcgccttc accgtgacca gccagatcgt cgtgaccttc gcgctggcgg
     2521 cgctggtcat gctgacggtg atcgccgtcg gcttcgcgcg ccacgggatc ggcttcttca
     2581 agctgttcgt gccgaaagcg ccgctcctgc ttctgatttt gctggtgccg atcgagatca
     2641 tctcgttcct gacgcgcctc gtcagcctct ccgtccgtct gttcgccaac atgctggcgg
     2701 gccacaccat gctcgtcgtg ttcgcgagtt tcgtggtcgc gctgggcgcc gcgggcggca
     2761 tcctctcggc gctgtccatc gcgccgatgt tcatgatcgt cttcatcggc gccttggaaa
     2821 tgctggtggc gtttctgcag gcctatgtct tcgcgatcct gacctgcatc tatctgaacg
     2881 aagccttgca cctccacgaa caacactgaa gtaacgaagg agcttttcaa tatggatctt
     2941 caagcagcca aaatgatcgg cgcggggctg gcctgcatcg cacttgccgg cgccggcaac
     3001 ggcatcggca tcgtgttcgg cagctatctg caaggcgcgc tgcgcaatcc gggcgccgct
     3061 tccagccaga ccaccaacct gctgctcggc ttcgcgctgt gcgaagcgac cggcctgtac
     3121 ggcctggtga tcgccctcat catcttgttc acctgatgcc ggccgagaca gccaccgcca
     3181 cgggaaccgt tgcgccgcac ggcgccgagt tccctccgtt caagacggag acgtttccga
     3241 gccagctttt ttggctggcg gtgacgttcg ccttcttgct cgtggtgatg tggcgcatcg
     3301 tcgg
//
LOCUS       DASWPU010000268         3207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_81931,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000268 DASWPU010000000
VERSION     DASWPU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81931"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VGC27_05735"
     CDS             complement(<1..921)
                     /locus_tag="VGC27_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HEY0282106.1"
                     /translation="MKERQRIVAVIQARMGSTRLPGKVLKPIAGEPLLWHIVHRLKKS
                     RLIEEIAVATSTNPLDDAIAEFGKTYGVRVIRGPEDDVLARFARAAELLDADIIVRVS
                     SDAPFVDADFIDHLLTAMIEQGGDYVLLEDGAVTAHEGVDPMTRRALDKLMMDAAADP
                     VAREHVTGYFKLHPDFVKIARAGAYPRLARASGRLTVDTPDDLAFVQAVHARLEAKAG
                     EASLSDLLLLLEREPSLRQINAHVRQKEIAPQGGIALIRCDGGGGYGFGHVKRMIGLA
                     RALRDREGIGAVFAVNGSKEALAPIRQAGFK"
     gene            complement(918..1790)
                     /locus_tag="VGC27_05740"
     CDS             complement(918..1790)
                     /locus_tag="VGC27_05740"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEY0282107.1"
                     /translation="MAASRPLFIAEASSNHGRDLSRALAFVDAAADAGCDAVKFQLFK
                     IDRMFAPEILSRSTKHAARRQWELPLGYLEPLAEHCSERQIAFSCTPFYLEAVEELRP
                     HVAFYKIASYELLVHDLLAACAAAGKPVMLSTGMATMSEILEAVATLKAAGAADITLL
                     HCVSAYPMPAREANLAAIAALRGATGCKVGWSDHSRRPAVIERAVHRWGASAVEFHLD
                     LDGMGAEYQAGHCWLPQEIAPVIARVREAFEADGTGFKEPQPAELADRDWRADPLDGM
                     RPLRHIRAIWEPAA"
     gene            1891..2727
                     /locus_tag="VGC27_05745"
     CDS             1891..2727
                     /locus_tag="VGC27_05745"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY0282108.1"
                     /translation="MSRTTKAFFVVSSGRSGTAMLHKALSASGSVEMHHEYMVHIVQP
                     LAVRRYLGLVDSAEAKRLLAETHLAAIRYSDKAHWGDSSNKLSWLIAELAELLPQAKF
                     VHLVRDGRKVAGSYFRKLADECYDDRSTAILRAHIGDPLGVPPPPPEKKYWWPRPRKE
                     NPIAGAFADFGQFERIAWHWAEVNRVILAALAALPPRQSLFVRLEDLRENPNALRELY
                     DFLSLPYRSEHFALFARPHNVNRPEDRLLDETQRAQFEAIAGDMLARLGYSSRAEYVV
                     NY"
     gene            2776..>3207
                     /locus_tag="VGC27_05750"
     CDS             2776..>3207
                     /locus_tag="VGC27_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282109.1"
                     /translation="MPDICEQCRSDALEAVYTPERTVRGITVHICSHCGLVQSLPRAD
                     RAPRAAPAVSSGADWGNVRYGKGFRTKAVMAAMARHADLAAPLSLLDVGSNRGSFARA
                     FLGAAPNASIVAVEPDERVAESCGGLERTQLIASRIEDAPLE"
BASE COUNT          543 a         1021 c         1070 g          573 t
ORIGIN      
        1 cttgaacccg gcttggcgga tcggcgccag cgcctccttg ctgccgttca cggcaaatac
       61 cgcgccgatg ccttcgcggt cgcgcagcgc acgcgccagc ccgatcatgc gtttgacgtg
      121 gccgaagccg tagccgccgc cgccgtcgca gcggatgagc gcgatcccgc cttgcggggc
      181 gatctccttc tggcggacat gggcgttgat ctgccgcagc gagggttcgc gttccagcag
      241 caagagcaga tcgctcagcg acgcttcgcc ggccttggct tccaggcgcg catgcacggc
      301 ctggacgaaa gcgagatcgt cgggcgtatc gacggtcagc cgcccggagg cgcgcgccag
      361 tctgggatag gctcccgccc gcgcgatctt cacgaaatcg ggatgcagtt tgaaatagcc
      421 ggtgacgtgt tcgcgcgcga ccggatcggc cgccgcgtcc atcatcagct tgtcgagcgc
      481 gcgccgcgtc atcggatcga cgccttcatg cgcggtgacc gcgccatctt cgagcaggac
      541 gtaatcgccg ccctgttcga tcatcgcggt cagaaggtgg tcgatgaagt ccgcatcgac
      601 gaaaggcgca tcggaagaca cgcggacgat gatgtcggcg tccaggagtt cggcggcgcg
      661 cgcgaaacgg gcgagaacgt cgtcctccgg tccgcggatg acgcgaacgc cgtaggtctt
      721 gccgaattcg gcgatggcgt cgtccagcgg attggtgctg gtggcgaccg cgatctcttc
      781 gatcaggcgc gatttcttga ggcgatggac gatgtgccat aaaagcggct cgccggcgat
      841 gggcttcagc acttttccgg gcaggcgcgt cgatcccatc cgcgcctgga tcaccgcgac
      901 gatgcgctga cgctctttca tgcggccggc tcccagatgg cgcggatgtg gcgcagcggg
      961 cgcatgccgt ccagcggatc ggcgcgccag tcgcggtcgg ccagttcggc gggctgcggc
     1021 tccttgaaac cggttccgtc ggcttcgaaa gcttcgcgca cgcgcgcgat gaccggcgcg
     1081 atttcctgcg gcagccagca atggccggcc tgatactcgg cacccatgcc atcgagatcg
     1141 aggtggaatt ccaccgcgct ggcgccccag cgatggaccg cgcgttcgat caccgccggc
     1201 cggcggctgt ggtccgacca gcccaccttg cagccggtcg ccccgcggag ggcggcgatg
     1261 gcggcaaggt tcgcttcgcg tgcgggcatc ggataggcgg agacgcaatg caagagcgtg
     1321 atgtcggcgg ccccggcggc cttgagggtg gcgacggctt cgaggatttc gctcatcgtc
     1381 gccatgccgg tggagagcat gaccggcttg cccgcggcgg cgcaggcggc cagcaggtcg
     1441 tgcaccagca actcgtaaga ggcgatcttg tagaaggcga catggggacg aagctcttct
     1501 acggcttcca gatagaaggg tgtgcaggag aacgctattt ggcgctccga acagtgttcg
     1561 gccagcggct ccagataacc gagcggcagc tcccactgcc gacgcgcggc atgtttggtg
     1621 ctgcggctca aaatctccgg cgcgaacatc cggtcgatct tgaaaagctg gaatttcacc
     1681 gcgtcgcagc cggcgtcggc ggccgcatcg acaaaagcca gggcgcggga caggtcgcgg
     1741 ccgtggttgc tggaggcctc ggcaatgaaa aggggccgcg aagcagccat gggtaaagcc
     1801 cggttttgtc cttaacgctt tctaaagcgg cgttcactaa aagagcgtta acgagggggc
     1861 cgcgatggcc gttttgcttg cggtcaagtc atgagccgga cgaccaaggc gtttttcgtg
     1921 gtttcgagcg ggcgttccgg caccgccatg ctgcataagg cgttgtcggc ctcgggcagc
     1981 gtcgaaatgc atcacgaata tatggtgcat atcgtccagc ctttggcggt gcgccgctat
     2041 ctgggcttgg tggactccgc cgaggcgaag cgcctgctcg ccgaaaccca cctcgccgcg
     2101 atccgttaca gcgacaaggc gcattggggc gattcgtcga acaaactgtc ctggctgatt
     2161 gcggaactcg cggaacttct gccgcaggcc aaattcgtcc atctggtgcg cgacggacgc
     2221 aaagtcgccg gctcctattt tcgcaagctc gccgacgaat gttacgacga ccgctccacc
     2281 gcaatcctgc gggcgcatat cggcgatccg ctcggcgtgc ctccgccgcc gccggagaaa
     2341 aaatattggt ggccgcggcc gcgcaaggag aatcccatcg ccggtgcgtt tgcggacttc
     2401 ggccagttcg agcgcatcgc ctggcattgg gcggaagtca atcgcgtgat cctcgcggcg
     2461 ctggcggcgc ttccgccccg ccaaagcctg ttcgtgcgcc tggaggattt gcgcgaaaat
     2521 cccaacgcct tgcgcgaact ttacgacttt cttagcctac cttatcgcag cgaacatttt
     2581 gcgctgttcg cccggcctca caacgtcaac cgccccgaag acaggctgct cgacgaaacc
     2641 cagcgcgcgc aattcgaagc gatcgcgggc gacatgctgg cgcgactggg ttattcctcg
     2701 cgcgcggagt atgtagtgaa ttattaacgc tacgtggttg aagatggtta ccaggtcctt
     2761 cctggacatc ttcaaatgcc ggatatttgc gaacagtgcc gcagcgacgc gctagaagcc
     2821 gtttacacgc ccgagcgcac ggtcagaggg atcaccgttc atatttgcag ccattgcggg
     2881 ctggttcaga gcctgccgcg cgccgaccgc gcgccccgcg cggcgcccgc cgtgtcgagc
     2941 ggcgccgatt ggggcaatgt gcgctacggc aagggcttcc gcaccaaggc ggtgatggcg
     3001 gcgatggcgc gccacgccga tctggccgcg cctctctcat tgctcgatgt cggctccaat
     3061 cgcggcagct tcgcgcgcgc cttcctgggc gcggcgccga atgcgagcat cgtcgcggtc
     3121 gagccggacg agcgggtggc cgagtcctgc ggcggtctcg aacggaccca actgatcgcc
     3181 tcgcgcatcg aggatgcgcc gctcgaa
//
LOCUS       DASWPU010000269         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_14535,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000269 DASWPU010000000
VERSION     DASWPU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14535"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGC27_05755"
     CDS             complement(<1..263)
                     /locus_tag="VGC27_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282110.1"
                     /translation="MADTRVQPDAKMMAVAGRLARFIETLDRTLPARLFASGKVAIVE
                     NFPPYLFEGPGAVSAWAKGMRAHLAEVTALRHGFGPARDYSRSG"
     gene            complement(346..422)
                     /locus_tag="VGC27_05760"
     tRNA            complement(346..422)
                     /locus_tag="VGC27_05760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(386..388),aa:Pro,seq:cgg)
     gene            639..713
                     /locus_tag="VGC27_05765"
     tRNA            639..713
                     /locus_tag="VGC27_05765"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:671..673,aa:Gln,seq:ttg)
     gene            874..1719
                     /locus_tag="VGC27_05770"
     CDS             874..1719
                     /locus_tag="VGC27_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282111.1"
                     /translation="MHAAAENPGDLPKGASAARTEMSEMSDSSSRGEPKKAVWKHQKE
                     GAKMGVEMWGWGMKLWAAMMVVVWTCGSAFAGETTCSPHSQPQDDFIACAMRETEQKS
                     ADPINRTCAAQFADQTQRRKCISDGYAQRTAAERAAGTKMGDWLQIKKTDGKGSQVVI
                     ETTATAIQDATIRLNLECSERAGFQMTIRGTSSLGTPPRTTAYILIDGRPPIEMSAIQ
                     LVPEIDFPALLMRHSEPIEREILKSKDIQIRYDTRYGGSSSYSFKFANLKAGEKNVMS
                     VCPAP"
     gene            complement(1980..>2338)
                     /locus_tag="VGC27_05775"
     CDS             complement(1980..>2338)
                     /locus_tag="VGC27_05775"
                     /inference="COORDINATES: protein motif:HMM:NF018351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HEY0282112.1"
                     /translation="HYHKDGEMRFFPNNPNQDAYYEPNSFGGPVEQPDLAEPPLKISG
                     DADRYNHRDGNDDFGQPRALFNLFDAGQKQRLFSNIADAMAGVPQAIVERQLALFDKV
                     HPDYGAGVRAALKAHS"
BASE COUNT          519 a          686 c          712 g          421 t
ORIGIN      
        1 ccgctgcggc tgtaatcgcg ggccgggccg aagccgtggc gcagcgccgt aacctccgcc
       61 aaatgcgccc gcatcccttt cgcccaggca ctgacggcgc cgggaccttc aaacagataa
      121 ggcgggaagt tctcgacgat cgccactttc ccgctggcga aaaggcgcgc cggcagggtg
      181 cggtccaggg tctcgataaa gcgcgcgagc cggcctgcaa ccgccatcat cttcgcgtcg
      241 ggctgtacac gcgtatcggc catgggcacc cgcctccctt tgggcctggc tcgccgagcc
      301 gaagcccgga gaatggcctg ccaggcgtag ccgcgcggtg tagactggtc ggagagagag
      361 gattcgaacc tccggccccc acgtcccgaa cgtggtgctc taccaggctg agctactctc
      421 cggacctgat cgcattttcc gcggcagttc gccggaaaat gctccctcgg gaggcgctct
      481 tataacgatg ccccctgccc gccgcaaccg gccgaattgc ccgcgttgaa aggcgcttgc
      541 tcaagcctta tggtccaccg tcgccgaagc atggcggact cacgtaactc gaacccgccg
      601 gagcttcggc gaaggcgggt ccgcggcatt tagcgtgttg gggcgtcgcc aagtggtaag
      661 gcaccggatt ttgattccgg cattcccagg ttcgaatcct ggcgccccag ccacccttcg
      721 ctgcttcgca gcttcgggtg gctaacgctg tatcgcgcta gtaattacgt acatcggcgg
      781 ggaagtgaga tatggcgaga ctagccttga tatagcggga ttttttgagt tgttgccgcg
      841 tcattgtgag atcggcggcg cgcaagaaat agtgtgcacg cggcggccga aaatccaggc
      901 gatctgccaa aaggagcgag cgcggcgcgg accgaaatgt ccgaaatgtc cgatagctcc
      961 tcccggggtg aaccaaaaaa ggccgtctgg aagcaccaaa aagagggcgc taaaatgggt
     1021 gttgagatgt ggggatgggg catgaaactc tgggcagcga tgatggtggt ggtgtggact
     1081 tgcgggagcg cttttgcggg cgaaacaacc tgtagtccgc actctcaacc ccaagatgat
     1141 ttcatcgcct gtgctatgcg cgagacggag cagaaaagcg cggacccgat caatcgcaca
     1201 tgtgcagcac agtttgctga tcagactcag cgccgtaaat gcatatccga tgggtatgcg
     1261 caaagaaccg ctgctgaacg ggcggcggga accaagatgg gagactggct ccagataaaa
     1321 aagacggatg gaaaaggatc gcaggtcgta atcgaaacca ccgcgaccgc aattcaagac
     1381 gccacgatta gattaaacct tgagtgctca gagcgtgctg ggtttcaaat gacgatccgc
     1441 ggaacatcgt cattgggtac tccaccgcgc acaaccgcct acatactaat tgatgggcgc
     1501 ccgccaattg aaatgagtgc gattcagctc gttccagaga tcgattttcc cgccctacta
     1561 atgcgacact cggagccaat tgagcgggaa atcctcaaaa gcaaagatat ccaaattaga
     1621 tacgatactc ggtatggtgg gagttcctcc tacagcttca agtttgcaaa cttgaaggca
     1681 ggcgagaaaa acgtcatgtc agtgtgcccg gcaccatagg gcgcccaccg atatacactc
     1741 acgcgggacg ctaaatggag cccaagcgcc agcgcacccg gccctccctt tgagcaacaa
     1801 gaaagcgagg ccgccggccc tgccggagtc aagaaacctg gagcggaaaa ggggcggcgg
     1861 ccgcaatttc ccgagaaaaa ttatgcctcg gggacgcgac ggcaaaagtg tcggaatcta
     1921 cccatgcgac gaaaaatcct gcgcccggcc tggccgggcg ccggagaaag caattgcatc
     1981 tacgaatgcg ccttcagcgc cgcccgcacg cccgcgccat agtcgggatg cactttatcg
     2041 aacagcgcca gttggcgctc gacgatcgcc tgcggaacgc ccgccatcgc atccgcgatg
     2101 ttcgagaaca agcgctgctt ctggcccgcg tcgaagaggt tgaacagggc gcgcggctgg
     2161 ccgaaatcgt cgttgccgtc gcgatggttg tagcggtcgg cgtcgcccga aatcttcaag
     2221 ggcggctccg caagatcggg ctgctcgacc ggcccgccga acgaattggg ctcgtaatag
     2281 gcgtcctgat tcggattgtt ggggaagaac cgcatctccc catccttgtg ataatggt
//
LOCUS       DASWPU010000270         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_26611,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000270 DASWPU010000000
VERSION     DASWPU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26611"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1309
                     /locus_tag="VGC27_05780"
     CDS             158..1309
                     /locus_tag="VGC27_05780"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEY0282113.1"
                     /translation="MIPRTAISRAGLAAAALATLTLAGSASAAATPHIERAPFGRTPD
                     GQSVEIFTLTNKNGLEARIMTYGGKVVSLKTRDRDGRLQNIVRGSDTLDAYLAAMSYS
                     GSLIGRFANRIAGGRFSLDGVEYQLEKNSGSNNLHGGRQGFDKRVWSAHAFETEQGPS
                     LRLTYVSADGEEGFPGQLTVKVTYQLRNDDALSIDYRATTTKPTVVNLTNHAYFNLTG
                     DMDRDILAHMLTINADRYTPVDAALIPTGVEPVAETPFDFREPTAIGARIADDNEQLR
                     LTRGYDHNWVLMKPKPDALTTAAIVCDPASGRVLEVKTTEPGVQFYTGSFASGKTAGN
                     GPASRSRSGFALETQHFPDSPNQPSFPSTELRPGQTFHSQTVFIFRTMK"
     gene            1328..2155
                     /locus_tag="VGC27_05785"
     CDS             1328..2155
                     /locus_tag="VGC27_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEY0282114.1"
                     /translation="MDKFGLSRREILLAGGAALAATGSASARTPLTAADVIARIKANV
                     GIPWSDQTVDGIIAGDPMTPVKGIATTMMATFDALKAAVAAGKNMVITHEPTFWSHQD
                     DVSKLQAYPLYLQKLAYIKRNNLVSFHFHDHWHGLKPLDGVAVGMMRHLGWSHYVDAQ
                     NPRQFTLPRTTLLDLAREMRTKLGDRTMRVVGDPQLPVARVLASWGYVSPFPGIPQFN
                     GDEDVFVIGETREWELVEYAQDLIATGKKKGLVIVGHVLSEQWGMKYCAEWLGPIVP"
BASE COUNT          490 a          735 c          716 g          397 t
ORIGIN      
        1 tcatcaacgg ccagctcatg gcggtgctga tcgacgacga cccccaatcg atcaatcagc
       61 tttccggcct gttcggactg gagatcgaag ccaacaccaa agtctccgcc cgcaatatct
      121 atgtgaagaa gctcaattag ggcgggagga aaccgacatg ataccaagga ctgccatttc
      181 gcgggccggt ctggccgcag ccgcgctcgc gacgctgacg ctggcgggat cggcgagcgc
      241 ggccgcgacg cctcacatcg agcgggcgcc gttcggacgg acgccggacg gacagagcgt
      301 ggaaatattc acgctcacca acaagaacgg cctcgaagcg cgcatcatga cttacggcgg
      361 caaggtcgtc tcgctgaaga cgcgcgaccg ggatgggcgt ttgcagaata tcgtgcgcgg
      421 ctccgacacg ctcgatgcgt acctcgccgc catgtcctat tccggctccc tcatcgggcg
      481 cttcgccaac cggattgccg gcgggcggtt cagcttggac ggcgttgagt atcaactgga
      541 gaagaactcc ggctccaaca acctgcatgg cggacgccag ggcttcgaca aacgagtctg
      601 gagcgcccat gccttcgaga ccgagcaagg cccgtccctc aggctcacct atgtcagcgc
      661 cgacggcgaa gaaggctttc ccggccagct tacggtcaag gtcacttacc agttgcgaaa
      721 cgacgacgcg ctcagcatcg actaccgggc gaccaccacc aagccgacgg tcgtcaatct
      781 gacgaaccac gcctatttca atctgacggg cgatatggac cgcgacatcc tcgctcacat
      841 gctgacgatc aacgcggacc gctacacgcc ggtggacgcg gcgctgatcc ccacgggcgt
      901 ggagccggtc gccgaaaccc cgttcgattt ccgcgagccg acggccatcg gcgcgcgcat
      961 cgccgacgac aacgaacaac tgcggctcac tcgcggctac gaccataatt gggtgctgat
     1021 gaaaccgaaa ccggatgcgt tgacgaccgc ggctattgtc tgcgatcccg caagcggacg
     1081 cgtgctcgaa gtgaagacga cagagccggg cgtgcagttc tataccggga gtttcgcgag
     1141 cggaaagacg gcgggcaacg ggcccgcctc caggtcgcgg agcggattcg ccctggagac
     1201 gcagcatttc ccggactcgc ccaaccagcc gtctttccct tcgacggagc ttcgcccggg
     1261 ccagaccttc cactcgcaga cggtgttcat ttttcgtacg atgaaatagg cgaacaggga
     1321 gaggaccatg gacaagttcg gattatcgcg ccgcgagatt ctgctggccg gcggggcggc
     1381 gctggcggcg acaggttccg cttcggcgcg gacaccgctg accgccgcgg acgtgatcgc
     1441 gcggatcaag gccaatgtcg gcattccatg gagtgaccag accgtggacg ggatcatcgc
     1501 gggcgatccc atgacgcccg tgaaaggcat cgccaccacg atgatggcga cgttcgacgc
     1561 gctcaaggcg gcggtcgcgg cgggcaagaa catggtgatc acccacgaac cgacattctg
     1621 gtcgcaccag gacgatgtat ccaaattgca ggcctatccg ctctatttgc agaagctcgc
     1681 ttacatcaaa cggaacaatc tggtctcgtt tcatttccac gatcactggc acggcctcaa
     1741 accgctcgac ggcgtcgcgg tcggcatgat gcggcatctg ggctggagcc attatgtgga
     1801 cgcccagaat ccgcggcaat tcacccttcc ccggacgacg ctcctggact tggcgcgcga
     1861 gatgcggacg aagctgggcg accgcaccat gcgcgtcgtg ggcgatccgc aactgcccgt
     1921 cgcgcgcgtg ctcgcgagct ggggctatgt gagcccgttc cccggcattc cgcaattcaa
     1981 cggcgacgaa gacgtcttcg tcatcggcga aacccgcgag tgggagctgg tggaatacgc
     2041 ccaggacctt atcgccaccg gcaagaagaa aggcctcgtc atagtcgggc acgttctatc
     2101 ggaacagtgg ggcatgaaat actgcgccga atggctgggg ccgatcgttc cctaagtgcc
     2161 ggtggaattc atgccgatcg tcgaaccctt ctggaatccc gaccatccgg tgatggagat
     2221 caacacgaaa gtctagagcg caatccggca aagtggtcgg tagcagcagc gtgccgacgc
     2281 ggtttgccgt cagattgcgc gaaaatcaag gaatctagag cttgatccga ttcaatta
//
LOCUS       DASWPU010000271         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_33209,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000271 DASWPU010000000
VERSION     DASWPU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33209"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1122..>2744
                     /locus_tag="VGC27_05790"
     CDS             1122..>2744
                     /locus_tag="VGC27_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0282115.1"
                     /translation="MKRAGQTGAKYPGIPCVIHGNGAIAEVMGHVCGGVIGYPITPST
                     EISEIYEAFRAGGGCNVWGHHPFFFEPEGEHSAQSGALGAALTGGKYISNASSSQGIL
                     YGLESHYVTVGKRVGGFVLQVAARVVSKHSLNVMAGHDDVYALLQSGYTILFGSNSQE
                     AADLAAISYKISALSLIPVANAMDGFVTSHMLSEAVMPEPELLKEFLGDPSGRIKCPT
                     VAQEMLFGAKGRVFQLNQYLTRHQNDFVPGDLTKLKAFLDESADKIEKDNECALIEGT
                     LAWVPEELHSQWRRQWLNAYEKGTRQRVPALVDVHNPGLTGPVQNQPDFQAGSADHRT
                     HFVNAVPQFAREVMKEYSELTGREYGPIKTFKCDDAETVMIGLGSVTDDATAVATYLR
                     SQGKKVGVISVKLLQPFPEAEVAAALKGKKAVTVLERCDVTALTSFVTGALFKARENA
                     DGVRHPGIPALDKLPKITTAIFGLGAHDLQPRHLVAAFKNMETKNAPLVYLGSQFFSK
                     NPSPRMAELQAKLRAAYPETELMGLETETNPTL"
BASE COUNT          542 a          857 c          816 g          529 t
ORIGIN      
        1 agtgttcttc gtggcggccg tgcgggcggc catcctgctc ccacagggca taggcgcggg
       61 cctcgatctg tttgcgaagc gattccattg ccggcttggc gggcttgacg ttcatggcaa
      121 gactcccgtt tcggtttgtc acgagaaaca cacacgaatc gaggcgttgg atttcgcacg
      181 ggatcggcgg ccttgcaaga aatcggacca acgccgtctt aaaaacttca agttcacgga
      241 ctgaaaggcc gctctctttc tcttctggca cggcggatga acatgttcgc ggcactctgc
      301 tcattccaca tgcgttcttc gctccaaaag gtcttgtccg ggtgcggcaa acacgccgct
      361 cttgcgacgc gttcggatgt ccgcttgaag ggcggcgccc gcctggtgag cagcttggtt
      421 tcattcttgc ggatcgaaat aatcgttaca tttcaaatcg ttacttgctt gccagactaa
      481 cggaaaaagg cggcgcccgc ctcgtgctta accgtcgaac ggccgaaacc gatcggcttc
      541 ccctgcgtaa atacgtagca ctgcgcatag cgaggttgga tttgttggta tttcatcgcg
      601 gcgggtgccg tttgcaacgt aatccggcgc cagattgtct tcgggaaccg ccggaagcga
      661 gcgtctgccg tttgtcccgc gtccggcgat gcggagattg agacgagcgg cgtcaggcgc
      721 gacaccatgt gccacagaat ttgggattcc ccatcgtcta taatgcaagt gcggtcttgc
      781 aggctgactt cgaactggtg gtgccgcctt cggtttccgt tgcgatggtg gagtcgttcc
      841 cgcagccacg actttaacgc tgcgccttcg caaacggacg gaggccctgc ctgggccgca
      901 acactgaacg cgccgatgca tgttaccgac gcgcgtcaac acgtcctatc tgtaacccgg
      961 ccttcaggca tatttcagct tggaatctcc tcgccgaagg gccttaaagc cggcggaatg
     1021 atgatatcgg tcaatgcgac accctgggat gtcggttact gcaattgaaa tcgccatggg
     1081 gacacagcag tccccgggca aactggaggt agaagaggcg gatgaaacgg gcaggacaaa
     1141 cgggcgcgaa gtaccctgga attccttgcg taatccatgg taacggcgcc attgccgagg
     1201 tgatgggcca tgtctgcggc ggtgtcatcg ggtatccgat cacgccctcc acggaaatct
     1261 cggaaatcta cgaggcgttc cgcgccggcg gcggctgcaa tgtttggggc caccacccgt
     1321 tcttcttcga gccggaaggc gaacattccg cccagtcggg cgcgctcggc gcggccctga
     1381 ccggcggcaa gtacatttcc aacgcctcgt ccagccaagg catcctctac gggcttgaat
     1441 cgcattacgt caccgtcggc aagagagtcg gcggcttcgt gttgcaggtt gcggcccgcg
     1501 tcgtctccaa gcattcgctc aacgtcatgg ccggccacga cgacgtctac gccctgcttc
     1561 agtccggcta caccatcctg ttcggcagca attcgcagga agcggccgat ctcgccgcga
     1621 tctcctacaa gatcagcgct ctgtcgctga tcccggtcgc caacgccatg gacggcttcg
     1681 tcaccagcca catgctgtcc gaagcggtaa tgccagagcc ggaattgctg aaggaattcc
     1741 tcggcgatcc ctccggccgc atcaaatgcc ccacggtggc gcaggagatg ttgttcggcg
     1801 ccaagggccg ggtgttccag ttgaaccaat atctcacgcg gcaccagaac gatttcgtgc
     1861 cgggcgattt gaccaagctg aaggccttcc tcgacgagtc tgccgacaag atcgagaaag
     1921 acaacgaatg cgcgctgatc gagggcacgc tcgcatgggt tcccgaggaa ttgcacagcc
     1981 agtggcgccg ccaatggctg aacgcctacg aaaagggaac gcgccagcgc gttccggcgc
     2041 tggtcgacgt ccacaatccc ggcctgaccg gcccggtgca gaaccagccc gatttccagg
     2101 ccggctcggc cgaccaccgc acccatttcg tcaatgcggt gccgcagttc gcccgcgagg
     2161 tgatgaagga atattccgag ctgaccggcc gggaatacgg cccgatcaag accttcaagt
     2221 gcgacgacgc cgaaaccgtc atgatcgggc tgggctcggt caccgacgac gccacggcgg
     2281 tcgcgaccta tctgcgcagc cagggcaaga aagtcggcgt catctccgtc aagctgctgc
     2341 agccgttccc ggaagccgaa gtcgccgccg cgctcaaggg caagaaggcg gtcaccgtgc
     2401 tggagcgctg cgacgtcacc gcgctgacca gtttcgtcac cggagcgttg ttcaaggcgc
     2461 gcgagaatgc cgacggcgtt cgccatcccg gcattccggc gctggacaag ctgcccaaga
     2521 tcaccaccgc gatcttcggg ttgggcgcgc atgatttgca gccgcgccat ctggttgccg
     2581 cgttcaagaa catggaaacc aagaacgcgc cgctggtcta tctcggcagc cagttcttct
     2641 ccaagaaccc cagcccgcgc atggccgaac tccaggccaa actccgggca gcttatcccg
     2701 agaccgaact gatgggtctc gagaccgaaa ccaatccgac gctg
//
LOCUS       DASWPU010000272         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_14546,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000272 DASWPU010000000
VERSION     DASWPU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14546"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..571
                     /locus_tag="VGC27_05795"
     CDS             275..571
                     /locus_tag="VGC27_05795"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEY0282116.1"
                     /translation="MTGIEKGGSSEARRKRLLFRAQRRGFKEVDLIFGTFAARELAGL
                     DDTGLGQLEALLEAPDQEVFAWLKGEAPVPAAYDTPLFARLRALCRRKDPTWNV"
     gene            559..>1728
                     /locus_tag="VGC27_05800"
     CDS             559..>1728
                     /locus_tag="VGC27_05800"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282117.1"
                     /translation="MERVSYIARTEGRLTVTGVPEGYDVWLAAEAALRLKGLVLFVVP
                     DDLHAAGACAAARFFAPGVPVLSFPAWDCLPYDRVSPRPDIESTRLATLSALARRTKD
                     SGPALVVTTVNAVLQRVPPREAVLGASFAARVGDKVSHEKLALFLAHNGYVRTGTVRE
                     PGDFALRGGIVDLWPPGERPLRLDFFGETLDAVRSFDAETQLSSGEVAAIELLPAGEV
                     PLDGDSISRFRSGYVAAFGPAGDDPLYESVSAGRHAQGMEHWLPLFYGKLETLFDFAP
                     RALVMLGHYFDEAKAARLELIADYYETRRQFLKGDGAAKGALKSPPYKPLQPDRLYLT
                     AEEWNQALARFAVRDLSPFQAPESMASVDAGGKLGRDFAPERTQGRVNVFEGAAAH"
BASE COUNT          269 a          568 c          584 g          307 t
ORIGIN      
        1 cgacctgggc gatcagcttt tccagccggg ggccgacgcc cggcagggtg cgcgtctcgg
       61 cgaagagagg gaacagaatc gctggccgca tggtgggata aggataatca aaccgcgaca
      121 gttcgtcatc gtggcgcttc ctcacccttc gacgggctca aggtgagggg gcagcaaaac
      181 tcccctcatg ctgagcctgt cgaagcatga cgggcgtgat ttgcgtagcc ggtgccactg
      241 gtccctgtgt ctgctccgcg ctattccccc gcccatgacc ggcatcgaaa aaggtggctc
      301 ttcggaggcc cgccgtaaac ggctgttgtt ccgggcccag cgccggggct tcaaggaggt
      361 cgatttgatc ttcggaacct tcgcggcacg cgagctggcg gggctggacg ataccggcct
      421 cggccagctt gaagcgctgc tcgaggcgcc ggatcaggag gtttttgcct ggctaaaggg
      481 cgaggcgccg gtgcccgctg cctatgacac ccctctgttc gcgcggctgc gagctttatg
      541 caggcgtaaa gacccgacgt ggaacgtgta agctatatcg cccgcaccga gggccgcctc
      601 acggtcaccg gtgtgccgga gggttacgac gtctggctgg cggcggaagc ggccttgcgc
      661 ctgaaggggc tggtgctgtt cgtcgtcccg gacgatctgc acgccgccgg cgcctgcgct
      721 gcggcgcgct tcttcgcgcc cggcgtgccg gtattgtctt tcccggcctg ggattgcctg
      781 ccttacgacc gcgtctctcc ccggcccgac atcgaaagca cgcggctggc gacgctgtcg
      841 gccttggcgc ggcgcaccaa ggattccgga ccggcgctcg tcgtcaccac ggtcaacgcc
      901 gtcttgcagc gcgtgccgcc gcgcgaagcc gttctgggcg caagcttcgc cgcccgcgtg
      961 ggagacaaag tcagccacga aaagctggct ttattcctcg cccataacgg ctatgtgcgc
     1021 accgggacgg tgcgcgaacc gggcgatttc gcgctgcgcg gcggcatcgt cgatctgtgg
     1081 ccgccgggcg aacggccgct gcggctggac tttttcggcg aaacgctcga cgccgtcaga
     1141 agcttcgacg ccgagacgca attgtcgtcg ggtgaagtcg ccgccatcga gcttttgccg
     1201 gccggcgaag tgccgctcga cggcgattcg atcagccgct tccgcagcgg ctatgtcgcc
     1261 gccttcggcc cggccggcga cgatccgcta tacgaatccg tcagcgccgg gcgccacgcg
     1321 caaggcatgg agcattggct gccgctgttc tacggcaagc tcgaaacgct gttcgatttc
     1381 gcgccgcgtg cgctggtgat gctcggtcac tatttcgacg aggcgaaagc cgcgcggctg
     1441 gagctgatcg ccgattacta cgagacgcgg cgccaattcc tgaaaggcga cggcgcggcc
     1501 aagggcgcgc tgaaatcccc gccctacaaa ccgctccagc cggaccggct ttatctgacc
     1561 gccgaggaat ggaaccaagc gcttgcgcgc tttgcggtgc gcgatctttc gccgttccag
     1621 gcgccggaat ccatggcctc ggtggatgcg ggcggcaagc tgggccgcga cttcgcgccg
     1681 gagcgcacgc aaggccgcgt caatgtcttc gagggcgcgg ccgcacat
//
LOCUS       DASWPU010000273         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_2214, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000273 DASWPU010000000
VERSION     DASWPU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(706..894)
                     /locus_tag="VGC27_05805"
     CDS             complement(706..894)
                     /locus_tag="VGC27_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282118.1"
                     /translation="MFQITGDARNQTAKEPVTPQDLKTLILKLRWAGLDQDADKLCHE
                     LEQIAPEDCIPLCPAETD"
     gene            1155..>2595
                     /locus_tag="VGC27_05810"
     CDS             1155..>2595
                     /locus_tag="VGC27_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282119.1"
                     /translation="MKSIQRLAAVFTLAAAAACSPSIPPPPDTPKDATVDIVQGVEVA
                     DPYRWLENWADPKVQDWSDAQNKRTRSYLDALKNQEAVKDKLTKLVTATSPSYYGLSA
                     KGALVFAYYNDPQKQQPMIVALNEAADPASRKIVLDPNALDPSGHTAIDWFGASADGG
                     KIAVSLSKDGSEDGTLHIYDVASGNEIDAPIPNVQYPTAGGSLAWTADGTGFYYTRYP
                     GPDAPEDDQHFNLQVYFHKLGVAWNDDPLVLGKKDGLEKVSEIFLDNRFNLPTVMAMV
                     QRGDGNIWAFYVLKHGAEPIRIGTYGDEIVYASFGPDGAIYAISRKDSSNGKIVKLLA
                     PVQGGLATARVIVPESAVAILSGGAQQETPDLAFDRTHLFVRDIVGGPNQVRIFDLDG
                     KPAGKLPLPEIAANDEIVPLAGGEVLFNVSTYLRPRYFAAWNPVTGETRETALKVVSP
                     VSFADTEVTRVFATSKDGTKVPVNVVMKKG"
BASE COUNT          518 a          824 c          766 g          487 t
ORIGIN      
        1 gggccgggac ccgcccgccc aacatcagaa ccgaaaaaga tgaacccttg ctcaatcgcg
       61 ttcgctcaga ggaacgcgga atgcagtgca tcgaatagcg gataacaaag cgtgaagaag
      121 gttcctggcg tatccgccgc tcaccgtcca ccatccgccc taccctccgt gtctccagag
      181 aacacagtac gcaccggggc ttggtcggcg gccgcaacac gcggcgcacc gaggtcgcca
      241 tgttagcacg gatagtccga ttcccgacac aggcgatggg agcatccgcg tcagtgcggc
      301 gcgcacgccg tttacctagc ggcacacccc tgcgacattg gctggaagac cggatttcgg
      361 cggggatcgc gagcggcgca tagccggcgc ccgccaaatc tcgtggtccg cggacggccc
      421 gccacatcaa attgttttcg acttcacctt gaggggtggg gggtagtcta gcctttcctg
      481 ctccgcggac agtcaacttc atatatggga tatgggggcc caaaatggcg ggtcaatatc
      541 ccaaggagga ttctccgtgt tcgcggcgaa gacgcagctt ccgcccaggc aatgatgcag
      601 atgccccgca tggcgttcag ccccggccgc gcttcgcgca aatccagatg cgggtcatga
      661 catcgttcgg gttaacgggc gtgttacgct tgccctcttt tcgccctaat ccgtctccgc
      721 cgggcacaag gggatgcagt cctccggggc gatttgttca agctcgtggc agagcttgtc
      781 ggcgtcttgg tccaggccgg cccaacgcag cttgaggatg agagtcttga ggtcctgcgg
      841 cgttactggc tcttttgcag tttggtttct cgcgtcgcca gtaatttgaa acattcatac
      901 ctcctactac acacacttgt actttttctt tcttgccggt cttcttgcgc tcgcacatca
      961 gtattgattt gtactgttct atatcagaac tgatggtaac ggctattgga tcattgtggg
     1021 ggcaattgac gcagagcgtc ccgccgcgtc gcgatcacga tggcgcgaga gcactcgccg
     1081 gactcgccac attcgcagta tcctgacaag cgtacaaaaa accgcaccac gtcctcgctc
     1141 cgggagcatc gtccatgaaa agcatccagc gcctcgccgc cgtcttcacg ctcgctgccg
     1201 ccgcggcttg ttcaccctcg atcccgcctc cgcccgacac gccgaaggac gcgaccgtcg
     1261 atatcgtgca gggtgttgag gtcgcggacc cgtaccgctg gctggagaac tgggcagatc
     1321 ccaaggtgca ggactggagc gatgcccaga acaagcgcac ccggtcatat ctcgacgcgc
     1381 ttaaaaacca ggaagcggtc aaagacaagc tgacgaagct ggtcaccgcg acttcgcctt
     1441 cctattacgg attatccgcc aaaggggcac tggttttcgc ttactacaac gacccgcaga
     1501 agcagcagcc gatgatcgtg gcgctgaacg aagcggccga tccggccagc cgcaaaatcg
     1561 tgctcgatcc caatgcgctc gatcccagcg gccataccgc gatcgactgg ttcggggcct
     1621 ccgccgacgg cggcaagatc gcggtctccc tttccaagga cggaagcgag gacggaacgc
     1681 ttcacattta cgatgtagcc agtggcaatg agatcgatgc gccgatcccg aacgtgcaat
     1741 atccgacggc gggcggcagc ctggcctgga cggcggacgg aacaggcttc tactacacgc
     1801 gctatcccgg ccccgacgcg cccgaagacg atcagcattt caacctgcaa gtctatttcc
     1861 acaagctggg cgtggcatgg aacgacgatc cgctggtgct gggcaaaaaa gacggcctgg
     1921 aaaaggtttc cgagatattc ctcgacaatc gctttaacct tcccacggtc atggcgatgg
     1981 tccagcgcgg cgacggcaac atctgggcct tctatgtgct gaagcacggc gccgagccga
     2041 ttcgcatcgg cacctatggc gatgaaatcg tctatgcctc cttcgggccg gacggggcga
     2101 tctacgccat ctcgcgcaag gacagctcca acggcaagat cgtgaagctg ctggcgccgg
     2161 tgcaaggggg cctcgcgacc gccagggtga tcgtgccgga aagcgccgtc gccatcctgt
     2221 cgggcggtgc gcagcaagag acccccgacc tcgccttcga ccgcacgcac ctgttcgtgc
     2281 gcgacatcgt cggcgggccg aaccaggtcc ggattttcga tctcgacggc aagccggccg
     2341 gcaagctgcc cttgcccgag atcgccgcca acgacgagat cgtcccgctg gcaggcggcg
     2401 aggtcctgtt caacgtctcg acctatctgc gcccgcgtta tttcgcggca tggaacccgg
     2461 tgaccggcga gacgcgggag accgcgctga aggttgtctc gcccgtctcc ttcgccgata
     2521 ccgaagtcac gcgcgtgttc gccacctcga aagacggtac gaaggttccg gtcaacgtcg
     2581 tgatgaagaa gggca
//
LOCUS       DASWPU010000274         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_33229,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000274 DASWPU010000000
VERSION     DASWPU010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33229"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1100
                     /locus_tag="VGC27_05815"
     CDS             132..1100
                     /locus_tag="VGC27_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0282120.1"
                     /translation="MAAASLAFIHVRGHRSMEIYLPIAEMSVHWLVVLGMGFGVGFLS
                     GMFGIGGGFLLTPLLIFYGIPPGVAVATTASQISAVTFSGVLAHWRRRSVDLKMGAVM
                     LGGGLVGTVLGVFLFGVLRRMGQSEFSVSASYVILLGAVGGLMLNESIRTLRAAGNGT
                     SAFGRKPGQHNWIHGLPLKMRFRESRLYISAIPVVALGFVGGVLAAIMGVGGAFLMVP
                     VMIYLLKMPTNVVIGTSLFQVLFVTAAATVLHAADNYTVDIVLALFLILGGVFGVQYG
                     VRVGAKLRGEQLRFWLAVLVLAVALRLFFEMVATPADLYSIAAGAP"
     gene            1097..1846
                     /locus_tag="VGC27_05820"
     CDS             1097..1846
                     /locus_tag="VGC27_05820"
                     /inference="COORDINATES: protein motif:HMM:NF021148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEY0282121.1"
                     /translation="MRRWLPAACAALLLAGPAGAADDLVSGLSQDVVQISSTYSGTDL
                     TVFGAIERPTDLAKSDIVVLLRGPDATMTVRRKDRFAGIWINNARARLIGLPSYYFLA
                     GTRPLEDIAPADTLRRYDLGLDNLRPDTALSDGSILPYVRALVRAKVKSGLYTQSSTA
                     VEMLSPTLFRVRVPVPAAVPRGSYMVQVYLFRDGTVVSAQSTPLYVDQTGLERRLYDF
                     AHAWPWSYGFATVLMAVLLGWLSSLFFRRGL"
     gene            complement(2053..2880)
                     /locus_tag="VGC27_05825"
     CDS             complement(2053..2880)
                     /locus_tag="VGC27_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEY0282122.1"
                     /translation="MTRRDDLHISVNGETLLLDKSGAAVWPAEKTLVFADLHFEKGSA
                     YAKRGALLPPYDTRSTIKRMAEAIGRHKPLRVIALGDSFHDGEAADRLDPEERAMLAR
                     LAAGAEWIWVEGNHDPKPPAWIGGRVAAEIAFGGLVFRHVPAPCRPGPDQLPLMLRQA
                     HPSRRRFAAPQDEGLGNPLTLSQSKGEGTRPGQAGEVAGHLHPATVVRRGGMSVRRRC
                     FVSNGARLLLPSFGAYTGGLDVRDIAIASLFADGFCVYALGRERVYTVKHKPAKCGP"
BASE COUNT          487 a         1051 c          974 g          610 t
ORIGIN      
        1 cactgcccgt tgccccgtta cccttgccca actggatggt ccgctgttgc gggccaggac
       61 actaacagga gagagcagca cgaatccgaa gccgccatta ccggttgacc gcttgattcc
      121 aggcgcgcag attggccgct gcttcgctgg ccttcatcca cgttcgcggt caccgctcga
      181 tggaaatcta tctgcccatc gctgagatgt cggtccattg gctggtcgtc ctgggcatgg
      241 gcttcggcgt aggcttcctc tccggaatgt tcggcatcgg cggcggcttc ctgctcaccc
      301 cgctcttgat cttctacggc atcccgccgg gcgtcgcggt ggcgaccacg gccagccaga
      361 tttccgccgt caccttttcg ggcgtgctcg cccattggcg gcggcgcagc gtcgatctga
      421 agatgggcgc ggtgatgctc ggcggcggct tggtcggcac ggtcctgggc gtttttctgt
      481 tcggcgtcct gcgccggatg gggcagtcgg aattctcggt gtccgcgagc tatgtgatcc
      541 tgctgggcgc ggtcggcggc ctgatgctga acgaatcgat acgcaccttg cgcgcggccg
      601 gaaacggcac ctccgccttc ggccgcaaac ccggccagca taattggatt cacggcttgc
      661 cgctgaagat gcgcttccgc gagtcgcggc tgtacatcag cgcgattccg gtggtggcgc
      721 tcggcttcgt gggcggcgtg ctggcggcga tcatgggcgt gggcggcgcc ttcctgatgg
      781 ttccggtgat gatctacctg ctcaagatgc cgaccaacgt cgtcatcggc acctcgctgt
      841 tccaggtcct gttcgtcacc gccgccgcga ccgttctgca cgcggcggac aattacaccg
      901 tcgatatcgt gctggcgctg ttcctgatcc tgggcggggt gttcggcgtg caatatggcg
      961 tgcgggtcgg cgccaagctg cgcggcgagc aattgcgctt ctggctggcg gtgctggtgc
     1021 tggcggtggc gctgcggctg ttcttcgaaa tggtggcgac gccggccgac ctctacagca
     1081 tcgcggcagg tgcgccatga gacggtggct gccggcggcg tgcgccgcac ttctcctggc
     1141 cgggcccgcg ggtgccgcgg acgatctcgt ttccggcctg tcgcaggacg tggtgcagat
     1201 ttcctcgacc tacagcggca ccgacctcac cgtcttcggc gccatcgagc ggcctaccga
     1261 cctcgccaag agcgatatcg tcgtgctgct gcgcggaccc gacgccacca tgacggtgcg
     1321 gcgcaaggac cgcttcgccg gcatctggat caacaatgcg cgcgcgcggc tgatcggcct
     1381 gccgtcctat tacttcctgg ccggcacccg cccgctggaa gatatcgccc cggccgacac
     1441 gctgcgccgc tacgatctcg gcctcgacaa tctgcgcccc gacaccgcgc tgagcgacgg
     1501 cagcattctg ccttatgtcc gcgccctggt gcgcgccaag gttaagagtg ggctttatac
     1561 gcagagcagc accgccgtcg aaatgctgag tccgacgctg ttccgcgtgc gcgttccggt
     1621 tcccgccgcg gtgccgcgcg ggtcgtatat ggtgcaagtc tatctgttcc gcgacggcac
     1681 ggtggtcagc gcgcaatcga cgcccctcta tgtggaccag accggcctcg aacggcggct
     1741 ctacgacttc gcccacgcct ggccgtggtc ttacggcttc gcgaccgtgc tgatggcggt
     1801 tctgctgggc tggctctcgt cgctcttctt ccgccgcggg ctatgagttt ctaaattttc
     1861 gtgcaatcca gagatgcttt gaattgtgaa catccctatt cgtcatggcc gggcttgacc
     1921 cggccatcca gttttcaaag ctttcggaaa tgaacgtttg cgctggatgg ccgggcttga
     1981 cccggccatg acgaaaatag agagtcaatt tatcccaagt ctgcttctgc ggcgaatgcg
     2041 cgtaccctgc cgctatggac cgcacttcgc tggtttgtgc ttcacggtat agacccgctc
     2101 gcgccccagc gcgtaaacgc agaacccgtc cgcgaacagc gaggcaatcg cgatgtcgcg
     2161 cacgtcgagc ccgccggtgt aagcgccgaa cgaaggcagc agcaggcgcg cgccgttcga
     2221 cacaaagcag cgccgcctga cgctcatgcc gccccgcctc acgacggtcg ccggatgcag
     2281 atgccccgcc acttcgcccg cctggcccgg ccgcgtccct tcacccttcg actggctcag
     2341 ggtgagggga ttacccaatc cctcatcctg aggagccgcg aagcggcgtc tcgaaggatg
     2401 agcttgtcga agcatgagcg ggagctgatc cggtccgggg cggcatggcg caggcacatg
     2461 gcggaacacc agtccgccga acgcgatttc cgccgccacg cgcccgccga tccaggccgg
     2521 cggcttggga tcgtgattgc cttccaccca aatccactcc gcgcccgccg caaggcgcgc
     2581 cagcatcgct cgctcctcgg ggtccagacg atccgcagct tcgccatcat ggaaggaatc
     2641 gcccagcgcg atgacgcgca aaggcttatg tctcccaatg gcctccgcca tccgcttgat
     2701 cgtcgagcgc gtatcgtaag gcggcagcaa ggcgccgcgc ttggcataag ccgagccttt
     2761 ctcgaaatgc agatcggcga aaaccagcgt cttctccgcc ggccaaaccg ccgcgccgga
     2821 cttgtcgagc agcagcgttt cgccgttgac ggaaatgtgg aggtcgtcgc gccgagtcat
     2881 tgcacgaaca tataccaaaa acttcaccct ccccttgctt gtccgccgta gctttagcga
     2941 aagcggaagg gagggtgaaa aaagtccctc actccacccg tatcgcctcc gcgatcagcg
     3001 cctgttcggt ttcgcgcaag acgtcctcgc ccgcttcgcc gacgatcatt tcgcggccga
     3061 tttccaagag cgccggcacc gccagcggcg aaacgcggtc gagcttgcgc ttgacgatgc
     3121 gc
//
LOCUS       DASWPU010000275        10702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_70034,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000275 DASWPU010000000
VERSION     DASWPU010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10702
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70034"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VGC27_05830"
     CDS             complement(<1..874)
                     /locus_tag="VGC27_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0282123.1"
                     /translation="MPQSDDNVPRGHIPHTPSAEVVFVRRILVAALIVVLLLALWRVS
                     LVLILTFGGVLVAIALRNLSVPLGRVLRLPDRVSVLIATLGVGLVVVGFFDLFGTMAS
                     DQFAALISQIPGAVESARTWLNGTTIGREILSALENSTDAALRVLSALPLAGGILGVI
                     GEVILVIAVGIYFAADPHTYIAGTLRLVPPRRRERARQIFDAMGLALRKWLTGMTLDM
                     LLLGVMTGFGMWLIGVPLPMALAVLSGVAVFVPYIGPAIATVPGLLLALSVSPTLALY
                     AAIVYLIVLTIEGNI"
     gene            complement(958..1893)
                     /locus_tag="VGC27_05835"
     CDS             complement(958..1893)
                     /locus_tag="VGC27_05835"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008307606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY0282124.1"
                     /translation="MKLLAGNSNRPLAEAIALYLKLPLTKAVVRRFADMEIFVEIQEN
                     VRGEDIFVIQSTSFPANDNLMELLIIIDALRRASAKRITAVLPYFGYARQDRKVGPRT
                     PISAKLVANLITEAGAHRVLTVDLHAGQIQGFFDIPTDNLYAMPAIAKDIKDYLGAGD
                     HLMVVSPDVGGVVRARALAKRLGGDLAIVDKRRERAGESEVMNIIGDVEGRSCIIIDD
                     IVDSGGTICNAAEALIKAGAKDVCAYASHGVLSGGAVARVQASVLKSMVVTDSIQVTD
                     DVRKCTNIRRISIAPLIGEALRRINNEESVSSLFD"
     gene            complement(1999..2766)
                     /gene="pgeF"
                     /locus_tag="VGC27_05840"
     CDS             complement(1999..2766)
                     /gene="pgeF"
                     /locus_tag="VGC27_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY0282125.1"
                     /translation="MLILHAKSLSRPRLAHGFFGRRGGVSEGIYASLNCGPGSGDDRA
                     KVIENRRRATQALGAQARLLTLYQIHSAKAVTVTQPWETGESPQADGLATAVPGIALG
                     MLTADCAPVLLADVEAGVIGAAHAGWKGALDGIVESTVAAMETLGAARGRIAAAIGPC
                     ISQANYEVDGEFRGNFTRADSANARFFVPNCRPGYFRFDLESFAALRLEGAGVTNIEK
                     LSACTYARAGDFYSFRRATHSGEKDYGRELSAIMLTK"
     gene            complement(2899..3978)
                     /locus_tag="VGC27_05845"
     CDS             complement(2899..3978)
                     /locus_tag="VGC27_05845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0282126.1"
                     /translation="MNALAERIAALIAAEGPLSIAQFMTLCLHDPAGGAYGARDPIGR
                     DFITAPEISQAFGELCGLWVAQTWHDQGRPGRPRLVELGPGRGTLMADALRAIGAAAP
                     EFVTETEIVLVETSPVLQAIQRERLKLSAADISWCARFDESLADRPLFLVANEFFDCL
                     PLRQFVKTAQGWHERMVTLQDGALAFALAPQPANPSLIPPNRADAPIGGVYEVAPSAL
                     ALAEEIARAVAAQGGGALIVDYGYDEVGFGETLQAVAGHKFAELLAEPGGSDLSAHVD
                     FLALAQAARNGGAAAYGPVTQCNFLADLGLGPRAERLIVANPLEGRAIAAAIDRLVNP
                     KEMGALFKALAMAPKTAPPPPGFTT"
     gene            complement(3975..4898)
                     /gene="lgt"
                     /locus_tag="VGC27_05850"
     CDS             complement(3975..4898)
                     /gene="lgt"
                     /locus_tag="VGC27_05850"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY0282127.1"
                     /translation="MVEALLPYPQIDPVLIHFGPVAIRWYALSYIAGLLLGWWLIVKM
                     LRQASLWKNPPFKGKAPATADDIGDLFVWVVIGVIVGGRLGYDLFYGTIFCGIWTGRD
                     CGNLPWGYLENPLRLLAEWDGWVPRLLGMSFHGGLIGVAVAILLYCRNRKLDVLRVGD
                     LIAAAAPIGLFFGRIANFINGELWGKVTDSPLGMVFCNRTILETYGGCPAGMQPRYPS
                     QLFEAAAEGLLLFLVLQICFRTFRLHERPGLIIAIFFAGYGLARVFTEFFRDSESMIT
                     SWFSMGMALSLAMWAVSALFFWIALKKPARA"
     gene            5020..5286
                     /locus_tag="VGC27_05855"
     CDS             5020..5286
                     /locus_tag="VGC27_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY0282128.1"
                     /translation="MQTDNPFLYGLAKLFTDAAGAAQSLRTEIDTFVKQRLEKLVAEM
                     DFVPREEFEAVKAMAAKARSENERLEARIAALESAAAPPAPRAG"
     gene            5414..5917
                     /locus_tag="VGC27_05860"
     CDS             5414..5917
                     /locus_tag="VGC27_05860"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY0282129.1"
                     /translation="MTVTHQDESVSRHHPLDMLERTVEENGWAFERSGRDELNLSVAG
                     RWSDHHFSFTWREDLQSLHLSSAFDMRVNGEARLADVAALLMYVNARMWIGHFDLWPE
                     DGTIVFRHAMIFPDAYASASQCEALLNLALEACEHYYPAFQFVLWGGKTAEEAVAAAI
                     LECAGQA"
     gene            6020..6820
                     /gene="proC"
                     /locus_tag="VGC27_05865"
     CDS             6020..6820
                     /gene="proC"
                     /locus_tag="VGC27_05865"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY0282130.1"
                     /translation="MTPQPILIIGAGKMGSALIKGWLKNGLGPILAVEPNPSPALRKL
                     KGLKFVSAVEAVPLRRWRACVVALKPQILKTEALRLKPIAALGVPMISIAAGTGERFL
                     AKAWGAKARIVRAMPNTPGAIGRGITALHAARGASAKDRKLAETLLAALGQTVWVKNE
                     ALIDAATAVSGSGPAYVFALAEALAKAGEAAGLPRATAERMARATVAGAGALLDADTR
                     PAAELRRDVTSPGGTTEAALKILLADDGLSALMTRAVAAAKQRAEELG"
     gene            complement(6817..8199)
                     /locus_tag="VGC27_05870"
     CDS             complement(6817..8199)
                     /locus_tag="VGC27_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0282131.1"
                     /translation="MSPTARFENLKAVLSRLRPGRLLKRVMPRGLLSRSLIIIVAPML
                     LLQAIVSYVFFERDLDITTRRMARDVAADIALLVAQEDTYPSPLREELRALAAKQLRY
                     DLSLERGAHITPSSGRYRSTIDIALDEVIAQQIGEARHFETASLPGKHKMDISVEVHD
                     GVLHAIVARDRITVSAPDLFIVWMVGSAMILLTVAILFLRNQVRPIERLARAADAYGK
                     GRAVPDFKPYGATEVRRAAQAFITMRERIERHVQQRTEMLAGVSHDLKTPLTRIRLQL
                     AMMPSGPEAEALREDIAEMERMLDEYLDFARGEGGEKSQLADLGDLVRDAAGAWARAR
                     RGGEERLALETPSGLLLTVKINALRRCVTNLVDNAFKHGGRVRVSLELGARFAAIHVD
                     DDGPGIALDKREEALRPFHRLDEGRNLQSGGVGLGLAIARDIARAHGGGLLLSESPDG
                     GLRATIRLPV"
     gene            complement(8177..8875)
                     /locus_tag="VGC27_05875"
     CDS             complement(8177..8875)
                     /locus_tag="VGC27_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0282132.1"
                     /translation="MSTVLQDPHLLVVDDDERLRTLLQRYLSSNGFRVSAAGSAADAR
                     ALMKSMAFDLLILDVMMPGESGFDLTKSVRAGSSVPILMLTAKSEAEDRIRGLEHGAD
                     DYLAKPFEPRELLLRVSALLRRAAPPAEKALAEVRMGDCVYDPERGQLRRKGKPVHLT
                     SSESTLLQFFAANAGRSFSRADLCARLGVALERSIDVQVTRLRRKIEEDPKLPLYLQT
                     VRGVGYVLVPDRAV"
     gene            complement(8872..9282)
                     /locus_tag="VGC27_05880"
     CDS             complement(8872..9282)
                     /locus_tag="VGC27_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0282133.1"
                     /translation="MDLLFVAARDISAQGANPLAKARLGRAHARALHFIARNPGLSVA
                     ELLGFLKVTKQSLNRVLNDLLKNGLVERKPGMQDRRTRRLRLTAAGAVLADAIWEEQR
                     PLLVSAFTAAGQDAVNGFRKVLLGLGGGGRKEKE"
     gene            9359..10324
                     /locus_tag="VGC27_05885"
     CDS             9359..10324
                     /locus_tag="VGC27_05885"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEY0282134.1"
                     /translation="MLTQIGRLLGQGKGLEDCTMAEVIPFDDRDGSLWYDGKLVPWRE
                     AKTHVLTHGLHYASCVFEGERLYSGRIYKLKEHTERLFESARLLGMTIPYTEDDVNAA
                     CMAAVHAQGYTDGYVRPVVFRGSEMMAVSAQKTKVHVAVAAWPWPSYFDPKEKLKGIR
                     LDISDWRRPAPDTAPTKAKAAGLYMICTMSKHVAEAKGYADALMFDYRGLVAEATGAN
                     IVFVKDGTLHTPTPDCFLDGITRRSVLALARKRQIPVVERHIDAKELADFSEAFLAGT
                     AAEVTPVSEIGPYHFKPGEISEMLMHDYAEDVRRPVSVVDLSAAG"
     gene            complement(10408..>10702)
                     /locus_tag="VGC27_05890"
     CDS             complement(10408..>10702)
                     /locus_tag="VGC27_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0282135.1"
                     /translation="LVILAREFLVSGLREFLAGADVSVPVSFFAKIKTTIQMIAIGAM
                     ILGPLADKVVPGALAFAYIALWVAAALTVGTGYVYLRAGLAHARPHPENETSA"
BASE COUNT         1905 a         3429 c         3602 g         1766 t
ORIGIN      
        1 tgatattgcc ctcgatcgtc agcacgatca ggtaaacgat ggccgcataa agcgcgagcg
       61 tcgggctgac gctgagcgcc agcaacaggc ccggaacggt cgcgatcgcc gggccgatat
      121 agggcacgaa caccgctacg cccgacagta cggccagcgc catgggcaat ggcacgccga
      181 tcagccacat gccgaagccg gtcatcacgc ccaagagcag catgtccagg gtcatgccgg
      241 tcagccattt gcgcagcgcc aggcccatcg catcgaagat ctggcgcgcc cgctcgcggc
      301 ggcgcggcgg caccagcctg agcgtgccgg cgatgtaggt gtgcgggtcg gccgcgaaat
      361 aaatgccgac ggcgatgacc aggatcactt cgccgatgac gcccaggatg cctccggcca
      421 agggcaaggc gctcagaacc ctgagggccg cgtcggtcga gttctccaac gcgctcagta
      481 tctcgcgccc gatggtcgtt ccgttcagcc aggtccgggc ggattccacc gcgcccggaa
      541 tctggctgat cagggcggca aattggtcgc tggccatggt gccgaacagg tcaaagaaac
      601 cgacgacgac aaggccgacc cccaaggtgg cgatgagcac cgaaacccgg tcgggaaggc
      661 gcaagacgcg gcccagcggc accgagaggt tgcgcagcgc gatcgccacc agaacgccgc
      721 cgaaggtcag gatcagaacc agcgacaccc gccacaaggc cagcagcagc acgacgatca
      781 gggccgcgac caggatccgg cgcacgaaca cgacttccgc cgaaggtgtg tgcggaatat
      841 ggccgcgcgg gacgttgtcg tcggactggg gcatggcggc gctcgcgaat ctggcgagag
      901 gatacacggt tttggcccaa ccgcgcgcgt ttccggcgcg gccgtgcttt ttcggcctca
      961 gtcgaacagg ctcgatacgc tctcttcgtt gttgatgcgg cgcaaggctt cgccgatcag
     1021 gggcgcgatg gagatgcggc ggatgttggt gcatttgcgc acgtcgtcgg tcacctggat
     1081 cgaatcggtg accaccatcg acttcagcac cgaggcctgg acgcgcgcca ccgcgccgcc
     1141 cgacaagacg ccgtggctgg cataggcgca gacgtctttg gctccggcct tgatcagggc
     1201 ctcggccgca ttgcagatgg tgccgccgga gtcgacgatg tcgtcgatga tgatgcagga
     1261 ccgcccttcc acgtcgccaa tgatgttcat cacttccgac tcgccggcgc gttcgcggcg
     1321 cttgtcgacg atggcgaggt cgccgcccag ccttttcgcc agcgcgcggg cccgcaccac
     1381 gccgccgaca tccggggaga ccaccatcag atggtcgccg gcgcccaggt aatccttgat
     1441 gtccttggcg atcgccggca tggcgtagag attgtcggtc gggatatcga agaagccctg
     1501 gatctgtccg gcatgcagat cgacggtcag cacgcggtgg gcgccggcct cggtgatcag
     1561 attggccacc agcttggcgg agatcggcgt gcgcggaccg actttgcggt cctggcgggc
     1621 atagccgaaa taaggcagca ccgcggtgat gcgcttggcg ctggcgcggc gcagcgcgtc
     1681 gatgatgatc agcagctcca tcagattgtc gttggcgggg aagctggtcg attggatgac
     1741 gaaaatgtcc tcgccgcgga cgttctcctg gatttcgacg aagatttcca tgtcggcgaa
     1801 gcgccgtacc acggctttgg tcaagggcag cttgagatag agggcgatgg cctcggccag
     1861 cggacggttg gaatttcccg cgagcagctt catgccccgg gttgtttttg tctctcccat
     1921 ggcccgcgct caccccgttt ttgtgacgat taggtgaccg gcttttagca aggggagggg
     1981 ggtgggtaaa ccacctaatt atttcgtcaa catgatcgcc gaaagttcgc ggccgtaatc
     2041 cttttcgccg gaatgagtcg cgcggcggaa gctgtagaaa tcgcctgccc tggcgtaggt
     2101 gcaggccgat agcttctcga tattcgtcac tcccgcgcct tccagccgca gggcggcgaa
     2161 agattcaagg tcgaagcgga aatagccggg gcggcagttg ggaacgaaga accgcgcatt
     2221 ggccgaatcg gcgcgcgtga aattgccgcg gaattcgccg tccacttcat agttcgcttg
     2281 gctgatgcac ggaccgatgg cggcggcgat gcgcccgcgc gccgcgccca gcgtctccat
     2341 tgccgcgacc gtgctttcga caatcccgtc gagcgcgcct ttccagccgg catgggccgc
     2401 gccgatcacc ccggcttcga cgtccgccag cagcaccggc gcgcaatcgg cagtcagcat
     2461 gccgagcgca atccccggca ccgccgtcgc caagccgtcg gcctgcgggc tttcgccggt
     2521 ctcccaaggc tgcgtgacgg tgacggcctt ggcgctgtgg atttgataga gcgtgagaag
     2581 acgcgcctgc gcgcccagcg cttgcgtcgc gcggcggcga ttctcgatca ccttggcgcg
     2641 gtcgtcgccc gagccgggtc cgcaattgag cgaggcgtag atgccctcgg aaacgccgcc
     2701 ccgccggccg aaaaaaccat gggcgaggcg cggacgggag agactcttgg cgtgcagaat
     2761 gagcatgatg ctgccatacc acgaattgcc tccccttctt gagggggagg cggaaaattt
     2821 tgcgaagcaa atttttcggt ggggggtgat gtcgacaatg cccctccccg atccgccttc
     2881 cctcaagtgg agggtaagtt aggtggtaaa tcccggcggc ggcggggcgg ttttcggcgc
     2941 catcgccagc gctttgaaca gcgcgcccat ctctttcgga ttcaccagcc ggtcgatggc
     3001 ggcggcgatg gcgcgcccct ccaggggatt ggcgacgatc agccgttccg cacgaggacc
     3061 aagtcccagg tcggcgagga aattgcattg cgtcacgggg ccataggccg ccgccccgcc
     3121 gttccgcgcc gcctgcgcga gggcaagaaa atccacatgc gccgacaggt cgctgccgcc
     3181 cggctcggcc aaaagctcgg cgaacttatg cccggcgacg gcctggagcg tctcgccgaa
     3241 gcccacttcg tcatagccgt aatcgacgat cagggcgccg ccgccttgcg cggccacggc
     3301 gcgcgcgatc tcttcggcca gcgccagcgc ggacggcgcg acctcgtaca cgccgccgat
     3361 gggggcgtcg gcgcggttcg gcgggattag cgacggattg gcaggctggg gcgccagcgc
     3421 gaaggcgagc gcgccgtcct ggagcgttac catccgctcg tgccagccct gcgccgtctt
     3481 cacgaattga cgcagcggca ggcaatcgaa gaactcgttg gcgacgagga acagaggccg
     3541 gtcggcaagc gattcgtcga aacgcgcgca ccaggaaatg tcagcggcgg agagcttcag
     3601 cctttccctc tggatcgcct gcaggacggg gctggtttcc accaggacga tttccgtctc
     3661 ggtcacgaat tccggcgccg cggccccgat ggcgcgcaag gcgtccgcca tcagcgtgcc
     3721 gcggccggga ccgagttcga ccagccgcgg gcggccgggc cggccctgat cgtgccaagt
     3781 ctgcgccacc cacagcccgc aaagctcgcc gaaggcctgg ctgatttccg gcgcggtgat
     3841 gaagtcgcgt ccgatgggat cgcgcgcgcc gtaagcgccg ccggccggat cgtgcaggca
     3901 aagcgtcatg aactgggcga tcgagagcgg gccttccgcg gcgatgagcg cggcgatccg
     3961 ttccgcgagc gcgttcatgc gcgcgccggt ttcttgagcg cgatccagaa aaagagcgcg
     4021 gacaccgccc acatcgccag actcagcgcc atgcccatgc tgaaccagct ggtgatcatc
     4081 gattcggaat cgcggaagaa ctcggtgaag acccgcgcca ggccgtagcc ggcgaagaag
     4141 atggcgatga tgaggccggg gcgttcgtgc aggcggaaag tgcggaaaca aatctgcagg
     4201 acgaggaaca gcaacaggcc ctccgccgcc gcttcgaaca actggctggg atagcgtggc
     4261 tgcatccctg ccggacatcc gccgtaggtc tcgaggatgg tgcggttgca gaacaccatt
     4321 cccaagggcg agtccgtgac ttttccccac aattcgccgt tgatgaaatt ggcgatgcgc
     4381 ccgaaaaaca ggccgatggg cgcggccgcg gcgatcagat cgccgacgcg caggacgtcc
     4441 agcttgcggt tgcggcagta caggagaatc gccaccgcga cgccgatcag cccgccgtgg
     4501 aacgacatgc cgagcaggcg cggcacccag ccgtcccact ccgcgagcag gcgcagcgga
     4561 ttctccagat agccccaggg aaggttgccg cagtcgcggc cggtccagat gccgcagaag
     4621 atcgtgccgt agaacaggtc gtagcccagc cgcccgccta cgatgacgcc gatcacgacc
     4681 cagacgaaca gatcgccgat atcgtcggcg gtggcgggcg ccttgccctt gaagggtggg
     4741 ttcttccaca ggctcgcttg gcgcagcatc ttcacgatca gccaccagcc cagcagcagg
     4801 ccggcgatgt aggacagcgc gtaccagcgg atcgcgaccg gcccgaagtg aatcaggacc
     4861 ggatcgatct ggggataagg caggagtgct tcgaccatcg tggctaaaac gcgctgcttc
     4921 ggccgggcct gtcaaggcga atagcgaatg gtaaacacgc tcccttccct attcgccgct
     4981 cgcttgccat ataaggaccg accacaagga cgaccgccca tgcaaaccga caatccgttc
     5041 ctctacgggc tggcgaaatt gttcaccgac gccgccggcg ccgcgcaaag cctgcgcacc
     5101 gagatcgaca cttttgtgaa gcagcgcctg gagaagctgg tcgccgagat ggatttcgtc
     5161 ccccgcgagg aattcgaagc ggtgaaggcg atggcggcca aggcccgcag cgagaacgaa
     5221 cggctcgaag cacgcatcgc cgcgttggag tcggccgccg ccccgcccgc tccgcgcgcc
     5281 ggatgaaacc tgtgaattgc cctttgcgtc catagggcaa ccgattgtga ctcttgtcac
     5341 cccacaaaat cttgtgtcag gttgacacgc aactgcttcg ttttgcgtcg cgcgccggtt
     5401 ccaggagtcg tacatgactg ttacccacca ggacgagtcc gtcagccgcc atcatccgct
     5461 cgatatgctg gagcggacgg tggaggagaa tggctgggcg ttcgagcgtt cgggccgcga
     5521 cgagttgaat ctgtccgtcg cggggcgctg gtcggaccat catttcagct ttacctggcg
     5581 tgaagatctg caatcgctgc atctgtccag cgccttcgac atgcgggtga acggcgaggc
     5641 gcggctggcg gatgtggcgg cgctgctgat gtatgtgaac gcgcggatgt ggatcggcca
     5701 tttcgatctc tggccggaag acggcaccat cgtcttccgt cacgcgatga ttttcccgga
     5761 tgcctacgcc agcgcctcgc aatgcgaagc gctgctcaac ttggcgctgg aagcctgcga
     5821 acattattac ccggcgtttc agttcgttct gtggggcggc aagacggccg aagaggcggt
     5881 ggccgcggcc atcctggagt gcgcgggaca agcgtgacgt aagcgcgtat gccttccttt
     5941 aacctccccc ttgtggggag gtcggcgacc ccggacttga tccgggggag ccgggtgggg
     6001 ggtagagtta ggacacgcaa tgactcccca accaattctg attatcggcg ccggcaagat
     6061 gggctcggcg ctgatcaagg gctggcttaa gaacggcctt ggtccgatcc tggccgtcga
     6121 gcccaatccg tcgccggctt tgcgcaagct gaagggtctg aaattcgttt ccgccgtcga
     6181 ggcggttccg ctgcggcgct ggcgcgcctg cgtcgtcgcg ctgaagccgc aaatcctgaa
     6241 aaccgaagcc ttgcgcttaa aacccatcgc agcgttgggg gtgccgatga tctccatcgc
     6301 ggccggcacc ggcgaaaggt tcctcgccaa ggcttggggc gcgaaggccc gcatcgtccg
     6361 cgccatgccg aacacgcccg gcgccatcgg ccgcgggatc acggcgctcc atgccgcccg
     6421 gggagcgagc gcgaaggaca gaaagctcgc cgagacgctg ctggcggcgc tcggtcaaac
     6481 cgtgtgggta aaaaacgaag cgctgatcga cgcggcgacg gcggtttcgg gctccggccc
     6541 ggcttatgtc ttcgccttgg cggaagcgct ggccaaggcg ggcgaagcgg ccggcctgcc
     6601 gcgcgccacc gccgaacgga tggcgcgggc gacggttgcg ggcgcgggcg cgctgctcga
     6661 tgccgacacc cgtcccgccg ccgagctgcg ccgcgacgtt accagtccgg gcggcactac
     6721 cgaagccgcg ctgaagattc tgctggcgga cgacgggctg tccgcgctga tgacgcgcgc
     6781 ggtcgcggcg gcgaaacagc gggcggaaga gctgggctaa accggcaatc tgatcgtcgc
     6841 gcgcaggccg ccgtccgggc tttcggacag cagcaacccg ccgccatggg cgcgggcgat
     6901 gtcgcgggcg atggccagtc ccagccccac cccgccgctc tgcagattgc ggccttcgtc
     6961 gaggcggtgg aacggacgga gcgcctcctc gcgcttgtcg agggcgatgc ccggcccgtc
     7021 gtcgtcgaca tggattgcgg cgaagcgcgc tcccaattcg agcgagacgc gaacccgtcc
     7081 gccgtgcttg aacgcgttat cgaccagatt ggtgacgcag cggcgcagcg cgttgatctt
     7141 caccgtcagc agcaggccgc tcggcgtttc gagcgcgagg cgctcctcac cgccgcggcg
     7201 ggcgcgggcc caggcgccgg cggcgtcgcg caccagatcg cccaggtcgg caagctgcga
     7261 tttctcgccg ccttcgccgc gcgcgaaatc gagatattcg tcgagcatgc gctccatctc
     7321 ggcgatgtcc tcgcgcagcg cctcggcctc cgggccggaa ggcatcatgg cgagctgcaa
     7381 gcggatgcgc gtgagcggcg tcttcagatc gtgactgacc ccggccagca tttcggtgcg
     7441 ctgctggacg tggcgctcga tgcgttcgcg catggtgatg aaggcttggg cggcgcggcg
     7501 cacctccgtg gcgccgtaag gcttgaagtc cggcacggcg cggcccttgc cgtaggcgtc
     7561 ggcggcgcgc gccagccgct cgatcgggcg cacctggttg cgcaggaaca agattgccac
     7621 cgtcagcagg atcatcgcgg aacccaccat ccagacgatg aagaggtccg gggccgagac
     7681 ggtgatgcgg tcgcgcgcca caatcgcgtg cagcacgccg tcatgcactt cgacgctgat
     7741 atccatcttg tgcttgcccg gcagggacgc cgtctcgaaa tggcgcgcct cgccgatctg
     7801 ctgggcgatg acttcgtcga gggcgatgtc gatggtgctg cggtagcggc ccgaagacgg
     7861 agtgatatgg gcgccgcgct ccaggctcag atcgtagcgc agttgcttgg cggcgagggc
     7921 gcggagttct tcgcgcagcg gcgagggata ggtgtcttct tgcgcgacga ggagggcgat
     7981 gtcggcggcc acgtcgcgcg ccatgcgccg cgtcgtgatg tcgaggtcgc gctcgaagaa
     8041 gacgtaggac acgatcgcct gcaacaggag catcggcgcg acgatgatga tgagcgaacg
     8101 gcttaacagg ccgcgcggca tcacgcgctt gagcaggcgg ccggggcgca gccgcgacaa
     8161 caccgctttg aggttctcaa accgcgcggt cggggacaag gacgtagcct acgccgcgga
     8221 cggtctgcag atagagcgga agcttggggt cctcctcgat tttgcgcctg agccgcgtca
     8281 cctgtacgtc gatcgagcgt tccagcgcca cgcccagccg ggcgcagaga tcggcgcgcg
     8341 agaaggaacg cccggcattg gcggcgaaga attgcagcag cgtgctttcc gagctggtca
     8401 ggtgcacggg tttgcccttg cgccggagct gaccgcgctc cggatcgtaa acgcagtcgc
     8461 ccatgcgcac ttcggcgagg gccttctcgg ccggcggggc ggcgcggcgc aacagcgcgc
     8521 tcacccgcag cagcaattcg cgcggctcga acggcttggc gagataatcg tcggcgccgt
     8581 gctcgaggcc ccggatgcgg tcctcggcct cgcttttggc ggtcagcatc aggatcggca
     8641 cggacgagcc ggcgcgcacc gatttggtca ggtcgaagcc ggattcgccg ggcatcatca
     8701 cgtcgaggat gagcaggtcg aaggccatgc ttttcatcag ggcgcgggcg tcggccgcgc
     8761 tgcccgccgc gctgacgcga aagccgttgg aggacaggta gcgctgcaac agcgtgcgca
     8821 gccgctcgtc gtcgtcgaca accagaaggt gaggatcctg cagcaccgtg ctcattcttt
     8881 ttccttccgg ccgccgccgc cgagacccag cagcactttg cgaaagccgt tcaccgcgtc
     8941 ctggcccgcc gccgtaaatg cggaaaccag caacgggcgc tgctcttccc agatggcgtc
     9001 ggccagcacg gcgcccgccg cggtcaaccg caatcgcctt gtacgccgat cctgcatgcc
     9061 gggcttgcgc tcgaccaagc cgtttttcaa caggtcattg agcacgcggt tcaggctttg
     9121 tttggtcact ttcaggaagc caagcaattc ggcgacggaa agccccgggt tgcgcgcaat
     9181 gaaatgtagg gcacgggcat gcgcgcgtcc caatcttgct ttggcgaggg ggtttgcgcc
     9241 ttgcgccgaa atatcgcgcg cggcaacgaa taacaaatcc aagccttgcc taagcgcctc
     9301 atcgctaagg tataatgttt tggtatcggg caacgtcccg ccgaatttta cgtcagacat
     9361 gctgacacaa ataggccgcc tgctcgggca aggcaagggg ctggaggact gcacaatggc
     9421 cgaggtgatc ccctttgacg accgcgatgg ttcgctctgg tacgacggca agctggtgcc
     9481 ttggcgcgag gccaagaccc atgtgctgac ccacgggctg cactatgcct cctgcgtgtt
     9541 cgaaggcgag cggctttatt ccgggcgcat ctacaagctc aaggaacaca ctgagcgtct
     9601 gttcgagagc gcgcgcctcc tcggcatgac cattccgtac acggaagacg acgtcaacgc
     9661 cgcctgcatg gcggccgtcc acgcgcaggg ctacaccgac ggctatgtcc gcccggtagt
     9721 gttccgcggc agcgagatga tggcggtctc cgctcagaag acgaaggtgc atgtcgccgt
     9781 cgcggcatgg ccgtggccgt cctatttcga tcccaaggaa aagctgaaag gcatccggct
     9841 cgacatttcc gattggcgcc ggcctgcgcc cgacaccgcg ccgaccaagg ccaaggccgc
     9901 cggtctctac atgatctgca ccatgtccaa gcacgtcgcc gaggccaagg gctatgccga
     9961 cgcgctgatg ttcgattacc gcggcttggt ggcggaagcg accggggcca atatcgtttt
    10021 cgtcaaggac ggcacgctgc acacgccgac gccggattgc ttcctcgacg gcatcactcg
    10081 ccgctcggtg ctggctttgg cgcggaagcg gcagattccc gtggtcgaac ggcacatcga
    10141 cgcgaaggaa ctggcggact tcagcgaagc cttccttgcc ggcacggcag ccgaggtgac
    10201 cccggtttcc gagatcgggc cgtatcattt caagccgggc gagatcagcg agatgctgat
    10261 gcacgactat gccgaagacg tccgccggcc ggtgtcggtg gtggatttga gcgcagcggg
    10321 gtgaattccc cctgctgtgt ggcggcgcgc ggccgcgccg gatcggcgaa ggcgtccgcg
    10381 taaccgggcc cgcgccttta acgagcttca ggccgacgtc tcgttttcgg ggtggggacg
    10441 ggcatgcgcc aagccggcgc gcagatagac atagcccgta cccaccgtca gcgccgcggc
    10501 cacccacaac gcgatatagg cgaaggcgag ggcgccgggc accaccttgt cggccaaggg
    10561 accgaggatc atcgcgccga tggcgatcat ctggatcgtg gtcttgattt tggcgaagaa
    10621 gctgacggga acgctgacat ccgctccggc caggaattcg cgcaggcccg agaccaggaa
    10681 ttcccgcgcc aggatcacca gc
//
LOCUS       DASWPU010000276         4757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_45444,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000276 DASWPU010000000
VERSION     DASWPU010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45444"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VGC27_05895"
     CDS             <1..1373
                     /locus_tag="VGC27_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0282136.1"
                     /translation="FDLTVINHIEPMDYDIYGRYDYYFGYSSPAFKSLLAQLRAAPDR
                     AKRIALVQSIQRRIADDAVNGFLFELPRLVVSDAKLAGFQGSAAIPSNDVSGAYFSEG
                     PSAVAGAPPANPATLSPLWRFAPVLLLLLLAYAGWRVGPAYLLSRVGYLLITLLVASS
                     AVFFVIALLPGDPAAFMLGLNATPEALAALHGQLGLDAPLWIRYVRWLTGLARGDFGV
                     SYTYHVPVARLIAERLLVSLPLAVYALAISLVFAIPLGLFTAARHGRISGEAASAVTQ
                     VGIAVPDFWLAILFVLLFSMTLRWFSGGGFPGWEFGVWPAIWGLTLPALALAIPQAAI
                     LARILSRSLLDVLDADYIRTARAKGLSRIQTLWRHAFPNAVIPVLTVLGLQFSFLIAG
                     AIIIENVFALPGLGRLLSQAVERRDLIVVQSVVVLLVLIVVLVSFIVDVAYALADPRL
                     RGGRNR"
     gene            1370..2206
                     /locus_tag="VGC27_05900"
     CDS             1370..2206
                     /locus_tag="VGC27_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0282137.1"
                     /translation="MIVRPEAWRRARRYLVPGALLVGALAGAGLLSFVWLPYDVLNLD
                     VADKLRPPSFDHPFGTDQLGRDVVSLIMIGARNSLGVALAAVVLGLGLGVPLGLLAAS
                     GRGWIDHAVMRLSDVVFAFPAVLTAILIMAVSGPSAWNVVIAVGIFNIPVFAKVARGA
                     ALVQWELPYIFAARVAGKGAAAISLEHVLPNVAGIITAQAAVQFSMGVIAEAGLSYIG
                     LGVQPPDQSWGRMLNEAQTLIALAPHLVVFPGLAILLAVLGFNLLGEGLHAAFDPRLA
                     RR"
     gene            2210..3823
                     /locus_tag="VGC27_05905"
     CDS             2210..3823
                     /locus_tag="VGC27_05905"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY0282138.1"
                     /translation="MTLLRVDKLSVTFGKKQVLNGTAFSLSAGEILAIAGESGSGKST
                     AALAIAHLLPAAARVSGSVLLEGDDLNAKGERALCGIRGRDIGLVFQEPMTALNPVMQ
                     VGDQIAESLRLHRRISRSEAREAVRNLLERVGLIDIPATRYPHELSGGQRQRVAIAIA
                     IACSPKLIIADEPTTALDVTTQAQVLALFRQLVREERIGLILITHDLAVVAETADRIA
                     IMNKGRIVEEGTTLQVLREPRHASTRELVEALSRFPDRISKPRNGRPLLEVSNLVRHY
                     GGRFLNRGTQTAVADVSFTVEQGESVGIVGESGSGKSTLLRAVLGLDRPNGGDVRIAG
                     TAISSARGKKRLDVRRKVQAVFQDPLSSFDPRHHIGRVVAEPLHLLGENLGRVERRKR
                     AEAALERVGLPARMADRYPHEFSGGQRQRIAIARALIIEPALIALDEAVSALDVSARA
                     QIVELLNGLSRDLGVAYLFVTHDLSLVRSITDRLIVMRAGRIVETGATGDVLSAPQHP
                     YTAALLAATPDLRRALGAGRTAAAADIAK"
     gene            3874..4335
                     /locus_tag="VGC27_05910"
     CDS             3874..4335
                     /locus_tag="VGC27_05910"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY0282139.1"
                     /translation="MSVAEVRAFFAEKAPDIVVIELEEVTATVDLAAQGHGVEPAQIA
                     KTLSLKAGGRNFLVVMRGDARLDNKKAKVAFGGKVSMLAPEEVEAVTGHPVGGVCPFG
                     LRTPLPVYCDVSLQAFDEVVPAAGSIRSAVRINPIRMAGLVGATWFDVAQV"
     gene            4545..>4757
                     /locus_tag="VGC27_05915"
     CDS             4545..>4757
                     /locus_tag="VGC27_05915"
                     /inference="COORDINATES: protein motif:HMM:NF018165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282140.1"
                     /translation="MASRRDLDPIWRALRQEAEELASQEHALAGLVRTTVLNQKRLED
                     ALSYHLARKIGGAVLSTILVREIVDQA"
BASE COUNT          718 a         1517 c         1537 g          985 t
ORIGIN      
        1 acttcgacct gacggtcatc aaccatatcg agcccatgga ctacgacatc tatggccgct
       61 acgattacta tttcggctat tccagtcccg cgttcaaatc gctgttggcc caattgaggg
      121 ccgcgccgga tcgcgccaag cgcatcgcgc tggtccaatc catccagcgc cggatcgcgg
      181 acgatgccgt gaacggcttt ctcttcgaac tgccgcggct tgtcgtcagc gatgccaagc
      241 tcgcggggtt tcagggcagt gcggccatcc cgtcgaacga cgtttcaggg gcgtacttca
      301 gcgaaggccc atcggcggtg gccggggcgc cgcccgcaaa ccctgcgacg ctatcgccgc
      361 tctggcgctt cgcccccgtg cttcttcttc tccttctcgc ttacgcgggc tggcgcgtgg
      421 gcccggctta tctgctgagc cgtgtcggct atctgctgat cacgctcctt gtcgcgagca
      481 gtgccgtgtt cttcgtgatc gcgcttcttc cgggcgatcc ggccgccttt atgttgggcc
      541 tgaacgcgac gcctgaggca ttggccgcgc tccacgggca gctcgggctc gatgcccccc
      601 tgtggatccg ctacgtccgc tggctcacgg gactggcgcg cggcgatttc ggcgtgtcct
      661 acacctatca cgtgcccgtc gcgcggctga ttgcggagcg gctcttggtg tcgttgccgt
      721 tggccgttta cgccttggcc atttcgctcg tcttcgcgat ccctttgggg cttttcaccg
      781 cggcccggca cggccgcatc agtggggaag ccgcttcggc cgtcacgcag gtcggcattg
      841 cggtgccgga cttctggctc gcgattctct tcgtccttct gttttctatg acgttgcgct
      901 ggttctccgg aggcggcttt ccgggttggg agtttggagt gtggcccgcg atttgggggt
      961 tgacgcttcc ggccctggcg cttgccatcc cgcaggccgc cattctggcg cgcattctga
     1021 gccgctcgct gcttgacgtg ctcgacgccg attacatccg caccgcgcgc gcgaaagggc
     1081 tctcccgcat ccagacgttg tggcgccatg cctttcccaa cgccgtcata ccggtgctca
     1141 ccgttttggg cctgcagttc tcattcctga tcgccggcgc gatcatcatc gagaatgtgt
     1201 tcgcgctgcc ggggctgggg cgtctgcttt cgcaggctgt cgagcgccgc gatttgatcg
     1261 tggttcagag cgtggtcgtg ctgcttgtcc tcatcgtggt gttggtgtcg ttcatcgtgg
     1321 atgtcgccta cgctttggcc gatccaaggc ttcggggagg gcgcaaccgg tgatcgtgcg
     1381 tcccgaggct tggcgccgcg cgcgccgtta tcttgtgccg ggtgcgcttt tggtcggtgc
     1441 actggctggt gccggcctgc tgtcgttcgt ttggcttcct tacgatgtgc tgaacctgga
     1501 cgtggcggat aaacttcggc cgccctcttt cgatcatcca ttcggaaccg accagcttgg
     1561 ccgcgacgtc gtttcgctca tcatgatcgg cgcgcgcaat tctctcggtg tcgcgctggc
     1621 ggccgtcgtc ttgggtctcg gattgggcgt tccgctcggg cttctggccg cctcgggccg
     1681 cggctggatc gatcacgccg tcatgcgctt gagcgacgtc gtgtttgcgt ttccggccgt
     1741 gctgacggcc attctgatca tggcggtgtc gggaccgagc gcatggaacg tcgtcattgc
     1801 cgtggggatc ttcaacattc cggtgttcgc gaaggtggcg cgcggcgccg ccttggtgca
     1861 atgggagttg ccgtacatct tcgcggcccg cgtcgcgggc aagggtgccg cggcgatttc
     1921 gctcgagcac gtcctgccca acgtggcggg catcattacg gcccaggcgg ccgtccagtt
     1981 ttccatgggc gtcatcgcgg aagcgggctt gtcctacatc ggtctcggcg tgcagccgcc
     2041 cgatcaaagt tgggggcgca tgctgaacga ggcgcagacc ctcatcgccc tcgcgccgca
     2101 tctggtcgtc ttccccggcc tcgccatcct gctggccgtt ctcggattca atctgctcgg
     2161 cgaaggcctg cacgcggcct tcgatccgcg ccttgcgagg cgttgagcga tgacccttct
     2221 gcgtgtcgat aagctttcgg tgaccttcgg gaaaaagcag gtcttgaacg ggaccgcatt
     2281 ctcgctgtcc gcaggcgaga ttctggcgat cgcgggcgaa tccggctccg gcaagtccac
     2341 cgccgccctg gccattgcgc atctcttgcc ggctgcggcg cgtgtttccg gctccgttct
     2401 gctcgagggc gacgacctga acgccaaagg cgaacgcgcg ctttgcggca tccgcggccg
     2461 tgacatcgga ctggtgtttc aggaacccat gacggcgctg aacccggtca tgcaggtcgg
     2521 agatcagatc gcggagagct tgcgtctcca tcgaaggatt tcccgaagcg aagcccggga
     2581 ggcagttcga aatcttctcg aacgcgtcgg gctgatcgat atacccgcaa cgcgctatcc
     2641 gcacgagctg tcgggcggcc agcgccagcg cgtcgccatc gccatcgcga ttgcctgttc
     2701 gccgaagctc atcattgccg acgagccgac caccgccctc gacgtcacca cgcaggcgca
     2761 ggtccttgcg ctgttccgcc aactcgtgcg cgaagaacgc atcggcctca tcctgatcac
     2821 gcatgatctc gccgtcgtcg cggaaacggc cgatcgcatt gcgatcatga acaagggccg
     2881 gatcgtcgaa gagggcacga cgctgcaagt gttgcgcgaa ccccggcatg cctcgaccag
     2941 ggaactggtc gaggccctgt cgcgcttccc cgaccggata tccaagcctc ggaacggccg
     3001 gccgcttctg gaagtttcca atctggtgcg ccattacggc gggcggttcc tgaaccgcgg
     3061 gacgcaaacc gctgttgcag acgtcagttt caccgtcgaa caaggcgaga gcgtggggat
     3121 cgtcggggaa tccggcagcg gcaagtcgac cctgcttcgc gcggtgctgg gtctcgaccg
     3181 gccgaacggc ggcgatgttc gcatcgcggg cacggccatt tcctccgcac gcggaaagaa
     3241 gcgcctggac gtgcgccgga aggtccaggc cgtgttccag gacccgctca gcagtttcga
     3301 tccgcgccat catatcggcc gggtggtggc cgagcctctg catctcctgg gcgaaaacct
     3361 tggccgggtg gagcggcgca agagggcgga ggccgcgctc gaacgcgtcg ggcttccggc
     3421 gcgcatggcg gaccgctatc cgcacgagtt ttccggcgga cagcggcagc gcatcgccat
     3481 cgcgcgggcc ttgatcattg aacccgccct gatcgcgctg gacgaagccg tctcggcgct
     3541 ggacgtctcg gcgcgcgcgc agatcgtgga gctgttgaac ggcttgtcgc gcgacctcgg
     3601 cgtcgcctat ctgttcgtga cgcacgatct gtcgctggtc cgttccatta cggatcggct
     3661 gatcgtgatg cgtgccggcc ggatcgtcga aacgggcgca acgggcgacg tgctttccgc
     3721 gccgcaacac ccttatacgg cggctctgct ggccgccacg cccgatctcc ggcgggcact
     3781 tggggccggc cgcacggcag cggctgccga tatcgcgaaa tgaggttaag gtttgaaacg
     3841 tacgatccct tctgaaaacg acgtgaattg acgatgagtg tcgcagaggt tcgcgctttc
     3901 tttgccgaaa aggcgcctga tatcgtcgta atcgaactgg aagaggtcac cgcgacggtc
     3961 gatcttgccg cgcaagggca tggcgtggaa ccggcgcaga tcgccaagac cttgtcgctc
     4021 aaggccggcg ggcgaaactt cctcgtcgtc atgcgcggcg acgcgcgtct cgacaacaag
     4081 aaggccaagg tcgccttcgg cggcaaggtg agcatgctcg ctcccgagga ggtcgaggcc
     4141 gtgacggggc atccggtcgg cggtgtctgc ccgttcgggc tgcgtacgcc gctgcccgtc
     4201 tattgcgatg tatcgctgca agcctttgac gaagtggtcc cggccgcggg ctcgatccgt
     4261 agcgccgtgc ggatcaatcc cataaggatg gccgggctcg tcggcgcgac gtggttcgac
     4321 gtcgcgcaag tgtagaccgc acatccgcat ttttaggaaa gaaaattgcg gactggctgc
     4381 ccaaaggcgc cagcccgcca agttcaggga ggaaacaccc agaagggatc ttccgaggaa
     4441 actggagttt aacactacta aatcgatagt caagatggta tatatagcgt cgtcacgtta
     4501 ggttgggttc cgaaacccgc gtgaaatttg gaggagcgcc ggccttggca tcccgccggg
     4561 atctcgaccc tatctggcgc gctttgcgcc aggaagccga agaactggcc tcgcaggagc
     4621 atgccttggc cggtctcgtc cgtaccacgg ttctcaacca aaagcggctg gaagatgcgc
     4681 tcagctatca tctcgcgcga aagatcggcg gcgcagtgct gagcacgatt ctcgtgcgcg
     4741 agatcgtcga ccaggcc
//
LOCUS       DASWPU010000277         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_112772,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000277 DASWPU010000000
VERSION     DASWPU010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112772"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VGC27_05920"
     CDS             <1..276
                     /locus_tag="VGC27_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0282141.1"
                     /translation="PKGAGSVFTFGVKGGFEAGKKLVDSVKLFSHLANIGDTRSLIIH
                     PASTTHSQLSAEELDKAGAGPDVVRLSVGIESVDDLIADLNQGLASA"
     gene            407..1387
                     /gene="cysK"
                     /locus_tag="VGC27_05925"
     CDS             407..1387
                     /gene="cysK"
                     /locus_tag="VGC27_05925"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY0282142.1"
                     /translation="MFGRKKASDHKPGRHRIYNSITETIGDTPLVRLKRLPQQAGVKA
                     DILLKLEFFNPIASVKDRIGVSMIEALEREGKISPGKNTLVEPTSGNTGIALAFVAAA
                     KGYKLILTMPESFSIERRKMLALLGAQLVLTEAAKGMRGAIEKAQEIVAATPDAVSPR
                     QFDNPANPDIHRRTTAEEIWNDTNGKVDVVVAGSGTGGTLTGVGQVLKKYKKSKLRII
                     AVEPEDSPVLSGGKPGPHKIQGIGPGFIPSILDRSVIDEIITVSNETALDTARKVARQ
                     EGIPVGISAGAAIAVAFDVGARPEYAGKTIVTIVPDFAERYLSTALFEGV"
     gene            1655..>2787
                     /locus_tag="VGC27_05930"
     CDS             1655..>2787
                     /locus_tag="VGC27_05930"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF004650.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEY0282143.1"
                     /translation="MADYGFDTLSVHAGAQPDPATGARATPIYQTTAFVFEDADHAAA
                     LFNLQVFGNIYTRIMNPTNAVLEERIAALEGGRAGLSVASGHAAQLVALHTLMGPGDE
                     VVAARQLYGGSINQFGHAFAKFDWHAKWADGTKPESFQAALSPKTKAVFIESIANPGG
                     IITDIEAIAKIAHGAGVPLIVDNTLASPYLIRPFEWGADIVLHSATKFLGGHGNSMAG
                     LIVDGGKFDWGSGKFPTLSEPCPSYHGMKFWETFGDMAYAIACRVLALRDLGPALAPM
                     NAFLVITGIETLPLRMQKHCDNALAVARWLKQHPKVAWVNYAGLEDNPCFALHRKYCP
                     KGAGSVFTFGVKGGYEAGMKLVNSVKLFSHLANIGDTRSLIIHP"
BASE COUNT          560 a          874 c          861 g          492 t
ORIGIN      
        1 cccaaaggcg cgggcagcgt gttcaccttc ggcgtcaagg gcggcttcga agccggcaag
       61 aagctggtgg attcggtgaa gctgtttagc catctcgcca acatcggcga cacgcgcagc
      121 ctcatcattc acccggcatc gaccacccac agccagcttt ccgccgagga actcgacaaa
      181 gccggcgccg ggccggatgt ggtgcggctt tccgtcggca tcgaaagcgt tgacgacctt
      241 attgcggatt tgaatcaggg gctggcgtcg gcttagtaca attcgaacat gcgccgcacc
      301 gccagcctcg aagtgcatgc actcgttttt gccggcgccg gcgttgccgg cgatccgcca
      361 tcggacgcgc gaacagcgcc cgatttcaca atttaggaga ttgaccatgt ttgggagaaa
      421 aaaggcgtcc gaccacaagc ccggccgcca ccgtatctac aattcgatca ccgagaccat
      481 cggcgacacg cctctcgtgc gcctgaagcg cctgccgcag caggccgggg tcaaggccga
      541 catcctgctc aaactcgaat tctttaatcc tatcgccagc gtcaaggacc gcatcggcgt
      601 gtcgatgatc gaagcgctgg agcgcgaggg caagatttct ccgggcaaga acactctggt
      661 ggagccgacc agcggcaata ccggcatcgc gctcgccttc gtggcggcgg ccaaaggcta
      721 caagctgatt ttgacgatgc ctgaatcctt ttccattgaa cggcgcaaga tgttggcgtt
      781 gctgggggcg caactggtgt tgacagaggc ggccaaaggc atgagaggcg ccatcgaaaa
      841 ggcgcaggag atcgtcgccg caactcccga tgcggtcagc ccgcgacaat tcgacaatcc
      901 ggccaacccc gacattcatc ggcgcaccac ggctgaggaa atctggaacg acacaaacgg
      961 caaggttgac gtagtggtcg ccggttccgg caccggaggc acgctgaccg gcgtcgggca
     1021 ggtcctcaag aagtacaaga agtcaaagct gcggatcatt gccgtggagc cggaggattc
     1081 cccggttctg tcgggcggca agccgggccc gcataagata cagggcatcg gaccgggctt
     1141 cataccctcg atcctcgacc gcagcgtgat cgacgagatc atcaccgtct ccaacgagac
     1201 cgcgctcgac accgcgcgca aagtggcgcg gcaggaaggc attcccgttg gcatctccgc
     1261 gggagcggcg attgcggtag ccttcgatgt cggcgcgcgc ccggagtacg ccggcaagac
     1321 gatcgtgacg atcgttccag acttcgccga gcgctatctt tccacggcgc tgttcgaagg
     1381 cgtttgatcg cgcgggcgcg caatatcccc attctcccct caaagggccg catcctggtg
     1441 cggccctttc tgtatgtcag gaatgcgttg cgacgcggga cgccgcatcg gcctgaagcc
     1501 ggaagatcgc gggccgtatt ccgggttgga atagtgtgtc cgtcttgaac cggcttgaat
     1561 tgaattcaaa aataagagaa gtgggagggc ccctcgcaaa agccgaaaac gaatagaggc
     1621 ggccggagaa agcccgaatc acaaggagtt acagatggct gactatggtt tcgacacctt
     1681 gagcgtccac gccggcgccc agcccgatcc ggccaccggc gcgcgcgcca cgccgatcta
     1741 tcagacgacg gccttcgttt tcgaggatgc ggaccacgcc gcggcgctgt tcaatcttca
     1801 ggtcttcggc aacatctaca cgcggatcat gaaccccacc aacgcggtat tggaggagcg
     1861 catcgcggcg ctggaaggcg gacgcgccgg gctgtcggtg gcatccggtc acgccgcgca
     1921 attggtcgcg ctgcacacgc tgatgggtcc gggcgacgaa gtcgtggccg cgcgccagct
     1981 ttacggcggc tcgatcaatc agttcggcca tgccttcgcc aagttcgatt ggcatgcgaa
     2041 atgggccgac ggcaccaaac cggagagctt ccaggccgct ttgtcgccga aaacgaaagc
     2101 cgtgttcatc gagagcatcg ccaatccggg cggcatcatc accgacatcg aggcaatcgc
     2161 gaagatcgcg cacggggccg gcgtgccgct gatcgtcgat aataccctgg cgtcgcctta
     2221 cctcatccgt ccgttcgaat ggggcgccga catcgttctg cattcggcga cgaagttcct
     2281 cggcggccac ggcaattcga tggccggcct gatcgtggac ggcggcaagt tcgattgggg
     2341 cagcggcaag ttcccgacct tgtcggagcc ctgcccgtcc tatcacggta tgaaattctg
     2401 ggagacgttc ggcgacatgg cctatgccat cgcctgccgc gtgctggcgc tgcgcgatct
     2461 cggcccggcg ctggcgccga tgaacgcctt cctggtgatc accggcatcg agaccctgcc
     2521 gctgcgcatg cagaagcatt gtgacaacgc gctggccgtg gcccgctggc tgaaacagca
     2581 tcccaaagtc gcctgggtga attacgccgg cctggaagac aacccctgct ttgccctgca
     2641 ccgcaaatac tgccccaagg gcgcgggcag cgtcttcacc ttcggcgtca agggcggcta
     2701 cgaagccggt atgaagctgg tcaattcggt gaagctgttc agccatctgg cgaacatcgg
     2761 cgacacgcgc agcctgatca tccatcc
//
LOCUS       DASWPU010000278         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_112778,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000278 DASWPU010000000
VERSION     DASWPU010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112778"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2554)
                     /locus_tag="VGC27_05935"
     CDS             complement(<1..>2554)
                     /locus_tag="VGC27_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEY0282144.1"
                     /translation="PTHLKLLLHVLAEGVPPRLRTLIVGGEDLKTGLARAIHTSLGGR
                     VDIYNEYGPTEATVGCMIHKYSPDQDTALSVPIGTAIDHSSVFVLDRHGNPVPLGVSG
                     ELHIAGEGLAAGYLNNPALTDERFVVKRLGGVPLRLYRTGDLARILPDGKLEYLGRND
                     DQVKIRGFRVELGEVEAHVARVPGVAAAAVTHRVDAAGDSHLCAYVELSGAVSPAEVR
                     ARLAEQLPAHMVPTWITPLAQLPVGSGGKIDKARLPNPQASGADTHSYAAPRSPHEAA
                     MATIWEHILGVEKIGLDDNFFESGGQSLKATIMTVLMNRQLGVSFALQDVFAHPTIRQ
                     LAALMQPDRPESRAIAPVGERPYHAASSSQKRLFILNRMAPLAVQYNVPWAVRISGVF
                     DAARWQEAFAALIARHEPLRTSFSLIDDDVVQRVHNEVDFAFEAITFAECLDLNREIA
                     RFAKPFDLGKAPLLRAEIIKTGDGVHTLIVDTHHIVTDGLSFRVILDELVSLYRGQAL
                     EPLEIQYKDYAAWQRARSQSDDVKAQRAYWTKLFEGELPVLNLPTDFPRSAVQSFAGG
                     LLRFETGREVAEGLHAIARRCGVTMHMVLLAAYAILLSKHTGQDDLVVGTPVSGRNHP
                     SLQKLVGMFVHTLALRMRPEKGLSFEAFLERVRDSLLEAYDNQDCQFDELVEHLAVER
                     DLSRNPLFDTVFATLDTPARRMEFDGTAIELLDFDWNISKFDLTLLASERDGTLAFEL
                     EYCAGLFRRDTIERMAAHYGRLLEQIAADPARSIAAYGTLTEAERRQTLVAFNRTEHA
                     YPTQQSIHAIIEDQVERTPANLALVHGGTALTYCEMNRRANQLARLLLRNGAA"
BASE COUNT          441 a          777 c          850 g          486 t
ORIGIN      
        1 tagccgcgcc gttgcgcaag agcagccggg cgagctggtt cgcccgccgg ttcatctcgc
       61 aataggtgag cgcggtgccg ccgtgcacca gcgccaggtt tgcgggcgtt ctttccacct
      121 gatcttcgat gatggcgtgg atgctttgtt gcgtgggata cgcgtgttca gtgcggttga
      181 atgcaaccag ggtctggcgt ctctcggcct ccgtcagggt accgtaggcc gctatgctgc
      241 gggccggatc ggcggcgatc tgctcgagaa ggcggccgta atgcgccgcc atccgctcga
      301 ttgtgtcacg ccggaagagc cctgcgcagt attcgagttc gaaggccagc gtgccgtcgc
      361 gctcgctcgc gagcaaggtg agatcgaatt ttgagatgtt ccagtcgaag tcgagcagtt
      421 cgatggcggt cccgtcgaac tccatccgcc gcgccggggt gtcgagcgtg gcgaataccg
      481 tatcgaacag cggatttcgg ctgaggtcgc gttccaccgc gagatgctcc acaagctcgt
      541 cgaactggca gtcctgattg tcgtaggctt cgagcagcga atctctcacc cgttcgagga
      601 acgcctcgaa actgagcccc ttctccggcc tcatccgcag cgccagcgta tggacgaaca
      661 tgcccaccag cttctgcagc gacgggtggt tgcgccccga aaccggcgtt ccgacgacaa
      721 ggtcgtcttg gcccgtatgc ttcgaaagca aaatggcata ggcggccagc aacaccatgt
      781 gcatggtgac gccgcaacgg cgggcaatcg cgtgcagtcc ttcggcaacc tcgcggccgg
      841 tctcgaagcg caagagcccg ccggcaaagg attgcacggc acttctcgga aagtcggttg
      901 gcaggttgag aaccggcagt tcgccttcga acagcttcgt ccagtaggcc ctttgggcct
      961 tgacgtcgtc actttgggag cgggcacgct gccacgccgc gtagtccttg tattgaatct
     1021 ccaacggctc aagcgcctgc ccacggtaga gcgagacgag ttcgtcgagg atgacgcgga
     1081 acgacagtcc gtcggtcacg atatggtgcg tgtcgacaat gagtgtgtga acgccgtcac
     1141 cggtcttgat gatttccgcc cgcagcagcg gcgccttgcc gaggtcgaag ggcttggcga
     1201 agcgcgcgat ttcgcggttc aggtccaggc attccgcaaa ggtgatagcc tcgaacgcga
     1261 agtcgacttc attgtgcacg cgctgcacga cgtcgtcgtc gatgagcgag aaagatgtgc
     1321 gcagcggctc gtgcctggcg atcagtgccg caaaggcctc ctgccagcga gccgcgtcga
     1381 acacgccgct gatccggacg gcccagggaa cattgtactg gacggcaagc ggcgccatcc
     1441 ggttcaggat gaagagccgc ttctgcgacg acgacgcggc gtggtacggc cgttccccga
     1501 ccggcgcgat agcgcggctt tccggccgat cgggctgcat caaggcggcg agttgccgga
     1561 ttgtggggtg ggcgaacacg tcctgcaggg cgaagctgac gccaagctgg cggttcataa
     1621 gcaccgtcat gatggtcgcc ttgagggatt ggccgccgga ctcgaagaag ttgtcgtcga
     1681 gtccgatctt ttcgactccc aggatatgtt cccatatcgt cgccatcgcg gcttcgtgcg
     1741 gcgagcgggg cgcggcgtag gaatgcgtgt ctgcgcccga cgcttgggga ttgggaagcc
     1801 tcgccttgtc gatctttccg ccgctcccca ctggaagttg cgccagagga gtgatccagg
     1861 ttggaaccat gtgggccgga agctgctctg caagcctggc gcgcacctcg gcggggctga
     1921 cggcgccgct tagttcgaca taggcacaga ggtgggagtc gccggccgca tcgactctgt
     1981 gcgtcacggc ggcggcggcg acccccggaa cacgcgcgac atgggcttcg acctcgccaa
     2041 gctccacccg gaaaccgcga atcttcacct gatcgtcgtt ccgccccaga tactcaagct
     2101 tgccgtccgg caagatgcgg gcgaggtcgc ccgtcctata gaggcgcagc ggcacgccgc
     2161 cgagccgctt cacgacgaag cgttcgtctg tgagcgcagg gttgttgagg taccccgcgg
     2221 cgagtccctc tccggcgatg tgaagttccc cgctgacgcc caacggaaca gggtttccat
     2281 gcctatccag gacgaagacg ctcgaatggt cgattgccgt tccgatgggt acggagagcg
     2341 cggtgtcctg gtccggacta tatttgtgga tcatgcagcc gaccgtcgcc tcggtcggtc
     2401 cgtattcgtt gtagatgtcg acgcgcccgc caaggctggt gtgaatggct cgggcgaggc
     2461 cggtcttgag gtcttcgccg ccgacgatga gggtccgcag ccgcggcggg actccttcgg
     2521 caagcacatg cagcagcagt ttcagatgcg tggg
//
LOCUS       DASWPU010000279         4318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_21068,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000279 DASWPU010000000
VERSION     DASWPU010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21068"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /gene="tdh"
                     /locus_tag="VGC27_05940"
     CDS             complement(<1..1003)
                     /gene="tdh"
                     /locus_tag="VGC27_05940"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY0282145.1"
                     /translation="MKALVKSKAVEGVWLEDVPEPSYGIDDVLIKVLKTGICGTDVHI
                     YNWDAWSQKTVKPGTVIGHEFVGRIVAVGSNVQSHKAGDLVSAEGHIVCGYCRNCLAG
                     RRHLCANTLGVGVNRNGAFAELVAVPAMNIWTCDAKIPQELYAIYDPLGNAAHTALSF
                     DLVGEDVLITGAGPIGVSAVPMAVKAGARYVVVTDVNDYRLALAQKMGATAAVNVKTQ
                     DLREVMKKLGMQEGFDVGLEMSGNPEGFRQMLDTMAHGGKIAMLGIQPEATGIDWNTV
                     VFKGLTIRGIYGRMFGETWYKLTSMIQSGMDIAPVITHRFSYKDFQKGFDVMRSGNSG
                     "
     gene            complement(1391..2743)
                     /gene="creD"
                     /locus_tag="VGC27_05945"
     CDS             complement(1391..2743)
                     /gene="creD"
                     /locus_tag="VGC27_05945"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEY0282146.1"
                     /translation="MATQDTSSSSSRVARSPGLKFLIIIVLTFVMAIPLFLIQLALSD
                     RQTTAAGAAGDIAAGWGGPQVVAGPVLLLPYTVLRDQVIDGKQVQSTQRLTALLLPET
                     LKSDVRATGEARWRGIFAVPVYRAAIALHATFDKSAMASLAPPDAHVLWNEAATAILV
                     SDAHGLADNVTLTLNGRAVPFEPGLGTYGSNGRISGIQAPLELSEPADLVLDTNFTLL
                     GSREFSVSPLGRRTVAKIDSPWPSPSFFGAFLPTERKVEKTGFSASWTVPYLARGFGQ
                     SFSDSRVGMDVLLKPAFGVRFYQPVEHYQLVQRSLKYSILFVALAFLVFFVVETVSPK
                     RMHFVQYAMVGAAQVLFYLLLLSLSEHLGFASAYLIASVATIALTSLYAVSALANKFR
                     AVVLCAILSALYGLLYIILNAEDYALLIGSSVLFAALAGTMYFTREIDWYRVTEEHVP
                     "
     gene            complement(2831..>4318)
                     /locus_tag="VGC27_05950"
     CDS             complement(2831..>4318)
                     /locus_tag="VGC27_05950"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEY0282147.1"
                     /translation="FNGDSVEAKNRSIFDTAAPSREWAEALHSFAWLPPLSAAGGDAA
                     RTLATNLIGQWLKRNARYSEPAWLPHVIARRLVHIFGHGRFVLTNSEMLWRSKVFVSL
                     REQSRMLARIAGEAPDGLPRLEAAAALALSGACLSDSPHRLEAGLARLEAEIARQILP
                     DGGHISRSPEVLVHAYRHLVMVMDALTATGHAVPHALRSAHDRMAPMIRFFRHGDGAL
                     ALFNGGGESDARMIAGLLARDEVRGQPFAHARHSGYHRLAAGRTLMLMDCGKPPPGAF
                     STGAHAGCLAFELSANAQRIVVNCGSASASGQRKWESALRATAAHSAVTLADCSTAVI
                     LSEGWVRNLLGPRLIGGVNEIETRRVETANGWTVEASHDFYLRLFGIRHERELTMSPQ
                     GLALTGADRLLPQKDRKGAAVPFAVRFHIHPDVRLSRSQSGDVILKLANGEGWRFRAG
                     VPIAVEESIYLGGDTVRRAEQLVLSGTVKDAQVEIAWIFEQIANN"
BASE COUNT          842 a         1376 c         1365 g          735 t
ORIGIN      
        1 ttcccgaatt gcccgaacgc atcacatcga agcccttctg gaaatcctta taggagaagc
       61 ggtgggtgat gaccggcgcg atgtccatgc ccgactggat catgctcgta agcttgtacc
      121 aggtctcgcc gaacatccgc ccgtagatgc cgcgtatcgt caggcccttg aacaccacgg
      181 tgttccaatc gataccggtc gcttcgggct ggatgccgag catggcgatc ttgccgccat
      241 gggccatggt gtcgagcatc tgccggaagc cttccgggtt gcccgacatt tccagaccga
      301 cgtcgaagcc ttcctgcatc cccagctttt tcatcacctc gcgaaggtcc tgggtcttca
      361 cgttgaccgc ggcggtggcg cccatcttct gcgccagcgc gagacggtaa tcgttcacgt
      421 cggtgaccac cacgtagcgc gcccccgcct tcaccgccat cggcaccgcc gagacgccga
      481 tggggccggc gccggtgatc agcacgtcct cgccgaccag atcgaacgac agggccgtgt
      541 gcgccgcgtt gcccagcgga tcgtagatgg cataaagctc ctgcgggatc ttggcgtcgc
      601 aagtccagat gttcatcgcg gggacggcga ccagttcggc aaaggcgccg ttgcggttga
      661 cgccgacgcc cagcgtgttg gcgcagagat ggcggcggcc ggccaggcag ttgcggcaat
      721 agccgcaaac gatatggcct tcggcgctga ccagatcgcc ggccttgtgg ctttgcacgt
      781 tcgagccgac cgcgacgatg cggccgacga attcatggcc gatcacggtt cccggcttga
      841 cggtcttctg ggaccaagcg tcccagttgt agatatgcac gtcggtgccg cagatgccgg
      901 tcttcagcac tttgatcagc acgtcgtcga tgccgtagga cggctccggc acgtcctcga
      961 gccaaacgcc ttccaccgcc ttggatttga cgagtgcttt catggaatcg cgcctttcgc
     1021 cgataaaggc ggcgggctta tcaaagcacc ctcgccgctg caatgccgtt gtaatttacc
     1081 gcacgatctt tgcgccaaac ggccgcctac atttgcggat cgtgcgcccg gagcgttcga
     1141 ccggcacagc cggaaagaca taaatcgttg aagcggctgg aaattgcctt tgaggttgtt
     1201 ttcgactttt cgatgggggt gccccccgga ctttttctgc atcacttctt tgaattcaat
     1261 tccccgagcc tcagtacgtc gacaatatcg aagtgatgtg ggaacatgct tcgccgcctt
     1321 caacccggtg cggcaaaagg gagcgcaacc cgacggggcc acgctcccct cgctcgcggc
     1381 ggatcggccg ctacggcacg tgctcttcgg tcaccctgta ccaatcgatc tcgcgggtga
     1441 aatacatggt tccggcaagc gccgcgaaca gcacgctgga gccgatcaga agcgcgtaat
     1501 cctcggcatt gaggatgatg taaagcaggc catacagagc cgacaggatg gcgcagagca
     1561 cgaccgcgcg gaacttgttc gccaacgcgc tgacggcata gagcgacgtc agggcgatgg
     1621 ttgcaaccga agcgatcaaa taggccgacg cgaagccaag gtgctcggag agggacagca
     1681 gcagcagata gaacaaaacc tgcgccgcgc ccaccatcgc atactggaca aagtgcatcc
     1741 gctttggcga aaccgtttcg acgacgaaga aaacaaggaa cgccagcgcc acgaacagaa
     1801 tcgagtattt cagcgagcgc tgaaccaatt gataatgctc taccggctgg tagaagcgaa
     1861 cgccgaacgc gggcttcagc agcacatcca ttccaactct actgtctgag aaactctgac
     1921 caaagccgcg ggcgagataa ggcacggtcc aagaggcgga gaaaccggtc ttttccacct
     1981 tacgctccgt cggcaggaac gcgccgaaga aactgggcga cggccaaggc gagtcgatct
     2041 tcgcgaccgt ccggcggcca agcggcgaaa cgctgaattc gcgcgagccc agcagcgtga
     2101 agtttgtgtc gagcacgaga tccgccggtt cgctcaactc cagcggagcc tggatgccgg
     2161 aaatcctgcc attgctgccg tacgtcccca gacccggttc aaacggcacc gcgcggccgt
     2221 ttaaggttag cgtgacattg tccgcgaggc catgggcgtc agagaccaag atcgccgtgg
     2281 cggcttcgtt ccacagcaca tgcgcatccg gcggggccag cgaagccatc gccgatttgt
     2341 cgaacgtggc gtggagcgcg atggcggcac gatagaccgg caccgcaaaa attccgcgcc
     2401 agcgcgcttc tccggtggcg cgaacgtcgg atttcagggt ttccggcaac agcaaggcgg
     2461 tcagccgttg cgttgattga acctgttttc cgtctatgac ctgatcgcgc aagaccgtgt
     2521 agggcagcag gagcaccggc cccgcgacga cctgcggtcc gccccaacct gcggcgatgt
     2581 cgcccgcggc accggccgcc gtcgtctggc ggtccgacag cgcgagctgg atcaggaaca
     2641 gcggaatcgc cattacgaac gtcaacacga tgatgatcag aaatttgaga ccgggcgagc
     2701 gcgctacgcg cgaagaagat gatgaggtgt cttgcgttgc catcgaaccc ctccgttggc
     2761 tgacactgaa tgcttagtgt ggaacatggc gcgatcgagg cgcgagaaaa gcaacttaag
     2821 ggcgacagaa ttaattgttg gcaatctgct cgaaaatcca ggcgatttcg acctgggcgt
     2881 ctttcacggt tcccgacagg acgagttgct cggcgcggcg cacggtgtcg ccgccgagat
     2941 agatgctctc ctctaccgcg atgggcacgc cggcgcggaa gcgccagcct tcgccgttgg
     3001 caagcttcag gatcacatcg ccgctctggg accgcgacaa gcgcacgtcg ggatggatgt
     3061 ggaagcgcac agcaaaggga acggcggcgc ctttgcggtc cttttggggc agcagccggt
     3121 cggcgccggt cagcgccagc ccttgcggcg acatggtcag ctcgcgttcg tggcggatgc
     3181 cgaacaggcg gagatagaaa tcgtggctcg cctccaccgt ccagccattg gcggtttcga
     3241 cccgccgcgt ctcgatctcg ttcacgccgc cgatcagccg gggccccaac aggttccgca
     3301 cccagccctc gctcaggatc accgccgtcg agcagtcggc cagagtgacg gctgaatgcg
     3361 cggccgtggc cctcaatgcg ctttcccatt tgcgttggcc cgaggcgctc gccgaaccgc
     3421 aattgacgac gatgcgctgg gcgttggcgc tcaactcgaa agcaaggcag ccggcatggg
     3481 cgccggtgga gaaggcgccg ggcggcggct tgccgcaatc catcagcatc agcgtgcgcc
     3541 ccgccgccag acggtgatag ccggaatggc gggcatgggc gaaaggttgg ccgcggactt
     3601 catcgcgcgc cagaagcccc gcgatcatcc gcgcgtcgct ctccccgccg ccgttgaaca
     3661 aggccagcgc cccgtcgccg tgacggaaga agcggatcat cggggccata cggtcgtggg
     3721 cgctgcgcag cgcatgcggc accgcgtggc cggtggccgt cagcgcatcc atcaccatca
     3781 ccagatgacg ataggcatga accagcacct cgggcgagcg gctgatatgg ccgccgtcgg
     3841 gcaggatttg ccgcgcgatt tcggcttcca ggcgcgccag cccggcttcg agccggtgcg
     3901 gactgtcgga caagcaggcg cccgacaaag ccagcgccgc cgccgcctcc agccgcggca
     3961 gaccgtcggg cgcctcgccg gcgatgcgcg ccagcatgcg cgactgctcg cgcagggaga
     4021 cgaacacttt cgaccgccac agcatctcgg aattggtcag gacgaaacgc ccgtgaccga
     4081 aaatgtggac cagacgccgc gcgatgacgt gcggcagcca ggccggttcg gaatagcgcg
     4141 cgttgcgctt cagccattgg ccgatgagat tggtcgccag cgtgcgcgcg gcgtcgccgc
     4201 ccgccgcgga caagggcggc agccaggcaa aactatgcag cgcctcggcc cattcgcgcg
     4261 acggcgcggc ggtgtcgaag atcgagcggt tcttcgcctc gacggagtcg ccgttgaa
//
LOCUS       DASWPU010000280         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_87814,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000280 DASWPU010000000
VERSION     DASWPU010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87814"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VGC27_05955"
     CDS             <1..459
                     /locus_tag="VGC27_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282148.1"
                     /translation="GMKLVAHAGEEGQAEYIWQALDLLHADRIDHGNNSLHDHKLVAR
                     LAAEQMTLTVCPLSNLKLCVVKSLKHHPLQRMLDLGLRATVNSDDPAYFGGTVNDNFV
                     AVAEALSLTKAEIVTLAKNSFLGSFLSETEKAHHLAEVAAYVAARGIAEN"
     gene            561..755
                     /locus_tag="VGC27_05960"
     CDS             561..755
                     /locus_tag="VGC27_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282149.1"
                     /translation="MSKPFTIISALLLLAIAAAHAYRAYYGIDVLVAGHAIPIWASWV
                     STAVAAVLGIMLFVEAPRRK"
     gene            complement(827..994)
                     /locus_tag="VGC27_05965"
     CDS             complement(827..994)
                     /locus_tag="VGC27_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282150.1"
                     /translation="MEKSHVTVHAEGAGLFWLAGWLFTVGYLKLAFWPGALAILIWPY
                     HIGVAVAALHP"
     gene            complement(1384..>1913)
                     /locus_tag="VGC27_05970"
     CDS             complement(1384..>1913)
                     /locus_tag="VGC27_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282151.1"
                     /translation="GLKNADGTRAYPKAAVWVAEEEAAYWLGSDLDTMPEKARAPFKT
                     AREVMDIYRAAGAFHRFKAGETIVPGLRSVPIPGHTPGQTAYLFESKGQALLAMGDIV
                     HSYAVQFPLPDVTISSDTDAAAAAATRRTLFASLAKDGTAIAGSHLPFPGIGHIRKDG
                     DGFLWVPVEYEVTAK"
BASE COUNT          353 a          587 c          600 g          373 t
ORIGIN      
        1 ggaatgaagc tcgtcgccca tgcgggcgag gaggggcagg cggagtatat ctggcaggcg
       61 ctcgatctat tgcatgccga ccgtatcgat cacggcaata attcgctcca cgatcacaag
      121 ctggtcgcgc ggctcgccgc cgagcagatg acgctgaccg tctgcccgct ctccaatctc
      181 aaactctgcg tggtgaagag tctgaagcac cacccgctcc agcggatgct ggacctgggc
      241 ttgcgcgcga cggtgaactc cgacgacccg gcctattttg gcggcactgt gaatgacaat
      301 tttgtggcgg tggcggaggc gctgtcgctg accaaagcgg aaattgtgac gctggcgaag
      361 aacagcttcc tcggctcgtt tttgagcgag actgaaaaag cgcaccacct cgcggaggtg
      421 gccgcgtatg tggcggcgcg gggcatcgct gagaattaga aacaatccgg tttgctacgc
      481 tccatcttcg tccgagtgtt tttgtttcgt cactgcgatt gtgctaaaaa aaccgatcga
      541 atgttctcgg ggaggacgcc gtgtcgaagc ctttcaccat catttccgcg ctgttgcttc
      601 ttgcgatcgc cgccgcgcac gcctaccgcg cttattacgg tatcgacgtg ctcgtcgcgg
      661 gtcatgccat tccgatttgg gcaagctggg tcagcaccgc ggtcgcggcg gtcttgggga
      721 tcatgctgtt cgtcgaagcg ccgcgccgca aataacggcg gttacgacgc gaacaaattc
      781 gttcatgcgt taagcgcgcg atgccgtcgc gttgcgccgg atcgcgctag ggatgcagtg
      841 cggccaccgc gacgccgatg tggtacggcc agatcaggat ggccaacgcc ccgggccaga
      901 aggcaagctt caaatacccg acggtgaaca gccaaccggc cagccagaac agccccgctc
      961 cctccgcgtg cacggtgaca tgtgattttt ccatgcccca gcatagccgc aggacacttt
     1021 cccgcccagt gtcgcggctg catcagccgg gaatagtcac gcggagctgc gaagaccgcg
     1081 gagcatcatt atttatcttg atttatcttg tcatccccgg tcgaacggcg ccttggcgcc
     1141 gggccatagc gtcagcgtat ggccggaccc aggtggtgca agcggcacag tctctccgtt
     1201 caggcaaagt tattggcacg cggagacgcg gagcgggcgt tccagatttc ctcttcgcgt
     1261 ctccttctgc cgtagtgcaa gaatcggctt cgccggaaaa ggcgatgtcc gtgaagcaga
     1321 acacggcacc cccgcgtctc cgcgtgaatc atctaggaag atcggggtca gagcagacac
     1381 ccgctatttc gccgtcactt catattcgac cggcacccag agaaagccgt ccccgtcttt
     1441 gcggatatgt ccgatgccgg ggaagggcag atgggaaccc gcgatcgccg tgccgtcttt
     1501 tgccaggctg gcgaacagcg tccggcgggt ggcggcggcg gccgcagcat cggtgtcgga
     1561 agagattgtc acgtccggaa gcgggaactg cacggcatag gaatgcacga tgtcgcccat
     1621 cgccagcagc gcctgcccct tggattcgaa gaggtatgcc gtctggcccg gcgtatgccc
     1681 ggggatggga acgctgcgca ggcccggcac aatggtctcg cccgctttga agcggtggaa
     1741 ggcgcccgcc gcgcggtaaa tatccatcac ctcgcgcgcc gtcttgaagg gcgcacgcgc
     1801 cttctctggc atggtgtcga ggtcgctgcc gagccaatag gccgcttctt cttcggccac
     1861 ccagaccgcg gccttgggat aggcgcgcgt gccgtccgca ttcttgagcc cgc
//
LOCUS       DASWPU010000281         2751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_100592,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000281 DASWPU010000000
VERSION     DASWPU010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100592"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VGC27_05975"
     CDS             complement(<1..666)
                     /locus_tag="VGC27_05975"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY0282152.1"
                     /translation="MANPILVEITRGALVESVHRGSIAIADAEGAIRFALGDIETPVY
                     PRSALKPIQALPLIESGAAEAFGVNDAEVVLACASHSGEPMHTQRIAGWLARIGCSET
                     DLACGPQVPRHIPTSEAMARHGNKPGRIHNNCSGKHAGFITLARYLNVAVAGYERVDH
                     PVQQAVATSLRALSGVSELPWGIDGCAAPNFALPLAGFARALAKLAGGRTKGAARILR
                     SMIA"
     gene            848..1378
                     /locus_tag="VGC27_05980"
     CDS             848..1378
                     /locus_tag="VGC27_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0282153.1"
                     /translation="MRGYFAAGVDGISKPMNLGNLLRIAHAFDASFFFSINPRLKLSD
                     AQSDTSRAHGSLPVYNFPTVEEFRLPIGCRLVGVEITDEATELPRFRHPQCAAYVFGG
                     ERGSLSEAILKRCEFVVKIPTRFSINVGMAGAIVLYDRLISLGRYPDRPVAPGRGSDI
                     PPPHKWGAPLARTLRR"
     gene            complement(1588..2589)
                     /locus_tag="VGC27_05985"
     CDS             complement(1588..2589)
                     /locus_tag="VGC27_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0282154.1"
                     /translation="MAAKHLETAVKPIRIGILGAARIAPKAVIVPARDNPEFVVAAVA
                     ARDRSRAEAYAAEHGIPSVADGYAALIASPDVDLVYVALPPAAHLAACVEAAKAGKAV
                     LCEKPFAMNAAEARAMVSAAAEAKRPLIEAFHNRFHKVMHRARAIVASGELGPLLEAD
                     AVFDTEIAYTSTELRWIPAQGGGALMDLGCYCVHALRTLTACEPKVASAGCTVVRDVD
                     ETTFAELLFPNGLRAKLRTSMKAPKFVATLRLKGEKGSLDISNYLAPQMGCRFKVEVQ
                     GKTRDEAVDGPTTYDAQLANVADVMLRGAAPLTGGADAIANMACIDAIYAAAGHGRT"
BASE COUNT          463 a          914 c          872 g          502 t
ORIGIN      
        1 ggcgatcatc gaacgaagga tgcgggccgc gcccttggtc cgtccgccgg ccagcttcgc
       61 cagcgcgcgc gcgaaaccgg cgagcggcaa ggcgaagttc ggcgccgcgc agccgtcgat
      121 tccccagggc agttcgctga cgccggacag cgcgcgcagc gatgtggcca ccgcctgctg
      181 gacgggatga tcgacccgct catatcccgc cacggccacg ttcaaatacc gcgccaaggt
      241 tatgaagccg gcatgcttgc cggagcaatt gttgtggatt cgtccgggct tgttgccgtg
      301 tcttgccatc gcctctgaag tcggtatgtg gcgcggaacc tgggggccgc aagccagatc
      361 ggtttcgctg caaccgatcc gcgccagcca acccgcgatg cgctgcgtat gcatcggctc
      421 gccggaatgg gaggcgcagg ccagcaccac ctctgcgtcg ttcaccccga acgcctccgc
      481 ggcccccgat tcgatcagag gcagcgcctg gatcggtttg agtgccgagc gcgggtaaac
      541 cggcgtctcg atgtcgccca gggcgaagcg aatcgcgcct tcggcatcgg cgatggcgat
      601 ggagccgcga tggacgcttt ccaccagcgc gccgcgcgtg atttcgacaa ggatgggatt
      661 ggccattcgg tctctctggt gccacccttc gacccgccta cgctaaagct tcggcggact
      721 agctatccag cacaagtccg ccatagcata gcgaaggcgg acaagctcag gatgagggct
      781 tatctatgct ccctcatgct gagcttgtcg aagcatgaga ggtatgtgca agcggcggga
      841 ctaagacatg cgcggctatt tcgcggcggg cgtggacggc atctccaagc cgatgaatct
      901 cggcaatctc ctgcgcatcg cccatgcctt cgacgcaagt ttcttcttct cgatcaatcc
      961 ccggctcaag ctgtccgacg cccagtccga cacctcgcgc gcccacggat cgctgccggt
     1021 ctataatttc cccacggtcg aggagttccg cctgccgatc ggctgccgtc tggtcggcgt
     1081 ggagatcacc gacgaggcga ccgagctgcc gcgcttccgc catccgcaat gcgcggccta
     1141 tgtcttcggc ggcgagcgcg gttccttgtc cgaagcgatt ctcaagcgct gcgagttcgt
     1201 agtgaagatc ccgacgcgct tctcgatcaa tgtcggcatg gcgggggcca tcgtgctcta
     1261 cgaccgcctt atcagcctcg gccgctatcc cgaccggccg gtggcgccgg gacgcgggtc
     1321 cgacattcct ccgccgcaca aatggggcgc gccgctggcg cggacgctgc ggcggtagag
     1381 caagttgggg attatggaaa atcagaaact catttgtcat ggccagctac tgcgggccat
     1441 ccggttgaaa cctgttgaaa tagtcaaaga agaagcagcg ggcttccttg ggctctgagc
     1501 tggcgtcacc tgggtggccc gcataaaagc gggccatgac atcgaggttt cttgcattac
     1561 cttgatgcca aacgacttaa tcttccatta cgtccgcccg tgccccgccg ccgcatagat
     1621 cgcgtcgatg cacgccatat tggcgatggc atcggcgccg cccgtaagag gcgccgcgcc
     1681 gcgcagcatc acgtcggcga cgttggcgag ctgggcgtcg taggtcgtcg gcccgtccac
     1741 ggcttcgtcg cgcgtcttgc cctggacctc caccttgaag cggcaaccca tctgcggcgc
     1801 gagataattg gagatgtcga gcgatccctt ctcgcccttc agacgcagcg tcgcgacgaa
     1861 tttcggcgcc ttcatcgagg tgcgcagctt ggcgcgcagt ccgttcggga agaggagttc
     1921 cgcgaacgtg gtctcgtcca cgtcgcgcac gacggtgcag ccggcgctcg cgaccttcgg
     1981 ttcgcaggcg gtcagcgtgc gcagcgcatg gacgcaatag cagccgaggt ccatcagcgc
     2041 gccgccgccc tgcgccggaa tccagcgcag ttccgtcgac gtataggcga tctcagtgtc
     2101 gaacaccgcg tcggcctcga gcagcggtcc tagttcgccc gaggcgacga tggcgcgggc
     2161 gcggtgcatg accttgtgaa agcggttgtg gaacgcctcg atcagcggtc gtttggcttc
     2221 cgcggccgcc gaaaccatgg cccgcgcctc ggcggcgttc atcgcgaacg gcttttcgca
     2281 cagcacggcc ttgccggcct ttgccgcctc gacacaagcg gccaggtgcg cggcgggcgg
     2341 taacgccacg taaacaaggt cgacgtccgg actcgcgatc agcgcggcat agccgtctgc
     2401 gacggaagga atgccgtgct ccgccgcata ggcttcggcg cggctcctgt cccgcgcggc
     2461 aaccgccgcg accacgaact ccggattgtc ccgggccgga acgatgacgg ccttgggcgc
     2521 gatgcgtgcc gctccgagaa ttccaatgcg aataggtttc acggcggttt ccaaatgctt
     2581 cgcggccatg atactcactg cggcccatga ctccagccct gtccatctcg gctctgcgca
     2641 aggtctatcg ccgcggcacc gtggccgtgg acggcctgtc catgaccatc gcggagggcg
     2701 atttcttcgg ctttttgggg cccaacggcg ccggcaaatc caccaccatc c
//
LOCUS       DASWPU010000282         5919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_2264, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000282 DASWPU010000000
VERSION     DASWPU010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1665)
                     /gene="glnA"
                     /locus_tag="VGC27_05990"
     CDS             complement(256..1665)
                     /gene="glnA"
                     /locus_tag="VGC27_05990"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY0282155.1"
                     /translation="MANAQSVLSTIKAKDVKFVDWRFTDPRGKWQHVTFDLSQIDEET
                     LTNGLMFDGSSIAGWRTINDSDMTLVPDCSTAVIDPFFAQTTLAIFCDVIVPATGQHY
                     ERCPRSIAKAAEKYMISTGIADTAFFGPEAEFFIFDDVRFDVQMNKCFYYLDSVGGPY
                     NSGTEYEGGNLGHRPAVKGGYFPVPPVDGYQDIRSEMLSIMGDLGIQPEKHHHEVAGS
                     QHELGCKFNTLVKGSDNLQIYKYVVHQVAAAYGKTATFMPKPVAGDNGSGMHVHQSLW
                     KGGKPLFAGSGYADLSDTCLYYIGGIIKHAKAINALTNSLTNSYKRLIPGFEAPVLLA
                     YSARNRSASCRIPFSASPKGKRVEVRFPDAGSNPYLGFSAMLMAGLDGIENKIHPGDP
                     VDKDLYHLPPAELKEIPTVCGSLRQALENLDADRAFLKKGGVFSDDFIDSYIELKMEE
                     VYNFEHTPHPIEFKMYYSV"
     gene            complement(1716..2054)
                     /locus_tag="VGC27_05995"
     CDS             complement(1716..2054)
                     /locus_tag="VGC27_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY0282156.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQEVGVQGITVTEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEIVIGEDRLQASIDAIQKAARTGRIGDGKIFILNVEEAIRI
                     RTGETGADAI"
     gene            2391..2939
                     /locus_tag="VGC27_06000"
     CDS             2391..2939
                     /locus_tag="VGC27_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0282157.1"
                     /translation="MGRLYLVRNARTQDGAGFDANPGLDDVGREQVEAVVMKLGSLPR
                     MRVLTSPQKRAIETAQPLARHWGANPIVDEAVTLMPLPDAETCDRRTWLLDFMKGSWR
                     DAPLQQTQWRENCLAALARQTDDIVIVSHFIVINMIVGAAAGDDRVVVFQPDNGSITT
                     MEASKGKLSLVDRGRQAATRPL"
     gene            3005..4480
                     /locus_tag="VGC27_06005"
     CDS             3005..4480
                     /locus_tag="VGC27_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY0282158.1"
                     /translation="MSNEIVSVAEMYAADRFASEHGVPSLTLMENAGRAVADEIVKRW
                     SPRPTAVLCGPGNNGGDGYVVARLLKARGWDVWVETLAPRETLHGDAAEMARQWTGET
                     MAVASANREPGLFVDAMFGAGLSRPLGGEAGRLAAQSPQFAERVIAIDVPSGLHGDTG
                     RGLGGVAVHAALTVTFFRKKPAHVLMPGRIDCGEIVVADIGIPDAALEAAPPRLFENG
                     PGLWGDDYPRPRPLAHKYARGHALIVSGPAHATGAARLAARGALRIGAGLVSVASPPD
                     AVGINAAQLTSIMVKPFADAAALSGLLADKRFNAAVIGPGCGVGQKTRELVAAVMKSG
                     AAAVLDADALTSFAAEPQTLFRLLRPSAVLTPHEGEFEQIFPGLLARSPTRIEAVREA
                     VGIAGCVVLLKGPDTAIAAPDGCVCISTNAPPWLATAGAGDVLAGFIAGLLAQGMEPF
                     KAAAAAAWLHGEAANLVGPGLISEDLPEQMPALLRRLYNGL"
     gene            4482..5105
                     /locus_tag="VGC27_06010"
     CDS             4482..5105
                     /locus_tag="VGC27_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0282159.1"
                     /translation="MQPKWLLWARELQAAAQNGLTYSENPYDRARYAAMQALAARIMA
                     EGSGTDLAVIEGLFAQQAGYATPKVDVRGAIFRERSVLLVSETIDGGRWTMPGGFADV
                     NEAPSVSLVREVREEAGYVVTARKLAAVYDRDKHGHPHPHPFHIYKLFFICDIVGRCE
                     KSDLETGEPQFFPLDALPELSESRTLPWQIQRMYEHHLSPSLPTDFD"
     gene            5121..5624
                     /locus_tag="VGC27_06015"
     CDS             5121..5624
                     /locus_tag="VGC27_06015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0282160.1"
                     /translation="MTEIVVRRVEQDDLPALLAIYNHYIVHTPVTFDLEPRTPAQRQA
                     WLDTFAPAGRHQCFVAARDGKAIGWACSGSFKEKAAYDISVETSIYLAPGEQGKGLGR
                     RLYQTLFDALAGEDLHGAFGCITLPNEASIGLHLAMGFSYVGTFREAGRKFGRFWDVA
                     WYGRAIP"
BASE COUNT         1049 a         1766 c         1958 g         1146 t
ORIGIN      
        1 tcttttcggc actgatctgg tcgccggtat tgctattttc cggttacctg ttgggcgaca
       61 cgctcgcaag cggctggagc atgcaggtga aagtggcggt aatcttgtcc gccattgggt
      121 tgatcgtttt ggggattttc ggcgcacggc gtctgttcca cgtgaggtga ccatatcggt
      181 gaaacccgac gaaaaaggcc ccggtgtttc cgccggggcc tttcgtgctt ctgacttggg
      241 gagagaaggc cggacttaga ccgaataata catcttgaac tcgatcggat gcggcgtgtg
      301 ctcgaagttg tacacttctt ccatcttgag ttcgatgtag ctgtcgatga agtcgtcgct
      361 gaagacgccg cccttcttga ggaaggcgcg gtcggcgtcg agattctcca gcgcctggcg
      421 caggctgccg cagacggtcg ggatttcctt cagctccgcc ggcggcaggt gataaaggtc
      481 cttgtccacc gggtcgccgg gatggatctt gttctcgatg ccgtcgaggc ccgccatcag
      541 catcgccgaa aacccgagat agggattgct gccggcatcc gggaaccgca cttccacgcg
      601 cttgcctttg gggctcgccg agaacggaat gcggcaggag gccgagcggt tgcgcgccga
      661 ataggcgagc agcaccggcg cttcgaaacc ggggatcagc cgcttgtagg aattggtcag
      721 cgagttggtc agcgcgttga tcgccttggc gtgcttgatg atgccgccga tgtaatagag
      781 gcaggtgtcg gacagatcgg cgtagcccga gccggcgaac agcggcttgc cgcccttcca
      841 cagcgactgg tggacgtgca tgccggagcc gttgtcgccc gcgacgggct tcggcatgaa
      901 ggtcgccgtc ttgccgtagg ccgcggccac ctgatgcacg acgtatttgt aaatctgcaa
      961 attgtcgctg cccttgacca gagtgttgaa cttgcagccc agttcgtgct gcgagccggc
     1021 cacttcatgg tgatgcttct cgggctggat gcccaggtcg cccatgatgg agagcatctc
     1081 gctgcggatg tcctggtagc cgtccaccgg aggaaccggg aaatagccgc ccttgaccgc
     1141 cggacgatgg cccaaattgc cgccttcgta ttcggttccg gaattgtaag ggccgccgac
     1201 cgagtcgagg taatagaagc acttgttcat ctggacgtcg aaacggacgt cgtcgaagat
     1261 gaagaattcc gcctcggggc cgaaaaaggc ggtgtcggcg atgccggtcg agatcatgta
     1321 tttctcggcg gccttggcga tcgagcgcgg gcagcgctcg taatgctggc cggtggcggg
     1381 aacgataacg tcgcagaaga tcgccagcgt cgtctgcgcg aagaagggat cgatcacggc
     1441 ggtgctgcaa tcgggcacca gcgtcatgtc ggaatcgttg atcgtgcgcc agccggcgat
     1501 cgacgagccg tcgaacatca gaccgttggt gagtgtctct tcatcgatct gcgagaggtc
     1561 gaaggtgacg tgctgccact tgcctcgcgg atcggtgaat ctccagtcga cgaatttgac
     1621 gtccttggcc ttaatcgtcg acaggacgct ttgtgcatta gccatagtct cggtctttcc
     1681 cttctatgtt gagattgtgt tccctcaggc agccgctaaa tggcgtctgc gcctgtctcg
     1741 ccggtacgaa ttcgaattgc ttcttcgaca ttgaggatga aaatcttgcc gtcgccgatg
     1801 cggccggtgc gcgcagcttt ctggatggcg tcgatcgacg cctgcagacg gtcttcgccg
     1861 atcacgattt cgattttcac cttgggcagg aaatcgacga catattcggc gccgcggtac
     1921 agttcggtat gtcctttctg acggccgaag cccttggcct cggtcaccgt gatgccctgg
     1981 acaccgacct cttgcagcgc ctctttcact tcgtcgagct tgaatggctt gatgatggcc
     2041 tcgatcttct tcattgcgtg ctccttgcgc ggctctagat accgcgcggt taacgaagta
     2101 gcagggcttg tgccactttc ccaggactta atcacaagta atttcagcag cttataacgc
     2161 ggcaccttgg ctggcggtaa aacatgctta gaaaaaagtc atattgcatt ataaacaggc
     2221 actccttcgg aggcgagtca ttgtcagcgc cgacctgctt ttgcgatggt acgcatggct
     2281 ggcgaatccg agggtcaatg tcgcatacgc attgccttct gcaagaagcg cgaagtgatc
     2341 gaagagacgc tccggcgtct cgactgctat ttcaggatcg ggagggattt atgggccgtc
     2401 tctatttagt gcgcaacgct cgcacgcagg acggggccgg cttcgatgcc aaccccggac
     2461 ttgacgacgt cggtcgagag caggtggaag ccgtcgtcat gaagctcggt tcgcttccgc
     2521 gcatgagagt tctgacgagc ccgcagaagc gcgcgattga gacggcgcag cctctggcgc
     2581 ggcattgggg ggcgaaccct attgtcgacg aagccgttac cttgatgccg ctgcccgatg
     2641 cggaaacgtg cgatcgcaga acctggcttc tcgatttcat gaagggttcc tggcgcgatg
     2701 cgcctttgca gcagacacaa tggcgggaga actgccttgc cgcgcttgcc cgccagacgg
     2761 acgacatcgt catcgtttcg catttcatcg tcatcaacat gatcgtcggc gcggcggcgg
     2821 gggatgatcg tgtggtcgtt ttccagccgg ataacggttc aatcacgact atggaagcgt
     2881 cgaagggcaa gctgtcgctg gtggatcgcg gccgtcaggc ggcaacgcgg ccgctctgat
     2941 gtccgcccgc aaccttagac gggcctgaaa atgaggtata gcttcgtcat accggggccg
     3001 catcatgagc aatgagatcg tcagcgtcgc cgaaatgtac gcggccgacc gcttcgcctc
     3061 cgaacacggc gtgccgtcgc tgaccttgat ggagaacgcc ggccgcgccg tcgcggacga
     3121 gatcgtcaag cgctggtcgc cgcgcccgac ggcggtgctg tgcggacccg gcaacaatgg
     3181 cggcgacggc tacgtcgtcg cgcggctgct caaggcgcgc ggctgggatg tctgggtgga
     3241 gacgctggcg ccgcgcgaga ctctgcacgg cgacgcggcc gagatggcgc ggcaatggac
     3301 gggcgagacg atggcggtcg cgtccgccaa tcgcgagccg ggacttttcg tggacgccat
     3361 gttcggcgcg ggactgtcgc ggccgctggg cggcgaagcc gggcgtttgg cggcgcagtc
     3421 gccgcaattc gccgagcgcg tgatcgccat cgacgttccc agcggcctgc atggcgacac
     3481 gggccgcggt ctgggcggcg tcgccgtcca tgcggccttg accgtgacgt tcttccgcaa
     3541 gaaaccggcg cacgtcctga tgccgggcag aatcgattgc ggcgagatcg tcgtcgccga
     3601 tatcggcatt cccgatgcgg cgctcgaagc cgccccgccg cgcctgttcg aaaacggacc
     3661 cggcttgtgg ggcgacgatt atccccggcc gcgacccctt gcccataaat acgcgcgcgg
     3721 ccatgcactg atcgtcagcg ggccggcaca cgcgaccgga gcggcgcggc tggcggcgcg
     3781 cggcgcattg cgcatcggcg cgggtctggt gagcgttgca agtccgcccg atgccgtggg
     3841 gatcaatgcg gcgcagctga cgtccatcat ggtcaaaccg tttgcggatg cagcggcgct
     3901 gtccggcctt ctggcggaca aacggttcaa cgccgccgtt atcggtccgg gctgcggcgt
     3961 ggggcagaag accagggaac ttgtcgccgc ggtgatgaag agcggcgcgg ccgcggtgct
     4021 cgacgccgat gcactgacct ccttcgccgc cgagccgcag acgctgttcc gtctgctgcg
     4081 cccgtcggcc gtgctgacgc cgcatgaagg cgagttcgag cagattttcc ccggccttct
     4141 cgcgcgttcg ccgacccgga tcgaagcggt gcgggaggct gtgggaatag cgggctgcgt
     4201 tgtgctcctc aaagggccgg atacggcgat cgcggcgccg gacggctgtg tctgcatctc
     4261 gaccaacgcg ccgccatggc tcgccacggc aggggcgggc gacgtgctcg ccgggtttat
     4321 cgccgggcta ttggcgcagg ggatggagcc gttcaaagcc gccgccgctg cggcatggtt
     4381 gcacggcgag gcggcaaatc ttgtcggacc ggggttgatc tcggaggatt tgccggaaca
     4441 aatgcccgcg ctgctgcggc ggctctacaa cggactctga catgcaaccg aaatggcttt
     4501 tgtgggcgcg cgaactgcaa gcggcggcgc aaaacggcct cacctacagc gagaatcctt
     4561 acgaccgcgc gcgctatgcg gccatgcagg cgttggccgc gcggatcatg gcggaaggtt
     4621 cgggcaccga cctcgccgtc atcgaagggc tgttcgcgca acaagccggt tacgccaccc
     4681 ccaaagtgga cgtgcgcggg gcgatattcc gcgagcgcag cgtccttttg gtcagcgaaa
     4741 ccatcgacgg cggccgctgg acgatgccgg gcggcttcgc ggacgtgaac gaggcgccca
     4801 gcgtgagcct ggtgcgggaa gtgcgcgagg aggcgggata tgtggtgacg gcgcgcaagc
     4861 tagcggcggt gtacgaccgc gacaagcacg gccatccgca cccgcatccg ttccacatct
     4921 acaagctgtt ctttatttgc gacatcgtcg ggcggtgcga gaaatcggac ctggaaaccg
     4981 gcgagccgca attcttcccg ctcgacgctt tgccggaact gtcggagtcg cgcacgctgc
     5041 cgtggcaaat ccagcgcatg tacgaacatc atctgtcgcc gtcgctgccg acggacttcg
     5101 attaagaaag agcgcttccc atgaccgaga tcgtcgtccg ccgcgtcgaa caggacgatc
     5161 tgccggcgct gctcgccatc tacaatcact acatcgtcca cacgccggtg accttcgacc
     5221 tggagccgcg cacgccggcg cagcgccagg catggctcga cacgttcgcg cctgcggggc
     5281 ggcaccaatg cttcgtcgcc gcgcgggacg gaaaggcgat tggctgggcc tgttcgggca
     5341 gcttcaagga gaaggccgcc tacgacatct cggtggagac gagcatctat ctggcgccgg
     5401 gcgaacaggg gaaggggctg ggccgcaggc tgtaccagac tctcttcgac gccctcgccg
     5461 gcgaagacct gcacggcgcc ttcggctgca tcacgctgcc gaacgaagcg tccatcggcc
     5521 tgcatctggc gatgggtttt tcctatgtcg gaaccttccg cgaagccggc cgcaaattcg
     5581 gccggttctg ggatgtggcg tggtacggcc gggcaatccc atagagcgct tcccgaaaag
     5641 tgggacccgg ttttcggata agaagcgcga caaaacaaat agctggagca aatcactgat
     5701 tccaataaac ggtaatttgc tctagaaccg ccggcatggc tgcctgcgtc atccggcatg
     5761 gctgcctgcg tcataaggca cataagggcc gggccgcggc gggggaaatg tccggttacg
     5821 aggtctagcg gcgggccaaa tcccctgcta tagcggcccc cttccgctgg gaccggttca
     5881 gacggcacgg cggaatagcc cgcgcgcggg cgtggcgga
//
LOCUS       DASWPU010000283         4178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_21076,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000283 DASWPU010000000
VERSION     DASWPU010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21076"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..2983
                     /locus_tag="VGC27_06020"
     CDS             77..2983
                     /locus_tag="VGC27_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HEY0282161.1"
                     /translation="MRRLILAAMAAAFLVGAPAASNPAPAPHSANFGGPEDVIELKQT
                     LGPYKSAPATKATASEWYTFTAVNESNRPAIRVLHAGQPPDIGLGVLPRASRPTVMQV
                     VSTNPGVIVEGNNAYGRHAFRVSIPAQTSAPLAVRIVGAQSPPSLLAWTEPALAAHNR
                     QLAIFVAAVAALIGAAAAIAAGLAVMTHHGPPRWAALTLLLMLLTRLSLVGMFDGSLV
                     TSVGGPYGLIALFAGLTLAAGVRLVDMIVPLQEIWPRAQRWFGFVVPGVMTLSALAYL
                     GVPGATLLTDIVLVCGTLAIAAYLVFRGRGGSKTARVLTPSAGLFALVALAATFSALG
                     VFGNTPLVPDVAGGFAAAGAVLLVLAVATGEGIAVLPFTRPVHAAALPPMPQQAPPPA
                     PPPAAGPHPAVEAIGASHQGVFELDFAEEAVVLSREAAALIGLEAGRMAHVLWVARVH
                     PDDRDVYEQAISDFSAQSGVAFRIEFRVRTEGGGYLWLELRATMKGPENRAAERCVGL
                     LADITIRKDAEAAMMDRTLRDPLTGLGNRVALMEELESLGERLHDATFAMLDVDRFKT
                     IHASLGDDGGDQVLSNLAQRLSKRFFGIAQVFRVGGDGFAALFRIPSGTPEEVGAELV
                     EVCGAVHQLDGRNVFAPASVGIAEGREARDPLDLLKNAELALMRAKRQGGDCARVYTR
                     ELEAVAPADAVTLEAELRRAIGNDEIEVFYQPIVRLADRTVAGFEALLRWRHPVRGLV
                     APADFIAHSEETGLIVALGRLALQRAAKDLAQLQRFFPLPEALFVSVNLSRRQLLDLD
                     LETFLAELLSGAAIESGTLKLEVTESAVAADADACALMTRIRALGAGLAIDDFGTGLS
                     SLSQLRDLPFDTVKIDKSFLSRHGGTDADCDADVVLSSIVALARDLKRGVIVEGVETE
                     RDAERMLEFGCEYAQGFLFSEPLTAKDALAYIARHYRVGTDGWRSGAAGLGGQA"
     gene            complement(2951..3559)
                     /locus_tag="VGC27_06025"
     CDS             complement(2951..3559)
                     /locus_tag="VGC27_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY0282162.1"
                     /translation="MLTLRMAHSAPRSQKSGESFLEGKLLIAMPGMPDPRFEKSVIFM
                     CAHSAEGAMGLIINKPIDGLSFGELVDKLGIGMKVARSDQPILFGGPVQMGRGFVLHS
                     ADYTSEDSTLTLTPEISLTATVDILRAIAEGRGPEKAVLALGYAGWGEGQIEAEILSN
                     GWIHCDADSGLVFDTDYESRWQKAFARLGADISGLSAEAGRA"
     gene            3626..4108
                     /locus_tag="VGC27_06030"
     CDS             3626..4108
                     /locus_tag="VGC27_06030"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0282163.1"
                     /translation="MTIQIGGKVPSATLMQMKDGSPQPVKTDELFAGKKVVVFALPGA
                     FTPTCSAKHLPGFVRHAEEIKRKGIDAIACIAVNDVFVMNAWGEQQGAGGKVMMLADG
                     NGELTAALGLEMDAAKYGMGKRSQRYSMLVEDGVVKQLNIEEPGAFAVSSAEHILGQI
                     "
BASE COUNT          642 a         1342 c         1442 g          752 t
ORIGIN      
        1 gccgatatag agagggagcg aatttcggaa tcgggacact agcgccccgc aacacggacg
       61 atcatagacg cctgtgatga gacgactgat cctagccgct atggcggccg ctttcctggt
      121 tggcgcgcct gcggcgtcca acccggcgcc ggcgccgcat agcgcaaatt tcggcggacc
      181 ggaagacgtc atcgagctta aacaaacgct cggtccctac aaatctgcgc cggccaccaa
      241 ggccacggcg tccgaatggt acaccttcac ggcggtcaat gaatcgaacc ggccggcgat
      301 ccgggttctg catgccggcc agccgccgga tatcggcctc ggcgtgctgc cgcgcgccag
      361 ccggccgacc gtcatgcagg tggtcagcac caatcccggc gtcatcgtcg aaggcaataa
      421 cgcctatggc cgccatgcct tccgggtatc gattccggcg cagacctcgg cgccgctggc
      481 ggttcgcatc gtgggagcgc aatcgccgcc gtcgcttctg gcctggaccg agcccgcgct
      541 tgccgcccac aaccgccagc ttgcgatctt cgtcgccgcc gtcgccgcgc tgatcggcgc
      601 cgccgcggcg atcgcggccg gtctggcggt gatgacccat cacggcccgc cgcgctgggc
      661 ggcgctgacc ttgctcctga tgctgctgac gcggctctcc ctggttggga tgttcgacgg
      721 cagcttggtc accagcgtag ggggaccgta cggcctgatt gccttgttcg ccggattgac
      781 cctggcggcg ggtgtgcggc ttgtcgatat gatcgtcccg ctccaggaaa tctggccgcg
      841 agcgcagcgc tggttcggct tcgtcgtgcc cggcgtgatg acgctgtcgg cgctggccta
      901 tttgggcgtg ccgggcgcga ctttgctgac cgacatcgtt ctggtctgcg gcacgctggc
      961 gatcgccgcc tatctggttt tccgcgggcg cggcggttcc aagaccgcgc gcgttctcac
     1021 gcccagcgcg ggattgttcg cattggtggc cttggccgcg accttttcgg cgcttggcgt
     1081 cttcggcaat acgccgctgg tgccggacgt ggcgggcggc ttcgccgcgg cgggcgcggt
     1141 gctgctggtg ctggccgtcg cgacgggcga ggggattgcg gtgctgcctt tcacccgtcc
     1201 cgtacatgcc gcggcgctgc cgcctatgcc gcagcaggcg ccgccgcccg ctcctccgcc
     1261 ggcggcgggt ccgcatccgg cggtcgaggc catcggcgct tcgcatcaag gcgtcttcga
     1321 gttggacttc gcggaggaag ccgtggtgct gtcgcgcgaa gcggcggcgc tgatcgggct
     1381 tgaggccggc cgtatggcgc atgtgctttg ggtggcgcgc gtgcatcccg acgaccgcga
     1441 cgtttacgag caggcgattt ccgatttcag cgcccagtcc ggggtggcgt tccgcatcga
     1501 gttccgggtg cgcaccgaag gcggcggcta tctctggctc gaactgcgtg cgacgatgaa
     1561 gggccccgag aaccgggccg cggaacgctg cgtgggcctg ctggccgaca ttaccatccg
     1621 caaggacgcc gaagcggcga tgatggaccg caccttgcgc gatccgttga cggggctggg
     1681 caaccgcgtg gcgctgatgg aagagcttga gagcctgggc gagcggctgc acgacgcgac
     1741 cttcgccatg ctcgacgtcg accgcttcaa gaccattcac gccagcctcg gcgatgacgg
     1801 cggcgatcag gttttgtcga acctggcgca aaggctgagc aagcggtttt tcggcattgc
     1861 gcaggttttc cgtgtcggcg gcgatggctt tgcggcgctg ttccggatac cgagcggcac
     1921 gcccgaggaa gtcggcgccg agctggtgga ggtttgcggt gcggtgcatc agcttgacgg
     1981 gcgcaacgtc ttcgcgccgg ccagcgtcgg gatcgcggag ggccgcgagg ctcgcgatcc
     2041 gctcgatctg ttgaagaacg ccgagctggc gctgatgcgg gccaagcggc agggcggcga
     2101 ctgcgcccgg gtgtacacgc gcgagcttga ggccgtggct cccgccgatg ccgtgacgct
     2161 cgaggccgag ctgcgccgcg ccatcggcaa cgacgagatc gaggtttttt atcagcccat
     2221 cgtcaggctg gcggaccgca ccgtggcggg gttcgaagcg ctgttgcgct ggcggcatcc
     2281 ggtcagggga ttggtggcgc cggccgactt catcgcccat tccgaggaaa ccgggctgat
     2341 cgtcgcgctg ggacggctgg cgctgcaacg cgccgccaag gatcttgcgc aattacagcg
     2401 cttttttccg ctccccgagg cgctgttcgt cagcgtcaat ctctcgcggc gccagcttct
     2461 cgatctcgac ctggaaacct tcctcgccga gcttctgtcc ggcgcggcga tcgagtcggg
     2521 cacgctgaag ctggaggtga cggaaagcgc cgtcgccgcc gacgccgacg cctgcgcgct
     2581 gatgacgcgg atccgcgccc tgggcgcggg tctggcgatc gacgatttcg gcaccggcct
     2641 gtcgagcctg agccagttgc gcgatctgcc gttcgatacg gtgaagatcg acaagagctt
     2701 tctgtcgcgc cacggcggca ccgacgcgga ttgcgatgcc gacgtggtgc tgtcctccat
     2761 cgtcgcgctg gcccgcgatc tcaagcgcgg cgtcatcgtc gaaggcgtgg aaaccgaacg
     2821 cgacgcggag cgcatgctgg agttcggctg cgaatacgcc caggggttcc tgttttccga
     2881 gccgctcacc gccaaggacg cgctcgccta catcgcgcgc cattatcgcg tgggaaccga
     2941 cggttggagg tcaggcgcgg ccggcctcgg cggacaggcc tgaaatatcc gcgcccagtc
     3001 tggcgaacgc cttctgccat ctcgactcgt agtccgtgtc gaatacgagt ccgctatcgg
     3061 cgtcgcagtg gatccagccg ttcgacagga tttcggcttc gatctgtccc tcgccccagc
     3121 cggcatagcc gagcgcgagg acggcttttt cggggccgcg gccttcggcg atcgcccgca
     3181 ggatatccac ggtcgcggtc agcgagattt cgggcgtgag ggtcagcgtg gagtcctcgc
     3241 tggtataatc ggcgctgtgc aggacgaagc cgcggcccat ctgcaccggg ccgccgaaca
     3301 ggatcggctg gtcgctgcgc gccaccttca tgccgatgcc gagcttgtcc accagctcgc
     3361 cgaacgacag cccgtcgatc ggcttgttga tgatcaggcc catggcgcct tccgccgaat
     3421 gcgcgcacat gaagatgacg ctcttctcga agcgcggatc gggcattccg ggcatggcga
     3481 tgagcagctt gccctcaagg aagctctcgc cgcttttttg tgaccgtggt gccgaatggg
     3541 ccatgcgcaa cgttagcata acggcttcac cggcgcacgg cattaggtta gaactctttg
     3601 aatatatcac ggcaaggaga cgtccatgac catccagatc ggcggcaagg ttccatcggc
     3661 cacgttgatg cagatgaagg acggctcccc tcagccggtg aaaaccgacg agcttttcgc
     3721 gggcaagaaa gtggttgtat ttgcgctgcc gggcgccttc accccgacct gttcggccaa
     3781 gcacctgccg ggcttcgtcc ggcacgccga agaaatcaag cggaagggca tcgacgccat
     3841 cgcctgcatt gcggtcaatg acgtttttgt catgaacgcc tggggcgaac agcagggcgc
     3901 cggcggcaag gtgatgatgc tggccgacgg caacggcgaa ctgacggcgg cgctcggcct
     3961 ggaaatggac gctgcgaaat acggcatggg taagcgcagc cagcgctact cgatgctggt
     4021 cgaggacggc gtggtcaagc agctcaacat cgaggagccc ggcgcgttcg cggtgtcgag
     4081 cgccgagcac atcctggggc agatataagg ggcgaatgtt ttctgccccc acgaagtggg
     4141 ggaagcggcc gcgcagcggc cgaagggggg ttctgccc
//
LOCUS       DASWPU010000284         3213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_2271, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000284 DASWPU010000000
VERSION     DASWPU010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1128
                     /locus_tag="VGC27_06035"
     CDS             346..1128
                     /locus_tag="VGC27_06035"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEY0282164.1"
                     /translation="MRYQPLLARVGFGGFLLGLAVALTACFGTRLGFWDYAQGVRILV
                     PGVAVGVVALGCAAAWIARALAANNSLGWRLGSIGLIGSLLLVGIPADNLWRRMTLPP
                     IHDISTDIGDAPQFHTLLAWRKGAPNPASYDGQIIVSYGGERITTALAQKYAYADIKP
                     VERLKGRFTEKEFYGKMFWRALNTVNALGWQVASFDYKSGRIEATDTSFWFGTVSDIV
                     IRVRPAGAIGVRIDIRAKSRVGTSDAGRNAKLIRDFLPRMKT"
     gene            complement(1150..2244)
                     /locus_tag="VGC27_06040"
     CDS             complement(1150..2244)
                     /locus_tag="VGC27_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY0282165.1"
                     /translation="MADKLHQVYLARGALATTAIEGNTLSEGEAIAIVQEKSNLPKSQ
                     AYLEKEIKNILSASNAMLEKIERDGIAPITPNDIKQYNKTVLDGLEVAPHVTPGECVK
                     TDVEVAGYHGAPWQECEDLLAKLCAWLNGENFGSRDGDGIIPGILKAILAHLYLVWIH
                     PFGDGNGRTARLLEVRFLTEAGVPSAAGHLLSNFYNKTRTEYYRKLGEASKSGGNVIA
                     FLVYAVTGFVDELREQLKIVKNQQWYVSWQNYVYEAFEGRNSVSDKRQLKLALALTKQ
                     GGFVPKTELRHLTPALAEAYAGKTDKTLTRDLNELANLGLIERTKDGYRATKEIILAF
                     LPRGRKGSVEDQLRDASFFFEDGAQSALDL"
     gene            complement(2550..2861)
                     /locus_tag="VGC27_06045"
     CDS             complement(2550..2861)
                     /locus_tag="VGC27_06045"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEY0282166.1"
                     /translation="MRKFFLLALGWVLVVVGLVLTPAPIPIPLLGVIPLLAGCAILSA
                     NSKSFRRALQRLRLRFSFISRWLDGVRYRMPSGVKTMIRRTNPRALLRLARLRLRRRT
                     R"
BASE COUNT          656 a          948 c          908 g          701 t
ORIGIN      
        1 ataattataa tgtcacaacg ctctaatgtc gtggtttcga agttcgcagc atgcgcacta
       61 tcgggccgca agccaacttc gaaaccaaaa acgacactag gattcaataa gttgctagcg
      121 tcctttcgat tccgaaattc gcccgaagcc gatatagaga cggagcgaat ttcggaatcg
      181 ggacactagc gacggcttgg cgatgctctt gcggtccgcc gctcgttgtg gttttgacct
      241 cgcggcaaac ggcggcgaaa aaggatatac tgccccgcct tccggctgag atcggcaatt
      301 gcgggatgcc cggccgggca aaacataacg gatggcaggg ggaccatgcg ctatcaacca
      361 cttctcgcca gggtcggatt cggcggcttt ctgctgggac ttgccgtcgc gctgacggct
      421 tgcttcggca cgcggctcgg cttctgggat tatgcgcagg gcgtgaggat tctggttccc
      481 ggcgtggcgg tgggcgttgt cgcgctcggc tgcgccgccg cctggatcgc gcgcgcgctg
      541 gcggcgaaca acagcctggg ctggcggctg ggaagcatcg gcctgatcgg ctcgctgctg
      601 ctggtcggca ttccggccga caatctgtgg cgccgcatga cgctgccgcc gatccacgac
      661 atctccaccg acatcggcga tgcgcctcag ttccacacgc ttttggcctg gcgcaagggc
      721 gcacccaatc ccgcctctta tgacgggcag atcatcgtga gctatggcgg cgagcgcatc
      781 accacggcgc tggcgcagaa atacgcctat gccgatatca agccggtcga acgcctgaag
      841 gggcggttca ccgagaagga attctacggc aaaatgttct ggcgcgcgct caacaccgtc
      901 aacgcgctgg gctggcaggt cgcctcgttc gactacaaga gcgggcgcat cgaagccacc
      961 gataccagct tctggttcgg gaccgtgtcc gacatcgtga tccgggtgcg gccggccggc
     1021 gccatcggcg tgcgcatcga catccgcgcc aagagccgcg tcggcaccag cgatgccggg
     1081 cgcaacgcga aactgatccg cgacttcctg ccgaggatga agacgtaacg ctcggagcaa
     1141 gatgccatgt cagaggtcaa gcgctgattg cgcgccgtcc tcgaagaaga acgaagcatc
     1201 gcgcagttgg tcctctacgc ttccctttcg gccacgtggc agaaacgcca gtattatttc
     1261 ctttgtcgcg cgatagccgt ccttggttct ttctataaga cccaagttcg caagctcgtt
     1321 gaggtctcgc gtaagcgttt tgtccgtttt gcccgcgtac gcctccgcga gtgcgggcgt
     1381 taaatgccgt aattcagtct ttgggacaaa cccaccctgc tttgtgagcg cgagcgcaag
     1441 tttcaattgg cgcttatctg agacgctgtt tcgcccttca aatgcctcgt aaacgtaatt
     1501 ctgccacgac acataccatt gttggttttt gacaattttc agctgttccc ggagttcatc
     1561 tacaaaccct gtcaccgcat acacgagaaa cgcgatgaca ttgccgccgc tcttggatgc
     1621 ttcgccgagc tttctgtaat attcggttct ggttttgtta tagaaattgc tcaggaggtg
     1681 gcccgccgcg gatggcacgc cagcctccgt cagaaaccgc acttctagaa gtctggccgt
     1741 tcggccattg ccgtcaccaa acggatgaat ccaaacgaga tacaggtgcg ccagaatcgc
     1801 tttcaaaatg ccagggatga tgccgtcacc gtctcggctg ccaaagttct cgccgttcag
     1861 ccatgcgcac aacttcgcga ggaggtcttc gcactcttgc cagggagcgc catgataccc
     1921 ggcgacctcg acatccgtct tcacgcactc gcccggtgtt acgtggggcg caacctcaag
     1981 accatccaat acggtcttgt tgtattgctt tatgtcgttt ggcgtgattg gagcaattcc
     2041 gtctctttct attttttcaa gcatcgcgtt cgatgcactc aagatattct ttatttcttt
     2101 ttcaaggtaa gcctgtgact tcgggaggtt gctcttctcc tgaacgatag cgatggcttc
     2161 gccctcgcta agagtgttgc cttcaattgc agtcgtagct agcgcaccgc gcgccaaata
     2221 gacttgatgc agtttgtctg ccacctttgg cttaagtgga attccagaaa gatgctggca
     2281 cttagaccgc gcttcgccca aagctatcca caacaccgtg ggcgccggtc ggaggtccgc
     2341 cacaaaggtt atccaagggt gcgtccgctc ataagtcgtc atttttgtcc tgcatattat
     2401 actattttca cattataatc aaatggataa atattatccc gctatttatg tccatgtcaa
     2461 tgtccgcata tatgtctggg catatggacg gcttttatga tcacggattg tctatggaat
     2521 accgcatgcg ccacccccga aggcacccgt cagcgggtgc ggcggcgcag gcgcaggcgc
     2581 gccaggcgaa ggagcgcacg cgggttggtc cggcggatca tcgtcttcac gccggacggc
     2641 atgcggtacc gcacgccgtc cagccagcgt gatatgaagc taaagcgaag gcgcaggcgt
     2701 tgcagcgccc ggcgaaaact cttggaattc gccgacagga tcgcgcagcc cgccagcagc
     2761 gggatgacgc cgagcaacgg gatcggtatc ggcgcgggag tcagcaccaa gcccacgacc
     2821 accaggaccc atccaagggc gagaagaaaa aacttccgca tgcgccgaat ccagaccgga
     2881 ccgttaacta ccacaggtgg aatcatgcag gcgagttgcc gccggggaac gcagagcaca
     2941 actttagccg gagattaacg ccgcttcgcg ccgtatacgg cgctccacaa cgttaatcat
     3001 gctttcatca tttttgtact tctgcatgcc gatgtgccta acatagaggc atgcaggggc
     3061 ttgagcagac cacgaagagc aagcacacgg cgctcacgat ctggggcgtc tgcgtgtgtc
     3121 tgctgcttgg cccttccctg ctggtctgga tcgtgcgcgg cgccgccttc gcggtacaat
     3181 gccaacccgg acccgaactg tgtcacggca tga
//
LOCUS       DASWPU010000285         7979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_8368, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000285 DASWPU010000000
VERSION     DASWPU010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8368"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VGC27_06050"
     CDS             complement(<1..166)
                     /locus_tag="VGC27_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0282167.1"
                     /translation="MAHILLVEDDQSLRKFLAQALIRAGHDVTDFGDGEDAYECLADF
                     RFDLLLTDIVM"
     gene            342..1262
                     /locus_tag="VGC27_06055"
     CDS             342..1262
                     /locus_tag="VGC27_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY0282168.1"
                     /translation="MAGTGNEVESGGSDPILAELYREPFRIVLPREQTAPFVFASPHS
                     GRLYPADFVAKSNLAPLALRRSEDAFADELFESAVGRGAPMIAARFPRVFLDVNRAPS
                     ELDSAMFDGALHVEVGAPSTRVAAGLGVIPRVVRDGAEIYRGRLFPADAEKRLARLYR
                     PYHAALAGLIEKTRARFGVAILVDCHSMPSASSIPDIVLGDRYGVSASAALMRHTERA
                     FEACGFSTARNTPYAGGYTTLLYGRREQGVHALQIEVNRTLYLDEARIERLACFAETR
                     ARLSEALGKLVAMDTNLLMPNPPRQSLAAE"
     gene            1758..2681
                     /locus_tag="VGC27_06060"
     CDS             1758..2681
                     /locus_tag="VGC27_06060"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY0282169.1"
                     /translation="MAETAWSPNSFVRPEHAAPRSRRRPQAPSQAHYAAAHGHSHRTI
                     FISDVHLGTRGCKAELLADFLAHNDCNTLYLVGDILDGWQLKRRWYWSEAQSRVVSEI
                     LHKVDEGVRVVYVPGNHDEFLRAYCGRNIAGIEVVRETIHETAAGKRFLILHGDQFDG
                     VIGYAKWLAHVGDWAYSAALALNDRLHMLRSSLGLPYWSLSAYLKRKVKNAVEYVSRY
                     EEVVARAAASKGVDGVICGHIHQAEIRQIGPILYLNDGDWVESCTALVEDARGHLEIL
                     RWASCSPASDFARVSAGAKELVSTILAGRVS"
     gene            2988..4031
                     /locus_tag="VGC27_06065"
     CDS             2988..4031
                     /locus_tag="VGC27_06065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0282170.1"
                     /translation="MPTGIGTAEALSLKVLIVTDAWAPQVNGVVRTLEILGRDLQALG
                     HEVRYATPEGRFTLPLPTYSEIRLAVFPRTVLEREIREYAPDAIHIATEGTLGLTARA
                     ICVKYRIPFTTSFHTRFPDYVRARFPFIGEETVYRFLRWFHRPATAMMVATASLKREL
                     EDRGFHNVRIWSRGVDTDRFRPLPGAALPYPKPIWLYVGRVAVEKNIEAFLKLDLPGT
                     KVVVGKGPALTSLERAYPAAEFLGQKTGEALVEVYAASDVFVFPSRTDTFGLVVLEAL
                     ACGTPVAAYPVQGPKDVIDDARVAVLDDDLAKACREALSIPRDAARSFALSRSWRSCT
                     QQFLSNLAVEIEP"
     gene            4100..5698
                     /locus_tag="VGC27_06070"
     CDS             4100..5698
                     /locus_tag="VGC27_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282171.1"
                     /translation="MSSANPTAQSPRLAPRELAFLGLSILAWTAYVVWLGKDTSWDFR
                     NYHWYAPYAFLNGRMEIDVTVAHQASYYNPLADIPFYWLAIHTTSWFAIAVLGLFQAA
                     NIVPIYLIARHGLRHQMSELGSAAIAFFGLTGGLTLSLFGTHYYDNVLSVFTLGGIAI
                     LVVKREELARGSLKKAALWCALGGLMTGGAAGLKLPEAPFAMGFAAALIAVGGDRKHL
                     FTRLAAGALGGLAGFLLCAGFWMWRMEDLTGNPLFPYFNQYFRSPLALPLPYRDMRFL
                     PTDFWTAVSFPILFSIDWRVANDLPNLDIRVGLAYLAVIAALIVWALGRRAKEPLADP
                     KAVRIVFAFVAVSYFFWLKLFAIYRYILQLEMIAPLVIVLAVGLLPLARRTQLIAIGV
                     LFFAAMLFTRSAQLDHAPLGDPYITADLPTIADPARTMVVMTGDAPLGFIAPSLPREV
                     PVLRIDGWMFQPEDDSLLTRRTKARVYAHKGPLFLISDAYDMGRASAAVVDYGLAIDW
                     LKCRMFDTNLTGAYQWCPLVRRNP"
     gene            5695..6639
                     /locus_tag="VGC27_06075"
     CDS             5695..6639
                     /locus_tag="VGC27_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0282172.1"
                     /translation="MTLPDPRIAVLVPCCNEEATIAKVVADFRAGLPGAVVYVFDNNS
                     RDATAERAREAGAVVRFETRQGKGNVVRRMFADVEADIYVLVDGDDTYDASVAPALIG
                     KLIDEGLDIVSGWREATDASAYRRGHVLGNALLTGLTGLMFGVKLHDMLTGYRIMSRR
                     FVKSFPSAAVGFGIETELTVHAVRMKVPMAEARTRYKERPKGSASKLSTYRDGFRILL
                     TIAGMMRRERPLIFFTSIFAFFALFSFAIGIPVVIEYFQTGLVPRLPTAVLVTGMMML
                     AFLSLFTGLILDTVTRGRWEIKRLRYLAIPGPQDLKKP"
     gene            6636..7061
                     /locus_tag="VGC27_06080"
     CDS             6636..7061
                     /locus_tag="VGC27_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY0282173.1"
                     /translation="MTAERIFRRLSGSRFLRFAAVGGAGFLVNEAALVLAKELLGAGD
                     HAGWFIAFVPAVTFTWWGNRNLTFADKASNGPLGMLAEWARFVLTNSLGAAANFAVYA
                     ALIGWAPWPLNIPYIALAAGILAGLIFNFTLSKRLVFRN"
     gene            7118..7393
                     /locus_tag="VGC27_06085"
     CDS             7118..7393
                     /locus_tag="VGC27_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY0282174.1"
                     /translation="MRIEFDPVKSAKNERERGLPFAEATPLFDGPRLEWEDRRKDYGE
                     VRFNTLGEIEGRVFFVTFTRREEAVRIISFRKANTKETRLYRQHFSR"
     gene            7509..7748
                     /locus_tag="VGC27_06090"
     CDS             7509..7748
                     /locus_tag="VGC27_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0282175.1"
                     /translation="MVREGRVALVPPAKVDVRAIREKLGLSQSEFAAHFGFTAAAVRQ
                     WEQGRRQPHGPARVLLTIIAREPGAVSRALKSAAE"
     gene            complement(7772..>7979)
                     /locus_tag="VGC27_06095"
     CDS             complement(7772..>7979)
                     /locus_tag="VGC27_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282176.1"
                     /translation="GERDSISGAKLGAFSARPHAGATNRFAGKTLVDGIIHLPLVLPP
                     VAFGIRGPLGGWSRALPPSPSSRA"
BASE COUNT         1346 a         2598 c         2478 g         1557 t
ORIGIN      
        1 gcatgacgat gtcggtgagc aagagatcga agcggaagtc cgccagacat tcataggcgt
       61 cttcgccgtc gccgaaatcg gtgacgtcgt ggccggcgcg gatcagcgcc tgcgccagga
      121 acttgcgcaa ggactggtcg tcttccacca aaaggatatg cgccatgcac cgctccgccc
      181 gggggaacta aaccgtataa tccgggaacc gtaaatggcg gttaaagccg cgccccgccg
      241 gggagcttga tcggcggggc gggttgatgg tccactgccg cttgcgcttt ttgcgatccg
      301 gagctttccg ggcttttagc gggattgggc ggattttggc catggctggg acgggaaacg
      361 aggtggaaag cggcggttcc gatccgatcc tggcggagct ttaccgcgaa ccgttcagga
      421 tcgtgctgcc gcgggagcag acggcgccct tcgtcttcgc ctctccgcat tccgggcggc
      481 tttatcccgc ggatttcgtg gccaagagca accttgcgcc cttggcgctg cgccgctccg
      541 aggacgcctt cgccgacgaa ttatttgaga gcgcggtggg gcgtggcgcg ccgatgatcg
      601 ctgccaggtt cccgcgcgtc ttcctcgacg tcaaccgcgc gccatccgaa ctcgacagcg
      661 cgatgttcga cggcgccctt cacgtggagg tcggcgcgcc gagcacgcgg gttgcggcgg
      721 gtctcggggt aattccgcgc gtcgttcgcg acggcgccga gatttatcgc ggaagacttt
      781 tccccgccga cgccgagaaa cggctggcgc ggctttaccg gccttatcat gcggcgctgg
      841 ccggattgat cgaaaagacc cgcgcgcggt tcggcgtggc gatcctggtc gattgccatt
      901 ccatgccctc ggcatcgtcg atacccgaca tcgtgctcgg cgaccgctac ggcgtctcag
      961 cctctgcggc tctgatgcgc cacaccgaac gcgccttcga agcctgcgga ttttcgaccg
     1021 cgcgcaacac gccttatgcc ggcggctaca ccacgttgtt gtacggacgg cgcgaacagg
     1081 gcgtccacgc gctgcaaatc gaagtgaacc gcaccctgta tctcgacgag gcgcgcatcg
     1141 agcgccttgc ctgcttcgcc gaaacgcggg cacgcctgag cgaggcgctg ggcaaactcg
     1201 ttgccatgga cacgaatctg ttgatgccga atcccccgcg ccaatccctg gcggcggagt
     1261 aaaccggccg gcgttcggca ccatcttata gcgatgttgg cattctcatt ttcgcgcgtt
     1321 tgcgcgcata ttatcgcgcg tttacaagca ttttcgcgca taagaaaaag gccgctcccg
     1381 aaggagcggc caagttcagg gaggaaacgc ccaggaaggg ccgcggcgga gcggagagga
     1441 aatccgcaat gccgcgttgc aacaatatgc catcttatgg aggtgcaagc aagcaccaaa
     1501 tcaatgagac ttcaaaattt ttccgtacaa ataatccagt gtttccgcag taatattgtt
     1561 gcatgcatag attttgaccg gtaaatgcca tcttgttgtg cattgcacta tgggaccggt
     1621 accaccttta cagtgatgtc aactgcttag gatgtttcac agcattgtat tcgcgctgca
     1681 acaaaagatt cgcgcgcatc ggctacccag atcgcaggcc gcatcgcgcg attcgggcgg
     1741 caatagggag cccgcctatg gccgagacag cctggtcgcc aaattccttc gtcagacccg
     1801 aacacgccgc tccacggagc cgcagaaggc cacaagcgcc ctcccaggcc cattatgcgg
     1861 cggcgcacgg ccacagccac cgtacgatct tcatctccga cgtccatctg ggcacgcgcg
     1921 gctgcaaggc cgagctgctg gcggattttc tggcgcacaa cgactgcaac acgctgtatc
     1981 tcgtcggcga tattctcgac ggctggcagc tcaagcggcg ctggtactgg agcgaggcgc
     2041 agagccgcgt cgtgagcgag attttgcaca aggtcgatga aggcgtgcgg gtcgtctacg
     2101 tgcccggcaa tcacgacgaa tttctgcgcg cctattgcgg acgcaacatc gccggcatcg
     2161 aagtggtgcg cgagacgatc catgagaccg ccgccggcaa gcgcttttta atcctgcacg
     2221 gcgaccagtt cgacggggtc atcggctatg ccaaatggct ggcccatgtc ggcgactggg
     2281 cctattcggc cgcgctggcg ctcaacgacc ggctccacat gctgcgcagc agcctcgggc
     2341 tgccttactg gtcgctgtcg gcctatctca agaggaaagt caagaacgcc gtcgaatacg
     2401 tctcgcgcta tgaagaggtc gtggcgcgcg cggccgcgtc caaaggcgtc gatggtgtga
     2461 tttgcggtca catccatcag gcggaaatac gccaaatcgg ccccatcctc tacctcaacg
     2521 acggtgactg ggttgaaagc tgtaccgccc ttgtcgaaga tgcccgcgga catctggaga
     2581 ttctgcgctg ggcatcctgt tctcctgcga gcgactttgc ccgcgtttcg gcgggcgcca
     2641 aagagcttgt ctcgacaatc ctcgcgggcc gcgtttcgta gcgaaaatcg ctcggatggc
     2701 aggtggagcg gcgacagccg cgaccgcccg aagagcgtat cgggcaatcc ggtcgagggc
     2761 ttcgacaccc cagccggcga ttttcgccga aacccttcgg gacgcgccga ttttgcccgc
     2821 ccggttcgtc gccggtttcg gccgtactcc ccggtacgcc cttcaaccgg ctcctgccgg
     2881 atcgcggcaa aatcggcagc gtcgcggccg tgacgattat cgagacaggc tctgaggtga
     2941 aactgcgctt atacccgcgt aggatccgcc gccgcaggta tggcccgatg ccgaccggta
     3001 tcgggacggc cgaagccctc agcctcaagg tcctgatcgt caccgacgcc tgggcgcctc
     3061 aggtcaacgg cgtcgtgcgc acgctcgaaa tcctgggccg cgatttgcag gcgcttggcc
     3121 atgaagtgcg ttacgccacg ccggaaggcc gtttcaccct gcctttgcct acttattccg
     3181 agatcaggct ggcggttttt ccgcgcaccg tcttggaacg cgaaatccgc gaatacgccc
     3241 ccgacgcgat ccatatcgcc accgaaggca cgctgggcct gaccgcgcgc gccatctgcg
     3301 tgaagtaccg catccctttc accacttcgt tccacacccg ctttcccgat tatgtgcggg
     3361 cacgttttcc tttcatcggc gaagagacgg tctatcgctt cctgcgctgg ttccatcggc
     3421 ccgcgaccgc gatgatggtg gcgaccgctt cgctcaagcg cgaactcgag gaccgcggct
     3481 tccacaatgt gcgcatctgg tcgcgcgggg tggacaccga tcggttccgc ccgcttcccg
     3541 gcgcggcgct gccttatccc aagccgatct ggctttatgt cggccgggtg gccgtcgaga
     3601 agaatatcga ggctttcctc aagctggacc tgccgggcac caaggtggtg gtcggcaaag
     3661 gtccggcgct gacctcgctg gagcgtgcct acccggccgc cgagtttctc ggccagaaga
     3721 cgggcgaggc gctggtggag gtctacgcgg ccagcgacgt cttcgtcttt cccagccgga
     3781 ccgacacctt cggactggtg gttttggagg ccctggcctg cggtacgccc gtggcggcct
     3841 acccggtaca gggaccgaaa gacgtgatcg acgacgcgcg cgtggcggtc ctggacgacg
     3901 atctggcgaa ggcttgtcgc gaggcgctct caattccgcg cgacgccgcc aggtctttcg
     3961 cgctcagccg gtcatggcga tcctgcaccc agcagtttct ctccaacctg gcggtcgaga
     4021 tcgagccgta gggggcccct cccctcgacc cgaaaatgcg ctagatagag ccgggtgctt
     4081 caccgcttcg ccgggtccga tgtcttcagc caatcccact gcgcaatctc cgcgcctggc
     4141 gccgcgtgag ctggcctttc tcggtctctc catcctcgct tggacggcct atgtcgtttg
     4201 gctcggcaag gatacgtcct gggacttccg caattaccat tggtacgccc cttatgcgtt
     4261 cctgaacgga cgcatggaga tcgacgtcac cgtcgcccat caggccagct attacaatcc
     4321 gctcgccgac attcccttct attggctggc gatccacacc acgtcctggt tcgcgatcgc
     4381 cgtgctgggc ctgttccagg ccgccaacat cgttccgatc tatctgattg cgcgccacgg
     4441 cctgcgccac cagatgtccg aactcggatc ggcggcgatt gccttcttcg gcctgaccgg
     4501 cgggctgacg ctcagcctgt tcggcaccca ttactacgac aatgtgctga gcgtgttcac
     4561 gctcggcggc atcgccatcc tggtggtgaa gcgcgaggag ctggcgcgcg gctcgctcaa
     4621 aaaggcggcc ctgtggtgcg cgctcggcgg cctgatgacc ggcggcgcgg ccggcttgaa
     4681 gctgcccgag gcgccgttcg ccatgggctt cgccgcggcg ctgatcgcgg tgggcggcga
     4741 ccgcaagcat ctcttcaccc ggctcgccgc cggggcgctc ggcgggctgg ccggctttct
     4801 gctctgcgcc ggtttctgga tgtggcggat ggaagatctc accggcaatc cgctgttccc
     4861 ctatttcaac cagtatttcc gttcgccgct ggcgctgccg ttgccttatc gcgacatgcg
     4921 tttcctgccc accgatttct ggaccgccgt ttcgttcccc attctgttct cgatcgattg
     4981 gcgggtggcg aacgatctgc ccaatctcga catccgcgtc gggctggcct acctcgccgt
     5041 catcgcggcg ctgatcgtgt gggccttggg gcgccgcgcg aaggagccgc tggcggaccc
     5101 taaggcggtg cggatcgtct tcgcctttgt cgccgtgtcc tatttcttct ggctcaagct
     5161 tttcgcgatc taccgctaca tcctgcaatt ggagatgatc gcgccgctgg tcatcgtcct
     5221 ggccgtcgga ttgctgccgc tggcgcgccg cacccagttg atcgccatcg gcgtcttgtt
     5281 cttcgcggcg atgctgttca cccgcagcgc ccagctcgac catgcgccgc tcggcgaccc
     5341 ctacatcacc gccgatctgc ccacgattgc cgatcccgcg agaaccatgg tggtgatgac
     5401 gggcgacgcc ccgctcggct tcatcgcgcc gtccctgccg cgcgaagtgc cggtgctgcg
     5461 catcgacggc tggatgttcc agccggaaga cgattcgctt ctgacccgcc ggaccaaggc
     5521 gcgcgtctat gcccataaag gcccgctgtt cctgatttcc gacgcctacg acatgggccg
     5581 cgccagcgcc gccgtcgtcg attacggcct ggccatcgac tggctgaaat gccggatgtt
     5641 cgacaccaac ctcacgggcg cctatcaatg gtgcccgctc gtgaggcgca atccatgacc
     5701 ctgccggacc ctcgcattgc cgtcctcgtc ccctgctgca acgaggaagc gaccatcgcc
     5761 aaggtggtgg ccgatttccg cgccggcctt cccggcgcgg tggtctacgt cttcgacaac
     5821 aattcgcgcg acgcgacggc ggagcgcgcg cgcgaggccg gcgcggtggt gcgtttcgag
     5881 acgcgtcagg gcaagggcaa tgtcgtgcgc cgcatgttcg cggacgtcga ggccgacatc
     5941 tacgtgctgg tggacggcga cgacacctac gacgcctcgg tcgcgccggc tctgatcggc
     6001 aagctgatcg acgaaggact ggacatcgtt tcggggtggc gcgaagcgac ggacgcttcc
     6061 gcctatcgcc gcggccatgt gctgggcaac gcgctgctga ccggcctgac cggcctgatg
     6121 ttcggggtca agctgcacga catgctgacc ggctaccgca tcatgtcgcg ccgctttgtg
     6181 aaatcgtttc cttccgccgc cgtgggcttc ggcatcgaga ccgagctgac cgtgcacgcc
     6241 gtgcgcatga aggtgccgat ggccgaagcg cgaacgcgct acaaggagcg ccccaaaggt
     6301 tccgccagca agctcagcac ctaccgcgac ggattccgca tcctgctgac gattgccggc
     6361 atgatgcgcc gggagcggcc gttgatcttc ttcacctcca tcttcgcgtt tttcgcgctg
     6421 ttttcgttcg cgatcggcat tccggtggtg atcgaatatt tccagaccgg gctggtgccg
     6481 cgcctgccca ccgccgtgct ggtcaccggc atgatgatgc tggccttcct gtcgctgttc
     6541 accggcctca tcctcgacac cgttacgcgc gggcgctggg agatcaaacg cttgaggtat
     6601 ctggcgatcc ccgggccgca ggatttgaag aagccgtgac cgccgaacgg attttccgcc
     6661 gcttgtcggg ttcgcgcttc ctgcgctttg ccgccgtggg cggggcgggc ttcctcgtca
     6721 acgaggcggc gctggtgctg gccaaggagc ttttgggggc cggcgaccat gccggctggt
     6781 tcattgcctt cgttcccgcc gtgaccttca cctggtgggg caaccgcaat ctgaccttcg
     6841 ccgacaaggc cagcaacggc ccgctcggca tgctggccga atgggcgcgc ttcgtgctga
     6901 cgaacagctt gggcgcggcg gccaatttcg cggtctatgc cgcgctgatc gggtgggcgc
     6961 cctggccgct gaatatccct tatatcgcgc ttgccgccgg cattctggcc ggcctgattt
     7021 tcaatttcac gctgtcgaaa agactggtgt ttcggaacta ggatgcgtca ttgacatata
     7081 cgtcattgac atatagacgc tgagaagcta ttgtccgatg cggattgaat tcgatccggt
     7141 caagagcgcg aagaacgaac gcgagcgggg cctgccattt gcggaggcca caccgctatt
     7201 cgacggcccc cggctcgagt gggaagacag gcggaaagat tatggcgaag tccggttcaa
     7261 cacgcttgga gaaatcgaag gtcgcgtctt cttcgtcacg ttcacgaggc gagaggaagc
     7321 ggtccggatt atctcgttcc gaaaagcaaa taccaaggaa acgcggctct atcgtcagca
     7381 tttcagcaga tgaagcgcgc gcgacgaaga ttgattgggc gaaagtcgat gcaacgaccg
     7441 aagcggatat tgcccgccac gcgcgcgaag acggcacgca accgccatcc gatcgcgctt
     7501 ggcaaaagat ggttcgggaa ggccgcgtcg ccttggtgcc gcccgccaag gtagacgtgc
     7561 gcgccatccg cgaaaaactg ggtctgtcgc agtccgaatt cgccgcccat ttcggtttca
     7621 cggccgccgc cgtgcgtcaa tgggaacagg gccgccgcca gccgcacggc ccggcgcggg
     7681 tgctgctgac gatcattgcg cgcgaaccgg gcgctgtcag ccgtgcgctg aaatccgccg
     7741 ccgagtaatc accctgcctt ccgctccacc gtcacgctct tgacgatggc gaaggcggca
     7801 acgcccggct ccagccgccg agcgggccgc gtatcccgaa cgccaccggc ggcagcacca
     7861 ggggcagatg gatgattccg tccacaaggg ttttcccggc aaagcggttc gtagcgccag
     7921 cgtgcggacg ggcagaaaaa gcacctagct ttgcgccgga gatagaatcc cgctcaccc
//
LOCUS       DASWPU010000286         6562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_112814,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000286 DASWPU010000000
VERSION     DASWPU010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112814"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VGC27_06100"
     CDS             <1..686
                     /locus_tag="VGC27_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY0282177.1"
                     /translation="LTYNRYLSLARTNAIAPQTLDQARSTLQSAQATLISLQQNAQMQ
                     LAKLDGNPNLPPEQAPQYKQANAALDEAERQLSHTVVRAPFEGDVTEVDSLQPGTLVI
                     SALSALTTTSAVGLVSTTDTWVTANMKETDLTHVTVGDPVSVTIDTYPGRTWHGHVNA
                     ISRGSDSTFSALPSENASSNWVKVVQRIPVRVTLDVKADDPPLRAGMSVVVDIDTHHR
                     RWYRLFFGQ"
     gene            896..2473
                     /locus_tag="VGC27_06105"
     CDS             896..2473
                     /locus_tag="VGC27_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0282178.1"
                     /translation="MTAKPEIYGHEHYDSKFELMVVVLCSMSGVLMQALDTTIANVAL
                     PNMMGDLSASRDQITWVLTSYIVAAAVMTAPVGWVAARFGKKNVLIASMLGFTVASML
                     CGMAQNLDEMIVFRLLQGVFGAALAPLSQTVMLDLYPPEKRGQAMAIFGIGIMLGPIL
                     GPMLGGYLTDTYSWRWVFYVNVPVGVAASIGLWMFFKDTVHNTGLKFDWFGFAAMALG
                     VGALQLMLDRGTTKDWFSATEIVIECTMAGLGFYFFIVHMLTSKNTFIQRGIFSDRNF
                     MSAQIVMFVVGAVLLASTALLPPYLQTLSGHTVTEAGLLLGPRGLGTIVAMVLVGRMS
                     NHVDPRILMTIGTAAMTWSLWEMSRWTPSIDNYHLLFTTIVQGFGMGMVFSPLQLVAF
                     ATMPARLRPDGTALMNLVRNIGSAIGISVTTTLLSNSMQAIHSQLTAFATPFNRALGV
                     NAPSLLYNLQLPTGRAAFDGAIRIRAAIEAYANDFLFMFYFALITFPIIWLMHRPAYS
                     SSGGKPEERADVVEILE"
     gene            2473..3354
                     /locus_tag="VGC27_06110"
     CDS             2473..3354
                     /locus_tag="VGC27_06110"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0282179.1"
                     /translation="MHAAFIGLGVMGYPMAGHLARAGHQVTVFNRTKGRAERWTREYD
                     GRSAATPAEAAAEADMVLACVGRDADLRAVTLGGGGAFAAMKKGAVFVDHTTASATIA
                     RELGETAAARGFGFVDAPVSGGEQGAKNGQLTIMCGGSEADYAKAEPVIAAYAKQCRR
                     LGPVGSGQLTKMVNQICLAGMIEALSEGLNFARASGLDPQAVVDVISKGAAQSWQMDN
                     RHKTMLAGQYEHGFAVEWMRKDLGICFGEAARNGAALPVTRLVDTYYAEVEAMGGRRW
                     DTSSLFARLEAHRKGKR"
     gene            3354..3932
                     /locus_tag="VGC27_06115"
     CDS             3354..3932
                     /locus_tag="VGC27_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0282180.1"
                     /translation="MDIKRPYHHGDLRNALLDAARAILEEESLAALSLRAVARRARVS
                     HAAPYRHFPNHEALLVELAIEGFAELRAEIVAAAASPGGEADRIANLGAAYMRFVARR
                     PALTRLMYGPQVPNRSAFPALGLAADAIGEEIGKALHDSALGLAVWAAVHGLAMLILE
                     DVIDLGQRRSGLSVLPSRAEILLRSLFEVRRD"
     gene            4322..4792
                     /locus_tag="VGC27_06120"
     CDS             4322..4792
                     /locus_tag="VGC27_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282181.1"
                     /translation="MGEKHERPLGLWVLGLILITNGIVGGWIFLQLLTGRPYLLPLPL
                     SLVFQTGPIFDYAVTALMLGCCLVAGISLLLLSKRAVEFFAAAMVINVAYVIGTVLFW
                     FGQAVWLGLREGASFSLMDGIFLGILLVGSGAGVAIYLAVLNYIRSLSEYGILE"
     gene            complement(4805..5800)
                     /gene="hemB"
                     /locus_tag="VGC27_06125"
     CDS             complement(4805..5800)
                     /gene="hemB"
                     /locus_tag="VGC27_06125"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005179750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY0282182.1"
                     /translation="MAITPSFPALRMRRLRRHLWSRALVAETALSPADFIWPLFVIDG
                     QNKRVAVESMPGVERLSVDFAVRAAEEAAKLGIPVIALFPYTDPALKTADGREATNPD
                     NLICRAVRAIKKAVPEIGILCDVALDPYTTHGHDGLMVADYVVNDETVDVLVQQALVQ
                     VEAGCDIIAPSDMMDGRVAAIRAVLEKWGHKNTLIMAYAAKYASAFYGPFRDAVGSAK
                     ALIGDKRTYQMDPANGDEALREVALDIAEGADMVMVKPGMPYLDVVRRVKDEFGMPTF
                     VYQVSGEYSMLMAAIERGFLDRDRVILESLTGFKRAGANGILTYFARDAARLLSR"
     gene            5892..>6562
                     /locus_tag="VGC27_06130"
     CDS             5892..>6562
                     /locus_tag="VGC27_06130"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0282183.1"
                     /translation="MNDILFETRGAAGIITLNRPQALNALTHAMVVAMKAQLDAWAND
                     PAVAAVVVRGAGERGFCAGADIRAIAESGRAGTSYGHDFWRDEYILNAAIKHYPKPFV
                     ALISGIAMGGGLGLSVHGDYRIADETSLLAMPETGIGFFPDVGGSYFLSRCPGEIGMY
                     LALTGARLKAADALYAGIATHLVRADARAEIVDALARGENRAGLFSASEIPPPPDPSL
                     AERRIA"
BASE COUNT         1152 a         2068 c         2100 g         1242 t
ORIGIN      
        1 agctcaccta caatcgctac ctgtcgctgg cgcgcaccaa cgccatcgcg ccgcagacac
       61 tcgatcaggc ccgcagcacg ctgcaatcgg cgcaggcaac gctgatttcc ctgcaacaga
      121 acgcgcagat gcaactcgcc aagctggacg gcaatcctaa cctgccgccg gagcaggcgc
      181 cgcaatacaa acaagcgaac gccgcgctcg acgaagccga gcgccagctc agccacacgg
      241 tcgtgcgcgc gcccttcgag ggcgacgtca ccgaagtgga ttcgcttcag ccgggaacgc
      301 tggtgatttc ggccctgtcg gccttgacca cgacgagcgc cgtcggcttg gtgtccacca
      361 ccgacacctg ggtgacggcc aacatgaagg agaccgacct gacgcatgtg acggtcggcg
      421 atcccgtcag cgtcaccatc gatacctatc cgggccgtac ctggcacggg catgtcaatg
      481 cgatcagcag gggcagcgac tccaccttct cggccttgcc gtcggaaaac gccagcagca
      541 actgggtcaa agtggtccag cgtattcccg tgcgcgtgac gctcgacgtc aaggcggacg
      601 atccgccgtt gcgcgccggc atgagcgtgg tcgtcgatat cgatacgcat caccgccgtt
      661 ggtacaggct ctttttcggg cagtagggcg agtctcgata gatgcattct ccagcttcgc
      721 catggccgct cttggcccgt agcgcccgcg cacggacgcc atccagagaa acgaagaacg
      781 tgttcaccag ggtgaggcgc tgcatccgga tggccgggcc ggagcccggc catgacgaaa
      841 ataagcgggc gtggttacgt ccgacacaag aacgctccgg aacgcaaagc cgcgcatgac
      901 cgccaaaccg gaaatctacg ggcacgaaca ttacgacagc aagttcgagc tgatggtcgt
      961 cgtgctgtgc tcgatgtcgg gcgtgctgat gcaggcgctc gacaccacta tcgccaacgt
     1021 cgcgctgccc aacatgatgg gcgatctgtc ggcctcgcgc gaccagatca cctgggttct
     1081 gacctcctat atcgtggcgg cggcggtgat gacggcgccg gtgggctggg tcgccgcgcg
     1141 cttcggcaag aagaacgttc tcatcgcttc gatgctgggc ttcaccgtcg cctcgatgct
     1201 gtgcggcatg gcccagaacc tcgacgagat gatcgtcttc cgcctgctgc agggcgtgtt
     1261 cggcgccgcg ctggcgcccc tgtcgcagac ggtgatgctc gacctctacc cccccgagaa
     1321 acgcggccag gcgatggcga ttttcggcat cggcatcatg ctggggccga tcctgggccc
     1381 gatgctgggc ggctatctca ccgacaccta tagttggcgc tgggtcttct acgtgaacgt
     1441 gccggtcggc gtggcggcga gcatcggcct ctggatgttc ttcaaggaca cggtgcacaa
     1501 taccggcctc aaattcgact ggttcggctt cgccgccatg gcgctgggcg tgggcgcgct
     1561 gcaactgatg ctggaccgcg gcacgaccaa ggactggttc tcggcgaccg aaatcgtcat
     1621 cgaatgcacg atggccggcc tgggcttcta tttcttcatc gttcacatgc tgacctcgaa
     1681 gaacaccttc atccagcgcg gcatcttctc cgaccgcaac ttcatgtcgg cgcagatcgt
     1741 catgttcgtg gtcggcgccg tgctgctggc gagcaccgcc ctgctgccgc cctatctgca
     1801 gacgctttcc ggccacaccg tgaccgaggc cggcctgttg ctggggccgc gcggcctggg
     1861 caccatcgtg gcgatggtgc tcgtcggcag gatgtcgaac catgtcgatc cgcgcatcct
     1921 gatgaccatc ggcaccgcgg cgatgacctg gtcgctgtgg gagatgtcgc ggtggacgcc
     1981 gtccatcgac aattaccatc tgctgttcac gacgatcgtc cagggattcg gcatgggcat
     2041 ggtgttctcg ccgcttcagc tcgtcgcctt cgccacgatg ccggccaggc tgcggcccga
     2101 cggcaccgcg ctgatgaacc tggtgcgcaa catcggcagc gccatcggaa tttcggtcac
     2161 cacgaccttg ctgagcaaca gcatgcaggc gatccattcc cagctcaccg ctttcgcgac
     2221 gccgttcaac cgcgcgctcg gcgtcaacgc gccgtcgctg ctttacaacc tgcaactgcc
     2281 gaccggacgg gccgcgttcg acggcgccat ccggatccgc gccgccatcg aagcttacgc
     2341 caacgatttc ctgttcatgt tctacttcgc gctgataacc ttccccatca tctggctgat
     2401 gcaccgccct gcctattcca gcagcggcgg caagccggaa gaacgggcgg acgttgtgga
     2461 gattctggaa taatgcacgc ggctttcatc ggtcttggcg tcatgggcta tccgatggcc
     2521 ggccacttgg cccgcgccgg ccatcaggtg accgtcttca accgcaccaa gggcagggcg
     2581 gaacgctgga cgcgcgaata tgacggccgc tccgccgcca cgccggcgga agccgccgcg
     2641 gaggccgata tggtgctggc ctgcgtcggc cgcgatgccg atctgcgcgc ggtgacgctg
     2701 ggtgggggcg gcgccttcgc cgcgatgaaa aaaggcgccg tgttcgtcga ccatacgacg
     2761 gcctcggcca ccatcgcgcg cgagcttggc gagaccgccg cggcgcgcgg cttcggcttc
     2821 gtcgatgcgc cggtgtcggg cggggagcag ggcgccaaga acggtcagct caccatcatg
     2881 tgcggcggca gcgaagccga ctacgccaag gccgaaccgg tcatcgccgc ctacgccaag
     2941 caatgccgcc gcctggggcc ggtggggtcg ggccagctca ccaagatggt caaccagatc
     3001 tgtcttgccg ggatgatcga ggcgctgtcc gagggtctga atttcgcgcg cgcgtcgggc
     3061 ctcgatccgc aggcggtggt ggatgtcatc tccaaaggcg cggcgcaatc ctggcagatg
     3121 gacaaccgcc acaagaccat gctggcgggg caatacgaac acggtttcgc cgtcgaatgg
     3181 atgcgcaagg atcttggcat ctgcttcggc gaagcggcac gcaacggggc ggcgctgccg
     3241 gtgacaagac ttgtcgacac ttattacgcc gaagtcgaag cgatgggggg caggcgctgg
     3301 gacacctcca gcctgttcgc gcggcttgag gcgcatcgca aaggcaaacg ctgatggaca
     3361 tcaaacgccc ctatcatcac ggtgatctga gaaacgcgct gctcgacgcc gcgcgcgcca
     3421 ttctggaaga ggaatcgcta gcggcgctga gcctgcgggc cgtggcgcgc cgcgcacggg
     3481 tcagccacgc cgcgccctac cgccatttcc ccaatcacga ggcgctgctg gtcgaactgg
     3541 ccatcgaagg cttcgccgaa ctgcgcgcgg agatcgtcgc cgccgccgcg tcgccgggcg
     3601 gcgaagccga ccgcatcgcc aatctcggcg cggcctatat gcgcttcgtg gcgcgtcggc
     3661 cggcgctgac gcggctgatg tacggaccgc aggttccaaa ccgcagcgcc tttccggcgc
     3721 tgggactggc cgccgacgcc atcggcgagg agatcggcaa ggcgttgcac gattccgcac
     3781 tcggcttggc ggtgtgggcc gcggtgcacg ggctggccat gctgatcctg gaagacgtca
     3841 tcgacctggg ccagcggcgt tcgggcctct cggtgctgcc gtcgcgggcg gagatattgc
     3901 tgcgcagcct gttcgaggtg cgaagggatt aggttcacac gcagtccccc aattcgtcat
     3961 ggccgccttt aaggcggcca tccagcctgt tccgcgaagt gcttgcgcga aggcggatcg
     4021 gtgcgcgtcc gcgcgccgga aaactctttt tcgctgactg accgggtcat cttcaggtca
     4081 agctcgagga cataagctcg gccatggtga gtgcagtttg gtttttccag agtcggggga
     4141 taagtagggg aaagaatttg tttcttatcc tcgttttcga cagcctactt ccgctcaaca
     4201 gctgggcatt ttcaaaaaat gtacgcgcga acgcgggcca cgagggcctg ccgcccgtgt
     4261 gcaaacgttg atataagaga aatggtaatt cgagcagcaa ggttcaaggg ggcacttgaa
     4321 aatgggcgaa aagcacgagc ggcccttggg gctatgggtc cttggactta ttctcattac
     4381 caacggaatt gtcggcggtt ggattttttt gcagttgttg acggggcgac cgtatctact
     4441 tccgctccct ctaagccttg tgttccagac tgggccgatt tttgattatg cagttacggc
     4501 gctcatgctc ggttgctgtc tggtggcagg aattagtctt ctgttgttga gcaagcgagc
     4561 cgtggaattt tttgccgccg caatggtcat aaacgtcgcc tatgttatcg gaaccgtctt
     4621 gttttggttc gggcaagcag tctggctggg gttgagagag ggtgccagtt tctcgcttat
     4681 ggacggaatt ttccttggca ttctcttggt aggtagtggc gctggtgttg ccatctatct
     4741 tgcagtgttg aattacattc gaagtctgtc ggaatatggc atcttggaat agcagtgcgc
     4801 aaccctacct gctcaacagc ctcgccgcat cacgcgcgaa ataggtaagg atgccgttcg
     4861 cgccggcgcg tttgaaaccg gtcaggcttt ccaggatcac gcggtcgcgg tcgagaaagc
     4921 cgcgctcgat cgccgccatc agcatcgagt attcgccgga cacttggtac acgaaggtcg
     4981 gcattccgaa ttcgtccttc acccgccgca cgacgtcgag ataaggcatg cccggcttca
     5041 ccatcaccat gtcggcacct tcggcgatgt cgagcgccac ttcgcgcagc gcctcgtcgc
     5101 cgttcgccgg atccatctga taggtgcgct tgtcgccgat gagcgccttg gccgagccca
     5161 ccgcgtcgcg gaacgggccg tagaaggccg aggcgtattt cgcggcatag gccatgatca
     5221 gcgtgttctt gtggccccat ttttccagca cggcgcggat cgccgccacg cgcccgtcca
     5281 tcatgtccga cggcgcgatg atgtcgcagc cggcctccac ctggaccagc gcctgctgca
     5341 ccagaacgtc cacggtttcg tcgttgacga cgtagtcggc gaccatcaat ccgtcatggc
     5401 cgtgcgtggt gtagggatcg agcgccacgt cgcacaagat gccgatctcc ggcaccgctt
     5461 tcttgatcgc gcggacggcg cggcagatca gattgtccgg attggtggct tcgcgcccgt
     5521 cggccgtttt cagtgccgga tcggtgtagg gaaacagcgc gatcaccgga atgccgagct
     5581 tggccgcttc ctcggccgcc cggaccgcaa aatctaccga cagccgttcc acgccgggca
     5641 tggactcgac ggccacgcgc ttgttctggc cgtcgatcac gaacagcggc cagatgaaat
     5701 cggccggcga gagcgcggtt tcggccacca gagcgcgcga ccagaggtgg cggcgcagac
     5761 ggcgcatgcg aagggctggg aaggaaggag tgattgccat gggggagggc ttttgaattc
     5821 gcacccacta taggccatgc ttgcgcgcgg gcaacgcttc gattgccgcg cggcacgcca
     5881 ggacgacgga tatgaacgac attcttttcg aaacgcgcgg cgcggcgggt atcatcacgc
     5941 tgaaccggcc acaggcgctc aatgcgctca cccacgcgat ggtcgtggcg atgaaggcgc
     6001 agctcgacgc atgggcaaac gatccggccg tcgctgcagt ggtcgtgcgc ggggcgggcg
     6061 agcggggctt ctgcgccggc gccgatatcc gcgcgattgc cgagtccgga cgtgcgggca
     6121 cctcctacgg gcacgatttc tggcgcgacg aatacatcct gaacgccgcg atcaagcact
     6181 atcccaagcc ttttgtcgca ctgatctccg gtatcgcgat gggcggtggg ctgggtttgt
     6241 cggtgcatgg agactatcgc atcgccgacg agacatctct gctcgccatg ccggaaaccg
     6301 gcatcggctt tttccccgac gtcggcggca gctattttct gtcgcgctgt cccggcgaaa
     6361 tcggcatgta cctggcattg accggcgcgc ggctgaaggc ggcggatgcg ctttatgcgg
     6421 gaattgcgac tcatctggtg cgcgcggatg ccagagcgga aatcgtggac gccctggcgc
     6481 ggggcgaaaa ccgcgccggg ttgttctccg cgagcgaaat tcctccgccg cccgatccgt
     6541 cgctggcgga acggcgcatt gc
//
LOCUS       DASWPU010000287         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_39571,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000287 DASWPU010000000
VERSION     DASWPU010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39571"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VGC27_06135"
     CDS             <1..342
                     /locus_tag="VGC27_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282184.1"
                     /translation="VHGLGLMGIATRWTLDATSGRPKDSVRYCGAWFGVAKERREAIN
                     PTDPKISALMRLRRGMIGILAIVCSPPFPSAAQLIAEACRQAPSLAGMNLSGASSKKR
                     IERFLFRFDRS"
     gene            complement(556..1767)
                     /locus_tag="VGC27_06140"
     CDS             complement(556..1767)
                     /locus_tag="VGC27_06140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY0282185.1"
                     /translation="MGLYVFKLPDVGEGTTDAEIVAWRVKVGDIVKQEQELVDVMTDK
                     ATVEMTSPVAGRIVALHGELGEKATVGAPLVELEVEGEGNAKTMPVPASAENSVKEEP
                     APSAPVEAVASGQLLASPAVRERARALAIDLKSIPGSGPQGRISHTDLDAFIIGEASP
                     QSVAQGSIEEVKVIGLRRKIAEKMQESKRRIPHYTYVEEIDMTELEALRAQLNAARRA
                     DQPKLNVLPFLILALVRALPDFLQINALYDDEAGVVRRYREVHVGIATQTENGLLVPV
                     VRNAESRDLWDIAVEIARLAAAARENKATREDLSGSTITITSLGALGGLMATPVINRP
                     EVAIIGPNAIVERPAARDGRIEVRKMMNLSSSFDHRVIDGYDAAQFIRRIKALLEQPA
                     ALSVAVTAGPK"
     gene            complement(1770..>2056)
                     /locus_tag="VGC27_06145"
     CDS             complement(1770..>2056)
                     /locus_tag="VGC27_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0282186.1"
                     /translation="GEIIDLRTLVPLDLAAIVASVNKTGRCVIVHEATETGGYGGELT
                     ALVQKHCFYRLEAPIERVCGFDTPYPHGFEWEYFINPARVASAMRRTLEG"
BASE COUNT          356 a          617 c          637 g          446 t
ORIGIN      
        1 gtccacggcc ttgggttaat ggggatcgcc acgagatgga ctctcgacgc gacatcgggg
       61 cggccgaagg acagcgtgag gtactgcggg gcctggttcg gtgtggcaaa ggaaaggcgc
      121 gaggccatca acccgaccga ccctaagatt tctgccctga tgagattgcg acgtgggatg
      181 attggcattc tcgccatcgt ttgttcaccc ccctttcctt cagccgctca actcatcgcc
      241 gaggcgtgcc ggcaggcgcc atctctggca ggtatgaatc tctctggggc ttcatccaaa
      301 aaacgcattg agcgctttct ttttcgattt gaccgatctt gatcgattat aattgaatta
      361 gtcaaggcgg tgtgacaagg cgggtccgga acgtcagttg ctgttgagat ttggcatgca
      421 ggcctcccag acggggagcc cgtcgcctct ttgcctgccg cgacgccgtg ataggacaag
      481 tgatatctgg cttgaaggcc gctgacgaca ttttcgtatg ggcattttcg ccgggtttag
      541 cggtcagcaa accgtttatt tcggcccggc agttaccgcc acagacagcg ctgccggctg
      601 ttccaacagc gctttgatgc gccggatgaa ctgggctgcg tcgtagccgt cgatcacgcg
      661 gtggtcgaaa gacgacgaca ggttcatcat cttgcgcact tcgatccggc cgtcgcgtgc
      721 ggcggggcgc tcgacgatgg cgttgggtcc gatgatggcg acttcgggac gattgatcac
      781 cggcgtcgcc atcagcccac ccagcgcgcc caggctggtg atggtgatgg tggaaccgga
      841 caaatcttcc cttgtggcct tgttctcgcg ggcggcggcg gcaaggcggg cgatttccac
      901 agcaatgtcc cacagatcgc ggctttcggc attgcgcacc accggcacca gcaggccgtt
      961 ctcggtttgc gtggcgatgc cgacatggac ttcccggtag cggcgaacca cgcccgcttc
     1021 gtcgtcgtaa agcgcgttga tctgcaaaaa gtctggcagc gcgcgcacca gtgctaggat
     1081 caggaacggc agcacgttga gcttgggctg atccgcgcgc cgggccgcgt tcagttgcgc
     1141 gcgcagggct tccagctcgg tcatgtcgat ttcttcgaca taggtgtaat gcgggatacg
     1201 gcgtttagat tcctgcattt tttccgcaat cttgcggcgc agcccgatca ccttcacttc
     1261 ttctatgctg ccctgcgcaa cgctttgcgg cgaagcctcg ccgatgatga atgcgtctaa
     1321 atcggtgtgg ctgatacgtc cttgcggacc gctgccgggt atggatttaa ggtcgatggc
     1381 aagcgcccgc gcgcgttcgc gcactgcggg cgaggccaat agttgtccgc tcgcgacggc
     1441 ttcgaccggt gcggagggag ccggttcctc cttcaccgaa ttttctgccg aagccgggac
     1501 cggcatggtc ttagcgttgc cttcgccttc cacctccagt tcgaccagcg gcgcgccgac
     1561 cgtggctttc tcgcccagtt cgccgtgcag agcgacgatc cgtccggcga cgggcgaggt
     1621 catctccacc gtcgctttgt ccgtcatcac atcgaccagt tcctgttcct gcttgacgat
     1681 gtcgccgacc ttgacgcgcc aggcgacgat ctccgcgtca gtggtgcctt cgccgacgtc
     1741 agggagtttg aagacgtaaa ggcccatgtt cagccctcca gcgtgcggcg catggcggat
     1801 gccacgcgcg ccggattgat gaaatattcc cattcaaaac catgcggata gggcgtgtcg
     1861 aagccgcaga cgcgttcgat cggcgcctcg aggcgataga agcaatgctt ctgcaccagg
     1921 gcggtaagct cgccgccata gccgccggtt tcggtcgctt cgtgtacgat cacgcagcgc
     1981 ccggttttgt tcaccgaagc gacgattgcg gccagatcga gcggcaccaa ggtgcgcaga
     2041 tcgataatct cgccgt
//
LOCUS       DASWPU010000288         3722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_63950,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000288 DASWPU010000000
VERSION     DASWPU010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63950"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VGC27_06150"
     CDS             <1..871
                     /locus_tag="VGC27_06150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY0282187.1"
                     /translation="DWRAPPGRSHAEVAWMRLVAGIDPQWVPQILGEDQARHLFAMQY
                     FPPETYPVWKSLLAAGRADAGFAAQVGEAVARIHAATAGRDDIARAFANEAQFHALRI
                     DAYLLHTAAKHPDVAGIIRALAQSVSTARIALMHGDMSPKNILCGPGGPVFLDAETCC
                     TGDPAFDLAFCLNHLLLKAVWHPEHAAAFAASFVAMKDAYFAKADWEEHAGLENRTAG
                     LLAAFLLARIDGKSPVEYLTAEPDKAMVRALAKEFLNDRHKTLDTVLSRTVQKVSQAN
                     AGTMSVSSKSPPT"
     gene            complement(820..1233)
                     /locus_tag="VGC27_06155"
     CDS             complement(820..1233)
                     /locus_tag="VGC27_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0282188.1"
                     /translation="MIVIDSSAIVAILRAEPAADALTKRIAKEPPGARLMSVASYLET
                     GAVLAGRRKEKPLKAMDDLDGFLAEAGIDLADVDAEQAHTALRARIEYGRGFGASAGL
                     NFGDCFSYALAKSRSAPLLYVGGDFDETDIVPALA"
     gene            complement(1230..1481)
                     /locus_tag="VGC27_06160"
     CDS             complement(1230..1481)
                     /locus_tag="VGC27_06160"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY0282189.1"
                     /translation="MALSIKTKEADRLARTLSRLTGESMTEAVTKSLAERLMRVRKSR
                     EAGKDLATRIKAHAARLRGRYDLRPVTKAEWDEGWAEPK"
     gene            complement(1715..2194)
                     /locus_tag="VGC27_06165"
     CDS             complement(1715..2194)
                     /locus_tag="VGC27_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0282190.1"
                     /translation="MEHHKLDSIDMRILAELQADGRITNVELSRRAKITAPPCLRRMR
                     ALEKAGYIQGYHADVDAKLLGFEVTGYAFVGLSSQTDHDLRQFEEHVRSWPEVRECHM
                     LSGEVDFILKCVAKDLASFQNFITEKLTAEKNVASVKTALAIHATKVQPGVPLEIKG"
     gene            2455..3393
                     /gene="trxB"
                     /locus_tag="VGC27_06170"
     CDS             2455..3393
                     /gene="trxB"
                     /locus_tag="VGC27_06170"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY0282191.1"
                     /translation="MSGHSKVVILGSGPAGCSAAIYAARAMLEPVLIAGIQPGGQLTI
                     TTEIENYPGFAEAIQGPWLMQQMEEQAKRLGTRFVQDTIAAVDLAQRPFTLTGDSGER
                     YTADALIIATGAQANWLGLPSESKFQGFGVSACATCDGFFFRGKEVAVVGGGNTAAEE
                     ALFLTNFADHVTLIHRRDALRCDKTNQARLQANPKIEIAYNSEVVEVLGGEEPKGVGG
                     ALLRNRVTGETRTLAIDGLFVAIGHSPATALFKGQLKRDKSGYIKTAPDSTRTSVPGV
                     FAAGDVKDKVFRQAVTAAGMGCMAALEAERFLAGAS"
     gene            3393..>3722
                     /locus_tag="VGC27_06175"
     CDS             3393..>3722
                     /locus_tag="VGC27_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0282192.1"
                     /translation="MDWDKLRIFHAVASAGSFTHAGQMLALSQSAVSRQISALEDEIT
                     TPLFQRHARGLTLTPRGPWQRVRGGLRLGWAAGELPARALGCVPPLCRAARPRRRDRD
                     VAGGMNAP"
BASE COUNT          665 a         1209 c         1175 g          673 t
ORIGIN      
        1 ggactggcgc gccccgcccg gccgcagcca tgccgaggtc gcctggatgc gcctggtggc
       61 cggaatcgat ccccaatggg tgccgcagat cctgggcgaa gaccaagcgc gccatctctt
      121 cgccatgcag tactttccgc ccgaaaccta tccggtgtgg aaatcgctgc tggcggcggg
      181 ccgggccgat gccggattcg cggcccaggt cggcgaagcg gtcgcgcgta tccatgccgc
      241 cacggccggg cgcgacgaca tcgcccgcgc cttcgccaat gaggcacagt tccacgccct
      301 caggatcgac gcctatctgc tgcacaccgc ggccaaacat cccgatgtcg ccgggatcat
      361 ccgcgcgctg gcgcaaagcg tttcaaccgc gcgcatcgcg ctgatgcacg gcgacatgag
      421 tccgaagaac atcctctgcg gccccggcgg gccggttttc ctcgacgccg aaacctgctg
      481 caccggcgat ccggccttcg atctggcctt ctgcctcaat cacctgctgc tgaaagccgt
      541 ctggcacccc gaacacgcgg cggccttcgc cgcgtcgttc gttgccatga aggatgctta
      601 tttcgccaaa gcggattggg aggagcatgc cggcctggag aaccgcaccg ccggactgct
      661 ggcggcattc ctcttggcgc gcatcgacgg caaatcgccg gtcgaatatc tcacggccga
      721 acccgacaag gccatggtgc gggcgctggc gaaggaattt ttaaacgacc gccacaagac
      781 gctcgacacc gtgctgtcgc ggactgttca aaaagtttct caagccaacg ccggcacgat
      841 gtccgtttcg tcaaagtcgc cgccgacata aagcagcggc gccgaacggc tcttggcgag
      901 cgcataggaa aagcagtcgc cgaaattgag cccggcggaa gcgccgaagc cgcgcccata
      961 ttcgatgcgc gcccgcaagg ccgtgtgcgc ctgttcggcg tctacgtcgg ccagatcgat
     1021 accggcttcc gcgagaaagc cgtccagatc gtccatcgct ttgagcggct tttccttgcg
     1081 gcggccggcc agcacggcgc ccgtttcaag gtaagatgca accgacatga gacgcgcacc
     1141 gggcggttcc ttggcgatgc gttttgtcag cgcgtcggca gcaggctcgg cgcgcaggat
     1201 cgcgacgatg gcggaggaat cgatgacgat catttcggct cggcccagcc ttcgtcccat
     1261 tcggccttgg tcacggggcg cagatcgtat cggccgcgca agcgcgccgc gtgggccttg
     1321 atccgcgttg ccaaatcctt gccggcctcg cgggatttgc gtacccgcat cagacgctcc
     1381 gccagcgatt tggtgaccgc ctcggtcatg ctttcgccgg tcagccggct gagcgtgcgc
     1441 gccagccggt cggcttcctt ggtcttgatg ctgagggcca tgacacgcct ttctagaaac
     1501 aaattctaga tagcatggaa gcgtcggctt tgccagtata aaaatcggcg tgccctgtcc
     1561 tgacaagccc agcgcaagcg tcacggatgg ggttgtctct gcgtaaacgc gcccgcagag
     1621 acccgccaga ccacgaccta tcgtttcgtc gagcacgcgc acatgcactc tgtcgccgct
     1681 tgcggccggg acagcgttgg atttcgccaa tgtgctagcc cttgatctcc aacggcacgc
     1741 cgggctgaac cttggtggcg tggatggcca gcgccgtctt cacgctggcg acgttctttt
     1801 ccgcggtcag cttttcggtg atgaagttct ggaacgacgc cagatccttg gccacgcatt
     1861 tgaggatgaa atccacttcg ccggaaagca tgtggcactc gcgcacttcc ggccacgagc
     1921 gcacatgttc ctcgaattgc ctgaggtcgt gatcggtttg gctggacaat ccgacgaagg
     1981 cgtaaccggt cacttcgaag ccgagcagtt tggcatcgac atcggcatgg tagccctgga
     2041 tgtagccggc cttttcgagc gcccgcatcc gcctcagaca cggcggcgcg gtgatcttgg
     2101 cgcggcgcga aagttcgacg ttggtgatgc ggccgtcggc ttgaagttcg gcgagaatcc
     2161 gcatgtcgat ggaatcgagc ttgtgatgct ccacgggtag tccccggaaa tgaaagcgac
     2221 ggcatttaac ctagacgccc ccgaatgcgc aataatattt ccgcaagctc ggggtcgata
     2281 acacttggtc gagtcgtccg tttcgctttc cgggtgctgt caacagacat ctaagaccat
     2341 gtaatatata tgaaatctca tacgcttgac gtttgccgtc atggtcccga tatcaaacgt
     2401 cgcggcaccg gagcgggccg ctttcaccgt tcccccgaac aaaacgagac acaattgtcc
     2461 ggtcattcca aagtcgtgat tctggggagc ggccctgcgg gctgctccgc ggcgatctat
     2521 gcggcgcgcg ccatgctcga accggttctg atcgccggaa tccagccggg cggccagctc
     2581 accatcacca cggaaatcga gaattatccc ggcttcgccg aggcgatcca gggcccttgg
     2641 ctgatgcagc agatggaaga acaggccaag cgtctgggca cgcgcttcgt gcaggacacc
     2701 atcgccgccg tcgatctggc gcagcgcccg ttcacgctga ccggcgattc gggcgagcgc
     2761 tataccgccg acgcgctgat catcgccacc ggcgcgcagg cgaactggct gggcttgccg
     2821 tccgagtcga agttccaagg cttcggcgtt tcggcctgcg ccacctgcga cggattcttt
     2881 ttccgcggca aggaggtcgc cgtcgtcggc ggcggcaaca cggcggcgga agaagctctg
     2941 ttcctcacca acttcgccga ccatgtgacg ctgatccacc gccgcgacgc gctgcgctgc
     3001 gacaagacca accaggcgcg gctgcaagcc aaccccaaga tcgagatcgc ctataacagc
     3061 gaagtggtcg aagttctcgg cggcgaggag cccaagggcg tcggcggcgc cctgctgcgc
     3121 aaccgcgtga cgggggaaac ccgaacgctg gcgatcgacg ggctgttcgt cgccatcggc
     3181 cattcgccgg cgaccgccct gttcaagggc cagctcaaga gggacaaatc cggctatatt
     3241 aaaaccgcgc cggattcgac tcgcaccagt gtgccggggg tgttcgcggc gggggacgtg
     3301 aaagacaagg tcttccgcca ggccgtgacc gcggcgggca tgggctgcat ggcggcgctt
     3361 gaggccgaac ggtttctggc gggagcgtcg tgatggattg ggacaagctg cgcatcttcc
     3421 acgcggtggc atccgcggga agcttcaccc acgccggcca gatgctggcg ttgagccagt
     3481 cggcggtgag ccggcagatc agcgccctcg aagacgagat caccacgcct ctgttccagc
     3541 gccacgcccg cggcctgacg ctgacccctc gcggcccgtg gcagcgagtg cgaggagggc
     3601 ttcgtctcgg gtgggctgca ggcgagcttc ctgcacgtgc actgggctgc gttcccccgt
     3661 tgtgccgagc ggctcgcccg cgccgccgtg atcgagacgt cgcaggtggc atgaacgcgc
     3721 cg
//
LOCUS       DASWPU010000289         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_63951,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000289 DASWPU010000000
VERSION     DASWPU010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63951"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /gene="efp"
                     /locus_tag="VGC27_06180"
     CDS             <1..511
                     /gene="efp"
                     /locus_tag="VGC27_06180"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY0282193.1"
                     /translation="WMAVKSVKVKPGKGGAYNQVELKNVLTGTKLNQRFRSDEGVEEV
                     YLEKKDFQFLFATGDMLTFMDQETYDQIELSIEFVGEDQARFLQDGMKTLVQLYDGKP
                     IGIALPSNVTLAVTEADPVLRGGTASPSYKGAVLENGLKVQVPPFIEKGTRIVVSTED
                     GSYVRRAEQ"
     gene            511..1320
                     /locus_tag="VGC27_06185"
     CDS             511..1320
                     /locus_tag="VGC27_06185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0282194.1"
                     /translation="MPYLSPVLNVMVGAARKAGRLLIRDFGELENLQVSLKAPADFVS
                     TADERTERILIEELSRARPGYGFLAEETGTGGGPDKSHRWIIDPIDGTTNFLHGIPIF
                     GISIALEREGQLVSAVVYNPVIDEMYVAEKGHGAYLNDKRLRVAARKSMSEALFATGA
                     PFMGRPGRERCMAEIEAVLAVSPGVRRCGAASIDLAWVAAGRFDGYWERDLHPWDVAA
                     GILLVREAGGVVTDPDGGARMLESGHVVATNESLHPQILKLLKDAGKSGES"
BASE COUNT          286 a          474 c          497 g          262 t
ORIGIN      
        1 gtggatggcg gtcaaatcgg tgaaggtgaa gcccggcaag ggcggcgcct acaaccaggt
       61 cgagctcaag aacgttctca ccggcaccaa attgaaccag cgcttccgct ctgacgaagg
      121 cgtggaagaa gtctatctcg agaagaagga cttccagttc ctgttcgcca ccggcgacat
      181 gctgacgttc atggatcagg aaacctacga ccagatcgaa ctctccatcg aattcgtcgg
      241 cgaggaccag gcgcgttttc tgcaggacgg catgaagacg ctggtgcagc tctatgacgg
      301 caaacccatc ggcatcgcgc tgccgtcgaa cgtgacgctg gccgtgaccg aagccgaccc
      361 cgtgctgcgc ggcgggaccg cgtcgccgtc ctacaagggc gcggtgctgg agaacggctt
      421 gaaggtccag gtgccgccgt tcatcgaaaa gggaacccga atcgtggtct cgaccgaaga
      481 cggctcctac gtgcgcaggg cggaacagta atgccttacc tttccccggt tcttaatgtg
      541 atggtgggcg ccgcgcgcaa ggcgggccgt ttgctgatcc gcgatttcgg cgagttggag
      601 aatctgcaag tctcgctcaa ggcaccggcc gatttcgtct ccaccgccga cgaacgcacc
      661 gagcgcatcc tgatcgagga attgtcgcgg gcgcggccgg gctacggctt tctcgccgag
      721 gagacgggca ccggcggagg gccggacaag agtcatcgct ggatcatcga ccccatcgac
      781 ggcaccacga acttcctgca cggcatcccc atcttcggta tttccatcgc gctcgaacgc
      841 gaaggccaac tggtctccgc cgtggtctat aacccggtga tcgacgagat gtacgtggcc
      901 gagaagggcc atggcgccta tctcaacgac aagcgcttgc gggtcgccgc ccgcaaatcg
      961 atgtcggaag ccctgttcgc caccggtgct cctttcatgg gccggcccgg acgcgagcga
     1021 tgcatggcgg agatcgaggc cgtgctcgcc gtaagccccg gcgttcgccg ctgcggggcg
     1081 gcttcgatcg acctcgcttg ggtggcggcg ggccgcttcg acggctattg ggaacgcgac
     1141 ctccaccctt gggacgtggc ggccggcatc ctgctggtgc gcgaagcggg aggcgtcgtc
     1201 accgacccgg atggaggagc gcggatgctg gaatcggggc acgtggtcgc caccaacgag
     1261 agcctccacc cgcaaatcct gaagcttctg aaagacgccg gaaagtccgg cgagagttga
     1321 gcgcggcaag cggtgcgtct atggtgcacg actgattaaa gcaggcccaa agcgatgcgg
     1381 tattttttcg ccctgacggt atttctcgcg gctgccgcgt cgccgccggc cacggcgcag
     1441 acggtccccg gcgagaacgt catcgtgaac ccctctgccg cgggtacccg ggtgctgcgc
     1501 tatccgggcg acaaatatg
//
LOCUS       DASWPU010000290         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_39600,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000290 DASWPU010000000
VERSION     DASWPU010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39600"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VGC27_06190"
     CDS             complement(<1..672)
                     /locus_tag="VGC27_06190"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEY0282195.1"
                     /translation="MAQNVPTPKPQTVDDVIANILRDSPETASPAARKGQAVAAQAPA
                     AEPIVMGARIGEHPDRTRFVVELSDPVDIRTFTLSSPNRVVIDLPAVQWRLEGPPRPS
                     GHGAVKSYRYGLFRPGNSRFVIDLEKPVIVSDILVLPPERGFGYRVVIDLFPTTQAKF
                     DRTAGWPADLKAREYAAEQSATPPQQPAAPRTARKIIVIDPGHGGLDSGTVGVGGAME
                     KDIVLA"
     gene            1186..>1718
                     /locus_tag="VGC27_06195"
     CDS             1186..>1718
                     /locus_tag="VGC27_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY0282196.1"
                     /translation="MPKRMLIDASHPEETRVVVLDGQKVEEFDFEAASKRPLKGNIYL
                     AKVTRVEPSLQAAFVEYGGNRQGFLAFSEIHPDYYQIPVADRIALLAEQEADARRRQE
                     DDFESFDIRSGRKADAPAQEPETNGDGPDEAQAPAEGENGFSEPNGWDESRDSGAPSE
                     DGGPAQDEQAAPDEFPEG"
BASE COUNT          352 a          530 c          533 g          303 t
ORIGIN      
        1 ggcaagaacg atatcctttt ccatcgcgcc gcccacgccg acggtaccgg aatcgagacc
       61 gccatgacct ggatcgatga cgatgatttt cctggcagta cgaggcgcag caggctgttg
      121 cggcggagtg gcactctgtt cggcggcgta ttctctggcc ttgaggtccg ccggccagcc
      181 tgcggtgcgg tcgaacttcg cctgcgtggt cggaaacagg tcgatgacga cgcggtagcc
      241 aaaaccccgc tccggcggca gaacaagaat gtcgctcacg atgacgggct tctccaaatc
      301 gatcacgaaa cgggaattac ccggccggaa cagcccatag cggtagcttt tcaccgctcc
      361 atggccggaa ggccgcggcg gtccctccag gcgccactgc acggccggaa ggtctatgac
      421 cacacggttg ggactggaca aggtaaaagt gcggatatcg acaggatcag atagttccac
      481 cacgaatcgc gtgcggtcgg ggtgctcgcc aatgcgcgcg cccatgacaa tgggctcggc
      541 ggcgggagct tgtgcagcaa ccgcctgtcc cttccgggca gccggagaag ccgtttcagg
      601 cgaatcccgg aggatattag cgataacgtc gtcaacggtc tggggcttgg gcgtaggcac
      661 attctgggcc acagccgacc ctagcaacag gcaagccact atgcctaaag cgatatttcg
      721 cacttttcgc atcgcgtccg gaacagccct agacctcaca accgaaggtc gggcataaac
      781 catttacgtt tcgttagcat ctggggattt tgaatgattg ccggggggtc ctcttgccgt
      841 agtgcggcgg gacacctaca actgcttaaa gctgcgtcgc aacaggcagg ccttggcctg
      901 tcaggtgggt ccggacgcag gccaagaatg tccgaggacg gtccttgggt tttggccgcc
      961 aggttttcca gccgcggcag cagctttcct caccaagcgg cttcgcgccg cttggcctcg
     1021 aacgtgatga gatgaacgac ttagcgctcc ccattttgta ccagcttgcg gcaccgccgc
     1081 tggcggcaac acggcggcgc ctgtccccaa caaggcggcc catttcgagc ccggatcgcg
     1141 cgcagccgcg cccggcaggc tcctgcgcgc cgcccggaga tttctatgcc caagcgtatg
     1201 ttaatcgatg caagccaccc ggaagagacc cgagttgttg ttctcgacgg ccagaaggtc
     1261 gaggaatttg atttcgaggc cgcttccaag cggccgctca aaggcaatat ctatctcgcc
     1321 aaggtaaccc gcgtggagcc gtcgttgcag gcggctttcg tcgaatatgg cgggaaccgg
     1381 caaggcttcc tcgccttttc ggaaatccat cccgactact accaaattcc ggtcgccgac
     1441 cgcatcgcgc tcttggccga gcaagaggcg gatgcgcgac ggcgccaaga ggacgatttc
     1501 gaatccttcg atatccgctc cggacggaag gccgatgctc ccgcgcaaga acccgaaaca
     1561 aacggggacg gcccggacga agcgcaagcg ccggccgaag gggagaacgg cttttccgaa
     1621 cccaacggtt gggacgagag ccgagatagt ggcgctcctt ccgaagacgg cggcccggca
     1681 caggacgaac aagcggcgcc ggatgagttc cccgaagg
//
LOCUS       DASWPU010000291         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_87901,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000291 DASWPU010000000
VERSION     DASWPU010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87901"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VGC27_06200"
     CDS             <1..445
                     /locus_tag="VGC27_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEY0282197.1"
                     /translation="ITLLYPNQKSYELAYFWALAGTVQGILWPGILFDFPEPRFIEFS
                     IFHGGIIASVIFLTLGMKMRPYPRSVPRVWAWSLFYMAAAGAVDWALGVNYGFLRSKP
                     IHPSLFDLMPDWPYYLPLLVVLGVLSTLICYAPFFLADRMRALRK"
     gene            complement(739..1425)
                     /locus_tag="VGC27_06205"
     CDS             complement(739..1425)
                     /locus_tag="VGC27_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0282198.1"
                     /translation="MTTILVTGANRGIGLEFVRQYAAEGADIIACCRKPSEAKELQAL
                     AKTGKVRAMALDVGDASSAAALAQVLRGEAIDILINNAGIYGPDERSARESYYDGFAR
                     TFRINSIGPLLVSQALRDNLLAGRDKKLAVLTSKMGSISDSGSGALAYRASKAAVNMI
                     MHVLAREWAADGILVAILHPGWVKTDMGGPGALITPQESVAGLRARIAELSPQTSGRY
                     LDYRGKEIAW"
     gene            1479..2195
                     /locus_tag="VGC27_06210"
     CDS             1479..2195
                     /locus_tag="VGC27_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY0282199.1"
                     /translation="MSIWGKISGAAAGMLVGGPVGAAVGVLAGHFLIDRAAPEGESDP
                     GVAFTIAMIALAAKMAKADGVVTDEEVEAFDRVFRVPPEEQAAVRRFFDLARQDTAGY
                     ESYAGRIAHLYAGKPAVLEDILDGLFEIAKADGTMHPGEDAFLERVADIFGFAPGEYR
                     RIRASHFTPDAADPYAVLGVAYEASDDEIKRTYYRLVRENHPDSLIARGVPKEFIRLA
                     TDKLAAINGAYEKIQAERAS"
     gene            2357..2698
                     /locus_tag="VGC27_06215"
     CDS             2357..2698
                     /locus_tag="VGC27_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282200.1"
                     /translation="MSKLDIGVGEEFPVDEEEKREEAPGGDANPRHCGRGHRFGGRHG
                     ERREALRAWRNQMRSEWKERKRAMHERFHREFPHGEFREHTHHRMVGTLAVAGLALIG
                     LAALLGRHHSE"
     gene            2710..>2943
                     /locus_tag="VGC27_06220"
     CDS             2710..>2943
                     /locus_tag="VGC27_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY0282201.1"
                     /translation="MHGHHRRFRGMTVEAMRAAAAAMEAAGETGGENGGRATTRVQLE
                     MPPQAMERLQKLKDRTEAASYAEVIRNALRLFEA"
BASE COUNT          532 a          919 c          927 g          565 t
ORIGIN      
        1 gatcacgctg ctttatccca accagaagag ctacgaactg gcctatttct gggccttggc
       61 gggcacggtg cagggcatct tgtggccggg cattctgttc gattttcccg aaccccgctt
      121 catcgagttc tcgatcttcc acggcggcat catcgcctcg gtgatattcc tgaccctggg
      181 catgaagatg cgcccctacc cccggtcggt gccgcgggtc tgggcttggt cgcttttcta
      241 tatggcggcc gcgggggcgg tggattgggc gctcggcgtc aattacgggt tcctgcgctc
      301 caagccaatc cacccgagcc tgttcgatct gatgccggac tggccttatt acctgccgct
      361 gctggtggtt ctgggtgtgc tttcgacgct gatctgttat gcgccgttct tcctggccga
      421 ccggatgaga gcgctgcgga aatagcaagg ccggaagcat cgcccccggc ctttatggaa
      481 actctcgaat gcaacgctca gaagacgccg acaaccaccg ccccaagaaa caagaacacc
      541 agagcgacgg tcataagctg tagctctcgc gtcaaggtct cctccctggt ttgaagacca
      601 tgagagtctc ggttgaggtt cacgtaaggt caagcctgcg gcagccggga tttaattttc
      661 atcatgtttt acaatgggtt aatcttaagt ttcatgccgg ggccatgctt ctggcgcgca
      721 cgtttatttc acggcagctc accacgcaat ttcctttccc cgatagtcga ggtagcggcc
      781 ggaggtctgc gggctgagtt ccgcaatgcg cgcgcgcagg ccagccacgc tttcctgcgg
      841 cgtgatcagc gcccctggcc cgcccatgtc ggttttgacc cagcccggat ggaggatggc
      901 gaccaggatg ccgtcggcgg cccactcgcg cgccaagacg tgcatgatca tgttcaccgc
      961 cgcctttgag gcgcgatagg cgagggcgcc gctgccgctg tcggaaatcg agcccatttt
     1021 gctggtcagg acggcgagct tcttgtcgcg gccggccaac aaattgtcgc gcagcgcctg
     1081 gctgacgagc agcgggccaa tgctgttgat gcggaaggtg cgggcaaagc cgtcataata
     1141 gctttcccgg gcgcttcgct cgtcggggcc gtagataccg gcattgttga tcaggatgtc
     1201 gatggcttcg cctctcaaaa cctgcgccag cgcggccgcc gacgaggcgt cgccgacgtc
     1261 cagcgccatc gcccgcacct tgccggtttt ggcgagcgcc tggagttcct tcgcttcgga
     1321 cggcttgcgg cagcaggcaa tgatgtcggc gccttcggcg gcatattgcc ggacgaattc
     1381 cagcccgatg ccgcgattgg cgcccgtcac cagaatcgtt gtcatctttc ttctccgtga
     1441 gttgtatttc cgccgagttt gccgtttagc ttcccatcat gtccatctgg ggaaaaatca
     1501 gcggcgcggc ggccggtatg ttggttggcg gacccgtagg cgcggcggtg ggcgtgctgg
     1561 cgggtcattt cctgatcgac cgcgcggcac cggagggcga aagcgatccc ggggtcgcct
     1621 tcaccatcgc catgatcgcg ctcgccgcca agatggccaa ggccgacggc gtggtgaccg
     1681 acgaggaagt ggaagccttc gaccgcgtct tccgcgtgcc gcctgaggaa caagcggctg
     1741 ttcgccgctt tttcgatctg gcgcggcagg acacggccgg ctacgagagc tatgcgggcc
     1801 ggatcgcgca cctctacgcc ggcaaaccgg cggtgctgga agacatactc gacggcctgt
     1861 tcgagatcgc caaggcggac ggcacgatgc atcccggcga ggatgcgttc ctggagcggg
     1921 tcgccgatat tttcggtttt gcgccgggcg aataccgtcg catccgcgcc tcgcatttca
     1981 cccccgacgc ggccgatcct tacgcggttc tcggcgtggc ctacgaagcg agcgacgacg
     2041 agatcaagcg cacctactac cgtctggtgc gggagaacca tcccgacagt ctgatcgccc
     2101 gcggcgtgcc caaggagttc atcaggctcg ccaccgacaa gctcgccgcc atcaacgggg
     2161 cctacgagaa gatccaggcc gagcgcgctt cctgaaattt ccaggcattc cggcaaaggc
     2221 gcccggcagc ggcgctggcg gccgcgcgct ttttattacc ccaaaaggtg gcctcaaaag
     2281 acggcttgac atttgatgat attatgatga tatatccctt atcaggccgc caatgcggcc
     2341 cccaaggaga atatttatga gcaaactaga cataggcgtc ggcgaggaat ttcccgtcga
     2401 tgaagaagaa aagcgtgaag aggcgcccgg cggagatgcc aacccgcgcc actgcggccg
     2461 cggccatcgt ttcggcggac ggcacggcga gcgccgcgag gccctgcggg cttggcgcaa
     2521 ccagatgcgc agcgaatgga aagagcgcaa acgcgcgatg cacgaacggt ttcaccgcga
     2581 attcccgcat ggcgaatttc gggaacatac gcaccatcgt atggtcggca cgcttgccgt
     2641 cgcggggctg gcgctgatcg gtttggccgc gttgctcggc cgtcaccact cggaatgagg
     2701 atcgcgacca tgcacggaca tcatagacgg tttcgcggca tgacggtgga agccatgcgc
     2761 gccgccgccg ccgccatgga ggccgccggc gagaccggcg gcgaaaatgg cgggcgcgcc
     2821 accacgcggg tgcagctcga aatgccgccc caggcgatgg aacgcctgca aaaactgaag
     2881 gaccgcaccg aagcggcgtc ctacgccgaa gtcatccgca acgccttgcg cctgttcgaa
     2941 gcg
//
LOCUS       DASWPU010000292         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_2333, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000292 DASWPU010000000
VERSION     DASWPU010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2333"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VGC27_06225"
     CDS             complement(<1..727)
                     /locus_tag="VGC27_06225"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0282202.1"
                     /translation="MNNDSDPNGDALSIVSVTNGTLGTVITNGVSVTYTPAYDGNGRL
                     STVAYPSGLTVRYVYTALGYVSQIKDNGSGLAYWTANGRDAELHLTSQSFGNGVVQTN
                     TFNANTGLPAAFRAGSSDSVAAFDYAWDQVGNLTYRSDNRQGTFEYACYDALNRLTQY
                     AAGNGVTACTSPQNQKTVGYDALGNIASKTGVGTYRYAAAGQARPHAVSSITGTVNGA
                     VDPGYSYDANGNLTAGAGRTVSYT"
     gene            complement(793..1116)
                     /locus_tag="VGC27_06230"
     CDS             complement(793..1116)
                     /locus_tag="VGC27_06230"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbC/YhhH family protein"
                     /protein_id="HEY0282203.1"
                     /translation="MKNLQRIGVIILLVFALGPRALGQSEHNVRPRAGIVPDQVTAIR
                     IAEAVLIPIYGQEQIKAERPFTAKLNTNTWIVMGYIPPGVDGGVAEVWIDKRDGKILR
                     VTHGK"
     gene            complement(1450..>1801)
                     /locus_tag="VGC27_06235"
     CDS             complement(1450..>1801)
                     /locus_tag="VGC27_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEY0282204.1"
                     /translation="ARYYSSTIGRFLQTDPVGYTDDINLYAYVGNDPTNSTDPSGLTP
                     QTANSNAPEVSSACSRIGVTSCSGSYAGNTNDAKDGPPGQSQQSPVKAVGSSCTRNPV
                     QKQRRGGYCGRKNL"
BASE COUNT          355 a          506 c          513 g          427 t
ORIGIN      
        1 aggtgtagct caccgtccgc cccgccccag ccgtcaggtt gccgttggcg tcgtaactat
       61 agcccggatc aaccgccccg ttcaccgtgc cggtgatgga cgacaccgcg tgcggccgcg
      121 cctggccggc ggccgcatag cggtaggtgc ccacccccgt cttcgaggcg atgttgccca
      181 gcgcgtcgta gccgaccgtc ttctggttct gcggcgaggt gcaggccgtc accccattgc
      241 ccgccgcata ctgcgtcagc cggttcaggg cgtcgtagca ggcatactcg aaggtgcctt
      301 gccggttgtc tgagcggtag gtgagattgc cgacctggtc ccaggcgtag tcaaaggcgg
      361 caaccgaatc gctcgaaccc gcacggaacg cggctggaag ccccgtgttg gcgttgaagg
      421 tgttggtttg gaccaccccg ttgccgaagc tttgtgaggt caggtgcagc tccgcatccc
      481 gaccattggc cgtccaatag gccaagccag agccgttgtc cttgatctgg gagacgtagc
      541 cgagcgcagt atagacgtac cggacagtca ggccagaggg atacgccacg gtggatagcc
      601 gtccgttgcc gtcataggcg ggcgtgtagg tgactgacac gccgttcgtg ataaccgtgc
      661 ccagtgttcc gttcgtcaca gacacaatgc tgagagcatc gccgttcggg tcgctgtcgt
      721 tgttcatcgg attaagcgac ttggacggct taacgcagct gccgtgggac acgtgcgcag
      781 tcactgtatt ctctactttc cgtgcgttac gcgcaaaatt tttccatcgc gcttgtcaat
      841 ccacacttcg gcaacgccgc cgtcgactcc aggcgggata tatcccatga ctatccaagt
      901 attggtattc aatttcgccg taaaaggacg ttcggcctta atctgttcct gtccataaat
      961 cggaatcagg acagcttcag caatacggat tgctgtcact tgatcaggca caatacccgc
     1021 ccgcggcctc acgttatgct cgctttgacc taaagcccgc ggccctagtg caaacacgag
     1081 caaaataatt actcctatcc gctgaaggtt tttcatggcg tggtgtccct gaaagtggtg
     1141 atggtgccgg tcgctggatc aaactttcta atcgctccaa atggagttcc gagaaataag
     1201 ggaactcccc gagctcttgc gttgtccata tctgcagtcg agaaatactc attgccatag
     1261 ttcggggaaa atccaccgtg tgtgtgatat gtctccactc ggatggcccc ccgaggcaac
     1321 gtacttatgg caggaggcga tgacatatac tcggaaccga ttgcgggcgt ttgaatgtag
     1381 cagcaatcat catcggaata ctgatagatg ttgccattaa tttctcggtt catcgccttt
     1441 gaataaggat tatagatttt ttcggccgca atacccgccg cgtctttgtt tctgtaccgg
     1501 gttcctggtg caggaggaac ccaccgcctt taccgggctt tgttggcttt gacccggagg
     1561 accatctttc gcatcatttg tgttacctgc ataagaaccg ctgcaacttg tgaccccaat
     1621 tctcgaacaa gcactactaa cctccggtgc atttgagttc gccgtttgtg gtgtgaggcc
     1681 actcggatca gttgagtttg tcggatcatt tccgacatag gcgtagaggt tgatgtcgtc
     1741 cgtgtagccg acggggtcgg tctgcaggaa gcggccgatg gtggaggagt agtagcgcgc
     1801 g
//
LOCUS       DASWPU010000293         9825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_21148,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000293 DASWPU010000000
VERSION     DASWPU010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21148"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VGC27_06240"
     CDS             <1..1152
                     /locus_tag="VGC27_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY0282205.1"
                     /translation="RNLNIWYGFGVILTFFLGLQIVSGVVLAMHYVASADLAFNSVEK
                     IMRDVNYGWLIRYLHANGASMFFLAVYIHMFRGLYYGSYKEPREVLWILGMLIYVLMM
                     ATAFFGYVLTWGQMSFWGATVITSLFSAIPYVGDAITAWLWGGFAVGDATLNRFFSLH
                     YLLPFAIAAVVGLHIWALHVPGNNNPLGIDVQGPQDTVPFHPYYTVKDAFYLSLFLIL
                     FAGFVFFAPDFFGNPDNYTPANPLVTPPDIVPEWYLLPFYAMLRAVPQKLIGVLVLVG
                     ALVIPFIVPWLDTSKVRSCRFRPLMKPCFWLFVLDCVLLGYLGSQGADAAFDLGGMQL
                     PLVWLARLGTAYYFAYFLVLMPLVGLIEKPKPLPESIARSVLGAKGERT"
     gene            1149..1943
                     /locus_tag="VGC27_06245"
     CDS             1149..1943
                     /locus_tag="VGC27_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY0282206.1"
                     /translation="MKIALPLFVLAFGLSFGSAAADNSAALPPRHMQWSFAGPFGTYD
                     RGALQRGFKVYKEVCAACHGLDLVAFHDLANPGGPGFTEAQAKAIAAGYRIPAEPNDK
                     GEIFDANGNRLTRPGILADRFPPPFPNEEAARASNNGSLPPDLSLIAKAHVGGPDYLY
                     SLLVGFGHKPPKDFKVLEGKYYNPYFAGRNIAMPPPLMAGSVSYPDGTKTSVAQEAHD
                     VVTFLAWAAEPKLEERHRIGFQVMAFLVLLAGLLFLTYRKVWKGEH"
     gene            2190..3089
                     /locus_tag="VGC27_06250"
     CDS             2190..3089
                     /locus_tag="VGC27_06250"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY0282207.1"
                     /translation="MSRTVLGIIGGSGVYDIGGLENPRWERVEGPWGEPSDELLLGTY
                     AGVDLVFLPRHGRGHVRTPSTVNYRANIDALKRAGVTDVLSVSACGSLKEELAPGTFV
                     FVDQFIDRTFAREKTFFGPGFVAHVSMAHPVCPRLTGALASAAREKGIRHARGGTYIC
                     IEGPQFSTLAESNLYRSWGCSVVGMTAMPEAKLAREAELPYALVAMVTDFDCWHPDRD
                     HVTVDAVVKVLLANAEQARKLICQAATKLGPERSPSPLGIENALDTAMITAPEKRDPQ
                     LIAKLDAVAGRVLSKVEADPAGQ"
     gene            3176..3703
                     /locus_tag="VGC27_06255"
     CDS             3176..3703
                     /locus_tag="VGC27_06255"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY0282208.1"
                     /translation="MDFASVIRTIPDYPKKGILFRDVTTLFGNPRAFRAAVDALVQPY
                     AGVRIDKVAGIEARGFILGGAVAHQLSTGFVPVRKKGKLPYNVIGEEYDLEYGKDRVE
                     IHVDAVKKGEYVVLVDDLIATGGTAFASIKLLERAGAKVVGCAFVIDLPELGGADKIR
                     ALGKEVTTLVSFEGH"
     gene            3963..4574
                     /locus_tag="VGC27_06260"
     CDS             3963..4574
                     /locus_tag="VGC27_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0282209.1"
                     /translation="MKLYMSPASPFARKARIIIRELDLTRLVEEIPTDPATSGELRKT
                     NPLGKIPVLVLDDGSTLIDSPVICEYLNEMGGGKFFPGMSIWRSASGRWRALTLQALG
                     DGLAEAAVARLYERRRPEELQNKEAIAKYLAAIGRSLDVLERARFAKPPTIGEIAVAC
                     AIGYLDFREVMPGWRDMRPQLRGWYEDFVKYPAMQETWPANLT"
     gene            4553..5650
                     /gene="mtnA"
                     /locus_tag="VGC27_06265"
     CDS             4553..5650
                     /gene="mtnA"
                     /locus_tag="VGC27_06265"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEY0282210.1"
                     /translation="MAGEPDVKIAGRHYRTIWPRADEAVEVIDQTRLPHAFATAVLRT
                     AEDCAQAIRSMVVRGAPLIGATAAYGVAFAMRAEPSSKALVAALDLLQATRPTAVNLR
                     WALKRMAGVLQSRSHGDRFVLAWNEAAAICDEDVEVCRSIGEHGLKLLREAAAKKNGA
                     PLDILTHCNAGWLACVDWGTALAPIYMAHDAGMDVHVWVDETRPRNQGASLTAYELGA
                     HGVPHTIITDNAGGHYMQTGRVDLCFVGTDRVAMNGDVANKIGTYLKALAAKDNNVPF
                     YVALPSSSIDWTLASGTEIPIEERSPDEVLKMTGRLPDGTIATVDIAAPGSPAANPGF
                     DVTPARLVTGFVTERGICAASEAGLRGLFPA"
     gene            complement(5753..6427)
                     /locus_tag="VGC27_06270"
     CDS             complement(5753..6427)
                     /locus_tag="VGC27_06270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0282211.1"
                     /translation="MAAGKMIFLTMSDGKAIGVYRVQPHGVRRGGLVLIQEVFGVTGH
                     IKAVSDSYAAEGYEVLAPSLFDREAPGFISGYSPEEMPKAAALAYGAPLDRRVADTQS
                     CIDELKDKGPVFAVGYCYGGSVVWAAACRCTGLAAASSYYGKLVPEMADETPKCPTIC
                     HFGKTDASIPLDEVEKLRAKHPEVAVYLYDAGHGFNSERPTHYAEEAATLARERTLEL
                     FRANGG"
     gene            6635..8839
                     /locus_tag="VGC27_06275"
     CDS             6635..8839
                     /locus_tag="VGC27_06275"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0282212.1"
                     /translation="MRRGYIVLVVAILFAAWRLQTPTPAPIDAPAASFSASRAFADIR
                     AIAQRPHPVGTADHDRVRDYLVRRLTEMGLAPQLRPDLSTTDFIPGQTVISPVTNIVA
                     VLKGKDREKPALLVMSHYDSVPNSPGAADDSAGVAASLEIARALNSAPQPERDVLFLV
                     TDGEELGMMGATAFFERDPLARHVGVVINFEARGDAGLTAMFETGPMNAGTVAMYAAG
                     ASRPSANSLSRAIYKRMPNGSDFTQAVKRDLPGLNFAFIGDELAYHSPLATPAHLDLG
                     SVQHMGDQALAAARAFAAALPEQKADAVYSDILGFFFIQYSFAVGWVLFAVAALLAFY
                     AIWAAWRVERPSWWRGLAGTLLIVLLPVLLLGLAGYSFRGVDHFQRLSHTGFLLAGAV
                     TLAIGSVALIAALFTRPTSRPPALWQTMLLLLLLLAGAAQAFLPEAAFTLVWPLLVTA
                     LVAAFRFGLYRGANGLPSAAVATLAALIVFSQTAYSAAFLFTAVGVDIPVVLAMPLLS
                     IVPLLLLLADSKPLPLWTHAVVIAAGVALFAYGRLAAPTPERPGPSFVRYVRDLDDGK
                     AYRVAAFNALDPWTEAALGSPRFSALPWSNGAKSWWAPAEPAGVPPSSLAIERDGAQL
                     RIRVRTPPGAFSAAVALRSSEALGPSSFDGMKIAALTAGGGHEVRYFAPAPGDHIWVL
                     EAPRRGSIDVKLTTTYLDWPKDVAKLPPMPADRMAFGTSGGTQTVLRRTWRP"
     gene            complement(8874..>9825)
                     /gene="ychF"
                     /locus_tag="VGC27_06280"
     CDS             complement(8874..>9825)
                     /gene="ychF"
                     /locus_tag="VGC27_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY0282213.1"
                     /translation="ARLDKLSEIAKSAQTVPTRITFVDIAGLVRGASKGEGLGNQFLA
                     NIREVDAIVHVLRCFEDDDVTHVEGRIDPVSDAEVVETELMLADLESLEKRVKPLEKK
                     AHSGEKEAKEQLALMMKAVDLLREGRPARQAELTPEQREPFRLLGLLTQKPVLYVCNV
                     DEASAATGNTFSKRVEDMAKREGAGAIAISCRIEEEVAQLAPAERAEFLASLGLEEPG
                     LNRLIRAGYDLLGLVTFFTAGPKEAKAWTVTKGARAPQAAGVIHTDFEHGFIRAETFA
                     YEDYVAFGGEAGAREAGKFRLEGKDYVVQDGDVMHFRFSV"
BASE COUNT         1571 a         3184 c         3111 g         1959 t
ORIGIN      
        1 cggaatctga acatctggta cgggttcggc gtcatcctca ccttcttcct gggcttgcag
       61 atcgtcagcg gtgtggtgct ggcgatgcat tacgtggcca gcgcggatct cgctttcaat
      121 tccgtcgaga agatcatgcg cgacgtgaat tacggctggc tgatccgtta cctgcacgcc
      181 aacggcgctt cgatgttctt cctcgccgtt tatatccaca tgttccgcgg cctttattac
      241 ggctcctaca aggagccgcg cgaagtcctc tggattctgg gcatgctgat ctatgtgctg
      301 atgatggcga cggccttttt cggctatgtg ctgacctggg ggcagatgtc gttctggggc
      361 gccaccgtca tcaccagcct gttcagcgcg attccctatg tgggcgacgc catcaccgct
      421 tggctgtggg gcggtttcgc ggtcggcgac gccacgctga accgcttctt ctcgctgcat
      481 tatctcctgc catttgcgat cgcggccgtg gtcggcctgc acatctgggc gctgcatgtg
      541 cccggcaaca acaatccgct cgggatcgac gttcaagggc cgcaggacac cgtgccgttc
      601 catccctatt acacggtgaa ggacgcgttc tatctgtcgc tgttcctcat cctgttcgcg
      661 ggctttgtgt tcttcgcgcc ggactttttc ggcaatcccg acaattacac cccggccaat
      721 ccgctggtga ccccgccgga catcgtaccg gaatggtatc ttttgccgtt ctacgcgatg
      781 ctgcgcgcgg tgccgcagaa gctgatcggc gtgctggttc ttgtaggcgc tctggtgatt
      841 ccctttatcg tgccgtggct ggatacttcg aaagtgcggt cctgccgctt ccgcccgctg
      901 atgaagccgt gtttctggct gttcgtgctc gactgcgtgc tgctcggcta tctcggctcg
      961 cagggcgcgg acgcggcgtt cgatctcggc ggcatgcagc tgccgctggt atggctggcg
     1021 cgattgggca cggcctacta cttcgcttat ttcttggtac tgatgccgct ggtaggcctg
     1081 atcgaaaagc ccaaacccct gcccgaaagc atcgcccggt ccgtgctcgg cgccaaggga
     1141 gaacggacat gaaaatcgcg cttccgctct tcgtcctggc gttcggcctt tcgtttggca
     1201 gcgcggccgc cgacaacagc gcggcgctgc cccccaggca catgcaatgg tcgttcgccg
     1261 ggccgttcgg cacctacgac cgcggcgcgc tgcaacgcgg cttcaaggtc tacaaagaag
     1321 tttgcgcggc ctgccacggc ctggacctcg tcgcttttca cgatctggca aatccgggcg
     1381 gccccggctt taccgaggcg caggccaagg cgatcgcggc cggctacagg attccggccg
     1441 aacccaacga caagggcgag attttcgatg ccaacggcaa ccgtctgacg cggccgggca
     1501 ttctcgccga ccgttttccg ccgccgttcc ccaacgaaga agccgcgcgc gcgtccaata
     1561 acggctcgct gccgcccgat ctgtcgctga tcgccaaggc gcacgtcggc gggccggatt
     1621 acctctattc gctgctggtc gggttcggtc acaagccgcc caaggacttc aaggtgctgg
     1681 agggcaagta ttacaacccc tatttcgcgg ggcggaacat cgccatgccg ccgccgttaa
     1741 tggccggctc ggtcagctat cccgacggca ccaagaccag cgtggcgcag gaagcgcacg
     1801 acgtcgttac cttcctggcc tgggcggcgg aacccaagct ggaagagcgc caccgcatcg
     1861 gcttccaggt gatggcgttc ctggtgctgc tggcggggtt actgttcctg acctaccgca
     1921 aggtgtggaa gggcgagcac tgagctattt tgcctccccc cttgaacccg gctacgccaa
     1981 ggcttcgccg ggccaagcgt gaacccgccg aagctatgcg aaggcgggtt gagggggagg
     2041 cggaaaatct gcgaagcgga ttttcggtgg gggtaatatc gtcaataccc ctccccgaaa
     2101 tttgcgaagc aaatttcgac cctccctcaa ggggagggtg ggattcctca ctacacatgc
     2161 taaaaccctc ctttccctga gggcaggtca tgagcagaac agttctcggc atcatcggcg
     2221 gcagcggcgt ctacgacatc gggggtcttg aaaatcctcg ctgggagcgg gtggaaggcc
     2281 cctggggcga gccttcggac gagcttttgt taggcaccta tgcgggtgtg gacttggtgt
     2341 tcctgccgcg ccacggccgc ggccatgtcc ggacgccgag cacggtcaac taccgcgcca
     2401 atatcgacgc cttgaagcgg gcgggggtta ccgacgtgct ctcggtgtcg gcctgcggtt
     2461 cgctcaagga ggagctggcg ccgggcacct tcgtgttcgt cgaccagttc atcgaccgta
     2521 ccttcgcgcg cgaaaagact tttttcgggc cgggattcgt ggcgcacgtc tcgatggccc
     2581 atccggtctg tccgcggctg accggtgcgc tcgcaagtgc ggcgcgcgag aaaggcatcc
     2641 gccacgccag gggcggcacc tatatttgca tagaagggcc gcaattctcg acgctcgccg
     2701 aatccaatct ctatcgttcc tggggttgct cggtggtcgg catgacggcg atgccggaag
     2761 ccaagctggc gcgcgaagcc gagctgcctt atgcgctggt cgccatggtc accgatttcg
     2821 attgctggca tcccgatcgc gatcatgtga cggtcgacgc ggtggtcaag gtcctgctcg
     2881 ccaatgccga acaggcgcgc aagctgatct gccaggccgc gaccaagctg gggccggagc
     2941 gctcgccgtc gccgctcggc atcgagaacg cgctcgatac cgccatgatc accgcacccg
     3001 aaaagcgcga tccgcaactt atcgccaagc ttgacgccgt ggcggggagg gtgctgtcca
     3061 aggtcgaggc cgatccggct ggccaataaa caggtatttg catgaggcgg catgaccgga
     3121 tggccgggtc gcgctacgct tcgcttgctg cccggccatg acgagggatc aaggcatgga
     3181 cttcgcatcc gtcatccgca ccattccgga ctatcccaag aaagggattc tgttccgcga
     3241 cgtcaccacc ttgttcggca atccgcgcgc cttccgcgcc gcggtggacg ccctggtgca
     3301 gccttacgcc ggcgtgcgca tcgacaaagt ggccggcatc gaagcgcgcg gcttcatcct
     3361 gggcggcgcg gtggcgcatc agctttccac cggcttcgtg ccggtgcgca agaagggcaa
     3421 gctgccctac aacgtgatcg gcgaggaata cgatctcgaa tatggcaagg accgggtgga
     3481 aatccacgtc gatgcggtga agaagggcga gtatgtcgtg ctggtggacg atctgatcgc
     3541 caccggcggc accgccttcg ccagcatcaa gctgctggag cgcgccgggg caaaggtcgt
     3601 gggctgcgct ttcgttatcg atcttcccga actcggcggc gccgacaaaa tccgcgccct
     3661 gggaaaggaa gtgacgacct tggtaagttt tgaaggacac tagagcgtga tccgatttaa
     3721 ttgaatcgga tcaagctcta gattccttgg ttttcgcgca atctgacggc aaaccgcgtc
     3781 ggcacgctgc tgctaccgac cactttgccg gattgcgctc tagcttttta ccctcccctt
     3841 gagggagggt cgaagcgcga agcacttcgg ggaggggtcg tggcccttac ccctccccga
     3901 aaaatctttt gcttcgcttg gttttttcgg ccctccctca aagggagtgt ggaaaggtac
     3961 ctatgaagct ctacatgtcg ccggcttctc cttttgcccg caaggcgcgc atcatcatcc
     4021 gcgagctcga tctgacgcgg ctggtggagg aaatcccgac cgatcccgcg accagcggtg
     4081 agctgcgcaa gaccaatccg ctggggaaaa tcccggtgct ggtgctggac gacggctcga
     4141 cgctgatcga ttctcccgtg atctgcgaat atctgaacga aatgggcggc ggaaagttct
     4201 ttcccggcat gagcatctgg cgctcggctt ccgggcgctg gcgggccctc accctgcaag
     4261 cgctgggcga cggcctcgcc gaagcggcgg tggcgcgcct ctatgaaaga cgccgccccg
     4321 aggaattgca gaacaaggag gcgatcgcca agtatctggc cgcgatcggc cgttcgctcg
     4381 atgtgctgga gcgcgcgcgc ttcgccaagc cgccgaccat cggcgagatc gcggtggctt
     4441 gcgccatcgg ctatctcgat ttccgcgagg tgatgcccgg ctggcgcgac atgcgtccgc
     4501 aattgcgcgg ctggtatgag gatttcgtca aataccctgc catgcaggaa acttggccgg
     4561 cgaacctgac gtgaagattg cgggcagaca ttaccgtacg atttggccgc gcgccgacga
     4621 ggcggtggag gtcatcgacc agacccgtct gccgcacgca ttcgccaccg ccgttctgcg
     4681 taccgccgag gattgcgccc aggcgatccg cagcatggtg gtgcgcggcg cgcccttgat
     4741 cggtgccacg gcggcttacg gcgtcgcctt cgccatgcgc gccgagcctt cgagcaaggc
     4801 tttggtagcg gcgctcgacc tgttgcaagc gacgcggccg acggcggtga atctgcgctg
     4861 ggcgctgaag cgcatggcgg gcgtgctgca aagccgatcg cacggcgacc gctttgtgct
     4921 cgcctggaac gaagccgccg cgatctgcga cgaggatgtg gaggtctgcc gctccatcgg
     4981 cgaacacggg ctgaaacttc tgcgcgaggc ggcggcgaag aagaacggtg ccccgctcga
     5041 tatcctcacc cattgcaacg ccggctggct ggcctgtgtt gattggggca ccgcgctggc
     5101 gcccatctac atggcgcatg atgcgggcat ggacgtgcat gtgtgggtgg acgaaacccg
     5161 cccgcgcaat cagggcgctt cgctgacggc gtatgaactg ggcgcgcacg gcgtgccgca
     5221 caccatcatc accgacaatg ctggcggcca ttatatgcag acggggcgcg tggacctttg
     5281 cttcgtcggc accgaccggg tggcgatgaa cggcgacgtc gccaacaaga tcggcactta
     5341 tctgaaagcg ctggcggcga aggacaataa cgtcccgttc tacgtggcgc tgccgtcgtc
     5401 cagcatcgac tggacgctgg cgtccggaac ggaaatcccc atcgaagagc gctcgcccga
     5461 cgaagtgctg aagatgacgg ggcgcttgcc agatggcacg atagcgacgg tagacatcgc
     5521 tgcgcccggt tcgcccgccg ccaatcccgg cttcgacgtg acgcctgcgc ggctggtgac
     5581 gggcttcgtc accgaacgcg gcatctgcgc cgcgagcgaa gcaggattgc gcggcctttt
     5641 tccggcttag tttctgcccc cgccctgccc accgaagctt tagcgtaggt agggagcggg
     5701 ggaagtgccc accgaagcct tggcgaaggt gggcgaaggt gggcgaaggg ggttatcccc
     5761 cgttcgcccg gaacaattcc agcgtccgct cccgcgccag ggtggcggcc tcttcagcat
     5821 agtgcgtcgg ccgttcggaa ttgaagccgt ggccggcatc atagagatag accgccactt
     5881 cgggatgctt ggcgcgcagt ttttccacct cgtcgagcgg aatgctcgcg tcggttttgc
     5941 cgaaatggca gatcgtcgga catttgggcg tttcgtccgc catctcgggc accagcttgc
     6001 cgtaatagct ggaggcggcg gccagccccg tacagcggca agcggcggcc cacaccaccg
     6061 agccgccata gcaatagccg acggcgaaca ccgggccttt gtctttcaac tcgtcgatgc
     6121 agctttgcgt atcggccacg cgccggtcga gcggcgcgcc ataggccagc gccgccgcct
     6181 tcggcatttc ttctggcgaa tagccggaaa tgaatccggg cgcctcgcgg tcgaacagcg
     6241 agggcgccag cacctcgtag ccttcggcgg cgtaggaatc agaaaccgcc ttgatgtgtc
     6301 cggtgacgcc gaagacttcc tggatcagca ccagcccgcc gcgccgcacg ccgtgcggct
     6361 gcacgcgata gacgccgatg gctttgccgt cggacatcgt caggaaaatc atcttgcctg
     6421 ccgccatgtt gcgtctcctt ggctaccggc catttcagcg gacgcggcag gacgtggcaa
     6481 gtgattcgcg cgtcgttttg ccgcttccac cgcaagcagg aggaaagccg cgcgcccgct
     6541 tcgccgccga tcgcggcttc ggcgtaacgc cggcgtgttt tggcccatat cctccccgat
     6601 gctattctgc ccattgcatc tttggggggc ggccatgcgc aggggctata tcgttttggt
     6661 ggtggcgatc ttgtttgcgg cttggcggct gcagacgccc actccggcac ccatcgatgc
     6721 gcccgccgct tcattctccg cgtcgcgcgc cttcgccgac atccgcgcca tcgcgcaacg
     6781 cccgcatccg gtcggcacgg ccgaccatga tcgggtgcgg gactatctcg tccgccgtct
     6841 gaccgagatg ggcctcgccc cccagttgag gccggacctc agcacgacgg atttcatccc
     6901 tgggcagacc gtcatctcgc cggtgaccaa catcgtggca gtcctcaagg gcaaggatcg
     6961 cgagaaaccc gctttgctgg tgatgagcca ttacgactcg gtgcccaatt ctcccggcgc
     7021 cgccgacgac agcgccggcg ttgccgcctc gctggaaatc gcccgcgcct tgaactccgc
     7081 cccgcagccg gaacgcgacg tcctgttcct cgtcaccgac ggcgaagagc tgggcatgat
     7141 gggggcaacc gcattcttcg aacgcgatcc gttggctcgt catgtcggcg tggtgatcaa
     7201 cttcgaggcg cgcggcgatg ccggcctgac tgcgatgttc gagacgggcc cgatgaacgc
     7261 cggcacggtg gcgatgtacg ccgcgggggc gtcgcggccg agcgccaatt ccctgtcgcg
     7321 cgccatctac aagcgcatgc ccaacggctc ggatttcacg caagcggtga agcgcgatct
     7381 gcccggcctc aacttcgctt tcatcggcga tgaattggcc tatcactcgc cgctcgcaac
     7441 gccggcccat ctcgatctcg gcagcgtcca gcatatgggc gatcaggccc tggcggcagc
     7501 ccgcgccttc gccgccgcgc tgccggagca aaaggcggat gccgtctatt ccgatatcct
     7561 cggctttttc ttcatccaat attcctttgc ggtcggctgg gtcttgttcg cggtcgccgc
     7621 tttgctggcg ttctatgcga tttgggcggc ttggcgcgtg gagcggccgt cctggtggcg
     7681 cggccttgca gggacgttgc tgatcgtttt gctgccggtg ctgctgttgg gtctggccgg
     7741 atattcgttc cggggtgtcg atcattttca acgcctctcg cacaccggct tcctcttggc
     7801 gggcgcggtg accttggcga tagggtcggt cgcgctcatc gccgctctat ttacacgccc
     7861 cacatcacgc ccgcccgcct tgtggcagac catgctgctg ctccttttgc ttctggcggg
     7921 cgcggcgcag gcgttcctgc ccgaagccgc cttcacgctg gtctggccgc ttctcgtcac
     7981 ggccttggtt gccgcgtttc gcttcggcct ctatcgcggc gctaacggcc ttccttcggc
     8041 cgccgtcgcg accctggccg ccttgattgt cttttcgcaa accgcttaca gcgccgcctt
     8101 cttgttcacg gcggtcggtg tcgatattcc cgtcgtgttg gcgatgccgc ttctgagcat
     8161 cgtcccgctt ttgcttctgc tggcggactc aaagcccctg ccgctctgga cccatgccgt
     8221 cgtgatcgcg gcgggcgtgg cgctgttcgc ctatggacgg ctggccgcgc cgacgcccga
     8281 gcggcccggc cccagcttcg ttcggtatgt tcgggatctc gacgacggca aggcctatcg
     8341 cgtggcagcc ttcaatgcgc tggacccttg gaccgaggcc gcactcggct cgccgcgctt
     8401 ttccgccttg ccgtggagca atggcgcgaa atcttggtgg gcgccggcag agccggccgg
     8461 cgttccgcct tcgtctctcg ccatcgagcg cgacggggcc caactgcgga ttcgggtccg
     8521 caccccaccg ggcgcctttt ccgccgccgt ggcgttacgc tcgtccgagg cgctcggccc
     8581 aagcagtttc gacggcatga agattgccgc gctcacggcc ggcggcgggc acgaagtccg
     8641 gtattttgca ccggctccgg gcgatcacat ctgggtgctg gaggcgccgc gccgcggcag
     8701 catcgatgtc aagctcacga cgacctatct cgattggccc aaagacgtgg ccaagctgcc
     8761 gcccatgccc gcagaccgca tggctttcgg cacatcgggc ggtacgcaga cggttctgcg
     8821 gcgtacctgg cggccttaga gcgcgatccg cttcaactaa atcgggtcgc gttctaaacg
     8881 ctgaaccgga aatgcatcac gtcgccgtcc tgcacgacgt aatccttgcc ttccaggcgg
     8941 aatttcccgg cctcgcgcgc gcccgcttcg ccgccgaagg cgacataatc ttcgtaagca
     9001 aaagtttcgg cgcggatgaa gccgtgttcg aaatccgtat ggatgacgcc cgccgcctgc
     9061 ggcgcgcgcg ctcccttggt caccgtccag gctttcgctt ccttcggccc ggcggtgaaa
     9121 aaggtgacga ggccgagcag gtcgtagccc gcgcggatca agcggttcaa accgggttct
     9181 tcgaggccga ggctggcgag gaattcggcg cgctcggcgg gcgcaagctg cgccacttct
     9241 tcctcgatgc ggcaggaaat cgcgatcgcg cccgcgcctt cgcgcttggc catgtcttcg
     9301 acgcgcttgg agaaggtgtt gccggtcgcc gccgaggctt cgtcgacgtt gcagacataa
     9361 agcacgggtt tctgcgtcaa caggccgagc aggcggaaag gctcgcgctg ttccggggtc
     9421 aactcggcct gtcgcgccgg cctgccctcg cgcaacagat cgaccgcctt catcatcagc
     9481 gccaattgtt ccttggcttc cttctcgccg gagtgcgcct tcttctcgag cggtttgacg
     9541 cgcttttcca gactttcgag gtccgccagc atcagctcgg tttccaccac ctcggcgtcg
     9601 ctgaccggat cgatgcgccc ttccacatgg gtgacgtcgt cgtcctcgaa acagcgcagc
     9661 acatgaacaa tggcgtccac ttcgcggatg ttggcgagga actgattgcc cagtccttcg
     9721 cccttggaag cgccgcgtac cagcccggcg atgtccacga aggtgatgcg cgtgggcacg
     9781 gtctgggcgg atttggcgat ctcgctcagc ttgtcgagcc gcgcg
//
LOCUS       DASWPU010000294         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_100682,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000294 DASWPU010000000
VERSION     DASWPU010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100682"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VGC27_06285"
     CDS             complement(<1..262)
                     /locus_tag="VGC27_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0282214.1"
                     /translation="MKPSILVAEDEGALITLLRYNLEREGYRVLEAQDGEEALLVAAE
                     EKPDLVLLDWMLPQLSGIEVCRRLRGRQETRNVPIIMLTARGE"
     gene            complement(265..975)
                     /gene="phoU"
                     /locus_tag="VGC27_06290"
     CDS             complement(265..975)
                     /gene="phoU"
                     /locus_tag="VGC27_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY0282215.1"
                     /translation="MTDHTVKAFTEQLEALASSVAQMGGLAEAQLADAIDAIARRDTG
                     RAERVIGGDQRVDELQQQIEDKALKLLALRQPMAVDLRETLAALKCAGELERIGDLAK
                     NIAKRAIVLNREPPIRLTQSLARMGGQALSQLKLVLDSYSDRDADAAEAVWRQDGEID
                     EMYNSLFRELLTYMMEDPRTIGLCTHLLFVAKNIERTGDHATNIAEVVFNMVTGGHLA
                     TDRPKADITSETSFEKKA"
     gene            complement(1048..>2083)
                     /locus_tag="VGC27_06295"
     CDS             complement(1048..>2083)
                     /locus_tag="VGC27_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0282216.1"
                     /translation="PDPLLLVDTAGRVLFVNRAMREVIGVGAERKHVSSLLRTPTVLE
                     ALARTSASGEAGTVEFTFRVPVERHYQAYTARIGSDPVVTTLLLHDLTAIKRAEQMRV
                     DFIANASHELRTPLAAVTGFIETLKGPAKDDLAAREKFLGIMGVEAARMRRLIEDLLS
                     LSRIELNEHVLPQGEVSVEAIVREAVTALTPLAEADGITISIDAAEGLPKVVGERDEL
                     IQLFQNLIHNAIKYGRDNGQITISLRQMPSAGRRGSGALVVASVRDDGEGIPPEAIPR
                     LTERFYRVDVKRSRERGGTGLGLAIVKHIVNRHNGRLTIESRAGEGSTFTVSLPAGRK
                     PVDTAVTEVL"
BASE COUNT          337 a          676 c          670 g          400 t
ORIGIN      
        1 cttcgccccg cgccgtcagc atgatgatcg gcacgttgcg cgtttcctgg cgcccgcgca
       61 ggcgacggca cacttcgatg cccgaaagct gcggcagcat ccagtcgagc aggacgagat
      121 cgggcttttc ttccgccgcg accagcaacg cttcctcgcc gtcctgcgct tccagcacgc
      181 gatagccttc gcgctccaga ttgtagcgca gcagcgtgat cagcgcgcct tcgtcttcgg
      241 caaccaggat cgacggtttc atggtcaggc cttcttctca aaactcgttt cgctggtgat
      301 gtcggctttc ggccggtcgg tggcgaggtg accgcccgtc accatgttga aaacgacttc
      361 ggcgatattg gtggcgtgat cgcccgtgcg ctcgatgttc ttggcgacga agagcagatg
      421 agtgcaaagc ccgatggtgc gcggatcttc catcatgtag gtgagaagtt cgcggaacag
      481 gctgttgtac atctcgtcga tttcgccgtc ctggcgccac acggcttcgg cggcatcggc
      541 atcccggtcc gaataggaat cgagcacgag cttgagctgg ctcaaagcct gtccgcccat
      601 gcgcgccaga ctttgagtca agcggatcgg cggctcgcgg ttgagcacga tggcgcgctt
      661 ggcgatgttc ttggccagat cgccgatacg ttcgagctcg cccgcgcatt tgagcgcggc
      721 caaagtctcg cgcaggtcca ccgccatggg ctggcgcagg gccagcagct tcaacgcctt
      781 gtcctcgatc tgctgctgca actcgtcgac gcgctggtcg cccccgatca ctctttccgc
      841 ccgccccgtg tcgcgccgcg caatggcgtc gatggcatcg gcgagctggg cctcggccag
      901 cccgcccatc tgggcgaccg aagaggccag ggcttccaac tgctccgtga acgctttcac
      961 cgtatggtcg gtcatgattg cctgtccggt tcgtggcagc ggcggtacat tgatttaggc
     1021 gcaaaccgcc atggctatgc gacggtttta taacacttcc gtgacagccg tatcgactgg
     1081 tttgcggccg gccggcaggg aaaccgtgaa ggtactgccc tccccggccc ggctttcgat
     1141 cgtcagcctg ccgttatggc ggttgacgat gtgcttaacg atggcgagac ccaggcccgt
     1201 gccgccacgt tcccggctgc gcttcacatc gacgcggtag aaacgctcgg tcaggcgcgg
     1261 gatggcctcc ggcgggatgc cctcgccgtc gtcgcgcacg ctggcgacaa ccagcgcccc
     1321 ggagccgcgc cggccggcgg acggcatctg gcgcagcgag atcgtaatct gcccgttgtc
     1381 gcggccgtat ttgatggcgt tgtggataag gttctggaaa agctggatca gctcgtcgcg
     1441 ctcgccgacc accttcggca ggccctcggc ggcgtcgatc gaaatggtga ttccgtccgc
     1501 ttccgccaag ggcgtcagcg cggtgacggc ctcgcggacg atggcctcga cggaaacctc
     1561 gccctgcggc aggacgtgtt cgttgagttc gatcctcgac aaggacaaga gatcctcgat
     1621 cagccggcgc atgcgcgccg cctccacccc catgatgccg aggaatttct cgcgcgccgc
     1681 caaatcgtct ttcgccgggc ctttcaaggt ttcgatgaag ccggtgacgg cggccagcgg
     1741 cgtgcgcaat tcgtggctgg cattggcgat gaaatccacg cgcatctgct cggcgcgctt
     1801 gatcgcggtc aggtcgtgca acagcagcgt ggtgacgacg ggatcgctgc cgatgcgcgc
     1861 cgtataggcc tgatagtgac gctcgacagg cacgcggaag gtgaattcaa ccgttcccgc
     1921 ctcgccgctg gccgaggtgc gcgccagagc ctcgagcacg gtgggcgtgc gcagcagcga
     1981 agagacgtgc ttgcgttcgg cgccgacgcc tattacctcg cgcatggccc ggttgacgaa
     2041 cagcacgcgg ccggccgtat cgaccagcag caagggatcc ggc
//
LOCUS       DASWPU010000295         3924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_100687,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000295 DASWPU010000000
VERSION     DASWPU010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100687"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..395)
                     /locus_tag="VGC27_06300"
     CDS             complement(84..395)
                     /locus_tag="VGC27_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY0282217.1"
                     /translation="MSRKIIKRLDPIPPGEILFEEFMKPLGVSQNRLARDIDVNPARI
                     NDIVHGRSSITASIALRLSKYFGTTPELWMNLQSSYELRKARAADWPAAEHRVRVLAA
                     E"
     gene            complement(404..682)
                     /locus_tag="VGC27_06305"
     CDS             complement(404..682)
                     /locus_tag="VGC27_06305"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0282218.1"
                     /translation="MIRSFRCADTGRLFADEDVPRFANFGRVARRKLLYLDAATILSE
                     LRSPPGNRLEALKGDRKGQHSIRINDQWRVCFVWRNGGAEDVEIVDYH"
     gene            complement(791..1390)
                     /gene="phaR"
                     /locus_tag="VGC27_06310"
     CDS             complement(791..1390)
                     /gene="phaR"
                     /locus_tag="VGC27_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY0282219.1"
                     /translation="MAEESSKPQAPVVIKKYANRRLYNTQTSSYVTLDHLCEMVKEGV
                     EFEVIDARSGEDITRSVLTQIIFEEETKGHNLLPIRFLRQLIRMYGDSMQAFVPGYLD
                     LSMESFTKNQEAMRSRIAEAFGGGSQALENLTRQNLAMFERAMQMFTPFAPRAAHPED
                     GEPRPNGAADVKPSEEISELKSEIEVMRRQLAELSQQRK"
     gene            1828..2445
                     /gene="ccmA"
                     /locus_tag="VGC27_06315"
     CDS             1828..2445
                     /gene="ccmA"
                     /locus_tag="VGC27_06315"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEY0282220.1"
                     /translation="MNLALEVRDLACIRGQRLLFRALNFSLAAGRALSLQGPNGVGKT
                     SLLRLLAGFLRPAAGTVCLRINGTEAADAEERGKFIGWLAHQDAVKPQLGVREQLAFF
                     ARLYGSGRDLGEAMRSFGLEPLAELPGQYLSAGQKRRLALARLQLSARPVWLLDEPLA
                     ALDAAGRRLVAETVTAHCASGGIVVAATHEELGVECETLRLGERS"
     gene            2597..3262
                     /gene="ccmB"
                     /locus_tag="VGC27_06320"
     CDS             2597..3262
                     /gene="ccmB"
                     /locus_tag="VGC27_06320"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY0282221.1"
                     /translation="MIPFFALLFRDIRLAVRAGGGAALALAFFACVVTLVPLGVGADL
                     RLLARIAGGVLWVAAVLAALLALDRLFQADYEDGSLDLIALSPLPLEVTALAKTAAHW
                     LATGLPLTVLSALLAVLFDLSLRATGALVLSLLIGTPAVSSLGAVGAALTLSIRRGGL
                     ILPLIVLPLLAPAVIFGSGAVLAVEDGLADGALYFLAAFSLIAVALSPFAVAACVRLN
                     LAA"
     gene            3307..>3924
                     /locus_tag="VGC27_06325"
     CDS             3307..>3924
                     /locus_tag="VGC27_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEY0282222.1"
                     /translation="MSMFELANPKRFMQVSGKFLPWIAAAAATTLAAGLVLGFAAPPD
                     YRQGDTVRIMFLHVPAAWTAMMAYALMALASAVGLINRHPLADVAAKTAAPLGAVFTA
                     LGLITGSLWGKPMLGAWWVWDGRLTSFLLLLLLYLGYIALGSAIEDENRSARACAILA
                     IVGAVNLPIIEFSVDWWTTLHQGQSILRKGGPSIAPVFLRPLLLMG"
BASE COUNT          604 a         1237 c         1284 g          799 t
ORIGIN      
        1 gaaagcttcg gcatcgacag tttccagggc tattatttcg gcgagccggt gctcaagccg
       61 gaatggatga aatagccgac tccctactcg gcggcaagca cgcgcacgcg atgttcggcg
      121 gcaggccaat cggcggcgcg agccttgcgc agctcatagc ttgattgcag gttcatccat
      181 aattccggcg tcgtgccgaa atacttcgac aagcgaagcg cgatggacgc cgtaatcgat
      241 gagcggccat gcacaatgtc gtttatgcgc gccggattga cgtctatgtc gcgcgccaag
      301 cggttctggc tcacgcccag cggtttcatg aactcttcga aaagaatctc gccgggagga
      361 atcggatcga gacgctttat gattttacgc gacatgtggg acctcaatgg taatccacga
      421 tttcgacatc ttccgctccg ccgttacgcc agacgaaaca cacgcgccac tgatcgttga
      481 tccggatcga atgttgccct ttgcggtcgc ccttcaacgc ttcgaggcgg tttccgggcg
      541 gcgatctcaa ttcgctcagg atggtcgccg catccagata gagcagcttc cgacgcgcta
      601 cccggccgaa attcgcaaac cgcggcacat cttcgtccgc gaataggcgt ccggtatccg
      661 cacatcggaa cgaacggatc atgcgcacag gctattctat attagggatt atcgcaagga
      721 gaatagcggc tacgcgctca atggctcccg aatggcctat cggcgtccgg aacaccctgt
      781 tgtcacaagg tcatttgcgt tgctggctga gttcggcgag ctgccggcgc atcacctcga
      841 tctcgctttt cagttcgctg atttcttccg agggcttcac gtcggcggcg ccgttgggcc
      901 tgggttcgcc gtcttcgggg tgtgccgcgc gcggggcgaa gggcgtgaac atctgcatgg
      961 cgcgttcgaa catcgccaga ttctgccgcg tcagattttc gagcgcctgg ctgccgccgc
     1021 cgaacgcctc ggcgatgcgg ctgcgcatgg cttcctggtt cttggtgaag ctctccatcg
     1081 agagatcgag ataacccggt acgaacgcct gcatgctgtc gccgtacatg cggatgagct
     1141 ggcgcaggaa gcggatgggc agaaggttat gccccttggt ctcttcctcg aagatgatct
     1201 gcgtgagcac cgagcgggtg atgtcttcac cgctgcgcgc atcgatgacc tcgaactcga
     1261 cgccctcttt gaccatctcg cagagatgat cgagcgtgac gtagctggag gtctgggtgt
     1321 tgtagagccg ccggttcgcg tacttcttga tgacgaccgg agcttggggt tttgaagatt
     1381 cttccgccac gcgtcaccct gctgaattgt tgagcgagca tcgccggttt ctggctctcc
     1441 ggccacctat gacaacatga catattctgc cctgtccagc cattttgtgc tactgcaaca
     1501 agaaccggca ctggaagtgc cgtttaacca attgaaataa ctacattgtt ttaggtccca
     1561 atgccgcact cgcaggcgcg gcaatttgcg agcggctgca gcaatcgccg gaatcgcgcc
     1621 ggccgcgggg gagcggcggc ttcgacatcg cacgccgagt cccgttcccg gccgcaggcg
     1681 aaggggggat tcgtcaccgg cccggccatg ccgatcgccg gcggacggca catccccaga
     1741 atgccgccgg ccgcgcccgg cttctgttga ccgccggatc gcgactgtgc tcaccatgcc
     1801 ggcccggcat gtgagagaat ctccgtcttg aacttggccc ttgaggttcg cgatctggcc
     1861 tgcatccgcg ggcagaggct gctgttccgc gcgctgaact tttcgctggc ggcggggcgg
     1921 gcgttgtcgc tgcaaggccc gaacggggta ggcaagacct cgctgctgcg cctgctcgcc
     1981 ggattcctca ggccggccgc cgggaccgtc tgcctgcgaa taaacggcac ggaggccgcc
     2041 gatgccgaag agcgcggcaa gttcataggc tggctcgccc atcaggatgc ggtcaagccg
     2101 cagctaggcg tgcgcgaaca gcttgctttc ttcgcccggc tctatggcag cgggcgcgac
     2161 ctcggcgaag cgatgcggag cttcggactc gaaccgcttg ccgaacttcc cggccaatat
     2221 ctgtcggccg gacagaaacg gcgcctggca ctcgcccggc tgcaactttc ggcgcggccc
     2281 gtgtggctgc tcgacgaacc gctggcggcg ctcgatgcgg cgggcaggag actggtggcg
     2341 gaaaccgtca ccgcccattg cgcctcgggc ggcatcgtcg tcgccgcgac gcatgaggaa
     2401 ctcggcgtcg aatgcgagac gctgagattg ggggagcgat cttgagcaca ccgttttttt
     2461 cctcccccgt tcacggggga ggtgctccgc caagcggagc ggagggggcg aacgctgcgc
     2521 cggccccctt cgcccacctt cgccaaggct tcggtgggca ctccccccgt gtacgggggg
     2581 agaaaagggt gccgcagtga ttccgttctt cgctcttctc ttccgcgata ttcgcttggc
     2641 ggtgcgggcg ggcggcggcg cggccctggc gctggccttc ttcgcctgcg tcgtgaccct
     2701 ggtgccgctc ggcgtcggcg cggatctgcg gctgttggcg cgcatcgccg gcggagtgtt
     2761 gtgggtggcg gcggtgctgg cggcgctgct cgcgctcgac cgtctgttcc aggccgacta
     2821 cgaggacggc agcctcgatc tcatcgcctt gtcgcctttg ccgctggaag tcacggcgct
     2881 cgccaagacc gccgcgcatt ggctcgccac cggcttgccg ttgaccgtcc tgtcggcgct
     2941 gctggcggtt ctgttcgatc tctccttgag agcgaccggg gccttggtgc tgtcgctgct
     3001 gatcggaacg cccgcagtca gctccctggg cgcggtcggc gcggcgctaa ccctttccat
     3061 ccgccgcggc ggcctgatcc tgcccttgat cgtattgccg ctgctggcgc ccgcagtgat
     3121 cttcggcagc ggggcggtgc tggcggttga ggacgggctg gccgacggcg cgctttattt
     3181 tctggcggcg ttttccctga tcgcggtggc gctgtcgccg ttcgccgtgg cggcctgcgt
     3241 gcggctcaat ctggcggcgt gagagcggca gttgccgcgt ccgccgcgac ggcatagagg
     3301 aacagcatgt cgatgtttga actcgccaac cccaagcggt tcatgcaggt gagcgggaag
     3361 ttcctgccct ggatcgcggc tgcggccgca acgacgctgg cggcgggctt ggtgctcggc
     3421 tttgccgcgc ctcccgatta ccggcaaggc gacacggtcc ggatcatgtt cctgcatgtg
     3481 cccgccgcct ggacggcgat gatggcctac gcactgatgg cgctggcatc ggcggtcggc
     3541 ctgatcaacc gccatccctt ggccgacgtg gcggcgaaga cggcggcgcc tctgggagcg
     3601 gtattcaccg cgcttggtct gatcaccggc tccttgtggg gcaagccgat gttgggcgca
     3661 tggtgggtat gggacgggcg gctgacgtcg ttcctgctgc tgctgctgct ttatctcggt
     3721 tacatcgcgc tcggcagcgc catcgaagac gaaaaccgct cggcgcgcgc ctgcgccatc
     3781 ctggcgatcg tcggcgcggt gaatttgccg atcatcgaat tctccgtcga ttggtggacg
     3841 accctgcacc agggccaaag catcctgcgc aagggcgggc cgtcgatcgc gccggtcttc
     3901 ctccggccgc tattactgat gggc
//
LOCUS       DASWPU010000296         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_94368,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000296 DASWPU010000000
VERSION     DASWPU010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94368"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VGC27_06330"
     CDS             complement(<1..1110)
                     /locus_tag="VGC27_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY0282223.1"
                     /translation="MPAFPTYGPDEIAALDKQAAAILGVFAARGYSRREPAILQPAQI
                     FVDRSGEEIRRRTFELTDPSGRELCLRPDLTIPTCKWQTQSGGPYPARLCYHGLAFRH
                     RPGDGPTQFYQAGAELLGLEDRAAGDVEMMALAVEALRAAGLAGFEMKIGDLGLFATL
                     VDALDVPAQWRGRLKRHFWRAGYFDALLARMVAGAASDAERAIDALHGLGAADLRPAI
                     EALLDKEGDAPLGARSREEIVERLMVQAADGDAPKLDAKVAETIKLVLGVSGLASGAL
                     EQIRTLTKPVAAKMEKPLAAMEARLAALKSLGVDPASVRFVAHFGRNLEYYTGFVFEL
                     WTRDPGDPVEIAGGGRYDTLMESLGAPRGTTAIGCA"
     gene            complement(1401..>1988)
                     /locus_tag="VGC27_06335"
     CDS             complement(1401..>1988)
                     /locus_tag="VGC27_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY0282224.1"
                     /translation="IDPTIIRGLDYYTGPVFEAQLTFPVTNEAGETVVFGSVAGGGRY
                     DDLVARFTGQQVPATGISIGVSRLLTALQSRGLTGDEQKLIVVLSMGGAAQSFATARE
                     LRAAGFRAESYVGTKKIPDQLKYADRRGAALAVMEGEDERAKGEITLKDLKLGAEISK
                     SVESRAEWVGARAAQVTVKRSDLVAEVRNLLSRGG"
BASE COUNT          348 a          678 c          619 g          343 t
ORIGIN      
        1 ggcgcagccg atggcggtgg tgccgcgcgg cgcgcccagc gattccatca gcgtgtcgta
       61 gcgcccgccg cccgcgattt cgaccgggtc acccgggtct cgcgtccata actcgaaaac
      121 gaagccggtg taatattcga ggttgcgccc gaaatgcgcc acgaaacgga ccgaggcggg
      181 atcgacgccc agcgatttca gagcggcaag gcgcgcctcc atcgccgcca gcggcttttc
      241 cattttcgcc gcgaccggct tcgtcagtgt gcggatttgt tccagcgccc cggacgccag
      301 tccgctgacg cccagcacaa gcttgatcgt ctcggcgacc ttggcgtcca gcttgggcgc
      361 atcgccgtcg gcggcctgga ccatcagccg ctcgacgatc tcctcgcggc tgcgcgcgcc
      421 cagcggcgca tcgccttcct tgtcgagcag cgcctcgatg gcgggacgca agtccgccgc
      481 gccgagaccg tgcaaggcat cgatcgcgcg ttcggcgtcg ctggcggcgc ccgcgaccat
      541 gcgcgccaga agcgcgtcga aatagcccgc gcgccagaaa tggcgcttca accgtccgcg
      601 ccattgcgcc ggaacatcga gcgcatcgac cagcgtggcg aacaggccga gatcgccgat
      661 cttcatttcg aacccggcaa gaccggccgc gcgcagggct tccaccgcca gcgccatcat
      721 ttcgacgtcg cctgcggcgc ggtcttccaa gccgagcaat tccgcgcccg cctgatagaa
      781 ctgcgtgggg ccgtcgccgg ggcgatggcg gaacgccagc ccgtgatagc agagccgcgc
      841 cggataaggc ccgccgctct gtgtctgcca tttgcaggtc gggatagtga ggtccggccg
      901 caggcaaagc tcgcgcccgg aaggatcggt gagttcgaac gtccgcctac ggatttcctc
      961 gccggaccgg tccacgaaaa tctgcgccgg ctggaggatc gcgggttcgc ggcggctgta
     1021 gccgcgcgcg gcaaagacgc cgagaatcgc cgccgcctgc ttatccagcg ccgcgatctc
     1081 gtccgggcca taggtgggaa aggcgggcat gaagttagac cgccccccaa tcaaacaaac
     1141 tcaattgttc ttcaccctcc tgaaagaggg tcgaaacggc cctgaacggc tgcgaagggc
     1201 gcgtttcggg gagaggtatt gtcgaggcag tacccctccc cgaaaaatcc ttcgctacgc
     1261 tcgtattttt cgaccctccc tcaacccgtc ttcgctgaag cttcgacggg tagagcgcgt
     1321 agtccggcga agccttggca tagccgggct caaggggagg gtcgaatata tcgcttggtg
     1381 atagaatctt gttcttcacg tcagcctcca cgcgaaagca ggttgcggac ttcggcgaca
     1441 agatcgctgc gcttcaccgt cacctgcgcc gcgcgggcgc cgacccattc ggcgcggctt
     1501 tcgacggact tggatatttc cgcgccaagc ttcaaatcct tgagcgtgat ctcgcccttg
     1561 gcgcgttcgt cctcgccttc catcaccgcc agcgcggcgc cgcgcctgtc ggcgtatttc
     1621 aattgatcgg gaattttctt ggtgccgaca taggattccg cccggaaccc ggcggcgcgc
     1681 aactcacgcg ccgtcgcgaa gctctgcgcc gcaccgccca tcgagagcac aacgatcagc
     1741 ttttgttcgt cgccggtcag tccgcgcgac tgcaatgccg tgagcaaccg gctgacgccg
     1801 atggaaatgc cggtcgccgg gacctgctgg ccggtgaagc gcgcgaccag atcgtcataa
     1861 cgcccgccgc ccgcgaccga accgaacacc accgtctcgc ccgcttcgtt ggtaacgggg
     1921 aaggtcaatt gcgcctcgaa caccgggccg gtgtaataat cgagaccgcg aataatcgtg
     1981 ggatcgat
//
LOCUS       DASWPU010000297         4451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_57468,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000297 DASWPU010000000
VERSION     DASWPU010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57468"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VGC27_06340"
     CDS             <1..717
                     /locus_tag="VGC27_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0282225.1"
                     /translation="IITLILLLPAVVTLLLQPDVGQTFLMLALWAALLFFSGLTLKWI
                     WVIGGGSLGLAGLAYFLFSHVRHRVLQYVMQFFDPGQAGYQTGLSLKAFAHGGLFGVG
                     PGAGTVKYRLPDAHSDFIYAVAGEEFGLVFCAVIALLFAVLTIRLLLRSAAAREPFAQ
                     LAGAGLAIVVAIQAFINMGVAVSLLPAKGMTLPFISYGGSSLFAVALTMGFALALIRH
                     RPQIAAREQPLFGLLKGARA"
     gene            714..1805
                     /gene="murG"
                     /locus_tag="VGC27_06345"
     CDS             714..1805
                     /gene="murG"
                     /locus_tag="VGC27_06345"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY0282226.1"
                     /translation="MTTIVLSAGGTGGHLFPAQALAGELVRRGRQVVVMTDSRGRGFT
                     DAFPGARIEIVPSAAFSDRSALGLVSAPFEIAAGILVSLWKLARIRPKVVIGFGGYPS
                     VPVMVAACLARYPTAILAPDAVLGRANRLVADYVRAIAANFPLVRFLPKDMSKVVYTG
                     NPLRPEVVALAGAPYDTPRPDGPLRLLVFGGSQGARVFRERVPPAIELLSAEIRSRLD
                     IVQQCRPEDLEAVRAVYAAMGVKATVAPFFRDLPERMAWAHLVVARSGAGTVSELAVI
                     GRPAILVPLPHALDDNQTPNADALASVGGGWRVAQKDLTPDKLAGMLAAAFDDPAALT
                     KCAAAARTLAKPDATLRLADLVERLEAEG"
     gene            1802..3232
                     /gene="murC"
                     /locus_tag="VGC27_06350"
     CDS             1802..3232
                     /gene="murC"
                     /locus_tag="VGC27_06350"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY0282227.1"
                     /translation="MIRPVQTRVPLDIGAIHFIGIGGIGMSGIAEIMHNLGYKVQGSD
                     SSDGTNVRRLRAMGIAVTVGHAADNLKDACVVVYSSAVKPGNVEFDAARVKGLPLVRR
                     AEMLAEIMRLKSCIAVAGTNGKTTTTTMVAALLDAGGLDPTVVNGGIINAYGTNARLG
                     AGEWVVVEADESDGTFLRLPAMVAVVTNADPDHLDYYGTFDKMRDAFQRFIENVPFYG
                     FAVLCIDHPEVQAMVGRIVDRRLVTYGMSPQADVRAVNVGFSEGISHYDVVFANRNKG
                     VETRLDDLLLPMPGEHNVQNSLAALAVARELGVPDETIRRAFGAFKGVNRRFTKVGEW
                     NGAAIIDDYAHNPFKIAAALKAARQAYAGPIVAVVQPHRFTRLRDTFEQFAKCLNDAD
                     MAVIAPVYPAGEQPIEGFDRDSYAEALRAHGHRNVATIEGEAGLAAAVAPFAKPGGAI
                     VCLGAGSITHWAHGLQAALEKLEGKA"
     gene            3232..4131
                     /gene="murB"
                     /locus_tag="VGC27_06355"
     CDS             3232..4131
                     /gene="murB"
                     /locus_tag="VGC27_06355"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY0282228.1"
                     /translation="MQNRPGDVLPPVRGSYTHGASLKDLVWFRAGGAAEILFRPADAD
                     DLASFLAARPRDMNLTVIGVGSNLLVRDGGIPGVVIRLPAAFGKVSVQGTRLRAGAAA
                     LDSAVSRAAADNGIAGLEFLRGIPGTVGGALKMNAGCYGREVKDIFVEATAVDSEGGK
                     HVLDAAAMGFAYRRSSVPEDFIFVEAVFEGTRDDPAAIRARMAEMMAQREAIQPVNSR
                     TGGSTFKNPPGQNAWQLIEEAGCRGLTHGDVQVSEKHCNFLINTGAATAADIEALGEE
                     VRARVKAKSGVELEWEIKRVGVP"
     gene            4128..>4451
                     /locus_tag="VGC27_06360"
     CDS             4128..>4451
                     /locus_tag="VGC27_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY0282229.1"
                     /translation="MTVKRVAVLLGGRSAEREISLVSGAGCAKALREEGFEVVEIDPQ
                     TSDLAAQINAAKPDVVFNALHGRWGEDGCVQGLLELLAIPYTHSGVLASALAMHKQRA
                     KDVFRA"
BASE COUNT          662 a         1497 c         1484 g          808 t
ORIGIN      
        1 atcatcacct tgatcttgct gctgccggcg gtggtcacgc tgctgctgca acccgatgtc
       61 ggccagacct tcctgatgct ggcgctgtgg gcggcgctgc tgttcttctc cggtctgacg
      121 ctgaaatgga tctgggtgat cggcggcggt tcgctggggc tggccgggct tgcctatttt
      181 ctcttttcgc atgtgcgcca ccgcgtgttg cagtacgtca tgcaattctt cgacccgggg
      241 caagccggtt atcagacggg cttgtcgctg aaggcgttcg cgcatggcgg actcttcggt
      301 gtcggtccgg gcgccggaac ggtcaaatac cgcctgcccg acgcccattc cgactttata
      361 tatgcggtcg ccggcgagga gttcggcctg gtgttttgcg ccgtcatcgc gctgctgttc
      421 gccgttctga ccatccggct gctgctgcgc tcggcggcgg cgcgcgaacc tttcgcgcaa
      481 ctcgcgggcg cgggcctcgc catcgtcgtc gcgatccagg ctttcatcaa catgggcgtc
      541 gccgtcagcc tgttgccggc gaaaggtatg acgctgccgt tcatttccta tggcggttcg
      601 tcgctgtttg ccgtggcgtt gaccatgggc tttgcgctgg cgctgatccg tcaccgcccg
      661 cagatcgcgg cgcgcgagca gcctttgttc ggcctcctaa agggggcgcg cgcatgacga
      721 cgatcgtgct ttcggcgggc ggcaccggcg ggcatctgtt tccggcgcag gcgctggcgg
      781 gcgaactggt ccgccgcggc aggcaggtcg tggtgatgac ggattcgcgt ggccgcggct
      841 tcaccgatgc cttccccggc gcgcgcatcg agatcgtgcc gtcggcggcc ttctccgacc
      901 gttcggcctt gggcctggtc agcgcgccgt tcgaaatcgc cgccggcatc ctcgtctcgc
      961 tatggaagct ggcacgcatc cgtcccaagg tggtgatcgg cttcggcggc tatcccagcg
     1021 taccggtaat ggttgcggcg tgcctggcgc gctatcccac cgccattctt gcgcccgatg
     1081 cggtgctcgg ccgcgccaat cgcctggtcg ccgactacgt gcgcgcgatc gccgccaatt
     1141 tcccgctggt gcgcttcttg ccgaaggata tgtcgaaggt cgtctatacc ggcaatcctc
     1201 tgcgccccga agtggtcgcc ttggccggag cgccttacga cacgcccagg ccggacggcc
     1261 cgttgcgcct gctcgttttc ggcggcagcc agggcgcgcg cgtgttccgc gagcgggtgc
     1321 cgcccgccat cgaactgttg tcggcggaga tcaggtcgcg gctcgacatc gttcagcaat
     1381 gccgccccga ggacttggag gcggtgcgcg ccgtctatgc cgcgatgggt gtgaaggcaa
     1441 cggtggcgcc gttcttccgc gatctgcccg agcgcatggc ctgggctcat ctggtggtcg
     1501 cgcgttccgg cgcgggcacg gtcagcgagc tggccgtgat cggccggccg gcgatcctgg
     1561 tgccactgcc gcacgcgctc gacgataacc agacgccgaa cgccgatgcg cttgcctccg
     1621 tcggcggcgg ctggcgcgtg gcgcagaagg acctcacgcc cgacaagctc gccggcatgc
     1681 tggcggccgc cttcgacgat cccgcggcac tgacgaaatg cgccgccgcc gcgcgcactc
     1741 tggccaaacc cgacgccacg ctgcgcctcg ccgatctggt ggaaagactg gaggccgagg
     1801 gatgatcagg cccgttcaga cccgcgttcc cctcgacatc ggggcgatcc atttcatcgg
     1861 catcggcggt atcggcatga gcggcatcgc cgagatcatg cacaatctgg gctacaaggt
     1921 tcagggttcg gattcctccg acggcaccaa tgtccggcgg ctgcgcgcca tgggcattgc
     1981 ggtcaccgtc ggccatgccg ccgacaatct caaggacgcc tgtgtcgtcg tctattcctc
     2041 ggcggtgaag ccgggcaacg tcgaattcga cgcggcgcgc gtcaaaggcc tgccgctggt
     2101 gcgccgggcc gaaatgctgg ccgagatcat gcggctgaaa tcctgcatcg ccgtggcggg
     2161 caccaacggc aagaccacga ccacgaccat ggttgcggcg ctgctcgatg cgggcgggct
     2221 cgatcccacc gtggtcaacg gcggcatcat caacgcctac ggcaccaacg cgcggctggg
     2281 cgcgggcgaa tgggtggtgg tggaggccga cgaaagcgac ggcaccttcc tgcgcctgcc
     2341 ggcgatggtc gccgtcgtca ccaacgccga ccccgatcat ctcgattatt acggcacctt
     2401 cgacaagatg cgcgacgcct tccagcgctt catcgaaaac gtgccgttct acggtttcgc
     2461 ggtgctgtgc atcgatcatc ccgaagtcca ggcgatggtg gggcgcatcg tcgatcgcag
     2521 gctcgtcacc tacggcatgt cgccgcaagc cgatgtgcgc gcggtcaatg tcggcttctc
     2581 cgagggcatc tcgcattacg acgtggtctt cgccaatcgc aacaagggcg tcgagacgcg
     2641 tcttgacgat ttgcttctgc cgatgccggg cgagcacaac gtgcagaatt ccctggccgc
     2701 gctcgccgtg gcgcgcgaac tgggcgtgcc cgacgaaacc atccgccgcg cgttcggcgc
     2761 ctttaagggc gtcaaccggc gcttcaccaa ggtcggcgag tggaacggcg ccgccattat
     2821 cgacgattac gcccacaacc cgttcaagat cgcggccgcc ttgaaggccg cgcgccaggc
     2881 ctatgccggc cccatcgtcg ccgtggtcca accgcatcgc ttcacccgcc tgcgcgacac
     2941 tttcgagcag ttcgccaaat gcctcaacga tgccgacatg gccgtgatcg ctccggttta
     3001 tccggcgggc gagcagccca tcgaaggctt cgaccgcgat tcctatgccg aggccttgcg
     3061 ggcgcacggc caccgcaatg tcgcgaccat cgaaggcgaa gccggtcttg ccgccgcggt
     3121 cgcgcctttt gccaaaccgg gcggcgccat cgtctgcctg ggcgccggtt cgatcaccca
     3181 ttgggcgcac ggcctgcaag cggcgctcga aaagctggag ggcaaagcgt gatgcaaaac
     3241 cgtcccggcg acgtgcttcc tcccgtgcgc ggcagctaca cccatggcgc gtcgctgaag
     3301 gatcttgtct ggttccgcgc cggcggtgcg gcggaaatat tgttccgtcc cgccgatgcc
     3361 gacgatctgg caagcttcct cgccgcgcgg ccgcgcgaca tgaacctcac ggtgatcggc
     3421 gtcggctcca atctgttggt gcgcgacggc ggcattcccg gcgtggtgat ccgcctgccg
     3481 gccgcgttcg gcaaagtgtc cgtgcaaggc acgcgcctgc gcgccggggc ggccgcgctc
     3541 gattccgccg tgtcccgcgc ggcggccgac aacggcatcg cgggcctgga gttcctgcgc
     3601 gggatccccg gcaccgtcgg cggcgcgctc aagatgaacg cgggctgcta cggccgcgag
     3661 gtcaaggaca ttttcgtcga ggccacggcc gtcgactccg agggcggcaa gcatgtgctg
     3721 gacgccgccg ccatgggctt cgcctatcgc agatcttccg tgccggagga tttcattttc
     3781 gtggaagcgg tgtttgaagg gaccagagac gatcccgccg ccattcgcgc ccgcatggcc
     3841 gagatgatgg cgcagcgtga agccatccag ccggtgaatt cgcgcaccgg cggctcgacc
     3901 ttcaagaatc cgcccggcca gaacgcctgg cagctcatcg aagaagcggg ctgccgcggc
     3961 ctgacgcatg gcgacgtgca ggtttcggaa aagcactgca acttcctcat caacacgggg
     4021 gctgcgaccg ccgccgacat cgaggcgctg ggcgaagagg tccgcgcgcg ggtgaaggca
     4081 aagtcgggcg tcgaactcga atgggaaatc aaacgggtgg gcgtgccatg acggtcaagc
     4141 gggttgcggt tctgctcggc gggcgttcgg cggaacgcga gatcagcctg gtttcgggcg
     4201 ccggctgcgc caaggcgctg cgcgaggaag gtttcgaggt cgtcgaaatc gatccgcaga
     4261 cttccgatct cgccgcccag atcaacgccg ccaagcccga cgtcgtgttc aacgccctgc
     4321 acggccgctg gggcgaggac ggctgcgtgc aagggctgct ggagcttctc gctattccct
     4381 atacccattc cggcgtgctc gcctccgcgc tggccatgca caagcagcgc gccaaggacg
     4441 tgttccgcgc c
//
LOCUS       DASWPU010000298         4482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_100694,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000298 DASWPU010000000
VERSION     DASWPU010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100694"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VGC27_06365"
     CDS             <1..406
                     /locus_tag="VGC27_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282230.1"
                     /translation="LLNIRKEHLHQHRHAGGFLRHGHEPAHEMEPGGDKELSRSRSLL
                     RAPLLIGIVHGTAGSAPFLALIPAAFMSPAKGILYVAIFSIGVLAGMLCFAVLLRSGQ
                     GWLNRRAHSLGYLCRGAFAAVAMSAGVFWIVG"
     gene            411..1799
                     /gene="nadD"
                     /locus_tag="VGC27_06370"
     CDS             411..1799
                     /gene="nadD"
                     /locus_tag="VGC27_06370"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY0282231.1"
                     /translation="MRLGLFGGKFNPVHLGHLVAAQTAAEALAFDRLVFVPVGRPMRK
                     DSPELADGTARLAMLRLAVGDNPLYDVSDVEIARPGKSMTIDTVRHFRAAYPDAEITF
                     LLGSDVIDRLHQWQEIDELRRLCRFAVLLRAGQDRPEVEPEIPRLRVPAIDISSTVLR
                     ARRLKHQAIDFLTPPPVADYIESHALYTPAALLQQVQAVLGEIGPRVVCCSGGVDSML
                     LATIAHRLAPADTVVAHAISPAVPDEATGRVRSWGNQEGWRLELVTSNEFTDERYLAN
                     PVNRCYFCKSHLYDTLKVIAAGMPRGAVMLSGANTDDLGEYRPGLTAAAEYNVRHPFV
                     ETGIDKAAIRAIAAHLELPFADLPAAPCLASRVYTGTRVTPARMRAIEEAERAIREAT
                     GVKVVRARVRENQMIVEMLESERERGSAAVLAEALRRAQSAEPGIAGIVLDPEPYRPG
                     RAFIGEHRDDQL"
     gene            1783..2445
                     /gene="larB"
                     /locus_tag="VGC27_06375"
     CDS             1783..2445
                     /gene="larB"
                     /locus_tag="VGC27_06375"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEY0282232.1"
                     /translation="MTTNYDYDRAERLGMPEAVLCESKDRESLDRLLDELHAKAGTPV
                     LFTRLTRPQFELIRPDLAALLDYDEVSGTAYLHGVRHGRPGRVAVVCAGTSDLPVARE
                     AVRTLTFMGIAQTLFCDVGVAGLHRLLDRIDEIRRHDIVIAVAGMDAALASVVGGLAG
                     CPVIGVPTSVGYGVAADGRTALNAMLTSCAQGVLVTNIDNGFGAACGAVRMLNVLSNR
                     SK"
     gene            2445..3176
                     /gene="larC"
                     /locus_tag="VGC27_06380"
     CDS             2445..3176
                     /gene="larC"
                     /locus_tag="VGC27_06380"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HEY0282233.1"
                     /translation="MSGLSVVVRTPSGLSGDMLLTGLSRLAAVSEAELDALTDAIGVP
                     ALANAVRIFPHAVGGVSGWQAVVDLTHEHHHRTYKAIVEIIKASALAAAAKELAANAF
                     AILAEAEARVHGITADAVHFHEVGALDSILDICLASALLAKIAPVNLYCSPLPICDGA
                     IHCEHGILASPAPAVQEMLAEVPVYGVDAEGETVTPTALALLKAAKAKFGKWPDCTVV
                     NQARVYGGKTFANLPNGALFFAVRE"
     gene            complement(3253..3491)
                     /locus_tag="VGC27_06385"
                     /pseudo
     CDS             complement(3253..3491)
                     /locus_tag="VGC27_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612145.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3829..>4482
                     /locus_tag="VGC27_06390"
     CDS             3829..>4482
                     /locus_tag="VGC27_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEY0282234.1"
                     /translation="MPDDGEVLRDRVVAAGGRAITDSALASFILMLAFLGRPADPAQL
                     LHMLGKGSAALGLDDLLVLAKRLDIKARRLKTSDAKLDYLPLPAIARLLDGSYLLLLQ
                     TSETEVLLFRPGDERPAQVNRSIFADEFSGELLLMTTRDRIAGAARSFDVSWFIPALV
                     KYRQLLRDVLFASFFIQLLGLASPVFFQVVIDKVLVHKGMTTLEILAIGLFFVSIFEI
                     "
BASE COUNT          745 a         1437 c         1445 g          855 t
ORIGIN      
        1 gctcctcaat atccggaaag aacatctcca tcagcatagg cacgccggcg gttttttgcg
       61 gcatggccac gaaccggcgc acgagatgga gccgggcggc gacaaggagc tttctcgctc
      121 gcgttcgctg ctgcgcgccc cgttacttat cggcatcgtc cacggcaccg ccggcagcgc
      181 gccgttcctg gcgctgatcc ccgccgcctt catgagcccg gcgaagggga ttctctacgt
      241 cgccattttt tcgatcggcg ttctggcggg gatgctttgc ttcgccgtgc tgctgcggag
      301 cggccagggt tggctcaatc ggcgcgcgca ctcgctcggc tacctctgcc ggggcgcgtt
      361 cgcggcggtc gccatgagcg ccggcgtttt ctggatcgtc ggatagtatc atgcggctcg
      421 ggctgttcgg cggaaagttc aatcccgtgc atctcgggca cctggtcgcg gcacagacgg
      481 cggccgaggc tttggcgttc gaccggcttg tcttcgtccc ggtcggccgg ccgatgcgga
      541 aggacagccc agagttggca gacggtacgg cacggctcgc catgctgcgc ctggccgttg
      601 gcgacaatcc gctctacgac gtcagcgacg tcgagatcgc acgcccgggc aagtcgatga
      661 ccatcgatac ggttcgccat tttcgcgcgg cgtatccgga tgcggaaatc accttcctgc
      721 tcggttcgga cgtgatcgat cgcctccacc agtggcagga gatcgacgaa ttgcgccgcc
      781 tgtgccggtt cgctgtgttg ctgcgcgcag gccaggaccg cccagaggtc gagccggaga
      841 ttccccgctt gcgggttccc gccatcgaca tttcctcgac cgttctccgc gcgcggcgcc
      901 tcaagcatca ggccatcgac ttcctgacgc cgccgccggt cgccgattac atcgaaagcc
      961 acgcgctcta cacccctgcc gccctgttgc agcaggtgca ggcggtcttg ggtgaaatcg
     1021 ggccgcgggt ggtctgctgc tccggcggcg tcgattccat gctgttggcc actatcgctc
     1081 accgcttggc gccggccgat acagtggtcg cccacgcgat cagcccggcg gtgccggacg
     1141 aagccaccgg gcgcgtacgg tcgtggggca atcaggaagg ctggaggctg gaactggtga
     1201 ccagcaacga gttcaccgac gagcgctatc tcgccaatcc ggtgaatcgc tgctatttct
     1261 gcaagtcgca cctttacgat acgctcaagg tgatcgccgc cggcatgccg aggggcgccg
     1321 tcatgctctc gggcgccaac accgacgatc tcggcgagta tcgcccgggg ctgaccgcgg
     1381 cggcggaata caatgtccgc catcccttcg tcgaaaccgg catcgacaag gccgccatcc
     1441 gcgccattgc ggcgcatctc gaactgccgt tcgccgatct gccggcggcg ccgtgcctgg
     1501 ccagccgggt ctataccggc acgcgcgtca cccccgcgcg gatgcgcgcg atcgaggaag
     1561 cggaacgcgc catccgcgag gcgaccggcg tcaaggtggt gcgggcgcgc gtgcgggaaa
     1621 accagatgat cgtggagatg ctggaaagcg agcgggaacg cggcagcgcg gcggtcctcg
     1681 ccgaggcgct gcgccgcgcg caaagtgccg agccggggat cgccggcatc gtcctcgatc
     1741 cggaacccta ccggcccggc cgggccttta tcggtgagca ccgtgacgac caactatgac
     1801 tatgatcgcg ccgagcggct cggcatgccg gaagcggtgc tgtgcgagag caaggatcgc
     1861 gaaagcctcg accgtctgct ggacgaactg cacgccaagg ccggcacccc ggttctgttc
     1921 acccggctca cccggccgca gttcgaattg atcaggccgg atctggcggc tttgctcgat
     1981 tacgacgaag tctccggcac ggcttatctg cacggcgtcc ggcacgggag gcccgggcgg
     2041 gtcgccgtcg tctgcgccgg aacctccgat ctgccggtgg cgcgtgaggc ggtgcggacc
     2101 ctgacgttca tggggatcgc gcagacgctg ttctgcgacg tcggcgttgc cgggcttcat
     2161 cgcctgctcg accggatcga cgagatccgc cgccacgata tcgtcatcgc cgtggcgggc
     2221 atggatgccg cgctggcctc ggtggtgggg ggactggccg gctgtccggt catcggggtt
     2281 ccgacttccg tcggttacgg tgtcgcggcc gacggacgga ccgccctgaa cgcgatgctg
     2341 accagttgcg cccagggtgt gctggtgacg aacatcgaca acggcttcgg agccgcctgc
     2401 ggcgccgtgc gcatgctgaa cgtcctctcg aacaggagca agtgatgagc ggtctttcgg
     2461 tcgtggtgcg tacgccttcg ggattgagcg gcgacatgct gctgaccggg ctttcccgct
     2521 tggccgcggt atcggaggcg gagttggacg cgctgaccga cgcgattggc gtgcccgctt
     2581 tggccaatgc cgtccgtatc tttccccatg cggtcggcgg cgtgtcgggc tggcaggctg
     2641 tagtcgatct gacgcacgaa catcatcacc gcacctacaa ggcgatcgtc gaaatcatca
     2701 aggccagcgc cctggcagcg gccgcgaagg agctggcggc caacgccttt gccatcctcg
     2761 ccgaggccga ggcccgcgtg cacggcatca ccgccgacgc ggtgcatttc cacgaggtgg
     2821 gggcgctgga cagcattctt gacatctgtc tggcctcggc gctgctggcg aagatcgcgc
     2881 ccgtcaacct ctattgcagc ccgctgccga tctgcgacgg tgcgatccac tgcgaacacg
     2941 gcatcctcgc cagtcccgcc ccggcggtgc aggagatgct ggctgaggtg cctgtctatg
     3001 gggtcgatgc cgagggcgaa acggtcaccc cgaccgcgct ggcgttgctg aaggcggcca
     3061 aggcgaaatt cggcaagtgg cccgattgca cggtggtcaa ccaggcccgg gtctatggcg
     3121 gcaagacctt cgccaatctg ccgaacggcg cgctgttttt tgccgtacgc gaatagacga
     3181 cggcactagc gtccacaatg tgcttaccga ccgtgtggcg caggccgtga taggtcaggt
     3241 acttcccgac tttatgcgat cccggacgcc tgccttgtgc catcggttga agcggttcac
     3301 gcaagtcgtg tgcggcccat atcgatccgg aatatccgcc catggcgccc tatgcacagc
     3361 cgccagaaga tcccgttcag aacctgccgg tcgtccaccc ctacaaccct gcgaatcttg
     3421 ttcggcagca aaggtgcaat caccgaccac tcgaagtccg tcaaatcgaa gcgagccgtc
     3481 caaacctcca acgttggaag tttgaatcac tgttcgccaa aaacggaatc ctctttatgg
     3541 gcatgtcacc tagagcgcaa tccggcaaag tgtgaagcgg tttgccgtca gattgcgcga
     3601 aaatcaagga atctaaagct tgatccgatt caattaaatc ggatcaagct ctagtccgcc
     3661 acggcaccta aagcgtgccg ccagccatca cgcgtcagaa acttgaatcg gccgcgcggc
     3721 gatatatccc ttgcggccaa tgccacaata ggttgaacta gcagtcaaaa gcccacgaag
     3781 ccggtctttg aacacattgg agacggcacc cgggctgtgg gggggcacgt gccggatgac
     3841 ggcgaagtct tgcgcgaccg tgtagttgcg gcggggggcc gcgcgatcac ggattcggcg
     3901 ctggcttcgt tcattttgat gcttgcgttt ctgggacgcc ccgcggaccc ggcacaactt
     3961 cttcatatgc tcggaaaagg gtcggcagca ctcggtcttg acgacctttt ggttctcgcc
     4021 aagaggctgg atatcaaggc tcgccgcctg aagacgagcg acgcgaagct cgactatctt
     4081 ccgttgcccg cgatcgcccg gctgttggac ggctcgtatc tgctgctgct tcagacatcg
     4141 gagaccgagg tcctgctgtt ccggccgggc gacgaacgcc cggcgcaggt caaccgttcg
     4201 atttttgcgg acgaattctc cggcgaactc ctgttgatga cgacgcgcga ccgcatcgcc
     4261 ggtgcggccc ggtcgttcga cgtgagttgg ttcatccccg cgctggtcaa atatcgtcaa
     4321 ttgctgcgcg atgtgctgtt cgcgtcgttt ttcatccagt tgctcgggtt ggcgtcgccg
     4381 gtgttcttcc aggtcgtcat cgacaaggtg ctggtgcaca aaggtatgac gaccctcgag
     4441 atattggcga tcggcctgtt cttcgtctcg atcttcgaga tc
//
LOCUS       DASWPU010000299         6407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_51552,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000299 DASWPU010000000
VERSION     DASWPU010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51552"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2456
                     /locus_tag="VGC27_06395"
     CDS             <1..2456
                     /locus_tag="VGC27_06395"
                     /inference="COORDINATES: protein motif:HMM:NF009415.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEY0282235.1"
                     /translation="DRYPMFKGLSKDWPSGNFIHPYVRPAEERNHDFELVTTGYLGYR
                     ALGYSMREVDLFVWLEVLRDKQCDDRPCELGQMLKGKKKGGCPVVIHIPDMLNLMGQG
                     KFKEAMKLIQASNPLPNVTGRVCPQENQCQGVCTMTGRPIEIGQLEWFLPERQRLAEK
                     ANGADENGWKDYVSPWDKAEKPPIAVVGSGPAGLINAYLLSVEGFPVTVFEAFHDLGG
                     VLRYGIPEFRLPNALIDDVVGKIDRLGGKFVKNFVVGKTATLQDLKDAGFWKIFVGTG
                     AGLPRFMNIPGEHLNGVMSANEFLTRVNLMQARMDDFETPLPIVKDKNVIVIGGGNTA
                     MDAARTSKRLGANITIVYRRTKKEMPARVEELEHALEEGVKLSVLRGPTEFVSGGDHH
                     VCHAILDVMELGKPDASGRRTPVPTGKTETIPVDLVIMALGNDSNPIIKDSEPRIKTS
                     KWGTLLVENGAETTLEGVYSGGDATRGGSTAINAAGDGKAAADQIAKDIPFSAAEIKQ
                     LVAKAGAYTEKGQTAYTVVKRTDLATGIVELTVKAPMVAKSAKAGQFVRLLADAKGEL
                     IPLTLADWDAKAGTIDLVVQGVGTSSILMNQRKVGESFTGIAGPLGQPSKLHRYEANQ
                     TVVFAAGGVGLPPVYPIMRAHLELGNHVTLIAGYRTKGALFWTAQGERVDKLQKKFGK
                     QLDVIYCTNDGTFGVKGFVTTPLQEMMDAGKASKGRAIAEVIAIGPPLMMRAVSELTR
                     KYAVPTVVSLNSIMVDATGMCGACMVPVTIDGKMVRKHACIDGPELDAHIIDWDKFLP
                     RFDQFKPQEKVSREKHGLA"
     gene            2917..5562
                     /locus_tag="VGC27_06400"
     CDS             2917..5562
                     /locus_tag="VGC27_06400"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0282236.1"
                     /translation="MAFLIVLVFSFSAAAAPAPGRDIVRAKLSNGLRVVVVRNTLAPV
                     VSTSVNYLVGSNEAPAGFPGLAHALEHMMFRGSPGLSADQLANIGSVMGGQFNANTRE
                     SLTQYLYTVPAEDLDVALRIEALRMQAVSNDEKAWDKERGAIKQEVAQNLSLPAYKLY
                     QKLRAELFAGTPYEHDALGTQRSFDNTAAAMLKDFHDKWYAPNNAILVIVGDVDPKQA
                     LAKVKRLFGPLKAKKLPARPEFKFKPVRPASLTIDTDSPYETQVIAMRLPGLRSRDFP
                     ALEVLADVLASRRFDLYGMVAGGKAVAAGFSLDILPKAGIGYALVSFSPGGDAKAIEA
                     EMRAILTKVAKEGVPAELVEAAKRQERLDDAQQKNSIAGLASVWSDAIALHGLRSPDR
                     NLKRIQKVTVADVNRVARKYLRLDSAISALLLPHGSGKPIASGGGFGGQETIALGEAN
                     ATSLPDWAETAFSRLSLPASTKQPAVTTLANGLTLIVQPENVSDTVAVYGHIRNRPQT
                     EAPPGKDGVDLLLNPLLSFGSERLDRLAFQQALDEIGASERAGTDFSVEVLAQDFDRG
                     VELLADNQLRPALPEEAMALIKPQIAKAIASRNASPAFLAQRAMREALFPPSDPSLRD
                     ATEQSVSALTLDDVRSYYRTVFRPDLTTIVVIGNITPEKARAGIEKYFGAWTAAGPKP
                     ETDLPAVPPNRAASLAVPDASRVQDKVMLAHTLSLKRSDPDYYALSLGNAVLGGGFYS
                     TRLSIGLRKNTGLVYSVGTDLNIGRVRGLYNVEYACDPQNVDKAAAFIAQEIKVMQTK
                     PVEAGELMRAKALLLRQMPLSESSITGIAGGFLSRQELDLPLDEPAVAAKRYIELEPA
                     DIEAAFAKWMRPDDLVRVTQGPPPQ"
     gene            complement(5623..>6407)
                     /locus_tag="VGC27_06405"
     CDS             complement(5623..>6407)
                     /locus_tag="VGC27_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0282237.1"
                     /translation="KLAKSGKHILLEKPLEITQQRSQALVAAGAAAGVKIAVCLQHRF
                     RAAAERLGALLAEGSLGKIVACSTRIPLWRPQAYYDEPGRGTKARDGGGVLISQGIHT
                     LDLMLSLAGPVDEVCGYAVTSAVHRMETEDLACAAMRFANGAIGTIEASTAAYSGGEA
                     LIDFTGTKGSAVLAGTALSVQWHDGRREDMAAESFGGGGADPMAFPHHHHLAVWRDFL
                     DAIDGNKEPRVSAKEALKVHKLIDALLAAGASDRKIKVANSE"
BASE COUNT         1204 a         2072 c         2026 g         1105 t
ORIGIN      
        1 gcgaccgcta cccgatgttc aaagggcttt cgaaggactg gccgagcggg aacttcatcc
       61 acccctacgt tcgccccgcg gaagagcgca atcacgactt cgaactcgtc accaccggct
      121 atctcggcta tcgcgccctc ggttactcga tgcgcgaggt cgacctgttc gtctggctcg
      181 aagtgctgcg cgacaagcag tgcgacgacc ggccttgcga actgggccaa atgctgaaag
      241 gcaagaagaa gggcggctgc ccggtggtga tccacatccc ggacatgctc aacctgatgg
      301 gccagggcaa gttcaaggag gcgatgaagc tgatccaggc gagcaacccg ctgccgaacg
      361 tcaccggccg cgtctgcccg caggaaaacc agtgccaggg cgtctgcacc atgaccggcc
      421 ggccgatcga aatcggccag ctcgaatggt tcctgcccga acgccaaagg ctggcggaaa
      481 aggcgaacgg agccgacgag aacggctgga aggattatgt cagcccgtgg gataaagccg
      541 agaagccgcc gattgcggtg gtcgggtccg gtcccgccgg actgatcaat gcctatctgt
      601 tgtcggtcga aggcttcccg gtcacggtgt tcgaggcgtt ccacgacctc ggcggcgtgc
      661 tgcgttacgg cattcccgaa ttccgtctgc ccaacgcgct gatcgacgac gtggtcggca
      721 agatcgaccg tctcggcggc aagttcgtga agaacttcgt cgtcggcaag accgcgaccc
      781 tgcaagacct caaggacgcc ggcttctgga agattttcgt cggcaccggc gcgggactgc
      841 cgcgcttcat gaacatcccc ggcgagcatc tcaacggcgt gatgagcgcc aacgagttcc
      901 tcacccgcgt gaatctgatg caggcgagga tggacgattt cgagaccccg cttccgatcg
      961 tcaaggacaa gaacgtcatc gtcatcggcg gcggcaacac cgcgatggac gcggcgcgca
     1021 cctccaagcg gctgggcgcc aacatcacca tcgtctatcg ccgcaccaag aaggaaatgc
     1081 cggcgcgcgt cgaggaactc gagcatgcgc tggaggaagg cgtgaagctt tcggtcctgc
     1141 gcggccccac cgagttcgtc agcggcggcg atcaccatgt ctgccacgcc attctggatg
     1201 tcatggaact tggcaaaccg gatgcttcgg ggcggcgtac gccggtgccc acgggcaaga
     1261 ccgagaccat cccggtcgat ctggtgatca tggcgctggg caacgactcc aacccgatca
     1321 tcaaggactc cgaaccccgc attaaaacct cgaaatgggg cacgctcctg gtcgagaacg
     1381 gcgccgagac gacgctcgag ggcgtttact cgggcggcga cgcgacgcgc ggcggttcga
     1441 cggccatcaa tgcggcgggc gacggcaagg cggcggcgga tcagatcgcc aaggacattc
     1501 cgttcagcgc ggccgagatc aagcagcttg tcgccaaggc cggcgcctat accgagaaag
     1561 gccagacggc ctacacggtc gtcaagcgca ccgatctggc gaccggcatc gtcgaactga
     1621 cggtcaaggc tccgatggtc gccaaatcgg cgaaggccgg gcaattcgtg cgcctgctgg
     1681 cggacgccaa gggcgaactc atcccgctca ccctggccga ctgggacgcc aaggcgggca
     1741 ccatcgactt ggtcgtccag ggcgtcggca cctcaagcat cctgatgaac cagcgcaagg
     1801 tcggcgaatc cttcaccggc atcgccggac cgctcggcca gccgtcgaag ctgcaccgct
     1861 acgaagccaa ccagacggtc gtgttcgcgg cgggcggcgt cggtctgccg ccggtttatc
     1921 cgatcatgcg cgcgcatctg gagctgggaa accatgttac cctgatcgcc ggctatcgca
     1981 ccaagggcgc gttgttctgg acggcgcaag gcgagcgcgt cgacaagctg caaaagaaat
     2041 tcggcaagca gctcgacgtg atctactgca ccaacgacgg caccttcggc gtcaagggct
     2101 tcgtcaccac gcccttgcag gagatgatgg acgcgggcaa ggccagcaag ggccgcgcca
     2161 tcgccgaagt catcgccatc gggccgccgc tgatgatgcg ggcggtcagc gaactgacca
     2221 ggaaatacgc ggttccgacc gtcgtcagcc tgaactcgat catggtggac gccaccggca
     2281 tgtgcggcgc ctgcatggtc ccggtcacga tcgacggcaa gatggtccgc aagcacgcct
     2341 gcatcgacgg tcctgaactc gacgcccaca tcatcgactg ggacaagttc ctgccccgct
     2401 tcgaccagtt caaaccgcag gaaaaggtga gccgcgaaaa gcacgggctt gcttgagttt
     2461 cgcagcctcg ggggaaggga cacactcttc ccctaaggcc cgatctcaat aagaaaacct
     2521 tcacccaaac ttgtcatggc ccgcatcctt cgactcgtcg ccctcatcct gaggagcgag
     2581 cgtagcgagt gtctcgaagg acgagggcga cagctcagga taaggcgcgg gccatccggg
     2641 tgatcgcagg tttcatcgga tcgctcccgc cgcccttcga caggctcagg gtgagggggg
     2701 ctaatccttc atgctgagcc tgtcgaagca tgggtgtggc gcgaacccat gtggatggat
     2761 catgccctaa atttcttgtc atcgaaccgg ggccgccgcc gcttgggcgg ctcacggaga
     2821 ttttcgcccg gtcccggcga ttctgcccta taagaacggc taaaccgcac aagggaagat
     2881 ctcatgggtc gcctcgcgag cagccgtgtt ccttttatgg ccttcctgat tgtcctggta
     2941 ttttcgtttt ccgccgccgc cgctccggcg cccgggcggg atatcgtccg cgccaagctt
     3001 tcaaacggcc tgcgcgtggt cgtcgtccgc aatacgctgg cgccggtggt ttcgacatcc
     3061 gtcaactatc tcgtcggttc gaacgaggcg ccggcgggct ttcccggcct ggcccatgcg
     3121 ctggagcata tgatgttccg cggcagcccc ggcctttccg ccgatcagct tgccaacatc
     3181 ggcagcgtga tgggcgggca gttcaacgcc aatacgcgcg agagcctcac ccagtatctg
     3241 tacacggttc ccgccgagga cctcgacgtc gcccttcgta tcgaagcgct gaggatgcag
     3301 gcggtctcga acgacgaaaa ggcgtgggac aaggaacgcg gcgcgatcaa gcaggaagtg
     3361 gcgcagaatc tctccctgcc cgcctataag ctttaccaaa agctgcgggc ggaactgttc
     3421 gccggcacgc cttacgaaca tgatgcgctc ggcacgcagc ggtctttcga caacaccgcc
     3481 gccgccatgc tcaaggattt ccacgacaaa tggtatgcgc cgaacaatgc gatcctggtg
     3541 atcgtcggcg atgtggaccc caagcaggcg ttggcgaagg tcaagcgcct gttcggcccg
     3601 ctcaaggcca aaaagctgcc cgcccgcccc gaattcaagt tcaagcccgt gcggcccgcc
     3661 tcgctgacca tcgacaccga cagtccttac gaaacccagg tcatcgcgat gcgcctgccg
     3721 ggattgcgca gccgggattt tcccgcgctc gaagtgctgg ccgacgtgct ggcgagccgc
     3781 cggttcgacc tttacggaat ggttgcgggc ggcaaggcgg tggcggccgg tttttcgctc
     3841 gatattctgc ccaaggccgg catcggctat gcgctggttt cgttttcgcc gggcggcgac
     3901 gccaaggcga tcgaagcgga gatgcgcgcg attctgacga aggtcgccaa ggaaggcgtg
     3961 ccggccgagc tggtggaagc ggccaagcgg caggaacggc ttgatgacgc gcagcagaaa
     4021 aactccatcg ccggactggc ttcggtgtgg tccgatgcga ttgcccttca cgggcttcgt
     4081 tctcccgatc ggaatctcaa acggattcag aaggtgacgg tcgctgacgt aaaccgcgtg
     4141 gcgcgaaaat atctccggct ggatagcgcc atttccgcgc tgctgctgcc gcatggctcg
     4201 ggcaagccga ttgcttccgg cggcggcttc ggcggccagg agaccatcgc gctgggtgaa
     4261 gcaaatgcca cgtccttgcc cgattgggcc gagaccgctt tcagccgcct ttccttgccc
     4321 gcctcgacga agcagcccgc cgtcaccact cttgccaacg gcctcactct gatcgtgcag
     4381 ccggaaaacg tcagcgacac ggtggcggtc tacggccata tccgcaaccg gcctcagacc
     4441 gaggcgccgc cgggcaagga cggcgtggac ctgcttctca atcccctctt gtccttcggc
     4501 agcgagcggc tcgaccgcct cgccttccag caggcgctgg atgagatcgg cgccagcgaa
     4561 cgcgccggaa ccgatttttc ggtcgaggtt ctggcgcagg atttcgatcg cggcgtcgag
     4621 ttgctggccg acaaccagct ccgtcccgcg ttgcccgaag aggccatggc tctgatcaag
     4681 ccgcagatcg ccaaggcgat cgccagcagg aacgcaagcc ccgcctttct ggcgcagcgc
     4741 gccatgcgcg aggcgctgtt cccgccgtcc gatccgagcc tgcgcgacgc gacggagcaa
     4801 agcgtgagcg cgctgacgct ggacgatgtg cgctcctact atcgcaccgt gttccgcccc
     4861 gacctcacca cgatcgtggt gatcggcaac atcacgccgg aaaaggcgcg cgcgggcatc
     4921 gagaaatatt tcggcgcctg gacggccgcg gggccgaagc cggaaaccga cctgccggcg
     4981 gttccgccca accgcgccgc ctccctggcg gtgccggatg ccagccgcgt tcaggacaag
     5041 gtcatgctcg cccatacgct tagcctcaaa cgttccgatc ccgactatta cgcgctctcg
     5101 ctcggcaacg cggtgctggg cggaggcttc tattcgacca ggctcagcat cggcctcaga
     5161 aagaatacgg gtctggttta ttcggtcggc accgatctga acatcggcag agtccgcggc
     5221 ctctataacg tggaatatgc ctgcgatccg cagaacgtcg acaaggcggc cgccttcatc
     5281 gcgcaggaaa tcaaggtcat gcagaccaag ccggtggagg ctggtgagct gatgcgcgcc
     5341 aaggcgctgt tgctgcgcca gatgcccttg agcgagtcga gcatcaccgg aatcgccggc
     5401 gggtttctga gccggcagga actggatttg ccgctcgacg aaccggccgt ggctgccaag
     5461 cgctatatcg aactggagcc tgccgacatc gaggccgcct tcgccaagtg gatgcgcccc
     5521 gacgatctgg tgcgcgtcac ccaaggcccg ccgccgcaat aatttctgcc cccgcgaagc
     5581 gggggaagtg ccgaacgagc gaagcgagag aggcgaaggg ggctattcgc tattcgctac
     5641 cttgatcttg cggtccgagg ctcccgccgc gagcaacgca tcgatcagtt tgtggacctt
     5701 gagcgcttcc tttgccgaaa cgcgcggttc tttgttgccg tcgatggcgt ccagaaaatc
     5761 gcgccacacc gccagatggt gatggtgcgg gaaggccatg ggatcggcgc cgccgccgcc
     5821 gaacgattcc gccgccatgt cctcgcgtct gccgtcgtgc cactgcaccg aaagcgcggt
     5881 gccggcgagg acggccgacc ccttggttcc ggtaaaatcg atcaaggctt cgccgccgga
     5941 ataggctgcc gtgctggctt cgatagtccc gatggcgccg ttggcgaaac gcatggcggc
     6001 gcaggcgagg tcttcggttt ccatgcgatg cacggcgctg gtgacggcat agccgcagac
     6061 ctcatccacc ggcccggcga ggctcagcat caggtcgagc gtatggatgc cctgcgagat
     6121 cagcacgccg ccgccgtcgc gcgccttggt gccgcgtccg ggttcgtcgt aataggcttg
     6181 cggccgccac aacggaatcc tggtcgagca ggccacgatc ttgccaaggc ttccttcggc
     6241 cagaagggcg ccaagccgtt cggccgccgc cctgaaacgg tgttgcaggc agacggcgat
     6301 cttgacgccc gccgcggccc cggccgcgac caatgcctgg gatcgttgtt gggtgatttc
     6361 gagcggcttt tcgagcagga tgtgcttgcc gcttttggcc aattttt
//
LOCUS       DASWPU010000300         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_64011,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000300 DASWPU010000000
VERSION     DASWPU010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64011"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..760)
                     /locus_tag="VGC27_06410"
     CDS             complement(38..760)
                     /locus_tag="VGC27_06410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0282238.1"
                     /translation="MKVDRTGSVSGVRAVGAAAYAKQTRAVAAAEAPAPVSATASVLG
                     IPEAEFTPRVRDAIMTLMGEVDELRRELQRTRDRLEEAEKTADQDHLLPLLNRRAFVR
                     ELTRYISFTGRYGTPATLIYFDLDGFKTVNDTHGHAGGDAVLSHFAETLLSHVRDSDI
                     VGRLGGDEFGVLLTHANQEQGLKKADQLAENLRAAPTLWGGKSIPTNFSYGAFELKPG
                     DTADLAITKADEAMYAHKRTSR"
     gene            946..1434
                     /gene="purE"
                     /locus_tag="VGC27_06415"
     CDS             946..1434
                     /gene="purE"
                     /locus_tag="VGC27_06415"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY0282239.1"
                     /translation="MTTPLVGLIMGSQSDWPCLRSAADLLEALGVPYESKIVSAHRTP
                     DRAVAYAKTAAERGLKVIIAAAGGAAHLAGVTAAMTILPVLGVPMESKALKGLDSLLS
                     TVQMPGGVPVATFAIGEAGAKNAALHAVSILALADAALAARLAEWRRKQTDAVAEAPS
                     GK"
     gene            complement(1446..1916)
                     /locus_tag="VGC27_06420"
     CDS             complement(1446..1916)
                     /locus_tag="VGC27_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0282240.1"
                     /translation="MEQLDRRDLFAALTAFGALGALGALAGTARAQQMTTAELSVSKV
                     FTFDSLLETKLPNGGEMRRVLSGTLPTGEFIEIHETVLPAGKMPHPPHKHPNSEVLFI
                     QSGKLEYIDVDKPIRVGPGDVVFSASNVIHGLSNIGDTPATYIVVSVSKQLPEG"
     gene            complement(1940..2308)
                     /locus_tag="VGC27_06425"
     CDS             complement(1940..2308)
                     /locus_tag="VGC27_06425"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0282241.1"
                     /translation="MASDALSQSKVFTFDEMPVRKMANGGVSRDVVEGVVATGETVAI
                     HESVQPAGAPPNPAHRIEHSEFILVQDGTLEFRHDDKVERAGPGSVIYVAHGTMHGVR
                     NIGDGPAKYTVISIGGDVKK"
     gene            complement(2429..3076)
                     /locus_tag="VGC27_06430"
     CDS             complement(2429..3076)
                     /locus_tag="VGC27_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HEY0282242.1"
                     /translation="MTEKPVAKDDAALKGAVVEAALAHIPFDGFTDRLMNRAAKEAGA
                     SKADMLRLFPDGALGLLAAASESADAEMEERLGKLDLAAMPVRGRIAAAVKTRLALLK
                     PHKEAARRAVAHLSLPPNVALAAELVYRTVDAMWRAAGDVSTDFNFYTKRGILAGVYS
                     ATLLRWLADDSEDESATDAFLAARIENVMQFEKFKAQAKSTLARLGELAAAPNKA"
     gene            complement(3079..3630)
                     /gene="def"
                     /locus_tag="VGC27_06435"
     CDS             complement(3079..3630)
                     /gene="def"
                     /locus_tag="VGC27_06435"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0282243.1"
                     /translation="MAILKIARMGHPVLARRADPVADPTAPTIRRLVADMLETMIDAD
                     GAGLAAPQVHVPLRVVVFQAPGERTEEGLSEAERFDRTAPLTVLINPEIEILGPETED
                     GWEGCLSVPGLRGFVERPAHIRYRGVDHEGHSIERTAKGFHARVVQHECDHLNGILYP
                     QRMSDLTKLIFESEARYWVKGGE"
BASE COUNT          684 a         1254 c         1212 g          742 t
ORIGIN      
        1 agggtgaaag gaacaattca accggattga acatgcatta gcgcgacgtg cgcttgtgcg
       61 cgtacatcgc ttcgtcggcc ttggtgatgg cgagatcggc cgtgtcgccg ggtttcaatt
      121 caaaggcgcc gtaagaaaaa ttcgtcggaa tcgatttgcc gccccacaag gtgggcgccg
      181 cgcgcaggtt ttcggcgagt tgatccgctt tcttcaggcc ctgttcctga ttggcatgtg
      241 tcagaagcac gccgaattcg tcgccgccga gccgcccgac gatgtcgctg tcgcgcacat
      301 gggaaagcaa cgtctcggcg aagtggctga gcaccgcatc gccgccggca tggccgtggg
      361 tgtcgttcac cgtcttgaac ccgtcgaggt cgaaatagat gagcgtcgcg ggcgtcccgt
      421 agcggccggt gaaggagatg tagcgcgtca gttcgcgcac gaaggcgcgc cggttgagca
      481 gcggcagcag atgatcctgg tcggcggttt tttccgcttc ctcgagacgg tcgcgggtcc
      541 gttgcagctc gcggcgcagc tcgtcgactt cgcccatcag cgtcatgatg gcgtcgcgca
      601 cgcgcggcgt gaactcggcc tcggggatgc ccaacacgct ggccgtggcg ctgacggggg
      661 caggagcctc ggcggcggcg acggcccggg tctgcttggc ataagcagcc gcccccaccg
      721 cacgcacccc gctgacagag cccgttctgt cgactttcat gcgcttcgac cccttcgcct
      781 tgcggcctga tgaaacaacc taataacggc tcgttaataa ctcgtttatg atgacgaaca
      841 aaaggttaac tgttccaact gcccgctttc cttggatttt ttcgcctcta taatgcgccg
      901 cttccttcac cgccgattcg cggcccaacg ccaggacaag gaagcatgac caccccactc
      961 gtcggcctca tcatgggtag ccagtcggac tggccgtgcc tgcgcagcgc cgcggacctg
     1021 ctggaggcgc tgggtgtgcc gtatgaaagc aagatcgtct ctgcccaccg cacgcccgac
     1081 cgcgccgtcg cttatgccaa gacggcggcg gagcgcggct tgaaggtgat catcgcggcg
     1141 gccggaggcg cggcacatct ggcgggggtg acggccgcga tgacgattct gccggtgctc
     1201 ggcgtgccca tggaatccaa ggcgctcaag ggcctcgaca gccttctgtc caccgttcag
     1261 atgcccggcg gcgtgccggt cgcgaccttc gccatcggcg aggcgggcgc caagaacgcg
     1321 gcgctgcacg ccgtctccat cctggcgctc gccgacgcgg cgctggccgc gcggctggcg
     1381 gagtggcgcc gcaagcagac cgatgccgtc gccgaagcgc cctccggcaa atagaccaag
     1441 cgcgatcagc cctccggcaa ctgcttgctg accgaaacga cgatgtaggt cgccggcgta
     1501 tcgccgatat tcgacaagcc gtggatcacg ttggaggcgg agaacaccac atcgcccggc
     1561 ccgacgcgga tcggtttgtc gacgtcgata tactccagct tgcccgactg aatgaacagc
     1621 acttcggaat tgggatgctt gtgcggggga tggggcatct tgccggcggg cagcacggtt
     1681 tcgtggatct cgatgaactc gccggtcggc agcgtgccgg acagcacgcg gcgcatttca
     1741 ccgccattgg gcagtttggt ctccagcagc gagtcaaagg taaacacttt cgagaccgaa
     1801 agttccgccg tcgtcatttg ctgcgcccgc gccgtgcccg ccagcgcgcc cagcgcgccc
     1861 agcgcgccga aggccgtgag cgccgcgaac aaatcccgcc gatccaactg ttccatgatc
     1921 gcaatcctcc gcttgttttt tatttcttca catcgccgcc gatggaaatc accgtgtact
     1981 tggccggacc gtcgccgatg ttccgcacgc cgtgcatcgt gccgtgagcg acatagatta
     2041 cgctgcccgg cccggcccgt tcgactttgt cgtcgtgcct gaactcaagc gttccgtcct
     2101 gcaccaggat gaattcggaa tgctcgatcc tatgggccgg gttgggcggc gcgccagccg
     2161 gctgcacgga ttcatgaatg gcaacggttt cgccggtcgc aacaacgccc tcaacgacat
     2221 cgcggctcac cccgccattg gccattttcc gcaccggcat ctcgtcgaac gtgaagacct
     2281 tggattggga taaagcgtcg ctcgccaaat tcgcctcctg tgccgacgcg ccgatgcttg
     2341 tgaccgcgag caataatggg agcgctccca tcaatctgca ataccgcatc ttcgtctccg
     2401 ttggcccgtg ttttccacgg gatcattttc aggctttgtt cggtgctgcg gccaattcgc
     2461 ccagccgcgc cagcgtggat ttggcctgcg ccttgaattt ttcgaattgc atgacgtttt
     2521 cgatgcgcgc ggcgaggaac gcatcggtgg cgctctcgtc ttcgctgtcg tcggcgagcc
     2581 agcgcagcag cgtcgccgaa tagacgcccg ccagaatccc gcgcttggtg tagaaattga
     2641 agtcggtgga aacgtcgccg gcggcccgcc acatcgcatc cacggtgcgg taaaccagct
     2701 cggctgcgag cgccacattc ggcggcaagc tcaaatgcgc gacggcgcgc cgcgccgctt
     2761 ccttgtgagg cttcagaagc gccagccgtg tcttcaccgc ggcggcgatg cgcccccgca
     2821 ccggcatggc ggcgagatcg agcttgccca aacgctcttc catttcggca tcggcgcttt
     2881 cggacgcggc cgccagaagg ccgagcgccc cgtcggggaa aaggcgcagc atgtccgcct
     2941 tgctcgcgcc cgcctccttg gcggcgcggt tcatcagtct gtcggtgaag ccgtcgaacg
     3001 gaatatgggc gagcgccgcc tcgacgacgg cgcctttcag cgccgcatcg tcttttgcca
     3061 cgggtttctc cgtcatggtc attcgccgcc ttttacccag tagcgcgctt cgctctcgaa
     3121 aatcagtttg gtcagatcgc tcatccgctg ggggtagagg atgccgttca gatgatcgca
     3181 ttcgtgctgc acaacgcggg cgtgaaagcc cttggcggtg cgctcgatcg aatgtccttc
     3241 gtgatcgacg ccgcgatagc ggatatgggc cgggcgttcg acgaagccgc gcaggccagg
     3301 gacggaaaga cacccctccc agccgtcttc ggtttccgga ccgagaatct cgatttcggg
     3361 attgatcaac acggtcaggg gcgcggtgcg gtcgaagcgt tcagcttccg aaagaccttc
     3421 ctccgtccgt tcgccgggcg cttggaacac cacgaccctg agcggaacat ggacctgggg
     3481 tgcggctaaa ccggcaccgt cggcgtcgat catggtctcc agcatgtccg ccaccaggcg
     3541 gcggatcgtt ggagcggtag ggtccgcaac cgggtcggcg cggcgcgcca gaaccgggtg
     3601 ccccatccgg gcgattttca gtatagccat ggcagaacta tgagattcag aggcttagcg
     3661 gtcaaatgcc ggctgcccaa ttgaaccttc aaaatcgaag ggggcacccc ccaaggctcc
     3721 agggcgggcg cgccacacgc tgcacctaaa gataaggcgc gcccggcgga tttgaagagg
     3781 tgtggtggac atcacctaac ccttctgtta tctagccgcc cctcaagaaa ccgcgcagga
     3841 gcttgccttt gcagatcatc gttcgcgaca acaatatcga ccaggcgctg aa
//
LOCUS       DASWPU010000301         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_100710,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000301 DASWPU010000000
VERSION     DASWPU010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100710"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /gene="lepB"
                     /locus_tag="VGC27_06440"
     CDS             <1..607
                     /gene="lepB"
                     /locus_tag="VGC27_06440"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY0282244.1"
                     /translation="LIGDYLFVEKFAYGYSKFSFPMGRLLPSFDRILERPARRGDVVV
                     FALPSDPSTVFIKRLIGLPGDRIQMLGGVLYLNDKPVPKVRVADHVEISLDGYEHHVA
                     QYRETLPDGKSYLVLDAVADGPSDNTPVYLVPPGHYFMMGDNRDDSDDSRGIVGYLPA
                     ENLVGKAKIEFFSIDENKTNWWSFWTWPRAIRYSRLLKLVD"
     gene            525..1268
                     /gene="rnc"
                     /locus_tag="VGC27_06445"
     CDS             525..1268
                     /gene="rnc"
                     /locus_tag="VGC27_06445"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY0282245.1"
                     /translation="MKTKRTGGVSGRGRGRSATAACSSSSTDLETRLGHDFADPALLK
                     RALTHASADTAASNERLEFLGDRVLGLICAERLHALYPDDAEGALALKFNALARRDAC
                     AAAAEAAGLAEHIILANAEAGSGGRHKPAILSGACEAVIAALYLDGGMEAARRFVDRY
                     WTEAFAALGSDMRDPKTVLQEWAQSRKAGKGAPVYKLAGREGPDHAPRFEVEVSVAGE
                     ETQSGTGGSKREAEQDAARRMLIMLGLMK"
     gene            1390..>1639
                     /locus_tag="VGC27_06450"
     CDS             1390..>1639
                     /locus_tag="VGC27_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY0282246.1"
                     /translation="MTRCGFAAVIGAPNAGKSTLVNALVGSKVAIVTPKVQTTRMNVR
                     GVAIRGEAQIVLVDTPGIFRPRRRLDRAMVNAAWTGAAD"
BASE COUNT          279 a          537 c          551 g          272 t
ORIGIN      
        1 gctgatcggc gattacctgt ttgttgagaa attcgcttac ggctacagca aattctcgtt
       61 ccccatgggc cggttgctgc cgtctttcga ccgcatcctg gaacgtccgg cccgccgcgg
      121 cgacgtggtc gtgttcgcgc tgccgtccga tccttccacg gtcttcatca agcggctgat
      181 cggcctgccc ggcgaccgca tccagatgct cggcggcgtg ctctacctca acgacaagcc
      241 ggtgcccaag gtgcgcgtgg ccgatcatgt cgagatcagc ctggacggct acgagcatca
      301 cgtcgcccag tatcgcgaga cgctgccgga cgggaaatcc tatctggtgc tcgacgccgt
      361 cgccgacggc ccctccgaca acacgccggt gtatctcgtg ccgccggggc actatttcat
      421 gatgggcgac aaccgcgacg attcggacga cagccggggc atcgtgggct atcttccggc
      481 ggagaacctg gtcggcaagg ccaaaatcga atttttctcg atcgatgaaa acaaaacgaa
      541 ctggtggagt ttctggacgt ggccgcgggc gatccgctac agccgcctgc tcaagctcgt
      601 cgactgatct ggaaacccgg ctcggccatg acttcgcgga cccggcgctt ctgaagcgcg
      661 ctttgacgca cgccagcgcc gataccgccg cctccaacga gcgcctggaa tttctgggcg
      721 accgcgtact cggactgatc tgcgccgaac ggctgcacgc gctctatccg gacgacgcgg
      781 aaggggcgct ggcgctcaaa ttcaacgcct tggcgcggcg cgacgcttgc gccgccgccg
      841 ccgaagccgc ggggctggcc gaacacatca tcctggcgaa cgcggaagcg ggcagcggcg
      901 ggcgccacaa gccggcgatc ctgtcgggcg cctgcgaggc ggtgattgcc gcgctctatc
      961 tcgacggcgg catggaagcg gcgcggcgct tcgtggatcg ctattggacg gaagccttcg
     1021 ccgcgctcgg ctcggacatg cgcgatccca agacggtgtt gcaggagtgg gcgcagtcgc
     1081 gcaaagcggg caagggcgcg ccggtttaca agctggccgg gcgcgaaggc cccgaccacg
     1141 cgccgcgctt cgaggttgag gtcagcgtcg ccggcgagga aacccaatcc ggcaccggcg
     1201 gttccaagcg cgaagcggaa caggacgcgg cccggcggat gctgattatg ttgggcttga
     1261 tgaagtgaaa aaaacctcca aatcacttgg ttcgtcatgg ccgggcttga cccggccatc
     1321 cagcctgtcc cgccgaagcg cttgcgcgat ggcggatcgg cgagcagacg aattggcttg
     1381 gagggcgcgg tgacccgctg cggctttgcc gccgtcatcg gcgcgccgaa cgcgggcaag
     1441 tccacgctgg tcaacgcgct ggtgggctcg aaagtcgcca tcgtcacgcc caaggtgcag
     1501 accacgcgca tgaatgtgcg cggcgtggcg atcaggggcg aggcgcagat cgtgctggtg
     1561 gacacgccgg gcatctttcg cccgcgccgc cgcctcgacc gcgccatggt caatgccgct
     1621 tggacgggcg ccgccgacg
//
LOCUS       DASWPU010000302         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_94430,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000302 DASWPU010000000
VERSION     DASWPU010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94430"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VGC27_06455"
     CDS             <1..1062
                     /locus_tag="VGC27_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0282247.1"
                     /translation="LRGGTEPAAVPPPDVTPAQVALSSESIPAADTGRGGQRDDAAPA
                     EKPRTAQVDRSVAQPPEGPSMPGTLPSPGRTTRVASVAAPVESASLPPPAQTESVAVP
                     PPVPVQSAAIPPPGMQPAVPPTPAVPRQVASIAPSDAALGFQPSTAPPLDPSISQFVA
                     QPILQRYQQTAANAGISSRGAMASEKAIKLRKPRAAERGEGGPETMTGAVVANLDAVQ
                     PAMSVAAPNAYANPQGLPATAIVFFPGDSVYLSAEARAQVRAAADQFKARGGQGYIRV
                     VGHSSSRTADMPLERHLEVIFRKSQERANAVVQELIRGGVPAGKVLMEAVGDSQPVYY
                     ESMPKGEDGNRRVEIFLQG"
     gene            1244..2455
                     /locus_tag="VGC27_06460"
     CDS             1244..2455
                     /locus_tag="VGC27_06460"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF006604.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEY0282248.1"
                     /translation="MQSEFHRIKRLPTYVFEEVNRLKAHARAAGEDIIDFGMGNPDMP
                     TPKHIVDKLCETARDPKSNRYSASRGIKGLRKAMAASYKRRFDITLDPDTEVIAALGS
                     KEGFANLAAAITAPGDVVLVPNPAYPIHAFGFILAGAAIRHIPATSPEAYLSEIGRAA
                     RHSVPSPLAIVVNYPSNPTAQVVDLDFYKEIIAFAKKHEIWVLSDLAYADVYFDDKPP
                     PSILQVEGAKDIAIEFQSLSKTYAMPGWRMGYAAGNRRLIGALARIKSYLDYGAYTPI
                     QVAAAAALNGPQDCVDEIRAIYKSRRDVLVDSMARAGWNVPSPPASMFAWAPIPEQYR
                     ERGSMEFSKQLLIHAKVAVSPGIAFGEYGEGYVRIALVENEHRIRQAARNVKKFLSLR
                     INEPAALEPAK"
BASE COUNT          466 a          872 c          825 g          427 t
ORIGIN      
        1 ttgcgcggcg gcaccgagcc tgcggccgtt ccgccaccgg atgttacgcc cgcgcaggtt
       61 gctttatcct cggaatcgat accggcggcc gacaccggcc gcggcggcca gcgagatgat
      121 gcagcccctg cggagaaacc ccgtaccgca caggttgatc ggtcggtggc tcagcctccg
      181 gaaggcccct cgatgcccgg cactctgcct tctcccggcc gtacgacgcg tgtggcaagc
      241 gtagccgcac cggttgaatc ggccagcctc ccgccgcccg cgcagactga atcggttgcc
      301 gttccgccac ccgtgccggt gcaatcggca gccatacccc cgccaggcat gcaaccggcg
      361 gttccgccga caccggcagt ccctcggcag gttgcctcga ttgccccctc cgatgccgcg
      421 ctgggcttcc agccctccac cgcgccgccg ctcgatccgt cgatttcgca gttcgtcgcg
      481 cagcccattc tccaacgcta tcagcagact gcggcaaatg cgggcatttc ctccaggggg
      541 gccatggcga gcgaaaaggc gatcaagctc cgtaagccgc gggcggccga aaggggcgaa
      601 ggcggaccgg aaaccatgac cggcgcggtc gtggccaatc ttgacgccgt tcaaccggcc
      661 atgagcgtgg ccgcgccgaa cgcctatgcc aatcctcagg ggctgccggc gaccgccatt
      721 gtcttcttcc ctggcgattc ggtctatctg agcgctgaag cgcgcgcgca ggtgagggcg
      781 gcggcagacc agttcaaggc caggggcggc caaggctata tccgcgtggt gggccattcc
      841 tccagcagga cggcagatat gcctttggaa cgccacctgg aggtcatttt ccgcaaatcg
      901 caggagcggg ccaacgcggt ggtacaagaa ctcatccgcg gcggcgttcc ggctggcaaa
      961 gtgctcatgg aggccgttgg cgacagccag ccggtctatt acgagtcgat gccgaaaggc
     1021 gaagacggga accgccgggt ggagatattc ctgcagggct gactctgggc gcgaccgtgc
     1081 ttccggctcc gctttgcggc gcatggcaga gtgacggcgg cacctataag tgacgctatg
     1141 cccaagcaag gcatggcagg aacaccctga aagccgttga cgcggcgggt aaattgtcgc
     1201 gatgctgcgg ccgcgatttt atttcgcgct taagggttcc gacatgcaga gcgaattcca
     1261 ccgcataaag cggctgccga cctacgtctt cgaggaagtg aaccgtctga aggcgcacgc
     1321 gcgggcggcc ggcgaggaca tcatcgattt cggcatgggc aacccggaca tgccgacgcc
     1381 caagcacatc gtcgacaagc tgtgcgaaac ggcgcgcgac ccgaagtcga accgctactc
     1441 cgcctcccgc ggcatcaagg gcctgagaaa ggccatggcc gcctcttaca agcgccgttt
     1501 cgacatcacc ctcgatcccg ataccgaagt catcgcggcc ctcggctcga aggaaggctt
     1561 cgccaacctc gccgcggcca tcaccgcgcc cggcgacgtg gtgctggtgc cgaacccggc
     1621 ataccccatc catgcattcg gcttcatcct ggcgggcgcc gccatccgcc atattccggc
     1681 gacatcgccc gaagcttatt tgagcgaaat cggacgcgcc gcgcgccatt ccgtgccttc
     1741 gccgctggcg atcgtggtga actatccgtc caatcccacg gcgcaggtgg tggacctcga
     1801 tttctacaaa gagattatcg ccttcgcgaa gaagcacgaa atctgggttc tatcggacct
     1861 cgcttatgcc gacgtctatt tcgacgacaa accgccgccg tcgatcctgc aagtcgaagg
     1921 ggccaaggac atcgccatcg agttccagtc cctgtcgaag acctacgcga tgccgggctg
     1981 gcgcatgggc tatgccgccg gcaacaggcg gttgatcggg gcgctggcgc gcatcaaatc
     2041 ctatctcgat tacggcgcct acacgccgat ccaggtcgcc gccgccgccg ctctgaacgg
     2101 gccgcaagac tgcgtcgatg aaatccgcgc catctacaag tcgcggcgcg acgtgctggt
     2161 ggacagcatg gcgcgcgccg gctggaacgt gccgtcgccg cccgcctcga tgttcgcctg
     2221 ggcgccgatt ccggaacaat atcgcgagcg cggctcgatg gagttctcca agcagcttct
     2281 gatccacgcc aaggtcgcgg tctcgccggg catcgccttc ggcgaatacg gcgaaggcta
     2341 tgtccgcatc gcgctggtgg agaacgaaca tcgcatccgt caggccgcgc gcaacgtgaa
     2401 gaagttcctg tcgctgcgga tcaacgaacc ggccgcattg gagcccgcga aatgaacggc
     2461 gtccgcatcg cccttgcggg gctgggcacg gtcggcgcgg gagtcggcaa ggcgcttgaa
     2521 gcgcatggcc ccgaattgag cgggcgggcg ggggtgccgc tggcggtcgt cgccgtcagc
     2581 gcgcgcgaca
//
LOCUS       DASWPU010000303         4729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_51594,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000303 DASWPU010000000
VERSION     DASWPU010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51594"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VGC27_06465"
     CDS             complement(<1..692)
                     /locus_tag="VGC27_06465"
                     /inference="COORDINATES: protein motif:HMM:NF036808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282249.1"
                     /translation="MTYPTIRVKPREGRRARAGAPWLFSNEIEMPAKTLAPGAVVNVT
                     GDDGRVFGTGYFNPKSLIAVRLLDAAADVALDAAFLAAKLKRPLALRETLYRAPYYRL
                     VNAEGDGLPGLTIDRFADTFVVQVATAGMEALLEPLLAALDETLHPAQVVLRADVPAR
                     ALEGLESYVRNAKGEAGRIAVEENGVRYFADLAAGQKTGWYFDQRDNRAPYYRLVNAE
                     GDGLPGLTIDRFA"
     gene            complement(781..1026)
                     /locus_tag="VGC27_06470"
     CDS             complement(781..1026)
                     /locus_tag="VGC27_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0282250.1"
                     /translation="MITAAQLRAARALLAMDQRQLAEIAGLSVPTIQRMEASEDVIRG
                     NVDSLMKLIAALNGAGVELIGEGAISAEGGRGIRLKK"
     gene            1206..1574
                     /locus_tag="VGC27_06475"
     CDS             1206..1574
                     /locus_tag="VGC27_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282251.1"
                     /translation="MHLKPALRLGGWGDNKTRSLGEAARLAHALADAEALPIGKTLAR
                     EGHGAGTDGWYAESILHTVPVYGTSTAAPERLDAAALAGLHLSEGAETLDGDAAPVWR
                     NLQVKREDFARYVEWLRSVW"
     gene            complement(1702..1777)
                     /locus_tag="VGC27_06480"
     tRNA            complement(1702..1777)
                     /locus_tag="VGC27_06480"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1742..1744),aa:Thr,seq:cgt)
     gene            1883..3205
                     /locus_tag="VGC27_06485"
     CDS             1883..3205
                     /locus_tag="VGC27_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HEY0282252.1"
                     /translation="MITRRDAALGGVLAAGSLAAWPGGLSAKPKKSFVRVDAQRFVLD
                     GATYRFAGTNIWYGAYLGANGRDRLRRELDAIAGLGLTNLRVLGASELSPLKNSLRPA
                     FRAAKPPYNQRLLKGLDFLLAEMGKRQLKAVVFLNNFWEWSGGMMTYLYWTNGGHYID
                     LGDPAHPWPAFADFSARFYSSPEAVALSLHYAGDLIARRNSVTGILYRDDPAIMAWQL
                     ANEPRPGGSEAFGRANLPAYYAWIAQAAKFIKARDPNHLVTTGNEGLMGSLNIKEAVV
                     KAHAIPEIDYMTFHLWPLNWSWIDPKNLPATYASAEAKGRAYVKAHIAIAKGLNKPLV
                     AEEFGFPRDQGYEPGSPTSFRDAFYTMLYGEVLDSAKRGGPFAGTNFWAWGGSGRAQH
                     KDYLMRPGDLSYVGDPPQEPQGANSVFDADATTLRIVRAHVAALNAAG"
     gene            3274..4479
                     /locus_tag="VGC27_06490"
     CDS             3274..4479
                     /locus_tag="VGC27_06490"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0282253.1"
                     /translation="MKRIASRLSMSHAASGLVSGVFTPFFGAWLAWRGLSPDQIAILL
                     SAGLLLRAFVGPASGIVADARNDRRAVMLWLYWAMLIGYGALNFTAAPLIIFLAAVPA
                     TVANGAALPLLESVSVRLSERYGFSYGRVRLWASSAFVAANILGGACVWWFGMGSVAV
                     MLAAGAVLCVITTVILPAPPLDQARGDLSLRLRTTFGEARELLRSGVFLIFLAATSFD
                     QGSHAFYYGYGGLHWHFLGYSGLLIGILWPLGVFAEIALFSQSLRVLRVIGPTRLLFY
                     GALGCAVRWTILAFDPPLPLVVFAQILHGATFALAHLGAMYFILRAVPSRLAATAQSL
                     YFVCSAGIVMGIATFASGHIYAAYGGRAYLLMSAMGVAAMGLALWLARRWQGERILHG
                     AHDERLDTI"
     gene            complement(4592..4726)
                     /locus_tag="VGC27_06495"
     CDS             complement(4592..4726)
                     /locus_tag="VGC27_06495"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY0282254.1"
                     /translation="MRLSAGAEFVVAYAGEILTMPGLPRVPAANAIHLNDKGEIEGLF
                     "
BASE COUNT          794 a         1549 c         1493 g          893 t
ORIGIN      
        1 gcgaaacggt cgatggtgag tccgggcaag ccgtcgccct cggcattgac caggcgataa
       61 taaggggcgc ggttgtcgcg ctgatcgaag taccagcctg tcttctgtcc cgccgccaga
      121 tcggcgaaat agcgcacgcc gttttcctcc accgcgatgc gccccgcttc gcccttcgcg
      181 ttgcggacat aggactccag cccctccagc gcgcgcgccg gaacgtcggc gcgcagaacc
      241 acctgcgcgg ggtgcagcgt ttcgtccagc gccgccagca gcggttcgag cagcgcttcc
      301 attccggcgg tggcgacctg cacgacgaac gtatcggcga aacggtcgat ggtgagtccg
      361 ggcaagccgt cgccctcggc attgaccagg cgataataag gggcgcggta aagcgtttcg
      421 cgcagcgcca gcggccgctt cagcttggcg gcaagaaacg ccgcatcgag cgcgacatcg
      481 gcggcggcat cgagcaggcg caccgcgatc aggctcttgg ggttgaaata gccggtgccg
      541 aagacgcgcc cgtcgtcgcc ggtcacgttc accactgcgc ccggcgccag ggtcttggcc
      601 ggcatctcga tctcattgga gaacagccag ggggcgccgg cgcgcgcgcg gcggccttcg
      661 cgaggcttca cgcggatggt gggataggtc atagcggctc aggtagcacg gctgagaaga
      721 aacaactatc cggcaaaggc tcgaagagaa gccagggcgg cgcggcgccc catccggccg
      781 ttatttcttc aatctgatcc cgcgcccgcc ttcggcgctg atcgcgcctt cgccgatcag
      841 ttcgacaccc gcaccgttca gggccgcgat caacttcatc agcgaatcca cattgccgcg
      901 gatcacgtcc tcgcttgctt ccatgcgctg gatggtcggc accgacagtc cggcaatttc
      961 ggcaagctgt cgctgatcca tggcgagcag cgcgcgcgcc gccctgagct gtgccgccgt
     1021 gatcatgcgt taaccatccc ctggagcgct ccaagccgca cttgtgtctt ggcagacact
     1081 cttttgttgg ttggccggta acaatacatg tcttacatat cacattgacc ggtctataag
     1141 gccgaccggc cgccaagtga ggaatgcccc aagcatcgcg aaccatgctt cttacaggat
     1201 tatctatgca tctcaagccg gccctccgcc tagggggctg gggggataac aaaacccgct
     1261 cgcttggcga ggcggcccgt ctggcgcacg ccctggccga cgccgaagcc ttgccgatcg
     1321 gcaaaacctt ggcgcgagaa ggacatggcg ccggcaccga cggttggtac gccgagagca
     1381 tcctgcatac ggtgccggtc tatggcacca gcaccgccgc gcccgaacgc ctcgacgctg
     1441 ccgcgcttgc cggcctgcac ctgagcgaag gcgccgagac tctggacggc gatgcggcgc
     1501 cggtgtggcg caatttacag gttaagcggg aagacttcgc ccgttatgtc gaatggctgc
     1561 gcagcgtctg gtaacggcta taccatgcag aatgaagatg agatcaggga tggcgtaagc
     1621 cagccgaagc tcgcagcaac cagtccacct tcgcccttcg ggctacggtg gacagccttc
     1681 tctcgcttcg cgagcgaagg ctggtgccgc ctgggtgact cgaacacccg acccccgcat
     1741 tacgaatgcg gtgctctacc ggctgagcta aggcggccct tgccccatca cagggcgggc
     1801 gaaaatacgc aggcggagag cctcctgcaa cagcggatat gcgttagtct ggggcaggcg
     1861 gccgttttgc ggggaacatt tcatgattac cagaagggat gcggcgctgg gaggcgtttt
     1921 ggcggccggt agtctggcag cctggccggg cgggctttcg gcaaaaccga agaagagctt
     1981 cgttcgcgtg gacgcacagc gcttcgttct ggacggcgcg acctatcgct tcgccggcac
     2041 gaatatctgg tacggagcct atctgggtgc gaacggccgc gaccggctgc gccgggaact
     2101 cgacgcaatc gccggcctcg ggctgaccaa tctcagggtg ctcggagcct cggaattgtc
     2161 cccgctcaag aactcgctgc gtcctgcctt tcgcgccgcc aagccgcctt acaaccagag
     2221 gctcctcaag ggcctcgact tcctgctggc cgaaatgggc aaacgccagc tcaaagccgt
     2281 ggtctttctc aacaatttct gggaatggtc gggcggcatg atgacctatc tctattggac
     2341 caatggcggc cattacatcg atttgggcga ccccgcccat ccttggccgg cgttcgccga
     2401 cttctcggcg cggttctaca gcagcccgga ggcggtggca ctttcgctcc actatgccgg
     2461 cgacctgatc gcaagacgca acagcgtcac cggcatcctc taccgcgacg acccggccat
     2521 catggcttgg cagctggcca acgagccgcg ccccggcggc agcgaagcgt tcggccgcgc
     2581 gaatttgccc gcctactatg cctggatcgc gcaagccgcg aagttcatca aagcccgcga
     2641 ccccaatcat ctggtgacaa cgggaaacga agggctgatg ggcagcttga acatcaaaga
     2701 agccgtcgtg aaggcgcacg ccatcccgga gatcgattac atgacgttcc atctttggcc
     2761 gctgaactgg agctggatcg accccaagaa ccttcccgcc acttacgcat cggccgaagc
     2821 caaaggccgt gcttacgtca aggcgcatat cgccatcgcc aagggactga acaagccgct
     2881 ggtggccgaa gagttcggct ttccgcgcga tcaaggctac gaacccggct cgccgacctc
     2941 cttcagggat gcgttctaca ccatgctcta cggcgaggtt ctcgacagcg ccaaacgcgg
     3001 cgggcctttc gccggtacca atttctgggc ttggggcgga tccggacgcg cgcagcacaa
     3061 ggattatctc atgcggcccg gcgacctctc ctatgtcggc gatccgccgc aggagccgca
     3121 aggcgcgaat tcggtgttcg acgccgacgc caccacgctg cggatcgtcc gcgcccatgt
     3181 cgccgcactg aatgcggccg gctgaccgtt ttctttccgc gccggacgcg ccatggtgac
     3241 gaaaagccgt ccgccttccc ggaaagagta ttagtgaaaa gaatcgccag ccgcctgagc
     3301 atgagccatg ccgcttcggg cttggtcagc ggcgtgttca cgccgttctt cggcgcctgg
     3361 ctcgcctggc gcggactttc acccgaccaa atcgccatcc tcttgtcggc gggcctgttg
     3421 ctgcgcgcct ttgtcgggcc ggcgagcggc atcgtcgccg acgcgcgcaa cgaccggcgc
     3481 gcggtgatgc tctggctcta ttgggcgatg ctgatcggct atggcgcgct caatttcacc
     3541 gccgcgccgc tcatcatctt cctggccgcc gttcccgcca cggtggcgaa cggggccgcg
     3601 ttgccgctgc tcgaaagcgt cagcgtgcgc ctgtccgagc gctacggctt cagctacggc
     3661 cgcgtccggc tgtgggcgtc gtcggcgttt gtcgcggcga acattctggg cggcgcctgc
     3721 gtctggtggt tcggcatggg aagcgtggcg gtcatgcttg ccgccggcgc ggtgctgtgc
     3781 gtgatcacga ccgtcatcct tcccgcgccg cctttggacc aggcgcgcgg cgatctttcc
     3841 ctgcggctgc gcacaacctt cggcgaggcg cgcgaattgc tgcggtcggg cgtgttcctg
     3901 atcttcctgg cggcgaccag cttcgaccag ggcagccacg ccttctatta cggttatggc
     3961 gggctgcact ggcattttct gggctattcc ggcctcttga tcggaatcct ttggccgctc
     4021 ggcgtgttcg ccgagatcgc gctgttctca cagtcgctgc gcgtgctgcg cgtgatcgga
     4081 ccgacacggt tgctgttcta cggtgcgctg ggctgcgcgg tgcgctggac cattctggcc
     4141 ttcgatccgc cgctgccgct ggtggtgttc gcgcaaatcc tgcacggcgc gactttcgcg
     4201 ctggcgcatc tgggcgcgat gtatttcatc ctgcgcgccg tgccctcacg gttggccgcc
     4261 acggcgcaga gtctttattt cgtctgttcg gcggggatcg tgatggggat tgcgaccttc
     4321 gcctccggtc acatctacgc cgcctatggc ggacgcgcct atcttctgat gtcggcaatg
     4381 ggcgtggcgg cgatgggact ggcgttgtgg ctggcgcggc gctggcaggg cgagcgcatc
     4441 ctccacggcg ctcacgacga acgcctcgat acgatctgaa atccgagttt gtcttggatt
     4501 gagcttcctg actgccgccg ccttgtcatg gcctgcaaaa gcaggccatg acaagtgaga
     4561 ataattgcga ttccggtaaa tccaaacccc tctaaaacag cccttcgatc tcgcccttgt
     4621 cgttgaggtg gatcgcgttt gccgccggca cgcgtggcag gccgggcatg gtcaggatct
     4681 cgccggcata ggccacgacg aattccgccc ccgccgacag gcgcacgtc
//
LOCUS       DASWPU010000304         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_106754,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000304 DASWPU010000000
VERSION     DASWPU010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106754"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VGC27_06500"
     CDS             complement(<1..661)
                     /locus_tag="VGC27_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282255.1"
                     /translation="MRAAVALCFLCAAMPAAAQENGDLDRIPTDLPDAPAASRPAANA
                     HGKYYLENAFGLSSARGDLAVPLPSSSSSAWGNRTSLDAFDQWPLAQNLSFTFSDRFS
                     FTENDGIVFPRDAARNDLREAYFTWEPLTQTFLEFGRVNLKNGIALGFNPTDFFKTRS
                     AVALASADPSELRGDRLGTLMLRAQTIWDGGSATFVYAPKLHTPRPLAGAAPGWIDPG
                     FD"
     gene            complement(661..1437)
                     /locus_tag="VGC27_06505"
     CDS             complement(661..1437)
                     /locus_tag="VGC27_06505"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEY0282256.1"
                     /translation="MKNLLTGFAVALLLVAGARAATAPTAPDIVAGVDKLRNPGQPFR
                     ITNTMIEYVGGKPRDRVTLVVFAKENKQTRQFSNLVRYIDPPRDLGKMVLMNGSNMWF
                     HDPSSKVSVRISPQQRLMGQAANGDVMTANIAKDYTATLLGEETLQDADRKDRACWHL
                     DLKAATDEAVYSRVEYWVEKGSYRPVKGKFYSDSGRLLKIAYYRKYVEQLGAMRASET
                     IIIDSVDQNLVTTMTTSDMRAQDIPDAWFQREYLPRLKAD"
     gene            complement(1448..>2307)
                     /locus_tag="VGC27_06510"
     CDS             complement(1448..>2307)
                     /locus_tag="VGC27_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0282257.1"
                     /translation="SPPKKEQTVLTDAPVMEVKLAELSARDRDTGQSAPADTAPRIDL
                     LGATAGGSPNVVSFFVNEAQSQGYKELDDAYVGMNIKLAQQLLYGRGEHKAVSISIQL
                     HRSEDLGKARARLNTLIRQHGLDLEVHDFTELQPQYLQIIGFMSAIFTFIAVIMVVIV
                     LFTVVNTMSMSVMERTNEIGTARAMGVRRSGIRRQFLIEGWMLGVIGATGGVILAAVI
                     AHWFNSLGITYTPPGQATPVPLWLLTQGVWGLQLSVWFGLLVMATLAAFIPANRAARM
                     LVVDALRHV"
BASE COUNT          418 a          715 c          760 g          414 t
ORIGIN      
        1 gatcgaaacc cggatcgatc cagcctggcg cggcgcccgc cagcggcctc ggcgtgtgca
       61 gtttgggcgc gtaaacgaac gtcgccgaac cgccgtccca aatggtttgc gcgcgcagca
      121 tcagcgtgcc gagccggtcg ccgcgcaact ccgagggatc ggccgaggcc agagccaccg
      181 cactgcgggt cttgaagaag tcggtcggat tgaagcccag cgcgatgccg ttcttgagat
      241 tgacgcggcc gaattccaga aaagtctgcg tcaacggttc ccaagtgaag taggcctcgc
      301 gcagatcgtt gcgcgcggcg tcgcgcggaa acacgatgcc gtcgttctcg gtgaaactga
      361 agcgatcgct gaaggtaaag gacaggttct gagccagcgg ccactggtcg aacgcgtcca
      421 ggctggtgcg gttgccccaa gcggacgaag aggacgacgg caaagggacc gccagatcgc
      481 cgcgcgccga cgacaagccg aaggcgttct ccaaataata tttgccgtgt gcgttcgcgg
      541 ccggccgcga ggcggccggc gcgtccggca ggtccgtcgg aatgcggtca aggtcgccgt
      601 tttcctgcgc cgcggccggc atcgccgcgc acagaaagca caaggcgacg gcggcgcgca
      661 tcaatcggcc ttgaggcgcg gcagatattc gcgctggaac caagcgtccg ggatgtcctg
      721 ggcgcgcatg tcggaggtcg tcatcgtggt caccagattc tggtccacgg aatcgatgat
      781 gatggtttcg ctcgcgcgca tggcgccaag ttgttcgaca tatttgcggt aataggcgat
      841 tttcagcagc cgcccgctgt cggagtagaa cttgcccttc accgggcgat agctgccctt
      901 ctccacccaa tactccaccc ggctgtaaac cgcttcgtcc gtcgccgcct tgaggtcgag
      961 gtgccagcag gcgcggtcct tgcggtcggc gtcttgcaat gtctcctcgc cgaggagcgt
     1021 tgccgtgtaa tccttggcga tgttcgccgt catcacatcg ccgttggcgg cttgccccat
     1081 cagacgctgc tgcggcgaga tgcggacgct gaccttggac gacggatcgt ggaaccacat
     1141 gttcgaaccg ttcatcagca ccatcttgcc gagatcgcgc ggcggatcga tgtagcgcac
     1201 cagattgctg aattggcggg tctgcttgtt ctccttggca aagaccacca gggtgacgcg
     1261 gtcgcgcggt ttgccgccga catactcgat catcgtgttg gtgatccgga agggctggcc
     1321 gggattgcgc agcttgtcca cgccggcgac gatgtccggc gcggtcggtg cggttgcggc
     1381 gcgcgcgccc gccaccaaca gaagagccac ggcgaatccg gtcagcaggt tcttcatcga
     1441 tcgatcctca aacatggcgc agcgcatcga cgacgagcat gcgcgcggcg cggttggccg
     1501 gaatgaaggc ggcgagcgtc gccatcacaa gaagcccgaa ccagacggaa agctgcaagc
     1561 cccacacgcc ttgcgtgagc agccacaggg gaacgggagt cgcctggccc ggcggcgtgt
     1621 aagtgatgcc caggctgttg aaccagtgcg cgatcaccgc cgccaggatc acgccgccgg
     1681 tggcgccgat gacgccgagc atccagcctt cgatcaggaa ctgacggcgg atgccgctgc
     1741 ggcgcacgcc catggcccgc gccgtgccga tctcgttggt gcgctccatg acgctcatgc
     1801 tcatggtgtt gacgacggtg aacagcacga tcaccaccat gatgaccgcg atgaaggtga
     1861 aaatcgcgct catgaaaccg atgatctgaa ggtattgagg ttgcagctcg gtgaaatcgt
     1921 gtacttccag gtcaagacca tgctggcgga tcagcgtgtt cagacgggcg cgggccttgc
     1981 ccaagtcttc actgcgatgg agctggatgg agatgctcac cgccttatgc tcgccgcggc
     2041 cgtaaagaag ctgctgggcc agcttgatat tcatgccgac ataggcgtcg tccagttcct
     2101 tgtaaccctg gctttgagct tcgttcacga agaagctgac gacgttgggc gagccgcccg
     2161 cggtggcgcc gagaagatcg atgcgcggcg ccgtgtccgc gggcgcgctc tggccggtgt
     2221 cgcggtcgcg ggccgaaagc tccgccagct tcacctccat cacgggcgca tcggtcagca
     2281 ccgtctgctc tttcttgggc ggcgacg
//
LOCUS       DASWPU010000305         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_75979,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000305 DASWPU010000000
VERSION     DASWPU010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75979"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /locus_tag="VGC27_06515"
     CDS             complement(<1..1682)
                     /locus_tag="VGC27_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="strawberry notch family protein"
                     /protein_id="HEY0282258.1"
                     /translation="MNTMIPAAALAVAIPTPDAFPIIHKAAEQLLGPVSRGEVVSSDL
                     LRTAMTAAFGGSDAEGLWDWKSAYDVCEVVQILFLRKFGSAIRARAASPAAELAMHTR
                     IASLFPTHTRRSEESQTLQQFSTPLPLAFVAAQAAAITPADLVLEPSAGTGLLAIFAE
                     LAGAGLALNELADNRAFLLRGLFPTASVTQHDAAHIHDFLDASVQPSVMLMNPPFSAA
                     AHVDGRVAGAAMRHIASALARLADGGRLVAITGANLAPDNLAWRDAFVRLQEHARVVF
                     SAAIDGRVYARHGTATDTRLTVIDKVPAVDPQILPPSRGMAPDATTLLGWIAELPSRA
                     LATVSQHAAQSAVRGAVRRPSTRIAVSRSPTEITGVELAYETADWTASDHGHLGESIY
                     ESYALQSIRIPSAPQHPTKLVQSAAMASVAPPKPTYRPRLPSIVTDRGLLSDAQLESI
                     IYAGEAHTAFLAGAWTVDETFDVVSAAPDSAEASVRFRRGWMLGDGTGAGKGRQVAGI
                     LLDNWCQGRTRAVWIYKSDKLIEDAQRDWSALGQERLLVTPLSRFRQGTAIRL"
BASE COUNT          357 a          558 c          640 g          284 t
ORIGIN      
        1 agacggatcg ccgtgccctg cctgaagcgt gacagtggcg tgaccaacaa acgctcctgt
       61 ccgagagccg accaatcgcg ttgtgcgtcc tcgatcagct tatcggattt gtagatccac
      121 accgcgcggg tgcggccctg gcaccaattg tcgagcagga tgcccgcgac ctggcggcct
      181 ttgccggcgc cggtgccgtc ccccaacatc cagccgcggc ggaagcggac cgaggcttcg
      241 gcgctatctg gtgccgccga gacgacatcg aaggtctcgt ccaccgtcca tgcacccgcg
      301 agaaacgcgg tatgcgcctc acccgcatag atgatgcttt cgagctgagc atcggaaagc
      361 aggccacggt cagtgacgat cgatggcagg cgagggcgat aggtgggctt cggcggggcg
      421 accgacgcca tagcggccga ttggacgagc ttggtcgggt gctgtggagc gcttggaatg
      481 cgaatggact ggagcgcata gctctcatag atcgactcgc ccaaatggcc gtgatcggac
      541 gcggtccagt cggcagtctc gtacgcgagt tcaacgccgg tgatttcggt tggcgaccgt
      601 gaaaccgcaa tgcgggtcga aggccgacgc actgcgccgc gcacagcgga ctgcgcggcg
      661 tgttgggaga ctgtggctag cgcgcgcgac ggaagctcgg caatccagcc gagcagcgtc
      721 gtggcatcag gagccatgcc gcgcgaagga gggagtattt gcgggtcaac cgctgggacc
      781 ttgtcgatca cggtgaggcg cgtgtcggtc gccgtgccat ggcgcgcata aacccggcca
      841 tcgatggcgg ccgagaacac cacacgggcg tgctcctgca acctcacgaa cgcgtcgcgc
      901 caggcaaggt tgtcgggcgc gagattagcg cccgtgattg caaccagccg accgccatcg
      961 gcgaggcgcg caagcgccga agcgatgtgg cgcatcgcgg cgccggcgac gcgaccgtcg
     1021 acatgggccg cggccgagaa cggtgggttc atcagcatca cgcttggctg cactgacgca
     1081 tcgagaaaat cgtggatgtg cgcggcgtcg tgttgggtca ccgacgccgt cggaaacaac
     1141 ccacgcaaca ggaaggcgcg attgtcggcg agttcgttga gcgcgagccc ggcaccggcg
     1201 agttcggcaa agatcgcaag caaccccgtg ccggcagacg gttcgagtac cagatcggca
     1261 ggcgtaatgg ccgcggcttg cgcggcgaca aacgccaaag gcagcggcgt cgagaattgc
     1321 tgcaaagttt ggctctcttc ggagcggcgg gtgtgggtgg gaaagagaga agcgatgcgt
     1381 gtgtgcatcg cgagctcggc ggccggagag gcggcacgcg cgcgaatggc gctgccgaat
     1441 ttgcgcagga acaggatctg cacgacctcg cagacgtcgt aagcggactt ccagtcccac
     1501 aggccttcgg cgtcgcttcc gccgaaggcc gcggtcatcg cggtgcgcaa gagatcagaa
     1561 gaaaccacct ccccgcgcga gaccggcccg agcagctgct cggcagcttt gtggatgatg
     1621 ggaaacgcat caggcgtggg tatcgcgacc gcgagcgcag ccgcagggat catggtgttc
     1681 atagggaagg ccttaaggag agcggagatg ggcgaaccgg gaggcgctct cttgagcctg
     1741 accagttcag acccgtcccg gccgtcctct cactcttgcg gatcgatcgg cacaagatgc
     1801 caatgccagc cgccattccc gcccaaaacc aggaatggg
//
LOCUS       DASWPU010000306         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_64137,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000306 DASWPU010000000
VERSION     DASWPU010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64137"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VGC27_06520"
     CDS             complement(<1..607)
                     /locus_tag="VGC27_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282259.1"
                     /translation="MTMMRGMGAFVLAAALAVPAAAADYVWRNVKVGGGGYIPAIVYS
                     KVEKNLAYLRTDMGGIYRWEAGRQKFLPLEDNIAERNFQGIESIAPDPKEGNVVYAAI
                     GTYRNGPAAIFRSEDRGDHWTINPVPFRMGGNEEGRDLGERLAVDPNATSILYFGSRH
                     DGLQKSTDKGKTWARVASFPHQGLGTPAAHEPTHAGLSFVVF"
     gene            731..>2699
                     /locus_tag="VGC27_06525"
     CDS             731..>2699
                     /locus_tag="VGC27_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEY0282260.1"
                     /translation="MRCLHAAAKGVVLALGLSCAPVCFGAAWASDPLTLWYDRPATSW
                     EREALPIGHGAMGAMLSGGVAVDDIQFNEKTLWTGGPGAKGGYDFGWPAQPQAEKVAG
                     VQAKLAAETRLEPEAVAQILGHDIVGYGDYQSFGNLILSMKNAGGAVTGYRRSLDIAK
                     AMGELAYSQDGVSYRRQYFASYPDSVIVVRLTADKPGRIAFSAKLTVPDNRSAAFAAR
                     DGRITVAGALHDNGLKYEAQLQVLPQGGTRSDNADGTVTVAGADAAMLILSAGTDYRL
                     HYPDYRGADPHDAVTAKLTKAVAKSYEALLAAHAKDYEALFGRLQIDLAGEMPDVPTD
                     ALLAGYGKADPAARRALEALHFQFGRYLLIAASRPGSLPANLQGVWNESKTPPWNADY
                     HVNINLQMNYWLAETGNLPGTAEPLIDFVEALVPPGEVAAGRILGSKGWTLFLNTNIW
                     GHTGLIAWPTAFWQPEAGAWLASQLYDHYRFTQDAAYLKRLYPILKGAAATWLDALIA
                     DPRDGRLVVSPSYSPEHGPFSAGAAMSQQIVEQVFRDAGEAADAMGDTAFAARLKAAR
                     DRLDPGLKIGRFGQLQEWKEDWDDPQDEHRHVSQLYALHPGNRIAPATEPALAAAARA
                     TLKARGDGGTGWSKAWKINFWARLGDGDHAHS"
BASE COUNT          458 a          859 c          918 g          464 t
ORIGIN      
        1 cgaagacgac gaagctgagc ccggcatggg tgggttcgtg cgccgcgggc gtacccaggc
       61 cttggtgcgg gaaactcgcc acgcgtgccc aggtcttgcc cttgtcggtg ctcttctgca
      121 ggccgtcatg gcgcgagccg aaatagagga tgctcgtcgc gttgggatcg acggcgaggc
      181 gctcgcccag atcgcggcct tcctcgttgc cgcccatgcg gaaggggacc gggttgatcg
      241 tccaatgatc gccgcggtct tcggaccgga agatcgccgc cgggccgttc cggtaggtgc
      301 cgatggcggc gtagacgacg ttgccctctt tgggatcggg ggcgatgctc tcgatcccct
      361 ggaaattgcg ctcggcgata ttgtcctcga gggggaggaa tttctgccgc cccgcttccc
      421 agcgatagat gccgcccatg tcggtgcgca gataggcgag attcttctcc accttgctgt
      481 agacgatggc ggggatatag ccgccgccgc ccaccttgac gttgcgccag acgtaatcgg
      541 ccgccgccgc cggcacggcc agcgccgccg ccagcacgaa cgcgcccatc cctctcatca
      601 tggtcatgat gcgtttctcc ctttggactg cggcggaaaa acctgcccgc ccacgccggt
      661 ttctgctatc gttgggaaaa atcatataat taccgccatg ctgaaggcaa tcacatgtca
      721 gggaggaaga atgcggtgtt tgcacgctgc cgccaagggc gtggtcctgg cgcttggcct
      781 tagttgcgct ccggtctgct ttggggctgc gtgggcaagc gatccgctga cgctgtggta
      841 cgacagaccg gcaacgagtt gggagcgcga ggccttgccg atcggccacg gcgcaatggg
      901 ggcgatgctg tccggcggcg tggcggtgga cgatatccag ttcaacgaaa agacgctgtg
      961 gaccggcgga ccgggcgcca agggtggtta cgatttcggc tggccggcgc agccgcaggc
     1021 ggaaaaggtc gcgggcgtgc aggcaaagct cgcggccgaa accaggctgg agcccgaggc
     1081 ggtggcgcag atcctgggcc acgacatcgt tggctatggc gactaccaga gcttcggcaa
     1141 tctgatcctc tccatgaaga acgccggcgg ggctgtgacg ggctaccgcc gcagtctcga
     1201 catcgccaag gccatgggcg aactcgctta ctcgcaggac ggcgtgtcgt atcgccggca
     1261 gtatttcgca tcttatcccg atagcgtcat cgtggtgcgg ctgacggcgg acaagcccgg
     1321 ccgcatcgcc ttttccgcca agctgacggt gcccgacaat cgcagcgccg cgtttgccgc
     1381 cagggacggg cgcatcacgg tcgccggcgc gctccatgac aacggcctga aatacgaagc
     1441 ccaattgcaa gtgctgccgc agggcggtac gcgcagcgat aacgccgacg gcacggtgac
     1501 ggtggcgggt gccgatgcgg cgatgctcat cctgtcggcc ggcaccgatt accgcctgca
     1561 ctatcccgac taccgcgggg ccgatccgca cgatgcggtg acggcgaagc tgacgaaggc
     1621 cgtggctaaa tcctatgagg cgctgcttgc ggcccatgcg aaggactatg aggcgctgtt
     1681 cggtcgcttg cagatcgatc tcgcgggcga aatgcccgat gtgccgaccg atgcgctgct
     1741 cgccggctat ggcaaggccg atcccgccgc ccggcgcgcg ctcgaagccc tgcatttcca
     1801 gtttggccgg tatctgttga tcgccgcgtc gcggccgggc agtttgccgg ccaatctgca
     1861 aggcgtgtgg aacgagtcga agacgccgcc ttggaatgcc gactatcacg tcaacatcaa
     1921 tttgcagatg aactattggc tggcggagac gggcaatctt ccgggtacgg cagagccgct
     1981 gatcgatttc gtcgaagcgc tggtgccgcc gggcgaagtg gcggccggcc gcatcctcgg
     2041 cagcaagggc tggacgctgt tcctcaacac caacatctgg ggccataccg gcctgatcgc
     2101 ctggccgacg gccttctggc agccggaggc gggcgcctgg ctcgcctcgc aactctacga
     2161 ccattaccgc ttcacgcagg atgcggccta tctcaagcgg ctctatccca tcttgaaagg
     2221 ggcggcggcg acgtggctgg acgcgctgat cgccgatccg cgcgacggca ggctggtggt
     2281 ctcgccgtcc tattcgcccg aacacgggcc gttctccgcc ggagcggcga tgtcgcagca
     2341 gatcgtcgag caggtgttcc gcgatgccgg cgaagcggcg gacgcgatgg gcgacaccgc
     2401 ctttgcggcg cggctcaagg cggcgcgaga ccgcctcgat cccgggctca agatcggccg
     2461 cttcggacaa ttgcaggaat ggaaagaaga ctgggacgat ccgcaagacg agcaccgcca
     2521 tgtctcgcag ctttatgccc tgcatcccgg caaccgcatc gcgccggcga ccgagccggc
     2581 gcttgccgcg gcggcgcgcg caacgctgaa agcgcgcggc gacggcggca ccggctggag
     2641 caaggcgtgg aagatcaatt tctgggcccg gctcggcgac ggcgatcacg cccattcga
//
LOCUS       DASWPU010000307         4816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_57597,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000307 DASWPU010000000
VERSION     DASWPU010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57597"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGC27_06530"
     CDS             complement(<1..263)
                     /locus_tag="VGC27_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282261.1"
                     /translation="MPSAKDRLYHLLKLAEEGSARRAALVDELADLLLDWPADCPEAM
                     RALVAALFEKTVRETGDDTRAKLAARLGGHNELPLDLVNEFYLA"
     gene            complement(328..1485)
                     /locus_tag="VGC27_06535"
     CDS             complement(328..1485)
                     /locus_tag="VGC27_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282262.1"
                     /translation="MTAPQIDRFATIEKAIGTLKPVEPVYCLFPQKFQIAAKRFLDGF
                     PGDAMYAVKANPVPQVLDLVHAAGIRHFDTASLGEVELVKGRFPDAICHFMAPVRLTG
                     HAKAAFEKYGVTDYVVDCDFELDKLIAETEHPEKLRIFVRMVTQLGGALLELSSKFGT
                     TPDEAARLLRRVKELGAAPCLTFHVGSQCLSSFSYGQAIEIARRAIDKSGLKIAALDI
                     GGGFPAQYNNNDVPPYHWYFDTIREALATLEHDGLPILCEPGRALIAEGMSLITQVVL
                     RKGDRLFINDGTYGSFDELTLPGWNADYPNRVFTVDAKNRALPLPGALRPFRVYGPTC
                     DTLDVLPRPQMLPEGIGPGDYIVFDTIGAYSAAVRTTFNGFYPDHWAMIGN"
     gene            complement(1920..2513)
                     /locus_tag="VGC27_06540"
     CDS             complement(1920..2513)
                     /locus_tag="VGC27_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY0282263.1"
                     /translation="MKSRILVPFLASLALCASAAMAQDTPAPAPGAAHQTHRHIGKEK
                     MGKHHKQMCTDLYARAVSRLSYVETKLALTDRQKPLFERWKKVKLDNAKARADKCAAL
                     KTTAMRASVVERAKLHEKMLKTKLANLQAELPSLEALVASLNDEQKHVLDRSRGAFGD
                     RMHGKHGKHGKHGTTGRMHGHHGGFGTNDAPPPPPAN"
     gene            complement(2657..3145)
                     /locus_tag="VGC27_06545"
     CDS             complement(2657..3145)
                     /locus_tag="VGC27_06545"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEY0282264.1"
                     /translation="MPLIKNGTYAEDSFAAVADGAPLPDGPAVVSLTRFLAERATLLT
                     RNAPLGVRLKSDESPAALGDDVQHLSLIVLEFPKFRDGRAFSWARLLRTRLGYRGEIR
                     AAGDFLYDQIAFMARVGIDAFEVSEKITPELFQRALGEMRFVYQPSADGRKTIRELRA
                     GS"
     gene            complement(3132..4790)
                     /locus_tag="VGC27_06550"
     CDS             complement(3132..4790)
                     /locus_tag="VGC27_06550"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY0282265.1"
                     /translation="MYRYDEFDAAFVRERVEQFRYQVERRLTGELNEDQFKPFRLMNG
                     LYLQLHAYMLRIAVPYGTLSARQLRMLAHIARKYDKGYGHFTTRQNFQLNWPKLKDVP
                     DILAEIATVEMHAIQTSGNVIRNVTADHFAGAAADEIEDPRPTAEIIRQWSSLHPEFT
                     FLPRKFKIAVSGAEHDRAALRFHDVAVQIVKTAAGETGTRIYVGGGMGRTPYINYVIA
                     DFVAKPDLLAFLEAVLRVYNQEGRRDNIYKARIKILAHTLGPDEMRKRVMREFAEIKR
                     AGTLQLPAEELARIAAYFAPPEFEALSDEMPAPADKDFADWAKTNLHPHRAPGYAIAT
                     ISLKPIGGVPGDASHQQLDALADLLDRFAIPEIRVSHEQNLVLPHVRKKDLPAIFARL
                     KELDLATPNAGLVTDIIACPGLDYCDLANARSIPVAQAIAERFADLDRQHDIGELKIK
                     ISGCVNACGHHHIGHIGILGVDKKGVEYYQLQLGGSAAEDASIGEIMGPAFGADEIVD
                     AVERVITAYTLLRVKGERFLDTYRRVGMEPFENMAYRDQLHAAH"
BASE COUNT          799 a         1432 c         1657 g          928 t
ORIGIN      
        1 gcaaggtaga attcgttgac cagatcgagc ggcagctcgt tatggccgcc cagtcgcgcc
       61 gccagcttgg cgcgcgtgtc gtcgccggtt tcgcgcaccg tcttttcgaa cagcgccgcc
      121 actagcgcgc gcatcgcctc ggggcaatcg gccggccagt cgagcaacag atcggccagc
      181 tcgtccacca gcgccgcgcg ccgggcggat ccttcttccg ccaacttcag cagatggtac
      241 agacggtcct tcgccgacgg catcgcttgc tcccttgccg ggcaagacta ggcggcggat
      301 gttaaggttt cgttggctat gaatctgtta attcccgatc atcgcccaat gatccgggta
      361 aaagccgttg aaggtggtgc gcacggccgc cgaataggcg ccaatcgtgt cgaagacgat
      421 gtaatcgccc ggcccgatgc cttcgggcag catctgcggg cgcggcagca cgtcgagggt
      481 gtcgcaggtc ggcccgtaga cccggaacgg cctgagggcg ccgggcagcg gcagggcgcg
      541 gttcttggcg tccaccgtga acacgcggtt ggggtaatcg gcgttccagc cgggcagggt
      601 cagttcgtcg aagctgccat aggtgccgtc gttgatgaac agccggtcgc ctttgcgcag
      661 caccacctgg gtgatcagcg acattccctc ggcgatcagc gcgcgccccg gctcgcacag
      721 gatgggaagg ccgtcatgct ccagcgtcgc cagggcttcg cggatggtgt cgaaatacca
      781 gtgatagggc ggcacgtcgt tgttgttgta ctgggcggga aagccgccgc cgatgtcgag
      841 cgcggcgatc ttgaggccgg atttgtcgat cgcgcgccgg gcgatctcga tcgcctggcc
      901 gtaggagaac gacgacaggc attgcgagcc gacgtggaag gtgaggcagg gcgccgcgcc
      961 gagttccttg acgcgccgga gcagccgcgc ggcttcgtcg ggggtggtcc cgaacttgct
     1021 ggagagttcg agcagcgcgc cgcccaattg cgtcaccatg cgcacgaaga tgcgcagctt
     1081 ttccgggtgc tcggtttcgg cgatcagctt gtcgagttcg aagtcgcaat cgacgacgta
     1141 atcggtgacg ccgtatttct cgaacgccgc cttggcgtgg ccggtcaggc gcaccggcgc
     1201 catgaagtgg cagatcgcgt cggggaagcg gcctttgacc agttcgactt cgcccagcga
     1261 ggcggtgtcg aaatggcgga tgccggcggc atggacgagg tcgagcacct gcggcaccgg
     1321 gttcgccttg accgcgtaca tcgcgtcgcc gggaaagccg tcgaggaaac gcttggccgc
     1381 gatctggaat ttctgcggga acaggcaata gacgggttcg accggcttga gcgtgccgat
     1441 ggctttctcg atggtggcga agcggtcgat ctgtggcgcg gtcatcggcg cggcctcaaa
     1501 agtgcgcgcg cttcttgggg caaggggggc ggagggtcaa gataaagata cagattaaag
     1561 agttacctcc cccttgtgag gagggcgata tcctgcccag gaaccgcttg tgaccgaacg
     1621 caaccaacta ctcacttggt catggcccgc ccttgtgcgg gccacccagg tgacgagggc
     1681 gcgttgcccg aagaatttca cggcgtctat tctttgatga ttttttgcag acttcaactg
     1741 gatggcccgc agtcgctggc catgacaccg aggttttgag tgccagcttg atgtccggcg
     1801 accctaagat ggaaacctct gcgcggccag tgccgtcaaa aaacttgcgg gttccggcgg
     1861 gggactcgga acccgcaagt gggctttcca aaggaggagg ggaaagccgg ttcgtggttt
     1921 tagttcgcgg gcggcggggg cggcgcgtcg ttggtgccga agccgccgtg atgaccgtgc
     1981 atacgtcccg tcgttccgtg cttgccgtgc ttaccgtgct tgccgtgcat ccggtcgccg
     2041 aacgcgccgc gcgaccggtc gaggacgtgc ttctgctcgt cgttcagcga agcgacaagg
     2101 gcttcgaggg agggcagctc ggcctggagg ttggcgagtt tggtcttcag catcttctcg
     2161 tgcagcttcg cccgctcgac gacggaggcg cgcatagcgg tcgtcttcag cgcggcgcat
     2221 ttgtcggcgc gcgccttggc gttgtccagc ttgaccttct tccagcgttc gaacagcggc
     2281 ttctgcctgt cggtgagcgc gagcttggtt tcgacgtagg acaggcggct gacggcgcgg
     2341 gcgtaaagat cggtgcacat ctgcttgtga tgtttgccca tcttttcctt gccgatatgc
     2401 cggtgggttt ggtgggcggc gccgggggcg ggcgccgggg tgtcttgtgc catcgccgcg
     2461 gaggcgcata gcgccagcga agcgaggaag ggaacgagaa tcctggactt catgggggta
     2521 acctcgggtt tgtttgaccc cattctaacg actggccccg cgcaatccgt ctgaatttga
     2581 gataattttt gtcgcgaatt gtcgagggtc cgcgccacga gcggcttttc cgccgttttg
     2641 gcaaggattc ggacggctat gaaccggccc gcagctcgcg aatggtctta cgcccgtcgg
     2701 cgctgggctg atagacgaag cgcatctcgc cgagcgcgcg ctgaaagagt tccggggtga
     2761 tcttctcgga aacctcgaac gcgtcgatgc cgacgcgcgc catgaaggcg atctggtcgt
     2821 agaggaagtc tccggcggcg cggatttcgc cgcgatagcc cagccgcgtg cgcagcagcc
     2881 gcgcccagga gaaggcgcgc ccgtcgcgga acttggggaa ctccagcacg atcagggaaa
     2941 gatgctgcac gtcgtcgccc agagcagccg gagactcgtc ggatttgagc cgcacgccga
     3001 gcggcgcatt gcgggttagc agcgtggcgc gctcggccag gaagcgcgtc agcgaaacca
     3061 cggccggccc gtcgggcagc ggcgcgccgt cggcgacggc ggcgaaggaa tcctcggcat
     3121 atgtcccgtt tttaatgagc ggcatggagc tgatcccgat aggccatgtt ctcgaacggc
     3181 tccatgccga cgcgccggta ggtgtcgagg aagcgttcgc ccttgacgcg cagcaaagtg
     3241 taggccgtga tcacgcgctc caccgcatcg acgatttcat cggcgccgaa agcggggccc
     3301 atgatctcgc cgatggaggc gtcctccgcg gcggagccgc cgagctggag ctgatagtac
     3361 tccacgccct tcttatcgac gccgagaatg ccgatgtgcc cgatgtgatg atggccgcag
     3421 gcgttgacgc agccggaaat cttgatcttg agttcgccga tgtcgtgctg gcggtcgagg
     3481 tcggcgaagc gctcggcgat cgcctgggcg acgggaatcg aacgcgcatt cgccaggtcg
     3541 caatagtcca ggcccgggca ggcgatgatg tcggtgacga gaccggcatt gggcgtggcc
     3601 aggtccagct ccttcagccg cgcgaagatc gccggcaggt cctttttgcg cacatgcggc
     3661 agcaccagat tctgttcgtg gctgacgcgg atttcaggaa tggcgaagcg gtcgagcaga
     3721 tcggccagcg cgtcgagctg ctggtggctg gcgtcgcccg gcacgccgcc gatgggcttg
     3781 agcgaaatcg tggcgatggc gtagcccggc gcgcgatgcg gatgaaggtt ggtcttcgcc
     3841 cagtccgcga aatccttatc cgccggcgcc ggcatctcgt cgctcaacgc ctcgaattcg
     3901 ggcggggcga aataggcggc gatgcgcgcc agttcctcgg ccggaagttg caacgtgccc
     3961 gcccttttga tctcggcgaa ttcgcgcatc acgcgcttgc gcatttcgtc gggtcccagc
     4021 gtgtgcgcca ggatcttgat gcgcgccttg tagatgttgt cgcggcggcc ctcctggtta
     4081 tagacgcgca agacggcttc gaggaaggcc agcaaatccg gcttggcgac gaaatcggcg
     4141 atcacgtagt tgatataggg cgtacgcccc atcccgccgc cgacataaat gcgggtgccg
     4201 gtttcgcccg ccgcggtctt cacgatctgc accgccacgt cgtggaacct gagcgccgcg
     4261 cggtcgtgct cggctccgct caccgcgatc ttgaatttgc gcggcaggaa ggtgaattcg
     4321 ggatgcaggc tcgaccattg gcggatgatc tcggcggtcg ggcgcgggtc ttcgatctcg
     4381 tcggcggcgg cgccggcgaa atgatcggcg gtgacgttgc ggatcacatt gccgctggtc
     4441 tggatcgcgt gcatttccac cgtggcgatc tcggccagga tgtcgggcac gtccttcagc
     4501 ttgggccagt tgagctggaa attctggcga gtggtgaaat ggccgtagcc cttgtcgtat
     4561 ttgcgcgcga tgtgggcgag catgcgaagc tgcctggccg acagcgtgcc gtaaggcacc
     4621 gcgatgcgca gcatgtaggc gtgcagttgc aagtacagcc cgttcatcag ccggaaaggc
     4681 ttgaactggt cttcgttcag ttcgcccgtg agccggcgct ccacctgata gcggaactgc
     4741 tcgacgcgct cgcgcacgaa ggcggcatcg aattcgtcat atcgatacat cgtcgtcgcc
     4801 ctttgcctcc cccctt
//
LOCUS       DASWPU010000308         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_94489,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000308 DASWPU010000000
VERSION     DASWPU010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94489"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1513)
                     /gene="aroA"
                     /locus_tag="VGC27_06555"
     CDS             complement(164..1513)
                     /gene="aroA"
                     /locus_tag="VGC27_06555"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY0282266.1"
                     /translation="MSDLAPSRPLKARRSAPLKGVAAVPGDKSISHRALILGALALGE
                     TRITGLLEAEDVLGTAEAVRAFGARAIRTGAGAYLVHGTGVGGWGEPENVLDFGNSGT
                     GSRLVMGAMATTPVTAVFTGDASLRRRPMGRVLEPLRLFGAEYAARAGGLMPVTLTGA
                     AQAICVEHMVAVASAQVKSALLLAALNAPGRSRISQATPTRDHTERMLAAFGAEISVE
                     PLADGGEVISLTGEAELKGTKVDVPRDPSSAAFAVVAGLIVPGSEISVPAVLLNPRRI
                     GLIETLKEMGAKIDVANLRESGGEKIGDLLVRASALKGMEVPPERAAAMIDEYPILAV
                     AAAFAEGPTVMRGLEELRFKESDRLAAIVAGLRAGGVSVQETEDGITVEGRGADGVPG
                     GGRIATRMDHRIAMSFLVMGLASQGPMSVDDASVIATSFPEFERLMRHLGAEFEAVS"
     gene            1647..2138
                     /locus_tag="VGC27_06560"
     CDS             1647..2138
                     /locus_tag="VGC27_06560"
                     /inference="COORDINATES: protein motif:HMM:NF021083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEY0282267.1"
                     /translation="MVKADLGTKRVCPSCGARFYDLTKRPIECPKCAFTFEPEAMYKQ
                     HRPRQPEPVPVAQPAAEVEEEEVEEEVEEAEEEAIVEPIPGEPVLETGDDDEEEEAVP
                     EEPEAPAVGISVVEGEDAAIEDIEDADVEEDDEDDGLLEAVEEDEDDVSGIIDADIEK
                     YER"
     gene            2176..2251
                     /locus_tag="VGC27_06565"
     tRNA            2176..2251
                     /locus_tag="VGC27_06565"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2209..2211,aa:Ala,seq:ggc)
BASE COUNT          430 a          786 c          740 g          340 t
ORIGIN      
        1 agatgttcgt ccgaatcttc gcaccatggg ttacgcacgg cgcatgaccg ccgccttctc
       61 ggtcgatgtg gcgaccaaaa ccgttgcgat acacggcgtg ttctatggcg ggcgagattt
      121 cgagcagttg ctccgcgacg gcgccgattg agagtgctga cctttaactc actgcctcga
      181 attcagctcc caaatgccgc atcaggcgct cgaattccgg aaagctggtg gcgatcaccg
      241 aggcgtcgtc cacgctcatc ggcccctgcg aggccaggcc catcaccagg aacgacatgg
      301 cgatacggtg atccatgcgg gtcgcgatcc gtccgccgcc cggaacgccg tcggcgcccc
      361 ggccttcgac cgtgatgccg tcttccgttt cttgcaccga gacgccgccg gcgcgcagtc
      421 cggcgacgat ggcggccagc cggtcgcttt ccttgaagcg caattcctcc agcccgcgca
      481 tgacggtcgg cccttcggcg aaggcggcgg cgaccgccag gatgggatat tcgtcgatca
      541 tcgccgccgc gcgctcgggc ggaacctcca tgcccttgag cgccgaggcg cgcaccaaga
      601 ggtcgccgat cttctcgccg ccgctttcgc gcaggttggc gacgtcgatc ttcgcgccca
      661 tctccttgag cgtctcgatc aggccgatac ggcgcggatt gagcagcacc gcgggaacgg
      721 aaatctcgga accgggcacg atcagccccg ccacgacggc aaaggcggcc gaggacggat
      781 cgcgcggaac gtccaccttc gtgcctttca gctccgcctc gcccgtcagc gaaatgacct
      841 cgccgccgtc cgccaaaggt tcgacggaaa tctcggcccc gaaggccgcc agcatgcgct
      901 cggtgtgatc gcgggtcggc gtcgcctgcg aaatccgcga acggccgggc gcgttcagcg
      961 ccgccagcag cagcgccgac ttcacctgcg ccgaggcgac cgcgaccata tgttccacgc
     1021 agatggcctg cgccgcgcct gtgagcgtaa ccggcatcag gccgcccgcc cgtgcggcgt
     1081 attccgcccc aaacagccgg agcggctcca gcacccggcc catcgggcgc cggcgcagcg
     1141 aggcgtcgcc ggtgaacacg gcggtgacgg gcgtcgtcgc catcgccccc atcaccaggc
     1201 gcgatccggt gccggaattg ccgaaatcga gcacattttc cggctcgccc cagcccccca
     1261 cccccgtacc gtggacaaga taggctccag ccccggtgcg gatcgccctg gcgccgaacg
     1321 cccgcaccgc ctcggcggtg cccagtacgt cctcggcctc aagcaggccc gtgattctgg
     1381 tctctcccag cgccagggcg cccaggatca aggcccggtg ggagatcgat ttgtcgcccg
     1441 gaacggccgc tacccccttc aaaggggccg aacggcgcgc tttcaacggg cgggatgggg
     1501 cgagatcgga catcgcaagg gcttccgtaa agggcaggga ttggctaacc gattgggccg
     1561 ccgggtcaag ggctttgaca ggcggttgca gccgtggcaa acgacccccc cttcggcaac
     1621 ggatcatcac gcgggataca ggtaaattgg tcaaagcgga tctcggaacg aaacgtgttt
     1681 gcccaagttg cggcgcgcgc ttctacgacc tcaccaaacg ccccatcgag tgcccgaaat
     1741 gtgcgttcac attcgagccc gaggccatgt acaaacagca ccgtccgcgg cagcccgagc
     1801 ctgtgcccgt cgcgcagccc gcggcggaag tcgaggaaga ggaagtcgag gaagaggtcg
     1861 aggaggccga agaagaggcc atcgtcgagc cgatcccggg tgagcccgtg ctcgaaaccg
     1921 gcgacgacga cgaggaagaa gaagccgtgc ccgaagagcc ggaagctccc gccgtcggca
     1981 tctccgtggt cgaaggcgaa gacgccgcga tcgaggatat cgaggacgcc gacgtcgagg
     2041 aagacgacga ggacgacggc ctgctcgaag cggtcgaaga agacgaagac gacgtcagcg
     2101 gcatcatcga cgcggacatc gaaaagtacg agcgctaata tcatccgttc ccggtcccca
     2161 aaccgggatg catatggggc catagctcag ctgggagagc gcctgcatgg catgcaggag
     2221 gtcagcggtt cgatcccgct tggctccacc agtcttcgcc gcgcagcggc ggagactgcc
     2281 acgtcgaagc ccgaag
//
LOCUS       DASWPU010000309        10079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_21315,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000309 DASWPU010000000
VERSION     DASWPU010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10079
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21315"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /gene="rplT"
                     /locus_tag="VGC27_06570"
     CDS             complement(<1..209)
                     /gene="rplT"
                     /locus_tag="VGC27_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY0282268.1"
                     /translation="MSRAPRAVPSHARRQRVFKAAKGFRGRRKNTITSANAAVDHSLQ
                     YNYIGRKLKKRNFRALWIQRINAACR"
     gene            complement(223..420)
                     /gene="rpmI"
                     /locus_tag="VGC27_06575"
     CDS             complement(223..420)
                     /gene="rpmI"
                     /locus_tag="VGC27_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY0282269.1"
                     /translation="MPKMKTKSGAKKRFKLTAKGKIKTGQTGKRHRLISNTPERTSTL
                     RGTSVLSESETQRVKRWMPYG"
     gene            698..1069
                     /locus_tag="VGC27_06580"
     CDS             698..1069
                     /locus_tag="VGC27_06580"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY0282270.1"
                     /translation="MKWSHVLALYLFWPAVLLVAWSELTPQPPHLETEIWDKALHFIA
                     YFSLAGLATVALDARRPALWAVLSLVILGGVLEILQGFTGRDPSLTDEIANALGAGLG
                     GCSGWLLMSLLQNKLLVPGRD"
     gene            1133..1540
                     /gene="mscL"
                     /locus_tag="VGC27_06585"
     CDS             1133..1540
                     /gene="mscL"
                     /locus_tag="VGC27_06585"
                     /inference="COORDINATES: protein motif:HMM:NF010557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY0282271.1"
                     /translation="MLEEFKKFAMRGSVVDLAVGVIVGAAFTSVVNSLVKDVITPPIG
                     WISGGIDFSNLFFVLKGGSYATLAEAAKAGAVTVNYGLFLNAVISFLIVAFVLFLVVR
                     QLNRLAVPKKAETATPEDIVLLREIRDLLKEQA"
     gene            1537..2076
                     /locus_tag="VGC27_06590"
     CDS             1537..2076
                     /locus_tag="VGC27_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282272.1"
                     /translation="MSLRRFFASSLVSAALAAAAGSAADSPAQAAEGFYAAYSTFHPS
                     GGIPDASGRAKYAPFISPALDKLLAEGNAAEIKFSKAHKASPPLIGGDLFTSMFEGAT
                     SYKVGACKLDGAQASCSVDLVYDDKKDQPIRWTDTVTLVKIKADWRVDDIVYGGNWEY
                     ANKGLLSTILRQAIADSGG"
     gene            2161..3576
                     /locus_tag="VGC27_06595"
     CDS             2161..3576
                     /locus_tag="VGC27_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282273.1"
                     /translation="MALSLPKSLSNARLIVLGAALAAFAVVLFTPAVFNDGDTYWHIA
                     AGRWMLDNQAVLRIDPFSFTFAGHPWQTHEWLAEIVMALAYVGAGWSGVALLFGAAAA
                     LTAGLLAHHLSRRLGGLALIVTLLLSLACVAGSLLARPHLLALPLLVLWTAGLLGARE
                     EERAPSWKLLPLMALWANLHSSFLIGLVLILPLALEAVQEKAHKHAARSWGVFAALAL
                     LAALATPHGIFGLLFPFKLMAMPSLQLIGEWAPTDFRIFQPLALAGAAALYVLLSRGA
                     TVKPLRLLILLGLLYLALIHVRHHMLIGIVGALILAEPLGRALQSPPIDAKPRHGAAA
                     LLFAVLLGALSALRLVLPLERGDGPTTPATALAQVLASLQHSPVLNDYAFGGYLIFND
                     VRPFIDSRAELYGEKFLARYARMVRPDKPALEAALAKYRVRWTIFAADNQAAAAMDAM
                     AGWHRLYADRWAVVHVRDETK"
     gene            3573..4016
                     /locus_tag="VGC27_06600"
     CDS             3573..4016
                     /locus_tag="VGC27_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0282274.1"
                     /translation="MSVKRDLYEELERQARGLLAGERDAVANAANLSALIWQFLPDLN
                     WAGFYFLKGRELVLGPFQGKPACVRIALGKGVCGTAAADLKTILVPDVHRFPGHIACD
                     AASNSELVAPLLADGFAVGVLDLDSPLFGRFDADDATGIEALARL"
     gene            complement(4396..6897)
                     /locus_tag="VGC27_06605"
     CDS             complement(4396..6897)
                     /locus_tag="VGC27_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0282275.1"
                     /translation="MFAITGSVSYDATDTKTKEYQAYNRAPWVITTTGVSGYTGVGSL
                     NATNGETNLPGGASYISPQYAYFSDIYAKNKTIGATLGGTWNVSDSIQTSLNWFYSHI
                     SDKQTNFSNKVGFNGSGATSNTPGSGMPGINLDEGYTIDTNGVVETAHFYLQGAETAT
                     LFQKTGSDAHNIQWHTSFDDGGPITATLDVSWAGAKSSLKAAQQDIEHGYYTAQGQST
                     SAAPTAPGCNNFSPSCVDGNPAIPLIWNNGGTSGLPTAEYLAPYADVLSNPNYVLFKS
                     AWAWANHSKQQQGAIRGAAVYKPAFLTGVDGGIEAGFRLARRDVWQDFGRYLIDGRDV
                     NGNYISNCCYAEGNGPGLYYQDPGYAAIPYDTAVSNPDLALTVRSFIFGNMIVKDPAA
                     GGMTDPETFLNTIWNQAAFVPTNTPSAVIVGGVAVPCTTVPAPAACAVPAQTAVPNNS
                     QALFIDTLSSFKVHETTKAFYLMSDLGTKDNGFHLNAGVRVVLTHQEISGASAAPTVY
                     SYGTATWNGVNSNNIPFTSGKDIVDILPSFNLAIYPTDDQIIRFSGARVVSPADLFNL
                     GVGRYYNFTRETGGRVNVNPSSPSFGTQEGYRFANGTSGNPDLDPYRANQFNLSWEYY
                     FAPEALASVGTFYKQVESFEVNQPINTLVVDDFGGSEGPVNMAVNAGHGSIYGFELVG
                     QYVYESGFGFAANYTYTQSQSAIVTAFSDHLPIPDVSAHAATGTIFYSNYGLDARLSY
                     SWRSKAVNGGIGGATFSPVDSATGSSIAYGVFTAPYGQLDAQVTYNVNDNLALIVSAQ
                     NLTDEAYHTYLQFPNQPFIYDASGTRYFFGVRFRM"
     gene            complement(6810..7436)
                     /locus_tag="VGC27_06610"
     CDS             complement(6810..7436)
                     /locus_tag="VGC27_06610"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0282276.1"
                     /translation="MNRNLKFCTQTAVAALLVGVGLTPAQAQTEQGQIETVIVTGFRA
                     SLANALEAKQKSNLIMESVTAEDIGKMPDQNIAESLQRLPGIQIARDQGKGTRVLING
                     LRQNLTTLNGDVFLTGKEFYVSGEAANGGAGANAQYASLEGIPSEEIGGIDIYKTPNA
                     SLSEGGMGGIINLKTRNPPTCSPSRAASRTTPRIPRPKSTKPTTALLG"
     gene            complement(8025..8699)
                     /locus_tag="VGC27_06615"
     CDS             complement(8025..8699)
                     /locus_tag="VGC27_06615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0282277.1"
                     /translation="MHIQNIFLFDLDGTLVDSVYQHVLSWLEALDQEGVELSVWRIHR
                     KIGMSGGLFADQLLRETGLEITPELLDRLRRNHAEAYKRNATRVRPLPGAIELLATLT
                     EAGTPWAIATSGRMETAAPNIAALGIDPMKIPVVTRDQVKYAKPDPDLFLAAAGRLGV
                     PIENAVVVGDSIWDMLASLRCRALGVGVLSGGYGQDELERSGAVRVYEDPLDLLKHLD
                     EVGGRH"
     gene            complement(8815..8891)
                     /locus_tag="VGC27_06620"
     tRNA            complement(8815..8891)
                     /locus_tag="VGC27_06620"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8855..8857),aa:Arg,seq:ccg)
     gene            complement(9069..>10079)
                     /locus_tag="VGC27_06625"
     CDS             complement(9069..>10079)
                     /locus_tag="VGC27_06625"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0282278.1"
                     /translation="DEHVLFDALGIDLGLASVWLAGAGARWFEIARSRNRLRLAFADA
                     LPARTIDQIARNPQLMKLDGESRTVTYLACGVRGFAELAASFRGDPAAFTRLMQRVLE
                     PLMNEALDHGGTVAKLATEGFTAFWNAPLDDPEHAIHACEAANAMMEVIARTNEVITH
                     ERRIDGVALSPVEIGIGISTGPAIAGGFKAHGRTAYAVNGDCAVLAARIQQISGQYGP
                     AVIVSEDTRKSSERGFAFLEVDFIALGSHDEPVKLYAMLGNPVMRASPKFRALATFHD
                     HIFQSLRAQQWQKARELIEQCRKLSGASQKLYDLHLTRIAYFEDNPPGEDWDGAFRPI
                     LN"
BASE COUNT         1753 a         3045 c         3195 g         2086 t
ORIGIN      
        1 cggcacgccg cgttgatgcg ctggatccaa agcgcgcgga aattgcgctt cttcagcttg
       61 cggccgatgt aattgtactg cagcgaatga tcgaccgcgg cattggccga ggtgatcgtg
      121 ttcttgcggc gtccgcggaa gcctttggcg gccttgaaaa cgcgctggcg gcgggcgtgg
      181 gacggtacgg cgcggggtgc tctcgacatg gttttcgctc cctcagccgt acggcatcca
      241 gcgcttgacg cgctgggttt cggattcgga aagcaccgag gtgccgcgca gagtgctggt
      301 acgttcgggc gtgttcgaga tcaggcggtg gcgcttgccg gtctggcccg tcttgatctt
      361 gcccttggcg gttaatttga agcgcttttt ggcgcccgat ttggtcttca tcttgggcat
      421 ttcggtctcc tttttgagtc tgggtggccg gattggcgtc cggccatgga ccatttggag
      481 ccgccacggc atgcccttgc gccggtcggc tcaactattt gccggtgcat gatccgcaaa
      541 aacgggaacc ggttttgcgg atcatgcact gggagggcgc gtcttacacg gaagccgtat
      601 ttatttgcaa ggtttaacgc gatttcgcca ctttccatgc caatatgctg cggcgcatct
      661 caaaagacgt acattcgcgg tgtggagaca aagactgatg aaatggagcc atgtgctggc
      721 cctttacctg ttctggccgg ccgttctgct ggtggcatgg agcgaactga ccccccagcc
      781 gccccatctg gagacggaga tttgggacaa ggccttgcat tttatagctt atttcagctt
      841 ggctgggctg gcgaccgtag cccttgacgc ccgccggccg gcgctttggg cggttctgtc
      901 gctggtaatc ctgggcgggg tgctcgaaat cctgcaaggc ttcacggggc gcgatcccag
      961 ccttaccgac gaaatcgcca acgccctggg cgccggccta ggcggctgtt ccggctggct
     1021 gctgatgagc ctgctgcaga acaagctgct ggtccctggg cgcgactgaa acgccgcggt
     1081 aaagtggcgc gcgccggatg tgacgaagcc tctttgccag gatgagccag ccatgctcga
     1141 agaattcaag aagttcgcca tgcgcggcag cgtcgtcgat cttgcggtcg gcgtgatcgt
     1201 cggcgcggcg ttcaccagcg tcgtcaattc gctggtcaag gatgtgatca ccccgcccat
     1261 cggctggatc agcgggggca tcgatttttc gaatctgttt tttgtgctga aaggcggcag
     1321 ctatgcgact ttggccgagg ctgcaaaggc gggcgccgtc accgtcaatt acggactgtt
     1381 tctgaacgcg gtcatcagtt ttctgatcgt cgcctttgtg ctgtttctgg tcgtgcgcca
     1441 gctcaaccgg ctggccgtac cgaaaaaggc cgagaccgca acccccgaag acatcgttct
     1501 gctgcgcgaa atccgcgatt tgctgaagga gcaagcatga gcctgcggcg ctttttcgcg
     1561 tccagccttg tatcggctgc gcttgcggcc gccgccggtt ctgccgccga ttcgccggcc
     1621 caggcggcgg aaggtttcta tgcggcctat tccaccttcc atccctccgg cggcattcct
     1681 gacgcgtccg gccgcgccaa atacgcgccg ttcatttccc ctgccctcga caagctgctg
     1741 gctgaaggca acgccgccga gatcaagttc agcaaggcgc acaaggcctc gccgccgctg
     1801 atcgggggcg atttgttcac ctcgatgttc gagggcgcca cttcgtacaa ggtcggcgcc
     1861 tgtaaactgg acggcgcgca ggcaagctgc agtgtcgatc tcgtctatga cgacaagaag
     1921 gaccagccga tccgctggac cgacacggtc actctggtca agattaaggc ggactggcga
     1981 gtcgacgaca tcgtctatgg cggcaactgg gaatacgcca acaagggcct gctcagcacg
     2041 attttgcgcc aggcgatcgc cgattccggc gggtaagctg ggcggtttcg gcggggattc
     2101 ttgattctgc gcagccaccc gcgtaggcag gaagccgcct tttcttaagc ggaccattgc
     2161 ttggcgctgt ctttgccgaa atcgctttcg aacgcgcgcc tcatcgttct aggggcggcg
     2221 ctggcggctt ttgcagtggt gctgttcacg cccgcagtct tcaacgacgg cgacacctat
     2281 tggcatatcg ccgccggccg ctggatgctc gacaaccaag ccgtcctgcg catcgatccg
     2341 ttctccttta ccttcgccgg ccacccctgg cagacgcacg aatggctcgc cgaaatcgtc
     2401 atggcgctgg cgtatgtcgg cgcgggctgg agcggtgtgg cgcttctgtt cggcgcggcg
     2461 gcggcgctga ccgcgggact cttggcccac catctgtcgc ggcggctggg cgggctggcg
     2521 ctgatcgtca ccttgctgct gtcgctcgcc tgcgtcgccg gaagcctgct ggcgcggccg
     2581 catcttctgg cgctgccgct gctggtgctg tggactgccg gactcctcgg cgcccgcgag
     2641 gaggagcgcg cgccgtcgtg gaagcttctg ccgctgatgg cgctgtgggc caatctacat
     2701 tcgagctttc tgatcgggct ggtgctgatc ctgcccttgg cgctggaagc cgtgcaggag
     2761 aaggcgcaca agcacgccgc ccggagctgg ggcgtcttcg ccgctctggc gctcttggcc
     2821 gcgttggcca cgccgcacgg gattttcgga ctgctcttcc cgttcaagct catggcgatg
     2881 ccgagcctac aattgatcgg cgaatgggcg ccgacggatt tccgtatctt ccagccgctg
     2941 gcgctggccg gggccgcggc gctttacgtt ctgttgtcgc gcggcgcgac ggtcaaaccg
     3001 ctgcgtcttc tgattctgct gggcctgctc tatctggcgc tgatccatgt gcgccaccac
     3061 atgctgattg gcattgtcgg agcgctgata ctcgccgaac cgctcggccg cgccttgcag
     3121 tctcccccga tcgacgccaa gcctcgtcac ggagccgctg cgcttctctt tgccgtgctt
     3181 ctcggcgcgc tgtcggcgct gcgcctggtg ctgccgctcg aacgcggcga cggccccacg
     3241 accccggcca ccgcactcgc ccaggtgctg gcttcgctgc agcatagccc cgtgctcaac
     3301 gattacgcct ttggcggcta tttgatcttc aacgatgtgc ggcccttcat cgacagccgc
     3361 gccgagcttt acggcgagaa attcctcgcc cgttacgccc ggatggtgcg ccccgacaag
     3421 ccggcgttgg aggccgcgct ggccaaatat cgcgtgcgct ggacgatttt cgccgccgac
     3481 aatcaagccg ccgccgccat ggacgcgatg gccggctggc atagactata tgccgaccgc
     3541 tgggcggtgg ttcatgtgag agacgagacg aaatgagtgt gaagcgcgat ctctatgaag
     3601 agctggagcg ccaggcgcgc gggctgcttg cgggcgaacg cgacgccgtc gccaatgccg
     3661 ccaatctttc ggcgctgatc tggcagtttc ttcccgatct caactgggcc ggtttctatt
     3721 tcctgaaagg gcgcgaactg gtgctcggcc cgttccaggg caagcccgcc tgcgtgcgca
     3781 tcgcgctggg caaaggcgta tgcgggacgg cggcggcaga cctcaaaacc atcttggtgc
     3841 ccgacgtgca tcgcttcccc ggacacatcg cttgcgatgc ggcttccaat tccgaactcg
     3901 tggcgccgct cctcgccgat ggctttgccg tgggcgtgct cgatctcgac agccctctgt
     3961 tcggacgctt tgatgcggat gacgcaaccg ggatcgaggc tctcgcccgg ctctaggtcg
     4021 aggccagcga tcggttttga caggtggcgc gaccgctgtg acccgaaaaa agggcggctt
     4081 gtgctggcag gccgcccagt tcatttgcag gggagaataa cgggtgcagg tcccgtcaag
     4141 gccgtgaatc agaatttcgt gccgcgattc acgcaaaaat cacacgacgg gacggagcgg
     4201 gagacattgg agagggacac accgtaccga ttgccagact tgtcactgaa cacgataggc
     4261 cggcccgccg tgtggtaacg catgctacga tccattctct gcttgaaatg acctttcgag
     4321 ggaggagaga agcggcagcc gtttgaccgc cgcttcccag tgttgccggg gaaattgagc
     4381 ccccgttgcg gaaggttaca tccggaacct cacgccaaag aagtaccgag tgccgctggc
     4441 gtcatagatg aacggctggt tcgggaactg cagataggtg tgataagcct cgtcggtcag
     4501 gttctgcgcc gacacgatca aggccaggtt gtcgttgacg ttgtaggtga cctgggcgtc
     4561 gagctggcca taaggcgccg tgaagacacc gtaggcgatc gaacttcccg tggccgagtc
     4621 gaccggcgag aacgtggccc cgccgatacc gccgttcacg gccttgctgc gccacgagta
     4681 cgacagacgc gcgtcgagcc cgtagttcga gtagaagatc gtgccggttg cagcatgagc
     4741 cgacacgtcg gggatcggca agtggtcgct gaacgcggtg actatggccg actgggactg
     4801 cgtgtaggtg taattggccg cgaagccgaa gccactttcg tacacatact gccccaccag
     4861 ctcgaagccg tagatcgagc catggccggc gttcaccgcc atgttgaccg ggccttccga
     4921 gccgccgaag tcgtccacga ccaacgtgtt gatcggctgg ttgacttcga aactctcgac
     4981 ctgcttgtag aacgtgccga cgctggccaa cgcttccggt gcgaaataat actcccacga
     5041 caggttgaac tggttcgcgc gatacggatc gaggtccgga ttgcccgagg tgccgtttgc
     5101 gaacctgtag ccctcctgcg tgccgaatga gggactactg ggattcacgt tgacccggcc
     5161 gccggtttcg cgcgtgaagt tgtagtacct gccgacgcca aggttgaaca gatccgccgg
     5221 tgacaccacg cgagccccgc taaagcggat gatctgatcg tccgtcgggt agatcgcgag
     5281 gttgaacgac ggcaggatat ccacgatgtc cttgcctgac gtgaacggga tgttgttgct
     5341 gttcacgccg ttccaagtgg cggtgccgta ggaatacacg gtcggagccg ccgaagcgcc
     5401 gctgatctcc tggtgcgtga gaacgacgcg aacaccggca ttgagatgga agccgttgtc
     5461 cttcgtgccc aggtcggaca tcagatagaa ggccttggtg gtctcatgca ccttgaacga
     5521 gctcaaggtg tcgatgaaga gcgcttgcga gttgttgggc accgcggtct gagccgggac
     5581 tgcacacgca gccggtgcgg gcacagttgt acacggaacg gcaacaccgc ccacaataac
     5641 ggcggacggt gtgttggtcg gcacgaacgc ggcctggttc cagatcgtgt tgaggaacgt
     5701 ctccgggtcg gtcatgccgc cggcggccgg gtctttcacg atcatgttgc cgaatatgaa
     5761 gctgcgaacg gtaagcgcca aatccggatt ggacaccgcc gtatcgtacg gaatggccgc
     5821 gtagccagga tcctggtagt agagtccggg cccgttgccc tcagcgtaac agcagttgga
     5881 gatataattc ccgtttacat cccggccgtc gatcaggtag cggccgaagt cctgccaaac
     5941 gtcgcgccga gccaggcgga aaccggcctc gatgccgccg tcgacgccag tcaggaaggc
     6001 aggcttgtaa accgcggcgc ctctgatcgc accctgctgc tgtttggaat ggttcgccca
     6061 ggcccaagct gacttgaaca gcacatagtt cggattcgac aacacgtccg cgtaaggcgc
     6121 gagatactcg gcggtgggca ggccggacgt gccgccattg ttccaaatca gcgggatcgc
     6181 cggattgccg tcgacgcagc tgggcgagaa gttgttgcag cccggagccg tcggcgcagc
     6241 cgaggtcgat tgaccttgtg ccgtataata gccgtgctcg atatcttgct gggcggcttt
     6301 caggctggat ttcgcacccg cccaggagac atccaacgtg gccgtgatcg ggccgccatc
     6361 atcgaagctc gtgtgccact ggatgttgtg ggcgtccgag ccggtcttct ggaacagggt
     6421 cgccgtttcc gcaccttgca aatagaagtg ggccgtctcg acgacgccgt ttgtgtcgat
     6481 cgtatagcct tcatcgagat tgatgcccgg catgccgctt cccggcgtgt tcgaggtcgc
     6541 gcccgatccg ttgaaaccga ccttgttcga gaagttcgtc tgcttgtccg agatgtgcga
     6601 gtagaaccag ttgaggcttg tttggatcga atccgagacg ttccaggttc cgccgagcgt
     6661 cgcaccgatg gtcttgttct tggcgtagat atcgctgaag tacgcatatt gcgggctgat
     6721 gtacgacgcg ccgcccggaa ggttggtttc gccattggtc gcgttgagcg aacccacgcc
     6781 cgtgtagccg ctgacgcccg tggtcgtgat cacccaagga gcgcggttgt aggcttggta
     6841 ctctttggtc ttggtatccg tggcgtcgta cgagacgctg cccgtgatgg cgaacacgtt
     6901 ggcggattgc gtgtcttgag gttgatgatg ccgcccatgc cgccttccga cagcgacgcg
     6961 ttcggcgttt tgtagatgtc gatgccgccg atttcttcgc tcgggatgcc ctccagcgag
     7021 gcgtattgcg cgttggcgcc ggcgccgccg ttcgcggctt cgccggacac atagaattcc
     7081 ttgcccgtca ggaagacatc gccgttcagc gtggtcaggt tctgacgcag gccgttgatc
     7141 aggacgcgcg tgcccttgcc ctggtcgcgg gcgatctgaa tgcccggcag gcgctgcagc
     7201 gattccgcga tgttctgatc cggcatcttg ccaatgtctt cggcagtgac ggactccatg
     7261 atcagattcg atttctgttt cgcctcaaga gcatttgcca gcgatgcacg gaagcccgtg
     7321 acgatcaccg tttcgatctg cccttgttcg gtctgcgctt gtgcaggcgt gaggcccact
     7381 ccgacaagga gtgcggcgac cgccgtctgc gtgcaaaact tcaagttgcg attcatgtct
     7441 ggttccctct cctgtttgct aagctccatc cacgcgctgg agacctcccc cgtgcgagcg
     7501 ggactagctg cagatagatt atgctgaggg gtaagcgctt accaacgaag aagcatcaac
     7561 tcgcgctatg gtagcgctaa cagttgatag acgtgactaa ttcgccacag ttaacaatac
     7621 acgccttaac gacggcgcgg tggaaggaat ttgtaagtat agaagccgca ctacgagtgt
     7681 atatgttatt gttataacat ttggccgtat ccactcgata catcgaaacc atttccaaga
     7741 tcaattcctt actttaggta aatatattgc aaagggtaaa atcggcgtcc ggcggagcgg
     7801 ccgctttacg ccgtcagttc ggctcgcggc gccgttcggc ccaagcttct agagcggcgg
     7861 aaaccgggag tccgaggcga tggaaggtga ctgtccggcc gcccttagag cttgatccga
     7921 tttaattgaa tcggatcaag ctctagattc cttgattttc gcgcaatctg acggcaaacc
     7981 gcgtcggcac gctgctgcta ccgaccactt tgccggattg cgcgctagtg ccgcccgccg
     8041 acttcgtcca gatgcttcag cagatcaagc gggtcttcgt acacccgcac ggcgccgctg
     8101 cgttccagtt cgtcctgtcc atagccgccg gagagcacac ccacgccaag cgcgcggcag
     8161 cgcagcgatg ccagcatgtc ccagatgctg tcgccgacca ccaccgcatt ttcgatcggc
     8221 acgccgaggc gtccggcggc cgccagaaac aggtccgggt ccggcttggc gtatttgacc
     8281 tgatcgcgcg tcaccaccgg gatcttcata gggtcgatgc ccaaggcggc gatgttgggc
     8341 gcggccgttt ccatgcgtcc gctggtggcg atcgcccagg gggtacccgc ctcagtcaga
     8401 gtggcgagca gctctatcgc tccgggaagc ggtcttaccc gcgtggcgtt gcgtttgtag
     8461 gcctcggcat ggttccggcg cagcctgtcg agcagttccg gcgtgatctc taggcccgtc
     8521 tcgcgcagca gctggtcggc gaacagtccg ccgctcattc cgatcttgcg gtgaatgcgc
     8581 cagaccgaaa gctcgacgcc ttcctggtcc agggcctcca gccaggacag gacgtgctga
     8641 taaacgctgt ccaccagcgt gccgtcgaga tcgaacagaa agatgttttg aatgtgcatt
     8701 gccgaaatct cctaacgggg cggcttgccg accgaagttg cacgaacgga tgaaaaaccc
     8761 tcctccgctc tgatgagctt cagaggtcaa cctccgctgc caagccgcgc aggttggcgc
     8821 acccgacagg attcgaacct gtgacccctg ccttcggagg gcagtactct atccagctga
     8881 gctacgggtg cgagccggaa gagctataga caaggaacgg gccgatcgca acgaaccgct
     8941 gccgggccgc gtgcgtccgc cgccttttgt ccgccgccgc cctgattttt ttgcctttcg
     9001 ctagtgtccc gattccgaaa ttcgcccgca gccgatatcg agatggtgcg aatttcgaaa
     9061 ccacgacact agttcaaaat cggcctgaag gcgccgtccc aatcctcgcc cggcggattg
     9121 tcctcgaaat aggcgatgcg cgtgaggtgg agatcgtaga gcttctggct ggcgcccgaa
     9181 agcttgcggc actgttcgat cagttcgcgc gccttctgcc attgctgggc gcgcaacgat
     9241 tggaagatgt ggtcgtggaa ggtggcgagc gcgcggaatt tcgggctggc gcgcatcacc
     9301 ggattgccga gcatggcgta aagcttgacc ggttcgtcgt ggctgccgag cgcgatgaag
     9361 tcgacttcca aaaaggcgaa gccgcgttct gaggatttgc gcgtatcctc gctgacgatc
     9421 accgccggac cgtattggcc ggaaatctgc tggatgcggg cggcgagcac ggcgcaatcc
     9481 ccgttcaccg cataggcggt gcggccatgg gccttgaacc cgcccgcgat ggccgggccg
     9541 gtcgaaatgc cgatgccgat ttcgaccggc gacagcgcca ctccgtcgat gcgccgttcg
     9601 tgggtgatca cttcgttggt gcgtgcgatg acttccatca tggcgttcgc cgcttcgcag
     9661 gcatggatgg catgttcggg atcgtcgagc ggagcgttcc agaacgcggt gaagccctcg
     9721 gtggccagtt tggcgacggt gccgccatgg tccagcgcct cgttcatcag cggctccagc
     9781 acgcgctgca tcagccgggt gaaggcagca ggatcgccgc ggaaggaagc ggcaagctcg
     9841 gcgaaaccgc gcacgccgca ggccagatag gtcaccgtcc ggctttcgcc gtcgagcttc
     9901 atcagctgcg gattgcgcgc aatctggtcg atggtgcgcg cgggcagggc gtcggcgaag
     9961 gcgagcctga gcctgttgcg cgagcgggcg atttcgaacc agcgcgcgcc ggcgcccgcc
    10021 agccataccg aagccaatcc gagatcgatg cccagcgcgt cgaacagaac atgctcatc
//
LOCUS       DASWPU010000310         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_88090,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000310 DASWPU010000000
VERSION     DASWPU010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88090"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VGC27_06630"
     CDS             <1..206
                     /locus_tag="VGC27_06630"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY0282279.1"
                     /translation="LGAPLKGAMLIGNGPTDLRRVSMIGNDLQLDTGIGTCGKNGQSV
                     PVGVGQPTLRLDGLTVGGTAQAA"
     gene            203..550
                     /locus_tag="VGC27_06635"
     CDS             203..550
                     /locus_tag="VGC27_06635"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEY0282280.1"
                     /translation="MTDLIFKICHRAEWATAEREGIYAGSAADREDHFIHFSTAEQVM
                     GTLERHYASAGELVLVAVDPQPLGAILKYEPSRGGALFPHLYGTLPLTFVKWTAPIRR
                     GAGGAFLLPRACV"
     gene            620..1477
                     /locus_tag="VGC27_06640"
     CDS             620..1477
                     /locus_tag="VGC27_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0282281.1"
                     /translation="MQAIKGKTHDLGDGFEVSRVLPQIARRTVGPFVFFDYFGPVDFP
                     PGKGIDVRPHPHIGLATVTYLFEGSQVHRDSIGSVKSIEPGDVNWMTAGRGIAHSERT
                     GPEVRATGHRMHGIQSWVGLPQADEECAPSFQSIPAKDLPAGEKDGVRLRVAAGSAYG
                     LTSPVRVFSDIFYIDARLDAGAALPLPDDHIERAAFVVNGDVECSGSHFSRGDMILFD
                     PGEVATLAAPAAAHVMLLGGAPLDGPRHVWWNFVSSDRARIERAKAEWKSGGFGTIPG
                     ETEFIPLPE"
     gene            1590..>1787
                     /locus_tag="VGC27_06645"
     CDS             1590..>1787
                     /locus_tag="VGC27_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0282282.1"
                     /translation="MRYRQLGNTGMFVSELCLGTMTFGGQGSFFAHVGKLGQKEVTDI
                     VALALEAGVNFIDTADMYSFGV"
BASE COUNT          334 a          564 c          569 g          320 t
ORIGIN      
        1 agctcggcgc gccgctcaag ggcgccatgc tgatcggcaa cggcccgacc gatcttcggc
       61 gcgtgagcat gattggaaac gaccttcagc tcgacaccgg catcggcact tgcggcaaga
      121 acggacaatc ggtgccggtg ggcgtgggcc agccgacgct gcgcctcgac ggattgacgg
      181 tgggtggaac ggcgcaagcg gcgtgacgga cctcatcttc aaaatctgcc accgtgcaga
      241 atgggcgacc gccgagcgcg agggcatcta tgccggatcg gcggcggatc gcgaagatca
      301 cttcatccat ttctcgaccg ccgagcaggt gatgggcacg ctggaacgcc attatgcaag
      361 cgcgggcgaa ctggtgctgg tggcggtcga tccgcaaccg ctcggcgcta tactgaaata
      421 cgagccttcg cgcggcggcg cgctgtttcc gcatctctac ggaaccttgc cgctgacctt
      481 cgtgaaatgg acggcgccca tccggcgcgg cgccggcggc gcctttctgc tgccgcgcgc
      541 ttgcgtatga ccacaggggc catagggtag aaatcgtcac ctccggccat taggtttacc
      601 gcacagtgcg agatggacga tgcaagcaat caaaggcaag acgcacgatc tcggcgacgg
      661 cttcgaagta agccgggtcc tgccccagat cgcccggcgt acggtcgggc cgttcgtgtt
      721 cttcgattat ttcggcccgg tcgattttcc gccgggcaaa ggcatcgacg tgcggccgca
      781 tccgcatatc ggcctggcca cggtgacgta tctgtttgaa ggttcgcaag ttcatcgcga
      841 cagcatcggt tccgtcaagt cgatcgaacc gggggacgtg aactggatga cggcggggcg
      901 cggcatcgcc cattccgaac gcaccggccc ggaggttcgc gcgaccggcc atcggatgca
      961 cggcatccaa agctgggtcg ggcttccgca agccgatgag gaatgcgcgc cttccttcca
     1021 gagcattccg gcgaaagacc tgccggccgg cgaaaaagac ggcgtcagat tgcgtgtggc
     1081 cgccggcagc gcctatggcc tgacgtcgcc cgtgcgcgtc ttctccgata tcttctatat
     1141 cgacgcacgg ctcgacgccg gcgccgcact gcccctgccc gacgaccaca tcgaacgcgc
     1201 ggccttcgtc gtgaacggag acgtcgaatg cagcggatcg cacttttcgc gcggagacat
     1261 gatcctgttc gacccgggcg aagttgcaac cctcgccgcc ccggcagcgg cgcatgtcat
     1321 gctcctgggc ggcgcaccgc tcgacggacc gcgtcacgtc tggtggaatt tcgtgtcgag
     1381 cgatcgggcc cggatcgaac gcgccaaggc ggagtggaag agcggcggat ttggaaccat
     1441 cccgggcgag acggaattca tcccgctgcc cgaataacaa agaacccctg cgatgataga
     1501 caggaacatt tgatggctgc ttcgggttgg caagcgcgcc cgccagccgc taaagtattt
     1561 tcaaccccaa cctctccgga gacgtgtcca tgcgttacag gcaactcggc aataccggca
     1621 tgttcgtgtc ggaactttgc ctcggtacga tgacgttcgg cggccagggt tcgttcttcg
     1681 cgcatgtcgg caagctcgga caaaaggaag tcacggacat cgtcgcgctc gcgctcgaag
     1741 ccggcgtcaa tttcatcgat accgccgaca tgtattcctt cggcgtg
//
LOCUS       DASWPU010000311         9923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_45701,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000311 DASWPU010000000
VERSION     DASWPU010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45701"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VGC27_06650"
     CDS             complement(<1..396)
                     /locus_tag="VGC27_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0282283.1"
                     /translation="MRAAPSSVPTFAMAVACGIAVANIYYNQPMLALIERAFPGSATA
                     SLIPTATQLGFAAGLFLLVPLGDLLERRRVIVAQFLILGLALAAAAVAPTAPTLVAAS
                     LLVGAASSVAQQIVPFAATLAAPEKRGRTI"
     gene            499..1251
                     /locus_tag="VGC27_06655"
     CDS             499..1251
                     /locus_tag="VGC27_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282284.1"
                     /translation="MRPNGGSDIRQEFFLAGRKAAEENGAGVIGSLLLHGLAALLVLT
                     VIVKSAPQTAQKLLPFVAVDLVRLGMETESPPEARQALVPQQRAARRQEAASPTDEAV
                     GPNRTKPPLDTLEAKLRGLARLRQTQNNPNVAVGQGVSNVDASNGAPGDRATYSVRDY
                     VRAQVLRRWSLNLTKLAGHSFAVTIRVTMKRDGTILSAEIVERTNDPLYRDLALSARN
                     AVILASPIALPPGDLPATMRFTLDLDPKDARH"
     gene            complement(1252..2157)
                     /locus_tag="VGC27_06660"
     CDS             complement(1252..2157)
                     /locus_tag="VGC27_06660"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY0282285.1"
                     /translation="MNWRHRFSSVLLFAAAFASAASASAQERQEREFVECAGCPLMVG
                     IPAGKFVMGSPASEPGRFDYEGPQHVVSIKAFALGKYNVTSEEFLLFLKETGYQPEPC
                     NALLDMRWRSLGGGYAYPPYDEEPRLWPASCLDWKDAEAYIAWLNKKVRLEHPAITKN
                     PYRLPSEAEWEYAARAGTTTARWWGAEIGSGNANCNGCGSKWDNRVLAEVNSFAPNPF
                     GLYGMLGNVWQWTADCWHRSYVGAPNDGRPWTEKGCAKHVLRGGSWNNLPVFVRSAAR
                     SSGARSGGEYDYSSLAGFRVARDLP"
     gene            2686..3342
                     /locus_tag="VGC27_06665"
     CDS             2686..3342
                     /locus_tag="VGC27_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0282286.1"
                     /translation="MLTRRQTNAGILASAAFAVTSAVAVGQESKLRTLPPPRSKGGMP
                     LTEALLLRRSTRDYSERPIPPQVLSDLLWSAFGVNRPNGERTAPYWRHIMVTDIYLAM
                     ADGVWLYEPKAHALLPHSGEDIRALTGAQKFATTAPLNLVYVAHGERMTDVSLEDRRL
                     FASVNTGFIGQNVYLFCASEGLATVFRGSVDYPKLAQALKLPEQQFVTFAQTVGYPKG
                     "
     gene            complement(3434..4816)
                     /locus_tag="VGC27_06670"
     CDS             complement(3434..4816)
                     /locus_tag="VGC27_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0282287.1"
                     /translation="MAYQSFNPATGKLLKKFEELTDKELETKLAAAAACYEIWRHKTY
                     AERAVIVAKAAELLHAKAAEFARTMTLEMGKRIGEAVGEVEFSVRILDYYAKNAERFL
                     APVKLHPTNGEAHMESSPIGVIFCVEPWNFPYYQLARVAGPHLMAGNVVVVKHAGIVP
                     QCAIAFEKLWLEAGAPAGLYTNLLISHDQSNRIVDDPRIKGVALTGSVAAGQSIAARA
                     GRNLKISSMELGGSDAFIVLDDADLEHAVKWAVWGRMYNTGQTCCAAKRFIVVEKIAD
                     KFLEKFQAALQALKAGDPLDEKTTLGPLSTEAALQDLLKQVDGAVAKGAKVLMGGKRI
                     DRPGSYMEPTILTDIKPDNPAFREEFFGPVALFFRVKNEDEAIALANDSDFGLGGSVF
                     TKDIARGKRVASRIDTGMMFINNIDWSDAELPFGGIKNSGYGRELGDMGIQEFVNKKL
                     VRVSSAGAPV"
     gene            complement(5204..7270)
                     /locus_tag="VGC27_06675"
     CDS             complement(5204..7270)
                     /locus_tag="VGC27_06675"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY0282288.1"
                     /translation="MRRSVFALVGLVLAGCTLAATTPPSAPPPPKATLGTWGVDLSGM
                     DKSVKPGDDFFQYVNGEWFKTAEIPADRSGTGSFEKLQILSEKRMREIVDGLEAQPYD
                     QLSPDERKLRDLYDAFLDQKQIEERGLEPVKADLDTIAGLRTKADVARAMGSVKLGTQ
                     SIFNVRIDIDDKNPDAYSVNLSQSGLGLPDRDYYLRDDKALAATRDAYKKYLADMLTL
                     AGFKNAEARAAKIFAVETEIAKVSWARADRRDQDKVYNPMPYGDLKKLAPQFVWDAFF
                     VEPGIPTTGPKGARQVIVAEKSAFPLIAKVFAKTPVSVWRDYLTVHYLHTYAPYLPQK
                     FDDTDFAFFGTVLGGRTAQLDRPTRGVQLLDETLGEVLGKVYVAKYFPPEAKTKANAL
                     VANLLKAYEQDIQTLDWMSDATKQKALEKIGQFTPKIGYPDKWRDYSAYVVARDDLIG
                     NIQRSHEFDWNRDLKRLDDPTDKSEWFMTPPTVNAYYNRSWNEIVFPAAILQPPFFDP
                     NADDAVNYGGIGAVIGHEISHGYDDQGSKYTGKGVFETWWTPEDRANFEARTTMLVNQ
                     YEAFEPLEGMHLNGKNTLGENIADLAGVSIALKAYHISLDGKPAPVIDGFTGDQRFFF
                     GFGQIWRLKYRESDIRKQVLSDPHSYAPFRVIGPTRNVDAWYDVFDVQPDDKYYLPPD
                     QRVRLW"
     gene            complement(7408..7710)
                     /locus_tag="VGC27_06680"
     CDS             complement(7408..7710)
                     /locus_tag="VGC27_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282289.1"
                     /translation="MADGDNLIRHLLFTLYYTGAGGTFRTTAKDDAAGPVRRGDYDRT
                     LSKMSGGLGGAQFAAGTMPRRWCGGPIPALYRAKHTGRNRVVGENELPAPAETHAS"
     gene            complement(7740..9806)
                     /locus_tag="VGC27_06685"
     CDS             complement(7740..9806)
                     /locus_tag="VGC27_06685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY0282290.1"
                     /translation="MRRSVFALIGFFLAGCTLVATTPPSAPPPPKATLGTWGVDLSGM
                     DKSVKPGDNFFEYVNGGWLKTAEIPPDRSSIGSFQLLRIESEKRMRAIVEELQGKPAD
                     SLSPEERKLRDLYDAFLDRKQIDDRGLEPVKADLDYLMALKSSKDVARAMASVRMGTT
                     SIFDLYIGVDDKNSNAYSVNLVQGGLGLPDRDYYLKDDKNLAAVREAYKKYLADMLTL
                     AGFKNAEARVARIYALEEKIAKAHWPVEARREADKVYNPMTYSELKKLAPGFYWDAAF
                     KEAGIPTTSPTGERKVIVAEKSAFPALAKIFRTTPVSVWRDYLVVHYLHTNSAYLPQK
                     FDDLDFAFYGVALGGRPQQLDRPTRGVNLLDRTLGEALGKVYVAEYFPPAAKAKAQAL
                     VANLLKAYEQDIQTLDWMSDATRQKALEKIHAFTPKIGYPDKWRDYSALEISRDDLVG
                     NIQRHRVFEWNRNVKRLDDPVDKSEWGMTPSTVNAYYNPSFNEIVFPAAILQPPFFDP
                     NADDAVNYGGIGAVIGHEISHGYDDQGSKYTGQGILETWWTPEDRKNFDARTAALVAQ
                     YETYEPLPGEHINGKLTLGENIADLAGSTIALKAYHISLDGKPAPVLDGFTGDQRFFL
                     GFAQVWRSVYREKAKRNQVLTDPHSASPFRVIGSTRNVDGWYDAFGIKPGDKYYLAPD
                     QRVRLW"
BASE COUNT         1772 a         3053 c         3179 g         1919 t
ORIGIN      
        1 gatcgtgcgg ccccgcttct ccggcgcggc caaagtcgcg gcgaacggca cgatctgctg
       61 ggcgacgctg gaggcggcac ccaccaggag cgaagccgcg accagggtcg gcgccgtcgg
      121 tgcgaccgcc gcggcggcga gcgccagccc caggatcagg aactgggcga cgatgacacg
      181 gcgccgttcg agcaggtcgc cgagaggcac cagcaagaac aggccggctg caaagccgag
      241 ctgcgtggcc gtcgggatca ggctggccgt cgcggaaccc ggaaatgcgc gctcgatcag
      301 cgccagcatc ggctggttgt agtaaatgtt ggcgacggcg atgccgcagg cgacggccat
      361 cgcgaaggtg gggacggagg acggggcggc gcgcacgggc aattccgtat atcggcacgg
      421 ggccgcgcgt aaagatagca taatcacggc gatttgacgg cagcgccggg aaccgcaacc
      481 ctgtattctg tgacagccat gcgaccgaac ggcggcagcg acatcagaca agagttcttc
      541 ctcgcggggc ggaaagccgc cgaggagaac ggcgcgggcg tcatcggctc gctgctgctg
      601 catggccttg ccgcactcct ggtgttgacc gtcatcgtca agagcgcgcc gcagacggcg
      661 cagaagcttt tgccgttcgt cgcggtggat ttggtgcgcc tcggcatgga gaccgagtct
      721 ccgccggaag cgcgccaagc gctggtgccg cagcagagag cggccaggag gcaagaggcc
      781 gcaagtccca ccgatgaagc tgttggaccg aacaggacca agccgccgct cgatacgctg
      841 gaggccaagc tgcgcgggct ggcgaggctg cggcaaacgc aaaacaatcc gaatgtcgcg
      901 gtggggcagg gggtctccaa tgtcgatgca tcgaacggcg cgcccggcga ccgcgccacc
      961 tacagcgtga gggattacgt ccgcgcccaa gtgctgcgcc gctggagcct caacctcacc
     1021 aaactggcgg ggcacagttt tgcggtcacg atccgcgtga cgatgaagcg cgacggcacc
     1081 atcctctcgg ccgagatcgt cgagcggacg aacgatcctt tgtaccgcga cctcgcgctc
     1141 agcgcgcgca acgcggtgat cctcgcttcg cccatcgccc tgccgcccgg cgacctaccc
     1201 gccacgatgc gcttcacctt ggacctcgat cccaaagacg cgcggcactg atcacggcag
     1261 atcacgcgcc acgcggaagc cggcgaggct ggaataatcg tattccccgc cgctgcgcgc
     1321 gccgctgctg cgcgccgccg agcggacgaa gaccggcaga ttgttccacg aaccgccgcg
     1381 cagaacatgc ttggcgcagc ctttttccgt ccacgggcgg ccgtcgttcg gcgcgccgac
     1441 ataggagcgg tgccagcagt cggcggtcca ttgccagacg ttccccagca tcccgtaaag
     1501 cccgaacgga ttgggcgcga agctgttcac ctcggccagc acgcgattgt cccatttgct
     1561 gccgcagccg ttgcagttgg cgttgccgga gccgatctcg gcgccccacc agcgcgcggt
     1621 ggtggtgccg gcgcgcgccg catattccca ttccgcttcg ctcggcaggc ggtaagggtt
     1681 cttcgttatt gccggatgtt cgagccgcac cttcttgttc agccaggcga tgtaggcttc
     1741 ggcgtctttc caatccagac acgacgccgg ccacaggcgc ggttcctcgt cgtaaggcgg
     1801 ataggcgtag ccgccgccga gcgaacgcca gcgcatgtcg agcagcgcgt tgcagggttc
     1861 gggttgatag ccggtctctt tgaggaagag caggaactcc tcgctggtca cgttatattt
     1921 gccgagcgcg aacgccttga tcgaaaccac atgctgcggg ccttcatagt cgaagcggcc
     1981 gggttcgctt gccgggctgc ccatgacgaa cttgcccgcg gggatgccga ccatcagcgg
     2041 acagccggcg cattccacga actcgcgctc ctggcgctcc tgcgcggacg cgctcgccgc
     2101 cgaggcgaac gcggcggcga agagcaggac ggacgaaaat ctgtgacgcc aattcatgcg
     2161 cagctcgctt ggtgacggaa tcagcttaca ggtttcggcg ggcgcgcaac gcagcaggct
     2221 tgccgcgccc ggtcaccgct gcgtgagggc cgtctgccgg acggcggggc cgtctgccgg
     2281 atgcgcggtt tacctaaccc actgaaataa ccgcataaac gccggaaggt tgttttcgac
     2341 ttttcaaagg gggtgggggg tgcccgccct ttcccatctc tgcggccgcc gccggtggtg
     2401 aagcgatttg cttggtggtg aagcgatttg ctttttgcgt ctccttttga ctgcgcgacc
     2461 gaagcgatat gggaattcag tcagcgcggt tcaagagcgc gcgaaggatt tttttgcgcg
     2521 agaaagtctc gcaatcatcg cggccgttag aactggataa actcgaagta taaaacacaa
     2581 ttgaagaagc tgcttacgcg acaaagccgc gaccgaaaaa aagaagcgcc gacaattgaa
     2641 gaacgcgcta tttttggccg ggaccaacag aaagcgagca acacgatgtt aacacgacgc
     2701 caaaccaatg ccggaattct cgccagcgcg gcgtttgccg tcacatcggc cgtggcggtc
     2761 ggccaggagt ccaagcttcg cactctgccg cccccgcgga gcaaaggggg aatgccgctg
     2821 acggaagcgc tcttgctgag gcgttccacg cgcgactatt cggagcggcc gattcccccg
     2881 caggttctgt ccgatctgtt gtggtcggcg ttcggcgtca atcgtccaaa cggcgagcgc
     2941 accgctccct attggcgcca tatcatggtg acggacattt atctggcgat ggcggacggc
     3001 gtatggctct acgaaccaaa agcgcacgcg ctgctgccgc attcaggtga agatatccgc
     3061 gcgctgaccg gggcccagaa atttgcgacc accgctccgc tcaacctcgt ctatgtcgcg
     3121 catggcgagc gcatgacgga tgtttctctc gaagaccgcc gcctgtttgc ctcggtcaac
     3181 accggcttca tcgggcagaa cgtctatctg ttctgcgctt ccgaggggct ggcgacggtt
     3241 tttcgcggat cggtcgatta tccgaaactt gcccaggcgc tgaagcttcc cgagcagcag
     3301 ttcgtgacct ttgcgcagac ggtcggttat cccaaaggct gagcgccggt ccggcggacg
     3361 agcatttttg cgccggcgct aaaacagcgc cgccggagcg atccactcgc tcgaaccgct
     3421 ggaatcgcaa gcttcagaca ggcgcgccgg cggacgaaac gcgaaccagc ttcttgttga
     3481 cgaattcctg gatgcccatg tcgccaagtt cgcgcccata acccgaattc ttgataccgc
     3541 cgaacggcaa ttcggcgtcc gaccaatcta tgttgttgat gaacatcatc cccgtatcga
     3601 tgcggctggc cacgcgcttt ccgcgcgcga tgtccttggt gaagacggag ccgcccaagc
     3661 cgaagtcgga gtcgttggcg agcgcaatgg cctcgtcctc gttcttgacg cggaagaaca
     3721 gggcaactgg gccgaagaac tcctctctga acgccgggtt gtccggcttg atgtcggtca
     3781 ggatcgtggg ctccatgtac gagcccgggc gatcgatgcg cttgccgccc atgagcacct
     3841 tggcgccctt ggctacggcg ccgtcgacct gtttcagaag gtcctgcaac gccgcttccg
     3901 tggagagcgg ccccagggtc gtcttctcgt ccagagggtc gccggccttg agcgcctgga
     3961 gggcggcttg gaacttttcg aggaacttgt cggcgatttt ctcgacgacg atgaaccgct
     4021 tcgcggcgca gcaggtttgt ccggtgttgt acatcctgcc ccagacagcc catttgacgg
     4081 catgctcgag gtccgcatcg tcgagaacga tgaacgcatc gctgcctccg agttccatcg
     4141 aggaaatctt gaggttgcgt cccgctcttg ccgcgatgct ctggcccgcc gcgacgctgc
     4201 ccgtcagcgc gacgcccttg atccggggat cgtcgacgat gcggttcgac tgatcgtgcg
     4261 aaatcagcag attggtgtaa agcccggcgg gcgcgcccgc ttcgagccaa agcttctcga
     4321 aggcgattgc gcattgtggc acgatgcccg cgtgtttcac caccaccacg ttgcccgcca
     4381 tcaagtgggg gccggcgacg cgcgccaact ggtaataggg gaagttccac ggctcgacgc
     4441 agaagatcac gccgatcggg ctgctctcca tatgagcttc tccgttcgtc ggatggagct
     4501 tcaccggcgc caggaagcgc tcggcgttct tggcgtagta gtcgagaatt ctcacgctga
     4561 actccacctc gccgacggcc tcgccgatgc gctttcccat ctcgagagtc atcgtccgtg
     4621 cgaactcggc ggccttcgcg tgcaagagtt cagccgcttt ggccacgatg accgcccgtt
     4681 cggcataggt cttgtgccgc cagatttcgt aacaagccgc ggcggccgca agcttggtct
     4741 cgagttcctt gtccgtcagc tcctcgaatt tcttcaagag cttgccggtc gccgggttga
     4801 agctttgata ggccatgggt tcggtccctt tgcgctatga caatgcggga aaacggctgg
     4861 cgccgccgtg gttgcactcg agcctggatc gctgtttcga gggcggatgc ggccaatggc
     4921 attgcgcaaa gccttcgcca gcgagcttga ccgatgccgg ccacgcttgg ttgacggccg
     4981 ggaccataac cgccggtcaa gcgcgaacgg taggctcgga aactactcag ccaagagcga
     5041 acgcccgcta accttggtgc tattgcgcca agccggtcgt gtctgcggtg agacggtgcc
     5101 tgagccttcg ccggcacgcg atcattctat gacgggtaag caagatggcg aatgaggttg
     5161 cccccatccg ccatttgccg gtcctacctt gcaggctacc tcgtcaccac aaccgcacgc
     5221 gctggtcggg cggcagataa tatttgtcgt ccggctggac gtcgaagacg tcgtaccagg
     5281 cgtcgacatt gcgggtcgga ccgatgacgc ggaacggcgc gtaggaatgc gggtcagaca
     5341 agacctgctt gcgaatgtcg ctctcgcggt atttcaaacg ccagatctgc ccgaaaccga
     5401 agaagaaacg ctgatcgccg gtgaagccgt cgatgaccgg cgccggcttg ccgtccagcg
     5461 agatatggta ggccttcagc gcgatggaga cgcccgccag gtcggcgatg ttctcgccca
     5521 gcgtgttctt gccgttgagg tgcatgccct ccagcggctc gaaggcttcg tactggttga
     5581 ccagcatggt ggtgcgcgct tcgaagttcg cgcgatcctc gggcgtccac caggtttcga
     5641 acacgccctt gccggtgtat ttcgagccct ggtcgtcgta gccgtggctg atctcgtggc
     5701 cgatcaccgc gccgatgccg ccgtaattca ccgcatcatc ggcgttggga tcgaagaagg
     5761 gcggctggag gatggcggcc gggaacacga tctcgttcca cgaccgattg taataggcgt
     5821 tgaccgtggg cggcgtcatg aaccattcgc ttttgtcggt gggatcgtcg agccgtttca
     5881 ggtcgcggtt ccagtcgaac tcgtggctgc gctggatgtt tccgatcaag tcgtcgcgcg
     5941 ccacgacata ggccgaatag tcgcgccact tgtcgggata gccgatcttg ggcgtgaatt
     6001 ggccgatctt ctccagcgcc ttttgcttgg tggcgtcgct catccagtcg agcgtctgga
     6061 tgtcctgttc gtaggctttc agcagattgg cgaccagcgc attggccttg gtcttggctt
     6121 ccggcgggaa gtatttggcg acatagacct tgcccagcac ttcgcccagc gtttcgtcga
     6181 gcagctgcac gccgcgcgtc ggccggtcga gttgcgccgt gcgcccgccg agcaccgtgc
     6241 cgaaaaaggc aaagtcggtg tcgtcgaact tctgcggcag atagggcgcg taagtatgca
     6301 ggtaatggac ggtgagatag tcgcgccaca ccgagaccgg cgtcttggcg aagaccttgg
     6361 cgatcagcgg aaacgccgac ttctcggcga cgatcacctg gcgggcgccc ttcggtccgg
     6421 tcgtcggaat gccgggttcg acgaagaagg cgtcccagac gaactgcggc gcaagcttct
     6481 tgaggtcgcc atagggcatc ggattgtaaa ctttatcctg gtcgcggcgg tcggcgcggg
     6541 cccacgacac cttggcgatc tcggtttcca ccgcgaagat cttcgcggcg cgcgcttcgg
     6601 cgttcttgaa gccggccagg gtcagcatgt cggcgaggta tttcttgtag gcgtcgcgcg
     6661 tggcggccag cgccttgtcg tcgcgcagat agtaatcgcg gtcgggcagg ccgagaccgg
     6721 attgcgatag attgacggaa taggcgtcgg gattcttgtc gtcgatgtcg atcctgacat
     6781 tgaagatgct ttgcgtgccg agcttgaccg agcccatggc gcgcgccacg tcggccttgg
     6841 tcctgagacc ggcgatagtg tcgaggtcgg ctttgaccgg ctcaaggccg cgctcctcga
     6901 tctgcttctg gtcgaggaag gcgtcgtaaa ggtcgcgcag cttgcgttca tcgggcgaga
     6961 gctggtcgta aggttgcgcc tccagcccgt ccacgatttc gcgcatgcgc ttctcgctca
     7021 aaatctggag cttctcgaat gagccggtgc ctgaacggtc cgccgggatt tccgcggtct
     7081 tgaaccattc gccgttgacg tattggaaaa agtcgtcgcc gggcttgacg ctcttgtcca
     7141 ttccgctgag atcgacgccc caagtaccga gcgtcgcctt cggcgggggc ggcgcggacg
     7201 gtggggtggt ggcggccaac gtgcagccgg ccaaaacaag accgacaagc gcgaaaacac
     7261 ttcttcgcat ggcacactcc gtaataaggt gaaagcgcgg accagcccgt ccggccaaag
     7321 gcgattttgg gaaatctgtc aggagaatgg ctaccggcgc aagtcccggt cccgagaggt
     7381 cagctaaatt gccactgaca acatccgtca ggaggcgtgg gtttccgctg gggcagggag
     7441 ttcattctcg ccgaccacgc ggttccggcc cgtgtgcttg gcgcggtaga gggcaggtat
     7501 cggccctccg caccagcgtc tcggcatggt cccggcggcg aattgagcgc cgcccaagcc
     7561 gccgctcatc ttcgacaggg tgcggtcgta atcgcctcgt cgaactggtc ctgctgcatc
     7621 gtctttcgcc gtcgtcctga aggtaccgcc ggcacccgta tagtaaagcg tgaaaagcaa
     7681 atggcgaatg aggttgtccc catccgccat ttgccggtcc taccttgcag actacctcgt
     7741 caccacagcc gtacgcgctg gtccggcgcg aggtaatatt tgtcgcccgg cttgatgccg
     7801 aaggcgtcgt accagccgtc cacgttgcgg gtcgatccga tgacgcggaa cggcgaggcg
     7861 gaatgcgggt cggtcagaac ctgattgcgc ttggcctttt cgcgatagac cgaacgccag
     7921 acctgcgcga agccgaggaa gaagcgctgg tcgccggtaa atccgtcaag caccggcgcc
     7981 ggcttgccgt ccagcgagat atgataggcc ttcagcgcga tcgtcgagcc ggcgaggtcg
     8041 gcgatgttct cgccgagcgt cagcttgccg ttaatgtgct cacccggcag cggttcgtag
     8101 gtctcgtatt gcgccaccag cgccgcggtg cgcgcatcga agttcttgcg gtcttccggc
     8161 gtccaccagg tttcgagaat gccctggccg gtgtatttcg acccctggtc gtcgtagccg
     8221 tggctgattt cgtgcccgat caccgcgccg atgccgccgt aattcaccgc gtcgtcggca
     8281 ttaggatcga agaagggcgg ctgcagaatg gcggccggga acacgatctc gttgaacgac
     8341 ggattgtagt aggcgttgac cgtggacggc gtcatgcccc attcgctttt gtcgacggga
     8401 tcgtcgagcc gcttcacgtt gcggttccat tcgaagacgc gatggcgctg gatgttgccg
     8461 accaggtcgt cgcgcgagat ttcaagggcg gaataatcgc gccacttgtc gggatagccg
     8521 atcttgggcg tgaaggcgtg gatcttttcc agcgctttct gcctggttgc atcgctcatc
     8581 cagtcgagcg tctgaatatc ctgttcatag gctttcagca gattggcgac gagcgcctgg
     8641 gccttggcct tggccgccgg tgggaaatat tccgcgacat agaccttgcc cagcgcttcg
     8701 cccagcgtgc ggtcgagcag attcacgccg cgcgtcggac ggtcgagctg ctgcggccgc
     8761 ccgccgagcg ccacgccgta gaaggcaaag tcgagatcgt cgaatttctg cggcagatag
     8821 gcggaattcg tgtgcagata gtggaccaca agataatcgc gccataccga aaccggcgtc
     8881 gtcctgaaga ttttcgccag agccgggaac gccgactttt cggcgacgat caccttgcgc
     8941 tcgcccgtgg ggctcgtcgt gggaatgccg gcctccttga acgccgcatc ccaatagaat
     9001 cccggcgcca gcttcttgag ctcgctatag gtcatcgggt tatagacttt gtccgcttcg
     9061 cggcgggctt cgaccggcca atgcgccttg gcgatcttct cttccagcgc gtagatgcgg
     9121 gcgacgcgcg cctcggcgtt cttgaagccg gccagcgtca gcatgtcggc gaggtatttt
     9181 ttataggcct cgcgcacggc cgccagattc ttgtcgtctt tcagatagta gtcgcggtcc
     9241 gggaggccca acccgccctg cacaaggttc accgaatagg cattcgaatt cttgtcgtcc
     9301 acgccgatat agagatcgaa gatgctggtg gttcccatcc tgaccgaagc catggcgcgc
     9361 gccacatcct tgctcgactt cagcgccatg agatagtcga gatcggcctt caccggctcg
     9421 agaccgcgat cgtcgatctg cttccgatcg agaaaggcgt cgtagagatc gcgcagcttg
     9481 cgctcttcgg gcgacagcga atccgccggt ttcccctgaa gttcctcgac gatcgcgcgc
     9541 atccgcttct cgctctcgat gcgaaggagc tggaaggaac cgatgctgct gcggtcgggc
     9601 gggatttccg cggtcttgag ccagccgccg ttgacatatt cgaagaaatt gtcgccgggc
     9661 ttgacgctct tgtccattcc gctgagatcg acgccccagg taccgagcgt cgccttcggc
     9721 gggggcggcg cggacggcgg ggtggtggcg accaacgtgc agccggccag gaaaaaaccg
     9781 ataagcgcga aaacactcct tcgcatggcg tactccgtaa taaggtgagg gcgcggacca
     9841 gcccgtccgg ccgaagacga tttcggggaa tctgtcagca gaatgcctgc cggcgcaagt
     9901 cacggtcccg cgagatcagc caa
//
LOCUS       DASWPU010000312         5746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_33550,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000312 DASWPU010000000
VERSION     DASWPU010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33550"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /gene="ppk1"
                     /locus_tag="VGC27_06690"
     CDS             complement(<1..1385)
                     /gene="ppk1"
                     /locus_tag="VGC27_06690"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY0282291.1"
                     /translation="MPKAKTKVEVVEGAPRSDALAPAVEDVSAPEEGESFAPQESIVA
                     PAIDPGSPERFVNRELSWLAFNGRVLEEAQNLRHPVLERLRFLSISASNLDEFYMVRV
                     AGLVGMVNEGVVTTSADGLSPSEQLVHIGQMAGKLMSDQQRVWISLQAELREAGVCVI
                     EPAEMNAADKEWLDGYFRSQIFPVLTPLAIDPAHPFPFIPNLGFSLALKLLRKRDGKA
                     MMALLPVPAQLRRFIRLPGEGKEARFLALEKVIGLYMDRLFPGHAVTGMGQFRILRDS
                     DVEVEEEAEDLVLMFESLLKRRRRGSVIHMMCSQSMPDDLRRFIADHLQLKLEEFIFV
                     EHMLGLSDLTKLILKERADLQFKPYIARFPERIRDHGGDCFASIRAKDIIVHHPFESF
                     DVVVQFLRQAAADPEVVAIKQTLYRTSDDSPIVSALIEAADSGKSVTALVELKARFDE
                     AANIKWARDLER"
     gene            complement(1451..2107)
                     /locus_tag="VGC27_06695"
     CDS             complement(1451..2107)
                     /locus_tag="VGC27_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282292.1"
                     /translation="MPSQLPLPLETRRDFTREDFIVAAGNSAAVGFVDSFPAWPSPAA
                     ALYGPAGSGKSHLAAVWAAKSGARIVEAEALDEAVLAQGSAALAVENVDHAPLSAAAE
                     TALFALIERGRPLLLTGREAPSAWPARLPDLVSRFRALLGFGLWAPDDALLEALARKL
                     FADRQLSVPDTVVTQIIRALERSPAAMRDFVARADAAALAQKRPITPGLIRELLSKDA
                     "
     gene            complement(2113..2715)
                     /locus_tag="VGC27_06700"
     CDS             complement(2113..2715)
                     /locus_tag="VGC27_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0282293.1"
                     /translation="MATLARRRRNYGRAVNALLRQTPNILSALRLIAAPIAAVLILDG
                     VDFVAVCVFVAAGLSDALDGYLARRFALTSRFGAWLDPVADKLLMLASFVTLTIVGAV
                     PLWLTIVVIGRDVAIVLGVGLARLLDAPLRIAPLMIGKASTVVQVVFVALVLLGLSVG
                     LSAPRLEFAGCVVVALLTLSSFFAYAYVWLQALAAGRRAA"
     gene            complement(2670..3743)
                     /locus_tag="VGC27_06705"
     CDS             complement(2670..3743)
                     /locus_tag="VGC27_06705"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEY0282294.1"
                     /translation="MRHILFFPGLALALAVFCFSPHPAAADSPFTVADVSVDASSASA
                     AEARNEAVAAGRPTAWQILFRRLTRQQDWVRQPVLDDTQLQRLITTYYPSNERRSTRR
                     YTADMTYVFNQEAVSRLLQSSGIPYAASAAKRVLVIPMAPAYSRGSPWTAALSNPRFA
                     TGVVPISVPAGDASDMSVLGGLTFDTAKWTDVEPVAARIRASEAVLVQAVAAGNKLTV
                     TLRRLGAGELPTKTSFEVPLLQGAASTYPTAADAAVHAIEDMWKSHAAVDFSQKGKLV
                     VDVRVGSLPQFAGMQNALAGVPNVAGVTVVAMSIGDARLSLSYLGTVDQLREALAQAG
                     LLLVKSGGAWQLSQGAGAITGAP"
     gene            3950..5029
                     /gene="purM"
                     /locus_tag="VGC27_06710"
     CDS             3950..5029
                     /gene="purM"
                     /locus_tag="VGC27_06710"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY0282295.1"
                     /translation="MTTNQNSGPGGLTYKDAGVDIDAGEALVKRIGPAAKATRRPGAE
                     ADLGGFGGLFDLKAAGFTDPVLVAATDGVGTKLKIAIDTGLFAGIGQDLVAMCVNDIV
                     VQGAEPLFFLDYYASAKLNVNAAAAVIEGIARACKESGCALIGGETAELPGLYAKGDF
                     DLAGFSVGAAERGAILPKTAEMLAGDVLIGVASSGVHSNGFSLVRRVVEQSGLSYDAP
                     APFAPGIALGEALLTPTRLYVKSALAAIRTGGIKGLAHITGGGLTENTPRALPAGLDA
                     EIDLGLWTPPSVFGWLAKSAGIEEREMLRTFNCGIGLIVVASEKCSGRVIDAFQENGD
                     KAVRLGRLAPGKGEAKAHYRGALKL"
     gene            5026..5631
                     /gene="purN"
                     /locus_tag="VGC27_06715"
     CDS             5026..5631
                     /gene="purN"
                     /locus_tag="VGC27_06715"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY0282296.1"
                     /translation="MKARTAILISGRGSNMRSLIAAAADPAFPAEIVLVISNVGNAEG
                     LKHAANAGIATKVIPHKAFESREAFDAEIDAALLAAGTDIVCLAGFMRILSDWFARRW
                     EGRLLNIHPSLLPSFKGVHVHEQVLQAGARVSGCTVHFVVPELDAGPIISQAAVPVEL
                     GDTPDTLAARVLQVEHKLYPEALKLLAEGKVRLEAGRAVFA"
BASE COUNT         1055 a         1893 c         1885 g          913 t
ORIGIN      
        1 cgttccaggt cgcgcgccca tttgatgttg gcggcctcgt cgaagcgcgc cttcagctcg
       61 accagcgcgg tgacggactt gcccgaatcg gcggcctcga tcagtgcgct gacgatcggc
      121 gaatcgtcgc tggtgcggta cagcgtctgc ttgatcgcca ccacctcggg atcggccgcc
      181 gcctggcgca ggaactgcac caccacgtcg aagctctcga aaggatggtg aacgatgatg
      241 tccttggcgc ggatcgacgc gaagcagtcc ccgccgtgat cgcggatgcg ttccggaaaa
      301 cgcgcgatgt aaggcttgaa ctgaaggtcg gcgcgctcct tgaggatcag cttggtcagg
      361 tccgacaagc cgagcatgtg ctcgacgaag atgaattcct ccagcttcaa ctgcaggtga
      421 tcggcgatga accggcgcag atcgtccggc atcgactggc tgcacatcat gtggatgacc
      481 gagccgcgcc gccgccgctt gagcagcgat tcgaacatca gcaccaggtc ctcggcctct
      541 tcctccactt ccacgtcgct gtcgcgcagg atgcggaact gtcccatgcc ggtgaccgcg
      601 tggccgggga acagccggtc catgtagagc ccgattacct tctcgagcgc caggaagcga
      661 gcctccttgc cttcgccggg caagcggatg aagcgccgaa gctgcgcggg caccggcagc
      721 agcgccatca ttgccttgcc gtcgcgctta cgcagcagct tgagcgccag cgagaagccg
      781 agattgggaa tgaagggaaa gggatgcgcg ggatcgatgg cgagcggcgt cagcaccgga
      841 aaaatctggc tgcggaagta cccgtccagc cattccttgt cggcggcgtt catctcggcg
      901 ggttcgatga cgcagacgcc ggcctcacgc aattccgcct gaagcgatat ccagacgcgc
      961 tgctggtcgc tcatgagctt gccggccatc tggccgatat ggaccagttg ctcgctgggg
     1021 ctgaggccgt cggcgctggt cgtgacgacg ccttcgttga ccatgccgac aaggccggcc
     1081 acgcgcacca tgtagaattc gtccaggttc gaagcggaaa tcgacagaaa ccgcagccgc
     1141 tccagcaccg gatggcggag gttctgagct tcctccagca cccgcccgtt gaaggccagc
     1201 caggacagtt cgcggttgac gaagcgttcc ggcgagccgg gatctatggc aggcgcgacg
     1261 atgctttcct ggggcgcaaa gctctcccct tcttcgggag cggaaacgtc ctccacggcc
     1321 ggcgccaggg cgtcgcttcg cggcgcgccc tcaaccacct caactttggt ctttgctttt
     1381 ggcatcaatg ggttgtcgca aatgagcggc tacagcgtaa agcaaaaatg cggcactgtc
     1441 atgccgcagc ttacgcgtct ttcgacaaca attccctgat aagtccaggc gttattgggc
     1501 gtttttgggc caaagccgcg gcatcggccc gggccacgaa atcgcgcata gcggcgggcg
     1561 agcgctccaa agcgcgaatt atctgcgtca ccaccgtgtc cggcacgctc aactggcgat
     1621 cggcgaagag ctttctcgcc aaggcttcca gcagcgcgtc gtccggcgcc cacagcccga
     1681 accccagcag agcgcggaac cgcgatacca gatccggcag gcgcgcgggc caagcggagg
     1741 gcgcctcacg gcccgtcagc agcaggggcc ggccgcgctc gatcagggca aaaagcgccg
     1801 tctcggccgc ggcgctcaac ggcgcatgat cgacattctc caccgccagg gccgcggaac
     1861 cttgcgccag caccgcctcg tcgagcgcct cggcttcgac gatccgcgcg ccgctcttcg
     1921 ccgcccaaac ggccgccaga tgcgacttgc ccgaaccggc cggtccgtaa agcgctgcgg
     1981 caggcgaagg ccaagccgga aaagagtcga caaatccgac ggccgcgctg ttcccggcag
     2041 cgacgatgaa atcctcgcgc gtgaaatcgc ggcgcgtttc gagcggcaaa ggaagctgtg
     2101 agggcatccc ggtcaggcgg cccgccggcc ggcggcaagc gcttgcagcc acacataggc
     2161 gtaggcgaaa aacgacgaca gggtgaggag cgccaccacc acgcatccgg cgaattccaa
     2221 ccgcggcgcg ctcagaccga ccgacagtcc caacaggaca agcgcgacaa agacgacttg
     2281 caccacggta ctcgccttgc cgatcatcaa gggcgcgatc cgtaacggcg cgtccagcag
     2341 ccgcgccagg ccgacgccca gcacgatcgc cacatctctc ccgatgacca ctattgtcag
     2401 ccacagcggc accgcgccca cgatcgtcag ggtgacgaag ctcgccagca tcagaagttt
     2461 gtcggcgacc ggatcgagcc aggcgccgaa acgggaggtt aaagcaaagc gcctggcgag
     2521 atacccgtcc agcgcgtcgc tgaggccggc ggcgacgaaa acgcaaaccg ccacgaaatc
     2581 gacgccgtcg aggatcagaa ccgcggcaat aggagcggct atgagcctga gcgcgctcag
     2641 gatgttcgga gtttggcgca gcagcgcgtt cacggcgcgc ccgtaattgc gccggcgcct
     2701 tgcgagagtt gccacgctcc cccgctcttg acgagcagga gacccgcctg cgccagcgcc
     2761 tcccgcaact gatcgacggt gccgagatag gaaagcgaca gacgcgcatc gccgatgctc
     2821 atggcaacga cggtgacacc cgccacattg ggaacgcccg cgagcgcatt ctgcatgccg
     2881 gcaaactggg gcagcgaacc cacgcgcaca tccacgacca gcttgccctt ctggctgaaa
     2941 tcgaccgcgg catgggattt ccacatgtcc tcgatggcat gcacggcggc atccgcggcg
     3001 gttggatagg tggacgccgc gccctgcagc aagggcactt cgaagctggt cttggtcggc
     3061 aattcgcccg cccccaggcg gcgcagcgtg accgtgagct tattgccggc cgccacagcc
     3121 tgaaccagca ccgcttcgct ggcgcgaatc cgcgcggcga cgggctctac gtccgtccat
     3181 ttcgcggtat cgaaggtaag cccgcccaag acactcatgt ccgacgcatc gccggccgga
     3241 accgagatcg gaaccacacc ggtggcgaag cgcgggttgg acaaggcggc ggtccacggc
     3301 gagccgcgcg aataggcggg cgccatcgga atcaccagca cgcgctttgc cgccgatgcc
     3361 gcatagggga tgccggaact ctgcaacagg cgggacacgg cctcttgatt gaacacatag
     3421 gtcatgtccg ccgtgtagcg cctggtcgac cgcctctcgt tcgacgggta ataggttgtg
     3481 atcagccgct ggagctgggt gtcgtcgagc acgggctggc ggacccagtc ctgctggcgg
     3541 gtgaggcggc ggaacagaat ctgccacgcc gtcggcctgc cggccgcgac cgcttcgttc
     3601 cgggcctcgg cggccgatgc cgatgacgcg tcgacagaaa cgtccgcgac cgtgaacgga
     3661 ctgtctgcgg cagccgggtg cggcgaaaaa caaaagacag caagcgcaag agcaagcccc
     3721 ggaaagaaaa gaatgtgtcg catggagatc ctgttccgaa cgcggcgact atagtcggcg
     3781 ttcgcggccc ttgaaagcgg cggattggct tttccgaagc ggcccggcgg cgacgggatc
     3841 gcgccgcaac ccgcctgctc cgcgggtccc ggcttcgcaa cagtatcgct tcttgcgcgg
     3901 ccgctttagc gctattagac ggagctgtga cccaacttca ccgcgcacca tgacgacgaa
     3961 tcagaattcc ggccccggag gcctcaccta caaagacgcc ggcgtcgata tcgacgccgg
     4021 cgaagcgctg gtcaagcgca tcggccctgc cgccaaggcg acgcggcggc ccggtgcgga
     4081 ggccgatctg ggcgggttcg gcggcttgtt cgatctcaag gcggcgggct tcaccgaccc
     4141 ggttctggtc gccgccaccg acggcgtcgg caccaagctc aagatcgcca tcgacaccgg
     4201 gctcttcgcc ggcatcggcc aggacctggt ggcgatgtgc gtcaacgaca tcgtggtgca
     4261 gggtgccgag ccgctgttct tcctcgacta ttatgccagc gccaagctga acgtgaacgc
     4321 tgcggcggcg gtgatcgagg gcatcgcccg cgcctgcaag gaaagcggct gcgcgctgat
     4381 cggcggcgag accgcggaat tgccgggtct ttacgccaaa ggcgatttcg atctggcggg
     4441 attttccgtc ggcgcggcgg agcgcggcgc gatcctgccc aagacggcgg agatgctggc
     4501 gggcgacgtg ctgatcggtg tcgcctcttc cggtgtgcat tccaacggct tctcgctggt
     4561 gcgccgcgtg gtggagcaga gcggactgtc ctatgacgcg ccggcaccct tcgcgcccgg
     4621 cattgctctc ggcgaggcgc tgctgacgcc gacgcggctc tatgtgaaaa gcgcgctcgc
     4681 cgcgatccgc accggaggca tcaaagggct ggcgcacatc accggcggcg gcctcaccga
     4741 gaacacgccg cgcgccctgc ccgccggact cgacgccgag atcgatctcg gcttgtggac
     4801 gccgccctcc gtcttcggct ggctggcgaa atcggccggc atcgaagaac gcgaaatgct
     4861 gcgcaccttc aactgcggga tcggactgat cgtcgtggcg agcgaaaaat gctccggccg
     4921 ggtgatcgac gccttccagg agaacggcga caaggcggtg cggctgggca ggctggcgcc
     4981 gggcaagggc gaagcgaagg cgcactatcg cggagcattg aagctgtgaa agcgcgcacc
     5041 gccattctga tctccgggcg cggcagcaac atgcgttcgc tgatcgcggc ggccgccgat
     5101 ccggctttcc cggccgaaat cgtcctggtg atttccaatg tcgggaacgc ggaaggcctg
     5161 aagcacgcgg cgaacgcggg catcgccacg aaagtcatcc cccataaagc ctttgaatcg
     5221 cgcgaagcgt tcgatgcgga aatcgacgcg gcgctgctcg cggccggcac cgacatcgtc
     5281 tgcctggccg gcttcatgcg catcctgtcc gactggttcg cgcgccggtg ggaagggcgg
     5341 ctgctcaaca tccatccttc gctgctgccg tcgttcaagg gcgtgcatgt gcatgagcag
     5401 gtgttgcagg cgggcgcgcg ggtttccggc tgcaccgtgc atttcgtggt gccggaactc
     5461 gacgcaggcc ccatcatctc ccaggccgcg gtgccggtgg agcttggcga cacgcccgac
     5521 actttggccg cgcgcgtgct gcaagtcgag cacaagcttt atcccgaagc gctgaagctg
     5581 ctggcggaag gaaaagtcag gcttgaagcc gggcgggcgg tattcgctta agcccccact
     5641 cctgcgatct gcgccgagac ttcccacagc cgcttcgcgt cggcgtcgtt ttgcgcggcg
     5701 gctgtcggcg tggcgggggc gcttttgtag taatagccgc cgctct
//
LOCUS       DASWPU010000313         1919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_21325,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000313 DASWPU010000000
VERSION     DASWPU010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21325"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VGC27_06720"
     CDS             <1..1116
                     /locus_tag="VGC27_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0282297.1"
                     /translation="PEAPGFAYADSRLIADVYGPRETAAAAERYSRKIHKIDGVICVA
                     TDAPLTTAEVCERLGIAGLSRASAELACDKLAMKRRFAEAGVPIPWFSEVETLQALQR
                     IAIERGRDLVIKPADSRGSRGVQRISHVEDLAKAFLFARSHSPSERVMVEQYLDGPQI
                     STESIVVDGRCYTPGFSDRNYEYLERYAPFFIENGGDLPSHLAPELQAKVKDVVGRAA
                     AALGVTNGTVKGDIVVHDGEVYVIELAARLSGGFFCTREIPLNTGVDFVGNAIKVALG
                     DPVDPADLEPKYQKPVIQRYAFPPAGRVVSISGAEEARKVPGIAEVVVTAKLGDVIPP
                     AGDKRPSAAMVLASGATRDIALEAANDALARIRIRTA"
     gene            1113..>1919
                     /locus_tag="VGC27_06725"
     CDS             1113..>1919
                     /locus_tag="VGC27_06725"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282298.1"
                     /translation="MNAPFPSRQFDLATGTLRLFAFFHLNLAFSSIEEERRGEVIAQC
                     YWPLLRLAERCGPVGVEASGYTLEEIAKLDPAWIEALKRLIGAGQVDFIGAGYSQMIG
                     PLVPYRVTQANLAIGNETYRRLLGIRPSIALVNEQAYSGGLVGLYLDAGYRTLLMDWD
                     NPSSHHQWPAEARYAPQRAAGADGRTIGLIWTNTVAFQKLQRFAHDDIGLDDYLAYVR
                     AQRGTVARALCLYASDAEVFDFRPGRFRTEEKNNGGEWERLEQAFERLAQE"
BASE COUNT          332 a          642 c          609 g          336 t
ORIGIN      
        1 cccgaagcgc ccggctttgc ttatgcggat tcgcggctga tcgcggacgt ctacggcccg
       61 cgcgaaacgg cggcggcggc ggaacgctat agccgcaaga tccacaagat cgacggcgtc
      121 atctgcgtgg cgacggacgc gcctttgacc acggccgaag tctgcgaacg tctcggcatc
      181 gcgggccttt cgcgcgccag cgccgaactg gcgtgcgaca aactggcgat gaagcggcgc
      241 tttgccgaag cgggcgttcc catcccctgg ttttccgaag tcgaaaccct gcaagccttg
      301 cagcgcatcg ccatcgagcg cgggcgcgac ttggtgatca agcctgccga cagccgcggc
      361 tcgcgcgggg tgcagcgcat ctctcatgtc gaagacttgg ccaaagcctt cctgttcgcc
      421 cgttcccatt cgccgagcga gcgcgtcatg gtcgagcagt atctggacgg cccgcaaatc
      481 tccactgaat ccatcgtcgt ggacggacgc tgctatacgc ccggcttttc cgaccgcaat
      541 tacgaatatc tggagcgcta cgcgccgttc ttcatcgaaa atggcggcga tctgccgagc
      601 catctggcgc ccgagctgca agccaaggtg aaggacgtgg tcgggcgcgc cgcggccgcg
      661 ctcggcgtga ctaacgggac ggtcaagggc gatatcgtcg tccatgacgg cgaggtctat
      721 gtcatcgaac tggcggcgcg gctgtccggc ggctttttct gcacccgcga gattccgctc
      781 aacaccgggg tcgatttcgt cggcaatgcg atcaaggtgg cgctgggcga tccggtggac
      841 cccgccgacc tcgaaccgaa ataccaaaag ccggtgatcc agcgttacgc cttcccgccg
      901 gcgggccgcg tcgtctcgat ctcgggcgcc gaggaagcac gcaaagtccc cggcattgcc
      961 gaggtggtcg tcaccgccaa gctgggcgat gtcattccgc cggccggcga caagcgcccc
     1021 tcggccgcca tggtgctggc aagcggggca acgcgcgaca tcgcgctgga ggcggccaac
     1081 gacgcgctcg cccgcatcag gattcggacc gcatgaacgc gccgtttccc agccggcaat
     1141 tcgatctggc gactggcacc ttgcggctgt tcgcgttctt tcatctcaat ctcgcttttt
     1201 cctcgatcga agaagagcgg cgcggcgagg tcatcgcgca atgctattgg ccgctgctca
     1261 ggctcgccga acggtgcggc ccggtcgggg tggaagcctc cggctacacg ctggaggaaa
     1321 tcgccaagct cgaccccgcc tggatcgagg cgctcaagcg tctgatcggg gcgggccaag
     1381 tggacttcat cggcgcgggc tacagccaga tgatcggtcc gctggttccc taccgcgtca
     1441 cccaagccaa tctcgccatc ggcaacgaaa cctatcggcg ccttttgggc atccggccgt
     1501 ccatcgcgct ggtcaacgaa caagcctatt cgggcggctt ggtgggcctt tacctcgacg
     1561 ccggttatcg caccttgctg atggattggg acaatccgtc gtcgcatcac caatggcccg
     1621 ccgaagcgcg ctatgcgccg cagcgcgccg ccggcgccga cgggcgcacc atcggcctca
     1681 tctggaccaa cacggtcgcc ttccagaagc ttcagcgctt cgcgcatgac gatatcggcc
     1741 tggacgatta tctcgcctat gtccgcgccc aacgcggaac ggtagcgcgc gcgctctgcc
     1801 tttacgccag cgatgcggag gttttcgatt tccgccccgg ccgcttccgc accgaggaaa
     1861 agaacaatgg cggcgagtgg gagcggctgg aacaagcgtt cgaacgcctg gcgcaagag
//
LOCUS       DASWPU010000314         4546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_113062,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000314 DASWPU010000000
VERSION     DASWPU010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113062"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /gene="pyrF"
                     /locus_tag="VGC27_06730"
     CDS             complement(<1..675)
                     /gene="pyrF"
                     /locus_tag="VGC27_06730"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY0282299.1"
                     /translation="MITARFANPLFVALDTPDLARALAIAKAVKPYVGGLKVGLEFIS
                     ALGPDAVRRIVEVGLPVFADAKFHDIPNTVAGAARAIAMLGVRMFNVHASGGEAMMRA
                     AAQAIADVHPRPLLIAVTVLTSLDDAALASVGQSGPSAAQAERLALLAKASGLDGVVC
                     SAHEVASVRRICGPDFLTVVPGIRPSGSAVEDQRRVMTPAKALAAGADVLVVGRPITA
                     VPDPAKA"
     gene            complement(887..1177)
                     /locus_tag="VGC27_06735"
     CDS             complement(887..1177)
                     /locus_tag="VGC27_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY0282300.1"
                     /translation="MIKSELVARLTARYPHLYHRDVERIVSTVLDNITKALADGHRVE
                     LRGFGAFSVKVRPSRMGRNPRTGEPVEVDEKRAPFFRTGKELRERLNGNGEA"
     gene            complement(1370..1585)
                     /locus_tag="VGC27_06740"
     CDS             complement(1370..1585)
                     /locus_tag="VGC27_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282301.1"
                     /translation="MKTNDRRLGPGDVRYANRGGQPGNRNALKTGAHIASMRAWRAWG
                     RDWRKRVKVALVQAEAEIRAPAPLPPP"
     gene            complement(1572..2000)
                     /locus_tag="VGC27_06745"
     CDS             complement(1572..2000)
                     /locus_tag="VGC27_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282302.1"
                     /translation="MNNPPNRADAGAAQAPAEAEILPPQAASAAQPLCTEALLDTLIE
                     ECRRLVREVATPSMAAMTDPLERRLFMQSAVSFIEAGAKVGDTVARLRSGGVQSETRH
                     RVFVEHVTPPLAPLRTQTRSLLEEDDEEEWGEGDRNHENE"
     gene            complement(2197..3354)
                     /locus_tag="VGC27_06750"
     CDS             complement(2197..3354)
                     /locus_tag="VGC27_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY0282303.1"
                     /translation="MARGVARKGLPLLDKTGQPFRYVLISDFLRALHEIDSNARGLVG
                     LPGGAATAEGRDVYLQKSLVEEPFSSSALEGAATTRAVAKKMIEEDRKPKTLDEKMVL
                     NNYRAMAFIREHRGEDLTPAMLLELHRILTVDTLERPEKVGVLRAPDDSVRVEETITG
                     DILHLPPPAADLPGRLDALCEFANAPSNGKGGFLHPVIRAIVLHFMLAYDHPFWDGNG
                     RCARALFYWCVLRHGYWLLEYVSISSVIRRAPKKYGMAFLYSETDEGDLTYFIRHQLD
                     VLETSLVDLQKYLAAKSRELDKLGKTMGALEQRLNRRQLPIIQDALKRETARYMIAEH
                     AKLHSVSYLTARSDLENLAKLGLLKKTKEGVLSVYLVPRDLKERLERLGKF"
     gene            3812..>4546
                     /locus_tag="VGC27_06755"
     CDS             3812..>4546
                     /locus_tag="VGC27_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY0282304.1"
                     /translation="MNDSQYIHRRSFLRRTGAAGAAVAAGPLVMGAAPKGSSVSLVID
                     GADPVAAAAPVQGAAKELRQALADAGFTVHRHERAEQAGKGELCIVASASAAPVAAAA
                     LNDAQISAPAVPESLALLSTSIAGRPAILACGADARGITYALLELADRVRHGAALKIA
                     TPIAERPANPVRSVMRQFVSEMYDKPWFYDREMWPRYLGMLAAQRFNRFQLAFGLGED
                     MLRHVQDQYFLFTYPFLMAVPGYDVRV"
BASE COUNT          870 a         1429 c         1416 g          831 t
ORIGIN      
        1 cgccttggca gggtccggca cggcggtgat cggccggcct acgaccagga catcggcccc
       61 cgccgcgagg gccttggccg gcgtcatcac ccggcgctga tcttcgacag cgctgccgga
      121 gggacggatg cctggaacca cggtcaggaa gtcggggccg caaatcctgc gcaccgaagc
      181 gacctcgtgg gcgctgcaaa ccaccccgtc caatccgctg gccttggcga gcagcgccag
      241 ccgctccgcc tgcgccgccg atgggccgga ctggccgacg gaggcaagcg ccgcatcgtc
      301 gaggctggtg agcaccgtca ccgcgatcag cagcggccgc ggatgcacat cggcaatcgc
      361 ttgcgccgcg gcgcgcatca tcgcttcgcc gccggaggca tgaacgttga acatgcgcac
      421 gccgagcatg gcgatggcgc gcgccgcgcc cgcaaccgta ttgggaatgt cgtggaactt
      481 ggcgtcggcg aagaccggaa ggccgacctc cacgatcctg cgcaccgcat cggggccgag
      541 tgcgctgatg aattcgagac cgactttcaa accgccgaca tagggcttca cggcctttgc
      601 gatggccagg gcgcgcgcca gatcaggcgt gtcgagggca acgaaaaggg gattggcaaa
      661 acgcgccgta atcatttcaa gcacctacca taaaactgaa ttcccgattt cgtcatggcc
      721 gggcttggcc cggccatcca gtgttttcgt tagcagtaga gcgggttcaa gactggatgg
      781 ccgcctcaag cgcggccatg acgaatgact gtgatcatcg tctcatccca aattatcccg
      841 gcaatacaga tcggcgcaac cgcaacgtgc agcactgcga gcagcgtcat gcttcgccgt
      901 tgccgttcag gcgctcgcgc aattccttgc ccgtgcggaa gaaaggcgca cgcttctcgt
      961 ccacctcgac cggctcaccc gtacgcggat tgcgccccat gcgcgagggg cgcaccttca
     1021 ccgaaaacgc gccgaagccg cgaagttcca cacgatggcc atcggccagc gccttggtga
     1081 tgttgtcgag aacagtcgag acgatccgct ccacatcgcg atggtagagg tgcggatagc
     1141 gtgccgtcag acgtgcaacc agttcggact tgatcatccc atgcccccga gaggacagct
     1201 tggccacgat attgccaaat gccgcctcgg caagggttaa ccctgtcaga acaaagccgg
     1261 gccgtcaacc ggaagtctct gatgaatcag cattttccac aaatgtgacg gaaatcactc
     1321 agccggggaa agccgggttt tggcccctcg cccggccaaa tcccgcgact caggggggcg
     1381 ggagaggcgc cggggcgcgt atctccgcct cggcctggac caacgccacc ttcacccgct
     1441 tgcgccaatc gcggccccag gcgcgccaag cccgcatcga ggcgatgtgc gcgccggtct
     1501 tgagggcatt gcgatttccg ggctggcccc cacggttagc gtagcgcaca tcgccagggc
     1561 ccaaccggcg gtcattcgtt ttcatgattc cgatccccct ccccccactc ctcctcatca
     1621 tcctcctcga gaagagagcg tgtctgggtg cgcagcggcg caagcggggg cgtaacgtgc
     1681 tcgacaaaca cgcgatggcg ggtttcgctt tgcaccccgc cggaacgcag gcgcgcgacc
     1741 gtatcgccga ccttggcgcc ggcctcgatg aacgaaacgg cggattgcat aaagagcctg
     1801 cgttccaagg ggtccgtcat cgcggccatc gacggcgtgg cgacttcgcg caccagacgg
     1861 cggcattcct cgatcagagt atcgagcaga gcctcggtgc agaggggctg ggccgcggaa
     1921 gcggcttggg gaggaaggat ttcggcctca gccggagcct gcgcggcacc agcgtcagcg
     1981 cggtttggag ggttgttcat gcgtgcgctt tcctatttcc acccgcccat tatcgcgccg
     2041 ttccggcggt gttggataag tgaggcgagg ttccggtcct ggcgcgcggt gaggcgcatc
     2101 agcccgttga accaattggg gaaaatcgcg cggacggaac gcggcggtcg caatggggat
     2161 gagggataag caaaaaccgt tcggctgcgc ggacggctaa aacttgccca ggcgttccag
     2221 ccgttccttc aggtcgcgcg gaacgaggta taccgacagg acgccttcct tcgtcttctt
     2281 aagaaggccc agcttcgcca agttttccag gtccgaacgc gccgtaaggt agctgaccga
     2341 atgaagcttg gcgtgctcag cgatcatgta acgtgccgtc tcgcgtttga gggcatcctg
     2401 gatgatgggg agttgacgcc ggttcagccg ctgttccaac gcgcccatcg tctttccgag
     2461 tttgtccaac tctcggctct tggcagcgag gtatttctgt agatctacca acgaggtttc
     2521 caggacatcg agctgatgcc ggatgaagta ggtcaggtca ccttcgtccg tctcgctgta
     2581 caaaaatgcc atgccgtatt tcttgggcgc gcgccggatg accgaagaga tcgaaacata
     2641 ttcgagcagc caatagccgt gcctaagcac acaccagtag aacagcgcgc gggcacagcg
     2701 cccattgccg tcccaaaacg gatgatcgta agccagcata aagtgcagca cgatcgcccg
     2761 aatgaccgga tgcagaaaac cgcctttgcc gtttgacggc gcgttggcga actcgcacaa
     2821 cgcgtcgagg cgcccgggaa ggtccgccgc cggcggtggc aagtgcagaa tatcaccggt
     2881 tatcgtctct tcgacgcgca cggagtcgtc cggcgcccgc agcacgccca ctttttccgg
     2941 ccgttcgagc gtatccaccg tcaggatacg atggagttcg agcagcatcg ccggcgtcaa
     3001 atcttcaccg cgatgttcgc ggatgaatgc catggcgcga tagttgttga gcaccatttt
     3061 ctcgtcgaga gttttgggct tgcgatcttc ctcgatcatc ttcttggcga cggcgcgcgt
     3121 cgtcgccgcg ccttccagcg ccgaactcga aaacggttct tcgacgagcg acttctgtaa
     3181 atagacgtcg cggccctcgg ccgtggccgc gccacccggc aggccgacca gcccgcgggc
     3241 attgctgtcg atctcatgca aggcgcggag gaagtcggaa atcagaacgt agcggaatgg
     3301 ctggccggtc ttgtccaata gaggcaggcc tttgcgcgct acgccccgcg ccattttcat
     3361 tgccgtccat aattcagcgg gcgtcagcgg tgggggcggc ttgcggaaac ggaccttctc
     3421 ccaatggtcg taggacgcgt ccggcgcagc attggctata ggccagaacg aatcccattt
     3481 cgctttgtcg cctgacatcg ttcggagaag atcgttatgg ttcggtggcg tagcgggcag
     3541 tttcatttta tagcctataa aataaaagtt gcaattccac aattatccgt ttgttttcaa
     3601 cctgttatat tttagacagg gctacccgag atggcgcagt tatatagggt ataaatttat
     3661 aacttataaa attgtagcgt gcaacgaaga aaatagacgc ggagcgagcc gcgccttgcc
     3721 tgttacaaat agcccgtggc ccccgtcacg gcgcggcggt aagtcaagtg ccacaagccc
     3781 cggcacatct atcgcggaaa gcccgatcaa aatgaacgac agccaataca tccaccgccg
     3841 cagttttctc cgccgcaccg gcgcagcagg agcggcggtt gccgccggac ctctggtcat
     3901 gggggcggcg ccgaagggaa gctccgtttc gctggtgatc gatggggccg atccggtcgc
     3961 cgcggcggcg cccgtccaag gggcggcgaa ggagttgcgg caagctctgg ccgatgcggg
     4021 tttcaccgtc caccggcacg aacgcgccga acaggcgggc aagggcgagc tttgcatcgt
     4081 tgcgtcggca agcgccgcgc cggtggcggc ggcggcgctg aatgacgcgc aaatttccgc
     4141 gcccgccgtg ccggagagct tggcgctgct ttcaaccagc attgccggac gcccggcgat
     4201 cctggcttgc ggcgccgatg cgcgcggcat cacctacgcc cttctggagc ttgcggaccg
     4261 cgtgcgccac ggcgccgcgc tgaagatcgc gacgcccatc gccgaacgcc cggcaaatcc
     4321 ggtgcgcagc gtcatgcgcc agttcgtcag cgagatgtac gacaagccct ggttctacga
     4381 ccgcgaaatg tggccgcgct atctcggcat gctggcggcg cagcgcttca accggttcca
     4441 gctcgccttc ggcctcggcg aggacatgct gcggcacgtg caggatcaat atttcctctt
     4501 cacctacccg ttcctgatgg cggtaccggg atacgacgtg cgggta
//
LOCUS       DASWPU010000315         6309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_106824,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000315 DASWPU010000000
VERSION     DASWPU010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106824"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..738
                     /locus_tag="VGC27_06760"
     CDS             316..738
                     /locus_tag="VGC27_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282305.1"
                     /translation="MTKIERKAKSRIAAGVSRRRLAVTFFAFFAFFIQSYIVQTHVHI
                     PAAAGTEWSGKLSAEAQKAAASLSQKGERGNYPADSDPSKCLLCQEFLASGSFVTPVA
                     AAAFVPLAFVGFTIAAQKSSPEDIAPSHHWQSRAPPRA"
     gene            1084..1497
                     /locus_tag="VGC27_06765"
     CDS             1084..1497
                     /locus_tag="VGC27_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282306.1"
                     /translation="MRKTYLGLLSALLLCPAALGASDPLVSDRSHESVYAARDALYVL
                     AHDGDRSRLSRIPSGTDAAQDVPLPYEERIAEISSDPRVAGITLVLESAAMPPRKFAY
                     IPRTSKFVHLKFGVRVGHEKAARFVELKRSARPDF"
     gene            complement(1576..3585)
                     /locus_tag="VGC27_06770"
     CDS             complement(1576..3585)
                     /locus_tag="VGC27_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0282307.1"
                     /translation="MIVTGRQSDKARLTDTSDTAALLSGLAGVATQSGGGLSSRPVIR
                     GMDDERIKIVVDGIEAASACANHMNPALSYASAASVDSIGLQAGVTPVSQGGDSLGGT
                     ITIKSAAPVFADDGAEPLVAGALGAFYRSNGANPGASANLTLATQNLSLRFDAALENA
                     SDYKDGGGRTVTSTYYKTRTLSGLAAYRDGDHLFTLRVGTQSVPGQGFANQQMDMIAN
                     NAIFGVLGYRGDFGWGALNAEIYGQNVRHLMNIGKDKIALAGAMMMPDMPMRTRGVDY
                     GARAGAEITLAKPGTLRLGADFHRTGLDDWWPPVDGTMMMAPDTFLNINGGRRDRFGV
                     YGELESRLSDRWTTLAGLRLDHVVTDTHDVAGYNAMYAAAAAAFNAVPHRRSDRDWDA
                     TLLARYTPNADDSYEFALARKTRAPGLYERYAWGTDWMSSEMINWAGDGNSYVGNQAL
                     KPELAYIASLSGLWRGAAGQGWQLKLTPYFTRVTDYIDVTAVKTRSKGLATMNLLQFV
                     NRSAALYGIDASFRAGLCHDDAFGHGEMRVEASYVHGRNIANHAPLYHIMPLNGRVTL
                     VQSIGGWENAVEIEDAARKSRVDPLRFEPQTPAYALLNLRTAYRWEKTRLELSITNLL
                     DTGYSLPLGGVDVDAYLGSNWTGAFTPLRGKGRSINIAISRDFSL"
     gene            complement(4149..>6309)
                     /locus_tag="VGC27_06775"
     CDS             complement(4149..>6309)
                     /locus_tag="VGC27_06775"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY0282308.1"
                     /translation="DKMKPDGTLDWTPPPGKWTVLRMGYSLTGSENHPATEEATGLEA
                     DKLNAGHVRSYMETYLDSYEAITGKELFGKRGINSVQVDSNEAGQQNWTEDILSEFKR
                     LRGYDAAPYLPVLTGVAVKSPAASDKFLWDFRHVISDLYDRNHYGVIAQVVRERGLFS
                     YAESLQSYRPAFDDGVVDRQFSEIPMGELWTYNKNYTKEYEFGNEADIMGAASLAHVY
                     GMNLVAAESLTSGEQPWNFAPRELKMFLDKELLRGVNRIVLHTSVHQPMDKAPGMSLG
                     RYGQFFTRHESWAEEAKPWVSYMARASYLLQQGRFVADIAYFYGQEAAPARVWGSKRV
                     SDVPPGYVFDFVDIDALLHQMSVEDGVIVTKSGMRYRVLYLGGSSNIMTLAAVNRIAE
                     MVRQGAVLVGEPPVDTPTLADGSAEFHAAVQDLFGDGTVHAYGKGKVFPAGSLSEALA
                     AINLPPDFAYSDGDARLLYLHRHLKDGEIYFVSNREDKAANLDATFRVAGKLPEIWDA
                     VSGKTAPATYRMENGRTTVALSLPPYGSAFVVFRKTAKVESVTLRKPVETAVKTIEGP
                     WTVAFQPDRGAPPKIELNSLTSWSDNADPGVKYFSGAGTYTKSFELPETAFKPEARLM
                     LDLGEVRELAEVSLNGKPVGTVWTTPFQLDITKAAKPGANVLKVKVVNLWVNRLIGDK
                     QPGVTKKYTFTHIPTYKPDAPLRESGLLGPVSILRVD"
BASE COUNT         1144 a         1947 c         2000 g         1218 t
ORIGIN      
        1 cgtttcccct gctgatcgat ccgttgaaaa tcaccctcgc gcgttacaag gtcagctcgg
       61 cgccggtgtt tttcatcgtg gacccgcagg gcaagctggt cgaacggatc gatggtccgc
      121 tcgatgaaaa actcctgaag gcgaaactta gccccttgct gtgaggcgcc gaaagatgcc
      181 ctttgggaac ggaaaaacca gccgaatgcc cgcccgtgcc tgaggtcaca gcggcgtgaa
      241 gatcgccaaa gcatcctaag ctaggcaaaa gccggcccgc aatgttaggc tgcggcgatc
      301 ccccgcggga cggcgatgac aaaaatcgaa cgcaaagcga aaagcaggat agcggccggg
      361 gtctcccgcc ggcggcttgc cgttacgttt ttcgcgtttt ttgcgttctt catccaaagt
      421 tatatcgtcc agacgcacgt ccatattcct gcggccgcag ggacggaatg gagcggcaag
      481 ctatccgccg aggcgcagaa ggccgccgcc tcgctttcgc aaaagggcga gcgcggcaat
      541 tatccggccg acagcgatcc gtccaaatgt cttttgtgcc aggaattcct ggctagcggc
      601 agcttcgtca ccccggttgc cgcggcagcc ttcgtgcccc tggcgtttgt cggtttcacc
      661 atcgcggcgc aaaaatcgtc tcccgaagac atcgcgccct cgcatcattg gcagagccgc
      721 gcacccccac gcgcctgaca tcacatcttc gtccggacgg ccacagttcg catgggcatc
      781 ccccgcgctg aggacgtccg catgttgctg gccgacagga acaggagctt tctcataacc
      841 gccggtgccg acacgtttcc tgccgaggac aacgggcgcg ttcgcccgtg gcgctcttgt
      901 ctgtcctcgc ctaaagcgct tcccgaaaag tggaaaccgg ttttcggata agaagcgcga
      961 caagacaaat agctggagca aatcaccgat tccagtaaac gatgatttgc tctaggcgca
     1021 ggcagaatgc gaagcggcca gatcatttca gtatccggaa catcttcgaa tatggagtat
     1081 tccatgcgta aaacctatct tggcctctta tccgcgctgc tgttgtgccc cgccgcgctc
     1141 ggcgcatccg atccgctcgt gtcggatcgc tctcacgaat ccgtctatgc ggcgcgcgac
     1201 gcgctgtatg ttctggcgca cgatggggac cgttcgcggc tgtcgcgcat tcccagcgga
     1261 accgatgcgg cgcaagacgt ccccttgcca tatgaggagc gcatcgccga aatatccagc
     1321 gatccgcggg ttgccggcat caccctggtg ctggaaagcg ccgccatgcc gccgcggaaa
     1381 ttcgcctata tcccgcgtac gagcaaattc gtgcatctga agttcggcgt ccgcgtcggc
     1441 catgaaaagg cggcaaggtt cgtggaactg aagcgcagcg cccgtccgga tttctgactc
     1501 gaaatcgccc tgtcgccaag gcgagcgcgc cgtgttatcg agccgcttgg ccttggcgtt
     1561 cggatttgtg ccggtctaca gtgagaagtc tcggctgatc gcgatgttga tcgaacggcc
     1621 cttgcccctg agcggcgtga acgcgcccgt ccagttgctg ccgagatagg catcgacatc
     1681 gaccccgccc agcggcagcg aatagccggt gtcgagcaga ttggtgatgg aaagctcgag
     1741 gcgcgtcttc tcccagcgat aggccgtgcg caggttgagc agcgcatagg cgggagtctg
     1801 aggctcgaag cgcagcggat cgacccggct cttgcgcgcc gcgtcctcga tttccacggc
     1861 gttttcccag ccgccgatgc tctgcaccag ggtcacgcgc ccgttgagcg gcatgatgtg
     1921 atagaggggc gcatggttcg cgatgttccg gccatggaca taggatgcct cgacacgcat
     1981 ctcgccgtgg ccgaaggcgt cgtcatgaca cagcccggcc cggaaggagg cgtcgatccc
     2041 gtagagcgct gcgctgcggt tgacgaactg caacagattc atcgttgcca gccccttgct
     2101 gcgggtcttc acggcggtca cgtcgatata gtcggtgacg cgggtgaaat agggcgtcag
     2161 cttcaattgc cagccttgtc cggcagcgcc gcgccacagg ccggagaggc tcgcgatgta
     2221 ggccagctcg ggcttgaggg cctggttgcc gacataggaa ttgccgtcgc ccgcccagtt
     2281 gatcatttcc gacgacatcc agtcggttcc ccaggcatag cgttcgtata ggcccggcgc
     2341 ccgcgtcttg cgcgccagag cgaattcgta gctgtcgtcc gcattaggcg tgtagcgggc
     2401 gagcaaggtc gcgtcccaat cgcggtcgct gcgccggtgc ggcacggcgt tgaacgcagc
     2461 cgcggcggcg gcatacatcg cgttgtagcc cgcaacatcg tgggtgtcgg tgacgacatg
     2521 gtccaggcgc aggccggcca gcgtcgtcca acggtcggac agcctgcttt ccagctcgcc
     2581 atagacaccg aaacggtcgc ggcggccgcc gttgatgttc aggaaggtat cgggcgccat
     2641 catcatggtg ccgtccaccg gcggccacca atcgtccagg ccggtgcgat gaaaatccgc
     2701 gcccaggcgc agtgtcccag gtttcgcaag cgtgatttcc gcaccggccc tggcaccgta
     2761 atcgacgccg cgggtccgca tcggcatgtc cggcatcatc atggcgccgg ccagcgcgat
     2821 cttgtccttg ccgatgttca tcagatggcg gacgttctgg ccgtagattt ctgcgttcag
     2881 cgcgccccag ccgaaatcgc cccggtatcc cagaacgccg aagatcgcgt tgtttgcgat
     2941 catgtccatc tgctggttgg cgaagccctg gccggggacg gactgggttc caacacgcaa
     3001 ggtgaacaga tgatcgccgt cgcgataggc ggccagcccc gacagggtgc gcgtcttgta
     3061 ataggtcgat gttacggtcc ggccgccgcc atccttgtag tcgctggcgt tttccagcgc
     3121 ggcgtcgaag cgaaggctga ggttttgcgt ggccaaggtg aggttggccg aggcgccggg
     3181 gttggcgcca ttgctgcgat agaaggcgcc gagcgcgcct gcgaccagcg gctccgctcc
     3241 atcgtccgcg aaaaccggcg cggcggattt gatcgtgatc gtgccgccca ggctgtcgcc
     3301 cccttggctg accggtgtga cgccggcctg aagcccgatg gaatcaacgg aggcggcgga
     3361 ggcatagctg agcgccggat tcatgtggtt ggcgcaggcc gaggcggcct cgatgccgtc
     3421 caccacgatc ttgatccgct catcatccat cccgcgaatg accggccggc tcgacaaccc
     3481 gccgccgctt tgcgtggcca cgccggcaag gccggacagc agcgcggcgg tgtcgctggt
     3541 gtccgtcagc cgggctttgt ctgactgccg gccggtgacg atcacggttt ccggcgctca
     3601 gtcatatgcg gcgaaggcgg cgggggcagc gaccagggga aaaagggcgg aagaaaacaa
     3661 caggcgcgat ctgctggaca tggcaacccc gggaagaaat tgggccgaaa gaggaagctg
     3721 gtagttacaa aatttcgcgg cggcctgacg cgccacttgc aagcacgtca attgcggatg
     3781 agacctaatc tcataaacaa gtacgctttg gggcccatag tataaatgtg gaaaattgac
     3841 ccaccggcgt ctttccgaat gccggcggaa agacgaaccg gattaccggg aacccttctt
     3901 ggccatcggg actttgagga tcactacact tgcggcatgg agaaggcgga ggcgcgccgc
     3961 agcgatcgtt ctcttctggg gagttccgat ctcccggccg ccagggacat tgtgcgcgga
     4021 gccgatcgcc ccaatgaatg gcgacatcct tcacttcagg tcctgccatt gctgccgggc
     4081 acagcagcaa tggcagtaag tttttccctg tgtgttcttc ttatttgtct ttcttgttgc
     4141 cgctccgatc agtccacgcg caggatcgac accggcccca acagacccga ttcccgcaag
     4201 ggagcatccg gcttgtaagt cgggatgtgg gtgaaggtgt acttcttcgt cacacccggt
     4261 tgcttgtcgc cgatcagccg gttgacccac agattgacca ccttgacttt caacacattc
     4321 gcgcccggct tcgcggcctt ggtgatgtca agctggaacg gcgtcgtcca gacggtgccg
     4381 accggcttgc cgttgaggct cacctccgca agctcgcgaa cctcgccgag atcgagcatc
     4441 aggcgcgctt caggcttgaa cgcggtttcg ggaagttcga acgacttggt gtaagtgccc
     4501 gcgcccgaga agtacttcac gccgggatcg gcattatcac tccacgacgt caggctgttc
     4561 agctcgatct tgggcggagc gccgcgatcc ggctggaagg cgacggtcca gggcccttcg
     4621 atggtcttga ccgcggtttc caccggtttg cgcagtgtca ccgattccac cttggccgtt
     4681 ttgcggaaga ccacgaaggc cgagccgtag ggcggcaggg acaaagcgac cgtggtgcgg
     4741 ccgttttcca tgcgataggt ggcgggcgcc gtttttccgc tgacggcgtc ccagatttcg
     4801 ggcagcttgc ccgcgacgcg gaaggtggcg tcgaggttcg ccgctttgtc ttcgcggttg
     4861 ctgacgaaat aaatctcgcc gtccttcaga tggcgatgca ggtaaagcag tcttgcatcg
     4921 ccgtcggaat acgcgaagtc gggcggcagg tttatggcgg caagagcctc gctgagcgaa
     4981 ccggcgggga acaccttgcc cttgccgtaa gcatgcaccg tgccgtcgcc gaagaggtcc
     5041 tgcacggcgg catggaactc ggcgctgccg tcggcgaggg taggcgtgtc caccggcggt
     5101 tcgcccacca gcacggcgcc ctggcgcacc atctccgcaa tgcggttcac ggcggcgagc
     5161 gtcatgatgt tgctggagcc gccgagatag agcacgcgat agcgcatccc ggatttggtc
     5221 acgatcacgc cgtcttccac cgacatctga tggagaagcg catcgatatc gacgaaatcg
     5281 aagacatagc ccggaggtac gtcggagacg cgcttgctgc cccagacgcg cgccggcgcg
     5341 gcttcctggc cgtagaaata ggcgatgtcg gcgacgaaac ggccctgctg cagaagatag
     5401 gaggcgcgcg ccatgtacga cacccagggc ttcgcctcct ccgcccagct ttcatgccgc
     5461 gtgaagaact ggccgtaacg gccgagcgac atcccgggcg ccttgtccat aggctggtgc
     5521 accgaggtgt ggagcacgat gcggttgacg ccgcgcagaa gctccttgtc gaggaacatc
     5581 ttcaattcgc gcggagcgaa attccagggc tgttcccccg aggtgaggga ttcggcggcc
     5641 accagattca tcccatagac gtgcgccagc gaggcggcgc ccatgatgtc ggcttcgttt
     5701 ccgaactcgt actccttggt atagttcttg ttgtaggtcc acaactctcc catcggtatt
     5761 tcggagaact gccggtccac cacgccgtcg tcgaaggcgg ggcggtagga ttgcagggat
     5821 tccgcatagc tgaacaagcc gcgctcgcgc accacctgcg cgatcacgcc gtaatggttg
     5881 cggtcgtaga ggtcgctgat gacgtgccga aaatcccaca ggaacttgtc gctggcggcg
     5941 gggcttttca ccgccacgcc ggtgaggacg ggcaggtagg gcgcagcatc atagccgcgc
     6001 aggcgcttga actcggagag gatgtcctcg gtccagttct gctgcccggc ttcgttgctg
     6061 tcgacctgga cggagttgat gccgcgcttg ccgaacagct ccttgccggt gatggcctcg
     6121 tagctgtcga gataggtctc catgtaggaa cgcacatggc cggcgttgag cttgtcggct
     6181 tccagccccg tggcttcctc cgtggccgga tggttttccg agccggtcag cgaatagccc
     6241 atgcgcagca ccgtccattt gcccggcggg ggcgtccagt cgagcgtgcc gtcgggcttc
     6301 atcttgtcc
//
LOCUS       DASWPU010000316         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_39810,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000316 DASWPU010000000
VERSION     DASWPU010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39810"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1033)
                     /locus_tag="VGC27_06780"
     CDS             complement(116..1033)
                     /locus_tag="VGC27_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0282309.1"
                     /translation="MDSLLNAAPGLGHSGTGNNMRSVHGWIVTILCALIVLARPVFAA
                     PQRVVSTFLCTDEYVFRLVPHQRIAALSYEATDRNPVVSTIADRARGIRTIHPTTETV
                     LSLAPDLVVMYAGTNPRLHLNLKKLGIAVLDVPWANSLADVRRITVMLGEKLGAPGKA
                     KEMLAVMDRKIASANAAAPRPPVRTILFEPNGYASTGGITDEVMALSGIANAAPQAWL
                     TRMGTLPVEAVIAAAPELLILGGEERSGSARAYAILHHPALMALEGKTYMEHAVLTPL
                     LCPGPWSLDAAGTFGDLARKARALAPAKK"
     gene            1032..>1831
                     /locus_tag="VGC27_06785"
     CDS             1032..>1831
                     /locus_tag="VGC27_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282310.1"
                     /translation="MPRAYTAILVAAFVATISYSSPAFAASSAGQILDAYKAATGGSA
                     WDNKVTMSGEITISGYGLTGVGHIIADLRNGRTMTDYMMGPAGGANGFDGATPWQKDM
                     SGTVTLQQGGDALMLAVNDAYRTAGKWWQPDRGGAEIVGAGEKTEGGATYEVLTITPK
                     GGKPFDAWFDAKTHLLAKTVEKQGSQTVTVTLSDYRRTDGVLLPYKQVTDAGVGEKYL
                     RTITVTSVAFLGPQPDSAYAPPKVTITDFSIAGGAAQTKFPIQLINNH"
BASE COUNT          368 a          581 c          577 g          305 t
ORIGIN      
        1 cgagcgaccg cagggagcat gcctacctaa cacctgggtc cccttccctc gcacgaacct
       61 agcggttcgt gctcgccggg gatgacaccg caatttgatc tgaaaagcct gcgtcctact
      121 tcttcgccgg tgcaagcgcg cgagccttgc gcgccagatc gccgaaggtt ccggccgcgt
      181 ccagcgacca ggggccgggg cagagcagcg gcgtcagcac cgcatgttcc atataagtct
      241 tgccttccaa cgccatgagc gcgggatggt gcaggatcgc gtaagcccgc gccgagccgc
      301 tgcgctcctc cccgccgagg atgagaagtt cgggcgcggc cgcgatcacc gcctcgaccg
      361 gcaaggttcc catacgggtg agccaagcct gcggcgcggc gttggcgatc cccgacagcg
      421 ccatcacctc gtcggtgatg ccgccggtgc tggcataacc attgggctcg aaaagaatcg
      481 ttctcacagg cggacgcggc gcggcggcgt tggcggaggc gatcttgcgg tccattacgg
      541 cgagcatctc ttttgccttg ccgggcgcgc cgagcttttc gcccagcata accgtgatcc
      601 tgcgcacgtc ggcgagcgag ttcgcccacg gcacgtcgag gacggcgatc ccgagcttct
      661 tcagattgag atgcaggcgg gggttggtgc ccgcatacat caccaccaga tcgggggcga
      721 gagagagcac cgtttccgtg gtgggatgga tcgtgcgaat gccccgggcg cggtcggcga
      781 tggtcgagac caccggattt cggtccgtcg cctcgtagga cagcgccgcg atccgttggt
      841 gcggaaccag ccggaagaca tattcgtccg tacataaaaa cgtggacaca acccgttgcg
      901 gcgctgcaaa taccggccgc gcaaggacga tcagcgcaca caaaatcgtg acaatccagc
      961 catgcaccga gcgcatattg ttgccggtgc cggaatgccc caatcctgga gccgcattca
     1021 ggagagaatc catgccgaga gcctataccg caattctcgt tgccgcgttc gtcgcgacca
     1081 tatcctattc gtcgccggcg ttcgccgcat catcggccgg acaaatcctc gatgcctata
     1141 aggccgcaac cggcggcagc gcttgggaca ataaagtcac catgagcggc gagatcacga
     1201 tctccggcta tggcctgacc ggtgtgggac atatcattgc cgatctcagg aacggccgca
     1261 ccatgaccga ttacatgatg ggtcccgcag gcggcgccaa cggcttcgac ggcgctaccc
     1321 cttggcagaa ggacatgtcg gggaccgtca cgctgcaaca gggcggcgac gcgctgatgc
     1381 tggcggtcaa cgacgcctac agaaccgccg gcaaatggtg gcagcccgat cgcggcggcg
     1441 ccgagatcgt gggcgcgggc gaaaagaccg aaggcggcgc gacctatgag gtgctcacca
     1501 tcacgccgaa gggcggcaag ccgttcgacg cctggtttga cgccaagacg catctgctgg
     1561 cgaagacggt cgagaaacaa ggctctcaaa cggtgaccgt aacattgtcg gattaccggc
     1621 gcaccgatgg cgtattgttg ccctataagc aggtgaccga tgcgggcgta ggcgagaaat
     1681 acctccggac gataacggtg accagtgtgg cgttcctcgg tccgcaaccg gactcggcct
     1741 atgcgccgcc caaggtgacg atcacggatt tctcgatcgc cggaggcgcg gcacaaacga
     1801 aatttccgat ccagctcatc aacaaccaca t
//
LOCUS       DASWPU010000317         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_51645,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000317 DASWPU010000000
VERSION     DASWPU010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51645"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..943)
                     /locus_tag="VGC27_06790"
     CDS             complement(176..943)
                     /locus_tag="VGC27_06790"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0282311.1"
                     /translation="MTLLAKAKRTVGALLGRYYPRGLIFYHLRTRSRNCEPELWLVPK
                     LVNRAGLAVDVGANEGYWSLQLVRYAKHVHAFEPNPICLAQLRRVLPRRVALHEVALS
                     DSSGTKSLRFDPNNTGIGTIESGNPLDNNVGIKQIETRDVSTTCLDDFQLNGVELIKI
                     DVEGHEEAVLRGAAATLERNRPTVICEIEERHNPGGLKRIRESFERRNYRAYAIDGDV
                     VRPLAAIEAEGRTKLGVASGINNFIFADEKLALALFS"
     gene            complement(940..1647)
                     /locus_tag="VGC27_06795"
     CDS             complement(940..1647)
                     /locus_tag="VGC27_06795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0282312.1"
                     /translation="MKLFFSAIKRIPGLYEFLRYRVYAPLQSKNRKRVFLKIYRENAW
                     GDRSSVSGPGSSLETTAELRSVLPGLLADLQVKSLLDLPCGDLEWIKHVSLGIEKYIG
                     ADIVEPLIVRNRAQYADFGEFLRLDLLTDALPAVDAIFCRDCLIHLSNREIMKALGNI
                     ARTNAQYLITTTFPDLSANKDTVTPYWRAVNLQLPPYNLPPPVRLVRDYSPLQPDDEQ
                     KYLGVWRIGDLRGISSI"
     gene            complement(1700..2806)
                     /locus_tag="VGC27_06800"
     CDS             complement(1700..2806)
                     /locus_tag="VGC27_06800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0282313.1"
                     /translation="MIVSLSRYLHDRDWDHTIVCFGQTIDFAAHAAWPMRLHELRPAR
                     NAISEGRALSRYFQNVYVAGGSLPLFFDLKSAFYAGLFRCPGFHLHLTDPPSLLPADV
                     SKCAPSLRKAYPGNSPAEEPGVLLKIRGELVHQINRRGVKRAKSLITMANANANELRA
                     LYGRQAAVIRQGVAPPSLKPRLAGRRLKPFRLLSVSRLEKNKRLDWVFEALAKLEASD
                     VPFSSRCDWVLDVVGDGSQAAPLRGLARRLGIESRVAFHGRASDEALDALFAGAGLFL
                     MPAIQGYGLPALEALCREVPVILHRESGVSEILGQSPWVAVIDHGPDDLALAIRTMVG
                     NISSGMVEKHPVPRFPTDTEWAEEIAKACGWVGG"
     gene            complement(2913..>3218)
                     /locus_tag="VGC27_06805"
     CDS             complement(2913..>3218)
                     /locus_tag="VGC27_06805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0282314.1"
                     /translation="ADMLFFPSRQEWLGMVAVEAQAAGLPVLASDAVPAAAKVVPELY
                     RSLPVHSSLADWTDALFATLNTPRVAMDAARRAVEASDFSIETSASRLARIYGGHGH"
BASE COUNT          696 a          990 c          914 g          618 t
ORIGIN      
        1 accgagccca attccaaacc cgtcaggtaa gagaacaagc caaagcgccg gtcaagccag
       61 cgcatgacca ggaagcgcag cggcacaggc tctctgagcg tttctcgaag ttctatgagc
      121 atctcatgtt atcctgggaa cgcattcgaa gcgttactgg cgcggacggg actgttcagg
      181 aaaatagagc caacgccaac ttttcgtccg caaaaatgaa gttgtttatc cccgaggcga
      241 cgccgagttt cgttctcccc tcggcctcga ttgccgccaa cggccgaacc acatcgccgt
      301 ctatcgcata ggctcgataa ttgcgccgtt cgaaggattc acgaatcctc ttcaagccgc
      361 ccgggttgtg ccgttcctcg atttcgcaaa tgacggtcgg acggttgcgc tccaacgtgg
      421 cggcggctcc gcgaagaacg gcctcctcat gaccttctac gtcgatcttt atcaactcga
      481 cgccgttgag ttgaaaatcg tcgaggcacg ttgtcgagac atccctcgtc tcgatctgct
      541 tgatacccac gttgttgtcc aggggatttc cggattcgat ggtgccgatc cccgtattgt
      601 tcgggtcgaa gcgcaggctt ttcgtgcccg agctgtcgga aagagccacc tcgtgcagcg
      661 cgacccgtcg cggtaaaacg cgccgcaatt gcgccaggca gatcgggttc ggttcgaagg
      721 catgcacatg ctttgcatat ctgacgagct gaagggacca atagccctcg tttgcgccga
      781 catcgaccgc caatccagcg cggtttacca attttggaac cagccaaagt tcgggttcgc
      841 aattccggct tcgcgtgcgc aaatggtaga agatgaggcc gcgcggatag taccggccga
      901 gcaacgcccc gaccgtacgc tttgctttgg cgagaagcgt catatcgaag atattccgcg
      961 aagatcgccg atccgccaga cgcccaggta tttctgctcg tcgtcgggtt gcaagggcga
     1021 ataatccctg acaaggcgca cgggcggggg aaggttgtaa ggaggcagct gaaggttgac
     1081 cgcgcgccaa tagggcgtga cggtgtcttt gttggcgctc aggtcgggga acgtcgtggt
     1141 aatcaggtac tgtgcgttcg tccgcgcgat attccccagg gccttcatga tttcccggtt
     1201 gctgagatgg atgaggcagt cccggcaaaa aattgcgtct accgcgggca aggcatcggt
     1261 gagcagatcg aggcgcagga attcgccgaa atccgcgtat tgcgcgcggt tccgtacgat
     1321 caacggctcg acgatgtccg ccccgatata tttctcgatt cccagagaga cgtgttttat
     1381 ccactccaaa tcgccgcacg gcagatccaa cagcgatttc acctgaagat ccgccagcaa
     1441 gccgggcagc acgctgcgaa gttcggcggt cgtttcgagc gacgatccgg gtccggaaac
     1501 ggacgaccgg tcgccccatg cgttctcccg gtatattttc aggaagacac gcttcctgtt
     1561 tttcgactga agcggcgcat atacgcgata acgcagaaat tcgtacagac ccggaatgcg
     1621 ttttatggcg gaaaaaaaca atttcattct ccctcgtgac gatatcggac ggcgccgggc
     1681 ggtggcgctc aaaacagctt caaccgccca cccagccgca cgccttcgcg atctcctcgg
     1741 cccattccgt atccgtcgga aaccggggaa cggggtgttt ctcgaccatg ccgctggaga
     1801 tattgcccac cattgtcctg atcgccaagg ccagatcgtc cggcccatga tcgatgacgg
     1861 cgacccatgg cgattgcccc agaatttcgg aaacgcccga ctctcgatgc aggataacgg
     1921 ggacctcgcg acaaagagct tccaaagccg gcaatccgta cccttggatc gccggcatca
     1981 ggaagaggcc ggccccggca aacagcgcat ccaacgcctc gtccgaggct ctgccgtgaa
     2041 atgccaccct cgattcaatt cccaaccgcc gggcaaggcc ccgcaaaggg gcggcttgtg
     2101 atccgtctcc aacgacgtcc agaacccagt cgcaccgcga actgaaagga acatcggacg
     2161 cctccagttt cgccaaggct tcgaataccc aatcaagccg tttgtttttc tccagcctgg
     2221 aaaccgaaag cagccggaat ggcttgagcc tgcgccccgc cagacgaggc ttgagcgatg
     2281 gaggcgccac gccttggcga atgaccgcgg cctgcctccc gtagagagcc ctcaattcgt
     2341 tcgcattagc gttcgccatc gtaatcaagg atttggcacg cttgacgccg cgccgattga
     2401 tctgatgcac caattcgcct ctgatcttca gcaagacccc cggctcctcg gccggcgaat
     2461 tccccggata tgctttgcga agcgagggag cgcacttgct gacatcggcg ggcagcaggc
     2521 tgggcggatc ggtcaggtgc agatgaaagc ccggacagcg gaataacccc gcgtagaacg
     2581 cgcttttcag atcgaagaac agcggcagcg atcctccagc gacataaacg ttttggaaat
     2641 aacggctcaa ggcccgtcct tcggagatgg cattgcgcgc cggccgcagc tcatgcaggc
     2701 gcatgggcca cgccgcgtga gcggcaaaat cgatcgtttg gccgaagcag acgattgtgt
     2761 gatcccaatc gcgatcgtgc agatagcgcg acaaggaaac gatcaacctc tccacgcctc
     2821 cgaaggcgtg gatataggga acaaaaatgg caattcgccc agcagatacc ctattcaacg
     2881 agcgctctcg atcttgccct gctcgagatt catcaatggc cgtgcccgcc gtaaattcgc
     2941 gccagccggc tcgccgaggt ttcgatggag aagtccgatg cctccaccgc cctgcgtgcc
     3001 gcgtccatcg cgacgcgcgg cgtgttcagc gttgcgaaca gcgcatcggt ccaatcggca
     3061 agggaggagt gaaccggcaa ggaccgataa agctccggca cgaccttcgc cgctgcaggc
     3121 accgcatcgc ttgccagcac gggcaatccc gccgcctggg cctccacggc caccatccct
     3181 aaccattctt ggcgcgacgg aaagaaaagc atgtcggc
//
LOCUS       DASWPU010000318         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_2421, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000318 DASWPU010000000
VERSION     DASWPU010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2421"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VGC27_06810"
     CDS             <1..1080
                     /locus_tag="VGC27_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEY0282315.1"
                     /translation="GRGRFGLALPVRLPKGEIWLQMTKVTRLTLDEDGGLDRRHIHLR
                     EISGDLDAPLETIGQDLRAARLRRGDDLATVSRVLKIRKDHLEALEEDRMDALPGRTY
                     AVGFTRSYADYLGLDAVQCVERLKAEIAGRSDETTPPITVIDEDEHRRLPQGWKIIAG
                     VVLLLVLYGAYQLAASADRMLNEPVTAAPPQMMASRPAVAAPPIRQPSEPIVVQQPLL
                     APPGDAGALPPAQTSSLPQAAVTDTAQLQSSGVSEPLPRGQVFGEHNRNARVVLRMRQ
                     AARILVQGPDGMVFINRALKAGDAYQVPNLVGATLTTANAGAVELDLDGVTIGPAGRN
                     NETAEALSLDPQAIMDRRNSREYGR"
     gene            1103..2230
                     /gene="ispG"
                     /locus_tag="VGC27_06815"
                     /gene_synonym="gcpE"
     CDS             1103..2230
                     /gene="ispG"
                     /locus_tag="VGC27_06815"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY0282316.1"
                     /translation="MSVRAYRDIYRRKSRQIHVGPVAVGGGAQISVQTMTNTVTSDAR
                     ATINQIRHIEEAGADIVRVSCPDEESTAAMKAICKASRIPVVADIHFHYKRAIEAAEA
                     GAACLRINPGNIGSAQRVKDVVKAAKDHGCSMRIGVNAGSLEKELLERYGEPCPEAMV
                     ESALSHAKILDDNDFREYKISVKASDPFLAVAAYRALAEATDAPLHLGITEAGGMISG
                     TVKSSIGMGALLWSGVGDTIRVSLSADPVEEVRVGFDILKALDLRHRGVNIISCPSCA
                     RQGFDVIETVRVLEDRLKHITTPMSLSIIGCVVNGPGEARETDLGFTGGGAGKGHGQI
                     YLNGEPAHRIDNKDLIEHIVELCENKAAEIEASRNKLEAAQ"
     gene            2327..3409
                     /gene="prfA"
                     /locus_tag="VGC27_06820"
     CDS             2327..3409
                     /gene="prfA"
                     /locus_tag="VGC27_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY0282317.1"
                     /translation="MIPTAKLERLLDRFHAVEAELASGVAGGTYVKLSKEHAELSPII
                     AAVEAYRAAEKQLGEADALVNDPSTDSEMRGLAEQERGELKALLERLERDLKLKLLPK
                     DAADSSSAILEIRAGTGGDEAALFAGDLLKMYTRYCQLQGWKTDLMSLSESDLGGIKD
                     ATLEVDGTGVFAKLKFESGVHRVQRVPVTEASGRIHTSAATVAVLPEPEDVDVVINEK
                     DLRIDVYRAQGAGGQHVNKTESAVRITHLPTGIAVAQQTEKSQHKNKAQAMKMLKAKL
                     FDMERERIDKQRAGERKSLVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLDRIISGD
                     DLGEIIEALVTEDQASRLAELEADGA"
BASE COUNT          717 a         1154 c         1184 g          596 t
ORIGIN      
        1 gggcgcggcc gtttcggttt ggcgctccct gttcgtttgc ccaaaggcga aatctggttg
       61 cagatgacaa aggttacgcg gctgacactg gatgaggatg gcgggcttga tcgccgtcac
      121 attcatcttc gggagatttc cggcgatctc gatgcgccgc tggagacgat cggccaggac
      181 ttgcgcgcgg cgcggcttag gcgcggcgac gacttggcga ccgtctcgcg ggtgctgaag
      241 attcgcaagg accatctcga agcgctcgaa gaggaccgca tggacgcctt gcccgggcgc
      301 acctacgccg tgggcttcac ccgttcgtat gcggattatc tcggactgga cgccgtacaa
      361 tgcgtcgagc gcctcaaggc ggagatcgca gggcgttccg acgagacgac acctccgata
      421 accgttatcg acgaggatga acatcgccgt ctgccgcaag gctggaagat catcgccggg
      481 gtggtgctgt tgctggtgct ctacggcgcg tatcagcttg ccgcttcggc ggatcgcatg
      541 ctcaacgagc cggtgactgc ggcgccgccg caaatgatgg cctccaggcc ggccgtcgcg
      601 gcgcccccga tacgccagcc ctccgaaccg attgtcgtgc aacagccgct gctggcaccc
      661 cccggcgatg caggcgcgct gccgcccgct caaacatcga gtctaccgca agcggcggtg
      721 acggacaccg ctcaactaca gtcttcaggc gtatccgagc ctctgccgcg gggccaagtc
      781 tttggcgagc ataacaggaa cgcgcgcgtc gttttgcgca tgcgccaggc ggccaggatt
      841 ctggtccaag gccccgacgg catggtcttc atcaaccgtg cgctgaaagc cggcgacgct
      901 tatcaagtac ccaatctcgt cggagcgaca ttgacgacgg cgaacgcggg agcggtggaa
      961 ctcgatctcg atggggtcac gatcggcccg gccggcagga acaacgaaac tgccgaagcg
     1021 ttgtcgctcg atccgcaggc gatcatggat cgtcgaaaca gccgcgaata cggcagatga
     1081 agaccatcgg cgaaaggttg ctatgagcgt ccgtgcgtat cgcgacattt accggcgtaa
     1141 atcccggcaa attcacgtag gtcccgtggc cgtcggcggc ggcgctcaga tcagcgtcca
     1201 gaccatgacc aataccgtca cttcggacgc gcgtgccacg atcaaccaga tccgccatat
     1261 cgaggaggcc ggggccgaca tcgtccgcgt ctcttgtccc gacgaggagt ccacggcggc
     1321 gatgaaggcg atttgcaagg cctcgcgcat tcccgtcgtg gcggacatcc atttccacta
     1381 caagcgcgcc atcgaggcgg cggaagcggg cgcagcctgc ttgcgcatca accccggcaa
     1441 tatcggcagc gcccagcgcg tgaaagatgt cgtgaaggcc gccaaggatc acggctgctc
     1501 gatgcgcatc ggcgtcaatg ccggctcgct tgaaaaggaa ctgctggagc gctatggcga
     1561 gccgtgcccc gaggcgatgg tcgaaagcgc tctcagccac gccaagattc tcgacgacaa
     1621 cgacttccgc gaatacaaga tttcggtgaa agcctccgat ccgttcttgg cggtggcggc
     1681 gtatcgtgcc ttggccgagg ccaccgacgc cccgctgcat ctgggcatca ccgaggcggg
     1741 cggcatgatc tccggcacgg tgaaatcctc catcggcatg ggggcgttgt tgtggagcgg
     1801 cgtcggcgac accatccgcg tctcgctctc cgccgatccg gtggaagagg tgcgcgtcgg
     1861 cttcgacatc ctgaaggcac tcgatctgcg ccaccgcggc gtgaacatca tttcctgccc
     1921 gtcctgcgcg cggcagggct tcgacgtgat cgaaaccgtc cgggttctcg aagaccgcct
     1981 caagcacatc accacgccca tgtcgctgtc gatcatcggc tgcgtggtga acgggccggg
     2041 tgaggcgcgc gagaccgatc taggcttcac cggcggcggc gcggggaaag gccacggcca
     2101 gatttatctc aacggcgagc cggcccatcg catcgacaac aaggacctga tcgagcacat
     2161 cgtcgagctg tgcgagaata aggccgccga gatcgaagcc tcgcgaaaca agctcgaagc
     2221 ggcacagtag cgatgccttc tcgttcttct tcacctcccc cttgcgggga ggcagatccg
     2281 tgcagcaaag cggaacggat cgggttggcg ggggtatagt gtcttcatga ttcccaccgc
     2341 caaactcgaa cgcctgctgg accgctttca cgcggtggag gcggagcttg cttcgggcgt
     2401 ggccggcggg acgtatgtca aactgtcgaa ggagcacgcc gaactttcgc ccatcatcgc
     2461 ggccgtggag gcctatcggg cggcggagaa gcagctcggc gaagccgatg cgctggtgaa
     2521 cgatccctcg accgattccg aaatgcgcgg cctcgccgag caggaacgcg gggagctgaa
     2581 agcgctactc gaaaggctcg aacgcgatct gaagttgaag ctgctgccga aagacgccgc
     2641 cgattcctcg tcggcgattt tggaaatccg cgccggtacc ggcggcgacg aagcggcgct
     2701 gttcgccggt gatcttctga aaatgtacac gcgctactgc cagcttcagg gctggaagac
     2761 cgatttgatg tcgctgtccg aaagcgatct cggcggcatc aaggatgcga ccctcgaagt
     2821 ggacggcacc ggcgtgttcg ccaagctgaa attcgaaagc ggcgtccatc gcgtgcagcg
     2881 cgtccccgtc accgaggcaa gcgggcgcat ccacacctcg gccgccaccg tcgccgtcct
     2941 gcccgagccc gaggacgtcg atgttgtcat caacgaaaag gatttgcgca tcgacgtcta
     3001 tcgcgcgcag ggagcgggcg gccagcacgt caacaagacc gaaagtgcgg tgcgcatcac
     3061 ccacttgccg accggcatcg ccgtagcgca gcagacggaa aagtcccaac acaagaacaa
     3121 agcccaggcg atgaagatgc tgaaggcgaa attgttcgat atggagcgcg agcgcatcga
     3181 caagcagcgc gctggcgagc gcaagagcct ggtcggttcg ggcgaccgct ccgaacgcat
     3241 ccgcacctac aatttcccgc aggggcgggt caccgaccac cgcatcaacc tgacgctata
     3301 taagctggac cggattatct ccggcgacga tctgggtgag atcatcgaag cgctggtgac
     3361 cgaggaccag gccagccgcc tggccgaact ggaggcggac ggcgcgtgag cacggagcgc
     3421 gaccaggcga agtgtgaagc ggttcgccgt ccgggcgcgc gaccctcaaa aagcaggacg
     3481 tggtgacgca ggaatcgaaa tcggcggcgc cgatccgtcc gcgccgcagc gtgctttaca
     3541 tgcccggctc caacgcgcgc gcgctcgaaa aggcgcgcac gctgccggcg gatgcgctga
     3601 tcctcgacct ggaggacgcg gtcgcccccg acgccaagga ggcggcccgc g
//
LOCUS       DASWPU010000319         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_64199,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000319 DASWPU010000000
VERSION     DASWPU010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64199"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1357
                     /locus_tag="VGC27_06825"
     CDS             170..1357
                     /locus_tag="VGC27_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282318.1"
                     /translation="MKRSAVAVVVGYTKREGLLRKSFAPLRRLKQAGVIDRILYVTWD
                     KPELDAWLADAAAMPEVELVRVPEPEVSGKPYQKKHFYQTHNIATALSLVPGDETLVV
                     KLRPDQVIDEVFLASKILAFDHCCAPSRPAACFHVAMPPSPFKMKVWTPWADSNQPFF
                     LEDGLFAALRGDAIKLVTPAGDDIVRRYGDERSLWITNVARYIAPFLPDYPIFERYLQ
                     AFNLFAQNDACRIEMLNAVITDIFFWHLVIANAWILADNFHIDCGRQGQIVFYPPEGI
                     EPGEDKGPDKPFETMAPRHPFDQVETWRAGEKPGSVFHNISRLYGRLMDDSWQHAIFI
                     NPSTTDVTRDNLHDFLRNVLLYDHGILAEAENAFYGTVEQIYRRHFPAESEAAVPARE
                     KAA"
     gene            1416..>2148
                     /locus_tag="VGC27_06830"
     CDS             1416..>2148
                     /locus_tag="VGC27_06830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY0282319.1"
                     /translation="MSHMARPAGSKKLLIACAAGIVFAYAASLAILFASHQWLLDGQG
                     RPLVVDFLAVWSAGKLVLGGAALSAYDAQLQHAGEVAVLGHEFKGALGWPYPPFFLFM
                     AAGLASLGYAWAFAVWAGATMAGYAATVGAIAKSRTAILFALAAPWTLATLFVGQNGF
                     LTAALIGLVLLNLKRLPILSGILLGLLTYKPQFGLLFPLALAMGGHWRAFVMACLTAL
                     TLIALSNAVFGPGAFAAFLHVLPQTA"
BASE COUNT          382 a          641 c          661 g          464 t
ORIGIN      
        1 cttgctcgga tatggcgcaa gcggcgtgac atgcgtttca atctgcggtt aaccggctct
       61 tcaaccacgg ttaaggtttt ttcacgatat tccgtcggcc ggagaaaagc tgcggggtcg
      121 ggcgaaagat gctgcccgtt cggctgaccg gcatcgaaag aggttgatga tgaaaaggtc
      181 tgccgttgcg gtcgttgtcg gatacaccaa aagagagggt cttctgcgca aaagtttcgc
      241 gcctttgcgg cggctgaagc aggcgggcgt catcgaccgg attctctatg tgacatggga
      301 taagcccgaa ctcgacgcat ggcttgccga cgccgcggcc atgccggaag tggagctggt
      361 gcgtgtgccg gaaccggagg tgagcggcaa gccttaccag aagaagcatt tttatcagac
      421 gcacaatatc gccaccgcac tgtccctggt gcccggcgat gaaacgctgg tcgtcaagct
      481 gcgcccggat caggtgatcg acgaggtttt cctcgcaagc aaaatcctgg cattcgatca
      541 ttgctgcgcg ccctcgcggc cggcggcgtg cttccatgtg gctatgccgc cgtcgccttt
      601 caaaatgaag gtctggacgc cctgggcgga ttccaaccag ccatttttcc ttgaggacgg
      661 cctgttcgcc gcgctgcgcg gcgacgcgat caagctggtc acgccggcag gcgacgacat
      721 cgtgcgccgt tatggcgacg agcgcagcct ctggattacc aacgtcgcgc gctacatcgc
      781 gccgttcctg ccggactacc cgatcttcga acggtatttg caagccttca acctgttcgc
      841 gcagaacgat gcctgccgta ttgagatgtt gaatgcggtg atcaccgata tcttcttctg
      901 gcatttggtg atcgccaatg cctggatcct ggccgacaat ttccatatcg actgcggccg
      961 tcagggtcag atcgtttttt atccgcccga aggaatagaa ccgggggagg ataaagggcc
     1021 ggacaaaccg ttcgagacga tggcgccgcg ccatcccttc gatcaggtcg aaacctggcg
     1081 ggccggcgag aagcccggca gcgtttttca caacatcagc cgcctctacg gccggctgat
     1141 ggacgatagc tggcagcatg cgattttcat caatccgtcg acgaccgacg tcacacgcga
     1201 taatctccac gactttctca ggaatgtctt gctctatgac cacggcatcc ttgccgaggc
     1261 ggaaaatgca ttctacggga ccgtcgagca aatataccgg cggcatttcc cggctgaatc
     1321 ggaagccgcc gttccggcac gcgaaaaggc ggcgtaaagg cggcggttca caagccgtta
     1381 acgcgcccgc aaggccgaac cgatagattg ggtgcatgtc tcatatggct cgcccggccg
     1441 gttccaagaa gctgctgatc gcttgcgctg ccggcatcgt ctttgcttac gccgcatcgc
     1501 ttgccatcct gttcgcgtct caccaatggc tgctggacgg gcagggccgt ccgctggtcg
     1561 tggattttct tgcggtgtgg tcggcgggca agctggtgct cggcggcgcg gcgctgagcg
     1621 cctacgacgc gcaactacaa catgccggcg aggttgcggt tctgggacat gaattcaagg
     1681 gagcgctcgg ctggccctat ccgccgttct tcctgtttat ggcggcggga ctggcctcac
     1741 tcggctatgc gtgggccttc gccgtctggg cgggtgcgac gatggcgggc tatgcggcca
     1801 ccgtcggagc gatcgcgaag agccgcaccg ccattctgtt tgcactggcc gccccctgga
     1861 cattggcaac cctgttcgtc gggcagaacg gtttcctcac cgcggcgctg atcgggctgg
     1921 ttctgctgaa cctgaaacgc ttgccgatat tgagcggcat tcttctcggc ctgctgacct
     1981 ataagccgca gttcggcttg ctattcccgc tggcgctggc aatgggcggg cattggcgcg
     2041 cgttcgttat ggcctgcctg accgcgctta ctctgatcgc tttatcgaat gcggtctttg
     2101 gacccggcgc cttcgccgcg ttcttgcacg ttctgccgca aacggcgc
//
LOCUS       DASWPU010000320         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_39847,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000320 DASWPU010000000
VERSION     DASWPU010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39847"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VGC27_06835"
     CDS             complement(<1..287)
                     /locus_tag="VGC27_06835"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY0282320.1"
                     /translation="MGLRDQLNNAVKEAMKARDQKRLGCLRLVQAALKDRDIAARTAD
                     SREGISDDDILGLLAKLIKSREDSIALYEQGGRPELAAAERAEIAIIREYLP"
     gene            423..1589
                     /gene="carA"
                     /locus_tag="VGC27_06840"
     CDS             423..1589
                     /gene="carA"
                     /locus_tag="VGC27_06840"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY0282321.1"
                     /translation="MTDATHSPAANPAATFSHAALVLADGSVFEGHGFGADAKAVGEV
                     CFNTAMTGYQEILSDPSYAGQIVCFTFPHIGIVGTNDEDIESLTPHVRGVIVRADARI
                     PSNYRALKTLDAWLRRHNIPGIAGIDTRALTGRIREKGMPHGAIGRGDDILALAKAAR
                     EFPGLVGLDLAKDVTSGQTSKWRQTPWAWNQGYGEQAAPTFRVVVIDYGVKRNILRML
                     AGLGADVTVVPAQATFDDVMIHKPHGVVLSNGPGDPAATGVYAIPTIKALVDAGLPVF
                     GICLGHQMLGLALGGTTKKMPQGHHGANHPVKDVETGKVEIVSMNHGFTVDSDSLPAG
                     VKQTHVSLFDGSNCGLRLEGGPVFSVQYHPEASPGPMDSHYLFARFAEAAKARV"
BASE COUNT          276 a          530 c          521 g          273 t
ORIGIN      
        1 ggcagatatt cgcggatgat ggcgatctca gcgcgctcgg ctgccgccag ttccggccgc
       61 ccgccctgtt cgtacagcgc gatggaatct tcgcggctct tgatcagctt ggcgagcagg
      121 ccgaggatgt cgtcgtcgga aatgccttcg cggctgtcgg cggtgcgcgc cgcgatgtcg
      181 cgatccttga gcgcggcctg gaccaggcgc aggcagccca ggcgcttctg atccctggcc
      241 ttcatcgcct ccttgacggc gttgttgagc tgatcgcgca agcccatctc gctctcccat
      301 gcggcaaaaa gccaatctag gccagtcggc ccgccttctc aaagcccagc gaaatcaacg
      361 caatgtcgcg ggttgaccgg cgcccgcgcg ctgcctattt tccggcaaat ccgaggcttc
      421 ccatgaccga cgccactcac tcccccgccg ccaacccggc ggcgacgttt tcgcacgccg
      481 cgctggtgct ggccgacggc agcgtattcg aaggccacgg cttcggcgcc gacgccaagg
      541 cggtgggaga agtctgcttc aacaccgcca tgaccggcta tcaggagatc ctcagcgatc
      601 cttcttatgc cgggcagatc gtctgcttca cctttcccca tatcggcatc gtcggcacca
      661 acgacgagga catcgaaagc ctgacgccgc atgtgcgcgg ggtgatcgtg cgggcggacg
      721 cgcgcatccc ctccaactat cgcgccctca agacgctcga cgcctggctg aggcgccaca
      781 acatccccgg catcgccgga atcgataccc gcgcgctgac cgggcgcatc cgcgaaaaag
      841 gcatgccgca cggcgccatc ggtcgtgggg acgacatcct cgcgctcgcc aaggcagcgc
      901 gcgagtttcc ggggctggtc ggcctcgacc tcgccaagga cgtgacctcg gggcagactt
      961 ccaaatggcg ccagacgcct tgggcgtgga accagggcta tggcgagcag gcggcgccga
     1021 cgttccgcgt cgtggtgatc gattacggcg tcaagcgcaa catcctgcgc atgctggcgg
     1081 gactgggcgc cgacgtgacc gtggtgccgg cccaggcgac cttcgacgac gtaatgatcc
     1141 acaagccgca cggcgtggtg ctgtcgaacg gtccgggcga tccggcggcg acgggcgttt
     1201 acgccatccc gaccatcaag gcgctggtgg acgcggggct gccggtgttc gggatttgcc
     1261 tcggccatca gatgctgggg ctggcgctgg gcggaaccac caagaaaatg ccgcaaggcc
     1321 atcacggcgc caatcatccg gtgaaggatg tggaaaccgg caaggtggaa atcgtttcga
     1381 tgaaccacgg cttcacggtg gacagcgatt cattgcccgc aggcgtgaaa cagacgcacg
     1441 tttcgctgtt cgacggcagc aattgcgggc tgcgcctgga aggagggccg gtgttctcgg
     1501 tgcagtacca ccccgaagcc tcgcccggcc cgatggattc gcattacctg ttcgcgcgtt
     1561 tcgccgaagc ggcgaaggcg cgggtgtagg gtttgacacg
//
LOCUS       DASWPU010000321         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_94521,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000321 DASWPU010000000
VERSION     DASWPU010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94521"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /gene="nifN"
                     /locus_tag="VGC27_06845"
     CDS             <1..1208
                     /gene="nifN"
                     /locus_tag="VGC27_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HEY0282322.1"
                     /translation="TAMDQLTTVLGADNNVVEALATICAKDKPAVIGLITTGLSESQG
                     ADIARTVAAFRAGFPQYDDVTVIPVGATDMLGCLETGFAAALEATIGAMVVETPSPLT
                     RFQRRRPRQVNVLVSAMLTPGDIDAIKSWIGAFGLTPIILPDIGDSLDGHLTDEGFST
                     LSYGGTTCAAIAAMTDSAATLVIGPSVERAADLLRQRTGIADYRFAGLMGLEECDAFS
                     QTLSELSGSAVPASIARERARYLDALVDCQFQLGGARIAVAADADLLAMIARFLVGTG
                     AEVVAAVASARADHLAGMPTDTVVVGDLEDLEWLAREAKAELIITNSHGAEIAKRLGT
                     GLLRAGFPIYDSYGAYARTWVGYGGCRQILFDIANLLAAQYREIGPYRSIYWQGTKRD
                     EEVRGGTPC"
     gene            1202..1672
                     /locus_tag="VGC27_06850"
     CDS             1202..1672
                     /locus_tag="VGC27_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEY0282323.1"
                     /translation="MLKIAFASNDRTTVNLHFGGAESLVLYDVSPGQADLIGIGTFVK
                     AEEIGETERGGLTGTVQDKVLAKLDFVKDCHAVYAASIGASSIRRLMAIGVQPIIVDS
                     GHDILDLLNEVSLGLVYGGLAWVDRAKAAAADAPAAPNPSAQVRQKLLRSADEL"
     gene            1881..2465
                     /locus_tag="VGC27_06855"
     CDS             1881..2465
                     /locus_tag="VGC27_06855"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY0282324.1"
                     /translation="MLLLIDNYDSFTYNLFHYLGQLGADVVVKRNDEISAAEALSMHP
                     EAMVLSPGPCTPNEAGICLELIAKANGTVPILGVCLGHQAIGQVYGGKVVRAPAPMHG
                     KLSRIHHTGKSVFRGLNNDFVATRYHSLTIAPESVPAALEVTAVADDGVIMGVMHKTH
                     PVHGVQFHPESIASENGHALLQNFLRMAQQPAFA"
     gene            2483..>2746
                     /locus_tag="VGC27_06860"
     CDS             2483..>2746
                     /locus_tag="VGC27_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282325.1"
                     /translation="MLKRVASGEALDAEASARVFAAIMAGGVGEIQIAAFLTALAVRK
                     PKVEEIAGAVKAMRAAMTTLEAPAGAIDLVGTGGDGHSTLNISS"
BASE COUNT          488 a          859 c          867 g          532 t
ORIGIN      
        1 ccacggcgat ggatcagttg accacggtgc tgggcgccga caacaatgtg gtcgaggcgc
       61 tggcgaccat ttgcgccaag gacaaacccg ccgtcatcgg gctgatcacc accggattgt
      121 cggaatccca aggggccgat atcgcgcgca ccgtcgcggc ttttcgcgcc ggcttccccc
      181 aatatgacga tgtgaccgtc attcccgtcg gcgccaccga catgctgggt tgcctggaaa
      241 ccggctttgc cgccgcgctg gaggcgacca tcggcgccat ggtcgtggaa acgccgtcgc
      301 cgctgacgcg cttccaacgg cggcgcccgc ggcaagtcaa tgttttggtc tccgccatgc
      361 tgacgcccgg cgatatcgac gcgatcaaaa gctggatcgg cgcctttggc cttaccccca
      421 tcatcctgcc cgatatcggc gactctttgg acggccattt gaccgacgaa gggttctcga
      481 ccctgagcta tggcggcacg acctgcgcgg ccatcgccgc catgacggat tcggcggcaa
      541 cgctggtgat cggtccgtcg gtggaacgcg ccgccgacct gttgcggcaa agaaccggca
      601 ttgccgatta tcgctttgcc ggcctgatgg ggctggagga atgcgatgcc tttagccaaa
      661 cgctgtccga gctttccggt tccgcggtgc cggcttccat cgcgcgggaa cgcgcccgct
      721 atttggatgc cttggtcgat tgccagttcc agcttggcgg cgcgcgcatc gccgtggcgg
      781 cggatgcgga cttgctggcc atgatcgccc ggtttctcgt cggcaccggc gccgaggtgg
      841 tggccgccgt cgcctccgcc cgtgccgatc atcttgccgg gatgccgacc gacacggtcg
      901 ttgtcggcga tcttgaagat ctcgaatggc tggcgcgcga agccaaggct gaattgatca
      961 tcaccaattc gcacggcgcc gaaatcgcca agcggttggg aacgggattg ttgcgcgccg
     1021 ggtttccgat ttacgattcc tatggcgcct atgcgcgaac atgggtcggt tatggcggct
     1081 gccggcagat tttgttcgat atcgccaatc tgctggccgc gcagtaccgg gaaatcggcc
     1141 cctatcgctc gatctactgg caaggcacca agcgcgacga ggaagtccgg ggcggcacgc
     1201 catgttgaag attgcttttg cctccaatga ccgcaccacg gtcaatctgc atttcggcgg
     1261 ggcggaaagt ctggtgcttt atgacgtctc gccgggacag gccgatttga tcggcatcgg
     1321 caccttcgtc aaggccgaag aaatcggcga gacagagcgc ggcggactga ccggaaccgt
     1381 acaggacaag gtgctggcca agctcgactt cgtcaaagac tgtcatgcgg tctatgccgc
     1441 atcgatcggc gcctcgtcga tccgccgcct gatggcgatt ggcgtccagc cgatcatcgt
     1501 cgacagcggc catgatattc tcgacctctt gaacgaagtc agcttggggc tggtttatgg
     1561 cggtctggcc tgggtcgacc gtgccaaagc ggccgccgcc gatgcgccag ccgcccccaa
     1621 cccaagcgcc caggttcgtc agaagctcct ccgttcggcc gatgagttgt gaccgaaggc
     1681 gggtcagcgg ctttttcgtc ggcatcttct tctgcagaaa gttgaggtgc agatttcttc
     1741 cgaatgggct aaatctcgta tgttccctcg ccttgatctg caacggcccg ttggcaaccg
     1801 gcaaaaaggg ggtgctgatg ggggtaccgt tggaccgctg taagaacttt gtgaaattat
     1861 ttcaaagaga taacgcagac atgctgctcc tgatcgataa ttacgacagc ttcacctaca
     1921 acctgttcca ctatctggga cagttggggg ccgatgtcgt cgtcaagcgc aacgacgaga
     1981 tttcggcggc cgaggccctg tccatgcatc ccgaggcgat ggttctgtcg ccggggccct
     2041 gcacccctaa cgaagcgggc atctgcctgg agctgatcgc caaagcgaac gggaccgtgc
     2101 cgatcctcgg cgtttgcctg ggccatcagg ccatcggcca agtctatggc ggtaaggtcg
     2161 tgcgtgcgcc ggcgccgatg cacggcaaac tgtcgcgcat ccatcatacg ggcaagagcg
     2221 tcttccgcgg cctcaacaac gacttcgtgg ccacccgcta tcactcgctg accatcgcgc
     2281 ccgaatccgt gcccgccgct ctggaagtga cggccgtggc cgacgatggc gtcatcatgg
     2341 gcgtgatgca caagacccat ccggtgcacg gcgtgcagtt tcatcccgaa agcatcgcct
     2401 cggaaaacgg tcacgcgctg ttgcagaatt ttctgcgcat ggcccaacag cccgcgttcg
     2461 catgagcgag gatttcgccc cattgctgaa gcgtgtcgcg agcggcgaag cgttggacgc
     2521 cgaagcgtcg gcgcgcgtct tcgccgcgat catggcgggc ggcgtcggcg aaatccagat
     2581 cgccgcgttc ctgacggcgc tggcggtgag aaagcccaag gtcgaggaaa tcgccggcgc
     2641 ggtgaaggcc atgcgcgcgg cgatgaccac gctggaagcg cccgccggcg cgatcgatct
     2701 ggtcggcacc ggcggcgacg gccactcgac gctcaacatt tcgagc
//
LOCUS       DASWPU010000322         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_88150,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000322 DASWPU010000000
VERSION     DASWPU010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88150"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VGC27_06865"
     CDS             <1..551
                     /locus_tag="VGC27_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY0282326.1"
                     /translation="AAQAIDGKAIEAAAALISETIASDRTIFACGNGGSAAISNHLLC
                     DFAKGIQTDTPHRPKVVSLSSHVELITAIGNDLSFDDVFVFQLRTAARAGDLLLTISS
                     SGNSENIVRPLQWARDNAVRTIAFTGFDGGRAAGLAEVNVHVPAHNYGVVEDLHQSAM
                     HILAQFLRMQSMTPELVGARKF"
     gene            633..1706
                     /gene="gmd"
                     /locus_tag="VGC27_06870"
     CDS             633..1706
                     /gene="gmd"
                     /locus_tag="VGC27_06870"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0282327.1"
                     /translation="MNSKRRVALITGVTGQDGAYLTELLLAKDYEVHGVKRRSSSFNT
                     ARIDKFYQDPHEPHPSLFLHYGDMTDATNLIRIVQETRPDEIYNLAAQSHVQVSFETA
                     EYTANADGLGTLRLLEAIRLLGLADKTRFYQASTSELFGKVQEIPQRETTPFYPRSPY
                     GAAKLYAYWVTVNYREAYGIHASNGILFNHESPVRGETFVTRKITRAVAAIELGLLDC
                     LYLGNIDAKRDWGHARDYVKGMWLIMQQPEPDDYVLATGESHSVREFVEKAFAETKRK
                     IVWSGKGVDEVGKDAKDGKILVRIDRNYFRPTEVDQLLGDPRKAHQKLGWKHETPFGV
                     LVAEMVAEDLKQVANEHRTNRYD"
     gene            1713..2681
                     /locus_tag="VGC27_06875"
     CDS             1713..2681
                     /locus_tag="VGC27_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEY0282328.1"
                     /translation="MNATASHRSLPYALAGKRVWVAGHSGMVGSAIVRRLASEGCEIV
                     TAELEALDLRRQSEVEAWFARNKPHAVFLAAAKVGGILANDTRRGEFLYDNLAIEANV
                     IEAARQNGVEKLLFLGSSCIYPKLAEQPMAEDALLTGSLETTNEPYAIAKIAGLKLAE
                     NYRRQYGCDFISAMPTNLYGPGDNFNLESSHVMPALIRKAHEAKVAGAVEMVVWGTGT
                     PKREFLHVDDCADALVHLMRNYSADRHVNIGTGEDISILELTELTAGIVGFKGRIVHD
                     LTKPDGTPRKLLNVAKLKGLGWRPRIPLAAGIESTYRWFLENRVRK"
     gene            2678..>3370
                     /locus_tag="VGC27_06880"
     CDS             2678..>3370
                     /locus_tag="VGC27_06880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0282329.1"
                     /translation="MKISTVTVSFNSRATIARAVQSFLEQTHPDKEMLVVDGASNDGT
                     LDIVRSFASDAIRVISEPDRGIYDAMNKGLGNFSGDAVGFLNSDDVYHDNSVLARIAQ
                     ALESADAVHGDLVMVMDHVERRAVRTWRAGPFVPGSFRRGWMPPHPTFYIRRALAQAV
                     GAFDLNYGTSADYDFMLRALELQAPRSAYIPFPLIDFMVGGASTKGVRAVVEANLGCL
                     KSRRLHLGAGLID"
BASE COUNT          663 a         1051 c         1049 g          607 t
ORIGIN      
        1 atgcggcgca ggccatcgac ggcaaggcga tcgaagcggc ggctgcactg ataagcgaga
       61 ccatcgcttc ggaccggacg atcttcgcct gcggcaacgg ggggtcggcc gcgatttcaa
      121 atcaccttct ctgcgatttc gccaagggca tccagaccga cacgccgcat cgcccaaaag
      181 tcgtcagcct gagctcccac gtggagctga ttaccgccat cggcaacgac ctctccttcg
      241 acgatgtatt cgtctttcaa ttgcgcaccg cggcgcgcgc cggggatctt ctgctgacga
      301 tcagctcctc gggaaactcc gagaatatcg tccgcccctt gcaatgggcc cgcgacaacg
      361 ccgtgcggac cattgcgttc acgggcttcg acggcggccg cgcggccggg ctggccgagg
      421 tgaatgtgca tgtgcctgcg cacaattacg gcgtggtgga ggatttgcac caatcggcga
      481 tgcatattct ggctcagttc ctccgtatgc agagcatgac gccggaattg gtcggcgcgc
      541 gaaagttcta ggccgtgaga ctttcagacg gcgatgcgat atcattgggg cgggctgcaa
      601 ttttgcttct ccggggagat acataagtgt ccatgaattc aaagcggcgc gtcgccctca
      661 ttaccggcgt gacgggccag gacggcgcct atctgaccga gctgctgctc gcgaaagact
      721 atgaagtcca cggcgtcaag cgccgctcgt cgtcgttcaa caccgcccgc atcgacaaat
      781 tttatcaaga cccgcacgaa ccccatccga gcctctttct gcattacggc gacatgaccg
      841 atgcgacgaa cctgatccgc atcgttcaag aaacccggcc ggacgagatc tacaaccttg
      901 ccgcccagtc gcacgtacag gtgagtttcg agaccgcgga atacaccgcc aacgccgacg
      961 gcctcggaac gctgcgcctg ctcgaagcga tcaggctttt ggggctggcg gacaagaccc
     1021 ggttctatca ggcgtcgacg tccgagcttt tcggcaaggt ccaggaaatc ccgcagcgcg
     1081 agacgacgcc gttctatccg cgctcgccct atggcgccgc caagctgtac gcctattggg
     1141 tcacggtcaa ttatcgcgag gcctacggca tccatgcctc gaacggcatt ctcttcaacc
     1201 atgagagccc ggtgcgcggc gaaacgttcg ttacgcgcaa gataacgcgc gccgtcgcgg
     1261 cgatcgaact ggggctgctc gactgcctct atctcggaaa catcgacgcc aagcgcgact
     1321 ggggccatgc gcgcgattac gtcaaaggca tgtggctgat catgcagcag ccggaaccgg
     1381 acgattacgt gctggcgacg ggcgaatcgc attcggtccg cgagttcgtc gaaaaggcgt
     1441 ttgccgagac caagcgaaag atcgtatgga gcggcaaggg cgtcgatgaa gtcggcaagg
     1501 acgcaaaaga cggtaagatt ctggtgcgga tcgaccgcaa ctatttccgc ccgacggagg
     1561 tggaccagct cttgggcgac ccgcgcaagg cgcatcagaa gctgggatgg aagcacgaga
     1621 cgccgttcgg cgtgctggtg gcggagatgg tcgccgagga tctcaagcaa gtggcaaacg
     1681 aacatcgcac caaccggtac gattgaaaca ccatgaacgc gaccgcatcg catcgaagcc
     1741 ttccctacgc tctcgcgggc aagcgcgtct gggtggcggg acacagcggc atggtggggt
     1801 ccgccatcgt ccgccggctc gcctcggaag gctgcgaaat cgtgacggcc gagcttgaag
     1861 cgctcgacct gcgccgtcag agcgaggtcg aggcgtggtt cgcgcggaac aagccgcacg
     1921 ccgtcttcct ggccgccgcg aaggtcggcg gcatcctggc caacgacacg cggcgcggcg
     1981 aattcctcta cgacaatctc gccatcgaag cgaacgtcat cgaggcggcg cggcaaaacg
     2041 gcgtcgaaaa gcttcttttc ctcggctcga gctgcatcta tccgaaactc gccgaacagc
     2101 ccatggcgga agacgcgctg ctcaccgggt ccctcgaaac caccaacgaa ccctatgcca
     2161 tcgccaagat cgcgggactg aagcttgccg agaattaccg gcgccaatat ggctgcgatt
     2221 tcatctcggc gatgccgacg aatctctacg gtccgggcga caatttcaat cttgaatcca
     2281 gccacgtcat gcccgcgctg atccgcaagg cccacgaggc gaaggtagcc ggcgccgtcg
     2341 agatggtggt ctgggggacc ggaacgccga aacgcgagtt ccttcatgtg gacgattgcg
     2401 ccgacgcgct cgttcatctc atgcggaact attcggcgga ccggcacgtc aatattggta
     2461 cgggggagga catctcgatc ctcgagctga cggagttgac ggccggaatc gtcggcttca
     2521 agggccgcat tgtgcacgat ttgacgaagc cggacggcac gccccgcaag ctgttgaacg
     2581 tcgccaagct gaaaggcttg ggctggcggc cgcgcatccc tctggcggcc gggatcgagt
     2641 cgacctaccg gtggttcctg gagaaccgcg tgcgtaaatg aagatcagca ccgtcaccgt
     2701 cagtttcaat tcgcgggcca ccatcgcgcg cgccgtccaa tccttcttgg aacagactca
     2761 tcccgacaag gagatgctcg tcgtcgacgg cgcgtcgaac gacggaacgc tggatatcgt
     2821 gcggtccttc gcgagcgacg cgatccgcgt catttccgag ccggatcgcg ggatatacga
     2881 cgcgatgaac aagggcctcg ggaatttttc cggcgatgcc gtggggttcc tgaactccga
     2941 tgatgtctac catgataatt cggttctggc gcgcatcgcc caggcgcttg agtccgccga
     3001 tgcggtgcat ggcgatctgg tgatggtgat ggaccatgtt gagcggcgcg ccgtgcgcac
     3061 ctggcgggcg ggacccttcg tcccgggatc gttccgccgc ggctggatgc cgccgcatcc
     3121 gaccttctac atccgccgcg ccctggccca ggccgtcggc gcgttcgacc tcaattacgg
     3181 gacttcggcg gactacgact tcatgctgag agcgcttgag ctgcaggcgc cgcgcagcgc
     3241 ctacatcccg ttcccgctga tcgatttcat ggtgggcggg gcgagcacga aaggcgtgcg
     3301 cgcggtcgtc gaggcaaatc tgggctgcct caaatcccgg cgcctgcact taggcgcagg
     3361 tctgattgac
//
LOCUS       DASWPU010000323        11847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_82372,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000323 DASWPU010000000
VERSION     DASWPU010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82372"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VGC27_06885"
     CDS             <1..752
                     /locus_tag="VGC27_06885"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY0282330.1"
                     /translation="PAQHLRASLAAIAAVAPRGIPLVVCAKGFERGSGKLPTELFRET
                     APGFEPAILSGPSFARDAAQGLPTAVTIAARLPVARRLQASFGHAAFRPYASDDLLGV
                     ALGGAAKNVYAIACGIVDGLGLGESARAALLARGFAELRRLGEALGARPETLMGLSGL
                     GDLVLTATSGQSRNYRFGREVGRGRALEELLAPGQPLAEGVATAPALVIRARNEKIEL
                     PVAETVASVLDGSLPPAAAIVRLMSRPLKPE"
     gene            758..1174
                     /locus_tag="VGC27_06890"
     CDS             758..1174
                     /locus_tag="VGC27_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY0282331.1"
                     /translation="MAYWLFKSEPETWSWQQQKKKGARGEPWTGVRNYQAANAMKAMK
                     KGDLGFFYHSGEERRIVGIVEVIGEYRPDPTDASGRFGMVAVKAVRDMPRPVSLAEIK
                     FAPELGGMVLVRNSRLSVQPVNPGEWRTVCAMGGIE"
     gene            complement(1297..1896)
                     /locus_tag="VGC27_06895"
     CDS             complement(1297..1896)
                     /locus_tag="VGC27_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282332.1"
                     /translation="MRELIARLLAASSELLAAVQASVKTHKGWWAGGGGAAAVAIAAV
                     VILFGGFLGPSGKDICTVALEHAREYGVLPGSATLASTTAKSTDERNRRLCTAKVGDD
                     IYNITADVIRTDTADLKCKSKDVTKNKNCIALYSVAREDGLTTYQVRQVPDDDADVPA
                     VAVDAGGEAQPALDDSDIQPTTGTRSENNGQQQGAAPPQ"
     gene            complement(2073..3545)
                     /locus_tag="VGC27_06900"
     CDS             complement(2073..3545)
                     /locus_tag="VGC27_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY0282333.1"
                     /translation="MPATLTTVSYIGAIILFILSLGGLSNPETSRRGNWYGIVGMTIA
                     VLATVFGARLVEVPGGHEEWKLVMPDGLVWIVVAMAVGGTIGIIAARMVKMTQMPELV
                     AIMHSLVGLAACTIGFASFIDTSTTFPTAAEHSIHLMEIYIGVLIGAVTFSGSVIAFG
                     KLSGKISGKPVILPARHLINLAGLLVVIYYGYVFMVGGGLHGGTPTFNEMLPLIVMTV
                     ISLLFGIHMVMAIGGADMPVVVSMLNSYSGWAAAATGFMLGNDLLIVVGALVGSSGAI
                     LSYIMCRAMNRHFLAVIACGFGTAEGSAAKKSGDAGPVGEVTPISSAETAELLRESKN
                     VIIVPGYGMAVAQAQHTVYEITKWLREKGVNVRFGIHPVAGRMPGHMNVLLAEAKVPY
                     DIVLEMDELNEDFPDTDVAMVIGANDIVNPAAEDDPTSPIAGMPVLQVWKAKTSIVMK
                     RSMASGYAGVDNPLFYKENNRMLFGDAKKMLDEVLTALKA"
     gene            complement(3558..5123)
                     /locus_tag="VGC27_06905"
     CDS             complement(3558..5123)
                     /locus_tag="VGC27_06905"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY0282334.1"
                     /translation="MREIMPQTIGIPKETFAGEKRVATAPEVVSKLIKLGFAVIVESG
                     AGDGASFYDDAFAAAGAKIANSAAEVWSSSDIVFKVRAPDPGEVALLKEGTTLISFIW
                     PAQNPDLMKALAARKVTVLAMDAVPRLLSRAQKMDALTSMAGISGYRAVIEAANAFGR
                     YFNGQITAAGKIPPAKVFVAGLAAIGTSVGLGAIVRANDTRAEVADQVKSMGAEFIKV
                     DYEEEGSGGGGYAKVMSEGFQKAQREMYAKQAKDVDIIITTALIPGKPAPRLITAEMV
                     ASMKPGSVIVDMAAEQGGNCELTEPGKAVVKHGVTIIGYTDLASRLAKQASTLYATNQ
                     LRLTEELCKAKDGQIVVNMEDDAIRGFTVIKDGEITWPAPPLKLPAPPAPKAAAAPVP
                     VKEKSAFAKAVAAFTLPVLALALFALIGLFAPPAFIGHFTVFVLAVFVGYMVVWNVTP
                     ALHTPLMSVTNAISSIIAIGALIQVAPHVPDNVSRLNDVIRWIAIVGIALTSINMFGG
                     FAVTRRMLMMFRK"
     gene            complement(5474..6457)
                     /locus_tag="VGC27_06910"
     CDS             complement(5474..6457)
                     /locus_tag="VGC27_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0282335.1"
                     /translation="MSGAANLPPVSGAGKVDFPDLETPLAELAGAPPPAPAWFAETVA
                     IPPESRFVEVDGAAIHYLRWGDKTRPGLLLVHGNAAHAFWWSFIAPFLAREYNVAAMD
                     LSGMGDSDWRPGGYTMEQFAREELAVCEDAGMFVPSEPPIVVAHSFGGFVTILTGALY
                     GERLAGTVIVDSPVNPPGRPGIAAHRQSAHKIYPSMAAALGRFRLLPPQTCDNLYLLD
                     WVARHSLKETGGGFSWKFDPAIWRDFSIGDIAARLKATKCRIAIFRGEHSILLPPQIG
                     QYMFNLLGRAAPVVEIPQAQHHIMLDQPLAFVAALRALLADWEHSVSRRRL"
     gene            6942..7964
                     /locus_tag="VGC27_06915"
     CDS             6942..7964
                     /locus_tag="VGC27_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282336.1"
                     /translation="MSIHLMKIMLLAGACMALTGPANAQINWSGFGELAGGGIPDSSP
                     HSTTPLVLAGGAHVNAAFESGLNLQFDAEGTRDFFNIKDPAGRYPRDNFSNSDKMTKY
                     GLGVHLDLRGDQYLVGGLASIGNSDWTRYNNRLVTVGVEGALFLDRTTLFSQLTYSRA
                     VQGHFAGDGLDSPYLYTGARYFLRDNLMLEADAGAGTIETNDMMYTGTNVGPVDTTTF
                     IIDTNYNGNALHWGTKVEYRFQDSPVSLAFDYRGSYSTWSYWHIVELRSSGGGRFNCN
                     ASFTVHRTENLFMLRLRYYFGQDSLIANDRNGASISDYNPWYGTEPVSEGFAGNLSAL
                     FPPPSC"
     gene            8132..9643
                     /locus_tag="VGC27_06920"
     CDS             8132..9643
                     /locus_tag="VGC27_06920"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0282337.1"
                     /translation="MAQTILIVDDDPVQRRLLDAAISRAGMTVITAPGGGPALDLVTG
                     PRGDQISLMLLDLVMPDMDGLEVLAKLRVTHPDLPVIVLTAKGGIDSAVEAMRAGAND
                     FLVKPASPERITVSIRNQLKIGTLSGEVSRLKKKTENRLAFDDLVAKSEEMKQVFRLG
                     QRAAQSTIPILIEGESGVGKELIARAIQGSSDRAGKPFVTVNCGAIPENLIESILFGH
                     ERGSFTGASEKHLGKFQEADGGTLFLDEIGELRLDMQVKLLRALQEGEVDPVGSKRPV
                     KVDVRIMSATNRDLSELTRDGRFREDLFYRLNVFPIFVPPLRDRHGDIPALARHFISR
                     FATEEHKPVAGLSPEASDLLERFSWPGNVRQLENTIFRAVVLCDSSLLDICDFPQIAA
                     AMGVEAKSQRAFAAPNSYNADMFTPATTLSAASPYALSATDAAGHMRKLEEIESEIIR
                     LAISRYDGHMSEVARRLGIGRSTLYRKLKEFGLEPDEGEAPDSAGDPPRQAAG"
     gene            complement(10005..10250)
                     /locus_tag="VGC27_06925"
     CDS             complement(10005..10250)
                     /locus_tag="VGC27_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEY0282338.1"
                     /translation="MAKSGFAKEHLRSFIERIERLEEERAALGADIREVYAEAKGQGF
                     DSKIMRQVVRLRKLDKADFQEHEAMLDLYLGALGMRS"
     gene            10506..10670
                     /locus_tag="VGC27_06930"
     CDS             10506..10670
                     /locus_tag="VGC27_06930"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0282339.1"
                     /translation="MNNLLTRFVRDDSGATAIEYGLIAALIAVVIIGVLTTIGTGLSA
                     KFNSIATKLQ"
     gene            complement(10720..10866)
                     /locus_tag="VGC27_06935"
                     /pseudo
     CDS             complement(10720..10866)
                     /locus_tag="VGC27_06935"
                     /inference="COORDINATES: protein motif:HMM:NF018545.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
     gene            11112..11507
                     /locus_tag="VGC27_06940"
     CDS             11112..11507
                     /locus_tag="VGC27_06940"
                     /inference="COORDINATES: protein motif:HMM:NF018037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HEY0282340.1"
                     /translation="MRFIFAVLTAILLATGPAQAGFKVCNKANLPAKVALGRFDGKVW
                     RSEGWWTILPQKCETLLSGPLDARYYYLYGTDGGSGTWNGDKGFCTEAVGPFSIKGRG
                     NCAARHYDRKGFFEVDTGDDLDWTQSLSD"
     gene            11634..>11847
                     /locus_tag="VGC27_06945"
     CDS             11634..>11847
                     /locus_tag="VGC27_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY0282341.1"
                     /translation="MRRRRKTKILATLGPSSATPEGIRALFEAGADAFRINMSHTSHA
                     GLAEFHHTVRALEESVGRPIGILVDMQ"
BASE COUNT         2192 a         3747 c         3672 g         2236 t
ORIGIN      
        1 ccccggccca gcatttgcgc gccagcctgg ccgccatcgc ggcggttgcg cctagaggca
       61 ttccgctggt ggtctgcgcc aagggttttg aacggggcag cggcaagctg ccgaccgagc
      121 tgttccgcga gaccgccccc ggcttcgagc cggcgatcct gtcggggccg tctttcgcgc
      181 gcgacgcggc gcagggattg cctacggccg tgaccatcgc cgcccggctg cccgtcgccc
      241 ggcgcctgca agcgagtttc ggccacgccg ccttccggcc ctatgccagc gacgatcttc
      301 tcggcgtggc gttgggcggg gcggcgaaga acgtctatgc catcgcctgc ggcatcgttg
      361 acggcctggg tctgggcgaa agcgcgcgcg cggccttgct ggcgcgcggc ttcgccgaac
      421 tgaggcggct gggagaagcg ctgggcgcgc gcccggaaac gctgatgggc ctgtcggggc
      481 tgggcgatct cgtgctcacc gccaccagcg ggcaatcgcg caattaccgc ttcggccgcg
      541 aggtcggaag gggccgcgcg ctggaggagc ttctggcgcc gggacagccg ctggccgaag
      601 gcgtcgcgac ggcgccggcg cttgtcatcc gcgcccgcaa cgagaagatc gaattgccgg
      661 tggccgaaac cgtcgccagc gtgctcgacg gctctctgcc acccgccgcg gccatcgtgc
      721 ggctgatgag ccggccgctg aaaccggaat agcaacgatg gcgtattggc tgttcaagag
      781 cgagccggag acctggtcct ggcagcagca gaagaagaag ggcgcgcgcg gcgagccctg
      841 gaccggcgtg cgcaactatc aggccgccaa cgccatgaaa gcgatgaaga aaggcgatct
      901 cggcttcttc tatcactcgg gggaagaacg gcggatcgtg gggatcgtcg aggtcatcgg
      961 cgaatatcgc cccgacccta ccgacgcaag cggccgtttc ggcatggtcg cggtcaaggc
     1021 ggtgcgcgac atgccacggc ccgtgtcgct ggccgagatc aagttcgcgc ccgaactcgg
     1081 cggcatggtg ctggtgcgca attcgcggct ttcggttcag ccggtgaacc cgggcgaatg
     1141 gcgaaccgtc tgcgccatgg gcgggatcga gtaggccttg attttcctcc cctttcagcc
     1201 gccctcgctg aagcttcggc gggcctggcg agtcacattc gcccggcgaa gctttggcat
     1261 agccgggggg agggaacttt gctcgtaatt cggcggctat tgcggcgggg cggcgccctg
     1321 ctgctggccg ttgttttcgc tgcgcgtgcc cgtggtcggc tgaatatcgc tgtcgtctag
     1381 cgcgggctgt gcctcgccgc cggcgtcaac cgcaaccgcc ggcacatcgg catcgtcgtc
     1441 gggcacctgc cgcacctgat aggtcgtcaa accgtcctcg cgggcgacgc tgtagagtgc
     1501 gatgcagttc ttgttcttcg taacgtcctt gcttttgcat ttgaggtcgg cggtgtcggt
     1561 acggataacg tcggcggtga tgttgtagat gtcgtcgccg accttggccg tgcacagcct
     1621 gcggttcctc tcgtccgtgc tcttcgcggt cgtgctcgcc agggtggcgc tgccgggaag
     1681 gacgccgtat tcgcgggcgt gttcgagcgc caccgtgcag atgtccttgc cgctcggccc
     1741 gagaaagccg ccgaacagga tcaccacggc cgcaatcgcc acggctgccg ctccgccgcc
     1801 gccggcccac caccctttgt gcgtcttgac ggatgcctgg accgccgcga gcagctccga
     1861 actcgccgcc agcaatcgtg caatcaattc gcgcaccggg tgttcctttc gttctgttcc
     1921 gccaacaagg gggccataca tatcccgcac ggccgcacgc ttccagcccc cggaggcgct
     1981 gccggctaaa tcgtgcttaa tgttgcgggg aagagctcga aacgaaagcc ccgccgactt
     2041 tacgccggcg gggcttgcga ttcagaagcc gctcaagcct tgagcgcggt tagcacttcg
     2101 tcgagcatct tcttggcgtc gccgaacagc atcctgttgt tctccttgta gaacagcgga
     2161 ttgtcgacgc cggcatagcc cgacgccatc gagcgcttca tcacgatcga ggtcttggcc
     2221 ttccacacct gcagcaccgg catgccggcg atcgggctgg tgggatcgtc ctcggccgcc
     2281 ggattgacga tgtcgttggc gccgatgacc atcgccacgt cggtgtccgg gaagtcctcg
     2341 ttgagttcgt ccatctccag cacgatgtcg taaggcacct tggcttcggc cagcagaacg
     2401 ttcatgtgcc ccggcatgcg cccggcgacc ggatggatgc cgaagcgcac attgacgccc
     2461 ttttcgcgca gccacttggt gatctcgtac accgtgtgct gcgcctgggc gaccgccatg
     2521 ccgtagcccg gcacgatgat gacgttcttg gattcgcgca gcagttcggc ggtctcggcc
     2581 gagctgatcg gcgtcacttc gcccaccggg cccgcatcgc ccgacttctt ggcggcggat
     2641 ccttcggccg tgccgaagcc gcaggcgatg acggcgagga agtgacggtt catcgcccgg
     2701 cacatgatgt aagagaggat cgcgcccgac gaaccgacca gcgcgccgac gacgatcaac
     2761 agatcgttcc ccagcatgaa gcccgtggcc gccgccgccc accccgaata ggagttgagc
     2821 atcgacacca ccaccggcat gtcggcgccg ccgatcgcca tcaccatatg gatgccgaag
     2881 agcagcgaga tgaccgtcat cacgatcaac ggcaacatct cgttgaacgt cggcgtcccg
     2941 ccgtggaggc cgccgccgac catgaagacg tagccgtagt agatcaccac cagaaggccg
     3001 gccaggttga tgagatggcg ggcgggaagg atcaccggct tgccgctgat tttacccgac
     3061 agcttgccga aggcgatcac cgaaccggag aaggtcaccg cgccgatcag gacgccgatg
     3121 tagatctcca tcagatggat cgaatgttcg gctgcggtcg gaaaggtggt cgaagtatcg
     3181 atgaagctgg caaagccgat ggtgcaggcc gccagaccga ccaggctgtg catgatggcg
     3241 accagttcgg gcatctgggt catcttgacc atgcgggccg cgatgatgcc gatggtgccg
     3301 ccgaccgcca tggccacgac gatccagacc agaccgtcgg gcatgaccaa cttccattcc
     3361 tcgtgcccgc cggggacttc gaccagacgg gcgccgaaca cggtcgccag cacggcgatc
     3421 gtcatgccga cgatgccgta ccagttgccg cggcgcgagg tttccggatt ggaaaggccg
     3481 ccgaggctga ggatgaagag aatgatggct ccgatgtagg agactgttgt cagagttgca
     3541 ggcatgtgtg cggtccccta tttccggaac atcatcagca tgcgccgcgt caccgcgaag
     3601 ccgccgaaca tgttgatgga cgtgagcgca attccgacga tggcgatcca gcggatcaca
     3661 tcgttcaacc gcgaaacgtt gtcgggaacg tgcggcgcga cctggatgag ggcgccgatg
     3721 gcgatgatgg aggaaatcgc gttggtgacg ctcatcagcg gggtatgcag agccggcgtg
     3781 acgttccaca ccaccatgta gccgacgaaa acggccagca cgaacaccgt gaagtgtccg
     3841 atgaaggccg gcggagcgaa aagcccgatc agcgcgaaca gcgccagggc gaggacgggt
     3901 agcgtgaaag cggcgaccgc cttcgcaaac gcgctcttct ccttgaccgg cacaggggcc
     3961 gcagcggcct tgggcgcggg cggcgcggga agtttgagcg gcggggcggg ccaggtgatc
     4021 tcgccatcct tgatcacggt gaagccgcgg atcgcatcgt cctccatgtt gacgacgatc
     4081 tgaccgtcct tggccttgca tagctcttcg gtgaggcgca gctggttcgt ggcgtagagc
     4141 gtcgaagcct gcttggcgag gcgcgaggcc agatcggtat agccgatgat ggtcacgccg
     4201 tgcttgacga cggccttgcc cggctcggtc aattcgcagt tgccaccctg ttcggccgcc
     4261 atgtccacga tcacgctgcc aggcttcatc gaggcgacca tttcggccgt gatgagacgg
     4321 ggcgcgggct tgccgggaat gagggccgtc gtgatgatga tgtcgacgtc cttggcctgc
     4381 ttggcgtaca tctcgcgctg ggccttctgg aagccttcgc tcatcacctt ggcatagccg
     4441 ccgccgcccg agccttcttc ctcgtagtcg accttgatga actcggcgcc catcgacttg
     4501 acctgatcgg cgacttccgc gcgcgtatcg ttggcgcgaa cgatggcgcc gaggccgacg
     4561 gaggttccga tcgccgcaag accggcgacg aacaccttgg cgggcgggat cttgcccgcc
     4621 gcggtgatct ggccattgaa gtagcggcca aaagcgttcg ccgcttcgat gaccgcgcga
     4681 taaccgctga tgcccgccat cgaggtcagc gcgtccattt tttgcgcgcg cgacaagaga
     4741 cgcggtacgg cgtccatggc cagcacggtc accttgcggg cggccagcgc cttcatcaaa
     4801 tccgggttct gcgccggcca gatgaagctg atcagcgtgg tgccttcctt cagcagggcg
     4861 acttcgcccg ggtcgggcgc gcgcaccttg aagacgatat cgctcgacga ccagacctcg
     4921 gccgcgctgt tggcgatttt cgcgccggcg gcggcaaagg cgtcgtcgta aaagctcgcg
     4981 ccgtcgccgg cgccgctttc gacgatcacc gcaaagccga gcttgatcag cttggaaacg
     5041 acttccggcg ccgttgctac gcgcttctcg ccggcaaagg tctctttcgg tattccaatg
     5101 gtctgaggca tgatttcacg catctggttg gagtgattga gaacggcgga caagcctcca
     5161 ttcgggcgat gggtaagatg tcggcagaga tcagccgcgc aaaccgggaa ggacgggggt
     5221 cggcatgatc aaagaggcgt cggcgattgt cgtaccggat tgcccgccgt tgccgccgtg
     5281 cgcagccaag cccgcccttg aattacgcgg cgggaatata gcaaagatgc cgcataagca
     5341 acgtttgccg gtgcggcatt gaataccatt cccgaagtct gaaatggccg tgtaattccg
     5401 cgcattccgc cggtttgcat tgcggtcttt gcttggccgc gcgcacgaac tgttgcagat
     5461 tctttcagca gttctacagc ctgcgccgcg acaccgaatg ttcccagtcc gccagcagcg
     5521 cgcgcagcgc cgccacgaag gccaggggct ggtccagcat gatgtgatgc tgggcctggg
     5581 ggatttcgac caccggtgcg gcgcgcccca ataggttgaa catgtactgc ccgatctggg
     5641 gcggcagcag gatcgaatgc tcgccgcgga agatggcgat tcggcatttg gtggccttca
     5701 gccgcgcggc gatgtcgccg atggagaaat cgcgccagat cgccggatcg aacttccagg
     5761 agaagccgcc gccggtctct ttcagcgaat ggcgcgccac ccaatccagc aaatagaggt
     5821 tgtcgcaggt ctgcggcggc agcagccgga agcggccgag ggccgcggcc atgctgggat
     5881 agatcttgtg tgcactttgg cgatgggcgg cgatccccgg ccggcccggc ggattgaccg
     5941 gcgaatcgac aatcaccgta ccggccagtc tttcgccgta cagcgcgccg gtcagaatgg
     6001 tgacgaagcc gccgaaactg tgcgccacga cgatcggcgg ttcggacggc acgaacatgc
     6061 cggcgtcttc gcacaccgcc agttcctcgc gcgcgaactg ttccatcgtg taaccgccgg
     6121 gccgccaatc gctgtcgccc atgccggaca gatccatcgc cgcgacgttg tactcgcgcg
     6181 ccaggaacgg cgcgatgaag ctccaccaaa aagcgtgcgc ggcattgccg tggaccagca
     6241 gcagccccgg ccgcgtcttg tcgccccaac gcagatagtg aatggccgcg ccgtccactt
     6301 cgacgaagcg gctttccggc gggatcgcca ccgtctccgc gaaccaggcg ggcgcgggcg
     6361 gcggcgcgcc ggcgagttcg gcgagcggag tctccagatc gggaaagtcc actttccccg
     6421 ccccggagac gggaggaaga ttcgctgcgc cgctcatggc cgcgaaggct ggcacgcttg
     6481 cgccatccgc tcaaggcggg gccggggctg tgcggcgggc cgtgtccagg cgttccggca
     6541 acccaatcac cgtctttcca attgcacgtg cgtcacagaa atcttcgctg ccaatagtca
     6601 ttcggcggct ccgcaaagtg gagaaacacg atctctgctc gagtatttct ccagcaacga
     6661 gacctcatgg tgcaggagcc aagctgcttc ttccgctcaa agccaagcaa aatttaggct
     6721 gccgtcatac gaagcagtat tcggaagtag ttaagtatgc gtctcattca acttgctatc
     6781 atactctaag aacttgcaga aaaagataca tcacgtatca agcagaaatt tgataatatt
     6841 gctcggttat ttgcctttgc tctatgaatt cttggccatt gttcaaagag atggcggaag
     6901 tcggtttctt acggcgatgg ctacaactag tggggggcgt gatgtcgata catctgatga
     6961 aaatcatgct gctcgccgga gcatgcatgg cactgactgg tccggcaaac gcacaaatca
     7021 actggtcagg ctttggcgaa ctcgccggcg gcggaatccc ggacagttct ccacattcca
     7081 ctaccccgtt ggttctggcc ggaggcgccc atgtcaacgc ggcgttcgag tccggactga
     7141 accttcagtt cgatgccgaa ggcacgaggg acttcttcaa catcaaggac cctgccggca
     7201 ggtacccgcg cgataacttt tcgaacagcg acaaaatgac gaaatacggg ctgggtgttc
     7261 acctcgatct acgcggcgac caatacctcg taggcggcct cgcttccatc ggtaactcgg
     7321 actggacacg ctataataac cggttggtta cagtcggcgt ggagggcgcg ttgttcctcg
     7381 accgcacaac tctcttctcg cagctgacct attctcgcgc ggtgcaggga cacttcgccg
     7441 gggacggatt ggactcgcca tacctgtaca cgggcgcccg ctatttcctt cgcgacaatc
     7501 tgatgttgga agccgacgcc ggcgcgggca cgatcgaaac caacgatatg atgtacacgg
     7561 gaaccaacgt cggccccgtc gacaccacga ccttcatcat cgataccaac tacaacggca
     7621 acgcgctgca ctggggcacg aaggtcgaat acaggttcca ggattcgccg gtgagtttgg
     7681 ccttcgatta ccgaggctcc tattcgacct ggagctattg gcacatagtc gagctgcgct
     7741 ccagcggcgg cggccgtttc aactgcaatg ccagttttac tgtgcatcgc accgagaacc
     7801 tgttcatgct gagactgcgc tattacttcg gacaggattc gctgatcgca aacgaccgca
     7861 acggcgcgag cataagcgac tataacccct ggtacggcac ggagcccgtc tcggaaggat
     7921 ttgcaggcaa tctctcggcc ctctttccac cgccgagctg ttgaaaggtc tctgttcagc
     7981 cgcgtgagcg cgatccgccg atcgcgttcg cgcgttttta cggtttgttc accatgctgc
     8041 cctgtaccga gattgtgcca cacaaggaca tcccttaaac agacctttac cagtcgcggg
     8101 ccaccatcgc gctctcacag ccgagggccc gatggcacag acgattctca ttgtcgacga
     8161 cgatcccgtg cagcgccgcc tgcttgatgc cgcgatcagc cgcgccggta tgaccgtcat
     8221 caccgcgccc ggcggcggcc cggcgctcga tctggtgacc ggtccgcgcg gcgatcagat
     8281 ttcgctgatg ctgctcgatc tcgtgatgcc cgacatggac ggactggaag tgctcgccaa
     8341 gctgcgcgtc acccatcccg acctgccggt gatcgtgctc accgccaagg gcggcatcga
     8401 ttccgcggtg gaggcgatgc gcgcgggcgc caacgatttc ctggtcaagc cggccagccc
     8461 cgaacgcatc acggtctcga tccgcaacca gctcaagatc ggcaccttgt cgggcgaagt
     8521 ctcccgcctg aagaagaaga cggagaaccg tctcgccttc gacgacctcg tcgccaaatc
     8581 ggaggagatg aaacaggtct tccggctcgg acagcgcgcc gcgcaatcga ccatcccgat
     8641 tttgatcgaa ggcgaatccg gcgtcggcaa ggagttgatc gcgcgcgcca tccagggttc
     8701 ctcggaccgc gcgggaaagc ccttcgtgac cgtcaattgc ggcgccattc ccgagaacct
     8761 catcgaatcg atcctgttcg gccacgaaag agggtctttc accggcgcca gcgaaaagca
     8821 tctgggcaag ttccaggaag ccgacggcgg caccctgttc ctcgacgaaa tcggcgagct
     8881 gcgcctcgac atgcaggtca agctgctgcg cgcgttgcag gagggcgaag tcgatccggt
     8941 cggctccaag cggccggtaa aggtcgatgt gcgcatcatg tcggcgacca accgcgacct
     9001 ttcggaactg acgcgcgacg gccgcttccg cgaagacctg ttctaccgcc tcaacgtgtt
     9061 cccgatcttc gtgccgccgc tgcgcgaccg ccacggcgac atcccggcgc tggcgcgcca
     9121 tttcatctcc cgcttcgcca ccgaagagca caagccggtc gccggattgt cgccggaagc
     9181 gagcgacctg ctcgaacgct tttcctggcc cggcaatgtg cgccagctcg aaaacacgat
     9241 cttccgcgcg gtggtgctgt gcgacagctc gctgctcgac atctgcgact ttccgcagat
     9301 cgcggccgcc atgggcgtgg aagccaaatc gcagcgggcc ttcgcggcac cgaactcgta
     9361 caacgccgac atgttcacgc cggccaccac cctgtccgcc gcttcgcctt acgcgctctc
     9421 ggccaccgat gccgcgggcc atatgcgcaa gctggaagag atagagtccg agatcatccg
     9481 cctcgccatc tcgcgctatg acgggcacat gtccgaagtc gcgcgccggc tgggcatcgg
     9541 ccgctcgacg ctgtaccgca agctgaagga attcggcctg gagccggacg aaggcgaagc
     9601 gccggactcc gccggcgatc ccccgcgcca agccgcggga tagagcctgt cgggatcgtg
     9661 caaattcacc tattccctcg cttgtcatgg cccacaacag tgggccatcc agagggagag
     9721 ggacgcaaga cccgcagaaa aatctctttt acaacatcga aaccgtagca gtatcgcctg
     9781 gatggcctgc ttatgcaggc catgacacgg caagagacgg acaaactcta ttcgaagcgg
     9841 ctctagtgtc ccgattccga aattcgcacc atttctatat cggcgtcggg cgaatttcgg
     9901 aatcgaaagg acactagcaa cctattgaat cctagtgtcg ttttggtttc gaagttcgct
     9961 tgcggcccga tataacgaat ggtgcgaact tcgaaaccac gacactagct gcgcatcccc
    10021 agcgcgccga gatacagatc cagcatcgct tcgtgttcct ggaagtcggc cttgtcgagc
    10081 ttcctgaggc ggacgacctg acgcatgatc ttgctgtcga agccctggcc tttggcttcg
    10141 gcgtaaacct cgcggatatc ggcgccgagc gccgcgcgct cttcctccaa ccgctcgata
    10201 cgctcgatga acgagcgcaa atgctctttg gcaaatccgg atttggccat tgtcaggact
    10261 ccatcacgcg gttgcggccc acggcgtgtg ccgttgtctc cgaaaaacac tgaatgcggc
    10321 tttgtcttgc attttaatca aatacatcca tcggattttt tattcattcg tcttgctgga
    10381 cgcgaacttg aattcttaaa ggcggattaa ggtctgacgc ctatcttcgt gcggtcacgg
    10441 ccatgaatcg caacaagcgg cgcgcgccgg ggcgttttgg gtgtccaata gtggaggtct
    10501 ctctaatgaa caatcttctc actcgctttg ttcgcgacga ttcgggcgcg acggccatcg
    10561 aatacggtct gatcgcggcc ctcatcgccg tcgtcatcat cggcgtgctg acgaccatcg
    10621 gcacgggcct cagcgcgaag ttcaactcga tcgcgacgaa attgcaatga cgccgtcgta
    10681 taggagagcg gcgttacagg tgcaacgcca cgtcttctgc tagctgtggg tcttttgcgt
    10741 ggccgcgaat gaagctgact gttccggcgt cacttccttt cggtagtgtg ccttccactc
    10801 ggaaggtgcc atgccgtagg taatttcgcg cgcttcgccc ttgcccatgg cgatgccttg
    10861 tccggccgcc gcctcgcgct acaactcgcc gaggcctttg cggcaatcct gatctcaaga
    10921 cgatatcctg cccgccgctg cgtgcaggcg cgtccatcat ggtctccatg gctgtgacgc
    10981 cgccctcggt ccgggcccgc cggaccggcc ggaaacccgc ctcgccagag gctgcgtcac
    11041 agatcagtcg gcaagccgat ctaaacaggc gttgacagat tcggcccctt gggcgacacc
    11101 gttcggaccc catgcgcttc atatttgccg tcctgaccgc catcctgctt gcgactggtc
    11161 ccgcccaggc gggcttcaaa gtctgcaaca aggcgaatct gcccgcaaaa gtggccctcg
    11221 gccgcttcga cgggaaagtc tggcgcagcg aaggctggtg gacaattctt ccgcaaaaat
    11281 gcgagaccct gctctccggc ccgctcgacg cacgctatta ttacctttat ggcaccgatg
    11341 gcggttcggg gacgtggaac ggcgacaagg ggttctgtac ggaagcggtc ggaccgtttt
    11401 cgatcaaggg ccgcggcaac tgcgcggcgc ggcattacga ccgaaaaggg ttttttgagg
    11461 tcgatacggg cgacgatttg gactggaccc aatcgctttc ggactagagc atttcaccgt
    11521 ttcacggaac cgatgaaatg ctctagattc ttagtttgtc gcgttttcgg acggaaaacc
    11581 ggtttccact tttcctgaaa acgctttaga gcggatacag cggactgaac tagatgcggc
    11641 gcaggcgtaa gacaaaaatc ctggcaaccc tcggtccgtc cagcgcgacg ccggaaggca
    11701 tccgtgcgct gttcgaggcg ggagccgacg ccttccgcat caatatgagc cacacttccc
    11761 acgccgggct ggccgagttt caccatacgg tgcgcgccct ggaagagagc gtcggccggc
    11821 cgatcggcat cctggtcgac atgcaag
//
LOCUS       DASWPU010000324         8454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_106883,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000324 DASWPU010000000
VERSION     DASWPU010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106883"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VGC27_06950"
     CDS             complement(<1..359)
                     /locus_tag="VGC27_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEY0282342.1"
                     /translation="MSQIRLPFDAVLIAGPTASGKSAAALALAEAVDGVVVNADSMQV
                     YREVRILSARPSAADRTRAPHLLYGHVGASERYSVGRYQTDALAALAEARALGRLPIF
                     VGGTGLYFAALTEGLAD"
     gene            351..1244
                     /gene="serB"
                     /locus_tag="VGC27_06955"
     CDS             351..1244
                     /gene="serB"
                     /locus_tag="VGC27_06955"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEY0282343.1"
                     /translation="MAQEFSHVLNVISKNAGPLAVDIGRWLGPWLGQSPLSWLSPDYA
                     FDAPFTGDAKKALTEARAQTKGLAADINVVATAGRRKRLLIADMDSTIINCECLDELA
                     GIAGLRKKVAGITERAMRGEIEFTAALRERLGLLKGLPLEALERVWHDRVRLNPGAKE
                     LTATMKANGARTLLVSGGFTFFTGRVAEAAGFDSHQSNVLYDDGKALTGEVREPIMGR
                     TAKLQALERALQQYGLKPQDVLAVGDGANDLEMIQRAGLGVAYHAKPVVAAAASASIA
                     HGDLKALLYLQGYREEEIVSS"
     gene            complement(1321..2703)
                     /locus_tag="VGC27_06960"
     CDS             complement(1321..2703)
                     /locus_tag="VGC27_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY0282344.1"
                     /translation="MASLAFAAMPAGARGAPDSFADLSAKLLPTVVNIATTQTLKANQ
                     KSPLPDLPPGSPLQDLFKNFLGPGRGAPRHVTSLGTGFIIDQSGLIVTNNHVVENAEQ
                     IAVTLNDGTTLPAKLIGRDEKTDLALLKVSARKPLPVARFGDSDKTRIGEWVIAIGNP
                     FGLGSTVTAGIVSARNRDIEAGPYDDFIQTDAPINKGNSGGPLFDMSGNVVGVNSAIF
                     SPTGGSVGIAFAIPSNMARQVIGQIRQFGSVRRGWVGIRIQPVTRDIAEGMRLPAAGG
                     ALVADVTPNGPAAKAGIRNGDVITAFDGKRIADPRSLSRLAADTQIGKTVNVDLLRKG
                     KKITVRLTVVRLSEGGAKPQPPTPAKKKKLSQLGLSLSPVDSGARAEFKLPGGVQGIV
                     VTSVIPDSQAAEQNLQAGDVIVSMQDRPVRTPADFQRQLESDVKAGRKVEILLVNRGG
                     ALAFVALRLR"
     gene            complement(3300..3680)
                     /locus_tag="VGC27_06965"
     CDS             complement(3300..3680)
                     /locus_tag="VGC27_06965"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEY0282345.1"
                     /translation="MRDSERRTTKRAKKLRRHMTDAEVILWSRLRGRAAEGFRFRRQH
                     PIGPYIADFACAMTRLIVEVDGGTHSTDAELDHDRHRDAFLKSRGWRLFRVTNEDVYK
                     RLDSVLEGICLHMPPPRPTGGPPP"
     gene            complement(3814..4692)
                     /gene="hflC"
                     /locus_tag="VGC27_06970"
     CDS             complement(3814..4692)
                     /gene="hflC"
                     /locus_tag="VGC27_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY0282346.1"
                     /translation="MSRTVTIAVSVAVLFVLGVLFSCVFTIYQTQQALIVQFGAPKAI
                     VREPGLHFKLPWQQVIFFDRRLLNLDAKSEEVIAQDKKRIQVDAFARWLILDPLKFYQ
                     TQNTQSQAVSRLTPILSSNIRRVLGSQNFAAMLSARRSQLMHQIRDNMNQDVKTFGII
                     VVDVRISKADLPPENSRAIYQRMQKERQREAAEFRAQGDETAQRIRARAEREVTVIKA
                     EATRESSILRGQGDAEKTRVLGEAYSQDQSFFAFYRSMQAYQDTFADGQSTVVLSPNS
                     DFFKYFGRGPGASGGK"
     gene            complement(4689..5858)
                     /gene="hflK"
                     /locus_tag="VGC27_06975"
     CDS             complement(4689..5858)
                     /gene="hflK"
                     /locus_tag="VGC27_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY0282347.1"
                     /translation="MPWNNQNGNGPGNQPKSPWGQGSSGGGMRPPDFEELLRKVQESL
                     RRYLPAGGLGRGGWTVAGLAVLVLWLLSGIYIVAPSEQGVVLQFGKFIRHTTSGVNYH
                     LPWPIETAYTPEVTRQRQINIGYKMTENGNLSQTDDVPEESLMLTGDENIVNMHFTVY
                     WQIKDANAYLFNVDNPDSAIKAIGESAMREAVGQERMERILTSDREQIQVKVKRLMQQ
                     ALDTYYAGVWVTDVKMQKAEPPDEVRDAYLDVQRALADQDKKRNEAEAYANRIIPQAR
                     GEAAQIVQDAEAYRQQAIAEASGSAKRFLSVYQEYKKAPEITRRRIYLETMSQVLAPM
                     TKVIVDDAAKGVVPYFQLPMQKALAPQPGSRAPQPAPETGGGQNSPPVAEPGSGQ"
     gene            5933..7036
                     /locus_tag="VGC27_06980"
     CDS             5933..7036
                     /locus_tag="VGC27_06980"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY0282348.1"
                     /translation="MLQATRNDVLKALDGVIDPQSGRSVVQQDMIQGLVLKDGHVGFA
                     LEVAPSRGNEAEPLRQECEKAVHVLPGVLSVTAVLTAHRDGEALRPAHNRPQRRPPAP
                     AGIPGVGAIIAVASGKGGVGKSTVAVNLALGLQALGYKAGLLDADIYGPSVPRLLGIT
                     EKPEAADGGKLAPIVKLGLKAMSIGFLVEEDTPMIWRGPMVQSTLMQMMNDVAWAPLD
                     ILVIDMPPGTGDAQLTIVQQVPLKGAVIVSTPQDIALIDARKALAMFRKTQVPVLGII
                     ENMSTFICPHCGHESQIFGHGGARDTAKALDTPFLGEIPLVPAIRETSDAGTPIVAAA
                     PDGPEAKAFLLIAKEVAAKIETSQRAAPKFVLE"
     gene            7061..7690
                     /locus_tag="VGC27_06985"
     CDS             7061..7690
                     /locus_tag="VGC27_06985"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEY0282349.1"
                     /translation="MVATKPQIRSMFATPVCVHFHPVAQEANAELRPLILEKAQNNGN
                     NNRGEGWRSPADFESWGGDQARTLFRVLRDLADSLTATRAGGRVTLDWKITAAATIRQ
                     KGQYQERAARPGAFWAGVYYVDDGYQKSDDEALGGECELADPRGVLPAMIAPLLGFRV
                     PNGLTAGQTETIRPQSGMIILHPAWQPRGERRYDGNAQRIAIEFELAVP"
     gene            complement(7788..8276)
                     /locus_tag="VGC27_06990"
     CDS             complement(7788..8276)
                     /locus_tag="VGC27_06990"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY0282350.1"
                     /translation="MTISLILARADNGVIGSKGGLPWRLPDDLRRFKSLTMGKPCVMG
                     RKTWESLPRKPLPGRTNIVVTHDRAFAGEGAVVAHSFEEALARAEEENADEIMVIGGA
                     EIFAAAMARAERIHLTEIHAAPEGDVRFPAFAPQQWRETAREDRAQGLAHSYVTLDRK
                     IS"
BASE COUNT         1478 a         2714 c         2748 g         1514 t
ORIGIN      
        1 atatcggcaa gaccctcggt gagcgccgcg aagtagagtc cggtgccgcc gacgaagatc
       61 ggcagacggc cgagcgcccg cgcttcggcc agcgcggcca aggcgtcggt ctgatagcgg
      121 ccgaccgaat agcgctcgga cgccccgaca tggccgtaga gcagatgcgg agcgcgcgtc
      181 ctgtccgcag cggacgggcg cgcggagaga atgcgcactt cgcgataaac ttgcatcgag
      241 tcggcattga cgacgacgcc atccaccgct tcggccagcg ccaaggccgc ggcagacttg
      301 ccgctggcgg tcggccctgc aataagcacc gcatcgaacg ggaggcggat ttggctcaag
      361 aattctccca tgtgctgaat gtgatctcaa agaacgcagg gccgttggcg gtggacatcg
      421 gccgatggct tggcccctgg ctcggtcagt cccctttgag ctggctctcg ccggactatg
      481 ctttcgatgc ccccttcacc ggcgacgcca agaaggctct gacggaagcg cgcgcccaga
      541 ccaagggcct ggcggcggac atcaatgtgg tcgcgacggc cggccgccgc aagaggctgc
      601 tcatcgccga catggattcg accatcatca actgcgagtg cctcgacgaa ctggccggca
      661 tcgccgggct gaggaagaag gtcgccggaa tcaccgagcg cgccatgcgc ggcgagatcg
      721 aattcaccgc cgccttgcgc gagcgcctgg gattgctgaa agggctgccg ctggaggcgc
      781 tggagcgcgt ctggcacgac cgggtgcgcc tcaatccggg cgccaaggaa ttgacggcga
      841 cgatgaaggc caacggtgcg cgcacgctgc tggtttccgg cggctttacc ttcttcaccg
      901 ggcgcgtggc cgaagccgcc ggcttcgaca gccatcagtc caatgtgttg tacgacgacg
      961 gcaaggcgct gaccggagaa gtgcgcgagc cgatcatggg acgcaccgcc aaattgcagg
     1021 cgctggaacg cgcgctccag caatatgggc tgaagccgca ggacgttttg gcggtgggcg
     1081 acggagcgaa cgatctggag atgatccagc gcgccggtct cggcgtggcc tatcacgcca
     1141 agccggtggt cgcggccgcc gcgagcgcca gcatcgcgca tggcgatctg aaggcgctgc
     1201 tctatttgca gggttatcgg gaagaagaaa tcgtttcttc ttgaaatgtc gtggcacggc
     1261 cgcactttgc acaggccggg gtacaggcca cgacaagcga gaattcaatt cgcaatttct
     1321 ttatctcaat ctcagcgcca cgaaggccag cgcgccgccg cggttgacca gcagtatctc
     1381 gactttcctg cccgccttga catcgctttc gagctggcgc tggaaatcgg ccggcgtgcg
     1441 cacgggccgg tcctgcatcg aaacgatcac gtcgcccgcc tggagattct gctcggccgc
     1501 ttggctgtcg ggaatcacgg aggtgacgac gatgccctgc acgccgccgg gcagcttgaa
     1561 ctcggcgcgc gcgccgctgt ccaccggcga cagcgacagt ccgagttgcg acagcttctt
     1621 cttcttggcc ggagtgggcg gctggggttt ggcgccgccc tcgctgagac gcacgaccgt
     1681 cagtcgcacc gtgatctttt tgcccttgcg cagcagatcg acgttcaccg tcttgccgat
     1741 ctgggtgtcg gcggcgaggc gcgacaggct gcgcggatcg gcgatcctct tgccgtcgaa
     1801 ggcggtgatg acgtcgccgt tcctgatgcc ggccttggcg gcggggccgt tcggcgtgac
     1861 gtcggccacc agcgcgccgc cggcggcggg cagcctcatg ccttcggcga tgtcccgcgt
     1921 caccggctgg atgcggatgc cgacccagcc gcggcgcacg ctgccgaact ggcgaatctg
     1981 gccgatcacc tgacgcgcca tgttggacgg gatcgcaaag gcgatgccga ccgaaccgcc
     2041 cgtcggcgag aagatcgccg agttcacccc caccacattg ccgctcatgt cgaacagcgg
     2101 cccgccggaa ttgcccttgt tgatcggcgc gtcggtctgg atgaaatcgt catagggacc
     2161 ggcttcgata tcgcggttgc gcgccgagac gatgccggcg gtgaccgtcg agcccagccc
     2221 gaagggattg ccgatggcga tcacccattc gccgatgcgg gtcttgtcgg agtcgccgaa
     2281 gcgcgccacc ggcaacggct tgcgcgcgga gactttcagc agcgccaggt ccgtcttctc
     2341 gtcgcggccg atcagcttgg cggggagcgt ggtgccgtcg ttcagcgtca ccgcgatctg
     2401 ctcggcgttc tcgaccacat ggttgttggt gacgatcaac ccgctctgat cgatgatgaa
     2461 gccggtgccg agcgaggtga cgtggcgcgg ggcgccgcgg ccggggccga ggaagttctt
     2521 gaacagatcc tgcaggggcg aaccgggcgg caaatccggc agcggcgatt tctggttcgc
     2581 tttcagcgtc tgcgtggtgg cgatgttgac gaccgtgggc aacagcttgg cggacagatc
     2641 ggcgaaggaa tcaggcgcgc cccgcgcgcc cgcaggcatc gccgcgaatg ccaaagaagc
     2701 caataaaaca gccgcaaaac caaacgatcg catgcccatc aacggcgctt tccgacgcga
     2761 ggagggagtt tcgcgcagca tatgcgaata gtttgtgaaa caaagagggc aaacgactta
     2821 tatttcgcgt gatgcctttg cgcccggcgc aggcgcgagc gcccgatgcg cttgccgcac
     2881 gaaaaggcgg accctggcac gcaacgcgcg caccttcgcc acgtggcggc cggccttgaa
     2941 ggcattgcgg ttgcccctgg gcgcgccgcc cccgttgctg atccgcgatt tcggcctttt
     3001 cggtaagctg ctcattttat tgcatttacg ccttgaggtt gttttcgact ttctctctgg
     3061 ggtgggggta ccccctccca ttgcctccgg ctgccggcag gccacgattg agcatgcatt
     3121 atacggccgt tccggcgagg ccggataagt cgggctaaat gcctgtcgcg gcacgactaa
     3181 tggcggggat attccgtacc ttttagcgag aatccgcccc acgcagcagg ttgacacaaa
     3241 gccgcgagcg ccggaggaaa caaaaaggcg aactttcgtc cccttttttc ctcccctatt
     3301 tacgggggag gtccgcccgt agggcgggga gggggcatat ggaggcagat gccctcaagc
     3361 acgctgtcga ggcgtttgta gacatcctcg ttggtcaccc gaaacaggcg ccaaccgcgt
     3421 gatttcaaaa aagcgtcgcg gtgacgatca tggtcgagtt cggcatctgt agaatgtgtg
     3481 ccgccgtcga cttcgacaat gaggcgggtc atcgcgcagg caaaatccgc aatgtaaggg
     3541 ccgataggat gctgtcggcg gaatcgaaac ccctcggcgg cccgcccgcg aagccgcgac
     3601 catagaatca cttcggcatc ggtcatatgc cgtcggagtt ttttcgcgcg cttggttgtt
     3661 cttcgctcgg aatcgcgcat gccccctccg gtcctgcgga ccacctcccc cgtaaacggg
     3721 ggaggaaaga gagaatacta ttcccgcttt ccgccgccgg aagcgccagg gccctgcccc
     3781 gccttcgcca aagccacggc gggcgccccg gccttatttg ccgcccgaag ctcccggacc
     3841 cctgccgaaa tatttgaaga agtcgctgtt gggcgaaagc accacggtac tctggccgtc
     3901 ggcgaaggta tcctgatagg cctgcatgga gcggtagaaa gcgaagaaag actggtcctg
     3961 gctgtaggcc tcgcccagaa cgcgggtctt ctccgcgtcg ccctggccgc gcaggatcga
     4021 ggactcgcgc gtggcttcgg ccttgatcac cgtcacttcg cgctcggcgc gggcgcggat
     4081 gcgctgggcg gtttcgtcgc cctgggcgcg gaactcggcc gcttcgcgct ggcgttcctt
     4141 ctgcatgcgc tgatagatcg cccggctgtt ctccggcggc aggtcggcct tgctgatccg
     4201 cacgtcaacc acaatgatgc cgaaggtctt cacgtcctgg ttcatgttgt cgcgaatctg
     4261 gtgcatcagc tgggagcgcc gcgccgacag catggcggcg aagttctgcg aacccaggac
     4321 gcggcggatg ttggaggaca gaatcggcgt cagccgcgac accgcctggc tctgcgtgtt
     4381 ctgcgtctgg tagaatttca gcggatcgag gataagccag cgcgcgaagg catcgacctg
     4441 gatgcgcttc ttgtcctggg cgatcacttc ctcgctcttg gcgtcgagat tgagcagccg
     4501 ccggtcgaaa aagatgacct gctgccaggg cagcttgaag tgcaggcccg gctcgcgaac
     4561 gatcgccttc ggcgcgccga actgcacgat cagcgcctgt tgggtctggt agatcgtgaa
     4621 gacgcaggag aacagcacgc ccagcacgaa gaggacggcg acggagacgg cgatggtgac
     4681 ggttcggctc attgtccgct ccccggttcg gcgacgggcg gcgagttctg gccgccgccg
     4741 gtttcaggcg ccggctgcgg cgcgcgcgat cccggctgcg gcgccaacgc cttctgcatc
     4801 ggaagctgga agtagggcac cacgcccttg gccgcgtcgt ccacgatcac cttggtcatc
     4861 ggcgccagca cctggctcat cgtttccaga tagatgcgcc gccgcgtgat ctccggggcc
     4921 ttcttgtatt cctgatagac cgaaaggaag cgcttggcgc tgcccgaggc ctcggcgatg
     4981 gcctgctggc gatacgcctc ggcatcctgc acgatctggg cggcctcgcc gcgcgcctgc
     5041 gggatgatcc ggttggcgta ggcctcggct tcgttgcgct tcttgtcctg gtcggccagc
     5101 gcccgctgca cgtcgagata ggcgtcgcgc acctcgtcgg gcggctcggc cttctgcatc
     5161 ttcacgtcgg tcacccagac gcccgcatag taggtatcga gcgcctgctg catcagccgc
     5221 ttgaccttga cctgaatctg ctcgcggtcg gaggtcagga tccgttccat gcgttcctgg
     5281 ccgacggcct cgcgcatcgc gctttcgccg atcgccttga tggccgaatc gggattgtcg
     5341 acgttgaaca gataggcgtt ggcgtccttg atctgccaat agaccgtgaa gtgcatgttg
     5401 acgatgttct catcgccggt gagcatcagg ctttcttcgg ggacgtcgtc cgtctgcgac
     5461 agattcccat tctcggtcat cttgtagccg atgttgatct gcctctggcg cgtgacctcg
     5521 ggcgtgtacg cggtctcgat cggccacggc aggtggtaat tgacgcccga agtggtgtgg
     5581 cggatgaact tgccgaattg cagcaccacg ccctgttcgg acggagcgac gatataaatg
     5641 ccggacagca gccacaggac cagaaccgcc agcccggcca ccgtccagcc gccgcggcca
     5701 agtccgccgg caggcaggta gcgcctcagg ctttcctgca ccttgcgcaa aagctcctcg
     5761 aaatcgggcg gacgcatgcc gccgccggag gaaccctgtc cccagggact tttgggctgg
     5821 ttgcccggac cgttgccgtt ctgattattc cagggcattc gcgtatcctg cctgggtttc
     5881 ttgtattccg tgcctgcccg gttatatgag catccttccc aaggcgcaaa ccatgcttca
     5941 agccacacgc aacgatgttc tgaaggcgct cgacggcgtc atcgatccgc aaagcgggcg
     6001 cagcgtcgtg cagcaggaca tgattcaggg cctggtgctg aaggacggcc atgtcggatt
     6061 cgccctggaa gtcgcgccct cgcgcggaaa cgaggccgaa cccttgcgcc aggaatgcga
     6121 aaaggcggtg catgtcctgc ccggcgtgct gtcggtcacc gccgtgctga ccgcgcaccg
     6181 ggacggcgaa gccctccgcc ccgcccataa ccgcccgcag cgccgcccgc ctgcgccggc
     6241 cggcattccc ggcgtcggcg ccatcatcgc ggtcgccagc ggcaagggcg gcgtcggcaa
     6301 atcgacggtg gcggtgaatc tggcgctggg cctgcaagcg ctgggctaca aggccgggct
     6361 gctcgacgcc gacatttacg ggccgagcgt gccgcgtctc ctgggcatca ccgaaaagcc
     6421 cgaggcggcg gacggcggca aactggcgcc catcgtcaag ctcggcctca aggccatgtc
     6481 gatcggattc ctggtcgaag aagacacgcc gatgatctgg cgcggcccga tggtgcaaag
     6541 taccctgatg cagatgatga acgacgtggc gtgggcgccg ctggacatct tggtgatcga
     6601 catgccgccc ggaaccggcg acgcacagct caccatcgtc cagcaggtgc cgctgaaagg
     6661 cgcggtgatc gtctccacgc cgcaggacat cgccttgatc gatgcgcgta aggccctggc
     6721 gatgttccgc aagactcagg ttccggtcct gggtatcatc gagaatatga gcaccttcat
     6781 ctgcccgcat tgtgggcacg aaagccagat tttcggtcat ggcggcgcgc gcgatacggc
     6841 aaaagcgctg gacacgccgt ttttgggcga aattccgctg gtcccggcca ttcgcgagac
     6901 ctcggacgcc ggcacgccaa tcgtcgcagc cgcccccgac ggtcctgaag ccaaagcttt
     6961 cctgttgata gccaaggaag tggcggcgaa aatcgaaaca tcgcaacgcg ccgccccgaa
     7021 atttgtgctg gaatagaccg agaagcaatt tcagtagccc atggtagcca ccaaacctca
     7081 aatccgcagc atgttcgcga cgccggtgtg cgtccatttc catcccgtgg cgcaggaggc
     7141 caacgccgaa ctgcgcccgc tcatcctgga aaaggcgcaa aacaacggca acaacaaccg
     7201 cggcgaaggc tggcgttcgc ccgccgattt cgaaagctgg ggcggcgatc aggcgcggac
     7261 cttgttccgc gtgctgcgcg atctggccga cagcctgacg gcgacgcgcg ccggcgggcg
     7321 cgtgacgctc gactggaaga tcaccgccgc ggcgacgatc cgccagaaag gccaatatca
     7381 ggaacgcgcg gcgcgtccgg gcgccttctg ggcgggcgtc tattacgtgg acgacggcta
     7441 ccagaaatcc gacgacgaag cgctcggcgg ggaatgcgaa ctcgccgatc cgcgcggcgt
     7501 gctgccggcg atgatcgcgc cccttctcgg cttccgcgtg cccaacggtc tgaccgccgg
     7561 gcagaccgaa accatccgcc cgcaatcggg catgatcatc ctgcacccgg cctggcagcc
     7621 gcgcggcgag cgccgctatg acggcaatgc ccagcgcatc gccatcgagt tcgaactggc
     7681 cgtgccgtaa tcgtgagatt cgccggcacg gccgttgcga agcgcagaac ttatttctgg
     7741 atgcccggga ccagttcgta gtcgcgcccg ccgcccaccg gcttccgcta ggatattttg
     7801 cggtcgagcg tcacataaga gtgggcaagc ccctgggcgc gatcctcccg tgccgtttcc
     7861 cgccattgtt gcggcgcgaa agcgggaaac cgcacatcac cctccggcgc ggcgtggatt
     7921 tccgtcaggt ggatgcgttc cgcgcgcgcc atggcggcgg cgaaaatctc cgcgccgccg
     7981 atgaccatga tctcatccgc attctcttct tccgcgcgcg ccagggcctc ttcaaacgaa
     8041 tgcgcgacga cggcgccttc gccggcaaag gcgcggtcat gcgtcaccac gatattggtg
     8101 cggccgggca gcggctttct gggcaggctc tcccaggtct tgcgccccat gacgcagggc
     8161 ttgcccatgg tcagagattt gaagcggcga agatcgtcgg gcaaacgcca gggcaagccg
     8221 cccttgctgc cgatgacgcc gttgtccgcg cgcgcgagga tgagggagat ggtcatagtc
     8281 caccatccgt acgctgccgc ttcggaccct tcgagacgcc cccttcacac cgcgaaggag
     8341 ccttaggatg agtgtggcta atactatgtt cctcatcctg aggaggccgc gcagcggccg
     8401 tctcgaagga tgaggagcgt cgccgcgcaa gttttgacag ctcgatgtag tctc
//
LOCUS       DASWPU010000325         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_113135,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000325 DASWPU010000000
VERSION     DASWPU010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113135"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VGC27_06995"
     CDS             <1..1075
                     /locus_tag="VGC27_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0282351.1"
                     /translation="NTTAYLEADLEHDLIGRPLKLNAGVRWVQVRDSMTFWDHTQFLV
                     DGSHNSTTGKAYVAKFLPSATLSYEPTDDLKFRLNYGETLRRPDFTALNPTYILNADV
                     TGVGYATGTRGNPDLKPTQSKNLDLTGEWYFAPNSAIYSTLFKRDIEGLVVNQNVALV
                     FPGDAIKDQYNTNNFIINEPMNASKGHLKGVEVGLVYFPDFLPGVLDGLGAQGSLTIL
                     SSSQNVPQTDSLGNVVSQLEQEFFGVSHTSYNATLIYERGDIGARLSYVWRSKFHYDN
                     EAASFANPLGRWHTAESSLDLQLSYKVWDNAMVTFDAVNLLNGVQQQYYYGGGSSGGP
                     TVADFGTLLISRTFSVGLRYSLN"
     gene            1520..2452
                     /locus_tag="VGC27_07000"
     CDS             1520..2452
                     /locus_tag="VGC27_07000"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HEY0282352.1"
                     /translation="MTGAISPIHDPCIIKDGDTYYVFCTTMDDKKQAQIPVHTSKDLI
                     HWKAAGYAFPKIPDWALKMIPATKGIWAPDISYRGGTYYLYYSLSSFGSIHSVIGCAT
                     STTLDPKSPQYKWVDHGLVFMSDKGSSFNCIDPAHIFDREGNRWLAFGSFWTGIKMIR
                     LDPKTGKQSQADKTIYTLAARPIPEGGLDTVEACFLIERGGWYYLFASYDYCCKGVNS
                     TYYTAMGRCKNITGPYLDKQGRKMTEGYGWVILKADQEEQGRWRGPGHCAVLRDPGQD
                     YIVYHAYDKLHKGAPYLRIAPLVWSADGWSTAVV"
     gene            2494..>3140
                     /locus_tag="VGC27_07005"
     CDS             2494..>3140
                     /locus_tag="VGC27_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282353.1"
                     /translation="MNDLKPIRLIALASIAILSISAASIAQSPQTVSATVTIHADKPG
                     ATINKNIYGQFSEHLGTGIYGGIWVGEDSTIPNTKGFRNDVIAALKDLRVPVVRWPGG
                     CFGDEYHWREGVGPRAQRAVRVNHNWGGVPENNAFGTHEYFDFVDMIGSDSYVSANVG
                     TGSPAEMADWLEYMTTDQDTTLAHARKANGRDKPWKLDFLAIGNETWGCGGNMTA"
BASE COUNT          675 a          969 c          895 g          601 t
ORIGIN      
        1 gaacaccacg gcctacttgg aggccgatct ggagcacgat ctcatcgggc ggcccctcaa
       61 gctgaatgcg ggcgtgcgct gggttcaggt cagggacagc atgaccttct gggaccacac
      121 gcagttcctg gtcgacggct cgcataactc gaccacgggt aaggcttacg tggcgaagtt
      181 cctgccaagc gccaccttgt cttacgagcc gacggacgac ctcaaattcc gcctcaacta
      241 tggcgaaacg ctgcggcggc cggatttcac ggcgctcaat cctacttaca tcctcaacgc
      301 ggacgtgacc ggcgtcggct acgccacggg aacgcgcggc aacccggatc tgaagcccac
      361 tcaatccaag aacctcgacc tgaccggcga atggtatttc gcgcccaaca gcgcgattta
      421 cagcacgctg ttcaagcgtg acatcgaagg actggtggtc aaccagaacg tcgcgctcgt
      481 cttcccgggc gacgcgatca aggatcagta caacaccaac aacttcatca tcaacgagcc
      541 gatgaacgcc tcgaaaggtc atctgaaggg cgtggaagtg ggtctcgtct atttcccgga
      601 ctttctgccg ggggtcctgg acggcctcgg cgctcaaggc agcctgacga tcctcagctc
      661 gtcgcaaaac gttccgcaga ccgattcgct gggcaacgtc gtttctcaac tggaacagga
      721 attcttcggt gtctcccaca cctcctacaa tgccacgctg atttacgaaa ggggcgacat
      781 cggtgcgcgc ctctcctatg tctggcgtag caagttccac tacgacaacg aagcggcctc
      841 gttcgcgaat ccgctggggc gctggcacac cgccgaatcg agcctggacc tacagttgag
      901 ttacaaggtc tgggacaatg ccatggtgac gttcgatgcg gttaacctgc tgaatggcgt
      961 gcagcagcag tattactacg gcgggggcag cagcggcggg ccaaccgtcg ccgacttcgg
     1021 cacgctgctc atcagccgga cgttctcggt tgggctccgc tactctttga actgagacat
     1081 ctaaaaggca cccggccgca cgcattcgcg gccgggtgtc gttttccgga tcgatgcgaa
     1141 aggaccgtat tgccaaacca tcgagttggg tctgcgttac agactgcaag tgcagcgcgt
     1201 acctcgggag taacatgagc caagtttgat attcaacgtc ggcttggccc cgtcacttcc
     1261 agaggatttg gttcgtagct ttaactctct cctggatgaa cggtggcggt ttgggcgtta
     1321 agacgtgtct ccgccgccgc cgttttcttc aaatgacagc aagcgggtca aaaagatgca
     1381 gttggaaaag acggaacatc tcctcaaaac catcaaccgc cgcagagcgc tgcaaggttc
     1441 gctatcgctc ggattcgcca caacggttcc tttcgcttcc gctgtctcgg cgccggcgcc
     1501 aaagtccata aacgagcgga tgactggcgc catcagcccg atccacgatc cctgcatcat
     1561 caaggacggc gacacctact acgtcttctg cactacgatg gatgataaga aacaggcgca
     1621 gattccggtc cacacttcca aggatttgat tcactggaag gcggccggtt acgccttccc
     1681 caagattccg gattgggcgc tgaagatgat cccggcgacc aagggcatct gggcgccgga
     1741 tatcagctat cgaggcggca cctattacct ctattactcg ctgtccagtt ttggttcgat
     1801 ccattcggtg atcggctgcg ccaccagcac cacgctcgat cccaaatcgc cgcaatacaa
     1861 atgggtggat cacggcctgg tgttcatgtc cgacaagggc agcagcttca actgtatcga
     1921 cccggcgcac attttcgacc gcgaaggcaa ccgctggctc gccttcggca gtttctggac
     1981 cggaatcaag atgatccggc tcgaccccaa gaccggcaag caatcgcagg cggacaagac
     2041 catctacacg ctggcggcgc gtcccatacc cgaaggcgga ctcgataccg tcgaggcctg
     2101 cttcctcatc gaacgcggtg gttggtacta cctcttcgcg tcctacgact attgctgcaa
     2161 gggcgtgaac agcacctatt acacggcgat gggccgctgc aagaacatca ccggacctta
     2221 tctcgacaag cagggccgca agatgacgga gggctatggc tgggtcatcc tcaaggccga
     2281 tcaggaagag cagggccgtt ggcgcggccc cggccattgc gcggtcctgc gcgatcccgg
     2341 ccaggactac atcgtctacc acgcctacga taaattgcac aaaggcgcgc cgtatttgcg
     2401 gatcgcaccg ctggtgtggt cggcggacgg ttggtccacc gccgtcgttt agcggctcgg
     2461 caaggaacca gaacaacgac aggagaaaac gccatgaacg atctcaagcc cattcgcctt
     2521 atcgccctgg cctcgatcgc gatattgtcg atatcggccg cgtccatagc ccaatccccg
     2581 cagaccgtct ctgcgaccgt gaccatccat gccgacaagc ccggcgcgac gatcaacaag
     2641 aacatctacg gccaattctc cgaacatctt ggcaccggca tctatggcgg tatttgggtg
     2701 ggcgaggatt cgaccattcc caacaccaag ggtttccgca acgacgtaat tgcggcgctc
     2761 aaggatttgc gcgtgccggt cgtgcgctgg cccggcggct gtttcggcga tgaataccat
     2821 tggcgcgagg gcgtcggccc gcgcgcgcaa agagccgtca gggtcaatca caattggggc
     2881 ggcgtgccgg agaacaatgc gttcggcacc catgaatatt tcgatttcgt cgacatgatc
     2941 ggttccgact cctatgtcag cgccaatgtc ggcaccgggt cgccggcgga aatggcagac
     3001 tggctcgaat acatgaccac cgaccaggac acaacgctgg cccatgcgcg caaggcgaac
     3061 gggcgcgaca agccgtggaa gctcgacttt ctggcgatcg gcaacgaaac ctggggctgc
     3121 ggcggcaaca tgacggccga
//
LOCUS       DASWPU010000326         3986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_51665,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000326 DASWPU010000000
VERSION     DASWPU010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51665"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VGC27_07010"
     CDS             complement(<1..544)
                     /locus_tag="VGC27_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0282354.1"
                     /translation="MIQRISVLPGHGRDGQPDRVSRLDLEMGDVVSVVGHTGSGKSTL
                     INDIELFANRDTPTGRTVLIDDKPVPEEYRNDPSCNPIALITQHTAFLSDLPVGEFLT
                     AHARIRKRTKSEIEQALELTLRFANELTGEPITPDIRMTELSGGQTRAILIADATVIC
                     NTPIVLLDEVENAGIHRGRAL"
     gene            complement(1114..3762)
                     /locus_tag="VGC27_07015"
     CDS             complement(1114..3762)
                     /locus_tag="VGC27_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0282355.1"
                     /translation="MKRHLLGTSAIALVLASGSPVYAQANTQVADARSETVETVIITG
                     THLQDRTLTNTPVPVDVLTGEELRQSGQSETTEILRQVAPSFSFATPTTPDGNTHIHS
                     ASLRGLSPDETLVLINGKRVHGAGWVNTGGTIGKGAVPVDLNQIPSAAIGRIEILRDG
                     ASSQYGSDAIAGVINIVLREDTALHATASAGTTQDGGGFTYDVSAGDGFKLGSDGFLN
                     LTAYYRDHKAANRALPDTRQFYFGNAPNGSLQPLSARWGSGVGINPPGGVAGTTLDPR
                     EATVDRDVWRFADEGDLKDASVIANFNKPLGNGIEFYGFAGYRVSKAHSNASFRRAGQ
                     DENVRAIYPDGFLPFINTTSTDYSISVGLKGDVAGWAWDLSTIYGGNGVAYRTENTLN
                     ATLGTASPTQFYNGEYSNRQSTTDLVFSREFQVGLAGPVSVAVGAEFRYDGYKIKPGE
                     PDSYTFGTSRVLDGPNAGAVPTIGSQGFAGIQPGDAVDADRHNVSVFTEFGFVPVEGW
                     QVNLAGRYENYSDFGSTTNGQVSTRLELFGGLAARASVSTGFHAPALAQQYFSSTSSR
                     TIVNNTTGFPEYVLVRTAPVGSALAKALGAVDLKPEKSVNFSGGFVFAQGGFSASVDY
                     YHIDIDDRIFLSSNYVDASGSRVLRNYLASIGIPGVTSVRYFTNAADTRTQGVDVTLS
                     YSADLADYGSLRLTGGYNYNETRLRNVAATPSQITSLGIRTPLFDVIERTRVERGQPR
                     DKVALGGNWEIGRFTINLHGTRYGRVEQVALTNQNAANVALVALGNTPYRTLPTESGS
                     AGNSDVIQKLDPRWVTDLSVTAQISETIDLTLGANNLFNVYPTKNIKTTASLTGADTY
                     GVFPYSEFSPFGFSGAYYFARVGLSL"
BASE COUNT          783 a         1163 c         1245 g          795 t
ORIGIN      
        1 ccagggcgcg gccgcgatga atgcccgcgt tctcgacttc gtcgagcaga acgatcggcg
       61 tgttgcagat gaccgtcgca tccgcgatga ggatggcgcg cgtctgtccg ccggaaagct
      121 cggtcatgcg gatatcgggg gtgatcggct cgccggtgag ttcgttcgca aacctcagcg
      181 tcagctccag cgcctgctcg atctccgatt tggtgcgctt cctgatgcgc gcgtgggccg
      241 tcaggaactc ccccaccggc aggtcggaga gaaacgccgt atgctgggtt atcagcgcaa
      301 tgggattgca cgacgggtcg ttgcggtatt cctccggcac cggcttgtcg tcgatcagca
      361 cggtccgccc cgtcggcgtg tcgcgattgg cgaacagctc gatgtcgttg atcagggtcg
      421 acttgccgga acccgtgtgc ccgacgacgc tgaccacgtc gcccatttcg aggtccaggc
      481 gcgacacccg gtccggctgg ccgtcccggc cgtgccccgg cagaaccgaa atacgttgaa
      541 tcatcgccca actcccacag ctatactata ctatatcgat gtatataaca ttatttaaat
      601 gtattttgga ccagtttaga ggcaacatgt cgcgtcgcgc accggatatt accggagtac
      661 cggcggcaac agcaatttta taagacgatt tgaagaagtg ttgtcagcaa aagaggttgc
      721 gcagcagccg caacagttca agcgccgggg atcggcgatg cgatcgtaaa tcgtctgcgc
      781 cttgcgcctt aagccggacg cccgcacaag ctcacccacg gagcgctccc cagcaccaga
      841 ccgtaaagca cgttcaatcg ttcggcgtgc gaaagcgcct gcaacagggc ggcggcatct
      901 tcggccttgc ggccatcgcc ttcaggttgc cgtgaacgtt ctttgtccga tgggccgggc
      961 gcgattccgg ctgtgcggcg tcggcttcaa tacatacaac tcgaacaatt ccgccatcgt
     1021 cgccgtgtac ggcacgcacc acgatgttcg gcgtctctta gtgtcattga attgctcatc
     1081 gataatcggg ctgaataccg gcattgagta gagtcagagg ctcagaccga cgcgggcaaa
     1141 ataataggcg ccgctgaagc cgaagggtga aaactcgctg tacgggaaaa ccccgtaggt
     1201 gtcggcgccg gtcagcgatg ccgtcgtttt gatattcttg gtcggataga cgttgaagag
     1261 gttgttcgcg cccaaggtca agtcgatggt ttcggagatt tgtgccgtca cgctcaaatc
     1321 ggtgacccat ctgggatcga gcttctgtat gacatcggaa ttgccggcgc tcccggattc
     1381 ggtggggagc gtgcggtacg gcgtgttgcc gagcgccacc agcgccacat tggccgcatt
     1441 ctggttcgtc agagccacct gctcgacccg gccgtaacgc gtgccgtgca gattgatcgt
     1501 gaagcgtccg atctcccagt taccgccaag cgcgaccttg tcgcggggct ggccgcgctc
     1561 cacccgcgtg cgctcgatca cgtcgaagag cggagtcctg atgccgaggg aggtgatctg
     1621 gctgggcgtg gccgcaacgt tgcgaagccg cgtctcgttg taattatagc cgccggtcag
     1681 ccgtaagctg ccgtagtccg caagatccgc actatagctc agcgtgacat cgacgccctg
     1741 ggtgcgcgta tcggcggcgt tcgtgaaata gcgcacgctc gtcacgcccg ggatgccgat
     1801 cgaggcgagg taattgcgca agacccggct tcccgaggca tcgacatagt tgctcgacag
     1861 gaagatccga tcatcgatgt cgatgtgata gtaatcgacc gatgcgctga acccgccctg
     1921 cgcgaagacg aacccgccgc tgaaattgac cgacttttca ggcttcagat cgacggcgcc
     1981 gagcgctttg gcgagcgcag agccgaccgg cgccgtcctg acaagaacgt attccggaaa
     2041 gcccgtcgtg ttattcacga tcgtgcggct gctggtcgag ctgaaatatt gctgggccag
     2101 agcgggggcg tggaagccgg tgctgaccga ggcgcgggca gcaaggccgc cgaagagttc
     2161 gagccgggtc gatacctggc cgttcgtcgt cgaaccgaag tcgctgtaat tctcatagcg
     2221 gcccgccagg ttgacctgcc agccctccac cggcacaaag ccgaattcgg tgaacacact
     2281 cacattgtgg cgatcggcat caacggcatc ccccggctgg attccggcga atccctgcga
     2341 tccgatcgtc gggacggcgc cggcgttggg accatcgaga acgcgcgacg ttccgaaagt
     2401 gtagctgtcc ggctcgcccg gtttgatctt gtaaccgtcg taacgaaact cggcaccgac
     2461 ggcgacgctg accggccccg ccagcccaac ctggaattcg cgcgaaaaga ccagatccgt
     2521 ggtggattgc ctgttgctgt attcgccgtt gtagaactgc gtcgggctgg ccgttccaag
     2581 cgtcgcgttg agggtgtttt cggtccggta ggcaacgccg ttgccgccat agatcgtgga
     2641 caggtcccac gcccagcccg cgacatcacc tttaagaccg acgctgatgg aatagtccgt
     2701 gcttgtggta ttgatgaagg gaagaaagcc gtcgggatag atggctctga cattctcatc
     2761 ctggcccgcc cggcggaacg acgcgttgga atgcgccttg ctgacgcgat agcccgcgaa
     2821 gccgtaaaac tcgatgccgt tcccgagcgg tttgttgaaa ttggcgatga cgctggcgtc
     2881 cttcagatcg ccttcgtcgg caaagcgcca gacgtcgcga tcgaccgtcg cttcgcgcgg
     2941 atcaagcgtg gtgcccgcaa cgcctcccgg cgggttgatg ccgacgccgg atccccaacg
     3001 ggccgaaagc ggctgcaaag atccgttggg cgcatttcca aaatagaatt ggcgcgtgtc
     3061 gggaagcgcg cggttcgcgg ccttgtgatc gcggtaatag gcggtcaggt tgagaaaacc
     3121 gtcgctgccc agcttgaagc cgtcaccggc tgacacgtcg taggtgaagc cgccgccatc
     3181 ctgggtcgtg cccgcactcg ccgtcgcgtg aagcgcagtg tcttcgcgca atacgatgtt
     3241 gatgacgcct gcgatggcgt cggagccata ttgcgaagag gccccgtcgc gcaggatttc
     3301 gatgcggccg atggcggccg agggaatctg gttgaggtcc acgggtacgg cgcccttgcc
     3361 gatcgttccg ccggtattta cccagcctgc cccatgcacg cgcttaccgt tgatcagcac
     3421 cagcgtctcg tcgggcgaca ggccgcgcag actggcggaa tggatgtgcg tattgccgtc
     3481 aggcgtggtc ggagtggcga aagagaagga tggcgcaact tgccgcagga tttccgtggt
     3541 ctcgctttgg ccggactggc gcagttcctc gccggtcagc acgtcgacgg gcacgggggt
     3601 attggtcagc gtccgatctt gcagatgcgt gccggtgatg atgacggttt cgacggtctc
     3661 ggaccttgca tccgccactt gcgtattggc ttgcgcgtaa accggcgaac cactcgcaag
     3721 taccagcgcg atggccgatg tgcccaacag atgacgcttc atttcaatca cccctcacaa
     3781 aattccacac gacaaacgcc gtaatatatt taatctatag aaattataga gatgatgaaa
     3841 tgtgacaaat tgatttcaca tcgcgagtat gtgcaaggga atgtcggctg gtctattgga
     3901 gggcggagat cggctgcgcg ttcgaattcg agacgcggca agagaagcaa gagaaggaag
     3961 agtgcaatga tcaacgaatt aacgcg
//
LOCUS       DASWPU010000327         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_64242,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000327 DASWPU010000000
VERSION     DASWPU010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64242"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..436)
                     /locus_tag="VGC27_07020"
     tRNA            complement(344..436)
                     /locus_tag="VGC27_07020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(400..402),aa:Ser,seq:gga)
     gene            complement(526..954)
                     /locus_tag="VGC27_07025"
     CDS             complement(526..954)
                     /locus_tag="VGC27_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282356.1"
                     /translation="MFRVFPLMLIPVALYNLIVLFGNVIANNDMFHLLTKSFTVDMVS
                     GEWAVSFSDVVLLLAILMLFIEVIKSSGTSRREMINNALSILVFVVALIEFIMLKGFA
                     TSAFFFITVLCLFDSIAGPTVTAVAAKRDIGLGSNIIDAG"
     gene            complement(1058..1363)
                     /locus_tag="VGC27_07030"
     CDS             complement(1058..1363)
                     /locus_tag="VGC27_07030"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0282357.1"
                     /translation="MAHALSWITVDHVWLLIGFTGQALFTARFIVQWFKSEIVGKSVI
                     PLAFWWFSVGGGIVLLAYAVHRMDPVFIAGQAGGLVVYARNLYLIFRERKAKRSSAA"
     gene            complement(1365..2084)
                     /locus_tag="VGC27_07035"
     CDS             complement(1365..2084)
                     /locus_tag="VGC27_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0282358.1"
                     /translation="MAMSLSVVVPVKDEAANVGPLAREIAAAVANEPDLEIIFIDDGS
                     SDGTAEALAALKSEIPSLRVIRHAANLGQSRAIRTGVRAARADIVVTLDGDGQNDPAD
                     IPKLLAELRKDKGTGLVSGVRASRQDKLSRKLASRAANSVRQWLLKDNATDAGCGLKA
                     FRRDAFLALPYFDHIHRFLITLMLREGYEVRFVDVNHRPRLHGTSKYTNLNRLLVGVC
                     DLLGVRWLQRRFRGRADAKEM"
BASE COUNT          457 a          678 c          717 g          402 t
ORIGIN      
        1 atggatgctt cgcctcaggc gataggggtt caccatctgt tgatcacaga cgggcaaggc
       61 gaagagcggc ttgccacttt aaactgcgcg aacccgatga cagccctcct acgctctgac
      121 gagcttcgga gggcaccctc cgctgctgcg cagcgtaggg tggcggacag gaagggattg
      181 cgaaaatgcg cttcggcgca ttttcgtcct ctggctgacg cccctcggat cgcttcgctc
      241 cgctccgcgc ccaaaatggc ctgccggcca ttttgtcgaa caagggttcg ttcaccactc
      301 cccgatccgc cggcaaaaaa gggcccacaa gggacccttt tgctggcgga cagggaggga
      361 ttcgaaccct cggaacggct cagcaccgtt cacacacttt ccaggcgtgc gccttcagcc
      421 actcggccac ctgtccgcgg cgcacggatt tataggaatt ggcctgcaaa gcaagccgga
      481 cggcccaaat gggccgtccg gctcacccgc aggcgaaaag cgggcttaac cggcgtcgat
      541 gatgttgctg cctaggccga tatcgcgctt ggccgccacg gcggtgaccg tcggcccggc
      601 aatcgagtcg aacaggcaca gcaccgtgat gaagaagaac gcggaggtcg cgaagccctt
      661 gagcatgatg aactcgatca gcgcaaccac gaacacgagt atcgacaggg cgttgttgat
      721 catctccctg cgggaggtgc cggacgattt gatcacctcg atgaacagca tcaggatcgc
      781 cagcagcagc acgacgtcgc tgaaactcac ggcccattca ccggacacca tatcgacggt
      841 gaacgatttc gtcagcagat ggaacatgtc gttgttggcg atgacgttgc cgaacaggac
      901 gatcagattg tagagcgcga ccggaatgag catgagcgga aacacgcgaa acatggaatt
      961 cctccccaag cgacaagaag ccccaattag cttgtaccat aatagggcga aactggggcc
     1021 cgcatagggc ctccgtgtga cagtatggtt aagggagcta ggccgcgctc gagcgtttcg
     1081 cctttctctc ccggaaaatc aggtagaggt tgcgggcata gaccacgagg ccgccggcct
     1141 ggccggcgat gaagaccgga tccatccggt ggaccgcata agccaagaga acgattccgc
     1201 cgcccacgct gaaccaccag aaggcgaggg gaatgacgct cttgccgacg atctcgctct
     1261 tgaaccactg gacgatgaac cgcgccgtga acagcgcttg gcccgtgaag ccgatcagca
     1321 gccagacgtg atcgacggtg atccacgaga gcgcgtgcgc catatcacat ttccttcgcg
     1381 tcggcccggc cgcggaagcg gcgctgaagc catctgacgc ccaacaggtc gcagacgccg
     1441 accagcaggc ggttgaggtt ggtgtatttg gaggttccgt gcaggcgcgg ccggtggttc
     1501 acgtccacga agcgcacctc gtagccctcg cgcagcatca aggtgatgag gaagcgatgg
     1561 atgtgatcga aatagggcag cgccaggaag gcgtcgcggc ggaacgcctt caggccgcag
     1621 ccggcatcgg tggcgttgtc cttgagcagc cattggcgga cggaattcgc cgcgcgcgag
     1681 gccagcttgc ggctgagctt gtcctggcgg ctggcgcgga cgcccgagac caggccggtg
     1741 cctttgtcct tgcgcagttc cgccagcagc ttggggatgt cggcgggatc gttctgaccg
     1801 tcgccgtcga gggtgacgac gatgtcggcg cgggccgcgc gcacgccggt ccggatagcc
     1861 cggctctggc cgaggttggc ggcgtggcgg atgacccgca aggagggaat ttcggatttg
     1921 agggccgcca gcgcctcggc ggtgccgtcg gacgagccgt cgtcgatgaa gatgatctcc
     1981 aggtcgggct cgttcgcgac cgccgcggcg atctcgcgcg ccagcgggcc gacattggcg
     2041 gcctcgtcct tcaccggcac aaccacggac agcgacatcg ccatgctgag gttccgttcg
     2101 attccatcgt gggctagtgt cccgattccg aaattcgcac tatctttata tcggctccgg
     2161 gcgaatttcg gaatcgaaag gacactagca acctgttgaa tcctagtgtc gttttggttt
     2221 cgaagttcgc ttgcagccag atataacaaa tggt
//
LOCUS       DASWPU010000328         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_94562,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000328 DASWPU010000000
VERSION     DASWPU010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94562"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VGC27_07040"
     CDS             <1..1015
                     /locus_tag="VGC27_07040"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY0282359.1"
                     /translation="LGWTMTDADVSTVRAVNSAAISEAAALLRAGRLVAFPTETVYGL
                     GADATNGRAVASIFVAKGRPHFNPLIVHVKNAEQAREYTVFSSLAEELARAFWPGPLT
                     LVLPRREDCRVSLLVSAGLETMALRAPSHPVAMRLITEAGVPLAAPSANPSGRVSATL
                     AAHVEEGFRGMVDMILDGGPTLIGIESTVIGFENGKPVLLRPGAVPREALERITGPLA
                     VPAQGAIISPGRLASHYAPNAHLRLNALEAAPDESLLAFGNVPGGADAPGGADAPGGR
                     RVLNLSPSGDLGEAAANLFAMLRRLDAESDAIAVMPIPSSGLGEAINDRLMRAAAPRK
                     ITA"
     gene            1012..>1572
                     /locus_tag="VGC27_07045"
     CDS             1012..>1572
                     /locus_tag="VGC27_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0282360.1"
                     /translation="MNDDVAARLKAAAGPKGFTEDPAEIAPHLEEWRSKYKGRATLML
                     KPATTAEVSAILAICNESGTPVVPQAGNTGLVGAQIPFDNEVLLSLSRLNRIRALDAE
                     DASMIAEAGVVLEVAQNAADDKGLMLPLSLAAEGSCTIGGTISTNAGGVNVLRYGMAR
                     EQVLGLEVVLADGRVLDLLRTLRKDNT"
BASE COUNT          260 a          519 c          545 g          248 t
ORIGIN      
        1 gctaggttgg accatgaccg atgctgatgt ttccactgta cgcgcggtaa attccgcggc
       61 gatctcagag gctgcggcgc tgctccgcgc cggccggctg gtggcgtttc caaccgagac
      121 ggtttacggg ctgggcgccg acgcgaccaa tgggcgggcg gtggcgtcga tcttcgtcgc
      181 caagggccgg ccgcacttca atccgctcat cgtccatgtg aagaacgcag agcaagcacg
      241 cgaatatact gtgttttctt cgctagcaga agaattggcg cgggccttct ggcccggccc
      301 cctgaccctg gtgctgcccc gccgcgagga ttgccgtgtg tcgcttctgg tgagcgcggg
      361 cttggagacc atggccctgc gcgcgccctc tcacccggtc gccatgcggc tgattaccga
      421 agccggcgtc cccctggcgg cgcccagcgc caatccttcc ggacgggtga gcgcgaccct
      481 cgccgcgcat gtcgaggaag gctttcgcgg catggtggac atgatcctgg acggcgggcc
      541 caccctgata gggatcgaat caaccgtgat cggatttgag aacggcaagc ctgtgctgct
      601 gcgccccggc gccgttccac gcgaagcgct ggagcggatc accggaccgc tggcggttcc
      661 ggcccagggc gccatcatct cccccggccg tctcgccagc cactatgcgc ccaacgcgca
      721 tctgcgtctg aacgcgctgg aagcggcacc ggacgaatcc ctgctcgcct tcgggaatgt
      781 tcccggcggg gcggacgcgc ccggcggggc ggacgcgccc ggcgggcgcc gcgtgctgaa
      841 cctcagcccc agcggcgatc tgggcgaagc cgcggccaac ctgttcgcga tgctgcgccg
      901 gcttgatgcg gaaagcgatg ccatcgccgt catgcctatt ccgtccagcg gtctcggcga
      961 agccatcaac gaccggctga tgcgcgccgc agccccccga aagatcacgg catgaacgac
     1021 gatgtcgccg caaggctgaa ggctgccgcc ggtccgaagg ggttcaccga agacccggcg
     1081 gagatcgctc cgcacctgga agaatggcgc agcaagtaca agggccgcgc caccctgatg
     1141 ctcaagccgg cgacgacggc ggaagtgtcg gccatcctgg cgatctgcaa cgagagcggg
     1201 acgccagtgg tgccgcaagc cggcaacacc gggctggtcg gcgcccagat tccgttcgac
     1261 aacgaagtgc tgctcagctt gtcgcgcctg aaccgcatcc gggcgctcga tgccgaggat
     1321 gccagcatga tagcggaggc tggggtggtg ctggaggtgg cgcagaacgc ggcggacgat
     1381 aagggcctga tgctgccgct cagcctggcg gcggagggaa gctgcaccat cgggggaacg
     1441 atctcgacca acgccggcgg cgtgaacgtg ctgcgctatg gtatggcgcg cgaacaggtg
     1501 ctgggcctgg aagttgtgct ggccgacggg cgcgtgctcg acctgctgcg caccttgcgc
     1561 aaggataata cc
//
LOCUS       DASWPU010000329         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_88179,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000329 DASWPU010000000
VERSION     DASWPU010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88179"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VGC27_07050"
     CDS             <1..231
                     /locus_tag="VGC27_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0282361.1"
                     /translation="RAEHWVVVQGTAQVTCDDKVFLLHENQSTYIPLGAKHRLENPGK
                     QPLRLIEVQSGSYLGEDDIVRFEDVYGRVPAQ"
     gene            360..1274
                     /gene="cysD"
                     /locus_tag="VGC27_07055"
     CDS             360..1274
                     /gene="cysD"
                     /locus_tag="VGC27_07055"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004694369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY0282362.1"
                     /translation="MSTKTPVISHLRRLEAESIHIMREVAAEFANPVMLYSIGKDSSV
                     MLHLAMKAFYPSKPPFPLLHVDTTWKFREMIAFRDATAARLGVKLLLHVNEEGIARGI
                     NPFDSGSALHTQVMKTEALKQALDKYGFDAAFGGARRDEEKSRAKERIFSFRTATHAW
                     DPKNQRPELWRVYNTRVNKGESIRVFPLSNWTELDVWQYILAENIPIVPLYFAKKRPV
                     VERDGTLILLDDERMKLRAGEKAEDRLVRFRTLGCYPLTGAIESSAATLEAIVAEMLT
                     ARTSERQGRLIDGDEKASMEKKKREGYF"
     gene            1447..>1865
                     /locus_tag="VGC27_07060"
     CDS             1447..>1865
                     /locus_tag="VGC27_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0282363.1"
                     /translation="MSAMTPTNLKSYLAAQEKKSLLRFLTCGSVDDGKSTLIGRLLYD
                     SKLLFEDQLAALEADSKKVGTQGGDLDFALLVDGLAAEREQGITIDVAYRFFSTDKRK
                     FIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLT"
BASE COUNT          372 a          596 c          564 g          333 t
ORIGIN      
        1 cgcgccgaac actgggtcgt ggtgcagggc accgcgcagg tgacgtgtga cgacaaggtg
       61 ttcctgctgc acgagaacca gtcgacctat atcccgctcg gcgcgaaaca ccgcctggag
      121 aatccgggca agcagccgct ccgcctcatc gaagtccagt cgggctctta tctcggcgag
      181 gacgacatcg tgcggtttga ggacgtctac ggacgcgtgc cggcccagtg aggcggtctt
      241 tcggccgcaa accgagaaca gtcttgaatt gacagcccaa agatgaggat ttgaatggcg
      301 tcccttgcat aaaggagcga gcaagcctat ttcacagcgg cgtctcgggg tctgcggaat
      361 tgtccactaa aacacccgta atttctcatc tgcgccggct ggaagccgag agcatccaca
      421 tcatgcgcga ggtggcggcc gagttcgcca atccggtgat gctttattcc atcggcaagg
      481 actcgtcggt gatgctgcac ctggcgatga aggcgtttta tccctccaag ccgccgttcc
      541 cgcttctgca tgtcgatacg acttggaagt tccgcgagat gatcgcgttc cgcgacgcca
      601 ccgccgcgcg tctcggcgtc aagctgttgt tgcatgtcaa cgaggaaggc atcgcacgcg
      661 gaatcaatcc gttcgattcc ggttcggcgc tgcacactca agtcatgaaa accgaagcgc
      721 tgaagcaggc actcgacaaa tacggtttcg atgcggcctt cggcggggcg cgccgcgacg
      781 aggagaaatc ccgcgccaag gaacgcatct tttccttccg taccgcgacg cacgcctggg
      841 atcccaagaa ccagcgtccc gagctttggc gcgtctacaa cacgcgcgtc aacaagggcg
      901 aatccatccg cgtctttccc ttgtccaatt ggaccgagct ggacgtgtgg caatacatcc
      961 tggccgagaa catccccatc gtgccgctct atttcgccaa gaaacggccg gtggtcgaac
     1021 gcgacggtac gctgatcctg ctcgacgacg agcggatgaa gctgcgcgcg ggcgagaagg
     1081 cggaggatcg gctggtgcgc ttccgcaccc tgggctgcta tccgctgacc ggcgccattg
     1141 aatcctccgc cgccacgctg gaggcgatcg tcgcggaaat gctgaccgcg cgcacttcgg
     1201 agcgccaggg ccgcctgatc gacggcgacg aaaaagcctc gatggaaaag aaaaagcgcg
     1261 aggggtattt ctgatggcca ctccgtgccg tttacgcccc atatcaaccc tttcgtcatg
     1321 gccgggcttg acccggccat ccaggaaaaa ctcccccagc cgcgccgatg cgcggcggct
     1381 ggatggcgtc catacgctgc cgccatggac caagggcggc catgacgaag tggtggaggc
     1441 gtggacatga gtgcgatgac tcccaccaac ctcaaatcct atctcgccgc gcaggagaag
     1501 aagtcgctgc tgcgctttct cacctgcggc agcgtcgatg acggcaagtc cacgctgatc
     1561 ggccggctgc tctacgattc caagctcctg ttcgaggatc agctcgccgc gctcgaggcc
     1621 gacagcaaga aggtcggcac gcagggcggc gatctcgact tcgcgcttct ggtggatggg
     1681 ctcgccgccg agcgcgagca gggcatcacc atcgacgtcg cctaccgttt cttctcgacc
     1741 gacaagcgca aattcatcgt cgccgacacg ccgggccacg agcagtacac gcgcaacatg
     1801 gtcacgggcg cctcgaccgc ggaccttgcc gtgatcctga tcgacgcgcg caagggcgtc
     1861 ctcac
//
LOCUS       DASWPU010000330         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_113181,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000330 DASWPU010000000
VERSION     DASWPU010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113181"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VGC27_07065"
     CDS             complement(<1..362)
                     /locus_tag="VGC27_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY0282364.1"
                     /translation="MSAYFVTATGTDIGKTFIAAGLLRALHEAGQPVWALKPVLSGFD
                     AADARGSDPDVLLEAMGHAVSPDCIARISPWHFAAPLSPDMAAAREGKEIDFAALIAF
                     CREAVEGHRGTLLIEGVGG"
     gene            complement(359..1474)
                     /locus_tag="VGC27_07070"
     CDS             complement(359..1474)
                     /locus_tag="VGC27_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0282365.1"
                     /translation="MSSLDEFATAKLAELEDAHLRRTPAETVREDGLWVERGGRRMLS
                     FSCNDYLNLTHHPKVKAAAAAAIGKYGAGAGASRLVTGNHPLFDELEQRLARLKGTDA
                     ACVFGSGYLANAGIVPALVSEGDLLLVDELSHSCLWAGARLSRARVTMFRHNDAAHAE
                     EILSRRRASHRHALIVTDGVFSMDGDLAPLKELSTLARAHDAWLMSDDAHGLGSKHSF
                     VDLQMGTLSKALGSYGGYVCASAAAVDLVKTRARTLIYSTGLPPASAAAALAALDIVE
                     NDPELIARPLAKAQSFTRACNLPLAQSQIVPVVLGDPRTALEASQILEYEGFLVTPIR
                     PPTVPEGTARLRLAFTAGHPDAEIARLAAIVRERILA"
     gene            complement(1480..1611)
                     /locus_tag="VGC27_07075"
     CDS             complement(1480..1611)
                     /locus_tag="VGC27_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282366.1"
                     /translation="MSQVLTNPQFVMAWLDPAIQSLWARRRTPLDGRLKGGHDEVKL"
     gene            1671..2621
                     /gene="bioB"
                     /locus_tag="VGC27_07080"
     CDS             1671..2621
                     /gene="bioB"
                     /locus_tag="VGC27_07080"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005103519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY0282367.1"
                     /translation="MDDARRDWTREELAALFALPFADLMFQAQTIHRRHFTANEVQIS
                     TLLSIKTGGCPEDCGYCSQSAHHESGLKATKLMAVETVLAEARAAKDAGATRFCMGAA
                     WRSPKDKDLDAVCEMVEGVKALGLETCVTLGMLRGEQAAKLKGAGLDYYNHNIDTSPE
                     YYGQVITTRSFQDRLDTLEAVREAGINVCSGGIIGMGESEADRIGMIHALCTLPQHPE
                     SVPINLLMRVEGTKLAEAPPLDALDFVRTIAVARISMPKSFVRLSAGREYMSDEAQAL
                     CFLAGANSIFCGEKLLTTPNPEGHKDQRLLTRLGMTAMGS"
     gene            2757..4016
                     /locus_tag="VGC27_07085"
     CDS             2757..4016
                     /locus_tag="VGC27_07085"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF004624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY0282368.1"
                     /translation="MTNPDWFAKGFPHIWLPYTQMKTTPSPLAAVRTQGSRIALADGR
                     ELIDGIASWWTAVHGYNHPHIIEAVEKQARLMPHVMLGGLLHEQAATLAARLAAILPG
                     GLTRIFFSDSGSVAVEVAMKMALQYWLNRNIRRRTKFLAFKGGYHGDTIAAMAVCDPE
                     EGMHSLFKGILPEHHVIDLPRDEDSIAAFDAFLERHAGELAAILVEPLVQGAGGMIFH
                     DEYTLRRLRAACDKYELLLIFDEIFTGFGRTGAMFACEAAEVAPDIMTLVKGLSGGTL
                     PLAATAATQKIFDAFWSDDPAHALMHGPTYMGNALACAAANASLDLFERENRLRQAAD
                     MGEFLSYRLGRCLGLPHVVDVRSRGAIGVVELDRIEDLNALKRRFVDEGVWVRPFRNI
                     VYLTPALTIGEEELAKLTDAVVKVLGA"
     gene            complement(4152..>4393)
                     /locus_tag="VGC27_07090"
     CDS             complement(4152..>4393)
                     /locus_tag="VGC27_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0282369.1"
                     /translation="KGKSVSVHWELMFTRSTFQTPDMIEQHNILTRVSAMVDDGTLRT
                     TLGGHMGKIDAANLKRTHALIESGKAIGKLVLEGF"
BASE COUNT          804 a         1468 c         1377 g          744 t
ORIGIN      
        1 ccgcccacgc cctcgatcag cagcgtgccg cgatgtcctt ccaccgcttc gcggcagaat
       61 gcgatcagcg ccgcgaagtc gatttccttt ccttcccgcg ccgccgccat gtcgggcgac
      121 aagggcgcgg caaagtgcca gggcgaaatc cgcgcgatgc agtccggcga caccgcgtgg
      181 cccatcgctt ccagcagcac gtccggatcg ctgccgcgcg cgtccgccgc gtcgaagccg
      241 ctgagcacgg gcttcagcgc ccacaccggc tgacccgctt catgcagcgc gcgcagcagc
      301 ccggcggcga tgaaagtctt gccgatatcg gttccagtcg cggtaacgaa ataagcgctc
      361 atgccaggat tcgctcgcgc acaatcgcgg ccagccgcgc gatctccgcg tcgggatggc
      421 cggcggtgaa ggccaagcgc agccgcgcgg tgccttcggg caccgtcggc gggcggatcg
      481 gcgtcaccag aaagccctca tactccagga tttgcgaggc ttccagcgcc gtccgcggat
      541 cgcccagcac caccggcacg atctggcttt gcgccaaagg caggttgcag gcgcgggtga
      601 aactctgcgc cttggccagg gggcgggcga tcagttccgg gtcgttctcg acaatatcga
      661 gcgcggccaa ggcggcggca gcactggcgg gcggcaagcc ggtcgaatag atcagggtgc
      721 gtgcgcgcgt cttgacgaga tcgaccgccg ccgccgaggc gcagacgtaa ccgccgtaag
      781 accccagtgc cttcgacagc gtgcccattt gcagatcgac aaagctgtgc ttgctgccca
      841 gcccatgcgc atcgtcggac ataagccagg cgtcgtgcgc tcgcgccagc gtgctcaact
      901 ccttcagcgg cgccaagtcg ccgtccatcg agaacacccc gtcggtgacg atcagggcgt
      961 ggcgatggct ggcgcggcgc ctgcttaaaa tctcctcggc gtgagccgca tcattgtggc
     1021 ggaacatcgt cacgcgcgcc cgcgacagcc gcgcccccgc ccacaggcag gaatgcgaaa
     1081 gctcgtccac caacaagaga tcgccttcgc tcacaagcgc cggcacgatc ccggcattcg
     1141 cgagatagcc ggagccgaac acgcaggccg cgtccgttcc cttgagccgc gccagccgct
     1201 gctccaactc atcgaacaag ggatgattgc cggtcaccag ccgcgaggcg cctgcgccgg
     1261 cgccgtattt cccgatggcc gccgccgcgg cggctttgac cttcggatgg tgcgtcagat
     1321 tcaagtaatc gttgcaggag aaagacagca tccgccgccc gccgcgctcc acccaaagcc
     1381 cgtcctcgcg cacggtctcg gccggcgtgc ggcgcagatg cgcgtcctcc agttcggcaa
     1441 gcttggcggt agcgaattcg tcgagggaag acatgggcgc tatagcttta cttcgtcatg
     1501 gccgcccttg aggcggccat ccagcggcgt gcgtcggcgc gcccaaagac tctggatggc
     1561 cgggtcaagc caggccatga cgaattgggg gttggtcaaa acttggctca tcgcttgcgc
     1621 cggttccttc gtcgctataa tggcatgaac gcccataaga aggtcacccg atggacgacg
     1681 cccgccgcga ctggacgcgc gaagagcttg cggccctgtt cgccctgccc ttcgccgacc
     1741 tgatgttcca ggcgcagacc atccatcgcc ggcatttcac ggccaacgaa gtgcagattt
     1801 ccacgctttt gtcgatcaag accggcggct gcccggagga ttgcggctat tgctcgcaaa
     1861 gcgcccacca cgaatccggc ctcaaggcca ccaagctgat ggctgtcgaa accgtgctgg
     1921 cggaagcgcg cgccgcgaaa gacgcggggg ccacccgttt ctgcatgggc gcggcctggc
     1981 gctcacccaa ggacaaggac ctcgacgccg tctgcgaaat ggtggagggc gtcaaagcgc
     2041 tgggcctgga gacctgtgtc acgctcggca tgctgcgggg cgagcaggcg gcgaagctga
     2101 agggcgcggg cctcgactat tacaaccaca acatcgacac ctcgccggaa tattacggcc
     2161 aggtcatcac cacccgcagc tttcaggacc ggctcgacac gttggaggcg gtacgcgagg
     2221 cggggatcaa cgtctgtagc ggcggcatca tcggcatggg cgagagcgaa gccgaccgca
     2281 tcggcatgat ccacgcgctc tgcaccttgc cccagcatcc cgaaagcgtg ccgatcaacc
     2341 tgttgatgcg ggtggaaggc accaagctgg ccgaggcgcc gccgctcgac gcgctcgatt
     2401 tcgtgcgcac catcgcggtg gcgcgcattt cgatgcccaa atccttcgtg cggctgtcgg
     2461 cggggcgcga atatatgagc gacgaagcgc aagcgctgtg tttcctggcg ggcgccaact
     2521 cgatcttctg cggcgagaag ctgctgacca cgcccaaccc ggaggggcac aaggaccagc
     2581 gattgctgac gcggctcggc atgaccgcga tgggaagtta aatcgcaact tatttccttc
     2641 acccttcgac aagctcaggg tgagggtttg ttactaatcc ctcatgctga gcttgtcgaa
     2701 gcatgagctt gtcgaagcat gagcttgtcg aagcatgaag gggccaagtg gcacagatga
     2761 caaaccccga ctggttcgcc aaaggtttcc cgcacatctg gctgccttac acacagatga
     2821 agacgacgcc ctccccgctc gccgccgtgc gcacgcaagg cagccgcatc gcgctggccg
     2881 acgggcgcga gctgatcgac ggcatcgcct cgtggtggac ggccgtgcac ggctacaacc
     2941 atccccacat tatcgaagcc gtcgagaagc aggcgcgcct catgccgcat gtgatgctgg
     3001 gcgggctgtt gcacgaacag gcggcgacgc tggcggcgcg ccttgccgcg atcctgcccg
     3061 gcgggctgac ccgtatattc ttctcggatt ccggctcggt ggcggtggaa gtcgccatga
     3121 agatggcgct gcaatactgg ctcaaccgga atatccgtag acggacgaag ttccttgctt
     3181 tcaagggcgg ctatcacggc gacaccatcg ccgccatggc ggtgtgcgat cccgaagagg
     3241 gcatgcattc gctgttcaag ggcatcctgc ccgaacatca cgtcatcgat ctgccgcgcg
     3301 acgaagacag catcgccgcc ttcgacgcct tcctcgaacg ccatgcgggt gaactcgccg
     3361 ccatcctggt cgagcccttg gtgcaaggcg cgggcggcat gatcttccac gacgagtaca
     3421 ccctgcgccg cctgcgcgcg gcgtgcgaca aatacgagct gctgctgatc ttcgacgaaa
     3481 tcttcaccgg tttcgggcgc accggggcga tgttcgcctg cgaagcggcc gaggtcgcgc
     3541 ccgatatcat gaccctcgtc aagggtctca gcggcggaac gctgccgctg gccgccaccg
     3601 ccgccacgca aaagatattt gacgctttct ggtccgacga tcccgcccac gccctgatgc
     3661 acggcccgac ttatatgggc aacgcgctgg cttgcgccgc cgccaacgcc tcgctcgacc
     3721 tgttcgagcg cgaaaaccgg ctgcgccagg ccgccgacat gggcgaattc ctcagctata
     3781 ggctcggccg atgcctgggc ttgcctcatg tcgtggacgt gcgttcgcgc ggcgccatcg
     3841 gcgtggtgga actcgaccgc atcgaagacc tgaatgcgct caaacgccgc ttcgtggacg
     3901 aaggcgtctg ggtccgcccc ttccgcaaca tcgtgtacct cacgcccgcg ctgaccatcg
     3961 gcgaagagga attggcaaag ctgacggacg ccgtagtgaa agtgctgggc gcctaaggcg
     4021 gtttcaagtt aacgtcacaa aaaaattcgt catggccgcc cctgaggcgg ccatccagcc
     4081 tgtcccgccg aagcgcttac gcgaaggcgg caagcccggc catgacgaag tgatgggtgt
     4141 ggcgctcgtc ctcaaaaccc ttccagaacc agcttgccta tcgccttgcc gctttcgatc
     4201 agcgcatgcg tccgcttgag gttcgccgcg tcgatcttgc ccatatggcc gcccaaagtg
     4261 gtgcgcaggg tgccgtcgtc aaccatggcg ctgacccgcg tcaggatatt gtgctgctcg
     4321 atcatgtccg gggtctggaa ggtggagcgc gtgaacatca gttcccaatg caccgagacg
     4381 ctcttgccct tga
//
LOCUS       DASWPU010000331         4210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_14994,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000331 DASWPU010000000
VERSION     DASWPU010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14994"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VGC27_07095"
     CDS             complement(<1..499)
                     /locus_tag="VGC27_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY0282370.1"
                     /translation="MSGAGRRTIGAYQPRPRGAAMADALDDARHALARLSRLLVMRGA
                     GLVVLLATLAVLIALVSYDSGDASLNNATGGEVSNLLGPIGATAADLLLQLFGYAALA
                     FAIPLTVWGTRALMGRTLRHALARAFAWLLGTIFLAAGLGILPPPESLPAGAGGMIGI
                     AVAALS"
     gene            complement(496..1680)
                     /locus_tag="VGC27_07100"
     CDS             complement(496..1680)
                     /locus_tag="VGC27_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0282371.1"
                     /translation="MLPGRLAALPPSAFAKLATLLDDIPPGRPPVSLAVGDPQGEVPP
                     FVMEAIARHADRFGFYPPIDGTAEWREAATGWLKRRFALPGAPAVLPLNGTREGLFLA
                     PFIVTPEKKAGARPVILLPNPFYQCYAAAALSCGAEPVFVRADAAGGFLPDFAALPKT
                     VLERVVAAYLCSPSNPEGACASESYWRTLFALADEYDFTLFADECYADIYRGKPPVGA
                     LSVRGAPFERLLSFHSLSKRSGLPGLRSGMVAGDAGLMAKFRALRNYAGPQVPLPLVA
                     ASAAAWSDEIHVAANRARYAERFDLAERLLGTRWSFRLPEGGFFLWLEVGDGAQTALR
                     LWREGGVRVLPGAYMGQESKRGNPRTNPGFPYVRVALVNDLSTIMKALERMVEILGRE
                     TA"
     gene            complement(2090..3412)
                     /locus_tag="VGC27_07105"
     CDS             complement(2090..3412)
                     /locus_tag="VGC27_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY0282372.1"
                     /translation="MKILLSKRPVLAALASVGTLIATASSAFAATEKLDSGDTAWMLT
                     SSVLVLLMTIPGLALFYGGMVRRKNVLATMAQSFGTTCLITVVWMVVGYSIVFSPNPD
                     ESWNQVLGGFSYLFLKPLTIDAMSGTIPESVFVFFQMTFAIITPALIAGALADRMKYS
                     AFLWFMGLWVLLVYCPIAHWVWGGGWLMTAGVLDFAGGSVVHLNAGTAALVGCLVLGK
                     RAGYGTENMAPHNLVFSIIGASLLWVGWFGFNAGSAAAANANAGMAAAATQIATAAAS
                     LTWMFSEWLIAKKPSVLGMISGAIAGLVAITPAAGFVDPVGALIIGLAAGVVCYIASV
                     WVKKLLGYDDSLDAFGVHGVGGALGALLTGVFASNAINSGAHGWLIDGNFGQMWLQVE
                     GVVATFVYCGIGTFIILKVIDMIIGLRVSHEVEVEGLDINLHGETVHG"
     gene            complement(3452..3790)
                     /locus_tag="VGC27_07110"
     CDS             complement(3452..3790)
                     /locus_tag="VGC27_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY0282373.1"
                     /translation="MKLIMAIIKPFKLDEVRESLSALGVQGMTVTEVKGYGRQKGHTE
                     IYRGAEYAVSFLPKLKIEVAVKAEMTEAVIEAITSKAKTGQIGDGKIFVTQLAQVVRI
                     RTGETDGDAL"
BASE COUNT          833 a         1326 c         1359 g          692 t
ORIGIN      
        1 gcgacagggc cgcgaccgcg atcccgatca tgccgccggc gccggccggc aggctttcgg
       61 gcggcggcag gattcccagg ccggcggcga gaaagatggt gccgagcagc caggcgaagg
      121 cgcgcgccag ggcgtggcgc agcgtccggc ccatcagggc gcgcgtgccc cagaccgtca
      181 gcggaatcgc gaaggccagc gccgcatagc cgaacagttg cagcagcaga tcggccgcgg
      241 tggcgccgat cgggccgagc aggttggaaa cctcgccccc ggtggcattg ttcaggctgg
      301 cgtcgccgga atcgtagctg accagcgcaa tcagcaccgc gagggtggcc agcagcacga
      361 cgaggcccgc accccgcatg acgaggaggc gcgacagccg ggcgagcgca tggcgggcgt
      421 cgtccaaggc atcggccatg gccgccccgc gcggacgcgg ctgataggct ccgatcgtgc
      481 ggcgtccggc tccgctcatg ccgtttccct gcccaggatt tcgaccatcc tttcgagtgc
      541 cttcataatg gttgataaat cgttgacgag cgcgacgcgg acatagggaa atccagggtt
      601 tgtgcgggga tttccccgct tactttcttg acccatatag gcgccgggca agacgcgcac
      661 cccaccctcg cgccatagcc taagcgccgt ttgggcgccg tcgccgacct ccagccatag
      721 aaaaaagccc ccttccggca agcgaaagct ccaacgggtg cccaacaggc gctcggccag
      781 gtcgaaacgc tccgcataac gcgcccggtt cgccgccaca tggatttcgt cgctccaggc
      841 cgccgccgag gccgcgacca gcggcaaggg cacctgcggt ccggcataat tgcgcaaggc
      901 gcggaacttc gccatcagcc ctgcgtcgcc ggcgaccatg ccggagcgca atccgggcaa
      961 gcccgaacgt ttcgacaagg agtggaaact gagcagccgc tcgaacggcg cgccgcgcac
     1021 gctgagcgct ccgacgggcg gtttgccgcg atagatatcg gcgtagcatt cgtctgcgaa
     1081 cagtgtgaag tcgtattcgt cggccagcgc gaacagcgtt cgccaatagc tttcgctggc
     1141 gcaggcccct tcgggattcg acggcgagca caaataggcg gcaacgactc gttccaggac
     1201 cgtcttgggc agagccgcga aatcgggaag gaatccaccc gccgcgtcgg cgcgcacgaa
     1261 caccggttcc gccccgcacg acaaagccgc ggcggcgtag cattggtaga aggggttagg
     1321 cagcaaaatg acgggccgtg cgcccgcctt tttctccggc gtgacgatga agggcgccag
     1381 gaacagcccc tcgcgggtgc cgttgagcgg cagcacggcg ggagcgcctg gcaaggcgaa
     1441 gcgccgcttc agccagcccg ttgccgcctc gcgccactcg gcagtgccgt cgatcggagg
     1501 atagaaaccg aacctgtccg catggcgcgc gatcgcctcc atcacgaagg gcggcacttc
     1561 gccctgcgga tcgccgaccg ccaggctgac gggcggtcgg cccggcggga tgtcgtccag
     1621 cagcgtcgcg agcttggcaa aggcagaagg cggaagcgcg gcaaggcggc ctggtaacac
     1681 gatctgtccc cgcgattgac attcgatggt aggcgcgccg gcctcgagcg tccatcgcca
     1741 taaatacgcc gtatggcgac tttttatgcg ccgccctgat tggagatcaa agtgaaattg
     1801 ccgccgcttg tatttacggc cgccgcgctg cttctgcttg gcgcttcgtc ttccgcggcg
     1861 gaccaggccc cgctcaaaag gggattcgga aatctgagtt ttcggcacgc acggcccgat
     1921 gccggacgga cagaaactgg aggattcccc gccgccgctg cgcagcaccg gcgtggccgg
     1981 gaagctgcgc gcccagggcc gtcgttattg aaggtcccgc cgaataaaga aaaggggcgc
     2041 tccttgcgga gcgccccagt ttcgaggagg aaggcggtat acggagggat cagccgtgta
     2101 ccgtttcgcc atggaggttg atgtcgagac cctcgacttc gacctcgtgg gagacacgca
     2161 gaccgatgat catgtcgatc actttcagga tgatgaaggt tccgatgccg cagtagacga
     2221 aggtggcgac gacgccttcg acctgcagcc acatttgtcc gaaattgccg tcgatcagcc
     2281 agccgtgtgc gccggagttg atggcattgg aggcaaacac gccggtcagc agggcgccga
     2341 gtgccccgcc cacgccgtgc acgccgaacg cgtcgagcga gtcgtcatag ccgagcagct
     2401 tcttcaccca caccgacgcg atgtagcaga cgacgcccgc ggccaagccg atgatcaacg
     2461 cgccgaccgg atcgacgaag cccgcagccg gagtgatcgc caccagtccg gcaatcgcgc
     2521 cggagatcat gccgagcacc gaaggcttct tggcgatgag ccattccgag aacatccagg
     2581 tgagcgaggc ggcggccgtg gcgatctggg tcgcggcggc ggccatgccg gcattggcgt
     2641 tggcggccgc ggcggagccg gcgttgaagc cgaaccagcc gacccacaac agcgaggcgc
     2701 cgatgatgct aaacaccaga ttgtgcggtg ccatgttttc ggtgccgtac ccggcgcgct
     2761 tgccgagcac caggcagccg acgagagccg cggtaccggc gttcagatgc acgaccgaac
     2821 cgccggcgaa atcgagcacg cctgccgtca tcagccagcc gccgccccag acccagtggg
     2881 cgatcggaca atagacgagc agcacccaca gccccatgaa ccacagaaag gccgaatact
     2941 tcatgcggtc ggccaaggcg ccggcgatca gcgccggagt gatgatcgca aaggtcatct
     3001 ggaagaacac gaagacggat tcgggaatgg tgcccgacat cgcgtcgatg gtcagcggct
     3061 tcaggaacag ataggagaag ccgccgagaa cctggttcca gctctcgtcc ggattcggcg
     3121 agaagactat cgagtaaccg acaaccatcc acaccacggt gatgaggcag gtcgttccga
     3181 agctctgcgc catcgtggcc agaacgttct tccggcgcac catgccgcca tagaacaggg
     3241 ccaggcccgg aatggtcatc aagagcacga gaacgctgga ggtgagcatc caggcggtgt
     3301 cgcccgagtc gagtttctcg gtggcggcga aggcgctgct cgcggtcgcg atcaaggttc
     3361 cgacacttgc cagcgcagcg agcaccggcc ttttcgaaag caagattttc attctaattc
     3421 cccttagttg atcccaagtg cgagtcgcgt gtcacaacgc gtcgccgtct gtttcgccgg
     3481 tacgaatgcg aacgacctgc gcgagctgcg taacaaagat cttgccgtcg ccgatctggc
     3541 cggtcttggc cttggaggtg atcgcctcga tcaccgcttc ggtcatttcg gccttgacgg
     3601 ccacttcgat cttgagtttg ggcaggaagc tgactgcgta ctccgcgccg cggtagattt
     3661 ccgtatgccc cttctgacgc ccgtaccctt tgacttcggt gaccgtcatg ccttggacgc
     3721 caagtgcgga gagcgactcg cgcacctcgt cgagcttgaa cggtttgatg atcgccataa
     3781 taagtttcat cgccggtctt ccatgtgtgg cctcttaaca cttcatcaag aaccgtgccg
     3841 cacctgccgg aatgtataaa cctttgtaat tgatggaatt tttctagaaa cagcaggaca
     3901 agaagcggaa atggaaggtg cgtatgcaca aatattgtgc gttatttgaa taacgcctac
     3961 gctttaggca ttgaccagag gcattctgca tggtccttcg agatgccctt cgccctcatc
     4021 ttgagaaggc gcgaagcgcc gtctcgaagg acgggcgaaa gggcacctca ggatgaggcg
     4081 aaacagctat gacgaagacg gtcgatgtga tcatcgctgg cggcgggatg gtgggcctaa
     4141 cgctgggcat cgctctggcg aagggcgggc ttgaggtcgc cgccgccgat ccggtgcctg
     4201 aaaccgatgc
//
LOCUS       DASWPU010000332         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_2523, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000332 DASWPU010000000
VERSION     DASWPU010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2523"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..1035)
                     /locus_tag="VGC27_07115"
     CDS             complement(343..1035)
                     /locus_tag="VGC27_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282374.1"
                     /translation="MGMKSKLMVGGCVLALCLAAAGGAQAQKALKCANPAEVTAMQAT
                     AVQQELMDAALTCGESAMTNFNAFQTSFGPELRRSDKTLLRMFQRVLGYKKGDAAYNL
                     YKTELASKAELRRLKGHQDFCTAANLVISAALVPERPSLNDFVSGVQVKDIEGPVQGC
                     NVEVAVTLQGAMAGPDILPKPNPLRLAALTPPAPVAAPEPAVAPSAPAPEPVQEQPKE
                     KKKSGWFSGLWN"
     gene            complement(1104..1358)
                     /locus_tag="VGC27_07120"
     CDS             complement(1104..1358)
                     /locus_tag="VGC27_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282375.1"
                     /translation="MVGSPSPAGARVLENRSKSHRVAGQFLLRPAPVAAARLLGGSGG
                     GKRHNAAIRIVRMQAGLGTEWMFNLNASLKKALKSFLTMR"
     gene            1515..2537
                     /locus_tag="VGC27_07125"
     CDS             1515..2537
                     /locus_tag="VGC27_07125"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY0282376.1"
                     /translation="MSYKVAVVGATGNVGREMLSVLAERQFPVSEVVALASTKSIGTD
                     VSFGDKILKVKALDYYDFKGTDVVLMSAGGKVSKEWSPKIAAAGAIVIDNSSTWRMDR
                     EVPLVVPEVNAAALDGIKKGIIANPNCSTAQLVVALKPLHDEAKITRVVVATYQSVSG
                     AGKEAMDELFRQTRAVFVTDPIETHKFTKQIAFNVIPHIDVFMDDGYTKEEWKMMVEV
                     QKILDPDIQVNATCVRVPVFIGHSEVANIEFERPITAQRARAILREAPGCLVVDKHED
                     GGYITPVEAAGEDATYISRIRKDPTVEHGLTLWCVSDNLRKGAALNAVQIAECLINRK
                     LLKTAA"
BASE COUNT          522 a          902 c          872 g          483 t
ORIGIN      
        1 accaaagctc tagagcatcg gacgctcttg cgcaagcaag gcgccaggac catcgaggcc
       61 ttgtggcagg cgatcggcaa catctgcgat ctcttcacat tcagcgaatg ctggaatttc
      121 tttcggcacg atggttatgc gcccgacttc acatgagcgt ccgatgctct agagctttgg
      181 tccgccgtag cgcagcgaag gcgggcaagc tcatgcttcg acagtcggta gcaacagcgt
      241 gccgaccagc atgagggatt ggaaaatgcc ctcaccctga gcctgtcgaa gggtggggcg
      301 attccgtctg aatgcatttt gctctagcgc ccggcggcgg cgtcagttcc agaggcccga
      361 aaaccacccg gatttctttt tttccttggg ctgttcctga accggctcag gcgccggggc
      421 ggaaggagcg acagccggtt ccggcgcggc gacgggcgcg gggggcgtca gcgcggccag
      481 ccgcagcggg ttcggcttgg gcaagatgtc cgggcccgcc atcgcgcctt gcagcgtcac
      541 cgccacttcc acattgcagc cctggaccgg accttcgata tccttgacct gaacgccgct
      601 tacaaagtcg ttcaggctgg gccgttcggg caccagtgcc gcggagatca ccagattggc
      661 cgcggtgcag aaatcctgat gccccttcag gcggcgcagc tcggctttag acgccagctc
      721 cgtcttgtac aaattgtagg ccgcatcgcc cttcttgtag cccaaaacgc gttggaacat
      781 ccgaagcagc gttttgtcgg agcggcgcaa ctcgggcccg aaactggtct ggaaggcgtt
      841 gaaattggtc atggccgact cgccgcaagt cagggccgcg tccatcagct cctgctggac
      901 ggccgtggct tgcatggctg taacctccgc cggattggcg catttcagcg ccttttgggc
      961 ctgcgccccg ccggccgcag ccaggcaaag cgccagtacg cagccaccga ccatcagttt
     1021 gcttttcata cccatgaacc ccgacccctt tcggcttttg ccgggaccat agcaccaccg
     1081 ccgcgcgtcc cgcgagtctt tcactaccgc attgttaaga acgatttcag cgccttcttc
     1141 aatgaagcat tcagattaaa catccattct gtaccaaggc cagcctgcat gcgcacgatt
     1201 ctgatcgccg cgttgtgtcg tttgccgccg ccgcttccgc cgaggagccg ggcggccgcg
     1261 acgggcgctg ggcgcaggag gaactgcccg gcgacgcgat gggacttgga cctgttctcc
     1321 aggaccctgg cgccggcggg cgaaggcgaa ccgaccatcg ccggcaccgt ccgcaaaaac
     1381 gcggccgcaa ttccgctgcg ccggcacccg ggcgaatagc agcaccgccg cccgcgccct
     1441 ttcctgtctc agcccgttct agtatttgtg cccgccccgg cgttccggcc ggattggcta
     1501 aggatcgaag tgtcatgtct tataaagtcg ccgtcgtcgg cgcgaccggc aatgtcgggc
     1561 gcgagatgct gagcgtgctg gccgagcgcc agttccccgt cagcgaagtc gtggcgctgg
     1621 cctcgaccaa atccatcggc acggacgtgt cgttcggcga caagattctg aaggtcaaag
     1681 cgctcgacta ttacgacttc aagggcaccg acgtggtgct gatgagcgcg ggcggcaagg
     1741 tctccaagga atggtcgccg aaaatcgccg cggccggcgc catcgtcatc gacaattcct
     1801 cgacctggcg catggaccgc gaagtgccgc tggtggtgcc ggaagtcaac gccgccgcgc
     1861 tcgacggcat caagaagggc atcatcgcca atcccaactg ctcgacggcg cagctcgtgg
     1921 tggcgctcaa gccgctgcac gacgaagcca agatcacgcg cgtggtggtg gccacctatc
     1981 aatcggtttc cggcgccggc aaggaggcga tggacgaatt gttccgccag acccgcgcgg
     2041 tgttcgtcac cgacccgatc gagacgcaca aattcaccaa gcagatcgcc ttcaacgtca
     2101 tcccgcacat cgacgtgttc atggacgacg gctacaccaa ggaagagtgg aagatgatgg
     2161 tcgaggtgca gaagatcctc gatcccgaca tccaggtgaa cgccacttgc gtgcgcgtgc
     2221 cggtgttcat cggccattcc gaagtggcga atatcgagtt cgagcggccg atcaccgcgc
     2281 agcgggcgcg cgccatcctg cgcgaggcgc cgggctgtct tgtcgtcgac aagcacgagg
     2341 acggcggcta catcacgccg gtggaagccg ccggcgaaga cgccacctat atcagccgca
     2401 tccgcaagga cccgacggtg gagcatgggc taacgctgtg gtgcgtctcg gacaatctgc
     2461 gcaagggcgc ggcgctcaac gcggtgcaga tcgccgagtg cctgatcaac cgcaagctgc
     2521 tcaagaccgc cgcatgacac gggagcgcga ccaggcgaag tgtgaagcgg ttcgccgttc
     2581 gggcgcgcga ccctcaaaaa gcaggacgtg atgacgcagg aatcgaaatc ggcggcagcg
     2641 atccgtccgc gccgcagcgt gctttacatg cccggctcca acgcgcgcgc gctcgaaaag
     2701 gcgcgcacgc tgccggcgga tgcgctgatc ctcgacctgg aggacgcggt cgcccccgac
     2761 gccaaggagg cggcccgcg
//
LOCUS       DASWPU010000333         6750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_106960,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000333 DASWPU010000000
VERSION     DASWPU010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106960"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1912)
                     /locus_tag="VGC27_07130"
     CDS             complement(<1..1912)
                     /locus_tag="VGC27_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEY0282377.1"
                     /translation="MPDRRELKGLNVAKISIRSYPPMIANDRAACCPLTFQQERVLYF
                     CELNPNSSIWDINTCKRLTGHMDPRRLAKAVERLAAWHQALRTRIVRREDGPAQFFDN
                     DTARAFRHIDISTAARRGADEALAAKIAEICRKPISTPTYEEVLFEVVLLTLGETDSA
                     LLLRLHHIISDAASVDILWRDLARLYNGLARGTPETPPERIQYSDYAFWQRHHLGPDQ
                     TREQEEYWLGKFRDLPPPLDLPADAAPSPGLSFNGGLEIVSLPKDLVDDFQKLSWDRR
                     VLPFSSLFAAYLVLLYKLCQQEDMAVGLLFSGRHYRPDLKDVVGFFVNLTAARVAVRP
                     DCTFDQLVSAVHEEVEAAYFMQDYPFERLVQQLAPKRNDARMPLLHTMFNVVSNTEDD
                     ESLDGIETMQTIDVATQTNAVQVDLIFDIHLGSKDAEIRIEHNSDIFYRETVRRFADH
                     FVTLLRALRSDWNIPLSRLELIDEAEKNRLLTGGARQSGHCVHEVFVQQVRRNPDKIA
                     LVDSGRKLTYAQLDQVSSRLADLLARHGVGPEKIVAVIGGRSADMVIGQLAILKAGGA
                     YLPIAHSIPRARIREVLDDASPCVVLLADNCGHDTGFAGPQLRVTDGTNGAEHDHVRI
                     RRVKPSNLAYVIY"
     gene            complement(1891..5133)
                     /locus_tag="VGC27_07135"
     CDS             complement(1891..5133)
                     /locus_tag="VGC27_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0282378.1"
                     /translation="MSTSPASSPAIEHCDGDVRAYPLAFQQERVLYLNKLSPQGALWN
                     RVSCKHLTGEIDVTALTLAIDDLVERHSVLRTRIALADGEPFQSKHDRIADPFEYIDA
                     TAYGSDAQAKEILNRLYETPFSLEDGVLFKAFLVRRGERKFWLILKLHHIISDATTLQ
                     ILWDDINALYNFRLGQGEPLAAPEIEYFDYAVWLRRRFRDDGLKAQEDYWLQTFSGAL
                     PELDLPADTGAPASLTFTAAAASAKLPLPLVSRLKSFSLSERVIAFSTMLSAYYLLLH
                     HYCRQDDIVIGTVFSGRHHSPQIRRLAGFFTNTVALRLHLDAEQSVRQFVAATHALVS
                     RAYKMQDYPFEWLVEKLGPDRAQRRNPLFRAMFNVLNVPAARTAMHGVQHEEWLEPEI
                     SASQVDLFLEIRLGQEPEVRIEYNAEIFTRPLILRMLRHYIALLGGIVESAEANACDL
                     SMLDASETALIQSFGDGGSGAPARARTVVDLLEERALRSPDLPALLHAGGAITCREVN
                     RLANRLAATLASSGVQRGDIVAVMIDRSPQMVIALLATLKAGAAYLPIDPQCPPQRIA
                     YILNDSHARCLLTGRATIQATPAHLGSVHCIAVDDDASYRGDGENSGGVPTGADAAYV
                     IYTSGSTGTPKGVVVEHGALMNTLDCLNARYPVDGKTILLKTNYTFDVSAAELFGWLF
                     ESGRLAVLDKDAEKEPARIREAIERYGVTHINFVPSMLDALLSGAQECDLRTLERLQY
                     VFVAGEALKPELVSLFHKRVRNVRLENLYGPTEAAIYATGYSMPRDGVLSHVPIGKPL
                     PNTRAYILDDKLRVVPIGVSGELCLSGAGLARGYLNQPVLTGDRFRPNPHWPGQTLYK
                     TGDLTRWNEDGLIQFLGRFDGQVKIRGFRVEIGEVERKLRSCRGVAEAAVAAKADPFG
                     QPELVAYAVMQAGQELRCDDLRKELTAWLPGYMIPSWFVPLERLPRLASGKIDGKALP
                     EPRRQSEPAAAAATELEKTIIAIAERLLNARGLDPSSSFFRLGGNSLLTLRFIAALDD
                     ALGMRLSVMDFLELPTIRDIAKHIEELRSNPTATVPTTSCRTRECRIGVN"
     gene            complement(5159..6334)
                     /locus_tag="VGC27_07140"
     CDS             complement(5159..6334)
                     /locus_tag="VGC27_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0282379.1"
                     /translation="MIGADSNILKSGAAFARKVIAPVAASADETETFPADIFREIGCF
                     GIFGTPYPEQYEGSGLNFATYLDLIGEIARYSAAVAMSTVAHATLACEPIWSAGSEGL
                     KARYLPALLSGVKIGAFAMTESGSGSDISALTTCAEDDGDGFILNGSKLFITNANIAD
                     IIVVVAKTSRRAGVMGLSLLLVEKDMPGVLTTGKSEKKLGMRASDTGELVFHDVRIPK
                     TNLIGRKDRGFEVLQRTLSAARLGMAAIAIGLAKAARDLSLDYAVQRRQFGQPLYRFQ
                     LVKRMLADMEMKIDAAELLLANGARLRDSGQGYAKQASEAKLFASETAMEVTRDAIQI
                     HGAYGYCRDLPLERLFRDAKLTEIGDGTSEIQRLIIADEMMKERANRLAGLAPEDGQ"
     gene            complement(6331..>6750)
                     /locus_tag="VGC27_07145"
     CDS             complement(6331..>6750)
                     /locus_tag="VGC27_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY0282380.1"
                     /translation="RLDKTSMAWGVESRVPFLDHRLVEYVVHMPSIYKSSPDCEKILL
                     KAIAAELLPADVIARKKRPVPFPVDPLTVLHERKYANDLVQAPGSRIGNYFDKRKTAD
                     FLNKRGQFATIDDLAVYRTSHALIALDMWHQAFGVAA"
BASE COUNT         1206 a         2033 c         2184 g         1327 t
ORIGIN      
        1 tgtagatgac gtaggccagg ttcgacggct tgacgcgccg gatccggacg tgatcgtgtt
       61 cggcgccatt cgtgccgtcc gtgacgcgca gctgcggtcc tgcgaacccc gtgtcgtgac
      121 cacaattgtc cgctagcaag acaacgcagg gcgaagcatc gtcgagtact tcccggattc
      181 tcgcccgcgg gatggagtgc gcgatcggca gataggcgcc gcccgccttc aatatggcga
      241 gttgccctat caccatatca gccgaacggc cgccgatcac ggcaacaatc ttctcgggtc
      301 cgacgccatg tctggcgagg agatcggcta gacggctcga tacttggtcg agctgcgcat
      361 aggtgagttt cctgccgcta tcgaccagcg cgatcttgtc ggggttgcgg cgtacctgtt
      421 gcacgaacac ttcgtgcaca cagtggccgc tctggcgagc gccgccggtg aggaggcgat
      481 tcttttccgc ttcatcaatc aattcaagtc tgctgagcgg aatgttccaa tccgaacgca
      541 gggcgcggag aagcgtgacg aaatggtctg caaaccgcct gacagtttcc ctgtagaaga
      601 tatccgagtt gtgttcgatc cggatctccg cgtctttcga accgagatga atgtcgaata
      661 tcagatcgac ttggaccgcg ttggtctgcg ttgcgacgtc tatggtctgc attgtctcga
      721 tgccgtccaa cgattcgtcg tcctccgtgt tcgagacgac gttgaacatg gtatgcagca
      781 gcggcatgcg cgcgtcgtta cgcttcggcg caagctgctg cacgagcctc tcgaacggat
      841 agtcctgcat gaaataagcg gcctcgactt cctcgtgcac cgcggatacg agttgatcga
      901 aggtgcaatc cggccggaca gcaacgcgcg cggcggtcag gttcacgaag aaaccgacga
      961 cgtccttgag gtccggccga tagtgccggc cggagaacag tagtccgacc gccatgtcct
     1021 cctgctggca gagcttgtac agaagcacga ggtaggcggc gaaaaggctc gagaacggca
     1081 gcacccttct gtcccagctc agtttctgga agtcgtccac caggtcctta gggagggaga
     1141 cgatctcaag cccgccattg aaggacaggc cgggagaggg ggccgcgtcg gcgggcaggt
     1201 ccagcggtgg aggcagatcg cggaatttgc cgagccagta ttcttcctgt tcgcgcgtct
     1261 ggtccggtcc gagatggtgc ctctgccaga aggcgtagtc ggagtactgg attcgctctg
     1321 gtggcgtttc gggcgttccg cgagcgaggc cgttatagag tcttgccaga tcgcgccaca
     1381 ggatatccac cgatgccgcg tcggagatga tgtgatggag gcgcaggagc agcgcgctgt
     1441 ccgtctcgcc aagcgtgagc agcaccacct cgaacagaac ctcctcatag gtcggcgtgg
     1501 agatcggttt gcggcaaatt tcggcgatct tcgcggcaag agcctcgtcg gcgccgcggc
     1561 gcgctgcggt cgagatgtcg atgtggcgga aggcgcgggc ggtgtcgttg tcgaagaact
     1621 gtgcggggcc gtcttcgcgg cgcacgattc tggtccgcaa cgcctgatgc caggctgcca
     1681 gccgctcaac cgccttcgcg aggcggcggg gatccatgtg gccggtgagc cgcttgcagg
     1741 tgttgatgtc ccagatcgag ctgttgggat tgagttcgca gaaatagagg actctctcct
     1801 gctggaacgt cagtggacag cacgccgcgc ggtcgttcgc gatcatcggc ggataactgc
     1861 ggatgctgat cttcgccacg ttcaggccct tcagttcacg ccgatccggc attcgcgggt
     1921 gcggcagctc gttgtcggga ccgtagcagt cgggttgctc cgaagttcct cgatgtgctt
     1981 ggcgatatcg cggatcgtcg gaagttccag gaagtccatg accgaaagcc gcatgcccaa
     2041 ggcgtcgtcc agcgctgcaa tgaagcgcag cgtcagaagc gaattgccac ccagccggaa
     2101 gaagctgctg gaaggatcaa ggccgcgcgc gttgagtagc cgctccgcga tggcgatgat
     2161 cgtcttctcc agttccgttg ccgctgccgc cgcaggctcg ctctgccgac gcggctcggg
     2221 cagcgccttg ccgtcgatct tgccgcttgc caggcgggga agccgctcca gcggcacgaa
     2281 ccaggacggg atcatgtaac cgggcagcca cgcggtcagc tctttgcgga gatcatcgca
     2341 ccgcaactcc tggccggctt gcatcacggc ataggcgacg agctccggct gcccgaaggg
     2401 gtctgccttg gccgcaacgg cggcttccgc cacgccgcgg cagctgcgca gcttgcgttc
     2461 aacctcgccg atctcgacgc ggaagccgcg gatcttcacc tgtccgtcga aacggccgag
     2521 gaactggata agcccgtcct cgttccagcg ggtaaggtcg cccgttttgt agagcgtttg
     2581 gcccggccag tggggattgg gacggaaacg gtccccggtc aggacgggct ggttgagata
     2641 gccgcgcgcc aagccggcgc cggaaaggca gagttcgccg ctgacgccga tcggcaccac
     2701 ccgaagcttg tcgtcgagga tgtaagctct ggtgttgggc aacggcttgc cgatgggaac
     2761 gtgcgataaa acgccgtcgc gcggcatgga atagccggtt gcatagatgg ccgcctcagt
     2821 cggtccgtag aggttttcga ggcgcacgtt gcgaacccgc ttgtgaaaga ggctcacgag
     2881 ctccggcttc agcgcctcgc cggcgacgaa tacgtactgc aggcgttcga gcgtccgcag
     2941 atcgcattcc tgcgcgcccg acagcagcgc gtcgagcatg gagggaacga aattgatgtg
     3001 ggttacgccg tagcgctcta tggcttcccg gatcctggcg ggttccttct ccgcatcctt
     3061 gtcgagcacg gccaggcggc cgctctcgaa cagccagccg aaaagctccg cagccgagac
     3121 gtcgaaggta tagtttgtct tgagcaggat agtcttgccg tccacgggat agcgcgcgtt
     3181 gagacaatcc agtgtgttca tcagggcgcc gtgctcgacg accacgccct ttggcgtacc
     3241 ggtggatccg gacgtgtaga tgacgtacgc cgcgtccgcg cccgtcggga cgccgccgga
     3301 gttctcgccg tcacccctgt aggatgcgtc gtcgtccacc gcgatgcagt gaaccgagcc
     3361 gagatgggca ggtgtcgcct gtattgtcgc gcgtccggtc aacaggcaac gggcatggct
     3421 gtcgttcagg atgtaggcga ttctttgcgg tgggcattgc ggatcgatcg gcagataggc
     3481 cgcacccgcc ttgagcgtcg ccagcaaggc gatcaccatc tgcggtgagc ggtcgatcat
     3541 gaccgcgacg atgtcgccgc gttgcacgcc ggacgaggcc agcgtggccg caagcctgtt
     3601 tgccagccga ttcacctcac gacaggtgat cgcgccgccc gcgtgcagga gcgccggcag
     3661 gtctggagag cgcaacgccc gttcctcaag caggtccacg accgtgcgcg cgcgcgccgg
     3721 cgcgcctgag ccgccgtcac cgaaggattg gatgagggcc gtctcggacg catcgagcat
     3781 gctgagatcg caggcgttcg cctcggcgga ctcgacgatg ccgccgagca gcgcaatata
     3841 gtggcgcagc attcgcagta tcagcggcct ggtaaatatc tcggcgttgt attcgatgcg
     3901 gacttccggt tcctggccga ggcggatttc gagaaacaga tcgacctggc tggcgctgat
     3961 ctccggctcc agccattctt cgtgctgcac gccgtgcatc gctgtgcgtg ccgccggaac
     4021 gttcagcacg ttgaacatcg cgcgaaagag cggattgcgc ctttgggcgc ggtccggccc
     4081 cagcttctcg accagccact cgaatgggta atcctgcatt ttgtaagccc ggctgaccag
     4141 agcatgcgtc gcggccacga actgcctgac ggattgctcc gcatccagat gcaggcgcag
     4201 cgcaactgtg ttggtgaaga aaccggccag ccgcctgatt tgagggctat ggtggcggcc
     4261 agagaacacc gttccgatga cgatgtcgtc ctgccggcaa tagtggtgca gcagaaggta
     4321 ataggcactc agcatcgtgg aaaacgcgat cacgcgttcc gacaggctga aagacttgag
     4381 gcggctcacc aggggcagcg gcagtttggc gctcgccgcc gcggcggtga aggtcaggct
     4441 ggccggtgcg ccggtatccg ccggcagatc aagctccggc aaagcgcctg agaaggtctg
     4501 cagccaatag tcttcctgcg ccttgagtcc atcgtcgcgg aaccggcggc gaagccatac
     4561 ggcgtagtcg aaatattcga tctccggcgc tgccaggggt tcgccctggc cgagccggaa
     4621 attgtacaaa gcgttgatat cgtcccagag gatctgcagc gtcgtcgcgt cggagatgat
     4681 gtgatgaagc ttcaggatca gccagaattt ccgctcgccc cgccgcacca gaaaggcttt
     4741 gaacagcacg ccgtcttcga ggctgaacgg cgtctcgtat aggcggttaa ggatctcctt
     4801 cgcctgcgcg tcgctgccat aggcggtggc atcgatatat tcgaacgggt ccgcgatgcg
     4861 gtcgtgcttc gattggaacg gctcgccgtc ggccagggcg atccttgttc gcaataccga
     4921 atgccgctcc accagatcgt cgatggcaag tgtcagggcg gtgacatcga tttcgcccgt
     4981 gaggtgcttg cacgacactc ggttccatag cgcgccttgg ggtgacagct tgttcagata
     5041 gagcacgcgt tcctgctgga aggcgagggg gtaggcgcgg acgtcgccgt cgcagtgctc
     5101 gatggcaggg gaagaagccg gactcgtgga catgcacaat ctcctctgga tacggcggct
     5161 actggccatc ctccggtgct aggccggcca ggcggttcgc tctttccttc atcatttcgt
     5221 cggcgatgat caggcgctga atctccgacg ttccgtctcc gatctcggtc agcttggcgt
     5281 cccggaagag gcgttcgagc ggcagatctc tgcaatagcc ataggcgccg tggatctgga
     5341 tggcgtcgcg tgtgacctcc atcgctgtct cggacgcgaa gagcttcgcc tcggaggcct
     5401 gcttggcgta gccttggccg ctgtcgcgca ggcgcgcgcc gtttgccagc agcagctcgg
     5461 cagcgtcgat cttcatttcc atgtccgcca acatgcgctt gacgagctgg aagcggtaca
     5521 gcggctggcc gaactgtcgt ctttgcacgg cgtagtccag cgagagatcc cgcgccgcct
     5581 tcgcaaggcc gattgcaatc gccgccatcc ccagccgggc cgccgacagc gttctctgga
     5641 gaacctcgaa acctctgtcc ttgcgcccga tgagattggt cttggggatg cggacgtcgt
     5701 ggaagacaag ctctccggtg tcggaggctc tcatgccgag cttcttctcc gatttaccgg
     5761 ttgtcagcac gccgggcatg tccttttcca ccagcaacag gctcagcccc atcacgccgg
     5821 cccggcgcga tgtctttgcc accaccacga tgatgtctgc gatgttggcg ttggtgatga
     5881 agagcttcga gccgttcagg atgaagccat cgccatcgtc ttccgcacag gtggtcagcg
     5941 cagagatgtc cgagccgctg ccgctctcgg tcattgcgaa ggcgccgatc ttaacgccgc
     6001 tgaggagcgc tggcagatag cgcgccttca agccctcgct tccggcgctc cagatcggtt
     6061 cgcaggcgag ggtggcgtgc gccaccgtgc tcatggcgac cgcggcggaa tagcgggcga
     6121 tttccccaat caagtcgaga taggtcgcga aattgaggcc gcttccctca tactgctcgg
     6181 gatacggcgt tccgaatatc ccgaagcatc cgatctcgcg gaagatgtcc gccggaaacg
     6241 tctcggtttc atcggcgctg gccgcgaccg gggcgatgac ctttctggcg aacgccgcgc
     6301 cgcttttaag gatgttgctg tctgcgccga tcatgccgct accccgaacg cttgatgcca
     6361 catgtcgaga gcgatcagtg cgtgcgatgt tctgtagacg gcgagatcgt cgatcgtggc
     6421 gaactgtccc ctcttgttga ggaaatcggc cgtcttccgt ttgtcgaaat agttgccgat
     6481 ccgcgaaccg ggcgcctgga cgaggtcgtt cgcatatttc cgctcatgga ggacggtcaa
     6541 cggatcgacg ggaaagggga cgggtctctt cttgcgagca atgacatcgg ccggcagcag
     6601 ctccgcggcg atggctttca acaggatctt ttcgcaatcc gggctcgatt tgtagatcga
     6661 tggcatgtgg acgacatatt cgaccagccg gtggtccaga aacggaacac gcgattcaac
     6721 tccccacgcc atgctcgtct tgtcgagcct
//
LOCUS       DASWPU010000334         3153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_45853,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000334 DASWPU010000000
VERSION     DASWPU010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45853"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VGC27_07150"
     CDS             complement(<1..319)
                     /locus_tag="VGC27_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0282381.1"
                     /translation="MTKPVSNRLAFATRAIHAGQSPDPSTGAVIVPIYATSTYAQESP
                     GVNKGYDYSRSQNPTRMAFERCIADLEDGIQGYAFASGLAAIGTILELLSAGDHVVAT
                     EDIY"
     gene            complement(316..1707)
                     /locus_tag="VGC27_07155"
     CDS             complement(316..1707)
                     /locus_tag="VGC27_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEY0282382.1"
                     /translation="MSKPQRHTARSTLDLIGNTPLVEITHLDTGRCRLFVKLESQNPG
                     GSIKDRIALSMIDAAEQSRRLKPGGTLVEATAGNTGIGLAQVGVLKGYRILLVVPDKM
                     AREKVEHLRALGAEVVMTRSDVGKGHPEYYQDKAQRLAAGIRGSYYVNQFENPANPLA
                     HERTTGPEIFEQMDQDVDAIVVGVGSGGTLTGLGRFFRRVSPKTKMILADPQGSVLAP
                     YINTGKLIAAGSWAVEGIGEDFIPPNADLSLVSEAYSISDKDSIEAARNLLSREGILA
                     GSSSGTLMAAALRFCRAQKEALRVVTLVCDNGNKYLSKVYNDYWAFEQGIQKRALKGD
                     LSDVIARRFESGGTVTVGPDDTLLVAYNRMRAADVSQLPVMSEGRLIGIIDESDILAA
                     VEGLETARAERFAAPVKNSMSAGLQIIQVHDPLDALLPIFARDRVALVCDHDTFVGLI
                     TRIDLINYLRLQG"
     gene            2153..>3153
                     /locus_tag="VGC27_07160"
     CDS             2153..>3153
                     /locus_tag="VGC27_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0282383.1"
                     /translation="MALVNTVDRRNIPELERLVEADHRHLLHPQHHPSEHEQPILWSS
                     GEGPYLVTETGERYLDGLSGMWNVSLGHHRPELIVAATRQLERLAFATAYAGSSHRPA
                     IELAELLSEVVYPSIKAFYFTSGGGDATDTSIRTARYFWRAQGKPEKSKIIARQLSYH
                     GSTIGSASATGVAEFSDGFGPRLPGFLHIESPYPYRFENKTPDKTPGVAAADLLEEAI
                     LREGAETVAAFIAEPVQGGGGGVIVPQDDYFPRIREICDRYDVLLISDDVITGFGRTG
                     RWFGLEHWDVQPDIVQFAKGITSGYIPLGGVGVSGRIKDVLDHADPARRWWHGYTYSG
                     "
BASE COUNT          567 a          981 c          986 g          619 t
ORIGIN      
        1 cgtagatgtc ctcggtcgcg accacatggt cgccggccga caagagctcc agtatcgttc
       61 cgatcgccgc cagtccggag gcgaaggcgt aaccttgaat gccgtcttcc aagtcggcga
      121 tgcagcgctc gaaagccatc ctggtggggt tctggctgcg cgaataatcg tatcccttgt
      181 tcacgcccgg gctttcctgg gcataggtcg aggtcgcgta aatgggaaca atgacggcac
      241 cggttgaggg atctggcgat tggcccgcat gaatggcccg cgtggcgaac gccaggcggt
      301 tggacaccgg ttttgtcatc cctgcagcct cagataattg atcaggtcga tgcgggtgat
      361 gaggcccacg aacgtatcgt gatcgcaaac cagcgcaacg cgatcgcgcg caaagatcgg
      421 cagcagggcg tcgagcgggt cgtggacctg tatgatctga agacccgccg acatcgagtt
      481 tttcacggga gcggcaaagc gttccgcgcg cgcggtttcg agtccctcca ctgcggccag
      541 gatgtcgctt tcgtcgatga tgccgatgag ccggccttcc gacatgacgg gaagctggga
      601 cacgtccgcg gcgcgcatcc ggttgtaggc gaccagcagc gtgtcgtcgg gtcccaccgt
      661 gacggttcca ccgctttcga accgcctggc gatcacgtcg ctgagatcgc ctttgagcgc
      721 gcgtttctgg atgccctgct cgaacgccca ataatcgtta tagactttcg aaagatactt
      781 gttgccgttg tcgcaaacga gcgtcaccac ccgcagcgcc tccttttgcg cccggcaaaa
      841 gcgcagcgcc gccgccatca gggtgccgga cgacgatccc gccagaatgc cttcccggga
      901 caggaggttt cgcgcggcct ctatgctgtc cttgtcggag atcgaatagg cctcggacac
      961 cagcgacagg tccgcgttcg gcgggatgaa atcctcgcct atgccttcca ccgcccagga
     1021 acccgctgcg atgagcttgc cggtattgat gtagggcgcg agcaccgagc cttgcggatc
     1081 ggcgaggatc attttggtct tgggcgaaac ccgccggaaa aaccgcccca gacccgtcaa
     1141 ggtgccgccg gagccgacgc cgaccacgat ggcatcgaca tcctgatcca tctgctcgaa
     1201 gatttccggt cccgtggtcc gttcgtgcgc aagcggattg gcgggatttt cgaactggtt
     1261 gacgtaatag gagccgcgaa tgccggccgc caaacgctgt gccttgtcct ggtaatattc
     1321 ggggtgtccc tttcctacgt cggaacgggt catgacgact tcggcgccca gcgcacgcag
     1381 atgctcgacc ttctcgcgcg ccatcttgtc cggcacaacc agcaggatgc gatagccctt
     1441 cagcaccccg acctgggcaa gtccgattcc ggtattgccg gccgttgcct cgacgagcgt
     1501 gccgccgggc ttcagccgcc gggactgctc tgcggcatcg atcatcgaca gagcgatgcg
     1561 gtccttgatg gagccgccgg gattctggct ttcgagtttc acgaagaggc ggcatcggcc
     1621 ggtatccaga tgggtgatct cgacaagcgg cgtattgccg atgaggtcga gcgtggatcg
     1681 cgccgtgtgt cgttgtggct tgctcattcg atgactcttt tgtttgagcg ggatgttccc
     1741 cgcgcgcggg cgcagcctgc gacatgctgc aggggccgaa ccccgatttt ccacaataca
     1801 atacaaagcc tatagggtat atctagaaga aactccaagg agcgtcaatt ttgcttgacg
     1861 gtgcccggcc aggtaaggaa gatggcttga cttctcgcga gtgcggccgt gtcggagcat
     1921 tgtcattccc tttggatcga agtggcggga ttctccggtt ttgcactgta tttaaccgcg
     1981 gttgccggat cggccgatcc gtgtatgggg gcggtggcct aaggccatcg ccggaacgca
     2041 ctgcggtcgc cgatcccgcg gggcggcaaa tcccgccatt cgccgttcga gccgcgcacg
     2101 aagctgtgtg ccgttcgatc gctattacgg tccgtccggc cggaggagag ccatggcact
     2161 cgtgaacacc gtcgatcgcc gcaacatacc tgaactggag cgcctggtcg aggccgatca
     2221 ccggcatctg cttcatccgc agcatcatcc ctccgagcac gagcagccca ttctctggtc
     2281 gagcggggag gggccttacc tcgtcaccga aaccggcgag cgctatctcg acggtctcag
     2341 cgggatgtgg aacgtcagtc tcggccatca caggccggaa ttgatcgtcg cggcgacgcg
     2401 ccagctcgaa cggctcgctt tcgcaacagc ctatgccggc tccagccacc gtccggcgat
     2461 cgaacttgcc gagttgcttt cggaggtcgt gtatccctcg atcaaggcct tctacttcac
     2521 gagcggcggc ggtgacgcga ccgacacgtc gatccgcacg gcccgctatt tctggcgcgc
     2581 gcaaggaaaa ccggagaaat cgaagatcat cgcgcgccag ctttcctatc atggctcgac
     2641 gatcggctcg gccagcgcca cgggcgtcgc cgagttttcc gacgggttcg gtccacgcct
     2701 gccgggcttc ctgcatatcg agtctccgta cccgtaccgc ttcgagaaca agacgccgga
     2761 caagacgcca ggcgtcgccg cggccgatct cctcgaagaa gcgatcctgc gcgagggagc
     2821 cgaaacggtg gcggccttca ttgccgagcc cgtgcagggc ggagggggcg gggtgatcgt
     2881 gccccaagat gattatttcc cccgcatccg cgagatctgt gaccgatatg acgtgcttct
     2941 catcagcgac gacgtcatca ccggcttcgg tcgtacgggc cgctggttcg ggctggagca
     3001 ttgggatgtc cagcccgata tcgtccaatt cgcgaaaggc atcaccagcg gttacattcc
     3061 gctgggcggc gtaggcgtca gcgggcgcat caaagacgtg ctggatcacg cagacccggc
     3121 ccgccgctgg tggcacggct atacctactc ggg
//
LOCUS       DASWPU010000335         3131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_39970,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000335 DASWPU010000000
VERSION     DASWPU010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39970"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1387
                     /locus_tag="VGC27_07165"
     CDS             104..1387
                     /locus_tag="VGC27_07165"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY0282384.1"
                     /translation="MSLHEAADEPLAAEMSVLGIAARDAARVLRETTAEAKDAALLAA
                     AAAIRVHAADILAANGEDMTLAKMEGLGPALLDRLALDEGRVEAMARGVEEVAALPDP
                     VGRELARWTRPNGLEIARLATPIGVIGIIYESRPNVTADAAAICLKSGNAVILRGGSE
                     SARSSGAILEALREGLASAKLPADAVQMVPTTDRAAVGMMLTGLDGAIDLIIPRGGKG
                     LVGRVQSEARVPVLAHLEGICHVYIHEAADLEKARAIVLNAKMRRTGICGAAETLLVD
                     RSVLQSHGLPVLEDLSAAGCEIRGDQEVCAAYHLAKAASEEDWRTEYLDAIISVKAVG
                     GIEEAIRHIETYGSHHTEAIVTEDTAAAERFLWALDSAIVIWNASTQFADGGEFGMGA
                     EIGIGTGKLHARGPVGVEQLTTFKYIVCGNGQVRA"
     gene            1601..2209
                     /locus_tag="VGC27_07170"
     CDS             1601..2209
                     /locus_tag="VGC27_07170"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY0282385.1"
                     /translation="MEWLRSPGPVARGLRIGLLGGSFNPAHEGHLYVSEVARKRLGLD
                     YIWWLVAPQNPLKPENGMAPLEDRLAAARALAGRNPRLIVTDIERRMGTRYTVDTLKA
                     LTCRFPGVRFVWLMGSDNLDQFHRWRRWEEIVRRVPVAIVERPGSILAPLRAKAMQRF
                     AWSRAPALRPPPAMIVVDGRRNEASASAIRAGLGGREALVLE"
     gene            2265..2753
                     /gene="rsfS"
                     /locus_tag="VGC27_07175"
     CDS             2265..2753
                     /gene="rsfS"
                     /locus_tag="VGC27_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY0282386.1"
                     /translation="MRLNGKRRTNLTRKTTKPAKKPAAKKPAAKKAAPAKKSAPKKLR
                     APATVLLSRVLASLSDDKAEQVATIDMEGRSSLCDTIVIASGRSSRHVASIAEHLARR
                     LKEAGYGTCHIDGLTQGDWVLVDAGDVIVHVFRPEVRAYYDLEGMWSVDEQPARARRA
                     AR"
     gene            2781..>3131
                     /locus_tag="VGC27_07180"
     CDS             2781..>3131
                     /locus_tag="VGC27_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY0282387.1"
                     /translation="MKGSFILSLSEGKDQSMRLTVLAVGFARSTSEGALIDDYIHRAL
                     LMGRNMGFSSVAVEEVSLSKARDAKMRMAEEAEKLAARVPAGAHVVLLDAKGKGMTSE
                     DFSEMLGALRDAGTK"
BASE COUNT          552 a         1010 c         1071 g          498 t
ORIGIN      
        1 tcgcgggcaa gcgcagcctc gaaatcgagg ccattctcgg ctatcgtggc cgcgacgaaa
       61 tgatccatcg cgacgatctg gcgctgacgg gaatgggcga aaaatgagcc tgcacgaagc
      121 cgcggacgaa ccgcttgccg ccgagatgag cgtgctcggc atcgccgcgc gcgacgccgc
      181 gcgcgtcttg cgcgagacta cggccgaggc gaaggacgcg gcactgctgg ccgccgccgc
      241 cgccatccgg gtgcatgcgg cggacatcct ggcggccaac ggcgaagaca tgaccctggc
      301 gaaaatggag ggcctcggtc cggccttgct cgaccgtctg gcgctcgacg aagggcgcgt
      361 ggaagcgatg gccaggggcg tggaagaagt ggcggcgctg cccgatccgg tgggccggga
      421 gctggcgcgc tggacgcggc cgaacggcct cgaaatcgcg cgcctcgcca cgcccatcgg
      481 ggtgatcggc atcatctatg agagccggcc gaacgtcacc gcggatgccg ccgccatctg
      541 ccttaagtcc ggcaacgcgg tgatcctgcg cggcggctcg gaatccgcgc gttcgtcggg
      601 agcgatcctc gaagcgctac gcgaaggatt ggcgtccgcg aagcttcccg ccgatgccgt
      661 gcagatggtg ccgacgacgg accgcgccgc cgtcggcatg atgctcacgg ggctggacgg
      721 cgccatcgat ctcatcatcc cgcgcggcgg caaggggttg gtcgggcggg tgcaaagcga
      781 agcgcgggtg ccggtgctgg cgcatctgga aggcatctgc cacgtctata tccacgaagc
      841 ggccgacctc gaaaaggcgc gcgccatcgt gctcaacgcc aagatgcggc gcaccggcat
      901 ctgcggcgcg gcggaaacgc ttcttgtgga ccgctcggtc ttgcaatcgc atggtctgcc
      961 ggtgctggag gatcttagcg ccgccggctg cgaaatccgc ggcgaccagg aggtgtgcgc
     1021 cgcgtaccac ctggccaagg cggcgagcga agaggattgg cgcaccgaat atctcgacgc
     1081 catcatctcg gtgaaggccg tgggcggcat cgaagaagcg atccgccaca tcgaaaccta
     1141 cggctcgcat cacaccgaag ccatcgtcac cgaggacacc gccgccgccg aacgcttcct
     1201 ctgggcgctc gattccgcca tcgtgatatg gaacgcctcc acccaattcg ccgacggcgg
     1261 cgagttcggc atgggcgcgg aaatcggcat cggcaccggc aagctgcatg cccgcggccc
     1321 ggtcggcgtg gagcagctca ccacgttcaa atatatcgtg tgcggcaacg gacaagtacg
     1381 tgcgtgaggg aattgccaaa atctttgcct ccccccttga gtccggcttc gccaaggctt
     1441 cgccggactg tcgcgaaccc gccgaagcca agcgaaggcg ggttgagggg gaggcggaaa
     1501 aatccgcgat gcggattttt cggtgggggg taatgtcggc aatacccctc cccgaaattt
     1561 gctctgcaaa tttcgaccct ccctcaaggg gagggtgaag atggagtggc tccgctctcc
     1621 cggccctgtc gcgcgtggct tgcgcatcgg cctgttgggc ggctcgttca atccggcgca
     1681 tgagggccat ctctatgtga gcgaggtggc gcgcaaacgg ctcggcctcg attatatctg
     1741 gtggctggtg gcgccgcaga acccgctgaa gccggagaac ggcatggcgc cgctggagga
     1801 ccgtctggcc gcagcgcggg cgctggccgg ccgcaacccg cgcctgatcg tcaccgacat
     1861 cgagcgcagg atgggtaccc gttacacggt cgacacgctg aaggcgctta cttgccgctt
     1921 tccgggagtt cgtttcgttt ggctgatggg cagcgataat ctggaccagt tccaccgctg
     1981 gcggcgttgg gaggagatcg tccggcgggt tcccgtcgcc attgtggagc gtccgggcag
     2041 catcctggcg ccgctccgtg ccaaggcgat gcagcgtttt gcctggagcc gcgcgccggc
     2101 cctgcgcccg ccgccggcga tgatcgtggt ggacgggcgg cgcaacgaag ccagcgccag
     2161 cgcgatccgc gcgggccttg gagggcgcga agccctcgtg ctagagtgat ttccgtaacg
     2221 gcaaaggagc gagcggctga gcgaagcagg aatccgtccg ccttttgcgc cttaacggca
     2281 aacggaggac taatctgacc cgcaagacga ccaagccggc caaaaagcct gccgccaaga
     2341 aacccgccgc aaagaaggcg gcccccgcca agaaatccgc cccgaaaaag ctgcgtgcgc
     2401 cggcaaccgt gttgctttcc cgcgtcctgg cttcgctcag cgacgacaag gccgaacagg
     2461 tcgcgaccat cgatatggaa ggccgctcct cgctctgcga tacgatcgtg atcgccagcg
     2521 ggcggtcgtc gcgccatgtg gcctccatcg ccgagcatct ggcgcggcgg ctgaaggagg
     2581 ccggctacgg cacctgtcac atcgacggcc tgacgcaagg cgactgggtt ctggtcgatg
     2641 ccggcgacgt gatcgttcat gtcttccgtc ccgaagtgcg ggcctattac gacctcgaag
     2701 gcatgtggtc ggtggacgag cagccggcgc gcgcgcgccg cgccgcgcgg tagtggcctc
     2761 catgcttcga caagctcagc atgaagggtt ccttcatcct gagcctgtcg gagggtaagg
     2821 accaatcgat gcgtctgacc gtcctggccg tcggtttcgc ccgcagcacc tccgagggcg
     2881 cgctgatcga cgattacatc catcgcgccc tcctgatggg ccgcaacatg ggattttcct
     2941 ccgtcgcggt ggaagaagtc tccctctcca aggcgcgcga tgccaagatg cgcatggcgg
     3001 aagaagccga aaagctggcc gcgcgcgtac cggccggcgc gcatgtcgtt ctgctcgacg
     3061 ccaagggcaa gggcatgacc agcgaggatt tctccgagat gctgggcgcg ctgcgcgacg
     3121 cgggcaccaa g
//
LOCUS       DASWPU010000336         5238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_64327,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000336 DASWPU010000000
VERSION     DASWPU010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64327"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VGC27_07185"
     CDS             <1..496
                     /locus_tag="VGC27_07185"
                     /inference="COORDINATES: protein motif:HMM:NF018652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282388.1"
                     /translation="LSASFYEATQEITARAGLPAYEVSNHARPGCESRHNLLYWRYGS
                     YAGLGPGAHGRLELNGKHIATVCERLPERWREKVARNGHGFMEISEIDERTGAREHLL
                     MNLRLAEGIDLAAYRARWGGAPSPERIAALAADGLVKLESGRLAATPRGMLVLNRLIA
                     ELAD"
     gene            complement(493..1731)
                     /locus_tag="VGC27_07190"
     CDS             complement(493..1731)
                     /locus_tag="VGC27_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY0282389.1"
                     /translation="MFVPAASFRFALRRLCAIACTVAVVALTGCASTPAPPPAPPIPV
                     QPPAPHQLSADQPDFFRLPNLAPDKIPVRVGIILPFSDSSASVRALANAMMKSAELAL
                     YDSGNRDILLMAADDTGSSADAAAAAQKLLNQGAEIIVGPIFAASVAAVAPLARDRGV
                     PVLAFSTDRTVAGNGVYLLSFQPQSEVKRVIAYAAGQGHKTLAGLIPQTPYGDVVEQS
                     FREAAAAAGASIVDVERFNPSAGAIMDPVAAVAKSGCDAIFIAQGGSLLRSIAPALVY
                     AGVDNTKVKYLGTGLWSDPATLKEPLLTGGWFAAPEPGTNDAFSAKYRQTFGTDPPQL
                     ASLAYDAISLVALLAPGTPYHRFTLGALIDPNGFSGASGIFRFRQDGSIERGLAVLAV
                     SPGGFTVVSPAPTTFQASGS"
     gene            1797..2639
                     /gene="rsmI"
                     /locus_tag="VGC27_07195"
     CDS             1797..2639
                     /gene="rsmI"
                     /locus_tag="VGC27_07195"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY0282390.1"
                     /translation="MAAGLYVTATPIGNAQDVTLRALDVLEGADLIAAEDTRVTAKLL
                     AIFGIAKPLTSYNDHNAARERPRLLARLKEGARVALVSDAGTPLVSDPGYKLVREALA
                     AGIEVHAIPGASAPLVALALAGLPTDRFFFAGFLPSKRGERRHTLEELKTIRATLIFF
                     EAPQRLAESLADMAEIFGARPAAVARELTKLHEDVRRGDLASLAEAYAGTPPRGEITL
                     AVGPAGEAETDLARVDAALTKALPFMPVRAAVDLVAEMLGAPRREVYARALAKKNGGQ
                     DGTG"
     gene            2617..2997
                     /locus_tag="VGC27_07200"
     CDS             2617..2997
                     /locus_tag="VGC27_07200"
                     /inference="COORDINATES: protein motif:HMM:NF009151.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY0282391.1"
                     /translation="MAKTERVERHKQAEKRGRHGETLAALLLRCKGYRILGRRVRTRA
                     GEIDLVAKSPSGIVCFVEVKARPQDGLAAEAVAPRQRARIARAAELYLAGRQAAKGVR
                     FDVVTVVPGRLPRHLRDVWSADGL"
     gene            3081..4031
                     /gene="gshB"
                     /locus_tag="VGC27_07205"
     CDS             3081..4031
                     /gene="gshB"
                     /locus_tag="VGC27_07205"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY0282392.1"
                     /translation="MSLVVAIQMDPIERFKIDADSTFALMLEAQVRGHTLLCYGPHEL
                     TFREGKVTARARPVEVRPVKGDHFTLGEASVIDLSGVDVVLMRQDPPFDMAYITATHI
                     LERLQPGTAVFNDPKEVRNAPEKLFATLFSGLMPPTLITSDAAEIAAFREAQRDIILK
                     PLYGNGGAGVFRLGPDDENLGSLLEMFATFYREPIMVQRYLPQVRQGDKRIILIDGEA
                     AGAINRVPAKGEARSNMHVGGKPEPTTLTPRDMEICAALGPELKKRGLIFAGIDVIGD
                     YLTEINVTSPTGIQEVKRFGGPDGASLIWNAIERRLKARG"
     gene            complement(4572..>5238)
                     /locus_tag="VGC27_07210"
     CDS             complement(4572..>5238)
                     /locus_tag="VGC27_07210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEY0282393.1"
                     /translation="MREARRDFDRFDAVCDHLLVVDRSLTDEDGQPAVVGTYRLMRDV
                     DAVRAGGFYTATEYDISAMLAGLPADTHLLELGRSCVLKAYRSKTGTMQLLWRGLMVY
                     VTRFSIDLMFGCASFAGTDPKLLALPLSYLHHYHPMPDAVRVRAIPSQYVEMNLMPKE
                     AVDPKEALRALPPLLKGYLRAGVCIGDGAVIDRQFGTTDVFIYCPVANIDARYRNRFG
                     MSC"
BASE COUNT          974 a         1636 c         1756 g          872 t
ORIGIN      
        1 cctgtcggcg tccttctatg aagcgacaca ggagattacc gcgcgcgccg gcttgcccgc
       61 ttacgaggtt tccaatcatg cccgccccgg ctgcgagagc cggcacaatc ttctctactg
      121 gcgctatggc tcatatgccg gcttgggtcc gggcgcgcat ggcaggctgg aactgaacgg
      181 caagcacatc gccaccgtgt gcgaacgtct gccggaacgc tggcgcgaga aggtggcgcg
      241 caacggccac ggctttatgg aaatcagcga gatcgacgaa cgcaccggcg cacgcgagca
      301 tctgctgatg aatctgcgct tggccgaggg gatcgatctt gcagcctatc gcgcccgctg
      361 gggcggcgcg ccttcgccgg aacgcatcgc ggcactggcg gcggacgggc tggtcaagct
      421 cgaatccggc aggcttgcgg cgacgccgcg cggcatgctg gtgctcaacc ggctgatcgc
      481 agaactggcc gattaagaac cgctcgcttg gaacgtcgta ggcgcggggc tgaccaccgt
      541 gaagccgccc ggactgacgg ccagaaccgc caagccgcgc tcgatcgatc cgtcttgccg
      601 gaaccggaag atgccgctgg cgccggaaaa gccgttggga tcgatcagcg cccccaaggt
      661 aaagcgatga tacggcgttc cgggtgccag gagcgccacc agcgatatgg cgtcatatgc
      721 caatgatgcc aattggggag ggtccgtgcc gaaggtctgg cgatatttcg cgctgaaggc
      781 gtcgttggtg ccgggctccg gcgcggcgaa ccagccgccg gtcaaaagcg gttccttgag
      841 ggtggcgggg tcggaccaaa gcccggtgcc gagatatttc accttggtgt tgtccacgcc
      901 ggcatagacc agcgcgggtg cgatgctgcg caacagcgag ccgccctggg cgatgaaaat
      961 cgcatcgcag ccggatttgg cgacggccgc caccgggtcc atgatcgcgc ctgcgctggg
     1021 attgaagcgc tccacgtcga cgatgctggc gcccgcggcg gcggcggcct cacggaacga
     1081 ttgttcgacg acatcgccat agggcgtctg cgggatcagg ccggcgagag tcttgtgacc
     1141 ttggccagcg gcataggcga tgacgcgctt gacctcgctc tgaggctgaa agctcaagag
     1201 atagacgccg ttgcccgcca ccgtgcggtc ggtcgagaaa gcgagcaccg gcacgccgcg
     1261 gtcgcgggca agcggggcaa ccgcggccac cgaggcggca aagatgggcc cgacgatgat
     1321 ttccgcgccc tggttgagca gtttctgggc cgccgctgcg gcgtccgcgg acgaaccggt
     1381 gtcgtcggcc gccatcaaca ggatgtcgcg attgccggaa tcgtagaggg caagctcggc
     1441 cgatttcatc atggcattgg ccagggcgcg cacgctcgcc gaggaatccg agaagggtag
     1501 aatgatgccg acgcgcacgg ggatcttgtc cggcgccaga ttgggcagcc tgaagaagtc
     1561 cggctggtcg gcggaaagct gatgcggggc cgggggctgg accggtatcg gcggggccgg
     1621 cggcggtgcg ggggtcgagg cgcagccagt caaagcgact accgcaacgg tgcaggcaat
     1681 ggcacaaagg cggcggaggg cgaaacggaa cgatgcggca ggcacaaaca tgggacgctc
     1741 cttcacgcgg cgagcctaag cactcgcggc cccggcgtaa atccggaata agcgaattgg
     1801 cggcagggct ttacgtcacc gccaccccga tcggcaatgc gcaggacgtc actttgcggg
     1861 cgctcgacgt gctggaaggc gcggacctga tcgccgccga agatacccgc gtcaccgcca
     1921 agcttctggc catcttcggg attgccaagc cgctgacctc ctataacgac cataacgcgg
     1981 cgcgcgagcg ccccaggctg ctggcgcgac tgaaagaggg cgcgcgtgta gccctggtca
     2041 gcgatgcggg cacgcctttg gtttccgatc cgggctataa gctggtgcgc gaggcgctgg
     2101 ccgcgggcat agaggtgcat gccattcccg gcgcgtcggc gccgctcgtc gcccttgccc
     2161 tggcggggct gccgacggac cgctttttct tcgccggctt tttgccgtcc aaacgtggcg
     2221 agcgccgtca cacgctggaa gagctgaaaa ccatccgcgc caccctgatt tttttcgaag
     2281 ccccccagcg cctggcggaa agcctcgccg acatggctga gattttcggc gcgcgcccgg
     2341 cggcggtggc gcgcgaattg accaagctgc acgaagacgt gcgccgcggc gatctcgcca
     2401 gcttggctga ggcctatgcc ggaacgccgc cgaggggcga aatcaccctc gcggtggggc
     2461 ccgccggaga agccgagacc gatcttgcgc gcgtcgatgc ggcgctcacc aaggcgctcc
     2521 ccttcatgcc ggtgcgcgcg gcggtcgatc tggtggccga aatgctgggg gcgccgcgcc
     2581 gcgaggttta cgccagagca ctcgccaaga aaaacggtgg ccaagacgga acgggttgag
     2641 cgtcacaaac aggccgagaa gcgcggccgc cacggcgaaa cgctcgccgc gctgctgctg
     2701 cgctgcaagg gctatcgcat cctgggccgg cgcgtgcgca cccgcgccgg tgaaatcgat
     2761 ctggtggcaa aaagcccgtc cggcatcgtc tgctttgtgg aagtcaaagc gcggccgcaa
     2821 gacgggcttg ccgccgaagc ggtggccccg cgccagcgcg cccgcattgc acgcgcggcg
     2881 gaactttatc tggctgggcg ccaggccgcc aaaggcgtgc gcttcgatgt cgtcaccgtg
     2941 gttccggggc ggctgccgcg ccatctgcgc gacgtttgga gcgcggacgg gctgtgaccg
     3001 ggccttgtag aaccgggccg gaacggcgat ctatagcttg gtgtccccag gccccgggat
     3061 aaaagcatga gtttaaaagc atgagtttag tcgtcgccat ccagatggac ccgatcgagc
     3121 gtttcaagat cgacgccgat tcgactttcg ccttgatgct ggaagcccag gtgcgcggcc
     3181 atacgctgtt gtgctatggc ccgcacgaac tcactttccg cgaaggcaag gtcacggcgc
     3241 gcgcccgtcc ggtcgaggtg cgtccggtta agggcgatca tttcaccctg ggcgaagcgt
     3301 cggtgatcga tctgtccggc gtcgatgtgg tgctgatgcg ccaggacccg cccttcgaca
     3361 tggcctacat caccgccacc cacattctgg agcggctgca gccgggaacg gcggtgttca
     3421 acgatcccaa ggaagtgcgc aacgcgcccg aaaaactctt cgcgaccctg ttcagcggct
     3481 tgatgccgcc gaccctgatc acctccgacg ccgccgaaat cgccgccttc cgcgaggcgc
     3541 agcgcgacat catcctgaag ccgctttacg gcaatggcgg agcgggcgtc tttcgcctcg
     3601 ggccggacga cgagaatctg ggttccttgc tcgaaatgtt cgccactttc tatcgcgagc
     3661 cgatcatggt gcagcgctat ctgccgcagg tgcgccaggg cgacaagcgc atcatcctga
     3721 tcgacggcga agcggccggc gcgatcaacc gcgtgcctgc caagggcgag gcgcgctcca
     3781 acatgcatgt cggcggcaag ccggaaccca ccacgctgac gccgcgcgac atggagattt
     3841 gcgcggcgct cggcccggag ttgaaaaagc gcggcctgat cttcgccggc atcgacgtga
     3901 tcggcgatta tctcaccgag atcaacgtca cctcgcccac cggcattcag gaagtcaaac
     3961 gcttcggcgg cccggacggc gccagcctga tctggaatgc gatcgaacgg cgtctgaagg
     4021 cgcgcggtta agcggatttt cgtcaaggcg atccacgaaa cctccgtgtc atggtccgca
     4081 tatgcgggcc atgacacgca agtttatgat cccgcttggc tcaacctgtt ctggagcgtg
     4141 atccatctat atgggatcgc gccacactca tgcttcgaca gtcggtagca acagcgtgcc
     4201 gaccagcatg agggattagg catccccctc accctgagct tgtcgaaggg tgactggagc
     4261 gattcagcca gggtggatcg tgctctacag caggttggaa tcaaatggac tcaacatctc
     4321 acctgtcacg gcccgcaagt tcgtagaggc agcgtgcgaa cgggccgccc aggtgaaact
     4381 ggttcatgcc ttccaagaaa agcacggcac aattccatcg gcaaagagca attgtcatct
     4441 ggatggcctg cttccctcct tcgccaaggc ttcggagggt ctgcgcctgt cctgcgaagc
     4501 tgcgaagcag cgtaggagga ttgcaggcca tgacatggtg tttccgtgcg tcatcttgat
     4561 gacagacaac tctagcagct catcccgaac cggttacgat agcgcgcgtc gatgttcgcg
     4621 accgggcaat agatgaacac gtcggtggta ccgaattgcc ggtcgatcac cgcgccgtcg
     4681 ccgatgcaca cgcctgcccg cagataccct ttgagcaaag gcggcagcgc acgcagcgcc
     4741 tctttcggat cgacggcctc cttcggcatg aggttcattt cgacatattg cgaaggaatg
     4801 gcgcgtacgc ggacggcgtc cggcatcggg tggtaatggt gcagatagga caagggcagc
     4861 gccagaagct tgggatcggt tcccgcgaag gaggcgcagc cgaacatcag gtcgatggag
     4921 aagcgcgtca catagaccat caagccgcgc cacaggagct gcatggtgcc ggtcttgctg
     4981 cgataggcct tgagcacgca ggagcggccg agttccagaa ggtgggtgtc ggccggtagg
     5041 cccgccagca ttgccgaaat gtcatattcg gtggcggtgt aaaacccgcc ggcccggacg
     5101 gcgtcgacat cgcgcatcag ccggtaggtg ccgaccacgg cgggctgtcc gtcttcgtcg
     5161 gtgagcgagc ggtcgaccac caggagatga tcgcaaaccg catcgaaacg gtcgaagtcg
     5221 cggcgcgctt cgcgcatg
//
LOCUS       DASWPU010000337         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_70526,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000337 DASWPU010000000
VERSION     DASWPU010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70526"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="VGC27_07215"
     CDS             complement(<1..1841)
                     /locus_tag="VGC27_07215"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0282394.1"
                     /translation="MRQFSKSWIATLFLGVLTLSFVTWGIGDMFQGSTSTAVATVGGV
                     GIEQTEFQRDYNNFIQRQGNLTAEQARRANLGSIMLQQNIAQVALDNVARHLRMTVSD
                     EMVSERIRSIPEFVGISGTFDRSVFQQKIGQVGYSEQGFIEAIRHDMARAQLLHAIEG
                     GFEVPTGYARALFAYYTEVRAAEYVTVDAKALPPIATPSDAVLSAYLKAHADRFSTPE
                     YRDVTYAEISPDDVSKDVTVTDQQIQTVYDAAKDKYIIPERRDLEQISFPSKAEAQAA
                     HARIAAGTSFAAIAASRGLAQSDLGLGSRVAGDLDPVEAKIVFALPEGEVSEPIQVTF
                     GWVLVRVVKITPGSVTTLDQARDEIQQNLLRQLTDAKMVDVTNAYNDAVRRGATLTEA
                     AEKVGMRPGRVVAMDGNGLAPDGSKAAAPDDPVFRAQAFKAETGEEGDPFQGKSGSYF
                     VLSVNAVVPPKLEPFEKVRARVIAEWTGEQRAILLKKKATELTAQANREMSIEGAARE
                     IGAKVQASPALTHGTNDATFSSELVRALFAVAPGAAAYGPLGKGEGYIVARVTGIYHP
                     LPPTNSLEFQQGVRAISQNIAEDVVASFAHAARDKQGVKINDKLLATV"
     gene            1945..>2587
                     /gene="tpiA"
                     /locus_tag="VGC27_07220"
     CDS             1945..>2587
                     /gene="tpiA"
                     /locus_tag="VGC27_07220"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY0282395.1"
                     /translation="MLIAGNWKMNGLSSALDEVDALIRALKARPAGLDVLVCPPATLI
                     SRLAARAGGVVAVGGQDCHPQPSGAFTGDVSAEMLRDAGAAWTIVGHSERRQYHGETD
                     TLVAAKAEAAWRGGLGVIICIGETEAERNAGREEEVVDRQLAASVPDGAAAARTAIAY
                     EPIWAIGTGKTPTNEDIARMHVHLRGELIKRFGGSGRAIPILYGGSVNPKNAKE"
BASE COUNT          449 a          829 c          834 g          475 t
ORIGIN      
        1 acggtggcga gcagcttgtc gttgatcttc acgccttgtt tgtcgcgcgc ggcatgggcg
       61 aaagacgcaa cgacgtcttc tgctatgttt tgcgaaatcg cgcgcacgcc ctgctggaat
      121 tccaggctgt tggtcggcgg caagggatga tagatgccgg tgacgcgcgc cacgatatag
      181 ccttcgccct tgcccagcgg accgtaagcg gcggcacccg gagcgaccgc gaacagcgcg
      241 cgcaccagct cgctcgagaa tgtcgcatcg ttggtgccgt gggtcagcgc ggggctggcc
      301 tgcacctttg ctccgatctc tctggccgcg ccctcgatcg acatttcccg atttgcttgc
      361 gcggtgagtt ccgtagcctt cttcttcagt agaattgcgc gctgttcgcc ggtccattcg
      421 gcgataaccc gcgcccgcac cttttcgaac ggctcgagct tcggcggcac gacggcattc
      481 accgaaagga caaaatagct gcccgatttg ccctggaagg gatcgccctc ttcgccggtc
      541 tcggccttga acgcctgcgc acggaagacc ggatcgtcgg gggcggcggc cttgctgccg
      601 tcgggcgcca agccgttccc atccatcgcc acgacgcggc ccggccgcat gccgactttc
      661 tcggccgcct cggtgagcgt ggccccgcgc ctcacggcat cgttgtaggc gttggtgacg
      721 tcaaccatct tggcgtcggt caattgccgc aacaagttct gctggatttc gtccctggcc
      781 tgatcgagcg tcgtgacgct gcccggcgtg atcttgacga ctcttaccag cacccagccg
      841 aaggtaacct ggatcggctc gctcacctcc ccttccggca gcgcaaagac gatcttcgct
      901 tccacaggat cgagatcgcc cgcgacccgc gagcccagtc cgagatcgga ttgagcgaga
      961 ccgcgtgacg ccgcgatcgc ggcaaagctc gtccccgcgg cgattctggc gtgcgccgct
     1021 tgcgcttcgg ctttgctggg gaacgaaatc tgctccagat cgcgcctttc ggggatgatg
     1081 tacttgtcct tggccgcgtc atagacggtc tggatctgct gatcggtgac ggtaacatcc
     1141 ttggacacgt cgtcggggct gatttcggcg taggtcacgt cgcggtattc gggcgtcgaa
     1201 aagcgatcgg catgagcctt caaataggcg ctcagaacgg cgtccgaagg cgtcgcgatc
     1261 ggcggcagcg ctttggcgtc caccgtcaca tattccgcgg cccggacttc cgtataataa
     1321 gcgaacaacg cccgcgcata gccggtgggc acttcgaagc cgccctcgat cgcgtgcaac
     1381 aattgcgcgc gggccatatc gtgccgtatc gcctcgatga agccctgctc gctatagccg
     1441 acctggccga tcttctgctg aaaaacgctg cgatcgaacg taccggagat gcccacgaac
     1501 tccgggattg agcggatccg ctccgacacc atctcgtcgc tcaccgtcat ccgaagatga
     1561 cgcgccacat tgtccagcgc gacctgggca atgttctgct gcagcatgat cgagccgagg
     1621 ttggcgcggc gggcctgttc cgcggtcagg ttgccctggc gttgaatgaa attgttgtaa
     1681 tcgcgctgga attcggtctg ttcgatgccg accccgccga cggtcgcgac cgccgtgctg
     1741 gtgctgccct ggaacatatc gccgatgccc caggtcacga aactgagcgt cagaacgccc
     1801 aggaacaggg tggcaatcca ggacttcgaa aactgacgca tttgctggag catggcggac
     1861 ccgatgatgt ggcggcgaaa ggcgcgtggc ggcgagaagc ggcggatgat agggagaggg
     1921 cctatcgacg gcaaggagaa gggcatgttg atcgcgggaa actggaaaat gaacggctta
     1981 tcgtccgccc tggacgaggt cgatgcgctg attcgcgcgc tcaaggcccg cccggccgga
     2041 ctggatgtgc tggtgtgccc gccggcgacg ctgatttcgc ggctggccgc ccgggcgggc
     2101 ggcgtggttg cggtgggcgg tcaggactgc catccgcagc cctcaggcgc cttcaccggc
     2161 gatgtttcgg ccgaaatgtt gcgcgacgcg ggagccgcct ggaccatcgt cggccattcc
     2221 gagcgccgcc agtaccatgg cgaaaccgac acgctggttg ccgccaaggc cgaggccgcc
     2281 tggcgcgggg ggctcggcgt tatcatctgc atcggcgaga ccgaggccga gcgcaacgcc
     2341 ggccgggaag aagaggtcgt cgaccgccag cttgccgcca gcgtgccgga cggcgccgcg
     2401 gcggcacgca cggccatcgc ctacgaaccc atctgggcca tcggcaccgg gaagacgccc
     2461 accaacgaag acatcgcccg catgcacgtt catctgcgcg gcgaattgat caaacggttc
     2521 gggggaagcg gccgcgcgat cccgatcctt tatggcggtt cagtgaaccc caagaacgcc
     2581 aaggaaa
//
LOCUS       DASWPU010000338         4961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_82492,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000338 DASWPU010000000
VERSION     DASWPU010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82492"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VGC27_07225"
     CDS             <1..226
                     /locus_tag="VGC27_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282396.1"
                     /translation="RRLGNFFLDLLGFERQTLNTGWQAEVVLLMQDGQVAYKLMSGRP
                     RIEDTQDTIRPLGPLQDISNSAFNAASRFL"
     gene            complement(441..986)
                     /gene="infC"
                     /locus_tag="VGC27_07230"
     CDS             complement(441..986)
                     /gene="infC"
                     /locus_tag="VGC27_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY0282397.1"
                     /translation="MVPRRFASQAPAPAKDGPRVNEEIFARTILLIGDDGHKYGEIGI
                     DEGRAIAEEKGFDLVEVSPEAKPPVVKLMDYGKYKYEQQKKAAEARKKQKVIEIKEIK
                     MRPTIDDHDYDVKMKAMKRFFDDGDKVKVTLRFRGREMAHQHLGIELLNRIQKDTEPF
                     AKMEQWPRIEGRQMMMVLAPR"
     gene            complement(1152..3137)
                     /gene="thrS"
                     /locus_tag="VGC27_07235"
     CDS             complement(1152..3137)
                     /gene="thrS"
                     /locus_tag="VGC27_07235"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY0282398.1"
                     /translation="MSAPTAASPIAITLPDGKTMTFARGVTGAEIAAAIGPGLAKAAL
                     ILEVDGAEWDLFRPIEHDAKIRIITRKDDKALELIRHDASHVLAMAVQALYPGTQVTI
                     GPAIDDGFYYDFAREEPFTPEDLPKIEAKMHEIVKADLPTRREVWPRDAAVAHFEAIG
                     EHYKAELIAAIPEGEDVSIYWHGDWHDLCRGPHFASTGKVGDAFKLTKIAGAYWRGDA
                     RNAQLQRIYGTAWRDQKELDAYLARLEEAEKRDHRRIGKEMELFTFSPDVGAGLPLWM
                     PNGMVIRQELEFLALQEERKDGYRRVASPHIAKETLYLRSRHLPYYSDTMYSPLDIDG
                     ENYYLRPMNCPHHHMIYLATRHSYRELPLRIAEYGQDYRYEASGGLSGLMRVRGFCMN
                     DAHIYCRYDQAKEEFIKVMRLHARYYDLMNIKDYYMRLSLPDLDKLDKYVDQPEKWLT
                     ALGIIREAMIESGYPFKEVKGEAAFYGPKIDFEIKSVIGTEYTISTNQLDFLATQTFG
                     LNYIGEDGGEHPVYVIHRAPLGTHERFTAFLIEHYAGAFPTWLAPVQARIIPISEKVN
                     DYAETVRRKLFEVPVVNGTAGLRVDIDLTAERMQKKIRDAQLQKIPYMLVVGEREAAE
                     GKVAVRLRSGKDLGPMPLETFIARIKGEAESRKDVAE"
     gene            complement(3412..3705)
                     /locus_tag="VGC27_07240"
     CDS             complement(3412..3705)
                     /locus_tag="VGC27_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0282399.1"
                     /translation="MDVRRQVGITLRRLRLERKISQETLAFEAKIARNYVSGIERGIE
                     NPTIVVLQRLARALGVPMAEIVSPGRPGETLLKNLPRGRNVHHRGRKSPKATR"
     gene            3799..4152
                     /locus_tag="VGC27_07245"
     CDS             3799..4152
                     /locus_tag="VGC27_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282400.1"
                     /translation="MEPAHSSGLPPELESLLALWLSKCAEGGIPRADDLKAHELFHIY
                     HHHLLGAAIPKDEGIVWWQMWEEGLATYVSKRLTPACRLRQFVACVPAVVLNEPPCPR
                     AAAARRVLYGLPHGR"
     gene            4388..>4961
                     /locus_tag="VGC27_07250"
     CDS             4388..>4961
                     /locus_tag="VGC27_07250"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0282401.1"
                     /translation="MVKVKSLFVGLWALTLFAGAAMAEPQPVLLWPDGAPGSEGKTTA
                     ETMRVEPPDDQVISNVNYPSLTPYLPEPAKATGAAVIIMPGGGYSEIWITHEGIREAQ
                     FLADRGIAAFVLKYRLPYAPGSTYKVTEHSLSDVQRAIRLVKYRAAEWRIDPARVGVM
                     GFSAGGELATLAGLKSDAGDPGAADPIDREG"
BASE COUNT          856 a         1486 c         1577 g         1042 t
ORIGIN      
        1 gcgccgcttg ggcaatttct tcctcgatct gctcggcttc gaacgccaga cgctgaatac
       61 cggctggcag gcggaagtcg ttctgctgat gcaggacggg caggtcgcct acaagctgat
      121 gtcgggccgg cctcgcatcg aggacacgca agacacaatt cggcccctgg gaccgctgca
      181 ggatatcagc aactccgcgt tcaacgccgc gtcgcggttc ctgtaaggcg ggtttcgccc
      241 ggtttgaaaa acaaaaccct catgccgaag catgagggtt ttgtttagag gcgtgggctg
      301 agttggattt ggattggatc gaacatcccc ctaattcacc atggccgggc ttgacccggc
      361 catccattgg atgggcggct tggaggccgc ccatggtgag taatattgtg gattgcgtgc
      421 caattcattc cgaaaggact ctatctcggt gccagcacca tcatcatctg gcggccttcg
      481 attcgaggcc attgttccat cttggcgaag ggttcggtgt ccttctggat gcggttgagc
      541 agttcgatgc ccagatgctg gtgcgccatc tcgcggccgc ggaagcgcag cgtcaccttc
      601 accttgtcgc cgtcgtcgaa gaagcgcttc atcgccttca tcttcacgtc gtaatcgtga
      661 tcgtcgatgg tcgggcgcat cttgatctcc ttgatctcga tgaccttctg cttcttgcgc
      721 gcctcggcgg ccttcttctg ctgttcgtat ttgtatttgc cgtagtccat cagcttgacc
      781 acgggcggct tggcctcggg cgagacttcg accaggtcga agcctttttc ctccgcgatg
      841 gcgcggccct cgtcgatgcc gatttcgccg tatttatggc cgtcgtcgcc gatcagcagg
      901 atcgtgcggg cgaagatttc ctcgttgacg cgggggccgt ccttggcggg tgccggcgcc
      961 tgcgatgcga aacgtcttgg gactatgact ctctcctctt gcttggctca cgcgcagacg
     1021 ctgtttcgcg aggaccgtcc tgcctcgcga aatgtgtttc ggcttatggg cgcagggcga
     1081 atcctccaga gcgggccgca cagggccgct gaacggcggt agaataggaa taacggcaca
     1141 cgacgcaagg gctattccgc cacatctttg cgtgattccg cctcgccctt gatcctggca
     1201 atgaaggttt cgagcggcat ggggcccaaa tccttgcccg aacgcaggcg gacggccact
     1261 ttgccctcgg cggcctcgcg ctcgccgacg accagcatgt agggaatttt ctgcaactgg
     1321 gcgtcgcgga tcttcttctg catgcgctcg gcggtgagat cgatgtccac gcgcaggccg
     1381 gccgtgccgt tgaccaccgg cacttcgaac agcttgcggc gcaccgtttc ggcgtaatcg
     1441 ttgaccttct cgctgatcgg gatgatgcgg gcctggaccg gcgccagcca ggtcgggaac
     1501 gcgccggcgt aatgctcgat caggaaggcg gtgaagcgct catgtgtgcc cagcggtgcg
     1561 cgatggatga catagaccgg atgttcgccg ccgtcctccc cgatatagtt gaggccgaag
     1621 gtctgggtcg ccaggaagtc gagctggttg gtcgagatgg tgtattcggt gccgatcacg
     1681 ctcttgatct cgaagtcgat cttggggccg tagaaggcgg cttcgccctt gacctccttg
     1741 aagggatagc cggactcgat catcgcctcg cggatgatgc ccagcgccgt cagccacttt
     1801 tccggctgat cgacatattt gtcgagcttg tcgaggtcgg gcagcgacag gcgcatgtaa
     1861 tagtccttga tgttcatcag gtcgtaatag cgcgcgtgca ggcgcatcac cttgatgaac
     1921 tcttccttgg cctggtcgta gcggcaatag atgtgggcgt cgttcatgca gaagccgcgc
     1981 acccgcatca gccccgacaa gccgcccgac gcttcgtagc ggtaatcctg gccgtattcg
     2041 gcgatcctga gcggcagctc gcgataggaa tggcgcgtcg ccagatagat catgtgatgg
     2101 tgcgggcagt tcatcggccg caggtaataa ttctcgccgt cgatatcgag cggcgaatac
     2161 atggtgtcgc tgtaataggg caggtggcgc gagcgcaggt acagcgtttc cttggcgatg
     2221 tgcggcgagg ccacgcggcg atagccgtcc ttgcgctctt cctgcaaagc gaggaattcc
     2281 agttcttggc ggatcaccat gccgttgggc atccacagcg gcaggccggc gcccacatcc
     2341 ggcgagaagg tgaacagctc catctccttg ccgatgcggc gatggtcgcg cttctcggcc
     2401 tcttccaggc gcgcgagata agcgtcgagt tccttctgat cgcgccaagc agtgccgtag
     2461 atgcgctgca actgcgcgtt cctggcgtcg ccgcgccaat aggcgccggc gatcttggtc
     2521 agcttgaagg cgtcgccgac cttgccggtg gaggcgaaat gcggcccgcg gcagagatcg
     2581 tgccaatcgc cgtgccaata gatcgaaacg tcttcgcctt cgggaatggc ggcgatcagt
     2641 tcggccttgt aatgctcgcc gatggcttcg aaatgcgcca ccgccgcatc acgcggccac
     2701 acttcgcggc gcgtcggcaa gtccgccttg acgatctcat gcatcttggc ttcgatcttg
     2761 ggcaggtctt cgggcgtgaa cggttcttcg cgggcgaagt cgtaatagaa gccgtcgtcg
     2821 atggcggggc cgatggtcac ctgggtgccg ggataaagcg cctgcaccgc catggcgagg
     2881 acatgcgagg cgtcgtggcg gatcagttcc agcgccttgt cgtctttcct ggtgatgatg
     2941 cggattttgg cgtcgtgctc gatggggcgg aacaggtccc attccgcgcc gtccacctcc
     3001 aggatcagcg ccgccttggc caggccgggg ccgatggcgg ccgcgatctc ggcgccggtc
     3061 acgccgcgcg cgaacgtcat cgtcttgccg tcgggcaagg tgatggcgat gggcgaggcg
     3121 gctgtgggcg cggacactgg acttctcctt aagcgcaacc cggcgaagtg tgcaacggtt
     3181 cgccgtaagg ttgcgcgacc ataagactgc gaaatgcgcc cggaccatct tccaggggcc
     3241 ggttgcaagt caagcgaaga acgggtaggg cgcgatccgc gcggaggcgc ggcttggtct
     3301 ttctgttggc gcgcgccgca gccggcgcaa attccttttg attttcgcgc aatctgacgg
     3361 caaaccgcgt tcatacgctg gcgctatgaa ccactttgcc ggattgcgct tctagcgcgt
     3421 cgccttgggc gacttgcggc cgcggtgatg gacgttacgc ccgcgcggga ggttcttcag
     3481 taatgtttcg ccgggtctgc cggggctgac gatttccgcc atcggaacgc cgagggcgcg
     3541 cgccagacgt tgcaagacca cgatggtcgg attctcgatg ccgcgctcga tcccgctcac
     3601 atagttacgg gcgatttttg cctcgaacgc cagcgtttcc tgcgaaattt tccgctcaag
     3661 gcgcaaacgg cgcaaagtga ttcccacttg ccttcgcaca tccattcgcg aagacaagac
     3721 gattgccaac tatattggga caaactatag attgcaattg gatgcaatga aacgagcgcg
     3781 cgaatggcgg aaagaccgat ggaacctgcg cattcgagcg ggctgccgcc cgaactcgaa
     3841 tccctgctgg cgctgtggct gagcaaatgc gcggagggcg gcattccgcg cgccgacgat
     3901 ctgaaggcgc acgagctctt ccacatctat caccaccacc ttctgggcgc cgcgatcccg
     3961 aaggacgaag gcatcgtctg gtggcagatg tgggaggagg ggcttgccac ttatgtcagc
     4021 aagcgcctga cacctgcttg ccgacttcga caattcgtgg cgtgtgtccc agccgtggtt
     4081 ttaaatgagc cgccctgccc ccgagctgcc gccgcgcgca gggtactata tgggctaccg
     4141 catggccgct gaactcggcc gcaaccattc gctttcctgg ctggcccacc tttcgcccgg
     4201 ctatgtgaaa caacaggcgc gtacgtttct gatggcacaa gcccgttagg gctcgaagat
     4261 atcctcgatc atgagcttga acagcttggc gatggcgaag gcgagcggca atggccggtt
     4321 tcgagtgcgc ttttgctttc gccaattcgt ggtttgtctc atactcccgg caaattcgga
     4381 gggaacgatg gtaaaggtga aatcgttgtt cgtcggttta tgggcgctga cgctgttcgc
     4441 gggggccgcg atggccgagc ctcagccggt cctgttatgg ccggacggcg cgccgggttc
     4501 ggagggcaag accacggcgg agacgatgcg cgtcgaaccg ccggacgatc aggtgatctc
     4561 caacgtcaat tacccgtcgc tcacgcccta tctgcccgag ccggcgaaag ccaccggcgc
     4621 ggcggtgatc atcatgccgg gtggcggata ctccgaaatc tggatcacgc atgagggcat
     4681 tcgcgaggca cagttcctcg ccgaccgcgg catcgccgcc tttgtgctga aatatcgtct
     4741 cccttatgcg ccgggctcga cctacaaggt cacggaacat tcgctgtccg acgtgcagcg
     4801 cgcgatccgt ctggtgaagt accgcgccgc cgaatggcgc atcgatcccg cgcgcgtcgg
     4861 cgtgatggga ttctccgccg gaggagaatt ggcgacgctg gccggcttga aatccgacgc
     4921 cggcgacccc ggcgccgccg atccgatcga ccgcgaaggt t
//
LOCUS       DASWPU010000339         3550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_2600, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000339 DASWPU010000000
VERSION     DASWPU010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2600"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..775)
                     /locus_tag="VGC27_07255"
     CDS             complement(152..775)
                     /locus_tag="VGC27_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0282402.1"
                     /translation="MTCNGRRALSLAAIFFLTAFAAPAAKAEATDPAVQQVQTFYDTL
                     LDSMKHAKELGVKGRYEKLKPVIEQTFDLPGMVRLAVGPRWTTIPAAEQQELLAAFER
                     MTAASYAGNFNGYDGEKFVVEPATTVRGTDRVVQSKLIAKDQTVPFGYRMRQSGGSWK
                     ILDIYLNGYISQLGTQRSDLGNTLATGGAPALVKKLDALSDKLLNGN"
     gene            complement(956..1879)
                     /locus_tag="VGC27_07260"
     CDS             complement(956..1879)
                     /locus_tag="VGC27_07260"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY0282403.1"
                     /translation="MSGTSGETRQDQSRHLSQAMGQAAKAVEETLERLLPQPHGLHGR
                     VHKAMRYATFAGGKRLRPFLVLQSAGLFAVNPENALRTAAAIEVLHTYSLVHDDLPCM
                     DDDALRRGRPTVHIAFDEATAVLAGDALLTVAFEILAGAETHPAAEVRCRLVARLAEA
                     AGSNGMIGGQMIDMRAPDSTFGTDEVMLLQRLKTGQLFEFACEAGAILGEAGAEHQNR
                     LRAYARDMGLVFQITDDLLDVTSTAEKTGKAVGKDKEHGKATLVSIHGVEGARAEAGK
                     IAARAVAALDAYGPEAGALRALPPFLIGRES"
     gene            2107..3033
                     /gene="ispH"
                     /locus_tag="VGC27_07265"
                     /gene_synonym="lytB"
     CDS             2107..3033
                     /gene="ispH"
                     /locus_tag="VGC27_07265"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017387043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0282404.1"
                     /translation="MRVVLAQPRGFCAGVVRAIDIVEHALAKYGQPVYVRHEIVHNRH
                     VVETLKSKGARFVEELDEVPDGAVTVFSAHGVAPSVFQVANARGLPVLDATCPLVSKV
                     HNLGKRYVADGRTLILIGHAGHPEVEGTKGQVGAPVHLVQSEADVAALDVPPETPVAY
                     VTQTTLSIDDTRGIIAALKQRFTNIVGPDITNICYATQNRQTAVRQLCRIADVIIVVG
                     AKNSSNSNRLREIGIEEGVPSYLIADGSELDPAWVAGKKTVGLTAGASAPEALVENVI
                     AALRTIDSDLEVSQMDGKKEHIEFRLPAELRA"
     gene            3093..>3550
                     /gene="hpnH"
                     /locus_tag="VGC27_07270"
     CDS             3093..>3550
                     /gene="hpnH"
                     /locus_tag="VGC27_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEY0282405.1"
                     /translation="MGIPAKQTARIGAYVLKQHLAGRKRYPLVLMLEPLFRCNLSCSG
                     CGKIDYPDDILNQRLSYEECMEAIDECGTPCVSIAGGEPLLHREMPKIVKGFLKRGKF
                     VILCTNALLLAKKIDDYRPDPNFTWSIHLDGDKAMHDKSVCLKGTYEKAVA"
BASE COUNT          641 a         1130 c         1123 g          656 t
ORIGIN      
        1 cggcacggcc agcatgggcg agctgttgat catctcgctg gcctggacgc tgatcacgat
       61 cctgttcttc atgccggcgc tgctgggacc gccgccggaa gaacgaaagg cgcgatgggg
      121 aatagtgaac ggcaaatagc ggccgtccgc gtcagttccc gttcagcagc ttgtcggaga
      181 gcgcatcgag cttcttcacc agcgcggggg cgccgcccgt cgcgagcgtg ttgcctaaat
      241 ccgaacgctg ggttccgagc tggctgatgt agccgttgag atagatgtcg aggatcttcc
      301 agcttccgcc gctctggcgc atccgatagc cgaagggaac ggtctggtcc ttggcgatca
      361 gcttggactg caccacgcgg tcggtcccgc gtaccgtcgt ggcaggctcg acgacgaatt
      421 tttcgccgtc atagccgttg aaattgccgg cgtagctggc ggcggtcatg cgctcgaagg
      481 cggcaagcag ctcctgctgc tccgccgccg gtatcgtggt ccaacgcgga cccaccgcaa
      541 gccgcaccat gccgggaaga tcgaaggtct gctcgattac cggcttcagc ttttcgtagc
      601 gccctttcac cccaagttcc ttggcgtgct tcatgctatc gagcagagtg tcgtagaatg
      661 tctgcacctg ctgcactgcg gggtccgtgg cctcggcctt cgccgcgggc gccgcaaacg
      721 ccgtcagaaa aaatatcgcc gcgagcgaaa gcgcgcgcct accgttgcat gtcatagccg
      781 gcctctagag cgttttcagg aaaagtggaa accggttttc cgtccgaaaa cgcgacaaac
      841 taagaatcta gagcatttca tcggttccgc gaaacgatga aatgctctag cacaaatgcg
      901 ccccgatcgg ggcaaaggtg cacgctattc ttgtcggaat tatgggccct cgtcatcacg
      961 attcccggcc gatgagaaac ggcggcaggg cgcgcagcgc gccggcctcc ggaccgtaag
     1021 cgtccagggc ggcgacggcc cgcgcggcga tcttcccggc ttcggcgcgc gcgccttcca
     1081 cgccgtggat ggagaccagc gtagccttgc cgtgctcctt gtccttgccg acggccttgc
     1141 cggtcttttc cgccgtgctg gtgacgtcca agaggtcgtc ggtgatctgg aaaaccaggc
     1201 ccatgtcgcg ggcataggcg cgcaggcggt tctgatgctc cgctcccgcc tcgcccagga
     1261 tcgcgccggc ctcgcaggcg aattcgaaaa gctgcccggt cttcaatctt tgcagaagca
     1321 taacctcgtc ggtgccgaaa gtgctgtccg gcgcccgcat gtcgatcatc tggccgccga
     1381 tcatgccgtt gctgccggcg gcttccgcca gccgcgccac cagccggcag cgtacctcgg
     1441 cggcaggatg ggtttccgcc ccggccagga tttcgaaggc gacggtgaga agagcgtcgc
     1501 ccgccagaac cgccgtggct tcgtcgaagg cgatgtggac ggtgggccgg ccgcgcctca
     1561 atgcgtcgtc atccatgcaa ggcagatcgt catggaccag tgaataggta tgcagcacct
     1621 cgatcgccgc ggccgtgcgc aaggcgtttt cagggttaac ggcgaacagg ccggcgcttt
     1681 gcagaaccag gaacggcctc agccgcttgc cgccggcaaa ggtggcgtag cgcatggcct
     1741 tgtggacgcg gccatgcagg ccgtgcggct gcggcagcag acgttcgagg gtttcctcta
     1801 cggccttggc ggcttgcccc atggcctgcg acaggtgccg ggattggtcc tgccgcgttt
     1861 cgcccgatgt tccgctcata atcgcgatgc ccgctggcca aaaacagtct tcctgtctat
     1921 agcaaacgcc gcccgataca aacggcggcg ctccgggtgt ttcccggcca ttcgcacggc
     1981 aagaaagtcg ttgcgcaaga cggcgtcttt cgggaaactg tccatgagtg ggctgtgggt
     2041 cgatttcccc tttcatgtta ggggtttagt acgcggatgt gaaccatgca cgatggagcg
     2101 gattcaatgc gtgtcgtcct tgcccagccg cgcggctttt gcgccggcgt cgtccgggcc
     2161 atcgacatcg tcgaacacgc cctcgcgaag tacggccagc cggtctatgt gcgccacgag
     2221 atcgtccaca accggcatgt cgtcgaaacc ttgaagagca aaggcgcgcg cttcgtcgaa
     2281 gagctggacg aggtgccgga tggcgcggtg acggtgttca gcgcccacgg cgtggcgcca
     2341 tcggtgttcc aggtggcgaa cgcccgcggc ctgccggtac tggatgcgac ctgcccgctg
     2401 gtttccaagg tccacaatct gggcaagcgt tacgttgccg acggccgcac gctgatcctc
     2461 atcggccacg ccggacaccc ggaagtcgaa ggcaccaagg gccaggtcgg cgcgccggtg
     2521 catctggtcc aatccgaggc ggatgtcgcc gcgctggacg ttccgccgga aacgcccgtc
     2581 gcctatgtga cccagaccac gctgtcgatc gacgacaccc gcggcatcat cgcggcgctg
     2641 aagcagcgct ttacaaacat cgtcggcccc gacatcacca acatctgcta cgccacgcag
     2701 aaccgccaga cggcggtgcg ccagctctgt cgcatcgccg acgtcatcat cgtggtcggc
     2761 gccaagaaca gttcgaattc caaccggctg cgcgagatcg gcatcgagga gggcgtgcct
     2821 tcctatctga tcgccgacgg cagcgaactc gatcccgcct gggtggcggg caagaagacc
     2881 gtggggctga ccgcgggcgc ctcggcgccg gaagcgctgg tcgagaacgt gatcgccgcg
     2941 ttgcggacca tcgattccga cctcgaagtc tcgcagatgg acggcaagaa ggaacatatc
     3001 gagtttcggc ttcccgccga gctcagagct tagtcctttg gggcgcccac ccgcatggtg
     3061 cgggcgggcc tctcaggatg aggagtttta ttttgggtat tccggcaaag cagacggccc
     3121 gcatcggggc ctatgtcctc aagcaacatc tggcgggcag gaagcgctat ccgctggtgc
     3181 tgatgctgga accgttgttc cgctgcaatc tctcctgctc cggctgcggc aagatcgatt
     3241 atcccgacga catcctcaac cagcgcctca gctatgagga gtgcatggaa gcgatcgacg
     3301 agtgcggtac gccctgtgtc tcgatcgccg gcggcgaacc gctgctccat cgcgagatgc
     3361 ccaagatcgt gaagggcttc ctcaagcgcg gcaaattcgt catcctgtgc accaacgcgc
     3421 tgctgctggc caagaaaatc gacgattacc ggcccgatcc gaacttcacc tggtcgatcc
     3481 acctcgacgg cgacaaggcg atgcacgaca aatcggtgtg cctgaaaggg acgtacgaaa
     3541 aggccgtcgc
//
LOCUS       DASWPU010000340         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_45902,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000340 DASWPU010000000
VERSION     DASWPU010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45902"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1156)
                     /gene="pheS"
                     /locus_tag="VGC27_07275"
     CDS             complement(80..1156)
                     /gene="pheS"
                     /locus_tag="VGC27_07275"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004979194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY0282406.1"
                     /translation="MSDIQALERQILDAIAGASDEAALEVVRVSALGKKGAVSELLKT
                     LGAMSQDERKANGPLFNGLRDRVAGALAARKAALEEESLNARLACERIDITLPPRPEA
                     AGTIHPVSQALDEVTAIFADLGFAIAEGPDVEFDDYNFTKLNIPPDHPARQMHDTFYV
                     AKQADGSRHVLRTHTSPVQVRTMLKQKPPIRVISPGRTYRVDSDATHSPMFHQFEGLV
                     IDEVTHLGHLKWVLEEFCKAFFEVECVEMRARPSFFPFTEPSVEWDLRCDRSGGQIKF
                     GQGNDWLELAGSGMVNPRVLENCGIDSARYQGYAFGGGIDRLAMLKYGIPDIRSFFES
                     DLRWLRHYGFAALDIPTLDGRLSR"
     gene            complement(1225..>1513)
                     /gene="rplT"
                     /locus_tag="VGC27_07280"
     CDS             complement(1225..>1513)
                     /gene="rplT"
                     /locus_tag="VGC27_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY0282407.1"
                     /translation="FRGRRKNTITSANAAVDHSLQYNYIGRKLKKRNFRALWIQRINA
                     ACREHGITYSRFISGLLKAGIEIDRKVLADLAVKEPVAFKALVDQAGGAAA"
BASE COUNT          265 a          448 c          504 g          296 t
ORIGIN      
        1 gataaaggac tcacgcggag gcgcggaggg gcggtgaatc tccgtgtctc cgcgtgaatc
       61 ggacaatagt cgcgtgcact catcggctca gccgtccatc cagcgtggga atgtcgaggg
      121 ccgcgaagcc gtaatggcga agccagcgca gatcggattc gaagaaggag cggatatcgg
      181 gaatgccgta tttgagcatg gcgagccggt cgatgccgcc gccgaaggcg tagccctggt
      241 agcgcgcgga atcgatgccg caattctcca gtacgcgcgg gtttaccatg cctgagccgg
      301 ccagctccag ccagtcgtta ccttggccga atttgatctg gccgcccgaa cggtcgcagc
      361 gcagatccca ttccacgctc ggttcggtga aaggaaagaa gctgggccgc gcccgcattt
      421 ccacgcactc gacttcgaag aacgctttgc agaattcctc cagcacccat ttgaggtggc
      481 ctagatgggt cacttcgtcg atcactaatc cttcgaactg gtggaacatc ggcgaatggg
      541 tggcgtcgga atccacccga taggtgcgcc ccggcgaaat gacgcggatc ggcggcttct
      601 gtttcagcat ggtgcgcacc tgcaccgggg aggtgtgggt gcgcagcaca tggcgcgagc
      661 cgtcggcctg tttggccaca tagaaggtgt cgtgcatctg ccgcgccgga tgatcgggcg
      721 gaatgttgag cttggtgaaa ttgtaatcgt cgaattcgac atccgggcct tcggcgatgg
      781 cgaagccgag gtcggcgaag atcgccgtca cttcgtcgag cgcctggctg accggatgaa
      841 tcgtgccggc ggcttccggg cgcgggggca gggtgatgtc gatgcgttcg caggccagcc
      901 gcgcgttgag gctttcttct tccagcgccg ctttgcgtgc ggcaagcgcg cccgccaccc
      961 ggtcgcgcag accgttgaat agcgggccgt tcgccttgcg ctcgtcctgg ctcatggcgc
     1021 cgagcgtctt cagcagctcg ctgacggcgc ccttcttgcc gagcgcggag acgcgcacga
     1081 cttccagcgc ggcctcgtcg gaggcgcccg caatggcgtc gaggatttgc cgttcgaggg
     1141 cttgaatgtc gctcatggaa aacccagacc gcttgaatca cggactttgg cgattgccaa
     1201 ggcccatcgg ctccgaggtc tgatttaggc cgctgcgccg cctgcctgat ccaccagagc
     1261 cttgaaggct acgggctcct tgacggcgag atcggcaagg accttgcggt cgatctcgat
     1321 cccggccttg agaagcccgc tgataaatcg gctgtaggtg atgccgtgct cgcggcacgc
     1381 cgcgttgatg cgctggatcc aaagcgcgcg gaaattgcgc ttcttcagct tgcggccgat
     1441 gtaattgtac tgcagcgaat gatcgaccgc ggcattggcc gaggtgatcg tgttcttgcg
     1501 gcgtccgcgg aag
//
LOCUS       DASWPU010000341        10219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_8770, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000341 DASWPU010000000
VERSION     DASWPU010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10219
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8770"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1781
                     /locus_tag="VGC27_07285"
     CDS             <1..1781
                     /locus_tag="VGC27_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0282408.1"
                     /translation="SMTVSLAAVFIPVLFMGGIVGRLLHEFAVTIVLAIVFSGIVSVT
                     LTPMLCSRMLKGHDTSSHGAFYRWSERAFQDVQNFYERTLRWSLEHGRTILLVFAGSL
                     VATVVLFLVMPQDFLPSEDTGMINGSTEAANGTSYPQMVKYQQAVARIINADPSVQGA
                     MSSVNDSNTGRVLLHLKDRSERSLSADQIIAQLRGKLASVPGISVVMQNPPSIRIGGR
                     QSRAQYQYTLQDLDMDELQDATEKLTAALQAERGFQDVTNDLRLTTPTVTVGIDRDRA
                     ASLGVTPQQIETALGTAFGGQQISQIYTSTDDYQVIMELLPQYQTDASSLSRLYITAA
                     DGTLVPLTAVTKIGSSTTPLSINHSGQLPAATVSFNLDSGMALSDAVSTINRVQRQIA
                     LPGGVVGSFQGTAAAFQSSMGNITVLLIIAIIVVYIVLGILYESFVHPLTILSGLPSA
                     AVGALLTLALFGMPLSLYAFVGMIMLIGIVKKNAIMMIDFALVRQRNEGMPAPAAIFD
                     AALVRFRPIMMTTMAALMGTLPIAIGLGAGSEARRPLGLAVVGGLLLSQMLTLYITPV
                     IYVYLDRLGARVGRRGAVAAAASGVS"
     gene            complement(1774..2085)
                     /locus_tag="VGC27_07290"
     CDS             complement(1774..2085)
                     /locus_tag="VGC27_07290"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEY0282409.1"
                     /translation="MTDAPTGNGNNAQTAARLPFPVVRLLYAIGFAVVAWFVFWIVLL
                     LAVLQFVIVAIQGKQNGELKGLSLNLVQYLWELLAFIVFARDEQPFPFGPFPHATATP
                     N"
     gene            complement(2158..2859)
                     /locus_tag="VGC27_07295"
     CDS             complement(2158..2859)
                     /locus_tag="VGC27_07295"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY0282410.1"
                     /translation="MAFLQSQPPREPFLQAPASVLWLIGALALAHIARMAIPAALSDE
                     ILAQYAFIPAHYSGGLDGGGFFGLAAPFVSHMFLHADFVHLGVNCIWLLAFGPIVARR
                     YGTGLFLLFFAICGVAGAAAYLAFDWGSFVGVIGASGAISGLMAAGIRMYPWPGAPLA
                     RPMAPIFSSPILLFTAFWLATNLLFGLLGIGANGEVTEIAWQAHLGGYFAGLVLAGGF
                     EALRRRHTAKRPGVG"
     gene            3157..3585
                     /gene="hfaA"
                     /locus_tag="VGC27_07300"
     CDS             3157..3585
                     /gene="hfaA"
                     /locus_tag="VGC27_07300"
                     /inference="COORDINATES: protein motif:HMM:NF037934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchoring protein HfaA"
                     /protein_id="HEY0282411.1"
                     /translation="MPFDFKQLLLASLVVLGAIALEGVAHANDYSSSASYNSAYGLSP
                     GAENQAINPSLRDANGNLTVVNGQFTSSAFSRQTGVQSMGTISSGTLSSFGTGAAYGN
                     ASAIGNSLNVVTIGNNNTVVVNSSQTNNGNQNASVSLNGQ"
     gene            3605..4642
                     /gene="hfaB"
                     /locus_tag="VGC27_07305"
     CDS             3605..4642
                     /gene="hfaB"
                     /locus_tag="VGC27_07305"
                     /inference="COORDINATES: protein motif:HMM:NF037935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchoring protein HfaB"
                     /protein_id="HEY0282412.1"
                     /translation="MTPRGRKYLLRSLAIVIAATAFGGCISPIPTASGRYTAPIGGAP
                     VISNETPYSSALRCMGRYTAGRPLRVAVGQIADYTGKFESDNSGRKLTQGAALMAMSA
                     LSKAGVPLVERFDTSVAEMELKYANNKLIGDDAPGAPGDFRKIMAGSIPGSNYYLVGG
                     ITELNFNIRSEGASANGGGVATNAVKGTIGASMYVMNVGLDLRLVDTNTLEVVDVISY
                     QKQIIGRQISAGVFDFLGQTFFDASVGESALEPVQLAVRSVIERAMLEMMSRLYHTPQ
                     IACAANLGTGSDPLADASDRRPTPDPAQSRHTTTAMNQEKNNDTSRQDPYRWYSDGDV
                     ASSPGLRGRYQ"
     gene            4596..5228
                     /locus_tag="VGC27_07310"
     CDS             4596..5228
                     /locus_tag="VGC27_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282413.1"
                     /translation="MAMLPLAQAFAAGTSDQSGSSQILNGQVALHTSISDLHTVIDNV
                     GSDVGVQSVAAGNVVDITTMNDTHVTNNQYVSSVDISSDLGARVTNVGGNVGIQGQAV
                     CNSASVLTDPAITAISNTQVCEAIDPASNAYVDAANISGSFSLANSAIGNSLQADTNA
                     ANMPITSTQVNHSNVDAVTTANVFNVAGTVSVTSAAIGNNAQIVHYSTGN"
     gene            complement(5650..5940)
                     /locus_tag="VGC27_07315"
     CDS             complement(5650..5940)
                     /locus_tag="VGC27_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY0282414.1"
                     /translation="MQKLYYVYMLASQRNGTLYTGVTNDIGRRIWEHKSDLVEGFTKK
                     YGVHILVWYEVYEDINVAIAREKQIKGWNRAWKIRLIEKENSGWNDLYEKLG"
     gene            complement(6006..7172)
                     /locus_tag="VGC27_07320"
     CDS             complement(6006..7172)
                     /locus_tag="VGC27_07320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0282415.1"
                     /translation="MDIALNEDQRAIEDGVAKICARFGDDYWTECDTAPRFPHEFHKA
                     MAEAGFLGITMPEELGGSGLGVTEAAVMMHTVTQYGGGMAAASAIHINLFGPHPIVVH
                     GTGEQKKRWLEPLIAGEHKVAFGVTEPNAGLDTTSIETFARRTAGGYSIRGRKMWTTT
                     AQVADKILLLTRTTPKAECKRPTEGMTLFYTDLDRSKIEVRRIAKMGRSAVDSNALFI
                     DDLAVPEADRIGEEGKGFRYLLDSLNPERILFGVEAIGLGRDALRRASQYAKERRVFG
                     RAIGQNQGIQHPLAENWAYLESAFWTCMRAAWLYDNKQPCGAEANAAKFLGARAAFEA
                     ATQAVMTHGGMGYAKEYQVERLFREAVLTRIIPVTEQLILSFIGENVLDLPKSY"
     gene            7446..9227
                     /locus_tag="VGC27_07325"
     CDS             7446..9227
                     /locus_tag="VGC27_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0282416.1"
                     /translation="MAPTDIANPNYFHKVVDCQWACPAHTPVPEYIRLIAAGRYSDAY
                     MINWYSNVFPGVLGRTCDRPCEPACRRGRVEKEPVAICRLKRVAADSKDDISERLPKP
                     ASVKNGKRIALIGAGPASLTVARDLLPLGYHCVIYDSDLRAGGMMRTQIPRFRLPEHV
                     LDEECGYILALEPETKFGHKVESLKAVLAEGYDAVFVGCGAPRGRDIDIPGRKEAAEH
                     IHIGIDWLASVSFGHTTRIGKRVIVLGGGNTAMDCCRTSRRLGGEDVQVVVRSGFGEM
                     KASPWEKEDAMGEGIPIHNFLVPTEFIHANAALTGVIFEKVKAEYDAKGRRKLIPTGE
                     APVHMACDDVLVAVGQENAFPWIERDIGVDFDQWNMPKIDPVTMQSTHPQVFFGGDAA
                     FGPKNIITAVAHGHEAALSIDLFCHGQDVSARPGPLVNILGQKMGIHEWSYDNDVSPD
                     LRFKVPHAPKEMTLKDVNMEVELGFNRELAFKEAQRCLNCDIETVFEEALCIECDACV
                     DICPMDCITFTPNAPEPVLRTLLTAPAKNLTQALYVSAGLNTGNVMVKDEDVCLHCGL
                     CAERCPTGAWDMKKFYLEIAQAPACSR"
     gene            9215..>10219
                     /locus_tag="VGC27_07330"
     CDS             9215..>10219
                     /locus_tag="VGC27_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY0282417.1"
                     /translation="MQPISATNDFVVKFANVNGSGSASANELFARAILRMGVPVGSRN
                     IFPSNIQGLPTWYEVRVTSEGWQGRRGGVDLMVAMNPQTWDKDVASIDPGGYLLYDST
                     KPFPAATFRKDVTLLPVPLTELCNRAFSNPRQRQLFKNIGYIGALAFLLGIEASAVEW
                     LIAEQFEGKQKLIAPNLQAFNLGYDYARKNLKGACALKLASTDKVGDRLFISGNEAAA
                     LGCVWGGATVCAWYPITPSTSLPEYFAKHCARFRIDPISKKNRFAIVQAEDEIAAIGM
                     VLGAGWNGARSFTATSGPGISLMQEFFSFAYYAEVPAVVFDIQRGGPSTGMPTRTQQS
                     D"
BASE COUNT         2046 a         3282 c         3017 g         1874 t
ORIGIN      
        1 tgtcgatgac ggtctccctc gccgcggtgt tcattccggt cctgttcatg ggaggcatcg
       61 tcggccgtct cctgcacgaa ttcgcggtga cgatcgtact ggcgatcgtc ttctccggca
      121 tcgtgtcggt gacgctgacg ccgatgctgt gcagccgcat gctcaagggg catgacactt
      181 cgagccatgg cgcgttctac cgctggagcg aacgcgcgtt ccaggatgta cagaactttt
      241 acgagcgcac cttgcgctgg agtctggaac acggccgcac gatattgctc gtcttcgccg
      301 gaagcctggt tgccaccgtc gtgctgttcc tcgtgatgcc gcaggatttc ctgcccagcg
      361 aagacaccgg catgatcaac ggcagcacgg aggccgcgaa cggaacatcg tacccccaga
      421 tggtgaagta ccagcaggcg gtcgcgcgca tcatcaacgc cgaccccagc gtccagggcg
      481 cgatgtcgtc ggtgaacgat tcgaacaccg gcagggttct cctccatttg aaggatcgtt
      541 ccgaacgctc cctttcggcc gaccagatca tcgcccaact gcggggcaag ctcgcgagcg
      601 ttccgggcat cagcgtcgtc atgcagaacc cgcccagcat ccgtatcggc ggcaggcagt
      661 cgcgggcgca gtaccaatat accctgcaag acctcgatat ggacgagttg caggacgcaa
      721 cggagaagct gacggcggcc ctgcaagccg agcggggctt ccaggacgtg accaacgatc
      781 tccggctgac gacgccgacg gtcaccgtgg gcatcgaccg ggaccgcgcc gcctcgctcg
      841 gggtcacgcc gcaacagatc gaaacggcgc tggggacggc attcggcggg caacagatct
      901 ctcagatcta cacttccacc gacgattacc aagtaatcat ggaactgctg ccgcaatatc
      961 agaccgacgc atcttcgctc tcgcgtttat acatcaccgc ggccgacgga acgctggtgc
     1021 cgctgacggc ggtgacgaaa atcggttcga gcaccacgcc gctcagcatc aatcattcgg
     1081 gacagttacc cgccgcgacc gtctcgttca acttggacag cggcatggcc ctcagcgacg
     1141 cggtttccac gatcaacaga gtgcagcgtc aaatcgctct tccgggcggc gtcgtcggca
     1201 gcttccaagg cacggcggcg gccttccaaa gctcgatggg caatatcacc gtactgctca
     1261 tcatcgccat catcgtcgtc tatatcgtgc tgggcattct atacgagagc ttcgtgcacc
     1321 cgctgacgat cctgtccggt ctgccttccg ccgccgtcgg ggcgttgctg accctggcgc
     1381 tgttcggcat gccgctgagc ctgtacgcct tcgtgggcat gatcatgctg atcggaatcg
     1441 tgaagaagaa cgcgattatg atgatcgact tcgcgctggt ccgccaacgc aacgaaggca
     1501 tgcccgcccc ggccgccatc ttcgatgccg cgctggtgcg ctttcgcccg atcatgatga
     1561 cgacgatggc cgcgttgatg ggcacgcttc ccatcgccat cggcctgggc gccggttcgg
     1621 aggcgcgccg cccgctcggt cttgccgtcg tcggcggcct gctgctttct cagatgctga
     1681 cgctttacat cacgccggtg atctatgtgt atctcgatcg cctcggcgcg cgggtaggca
     1741 ggcgcggcgc cgtcgccgcg gcggcttccg gtgtcagtta ggcgtcgcgg tcgcgtgcgg
     1801 gaacggaccg aacggaaacg gctgctcgtc gcgggcaaag acgatgaaag ccagcagctc
     1861 ccagaggtac tgtacgagat tcaaagacag gcccttgagt tcgccgttct gcttgccctg
     1921 gatcgcgacg atcacgaact gcaacacggc gagcagcaga acgatccaga acacgaacca
     1981 tgccaccacg gcaaagccta tggcatacag aagtctcacg actgggaacg gcagtcgggc
     2041 ggcggtctgg gcgttgtttc cgttgcccgt gggagcgtcg gtcatagtga ataccctcca
     2101 agatgcgagg cgacaaacgc cgccaaccct tcttacttag ggtggcaggg gggatgttca
     2161 acccacgccc ggcctctttg cggtatggcg tctgcgcaag gcctcaaatc ctccggccaa
     2221 gaccagaccg gcaaaatagc cgccgagatg cgcctgccag gcaatctctg tgacttcgcc
     2281 attggctccg atccccaaaa ggccgaacag caggttcgtg gcgagccaaa acgcggtgaa
     2341 aagcaggatc ggcgacgaga aaatcggcgc catcggccga gccagaggcg ctcccggcca
     2401 aggatacatc cggatgccgg cggccatgag cccggatatg gcgcccgacg ccccgatgac
     2461 acccacgaac gagccccaat cgaaggcaag ataggccgcc gccccagcca cgccgcaaat
     2521 ggcgaaaaac agaagaaaga gccccgtccc gtagcggcgc gccacgatcg gcccgaaagc
     2581 cagcaaccat atgcagttca cacccaggtg gacgaaatct gcgtgcagga acatgtggct
     2641 gacgaaaggc gcggccaggc cgaaaaatcc tccaccatcc aagccgccgc tgtaatgggc
     2701 cggaatgaag gcatattgtg ccaatatctc gtccgacaac gcagccggta tggccatcct
     2761 cgcaatatgc gcaagggcaa gcgccccgat cagccacagg acggacgccg gcgcctgcag
     2821 aaacggttcg cgcgggggtt gggactggag gaatgccatg tcagtgattt ccgctgctta
     2881 gttgaacgcg gctcgtcgcg aatccgcttg tctcataata aaacgccctc cacggccggc
     2941 gacgatcccc cctacaccat tatcgctgcc gacgatgaac ttacctccgt gggtctgccc
     3001 tttatcgctc gccggcatat cgagtaaagc cggtttgggc cgcaattgca cgttaacggc
     3061 gtccgtgccg gtttggcacg ttgtcatgcc ttaatcccca ttaagcgttg gcatgcccat
     3121 tgctccagga cgttcgagac cgttgggacg cctgacatgc cgttcgattt taagcagctt
     3181 ctgcttgcca gcctcgtcgt tctgggcgcc atcgccttgg aaggcgtggc ccacgcaaac
     3241 gactatagca gctcggcgag ctacaacagt gcttacggcc tgtcaccggg cgcggaaaac
     3301 caggcaatca acccgagcct gcgcgacgca aacggcaatc tgaccgtcgt caacggccag
     3361 ttcaccagct ccgccttcag cagacagacc ggcgtgcaaa gcatgggcac gatcagctcc
     3421 ggcacgctct cttcgttcgg caccggcgcg gcgtacggca atgcctcggc catcggcaat
     3481 tcgctcaacg tcgtgacgat cggcaacaac aataccgtcg tcgtgaattc cagccagacc
     3541 aataacggaa atcagaatgc gtccgtcagc ctcaacggac agtaagagaa gggaacttca
     3601 tcccatgacc ccgcgcggcc gcaaatatct tctcagatcg ctggcgatcg tcatcgcagc
     3661 cacggccttt ggcggatgca tcagtccgat cccgacggcc agcgggcgct acaccgcgcc
     3721 catcggcggc gcgcccgtca tcagcaacga gacgccctat tcgtccgctc tgcgctgcat
     3781 gggccgctac accgcaggca ggcctttgcg tgtcgccgtg ggacagatcg ccgactatac
     3841 gggcaagttc gagagcgaca attccgggcg caagctcacc cagggcgccg ccctgatggc
     3901 aatgagcgcg ctctccaagg ccggcgtgcc gctggtcgaa cgcttcgata cctccgtcgc
     3961 cgagatggaa ctcaaatacg ccaacaacaa gctgatcggc gacgacgctc cgggcgcgcc
     4021 cggcgatttc cgtaagatca tggccggttc gattcccggc tccaattact atctggtcgg
     4081 cggcatcacg gaactcaact tcaatatccg ttcggaaggc gccagcgcca acggcggcgg
     4141 tgtcgccacc aatgccgtca agggtacgat cggcgcctcg atgtatgtga tgaacgtcgg
     4201 tctcgatctt cgtctggtgg acaccaacac gctcgaagtc gtcgacgtga tctcgtacca
     4261 gaagcagatc atcggacggc agatttccgc cggcgtattc gacttcctgg gccagacctt
     4321 cttcgacgcc agcgtcgggg aaagcgctct ggagcccgtc cagctcgcgg tgcgttcggt
     4381 gatcgagcgc gcgatgctgg aaatgatgtc gcgcctctac cacacgcctc agatcgcctg
     4441 cgccgccaac cttggcaccg gcagcgatcc cttggccgac gcatccgacc gccggcccac
     4501 gcccgatccc gcccaatcaa gacatacgac aaccgccatg aaccaggaga agaacaatga
     4561 cacgtctcgc caagatcctt atcgctggta cagcgatggc gatgttgcct ctagcccagg
     4621 ccttcgcggc cggtaccagt gaccagtctg gcagcagtca gattctcaac ggtcaggtag
     4681 cgctgcacac gtcgatttcg gacctgcaca cggtgatcga caatgtcggt tccgacgtgg
     4741 gcgtccaatc ggtggccgcg ggcaacgtgg tcgacattac gaccatgaac gacacgcacg
     4801 tgaccaacaa tcagtatgtg tcgagcgtgg acatcagttc cgacctcggc gcacgcgtca
     4861 cgaacgtggg cggcaacgtc ggaatccaag gccaggcggt ttgcaactcg gccagcgttt
     4921 taaccgatcc tgccatcacg gcgatcagca acacgcaggt gtgcgaagcg atcgatccgg
     4981 cgtcgaatgc ctatgtcgat gcggccaaca ttagcggcag ctttagcctg gccaattcgg
     5041 cgatcggcaa ctcgctccag gcggatacca acgccgccaa catgccgatc acatcgaccc
     5101 aggttaatca ctccaacgtc gacgccgtga ccaccgccaa cgtcttcaat gtcgccggca
     5161 cggtcagcgt cacttcggcc gccatcggca acaacgcgca aatcgtgcac tactcgacag
     5221 gcaactgaac gacacgcgtc gcgaaagaca tcccttgggg caaaatgggg gtgtcgggga
     5281 agaagaggaa gggcagtcaa aaagcctttc ctcttttcgc atctgagcca agtctttgta
     5341 ttttcttaaa tatccccgga aggttgtttt cgactttttt catgggggtg gggggcaccc
     5401 actctccctc acttttggca ggcgcgtttc cgcctagacc acgccgcttt agaaatgggg
     5461 tgcagaagca tgccgttcaa gaccgcgcgg ctatccatgc cggtgccggt tcgctgccaa
     5521 cggccgatct ggctctcgcg ggcacgcaag ataaacgtgt gcctacaaca ccaaatctcg
     5581 catcattccc ggccgagcga ccgaaggaag tgaggggaag ggaacccagg cgccgcaacc
     5641 aagcatgatc tatccgagct tctcatagag atcgttccag ccggaattct ctttttcgat
     5701 aagacggatt ttccaggcac ggttccagcc cttgatctgc ttctcacgcg cgatggctac
     5761 gttaatgtcc tcgtacacct cataccaaac gaggatgtgc acgccatatt tcttggtgaa
     5821 cccttcaacc aaatcgcttt tgtgttccca gatgcgccta ccgatgtcat tcgttacgcc
     5881 ggtgtatagc gttccgtttc gctggcttgc cagcatgtag acgtagtaga gcttctgcac
     5941 ctgggtcccc ttccctcgcg cttcgcgctc gccggggatg acaacctaat attgaaacag
     6001 cgcttctagt atgacttcgg caaatccagc acgttctcgc cgatgaagga gagaatgagt
     6061 tgctcggtca ccgggatgat gcgcgtcaga acggcttcgc ggaacagccg ttccacctga
     6121 tattccttgg cgtagcccat gccgccatgc gtcatcaccg cctgggtcgc cgcctcgaac
     6181 gccgcacgcg cgccgaggaa cttcgccgcg ttggcttccg cgccgcaagg ctgcttgttg
     6241 tcgtaaagcc aggccgcgcg catgcaggtc cagaacgcgc tttccagata agcccaattc
     6301 tcggccagcg gatgctggat gccctggttc tggccgatgg cgcggccgaa gacgcgccgt
     6361 tcttttgcat attggctggc gcgcctgagc gcatcgcggc cgagcccgat ggcttccacg
     6421 ccgaacagga tgcgttccgg attcaaactg tcgagcagat agcggaagcc cttgccttcc
     6481 tcgccgatgc ggtctgcttc cggcaccgcc agatcgtcga tgaacagcgc gttggaatcg
     6541 accgccgagc ggcccatctt ggcgatgcgg cgaacctcga tcttggaacg gtcgagatcg
     6601 gtgtagaaca gagtcatccc ctcggtcggc cgcttgcact ccgccttcgg cgtggtgcgc
     6661 gtcagaagca gaattttgtc ggcgacctgc gcggtggtgg tccacatctt tcgcccacga
     6721 atggaatagc cgccagcggt gcgccgcgcg aaggtttcga tggaagtcgt atcgaggccg
     6781 gcatttggct cggtgacgcc gaatgcgacc ttgtgctcgc cagcgatgag aggctcaagc
     6841 cagcgcttct tctgctcgcc ggtgccgtgc acgacgatgg gatgcggtcc gaacaggttg
     6901 atgtggatgg ccgatgccgc cgccatgccg ccgccgtact gcgtcaccgt atgcatcatc
     6961 acggcggcct cggtgacgcc cagccccgaa ccgccaagct cttccggcat ggtgatgccg
     7021 agaaatcccg cctcggccat ggccttgtgg aactcgtggg gaaagcgcgg agcggtgtcg
     7081 cactcggtcc aataatcgtc gccgaagcgg gcgcaaatct tggcgacgcc gtcttcgatg
     7141 gcgcgctggt cctcgttaag cgcaatatcc atgtttcaac tctggtttat tgattctttt
     7201 acttcaatgg gttacgcgaa accttccgta ttgcaatgca ggatcggcaa agctcttgct
     7261 acaaattatt tggcgaaaca aggggaaacg ccccaccgaa gacgctatat aaaggcgttc
     7321 ccgcaaggga cgagggccag cacaggcggc aagccgcgcg tattgccggg agaataacca
     7381 aaacaatgag ttccgtatcg gctggagggc gcaagacatc gcccgggccg gcgcgcagtg
     7441 cagggatggc gccgaccgat atcgcaaacc cgaattactt ccataaagtc gtcgactgcc
     7501 aatgggcttg tccggcgcac acgccggtgc ccgaatatat ccgcctgatc gcggccgggc
     7561 gctattccga cgcctacatg atcaattggt actccaacgt ctttcccggc gtgctcggac
     7621 gcacctgcga ccggccttgc gagccggcct gccggcgcgg gcgggtggag aaagaacccg
     7681 tcgccatctg ccggctgaag cgcgtggcgg cggattccaa ggacgacatc agcgagcggc
     7741 tgcccaagcc cgcttccgtc aagaacggca agcgcatcgc gctgatcggc gcgggccccg
     7801 cttcgctgac ggtggcgcgc gatctgctgc cgctcggcta tcactgcgtg atctacgatt
     7861 ccgatttgcg cgccggcggg atgatgcgca cgcaaatccc gagattccgc ctgcccgagc
     7921 atgtgctcga cgaagaatgc ggctacatcc tggcgctcga acccgaaacc aagttcggcc
     7981 acaaggtgga atcgctgaaa gcggttctgg cggaaggtta tgacgcggtt ttcgtgggct
     8041 gtggcgcgcc gcgcggacgc gacatcgaca ttcccggccg caaggaagcc gcagagcaca
     8101 tccatatcgg tatcgactgg ctggcctcgg tttcgttcgg ccataccacc aggatcggca
     8161 agcgcgtcat cgtgctgggc ggcggcaaca cggcgatgga ctgctgccgc acctcgcgcc
     8221 gcctgggcgg cgaagatgtt caggtcgtgg tgcgctccgg cttcggcgag atgaaggcgt
     8281 cgccctggga aaaagaagac gcgatgggcg aaggcatccc catccacaat ttcctggtac
     8341 ccacggagtt catccacgcc aacgcagcgt tgaccggcgt catcttcgaa aaagtgaagg
     8401 ccgagtacga cgcgaaaggg cggcgcaagc tcatccccac cggcgaggcg ccggtgcata
     8461 tggcgtgcga tgacgtgctg gtcgcggtcg gtcaggaaaa cgccttcccc tggatcgagc
     8521 gcgacatcgg cgtggacttc gaccaatgga acatgcccaa gatcgatccc gtcaccatgc
     8581 aatcgaccca tccacaggtg ttcttcggcg gcgacgcggc cttcgggccc aagaacatca
     8641 tcaccgccgt ggcgcacggc cacgaagcag cactttccat cgatctcttc tgccacggcc
     8701 aagatgtgtc ggcgcggccg ggcccactgg taaatattct cggccagaag atgggcatcc
     8761 acgaatggtc ctacgacaac gacgtgtcgc cggatctgcg gttcaaagtg ccgcacgcgc
     8821 ccaaggagat gacgctcaag gacgtcaaca tggaagtgga actgggcttc aaccgggaac
     8881 tcgccttcaa ggaggcgcag cgctgcctta attgcgacat cgagacggtg ttcgaggagg
     8941 ctctctgcat cgaatgcgac gcctgtgtcg atatctgccc gatggactgc atcaccttca
     9001 cccccaacgc gcccgagccg gtgctgcgca cgctgctgac cgcgcccgcg aagaacctga
     9061 cgcaggctct ctatgtctcc gccgggctga acaccggcaa cgtcatggtc aaggacgaag
     9121 atgtctgcct gcattgcggg ctatgcgccg agcgctgtcc gaccggtgcc tgggacatga
     9181 agaagttcta tctggaaatc gcgcaggcac ccgcatgcag ccgataagcg ccaccaacga
     9241 tttcgtcgtc aaattcgcga acgtcaacgg ttcgggatcg gccagcgcca acgagctgtt
     9301 cgcccgcgcc attctgcgca tgggtgtgcc ggtaggttcg cgcaacatct tcccctcgaa
     9361 catccaaggc ctgccgacct ggtacgaagt gcgcgtcacc agcgaagggt ggcagggaag
     9421 gcgcggcggc gtcgatctga tggtcgcaat gaacccgcag acctgggaca aggacgtggc
     9481 cagcatcgat cctggcggct atctgctcta cgattcgacc aagcccttcc cggccgcgac
     9541 cttccgcaag gacgtgacgc tgctgccggt gccgctgacc gaactttgca accgcgcctt
     9601 cagcaacccg cgccaacgcc aattgttcaa gaacatcggc tatatcggcg cgctggcgtt
     9661 cctgctcggc atcgaagcca gcgccgtcga atggctgatc gccgaacagt tcgaaggcaa
     9721 gcagaagttg atcgcgccca atctccaggc gttcaatctc ggctacgatt atgcccgcaa
     9781 gaacctcaag ggcgcctgcg cgctgaagct cgcgagcacg gacaaggtgg gcgaccgtct
     9841 cttcatctcc ggcaacgagg cggcggcttt gggctgcgtc tggggcgggg cgacggtgtg
     9901 cgcctggtat ccgatcacac cctccacctc gctgccggaa tatttcgcca agcactgcgc
     9961 gcgcttccgc atcgatccca tcagtaagaa gaaccgcttc gccatcgtgc aggccgagga
    10021 cgaaatcgcc gccatcggca tggtgttggg cgccggctgg aacggcgcac gctccttcac
    10081 cgccaccagc ggccccggca tctcgctgat gcaggagttc ttcagcttcg cctattacgc
    10141 cgaagtgccc gccgtcgtgt tcgacatcca gcgcggcgga ccctccaccg gcatgccgac
    10201 gcgcacccag caatcggat
//
LOCUS       DASWPU010000342         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_113226,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000342 DASWPU010000000
VERSION     DASWPU010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113226"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..597
                     /gene="smpB"
                     /locus_tag="VGC27_07335"
     CDS             124..597
                     /gene="smpB"
                     /locus_tag="VGC27_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY0282418.1"
                     /translation="MAKKKDDDRKIVADNRKARFSYAIESSLEAGIALTGSEVKSLRT
                     GKASIAESYAQAKDGEIFLINANIPEYGKAARFGHEPKRTRKLLVHKREAAKLSIAIQ
                     REGMTLIPLKLYFTPKGIAKLELGIAKGKKLHDKRETEKKRDWARDKARIMRNKG"
     gene            complement(862..1503)
                     /locus_tag="VGC27_07340"
     CDS             complement(862..1503)
                     /locus_tag="VGC27_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0282419.1"
                     /translation="MSAPEPPHDCLLCPRLAQFRAANAKAYPDFFNAPVPTFGANEAR
                     LLIVGLAPGLKGANRTGRPFTGDWAGDLLFAMLLKHGLARGTYDERADDGLTLAECAI
                     TNAVRCVPPENKPTPAEIKTCRKFLVGQISAMPHLRAILALGKIAHDSVCDALGAKKS
                     AHPFKHGVGYRLERATLISSYHCSRYNTNTGVLTEAMFDAVVKQAKLAAMPQM"
     gene            complement(1508..2083)
                     /locus_tag="VGC27_07345"
     CDS             complement(1508..2083)
                     /locus_tag="VGC27_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY0282420.1"
                     /translation="MLFYPQEKLALFIDGANLYGAAKGLQFDIDYKRLLELFARKGVL
                     VRAFYYTAVAEDQEFSPLRPLVDWLDYNGFSVVTKPLKEFTDTQGRRRVKGNMDIELA
                     IDVMEMADQVDHIVIFSGDGDFRRLVEAAQRKGRRVSVVSTIRTQPPMVSDDLRRQAD
                     NFIELEELKPMIMREGGPRANNVGPSEQAAQ"
BASE COUNT          443 a          669 c          672 g          412 t
ORIGIN      
        1 gagccgccca tccctctgga tggccgggtt gtaaagtctg tcctccggcc gcgcgtcgcg
       61 cggaccgggg ggcccggcca tggtgaaaga tgttaattcc acgcaaaccg aagcgacttt
      121 gacatggcga agaagaaaga cgacgaccgc aagatcgtcg ccgacaaccg caaggccaga
      181 ttttcctacg ccatcgagtc ctcgctggag gcgggcatcg cactgaccgg ctccgaggtc
      241 aaatcgctgc gcaccggcaa ggcgagcatc gcggaatcct atgcccaggc caaggacggc
      301 gagattttcc tgatcaacgc caatatcccc gaatatggca aggccgcccg cttcggtcac
      361 gagcccaagc gcacgcgcaa attgctggtc cacaagaggg aagcggcgaa gctctcgatc
      421 gccatccagc gcgagggcat gacgctgatc ccgctcaagc tctatttcac gcccaaaggc
      481 atcgccaagc tcgaactcgg catcgccaag ggcaagaagc tgcacgacaa gcgcgagacc
      541 gagaagaagc gcgactgggc ccgcgacaag gcccggatta tgcggaataa gggataagcg
      601 ctggtaatcc ccagcaggtg tatcacccct gtgcgggtta aggcggattt tggactaggt
      661 aaaactatac aaaaaatttc accctgccct tgagggagcg tcgatcacgc cgaagcgctt
      721 gcgcgaaggc ggatcgggga ggggttgtgc ctcaccaata cctctccctc aacccgtctt
      781 cgctgaagct tctacgggtg atgcgcttat tccagcgaag ccttggcgta gctgggctca
      841 aggagagggt gaagtccatt cctacatctg cggcattgcc gccagctttg cctgcttcac
      901 caccgcgtcg aacatcgctt cggtcagtac gccggtattg gtgttgtagc gcgagcagtg
      961 atagctggag atcagcgtcg cgcgctccag ccggtagccg acgccgtgct tgaacggatg
     1021 ggcggatttc ttcgccccca gcgcgtcgca gacgctgtcg tgcgcaatct tgcccagcgc
     1081 caggatggcg cgcagatgcg gcatggccga aatctgcccc acgaggaatt tgcggcaggt
     1141 tttgatctcg gctggcgtcg gcttgttctc cggcggcacg cagcgcacag cgttggtgat
     1201 ggcgcattcg gcgagcgtca gtccgtcgtc ggcgcgctcg tcataggtgc cgcgcgccag
     1261 cccgtgtttc aagagcatcg cgaagagaag atcgccggcc cagtcgccag tgaacggccg
     1321 gccggtgcgg tttgcgccct tgagacccgg cgccaggccc acgatcaata agcgcgcttc
     1381 gttcgcgccg aaagtcggca ccggcgcgtt gaaaaagtcc ggataggcct tcgcgttcgc
     1441 cgcgcggaat tgcgccaggc gaggacaaag caggcaatcg tgcggcggtt cgggcgcgct
     1501 caagcggcta ttgcgccgct tgttcgctgg ggccgacatt gttcgcgcgc ggaccgcctt
     1561 cgcgcatgat catcggcttc agctcttcca gctcgatgaa attatcggct tggcggcgca
     1621 gatcgtcgga caccatgggc ggctgggtgc ggatcgtgga gaccacgctg acgcgccgtc
     1681 ccttgcgctg ggcggcttcc accaagcggc gaaaatcgcc gtcgcccgaa aagatgacga
     1741 tatggtcaac ttggtcggcc atctccatca cgtcgatagc gagttcgatg tccatgttgc
     1801 ccttcacgcg gcgccggccc tgggtgtcgg tgaattcctt gagtggcttg gtgaccaccg
     1861 aaaagccgtt gtagtcgagc caatccacca gcggccgcag cggcgagaat tcctgatcct
     1921 cggccaccgc cgtatagtag aaagcgcgca ccagaacgcc cttgcgcgcg aagagttcaa
     1981 gcagccgctt gtagtcgatg tcgaattgaa gacctttggc ggccccgtac aaattcgcgc
     2041 catcgatgaa aagtgccagt ttctcttgtg gatagaaaag cataatgatc tccttcaaaa
     2101 tattgaccgt ccgggcaatg tccccccgag gttcatgcaa ctttcacctc attcggcgcg
     2161 tccgcgcaag ccgggaatga tttcatgatc gtgatc
//
LOCUS       DASWPU010000343         4921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_107014,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000343 DASWPU010000000
VERSION     DASWPU010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107014"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..3065
                     /gene="ppc"
                     /locus_tag="VGC27_07350"
     CDS             360..3065
                     /gene="ppc"
                     /locus_tag="VGC27_07350"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY0282421.1"
                     /translation="MRAAVKDSGQGKASREVIRLLGRLLGTVIKEQYGQDAQSLSRGQ
                     ADLDLVEEIRKQSIGEHRHGVADVRLDHRLSKLEPPQVALLIRAFSVFSQLANVADDH
                     LAHIQAGPGPLQQLEAHAHVNARSVRAYLSNALLAPIITAHPTEVRRKSILDRETDIG
                     ALLTLRERANQHTGESAEIEAQLKREIKTLWQTRMLRPIRIHVNDEIENAIAVFARTF
                     LSEVPAVKRRVMRLFGLNGDVLPFLKPGSWVGGDRDGNPYVSAQTLEYAIHRQAETVL
                     DHYLEQVNLLGSDLSICDEYVSTSAALKALAAGPEHTSLQQMDEPYRRALATCYSRLA
                     ATRKRLLGQGPARTARWEAAPYARPEEFAADLTVIVDSLRQNGDGDLADGRLLDLCEA
                     VGAFGFHLAVMDLRQNADVHERALGELFREAGVVSSYAALKENKRVALLLKELATPRL
                     LRSPYRRYSDETQRELDIADKAALLKQRFGACSIANYVISKTQSVSDLLETALMMKEA
                     GLLTPGKKLSASLRIVPLFETIGDLRASAEVMGAYFDLPAVRALLEGQSRVQEVMIGY
                     SDSNKDGGYLTSNWETRSAIARLTALGRARAIKMRFFHGRGGAVGRGGGSSFDAIRAL
                     PADASCAGIRITEQGEVVASKYGDPQIGRSSLETIIVAAVLSQLNHEKDAADGEAGEL
                     LARLSEAACRAYRGLVYETKGFGRYFHESTPLPEISDLKIGSRPASRTESGRIEDLRA
                     IPWVFSWSQARVMLPGWFGFGTAVREVGMKSLRPLCQTSPFFRTTVSNMEMVLAKSSL
                     SIAQRYSELVEDKALAGEVFTRIQDEWYATVEAILVVTGQSALLERQPRLANSIRLRL
                     PYIDALNHLQVDLLRRRRAGDESEETSRAIHMSINGVSAGLRNSG"
     gene            complement(3232..3663)
                     /locus_tag="VGC27_07355"
     CDS             complement(3232..3663)
                     /locus_tag="VGC27_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0282422.1"
                     /translation="MTAYSEDNSPHPLDVALGSRIRLRRRELSLSQEQLARQVGITFQ
                     QVQKYEHGTNRVSFSRLVEIAQALRCGVMDIVGDLDKSKASSFFSHHVARLNEPGAAE
                     LLEAYAAIQSPKHRRAILNLAKQLAEGKATQLYEIDDVQSR"
     gene            4279..>4921
                     /locus_tag="VGC27_07360"
     CDS             4279..>4921
                     /locus_tag="VGC27_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY0282423.1"
                     /translation="MSIQENSADMVSGARAIGRMLGDVPPSVELLLAGSERRFVAANN
                     NTSWALNSSKRLLDLSIAIPALILLTPLLALLALLIRLDSPGPALFRQKRLGYRGRPF
                     DIFKLRTMTVQEDGDTIAQAHRDDARTTWIGRWLRAFSLDELPQLLNVVKGEMSLVGP
                     RPHARAHDEFYARLIAGYSRRQEVKPGITGWAQIHGLRGATPTVGIMSRRVDFD"
BASE COUNT          907 a         1531 c         1520 g          963 t
ORIGIN      
        1 cgattgcgct tgcgtctcgt ttcctatcca cgatctgctg gcccgcccta tactgcgtgc
       61 gatgggtccc cgggtctggg aggttcaatt tcggacggaa aaccgcccgg cgcgccttgc
      121 gcgccaattg gttccatagc gcgctttcgc gcgggcactt ttcctgaaaa cgctctgggg
      181 ggaggggggt gcggaaagtc aaaaacgaat acgcggtgta gaatctctct cccggaggat
      241 ctgaccgaat ttccagccat actgcggttt tcgccattcg gctctcgcat ttttcttgcg
      301 cggctgtagg ttgatggcgg gattccgtca gatctgaccg aagagagccg ggcaagcgca
      361 tgagagcggc agtgaaggac agtgggcagg gcaaggcgag ccgcgaggtc attcgcctgc
      421 tggggcgcct gctcggaacg gtcatcaagg agcagtatgg ccaggatgcg cagagcctga
      481 gccgcggcca agccgacctc gacctggtcg aggagattcg caagcaatcg atcggcgagc
      541 atcgccacgg cgtggccgat gtccggctcg accaccggct gagcaagctc gaaccgcccc
      601 aggtggcgct cctcatccgg gccttctccg tcttttccca gctcgccaac gtcgccgacg
      661 accatctcgc ccacatccag gcgggaccgg ggccgctgca acagctcgaa gcgcatgcgc
      721 atgtcaacgc caggagcgtg cgcgcctatc tgtcgaacgc gctcttggcg cccatcatca
      781 ccgcccatcc caccgaggtc cggcgcaaga gcatcctcga tcgcgaaacc gacatcggcg
      841 ctttgctgac gctgcgcgaa cgggcgaacc agcacacggg agaaagcgcg gaaatcgagg
      901 cccagctcaa gcgcgagatc aagacgttgt ggcagacgcg catgctgcgg ccgatccgca
      961 ttcacgtcaa cgacgagatc gagaacgcca tcgccgtctt cgcgcgcacc tttctgagcg
     1021 aggttccggc cgtcaagcgc cgggtgatgc gcctgttcgg cttgaacggc gacgtgctgc
     1081 cgttcctgaa gcccggttcc tgggtgggcg gcgaccgcga cggcaatccg tatgtctccg
     1141 cccagacgct ggaatatgcg attcatcgcc aggcggagac cgttctcgac cattatctcg
     1201 aacaggtgaa tctgctgggc agcgacctgt cgatctgcga cgagtatgtc tcgaccagcg
     1261 cggcgctgaa ggcgctggcc gccggccccg agcacacttc cctgcaacag atggacgagc
     1321 cttatcgccg cgcgcttgcc acctgctatt cgcgcttggc cgccacgcgc aagaggctgc
     1381 tcggccaagg gccggcgcgg acggcgcgct gggaagccgc cccttatgcc aggcccgagg
     1441 agttcgcggc cgacctgacc gtgatcgtgg actcgctgcg ccagaacggc gacggcgatc
     1501 tggcggacgg aaggctgctc gatctgtgcg aagcggtcgg cgccttcggc tttcatctgg
     1561 cggtgatgga tctgcgccag aacgccgacg ttcacgaacg cgcgctcggc gagcttttcc
     1621 gcgaagccgg ggtggtttcg tcctatgccg ccctcaagga gaacaagcgc gttgcgctgc
     1681 tgctcaagga actggcgacg ccgcgtctgc tgcgttcgcc ttatcggcgt tattcggatg
     1741 agacccagcg cgaactcgat atcgccgaca aggcggcgct tctgaaacag cgcttcggcg
     1801 cctgctccat cgccaattac gtgatctcca agacgcaaag cgtatccgac cttctggaaa
     1861 ccgctttgat gatgaaggag gccgggctgc tgacgccggg caagaagctt agtgcttccc
     1921 tgcgcatcgt acctttattc gaaaccatcg gcgatctgcg cgccagcgcc gaggtgatgg
     1981 gcgcctattt cgacttgccc gcggtgcgcg cgctgctgga gggccagagc cgcgtccagg
     2041 aagtgatgat cggctattcc gacagcaaca aggacggcgg ctatctcacc tccaattggg
     2101 aaacccgctc cgccatcgcc cggctgacgg cgctcggccg cgcgcgcgcg atcaagatgc
     2161 gcttcttcca cggccgcggt ggcgcggtcg gccgcggcgg cggttcgagt ttcgacgcca
     2221 ttcgcgcgct tcccgccgat gcctcctgcg ccggcatccg catcaccgaa cagggcgagg
     2281 tggtggccag caagtacggc gatccgcaga ttggtcgctc cagcctggag accatcatcg
     2341 tcgcggccgt gctgtcccag ctcaatcacg agaaggatgc cgccgacggc gaagcgggag
     2401 agcttctggc gcggttgagc gaagcggcct gccgcgccta tcgcggcctg gtttacgaga
     2461 ccaagggctt cggccgctat ttccacgaat cgacgccgct gccggaaatt tcggacttga
     2521 agatcggcag ccgcccggcg tcgcgtaccg agtcggggcg gatcgaggat ttgcgcgcca
     2581 ttccctgggt gttttcctgg tcgcaggcgc gcgtcatgct gcccggctgg ttcggtttcg
     2641 gcacggcggt gcgcgaggtc gggatgaaat ccctgcgtcc gctgtgtcag acctcgccgt
     2701 tcttccgcac caccgtgtcc aacatggaaa tggttctcgc caagtcgagc ctttcgatcg
     2761 cgcagcgtta ttccgagctc gtcgaagaca aggcgttggc gggagaggtg tttacgcgca
     2821 ttcaagacga atggtacgcc accgtcgaag ccatcctcgt cgtcaccggg caatccgctt
     2881 tgctcgaacg ccagccccgg ctggcgaact cgatccgtct caggcttccc tatatcgacg
     2941 cgctgaatca cctgcaggtc gatctgctgc gccgccgccg cgccggcgac gaaagcgaag
     3001 agaccagccg cgccattcac atgtcgatca acggcgtctc ggccggcctg cgcaacagcg
     3061 gttaggattt agagcgcaat ccggcaaagt gtgaagcggt ttgccgtcag attgcgcgaa
     3121 aaacaagaga tcgagagtgt gatccgattc aattaaatcg gatcacactc taggtaccga
     3181 gccgcccccg gggaggggcg gttcggggca tgatcaagaa gcaagacttg actatcgcga
     3241 ctgcacgtcg tcgatctcgt aaagctgcgt cgcttttcct tctgcgagtt gcttggcgag
     3301 attgagaatg gcgcgcctgt gtttgggcga ttggatggcc gcataggcct ccaacagttc
     3361 cgcggcgccg ggttcgttca agcgggcgac gtggtgcgag aagaacgacg acgctttcga
     3421 cttgtcgaga tcgccaacga tgtccatcac gccgcagcgc agtgcctgtg cgatttcgac
     3481 gagccgggag aaactcaccc ggttcgtacc gtgctcgtac ttctgcacct gctggaatgt
     3541 gatgccaacc tgacgggcaa gttgttcttg cgaaagactg agctcccgcc gccgcagccg
     3601 gattctggaa cccagcgcga cgtcaagcgg gtgcggagaa ttatcctccg agtaagcggt
     3661 catacttggt ccctttggtt tcatgaatca gagcttcatt cttcaatctt gtctatcagt
     3721 aaactgccgc aggtggcagg cgcctcacct ataagttgaa gtcagaacac aaacacaccc
     3781 tctttgccag acgtcatttg caagtcaagt taatatcagt acttacgctg aagccggagg
     3841 cacacttcgt agccacgctg acggaagcat gacgatgtta tgtcaaatcg tgcgcgatac
     3901 gcatacagac gaattgtctc tgcgcggatt aatggcgtct ttcggtgtgg ttaaagaaga
     3961 ttgggagcgg ttccaggtct tgctgccgac tttattccgt ttccaagaaa cctattcgac
     4021 agcgctgtca ccatttcatt ccggcaactg aaaacgagct tggcctgcct ttcccgatgc
     4081 ggagcagttg acggctgaat atgtgacggc ggcggcaagt taaccaagcg ctgtgtcggc
     4141 ttggcacgtc tgacggcgaa gccattcttt aaatgcgcat ttccggcact cgttcgtcgc
     4201 gatgtggaac gattctctgc gaatcggttg gcttggctga tgcagcgaca ggctcggaac
     4261 tcctgcccga gactgcgagt gagcattcaa gaaaactctg ccgacatggt gtccggcgcc
     4321 cgggcaattg gccgcatgct gggcgatgtg ccgccatcgg tcgaactgct gctggcgggc
     4381 agcgagcgca ggttcgtcgc cgcgaacaac aacacgagct gggcgcttaa ttcctccaag
     4441 cgcttgctgg atctttctat cgccatcccg gcgttaatcc tgttgacgcc gttgctggcg
     4501 ctcctggcgc tcctgatccg tctcgattcc cccggcccgg cgctgtttcg ccaaaaacgc
     4561 cttggctatc gcggccgccc tttcgatatc tttaaactgc gcacaatgac ggttcaggaa
     4621 gacggcgaca cgatcgcgca ggcccaccgg gacgacgcgc gcacgacttg gatcgggcgc
     4681 tggctcaggg ctttcagcct cgacgaactg ccgcagctcc tcaatgtcgt caaaggcgag
     4741 atgtcgctgg tgggtccgcg cccgcatgca cgcgcccatg acgaattcta cgcccgtctg
     4801 attgcgggtt actcgcgccg tcaggaggtc aaacctggca tcaccggctg ggctcagatc
     4861 cacggcctgc gcggggccac gccgacggtc ggcatcatgt cccgccgcgt cgatttcgac
     4921 g
//
LOCUS       DASWPU010000344         7068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_2606, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000344 DASWPU010000000
VERSION     DASWPU010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2606"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2182)
                     /locus_tag="VGC27_07365"
     CDS             complement(<1..2182)
                     /locus_tag="VGC27_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0282424.1"
                     /translation="MGGALAQGAEVHVPAQSLAQSLKDIAHQTGANILFAPDAVRGRQ
                     ASALDGKMSAQDAVGRLIAGTNLQVVLDGSGGLIVRPASIETEAPKPVVPAAPQESFE
                     TETIIVTGIRGALQRDLDIKRFSHGLVDAITMEDMGKFPDSNLATALMRVPGVTVNRG
                     VTSMNGINSTTGDPTEITVRGFGPTFNETLFDGRKISSGVSNRAFDFSALNSDLVQEV
                     DILKSPDPSLSAGAIGATINIKYPKPLDNPGLKFTASVSTTYAPEEGKFTPNGNILFS
                     NSFAGDRVGILLAGAYAETKSRSNEVTVWGWEGTYLDPCQFADAITACGATLAPDNTR
                     PVWFIQDYGIYQIHNWQMRENGLAVVQWQPTDALLVTLNGNFSRNDLKEQQYNYAIWN
                     NPNEMRNVTTSKNGTIIDFIRTNTPTDFGSQLNEQVLQSYDAGLNVTWTVNNKLTVTA
                     DADMALSSLNPGGQFGDFSADVGYGPSNPAGTNGNNMGMVVSAGDGHALPYYSSYGPN
                     GDRSQFLNPDIMGSHVMVLMSQRNRNVVNQAKLEGSWEEHRYQVTAGLQYEANHMKLA
                     NYQNFANNQWQAYAGYGPASNNYYASGANAGLPAGVALPSSMFTKSFSTANFIPGWSG
                     SHALPPRILAFDARAVFEYLESLGNPATPTDIPGFNWGCCDPAYTGSFEVVLDPSSFQ
                     RIYEDNYAGYLAVTGDTVIGGMPLRFHAGLRSEYTDLTSTGIMRL"
     gene            complement(2328..3290)
                     /locus_tag="VGC27_07370"
     CDS             complement(2328..3290)
                     /locus_tag="VGC27_07370"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0282425.1"
                     /translation="MSAVTNVSLEIRAEAAAWLARLRADDKNSGDERAFRAWLAEDPR
                     HAAAFEAVTEVWEVAGVAWTEPAASFPRPAGLTRRKALLAGVGSLAAAGISFAFWQSA
                     YAGVYETAIGEQKHVVLPDGSQAFLDTDTRIHAAYDSQMRIVALERGRCNFHVMESDE
                     RPFVVDAAAQRIVAARTTFDVRRDGDEVCVVMLQGSASIGSRNAPARRHEVLKSGERL
                     VAADGRARIDKPNLVPLLAWQTGQAVFDNDTVAWAIREMNRYSVVKIEVTDPVVARMR
                     ISGVYRVGDNAAFALSIAALLPVVVELDTDQVRLVVDQARINRT"
     gene            complement(3292..3777)
                     /locus_tag="VGC27_07375"
     CDS             complement(3292..3777)
                     /locus_tag="VGC27_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0282426.1"
                     /translation="MTGLPTERPYWAKLLKQVARHTRGRGDAEDLLHSAYIRLEHYRT
                     EHVVDNPGAFLVRTAANIAIDIHRHEKFWEPGDTRRDGQCPDDAPLQDEVIAARARLW
                     RVKEGLAKLPTRTREIFLMHRLNGLKYREIAVHFGISQSAVEKHIAKAALFLTEWSQD
                     W"
     gene            complement(4004..5065)
                     /gene="galT"
                     /locus_tag="VGC27_07380"
     CDS             complement(4004..5065)
                     /gene="galT"
                     /locus_tag="VGC27_07380"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEY0282427.1"
                     /translation="MQTISDFKPSTFKMPDGRLLHLYGDFTGTPEWPRTKAVRETGIS
                     HRRWHPVRREWVVYSAHRQTRTYKPATDACPFCPQAPDGEIPLKDFSIAVFDNRFSSF
                     QKISPKPASPAFGLPADSAAGNCEVIVYSADHKASMSTLPQDRRELLVKVWGDRVRAL
                     MRMPAVQFVMPFENRGEEAGVTLHHPHGQIYAFSYLPPVIEVMAQSFREGYDLTKLTS
                     SATHEVSRTKSMVAVVPPYARFPYEVWMVPKEFRASPADLTAGEIGDCARLLACVAKK
                     YDTIFNRVCPYVMIVYAAPRGMEDYFPFHIQFYPLLRTPHKLKFLAGCELGAGSFLAD
                     ILPEAAARALREVRVKDAP"
     gene            complement(5390..5932)
                     /locus_tag="VGC27_07385"
     CDS             complement(5390..5932)
                     /locus_tag="VGC27_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282428.1"
                     /translation="MSGGKKCKIVRVKNRLAGILRSPGGISRTEAVTAATASIESLRE
                     EYVKLIPLEIAALETIVAEAGADEIDARDIERLLDRAGRLLTLSGTFGYSLLDAVAKC
                     FCDLAAGMIEKGITCTGPITVHLRAMRLVCPGSPALAEAESRGILDELLKIHAHFGLT
                     PHGREDAFPSIEGNDANRLE"
     gene            complement(5929..6513)
                     /locus_tag="VGC27_07390"
     CDS             complement(5929..6513)
                     /locus_tag="VGC27_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0282429.1"
                     /translation="MDEKLKFSQITALVVDNDQYATNILGQILRGFGLAKHLVAENGE
                     DGKRHLERSRFDLIICEAVLPDMPAGELVRWIRRHENPLIKYLSIVTLTGYTQPSNVF
                     AVRDSGTNTVVGKPVAPNVLFDHIAWITRADRPFIETDSYVGPCRRFKNMGPPDGIGR
                     RKTDLAAEVGAAKEPNLSQEEIDAFIRPTRVAIE"
     gene            complement(6553..6939)
                     /locus_tag="VGC27_07395"
     CDS             complement(6553..6939)
                     /locus_tag="VGC27_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282430.1"
                     /translation="MSETPGRHLGSAALRLLKGVAGELRDLAIETARFGEMLADDSSI
                     AGDNRAIGLLQRFDLFAQNLQAQALLIDKLSVHLEKDAVDVAELHQLIGNVPFFDLRE
                     RLRAALNGTSGEAAAPAEKAAEEHWF"
BASE COUNT         1349 a         2147 c         2062 g         1510 t
ORIGIN      
        1 gaagccgcat gatgcctgtg gacgtcagat cggtgtattc cgaacgcagg ccggcgtgga
       61 atctgagggg catcccgccg atcaccgtgt cccccgtgac ggccagatat cccgcataat
      121 tgtcttcgta gatgcgctgg aagctcgagg gatcgagaac cacctcgaag ctgccggtat
      181 aggcaggatc gcagcatccc cagttgaagc ccgggatatc cgtcggagtc gccgggttgc
      241 ctaagctttc caggtactcg aagacggccc gtgcatcgaa cgccaagatc cgcggcggca
      301 gagcatggga gccgctccag cccgggatga agttcgccgt gctgaagctc ttggtgaaca
      361 tgctgctcgg cagagcgaca ccggccggaa gaccggcatt cgcaccggac gcgtagtaat
      421 tgttcgaggc gggaccgtaa ccggcatagg cttgccactg gttgttggcg aaattctggt
      481 aattggccag cttcatatgg ttggcttcgt actgcaggcc cgctgtgact tggtagcggt
      541 gttcttccca gctcccttcc agtttggcct ggttcacgac gttccggttc cgctggctca
      601 tcaacaccat tacgtgcgaa cccatgatat cggggttgag gaactgcgaa cggtcgccgt
      661 taggaccata ggagctatag tagggcagtg cgtgcccatc gcccgccgaa acgaccatgc
      721 ccatattgtt gccgttggta ccggcggggt tggacgggcc atagcccaca tccgcactga
      781 aatcgccgaa ttgaccgccg ggattgagcg aggaaagcgc catatccgca tcggcggtga
      841 ccgtaagctt gttgttcacg gtccacgtca cgttcaggcc ggcatcgtag ctttgcagca
      901 cctgttcgtt gagttgggaa ccgaaatcgg tgggtgtgtt ggtgcgaatg aagtcgatga
      961 tggtcccgtt cttgctcgtc gtgacgttcc tcatttcgtt cggattattc cagatggcgt
     1021 agttgtattg ttgctccttg agatcgttgc gggaaaagtt gccgtttagc gttaccagta
     1081 aggcgtccgt cggctgccac tggacgaccg caaggccgtt ttcccgcatc tgccagttat
     1141 gaatctggta gatgccataa tcctggatga accagaccgg tctcgtgttg tcgggcgcaa
     1201 gcgtagcgcc gcaggccgtg atagcgtccg cgaactggca cgggtcgagg taggtgcctt
     1261 cccaccccca aacggtgact tcgttgctgc ggcttttggt ttccgcgtag gcgccggcca
     1321 gaagtatgcc gaccctgtct ccggcgaaac tgttgctgaa tagaatattg ccgttcggcg
     1381 tgaatttgcc ttcttcgggc gcataggtgg tggaaaccga agccgtgaat ttcaacccgg
     1441 gattatccag gggtttgggg tatttgatat tgatcgtcgc gccgatcgcc cccgccgata
     1501 gcgaaggatc ggggcttttc aggatatcga cttcctgcac caagtcggag ttcagggcgc
     1561 tgaagtcgaa ggcgcggttg ctgacgcccg acgagatttt gcggccgtcg aagagcgttt
     1621 cgttgaaggt cgggccgaag ccgcgaacgg tgatttcggt ggggtcgccc gtggtggagt
     1681 tgatgccgtt catgctggtg acgccgcgat tcaccgtcac gccggggacg cgcatcaagg
     1741 ccgtggcgag gttggaatcc gggaacttgc ccatatcctc catcgtgatg gcgtcgacga
     1801 gaccatgcga aaaccgcttg atatccaagt ctctctgcaa ggccccccgg atgcccgtca
     1861 cgatgatcgt ttccgtttcg aagctttcct gcggcgcggc gggaaccacc ggcttcggcg
     1921 cctccgtctc gatgcttgcc gggcggacga tcaagcctcc gctgccgtcc agaacgacct
     1981 gcagattcgt accggcaata aggcggccca ccgcgtcttg cgcggacatc ttgccgtcga
     2041 gggcagaagc ctgccggccg cgaaccgcat ccggagcgaa caagatgttc gcaccggttt
     2101 ggtgggcgat gtccttcagc gactgggcca gcgattgggc gggcacatgg acttcagcgc
     2161 cttgcgctaa tgcccctccc attcccaaaa ccgatgtagc tgcaatcgca aaaactagcc
     2221 agcgtgtccc ctttttgccg ctcccgtcca tgttccctca gtatgcggac acgccgccat
     2281 gcacgtgtga ggttggatga tgataagcat ttcctcgtct ctgtttttca ggttctgttt
     2341 atccgggctt gatcgaccac aagcctgacc tgatcggtat ctagctccac aacgaccgga
     2401 agcaatgcgg cgatagaaag ggcgaaagcc gcattgtcgc cgacgcgata tacaccgctt
     2461 attctcattc tcgcgacgac cggatccgtc acctcgatct tcaccacgga atagcggttc
     2521 atctcccgga tggcccaggc aaccgtatcg ttgtcgaaca cggcctgtcc ggtttgccag
     2581 gctaacagag gcacgaggtt cggcttgtcg atgcgggccc gcccatccgc ggcgaccaga
     2641 cgttcaccgg acttgagcac ctcgtgacgc cgcgccggag cgttcctgct tccgatcgag
     2701 gccgatccct gcaacatgac cacgcagact tcgtcgccgt cgcgccgcac gtcgaaggtg
     2761 gtccgcgcgg cgacgatccg ctgggccgcg gcgtccacca cgaaaggccg ctcgtcgctc
     2821 tccatcacat ggaaattgca gcggccccgt tcgagagcga cgatgcgcat ttgactatcg
     2881 taggccgcgt gaatgcgcgt atcggtgtcg aggaaagcct gagacccgtc cggcaggacg
     2941 acatgcttct gttcgccgat ggcggtctca tacacgcctg cataggcgct ttgccaaaac
     3001 gcaaaactta tccccgccgc cgccagcgac cccaccccgg cgagcagcgc tttgcggcgc
     3061 gtcaggccag caggacgcgg aaaggacgcc gcaggctccg tccaggctac gcctgccact
     3121 tcccagactt ctgttacggc ttcgaacgcc gcggcgtgac gcggatcctc ggccaaccac
     3181 gcccgaaagg cgcgctcatc gccggaattc ttgtcatctg cgcggagacg cgcaagccat
     3241 gccgccgcct ccgcccttat ctcgagagaa acgtttgtca cagccgacat gttaccaatc
     3301 ttgactccat tcggtcaaaa acagcgccgc cttcgcgata tgcttttcca cggcgctttg
     3361 gcttatcccg aaatgaaccg cgatctccct gtatttgagc ccattaagcc tatgcattaa
     3421 gaatatctcg cgcgtccgtg tcggcagctt ggccaaccct tccttcactc tccacagacg
     3481 cgcacgcgca gcaattacct catcctggag cggtgcgtca tcagggcact gtccatccct
     3541 tcttgtgtcg cccggttccc agaacttctc atggcggtga atgtcgatgg cgatgttcgc
     3601 ggccgtacga acgagaaaag cgcccggatt atcaacgaca tgctcagtgc ggtagtgctc
     3661 cagccgaatg taggcggagt gcagcaaatc ctcggcatca ccgcggccgc gcgtatggcg
     3721 tgcgacctgt ttcaaaagct tcgcccaata cggccgttct gtaggcagtc cggtcatcct
     3781 gccccccccc gggcaccttt ttgaccagta tagcaggaac aaggacatcc aagcccctaa
     3841 attttccgaa ttgggtggtg aggaaacccc cttgtcgttc gtcaagcgtc gaaatgttgc
     3901 tgctgccgct cccaatctcc aggctcaagc ccggcgggaa ttccgtcgtc agtcaaatat
     3961 gttacagcgc cttagcgcac ggacgccttg gcggtgaacg tgctcatggg gcgtccttca
     4021 ctcttacctc tcgtaaagcc cgggccgccg cttccggcag aatatccgcc aggaacgatc
     4081 cggcgccgag ttcgcagccg gccaagaact tcagtttatg cggagtgcga agtagcgggt
     4141 aaaactgaat gtggaagggg aagtaatctt ccatgcctcg cggcgcggca tagacgatca
     4201 tcacataggg gcaaacgcga ttgaagatgg tgtcgtactt cttggccacg catgccagca
     4261 gacgcgcgca atcgccgatc tcgccggccg tcaaatccgc gggcgatgcc ctgaattctt
     4321 tcggaaccat ccaaacctcg taagggaacc gcgcataagg cggcacaacg gccaccatgc
     4381 ttttggtccg ggagacttca tgcgttgccg aagatgtcag tttcgtcagg tcgtatccct
     4441 ctcggaaact ttgcgccatc acctctatga cgggtggaag gtaggaaaaa gcataaatct
     4501 gtccgtgcgg atggtgcagt gtgacgcccg cttcctcgcc gcggttctcg aacggcatga
     4561 cgaattgcac cgccggcatc cgcatcaagg cgcgcacgcg gtcgccccac accttgacga
     4621 gaagctcccg ccggtcctga ggcagggttg acatcgacgc cttgtgatcg gcggaataga
     4681 cgatgacctc gcaatttccg gcggcgctgt cggccggcaa gccgaaggcc ggggatgccg
     4741 gcttgggaga gattttctgg aacgacgaaa agcggttgtc gaacaccgca atcgaaaagt
     4801 ctttcagggg gatttcgccg tcgggcgctt gcggacagaa aggacatgcg tccgtcgccg
     4861 gcttgtaagt tcttgtctgg cggtgcgcgg aatagaccac ccattcgcgc cgcaccggat
     4921 gccagcggcg atgcgagatg ccggtttcac gtaccgcctt ggtccgcggc cattccggcg
     4981 tgccggtgaa gtcgccatag agatgcagca gccggccgtc cggcatctta aacgtggacg
     5041 gtttgaagtc ggaaatcgtc tgcatgtgag tcataagaga gcggtcccgc tggcttgatt
     5101 gtgccgatat ccctgtgaac gagacgccgg actgcttggg aagttccagg agcacactca
     5161 acccctttat atatttatat gataaatacg tattctgtaa agtgatatgg aaattggcgc
     5221 ggagcgaggc ggccttcggg cggcgcgctg taccatgcac actcggcaag atggagcggc
     5281 gcattccaaa gccttgggcc gaacctcctg gcgggataaa ttttccataa attcaatggc
     5341 ttacagaaag ccgtaccagc ccgattacgg ggcaagcatt tcaatcggac tattcaaggc
     5401 gattggcgtc gtttccttcg attgacggga aggcatcttc acgtccgtgc ggcgtcagcc
     5461 cgaaatgggc gtggattttc agaagctcgt ccaatattcc ccttgattcc gcctcggcca
     5521 atgccggaga ccccgggcag acgagccgca tggcgcgcaa atgcacagtg atcggcccgg
     5581 tgcaggtaat gcctttctcg atcatgccgg ccgccaggtc gcaaaagcat ttggcgaccg
     5641 cgtcgagcag cgaatagccg aaggttcccg acagggtcag caatctgccg gcgcggtcca
     5701 gcaatcgttc gatgtcccgc gcgtctattt cgtccgctcc ggcttcggcc acgattgttt
     5761 cgagcgcggc gatctcgagc ggaatcagct tcacatattc ctcgcgcaag gattcgatgg
     5821 acgccgttgc ggcggtaacg gcctcggtcc ggctgatgcc gccggggctg cgcagaatcc
     5881 cggccagccg gtttttgacc ctgacgatct tgcacttttt tccgccgctc attctatcgc
     5941 gacccttgtt ggtctgatga aggcatcgat ttcctcctgc gacagattgg gttcttttgc
     6001 ggcgccgact tcggccgcca gatcggtttt tctacggccg ataccgtctg gaggccccat
     6061 attcttgaag cggcggcacg gcccgacata gctgtcggtc tcgataaagg ggcgatccgc
     6121 tctcgttatc caggcgatat gatcgaacag cacgttgggc gcgaccggct ttccgacgac
     6181 cgtgtttgtg ccgctgtccc gcacggcgaa gacgttcgac ggctgagtgt aaccggtcag
     6241 ggtgacgatg gagaggtatt tgatcagcgg attctcgtga cgccttatcc agcgaaccaa
     6301 ttcccccgcc ggcatatccg gcagaaccgc ttcgcagatt atcaggtcga agcggctgcg
     6361 ttcgagatgc cttttcccgt cttcgccatt ctccgcaacc aggtgttttg ccaacccgaa
     6421 gccgcggagg atttgcccca ggatgttggt tgcatactgg tcgttgtcga caacaagcgc
     6481 cgtgatttgc gaaaacttaa gtttctcgtc cacaacacat cgcccgcgcc cgcacgcgct
     6541 ccactttttt gctcagaacc aatgttcttc ggcggctttt tccgccggcg ccgccgcttc
     6601 gccgcttgtt ccgttcaacg ccgctcgcag cctctctctc aggtcgaaga acggaacgtt
     6661 tccgataagc tggtgcaatt cggcaacgtc caccgcatcc ttctccagat gcacggacag
     6721 tttgtcgatc agcagcgcct gcgcctgcag attctgcgcg aagagatcaa atcgctgaag
     6781 cagcccgatg gcccggttgt cgccggcgat gctgctgtcg tccgccagca tttctccgaa
     6841 ccgcgccgtc tcgatcgcca agtcccgcaa ttcgccggcg acgcctttca gcaaccgtag
     6901 cgcggccgaa ccaagatgcc tacccggcgt ttcactcatc gaagaactcc acgtccggcg
     6961 tttccgagtg cttgatctcc gccttcaacg cggtgagttc gcgctcgacg agctggcgcg
     7021 ccgtgtcggg aacgacgact tgccgcgcct ttccgcttac cggcatgt
//
LOCUS       DASWPU010000345         6262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_51702,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000345 DASWPU010000000
VERSION     DASWPU010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51702"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2909
                     /locus_tag="VGC27_07400"
     CDS             <1..2909
                     /locus_tag="VGC27_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0282431.1"
                     /translation="WLQAGLLSATSILALCPAAYAQAASDEVETVVVTGIRASLRTSQ
                     EIKQSSSGVVDAITAEDIGKFPDTNLAEAIQRIPGVTIDRLNGEGARVTVRGFGPEYN
                     MVTLNGRSMPGAINANTQAATRSFDFANLASDGIAGITVTKTGRADVVSGGIGSTINI
                     KTARPFDYPGFVAAGTAKVSFDTTNRVGDDYTPNFSGLVSDTFFGDKLGILLSGSYAA
                     RNSAVESATVSGWLDVQHLNDDGTLWNDLGTGVINSNNKNPQQRIWAPRGMGYGYQED
                     QRSRTNAQAVVQYRPQENITATLDFTYAMFKNHSVQHTFGLWYTYGPNFYHGVVNEHG
                     TAIDVDTFADDMSYSTFDNHIRNELTSIGLNLEWEATDHLTVTFDAHHSLMMSGGDPR
                     GNNTFGIQGQTPSIMTAGQTKYYRTGDQEIPQGWFDFVAPWTAGTLTTDTISPLFYQA
                     NNNVFNTKIDEARLDTVWTNTNKGLTSVQFGIQVKKMQTHAAAWNSFIGTGFYDAADA
                     GMLPASVYRKIPTSGLLNSFSGGGSDLQIAPYYFEYDLADFVKYTMPKYSYGPSGIYP
                     PSSPTNDDNIQEITKAVYGQANFDLDLFDRPFKMTAGIRYEAVDVTARSLQKIATAVS
                     WDNPTEFHTIFADNAAFSKVQARNYEFLPNIDASYEVVDNLLIRSAYSKTITRSNLPS
                     MVGTTSVGITPKPLASPANAGNPGLLPYESHNWDTTVEWYYGLDSYVSANYFSKDVVN
                     FIANGITEESINGITDPYLGAQRNAAREALIAGGNPAPGDYDIFYQMVAMYGQPAFTG
                     QPGDPLKIFTVQRPTNANKLNTHGFEFAWQHTFGESGFGLQASWSIPLGGAKFDPLGP
                     DGQFVLAGLSKSYGVMAYYEKYGFQGRLAFTHRDAFLAGVGQPQNAYEPTYTAPYNQL
                     DASAAYDLTDNISVVFDGINLTGEGIKQYGRYKEQFLGAYQGGARYELGVHVKF"
     gene            3184..4551
                     /locus_tag="VGC27_07405"
     CDS             3184..4551
                     /locus_tag="VGC27_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0282432.1"
                     /translation="MNSKLSSPTGRISFGEKVGYSLGDAASNLYWKTFEFYLMIFYTD
                     FFGISAAAIGTMLLVTRLADAAADPVMGAIADRTKTRWGHFRPYLIWFALPLAAAGVL
                     TFTTPDLSGDGKVVYAYITYSLLMFLYTAVNIPYTALMGVMTSNSSERTAISSIRFIG
                     AFTAGIFVQYLTPSFVRFLGRGNDVLGWQLTMVVYGILAVVMLLVCFASTRERVVPPP
                     QQKSDLGSDLKGLFSSRAWQVLFGVMLLVLAALAIKGSDSAYYFKYFVRNESLLPSFL
                     VSNGFAFVAAVAVTSFLAKIVGKKALFMFAIGAGGLVIGGFWMVKPTDIVAIFALQII
                     SSFIIGFNSPLVFAMFADIADEAEWRTGRRNTGLVFASAIFSTKAGWAFGAWMFGLLL
                     SYYGYVPNVEQSAPALFGIVLATSWVPCILMAAAALLMQLYPLTEAQMVKIEADLRAR
                     RGEAA"
     gene            4548..5513
                     /locus_tag="VGC27_07410"
     CDS             4548..5513
                     /locus_tag="VGC27_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 17 protein"
                     /protein_id="HEY0282433.1"
                     /translation="MKRVLLCILFFVGTLASAGAADDLATQGWHGNAIAYSGYRAGQH
                     PDLGRFPSPEQVLEDLRILEKNWKYIRVYSADRHAEDVLEAIRREKIGLKVMLGVWLD
                     GRPDRLNGNSMQIVSAVRLANEYPDIVVAVNVGNEALVSWSDHRLAEPLVLSYVAQVK
                     AAVRCPVTVADDLLYWYDPAAKLVGAVDFITMHAYPVWNGEDIDTAMASTIRIYEAVR
                     AAHPDKTIVIGEAGWPTFTDSPENAPGAGSEAKQLRYYQELNAWAKAAGVTVFVFEAF
                     DEPWKGSGTEGHWGLFSEARKAKAAMRELYPERSAEPSPEQSSSP"
     gene            5526..>6262
                     /locus_tag="VGC27_07415"
     CDS             5526..>6262
                     /locus_tag="VGC27_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282434.1"
                     /translation="MALFLMSLMSSSDQWMFVSSTGLWKPRSPRHEEPYRVTRSLSRS
                     VSGNKLIFEEVNHDLGFALTSTRMCSDRVFSAAMIKRDEFLGEGPRYRRRGAGSFNKG
                     VSSMFKGWMLAVAAGTMMAASAQPAAMPWLDPAKPAEQRAAAAVAAMTLDEKLTLIFS
                     YSEPDTLDKEPDDVVPAAMKAEVREKMVPASAGYLPGIPRLKIPGQWFTDASIGIHID
                     KGRTALPSSIATAASFDPSVAEMGGKM"
BASE COUNT         1218 a         1942 c         1839 g         1263 t
ORIGIN      
        1 aatggcttca ggccggattg ctgagtgcaa cgtcgattct cgcgttgtgc ccggccgcat
       61 atgctcaggc agcatcggac gaggtcgaaa ccgtcgtcgt cacgggcatt cgcgcgtcgt
      121 tgcgcacgtc tcaggagatc aagcagtcgt cgagcggtgt cgttgacgcg atcaccgccg
      181 aagacatcgg caagtttccc gataccaatc tggccgaagc gattcagcgc attcctggcg
      241 tgaccatcga ccgtctgaac ggcgaaggcg cgcgcgtgac cgtgcgcggc ttcggtccgg
      301 aatacaacat ggtcacgttg aacggccgtt cgatgccggg cgcgatcaac gccaacacgc
      361 aggcggcgac ccgttcgttc gactttgcga atctcgcgtc agacggcatt gccggcatca
      421 ccgtcaccaa gaccggccgc gccgatgtgg tgtcgggcgg tatcggttcg acgatcaaca
      481 tcaagacggc ccgtccgttc gattatccgg gcttcgtggc tgccggtacg gccaaagttt
      541 ccttcgacac gaccaaccgg gtgggtgacg attacacgcc gaatttctcc ggcctggtca
      601 gcgatacctt cttcggcgac aagctcggca tcctgctcag tggttcctac gcggcgcgca
      661 acagcgcggt cgaatcggcc accgtcagcg gctggcttga cgtgcagcat ctgaacgacg
      721 atggcacgct ctggaacgat ctcgggaccg gcgtgatcaa cagcaataac aagaatccgc
      781 agcagcgcat ttgggcgccc cgcggcatgg gctacggcta tcaggaagac cagcgttccc
      841 gtaccaatgc gcaggccgtt gtgcagtatc gtccgcagga aaacatcacg gccacgctcg
      901 actttaccta tgcaatgttc aagaaccaca gcgttcagca tacctttggc ctgtggtaca
      961 cctacggccc caacttctat cacggcgttg tcaacgaaca cggcacggcg atcgatgtgg
     1021 atacctttgc cgacgatatg tcgtattcga cgttcgacaa tcacatccgc aacgagttga
     1081 cctcgatcgg cctgaacctg gaatgggaag cgaccgacca cctgaccgtg acgttcgacg
     1141 cgcaccactc cctgatgatg tcgggcggcg atccgcgcgg caacaacacc ttcggcattc
     1201 agggccagac cccgtcgatc atgaccgccg gccagacgaa atactatcgc accggcgacc
     1261 aggaaatccc gcagggttgg tttgatttcg ttgcgccgtg gacggccggt accctgacca
     1321 cggacacgat ctcgccgctg ttctatcagg cgaacaacaa tgtctttaac accaaaatcg
     1381 acgaagcccg tctcgacacc gtttggacca acaccaataa gggtctgacg agcgtgcagt
     1441 tcggcatcca ggtgaagaag atgcagacgc acgctgcggc gtggaacagc ttcatcggca
     1501 ccggcttcta cgatgcggcc gatgcgggta tgctcccggc cagtgtgtac cggaagattc
     1561 cgaccagcgg cctgctgaat tccttctccg gcggcggcag cgacctgcaa atcgcgccct
     1621 attacttcga atacgacctg gctgacttcg tcaagtacac gatgccgaag tacagctacg
     1681 gcccgtccgg catctatccg ccgagttcgc cgaccaacga cgacaacatc caggaaatca
     1741 ccaaggcggt ctatggtcag gccaacttcg acctcgacct gttcgatcgt ccgttcaaga
     1801 tgacggccgg catccgttac gaagccgtcg acgtgaccgc tcgcagcctc cagaaaatcg
     1861 caacggccgt gtcgtgggac aacccgaccg agttccacac gatctttgcg gataatgcgg
     1921 cgttctcgaa agtccaggct cgcaactacg agttcctgcc gaacatcgac gccagctatg
     1981 aagtggtgga caacctgttg atccgcagtg cgtacagcaa gaccatcacc cgttccaacc
     2041 tgccttcaat ggtcggcacc accagcgtcg gtatcacgcc gaagccgctg gccagcccgg
     2101 cgaatgcggg caatccgggc cttctgccgt atgaatccca caactgggat acgacggtcg
     2161 aatggtacta tgggttggac agctacgtct cggcgaacta cttcagcaag gacgtggtga
     2221 acttcattgc caatggtatt accgaggaat cgatcaacgg catcacggac ccctacctgg
     2281 gtgcacagcg taacgctgcc cgggaggcct tgattgccgg cggtaatccg gcccccggcg
     2341 actacgacat cttctatcag atggtcgcga tgtacggcca acccgcgttc accggccagc
     2401 cgggcgaccc gctgaagatt ttcacggtgc agcgtccgac caatgccaac aagctgaaca
     2461 cgcatggctt cgagttcgcg tggcagcaca ccttcggaga gagcggcttc ggtctccagg
     2521 ccagttggtc tatcccgctc ggcggcgcca agttcgatcc gctgggtccc gacggccagt
     2581 tcgtgttggc aggcctcagc aagtcgtacg gcgtcatggc ctactacgag aagtacggtt
     2641 tccagggccg tctcgcgttc acgcaccgcg acgcattcct ggccggcgtc ggtcagccgc
     2701 agaatgcgta tgagccgacc tacaccgctc cgtacaatca gctcgatgcc agcgcggctt
     2761 acgacctcac cgacaacatc tcggtcgtgt tcgatggtat caacctcacc ggcgaaggca
     2821 tcaagcagta tggccgctac aaggagcagt tccttggcgc ataccaaggc ggcgcgcgct
     2881 acgagttggg cgtgcatgtg aagttctgat cggcccagac agtaagggga gggccgttcg
     2941 ctggagcggc ctttctctta ttgatccaag ccgccgcgca cgcacgctat ggcctgagcc
     3001 cacttcagaa cgggcatccc tcccggaact ctggctccgc cccgcgtccg attggggcgg
     3061 agcgcccttt catgcaagga tagcttttgt gtcgaaaaac tctacggcga tggaacagcg
     3121 gattctacag gcgacagcag ctaacctcga cgcccgcgcc agtgcacaca gtgggagaaa
     3181 cccgtgaaca gcaagctgtc gtcgccgacc ggccgaatct cgttcgggga aaaggtcggt
     3241 tattcgctgg gggatgcggc ctccaatctg tattggaaga cgttcgaatt ctatttgatg
     3301 atcttttata ccgatttctt cggcatttcc gccgccgcca tcggtacgat gctgctcgtc
     3361 acgcgccttg cagacgcggc ggccgatccg gtcatggggg ccatcgccga ccgcaccaag
     3421 acgcgttggg ggcatttccg tccgtatctc atctggtttg ccttgcctct ggccgccgcc
     3481 ggcgttctca ccttcacaac gccggacttg agcggcgacg gaaaggttgt ttacgcttac
     3541 atcacatatt cgctgctcat gttcctctat acggcagtga acattcccta caccgcctta
     3601 atgggcgtga tgacgtcgaa ctcgtcggag cgcaccgcga tatcctcgat ccgtttcatc
     3661 ggtgctttca cggccggtat tttcgtgcag tatttgacgc cgagctttgt gcgcttcctg
     3721 ggacgcggca acgacgttct gggctggcag ctcacgatgg tggtctacgg catcctcgcc
     3781 gtcgtcatgc tgctcgtttg ttttgcctcc acccgcgagc gggtcgtccc gccgccgcag
     3841 cagaagtccg acctcggcag cgatctcaaa gggctgttct cttcccgtgc ctggcaggtt
     3901 ctctttgggg tgatgctgct ggttttggcc gcgctcgcca tcaaggggtc ggacagcgcc
     3961 tattacttca aatatttcgt acgcaacgaa agccttctgc cgtctttcct ggtgtcgaac
     4021 ggctttgctt ttgtggcggc cgtggcggtg acatcgttcc tggccaaaat cgtgggcaag
     4081 aaggccctgt tcatgttcgc catcggcgcc ggcggcctcg tgatcggcgg cttctggatg
     4141 gtcaagccca ccgacatcgt cgcgatcttc gcactgcaga ttatctccag cttcatcatc
     4201 ggctttaact cgccgttggt cttcgccatg tttgccgaca tcgcggacga agcggagtgg
     4261 cgcaccggcc ggcgcaatac gggactggtc tttgcttcgg cgattttctc caccaaagcc
     4321 ggatgggcct ttggcgcttg gatgtttggc cttctactct cctactacgg ctacgtgccc
     4381 aatgtggagc agagcgcgcc tgcgctgttt ggcatcgtcc tggcgacctc ctgggtgcct
     4441 tgcatcttga tggccgccgc cgcgctgctg atgcagctct atcccttgac cgaagcccag
     4501 atggtcaaga tcgaagccga ccttagggcg cggcggggag aggcggcatg aaacgcgtgc
     4561 ttctttgcat cttgttcttc gtcggaacgc ttgcttctgc cggcgcggcc gacgacctcg
     4621 ctacacaagg ctggcatggc aacgccatcg cctattccgg ctaccgggcg ggacagcatc
     4681 ccgatctcgg ccgcttcccc agtcccgaac aggttctcga ggacctgcgt atcctcgaaa
     4741 agaactggaa gtacatccgc gtctacagtg ccgaccgcca tgccgaggac gtgctggagg
     4801 cgatcagacg ggaaaagatc ggcctcaagg tgatgctcgg ggtttggctc gacggcaggc
     4861 cggacaggct gaacggcaat tccatgcaga tcgtaagcgc ggtccgcctg gccaacgaat
     4921 atcccgacat tgtggtggcc gtgaatgtcg gcaacgaggc tttggtctcc tggtcggatc
     4981 atcgcctcgc cgagccgctg gtgctttcct atgtggcgca ggtgaaggcc gcggtgcgct
     5041 gtccggtaac cgtcgcggac gacttgctct attggtacga tccggcggca aagctggttg
     5101 gggccgtcga tttcatcacc atgcacgcct atccggtgtg gaacggtgag gacatcgata
     5161 cagcgatggc atcgacgatc cgcatctatg aggccgtgcg tgctgcccac ccggacaaga
     5221 ccatcgtcat cggcgaagcg ggctggccga ccttcaccga cagtcccgag aacgcgccgg
     5281 gcgccggcag cgaggccaag cagctccgct attaccagga actgaatgcc tgggccaagg
     5341 ccgctggcgt cacggtattc gtcttcgaag cattcgacga accctggaaa ggctcgggca
     5401 ctgaggggca ctgggggttg ttcagcgaag cccgcaaagc caaggcggcc atgcgggagc
     5461 tctatcccga acggtccgcc gaaccgtcac cggaacaatc atcctcccct tagaacaagg
     5521 acgccatggc gcttttcctg atgagcctaa tgagcagctc cgaccaatgg atgttcgtct
     5581 ccagcaccgg tttgtggaaa ccgcgttcgc cgcgccacga agagccttat cgcgtcactc
     5641 gaagcctctc caggagtgtc agcggcaaca agttgatttt cgaagaggtc aatcacgatc
     5701 tcggctttgc actcacctcg accaggatgt gctcggaccg tgtcttctcc gccgcgatga
     5761 tcaagcgcga cgaatttcta ggcgaagggc cgcgttatcg cagacggggc gccgggtcgt
     5821 tcaacaaagg agtaagttcg atgtttaagg ggtggatgct ggccgttgcg gctggcacga
     5881 tgatggctgc tagcgcgcag ccagcagcga tgccgtggct tgatcctgcc aaaccggcag
     5941 agcagcgggc tgccgccgcc gtcgcggcga tgaccctcga tgaaaagctc acgctgattt
     6001 tcagctacag cgaaccggat accctcgaca aagagccgga tgatgtcgtc cctgccgcca
     6061 tgaaggcgga agtgcgggag aaaatggtgc ccgcttcggc cggttatctt cccggcattc
     6121 cgcgcctcaa gattccgggc caatggttca cggatgcctc gataggaatc catatcgaca
     6181 agggccgcac ggcgttgccg tcttcgatag ccacggccgc gagcttcgat ccgtcggttg
     6241 cggaaatggg cggcaagatg at
//
LOCUS       DASWPU010000346         4255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_94720,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000346 DASWPU010000000
VERSION     DASWPU010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94720"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VGC27_07420"
     CDS             <1..188
                     /locus_tag="VGC27_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282435.1"
                     /translation="IGFVLLVFVRAVTIGKQDFTAYTSTGWQDILGLPTLTLLSYFII
                     FAVLIGVVHVIRTAVFR"
     gene            complement(256..681)
                     /locus_tag="VGC27_07425"
     CDS             complement(256..681)
                     /locus_tag="VGC27_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEY0282436.1"
                     /translation="MSTSEYLSPLVGRLLFVWFFSCQLLHYGGEWSATVSLMNFAGVP
                     AAPFLLLVVLMLLVLGSLSLLLGLHTRHGAMLLFGVTIIATVVMHSFWQIDNNELARQ
                     ADFELFSRNVALAGGLLLLVGMGPGPFALDNRQNEKRKK"
     gene            complement(800..1228)
                     /locus_tag="VGC27_07430"
     CDS             complement(800..1228)
                     /locus_tag="VGC27_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HEY0282437.1"
                     /translation="MKRHDTLIRLRRFRVDDFKRRMATLDGMKADVEKKLADLEESVA
                     RERQRAGESDIGRLAFPSFLRSIEDRRENLRATLKDIERERVQCQADLTQAFQDLKSL
                     EFANEQQEKRLAEIEARRAQSRLDEMALVRHLRKHALRGA"
     gene            complement(1225..2565)
                     /gene="fliI"
                     /locus_tag="VGC27_07435"
     CDS             complement(1225..2565)
                     /gene="fliI"
                     /locus_tag="VGC27_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEY0282438.1"
                     /translation="MQTLQKEIEAIPALRRFGRVARIEGLLVEVTGAAGAISLGGQVR
                     LTPHSGKQIPCEVVGFREGRALAMPLGVLDGMTLGARADFEGHAAAIFPCQGWLGRVL
                     DAFAEPVDGKGPLPRGQQAYPLKAQPPPPQARARVGGKLDLGVRALNAFITCCKGQRM
                     GIFSGSGVGKSTVLAMLARYTDADAIVIGLVGERGREVKEFVEDDLGEMGLKRSVVVA
                     ATSDEPPLVRRQAAYTAMTIAEQLRDQGLHVLLLMDSVTRFAMAQREVGLSAGEPPAS
                     KGYTPTVFAELPRLLERAGPAEVGCNGSITGLFTVLVEGDDHNEPISDAVRGILDGHI
                     VLDRAIAERGRFPAVNILKSVSRAMPGCNSQAEQDIVAQARKHIAVYEDMAELIRLGA
                     YKTGTNAEVDAAIGMHPQFENFLAQRKDERATLTEGYGELGRIVKSGVGKGKAS"
     gene            2823..3515
                     /locus_tag="VGC27_07440"
     CDS             2823..3515
                     /locus_tag="VGC27_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0282439.1"
                     /translation="MRVLLIEDDSAMARSIELMLRSEGFNVYTTDLGEEGIDLGKLYD
                     YDIIVLDLQLPDMSGFEVLKSLRVAKVQTPVLILSGNAIVEAKVKALGFGADDYMTKP
                     FHKDELVARIQAVVRRSKGHSQSVITTGKLTVNLDAKTVEVDGSRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLAAATNGDNYIETVWGRG
                     YVLRDPAEEAAA"
     gene            complement(3570..>4255)
                     /locus_tag="VGC27_07445"
     CDS             complement(3570..>4255)
                     /locus_tag="VGC27_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282440.1"
                     /translation="LDGNSTVDIFSVEIENGAVNLTEVGSIGGRILVSPNDLVAVDED
                     RFYVVNDRMSKTALGRWLESNLLLPRANILYFDGIKFIAAAEGLNSPLGVAMSADGRY
                     LYVPEALPRKLLTFERNPFSNQLKQVNALSIPSGLDNADVAADGSIWVAGHPKYNAML
                     RYIGDPSKPSPSEIFRVAVHDGMPQAASLVYANMGEEIGAASVGATADGHLLIGSPFD
                     DKLLDCTPP"
BASE COUNT          820 a         1328 c         1274 g          833 t
ORIGIN      
        1 tgatcggctt cgtgctgttg gtctttgtcc gcgccgtgac gatcggcaaa caggatttta
       61 cggcttacac gagcaccggc tggcaagaca ttctcggctt gccgacgctg acgctgttgt
      121 cctacttcat catcttcgcg gtgctgatcg gcgtcgttca cgtcatccgc acggcggttt
      181 tccgctaagc ctttgttgcg gcggtaaacc ggccttggcc ggcggagagc atgctttccg
      241 ccggtaaggc ggttgttact ttttcctctt ctcgttctgg cggttatcga gggcaaacgg
      301 ccccggtccc atgccgacca gcagcagcag gccgccggcg agggcgacgt tgcgcgagaa
      361 cagctcgaaa tcggcctgac gggccaattc attgttgtcg atctgccaga aggagtgcat
      421 caccacggtg gcgatgatcg tcacgccgaa cagcagcatg gcgccgtggc gggtgtgaag
      481 ccccagcagc agcgacaggc tgcccaggac aagcagcatt aacaccacca aaagcaggaa
      541 aggcgcggcc ggaacgccgg cgaaattcat cagcgagacc gttgcgctcc attcgccgcc
      601 atagtgcagc aattggcacg agaagaacca gacgaacagc agccgcccga ccagcggcga
      661 aagatactcg gaagtggaca tgaaagcctc ccctgcgtcg caggtggagg attctaaggt
      721 tgttttggcg aatagcgaat agagaatagc gaacggaaga aactcctact cgctattcgc
      781 cattctctac gcgcttttcc taggcccctc tgagcgcgtg cttgcgcagg tggcgcacca
      841 gcgccatttc gtcgaggcgc gactgggctc tgcgggcctc gatctcggcc aggcgctttt
      901 cctgctgttc gttggcgaat tccaggcttt tcaaatcctg aaaggcctgg gtgagatccg
      961 cctggcactg gacgcgttcg cgttcgatgt ccttgagagt cgcgcgcaaa ttctcgcgcc
     1021 ggtcctcgat ggagcgcagg aacgaaggga aggccagccg gccgatgtcg ctttcgccgg
     1081 cgcgctggcg ttcgcgcgcc acgctttctt ccaggtcggc gagcttcttc tcgacgtcgg
     1141 ccttcatgcc gtcgagcgtc gccatgcggc gcttgaaatc gtcgacgcgg aatctgcgaa
     1201 gccggatcag tgtatcgtgt cttttcatga cgccttaccc ttccccacgc cgcttttgac
     1261 tattctgccc agttctccat acccttcggt taacgtcgcg cgttcatcct ttctttgtgc
     1321 cagaaaattc tcgaactgag gatgcatgcc gatcgcggcg tcaacctcgg cgtttgtacc
     1381 cgtcttgtag gcgcccagcc tgatcaattc cgccatatcc tcataaacgg cgatatgctt
     1441 gcgcgcttgc gccacaatgt cctgctccgc ctgggagttg cagcccggca tggcgcggct
     1501 caccgattta agaatattga cggccggaaa ccttccgcgt tccgcaatgg cacggtcgag
     1561 cacgatatgc ccgtccaaga tgccgcggac ggcgtcggaa atcggctcat tatggtcgtc
     1621 gccttcgacc agaaccgtaa acaagccggt gatcgaaccg ttgcagccga cctctgccgg
     1681 gccggcgcgt tccagcaatc gcggcaattc ggcgaacacg gtcggcgtat agcctttcga
     1741 cgccggcggc tctccggcgg agaggccgac ctcgcgttgt gccattgcga aacgggtcac
     1801 gctgtccatc agcagcagca catgcaagcc ttggtcccgc aattgttcgg cgatggtcat
     1861 cgccgtatag gccgcctgcc ggcggaccag aggcggctcg tccgaggtgg cggcaaccac
     1921 gacggagcgc ttcaagccca tttcgcccag atcgtcctcg acgaattcct ttacttcgcg
     1981 gccgcgttca ccgaccaggc cgatgacgat ggcgtccgca tcggtgtagc gcgccagcat
     2041 ggccagcacc gtcgatttgc cgacgccgct gcccgaaaaa atgcccatgc gctggccctt
     2101 gcagcaggtg atgaaggcgt tgagcgcgcg cacgcccaga tcgagcttgc cgcccacccg
     2161 cgcgcgggcc tggggcggcg gcggctgcgc cttgagcgga taggcctgtt ggcctcgcgg
     2221 cagcggtcct tttccgtcca ccggctcggc gaaggcatcc agcacgcggc cgagccagcc
     2281 ctggcaggga aaaattgccg ccgcatggcc ctcgaaatcg gcccgtgctc ccagcgtcat
     2341 gccgtcgagt acgccaagcg gcatcgccag ggcgcggcct tcgcgaaaac cgaccacctc
     2401 gcaaggaatt tgcttgccgg aatggggggt taggcgcacc tgcccgccca ggctgatagc
     2461 ccctgccgcc cccgtcacct cgaccaaaag cccctcgatc cgggccaccc ggccgaaccg
     2521 gcgcaaggcc gggatcgctt cgatttcctt ctgcaacgtc tgcattccgc gcctttccgg
     2581 ctcgcccgtc ttaaaaaaga catcttgcag gaaaggattt aagtggcggt aaacttaaca
     2641 aatcgaatca atgagatgaa accgaggctt gggttcgttc tgttgccgtt aaggcatgtt
     2701 cgcatcccat gaaattcttc cgaacagtga aagtggatgt taaccatttc cgcttaccgt
     2761 ccagtaagcg gttaactaaa accggtggaa ttccatcggc atcatgacca aaggggtctg
     2821 acatgcgcgt gctcttgatc gaagacgata gcgccatggc gcggagcatc gaactgatgc
     2881 ttcggtcgga gggctttaac gtctacacaa cggatctggg cgaggaaggg atcgacctcg
     2941 gcaagctgta cgattacgac atcatcgtgc tcgacctgca gcttcccgac atgagcggct
     3001 tcgaagtcct gaagagcctg cgcgtcgcca aggtacagac gccggtcctc attctctcgg
     3061 gcaacgccat cgtcgaggca aaggttaagg cgcttggctt cggtgccgac gactatatga
     3121 ccaagccgtt ccacaaggac gagctggtcg cccgcatcca ggcggtcgtg cgccgctcca
     3181 agggccattc gcagtcggtg atcaccaccg gcaagctgac cgtcaatctc gacgccaaga
     3241 cggtggaagt ggacggctcg cgcgtgcatc tgaccggcaa ggaatatcag atgctggaac
     3301 tgctttcgct caggaagggc accacgctca cgaaagaaat gttcctcaac catctttacg
     3361 gcggcatgga cgagccggag ctgaagatca tcgacgtctt catctgcaaa ctgcgcaaga
     3421 agctcgccgc cgccaccaac ggcgacaatt acatcgagac cgtctggggc cgcggctatg
     3481 tgctgcgcga tccggcggag gaagccgccg cctgacgatt tgaccggggc agtgatcaag
     3541 ggcgccggca acggcgccct ttttgttttc tacggcggcg tgcagtccag caacttgtcg
     3601 tcgaacggcg agccgatcag cagatgaccg tcggcggttg cgcctacgct ggcggcgccg
     3661 atctcttcgc ccatattcgc ataaaccagc gaagccgctt gcggcatgcc gtcatgcacc
     3721 gcgacgcgga aaatctccga cggcgacggc ttcgacggat cgccgatgta gcggagcatg
     3781 gcgttgtact tgggatggcc cgccacccag atgctgccgt cggcggcgac gtcggcattg
     3841 tcgaggccgg acgggatgga cagcgcgttc acttgtttga gctggttcga gaacggattg
     3901 cgctcgaagg tcagaagctt gcggggcaag gcttccggca catagagata acgcccgtcg
     3961 gcggacatcg ccacgccaag cggggaattc agcccttcgg ccgccgcgat gaactttatc
     4021 ccgtcgaaat agaggatgtt ggcgcgcggc agcagcagat tgctttcgag ccagcgcccg
     4081 agcgccgttt tgctcatgcg gtcgttgacg acgtagaaac ggtcctcgtc aaccgccaca
     4141 agatcgttgg gactgaccag gatgcgcccg ccgatgctgc cgacttccgt cagattgacc
     4201 gcgccgttct cgatctccac cgagaaaata tcgaccgtgc tgtttccgtc caggc
//
LOCUS       DASWPU010000347         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_70615,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000347 DASWPU010000000
VERSION     DASWPU010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70615"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VGC27_07450"
     CDS             complement(<1..912)
                     /locus_tag="VGC27_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282441.1"
                     /translation="MSDSQAGVACPAGLARPSALPLSKLAVELAAIALLCPVLLYAAL
                     WNGFPFVFYDTGAYVLEGLGHSFVPERSSVYSLLLLYAGGRASLWNVALAQCLMTAFA
                     IVELARAVRPQTGLLEMLAIGAALALFTSIAWCAGQIEPDCATPLLALTFYLLAFRLR
                     QVGWVRGVLLLCVAAFATGAHPSNLGLAAGLAACIGAAKLAGLIWRDLPRPALLPPVL
                     AFALGLGLVFAANYSLTHKLFVSRSGTVFLAARLMGDGIVKRTLDDICPAQPLKLCAY
                     KDSLPPTADDWLWGDESPFNTLGRFNGT"
     gene            1529..1945
                     /gene="sdhC"
                     /locus_tag="VGC27_07455"
     CDS             1529..1945
                     /gene="sdhC"
                     /locus_tag="VGC27_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY0282442.1"
                     /translation="MAEHPQKVAARPLSPFLTVYRWPVTMATSIIHRMTGVGLAGGTL
                     LVAWWLLAVAYGPETFNFFNSTMTTPLGQIVLFGFAWSLAYHFVNGIRHLAWDFGYGF
                     ALPTANLTGILVFALSLLLAAGTFALAYLGKGGYYQ"
     gene            1942..2313
                     /gene="sdhD"
                     /locus_tag="VGC27_07460"
     CDS             1942..2313
                     /gene="sdhD"
                     /locus_tag="VGC27_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY0282443.1"
                     /translation="MSLAPLHKFKDLGSAHSGVGHFKHQRVTAIMLIPLTVWFAVSAL
                     GLVGAQEVSVLIFLSNPLNAVLLGAFILISLYHMALGLQEVILDYVPHEGAKLLLMFC
                     NYGFAFLVAAVCAVALLRITV"
     gene            2328..>2803
                     /locus_tag="VGC27_07465"
     CDS             2328..>2803
                     /locus_tag="VGC27_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0282444.1"
                     /translation="MTAYPIIEHSFDVVVVGAGGAGLRATLECASKGLKTACVTKVFP
                     TRSHTVAAQGGVAAALGNMGEDDWRWHMYDTVKGSDWLGDQDAIEYMCSVGPQAVYEL
                     EHMGLPFSRTESGRIYQRPFGGQSKNFGKGGQAARTCAAADRTGHALLHTLYQANLR"
BASE COUNT          529 a          911 c          899 g          464 t
ORIGIN      
        1 ggtgccattg aagcggccga gcgtgttgaa cgggctttcg tcaccccaca gccagtcgtc
       61 ggcggtgggc ggcaggctat ccttgtaggc gcagagcttg agaggctgtg cggggcagat
      121 gtcgtcgagc gtgcgcttga caatgccgtc acccatcagc cgcgcggcga ggaagaccgt
      181 gcccgagcgg ctgacgaaca gcttatgcgt cagggaataa ttcgccgcga acaccaagcc
      241 cagtccaagc gcgaaagcga gaaccggcgg caacagagcg ggccggggca gatcacgcca
      301 aatcaggccg gcgagctttg ccgcgccgat gcaggccgcc aaccccgccg ccaagcccaa
      361 attcgaggga tgcgcgcccg tggcgaacgc agccacgcac aagagcagca ctccgcgcac
      421 ccagccgacc tgccggaggc gaaaggcgag cagatagaag gtcagcgcca ggagcggcgt
      481 cgcgcaatcc ggttcgatct ggccggcgca ccaggcgatg ctggtgaaca gggccagggc
      541 ggcgccgatg gcgagcatct ccagcaggcc ggtttgcggc cgtaccgcgc gggcgagttc
      601 gacgatggcg aatgcggtca tcagacattg cgccagcgcc acgttccaca ggctggcgcg
      661 gccgcccgcg tagagcagca acagcgaata gaccgacgag cgttccggca cgaaggaatg
      721 gccgaggcct tcgagcacat aggcgccggt atcgtagaag acgaagggaa agccgttcca
      781 cagcgcggcg tagagcaaga ccgggcaaag cagcgcgatg gcggcgagtt cgacggcgag
      841 cttggaaagc ggcaacgcgg agggccgcgc cagcccggcc gggcatgcca ctccggcttg
      901 cgaatcgctc atcccaaatc gccgacgaaa gaattcccgc ccgaagcgag agaatgctgc
      961 atcgcccggc aaaccgcaaa ccgcgatttc acgcggaggc gcggagaacg cggagcgcaa
     1021 aagagattgt ccgagacctc cgcttctccg cgtgcgaacc caaacggcca ccataagagc
     1081 agcgatgacg agagaagggc gaatctgccg cagccagcag acctcccgtg ttgacttggg
     1141 gacctcaaaa ccaaagggcg cggaaacata tttctcacga gccgcgtttc gagcgaaaaa
     1201 cggactatgg caggtggagc ggcgccagcc gcgaccgcgc gaagagcgta tcgggcaata
     1261 cggtcgagcg cttcgacgcc ccagagggcg cccgggcgcg aagcgccctt atcatttaat
     1321 ttggcgcgca aggcgcgccg ggcgccgaaa cccttcggga cgcgccgctt ttgcccgccc
     1381 ggttcgtcgc cggcctcgtg cgtactcccc ggtacgccct tcggccggct cctgccggat
     1441 cgaggcaaaa tcggcagcgt cgcggccgtg acaaatatgt tttcgcaccc taatctccgg
     1501 ctcgttttta gccccctcga ggatcttcat ggcggaacat ccgcagaaag tggcagcgcg
     1561 cccgctttcg cccttcctga ccgtctatcg ctggcccgtc accatggcga cgtcgatcat
     1621 ccaccggatg accggcgtgg ggcttgccgg cggcacgctg ctcgtcgcct ggtggctgct
     1681 cgccgtggca tacggcccgg agaccttcaa ctttttcaac agcaccatga ctacgccgct
     1741 cggccagatc gtgctgttcg gcttcgcctg gtcgctcgcc tatcacttcg tgaacggcat
     1801 ccgccatctg gcctgggact tcggctacgg cttcgctctg ccgaccgcca atctgacggg
     1861 catcctggtc ttcgcgctgt cgctgctgct ggcggcggga accttcgccc tcgcctatct
     1921 gggcaaggga ggatactacc aatgagcctt gcgcccctcc acaagttcaa agacctgggc
     1981 tcggcgcatt ccggggtcgg ccatttcaag catcagcgcg tgacggcgat catgctgatc
     2041 ccgctcacgg tgtggttcgc ggtttccgcg ctcggcctgg tcggggccca ggaagtgtcg
     2101 gtgctgatct tcctgtccaa cccgctgaac gcggtgctgc tgggcgcctt catcctgatc
     2161 tcgctgtacc atatggcgct gggcttgcag gaagtcatcc tcgactatgt gccccacgaa
     2221 ggcgccaagc tgctgctgat gttctgcaac tacggcttcg cgtttctggt ggcggcggtg
     2281 tgcgccgtcg ccctgctcag aattaccgtc tgattccgga acctgatatg accgcttatc
     2341 ccattatcga gcattcgttc gatgtcgtcg tcgtcggcgc cggcggcgcg ggtcttcgcg
     2401 ccacgctgga atgcgccagc aagggcctca agacggcatg cgtcaccaag gtcttcccca
     2461 cccgcagcca taccgtagcc gctcaaggcg gcgttgccgc ggcactcggc aacatgggcg
     2521 aggacgattg gcgctggcac atgtacgaca cggtcaaagg ttccgactgg ctcggcgacc
     2581 aggacgcgat cgagtacatg tgcagcgtcg gtccgcaagc cgtgtacgaa ctcgaacaca
     2641 tggggctgcc gttctcgagg accgagtcgg gccgcatata ccagcgtccg ttcggtggtc
     2701 aatcgaagaa tttcggcaaa ggcggacaag ccgcgcggac ctgtgccgcg gcggatcgaa
     2761 ccggacacgc gttgttgcac actctctatc aggcgaatct gcg
//
LOCUS       DASWPU010000348         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_8815, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000348 DASWPU010000000
VERSION     DASWPU010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8815"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VGC27_07470"
     CDS             complement(<1..542)
                     /locus_tag="VGC27_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEY0282445.1"
                     /translation="MDFSDPELERYARHIVLREVGGAGQARFKAARVLVVGAGGLGSP
                     VILYLAAAGVGTIGIADFDAVSLSNLQRQIAHRTADIGRTKTASAAEAALAINPHVKI
                     ESHQLRLTQDNAPDLIGRYDIVADGSDNFATRFLVADACFFAKKTLVSAAVSEFSGQL
                     ATFKPHEKGKPCYRCLFPEPP"
     gene            801..2420
                     /locus_tag="VGC27_07475"
     CDS             801..2420
                     /locus_tag="VGC27_07475"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEY0282446.1"
                     /translation="MSQLGGQAGAFGLEAQGFRNLKAVHWNLAPAILYEHAITRGEGR
                     LAKDGPLVVLTGQHTGRSPGDKFIVKDAETEGRVWWDNNKAMSPEFAEVLLADMLAYA
                     EGRELYVKDLFASADPRYCLPVRIVSEFAWHALFSHQLLIRPKPDQLEHFTPEFTIVD
                     LPGFEAVPERHGCRSKTVIALDFTGKRVLIGGTSYAGEIKKSIFTILNYLLPVKKVIP
                     MHCSANVGADGKSAVFFGLSGTGKTTLSADASRTLIGDDEHGWGSGGVFNFEGGCYAK
                     VIRLSREAEPEIFGTTERFGTVLENVVCDPNTGALDLNDERYTENTRAAYPINFIPHA
                     SDTGRAPHPTNVIMLTADAFGVMPPIAKLSPSQAMYHFLSGFTSRVAGTEKGLGAEPE
                     PTFSTCFGAPFMPRHPSVYGNLLRDLIDRYDCDCWLVNTGWTGGPAVRGGKRMPIKVT
                     RALLAAALDGSLAEAEWRVDPFFGFRAPVAVKGVDPAILDPRNTWSNKAAYDTTAKRL
                     AGMFRDNFKTYVPYVDERVLKAADVIVKAAG"
     gene            2576..>3265
                     /locus_tag="VGC27_07480"
     CDS             2576..>3265
                     /locus_tag="VGC27_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282447.1"
                     /translation="MRLNGIAAAAAALLVLGQGALAFGTIHGLGQNAEHERITRHALA
                     CGASGLSPAQCFQPFSIDQLAGKSGTFGAVGAPDNPIRGLMDKIYAHCDNGDFMDKPG
                     YPQSKTAARQNLASCRSWMITNMNAAVADSAAMLKNGKIDDSQIPTYISCTFNGTKGR
                     AKCNALEDFGLTLHASQDFYAHSNWTDHAAPQPISITNVPGLANSVPAPWIDPSLSVP
                     FPEGLMTGCFIQ"
BASE COUNT          619 a         1052 c          988 g          606 t
ORIGIN      
        1 ggcggctcgg gaaacaagca gcggtagcag ggcttgccct tttcgtgcgg cttgaaggtc
       61 gcaagctggc ccgagaattc gctgaccgcg gcgctgacca gagtcttctt ggcgaagaag
      121 caggcgtcgg caaccaggaa acgggtggcg aaattgtccg atccgtcggc cacgatgtcg
      181 tagcggccga taaggtccgg cgcgttgtct tgcgtcagcc gcaattgatg gctctcgatc
      241 ttcacatgcg gattgatggc gagcgcggcc tcggccgccg acgcggtctt ggtgcggccg
      301 atatcggcgg tccggtgggc aatctgccgt tgcaggtttg acaggctcac ggcgtcgaaa
      361 tcggcgatgc cgatggtgcc gacgcccgcc gccgccagat agaggatcac cgggctgccc
      421 agcccgcccg cgccaaccac cagcacccgc gccgccttga agcgcgcctg accggcgccg
      481 cccacctcgc gcagcacgat gtggcgggcg taacgctcaa gctccggatc gctgaaatcc
      541 acaggactct cccatcgcgg cgggcgcgat ctggcggttc cgtcaacgcc ttataccggg
      601 aatttgcgac ctatggccgc tatcgggccg ctattgcgtc acatccgcca gctttgtcgt
      661 tgctaccttg aaattgttat ggatgataca ttgaaataaa aagatattct gcgggccata
      721 aagcagcctt ttatgccgca gcgaaagcgt cgcactttaa tccgcgcggc ctgtacatac
      781 gatgccgaca gaggagaatc atgtcacagc tcggcgggca agccggggct ttcggtcttg
      841 aagcccaagg ctttcgcaat ctcaaggcgg tgcattggaa tctggcgccg gccatcctgt
      901 atgagcacgc gatcacgcgc ggcgaagggc gcctggccaa ggacggcccg ctggtcgtcc
      961 tcaccggcca gcacaccggc cgctcgcccg gcgacaaatt catcgtcaag gacgccgaga
     1021 ccgaaggccg ggtgtggtgg gacaacaaca aggcgatgtc gccggagttc gccgaagttc
     1081 tgctcgccga catgctcgcc tatgccgaag ggcgcgaact ctacgtcaag gatttgttcg
     1141 ccagcgccga tccgcgctat tgcctgcccg tccgcatcgt ctcggaattc gcttggcacg
     1201 cgctgttctc ccatcaattg ctgatccgcc ccaagccgga tcaactggaa catttcacgc
     1261 ccgaattcac catcgtcgat ctacccggct tcgaggccgt tccggagcgc cacggctgcc
     1321 gcagcaagac ggtgatcgcg ctggatttca ccggcaagcg ggtgctgatc ggcggcacct
     1381 cttatgcggg cgagatcaag aaatcgatct tcaccatcct gaactatctg ttgccggtga
     1441 agaaggtgat tccgatgcat tgttccgcca atgtcggcgc ggacggcaag tcggcggttt
     1501 tcttcggtct gtccggcacc ggcaagacca cgctgtcggc cgacgccagc cgtaccctga
     1561 tcggcgacga cgaacatggc tggggctcgg gcggcgtctt caatttcgaa ggcggctgct
     1621 atgccaaggt gatccgcctg tcgcgcgagg ccgaacccga aatcttcggc accaccgagc
     1681 gcttcggcac ggttctggag aacgtggtct gcgatcccaa caccggcgcg ctcgacctca
     1741 acgacgagcg ctacaccgag aacacccgcg ccgcctaccc gatcaatttc attccgcacg
     1801 ccagcgacac cgggcgcgcg ccgcatccca ccaacgtcat catgctgacg gcggatgcgt
     1861 tcggcgtcat gccgcccatc gccaaactgt cgcccagcca ggcgatgtat catttcctct
     1921 ccggcttcac ctccagggtc gccggcacgg aaaagggctt gggggcggag ccggagccga
     1981 ccttctccac ctgtttcggc gcccccttca tgccgcgcca tccctcggtc tacggcaatc
     2041 tgctgcgcga cctgatcgac cgctacgatt gcgactgctg gctggtcaac accggctgga
     2101 ccggcggacc ggcggtgcgc gggggcaagc gcatgccgat caaggtgacg cgcgcgctgc
     2161 ttgccgcggc gctcgacggc tcgctggcgg aagcggagtg gcgcgtcgat ccgtttttcg
     2221 gcttccgcgc accggtggcg gtgaagggcg tggatccggc gatcctcgat ccgcgcaaca
     2281 cctggagcaa caaggccgct tacgacacga cggcgaagag gctggccggc atgttccgcg
     2341 acaatttcaa gacctacgtg ccctatgtgg acgagcgggt gctgaaggcc gcggatgtca
     2401 tcgtcaaggc ggcgggatag gattttcaac acaaccaaaa gctgatggtc cgcagcgctg
     2461 ggctaggcgt ttttataagc tggatcaagc ggttgttttt ttgcgtataa gtattgtttt
     2521 taatatataa caatcttaaa ttgtattttt ggcaaccaca ccattgggtg aaatcatgcg
     2581 attaaacgga atagcggccg cggcggccgc gctattggtt ttgggccaag gcgctctggc
     2641 cttcggcacc atccatggcc tcggccagaa tgccgaacac gagcgcatca cccgccacgc
     2701 cctggcctgc ggggcgagcg ggctcagtcc ggcgcaatgt tttcagccgt tttcgatcga
     2761 ccagctggcg ggcaagagcg ggaccttcgg cgctgtcggc gcacccgaca atcccatccg
     2821 cggcctgatg gacaagatct acgcccattg cgacaacggt gatttcatgg acaagcccgg
     2881 ctatccgcaa agcaagacgg ccgcccggca gaatctggcc agctgccgga gctggatgat
     2941 caccaacatg aacgcggcag tggccgattc ggcggcgatg ctgaagaacg gcaagatcga
     3001 cgactcgcag atccccacct acatctcctg caccttcaac ggcacgaaag gccgcgccaa
     3061 atgcaacgcc ctcgaggatt tcgggctgac gctgcacgct tcgcaggatt tctatgccca
     3121 cagcaactgg accgatcatg ccgcgccgca gccgatctcg atcaccaatg ttcccggcct
     3181 cgccaacagc gtgcccgcgc cctggatcga ccccagcctt tccgtgccct tcccggaggg
     3241 actgatgacg ggctgcttca tccag
//
LOCUS       DASWPU010000349         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_113279,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000349 DASWPU010000000
VERSION     DASWPU010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113279"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /gene="rpsH"
                     /locus_tag="VGC27_07485"
     CDS             <1..377
                     /gene="rpsH"
                     /locus_tag="VGC27_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY0282448.1"
                     /translation="DLLSRIRNGQLRGLAKIKSPNSRLRARLLDVLQAEGFIRGYAEV
                     EFKTGMRELEIELKYHEGHPVIRELKRVSTPGRRVYASVKELKPHRQGLGLSIVSTPQ
                     GVMTDTAAREKNVGGEVLCQVF"
     gene            387..920
                     /gene="rplF"
                     /locus_tag="VGC27_07490"
     CDS             387..920
                     /gene="rplF"
                     /locus_tag="VGC27_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY0282449.1"
                     /translation="MSRIGKKPVALPKGVTASVDGKTVKVKGPKGEMMVTLVQEIDAS
                     VGADGVTLTPRKDMERAAQMWGLSRTLVNNLVQGVTSGFTEKLEIQGVGYRAAVQGKS
                     LNLQLGFSHDVAYPIPDGITIAAEKPTMLTISGIDKQLVGQVAAEIRAYRPPEPYKGK
                     GVRYAKEYVRRKEGKKK"
     gene            923..1273
                     /gene="rplR"
                     /locus_tag="VGC27_07495"
     CDS             923..1273
                     /gene="rplR"
                     /locus_tag="VGC27_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY0282450.1"
                     /translation="MTKNLFDRRKKRTRFRLVRNASGRPRLSVFRSGQHIYAQIIDDT
                     EGATLAVASTNEKEAKVAKSWNVEAAVAVGKKIAERAIAKGVKKVVFDRGGYIYHGRV
                     KALADAAREGGLEF"
     gene            1289..1849
                     /gene="rpsE"
                     /locus_tag="VGC27_07500"
     CDS             1289..1849
                     /gene="rpsE"
                     /locus_tag="VGC27_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY0282451.1"
                     /translation="MAREGRERGDREERDSEFVDKLVHINRVAKVVKGGRRFGFAALV
                     VVGDQRGRVGFGHGKAREVPEAIRKATDAAKRALIRVPLKDGRTLHHETRGHHGAGKV
                     LVRPAPAGTGIIAGGPMRAVFEALGVGDVVSKSLGTSNPYNMVRATFDALKHQQSPRM
                     VAQRRGRSVAEIVSRREGQKGAEATA"
     gene            1861..2067
                     /gene="rpmD"
                     /locus_tag="VGC27_07505"
     CDS             1861..2067
                     /gene="rpmD"
                     /locus_tag="VGC27_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009817524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY0282452.1"
                     /translation="MAEKKTAAKKTLTVRQIRSANRKPAIQTATLKGLGLGKMHRMRT
                     LEDTPAVRGMIRAVARLVEIVEEK"
     gene            2127..>2378
                     /gene="rplO"
                     /locus_tag="VGC27_07510"
     CDS             2127..>2378
                     /gene="rplO"
                     /locus_tag="VGC27_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY0282453.1"
                     /translation="MKLNEIRNNPGAHKRKAKLGRGSSSGLGKTSGRGVKGAKARTGT
                     KVYGFEGGQMPLHMRMPKRGFINVFAKDFAEVNLGRIQKA"
BASE COUNT          494 a          693 c          809 g          382 t
ORIGIN      
        1 gcgatcttct gtcgcgcatc agaaacggcc agcttcgcgg cttggcgaag ataaagtcgc
       61 cgaactcgcg gttgcgcgcg cggctgctcg acgtcttgca ggccgaaggc ttcattcgcg
      121 gttacgcgga ggtcgagttc aagaccggca tgcgcgagct cgagatcgaa cttaaatatc
      181 acgaaggcca ccccgtcatt cgcgagttga agcgcgtctc gacgccgggc cgccgtgtct
      241 atgcctcggt gaaggaattg aagccgcatc gtcagggact cggcctgtcc atcgtttcga
      301 cgccgcaggg cgtgatgacg gataccgccg cgcgggaaaa gaacgtcggc ggcgaagtcc
      361 tctgccaggt gttttaggag aaaacgatgt cgcgtatcgg caagaaaccg gttgcattgc
      421 cgaaaggggt tacggcctct gtcgatggca agaccgtgaa ggtcaagggc cccaaaggcg
      481 agatgatggt gacgttggtc caggagatcg acgccagcgt cggcgccgac ggcgttacct
      541 tgacgccgcg caaggacatg gaacgtgccg ctcaaatgtg gggcttgtcg cgtacactgg
      601 tgaataatct ggtgcagggc gtgaccagcg gctttacgga gaaactggaa atacagggcg
      661 tgggctatcg cgccgccgtg cagggcaaga gcctcaacct ccagctcggc ttcagtcacg
      721 atgtggccta tccgattccg gacggcatca cgattgcggc cgaaaagccg acgatgctga
      781 cgatctccgg catcgacaag caattggtcg gtcaggttgc cgcggaaatc cgcgcttacc
      841 gcccgccgga gccctacaag ggcaagggcg tgcgttatgc caaggaatat gtccgccgca
      901 aggaaggcaa gaagaagtag ccatgacgaa gaatctgttc gatcgccgta agaagcgcac
      961 ccgctttcgt ctcgtgcgca acgcgtccgg ccgtccgcgg ctttcggtgt tccgctcggg
     1021 ccagcacatc tatgcgcaga tcatcgacga cacggaaggt gcgacgctgg ccgtcgcttc
     1081 gaccaacgag aaggaagcga aggtcgcaaa gtcctggaac gtcgaggccg ccgtggcggt
     1141 gggcaagaag atcgccgaac gggcgatcgc caagggcgtc aagaaggtcg tgttcgaccg
     1201 tggcgggtac atctatcatg ggcgcgtgaa agcgctggcg gatgccgctc gcgagggcgg
     1261 tttggaattc taatggaagg aacggcacgt ggcgcgtgaa ggcagagaac gcggcgatcg
     1321 cgaagagcgc gacagcgaat tcgtcgataa gctggttcac atcaaccgtg tggccaaggt
     1381 ggtgaagggc ggccggcgtt tcggcttcgc ggcgctcgtc gtcgtcggag atcagcgcgg
     1441 gcgcgtcggc ttcggccacg gcaaggcgcg cgaagtgccg gaagcgatcc gcaaggcgac
     1501 ggatgccgcc aagcgcgcgc tgatccgcgt accgctgaag gacggccgca cgctgcatca
     1561 cgaaacccgc ggccatcacg gcgccggcaa ggttttggtg cgtcccgctc cggccggtac
     1621 cggcatcatc gccggcggcc cgatgcgcgc cgtgttcgaa gcgctgggcg tcggcgacgt
     1681 ggtgtcgaaa tcgttgggaa cctccaaccc ctataacatg gtgcgcgcca cgttcgatgc
     1741 gctgaagcat caacagtctc cgcggatggt ggcccagcgc cgcggccgct ccgtggccga
     1801 gatcgtctcg cgccgcgaag gtcaaaaggg cgctgaggcg accgcataag gaaccggacg
     1861 atggccgaga agaaaaccgc agcgaagaag accctgaccg tgcgccagat cagaagcgcc
     1921 aaccgcaagc ccgccatcca gacggcgacg ctgaaggggt tgggcctggg caagatgcac
     1981 cgtatgcgta cgctggagga cacgcctgcg gtccgcggca tgatccgcgc ggtggcgcgc
     2041 ctcgtcgaga tcgtcgaaga aaaatagatg gatggccggg tcgcgcttcg ctgacgctcg
     2101 ctgcccggcc atgacaagag gttaaaatga agctgaatga aatccgcaac aacccgggcg
     2161 cgcataagcg caaggcgaag cttgggcgcg gttcgtcctc cggcctgggc aagacctccg
     2221 gccgcggcgt caagggtgcc aaggcacgca ccggtacgaa ggtttatggc tttgaaggcg
     2281 gccagatgcc gctgcacatg cgcatgccga agcgcggctt cattaacgtc ttcgccaaag
     2341 acttcgcgga agtgaatctc ggccggatcc agaaggcg
//
LOCUS       DASWPU010000350         3836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_76174,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000350 DASWPU010000000
VERSION     DASWPU010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76174"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..891
                     /locus_tag="VGC27_07515"
     CDS             76..891
                     /locus_tag="VGC27_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY0282454.1"
                     /translation="MLSVNTNPGALTALQYLNSTNAQLQATGSAINSGLKVASAKDNG
                     AIFAIAQNMRGNVAGYSAVSDSLNRASSVVDTGLSAAQSVSDLLIQMKQNALAAADTS
                     LDATSRASLAQDFQALSDQITTIVENASFNGINIVNGAGAGVSALASANDPDQTISVA
                     AQDLTVAELDVDAAYTDQATAKTMVGTVSTAIATVNTALSALSSGSKKFSIQASFVSK
                     LSDTLTSGIGNLVDADMARESALLTALQTKQQLGVQALAIANAAPQTILSLFR"
     gene            complement(1044..2282)
                     /gene="pseC"
                     /locus_tag="VGC27_07520"
     CDS             complement(1044..2282)
                     /gene="pseC"
                     /locus_tag="VGC27_07520"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR03588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HEY0282455.1"
                     /translation="MSLSALKRPPAEPFLPYGRQVIDEDDIRAVCEVLRGDYLTTGPA
                     VGRFETAFANTVDARHAVVCANGTAALYLAARALGLGPGTKVIVPSLTFLATASAPHL
                     SGAEIIFADVDPDSGLMRPQDLAEAIGRGGRGGRADAVFNVHLNGQCGALEEIADVAR
                     ANGLKIVDDACHALGAAYIAKYGTGTLIGSNAFCDLSVFSFHPVKAIAMGEGGAVTAN
                     DPDIAARLGRARNHGLTREAQDFANTREGFEAEGCPNPWYYELVEPGFNWRANDIQCA
                     LGLSQLAKLDRFLARRRALAAVYDELLAPYAPRLKPVARHENCLPAWHLYAVQIDFPS
                     FGLSRAAFMRALAEERIGSQVHYFPLHRQRYYAERYGVAFLPGADRYYARVLSLPFFA
                     SMNEDDVARVVRAMAKILGL"
     gene            complement(2279..3376)
                     /gene="pseB"
                     /locus_tag="VGC27_07525"
     CDS             complement(2279..3376)
                     /gene="pseB"
                     /locus_tag="VGC27_07525"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HEY0282456.1"
                     /translation="MAQRAKKIYLAAENENSIAGPAAPALRDFTQPWLDLNDKAVLIT
                     GGTGSFGKHFVRMVINQYKPRRIVIFSRDELKQYDMQQAFPMEQHPFMRYFIGDVRDR
                     DRLEMAMRDVDYVIHAAALKQVPTAEYNPFECIRTNVFGAENVVSAALRRNVRKVIAL
                     STDKAANPVNLYGASKLASDKIFVAANNLAGADGTRFAVVRYGNVAGSRGSVIPFFKK
                     LADNGASELPITDPRMTRFWITLTQGVNFVLSSMELSRGGEIFVPKIPSTTITGLASL
                     IGPALAQKVVGIRPGEKLHETMIPADDSHWTVELDDRYVILANFAAAAREAYLNRGAK
                     PVSEGFCYSSDLNPDMLDARGLQQLLHEAYA"
     gene            complement(3432..>3836)
                     /locus_tag="VGC27_07530"
     CDS             complement(3432..>3836)
                     /locus_tag="VGC27_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282457.1"
                     /translation="WGKGSLRLGHLTLLPDAFDWSRLSLTTHNGGKDPETFALAGETV
                     DHGAPVSFLVSASHGLAMTEGWAEIGDDRHRLRIEVDRGTAPLLGLLTHKRLRGGVFC
                     QLTLSALELDDTRKPAALHPGPRRFRFSVSAA"
BASE COUNT          712 a         1202 c         1264 g          658 t
ORIGIN      
        1 ttgcctttgg atgaagcggg gcaaaacgcc ctcgagcaaa acgctcgcaa cacaaaagcc
       61 agaacggagg cacttatgct aagcgtcaac accaatccgg gcgcattgac cgccctgcaa
      121 tatctgaaca gcacgaatgc acaacttcag gcgaccggaa gcgccatcaa ctccggcctg
      181 aaagtggctt cggcgaagga caacggcgcc atcttcgcca ttgcccagaa catgcgcggc
      241 aacgtggcgg gctattccgc cgtcagcgat tcgctgaacc gcgcctcctc ggtggtcgat
      301 accggcctgt cggctgcaca gtccgtgtcc gaccttctga tccagatgaa gcagaacgcg
      361 ctggccgcgg ccgacacgtc gctcgacgcg acgagccgcg cctcgctggc ccaggacttc
      421 caggcattgt ccgaccagat caccacgatc gtcgagaacg cgtcgttcaa cggcatcaac
      481 atcgtcaacg gcgccggcgc gggggtcagt gccttggcgt ccgccaacga ccccgaccag
      541 acgatctcgg ttgccgctca ggacctgacg gttgcggaac tggacgtgga cgcggcctat
      601 acggatcagg ccaccgcgaa aacgatggtc ggcacggtct ccacggcgat cgccaccgtg
      661 aataccgccc tgtccgcttt gtcgagcggt tcgaagaagt tctccatcca ggccagcttc
      721 gtctccaagc tgtccgacac gctgacgagt ggcatcggca acctggtcga cgccgacatg
      781 gccagggaga gcgcgttgct gacggccttg cagaccaagc agcagctcgg cgttcaggct
      841 ttggcgatcg ccaacgcggc gccgcagacg atcctctcgc tgttcaggta agtgcatctt
      901 aaggcggcgg gcgccttggc gctcgccgta gcttttccga acggcgagcg ccggcagtta
      961 cggctcttgc cccaggcgtg ggtttggggg ttcgttcaaa aaaagacggc gggcggaggc
     1021 gaaagcctcc gtccgttgcc gttttagaga cccaggatct tcgccatcgc ccgcaccacg
     1081 cgcgcgacgt cgtcttcgtt catcgaggcg aagaacggca ggctgaggac gcgcgcgtaa
     1141 tagcggtcgg cgccgggaag gaaggccacg ccatagcgtt cggcgtaata gcgctggcga
     1201 tgcagcggaa aataatgcac ctggctgccg atccgctctt cggccaaagc gcgcatgaag
     1261 gcggcgcggc ttaggccgaa cgaaggaaaa tcgatctgca ccgcataaag gtgccaggcc
     1321 ggcagacagt tttcatgccg tgcgaccggc ttcagccgcg gcgcataagg cgccagcagc
     1381 tcgtcataca ccgcggccag ggcgcggcgg cgggccagga aacggtcgag cttggcaagc
     1441 tgcgacagcc ccagcgcgca ctggatgtcg ttggcgcgcc agttgaagcc gggttccacc
     1501 agttcgtaat accaggggtt cggacagcct tcggcctcga aaccctcacg cgtattggcg
     1561 aagtcttgcg cctcgcgcgt caggccgtga ttgcgcgccc gcccgaggcg ggcggcgata
     1621 tccggatcgt tcgccgtcac cgcgccgcct tcgcccatgg cgatggcttt gaccgggtgg
     1681 aaggagaaca cgctgagatc gcagaaggcg ttgctgccga tcaaggtgcc ggtgccgtac
     1741 ttggcgatat aggcggcgcc cagcgcatgg caggcatcgt cgacgatctt gaggccattc
     1801 gcccgcgcga cgtccgcgat ttcctccagc gcgccgcatt ggccgttgag atggacgttg
     1861 aaaaccgcat cggcgcggcc gccgcggccg ccgcggccga tggcttcggc gagatcctgc
     1921 gggcgcatca ggccgctgtc gggatcgaca tcggcaaaga tgatttcggc gccgctaaga
     1981 tggggcgcgc tcgctgtggc caggaaggtc agcgagggca cgatgacctt ggttcccggc
     2041 cccaagccga gggcgcgcgc cgccaggtac aaggccgccg tgccgttggc gcagaccaca
     2101 gcatgtctgg catcgacggt gttggcgaag gcggtttcga agcggccgac ggccggcccg
     2161 gtggtcaggt aatcgccgcg cagaacctcg cagacggccc ggatatcgtc ctcgtcgatg
     2221 acctgccggc cataaggcag aaaaggctcc gccggcggcc gctttagcgc cgagaggctc
     2281 acgcgtaggc ctcatgcaga agctgctgca agccgcgcgc gtcgagcatg tccgggttga
     2341 ggtcgctcga atagcagaag ccctcgctca ccggcttggc gccgcgattg agataggctt
     2401 cgcgcgccgc cgcggcaaaa ttggcaagga tcacatagcg gtcgtcgagt tcgacggtcc
     2461 aatgcgaatc gtcggccgga atcatcgtct cgtgcagttt ctcgccgggg cggatgccga
     2521 cgaccttctg agcgagcgcc ggtccgatca gactggccag cccggtgatc gtggtggagg
     2581 gaatcttcgg cacgaaaatc tcgccgccgc gcgacagctc catgctcgaa agcacgaagt
     2641 tcacgccttg ggtgagcgtg atccagaacc gcgtcatccg ggggtcggtg atcggcagct
     2701 cgctggcgcc gttatccgcc aatttcttga agaagggaat gaccgagccg cgcgagcccg
     2761 caacgttgcc gtagcgcacc acggcgaagc gggtgccgtc ggcgccggcc agattattgg
     2821 cggcgacgaa aatcttgtcc gacgccagct tggaggcgcc gtagagattg accgggttgg
     2881 cggctttgtc ggtcgatagc gcaatcacct tgcgcacatt gcgcctgagc gccgcggaaa
     2941 ccacgttctc ggcgccgaag acgttggtgc ggatgcactc gaagggattg tattcggcgg
     3001 tgggaacctg cttcagcgcg gccgcatgga tgacgtaatc gacgtcgcgc atcgccattt
     3061 cgaggcggtc gcggtcgcgc acgtcgccga tgaagtagcg catgaacgga tgctgctcca
     3121 tcgggaaggc ttgctgcatg tcgtattgtt tgagctcgtc gcgcgagaag atgacgatgc
     3181 ggcgcggctt gtattggttg atcaccatgc gcacgaaatg tttgccgaac gatcccgttc
     3241 cgccggtgat cagaacggcc ttgtcgttga ggtcgagcca gggctgggtg aagtcgcgca
     3301 aggccggggc cgcaggcccg gcgatgctat tctcgttctc ggccgccaga tagattttct
     3361 tcgcccgctg cgccatggac ccgccccgaa tttgccgaaa tcatggttac agaatgtggt
     3421 aaagattcgg tctatgccgc gctcacgctg aagcgaaagc gccgcgggcc ggggtgcagc
     3481 gcagcgggtt tgcgcgtgtc gtccagctcc agggccgata gcgttaactg gcagaacacg
     3541 ccgccgcgaa gacgtttatg ggtcagaagc ccgagcagcg gggcggtgcc acgatccacc
     3601 tcgatcctga gccggtgcct gtcgtcgccg atttccgccc agccttcggt catcgccaag
     3661 ccgtgcgagg cggagaccag gaacgatacc ggtgcgccgt gatcgacggt ctcgcccgcc
     3721 agggcaaaag tttcggggtc cttgccgcca ttgtgcgtcg ttaacgaaag cctgctccaa
     3781 tcgaaggcgt ccggcagaag cgtgagatgg cccagccgca agctgccctt gcccca
//
LOCUS       DASWPU010000351         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_57764,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000351 DASWPU010000000
VERSION     DASWPU010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57764"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..>2554)
                     /locus_tag="VGC27_07535"
     CDS             complement(17..>2554)
                     /locus_tag="VGC27_07535"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY0282458.1"
                     /translation="NVTGRLHIGHALNNTLQDALARFERMRGKDVLWQPGMDHAGIAT
                     QLVVERQLAERQQSRVAMGREKFLEAVWKWKDESGGAIGEQLRRLGASADWSRERFTM
                     DEGLSRAVLKVFVELYRQKLIYKDKRLVNWDPRLQTAVSDLEVESIELKGHLWYLKYP
                     VEGQPGRFITVATTRPETMLGDTAVAVHPGDERYKALVAAKAQVRLPLTGRLIPIIAD
                     EHCDPEKGTGAVKITPGHDFDDFEVGRRHNLPLINILNKDGTLNDTVPAYRGMDRFVA
                     RKAVVADLEALGLLDKIESIVHAVPHDEKTKTVVLEPYMTEQWYLDVSTLAERAIKAV
                     EDGRTKFVPENWANVYFNWLRHIQPWCISRQLWWGHQIPVWYDDEGNVFVADSEAEAQ
                     KQAGGKKLTRDPDVLDTWFSSALWPFSTLGWPDETKEVKRYYPTSVLITGFDIIFFWV
                     ARMMMMGLHFMDDVPFRTVFIHTRVLDEKGVKMSKTKGNVVDPLELIDAYGADALRFT
                     LALAAGQGRDMRIGPSRVETNRNFATKLWNAARFCEMNGCDTRADFDPAKVSHTVNRW
                     IAAETARTAAEVTAHLETLRFNDAAGAAYHFVWDVYCDWYLEFIKPLLNGTDGAMSDS
                     ELRAKAEARMTAAWVRDQILKLLHPFMPYITEELWARTAEHGEKRASLLIEAEWPQWT
                     ALPNNEDASTEMRWVVDLISGVRSIRAEMNVPPSAKIALVLKDASIATRARLERHHGV
                     ILTLARLSSANIADAIPQGSAQFVVGEAVAALPLGDVIDFAKERARLEKELKKAHDEI
                     ARFDAKLANEQFVSRAPEDVLAEQREKRTEAAALAARLKEAMGRLTK"
BASE COUNT          413 a          848 c          783 g          510 t
ORIGIN      
        1 tgcgcctcct cgcccgtcat ttcgtcaatc gccccatcgc ttccttcagc ctggccgcca
       61 gcgcggccgc ttccgtccgc ttctcgcgct gttcggcaag gacgtcctcc ggcgcgcggg
      121 agacgaactg ctcgttggcg agcttggcgt cgaagcgggc aatctcgtcg tgcgccttct
      181 tcagttcctt ctccagccgc gcgcgctcct tggcgaagtc gatcacgtct ccgagcggca
      241 gcgccgccac cgcctcgccg acgacgaatt gcgccgagcc ttgcggaatc gcgtccgcga
      301 tgttcgcgga agacagtcga gccagcgtaa gaatgacacc gtgatggcgt tccagccgcg
      361 cgcgcgtcgc gatgcttgca tccttcagga ccagagcgat cttggccgac ggcggcacgt
      421 tcatctcggc gcggatcgag cgcacgccgg aaatcagatc gacgacccac cgcatttccg
      481 tgctggcgtc ttcgttgttg ggcagcgccg tccattgcgg ccactccgct tcgatcagca
      541 gcgaggcgcg cttctcgcca tgttccgccg tccgcgccca aagctcctcg gtgatgtagg
      601 gcatgaacgg atgcagcagt ttcagaatct ggtcacgcac ccaggccgcc gtcatgcgcg
      661 cttcggcctt ggcgcgcaat tcgctgtcgc tcattgcgcc gtcggtgccg ttcagcagcg
      721 gcttgatgaa ttcgagatac cagtcgcagt aaacgtccca gacgaaatga taggccgcgc
      781 ccgccgcatc attgaagcgc agcgtttcca gatgggccgt cacctcggcc gccgtgcgcg
      841 cggtctcggc cgcgatccac ctgttgaccg tgtgcgacac cttcgccgga tcgaaatccg
      901 cgcgcgtgtc gcagccgttc atctcgcaga agcgcgccgc gttccacagc ttggtcgcga
      961 aattgcggtt ggtctcgacg cggctcggcc cgatgcgcat gtcgcggccc tgccccgccg
     1021 ccagcgccag cgtgaagcgc aaggcgtccg cgccataggc atcgatcaat tccagcggat
     1081 cgacgacgtt tcccttggtc ttcgacatct tcacgccttt ttcgtcgaga acgcgggtgt
     1141 ggatgaagac ggtgcggaac ggcacgtcat ccatgaaatg cagacccatc atcatcatcc
     1201 gggcgaccca gaagaagatg atgtcgaagc cggtgatgag gacgctggtg ggatagtagc
     1261 gcttgacctc tttggtctcg tcgggccagc ccagggtcga gaacggccat agtgccgaag
     1321 agaaccaggt gtcgagcacg tcgggatcgc gcgtcagttt tttcccgccc gcctgctttt
     1381 gcgcttccgc ttcgctgtcg gcgacaaaga cattgccttc gtcgtcgtac cagaccggaa
     1441 tctgatgccc ccaccagagc tggcgcgaaa tgcaccaagg ctggatgtgg cgcagccagt
     1501 tgaaatagac gttggcccag ttctccggca cgaatttcgt gcgcccgtct tccacggcct
     1561 tgattgcgcg ttccgctagg gtcgaaacgt cgagatacca ttgttcggtc atgtagggtt
     1621 cgagcacgac tgttttagtc ttctcgtcgt gcggcacggc gtggacgatg gattcgatct
     1681 tgtcgagcag gcccagcgct tccagatcgg cgaccaccgc cttgcgcgcc acgaagcgat
     1741 ccatcccgcg ataggccggc accgtatcgt tcaaggtgcc gtccttgttc aggatgttga
     1801 tcagcggcag gttgtgccgc cggccgacct cgaaatcgtc gaaatcgtgg cccggcgtga
     1861 tcttcaccgc gccggtaccc ttttcggggt cgcaatgttc gtcggcgatg atcgggatca
     1921 gccgtccggt cagcggtaag cgaacctgcg ccttcgccgc gaccagcgcc ttgtagcgct
     1981 cgtcgccggg gtgaaccgcc acggcggtgt cgccgagcat ggtttcgggc cgggtcgtgg
     2041 cgacggtgat gaaccggccc ggctgcccct cgacggggta tttgaggtac cagaggtggc
     2101 ccttgagctc gatgctctcg acttcgaggt cgctgaccgc cgtctgaagc cgcgggtccc
     2161 agttgaccag ccgcttatcc ttatagatga gcttctggcg ataaagctct acgaacactt
     2221 tcaaaaccgc ccgcgacagc ccctcgtcca tggtgaagcg ttcgcgcgac cagtcggccg
     2281 aagcgcccag ccggcgcaat tgctcgccga tggccccgcc ggattcgtcc ttccacttcc
     2341 acaccgcctc caggaacttc tcccggccca tggcgacgcg gctctgctgg cgttcggcaa
     2401 gctggcgctc cacgacaagc tgggtggcaa tgccggcatg gtccatgccc ggctgccaca
     2461 gcacgtcctt gccccgcatc cgctcgaaac gggccagggc gtcctgcaac gtgttgttga
     2521 gggcgtggcc gatgtgcagc cgtccggtga cgtt
//
LOCUS       DASWPU010000352         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_100928,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000352 DASWPU010000000
VERSION     DASWPU010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100928"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..2131)
                     /locus_tag="VGC27_07540"
     CDS             complement(53..2131)
                     /locus_tag="VGC27_07540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEY0282459.1"
                     /translation="MDPMEAIKQTYFQECDELLLAMEEGLIAMENGEADGEVINAVFR
                     AVHSIKGGGGAFGFEALVGLAHVFETVLDQVRSGTLSPSGDAVKLLLRAGDKLSDHVA
                     AARNGGGEVPDSDVKDELNELAGVSSEDAAPAEMEGVPFTPIQIDIADEPTFSPSPGS
                     WKIHFAPHAALYAKANEPFLLVRELARLGSIEVGADISRVPELAAIEPDQAYFAWDIT
                     LRGCNNRQEIEEVFEFVSGDCDLEISENTPPASLGVGDETPSAGMQAAPAAPAETAPA
                     EKKATSPAPAEAVKQTIRVDLDKVDRLVNLVGELVITQAMLSQRVAECNSGRGTTLGA
                     GLNELEHLLRELQEGVMAIRTQPVKSVFQRMPRLVRELAAQTNKQVHLVLEGEATEVD
                     KTVIERLGEPLTHMIRNAIDHGLETTEDRLAAGKPAEGTVTLSAEHRGGRIVIEVADD
                     GRGIDRQRVRKRAVEKGLISPSACLSDEEIDNLIFLPGFTTASQVSNISGRGVGLDVV
                     RRNIVDLGGRIVIGSTAGKGSRFSLTLPLTLAVLDGMVVAVGNQTFVLPLSHIVESLL
                     PKASDIRPLGTNGRLLNVRGTYVPLASIGELLGVEGALKDSAEGVVILVESEGVGRMA
                     LGVDAIVGQRQVVIKSFESNFRHIDGIAAATILGDGRVALILDTDGLVARCREAAISR
                     QREYQQAIGA"
     gene            complement(2141..2509)
                     /locus_tag="VGC27_07545"
     CDS             complement(2141..2509)
                     /locus_tag="VGC27_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0282460.1"
                     /translation="MGKIILTVDDSRTMRDMLRLALCDAGHTVIQANDGVHGAEVLQS
                     AESLDVIITDINMPRMDGYEFIEHVRRDSSYRATPILVLTTESEPAKKLRARDAGATG
                     WIVKPFDPDKLLAAIRRVSP"
     gene            complement(2512..>2796)
                     /locus_tag="VGC27_07550"
     CDS             complement(2512..>2796)
                     /locus_tag="VGC27_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0282461.1"
                     /translation="GNGSDGRITLPAVVDLLQAGMLKAQLERGLASGCGMTITASAVQ
                     RVSSPCLQVLAAGVSAFEKAGGPSLTIADPSPAFLETVSMLGLTDALRLA"
BASE COUNT          468 a          896 c          872 g          560 t
ORIGIN      
        1 gtgatgtact ggcggcggtc ttgcactgtt tcttgctggt tgtggctcat ggctacgctc
       61 cgattgcctg ttgatattct ctttgccggc tgatggcggc ttcgcgacag cgcgccacca
      121 ggccgtccgt atccagaatg agggccacgc ggccgtcgcc cagaatggtt gccgcggcga
      181 tgccgtcgat gtggcggaaa ttcgactcga aactcttgat gacgacttgg cgctggccga
      241 cgatcgcatc gacgccgagc gccatgcgcc cgacgccctc gctttccacc aggatgacca
      301 cgccctcggc ggaatccttc agggcgcctt ccacgcccag caactcgccg atgctcgcca
      361 acggcacgta agtgccgcgt acgttgagga gccgtccgtt cgttccgagc gggcgaatat
      421 cggatgcctt gggcagaagg ctttccacga tgtgcgacag cggcaggacg aaggtctggt
      481 ttccgaccgc cacaaccatt ccatccagga ccgccagggt cagcggcagc gtaaggctga
      541 agcggctgcc tttgccggcc gtcgaaccga tgacgatgcg cccgccgaga tcgacgatgt
      601 tgcggcgcac gacatccagg ccgacaccac ggccggagat gttcgagacc tggctggccg
      661 tcgtgaaacc ggggagaaag atgagattgt cgatttcctc gtcggatagg caggcggaag
      721 gggagatcag acccttctcg acggccctct ttcgcacgcg ctggcggtcg atcccgcggc
      781 cgtcgtcggc gacttcaatg acgatccgcc cgccccggtg ttcggccgac agcgtaaccg
      841 tgccttcggc cggttttccg gccgccagac gatcttccgt tgtttccaac ccgtgatcga
      901 tggcgttgcg gatcatgtgc gtcagcggct cgccgagccg ttcgatgacc gtcttgtcga
      961 cttcagtggc ttcgccctcg agaacaaggt gaacctgctt gttggtttgc gccgccaatt
     1021 cgcggacgag cctgggcatg cgctggaaca ccgatttgac cggctgggtg cgaatagcca
     1081 tgacgccttc ctgcaattcg cgcagcaggt gttcgagttc gttgaggccg gcccccagcg
     1141 tcgttcctcg ccccgaattg cactctgcga cgcgctgcga cagcatggcc tgggtaatca
     1201 ccagctcgcc gacgagattc accagccgat cgaccttgtc gagatcgaca cggatggtct
     1261 gcttcaccgc ctccgcgggc gcaggactcg tggctttctt ctctgcgggc gctgtttcgg
     1321 ccggcgcagc aggtgctgct tgcattccgg ccgatggggt ttcgtccccg actcccaggc
     1381 tggccggcgg cgtgttttcg ctgatctcca ggtcgcaatc cccggagacg aattcgaaga
     1441 cctcctcgat ctcctggcgg ttgttacagc cgcgaagcgt gatgtcccat gcgaagtaag
     1501 cctggtccgg ttcgatcgcc gcaagttccg gaactctcga tatgtccgct ccgacctcga
     1561 tcgatccgag cctggcgagt tcccggacga gcaggaacgg ctcgttcgcc ttggcgtaga
     1621 gtgcggcatg aggcgcaaaa tgtattttcc atgaacccgg cgaaggcgaa aatgtgggtt
     1681 cgtctgcgat gtctatttgg atgggagtga acggcacgcc ttccatctcc gccggtgcgg
     1741 catcttcgga tgacacgccc gccaactcgt tcaattcgtc cttgacgtcc gaatccggca
     1801 cctcgccgcc gccgttgcgg gcggccgcga cgtgatccga aagcttgtcg ccggccctca
     1861 gcaacagctt cacggcgtcg ccgctgggcg aaagcgttcc ggaacgaacc tggtcgagaa
     1921 ccgtttcgaa aacatgggca aggccgacga gcgcctcgaa cccgaaggcg ccgccgccgc
     1981 ccttgatgga gtgcacggcg cggaacacgg cattgatgac ctcgccgtcc gcttcgccgt
     2041 tctccatcgc gatcaggcct tcctccatgg cgagaagaag ttcgtcgcac tcctggaagt
     2101 aggtttgttt gatggcttcc atcgggtcca tggcgtcgtc ctatggcgaa acgcgacgga
     2161 tggccgcgag caatttgtcg ggatcgaacg gcttgacgat ccagccggtc gcgcccgcgt
     2221 cgcgcgcccg cagttttttc gccggttcgc tttccgtggt gagcaccagg atcggcgtgg
     2281 cgcggtacga agagtcgcgc cggacatgct cgatgaattc gtacccgtcc atgcgcggca
     2341 tgttgatgtc ggtgatgatc acgtccaagg actcggcgct ctgaagcact tcggcgccgt
     2401 gaacgccgtc attggcctgg atcaccgtgt gtcccgcgtc gcacagcgcg agtctgagca
     2461 tgtctcgcat ggtccgggag tcgtcgaccg tgaggattat ctttcccatc gtcacgccag
     2521 cctcaaagcg tccgtcagtc cgagcatcga gacggtctcc agaaacgccg gcgaggggtc
     2581 ggcaatcgtc agcgacggcc cgccggcttt ctcgaaggcg ctgacccctg cggccagaac
     2641 ctgaaggcat ggcgaactga cgcgctgaac ggcggaagca gtgatcgtca tcccgcatcc
     2701 gctcgccagg cccctttcga gttgggcctt caacatgccc gcttgcagga ggtcgacgac
     2761 ggcaggcaga gttatcctgc cgtccgaccc gttgcc
//
LOCUS       DASWPU010000353         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_107121,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000353 DASWPU010000000
VERSION     DASWPU010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107121"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VGC27_07555"
     CDS             <1..422
                     /locus_tag="VGC27_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282462.1"
                     /translation="KLALVRPATDNFAATNWLRRDGDGRAAKDAVGTPAQGVRCDAYG
                     CIAKARGGTVVAVSSRAEALAEDCARADIVVSAVPARRFCNGTALTIDRIDIVRHGAH
                     AVWLGPPLRFETVEGERGRRPWSAPIRTKRTPPNSGG"
     gene            complement(406..1206)
                     /gene="lexA"
                     /locus_tag="VGC27_07560"
     CDS             complement(406..1206)
                     /gene="lexA"
                     /locus_tag="VGC27_07560"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY0282463.1"
                     /translation="MFRICSPARIPHAGAKGGVAMLTRKQYELLMFIHERVRESGIPP
                     SFDEMKDALDLKSKSGIHRLITALEERGFLRRLEKRARALEIIKLPDNVAETLRPATS
                     RSQVMRMSPTRHTRGEARVSSDTRGAGSPRRPIGEGEGAINVPLVGRIAAGTPIEALQ
                     NKMADVPVPSGMVGRGDHYALEVTGDSMINAGILDGDTVIIQEVDTANTGEIVVALVD
                     NTEATLKRLRRRGDSIALEAANPAYETRLYGADRVRVQGKLVGLIRRY"
     gene            complement(1223..1633)
                     /locus_tag="VGC27_07565"
     CDS             complement(1223..1633)
                     /locus_tag="VGC27_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282464.1"
                     /translation="MFDLYTDDIASHSPSLNLPRPLELRGMDSHLTPAECAKERAKSK
                     TESAAELCSKKEMWLGTPHKKVDNNYLAGLSGEAGRKAKTLVSEGDNDVLADSNSSLL
                     DRGSERKRRAAILADKSSRSVIGTRFRVLTPTKN"
     gene            complement(1778..2212)
                     /locus_tag="VGC27_07570"
     CDS             complement(1778..2212)
                     /locus_tag="VGC27_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0282465.1"
                     /translation="MRHLALTFALTALATLPARAADRAALEAEVAATETAFAQTMADR
                     DLAAFSRFVSGEAVFFTGPAPLRGKSAIVAYWQRWYTEKQPPFSWRPEKLEVLDSGTL
                     ALSTGPVFDKNGKRVSSYTSIWRLEAPGTWRIVFDKGCGCAD"
     gene            complement(2355..>3087)
                     /locus_tag="VGC27_07575"
     CDS             complement(2355..>3087)
                     /locus_tag="VGC27_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEY0282466.1"
                     /translation="DCALLAAGDIAHAKVRRRPRIAVISIGDELSRPGEERKPGGIVA
                     SSGYGLSAMVHDCGGAPCDFGILQDNEADIATVADADADFIITLGGVSVGDHDLVQKA
                     LGTKNFALEFWRVAMRPGKPLIFGRLGKTPLLGLPGNPVSTLVCGILFVKPAIASMLG
                     TPCEPDVLSAKLVAALPANDARQTYLRAKLTRQNGECCVEAFAVQDSSMLTILAQANA
                     LIVRAPDAPAANAGEKVDIIPLDGH"
BASE COUNT          550 a          973 c         1002 g          562 t
ORIGIN      
        1 gcaagctcgc tctggtgcgc cccgcgacgg ataacttcgc cgccaccaac tggctgcggc
       61 gcgacggcga cggccgtgcc gcgaaagacg ccgtgggaac gcccgcgcaa ggcgtgcgct
      121 gcgacgctta cggctgcatc gccaaggcgc ggggcggaac ggttgtggcg gtgtcgtcgc
      181 gcgccgaggc gctggccgag gactgcgccc gcgcggatat cgtggtgagc gccgtgccgg
      241 cgcggcgctt ctgcaatggg acggcgctga cgatcgaccg gatcgatatc gtgcgccacg
      301 gcgctcatgc ggtctggctg ggcccgccgc tgcgcttcga aacggtggaa ggcgaacgcg
      361 gccgcaggcc gtggtcggca ccgatccgca cgaaacgaac gccgcctaat agcggcggat
      421 gaggccgacc agcttaccct gcacgcggac gcgatccgcg ccgtaaagcc gcgtttcgta
      481 ggcggggttg gccgcttcga gggcgatgga atcaccgcgc cggcgcaggc gcttgagcgt
      541 cgcttcggta ttatcgacca gcgccacgac gatctcgccc gtattggcgg tatcgacttc
      601 ctggatgatc acggtatcgc cgtcgagaat gcccgcgttg atcatggaat cgccggtgac
      661 ttccagcgcg taatgatcgc cgcggccgac catgccgctg ggcaccggta catcggccat
      721 cttgttctgc aaagcctcga tgggcgtgcc cgccgcgatg cggccgacca gcggcacgtt
      781 gatcgcgcct tcgccttcgc cgatggggcg gcgcggacta ccggcgccgc gcgtgtccga
      841 cgagacccgc gcttcgccgc gcgtgtgccg ggtagggctc atgcgcatga cctggctgcg
      901 cgaggtggcg gggcgcagcg tttcagccac gttatcgggc agcttgatga tttcgagcgc
      961 gcgggcgcgc ttttccaggc gcctgaggaa gccgcgctct tccagcgccg tgatcaggcg
     1021 gtggatgccg gatttggact tcagatccag cgcatccttc atttcgtcga aggagggcgg
     1081 aatgccgctt tcgcgaaccc gttcgtggat gaacatgagc agttcgtatt gcttgcgggt
     1141 gagcatcgcc acgcctccct tggcgccggc atgcgggatt cgcgccggcg aacaaatccg
     1201 aaacatggag aatcgttcta ctttagttct tcgtgggcgt caacacgcga aatctggtgc
     1261 caataacgct tctcgaagat ttgtcggcca agatcgctgc gcgtcgtttc ctttcactcc
     1321 ctcgatccag caaacttgaa ttcgaatcgg ccaaaacatc gttatctcct tcactgacca
     1381 gcgttttcgc cttccttccg gcttcgccgg acaggccggc aagatagttg ttatcgactt
     1441 tcttatgcgg ggtacccagc cacatctctt tctttgagca caattccgcc gccgactccg
     1501 ttttgctttt tgctctttct ttcgcacact cagccggagt gagatgggaa tccattccgc
     1561 gcagttcaag agggcgcggg agatttaatg aaggagaatg ggaagcaata tcatctgtgt
     1621 acagatcgaa cacaatgacg agaacactat accccaatgt cggtcacaac atcgtggatt
     1681 gcgggacgtg cggtgaatag tgctcctgtc actgtagtcg tcagaaagac atccacccgt
     1741 taccccgttt tcgccgccga tggaccatcg gcggcattca atcggcgcag ccgcatccct
     1801 tgtcgaacac gatgcgccat gtgcccggcg cttcaagccg ccagatcgag gtgtagctgc
     1861 tgacgcgctt gccgttcttg tcgaacacgg ggccggtcga gagtgcgagg gtgccggagt
     1921 cgagcacttc cagcttttcg ggccgccagg aaaaaggcgg ctgcttttcc gtgtaccagc
     1981 gctgccaata ggcgacgatc gcactcttgc cgcgcagcgg cgcggggccg gtaaagaaca
     2041 cggcctcgcc ggaaacgaag cgggagaaag cggcaagatc gcgatccgcc atggtctggg
     2101 cgaaggcggt ttcggtggcg gcaacttcgg cttccaacgc ggcgcggtcg gccgcgcggg
     2161 cgggaagggt ggcaagcgcc gtgagtgcaa atgtcagagc caaatgtcgc atcgctacgc
     2221 cccccatcga aaggtgggca gtttagcgcg acggcgatcc accgccagag gcgaaccgca
     2281 agcgtgagtg caaacaccct atccgccttc gcgctaacgc gcttcgacgg gacaagtaag
     2341 ggggggtgat tcaatcaatg cccatccagc ggaatgatat cgaccttctc acccgcattc
     2401 gccgccgggg cgtcgggcgc gcggacgatc agtgcgttgg cttgcgccag gatggtgagc
     2461 atcgaggaat cctgcaccgc gaaggcttcc acacagcatt caccgttctg ccgcgtcagc
     2521 ttggcgcgca gataggtctg ccgcgcgtcg ttggcgggaa gcgcggcgac cagctttgcc
     2581 gacagcacgt ccggttcgca tggcgtgccc agcatcgaag cgatggcggg cttcacgaac
     2641 aagatgccgc agaccagcgt ggacaccgga ttgcccggaa ggccgagcag gggcgtcttg
     2701 ccgagcctgc cgaagatcag cggcttgccc ggccgcatcg cgacccgcca gaactctagc
     2761 gcgaagttct tggtgcccag cgctttctga acgaggtcgt gatcgccgac cgaaaccccg
     2821 cccagcgtga tgatgaagtc ggcgtcggcg tcggccaccg tggcgatgtc ggcctcgttg
     2881 tcctgcagga tgccgaagtc gcacggcgcg ccgccacagt cgtggaccat cgccgataat
     2941 ccgtaccctg acgaagccac gatgccgccc ggcttgcgct cctcgccggg ccgggacaac
     3001 tcgtcgccga tcgaaatgac ggcgatgcgc ggcctgcggc gcaccttcgc atgggcgatg
     3061 tcgcccgccg cgagcagggc gcaatca
//
LOCUS       DASWPU010000354         5900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_94800,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000354 DASWPU010000000
VERSION     DASWPU010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94800"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VGC27_07580"
     CDS             complement(<1..292)
                     /locus_tag="VGC27_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282467.1"
                     /translation="MGKDRAVLEGFRTQPASFRAAWAASSGRTIGAKYLIFVPKTVER
                     PTRLGFSKKLLSRMTTLGPYSVGARYETGYGDLPPRDDAFSSGVSTPARHAGA"
     gene            434..1255
                     /gene="fabI"
                     /locus_tag="VGC27_07585"
     CDS             434..1255
                     /gene="fabI"
                     /locus_tag="VGC27_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY0282468.1"
                     /translation="MAFEGLMKGKRGLVMGVANERSIAWGIAQSLAESGAELAFTYQG
                     EAFKKRVIPLTAPLKPAVVADCDVTNPASLDALFAEIGKAWGGLDFVVHAVAFSDKEQ
                     LKGRYVETTQENFAMTMNISCYSFTAVAQRAEKLMTNGGSLVTLTYYGAEKVMPHYNV
                     MGVAKAALEASVRYLAEDLGKSAIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPL
                     RRTVSQTEVGSAALFLLSDLGKAVTGECLHVDAGYHIIGMKAPDAPDIVVGKNGD"
     gene            1381..2481
                     /gene="aroC"
                     /locus_tag="VGC27_07590"
     CDS             1381..2481
                     /gene="aroC"
                     /locus_tag="VGC27_07590"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY0282469.1"
                     /translation="MSHNIFGHLFRVTTFGESHGPALGCVIDGCPPGIPLTAADIQAD
                     LDRRRPGQSKFVTRRKEPDRVRILSGVFQDERMKSQVTTGTPIALLIENVDARSKDYS
                     DIRDKFRPGHADYTYWAKYGVRDYRGGGRQSARETAARVAAGAVARKVLASLYRGVRV
                     RGALIQMGTQTIERKRWNWNEVPRNPFFCPDVQAIAPWTEYLECVRKKGSSCGAIVEV
                     TAEGIPAGLGAPVYGKLDADIASAMMSINAVKAVEIGDGFATAALSGADNSDDMRAGR
                     GRKPKFLSNHAGGILGGISTGQPIVARFAVKPTSSILTPRKTIDIHGNDTDIVTKGRH
                     DPCVGIRAVPIGEAMLALVLADHALRHRGQMG"
     gene            2509..3426
                     /locus_tag="VGC27_07595"
     CDS             2509..3426
                     /locus_tag="VGC27_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0282470.1"
                     /translation="MDDIIMIILIILAVIALSALMSIVFTVKQQTCAIVERFGRYVRT
                     ARPGLNFKVPFIENIVQRVSLRVQQLIIEVETKTLDNVFVKLIVAVQFRVVQGSEADS
                     YYKLQDHVEQIKSYVLDVVRAKVPKMNLDEVFEKKDDIGSAVKSELDVSMKIYGFEIP
                     NALVTDVNPADNVKAAMNEIQTQRRLQDAATAKGEANKILVVKNAEAEAESKRLQGEG
                     IAKQRRAIVDGLRESIAAFADQIGSVNEHEVLNLVLMTQYFDTLKEIGVSSGSKVILT
                     PHAPGGMTDMTEQLRAAIIQGNEAVAGKK"
     gene            complement(3514..4560)
                     /locus_tag="VGC27_07600"
     CDS             complement(3514..4560)
                     /locus_tag="VGC27_07600"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282471.1"
                     /translation="MTLCLHFGTCGGCAHQGMEDYHAFKRALVTDALARHGIEASVEE
                     IVEVPPATRRRATFKALKGESGVALGFHGARTHDIVDMHECLVLTPRLVALVPNLREM
                     LGTLLRQGEGAELRLADTDTGVDLSLRWQKTGDAATLAGLARWAAKLKLARVARHGET
                     LIELARPSVRLGKAELFVPSEAFLQPTREGEAALQAFVLETLAKAKSVADLFSGCGTF
                     ALPLAGRARVHAVEIEGPMLEALAAAARVTSCLKPITVEKRNLFRRPLNENDLLAFDA
                     VALDPPRAGALEQVRMLARSKLKRVAYVSCDAETFARDARILIDGGYSLTRVQPIDQF
                     LWSNHIELAASFER"
     gene            complement(4680..5423)
                     /locus_tag="VGC27_07605"
     CDS             complement(4680..5423)
                     /locus_tag="VGC27_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY0282472.1"
                     /translation="MITRADVFLVEKGYAASRAEAQAAIRAGHVNADGKPVSKPSQTI
                     PENAVIEYRKPHPYVSRGALKLAAALDRFELSPDDLICIDVGASTGGFTEILLERGAR
                     KVYAVDVGHGQMHAKLAADPRVILLEGVNARDLGPTQVPEQVDAVVVDVSFIGLKLVL
                     PAALKRMRQGAWLVALIKPQFEAGIGKVGKGGIVKDKSVQEEALQGIAGWLGAQLGWS
                     VIGTMESPIAGGDGNREFLLAAKRNPSSP"
     gene            complement(5521..>5900)
                     /locus_tag="VGC27_07610"
     CDS             complement(5521..>5900)
                     /locus_tag="VGC27_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0282473.1"
                     /translation="LAEALVAAERLSAYGLSVTVADARFAKPLDRDLILRLAREHEVL
                     LTVEEGAAGGFGSHVMQFLAWAGCLDKGLKIRPMVLPDRFQNQDSQARMYAAAGLDAN
                     SIAAAALAALGREEEAAAVSQIA"
BASE COUNT         1151 a         1910 c         1826 g         1013 t
ORIGIN      
        1 gagcaccggc atgccgagct ggcgtagata cccccgatga aaaggcatca tcgcgcggcg
       61 gaagatcgcc gtagccagtt tcatatcgtg ccccgacgct gtatggaccc agcgtcgtca
      121 ttctggacaa aagcttttta gaaaatccta agcgtgtggg ccgttcgacg gtcttcggca
      181 caaaaatcag gtacttagct ccaatggttc gaccagatga agccgcccag gcggcgcgaa
      241 atgacgccgg ttgcgtgcgg aaaccctcca aaacagcccg gtccttgccc attccggcca
      301 tttggttgag tgtacggcac gtatatctct ggttgtattc gtattccggc cccctagccg
      361 gcagccatcc tgtttcttga ttttgcttac tttttcgcgc attgggatca cgcaaaccga
      421 cgggagtagc tgcatggcgt tcgagggcct gatgaaaggc aagcgcggcc ttgtgatggg
      481 cgtcgccaat gagcgctcca tcgcttgggg catcgcccaa agtctcgccg aatccggcgc
      541 cgagctggca ttcacctacc aaggcgaagc cttcaagaag cgcgtcatcc cgctgaccgc
      601 accgctgaag ccggccgtcg tcgccgattg cgacgtcacc aacccggcct ccctcgacgc
      661 gctgttcgcc gagatcggca aagcctgggg cgggctcgac ttcgtggttc acgccgtcgc
      721 cttctccgac aaggagcagc tcaagggccg ctacgtcgag accacgcagg aaaacttcgc
      781 gatgacgatg aacatctcct gctattcgtt tacggcggtg gcgcagcgcg ccgagaaact
      841 gatgacgaac ggcggcagcc tcgtcaccct cacctattac ggcgccgaaa aggtcatgcc
      901 gcactacaac gtgatgggcg tcgccaaggc cgcgctggaa gccagcgtgc gctatctcgc
      961 cgaagacctc ggcaagagcg ccatccgggt gaatgccatc tcggcggggc cgatcaagac
     1021 gctggccgcc tccggcatcg gcgatttccg ctacatcctc aaatggaacg aatacaactc
     1081 gcccctgcgc cggacggtga gccaaacgga ggtcggaagc gcggcgttat tcctgttatc
     1141 cgatctcggc aaggcggtga ccggcgaatg cctgcacgtg gacgccggct atcatatcat
     1201 cggcatgaag gcgccggatg cgcccgatat cgtcgtgggc aagaacggag actgaccagc
     1261 tttatggcgc gggccgaaca catgaactgt gttactctca tgcttcgaca ggttctgcct
     1321 gaaggatcag cccaaaccct cgccttgagc ttgtcgaggg gcgagggcgg atagcctctg
     1381 atgtctcaca acattttcgg ccatctgttc cgggtcacga cgttcggcga aagccatggc
     1441 ccggcgctgg gctgcgtgat cgacggctgc ccgccgggca ttccgcttac cgcagccgac
     1501 atccaggccg acctcgaccg ccgccggccc ggccaatcga aattcgttac ccggcgcaag
     1561 gaacccgacc gcgtgcgtat cctctccggc gtcttccagg acgagcggat gaagagccaa
     1621 gtgacgacgg gcacgcccat cgcgctcctg atcgagaatg tggacgcgcg ctcgaaggac
     1681 tattccgaca tccgcgacaa gttccgcccc ggccacgccg actataccta ttgggccaaa
     1741 tacggcgtgc gcgactatcg cggcggcggc cggcagagcg cgcgcgaaac cgccgcgcgg
     1801 gtggcggcgg gagccgtcgc gcgcaaggtt ctggcttcgc tgtaccgggg cgtgcgggtg
     1861 cgcggggcgc tgatccagat ggggacgcag accatcgaac gcaaacgctg gaactggaac
     1921 gaggttccgc gcaatccgtt cttctgcccc gacgtccagg cgatcgctcc gtggacggaa
     1981 tatctggagt gcgtgcgcaa gaagggctcg tcctgcggcg ccatcgtgga agtgacggcg
     2041 gaaggcattc ccgccgggct gggcgcgccg gtctacggca agctcgacgc cgatatcgcc
     2101 tccgcgatga tgagcatcaa cgcggtcaag gcggtggaga tcggcgacgg cttcgccacc
     2161 gccgcgctgt cgggcgcgga caattccgac gacatgcggg ccggacgcgg ccgcaagccg
     2221 aagttcctct ccaatcacgc gggcggaatt ctgggcggga tttccaccgg ccagcccatc
     2281 gtggcgcgct tcgcggtgaa gccgacctcc tccatcctca ccccgcgcaa gaccattgat
     2341 attcacggga acgataccga tatcgtcacc aagggccgcc acgatccctg cgtcggcatt
     2401 cgcgccgtgc ccatcggcga agcgatgctg gcgctggtgc tggctgatca cgcgctcagg
     2461 catcgcggac aaatgggctg acatcatgct ccaaggagga agcgcatcat ggacgacatc
     2521 atcatgatca tcctaatcat actggccgtt attgctttgt ccgccttgat gtcgatcgtc
     2581 ttcaccgtca agcagcaaac ctgcgccatc gtcgaacgct tcggccgcta cgtgcgcacc
     2641 gccaggccgg gcctgaattt caaggttccc ttcatcgaga acatcgtgca gcgcgtctcg
     2701 ctgcgcgtgc agcagttgat catcgaggtc gaaaccaaga ccttggacaa tgtgttcgtg
     2761 aagctgatcg tcgcggtgca gttccgcgtg gtgcaaggca gcgaagcgga ttcctattac
     2821 aagctccagg accatgtgga gcagatcaaa tcctacgtgc tcgacgtggt gcgcgccaag
     2881 gttcccaaga tgaacctcga cgaagtgttc gagaagaagg acgacatcgg cagcgcggtc
     2941 aaaagcgaac tcgacgtttc gatgaagatc tacggcttcg agattcccaa cgccctagtc
     3001 accgacgtca accccgccga caacgtcaag gcggcgatga acgaaatcca gacccagcgc
     3061 cgcctgcagg acgccgccac cgccaagggc gaagccaaca agatcctggt ggtgaagaac
     3121 gccgaagccg aggcggaatc caagcgcctg caaggcgaag gcatcgccaa gcagcgccgc
     3181 gccatcgtgg acggcctgcg cgaatccatc gccgccttcg ccgatcagat cggcagcgtc
     3241 aacgagcatg aagtgctgaa cctcgtcctg atgacccagt atttcgacac gctgaaggag
     3301 atcggcgtct cgtcgggaag caaggtgatc ctgacgccgc atgcgccggg cggcatgacc
     3361 gacatgaccg aacagctccg cgccgcgatc atccaaggca acgaggcggt ggcagggaag
     3421 aagtagcccc cttcgcctcg ctacgctcgg cacttccccc gcatccgcct tcgcccttcg
     3481 ggctacggcg gatgcagggg cagaaaatga tgatcagcgt tcgaacgacg ccgccaattc
     3541 gatgtggttc gaccacagga actggtctat gggctggacg cgcgtgaggc tgtagccgcc
     3601 gtcgatcaga atgcgtgcgt cgcgggcgaa ggtttcggcg tcgcaggaca cgtaagcgac
     3661 gcgcttcagc ttggagcggg ccagcatgcg cacttgctcc aaggcgccgg cgcgcggcgg
     3721 gtcgagtgcg acggcatcga aggccaataa gtcgttctcg ttgagcggac ggcggaacag
     3781 gttgcgcttt tcgacagtga ttggcttgag gcaggaggtg acgcgggcgg cggcagcgag
     3841 cgcctccagc atcggccctt cgatttccac cgcatgcacg cgggcgcgcc cggccagcgg
     3901 cagggcgaag gttccacagc cggaaaacag atcggcgacg gatttggctt tcgcaagtgt
     3961 ttcgaggacg aaggcttgaa gcgccgcctc gccctcgcgc gtgggctgga ggaacgcctc
     4021 ggagggaacg aacaactccg ccttgccgag acgcacggag ggccgcgcca attcgatcag
     4081 cgtttcgccg tgccgcgcca cgcgcgccag cttcagcttg gccgcccagc gcgccagtcc
     4141 ggcgagcgtg gccgcatcgc cggtcttctg ccagcgcagc gaaagatcga cacccgtgtc
     4201 ggtatcggca aggcgcagct cggctccttc accttggcgc agcagcgtgc ccagcatctc
     4261 gcgcagattg gggacgagcg caaccagccg gggcgtcagc accaggcatt cgtgcatgtc
     4321 cacgatgtcg tgagtgcgcg cgccatggaa accgagcgcc acgccgctct cgcctttcaa
     4381 cgccttgaag gtcgcgcgcc gccgcgtggc aggcgggact tccacgattt cttcgaccga
     4441 agcctcgatc ccatgccggg ccagagcgtc ggtcaccaag gcccgcttga aggcgtgata
     4501 atcctccatg ccctgatggg cgcagccgcc gcaggttcca aagtgaagac aaagagtcat
     4561 gcgcgaacaa tacccctccc cgaaatttgc ttcgcaaatt tcgacctccc tcaacccgtc
     4621 ttcgctgaag cttcgccggg taaccacata agaccggcga agccttagcg tagccggcct
     4681 caaggggagg atggattcct cttcgccgcc agcaggaatt cgcgattgcc gtcgccgccc
     4741 gcgatcgggc tttccatcgt tccgatcacc gaccagccaa gctgggcgcc aagccagccg
     4801 gcgatgcctt gcagcgcttc ctcctgcacc gacttgtcct tcacgatgcc gcccttgccg
     4861 actttgccga taccggcttc gaactgaggc ttgatcagcg cgaccagcca agctccttgg
     4921 cgcatccgct tcaacgcggc gggaagcacc agcttgaggc cgatgaagct gacatcgacg
     4981 acgacggcat ccacctgctc cggcacctgg gttggcccca aatcacgcgc gttgacgcct
     5041 tcgagcagga tcacgcgcgg atcggcggcg agcttggcgt gcatctggcc gtggccgaca
     5101 tcgacggcat agaccttgcg cgcgccgcgt tcaagcagga tttcggtgaa gccgccggtg
     5161 gaagcgccga catcgatgca gatgaggtcg tccggcgaca attcgaaacg atccaaagca
     5221 gcagcgagtt ttaaagctcc gcgcgaaaca tagggatggg gcttgcggta ttcgatgacg
     5281 gcgttttcgg ggatggtctg cgacggcttc gacaccggct taccgtccgc gttgacatgg
     5341 cccgcgcgaa tggcggcctg ggcttccgcg cggctggcgg cataaccttt ctcaaccaga
     5401 aaaacatcgg cgcgggtgat catcggaaaa agaccttagg gaacggcgac gtgtaaacaa
     5461 cacccctccc cgaattgctt cgcaattcga ccctctctca aggagagggt ggaagactct
     5521 ttacgcgatc tggctgacgg cggcggcttc ttcttcgcgg cccagcgcgg ccagcgccgc
     5581 cgccgcgata gaattcgcat ccagcccagc cgccgcgtac atccgcgcct ggctgtcctg
     5641 gttctggaag cggtccggca gcaccatcgg gcggatcttc agccccttgt cgagacagcc
     5701 tgcccaggcc aggaactgca tcacatggct gccgaagccg ccggcggcgc cctcctcgac
     5761 ggtcagcagc acttcgtgct cgcgcgccag ccggaggatg agatcgcggt ccagcggctt
     5821 ggcgaaacgg gcgtcggcca ccgtcacgga aagtccgtag gcggacagtc tctcggcggc
     5881 gaccaatgcc tccgccagcc
//
LOCUS       DASWPU010000355         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_27224,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000355 DASWPU010000000
VERSION     DASWPU010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27224"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1512
                     /gene="xylA"
                     /locus_tag="VGC27_07615"
     CDS             172..1512
                     /gene="xylA"
                     /locus_tag="VGC27_07615"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY0282474.1"
                     /translation="MSSPAYVVKPTSYFGGVKPIKFEGLKSDNPLAFRYYNKDQVVLG
                     KRMEDLLRPAVCYWHSFAWNGHDIFGQGTFDRPWNAGVMDQAAAWAKMDAAFDFFSTL
                     GAPFYCFHDVDAMPAASNIKEHVSNLAATIDRLEKKQAETGIKLLWGTANLFSHPRYM
                     GGASTNPDPDVWAFAATQVRHCMDATKRLGGANYVLWGGREGYDTLLNTSLKHELANF
                     GRFLQLVVEHKHKIGFKGTILIEPKPHEPTKHQYDFDTATVYGFLKKNGLENEVKVNI
                     EANHATLAGHSFDHEIAMADALGIFGSIDINRGDPQNGWDTDQFHNDPLDMTLAMYRI
                     LKAGGFTTGGFNFDAKVRRQSIDPADMFYGHVGGLDLLARTLLSAAALIENGAIDKFV
                     EQRYAKWAGDAGQKIHAKGASLESISDAAVAANINPKPQSGRQEYLENLVNRFV"
     gene            1509..>1849
                     /locus_tag="VGC27_07620"
     CDS             1509..>1849
                     /locus_tag="VGC27_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282475.1"
                     /translation="MTGAAHMMFSFRKIGALLILAGVASWAQSVESKAVESKAENSPA
                     RTDASKVINFGDVAVSLAWSSTWQLESASPKGMPGTVEFHAPDRLQMVTMLTPIGGTP
                     SLSADAAMKELV"
BASE COUNT          382 a          576 c          562 g          329 t
ORIGIN      
        1 gcttgcccga ctggcgaaaa cgcgtgagcg ggcggaggat gtctgccgcc caccgccggt
       61 gcgggtcgtg atcgaaccca atacccgcga catccagcaa ctggctccga agcgcgcaat
      121 gttttcaaag atttatcaag accttcgccc aagatttcga ggagtttgat catgtcgagc
      181 ccagcatacg tagtgaaacc cacgtcctat ttcggcggcg tcaaacccat caaattcgaa
      241 ggtttgaagt cggacaatcc gctcgccttc cgttactaca acaaggacca ggtcgtcctc
      301 ggcaagcgca tggaagatct gttgcgcccg gcggtttgct actggcattc gttcgcctgg
      361 aacggccacg acatcttcgg gcaaggcacc ttcgatcgtc cgtggaatgc gggcgtcatg
      421 gaccaggccg cggcgtgggc caagatggat gcggcgttcg acttcttctc gacgctgggc
      481 gcgccgttct attgcttcca cgacgtggac gcgatgccgg cggcttcgaa catcaaggag
      541 cacgtttcca atcttgcggc gaccattgat cgtctggaga agaaacaggc cgagaccggc
      601 atcaagctgc tgtggggtac ggccaacctg ttctcgcatc cgcgctacat gggcggcgct
      661 tcgacgaacc cggatccgga cgtgtgggcg ttcgcggcca cgcaggtgcg ccattgcatg
      721 gatgccacca agcgcctcgg cggtgctaac tacgtgctgt ggggcggccg tgaaggttac
      781 gacacgctgc tcaacaccag cctcaagcac gagctcgcca atttcggccg cttcctgcag
      841 ctggtcgtcg agcacaaaca caagatcggc ttcaagggca cgatcctgat cgagccgaag
      901 ccgcacgagc cgaccaagca ccagtacgac ttcgatacgg cgaccgtgta cggcttcctc
      961 aagaagaatg gtctcgagaa cgaagtgaag gtgaacatcg aagccaacca cgcgacgctc
     1021 gcgggccaca gcttcgacca cgagatcgcc atggcggatg cgctcggcat cttcggctcg
     1081 atcgacatca accgtgggga tccgcagaac ggctgggaca ccgaccagtt ccacaacgat
     1141 ccgctcgaca tgacgctggc gatgtatcgc atcttgaagg cgggcggctt caccaccggc
     1201 ggcttcaact tcgacgcgaa agtccgccgc cagagcatcg acccggccga catgttctac
     1261 ggtcacgtcg gtggcctcga cctgctggcg cgcacgctgc tgtcagccgc ggcgctcatc
     1321 gaaaatggcg ccatcgacaa gttcgtcgag cagcgttacg ccaaatgggc gggtgatgcg
     1381 ggccagaaga ttcacgccaa gggcgcttca ctcgaatcga tttcggatgc cgcggtggcc
     1441 gcgaacatca atccgaagcc gcagtcgggc cgccaggagt atctcgagaa cctggtcaac
     1501 cgcttcgtat gaccggtgcc gcgcacatga tgttctcgtt ccgcaaaatc ggcgcgctgc
     1561 tgattttggc gggcgtggca tcgtgggccc aatccgtcga aagcaaggct gtcgaaagca
     1621 aggccgaaaa ctcgcctgcc aggacagacg cgagcaaggt gatcaacttc ggcgacgtgg
     1681 cggtttcact ggcctggtcg tcgacctggc agctggaaag cgcctcgccc aaaggcatgc
     1741 cgggcaccgt cgagttccac gcgcccgatc gcctgcagat ggtcacgatg ctgacgccca
     1801 tcggcggcac gcccagtctc agcgccgatg cggcgatgaa ggaactggt
//
LOCUS       DASWPU010000356        11948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_76184,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000356 DASWPU010000000
VERSION     DASWPU010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76184"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1056
                     /locus_tag="VGC27_07625"
     CDS             346..1056
                     /locus_tag="VGC27_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEY0282476.1"
                     /translation="MLWEKERDAAGQGPDFRHIDTWIFDLDNTLYRADTDLFAQIEQR
                     MTQFIAQRLDVHPGEAYKLQHMYFRSYGSSLTGLVKRNGIDAEEFLAYVHDIDLSRLS
                     PDPRLRPAIERLPGRRYIFTNGCRHHAARILAQVGLDGVMDDVWDIRTLNFRPKPQPE
                     AYRIIVEAGGFDPATSAMFEDTAFNLEPAYALGMRTVWLRNGSRWSLQAPNVRPALHQ
                     HIHHEIDDLAEFLHAIRV"
     gene            1075..1893
                     /gene="dapD"
                     /locus_tag="VGC27_07630"
     CDS             1075..1893
                     /gene="dapD"
                     /locus_tag="VGC27_07630"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY0282477.1"
                     /translation="MTLAHIIDQAWETREALNGATQGEVREAVEAALDGLDAGRWRVA
                     EKEAGVWRVNQWLKKAVLLSFRLTEMKPISGAPGGAFWWDKVESKFAGWDEARFRAAG
                     FRAVPGAVVRRTAFIAKGVVLMPSFVNLGAYVDEGTMVDSWATIGSCAQIGKNCHISG
                     GAGIGGVLEPMQAGPTIIEDNCFIGARAEVAEGVIVGEGSVLSMGVYLGQSTKIVDRA
                     TGEVIYGRVPPYSVVVSGTMPAGEGKPSLYCAVIVKRVDEKTRSKTSINELLRD"
     gene            1927..3084
                     /gene="dapE"
                     /locus_tag="VGC27_07635"
     CDS             1927..3084
                     /gene="dapE"
                     /locus_tag="VGC27_07635"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF009557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY0282478.1"
                     /translation="MTILDPVALAQALIRCPSITPKDAGALSVLEGALKLLGFSCHRL
                     VFEEPGTEAVDNLYARIGSDAPLFLFAGHTDVVPPGGQWKSDPFGAEIKDGLLAGRGA
                     VDMKSAVAAFAAAAARHLEKGAPCSIALLITGDEEGEAVNGTRKVLEWMKDKGERIDH
                     CIVGEPSSMTAAGDTIKIGRRGSLNVRVTATGVQGHAAYPARARNPIPALAALTTRLA
                     EHKLDEGTAHFEPSTLSFTGMDVGNPATNVIPGEARAQFNIRFNDLHTPETLMGFIES
                     EAKAVAKQSGCEIALEQSLSGIAFLTKPGAFTDLVAKAVESVTGVPPEFSTGGGTSDA
                     RFIQDFCPVAELGLPNATMHQTDECVAVADIHRLTEIYVALLDAYFKNPPQ"
     gene            3081..3893
                     /gene="murI"
                     /locus_tag="VGC27_07640"
     CDS             3081..3893
                     /gene="murI"
                     /locus_tag="VGC27_07640"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY0282479.1"
                     /translation="MIEAPIGIFDSGMGGLTVMRALAKRLPDERFLYLGDTARLPYGT
                     KSAETVKRYALQAAHALMERKVKLLVVACNTASVSLPALQEALAPLPAIGVIEPGARA
                     ALKASPGGPIAVIGTEGTVKGGAYVRAIHAHSQAPVVQQACPLFVPMAEEGLIDGAIA
                     EAVAHRYLDPLMATMPRPRALVLGCTHFPVLKSVIARVAGEDVILVDSAATTAEAVAQ
                     LLTERGLARKEKNGEPHRFLATDAPDRFARVGEIFLGKAIDPGLVELIDLQN"
     gene            complement(3949..4734)
                     /gene="truA"
                     /locus_tag="VGC27_07645"
     CDS             complement(3949..4734)
                     /gene="truA"
                     /locus_tag="VGC27_07645"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY0282480.1"
                     /translation="MVRYRLIIEYDGAPFVGWQRQDNGPSVQGALEEAIFKLCGESVT
                     VTGAGRTDAGVHAFGQVAHFDLEKEFAPDKIRDALNHFLRPAPAAVVEAAIAAPDFHA
                     RFSAKARHYLYRILCRRAPPALDKGRIWHVVHELDAEAMHFAAQSLVGQHDFTTFRSS
                     ECQAKSPLKTLDKLAVRRAADEIEITASARSFLHNQVRSMVGSLKLVGEGKWHPRELG
                     LALAAKDRSRCGPVAPPDGLHLMRVDYDNHASSLDKLGMRGSA"
     gene            complement(4739..5137)
                     /locus_tag="VGC27_07650"
     CDS             complement(4739..5137)
                     /locus_tag="VGC27_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282481.1"
                     /translation="MIRILLFLALIFLVAYLLLSVARRGRRQRTFVAVTGRTVSVRGA
                     RLNASMEWGAVRRIDVARAPTIGVEGFCVVLFGNNDESFAVHDVYDGFRTFQAEMFAL
                     WPQIKEEWTRIYRGPPDISERATLWKRDGG"
     gene            complement(5140..6054)
                     /gene="fmt"
                     /locus_tag="VGC27_07655"
     CDS             complement(5140..6054)
                     /gene="fmt"
                     /locus_tag="VGC27_07655"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY0282482.1"
                     /translation="MSVRLAFMGTPEFSVPLLAELISRHDVAAVYTQPARPKGRGLAE
                     EPSPVARLAAAHGLVVRTPPTLKSAEAQAEFAALGLDAAVVAAYGLILPKPILDAPRL
                     GCFNLHASLLPRWRGAAPIQRAIMAGDENTAVCVMHMDEGLDTGPVLMAESVCIGRQT
                     CGELQSELARLGASLMVRALAALERGGVTERKQDESGATYAKKILKEESRIDWSKSAF
                     EIDCLIRALSPAPGAWTEAKGERLKILYAEPAEGSGAPGEVLDDDLTVACGSGALRPV
                     RLQRAGRGVMSAAELLRGFALPQGSRLA"
     gene            complement(6313..6828)
                     /gene="def"
                     /locus_tag="VGC27_07660"
     CDS             complement(6313..6828)
                     /gene="def"
                     /locus_tag="VGC27_07660"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0282483.1"
                     /translation="MTIRPILTAPDPRLKAVAADVAKVDGDIRRLIDDMLETMYAADG
                     IGLAAVQIGVAKRVIVMDIDQKDGKKKPRVFVNPKILWASEELASFEEGCLSVPDIWE
                     EVERPARIRAEYLDRDGKTQTLEADGLLADCLQHEMDHLNGILFIDHLSRLKRSIALR
                     KLAKAKKKETA"
     gene            6916..7980
                     /gene="rmuC"
                     /locus_tag="VGC27_07665"
     CDS             6916..7980
                     /gene="rmuC"
                     /locus_tag="VGC27_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY0282484.1"
                     /translation="MDTAFIIMAIALVAAAAIVAFALRRPAAQPDSRLDTVIEAQGKL
                     AGQFQQTVAAQAELQRTLAERIDALNKRLGDSLADSATKTTATLTSIGERLSVIDEAQ
                     KNITALSDRVVSLQDILSDKQARGAWGQDRMEDIVRDQLPPDLYQFQARLSNNSAPDC
                     LIRIPGMAAAIVIDAKFPLEAFEALKAATNDAERKLAAAQLRSDVQKHIADIGRKYLI
                     PGEVQSPALMFVPSESIYAELHTTFPEVMQRARRAQVVIVSPHILWLAVNTIRTLMRD
                     AKMREQASEIQKEVALLLKDVKLLETRVGDLRAHFERASKDLGEIEKPMGRVVARAGR
                     IEAVELSEPDAEKPVLPSPP"
     gene            complement(8352..8741)
                     /locus_tag="VGC27_07670"
     CDS             complement(8352..8741)
                     /locus_tag="VGC27_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282485.1"
                     /translation="MKDEIPAVAAQPAAQPAAAAQPVAAAQPAAVLTLEAELAETLKG
                     VTDCFAVTHKIDFNRDEYGHRRAREIENAVTLLKVSAEVGLAIAKIKGEYNHNINVLH
                     GEIWPPPRRAVSKPQTPEEGRPVQNQG"
     gene            complement(8948..9541)
                     /gene="recR"
                     /locus_tag="VGC27_07675"
     CDS             complement(8948..9541)
                     /gene="recR"
                     /locus_tag="VGC27_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY0282486.1"
                     /translation="MSTIGPEIERLIQLLAKLPGLGPRSARRATLELLKKRETLLEPL
                     TSALREAAAAIKTCEVCGNLDTSSPCSLCRDPRRDARLLCVVEEVADLWALERAGVFH
                     GRYHVLGGALSALDGITPERLNVAALLARAKDVDEIIIAVNATVEGQTTAHYLLDALA
                     STGAKVTRLAHGVPVGGELDYLDEGTLSAAFKARRAL"
     gene            complement(9698..11494)
                     /locus_tag="VGC27_07680"
     CDS             complement(9698..11494)
                     /locus_tag="VGC27_07680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF006585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY0282487.1"
                     /translation="MDETQEPTLGLGPEPSGAGARGAAYQVLARKYRPSDFTGLIGQE
                     ALVRTLSNAFAAGRIAHAFMLTGVRGVGKTTTARIIARALNCIGPDGKRSEPTIQPCG
                     VCEPCIAIAESRHVDVQEMDAASRTGIDDVREIVEGVRYAPASARYKVYIIDEVHMLS
                     KQAFNGLLKTLEEPPPHVKFVFATTEIRKVPVTVLSRCMRFDLRRIETGELVAHLENI
                     AKQEGVKIEAPALALIARAAEGSVRDALSLLDQAISHGDPASPDGSAAAGGITSDTIR
                     AMLGLADRGRVLDLFEKVMGGRIAEALAGLNELYDRGADPLAVMQDLLEIVHFLTRVK
                     VAPNAEGYFDGSSGEAKRAADMAAKLSVPSLTRAWSLLLKGLFEVRDAARPVAACEMA
                     LIRLAYAADLPPTDRLVRDLLEGETVQTSQSQPSASAPPPAGGAARTQSVRAGEGAAR
                     SAANPAPQAATENVPTLRTLEDLAALAQAKGAPVLKVHLENDVHLVSLEAGRLEFRPS
                     ARAPKTLASDLAQKLKDWTGTRWVVTLAREGGAPTLAEQKRAAKAARHERVLQEPLVR
                     AVMDRFPGAEIVAVRDVAAEEVAAPMPEDNEE"
     gene            complement(11518..11612)
                     /gene="ffs"
                     /locus_tag="VGC27_07685"
     ncRNA           complement(11518..11612)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGC27_07685"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            11689..>11948
                     /locus_tag="VGC27_07690"
     CDS             11689..>11948
                     /locus_tag="VGC27_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX-like domain-containing protein"
                     /protein_id="HEY0282488.1"
                     /translation="MIQFSGNPLNRLSEKRSDAAWIAEKLHHPTTLILPLWRLLPLVA
                     GTGEAPKAGFLRPGLAESLAGPDAVSVFLGMDDGHAVFALDIS"
BASE COUNT         2103 a         3927 c         3887 g         2031 t
ORIGIN      
        1 gcgaacgttg cgcggcgcgc gggttgacgg aggcgggctt cgccgagacc aacataaaag
       61 gtgcgccggg ctttgtcgtt cacatatccg ccgacggact ggccgcaacg ctctccggca
      121 agaccaagcc gaaataatcc atgatccgga ggatatcgtc gcgcgacaaa ttcgcatgcg
      181 ccgcggcaac gagctcttgc ctcaattcgc gcatgctggg cattccgctc tgtacaagtg
      241 ccgattgtgc cgccatacct tttaaccggg cggtcaaagg atctggccaa caatgccgcc
      301 cttcggcgct tgcgcgcctt tcatgcttaa tttctggacc gtttcatgct gtgggaaaaa
      361 gagcgggatg cggccgggca aggcccggat ttcaggcata tcgacacctg gatcttcgat
      421 ctcgacaaca cgctgtatcg cgccgacacc gatctattcg cacagatcga gcagcgcatg
      481 acccagttca tcgcgcaacg cttggacgtc catcccggcg aagcctacaa gttgcagcac
      541 atgtatttcc gcagctacgg ttcgagcctg accgggctgg tgaagcgcaa cggcatcgac
      601 gccgaagagt tcctcgctta cgtccacgac atcgacctgt cgcgtctgag tcccgacccg
      661 cggctgcgcc ccgccatcga gcgcctgccc ggccgccgct acatcttcac caacggctgc
      721 cgccaccacg ccgcgcgcat tctggcgcag gtcggcctcg acggcgtgat ggacgacgtc
      781 tgggacatcc gcacgctgaa cttcaggcct aagccgcagc ccgaagccta tcggatcatc
      841 gtcgaggccg ggggctttga ccccgccacc tccgcgatgt ttgaagacac cgctttcaac
      901 ctggagccgg cgtatgccct cggtatgcgg acggtgtggc tgcgcaacgg ctcgcgctgg
      961 tcgctgcagg cccccaacgt ccgccccgcg ctgcaccagc acatccacca cgagattgac
     1021 gatctcgccg aattcctgca tgccatcagg gtctgataac cgccagattc cgccatgacg
     1081 cttgcccaca tcatcgatca agcctgggaa acccgcgagg cgctgaacgg cgccacccag
     1141 ggcgaagtgc gtgaagcggt ggaggccgcc ctcgacggtc tcgacgccgg acgctggcgc
     1201 gtcgccgaga aggaagcggg cgtctggcgc gtgaaccaat ggctgaaaaa ggccgtgctc
     1261 ttgtccttcc gcctgaccga gatgaaacca atctccggcg cgcccggcgg cgccttctgg
     1321 tgggacaagg tcgaatccaa attcgccggc tgggacgaag cgcgcttccg cgccgccgga
     1381 ttccgcgccg tgccgggtgc ggtggtgcgc cgcaccgcct tcatcgccaa gggcgtcgtt
     1441 ctgatgccgt ccttcgtcaa tctgggcgct tatgtcgatg agggcaccat ggtggacagc
     1501 tgggccacca tcggctcctg cgcccagatc gggaagaact gccatatttc gggcggcgcg
     1561 ggcatcggcg gcgtgctgga gccgatgcag gccggaccca ccatcatcga agacaattgc
     1621 ttcatcgggg cgcgcgccga agtggccgaa ggcgtgatcg tgggcgaagg ctcggtgctg
     1681 tcgatgggcg tctatctcgg ccagtcgacc aagatcgtgg accgggcaac gggcgaagtg
     1741 atctatggcc gcgtgccgcc ttattccgtc gtcgtctccg gcacgatgcc ggcgggcgaa
     1801 ggcaagccct cgctctattg cgccgtgatc gtcaagcggg tggacgagaa aacgcggtcc
     1861 aagacctcga tcaacgagct gttgcgagat tgaccgcagc ccggccagca gactaatcct
     1921 aggcccatga caatcctcga tccggtggcg ctggcccagg cgctgatccg ctgcccgtcg
     1981 atcacaccga aggacgcagg cgcgctcagc gtgctggaag gcgcgctcaa gctgctcggt
     2041 ttttcctgcc atcgccttgt cttcgaggaa ccgggcacgg aggcggtgga caatctttac
     2101 gcgcggatcg gaagcgacgc gccgctgttt ctcttcgccg ggcacaccga cgtggtgccg
     2161 ccgggcggcc aatggaaaag cgatccgttc ggcgccgaga tcaaggacgg cctgcttgcc
     2221 ggccgcggcg cagtagacat gaagtcggcg gtcgcggcct tcgccgccgc cgccgcgcgg
     2281 catctggaaa aaggcgcgcc ctgctccatc gcgcttctca tcaccggcga cgaggaaggc
     2341 gaagcggtca acggcacgcg caaagtcctc gaatggatga aggacaaggg cgaacgcatc
     2401 gatcattgca tcgtcggcga gccgtcgtcg atgaccgccg ccggcgacac gatcaagatc
     2461 ggccggcgcg gctcgctgaa tgtacgggtg acggccacag gcgtgcaagg ccatgccgcc
     2521 tatcccgcca gggcgcgcaa tccgattccg gcgctggctg cgctgacgac gcgccttgcg
     2581 gagcacaagc tcgacgaggg caccgcccat ttcgaaccgt ccacgctttc cttcaccggc
     2641 atggatgtcg gtaatccagc gaccaatgtc attccgggcg aggcgcgcgc ccaattcaac
     2701 atccgcttca acgatctgca tacgcccgaa acgctgatgg ggttcatcga aagcgaagcc
     2761 aaggccgtcg ccaaacagag cggctgcgag atcgctttgg aacaaagcct cagcggcatc
     2821 gccttcctga ccaagccggg cgccttcacc gacctcgtcg ccaaagcggt cgaaagcgtg
     2881 accggggttc cgccggaatt ttccaccggc ggcggcactt cggacgcgcg cttcatccag
     2941 gacttttgcc cggtggcgga gctaggcctg cccaacgcca ccatgcacca gaccgacgag
     3001 tgcgtggccg tcgccgacat tcaccgcctg accgagatct atgtggcgct gctcgacgcc
     3061 tatttcaaga acccgcctca atgattgaag ctccgatcgg catcttcgat tccggcatgg
     3121 gcggattgac ggtcatgcgg gcgctggcga agcgtctgcc ggacgaacgc tttctctatc
     3181 tgggcgacac ggcgcggctg ccttacggca ccaagagcgc cgagacggtg aagcgctatg
     3241 ccttgcaggc ggcgcacgcc ttgatggaac gcaaggtgaa gctgctggtc gtggcctgca
     3301 acaccgcatc ggtctcgctg cccgccttgc aggaagcgct ggcgcccttg ccggcgatcg
     3361 gcgtgatcga accgggcgca cgcgcggcgc tcaaggcttc gcccggcgga cccatcgccg
     3421 tgatcggcac cgaaggcacg gtcaagggcg gcgcctatgt ccgcgccatt catgcccaca
     3481 gccaggcgcc ggtggtgcag caggcctgtc cgctgttcgt gccgatggcc gaagaaggct
     3541 tgatcgacgg ggcaatcgcc gaagcggtgg cgcaccgcta tctcgatcct ttgatggcga
     3601 ccatgccgcg gccgcgcgcc ctggttctgg gctgcaccca ttttcccgtg ctcaaaagcg
     3661 tgatcgcgcg cgtcgcgggc gaagacgtga ttctggtgga cagcgccgcg accacggctg
     3721 aagccgtggc gcaacttctg accgaacgcg gcctggcgcg caaagaaaag aacggcgagc
     3781 cgcatcgctt cctcgccacc gacgcgccgg accgcttcgc gcgggtgggc gagatctttc
     3841 tcggcaaagc gatcgatccg ggcctggtcg aactgatcga tctacagaat tgagttgctg
     3901 cttccccacc cttcgacaag ctcagggtga ggggatgtag tttttccttc atgctgagcc
     3961 cctcatcccg agcttgtcga gggacgaagc atgattgtcg taatccactc tcatcagatg
     4021 aagcccgtcg ggcggcgcca ccgggccgca gcggctgcga tccttcgccg ccagcgccaa
     4081 gcccagttcg cgcggatgcc atttgccttc gcccaccagc ttgagcgagc ccaccatcga
     4141 gcgcacctgg ttgtgcagga aggagcgtgc ggacgccgtg atttcgattt cgtccgcggc
     4201 gcggcgcacg gcgagcttgt ccagcgtctt gagcggcgac ttggcctggc actccgaaga
     4261 acggaaggtg gtgaaatcgt gctggccgac cagcgattgc gcggcgaaat gcatcgcctc
     4321 ggcatccagc tcatgaacca catgccagat gcggccctta tcgagggcag gcggggcgcg
     4381 gcggcagagg atgcgataaa gataatggcg cgctttggcc gaaaagcggg cgtggaaatc
     4441 gggcgcggcg atggctgcct ccaccaccgc agcgggcgcg ggacgcagaa aatgattcag
     4501 cgcgtcgcgg atcttgtcgg gcgcgaattc cttttcgagg tcgaaatgcg ccacctggcc
     4561 gaaggcatgc acgcccgcat cggtgcgccc ggcgccggtg acggtgacgc tctcgccgca
     4621 aagcttgaaa atcgcttctt ccaatgcgcc ttgcaccgaa gggccgttat cctggcgctg
     4681 ccagccgacg aaaggcgcgc cgtcatattc gatgatgagg cgatagcgaa ccaatgcctc
     4741 agcccccgtc ccgcttccag agcgttgccc gctcgctgat gtcgggcggg cccctataga
     4801 ttctcgtcca ctcctctttg atttgcggcc agagagcgaa catctcggcc tggaaggttc
     4861 gaaatccgtc gtaaacgtca tgcacggcaa aggattcgtc gttgttgccg aacaagacga
     4921 cgcagaatcc ctcgaccccg atggtcggcg cccgcgccac atcgatgcgg cgaacggccc
     4981 cccattccat ggacgcgttc agccgcgccc cgcgtacgct gacggttcgc cccgtgacgg
     5041 cgacgaaggt tctctgccgc cttccccgcc gggcaacaga caacaggagg taggcgacaa
     5101 ggaaaatcag cgcaagaaag agcaggatac ggatcatctc taagccaatc tgctgccttg
     5161 cggcagcgcg aagccgcgca acaattcggc ggcgctcatc accccgcgtc cggcgcgctg
     5221 gaggcggacg gggcgcaggg cgccggagcc gcaggcgacg gtgagatcgt cgtcgagcac
     5281 ttcacccggc gcgccggaac cttccgccgg ttcggcgtac aatatcttca gccgctcgcc
     5341 tttggcctcg gtccaggcgc cgggcgcggg cgacagggcg cggatcagac agtcgatttc
     5401 aaacgcactc ttgctccaat cgatgcggga ttcttccttg aggatcttct tggcgtaagt
     5461 cgcgccggat tcgtcttgct tgcgctcggt aacgccgccg cgctcgagcg ccgccagcgc
     5521 gcggaccatc aaggaagcgc cgagccgcgc cagctcgctc tgcaattcgc cgcaggtttg
     5581 ccggccgatg cacacgcttt cggccatcag caccgggccg gtatccaggc cctcgtccat
     5641 atgcatgaca cagacggcgg tattctcgtc gcccgccatg atggcgcgct ggatcggcgc
     5701 ggccccgcgc cagcgcggca acagcgaagc atgcagattg aaacagccca gccgcggagc
     5761 atcgagaatg ggtttgggca ggatcagccc gtaagcggcc accaccgccg catcgaggcc
     5821 gagcgcggcg aattccgctt gcgcttctgc gctcttcagc gtcggcggcg tgcgcacgac
     5881 aaggccgtgc gcggcggcga gcctggcgac cggcgacggc tcttcggcca ggccccgccc
     5941 cttggggcgc gcgggctggg tgtaaacggc ggcgacgtcg tgacgagaga tcagttcggc
     6001 gagcaaaggc accgagaatt ccggcgtgcc catgaaggcg agccgcacgc tcatcgtacc
     6061 tgcccgctgt tggacgaggc cgtagcgaca gcgtacggcc gagcgcggcg aagcatgagg
     6121 cggagggtca taactcaatc ccacggttgt cattcccggc cgagcggccg tccgccgcgc
     6181 cagcgcacgg acggcatgcg aggggaaggg aacccaggtg gacagaccgg acacaatcca
     6241 accacctggg tccccttccc tcgccgcgcg aaaagcgcgg ctcgccgggg atgacacggt
     6301 ggtgtattgg cctcacgccg tttccttctt cttggccttg gcgagcttgc gcagggcgat
     6361 ggagcgcttc agccgcgaca gatgatcgat gaagagaata ccgttgagat gatccatctc
     6421 gtgctgcaaa cagtcggcca gcagaccgtc ggcttccagc gtttgcgtct tgccgtcgcg
     6481 atcgagatat tcggcgcgga tgcgcgcggg acgctcaacc tcttcccaga tgtcgggcac
     6541 cgacaggcag ccttcctcga aactcgccag ttcctcggag gcccagagga ttttcggatt
     6601 gacgaagacg cgcggcttct tcttgccgtc cttctggtcg atgtccatga cgatgacgcg
     6661 cttggcgacg ccgatctgca ccgccgccag gccgatgccg tcggcggcat acatggtctc
     6721 cagcatatcg tcgatcaggc ggcggatgtc gccgtcaacc ttggcgacat cggccgccac
     6781 cgctttcagg cggggatcgg gcgcggtcag gatggggcga atggtcatgg gtttgagata
     6841 gggcgggcag gcgccggggt caagatttgg ggcgtttgcg cgtaagatgg gcttcgagct
     6901 ggagagattc gtgtcatgga taccgccttc atcattatgg ctattgcctt ggttgccgcc
     6961 gcggcgatcg tcgccttcgc cttgcgccgg ccggcagcac aacccgattc caggctcgac
     7021 accgtgatcg aagcccaggg caagctcgcc gggcagttcc agcagaccgt cgcggcccag
     7081 gccgagctgc agcgcacgct ggcggaacgc atcgacgcgc tgaacaagcg gctgggcgac
     7141 agcctggccg acagcgccac caagaccacc gcgacgctga ccagcatcgg cgaacggctc
     7201 agcgtgatcg acgaagcgca gaagaacatc accgcgctct ccgaccgcgt cgtctcgctg
     7261 caagacatcc tctccgacaa gcaggcgcgc ggcgcctggg gccaagaccg catggaagac
     7321 atcgtacgcg accagctgcc gccggacctc tatcaattcc aggcgcggct ctccaacaac
     7381 agcgcccccg attgcctgat ccgcattccc ggcatggcgg cggccatcgt catcgacgcc
     7441 aagtttccgc tggaagcctt cgaggcgctg aaggcggcaa cgaacgacgc cgagcgcaag
     7501 ctggcggcgg cgcaactgcg cagtgatgta cagaagcata tcgccgacat cgggcggaaa
     7561 tatctgatcc ccggcgaagt gcaatccccg gcgctgatgt tcgtgccttc ggaatcgatc
     7621 tacgccgaat tgcacaccac ctttcccgag gtgatgcagc gggcgcgccg ggcgcaagtg
     7681 gtgatcgtgt cgccgcatat cctgtggctg gcggtgaaca ccatccgcac cctgatgcgc
     7741 gacgccaaga tgcgcgaaca ggcgagcgaa atccagaaag aggtggccct gctcctgaag
     7801 gacgtgaagc tgctggagac gcgcgtcggc gatctgcgcg ctcatttcga gcgggcgagc
     7861 aaggatctgg gcgagatcga aaagccgatg ggccgcgtgg tggcgcgcgc cgggcgcatc
     7921 gaagcggtag agctatccga gcctgacgcc gaaaaaccgg ttctcccttc gcctccatga
     7981 tttcacaccg cgtccaggcc agcgcctggg cgatggcgaa gcgggcggca tggacgcgct
     8041 cgcgcaccgc tttgcgccgg gcggcaaagg ctttcgagta caacccgtgc ttcagccggt
     8101 tgccgttgcc gggctggccg ccgcgtttac gccgggaacc gctactgcca tggcctgcga
     8161 ccgcgggcca tccaggtgat gcttgcatgg tgctgccggt caaaatggga ttgcagcgca
     8221 aatcgtcggc acggagattg tccccaactg ggtggcccgc attcgcgggc catgacaagt
     8281 gggagaacat cggcgcgacg ggcttttcgt cgagcattcg aacctcatga tttcggaggc
     8341 accctccccc tttatccctg attttggacc ggccgccctt cctcaggcgt ttgaggcttg
     8401 gacaccgcgc ggcgcggcgg cggccagatc tcgccgtgca gcacattgat attgtggttg
     8461 tattcgccct tgatcttggc aatggcgagg ccgacttcgg cgctgacctt gagcagcgtc
     8521 accgcgtttt cgatctcgcg ggcgcggcga tggccgtatt cgtcgcgatt gaagtcgatc
     8581 ttgtgggtga ccgcaaagca atcggtcacg cccttcaggg tttcggcaag ctccgcttcc
     8641 agcgtgagca cagccgcggg ctgggcggca gccacgggct gggcggcagc cgcgggttgg
     8701 gcagcgggtt gcgcggcaac tgcgggaatt tcgtctttca tcgctcatat ctccgtcaat
     8761 acaagcccga tacgataagc gcgattccgc gagcttggat aagtttccgg aaaatccgtc
     8821 aagcgcttgc gcgcgcagca agatcggtgc gcgacgatga tggatagaca aatttgcaga
     8881 tcgcaccccc tccgccgcgc caacgcgcgg cacctccccc gcttcgcggg ggcggaaagg
     8941 agcagagcta cagcgcccgg cgcgccttga aggcggccga cagcgtgcct tcgtcgagat
     9001 aatcgagttc gccgcccacc ggcacgccgt gggccaggcg cgtgaccttg gcgcccgtcg
     9061 aggcgagcgc gtcgagcaga taatgcgccg tggtctgacc ctccaccgtg gcgttcaccg
     9121 cgatgatgat ctcgtccacg tccttggcgc gcgcaagcag cgcggcgacg ttgagccgtt
     9181 ccggcgtgat gccgtcgagc gccgacagcg ccccgcccaa aacgtgatag cggccgtgga
     9241 acacgccggc gcgctccagc gcccacagat cggccacctc ttcgaccacg caaagcaggc
     9301 gcgcgtcgcg gcgcggatcg cggcacaagg agcaaggcga gctggtgtcg agattgccgc
     9361 aaacctcgca ggtcttgatc gcggccgccg cttcgcgcag agccgaggtg agcggctcga
     9421 gcaacgtctc gcgctttttc agcagctcca gcgtggcgcg gcgcgccgaa cgcggcccca
     9481 agccgggcag cttggcgagc aactggatca ggcgttcgat ctcggggcct atggtggaca
     9541 catttgctcc ttgtcattcc cggccaagcg accccggact tgatccgggg gagcgtagag
     9601 ccgggaaccc aggtggagag accagacgca atctttccgc ctggatcccc ttccctcgca
     9661 tcgcttcgcg atgctcgccg gggatgacag ccatttctta ttcctcgttg tcttccggca
     9721 tcggcgcggc gacttcttcg gcggcgacat cgcgcaccgc gacgatctcg gcgccgggga
     9781 agcggtccat caccgcgcgc accaaaggct cctgcaagac gcgctcgtgg cgcgcggcct
     9841 tggcggccct tttctgttcg gcgagggtgg gcgcgccgcc ttcgcgcgcc agcgtcacca
     9901 cccagcgggt gccggtccaa tctttcagct tctgggcgag gtcgctcgcc agcgtttttg
     9961 gcgcgcgcgc gctggggcgg aattcgagac ggcccgcctc aaggcttacc agatgcacat
    10021 cgttttcgag atgcaccttc agcaccggcg cgcccttggc ctgggccaag gcggcgaggt
    10081 cttcgagggt gcgaagcgtg ggaacattct cggtggccgc ttgcggcgca gggtttgccg
    10141 cgctgcgcgc ggccccctcc cccgcccgaa cgctttgcgt tcgagcggcc cctcccgcag
    10201 ggggaggggc agatgcggaa ggctggctct gcgatgtctg taccgtttcg ccttccagca
    10261 gatcgcggac cagtctgtcc gtgggcggaa gatcggcggc ataggccagc cggatcagcg
    10321 ccatctcgca ggcggccacc ggacgcgccg catcgcgcac ctcgaacagg cctttcagca
    10381 gcagcgacca ggcccgcgtc agcgacggca cgctgagctt ggcggccatg tccgccgcgc
    10441 gctttgcctc gcccgacgag ccgtcgaaat agccttcggc attgggcgcc accttcacgc
    10501 gggtgaggaa gtggacgatc tccagcaagt cctgcatcac cgcgagcgga tcggcgccgc
    10561 ggtcgtataa ttcgttcaat cccgccagcg cttcggcgat ccgcccgccc atcaccttct
    10621 cgaacagatc gagcacgcgg ccgcggtcgg ccaagcccag catggcgcgg atggtgtcgg
    10681 acgttatccc ccccgccgcg gcggagccgt caggcgaagc cgggtcgccg tgcgaaatgg
    10741 cctgatcgag caaagacagg gcgtcgcgca ccgagccctc cgccgcgcgc gcaatcaagg
    10801 cgagggccgg agcctcgatc ttcacgcctt cttgtttcgc gatgttttcc agatgcgcga
    10861 ccagctcgcc cgtttcgatg cggcgcaaat cgaagcgcat gcagcgcgac agcaccgtca
    10921 ccggcacctt gcggatctcg gtggtggcga agacgaactt cacgtgcggc ggcggttcct
    10981 ccagcgtttt caacaagccg ttgaacgcct gcttggacag catgtgcact tcgtcgatga
    11041 tatagacctt gtagcgcgcg ctggccggag catagcgcac gccttcgacg atctcgcgca
    11101 cgtcgtcgat gccggtgcgc gaggccgcgt ccatttcctg cacgtccaca tggcgggatt
    11161 cggcaatggc gatgcagggc tcgcagacgc cgcaaggttg gatggtcggc tcgctgcgct
    11221 tgccgtcggg gccgatgcag ttgagggcgc gcgcgatgat gcgcgcggtg gtggtcttgc
    11281 ccacgccgcg aacccccgtc agcatgaagg cgtgggcgat ccgcccggcg gcgaaggcgt
    11341 tggacagggt gcgcaccagc gcctcctggc cgatcaggcc ggtgaagtcg gaggggcggt
    11401 atttgcgcgc cagaacctga taggcggcgc cgcgcgcgcc ggcgccggac ggctccggcc
    11461 cgaggcccaa ggttggttcc tgcgtctcgt ccatgctggc gaccggcaaa gggtgaggtg
    11521 ggagactgac agcgacccgg gccaaattcg ttacggctgc tttcttccga acctgaccgg
    11581 gttggcgaca ggcccgtccg ccaccaatct cccgaatgga gtatagcaag gcgggggcct
    11641 gaagtggcaa gaaagagagg gcacaaacct gttggtttcg aggcgcaaat gatccaattt
    11701 tccggcaatc cgctgaaccg attgagcgaa aagcgcagcg acgcggcgtg gattgcggaa
    11761 aaattgcatc atcccacgac gctgatcctg cctctgtggc ggctgctgcc gctggtggcg
    11821 ggcacgggcg aggcgcccaa ggcgggattt ctgcgccccg gcctcgccga aagcctggcc
    11881 gggccggacg cagtcagcgt gttcctgggg atggacgacg ggcacgcggt cttcgctctc
    11941 gacatttc
//
LOCUS       DASWPU010000357        11313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_27235,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000357 DASWPU010000000
VERSION     DASWPU010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27235"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..935
                     /locus_tag="VGC27_07695"
     CDS             339..935
                     /locus_tag="VGC27_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282489.1"
                     /translation="MMRRIGGTLVALCIASAMAAHAQDAADSFTEGPNGAKVHGPSGF
                     VCPQQFGHFARDAVGERNPEAGADFCAYSALDGVYGMVTLTPLRGAYDPKAMLAPDFV
                     VQEGTGGRMVGETTLLLGPKTAPLSVYTRTYETSKLEALHYRILFTSAAVGPWAVQVT
                     IEYADPRDTDKQADFLNAIYAEAMAKIAGVTIPAPASP"
     gene            complement(910..1218)
                     /locus_tag="VGC27_07700"
     CDS             complement(910..1218)
                     /locus_tag="VGC27_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0282490.1"
                     /translation="MRIFAKDREGRETVIEGRDGWTVMEMLRDSGLPITAECGGACAC
                     ATCHVYIEDGWYEKLPPPSEAETDMLDMALAVQPNSRLSCQLICSEDLDGVKVTLAPE
                     "
     gene            complement(1271..2311)
                     /locus_tag="VGC27_07705"
     CDS             complement(1271..2311)
                     /locus_tag="VGC27_07705"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0282491.1"
                     /translation="MSNDFQTPIETDVAIIGAGPAGLFAVFELGLLDLKAHLVDILDR
                     PGGQCTELYPEKPIYDIPGLPIVTGQELTDRLKDQIKPFGPTFHFGEMANALEKLPDG
                     RWKLSTDVGTEIIAPAIVIAAGGGSFMPKRPPVPGIEAYEGTSVHYAVRKIETFRGKN
                     VLIAGGGDSALDWTLNLAPVAKSLTLVHHRDGFRAAQHSVNRMRELVEQKKINFHVAS
                     VKALHGEGGRLSGVTLSGHDKREWHLETDALLPFFGLTMKLGPIADFGLNLHENLIPV
                     DTARFESQTPGIFGIGDIVTYPGKLKLILSGFHEAALMAQAAFHICRPNEKLRFQYTT
                     SSSNLQKKLGVA"
     gene            2684..2965
                     /locus_tag="VGC27_07710"
     CDS             2684..2965
                     /locus_tag="VGC27_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY0282492.1"
                     /translation="MAEVRAEIDRIDRALVKLLSERQRYIERAAEIKSDRAAVRDEVR
                     VEDVIAKILAEARRADLNADIAEAVWRTLMERSIAFEFRAFDAKSQRSR"
     gene            complement(2950..3300)
                     /locus_tag="VGC27_07715"
     CDS             complement(2950..3300)
                     /locus_tag="VGC27_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY0282493.1"
                     /translation="MSGKQCVVDLAHLARYTGGDEAINAEVLRLFDAQTAEIVGRLQT
                     ILDARDVKSWRDSIHALKGASRGVGAFDMAEAAAFAEPIDPGKDRGNASLALAALKTN
                     AEAVQAFIKAYLER"
     gene            3525..4106
                     /locus_tag="VGC27_07720"
     CDS             3525..4106
                     /locus_tag="VGC27_07720"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0282494.1"
                     /translation="MREVEHAYRARGLASMFQVTSATKPEGLSELLLACGYVGVTPSL
                     VCVAGVDSVRARCAKVADAQTLIQANTGFDGLVISGSHSPADGEERLEILSRIALPHL
                     RVTVAVQGTAVACGMGALADGRVGINLMRTHPNYRRRGYARHVLRAIADWSDAQGASE
                     MFLSVEEANTAARALYESASFLRAYAYRYYRKD"
     gene            complement(4103..4798)
                     /locus_tag="VGC27_07725"
     CDS             complement(4103..4798)
                     /locus_tag="VGC27_07725"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEY0282495.1"
                     /translation="MILLLAASATFLGIHLLVSGTRLRDTIVAKIGENPYLGLFALSS
                     LAAIVWLCIAYNHAFASADNRVLYDLGQGFRNFAIPVVAVAFMLAAPGILMPNPTSVG
                     QTTAGIRGVLRITRHPFLWGTAIWAGYHLLATTTLASVIFFATFLVLALLGTRAIDAK
                     CRRKNGAEWQAIAYQTSNLPFAAIIAGRNRFVASEYFDWRFSVAFVEIILFLLFHNYL
                     FSMSPFPNNWLPF"
     gene            complement(4819..5673)
                     /locus_tag="VGC27_07730"
     CDS             complement(4819..5673)
                     /locus_tag="VGC27_07730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0282496.1"
                     /translation="MLEDKASATAQSVALHRAAHQLLDRPAVFSDPLALRIIGREAET
                     ALREGAANHILTGAAGLRAFLTVRSRFAEDCLAEAIDRGVGQYVLLGAGLDTFAYRGT
                     YDPARLRVFEIDHPATQEWKRRRLGDADIAVPGSVHFVPADLERETAAEGLARAGFDR
                     AAPALFALLGVLTYLTQEAAMEILRFAARQPGVSSEIIFDYPEPADAIGPAQRRAMQR
                     LAARVAAVGEPFQTAFKPDEIKEILLALGFSHIEDMDTAALNARYCQGRDDAFALRGS
                     AHIMHAWR"
     gene            6060..7976
                     /locus_tag="VGC27_07735"
     CDS             6060..7976
                     /locus_tag="VGC27_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282497.1"
                     /translation="MAVMVFAITLSAFWAGAAGAYLSGYFGPKGLAALDVQQIALFVA
                     ATFVPPLLFIAAAWALARGQAMGSAAEALVEATDRLFSVDETASRTAARLGRAVRREL
                     DALNAGLDGAFTRLRALESVLENQIASLDEAGARVDVRGEALTARLIQERERIEGVAG
                     TLTDAAARASETVAGRTAQLKAVIETAEGTLKTAGQLLDSQSANFRQAANAAADAPHA
                     AAVELDKQAKRIEAVADSAMARAEFVLGRQERHRTAMNELMLRLKDESVAFENSLSAE
                     REAMEKAIASLGGEAQRFETVTGDAERHLELIMANAAARTSQLTQGFAREADKLKEAS
                     EAANITLVRLVDSLHEAGAGAQTLIGETASQAKADTRALVGEAMAECERLLRTAGELS
                     AEAGEIRTTLAKTVEEVERHLLSLPGIAQQEAQRVRQMVRAETEEILDLSARTLSTVH
                     ARAASKPTSRQKPEQPEEPDADGLRGLARRLTQRSKRKPGEGEPKNWQMSTLLAAADT
                     GDAPKDLKPAAAAALGALQAVLSDMAVDLDAITADAEPHEEEWRRYLAGDRGVFARKL
                     AASIDGEAVNRIATLYRENARFRESATAYMEEFEALLARAREGDGGGLLASTILSADT
                     GKIYLAIAYALGRL"
     gene            7997..8758
                     /locus_tag="VGC27_07740"
     CDS             7997..8758
                     /locus_tag="VGC27_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HEY0282498.1"
                     /translation="MFLMYVDESGDKGLINSPTRYFVLTGLVIHELRWHDALGALVTF
                     RRRMREKFGLKLREEIHSGPMLTRPGSLVRIQKNDRLTIIRHLLDEIASWGAVSLITV
                     RVDKYGKPIGYDPFVKAWEALIQRFENTINHRNFSGPANADDKGIIFCDETDSLVIRQ
                     LYRRMRAHNPVPNQSGLGYRQLPLVRIIEDPVTRNSEHSYFIQAVDAAAFAAYQWYTP
                     SAYVRKKGARNYFRRLEPVLCKVASPRNALGVVEL"
     gene            complement(8949..10241)
                     /locus_tag="VGC27_07745"
     CDS             complement(8949..10241)
                     /locus_tag="VGC27_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY0282499.1"
                     /translation="MIASGHPARAAAQKVLCEVLRQRRPLDAALAAMAPLEARDAGFA
                     RVIASETLRRLGQLEALIRTYVPIPPARNRTGSTPEILLAGACELLFLDVAAHAAVDA
                     ANRLALADDKALHFKSLINAVLRRVAREGKEVVAAQDAARLNAPDWLWQRWLETYGET
                     TTRAIASAHLGVPPLDIVTKGTTPGIPDASALFGQVWRLDEPARVDTMPGYAEGEWWV
                     QDAAATLPAHLLGDVRGKPVLDLCAAPGGKTAQLAVAGAKVTAVEREASRLGRLKENL
                     ERLHLDVNLIVEDVRDFRPEPAPFVLLDAPCSATGTIRRHPELPWIKGAGDVTLCAQA
                     AGELLDAAADMTAEGGLLVFAVCSLEREEGPEQTDEFLRRRPEFSRQAIAPREVFDHG
                     ELLDANGDLRTLPCHLAERGGMDGFYAARFRRTFEPHT"
     gene            complement(10238..11098)
                     /gene="htpX"
                     /locus_tag="VGC27_07750"
     CDS             complement(10238..11098)
                     /gene="htpX"
                     /locus_tag="VGC27_07750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEY0282500.1"
                     /translation="MNALRTGLLMALLTGLFVTVGFLIGGPEGMFLAFLFALGSNLFA
                     YWNSDKIVLAMYRAREVDEQSAPRLVRIVRQLSRAAALPMPKVCIVENPQPNAFATGR
                     NPEHAAVCVTTGLLERVSDEELSGVLAHELGHVRNRDTLTMTIVATIAGAVSMLANFA
                     FLFGGNRRNPLGLIGVLLVSLLAPLAAVLVQATISRAREFEADRAGAEISGRPLWLAS
                     ALAHIDRAARATGNPQADANPATAHMFIVNPLHGGFAGLFSSHPPTEERIARLRAMAG
                     APAKAAGPWG"
BASE COUNT         2177 a         3488 c         3589 g         2059 t
ORIGIN      
        1 agcgcggcgc gcacccgctc gcggctttcg ccgacggcct tgtcggccag tcccaccagg
       61 ttgaactgcc cgccgcccga gtccgcgata tggacctgca cgtccacttc gcgcgcttcg
      121 atgccctgga aggccacggt gtagatatga gcgacggtca tgagtgcggc ggaaccagcc
      181 cggcggccgc gtccgcggtc aatatgccca cgcagtttcc cccctttgcc ccggtaaagg
      241 ctaatctagc gttggaatgg ggcaaggacc atcttgcacg cgcacatacg cacacctagg
      301 cttcattgtt cgtttgtgga atggcgggag aacagcgaat gatgcgcagg atcggtggaa
      361 cccttgtcgc cttgtgtatc gcttcggcga tggccgccca cgcccaggac gcggccgatt
      421 cattcaccga gggcccaaac ggcgcgaaag tgcatggccc gtctggcttc gtctgcccgc
      481 agcagttcgg tcatttcgcc cgcgacgcgg taggcgagcg caatccggaa gcgggggccg
      541 atttctgcgc ctattccgcg ctcgacggtg tttacggaat ggtcacgctg acgcctttgc
      601 gcggggctta cgatcccaag gcgatgctgg cgccagattt cgtggtgcag gaaggcaccg
      661 gcggcaggat ggtcggcgag acaacgctgc tcctggggcc gaagaccgcg ccgctatccg
      721 tctatacgcg cacctacgaa acctcgaaac tcgaagcgct gcactaccgg atcctgttca
      781 ccagcgccgc tgtggggcca tgggccgtgc aggtcaccat cgagtatgcc gacccgcgcg
      841 acaccgacaa acaggccgat ttcctgaatg cgatctatgc cgaagcgatg gccaagatcg
      901 ccggggtcac tattccggcg ccagcgtcac cttgacgccg tcgaggtctt ccgagcagat
      961 gagttggcag gacaaccgcg aattcggctg cacggcgagc gccatgtcca gcatgtcggt
     1021 ttccgcctcg ctgggcgggg gcagcttctc gtaccagccg tcctcgatat agacgtggca
     1081 agtggcgcag gcacaggccc cgccgcattc cgccgtgatc ggcaggccgc tgtcgcgcag
     1141 catctccatg accgtccagc cgtcgcggcc ctcgatgacg gtttccctgc cttcgcggtc
     1201 tttcgcaaaa atcctcatca atgacgctcc tcaccctgag gggcctgcca agggcgggcg
     1261 tctcgaaggg tcatgccaca ccgagtttct tctgcaagtt cgacgacgag gtcgtgtatt
     1321 ggaagcgcag cttttcgttc ggccggcaga tgtgaaatgc cgcctgggcc atcagcgccg
     1381 cttcatgaaa gccggacaaa atcagcttca gcttgcccgg ataggtgacg atgtcgccga
     1441 tgccgaaaat gcccggcgtc tggctttcga aacgcgccgt atccacggga atcagatttt
     1501 cgtgcaggtt gaggccgaaa tccgcgatcg gtcccagctt catcgtcagc ccgaagaaag
     1561 gaagcagcgc atcggtttcc agatgccatt cgcgcttgtc gtggcccgat agcgtcactc
     1621 cggaaagccg tccgccctcg ccgtgcagcg ccttgacgct ggcgacgtgg aaattgattt
     1681 tcttctgttc gaccagttcg cgcatccggt tgacgctgtg ctgggcggcg cggaagccgt
     1741 cgcggtgatg caccagcgtc aggctcttgg cgaccggcgc caggttcagc gtccagtcga
     1801 gtgcggaatc gccgccgccg gcgatcagca cgttcttgcc gcggaaggtt tcgatcttgc
     1861 gcacggcgta gtggaccgag gttccttcat aggcttcgat accgggaacg ggcgggcgct
     1921 tgggcataaa gctgccgccg ccggccgcga tcacgatcgc gggagcgatg atctcggttc
     1981 caacgtcggt cgagagtttc cagcgtccgt cgggcagttt ttccagggcg ttcgccatct
     2041 ccccgaaatg gaaggtgggg ccgaacggct tgatctgatc cttcagccga tcggtcaatt
     2101 cctggccggt gacgatgggc agccctggga tgtcgtagat cggcttttcg gggtagagct
     2161 cggtgcattg cccgccgggc cggtcgagaa tgtcgacgag atgggccttg aggtccaaca
     2221 gaccaagctc gaacacggca aaaaggccgg ccggccccgc gcctatgatc gcgacatcgg
     2281 tttcaatagg agtttggaag tcgttactca ttgatatttt acactctaga attgtacaag
     2341 aacggaaatt tgcgttctta tatgtaaaaa gcccggcacc aacaatcccc tccctctata
     2401 gaaccgttca ctagtgtcgt ggtttcgaag ttcgcagcat gcgcactatc gggctgcaag
     2461 cgaacttcta aaccaaaacg acactaggat tcaataggtt gctagcgtcc cttcgattcc
     2521 gaaattcgcc cgaagccgat atagagaggg tgcgaatttc ggaagcggta cactagcgtc
     2581 agaacattgt ttttgctcat attccacaga tatctggcag acgggacaat tggccatgac
     2641 gaccgcggat tcgcaagacc gggagccagg gcgatgcgag aacatggccg aggtgcgggc
     2701 cgaaatcgac cggatcgaca gagcgctggt gaagctcctt tccgagcgcc agcgctatat
     2761 cgaacgcgcc gcggaaatca aatccgatcg cgccgccgtc cgcgatgaag tacgggtcga
     2821 agacgtgatc gccaagatat tggccgaagc ccgcagggcg gacctgaacg ccgatatcgc
     2881 cgaagcggta tggcgaacgc tgatggaacg ctcgatcgcc ttcgaatttc gcgcgttcga
     2941 cgcgaaaagt cagcgctcca gataggcctt gatgaaggct tgaaccgctt cggcgttggt
     3001 tttcagagcc gccaaggcca gcgaagcgtt gccgcggtcc ttgccggggt cgatcggttc
     3061 ggcgaacgcc gccgcctcgg ccatgtcgaa tgccccgacg ccgcgcgacg cgcctttgag
     3121 cgcatggatg gaatctcgcc aggatttgac gtcgcgggca tcgagaatgg tttgcaggcg
     3181 cccgacgatt tcagctgttt gcgcgtcgaa aagcctcaga acctcggcat ttatggcctc
     3241 gtcgcccccc gtataacggg caaggtgggc gagatcgacg acacattgtt tgcccgacat
     3301 gggcggatcc ccgttgtggc acatggagga tggagtaatt gcgtcaagag cgagttaacg
     3361 aggcagattg ggcccgacgc cttgagccgc cgtcgctttt aacctggccg gcccagcaga
     3421 cggcacaggt gtcgggatgg ttgttgcgat acacgagcgg gctaagccat cgcgccaatt
     3481 cggtcgccac actcgaattt cacggccggg atgtgacgag tgccatgagg gaagtcgagc
     3541 acgcctatcg tgcccgcggt cttgcctcca tgtttcaggt gacatccgct accaagccgg
     3601 aaggcctctc ggaacttttg ctggcctgcg gatatgttgg cgttacgcct tcgctggtct
     3661 gcgtcgccgg cgtcgattct gtacgcgcgc gatgcgcgaa ggtcgccgat gcgcagacgc
     3721 tgatccaggc caacacaggc ttcgacgggc tggtgatctc gggttcgcat tcaccggcgg
     3781 atggcgagga gcgcctcgag atcctgtcgc gcatcgcgct gccgcatctg cgtgtcacag
     3841 tcgccgtgca gggtaccgcc gtcgcctgcg gcatgggcgc gctggctgac ggccgcgtcg
     3901 gtatcaatct catgcggacg catccgaatt accggcggcg cggctacgcg agacacgtgc
     3961 tgcgtgcgat tgccgattgg agcgacgccc aaggcgcaag cgagatgttt ctcagcgtgg
     4021 aggaagcaaa cacggcggcg cgcgcgctgt atgaaagcgc cagcttcctg cgcgcctatg
     4081 cttatcgcta ttaccgcaag gactagaacg gcagccaatt atttggaaac ggcgacatgc
     4141 tgaacagata attgtggaac agcaggaaca ggatgatttc gacgaacgcc acgctgaagc
     4201 gccagtcgaa atattcgctc gcgacgaaac gattgcgccc ggcgatgatc gcggcgaagg
     4261 gaaggttcga ggtctgatag gcgatggctt gccactcggc gccgttcttt cttctgcatt
     4321 tggcgtcgat ggcgcgggtg ccgagcagcg ccagaaccag gaaggtggcg aagaagatca
     4381 ccgacgccag cgtggtcgtg gccagcagat gatatcccgc ccagatcgcc gtgccccaca
     4441 gaaagggatg acgggtaatg cgaagcacgc cgcgaatgcc ggcggttgtc tgtcccaccg
     4501 aggtcggatt gggcatcaga atgccgggcg ccgccaacat gaacgcgacc gccacaacgg
     4561 ggatcgcaaa attgcggaag ccttggccca gatcgtagag cacgcgattg tccgcgctgg
     4621 cgaaggcatg gttgtaggcg atgcagagcc agactatggc cgcgagcgag gatagcgcga
     4681 acagcccgag ataaggattt tcgccgatct tcgccacgat ggtgtcgcgc agcctggttc
     4741 cggaaaccag cagatggatg ccgagaaatg ttgcgctggc ggctagtaac aggatcatga
     4801 cgcaccccct tcgcgttcct atctccaggc gtgcatgata tgcgcgctgc cgcgcaaggc
     4861 gaaggcgtca tcgcgtcctt ggcaatagcg ggcgttcagc gccgccgtat ccatgtcttc
     4921 gatgtggctg aagccgaggg ccaggagaat ctccttgatc tcgtccggct tgaaagcggt
     4981 ttggaacggc tcgccgacgg cagccaccct cgcggcaagc ctttgcatgg cccggcgctg
     5041 tgccgggcct attgcgtcgg cgggttcggg ataatcgaat atgatttcgc tggatacgcc
     5101 gggttgccgg gccgcgaagc gcaggatctc cattgcggct tcctgcgtca gatatgtcag
     5161 gacgcccagc aaagcgaaga gtgcaggcgc cgcgcggtcg aacccggccc gcgccagacc
     5221 ttctgccgcg gtctcgcgct cgagatccgc cggcacaaag tgaaccgaac ccggcacggc
     5281 gatatcggcg tccccgaggc gccggcgttt ccattcctgg gtagccggat gatcgatctc
     5341 gaagacgcgc agacgcgccg gatcgtatgt accccgatag gcaaaggtat ccagtcccgc
     5401 accaagaagc acatactggc cgaccccgcg atctatcgct tcggcgagac agtcctccgc
     5461 aaagcggctg cggacggtca ggaacgcccg caatcccgcc gcacccgtca ggatgtggtt
     5521 ggccgcgccc tcgcgcaacg ccgtttcggc ttcccggccg atgatccgaa gggcgagcgg
     5581 atcggaaaag accgcagggc ggtccagcaa ctgatgcgcc gcccggtgaa gggcgacgct
     5641 ttgggccgtg gcgctggctt tgtcctcaag cactctttgt caatccccgt ttttcatggc
     5701 gatctatagc cgaattggct ttggtcctcg ggctttggat ggttaaacta gtgtcgtggt
     5761 ttcgaagttc gcactatccg cactatcggg ctgcaagcga acttcgaaac caaaacgata
     5821 ctaggattca atacgttgct cgtgtccttt cgattccgaa attcgcccga agccgatata
     5881 gagatggggc gaatttcgga atcgggacac tagcagccag tccgcgcaag ttgggggtgt
     5941 ttatcgtgag cagcgtatcc gaccgtaacc agccggtcct gttgcagtct gccgactccc
     6001 tggaggggga aatggagact cgctcgcgcc ggaaatcagg ctttgattcg aagttcggcg
     6061 tggccgtcat ggtcttcgcg atcaccctgt cggcgttctg ggcgggcgcg gccggagcct
     6121 atctgtcggg ctatttcggg cccaaggggc tggccgctct cgatgtccag caaatcgccc
     6181 tgttcgtggc ggcgaccttc gtgccgccgc ttctgttcat tgccgccgcc tgggcgctcg
     6241 cccgcggcca ggcgatggga agcgccgccg aagccttggt ggaggcgacg gaccggcttt
     6301 tctccgtcga tgagacagcc tcgcgcacgg ccgcccgtct tgggcgcgcc gtccggcgcg
     6361 aactcgacgc gctcaatgcc ggtctcgacg gcgcgttcac gcggcttcgt gcgcttgaaa
     6421 gcgtgctgga gaaccagatc gcttctctcg acgaggccgg cgcgcgcgtc gacgtgcgcg
     6481 gcgaagcgct gacggcgcgc ctcatccagg aacgcgaacg gatcgaaggc gtggcgggca
     6541 cgctgacgga cgccgccgcg cgcgccagcg aaaccgtggc gggccgcacc gcccagctca
     6601 aagcggtcat cgagacggcc gaaggcacac tcaagacggc ggggcagttg ctcgacagcc
     6661 aatcggcgaa tttccgtcag gcggcgaatg ccgcggccga cgcgccgcac gccgccgccg
     6721 tcgaactcga caagcaggcc aagcgcatcg aggccgtcgc cgattctgcc atggcgcgcg
     6781 ccgaattcgt gctcggccgg caggagcgcc atcgcaccgc catgaacgag ctgatgttgc
     6841 ggttgaagga cgaaagcgtc gctttcgaga actcgctgtc cgccgaacgt gaagcgatgg
     6901 aaaaagcgat cgcatccctc ggcggcgagg cgcagcgctt tgagacggtc acgggcgatg
     6961 ccgaacgcca tctcgaactc atcatggcca atgccgcggc gcggaccagc cagctcaccc
     7021 aaggattcgc gcgcgaagcc gacaagctca aggaggcgag cgaagcagcc aacatcacgc
     7081 tcgttcgcct ggtcgactcc ttgcacgaag cgggcgcggg ggcgcagacc ctgatcgggg
     7141 aaaccgcaag ccaggcgaaa gccgatacgc gggccctggt cggcgaggcg atggccgaat
     7201 gcgaaaggct gttgcgcacg gcgggcgagc tttccgccga agccggcgag attcgcacca
     7261 cccttgccaa aaccgtggaa gaggtggagc gccatctgct ctctctgccc ggcatcgccc
     7321 agcaggaagc ccagcgcgtg cgtcagatgg tgcgggccga aaccgaagag attctcgatc
     7381 tttcggcgcg gacgctttct accgttcacg cgcgcgctgc ttccaagccc acctcgcggc
     7441 aaaagccgga gcaacccgag gagcccgatg ccgacggctt gcgcggtctg gcccgcaggc
     7501 tgacgcagcg ttccaagcgc aagcctggag aaggcgaacc caagaattgg caaatgagca
     7561 ctttgctggc cgccgccgac accggcgacg cgcccaagga tttgaagccg gctgccgcgg
     7621 cggcgctagg cgcgctgcaa gccgtcctct ctgacatggc cgtggacctg gacgcgatta
     7681 cggcggatgc cgagccgcat gaagaggagt ggcggcgcta cctggcgggc gatcgcggcg
     7741 tcttcgcccg caagctggcg gcgtcgatcg acggcgaagc ggtcaaccgc atagccacgc
     7801 tctatcgcga gaacgcgcgt ttccgcgagt ccgccaccgc ctatatggaa gagttcgagg
     7861 cgctgctcgc ccgcgcccgc gaaggcgacg gcggcggact gctcgcctcc accatcctct
     7921 ccgccgacac cgggaagatc tatctcgcca tcgcctatgc cttagggcgg ctataaagac
     7981 aaagtgtgtg gcttcgatgt tccttatgta tgtggatgag agtggcgata aaggcctgat
     8041 caattcgccc acccgctatt ttgttttgac agggcttgtt atccacgaac ttcgatggca
     8101 cgacgcgctt ggggcgctcg tgaccttccg tcgacgtatg cgcgaaaagt tcgggctaaa
     8161 attacgggag gaaattcaca gcggcccaat gctcactcga cccggtagtc tggtaagaat
     8221 tcagaagaac gatcgcctaa cgattattcg tcacttgctg gacgaaattg cctcatgggg
     8281 ggcagtcagt cttatcactg tgcgtgtcga taagtacggc aaaccaattg gctatgatcc
     8341 atttgtgaag gcctgggagg cgttgattca gcgcttcgag aatacgatca atcaccgtaa
     8401 tttttccggc cctgcaaacg cagacgacaa gggaattata ttctgcgatg agacagacag
     8461 cctcgtaatc cggcaactct atcgcaggat gagggcccac aatccggttc caaaccaaag
     8521 tggcttgggc tatcggcaat taccgctcgt gcgcattatt gaagacccag tcacacgcaa
     8581 ttctgagcat tcatatttta ttcaagctgt ggatgcggct gcattcgcgg catatcaatg
     8641 gtatacgcct tccgcttacg tgaggaagaa gggtgcgcgg aattacttta ggcgcttaga
     8701 accggtgttg tgcaaagttg catccccacg taacgcactt ggtgttgttg aactataaaa
     8761 taaaggggag gcctttcgac ctcccccggt gaaatcctac taaccagtct agccggggtt
     8821 cagatttcca cccaacgata tgattcagtt gggtaaaatg caactctgct gatttaacga
     8881 atctcatcag tgggttgcgg ggtgtgccac aacagctatc tataaatagc taacattcgt
     8941 gagtgggttt acgtgtgtgg ctcaaatgtg cgtctgaagc gggcggcgta gaacccgtcc
     9001 atgccccctc tttccgccaa atggcagggc agggttctaa ggtcgccgtt ggcgtcgagc
     9061 agttcgccat gatcgaagac ttcgcgcggc gcaatggcct ggcgcgagaa ttccggccgg
     9121 cggcggagga attcgtccgt ttgttcgggc ccttcttcgc gttcgagcga acacaccgca
     9181 aagacgagca acccgccctc ggccgtcatg tcggcggcgg cgtccagaag ttctccggcc
     9241 gcttgggcgc agagcgtgac gtcgccggcc cctttgatcc agggcagttc gggatgccgc
     9301 cggatcgttc cggtggcgct gcatggcgca tccagcaata cgaacggcgc gggttcgggc
     9361 cggaaatcgc gcacgtcctc gacgataagg ttcacatcga ggtgcaagcg ctcaagattc
     9421 tccttcagcc gtcccagacg cgatgcttct cgctcgaccg ccgttacctt tgctccggct
     9481 accgccagtt gcgccgtctt gccgcccggc gcggcgcaca gatcgagcac cggttttccg
     9541 cgcacatcgc cgagaagatg ggccggcagc gtcgcggccg cgtcctggac ccaccactcg
     9601 ccttcggcgt agccgggcat tgtatcgacg cgcgcgggct cgtcgaggcg ccagacttgg
     9661 ccgaacagcg ccgatgcatc gggaatgccg ggcgtggtgc ccttcgtcac gatgtcgagc
     9721 ggcggcacgc ccagatgcgc cgacgcgatc gcccgcgtcg ttgtctcgcc ataggtctcc
     9781 agccagcgct gccaaagcca gtcgggcgcg ttgagccggg cggcgtcttg cgcggcgaca
     9841 acctctttgc cttcgcgggc gacacgccgc agcaccgcat tgatcagaga cttgaaatgc
     9901 aaggccttgt cgtcggccag ggccaggcgg ttggcggcgt ccaccgcggc atgcgccgcc
     9961 acgtcgagaa acaagagttc gcaagcgccc gccagcagga tttccggcgt cgaaccggtg
    10021 cgattgcgcg ccggtggtat tggcacataa gtccggatca aagcctcgag ctggccgagc
    10081 cgccgcagcg tttcactggc gatgacgcgc gcaaagccgg catcgcgcgc ttcaagcggc
    10141 gccatcgcag ccagcgccgc gtcgagcggg cggcgctggc gcaagacttc gcagagaacc
    10201 ttttgcgccg cggcccgtgc cgggtgtccg gaagcgatca tccccaagga ccggccgctt
    10261 tggcaggcgc tcccgccatc gccctgaggc gcgcgatgcg ttcctcggtt ggcggatggc
    10321 tcgagaacaa gccggcgaag ccgccatgca agggattgac gatgaacata tgcgcggtcg
    10381 cgggattggc gtcggcctgg ggattgccgg tcgcccgcgc cgcgcggtcg atatgcgcca
    10441 aagcggaggc cagccagagc ggccggccgg aaatctcggc gccggcgcgg tcggcctcga
    10501 actcgcgcgc ccggctgatg gtcgcctgca ccagaacggc ggcgaggggc gccagcagcg
    10561 acaccagcag cacgccgatc aggcccaacg gattgcgtct gttgccgccg aagagaaaag
    10621 cgaaattcgc cagcatggaa acggcgccgg caatggtcgc gacgatcgtc atggtcaggg
    10681 tatcgcggtt tctcacatgg ccgagttcgt gcgccaggac acccgacagc tcctcgtcgc
    10741 tgacgcgctc gagcaggcct gtcgtcacgc agaccgccgc gtgttccgga ttgcgcccag
    10801 tggcgaaggc attgggctgc gggttctcga cgatacagac cttcggcatc ggcagcgcgg
    10861 cggcccggct caattggcgt acgatccgaa ccagcctcgg cgcggattgc tcgtcgacct
    10921 cgcgcgcgcg gtacatggca aggacgatct tgtcggagtt ccagtaggcg aagagattcg
    10981 atcccagagc gaacaggaac gccaaaaaca tgccctctgg cccgccgatc aggaagccga
    11041 ccgtgacgaa aagtcccgtc agcaaggcca tcaaaagtcc ggttcgcagc gcgttcaaag
    11101 tctcttctcc gcctgtacca agggtgatgt atggtccgct gcgggcgcgt caagcggcag
    11161 gcaccatggc aaaacgaaaa acggcaaaat cgccgccaaa gaccccggcc aggctggtca
    11221 aggcccgcat cgccgaagcc gccaaacgcg ccctcgccga agccgaggcc cgccgccaag
    11281 ccccgccggc gaaccgcccc gccgaacatg gcg
//
LOCUS       DASWPU010000358         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_64539,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000358 DASWPU010000000
VERSION     DASWPU010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64539"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGC27_07755"
     CDS             <1..240
                     /locus_tag="VGC27_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0282501.1"
                     /translation="VRGGLLQVARIAGLTTEKVDQCIANKDEQDRINQVSQDGQLKYG
                     LNAVPAFVINGTLWEAGGVDWPKLKEKLDSLLSKH"
     gene            318..>2697
                     /locus_tag="VGC27_07760"
     CDS             318..>2697
                     /locus_tag="VGC27_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0282502.1"
                     /translation="MEPTELIIEPGLTAIVGPNGCGKSNLFDALRWVMGEARPTSVRG
                     SEMDDVIFAGSAARPARNMAEVTLAIDNSDRTANPPYDHFETIEVSRRIEREAGSVYR
                     INGRDVRQRDVQIFFADASSGASSTAFVRQGQISQLISQKPLARRAILEEAAGITGLH
                     QRRHEAELRLRAAEANLSRLDDVIREVEGQLQSLKRQARQASRYRNLSGHIRRAEALA
                     HYLRWTAAQLRAKAAAEALAAAAAVVATCTEQAAQASTVQAGEAAALPPLRQSEAEKA
                     AALHRLVLEREALEAAETRAREQAQSLRQRLAQTAQDIERERTLQGDAETALAALAAE
                     ANGLEAAQERAEQDIAEAQTRAVELNEALAEAERLLERLTSELAERNARQGSLERSRK
                     VAAELADTSGQQLGEAEARLAAAMEGAAGAPDVRAAEAAAEEARALAQTAAAAAAEAR
                     AALAEADLAERQARAPLEDAEREAQRLAAEAKALSELLRPEGQSLWPPLIDAVTVHPG
                     YEAALAAALGDDLHAPLDEAAPHHWRDLGEFDETAPLPEGAKPLAEFVTGPGALSRRL
                     AMTGLVFPDQGSALQKELKAGQRLVSPRGDLWRWDGYRASADAPSAAAVRLSQRNRLA
                     ALEDEIVQAKETSAAAFAEYSAAKTAAEAAREAAREAEQTERRHEHAIITAQDEATRA
                     VRAAAERASHLASLEAEIRRLTQSRDAAREAEQQAARSLAELGDGVELVHNAANARER
                     AGEARAASANARAALDGLKRDGETRSRRLAVIAEERRRWEDRRSAAAEHIAEL"
BASE COUNT          488 a          947 c          906 g          356 t
ORIGIN      
        1 gtgcgcggcg gcctgcttca agtggcgcgc atcgcggggc taacgaccga gaaagtcgac
       61 cagtgcattg cgaataagga cgagcaggac cgcatcaatc aagtctccca ggacggccag
      121 ctgaaatacg gccttaatgc cgtccccgcg ttcgtcatca acggaacgct gtgggaagcc
      181 ggcggtgtgg actggccgaa attgaaagag aaactggatt ccctgctgtc caaacactga
      241 cggacagcgc agcagcgtag gacggcgcgt atccgttgaa gtttaacaga ttaaggcttt
      301 ccggttttaa gtcgttcgtg gaaccgaccg aactcattat cgagcccggg ctgacggcga
      361 tcgtcggccc gaacggctgc ggcaaatcca acctgttcga tgctttgcgc tgggtgatgg
      421 gcgaagcacg gccgacctcg gtgcgcggct ccgagatgga cgacgtgatc ttcgccggct
      481 cggcggcgcg gcccgcccgc aacatggccg aggtgactct ggccatcgac aattccgacc
      541 gcaccgccaa cccgccttac gatcacttcg agaccatcga ggtttctcgg cgcatcgaac
      601 gcgaggccgg atcggtctat cgcatcaatg ggcgcgacgt gcgccagcgc gacgtgcaga
      661 ttttcttcgc cgatgcgtcc tccggcgcgt cgtccaccgc cttcgtccgc caaggtcaga
      721 tcagccagct catcagccag aagccgctgg cgcgccgcgc catcctggaa gaagcggcgg
      781 gcatcaccgg attgcaccag cgccgtcacg aagccgaact gcgcctgcgt gcggccgaag
      841 ccaatctgtc gcgtctcgac gacgtgatcc gcgaagtgga aggccaattg cagagcctga
      901 agcgccaggc ccgtcaggct tcgcgctatc gcaatctctc cggccatatt cggcgcgccg
      961 aagcgctggc gcactatctg cgctggaccg ccgcgcagct ccgcgccaaa gcggccgccg
     1021 aggcgcttgc cgccgccgct gccgtcgttg cgacctgcac ggaacaggcg gcgcaagcct
     1081 ccacggtaca ggccggtgaa gccgccgctc tgccgccttt gcgtcagtcg gaggcggaga
     1141 aggccgccgc cctgcaccgg ctggtgctgg agcgcgaagc cctggaggcg gcggaaaccc
     1201 gcgcccgcga acaggcccag tcgctgcgcc agcgcctcgc ccagaccgcc caggacatcg
     1261 aacgcgaacg cacgcttcaa ggcgatgccg agacggcttt ggccgcgctc gccgccgagg
     1321 cgaacggcct cgaagccgcg caggaacgtg ccgaacagga catcgccgaa gcacaaaccc
     1381 gcgccgtgga gttgaacgag gcgctggccg aagccgagcg cctgctcgaa cggctgacct
     1441 ccgaactggc cgagcgcaac gcccgtcaag gcagcctgga acgttcgcgc aaggtcgccg
     1501 ccgaactggc cgataccagc ggccagcagc tcggcgaggc cgaagcgcgc cttgccgccg
     1561 ccatggaagg cgcagccggt gcgcccgacg tacgcgccgc cgaagccgcc gccgaagagg
     1621 cccgcgccct ggcgcagaca gccgccgcgg cggctgccga agcgcgcgcc gcgcttgccg
     1681 aagccgacct cgccgaacgg caggcccgcg cgccgctcga agatgccgag cgcgaggcgc
     1741 agcgccttgc cgccgaagcc aaggccctga gcgaattgct gcgccccgaa ggacaaagcc
     1801 tgtggccgcc gctgatcgat gccgtcaccg tgcatcccgg ctacgaagcc gctcttgcgg
     1861 ccgccttggg cgacgattta cacgccccgc tcgacgaagc cgcaccgcat cactggcgcg
     1921 atttgggcga gttcgatgaa accgcgccct tgcccgaagg cgccaagccg cttgccgaat
     1981 tcgtcaccgg cccgggcgcg ctgtcgcggc gtctggcgat gaccggcttg gtcttccccg
     2041 atcagggttc ggcgttgcag aaagaattga aagccggcca gcgtctggtg tcgccgcgcg
     2101 gcgatctgtg gcgctgggac ggctatcgcg cttccgccga cgcgccgtcc gccgcggcgg
     2161 tgcgcctatc gcaaagaaac cggctggcgg cgctggaaga cgagatcgtc caggccaagg
     2221 aaaccagcgc cgcggcattc gccgaatatt cggcggccaa aacggcggcc gaggcggccc
     2281 gcgaagccgc ccgcgaggcg gagcaaacgg agcgccggca cgagcacgcc atcattactg
     2341 cccaggatga agccaccagg gccgttcgtg cggcggcgga gcgcgcctcg cacctggcct
     2401 cgctcgaagc cgaaatccgc cggctgacgc aatcgcgcga cgccgcgcgc gaagccgagc
     2461 aacaggccgc ccggtcgctg gccgaactcg gcgacggtgt ggaactggtc cacaacgctg
     2521 ccaatgcgcg cgagcgggcg ggcgaagccc gcgccgccag cgccaacgcc cgcgccgccc
     2581 tcgatgggct gaagcgtgat ggcgaaacgc gcagccggcg cttggcggta atcgccgagg
     2641 agcgcaggcg ttgggaagac cgccgcagcg ccgcggccga acatattgcc gagctgg
//
LOCUS       DASWPU010000359         3889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_113371,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000359 DASWPU010000000
VERSION     DASWPU010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113371"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VGC27_07765"
     CDS             <1..521
                     /locus_tag="VGC27_07765"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY0282503.1"
                     /translation="YDPALLKERIGPSGQKRSIADRILMPLLGVVYCAWLVFMALDVR
                     WHGFSQMPEWLNYAAGAAILASFAGMVRVFRENSFASSVVRVQKDRGQTVITTGPYAI
                     VRHPMYSIASFTYLVIPFALGSWMGLLAVPLLILGLAIRALVEERVLKEQLESYGDYM
                     AKVRYRFMPYVW"
     gene            complement(509..1087)
                     /locus_tag="VGC27_07770"
     CDS             complement(509..1087)
                     /locus_tag="VGC27_07770"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY0282504.1"
                     /translation="MNGKERFRLDARARRQELAGAISEYAWQLATRRADYFDGFGFAA
                     GAVVAGYWPFREEADPRPLMEALAAKGHPLALPRVTRRTAPLEFHRWIEGDPMRTNAF
                     EIAEPLANTEIMTPSIVLVPMLSFDASGHRLGYGGGYYDRTLAMLRAAGRVLAVGIAY
                     AGQEVAAVPRRGHDEPLDAIVTEKGFRRFHQT"
     gene            complement(1100..1257)
                     /gene="ssrS"
                     /locus_tag="VGC27_07775"
     ncRNA           complement(1100..1257)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGC27_07775"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1271..1630)
                     /gene="zapA"
                     /locus_tag="VGC27_07780"
     CDS             complement(1271..1630)
                     /gene="zapA"
                     /locus_tag="VGC27_07780"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY0282505.1"
                     /translation="MPLVNVMVNGRAYTIACDDGEEEHLKQLASHVDSKVRELLGSVG
                     QVGDQRLMLMAALLLADEYHTVMTQRDALKAELAGLAGVRDELDKRLNKSESFSADSL
                     EAAAKRIENIAAKLSRA"
     gene            complement(1736..1999)
                     /locus_tag="VGC27_07785"
     CDS             complement(1736..1999)
                     /locus_tag="VGC27_07785"
                     /inference="COORDINATES: protein motif:HMM:NF025122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HEY0282506.1"
                     /translation="MSRLELAAERLGKALELLDETAAPLTKARVSAAGAEKRIAHLDE
                     EREKLLVRIAELEQEVHALSGVTQEVEDRLDGAIEEIRSALGR"
     gene            2153..>3889
                     /gene="tkt"
                     /locus_tag="VGC27_07790"
     CDS             2153..>3889
                     /gene="tkt"
                     /locus_tag="VGC27_07790"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0282507.1"
                     /translation="MSSQNTDSSHPAAGGEEQTKVRRLANALRMLTMDAVEAAKSGHP
                     GLPMGMADTATVLFSKFLKFDPGQPNWPDRDRFVLSGGHGSMLLYSLLYLTGYPGITL
                     DDIKHFRKLESPCAGHPEYGHIPGIEVTTGPLGQGLAHAVGMALAERILNARFGDGLV
                     YHKTYVIASDGDLMEGVSHEAISLAGHLKLSRLIVLYDDNGISIDGPTSISDSTDAVE
                     RFQAAGWNTCRVDGHDVADVARALAWAQTSDKPVLLACKTIIGFGFPTRAGTQKAHSD
                     APGAEEIAGARKILSWPYEPFVIPDDVAEGWRAIGARGQGAYEAWTKRKREAAPALAA
                     AFDAAIAGEVPAALDAGLAALKKKISAEKPSVATRKASEMALDVVNANWPITIGGSAD
                     LTPSNNTKTKENMKDIVTGDFSGRYVHYGIREFGMAAALNGMALHGGIVPYGGTFMVF
                     SDYCRPAIRLAALMKIRVVYVMTHDSIGLGEDGPTHQPVETLAAVRAIPGLLVFRPAD
                     AVETAECWALALADKKRPSFMALCRQNLPTVRTTHSDENMCAKGAYELAGAANAKVTL
                     LSTGSEVELALKA"
BASE COUNT          719 a         1270 c         1202 g          698 t
ORIGIN      
        1 ggtacgaccc cgccctgctc aaggagcgga ttggtccgtc cgggcagaag aggagcatcg
       61 cggaccgcat cctgatgccg ctgctgggcg tggtctattg cgcctggctg gtattcatgg
      121 cgctggatgt gcgatggcac gggttctcgc agatgcccga atggctgaat tacgcggcgg
      181 gcgccgccat cctcgccagc ttcgccggca tggtgcgggt gttccgcgaa aacagttttg
      241 cgtccagtgt cgtgagggtc cagaaagacc gcggccagac agtcatcacg acagggcctt
      301 atgcgatcgt gcgccatccc atgtacagca tcgccagttt cacctatctc gtcattccgt
      361 ttgcactggg atcgtggatg gggcttctgg ccgttccgct gctgatcctg ggacttgcca
      421 tccgcgccct tgtcgaagag cgcgtcctca aggaacagct tgaaagctat ggcgactata
      481 tggcgaaggt gcgctaccgc ttcatgccct atgtctggtg aaaacgccgg aagccctttt
      541 cggtgacgat ggcgtccagc ggctcgtcgt ggccgcggcg tggcacagcg gccacttcct
      601 gccccgcata ggcgatgccg acggcgagga ccctgcccgc ggcgcgcagc atcgccagcg
      661 tacggtcgta atagccgccg ccatagccca gcctgtggcc cgacgcatcg aaggacagca
      721 tcggcaccag cacgatggag ggcgtcatga tttcggtgtt ggccagcggc tcggcgattt
      781 cgaaagcgtt cgtccgcatc ggatcacctt cgatccagcg gtggaattcg agcggcgcgg
      841 tgcgccgggt gacgcgcggc aaggccagcg gatggccttt cgcggcgagc gcttccatca
      901 gcggacgcgg atcggcttcc tcgcggaacg gccaatagcc ggcgacgacg gcgccggcgg
      961 caaagccgaa gccgtcaaaa tagtccgcgc ggcgggtggc gagctgccag gcgtattcgg
     1021 agatcgcgcc tgccaactct tgacgccgcg cgcgggcgtc gaggcgaaaa cgttctttgc
     1081 cgttcatggt catcaagcag ataggaccgc caccgccgtt ggatccggat cctcacgggc
     1141 ctgcaagtgc aggtgggcgc catgtgtccg cagccacggc cacggtcagg gacagctccc
     1201 ggtgagtacc attaaggccc ctgggcatcg ctctcctgac gcgcggggca gtacctatcc
     1261 aggaaccatc ctacgcgcgc gacagcttgg cggcaatgtt ctcgatccgc ttggcggcgg
     1321 cttccaacga atccgcggag aaactctcgg atttgttcaa tcgtttgtcc aactcgtcgc
     1381 gcacaccggc aaggccggca agctcggcct tgagcgcgtc gcgttgcgtc ataacggtgt
     1441 ggtattcgtc ggccaggaga agcgccgcca tcaacatcag gcgctggtcg ccgacctgcc
     1501 caaccgaacc gagtagctcg cgcactttgc tatccacatg ggaagcgagc tgcttgagat
     1561 gctcttcctc gccatcgtcg caagcgatgg tgtaagcgcg tccgttcacc atgacgttga
     1621 cgaggggcat tgcaatctcc gtgtccaaaa gcgaatagcg aatggcgagt agcgcaagat
     1681 aattttccta tcttcttctc cctattcgct actcgctatt cgctattcgc cagcttcacc
     1741 ttcccagcgc cgagcggatt tcctcgatgg cgccgtcaag gcggtcctcg acttcctgcg
     1801 tcacgcccga caaagcgtgt acttcttgct ccagctcggc gatgcgcacc agcagctttt
     1861 cgcgctcttc gtccagatgc gcgatgcgct tttcggcccc cgccgcggaa acacgcgcct
     1921 tggtcaatgg cgctgcggtt tcgtcgagca attctaaagc cttaccgagg cgttctgccg
     1981 cgagttccag cctgctcatg agagcgcctc cttcttcgtg tcagggcaag accttaggcg
     2041 catgagcgaa atagggtcaa cgcaaagtcc tagaagttct tggggagaga ccttgacggc
     2101 aaagccccgc cctcgttaag gccgagcagc tagccgaaag gtaaaccaga atatgagcag
     2161 ccaaaacacc gattcctccc accccgcggc cgggggcgaa gagcaaacca aagtccgccg
     2221 tctggcgaac gcgctgagaa tgctgacgat ggacgccgtg gaagccgcca agtcgggcca
     2281 tcccggcctg ccgatgggca tggccgatac cgccaccgtg ctgttctcca aattcctgaa
     2341 gttcgatccg ggacagccga actggcccga ccgcgaccgt ttcgtgctct ccggcggaca
     2401 cggctcgatg ctgctctatt cgctgctcta tttgaccggc tatcccggca tcacgctcga
     2461 cgacatcaag catttccgca agctcgaaag cccctgcgcc ggccaccccg aatacggcca
     2521 tatccccgga atcgaagtga ccaccggccc tctcggccag ggcctcgcgc acgccgtcgg
     2581 catggcgctg gccgaacgca tcctcaacgc gcgcttcggc gacggactgg tctatcacaa
     2641 gacctatgtg atcgccagcg acggcgacct gatggaaggc gtcagccacg aagcgatctc
     2701 gctcgccggc catttgaaac tgtcgcgcct gatcgtgctc tacgacgaca acggcatttc
     2761 gatcgacgga ccgacctcga tctccgactc gacggatgcg gtcgagcgct tccaggccgc
     2821 gggctggaac acctgccgcg tcgacggcca tgacgtggcc gacgtggcgc gcgcgctcgc
     2881 ctgggcgcaa accagcgaca aaccggtgct gctcgcctgc aagacgatca tcggcttcgg
     2941 ctttccgacc cgcgccggga cgcaaaaagc gcacagcgac gcgccgggcg ccgaggaaat
     3001 cgccggcgcg cggaaaatcc tgagctggcc ctacgagccg ttcgtcattc cggacgacgt
     3061 tgccgagggc tggcgcgcca tcggcgcgcg cggccaaggc gcctatgaag cctggaccaa
     3121 gcgcaagcgc gaagcagccc ctgccctcgc cgccgcgttc gatgcggcga tagcgggcga
     3181 ggttcccgcc gctctcgatg ccggactcgc cgcgttgaag aagaagataa gcgccgagaa
     3241 gccttcggtc gccacgcgca aggcatccga gatggcgttg gacgtcgtca acgcgaattg
     3301 gccgatcacc atcggcggtt cggcggacct caccccctcc aacaacacca agaccaagga
     3361 aaacatgaag gacatcgtga ccggcgattt ctccggccgc tatgtccatt acggcatccg
     3421 cgagttcggc atggcggcgg ccctcaacgg catggcgctg catggcggga tcgttcccta
     3481 cggcggcacc ttcatggtgt tctcggatta ttgccgcccg gcgatccggc tggcggcgct
     3541 gatgaagatt cgtgtcgttt atgtgatgac ccacgattcc atcggcctgg gcgaagacgg
     3601 ccccacccat cagccggtcg aaaccttggc ggcggtgcgc gccataccgg gattgctcgt
     3661 cttccgcccg gcggatgcgg tggaaaccgc ggagtgctgg gcgctggcgc tggccgacaa
     3721 gaagcgtccg agtttcatgg cactgtgccg ccagaacctc cccaccgtgc gcacgaccca
     3781 cagtgatgaa aatatgtgcg ccaaaggcgc ctatgaattg gcgggcgcgg caaacgccaa
     3841 ggtgacgctg ctgtccaccg gctcggaagt agagctggcg ctcaaggcg
//
LOCUS       DASWPU010000360         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_27297,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000360 DASWPU010000000
VERSION     DASWPU010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27297"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..321)
                     /locus_tag="VGC27_07795"
     CDS             complement(61..321)
                     /locus_tag="VGC27_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282508.1"
                     /translation="MIPLLMILRVRTESGHSVRLWLPLFLLWLLVLPLLLLLLPFYLI
                     FCIAARVNPGRWIAAPWALLSASAGTHIEVVTPRAFVFIHVY"
     gene            complement(318..686)
                     /locus_tag="VGC27_07800"
     CDS             complement(318..686)
                     /locus_tag="VGC27_07800"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HEY0282509.1"
                     /translation="MADRDWQELVALTQGRPLVVERVRLQDSDIAVEGAFELPQLARL
                     SAEDQIFVAAFVKSHGSIKEMERVFGVSYPTIKARLNRIAARLEFVDVDPAPSRSEIL
                     DRLERGEIGADEAVRALEGK"
     gene            924..1481
                     /locus_tag="VGC27_07805"
     CDS             924..1481
                     /locus_tag="VGC27_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HEY0282510.1"
                     /translation="MGFQIKKAAQVVAFFARERGGSIDIIDASKLVYLGDREFMDRFG
                     LTILNDVFYSMEQGPICSNTYDLMKGKGPPEERKIWEHYVTPRQGNVLRSTKAFEDED
                     LDELSDAELEALYAIAERFAEFQPFELVRWVHETCKEWKNPGKTSTYLPFADVFKALC
                     KENNKELERRLVGLRKLSFEVEKAK"
     gene            complement(1890..>2127)
                     /locus_tag="VGC27_07810"
     CDS             complement(1890..>2127)
                     /locus_tag="VGC27_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282511.1"
                     /translation="YLGAISHTLTAAGALAQAGVRIAALVVNDSGDGAVPMEDTVAAL
                     ARFLPGISITTISRAPEDAVESFARLAERLTSST"
BASE COUNT          504 a          569 c          601 g          453 t
ORIGIN      
        1 atcttccctt cggcaagcat ttgcaggatc tgtttgcggt tctcgttcac gtgacttctc
       61 ctagtagaca tggatgaaaa cgaaagcgcg cggcgtgacc acttcgatgt gggtgccggc
      121 cgacgcgctc agcaaggccc atggcgcggc gatccagcgg cccggattca cccgcgccgc
      181 gatgcagaaa atgagataaa acggcagcag cagaagcagc agcggcaaca ccagcagcca
      241 cagcaggaac agcggcagcc acagccggac gctgtgcccg ctttccgtcc gtaccctgag
      301 gatcatcagc agcgggatca tttgccctcc agcgcgcgga cggcctcgtc ggcgccgatc
      361 tcgccgcgct ccaaccggtc gaggatttcg ctccgcgaag gcgccggatc gacgtccacg
      421 aattccaggc gcgcggcgat gcggttcagc cgcgccttga tggtgggata ggagacgccg
      481 aagacccgct ccatctcctt gatcgaaccg tggctcttga cgaaggcggc gacgaagatc
      541 tggtcctcgg cggaaagccg ggccagttgc ggcagctcga aggcgccttc cacggcgatg
      601 tcgctgtcct ggaggcggac gcgctccacc accaaaggac ggccctgagt cagggcgaca
      661 agttcctgcc agtcacggtc ggccatgggt aaacctgcga aatctcattc gcagtgagat
      721 atagattgat attttcaatt tttcaatatc gcaatattag aaaaatcaat ttttcttcaa
      781 aatcagcccc ctcatctggc ttggcacttg agataattag aaaaatgact tcgtgtcatt
      841 ttttggccct tatcgcgcgt atgataagtt tgttgagtcg gaggagcgtt gtgccgagct
      901 ggagtaggca cgaggcaaag gacatgggct ttcaaatcaa gaaggcagcg caggtcgtag
      961 cgttcttcgc gcgagagcgt gggggctcta ttgacatcat tgacgcgtcg aagcttgtgt
     1021 atctcggcga ccgggagttt atggatcgtt ttgggctaac aatcctaaac gacgtgttct
     1081 actccatgga gcaggggccc atttgctcca acacctatga tcttatgaag ggtaaaggac
     1141 cgcccgaaga aagaaagata tgggaacatt acgtcacccc tcgccaaggt aatgttctta
     1201 ggtccacgaa ggcttttgaa gacgaagatt tggatgaact cagcgatgcg gagttagaag
     1261 cactttatgc gattgccgaa agatttgcgg aatttcagcc gtttgaactc gtaaggtggg
     1321 ttcacgaaac ctgcaaagag tggaaaaatc ctggaaaaac ctcgacgtac cttccatttg
     1381 cggatgtgtt caaggcgctt tgcaaagaaa acaacaagga acttgaacgg cgtcttgtag
     1441 ggttacgcaa gctcagtttc gaagtcgaaa aggcaaagtg agctttactc ccgcaaagaa
     1501 gcaaacgctg cttatcccgt ccgggtccga gcatcacctt ttcttcgttg tgacagatcg
     1561 ctgtagcgcc gggtgccatt tattggtcaa tctttcgtca attgaaccaa gcattgacca
     1621 cgataacact tgcgagcttg gactaggcga ccatcccttt gttaagcagc caagtttaat
     1681 catgtatgga atggctgaga aactggatca cgaaatcctt atcgcaaaag tgaataatgg
     1741 ccgctacaag ccaatggacc ccgttggcga cgacgttttt gatagaatct gcgccggaat
     1801 actcaagtcg ccattcacga agcccgcgat caagaactac tatcgccagg tgacagggct
     1861 tctcgtggtc gtatccaaac ggaaaataat taagtcgacg aagtaagacg ctctgccagt
     1921 cgggcgaagc tttcgacggc gtcctccggc gcgcgggaga tggtcgtgat ggagatgccg
     1981 gggaggaacc gagctagcgc cgccaccgtg tcttccatcg gcaccgcgcc gtcgccgctg
     2041 tcgttgacca cgagggccgc gattctcacc cccgcctgcg ccagcgcgcc tgccgcggtc
     2101 agcgtgtggc tgatggcgcc gagataa
//
LOCUS       DASWPU010000361         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_88431,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000361 DASWPU010000000
VERSION     DASWPU010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88431"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..485)
                     /locus_tag="VGC27_07815"
     CDS             complement(81..485)
                     /locus_tag="VGC27_07815"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY0282512.1"
                     /translation="MRYIALIDGKAGAYGASFPDLPGCTVMGKTIDAAMNNAAEALRD
                     WVEVAEESGVRIPAPCAAETVRRDGGVKQALAAGAQLASVPLVRETGKPVKANLSLDS
                     GVLAALDHEADRRSLTRSALIELMAREMLVKM"
     gene            complement(522..716)
                     /locus_tag="VGC27_07820"
     CDS             complement(522..716)
                     /locus_tag="VGC27_07820"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEY0282513.1"
                     /translation="MPPAETSRAKVAARLGREGWELVRHGANHDVFRHPQKPAVIVLP
                     RHRILSPGVARSIAKAAGWS"
     gene            1064..>1608
                     /locus_tag="VGC27_07825"
     CDS             1064..>1608
                     /locus_tag="VGC27_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282514.1"
                     /translation="MQSVKTLALWGVLAPCFSLALSGAALAQTIPSSPAEHAQTEQLN
                     QNITDNNAAAIAQSEQANAEYQAQQLQYQQQLQQYRASRQNYEERSARYYAARDRYVS
                     AHARYHRAAWPASYQHGIIVYRAELLGARVQTYNGHTIGRVEELALANGRVDAVRVTL
                     DNGRGDVWIESADLRFDANKGV"
BASE COUNT          321 a          545 c          465 g          277 t
ORIGIN      
        1 ggcttccgcc ttcgcgctgc gcgcttcggc ggacaggtcg gcaagataat tgttctgcaa
       61 aaaatacgaa ggggtgccct ctacatcttc accagcattt cgcgcgccat gagttcgatg
      121 agcgcggagc gggtgaggct gcgccggtcc gcttcgtggt cgagagcggc aagcacgccg
      181 gaatcgaggg aaagattcgc cttcaccggc ttgcccgttt cgcgcaccag cggcacggag
      241 gcaagctgtg ctcccgccgc aagcgcttgc ttcacgccgc cgtcgcggcg gacggtttcc
      301 gccgcacacg gcgccggaat cctgacgccg gattcctccg caacctccac ccaatcgcgc
      361 aacgcctcgg cggcgttgtt catcgcagcg tcaattgttt ttcccatcac ggtgcacccc
      421 ggaaggtccg gaaacgaagc gccataagcg cccgccttgc cgtcgatcaa cgcaatataa
      481 cgcatagcgg ttctccggtt ccgtcgaata tcaaaacaaa ctcaactcca ccccgcggct
      541 ttggcgatgc tgcgggccac gccgggagaa agaatgcgat gcctgggcag cacgatcaca
      601 gccggctttt gcgggtgacg gaacacatca tggttcgcgc cgtgccgcac cagttcccat
      661 ccttcgcgac caagcctcgc ggcaaccttg gccctgctgg tttcggccgg cggcatcgtc
      721 ttacgtctct cagtgcgcct atataggcgc attcagcgac gccgacaagt ggttacgcgc
      781 cgaatggcta atttgagtta aactcccgcc gcttggcccg ccgcgaaggt aatgcggccc
      841 gcctttcccg cccatttgtg cgggcggcga tcaaatgcag tccggcggcg ttcaccatcc
      901 gcctgagtag attccgaacc tgccctgcgg caagcggcgg tggcagtgtc cgcgggagcg
      961 atcatcggcc ggaaaagccc gcgctacgcg gcatttctgc cgcacgacgg aatcaacacg
     1021 ccgatatcgc gtctccatca agcggaaaaa aggaaccaca atcatgcaat ctgtcaaaac
     1081 tctcgccctt tggggcgtgc tggcgccgtg cttttcgctg gccctttcgg gcgcggcgct
     1141 cgcccagacc atcccctcct ccccggcgga acatgcccag acggagcagc tcaatcagaa
     1201 catcaccgac aacaatgccg ccgccatcgc gcaaagcgaa caggccaacg ccgaatatca
     1261 ggcccagcag cttcaatacc agcagcagct gcaacaatac cgggcgagcc ggcagaatta
     1321 cgaagaacgc tccgcgcgct attacgcggc gcgcgaccgc tacgtctcgg cgcacgcgcg
     1381 gtatcatcgc gccgcatggc ccgcaagtta ccagcatggc atcatcgtct acagagccga
     1441 gctgttggga gcgcgcgtcc aaacctacaa cggccacact atcggccgcg tggaagagct
     1501 tgcgctggcc aacggtcgcg tcgacgcggt gcgcgtcaca ctcgacaacg gcagaggcga
     1561 cgtctggatc gaatccgccg atctgcggtt cgacgccaat aaaggggt
//
LOCUS       DASWPU010000362         4694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_15316,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000362 DASWPU010000000
VERSION     DASWPU010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15316"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /gene="lysA"
                     /locus_tag="VGC27_07830"
     CDS             complement(<1..1251)
                     /gene="lysA"
                     /locus_tag="VGC27_07830"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY0282515.1"
                     /translation="MNHFSYKNGRLHAEDVDLADLAAAIGTPFYCYSTATLERHYRVF
                     AEAFPVGTLVAFSVKANGNLAVLKTLGRLGAGADVVSGGELRKALAAGIAADKIVFSG
                     VGKTKDEMRLGLEAGIYQFNVESEPELAALNEVAAAMGKRAPIAFRINPDIDAKTHAK
                     ISTGGAETKFGIPWSRARAAYGLAATLKNIEIVGVDVHIGSQITELAPFEAAFTRVVE
                     FVGELRRDGYVLSRIDLGGGLGVPYRSDKAPPPEPAAYAAMAVRLTRGLKAQLIMEPG
                     RMIAANAGILVARVIYAKTGERKRFLILDAGMNDLMRPALYDAYHDVVPVEEPKPDAP
                     RERTDVVGPICETTDIFAADRDLPRLKDGDLVAILTAGAYGAVMSSAYNARPPAPEIL
                     VKGTEWAVVRPRMDDDAHLETDRIP"
     gene            complement(1248..1400)
                     /locus_tag="VGC27_07835"
     CDS             complement(1248..1400)
                     /locus_tag="VGC27_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282516.1"
                     /translation="MRTALRLALAFAVLLCASGCGVKSDLLKPDGHPTQKGDKDPSKP
                     PTPIGR"
     gene            complement(1397..2779)
                     /gene="argH"
                     /locus_tag="VGC27_07840"
     CDS             complement(1397..2779)
                     /gene="argH"
                     /locus_tag="VGC27_07840"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY0282517.1"
                     /translation="MVTNKMWGGRFAGGPAKIMEDITPSIDFDKRLAEQDLAGSRAHA
                     RMLAVQGIISKDDADAILKGLDAVGREIAEGKFAFQRALEDIHLNIEARLAEIAGPAA
                     GRLHTARSRNDQVATDFRLWLREACARADDGLDALQSALLAQAEKHAATVMPGFTHMQ
                     SAQPVTFGHHCLAYVEMFGRDRSRFADAAKRLNECPLGAAALAGTSFPIDRDAVALAL
                     GFARPMRNSLDAVSARDFALEYLAAAAISATHLSKLAGELVQWCSSAFGFVRLTDAFT
                     TGSSIMPQKRNPDAAELIRGKTGRVLGDFVALATVVKGLVLAYGSDLQEDKERVFDAA
                     DTLELSLAAMAAMIADLEAQPEAMRAAASAGYPTATDLADWLVRVQKKPFREAHHAAG
                     AIVKRAEELRFDLDKLPLAEMQKIEPSITEDVFAVLSLDASVASRLSLGGTAPARVRE
                     QIAFWREKLK"
     gene            3062..3628
                     /locus_tag="VGC27_07845"
     CDS             3062..3628
                     /locus_tag="VGC27_07845"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0282518.1"
                     /translation="MTLPRFRPLTIAVAIGAAVLLAVLYGITTLSVHAPPASLQPLKL
                     THPAKPVPAVVFAGADGRLHTLAEFKGRLVLLNLWAPWCAPCVKELPALAALQKALPR
                     DRFAVVAVDVGRDTLAEAGAFLAEHDARALTAYLDSNTSLFRIFGAYGLPLSILIDAE
                     GREIGRVEGPADWSAPDSVRYLKRLAGG"
     gene            3683..3997
                     /locus_tag="VGC27_07850"
     CDS             3683..3997
                     /locus_tag="VGC27_07850"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY0282519.1"
                     /translation="MGNVAVAGFDWDEGNLTKCAKHGVTVEEIEAFFLAGPAVAPDPA
                     HSALEDRLIAVGRTAQGRALFVAFTLRQKQDRRLIRPVSARYMHKKEIKAYEKESSQT
                     QK"
     gene            3966..4217
                     /locus_tag="VGC27_07855"
     CDS             3966..4217
                     /locus_tag="VGC27_07855"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HEY0282520.1"
                     /translation="MKKRVPKLKSDKDAEAFLDQDLSELDFSQFKPARFEFEKKTARV
                     NMRLPQPLLDAVKKRATSRKIPYQRFIREALEQAVALRK"
     gene            complement(4225..>4694)
                     /locus_tag="VGC27_07860"
     CDS             complement(4225..>4694)
                     /locus_tag="VGC27_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0282521.1"
                     /translation="IGLHFMNPVPVMQLVEVIRGIATDDATFQAALEIIKRVGKTAAY
                     AEDFPAFIVNRILLPMINEAVYTLYEGVGNVEAIDTAMRLGANHPIGPLQLADFIGLD
                     TCLSVMQVLYEGLADSKYRPCPLLVKYVEAGWLGRKTGRGFYDYRGEHPVPTR"
BASE COUNT          802 a         1499 c         1528 g          865 t
ORIGIN      
        1 gggaattcgg tcggtttcga ggtgcgcgtc atcgtccatt cggggccgca ccacggccca
       61 ttctgtgccc ttgaccagga tttccggggc gggcggacgg gcattgtagg ccgatgacat
      121 gaccgcgcca taggcgccgg cggtcaaaat cgccaccaga tcgccgtcct tcagccgggg
      181 cagatcgcgg tccgccgcga agatatccgt ggtttcgcag atcgggccga ccacgtcggt
      241 gcgttcgcgg ggcgcgtccg gcttcggctc ctcgaccggc acgacatcgt gataggcgtc
      301 gtagagcgcc ggccgcatca gatcgttcat gccggcgtcg aggatgagga accgcttgcg
      361 ttcgccggtc ttggcataga tcacgcgcgc caccagaatt ccggcattgg cggcgatcat
      421 ccgtcccggc tccatgatca gttgcgcctt caatcctctg gtaagcctta ccgccatggc
      481 ggcatacgcg gcgggttccg gcggcggcgc cttgtcgctg cggtagggca cgcccaggcc
      541 gccgcccaga tcgatgcggg agaggacgta tccgtcgcgg cgcagttcgc ccacgaattc
      601 gaccacgcgt gtgaaggccg cctcgaacgg agcgagttcg gtgatctggc tgccgatatg
      661 gacatcgacg ccgacgatct cgatattctt cagtgtcgcc gccaggccat aggctgcgcg
      721 cgcacggctc cagggaatgc cgaatttggt ctccgcgcct ccggtcgaga ttttcgcatg
      781 cgtcttggcg tcgatgtcgg gattgatgcg gaaggcgatg ggcgcgcgct tgcccatggc
      841 agcggccacc tcgttcagcg ccgccaattc gggttcgctt tcgacgttga actgatagat
      901 gccggcctca agccctaggc gcatttcgtc tttggtcttg cccacgcccg agaacacgat
      961 cttgtcggcg gcgatgccgg cggccagcgc ctttcgcagt tcgccgcccg acaccacgtc
     1021 ggcgcccgcg ccgagcctgc ccagcgtctt cagcaccgcc agattgccgt tggccttgac
     1081 cgagaacgcg accagcgtgc cgactggaaa ggcctcggcg aagacgcggt aatggcgttc
     1141 cagcgtcgcc gtcgaatagc agtagaacgg cgtgccgatg gcggcggcca gatccgcaag
     1201 atcgacgtcc tcggcgtgga gccggccgtt tttgtaggaa aaatgattca tcggccgatc
     1261 ggcgtaggcg gtttggaagg gtccttgtcg cctttttgcg tcgggtgacc gtcgggcttg
     1321 agcaaatcgc ttttgacgcc gcagccggaa gcgcaaagca ggacggcgaa cgcaagcgcc
     1381 agacgcaggg ctgttctcat ttcaatttct cccgccagaa agcgatctgc tcgcgcaccc
     1441 gcgcgggcgc ggtgccgccc aggctgaggc gcgacgccac gctggcatcc aggctgagga
     1501 ccgcgaacac gtcttcggtg atggaaggct cgatcttctg catttcggcc agcggcagct
     1561 tgtcgagatc gaagcgcagt tcttcggcgc gcttgacgat ggcgcccgcg gcgtgatggg
     1621 cctcgcggaa cggcttcttc tgcacccgca ccagccagtc ggccagatcg gtcgcggtcg
     1681 gatagcctgc gctcgcggcg gcgcgcatcg cctcgggctg cgcctcaagg tccgcgatca
     1741 tcgccgccat ggcggccaac gagagctcca gcgtatcggc ggcgtcgaag acgcgctcct
     1801 tgtcttcctg caagtcgctg ccataggcca gcaccaggcc cttgaccacg gtcgccagcg
     1861 ccacgaaatc gcccagcacc cggccggtct tgccgcggat cagttcggcg gcgtcggggt
     1921 tgcgcttctg cggcatgatc gaggagccgg tggtgaaggc gtcggtgagg cgcacgaagc
     1981 cgaaggcgga ggagcaccac tgcaccagct cgccggcaag tttcgacaga tgcgtcgccg
     2041 aaatcgcggc ggccgccaga tattccagcg cgaagtcgcg cgccgaaacc gcatcgaggg
     2101 aattcctcat cggccgcgca aagccgagcg ccagggctac ggcgtcgcgg tcgatgggga
     2161 acgaggttcc cgccagcgcc gccgccccca gcgggcattc gttcagccgc ttggcggcgt
     2221 cggcgaagcg gctgcggtcg cgcccgaaca tttccacata ggccaggcag tgatggccga
     2281 aggtgaccgg ctgggcgctc tgcatatggg tgaagccggg catgacggtg gcggcatgtt
     2341 tttccgcctg ggccagcagt gcactttgca gcgcgtccag gccgtcgtcg gcgcgggcgc
     2401 aggcctcgcg caaccacagc cggaagtccg tcgccacctg atcgttgcgc gagcgggcgg
     2461 tgtgcagcct tcccgccgcc gggcccgcga tttcggccag tcttgcctcg atgttcagat
     2521 ggatgtcttc cagcgcccgc tggaaggcga atttgccctc cgcgatctcc cggccgacgg
     2581 cgtcgaggcc cttgaggatt gcgtccgcgt cgtccttgga aataatgcct tgaacggcca
     2641 acatgcgcgc atgcgcgcgg gagccggcca ggtcctgctc ggccaggcgt ttgtcgaaat
     2701 cgatggaggg cgttatgtcc tccatgatct tggcgggccc gccggcgaac cggccgcccc
     2761 acatcttgtt cgtaaccata agagcctgtt ttgatgatcc tcatgagtcg cgtttcgtgc
     2821 gaaaatcgca ctatggcagg agaagcgcga agagcgtatc aggaaatacg gtcgagcgct
     2881 tcgacgcacc agagagcgat tttcgccgaa accctccggg acgcgccgct tttgcccgcc
     2941 cggtttgtcg ccgcgcttgc ccgatgtccc cgcatcgcgc cgcgcgcggc tcctgccgga
     3001 aatcgggcaa aatcggcagc gtcgcggcca tgacgatcat caaagcaggc tctgaacgcc
     3061 catgacgctg ccccgttttc gccccctcac cattgcggtt gcgatcggcg cggccgtgct
     3121 tctggctgtt ctatatggga tcacgacgct ttctgtccac gcgccgccgg cctcgctcca
     3181 gccgctcaag ctgacccatc cggccaaacc cgtgcctgcc gtggtttttg ccggggcgga
     3241 cggcaggctg cacactttgg ccgagttcaa gggccgcctg gtgctgctga acctgtgggc
     3301 accctggtgc gcaccctgcg tcaaggagtt gccggcgctg gcggcgctgc aaaaggcgct
     3361 gccgcgggac cgcttcgcgg tggtggcggt ggatgtcggc cgcgacacgc tggcggaggc
     3421 cggcgctttc ctggccgaac acgatgcgcg cgcgcttacc gcctatctcg attcgaatac
     3481 cagcctgttt cgcattttcg gcgcttacgg gctgccgctc agcatcctga tcgacgccga
     3541 gggccgcgag atcgggcgcg tcgagggacc cgcggactgg tccgcgcccg attccgtgcg
     3601 ttatctgaaa cggctggccg ggggctgacc gcccagacat gcgtattgac ttttgtatgt
     3661 acaaatgtca gacttccacg ccatgggaaa cgtcgctgta gccggtttcg actgggacga
     3721 aggcaatctt acgaaatgcg ccaagcacgg cgttacggtc gaggagatag aggcgttctt
     3781 tctcgccggg ccggcggttg cgcccgatcc ggcgcattcg gcgcttgaag acagactgat
     3841 tgccgtgggg cggaccgcgc aaggccgggc gcttttcgtg gctttcacct tgcgtcagaa
     3901 gcaagaccgc aggctcatcc ggccggtcag cgcacgctac atgcacaaga aggaaatcaa
     3961 ggcttatgaa aaagagagtt cccaaactca aaagtgacaa agacgccgag gcattcctcg
     4021 atcaggacct ctcggagttg gatttctcgc agttcaaacc cgcacgcttt gagttcgaaa
     4081 agaagacggc gcgcgtgaac atgcgtcttc cgcaaccctt gctggatgcg gtgaagaagc
     4141 gggcaacgtc acgcaaaatt ccttatcagc gcttcatccg cgaagcgttg gaacaggcag
     4201 tcgcattacg aaagtagaat ttgcctaccg cgtcgggacc gggtgttccc cgcgataatc
     4261 gtagaagccg cggccggtct tgcgtcccag ccagccggct tcgacatatt tcaccagcag
     4321 cgggcagggc cggtacttgg aatcggccag gccctcatac agcacctgca tcaccgacag
     4381 gcaggtgtcc agcccgatga aatcggccag ttgcagcggc ccaatcggat ggttggcgcc
     4441 cagccgcatg gcggtgtcga tggcttcgac gttgccgacc ccttcgtaca gcgtatagac
     4501 cgcttcgttg atcatcggca gcaggatgcg gttgacgatg aaggcgggga aatcctcggc
     4561 ataggcggcg gtcttgccga cgcgcttgat gatctcgagc gccgcctgga aagtcgcatc
     4621 gtcggtggcg atgccgcgga tgacttccac cagttgcatc accggcaccg gattcatgaa
     4681 atgcagaccg atga
//
LOCUS       DASWPU010000363         5032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_21698,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000363 DASWPU010000000
VERSION     DASWPU010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21698"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="VGC27_07865"
     CDS             complement(<1..1382)
                     /locus_tag="VGC27_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HEY0282522.1"
                     /translation="MEPVLAEVQEETTVHATINGQAVEAVENESILHCARRYGIHIPT
                     LCELDDIDHTPGTCRVCLVEIRVPGNTNSNLATSCNTPVVEGMTVQTRSPRAREAQRL
                     QVELLLADHNQDCATCVRHGSCELQDVAQFVGLQTNRFFDPVRMAAREIDDSSPAMIR
                     DMTKCIRCQRCIAICRDGQGVDALIRTGIGQNTAVGLRSGLSQKESICVTCGQCVLVC
                     PTGALGERDETDKVLDMLYDPEIVTVFQFAPALRVAFGEEFGLPPGTNVQGQIITALR
                     LIGADIVLDTNFGADLVVMEESAELLKRLNEKRRPTFTSCCPAWINFAERHYPEILPQ
                     LSSTKSPMQALGVVAKTYLPEKMGVDPKKVRVVAIMPCTAKKDEAVRQQLQFTDMAEI
                     DTVLTTREFARLLRREGVDIARLEPSEFDNPYMSEFSGAGAIFGTTGGVMEAAVRTMY
                     YIANGKEYGK"
     gene            complement(1398..3395)
                     /locus_tag="VGC27_07870"
     CDS             complement(1398..3395)
                     /locus_tag="VGC27_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0282523.1"
                     /translation="MANIVFGVWDGAVHDHRDDHADAAETMVSLRELDTFNEGNPIRA
                     FVGDRGFFVLDTGVSLIDTFWRYMNKAAEQSCGKCTPCRVGTVLVRDALGAMRRGQES
                     TLDLDDIIALSRQITETSLCGLGQTCGRSLLAALENFRDIFEAELAAGAQPEQNGMIY
                     VTAPCIEACPSKINVPQYIDYIRDGKPEHSLGVILQKYPMAATCGRVCVRFCEMACRR
                     NLVDEAVGIKTLKRYVADYQSRTGAMSFSEDIIAESLPPSMRVAVVGAGPAGISCAYH
                     LLLHGYHVDVLEAMEQAGGMAAIGIPAYRLPKDVLKSETDIITKLGGRILFNQKLGRD
                     YSIDDLFARGYRAVFLALGCQEGTQMGVEGEDPKLAGYESGIGFLLKVHDYVAGVHKE
                     GVSGDVVVVGGGNVAMDCVRSAVRMGARIVHLVYRRSREDMPADKEEIQAAEDEGIVL
                     HFLTNPTRVLSEDGKVTGVELVHMRQTEPDERGRRKVEPVPGTERTIKCSTLIAAIGQ
                     RVLSGALKEGDGIDMNKWNCVGTDNGSLLTSRPGVFAGGDCATGPSTLIHAMAAGLKA
                     ARAIDDWVRLGEVRFFPRSRMRQILSKNRLLASDIGEIPVKSEYRVHHPELDADIRKQ
                     MFEEVERTITPEEAYREAKRCLRCYRIYSVITGQPIPESHT"
     gene            4116..>5032
                     /locus_tag="VGC27_07875"
     CDS             4116..>5032
                     /locus_tag="VGC27_07875"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEY0282524.1"
                     /translation="MSQTASWAGRFREELVGNILPFWMRHALDRQGGGFHGKIGCDGK
                     VDREAPRASVVNTRILWTFSAASRLLGNEYRETADWAYDYIIKKFWDQEYSGLYWMLD
                     HRGNPVSARKQIYAQAFGIYALTEYFRAGGNAASLNLARRLFGLIEEHSYDPVFKGYI
                     EARGREWRPLEDMRLSEKDLNCPKSMNTHLHLMEAYTNLLRVWRDPVLEARLAELLGL
                     IMDRIADASTGHFRMFFDERWNSLSDHVSFGHDIEGSWLIVEAAEVLGAADQIARARR
                     LAVAMAGAVCAQGLDRDGSVFFEADGSGAL"
BASE COUNT          915 a         1569 c         1582 g          966 t
ORIGIN      
        1 atcttgccgt attccttgcc gttggcgatg taatacatgg tgcgcacggc cgcttccatg
       61 acgccgcccg tggtgccgaa gatggcgccc gcccccgaga attcgctcat atagggattg
      121 tcgaactcgg agggttcaag cctggcgata tcgacgccct cgcgcctgag cagacgggcg
      181 aattcgcgcg tcgtcagcac ggtgtcgatt tccgccatgt cggtgaactg caattgctgg
      241 cgcaccgctt cgtccttctt ggcggtgcaa ggcatgatgg cgacgacgcg gacctttttc
      301 ggatcgaccc ccatcttctc cggcagatag gtcttggcga cgacgcccag cgcctgcatc
      361 ggcgatttgg tcgaggacaa ttgcggcagg atttcgggat aatgccgttc ggcgaaattg
      421 atccaggccg gacagcagga ggtgaaggtc ggccgccgct tctcgttcag ccgcttcaac
      481 agttcggcgc tttcctccat cacgacgagg tcggcgccga aattggtgtc gagcacgatg
      541 tcggcgccga tcaggcgcag cgccgtgatg atctggccct ggacgttggt gccgggcggc
      601 aggccgaatt cctcgccgaa ggccacgcgc aatgcgggcg cgaactggaa cacggtgacg
      661 atctccggat cgtagagcat atcgagaacc ttgtcggtct cgtcgcgctc gcccagcgcg
      721 ccggtggggc agaccagcac gcattgcccg caggtcacgc agatcgattc cttctgcgac
      781 aggccggaac gcaggcccac cgccgtattc tgaccgatgc cggtgcggat cagcgcatcc
      841 acgccctgcc cgtcgcggca gatggcgatg cagcgctggc agcggatgca cttggtcatg
      901 tcgcgaatca tggcgggaga ggaatcgtcg atctcgcgcg ccgccatgcg gaccggatcg
      961 aagaagcggt tcgtctgcag gcccacgaat tgcgccacgt cctgcaattc gcaactgccg
     1021 tggcgcacgc aggtggcgca gtcctgattg tgatcggcga gcagaagctc gacttgcagg
     1081 cgctgcgctt cgcgcgcccg cggcgagcgg gtctgaaccg tcatgccttc caccaccggc
     1141 gtgttgcacg aagtggcgag gttcgaattc gtattgcccg gcaccctgat ttcgaccagg
     1201 cagacccggc aggtccccgg cgtgtggtcg atgtcgtcaa gctcgcaaag cgtgggaata
     1261 tggatgccgt agcggcgcgc gcagtgcagg atcgactcgt tctcaaccgc ttcgaccgcc
     1321 tgcccgttga tcgtggcgtg aacggtcgtt tcctcttgga cttcggcaag aactggttcc
     1381 atgagcacgc cgctccttta ggtatgggat tccggaatgg gctgtccggt gatgacggaa
     1441 tagatgcggt agcagcgcag gcagcgcttg gcctcgcgat acgcttcctc gggcgtgatg
     1501 gtgcgttcga cctcctcgaa catctgcttg cggatatcgg catcgagctc gggatgatgg
     1561 acgcggtatt cggacttgac cggaatctcg ccgatgtccg aggccaggag tctgttcttg
     1621 ctgagaatct ggcgcatgcg cgagcgcggg aagaagcgca cctcgccgag ccgcacccag
     1681 tcgtcgatgg cgcgcgcggc cttcaggcct gccgccatgg cgtggatcag agtcgaaggg
     1741 ccggtggcgc agtcgccgcc cgcgaacacg cccggccgcg aggtgaggag cgagccgttg
     1801 tcggtgccca cgcaattcca tttgttcatg tcgatgccgt cgccctcctt gagggcgccg
     1861 ctcaacaccc gctgaccgat ggcggcgatc agcgtggagc atttaatggt gcgctcggtg
     1921 ccgggaaccg gctcgacctt gcggcggccc ctttcgtcgg gctcggtctg gcgcatatgc
     1981 accagctcga cgccggtgac cttgccgtcc tcgctcagga cgcgcgtcgg gttggtgagg
     2041 aagtgcagga cgatgccttc gtcttccgcc gcctggattt cctctttgtc ggcgggcatg
     2101 tcctcgcggc tgcggcgata gacgagatgg acgattctgg cgcccatgcg cacggccgag
     2161 cgcacgcagt ccatcgccac gttgccgccg ccgaccacta cgacgtcgcc gctgaccccc
     2221 tccttgtgaa cgcccgcgac gtaatcgtgg accttgagca gaaagccaat gccggattcg
     2281 tagccggcga gtttcggatc ttcgccttcc acgcccatct gggtgccttc ctggcatccc
     2341 agcgccagaa acaccgcgcg atagccgcgc gcgaagagat cgtcgatcga atagtcgcgg
     2401 ccgagcttct ggttgaacag gatgcggccg cccagtttgg tgatgatgtc ggtctccgac
     2461 ttcaacacgt ctttcggcag gcggtaggcg ggaatgccga tggcggccat gccgccggcc
     2521 tgctccatcg cctccaatac gtcgacatga tagccgtgca gcagcagatg ataggcgcaa
     2581 gaaatgcccg ccgggcccgc gcccacaacc gccacgcgca tggaaggagg cagcgattcg
     2641 gcgatgatat cctcggagaa actcatcgcg ccggtacggc tttgataatc ggcgacatag
     2701 cgcttcagcg tcttgatgcc gaccgcttcg tccaccagat tgcgccggca ggccatctcg
     2761 cagaagcgca cgcagacgcg cccgcaggtc gccgccatcg gatatttctg caggatcacg
     2821 cccagcgaat gttcgggttt gccgtcgcgg atatagtcga tatattgcgg gacgttgatc
     2881 ttggacgggc aggcttcgat gcagggcgcc gtcacgtaga tcattccgtt ctgctcaggc
     2941 tgcgcgcccg cggcgagttc ggcctcgaat atgtcgcgga aattctccag tgccgccagc
     3001 agcgagcgcc cgcacgtctg gcccaggccg caaagcgaag tttccgttat ctggcgcgac
     3061 aaagcgatga tgtcgtcgag atcgagcgtg ctctcctggc cgcgccgcat ggcgcccaac
     3121 gcatcgcgca ccagcacggt tcccacccgg caaggcgtgc atttcccgca ggactgctcg
     3181 gccgccttgt tcatgtagcg ccagaacgtg tcgatcaggc tgacgccggt atccagaacg
     3241 aaaaagccgc ggtcgccgac gaacgcccgg atcggattgc cttcgttgaa ggtgtcgagc
     3301 tcgcgcaggc tgaccatcgt ttccgccgcg tccgcatggt cgtcgcggtg atcgtggacc
     3361 gctccgtccc agacgccaaa aacgatgttt gccatgatct actctccaca gcggcggaca
     3421 aggcacgagc cgcacgccgg aagcgtgcct gatttctctg ccgggtaatg cgctctacgc
     3481 ggtgtctgat tagcaatacc gtcgcagcgg atatataggt atttctacga aacatgggga
     3541 gcgcgacgaa ccgcccaccc tgtctgccac gaatgcgaca tattgtttac acatttcgcc
     3601 tccggccggg taaggagcag ctccggcagc gtgataatga gtctctgttt ctagaacgac
     3661 gcgcatattg cggcggcgtg cggctccgcc ggccaacgaa cacagaaaag ccgccgccgc
     3721 tcttacggag gcgccgcttc gtccctccgg tagaaaagcg agcggccaca agatggataa
     3781 tccttctggg gccttgccaa cctggccttc tcccgggagc gctcttcctc tcatttttcc
     3841 ttaagggcag cggcaatctg tccaaaagcc ggctgtgagg ctgcgaccaa tcctgccacc
     3901 ccccgcacac cggcgccaaa actgatacaa cctattgaac acgcttaagt tttattgtgt
     3961 ccggcaaacc cgccagggcg tttttttgag cttgacgaac actgtcatga tgcaggaatg
     4021 tcagcgctgt cagagcgcaa caaagtaatt ataaatgagt gtgcccgacg ccctataggt
     4081 cgccaggaga gcccaaagac tcgcgggaag ccatcatgtc tcagacagcg agttgggccg
     4141 ggcggtttcg cgaagaactc gtgggcaaca tcctgccgtt ctggatgcgc catgcgctcg
     4201 atcgccaagg cggcggcttt cacgggaaga tcggctgcga cggcaaggtg gacagggagg
     4261 cgccgcgggc gtcggtcgtc aacacccgca tcctgtggac cttctcggca gccagccggc
     4321 tgctcggcaa cgaataccgc gagacggccg attgggccta cgattacatc atcaagaaat
     4381 tctgggacca agaatattcc ggcctgtact ggatgctcga tcatcggggc aatcccgttt
     4441 cggcgcgcaa gcagatttat gcccaggcgt tcgggattta cgcgctaacg gagtatttcc
     4501 gggccggcgg caacgccgcc agcctgaacc tcgcccgccg cctgttcggc ctgatcgagg
     4561 aacacagcta cgatccggtc ttcaaaggct atatcgaagc gcgcgggcgc gaatggcggc
     4621 cgctcgagga catgcggctg agcgaaaagg atctcaactg cccgaaatcc atgaacacgc
     4681 atctgcacct gatggaagcc tacacgaatc tgctgcgcgt gtggcgcgat ccggtgcttg
     4741 aagccaggct ggccgagttg cttgggttga tcatggaccg catcgccgat gcttccacgg
     4801 ggcatttcag aatgttcttc gacgagcgct ggaattcgct ctcggatcat gtctctttcg
     4861 gacacgatat cgaaggcagc tggctcatcg tcgaggcggc ggaagtgctt ggcgcggcag
     4921 accagatcgc gcgggcgcgc cgcctggccg tcgccatggc cggggccgtt tgcgcgcaag
     4981 gtctcgaccg ggacggcagc gtgttctttg aagccgacgg ctccggcgcg ct
//
LOCUS       DASWPU010000364        10038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_82767,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000364 DASWPU010000000
VERSION     DASWPU010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82767"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /gene="ppdK"
                     /locus_tag="VGC27_07880"
     CDS             complement(<1..2079)
                     /gene="ppdK"
                     /locus_tag="VGC27_07880"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY0282525.1"
                     /translation="MTKWVYSFGDGKADGEAKMKNLLGGKGANLAEMSNLGLPVPPGF
                     TITTEVCTYYYANKETYPSDLEKEVAAAVEKIGKQTNSKLGDPQRPLLVSIRSGARVS
                     MPGMMDTVLNLGLNPDTVEGLVKLTGNPRFVYDSYRRFVQMYSNVVLGLEHSDFEEII
                     ESAKEAKGVELDTELDTDDLKKIATEFKAQVQKKLGKPFPDDVKDQLWGAISAVFSSW
                     QVPRAKTYRRLNKIPEDWGTAVNVQAMVFGNRGETSATGVAFTRDPGTGEKLLYGEFL
                     INAQGEDVVAGIRTPQPITKIVRERQGGKKPSMEEAMPTMYAELKKIGDTLEEHFREM
                     QDVEFTIQEGKLYMLQCRTGKRTAKAALKVAIDLVSEGLIDKKTAIIRVDPAALDQLL
                     HPTLDPKAAKEEFAEGLAASPGAAVGEVTFTADEAEKRAGEGFDVILVRTETSPEDIH
                     GMHAAKGILTARGGMTSHAAVVARGMGRPCVAGAGALRIDAEAGVMTAGSVTIKRGEI
                     ITLDGTAGKIYKGRVPMRQPELTADFNKLMGWADEVRTLKVRTNADTPTDAVTAVRFG
                     AEGIGLCRTEHMFFNEDRIISVREMILSDDKPSREKALAKLLPMQRGDFEAIFKTMSG
                     LPVTIRLLDPPLHEFLPHKEAEFEDVAKACGSTVAKLRVKANALHESNPMLGHRGCRL
                     GVTFPEIYEMQ"
     gene            complement(2601..4823)
                     /gene="glyS"
                     /locus_tag="VGC27_07885"
     CDS             complement(2601..4823)
                     /gene="glyS"
                     /locus_tag="VGC27_07885"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY0282526.1"
                     /translation="MAEMPDLLLELFSEEIPARMQTQAAKDLERLIVGGLSDRGLLFE
                     GAKAFAGPRRLVLAVAGLPVKQPDVSEEKKGPRVGAPEKALEGFLRAAGVTLEQCEKR
                     SDGKGEFYVAVIARKGRETAEVLAELVPEAVAKLPWPKSMRWLPGDPVRWVRPLHSIL
                     CTFDGETVPFEFAGVKSGRATRGHRFLSQGEIEARRFDDYVRKLREAHVVLDAAERKE
                     TILHEAGQKAFALGLELIADEGLLDEVAGLAEWPVVLIGTIEDQFMDLPPEILQTSMR
                     THQKYFSLRDAKTGRMANRFAVVSNMVSADGGKEIVAGNERVLRARLSDAKFFWDQDR
                     KIRLEKRVNALKDIVFHAKVGTMQDRVDRVTFVAMVLTELLRVDFEKAKRAAWLAKAD
                     LTTGVVGEFPELQGVMGRYYALHDHEDPEVADAIRDHYKPLGPSDQVPSAPVSMVVAL
                     ADKIDNLVTLWRAGEKPTGSKDPFALRRAALGMIRIILENKLRIELQLPIFTAIAAAK
                     PRERRAWEERLRTAFTSQALFKGLADDLFTAARRHREVEEENGKGSVEGLTKADEQAI
                     AEVLEFLADRLKVALREKGTRHDLIDAVFSLGHEDDLVRLVARVEALQTFLASDDGAN
                     LLVAYRRAANILKAEEKKDKTVYDGAPDPDAFVLSEEKALYVELATAGDRVRAEIGRE
                     RFVEAMGVMSRLRQPVDAFFDKVTVNEKNPDMRRNRLLLLSRLRATLHLVADFSKIEG
                     "
     gene            complement(4816..5688)
                     /locus_tag="VGC27_07890"
     CDS             complement(4816..5688)
                     /locus_tag="VGC27_07890"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY0282527.1"
                     /translation="MAKTFQDLILTLQNYWAEQGCLILQPYDVEMGAGTFHPATTLRA
                     LGPRPWRAAYVQPSRRPKDGRYGENPNRLQHYYQYQVILKPAPEDVQELYLGSIKAIG
                     LDPALHDIRFVEDDWESPTLGAWGLGWEVWCDGMEITQFTYFQQVGGIDCDPVCAEIT
                     YGLERLAMYVQGVEFVYDLAFNENFRYGEVFHQSEREFSAFNFERADTAILFRHFEDA
                     EKECQALVEAKLALPAYDQCIKASHCFNLLDARGVISVTERAAYIGRVRALAKACCES
                     WLASPMGEYKPRYG"
     gene            complement(5763..6503)
                     /locus_tag="VGC27_07895"
     CDS             complement(5763..6503)
                     /locus_tag="VGC27_07895"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0282528.1"
                     /translation="MIATTEDRFLGGRVLVRQPVDGFRAGLDAVMLAAAVPARPGETA
                     LELGAGAGTASLCLVHRVGLISVTGIEIDPALVELATANSSANGMSVCATFAAGDALQ
                     PPGPLRRPFGHVFCNPPFHGEGASSPDAARAAALQDGGRLTDWLTGFKRVIAGGTFTA
                     ILRADRLGEALAALPERGVTVFPLWPRANAPAKRVIVQVRQGSRAPLAILPGLVLHAA
                     DGRYTPQADAVLRDGAALTLRPAQGEGG"
     gene            complement(6542..6763)
                     /locus_tag="VGC27_07900"
     CDS             complement(6542..6763)
                     /locus_tag="VGC27_07900"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY0282529.1"
                     /translation="MRAVLKTNDPVLLNFAEVLLADAGLQSVVFDGEASVMDGSIGML
                     PRRLMVADEDFVRAQAILRDGIKDAPVEP"
     gene            6883..7908
                     /locus_tag="VGC27_07905"
     CDS             6883..7908
                     /locus_tag="VGC27_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0282530.1"
                     /translation="MLASEARRSNATKDAGVSPVERLHALVSADMRATDALIHERAGS
                     TVALIPDLARHLIDSGGKRLRPLLTLAAARMGGYAGHDHVRLAAAVEFIHTATLLHDD
                     VVDESALRRGKVSANIVWGNKPSVLVGDYLLSRAFQLMVETGELAVLDILAGASAIIA
                     DGEVMQLKSTNNLAATEQHYLAVVRAKTAALFAAAAESGAVLAKRSTDYVAALRSYGD
                     NLGIAFQLVDDALDYSGRQALMGKSVGDDFREGKVTLPVILAHARADEEGRRFWRRAI
                     ETGPQGESDLDRAITLVEETGAISETMARARAYADRARQALAAVAPCETREVLADIAD
                     FCVERAY"
     gene            complement(7905..8765)
                     /locus_tag="VGC27_07910"
     CDS             complement(7905..8765)
                     /locus_tag="VGC27_07910"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY0282531.1"
                     /translation="MTTEADALHVFAPAKINLFLHVGQKRADGFHALESLVVFACTGD
                     ELAFEGAGELSLALDGPFANALTPGPDNLVLKAARALAASAGVRKGARIALTKNLPIA
                     SGIGGGSADAAATLRGLNALWKLNLAPEELQAIAAQIGSDVPVCVGSKPAWMEGRGEK
                     VTALAGIVEVPMVLVNPGVAVPTGKVFAALKERRGVGLPLPDAMKNADELVAYLKTTA
                     NDLEAPARQIAPHIGEVLDALASQPGLLLARMSGSGATCFALFDSGASAQNAAAAIRA
                     RHGNWWIVAA"
     gene            complement(8749..>10038)
                     /locus_tag="VGC27_07915"
     CDS             complement(8749..>10038)
                     /locus_tag="VGC27_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0282532.1"
                     /translation="FTLFTVALIDAWAAAGMGDMAAAEAELKGLHAQSGADALTYFNE
                     AMLAEFFGQKDKADAFYNLEMKAGGLSPRVIDAYGRFLERNKRVPEAKALYEKIAGEG
                     SFTPVANAGLARIASGRTPDALIVHAEDGAAEALFGIAASLGDASSRDVSILYLRLAL
                     YLRPNLDLAKLLLADRFEGLTKYDNAVEILRGVGTESPYYRIAAVQIAVDLVRSGKMD
                     EAIAGLTKLTAADPGEVEPWTALGDAYRQAERYADATKAYDRAILAAGEPTKKSWPLY
                     YARAIAAQQSNDWKAAEADLEMALMLSPDEPQTLNYLGYSWVDQRRNISQAVAMLEKA
                     SALAPDDGYITDSVGWAYYHLGRYADAVKALEKAVRIVPGDPTINDHLGDAYWKVGRK
                     LDAQFQWNHALAFGAEGKDKEKIEKKLEAGLPRDDRS"
BASE COUNT         1718 a         3343 c         3086 g         1891 t
ORIGIN      
        1 ctgcatttcg tagatttccg ggaaagtcac gcccaagcgg cagccgcgat ggcccagcat
       61 cgggttggat tcgtgcagcg cgttggcttt cacgcgcagc ttggcgacgg tggaaccgca
      121 agccttcgcc acatcctcga attccgcttc tttgtgcggc aggaattcat gcagcggcgg
      181 atcgagcagg cggatggtga cgggcagccc cgacatcgtc ttgaagatcg cctcgaagtc
      241 gccgcgctgc atgggaagca gcttggccag ggccttctcg cgcgacggct tgtcgtcgga
      301 caggatcatc tcgcgcaccg agatgatgcg gtcctcgttg aagaacatat gctcggtgcg
      361 gcacaggccg atgccttcgg cgccgaagcg gaccgcggtc accgcgtccg tcggagtgtc
      421 ggcattggtg cgtaccttga gcgtgcgcac ttcgtcggcc cagcccatca gcttgttgaa
      481 atcggcggtc agttcgggct gacgcatggg cacgcggccc ttgtagattt tgcccgcagt
      541 gccgtcgagg gtgatgatct cgccgcgctt gatcgtcacc gaaccggccg tcatcacgcc
      601 ggcttcggcg tcgatgcgaa gagcgccggc gccggccacg caaggacggc ccatgccgcg
      661 cgcaaccacc gccgcgtggc tggtcatgcc gccgcgcgcc gtcaggatgc ctttggccgc
      721 gtgcatgccg tggatgtctt ccgggctggt ttccgtacgg accaggatga cgtcgaagcc
      781 ctcgcccgcg cgcttctcgg cttcgtccgc cgtgaaggta acctcgccca ccgcggcgcc
      841 cggagaagcg gccaggcctt ctgcaaattc ttccttggcg gccttgggat cgagcgtcgg
      901 atgcagcaac tggtcgagag ccgccggatc aacgcggatg atcgccgtct tcttgtcgat
      961 caagccttcg ctgaccaggt cgatcgcaac cttcagcgcc gccttcgcgg tgcgctttcc
     1021 ggtgcggcac tggagcatgt aaagcttgcc ttcctggatg gtgaattcca cgtcctgcat
     1081 ttcgcggaaa tgttcttcca gcgtgtcgcc gatcttcttg agttccgcgt acatcgtcgg
     1141 catggcttct tccatcgacg gcttcttgcc gccctggcgc tcgcgcacga tcttggtgat
     1201 gggctgcggg gtacggatgc cggccacgac gtcttcgccc tgggcgttga tcagaaattc
     1261 gccgtagagg agcttttcgc cggtgcccgg atcgcgggtg aaggcgacgc cggtggcgct
     1321 ggtctcgccg cgattgccga acaccatggc ctgcacgttg accgccgtgc cccaatcctc
     1381 gggaatcttg ttgaggcggc gataggtctt cgcgcgcgga acctgccacg agctgaacac
     1441 cgcgctgatg gcgccccaca actggtcttt cacgtcatcg gggaacggct tgcccagctt
     1501 cttctggacc tgggccttga actcggtggc gatcttcttg agatcgtccg tgtccagctc
     1561 ggtatccagc tcgacgccct tggcttcctt ggcgctttcg ataatctctt cgaaatccga
     1621 atgctcgaga ccgagcacga cgttggaata catctgcacg aagcggcgat aggaatcgta
     1681 aacgaagcgc gggttgcccg tgagcttgac caagccttca accgtatccg ggttgaggcc
     1741 gaggttgagg accgtgtcca tcatgccagg catggagacg cgcgcgcccg aacgaatgga
     1801 gacgagcagc ggacgctgcg ggtcgccgag cttcgaattg gtctgcttgc cgatcttttc
     1861 gaccgccgcg gcgacttcct tctccagatc ggagggatag gtctccttgt tggcgtaata
     1921 gtacgtgcag acttccgtgg tgattgtgaa accgggagga acgggaagcc cgaggttgct
     1981 catctcggcc aggttcgcac ctttgccgcc gagcaaattc ttcattttgg cttcgccatc
     2041 ggctttgccg tctccgaacg aataaaccca tttagtcatt gtttctctac ccttttttgt
     2101 gtcttcgcgt ccgcatcgtc atagcctgcg aatgtagacc acccggccga aggctggttc
     2161 gccttaacaa acggcatccg attcttccgc gacgaatcaa cgccggagat atcgtcgcca
     2221 aggcggtctg catacgcggg ccatgacaat gaatggagtg agggactcta cctacgcttt
     2281 gatctatggg tactcaagta gggcgatgca atgtgacacg cgcgcgcgag cacggcgcat
     2341 gattattgcg tataatcaaa ttcttgttca gaccttggtc atattcaaat gcccgtccgc
     2401 caacattcgg caagccgcgt gacggggcgg ttctccgctg cggcattccg tccgctttcg
     2461 ccacaattcc gccgcatcgg ttcatgcctc tttggtaggc accctttgac ccgcctacgc
     2521 taaagctccg gcggacgagc gatgaagtac tggcctgccg tagccatgcg aaagcagcag
     2581 gctcagggtg aggaatacct ctatccctcg atcttcgaga aatccgccac caggtgcaga
     2641 gtcgcgcgca gccgggaaag cagcagcagc cggttgcggc gcatgtccgg attcttctcg
     2701 ttcaccgtca ccttgtcgaa gaaggcatcc accggctggc gcagccgcga catcacgccc
     2761 atcgcctcga cgaagcgttc gcgcccgatt tcggcgcgga cgcgatcgcc cgctgtcgcc
     2821 aattcgacat aaagcgcctt ttcttccgaa agcacgaagg cgtccgggtc gggagcaccg
     2881 tcgtaaacgg tcttgtcttt cttctcctcg gctttgagga tattggccgc ccggcgatag
     2941 gcgacgagca gattggcgcc gtcgtcggag gccagaaacg tctgcaacgc ctcgacgcgc
     3001 gccaccagcc gcaccagatc gtcctcgtgc cccaggctga acacggcgtc gatcagatcg
     3061 tgacgggtgc ccttttcgcg cagcgccacc ttcagccggt cggccaggaa ttccagaacc
     3121 tcggcaatcg cctgttcgtc cgccttggtg agaccctcca cggacccttt gccgttctcc
     3181 tcctcgacct cccggtgacg gcgcgccgcc gtgaaaagat cgtcggccaa gcccttgaaa
     3241 agagcctggc ttgtgaacgc cgtgcgaagc cgttcttccc aggcgcgccg ctcgcgcggc
     3301 ttggccgcgg cgatggccgt gaagatcggc aactgcaatt cgatcctgag tttgttctcc
     3361 agaatgatgc ggatcatgcc gagcgccgcg cggcgcagcg cgaaggggtc cttcgagccc
     3421 gtcggcttct cgcccgcgcg ccacagcgtg accagattgt cgatcttgtc ggccagcgcc
     3481 accaccatgg acacaggcgc agagggaacc tggtcgctcg gccccagcgg cttgtaatgg
     3541 tcgcgaatcg catcggccac ttcggggtct tcatggtcgt gcagcgcgta atagcggccc
     3601 atcacgcctt gcagttcggg aaactcgccg acgacgcccg tggtcagatc ggccttggcc
     3661 agccaggccg cgcgcttggc cttttcaaaa tcgacccgca acagttcggt caacaccatc
     3721 gccacgaagg tgacgcgatc gacgcgatcc tgcatcgtgc ccaccttggc gtggaacacg
     3781 atgtccttga gcgcgttgac tcgcttttcc agccggatct tgcggtcctg gtcccagaag
     3841 aatttcgcgt ccgacaggcg cgcgcgcagc acgcgctcgt tgccggcgac gatctctttg
     3901 ccgccgtcgg ccgagaccat gttggaaacg accgcgaagc ggttcgccat ccgcccggtc
     3961 ttggcgtcgc gcagcgagaa atacttttgg tgcgtgcgca tggaggtctg caaaatctcc
     4021 ggcggcaggt ccatgaactg gtcctcgatg gtgccgatca gcaccaccgg ccattccgcc
     4081 agtcccgcaa cttcatccag caggccctcg tccgcgatca gttcgagacc cagcgcgaac
     4141 gccttctgcc ccgcttcgtg caggatggtc tctttgcgct cggcggcatc gagaacaacg
     4201 tgcgcctcgc gcagcttccg gacgtaatcg tcgaagcggc gcgcttcgat ttcgccttgc
     4261 gacaggaagc gatggccccg cgtcgcgcgc ccgcttttca cgccggcgaa ttcaaacggc
     4321 acggtctcgc cgtcgaaggt gcaaaggatg gaatgcagcg gccgcaccca gcgcaccgga
     4381 tcgcccggaa gccagcgcat cgatttcggc caaggcagct tggcgacggc ttcgggcaca
     4441 agctccgcca gaacctcggc cgtctcgcgg cccttgcggg cgatcaccgc gacatagaat
     4501 tcgcccttgc cgtcgctccg cttctcgcat tgttccagcg tcaccccggc ggcgcgcaag
     4561 aagccctcca acgccttttc gggcgcgccg acgcgcggcc ccttcttttc ctcgctgaca
     4621 tcgggctgct tgacgggaag cccggccacc gccagcacca gccgccgcgg ccccgcgaac
     4681 gccttggcgc cttcgaacag cagtccgcgg tccgacaggc cgccgacgat cagacgctcc
     4741 agatccttgg cggcctgggt ctgcatccgc gccgggattt cttcgctgaa cagttccaaa
     4801 agcagatcgg gcatctcagc catacctcgg cttgtattcg cccatcggcg acgccagcca
     4861 gctttcgcag caggccttgg ccagcgcccg cacccggccg atataggcgg cgcgttcggt
     4921 caccgagatc acgccgcgcg cgtccagcag attgaaacag tggctggcct tgatgcattg
     4981 gtcgtaggcc ggcagcgcca gcttggcctc caccagcgcc tggcattcct tttccgcatc
     5041 ctcgaaatgg cggaacagga tcgcggtgtc ggcgcgctcg aaattgaagg ccgagaattc
     5101 gcgctcgctc tggtgaaata cttcgccgta gcgaaagttt tcgttgaaag cgaggtcgta
     5161 gacgaactcc acgccctgca catacatcgc cagccgctcc aggccgtagg tgatctcggc
     5221 gcagaccgga tcgcaatcga tgccgccgac ctgctggaaa taggtgaact gggtgatctc
     5281 cattccgtcg caccagactt cccagcccag gccccaggcg cccagcgtcg ggctttccca
     5341 atcgtcctcg acgaagcgga tgtcgtgcag cgccgggtcg aggccgatgg ctttgatcga
     5401 gccgagatac agttcctgca cgtcctcggg cgccggcttc aggatcacct gatactgata
     5461 ataatgctgg agccggttgg ggttctcgcc gtaacgcccg tccttgggcc ggcgcgaagg
     5521 ctggacatag gcggcgcgcc acggcctggg gccgagcgcc cgcaaggtgg tcgccgggtg
     5581 gaaagtcccc gcacccattt ccacatcata gggttgcagg ataagacagc cctgctctgc
     5641 ccagtagttc tgtagcgtca ggatcaggtc ctgaaaggtc ttggccacga ttcggctttc
     5701 gtcaaacggc gggcggaacc tacttgcccc ctcatgcttc gacaagccag catgaagcta
     5761 tctcagccac cctcaccctg agctggccga agggtgaggg ccgccccatc cctcagcacc
     5821 gcatccgctt gcggcgtgta gcggccgtcg gcggcgtgca gcaccaggcc gggcaggatc
     5881 gccagcggcg cacggctgcc ctggcggacc tggacgatga cccgctttgc cggggcattg
     5941 gcgcggggcc agagcgggaa gaccgtcacg ccgcgttcgg gcagcgcagc cagggcttcg
     6001 cccagccggt cggcgcgcaa gatggcagtg aatgtcccgc ccgcgatcac gcgcttgaac
     6061 cccgtaagcc aatcggtcag ccgcccgccg tcctgcagcg ccgccgcccg ggccgcatcg
     6121 ggcgaggacg caccctctcc gtgaaatggc ggattgcaga acacatgccc gaagggtctg
     6181 cgcaacggcc ccggcggctg gagcgcgtca cccgctgcga acgtcgcgca tacactcatc
     6241 ccgttcgcgg aagagttggc cgtcgccaat tccaccagcg cgggatcgat ctcaattcca
     6301 gtgacggaga tgaggcccac gcggtgcacc aggcacaggc tggccgtgcc ggccccggcg
     6361 cccagttcca aagcggtttc gccaggacgc gccggcaccg ccgccgccag catcaccgca
     6421 tccagtccgg cgcgaaagcc gtccaccggc tggcgcacca gcacgcgccc gcccaggaac
     6481 cggtcctcgg ttgttgcgat cacaatttcg tgtctctgaa attagacaag gacgtcatcc
     6541 tttacggctc gaccggcgca tccttgatgc cgtcgcgtaa gatagcctgg gcgcggacga
     6601 aatcctcgtc cgcgaccatc agccggcgcg gcagcatccc gatgctgccg tccatgacgc
     6661 tcgcctcgcc gtcgaacacg acggactgca gcccggcatc cgccagtaga acctccgcga
     6721 agttcaacag cacgggatcg ttggttttca ggacggcgcg catgcgttct cccagcctta
     6781 ggattggatt accatagcac tgtccgcttg accgcgcttt accggccttc caatctccca
     6841 aacgccgggt gtatccggca agcccgtctg gtatggttcc ctatgcttgc atccgaagcc
     6901 cgcaggtcga atgccacgaa agatgccggt gtttcgccgg tcgagcgcct ccacgccctg
     6961 gtatcggccg acatgcgcgc gaccgacgcg ctgatccacg aacgggcggg cagcaccgtc
     7021 gcccttattc ccgatctggc acgccatctg atcgattccg gcggcaagcg cctgcgccca
     7081 ttattgacgt tggcggccgc ccggatgggc ggctatgccg gtcacgacca tgtccggctc
     7141 gccgccgccg tcgagttcat ccataccgcc accttgctgc acgacgacgt ggtggacgaa
     7201 agcgcgctgc gccggggcaa ggtctcggcc aacatcgtct ggggcaacaa gcccagcgtg
     7261 ctggtcggcg actatcttct cagccgcgcc tttcagttga tggtggagac cggcgagttg
     7321 gcggtcctcg acatcctggc cggagcgtcc gcgatcatcg ccgatggcga agtgatgcaa
     7381 ctcaaatcga cgaacaacct cgcggccacc gagcagcatt atttggcggt cgtccgcgcc
     7441 aagaccgcgg cgctgttcgc cgccgccgcc gaatccggcg cggtgctggc gaagcgctcg
     7501 acggattacg tcgcggcgct gcgttcctat ggcgataatc tcggcatcgc tttccagctt
     7561 gtcgacgacg cactcgatta ttccggccgc caggcgctga tgggcaaatc cgtcggcgac
     7621 gatttccgcg aaggcaaggt gacgctgccg gtgatcctcg ctcatgcccg cgccgacgaa
     7681 gaggggcgcc gcttctggcg gcgcgccatc gagaccggcc cgcaaggcga aagcgacctc
     7741 gaccgcgcca tcactttggt ggaggagacc ggcgcgattt ccgaaaccat ggcgcgggcg
     7801 cgcgcctatg ccgaccgggc acgccaagcc ctggccgcgg tcgcgccttg cgaaacccgc
     7861 gaggtgctgg ccgacatcgc cgatttctgc gtcgagcggg cgtattaggc cgcaactatc
     7921 caccaattgc catgtcgcgc gcgaatggcc gcagccgcgt tctgcgccga agcaccgctg
     7981 tcaaacagcg cgaagcacgt cgcgccgctg cccgacatgc gcgcgagcaa gaggcccggc
     8041 tgcgacgcaa gcgcgtccag cacttcgccg atgtgcggcg cgatctggcg cgcgggggct
     8101 tccagatcat tggcggtggt tttcaggtaa gcgacaagtt catccgcgtt cttcatcgcg
     8161 tccggaagcg gcaggccgac gccccggcgt tccttcaacg cggcgaacac cttgccggtc
     8221 gggaccgcga cgccggggtt gaccagcacc atcggaactt cgacaattcc cgccagcgcc
     8281 gtcaccttct cgccgcggcc ttccatccag gcgggcttgc ttccgacaca taccggaacg
     8341 tccgagccga tttgcgcggc gatagcctga agctcttccg gcgcgagatt gagtttccag
     8401 agcgcgttca gcccgcgcaa tgtcgccgcc gcgtccgccg aaccgccgcc gatgccggag
     8461 gcgataggca ggtttttcgt cagcgcgatg cgcgcgcctt tgcggacgcc agcgctggcc
     8521 gccagcgcgc gcgcggcctt cagcacgaga ttgtcgggac cgggcgtcag cgcattcgcg
     8581 aaaggcccgt ccagcgcgag cgagagttcg ccggcgcctt cgaaagccag ttcgtcaccc
     8641 gtgcatgcga aaacgaccag gctttccagc gcatgaaacc cgtcggcgcg tttctgcccg
     8701 acatgcagga acaggtttat cttggcaggc gcgaagacat gcaaggcatc agcttcggtc
     8761 gtcacggggc agcccggcct ccagcttctt ctcgattttc tctttgtcct tgccctccgc
     8821 gccgaaggcg agcgcgtggt tccattgaaa ctgcgcgtcg agcttgcggc cgactttcca
     8881 ataggcatcg cccagatggt cgttgatggt gggatcgccg ggtacgatgc gaacggcttt
     8941 ttccagcgcc ttgacggcat cggcgtagcg gccgagatgg taataggccc agcctacgct
     9001 gtcggtgatg taaccgtcgt ccggcgccaa ggcgctggct ttctccagca tggccacggc
     9061 ctgggaaatg ttgcggcgtt gatccaccca gctatagccg agataattca gcgtctgcgg
     9121 ctcgtccgga ctgagcatga gcgccatttc gagatcggcc tccgccgctt tccagtcgtt
     9181 cgattgctgt gcggcgatgg cgcgggcgta ataaagcggc cacgacttct tcgtcggctc
     9241 gccggcggca aggatcgcac ggtcataggc cttggtggcg tcggcatatc tttccgcctg
     9301 gcgataggca tcgccgagcg ccgtccaggg ttcgacctcg ccgggatcgg ccgccgtcaa
     9361 cttcgtcagg ccggcaatgg cttcgtccat ttttccggag cgcaccagat caaccgcgat
     9421 ctgcaccgcc gcgatgcggt aataggggga ctccgtgccg acgccgcgaa gaatctcaac
     9481 ggcgttgtcg tacttcgtca agccttcgaa acggtccgcc agcaacagct tggcgagatc
     9541 gagattgggc cgcagataaa gcgccaggcg caggtaaagg atggaaacgt cccggctgga
     9601 tgcgtcgccg agcgaagcgg cgatcccaaa cagggcctcg gccgcaccgt cttcggcatg
     9661 cacgatcagc gcatcgggcg tccggcctga tgcgatgcgc gccaagcccg cattggcgac
     9721 aggcgtaaag ctgccttcgc cggctatttt ctcgtagagc gctttggctt ccggcacgcg
     9781 cttgttgcgc tccaggaagc ggccgtaagc gtcgatgaca cgcggcgaca gtccgccggc
     9841 cttcatttcg agattgtaga acgcatcggc tttgtctttc tggccgaaga actccgccag
     9901 catcgcttcg ttgaaatagg tgagcgcatc cgcgccgctc tgcgcatgca ggcccttcag
     9961 ttccgcttcg gccgctgcca tgtcgcccat cccggcagcg gcccaggcgt cgatcagtgc
    10021 gaccgtgaac agggtgaa
//
LOCUS       DASWPU010000365         4198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_70824,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000365 DASWPU010000000
VERSION     DASWPU010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70824"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..2269
                     /locus_tag="VGC27_07920"
     CDS             131..2269
                     /locus_tag="VGC27_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HEY0282533.1"
                     /translation="MQINPALSTLPRRLGVALFRSHIENGLAVSLGMAVVGLGTALFF
                     GRDIAILAATGALCASVVDQPGPYAIKTRIFALAVGGATALTSLTILALDSPLLMGPL
                     VAAMSFVTGMISAYGRRAIGLGIAAVLALMFGMMIPGSIPLLWHTAVFAGGGIAYACF
                     ALLMGAILDDRNRRQYLGEAVRAFGAYVAAKAELYNPQTRPRLAEQALIEAHATFVER
                     LQSARDIIFFGKQTPNRVRWMAALILLLDCFDFVLSSDADIETLRQSAHRDLLRRLGA
                     LTAGIAENMQELALALATPGATFAYTGHESPLQEIGDEVHHLEGAAEGGGEPLELSAF
                     RSTWHKLTQTITRLGRLTEVINASDPAEAKLPNVDLSLFRQIESMNPRLLLAQMRLSS
                     PVMRYAIRLALAMSCGYAVTLLLPNMLHGGWVLLTTMLIMRASYSITKQRRNDRIVGT
                     VLGCVVAAILVHTLPRDWLFAPIIVTVGLAHAFAAVDFRITALSASITALLSLHFLAP
                     ETGGIFFERILDTLIGAGLAWSFSFLLPSWEWRNIARLVAAKVSADRKWAAVALARQR
                     DDQTYRLARKQAHDATANLSMTVRRLVDEPQVDRRALVALNDLLGANYLFASDLASMR
                     VLFRMRAGELEPAVADALLETARTNVAMSLAAPGAKDAPVPRLSRRSLRENLGGANAM
                     ISLRRRLVHIERTAERVAALAARATSQVSD"
     gene            complement(2420..2956)
                     /locus_tag="VGC27_07925"
     CDS             complement(2420..2956)
                     /locus_tag="VGC27_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0282534.1"
                     /translation="MTNIADIPVLETARLRLRAVRIEDYEDLVAIWSDTSVARYFGDQ
                     TLSREDIWRRLMGHAGQWALLDNGSWAVEEKDTGAFVGSTGFVNAKRDIEPSLEGMPE
                     MGWALSPSARGKGYATEAARAALAWGDVRFGKTTMVCIIHPENRASIRVAEKIGFRFW
                     RNGIYRNEPTAIFTRETV"
     gene            complement(3022..3375)
                     /locus_tag="VGC27_07930"
     CDS             complement(3022..3375)
                     /locus_tag="VGC27_07930"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEY0282535.1"
                     /translation="MKRTNSFGRGSTDIETRLWDKLRAFDELGYQFRRKAHFKSFVLD
                     FVEHDALLVVELDDGAPGRAEPRNAARDHVLHEAGYTVLRFWKSEAAADLNGVIAAIR
                     QVLEDRPPLSSNIPK"
     gene            complement(3379..>4198)
                     /locus_tag="VGC27_07935"
     CDS             complement(3379..>4198)
                     /locus_tag="VGC27_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY0282536.1"
                     /translation="RRLYDFLPLNNREKPPHWPTEDEPQRVEHSLDTLVPENPNKPYD
                     MRELILKVVDEGDFFEIGEDFARNIITGFGRLEGSTVGFVANQPMVLAGVLDSDASRK
                     GARFVRFCDCFNIPIVTFADVPDFLPGTAQEHGGIIKHGAKLLFAFTEATVPKVTVIT
                     RKAYGGAYIVMASKHLRADVNYAWPWAQIAVMGAKGAVEIIHRKDAGNPEAIEAHTKE
                     YEEKFLNPFVAASRGWIDEVIRPHNTRKRIVGALRMLRNKQVPQVWKKHDNIPL"
BASE COUNT          706 a         1387 c         1295 g          810 t
ORIGIN      
        1 caggcgcgaa tcacgcgaac ggcgatttcg ccgcgattgg caatcaggat cttcttgaac
       61 acgctttcac cgggcaggaa cggcggcttt tgcagttgct tttaggaggt cccggcgttc
      121 aggtaaaccc atgcaaatca accccgctct gtccaccctt ccgcgccgcc tgggcgtcgc
      181 cttgttccgt agccatatcg agaacggctt ggccgtttcc ctgggaatgg ccgtggtagg
      241 gctgggcacg gccctgttct tcggccgcga catcgccatc ctggccgcaa ccggggcctt
      301 gtgcgccagc gtggtcgatc agccgggccc ctacgcgatc aagacacgga tttttgccct
      361 ggcggtcggg ggagccacgg cgctgacctc tctgaccatc ctcgccctgg attcaccctt
      421 gctgatggga ccgctggtcg cggccatgag cttcgtcacg gggatgatct ccgcttacgg
      481 gcggcgcgcc atcggcctgg gtatcgcggc ggtcctggcg ctgatgttcg gcatgatgat
      541 acccggctcc atacccctgt tatggcatac ggcggtcttc gccgggggcg ggatcgccta
      601 tgcctgcttc gccctgctga tgggcgccat tctcgacgac cgcaaccgca gacagtacct
      661 cggcgaagcc gtgcgggcgt tcggcgctta cgtcgcggcg aaggccgaac tctacaatcc
      721 gcagacgcgc ccgcgcctgg cggagcaggc ccttatcgaa gcccatgcga cgttcgtcga
      781 gcggctgcag tcggcgcgcg atatcatctt cttcggcaag cagacgccga accgcgtccg
      841 ctggatggcc gctttgatcc tgctgctcga ctgtttcgac ttcgtcctgt cgagcgacgc
      901 cgatatcgag acgctgcgcc agtccgccca tcgggatctg ctgcgccgcc ttggcgcctt
      961 gaccgccggc atcgccgaga acatgcagga attggcgctg gcgctggcga cgcccggcgc
     1021 cacctttgcc tataccgggc acgaaagtcc gttgcaggag atcggcgacg aggttcatca
     1081 cctggaaggt gcggctgagg gcggcggcga acctttggaa ctgtccgcgt tccgttccac
     1141 ttggcacaag ctcacgcaga cgattacgcg cctcgggcgg ctgaccgaag tgatcaatgc
     1201 ctcggacccc gcggaagcga aacttcccaa tgtcgatctc tccctcttcc gccagatcga
     1261 gagcatgaac cccaggctgc tgctggcgca gatgaggctg tcctcgccgg tgatgcgcta
     1321 cgccatccgc ctggcgctgg cgatgagctg cggctacgcc gtcacgctcc tgttgccgaa
     1381 catgctgcac ggcggctggg tgctgctgac gaccatgctg atcatgcgcg ccagctacag
     1441 catcaccaag cagcgccgca acgaccgcat cgtcggtacg gtgctcggct gcgtggtcgc
     1501 ggcgatcctg gtgcatacgc tgccgcgcga ctggctgttc gcgccgataa tcgtcacggt
     1561 cggcttggcc cacgccttcg ccgccgtcga tttccgcatc accgctttgt cggcatcgat
     1621 cacggccttg ctgtcgctcc attttctcgc gcccgagacc ggagggatat tcttcgaacg
     1681 catcctcgac acgctgatcg gcgccggcct cgcctggtcg ttcagcttcc tgctgccgag
     1741 ttgggaatgg cgcaatatcg caaggctggt ggccgcgaag gtgagcgccg accgcaaatg
     1801 ggccgccgtg gcgctggcac gccagcgcga cgaccaaacc tatcgcctgg cgcgcaaaca
     1861 ggcgcacgac gccaccgcca acctgtcgat gaccgtgcgc cggttggtcg acgaaccgca
     1921 ggtggaccgg cgcgccctgg tcgcgctgaa cgaccttttg ggcgcgaatt acctgttcgc
     1981 ttccgacctt gcctccatgc gcgtgctgtt tcgcatgcgg gcgggcgaac tggagcccgc
     2041 ggtggccgac gccttgctgg aaacggcgcg gaccaacgtg gccatgtcgc tcgcggcgcc
     2101 cggcgccaag gacgctccgg tcccgcgcct gtcgcgccgc agcctgaggg aaaacctggg
     2161 cggcgccaac gcgatgatct cgctgcgccg ccggctggtt catatcgaac gcaccgcgga
     2221 acgcgtggcg gctctggccg cacgcgcaac ctcgcaagtt tcggactagt gcgtgatcca
     2281 tccacatggg atcgtgccac acccatgctt cgacggtcgg tagcaacagc gtgccgacca
     2341 gcatgaggga atagcaaaaa ccctaaccct gagcttgtcg aagggtgatt gaagcgattc
     2401 agctaggatg gatcacgttc tacacggttt cgcgcgtgaa gattgccgtc ggctcgttcc
     2461 tgtagatccc gttccgccag aagcgaaagc cgatcttctc ggccacgcgg atggaagcgc
     2521 gattctccgg gtggatgatg cagaccattg ttgtcttgcc gaagcggacg tcgccccagg
     2581 ccagcgcggc gcgcgccgct tccgtcgcgt atcccttgcc gcgcgcgctt ggcgaaagcg
     2641 cccagcccat ttcaggcatt ccctccagag agggttcgat atcgcgcttc gcattgacaa
     2701 agccggtgct gccgacgaaa gcgcccgtgt ccttctcttc gaccgcccac gaaccgttat
     2761 ccagcagcgc ccactgtccg gcatggccca taagccgccg ccagatgtct tcccggctca
     2821 aggtctgatc gccgaaatac cgggccacac tggtgtcgga ccagatcgcc accagatctt
     2881 cgtaatcctc gatacgaacg gcgcgcagcc tgagccgcgc agtctctaga accggaatgt
     2941 cagctatgtt ggttatggga cgctcccctg tgcttgccgc ccgtgaggta cctctcccat
     3001 gcgccgggcg ctatgaaaca attatttcgg aatgttggag gaaagaggcg gccggtcctc
     3061 aagcacctgc cggattgccg cgatgacgcc gttcaaatcc gccgccgctt cgctcttcca
     3121 gaaccgcaga acggtgtatc cggcttcgtg cagcacatga tcgcgcgcgg cgttgcgcgg
     3181 ttccgccctg cccggcgcgc catcgtcaag ctcgacgacc agcagcgcgt cgtgctcgac
     3241 aaaatccaaa acgaacgatt tgaagtgcgc tttgcggcgg aactgataac caagctcatc
     3301 gaacgcgcgc agcttgtccc atagccgcgt ctcaatgtcc gtcgaacccc ggccaaagga
     3361 atttgttcgt ttcatctctc aaagcggaat attgtcgtgc ttcttccaca cctgcggcac
     3421 ttgcttgttc cgcagcatgc gtagcgcgcc gacgatgcgc ttgcgggtgt tgtgcgggcg
     3481 gatcacttcg tcgatccagc cgcgcgacgc cgccacgaac gggttgagga acttctcctc
     3541 atattccttg gtgtgggcct cgatagcttc ggggttgccg gcgtccttgc ggtggatgat
     3601 ctcgactgcg cccttggcgc ccatcaccgc gatctgcgcc cacggccagg catagttcac
     3661 atcggcgcgc aaatgcttgg aggccatcac gatataggcg ccgccataag ccttgcgggt
     3721 gatcaccgtc accttgggca ccgtagcctc ggtaaaggca aacagcagct tggcgccgtg
     3781 cttgatgatg ccgccatgct cctgcgcggt gccgggcagg aagtcgggca cgtcggcgaa
     3841 ggtcacgatg ggaatattga agcaatcgca gaagcgcacg aagcgcgcgc ccttccggct
     3901 ggcgtcgctg tcgagcacgc ccgccagcac catcggctgg ttggcgacga atccaaccgt
     3961 ggagccttcc aggcggccga agccggtgat gatgttgcgc gcgaagtcct cgccgatctc
     4021 aaagaaatcg ccctcgtcca cgaccttcag gatcagttca cgcatatcgt agggcttgtt
     4081 ggggttctcc ggcaccagcg tatcgagcga gtgctcaacg cgctgcggct cgtcttcggt
     4141 cggccagtgc ggcggctttt cgcggttgtt gagcggcagg aaatcgtaga gccggcgg
//
LOCUS       DASWPU010000366         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_94961,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000366 DASWPU010000000
VERSION     DASWPU010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94961"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VGC27_07940"
     CDS             complement(<1..696)
                     /locus_tag="VGC27_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282537.1"
                     /translation="MGTYNLTAEQFNKLQDYADFSNRLSKEDKASGKTLADLLAEDKA
                     AASALAAKMLPSCQVNEAILAAQGAVTVNGKPVNTKTYEAACANGMGYFLVSQDVGDP
                     YAISCFAADATRAADVAAGRTPGAVCQLPSNIDVNAMATSLLNHAGTNCTVSDHRWVG
                     QSSATKTEYNEVVCTDHSGYMLAIALPGSNATVRVSTCHDAAMRGLPCKLSAGGGAVI
                     TLQTFRDALEQHKI"
     gene            1381..>3629
                     /locus_tag="VGC27_07945"
     CDS             1381..>3629
                     /locus_tag="VGC27_07945"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0282538.1"
                     /translation="MNPTADKLSGSVKALLLGTTMLAGVGTALLAVTPAIAQEEAVET
                     VVVTGIRASLQSAQTIKQNSDQIVDSITAVDIGALPDSSVAEALQRVPGIQISRTDQP
                     NDPLRWAGYGNGVFIRGLSWVKSLTNGEEIFGAENGRTISFADISPDLMSGVDVYKNP
                     SAKMIEGGVGGTVDLKTRLPLDFDGRKVAASGTFDYGTLSDQAGLSGNVLISDRISTK
                     VGEIGALFSASYQNLVSGNNIGTTDPWTNLQRTGPWTNGTDNYPPFRTDEYYPRGLTK
                     AFGMVGYRHMDWKQPRVSFDATLQWRPNDALEITLVGLYTRAEPQSVEHNVAWVVPVV
                     NTQYCGLHMDQSEALEGDGSCTYPIGSLSTLQAADMASANASMASYTYDANGYLNSGT
                     IFNAQSDSTYVNYFDTRYDVRHHTNKNLELTVKWQPNNNLSVALDTSYIDSRATMSSL
                     TMYYGTKSDRYMQSSNYSGAAEFYPNAPHIDVGFDFTNASPNFTYNDAGIAALADQSS
                     YLWAAFMDHYENNYAHAYTSRADATYTFEGNGLFGWIRSAEAGIRGAFKQSATRNTGW
                     NWGRVGFATWNTLNCGRRSDYGGNFRDPLECAAEVGDFSTWMADRSEFYDFPTYFGQK
                     LPGIWAPTVNWMKHPYQVWSDLQPMLTGLEQLQWPYNSDGTLADDATNAAAGTWIPAT
                     LRDGACTGVPYLCENVYISGTNNIQREDTLAAYFMLNFAHRTFLGLDVPVDGNIGVRV
                     VNTQYNSGPG"
BASE COUNT          786 a         1120 c         1023 g          700 t
ORIGIN      
        1 gatcttgtgt tgttcgaggg cgtctctgaa ggtctgaagc gttatgaccg cgccgccgcc
       61 ggcggacagt ttacagggca ggccccgcat cgccgcgtcg tggcaggtcg aaacccgaac
      121 cgtcgcgttg gaacccggca aggctatggc cagcatgtag cccgaatggt cggtgcagac
      181 gacctcgtta tactcggtct tggtcgccga gctctggccg acccagcggt gatcgctgac
      241 cgtgcagttc gttccggcgt ggtttagcag cgatgtcgcc atcgcattca cgtctatgtt
      301 ggacggcaac tgacacaccg cgcccggcgt cctgcctgcc gctacatccg ccgcgcgcgt
      361 tgcatcggcg gcaaaacaag aaatcgcgta agggtcgccc acgtcctgcg aaaccaggaa
      421 atagcccatg ccgttggcgc acgcggcctc atatgttttg gtgttgacgg gtttgccgtt
      481 caccgtgacc gccccttggg cggccaggat ggcctcgttc acctggcagg acgggagcat
      541 cttcgccgcc aaagccgatg cggcggcctt atcctcggca agcaggtcgg caagggtctt
      601 gccgcttgct ttgtcctctt tcgacagccg gttcgagaag tcggcataat cttggagctt
      661 gttgaactgc tccgccgtca ggttgtaagt gcccatctgg gcgcccggag ccaccggtat
      721 tccagggccg ctggatccgc cggacggtcc ccccgcgttt tgcgccgaag ctgaatttgc
      781 agacacgcta gcggccaagc aggccgccgc aatcgtcata atgccaaacc tcatttcgcg
      841 tataccccct gtattttgag catggccccc agccgcccac accgcagcat agccctagcg
      901 atacggacat caccttggaa taaccgtaat caggtccgaa atactaaact tgacgaggcg
      961 aatgtatcaa tcagactcta agtgtcatcg gaaaagcacg tgacgtttag tcatattata
     1021 taggtatatt ataaccaaac taattcgtac ttacggccag ttggcatcct tactatattg
     1081 ttatgaaagc aagattttca ctaaatgcat attttaatca tttaggcatg cattcgacag
     1141 acacaatagc aacacacgat agaaatgtcc ggcgctgtct ttagaattcg ttgaaacccc
     1201 cacgagaatg tggcgtaatt gtgaagatga tagcgttatc atcaacggac cggggggaac
     1261 tgctgcaatc aaaaaagtaa gcgctaacgt gctggcagca actcttcgtg tgcggcgaag
     1321 aggccgacaa aaaatggcgg cttcaaaggc ccaggaaaca tcttagggga gaaacgaata
     1381 atgaatccaa cagcggacaa attgagcggt agcgtcaagg cccttttgct gggcacgacg
     1441 atgcttgctg gggtcggtac ggcattactt gctgtgacgc cggccatcgc gcaggaggag
     1501 gcagtggaaa cggtggtcgt caccggcatc cgcgccagcc tgcaatccgc ccaaacgatc
     1561 aaacagaact ccgaccagat tgtagactcc attaccgccg tcgacatcgg cgccctgccg
     1621 gacagcagcg ttgcggaagc cttgcagcgc gttccgggca ttcagatctc gcgcaccgac
     1681 cagcctaacg acccgctgcg ttgggccggt tacggcaacg gcgtgttcat ccgcggcctg
     1741 tcctgggtca aatcgttgac caacggcgaa gaaatcttcg gcgccgaaaa cggacgcacc
     1801 atcagcttcg ccgacatctc gccggacctg atgtcgggcg tggacgtcta caagaaccca
     1861 agcgccaaga tgatcgaagg cggcgtcggc ggcaccgtgg acctcaagac ccgcttgccg
     1921 ttggatttcg acggccgcaa ggtggcggcg tcgggcacgt tcgattacgg cacgctctcc
     1981 gatcaggccg gtctgtcggg caacgtcctg atcagcgacc ggataagcac caaagtgggc
     2041 gaaatcggcg cgctgttttc ggccagctat cagaacttgg tgagcggcaa caacatcggc
     2101 acgaccgatc cttggacgaa tttgcaaagg accggcccgt ggacaaacgg caccgacaac
     2161 tatcctccgt tccggacgga tgagtactat ccgagaggcc tcaccaaggc cttcggcatg
     2221 gtcggctatc gccacatgga ctggaagcag ccgcgcgtat ccttcgatgc cacgctgcag
     2281 tggcggccga atgacgccct cgaaatcacc ttggtcggct tgtacaccag ggccgagccg
     2341 cagagcgtcg agcacaacgt ggcctgggtg gttccggtgg tcaacactca gtattgcggc
     2401 ttgcatatgg atcaaagcga ggcgctggaa ggcgatggca gctgcacgta ccccatcggt
     2461 tcgctgtcga ccctgcaggc cgccgatatg gcttcggcaa acgcttcgat ggcgagctac
     2521 acgtatgacg ccaacggcta cctgaacagc ggcacgatct tcaacgccca gtccgactcg
     2581 acctacgtca actacttcga cacccgctac gacgttcgcc accacaccaa caagaacctc
     2641 gaactgacgg tcaagtggca gccgaacaac aacctttcgg tggctttgga cacgagctac
     2701 atcgattcgc gcgcgaccat gtcgagcctg acgatgtatt acgggacgaa gagcgaccgc
     2761 tacatgcagt cgtccaacta ttcaggcgcc gccgaattct atccgaacgc gccgcatatc
     2821 gacgtcgggt tcgatttcac caacgcttcg cccaacttca cctacaacga cgccggcatt
     2881 gcggcactcg ccgatcagag ctcatatctc tgggcggcct tcatggacca ctacgaaaac
     2941 aactacgcgc acgcctacac gtcccgcgcc gacgccacct acaccttcga gggcaacggc
     3001 ctgttcggct ggatacgttc cgctgaggcc ggtatccgcg gcgcgttcaa gcagtcggcc
     3061 acgcgcaaca ccggttggaa ctggggccgc gtcggtttcg cgacttggaa caccttgaac
     3121 tgcggccgtc gctccgacta cggcggcaac ttccgtgatc cgctggaatg cgccgccgaa
     3181 gtgggcgact tctctacctg gatggcagac aggtcggagt tctacgactt cccgacctac
     3241 ttcggtcaga agttaccggg catttgggct ccgaccgtga actggatgaa gcatccttac
     3301 caggtctggt ccgatcttca gccgatgctg accggcctgg agcaactgca atggccgtac
     3361 aactccgacg gcactctggc cgacgacgcc acgaacgcgg ctgccggcac gtggatcccg
     3421 gccaccctgc gcgacggcgc atgcacgggc gtgccctatc tgtgcgaaaa cgtctacatc
     3481 agcggcacca acaacatcca gcgtgaagac accttggccg cctatttcat gctcaacttc
     3541 gcgcatagaa ccttcctcgg cttggacgtc ccggtcgacg gcaacatcgg cgtccgtgtc
     3601 gtcaatacgc agtataacag cggcccggg
//
LOCUS       DASWPU010000367         4902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_2871, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000367 DASWPU010000000
VERSION     DASWPU010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2871"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VGC27_07950"
     CDS             <1..560
                     /locus_tag="VGC27_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0282539.1"
                     /translation="PLLQIVTAVPVFCAGLALAYGAVHLLHWPVSVNAAAALSTPIYS
                     LNPQDLEFAALPVLMVGLAGAAAVQLALRKAIAESAGKSFRTGLKRMGLSALEIERVY
                     TVPRVIAGLLASAGEIVLALLSAAVVAEWVFHRAGAADLFVKSVALHDWNMTALILFV
                     FAGLAFLAEILGRVSAYALANEGKT"
     gene            557..1375
                     /locus_tag="VGC27_07955"
     CDS             557..1375
                     /locus_tag="VGC27_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282540.1"
                     /translation="MSALETQAAPILRAVLRLIGWGGLGLIAVAVALSDFLASAPAGT
                     VGLGPILAAPSPDHLFGTDLLGRDMLSETLHGLSVTAGHALVAALVAITMGGLFGAVA
                     ARLPRLFGLSLRGAIGVFAAIPALLLAILAIGLSTRFYAPLAAGLAAAPLAFIRAYDR
                     VEAQSDSTHAGYARATGIAAVTLLRRDLTYEFRGTVAAAAARALAAVTIILSTVSFLG
                     FGATPPARDLGLMIAAAKASFLHAWWAAAFPALALIVLILCARMAAGLDEGERP"
     gene            1372..2949
                     /locus_tag="VGC27_07960"
     CDS             1372..2949
                     /locus_tag="VGC27_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0282541.1"
                     /translation="MSEPAAILDVAELTVARPGAAPELDRFSLVLEAGESAIVLGEDG
                     SGKEALIRVLGGFAERTDQISGTLRFGNAEARPAAKRARAPIRIAYLPNPLAQPFAAH
                     AGVLAQLTRVVARKLGSPLTSAREELRLALERLDGAPLLALLNEKPQVLDPVSTAWGL
                     LACACAQTPDLVLADHAFADLGPTATQALLSALATARKRLGFALLYATGSLQPAARLG
                     GRVVVIRHGRMVEEGDVTRLASGHAHAYTNTLFKALPRLSLDGQKSRNAARGQPLLQV
                     HGLDLGALGGREAITFELRRGASLALVGEEGSGRRALVRAVLGLDRAPAGRVVFDAVD
                     LNILSQTMTARLRRRVAFITGADDVLDPRMSLWDAVDEPLRAHLKLTGELVAGYRETA
                     LKRVGLASHDGSRTVATLGAFDKRRLQVARAIVSAPLLAVVDEPLRGLDAFAQTIMRE
                     VLTDFRTQQDPAFLVITSDFTVAQALADEVMVFKDGKIVERGPIAAVLNAPKDPCTKS
                     LIEAVSLPGLSQTPPSV"
     gene            3079..4383
                     /gene="purB"
                     /locus_tag="VGC27_07965"
     CDS             3079..4383
                     /gene="purB"
                     /locus_tag="VGC27_07965"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY0282542.1"
                     /translation="MIPRYARAEMAAIWSAETRFRIWFEIEAHAADAMAKLGVIPKEA
                     AAKIWEKGKAAKFDIGRIDEIEREVKHDVIAFLTHLAEIVGPEARFLHQGMTSSDVLD
                     TCLAVQLRRAADILIADVDLLLAALKTRAFEHKYTPTIGRSHGIHAEPTTFGLKLAGY
                     YAEFLRAKARLLTAREEISTCAISGAVGTFANIDPPVEAYVAGKLGLKPEPVSTQVIP
                     RDRHAAYFAALGVAAASLERLATEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVL
                     SENVTGLARMVRAYVVPALEDVALWHERDISHSSVERYIGPDATITLDFALARMAGIV
                     EKLIIYPKRMQENLDKTRGLVHSQRVLLALTQKGLGREESYRLVQKNAMRAWNGEGAL
                     LDLLKADEDVSRALSAAELENLFDFGYHFKQVDTIFARVFAE"
     gene            4391..4690
                     /locus_tag="VGC27_07970"
     CDS             4391..4690
                     /locus_tag="VGC27_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282543.1"
                     /translation="MRIVELDAGGFKTPLDFLAAVRRAIGAPQEPGLNIDTLVGAMLR
                     GCSAGPCVLRIVGMGKAPSPVREEAEALVKALKDASLWRHNHRYEDIGVAIEIVP"
     gene            complement(4703..>4902)
                     /locus_tag="VGC27_07975"
     CDS             complement(4703..>4902)
                     /locus_tag="VGC27_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282544.1"
                     /translation="LPVSFRFRNSPETGRGLALLQAQARLRARAARTIDALWDAIGDI
                     CDLYSPTECSNYFAASGYYPN"
BASE COUNT          772 a         1643 c         1612 g          875 t
ORIGIN      
        1 cgcctttgct ccaaatcgtg accgccgtgc cggtattctg cgccggcctg gcgctggcct
       61 atggggcggt gcatctgctg cattggccgg tcagcgtcaa tgcggcggcg gcgctctcca
      121 cgccgatcta ttccctcaac ccgcaagacc tggaattcgc cgccttaccg gttctgatgg
      181 tgggacttgc cggagccgcc gccgtgcaac tggcgctgag aaaagccatt gccgaaagcg
      241 ccgggaaatc cttccgcacc ggcctgaagc gcatggggct ttccgcgctg gagatcgaaa
      301 gggtctatac cgtgccgcgg gtgatcgccg gcctcttggc aagcgcgggc gagatcgtac
      361 tggcgctgct gtcagccgcc gtggtggcgg agtgggtgtt ccaccgcgcc ggcgcggccg
      421 atctgttcgt gaaatccgtc gccctgcacg actggaacat gacggcgctg atcctgtttg
      481 ttttcgccgg tctcgccttc ctggccgaaa tcctgggccg cgtcagcgcc tatgccttgg
      541 ccaacgaggg caagacatga gcgcgctcga aacccaggcc gcgcccatcc tgcgcgccgt
      601 cctgcgcctg atcggctggg gcggactcgg cctgatcgcc gtcgccgtgg cgctcagcga
      661 tttcctggcg agcgccccgg ccggcaccgt cggtctcggc ccgatcctgg ccgccccttc
      721 gccggaccac cttttcggga ccgacctgct cggccgcgac atgttgagcg agacattgca
      781 cggactgagc gtcaccgccg gccatgcgct cgtggcggcg ctggttgcca tcaccatggg
      841 cggcctgttc ggcgcggtgg ccgcccgcct gccgcgcctc ttcggcctgt cgttgcgcgg
      901 cgccatcggg gtcttcgcgg cgattccggc actgttgctc gccattctgg cgatcggcct
      961 ttccacccgt ttctatgccc ctttggccgc gggcctcgcc gccgccccgc tcgccttcat
     1021 ccgcgcctat gaccgggtgg aagcgcaaag cgattccacc catgccggat acgcgcgcgc
     1081 cacgggcatc gccgccgtca cgctgctgcg ccgcgatttg acctacgaat tccgcggcac
     1141 ggtggcggcg gcggcggcgc gcgccctggc ggcggtgacg atcatccttt ccaccgtgag
     1201 tttcctcggt ttcggcgcta ccccgccggc gcgtgacctc ggattgatga tcgctgccgc
     1261 caaggcgagc tttctccatg cctggtgggc ggcggcgttt cccgccctgg cattgatcgt
     1321 cttgatcctg tgcgcccgca tggcggcggg actggacgaa ggcgaacggc catgagcgag
     1381 cccgcagcca tcctcgacgt cgccgaactg acggtagcgc ggccgggggc ggcgccggaa
     1441 ctcgaccgct tttcgctggt gctggaagca ggcgaaagcg ccatcgtgct gggcgaagac
     1501 ggatcgggca aggaagcgct gatccgcgtg ctgggcgggt ttgccgaaag gacggatcag
     1561 atttccggta cactccgctt cggcaacgcc gaggcgcggc cggcggcgaa acgcgcgcgc
     1621 gcgcccatcc gtatcgccta tctgccgaat cctctggcgc agcccttcgc ggcgcatgcc
     1681 ggggtgctgg cgcaattgac ccgagtggtg gcgcgaaaac tggggtcacc gctcacgagc
     1741 gcgcgcgagg aactgcgcct cgccctggag cggctcgatg gcgccccgct gctggcgctg
     1801 ctgaacgaaa aaccccaggt gctcgacccg gtctccaccg cctggggctt gctggcttgc
     1861 gcctgcgccc agacccccga cctggtgctg gccgaccacg cgttcgccga tctggggccg
     1921 acggcgacgc aagcgttgct ctcggcgctg gcgacggcac gcaaacgcct cggcttcgcg
     1981 ctgctttacg ccaccggcag cttgcagccc gcggccaggc tgggcggccg cgtggtggtg
     2041 atccggcacg gacgcatggt cgaagaaggc gatgtgacgc ggctggcaag cggccatgcc
     2101 catgcctata cgaatacgct gttcaaggcg ctgccgcggc tgtcgctcga cggccagaag
     2161 agccgcaacg ccgcccgcgg tcagcccttg ttgcaggtcc acggtctcga tcttggggcg
     2221 cttggcgggc gcgaagccat cacctttgaa ctgcgccgcg gcgcctcgct ggcgctggtc
     2281 ggagaagaag gctcgggccg gcgcgcgctg gtgcgcgccg tgctggggct cgatcgcgcg
     2341 cctgccgggc gcgtggtctt cgacgcggtc gatctcaaca tcctgtcgca aaccatgacc
     2401 gcgcggctgc gccggcgcgt cgccttcatc accggggccg acgacgtgct cgatccgcgc
     2461 atgagcctgt gggacgcggt ggatgaacct ttgcgcgcgc atctcaagct gaccggcgag
     2521 ctggtcgccg gctatcgcga aaccgccttg aagcgcgtcg ggctggcctc ccatgacggc
     2581 agccggacgg tggccacgct cggcgccttc gacaagcgcc gcctgcaggt ggcgcgcgcc
     2641 atcgtcagcg ccccgctgct ggcggtggtg gacgaaccgt tgcgcggcct ggacgccttc
     2701 gcccagacga tcatgcgcga ggtgctgacc gattttcgca cccagcagga cccggcgttc
     2761 ctcgtcatca cctccgattt caccgtggcc caggcgctcg ccgacgaggt catggtcttc
     2821 aaggacggaa aaatcgtgga acgcggcccc atcgccgccg tcttgaatgc ccccaaggac
     2881 ccttgcacca agagcctgat cgaggcggtc agccttcccg gcctttcgca aaccccgcct
     2941 tcggtataga gcgcgatccg gcgaagtggc ccgtagcgcc agcgtgcggg cgcggttcgc
     3001 cgtaagatcg cgcgacaaac aaggaactaa agcaaaatcg caattctatt tgaaactatt
     3061 ttgctttaga aacggttcat gattccgcga tatgcccgcg ccgaaatggc cgccatctgg
     3121 tcggccgaga ccagattccg catctggttc gagatcgaag cccacgccgc cgacgcgatg
     3181 gcgaagctgg gcgtcattcc caaagaagcc gcggcgaaaa tctgggaaaa gggcaaagcg
     3241 gccaagttcg acatcgggcg catcgacgag atcgagcgcg aggtcaaaca cgacgtcatc
     3301 gccttcctga cccatctggc ggaaatcgtc ggccccgagg cgcgcttcct gcatcaaggc
     3361 atgacctcgt ccgacgtgct ggacacctgc ctcgccgtgc agcttcgccg cgccgcggac
     3421 attctcatcg ccgatgtcga tcttctgctc gcggcgctga agacgcgcgc cttcgagcac
     3481 aaatacaccc ctaccatcgg gcgcagccac ggcatccatg ccgagcccac caccttcggc
     3541 ctcaagctgg cgggctatta cgccgaattt ttgcgtgcga aggcgcggct cttgacggcg
     3601 cgcgaagaga tttccacctg cgcgatttcg ggcgcggtcg gcaccttcgc caacatcgat
     3661 ccgccagtgg aggcctatgt cgccggcaag ctgggcctga agccggaacc ggtttccacc
     3721 caggtgatcc cgcgcgaccg ccatgcggcc tatttcgcgg cgctcggcgt ggcggcggcg
     3781 tcgctggaac gcttggccac ggaaatccgc catttgcagc gcaccgaggt tctggaagcg
     3841 gaggagtatt tctctccggg ccaaaagggc tcctcggcca tgccgcacaa gcgcaatccc
     3901 gtgctctccg agaacgtcac cggcttggcc cgcatggtgc gcgcttatgt cgtgcccgcg
     3961 ctggaagacg tggcgctgtg gcacgagcgc gatatttcgc actcctcggt ggagcgctat
     4021 atcggcccgg acgccaccat taccctcgac ttcgcgctgg cgcgcatggc ggggatcgtc
     4081 gaaaagctga tcatctatcc caagcgcatg caggagaatc tggataagac gcgcggactg
     4141 gtgcacagcc agcgcgtgct cttggcgctg acccaaaaag ggttagggcg cgaggagtcc
     4201 tatcgcctgg tgcaaaagaa cgcgatgcgg gcgtggaacg gcgaaggggc gctgcttgat
     4261 ctgctcaagg ccgacgaaga cgtctcgcgc gcgctctcgg ccgcggaact cgaaaacctg
     4321 ttcgactttg gctatcactt caagcaggtc gatacgatct tcgcgcgggt gttcgcggag
     4381 taaccgtctt atgcggattg tcgagttgga cgctggcggc ttcaagacgc cgctcgattt
     4441 tctcgctgcc gtgcgccgcg cgattggcgc gcctcaagaa cccggcctga atatcgacac
     4501 cctcgtcggt gcgatgcttc gaggctgctc cgccgggcct tgcgtccttc gcattgtggg
     4561 gatggggaag gcgccttccc cggtcaggga ggaagctgaa gccctggtca aggccctcaa
     4621 ggacgccagc ctatggcgcc ataaccaccg ctatgaagat atcggggtgg cgatcgagat
     4681 cgtcccttaa agcatcggag gttcagttgg ggtaatatcc tgaggcagcg aagtagttgc
     4741 tgcattcggt tgggctgtag aggtcgcaga tgtcgccgat ggcgtcccag agggcgtcga
     4801 tggttcgtgc agctctggcc cgcaggcggg cttgagcttg gagcagcgcc agcccccttc
     4861 cggtttcggg cgaatttcgg aatcgaaagg acactggcaa cc
//
LOCUS       DASWPU010000368         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_119553,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000368 DASWPU010000000
VERSION     DASWPU010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119553"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1053)
                     /locus_tag="VGC27_07980"
     CDS             complement(94..1053)
                     /locus_tag="VGC27_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0282545.1"
                     /translation="MRQITFANQLGFEKYARKSRREEFLNGMDVVVPWRELEALIEPF
                     YPKAGNGRQPVGLSIMLRVYFLQQWFSLSDPGAEDALYESPVLRRFAGVDLGRAAAPD
                     ETTILRFRRLLEQHELCGQILDTVNHYLASKGLRISTGTIVDATIIAAPSSTKNSNKE
                     RDPEMHQTRKGNQWFFGAKAHIGVDSKEGIVHSVCTSAASVSDVHMLPDLLHGAEKKV
                     WGDAGYQGQTEAIHAAAPEAQDMTSRRAKYKGGVDEVERRKNRTKARVRSKVEWPFRI
                     LKRIFGYTKVRYRGLKKNHEWLLAAFALVNLYQHRKRLVLLGA"
BASE COUNT          324 a          452 c          450 g          334 t
ORIGIN      
        1 tctagtcgag cctgcaggga taaatgcccg gcacaagcga tttatctggg aagtttgtgt
       61 gcccgggtgg ccggctatcg gccttcgggc acactacgcc cccagtagga ccagccgctt
      121 gcggtgttgg tagagattga caagcgcgaa ggccgccagt agccattcgt gattcttctt
      181 caggccgcga taacgaacct tcgtgtatcc gaagatgcgc ttcaggatgc ggaagggcca
      241 ctccaccttg gagcgcactc ttgccttggt gcggttcttg cgccgctcca cttcatcgac
      301 gccgcccttg tattttgccc gccgcgaggt catgtcctgc gcctcgggtg cggccgcgtg
      361 gatcgcctcc gtttgccctt gataaccggc gtcgccccag accttcttct ccgcgccgtg
      421 cagcaggtcg ggcagcatat gcacatcgga gactgaagct gccgaagtac ataccgagtg
      481 cacgatgcct tccttgctat caactccgat gtgcgccttg gcgccgaaaa accactggtt
      541 gcctttgcgt gtctgatgca tctctggatc gcgttctttg ttgctgttct ttgtcgacga
      601 tggcgccgcg atgatcgtgg catcgacgat ggtgccggtg gaaatgcgca accctttgct
      661 ggccaggtag tggttcaccg tgtcgagtat ttggccgcac agttcgtgct gctccagcag
      721 gcggcggaag cgaaggatag tggtctcgtc cggcgccgcg gcacggccca gatcaacgcc
      781 cgcaaaacga cgcagcaccg gcgattcgta gagcgcatcc tcggcgccgg gatcggagag
      841 actaaaccac tgctgcagga agtagacgcg cagcatgatc gacaacccga ccggctggcg
      901 gccgtttccg gccttggggt agaacggctc gatcagcgct tccagttcac gccacggaac
      961 cacaacgtcc atgccgttca aaaactcttc ccggcggctc ttccgtgcgt acttttcaaa
     1021 ccccaactga ttcgcaaatg ttatctgccg catcgcgtcc tccatcctca ttcaacttta
     1081 accaggctga atgcggttgg agaacttatg cagagattcc ctaaagcaag agagaaagcc
     1141 tacagcatgg cagatggagg cggactgtac ctgtgggtga caccacccgg cggcaagctt
     1201 tggcgttggt cttatcgctt tagcggcaag cggaagctca tgacgctggg aaggtacccg
     1261 gatatttctc tcgcagatgc gagggatcgg cacggcgacg ccagaaagcg tcttgctaaa
     1321 attccgcaag agacgacctc ggtggctaaa gccgcagggt aactgccgcg tttatggcac
     1381 gggcaaacct gtgcccttaa gcagaacaaa tgcgagttta tcggttggat tgagctatta
     1441 aacaggtttt taaagcccgt caaaaaactc atgcgagtac cgcgagagat gacatcagcg
     1501 gctaaacagg ctgcggaaaa acgcggtatt gccacgagaa gattccctcg gcaattgctc
//
LOCUS       DASWPU010000369         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_27377,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000369 DASWPU010000000
VERSION     DASWPU010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27377"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VGC27_07985"
     CDS             <1..865
                     /locus_tag="VGC27_07985"
                     /inference="COORDINATES: protein motif:HMM:NF021621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEY0282546.1"
                     /translation="VMDGAQARFFALRKNGDGQVFEEAAEALNADLPRYSREDGSDKP
                     ERAFAVGKARGVVEPRHDYIKLAKHNFAREVAGMLDAALADRRYDRLVLVAPPRSLGE
                     LRELLSERVKASLAHEVPKNLTKLGADALRKKLSGFQVTAAAPVGAPVLRAEAKETTK
                     AKVSLPVSVVFRNMAESPTVHSTALKQAAKLGRKYGRIVRCKVTVEAPHRHHRKGRLF
                     LANVDLALPGRETTTKGTGKNRHAHEDVNVALRDAFDAASRQLQDHFRRKQGAAAQTR
                     YGFGVGEAEEA"
     gene            complement(874..1098)
                     /locus_tag="VGC27_07990"
     CDS             complement(874..1098)
                     /locus_tag="VGC27_07990"
                     /inference="COORDINATES: protein motif:HMM:NF022456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HEY0282547.1"
                     /translation="MHPLSPHVKKLIGTLITLVWLLVYTLLAMGIGVHVLPHANWLVE
                     LLYYVLAGALWIVPVGLMLPWMYREPKPKD"
     gene            1168..2253
                     /locus_tag="VGC27_07995"
     CDS             1168..2253
                     /locus_tag="VGC27_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY0282548.1"
                     /translation="MTAFSAVAPQSQSGRRAVGIWLLAVAALIVAMVAVGGLTRLTGS
                     GLSITQWDPIMGALPPMGDAAWHDAFAKYQQIPQYRLENQGMSLEGFKAIFWWEWAHR
                     LLGRLIGFAFFVPFVWFAWTGAIARKEWPRMVLLFALGGLQGFVGWWMVESGLETRVS
                     VSQYRLAIHLGVAVILLGAILWTALEYLRHPPPDASSLRSTHRPLHKGEAKAAFAFVG
                     LVYLQILLGALVAGLHAGLIYNTWPLMDGRLFPEHPFFASPWWINFGENPGLAQFNHR
                     IAAYLLAVCAYLLWSWGRRIGLQGAARRSTDAVLYTMLIQVALGIFTLLWHVPIMLAV
                     LHQVVAALLFCAAIWQAFELRYASPKP"
BASE COUNT          395 a          705 c          762 g          425 t
ORIGIN      
        1 ggttatggac ggcgcgcagg cgcgcttctt tgcgttgcgc aagaacggcg acggacaggt
       61 tttcgaggag gccgcggaag cgttgaacgc cgatctgccg cgctactcgc gcgaggacgg
      121 cagcgacaag cctgagcgcg ccttcgctgt cggcaaggcg cgcggcgtcg tggagccgcg
      181 ccacgactat atcaagctgg cgaagcacaa tttcgcgcgc gaggtggcag gcatgctcga
      241 cgcggcgctc gccgatcgcc gctacgaccg gcttgtactg gttgcgccgc cgcgcagtct
      301 cggcgagttg cgcgaactgc tttcggagcg ggtgaaagcg agcctggcgc acgaagtgcc
      361 caagaacctg acgaagctcg gcgccgacgc cctgcggaag aaactgtccg gctttcaggt
      421 gacggccgcg gcgccggttg gcgctccggt tttgcgcgcc gaagcgaagg agacaacgaa
      481 ggcgaaagtc tcgctgcccg tatccgtcgt gttccgcaat atggcggaat cgccgaccgt
      541 tcattcaacc gcgctcaaac aggcggccaa gctcggccgc aaatacggcc gcatcgtccg
      601 atgcaaagtg acggtcgagg cgccgcatcg gcatcaccgc aagggcaggc tgttcctggc
      661 caatgtcgat ctggcgctac cgggccgcga gaccacgacc aaaggcacag gcaagaacag
      721 gcacgctcac gaagacgtca atgtggcgtt gcgcgacgca ttcgacgccg cgtcgcgcca
      781 gttgcaagac cacttccggc gcaagcaggg cgccgccgcc cagacgcggt acgggttcgg
      841 agtgggcgag gccgaggaag cgtagcgttc gggtcaatct ttcggcttcg gctcgcgata
      901 catccagggc agcatcaggc cgaccgggac gatccataac gcgcctgcga gcacgtagta
      961 gagaagctcg accagccaat tggcgtgggg cagaacatgc acgcctatgc ccatggcgag
     1021 caaggtataa acgagcagcc acaccagcgt gatcagcgtg ccgatcaatt ttttgacgtg
     1081 gggcgacagc ggatgcatgg ggtatatgga cctcaagggc catgagagct atcaagcgcg
     1141 tgccttgaaa cgcaagcccg gaatcgcatg accgcattct ccgccgtggc cccgcagtcc
     1201 cagtccggcc gccgcgcggt gggcatttgg ctgcttgccg tagcggcgct catcgtggcc
     1261 atggtggcgg tgggcgggct gacgcggctg acgggttccg gcctttcgat cacccagtgg
     1321 gatcccatca tgggcgcgct gccgcccatg ggcgacgcgg cctggcatga cgccttcgcg
     1381 aagtaccagc agattcccca gtaccggctg gaaaaccagg gcatgagtct ggaaggcttc
     1441 aaggccatct tctggtggga atgggcccat cgcctgctcg ggcggctaat aggttttgct
     1501 ttcttcgtgc cgttcgtctg gtttgcctgg acgggcgcga tcgcgcgcaa ggaatggccg
     1561 cgcatggtgc tgctgttcgc gctcggcggc ctgcaaggct tcgtcggctg gtggatggtg
     1621 gagagcgggc tggagacgcg ggtttccgtc agccaatacc gcctggcgat ccatctgggt
     1681 gttgcggtca ttcttctggg cgcgatcttg tggacggcgc tggaatatct gaggcatccc
     1741 ccacccgacg cgtcttcgct gcgctcgacg catcggcctc tccacaaggg ggaagcgaaa
     1801 gcggcgttcg cattcgtcgg gcttgtctat ctccagatac tgctcggcgc cttggtggcg
     1861 ggactgcacg caggcctgat ctacaacaca tggcctttga tggacgggcg cctgttcccc
     1921 gaacatccgt tctttgcctc gccatggtgg atcaatttcg gcgagaatcc ggggttggcg
     1981 caattcaatc accgcatcgc ggcttatctg ctggccgtgt gcgcttacct gctttggtcg
     2041 tggggcaggc gcatcgggct tcaaggcgcc gcacgccgca gcaccgacgc cgtactgtac
     2101 accatgctca ttcaggtcgc gctcggcatc ttcaccttgc tgtggcacgt gcccatcatg
     2161 ctggcggtat tgcatcaggt ggtcgcggcg ctgctgttct gcgccgcgat atggcaggcg
     2221 ttcgagctgc gctacgcttc ccccaagccc tgatccagat cggcgatgat gtcgtcgacg
     2281 ctttcga
//
LOCUS       DASWPU010000370         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_70909,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000370 DASWPU010000000
VERSION     DASWPU010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70909"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..845)
                     /locus_tag="VGC27_08000"
     CDS             complement(135..845)
                     /locus_tag="VGC27_08000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0282549.1"
                     /translation="MRLLYCHYMLADQRKRFESVIRYIQSIGEFIGTDQVLDVLHGRA
                     PLERNSFHLSFDDGMKSVFANALPILRAHGVPAILFVPTVVISAPSDQFEEIRRRLVT
                     SKTDVEVATWDDLEKARAAGFEIGSHTRTHARLSDVLASKSAIEDEIFGSKMDLERRL
                     GGECRYISWPYGRLQDVNEASLDVVRKAGYKACFGAFRGRVVPTVTSAFRIPRHHFEP
                     DWPLAHVKCFAHGAWEAA"
     gene            complement(1148..>1861)
                     /locus_tag="VGC27_08005"
     CDS             complement(1148..>1861)
                     /locus_tag="VGC27_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282550.1"
                     /translation="SVDRAWYGYPLLHSAWAARKLGHKRISAIEFGVAGGNGLVSLER
                     HAERVHQETGVEVAVYGFDTGEGMPQPQDMRDMPYLWQSGYFAMDQAKLKARLRVAKL
                     VLGQVEDTVAQFFAKENPPPIGFISFDLDYYSSTVAALKILDAEHARYLPRVVCYFDD
                     LAGGISDAYNEFAGELLAIKEFNDAHADIKISRVRGLSYHSKNIPAQWHEKIYVTHRF
                     AHPDYGKPTSDTSELALHD"
BASE COUNT          392 a          566 c          517 g          386 t
ORIGIN      
        1 gtcgcgcgcg atttggcgct ttggcgcggg aaacaatcac tctcgcaacg ctattatttc
       61 cgcttgcgag gagcatcggg atacattcgg aagcgatgcc gaaccgactt gacgtcagta
      121 cgacaagctt catgtcacgc cgcttcccag gcgccgtgcg cgaagcactt cacatgcgcc
      181 aacggccaat cgggttcgaa gtggtgcctt ggaatgcgaa acgcgctcgt gaccgtgggg
      241 acgacccgcc cgcgaaacgc cccgaaacac gccttatagc ccgctttccg gacgacatcg
      301 agcgaagcct cgttgacgtc ctgcaatcgt ccatatggcc acgatatgta acggcactcg
      361 ccgcccaggc ggcgttccaa gtccattttc gatccgaaga tctcatcttc aatggcgctt
      421 ttggatgcca ggacatccga aagccgtgcg tgggtccgtg tgtgcgaccc gatctcgaac
      481 ccggccgcgc gcgccttttc caggtcatcc catgtggcga cctcaacgtc cgtcttggac
      541 gttaccaatc tgcggcggat ttcctcgaac tggtcgctgg gcgcggagat aacgactgtc
      601 ggcacaaaca ggatcgccgg cacgccatgc gcacgcaaga tcggcagagc attcgcgaag
      661 acgcttttca tcccatcgtc gaacgaaaga tggaaggaat tccgctccaa cggcgctctg
      721 ccgtgcagga catccagcac ttggtccgtg ccgatgaatt ccccgatgct ctgtatgtac
      781 cggataacgg actcgaaccg tttcctctga tcggccagca tatagtgaca atagagcaac
      841 cgcaaaacgg gcgtcttcgc ggggcgcaag aagaggcttt ggaccgacaa ggcgccgtgc
      901 cgcgccaatt cccttagact gcctagcccg tatgtctgac tacgcacgtc gctgtagctg
      961 actgcattca aggatgttcg cccattcttg aaggtttcac agaggcaaaa aaccgcgacc
     1021 atcttcaaaa cgagactatt ggttaggccg tctcgattgc ccacagtcca acagacagtt
     1081 catcgatgca gggtttatga caaccgatgc ctatgttgtt tgatcgtgcg catcggcttt
     1141 cccgccgcta atcatgcagc gccagttcac tggtatccga cgtcggtttg ccgtaatcgg
     1201 ggtgcgcgaa ccggtgggtg acgtagattt tttcatgcca ctgcgccggt atattcttgg
     1261 agtggtagct caacccccgg acacgcgaaa tcttgatgtc cgcatgagcg tcgttgaatt
     1321 ccttgatggc cagaagctcg ccggcaaact cgttgtaggc gtcggaaatc ccgccggcga
     1381 ggtcgtcgaa atagcagaca acccgaggca aatagcgcgc gtgttccgca tcgaggattt
     1441 ttagcgccgc aacggtcgat gaatagtaat cgagatcgaa ggatataaag ccaatcggcg
     1501 gcgggttctc cttggcaaaa aactgcgcca ccgtatcctc gacctgtccc agcaccagct
     1561 tggcaacccg tagccgcgct ttcagcttgg cctggtccat cgcgaaatat ccgctctgcc
     1621 agaggtaggg catgtctcgc atgtcctgcg gctgtggcat cccctctccc gtgtcgaagc
     1681 catacactgc aacctcgacg ccggtttcct gatgcacgcg ctcggcgtgc cgttcgaggg
     1741 atacgaggcc gttgccgccg gcgacgccga attctatcgc ggagatgcgc ttgtgaccca
     1801 gcttgcgcgc ggcccacgca ctatgaagaa gcggatagcc gtaccaagcg cgatccaccg
     1861 a
//
LOCUS       DASWPU010000371         6329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_40390,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000371 DASWPU010000000
VERSION     DASWPU010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40390"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..354
                     /locus_tag="VGC27_08010"
     CDS             73..354
                     /locus_tag="VGC27_08010"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEY0282551.1"
                     /translation="MAKLTGPRELHFTAHAENMLHERGIRREWVTAALTEPDWTASDP
                     HPDRIRVFRAIPDAGGKILRVVYSKAGSKFWVVTVFFDRNAERPEGTGA"
     gene            351..551
                     /locus_tag="VGC27_08015"
     CDS             351..551
                     /locus_tag="VGC27_08015"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEY0282552.1"
                     /translation="MKVSFDRETDALYVRFSDDAVSETAEVRPGVMFDYAADGRIVAF
                     EILDASRTLAANDMKKLAIEVA"
     gene            complement(823..1413)
                     /locus_tag="VGC27_08020"
     CDS             complement(823..1413)
                     /locus_tag="VGC27_08020"
                     /inference="COORDINATES: protein motif:HMM:NF027558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HEY0282553.1"
                     /translation="MRKLELFAIASLLLVATAANAGWQDRASRFDAQRLSRLDESRAK
                     GLNEAQGAPAADLAVIRSVLDAQAVAASPSALQGTWRCRTIKLGGITPDIIYSWFNCR
                     ISPKGGALAFEKLTGSQRSSGLLYPEGGGFVYLGASYVTGEKPNAYSGNGAEAGAAAT
                     PDDQIGYLSLLADGRARIEFPYPLQESTFDVLELKR"
     gene            complement(1400..2245)
                     /locus_tag="VGC27_08025"
     CDS             complement(1400..2245)
                     /locus_tag="VGC27_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0282554.1"
                     /translation="MAETALLKGRRGLVMGVANDHSIAWGIARVLAAQGAELAFTYQG
                     EAQAKRLKPLAASIGSALIFPGDVENDGELDAAFATLKREWGGLDFLVHAIAYSDKNE
                     LKGRYVDTSRENFVRTLSVSCYSFTAVARRAARLMTGTGGAMLTLTYLGAGRVMPNYN
                     VMGVAKAALEASVRYLAADLGRDNIRVNAVSAGPMRTLAGSAVGDGRTMFRWIRALNP
                     LHRTIELDHVGGAALYLLSELSAGVTGEVHFVDAGYNVIGVPPASVLKSWNAAENGDK
                     EDAQT"
     gene            complement(2501..3712)
                     /gene="fabB"
                     /locus_tag="VGC27_08030"
     CDS             complement(2501..3712)
                     /gene="fabB"
                     /locus_tag="VGC27_08030"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEY0282555.1"
                     /translation="MRRVVVTGMGIVSSIGNNTQEVTASLREAKPGIAAAPVYRDMGF
                     RCQVEGPVRIDLDAAIDRRARRFMGDTAAYAFLAMDQAIRDSGLEEKDIVNEKTGLIV
                     GSGGPSTKTLIWAADTARNASPKRVGPFAVPKAMSSTCSAVLSTWFKIKGVNYSISSA
                     CATSAHCIGNASELIQFGKQDVIFAGGGEELDWTLSVLFDAMGAMSSKFNETPQKASR
                     AFDKNRDGFVIAGGGGVVVLEEYEHAKARGAKIYGELVGYGATSDGHDMVAPSGEGAV
                     RCMRGALATVKGPIDYINAHGTSTPVGDVTEIKALREVFGGKIPPLNSTKSLAGHSLG
                     ATGAQEAIFSLLQMQNGFIAESANIEELDPEIGDAPVVRSRIDNAKLERVLSNSFGFG
                     GTNATLVFQRC"
     gene            complement(3820..4377)
                     /gene="fabA"
                     /locus_tag="VGC27_08035"
     CDS             complement(3820..4377)
                     /gene="fabA"
                     /locus_tag="VGC27_08035"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEY0282556.1"
                     /translation="MKDQDKVDKLNPARRTSFDLAGLLACARGELFGHGNARLPLPPM
                     LMFDRITKICERGGSHDKGEVTAELDVHPDLWFFKCHFQDDPVMPGCLGLDAMWQMVG
                     FFLGWLGGMGKGRALGVGEVKFSGMVLPSNSLVSYYIDFKRVIMRRLVVGIADGIMKV
                     DGKVIYEAKDLRVGLFGDVAQGAPA"
     gene            4534..4959
                     /locus_tag="VGC27_08040"
     CDS             4534..4959
                     /locus_tag="VGC27_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY0282557.1"
                     /translation="MRRKSRELPNETAVARLRQAGLRPTRQRNELAKLLFRNGHRHLT
                     AEALHAEAVSEGIKVSLATVYNTLHQFTQAGLLLQVVVDPTRSYFDTNTLDHQHFYHE
                     DDGTLTDIPGRQIAVECLPTPPPGMEIKRVDVVVRVRRQ"
     gene            5087..5509
                     /locus_tag="VGC27_08045"
     CDS             5087..5509
                     /locus_tag="VGC27_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEY0282558.1"
                     /translation="MATLKGSKTAENLREAFAGESQANRRYLYFAQKADVEGLNDVST
                     VFRSTAEGETGHAHGHLEYLEAVGDPATGEPIGDTALNLKAAIVGETHEYTDMYPGMA
                     KTARSEGFDEIADWFETLAKAEKSHAGRFQKALDTLGK"
     gene            5580..>6329
                     /locus_tag="VGC27_08050"
     CDS             5580..>6329
                     /locus_tag="VGC27_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY0282559.1"
                     /translation="MSQEGSLGAPTRHPIAWRSDDFYDEAKLDAELRRVFDICHGCRR
                     CFNLCDSFPRLFDLIDSDPNENVESLKSEDFARVVEACTLCDMCFMTKCPYVPPHAFD
                     LDFPHLMLRHRAVQARQGHKSFTQGQLAEMDRNGRLARFVSPAVNWASAKSNAPMRAV
                     LEKTAGIDRDAPLPKFHTKTFVAADRSDPIAPNRDAPAFGKRKAALYATCFVNYNKPA
                     TGMAARAVLNHLGVETVTAYPGCCGMPFLEQA"
BASE COUNT         1251 a         2019 c         1912 g         1147 t
ORIGIN      
        1 ggtgtagggt ttgacacggc gccgcgcgcc atcgttttcg cttaagatga aacaacgaat
       61 tgggaatgcg ccatggccaa gttaaccggc ccgcgtgaat tgcacttcac ggcccacgcc
      121 gaaaacatgc tgcacgaacg cgggatccgg cgcgaatggg tcaccgccgc ccttaccgaa
      181 cccgactgga ctgcctccga tccccatccc gaccgaatcc gcgtttttcg ggcaattccc
      241 gatgccggag gcaaaatcct acgggtcgtg tatagtaagg ccggtagtaa attctgggtc
      301 gtgaccgtat tcttcgacag gaatgctgaa aggcccgaag gaactggcgc atgaaagtga
      361 gttttgaccg cgaaaccgac gcactctatg tgcggttctc cgacgatgcc gtctcggaaa
      421 cagcggaggt gcgccctggc gtgatgttcg attatgcggc cgacggccgg atcgtggcat
      481 tcgaaatcct cgacgcgtcg cggacccttg ctgccaacga catgaagaaa cttgcgatcg
      541 aggtcgcgta ggcaagggcg cgggttagcg ctgttttgtt ctgcacgaat ctcacaccac
      601 tatcatttcg tcatggcctg gcttgacccg gccatccagt gaaaagaaag agtctttcgg
      661 cgcggacgcg cgccggctgg gtggcccgct tttgcgggcc atccaggtga caacagatca
      721 attctcataa agtgtactga gatttgcgta cgatcttcgg gtgcggcgtc cgtcctcaac
      781 tgggtggccc gcattcgcgg accatgacaa gtgagttgag agctatcgct tcagttccag
      841 cacgtcgaag gtcgattcct ggagcggata agggaactcg atccgcgccc ttccgtcggc
      901 gagcagcgac agatagccga tctggtcgtc gggcgtggcg gcagcccccg cttcggcgcc
      961 gttgcccgaa taggcgttgg gcttctcgcc ggtgacatag gaggcgccga gatacacgaa
     1021 gccgccgccc tcgggataaa gcaagccgga gctgcgctgg ctgcccgtca gcttctcgaa
     1081 agcgagcgcg ccacccttcg gcgatatgcg gcagttgaac caggaataaa tgatatcagg
     1141 ggtaatgccg ccgagcttga tggtgcggca gcgccaagtg ccttggagcg cggagggcga
     1201 agccgccacg gcctgggcgt cgagcaccga acggatgacg gcaagatcgg cggcgggcgc
     1261 gccctgggct tcgttcagac ccttggcgcg ggattcgtca agccgcgaaa ggcgctgcgc
     1321 gtcgaaacgc gaggcccggt cctgccagcc ggcgttcgcc gccgtggcga cgagcagcag
     1381 agacgctatc gcgaagagtt caagtttgcg catcttcctt gtctccattc tccgccgcgt
     1441 tccagctttt gagaacggac gccggcggca cgccaatcac attgtagccc gcatcgacga
     1501 aatgcacttc gccggtcacc ccggccgaaa gctcgctgag cagataaagc gcggcgccgc
     1561 cgacatgatc gagttcgatg gtccggtgca gcggattgag cgcgcgaatc catctgaaca
     1621 tcgtgcgccc gtcgccgacg gcggaacccg ccagcgtgcg catcggcccg gcgctgaccg
     1681 cgttgacgcg gatgttgtcg cgccccagat cggccgcgag atagcgcacc gacgcttcca
     1741 gcgccgcctt ggccacgccc atcacattgt aattcggcat gacgcggccg gcgcccaaat
     1801 aggtcagggt cagcatcgcg ccgcccgtgc ctgtcatcaa ccgcgccgcc cgccgcgcca
     1861 cggcggtgaa ggaatagcag gagaccgaca gggtgcggac gaagttctcg cgcgaagtat
     1921 cgacgtagcg gcccttcagc tcgttcttgt ccgaataggc gatggcgtgg accaggaaat
     1981 cgagcccgcc ccattcgcgc ttcagcgtcg cgaaggcggc gtcgagttcg ccgtcgtttt
     2041 cgacgtcgcc cggaaaaatg agggcggagc cgatcgaggc cgccagcggt ttcagccgct
     2101 tggcctgcgc ctcgccctga taggtgaagg caagctccgc cccctgggcg gcgaggacgc
     2161 gcgcaatgcc ccaggcgatg gagtggtcgt tcgcgacccc catcaccaat ccgcgacggc
     2221 ccttcagcaa ggctgtttca gccatgaatc ctcatgtttc aaaatggcac gcgatgcggc
     2281 catctcccca tcgtcatgca attactatac gcggaaaacg accccgtcgt caggcgtagt
     2341 ggagcggatt tgacattcgc taccctgtct gacgcgaaca ctgtaagcga atgtcaaatc
     2401 caaagctcca ctagaatcct agagttgcta gtggtccttt gattccaaca ttcgcaaaag
     2461 tgcccggcgc aaacgggatg cgaatgttgg aatcggacca ctagcagcgc tggaacacca
     2521 gcgtggcgtt ggtgccgccg aagccgaagc tgttggacag cacgcgctcg agcttggcgt
     2581 tgtcgatccg gcttctgacc accggcgcat cgccgatttc cgggtccagt tcttcgatgt
     2641 tggcgctttc ggcgatgaag ccgttctgca tctggagcag cgagaagatc gcttcctgcg
     2701 ccccggtggc gcccagcgaa tggccggcca gcgatttggt ggaattgagc ggcgggattt
     2761 taccgccgaa cacttcgcgc aacgccttaa tttcggtcac gtcgcccacc ggcgtcgagg
     2821 tgccatgggc gttgatataa tcgatcggcc ctttcacggt ggcgagcgcc ccgcgcatgc
     2881 agcgcaccgc gccctcgccc gacggcgcca ccatgtcgtg gccgtcggag gtggcgccgt
     2941 agcccaccag ctcgccgtag atcttggcgc cgcgcgcctt ggcgtgctca tactcttcca
     3001 gcaccaccac gccgccgccg ccggcaatga cgaagccgtc gcggttcttg tcgaaggcgc
     3061 gcgaggcctt ttgcggcgtc tcgttgaatt tcgacgacat cgcgcccatg gcgtcgaaca
     3121 gaaccgacaa ggtccagtcg agttcctcgc cgccgccggc gaagatcacg tcctgcttgc
     3181 cgaactggat cagttcggag gcgttgccga tgcaatgggc gctggtggcg caggccgagc
     3241 tgatcgaata gttgacgcct ttgatcttga accaggtgga cagcaccgcc gagcaggtgg
     3301 acgacatcgc cttcggcacc gcgaaggggc cgacgcgctt gggactggcg ttgcgcgccg
     3361 tatccgcggc ccagatgagc gttttggtgg acggcccgcc agaaccgacg atcaggccgg
     3421 tcttctcgtt gacgatgtcc ttttcctcga gcccggaatc gcggatcgcc tgatccatcg
     3481 ccaggaaggc ataagcggcg gtgtcgccca tgaagcggcg ggcgcgccgg tcgatcgcgg
     3541 cgtcgagatc gatcctgacg ggaccttcca cctgacagcg gaagcccatg tcgcgataga
     3601 ccggagcggc ggcgatgccg ggcttggctt cgcgcagcga ggcggtgact tcctgggtgt
     3661 tgttaccgat cgatgagacg atgcccattc ccgtgacgac gacgcgcctc atggacggat
     3721 tccttccgtt tagccgttct catgctgcgg caggctccgg catgagagat tcaggtgtca
     3781 tcgcacccgc cgcctgtcga gggcgggcga tagcggcgtt caggccggcg cgccttgcgc
     3841 gacgtcgccg aacaggccga cgcgtaaatc cttggcttcg tagatcacct tgccgtcgac
     3901 cttcatgatg ccgtcggcga tgccgaccac gaggcggcgc atgatgacgc gcttgaagtc
     3961 gatgtagtag ctgacgagcg agttcgacgg cagcaccatg ccgctgaatt tcacttcgcc
     4021 cacgcccagc gcgcggcctt tgcccatgcc gccgagccag ccgaggaaga agccgaccat
     4081 ctgccacatc gcatccagac ccagacagcc cggcatcacc ggatcgtctt gaaaatgaca
     4141 tttgaaaaac cacagatcgg gatgcacatc caactcggcc gtgacctcgc ccttgtcatg
     4201 actgccgccg cgctcgcaga ttttcgtgat tcggtcgaac atcagcatgg ggggcagcgg
     4261 caagcgcgca ttgccatggc cgaaaagctc gcccctggcg caggccaaga ggccggccaa
     4321 gtcgaaactg gttctccgcg cgggattcag cttgtccact ttgtcttgat ccttcaacac
     4381 cttagaagag gactcccagt taacggcccc ttgcgacatt tgtacgttag aaacattcta
     4441 acggttgctg tacaatccta gcaaggatga agcccgagcg acatagatac aagggcctcg
     4501 agatgcgagt cttttcaagg acaacaagtt ctcatgcgta gaaaatcgag ggaactcccc
     4561 aacgaaacgg ccgtcgcgcg tctgcgtcaa gccgggctgc ggccgacgcg tcagcgcaac
     4621 gagctggcca aattgctgtt ccgcaacgga catcgccatc tcaccgccga ggcgctgcac
     4681 gccgaagctg tcagtgaggg catcaaagtg tcgctcgcca ccgtgtacaa cacgctgcac
     4741 cagttcaccc aggcgggatt gctgctccag gtggtggtcg atccgacccg cagctatttc
     4801 gatacaaaca ccctcgatca tcagcacttc taccacgagg acgacggcac cctgaccgac
     4861 attccgggcc ggcagatcgc ggtcgagtgt ctgcccaccc cgccgccggg catggaaatc
     4921 aagcgtgtcg atgtggtggt gcgggtgcgc cggcagtgag cgaagctgca actcactctc
     4981 tttagaataa ttctaaacta ttgacttggg aggcgtgcgg aagcatcttt gcctctgcaa
     5041 ttgcgtgccc gcggcgtatg cccagcacaa cccaaaggag agcagcatgg cgacattgaa
     5101 aggcagcaag accgcagaga atctcaggga agcttttgcc ggcgaaagcc aggccaaccg
     5161 gcgctacctc tatttcgccc agaaggccga cgtcgaaggc ctcaacgacg tctccaccgt
     5221 gttccgttcc accgcggaag gcgaaaccgg ccacgcccac ggccatctgg aatatctcga
     5281 agccgtcggc gacccggcca ccggcgagcc gatcggcgac acggccctca acctcaaggc
     5341 cgccatcgtc ggcgagaccc acgaatatac cgacatgtat ccgggcatgg ccaagacggc
     5401 ccgttccgaa ggtttcgacg aaatcgccga ttggttcgag accctggcca aggcggaaaa
     5461 aagccacgcc ggccgtttcc aaaaggcgct cgacacgctg ggcaagtaac aaaaagcgag
     5521 tggcgaatag cgaatgatat tcattactac tcgctattcg ctcctcgcgc ggcaacatca
     5581 tgtcccaaga aggcagcctc ggcgccccga cccgccaccc catcgcatgg cgcagcgacg
     5641 atttttacga cgaagcgaag ctcgatgccg aactgcggcg cgtcttcgat atctgtcacg
     5701 gctgccggcg ctgcttcaat ctgtgcgatt ccttcccgcg cctgttcgat ctgatcgatt
     5761 ccgacccgaa cgaaaacgtc gagagcctga agtcggaaga tttcgcgcgc gtcgtcgaag
     5821 cctgcacgct gtgcgacatg tgcttcatga caaaatgccc ttatgtgccg ccgcatgcct
     5881 tcgatctcga ttttccccat ctgatgctgc ggcaccgggc ggtgcaggca cggcaaggtc
     5941 ataagagttt cacccaaggc cagctcgccg aaatggaccg caacggcagg ctggcgcgtt
     6001 tcgtttcacc cgccgtcaac tgggcctccg ccaaaagcaa cgcgccgatg cgcgcggtcc
     6061 tggagaaaac ggccggcatc gaccgcgacg cgcccttgcc gaaattccat accaagacct
     6121 tcgtcgcggc cgaccgcagc gatccgattg cgccgaaccg tgacgcgccc gccttcggca
     6181 agcgcaaggc ggcgctctat gccacctgct tcgtcaacta caacaagcct gccaccggca
     6241 tggcggcgcg cgcggtgctc aatcacctcg gcgtcgaaac cgtgaccgcc tatcccggct
     6301 gctgcggcat gccgttcctg gaacaggcc
//
LOCUS       DASWPU010000372         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_2957, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000372 DASWPU010000000
VERSION     DASWPU010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2957"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VGC27_08055"
     CDS             <1..242
                     /locus_tag="VGC27_08055"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY0282560.1"
                     /translation="ADGCSGRSHNTGTAIGAVGGGLIGSALTHGSAVGVVGGAVAGGL
                     AGNSIARDSDCNDGRSVKQNRKYYYDRQHRKHYYK"
     gene            525..1604
                     /locus_tag="VGC27_08060"
     CDS             525..1604
                     /locus_tag="VGC27_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY0282561.1"
                     /translation="MPSLFDPITAGALRLPNRIVMSPLTRSRATPDTRVPVPLMAEYY
                     AQRATAGLIISEATIVDPMGAGYAATPGIYSDEQVEGWKPVVKAVHDAGGRIVLQLWH
                     VGRISDPSFLDGKPPVSASAIAAKGDVSLLRPKRPFPVPRALETEEIPGIVAAFRKGA
                     ANAKAAGFDGVELHGANGYLLDQFLQDSTNHRTDAYGGSREKRARLMLEATDAVSEVF
                     GADRVGMHLAPRSDTHDMGDSDRLGTFTYVARELGKRKIAFICAREARKEDSIGPQMK
                     EAFGGVYIANEGFTKETAEQALADGVADAVAFGKDYIANPDLVARFKTYAPLNPWVME
                     TFYSGGPKGYTDYPALAKSVLQPSY"
     gene            complement(1587..1841)
                     /locus_tag="VGC27_08065"
     CDS             complement(1587..1841)
                     /locus_tag="VGC27_08065"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0282562.1"
                     /translation="MQLETLVIWLVIGAVAGLLAGLVVKGGGFGLFGDIVVGIVGAFF
                     GGWLLPRLGINLGVGLVPIIVSATIGAVVLLLLLRLVRRL"
     gene            2023..2337
                     /locus_tag="VGC27_08070"
     CDS             2023..2337
                     /locus_tag="VGC27_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282563.1"
                     /translation="MGDFDQRGTFTCMASSSWTKPASRWSTVAGNSAADYALRRSISP
                     HRLEDVVGAFHMTSVLSAEGSRGIEFKKCDGRGHPSKNIENNYVAEQSGFLSFSKPIF
                     LR"
     gene            2434..>2997
                     /locus_tag="VGC27_08075"
     CDS             2434..>2997
                     /locus_tag="VGC27_08075"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY0282564.1"
                     /translation="MPAFDKSKLPSRHVTEGPDRAPHRSYYYAMGLTEEEIHRPFVGV
                     ASCWNEAAPCNIALMRQAQSAKKGVKEAGGTPREFCTISVTDGIAMGHEGMKSSLVSR
                     EVIADSVELTMRGHCYDALVGIAGCDKSLPGMMMAMLRLNVPSVFLYGGSIMPGRVKG
                     KDVTVVDIFEGVGQFAAGKMPACDLDEL"
BASE COUNT          604 a          913 c          941 g          539 t
ORIGIN      
        1 atgccgacgg ttgcagcggc cgcagtcaca ataccgggac cgcgatcggc gcggttggcg
       61 gcggcttgat cggcagtgcg ctcacccatg gcagcgccgt gggcgtggtc ggcggcgcgg
      121 tcgccggcgg cctggcaggc aattccatcg cacgcgacag cgactgcaac gacggccgct
      181 cggtcaagca gaaccgcaaa tattattacg accgccagca tcgcaagcat tactacaaat
      241 aagagcgccg ccctggcgct ttagagcgca atccggcaaa gtggtcggta gcagcagcgt
      301 gccgacgcgg tttgccgtca gattgcgcga aaatcaagga atctagagct tgatccgatt
      361 taattgaatc ggatcaagct ttagccggtc ttgatacgcc gcgccgtcaa cctcgattcc
      421 ggggctggcg gcgctttggc gtgtttgcgg gcgtttcgcc accttgtcgt cgcggcgtgc
      481 atcacctatg taatgtaaca gtatctatta cagaagggtc actcatgccg tctttgttcg
      541 atcccatcac ggcgggcgcg ctgcgcctgc ccaatcgcat cgtcatgtcg cctttgacgc
      601 gctcccgcgc cacccccgac acccgcgtgc ccgtgccttt gatggccgag tattacgccc
      661 aacgcgccac ggcggggctg atcatttccg aggcgacgat tgtcgatccg atgggcgccg
      721 gctatgccgc gacgccgggc atctattcgg acgagcaggt ggaaggctgg aagccggtgg
      781 tcaaagccgt gcatgacgcg ggcggacgca tcgtcctgca gctttggcat gtcggccgca
      841 tttccgatcc cagttttctg gacggcaagc cgccggtctc ggccagcgcc attgcggcca
      901 agggcgatgt cagcctgttg cgcccgaaac gcccgtttcc cgtgccccgc gcgctggaga
      961 ccgaagagat tcccggcatc gtcgccgctt ttcgcaaagg cgcggcgaat gccaaggccg
     1021 ccggcttcga cggcgtggag ctgcacggcg ccaacggcta tctgcttgac cagttcctgc
     1081 aagactcgac caaccaccgc accgacgcct atggcggcag ccgcgagaaa cgcgcgcggc
     1141 tgatgctgga agcgacggac gccgtgagcg aggtgttcgg ggcggaccgc gttggcatgc
     1201 atctggcgcc gcgcagcgat acccacgaca tgggcgacag cgaccggctc ggcactttta
     1261 catatgttgc gcgcgaactg ggcaagcgga agatcgcttt catctgcgcc cgcgaggcgc
     1321 gcaaggaaga cagcatcggg ccgcaaatga aggaagcgtt cggcggcgtc tatatcgcca
     1381 acgaaggctt caccaaggag acggcggagc aggcactggc cgacggtgtt gccgacgcgg
     1441 tggcgttcgg caaggactac atcgccaatc ccgatctggt ggcgcgcttc aagacgtacg
     1501 cgccactcaa tccgtgggtc atggaaacgt tctattccgg cggccccaag ggctataccg
     1561 actatccggc gctggcgaag agcgtcttac agccgtcgta ctagccgcaa gagcagcagc
     1621 aacacgacgg cgccgatcgt ggcgctgacg atgatgggaa caaggccgac accgaggttg
     1681 attcccaacc tgggcaacag ccaaccgccg aagaaagcgc cgacgatgcc gacgacgatg
     1741 tcgccgaaca agccgaagcc gccgcccttg acgaccaagc cggccaacaa gcccgcgacc
     1801 gcgccgatca caagccagat aaccaacgtt tcgagttgca tgtcaccgac cataatcttg
     1861 tttcctcaca tcttgcatga aattgcctcg ccgcgcggga atcggcgctt cgttaacctt
     1921 aggcgccagg aatggagcgg ggcagactcg gaaactactc agaactggga ggcgctgaag
     1981 aatggcgatc ctgcagcgcc catcggcacg agataaggcg gcatgggcga tttcgaccag
     2041 cgcggcacct tcacttgcat ggcgtcgtct tcgtggacga agccggcatc gcggtggagc
     2101 accgtcgctg gaaattcggc ggcagattac gcattgcgcc gatccatatc gccgcatcgg
     2161 cttgaagatg ttgtcggtgc tttccacatg acttcggtat tgtcggcgga aggaagcaga
     2221 ggaattgaat tcaaaaagtg cgatggtagg gggcacccct cgaaaaacat cgaaaacaat
     2281 tatgttgccg agcagtcggg ctttttgtct ttttccaagc caatcttctt gcgctaagag
     2341 ggccttgggg ggtaggggga ggggtataaa atccgaaaaa cgaatagcta ccgcttgcgg
     2401 tattcacctt attcccacgc caggtctagc ctcatgcccg cattcgacaa atccaagctt
     2461 ccttcccgcc atgtgaccga agggccggat cgcgctccgc accgctccta ttattacgcc
     2521 atgggcctga ccgaggagga gattcatcgc cccttcgtcg gcgttgcgtc gtgctggaac
     2581 gaggccgcgc cctgcaacat cgcgctgatg cgccaggcgc agtcggccaa gaagggcgtg
     2641 aaggaagcgg gcgggacgcc gcgcgagttc tgcaccatca gcgtgaccga cggcatcgcc
     2701 atgggacatg agggcatgaa atcctcgctg gtcagccgcg aagtgatcgc cgacagcgtg
     2761 gagctgacca tgcgcgggca ttgctacgac gcgctggtcg gcatcgcggg ctgcgacaag
     2821 tcgctgcccg gcatgatgat ggcgatgctg cggctcaacg taccgagcgt gttcctctat
     2881 ggcggttcga tcatgcccgg cagggtcaag ggcaaggacg tgaccgtagt cgatatcttc
     2941 gagggcgtcg gccaattcgc ggcgggaaaa atgcccgcct gcgatcttga cgaactt
//
LOCUS       DASWPU010000373         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_64760,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000373 DASWPU010000000
VERSION     DASWPU010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64760"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..673)
                     /locus_tag="VGC27_08080"
     CDS             complement(59..673)
                     /locus_tag="VGC27_08080"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY0282565.1"
                     /translation="MMDQWAKSLLEYISAHPEVAVLVVGLTAFGESFAFVNFIVPGGA
                     VLIGAGMLVNEGLLDPAVILFAATAGAVIGDAISYWIGWKFGRVLPRLWPFRRHPEVL
                     ARGVEFFERYGQASVFIGRFFGPLRAVVPTTAGIVRMRPVPFMVATFFSALIWSPVLL
                     FSGYLLSDTLASGWSMQVKAAVILSAIVLIVLGIFGARRLFHVR"
     gene            complement(943..2388)
                     /locus_tag="VGC27_08085"
     CDS             complement(943..2388)
                     /locus_tag="VGC27_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0282566.1"
                     /translation="MNLIERLRSQRSTDVRVLKAQLDLTSQTVRSTRLSSPFWALAIA
                     WLGSNSFGLFGNADFNQAVFVPVIVSATMILSASVVSIYQYDTSESNDYERTQPWFSR
                     IVAMQAVISAAWGLMPWLLWDPASLVNHVFLACATVGVLAMLIVSRSSHMDMFLASIV
                     PLTTMAAVRFLMGNTLVDLGIAIVIPAIAVQLLVDGRRLMHRLDEDSRLRFEVEDLAR
                     ELEETRDDALRKRFEAETANASKTAFLANMSHELRTPLNAILGFSEIIAQECFGPVGS
                     TRYKEYAGDIHSSGAHLLSLINDLLDVAKIEAGKMEISPHPIDARRTFEIALKLMGIK
                     AREKRQELLISIEPDTPPLYADERALKQILINLVTNAVKFTPEGGTIKVSAGPARDGG
                     FQILCEDNGPGIPREKLDKIFTPFSQVDNRYDRQAGGTGLGLALVRGLTELHGGRAWL
                     ESEYGKGCRAYVVLPAEPPKSATEGQAVAAA"
     gene            complement(2474..>3299)
                     /locus_tag="VGC27_08090"
     CDS             complement(2474..>3299)
                     /locus_tag="VGC27_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0282567.1"
                     /translation="QLAERNTEVEAKNQEIEQARRALEEKAEELALTSKYKSEFLANM
                     SHELRTPLNAILGFSDIIAEQVLGPDNPRYRDYARDINASGSHLLDLINDILDVAKIE
                     SGKMEIDAKPLDMSLTLAGIERQMALRVREKKLHLAVTIDADAPSPIADERAFKQIAL
                     NLISNAVKFTPEAGRIDVVCRRGENEGFLLAVEDSGQGIPPDKLDTVFDAFSQIDNRY
                     DRKAGGTGLGLAMVQGLARLHGGNAWIESRVGQGTKVFVYFPLAIDRQRRAVSAHG"
BASE COUNT          638 a         1033 c         1022 g          606 t
ORIGIN      
        1 gcacgaaagg ccccggcgga aacaccgggg ccttattcgt cgggtttaac cgatacgatc
       61 accgcacgtg gaacagacgc cgggcgccga aaatccccaa aacgatcaac acaatggcgg
      121 acaggatcac cgccgctttc acctgcatgc tccagccgct tgcgagagtg tcgctcaaca
      181 ggtaaccgga aaacagcaat accggcgacc agatcagtgc cgaaaagaac gtcgccacca
      241 tgaagggtac cggacgcata cggacaattc ccgccgtggt gggtaccacg gcgcgcaagg
      301 gtccgaagaa ccgcccgata aagacgctcg cctgaccgta gcgttcgaag aactcgacac
      361 cgcgcgccag cacttccgga tgtcgccgga acggccacag gcgcggaagc accctaccga
      421 atttccagcc gatccaatat gagatggcgt cgccgatgac cgcgcccgcg gtcgctgcga
      481 acaggatgac ggccggatcg agcaagccct cattgaccag catgcccgca ccgatgagga
      541 cggcgccgcc cgggacaatg aaattgacga acgcaaagga ttcgccgaag gcagtcaagc
      601 caaccacgag caccgcgact tccggatgcg cggagatgta ctccaagagg ctcttggccc
      661 actggtccat catctctccc ggcgcttggt tccgtatccg gccgactcga ctgcgccaat
      721 cgtgcagtac agaaatctga tgacagaaat aagaggggca cggaacaatt cggcacgggg
      781 ccgaaaaacg cggcgttacg gcccgagttt tcccgatcca ggcgcggctc cgttgccgca
      841 tccggtcgag ttcaagatat actattcggt ctgatcggcc gccccaaatc gaaagagcgc
      901 gatcgcagga tcgcgctctt ttttcgcgaa ttccgtgcct gcttacgccg ccgcgacggc
      961 ctggccttcg gtggcggact tgggcggttc ggcgggaagc accacatagg cgcggcagcc
     1021 cttgccgtat tcgctttcga gccaagcgcg gccgccgtgc agttcggtca ggccgcgcac
     1081 cagcgccagc cccaggcccg tgccgccggc ctgccggtcg taacggttgt ccacctggct
     1141 gaagggcgtg aagatcttgt cgagcttctc gcgcggaatg ccggggccgt tgtcctcgca
     1201 cagaatctgg aatcccccgt cgcgcgccgg cccggccgat actttgatgg tgccgccttc
     1261 gggcgtgaac ttcaccgcgt tggtgacgag gttgatgaga atctgcttga gcgcgcgctc
     1321 gtcggcgtag agcggcggcg tgtccggctc gatggagatg aggagttcct ggcgcttctc
     1381 gcgcgctttg atccccatca gcttgagcgc gatttcgaag gtgcggcgcg cgtcgatggg
     1441 atgcggcgaa atttccatct tgccggcttc gatcttggcg acgtcgagca ggtcgttgat
     1501 caggctgagc agatgcgcgc cggaggaatg aatgtcgccg gcatattcct tgtagcgcgt
     1561 ggagccgacg ggcccgaagc attcctgcgc gatgatttcc gagaaaccca ggatcgcgtt
     1621 gagcggcgtg cgcagctcgt ggctcatatt ggcgaggaac gcggttttcg aagcattcgc
     1681 ggtctcggcc tcgaaacgct tgcgcagcgc gtcgtcgcgc gtctcttcga gttcgcgggc
     1741 gaggtcctcg acctcgaagc gcaggcgcga atcctcgtcc aggcgatgca tcagcctgcg
     1801 gccatcgacc agcaactgaa cggcaatcgc cggaatcaca atggcaatgc cgagatcgac
     1861 cagcgtgttg cccatcagga agcgcaccgc cgccatcgtg gtcagaggca cgatgctggc
     1921 caggaacatg tccatgtggc tcgaacggct gacgatcagc atcgccagga cgccgaccgt
     1981 ggcgcacgcc aggaacacgt gattgaccag actggccggg tcccacaaca gccagggcat
     2041 caggccccag gccgcgctga tgaccgcctg catcgcgacg atgcgcgaga accagggctg
     2101 ggtgcgttcg taatcgttgc tctcggacgt gtcgtactgg tagatcgaga cgaccgaagc
     2161 cgacaggatc atcgtcgccg acacgatcac cggcacgaag acggcctggt tgaaatcggc
     2221 gttgccgaaa agaccgaaac tgttcgaacc cagccaggcg atggcgagag cccagaacgg
     2281 cgaggaaagc cgcgtcgaac gaaccgtctg cgatgtaagg tcgagctgcg ctttcagcac
     2341 gcgtacgtcc gtgcttcgtt ggctgcgcag ccgttctatg agattcacgg cgtcccccgt
     2401 ttcacatctc gccttctgtc gagaagcgga tgttacgcaa cgtccgttaa aatttactga
     2461 gaatgtcgcc ggttcaaccg tgcgctgaaa ctgcgcgccg ttgccggtct atggctaacg
     2521 gaaagtaaac aaacactttg gtgccttgac cgacccggct ttcgatccac gcattgccgc
     2581 cgtgcagccg cgccaaaccc tgcaccatcg cgaggcccag gccggtgccg ccggctttcc
     2641 ggtcgtaacg gttgtcgatc tgcgaaaacg cgtcgaagac cgtgtcaagc ttatcgggcg
     2701 ggatgccctg tccgctgtct tcgacggcga ggagaaaccc ttcgttctcg ccgcgacggc
     2761 agacgacatc gatccggccc gcttccggcg tgaacttcac cgcgttggag atcagattga
     2821 gcgcgatctg cttgaatgcc ctctcgtccg ctatcggcga cggcgcatcc gcgtcgatcg
     2881 tgacggccag gtgcagcttc ttctcccgca ccctgagcgc catctgccgc tcgatgccgg
     2941 ccagcgtcag cgacatgtcg agcggcttgg cgtcgatttc cattttgccg gactcgatct
     3001 tggccacgtc gagaatgtcg ttgatgagat cgagcaggtg cgaccccgaa gcgttgatgt
     3061 cgcgcgcata atcccgataa cgcgggttgt cggggccgag cacctgctcg gcaatgatgt
     3121 cggagaaccc cagtatggca ttgagcggcg tgcgcagttc gtggctcatg ttggccagga
     3181 attcggactt gtacttggag gtgagcgcca gctcctcggc cttttcctcc agcgcgcggc
     3241 gcgcctgttc gatttcctgg ttcttggctt cgacctcggt attgcgctcg gcgagctgc
//
LOCUS       DASWPU010000374         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_27430,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000374 DASWPU010000000
VERSION     DASWPU010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27430"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..574
                     /locus_tag="VGC27_08095"
     CDS             254..574
                     /locus_tag="VGC27_08095"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEY0282568.1"
                     /translation="MSNVEGSPQPISDASKLMMFEAQKKSMGVAYLLLIFLGGFGAHR
                     FYLGRTGSAVAMLIIFLLSILLSVVGIGLLGFLAIGIWCFIDLFLIPGIARQHNMNLA
                     NMLR"
     gene            complement(719..1009)
                     /locus_tag="VGC27_08100"
     CDS             complement(719..1009)
                     /locus_tag="VGC27_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY0282569.1"
                     /translation="MPMKNPPHPGQSVRLDCLEPLGLSVTDAAKALGVTRQALNNLVN
                     GKSGISPEMAIRLDKAFGGGAEVWLGLQMDYDLAQAMKNERAIKVRRMREAA"
     gene            complement(1021..1299)
                     /locus_tag="VGC27_08105"
     CDS             complement(1021..1299)
                     /locus_tag="VGC27_08105"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0282570.1"
                     /translation="MIRRFRHRGLKRLYENDDRRGLNPQHIGIVARILARLDRASEPR
                     HMDLPGWRLHPLKGEFAGFWSVTVRANWRIVFRFENGDVTDVDYLDYH"
     gene            complement(1332..1913)
                     /locus_tag="VGC27_08110"
     CDS             complement(1332..1913)
                     /locus_tag="VGC27_08110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0282571.1"
                     /translation="MRRLGFLLLLPVLLFAGRAAAQQAAGPVAQIDTSLGSITIALDR
                     EHAPLTVANFIRYAKGGHFDGTVFYRVVPGFVIQAGSTKADGSAKPTHLRPIPLESGL
                     SNLRGSVAMARSDEPASATAEFFVNLVDNTRLDPKPEAAPGTTGYAVFGQVTAGMEVV
                     DAIAAVPLGGGKGAFPDAAPKTPVLIKTVTVHD"
     gene            2139..>2324
                     /locus_tag="VGC27_08115"
     CDS             2139..>2324
                     /locus_tag="VGC27_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282572.1"
                     /translation="MAETVKNQPKKPVKAPERKAFKYPSEDEAFVRRLGSAVLARWSE
                     LPEELRAVILAEACTVWD"
BASE COUNT          448 a          736 c          674 g          466 t
ORIGIN      
        1 attatcgtgc gcatcccgcg caatcgcccc cgccagaccg aagccaagac tttgcaagcc
       61 gaagcacccg gcacgctcgc cgtatcatgc gagttcttga ggcgccttca tcgtcagacc
      121 atatgagacc cgctttccgt atactgaacc ggatttcgac cgacctgcgt cagcacatcc
      181 gcaacatgcc ttgttgacgc cgttctacat ctgcgcgaag ctcccgcaag ggttgcaagc
      241 ttaagggggg actatgagca acgtcgaagg atcgccacag ccaataagtg acgccagcaa
      301 attgatgatg ttcgaggctc agaagaagtc tatgggcgtc gcctatctac tcctcatatt
      361 tctgggcggt tttggcgctc accgctttta tctcggccgg acgggcagtg cggtcgcaat
      421 gctcatcata ttcctgttgt cgattctctt gtccgtcgtg ggtatcggac tattggggtt
      481 tctagccata ggtatctggt gctttatcga tttgttcctc atcccaggta tcgcccggca
      541 gcacaacatg aacttggcaa acatgctcag gtgagctgtt cagccgattc ggactatctg
      601 aactaaactt gccttgtcgg ccgttttgga aggtctgacg caagccggac gccgcgaaca
      661 tgccagcgat gcgtaggtcg tggctgcatt ttaccgtttc aactcatgaa gccgcgatct
      721 acgccgcttc tctcatccgc ctcaccttga ttgcgcgttc gtttttcatg gcctgcgcca
      781 ggtcgtaatc catctgcaaa cccagccaca cctctgcacc gccgccgaac gccttgtcga
      841 gacggatcgc catttccggc gaaatcccgc tcttaccgtt gacgaggttg ttcagcgctt
      901 ggcgcgtgac gcccaacgcc tttgccgcgt ccgtcacgct gaggcccagc ggctccaggc
      961 aatcgagccg gaccgattgt ccgggatggg gcgggttctt cataggcata cgcaacactc
     1021 ctagtggtaa tccaggtaat cgacatccgt tacatcgccg ttctcgaagc ggaaaacgat
     1081 ccgccaattg gcgcgcacgg tcacgctcca gaacccggcg aactcgcctt tcagcggatg
     1141 cagccgccag cccggcaaat ccatgtggcg cggttcggat gcccggtcga ggcgcgcaag
     1201 aatgcgtgca acgatcccga tatgctgcgg attcagtccg cgccggtcgt cgttctcata
     1261 aaggcgtttc aaaccgcgat ggcggaaccg ccggatcatg cgttagatat tatactgaca
     1321 attgtcactt gtcaatcatg caccgtcacc gtcttaatca gcaccggcgt ctttggcgcc
     1381 gcgtcgggga aggcgccttt gccgccgccc aagggcaccg ccgcgatggc atccaccacc
     1441 tccatgcccg ccgtcacctg gccgaacacg gcatagcccg tcgtgcccgg cgccgcctcc
     1501 ggcttgggat cgaggcgcgt attgtcaacg agattgacga agaactccgc cgtcgcgctg
     1561 gcgggttcgt cgctgcgcgc catcgccacg ctgccgcgca gattggagag gccgctttcg
     1621 agcggaatgg gacgcagatg ggtcggcttg gcgctgccgt cggccttggt gctgcccgcc
     1681 tggatcacga agcccggcac cacgcgatag aacacggtgc cgtcgaaatg gccgcccttg
     1741 gcgtagcgga tgaaattggc caccgtcagg ggcgcgtgct cgcggtcgag cgcgatggtg
     1801 atcgacccca gcgaagtgtc gatctgcgcc accggcccgg cggcctgttg cgcggcggcc
     1861 cggccggcga acagaagcac cggcagaagc agcagaaatc ccaagcgtct catgtgccgc
     1921 actccttatt cttttcggcg gcctgtttac cacgtcggtt cgtgcggcca aacagcctgt
     1981 ttttgcagca cagtttcctc gacagaagcc cgcccggccc ttataggaag gactgcaaag
     2041 ggcttgtccc gtcgcagcct ctggcgcgac ggcaggggga caatcaccgc gcggcgatgc
     2101 cgtgctgcga agcaaaaaag gatcgaatag gcgtcccaat ggccgaaacc gtgaaaaacc
     2161 agccgaagaa gcccgtcaag gctccggagc gcaaagcctt caaatatccc agcgaagacg
     2221 aagctttcgt gcggcggctg ggcagcgccg tgctggcgcg ctggagcgaa ctgcccgaag
     2281 agctgcgcgc cgtcattctc gccgaagcct gcacggtgtg ggac
//
LOCUS       DASWPU010000375         1947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_15469,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000375 DASWPU010000000
VERSION     DASWPU010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15469"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VGC27_08120"
     CDS             complement(<1..281)
                     /locus_tag="VGC27_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0282573.1"
                     /translation="MATHKKPGNWRPRTQAVRSGQTRTPFQETAEALFLTSGYAYDAP
                     EEAEARFKGEADGFTYTRFGNPTISMFEARMAALEGAPCARATASGMAAV"
     gene            435..845
                     /locus_tag="VGC27_08125"
     CDS             435..845
                     /locus_tag="VGC27_08125"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEY0282574.1"
                     /translation="MKTISHHAALVYVMVVVSAADGAMGDNELRAIGELTRTLPVFKD
                     FDRDRLLVLAQDCSAILQEDDGLAAVLGLVKEALPAPLRETAYWLALEVALSDTGVKL
                     EEARIIETLRRALGLDRLVAAALERGARVRHQIA"
     gene            842..1375
                     /locus_tag="VGC27_08130"
     CDS             842..1375
                     /locus_tag="VGC27_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0282575.1"
                     /translation="MKRLLLLRHAKAERGGHGAGDKLRALAPRGREDAARLGRFLNDE
                     AYIAGLVLCSASARTRETLELILPQIDPKPAVQYLDELYLAEAETILELMRGTREAIG
                     SLMIVGHNPGLEECARSLVRPPAERNLRKRYQMMAEKFPTGSLAVIDFQAERWRAIGG
                     WVGELEIFVRPKDLREE"
     gene            complement(1381..>1947)
                     /locus_tag="VGC27_08135"
     CDS             complement(1381..>1947)
                     /locus_tag="VGC27_08135"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0282576.1"
                     /translation="RSRKASCMGNLAIQVRPARPEDAAEIARVYIESWHDTYPGVLPS
                     SLLRAMTPKGQTARWQASIRAAGREAVLVAESGRYGIVGMASLGPARDDGLGLDGEIY
                     TLYVDPAFYGRGVGRSLLKGAFSALRKTGMSSCVIWAHTRNHARFFYEAMGGRLIAER
                     TAKLMGEAVPETAFGWKTLALAERSPAR"
BASE COUNT          320 a          627 c          654 g          346 t
ORIGIN      
        1 actgccgcca tgccgctggc cgtggcgcgg gcgcaaggcg cgccttcgag cgccgccatc
       61 cgcgcttcga acatcgagat cgtcggattt ccgaagcgcg tataggtgaa tccgtcggcc
      121 tcgcccttga aacgggcttc cgcctcttcg ggcgcgtcat aggcgtagcc tgaggtcagg
      181 aacagcgctt cggcggtctc ctgaaagggt gtgcgcgtct gcccgctgcg caccgcctgg
      241 gtgcgtggac gccaattgcc cggctttttg tgtgtcgcca ttctgagagg ttcgtatcgc
      301 ttgcgcttca ggtgacgcca attcgtcatt atgaggcgcc cgccgcaagc gggccgtgaa
      361 ccatgatgcg ccttgccggg ccgggtcaag tttcttaagg ttcgccccgc aattttgggc
      421 gccgatattc ggacatgaaa accatcagcc accacgcagc ccttgtgtac gtcatggtcg
      481 tcgtttccgc cgcggacggc gcgatgggcg acaacgagct gagggcgatc ggcgaactga
      541 cccgcacgct gccggtattc aaggatttcg accgcgaccg gctgctcgtc ctggcgcagg
      601 attgcagcgc catcttgcag gaggacgacg ggctggccgc ggttctcggc ctggtgaagg
      661 aggctttgcc cgcgccgttg cgcgagaccg cctattggct ggcgctcgaa gtcgcccttt
      721 ccgacacggg cgtaaagctg gaggaggcgc gcatcatcga gacgctgcgc cgcgctctcg
      781 gcctcgaccg tctcgtcgcc gctgccctgg agcgcggagc gcgggtgagg caccaaatcg
      841 cttgaaacgg cttctccttc ttcgtcacgc caaggcggag cgcggcggac acggcgccgg
      901 cgacaaactc cgggccctgg cgccgcgcgg ccgggaggat gcggcgcgcc tcggccgctt
      961 cctgaacgat gaggcttata ttgccggcct tgtgctgtgc tcggcctcgg caagaacgcg
     1021 cgagacgctg gaactcatcc tgccccagat agacccgaag ccggcggtgc agtatctcga
     1081 cgagctttat ctggcggaag cggagacgat tctcgagctg atgcgcggca cgcgcgaggc
     1141 gatcggctcg ctgatgatcg tcggccacaa tcccgggctg gaggaatgcg cgcggtctct
     1201 ggtccgccct ccggccgagc gaaatctccg caagcgctac cagatgatgg cggagaaatt
     1261 cccgaccggt tcgctcgcgg taatcgactt tcaggccgaa cgctggagag ccatcggcgg
     1321 ctgggtcgga gaactcgaga ttttcgtgcg gccgaaggat ctgcgcgaag agtaagaaat
     1381 tcaccgcgcc ggactgcgct cggcgagcgc cagcgtcttc cagccgaagg cggtttccgg
     1441 aaccgcttcg cccatcagct tggcggtgcg ttcggcgatc agcctgccgc ccatcgcctc
     1501 gtagaagaaa cgcgcatggt tgcgggtgtg cgcccaaatc acgcaggacg acattcccgt
     1561 cttgcgcagg gcggagaagg cgcctttgag cagcgaacgg ccgacgccgc ggccgtaaaa
     1621 agccggatcg acatagagcg tatagatttc gccgtccaaa ccgagcccgt cgtcgcgcgc
     1681 ggggcccaga cttgccatgc cgacgatgcc gtaacggccg ctttcggcca ccagcaccgc
     1741 ctcgcggccc gcggcgcgga tggaggcctg ccagcgcgcg gtctggcctt tcggcgtcat
     1801 ggcgcgcagc agcgagctgg gcagaacgcc cggataggtg tcgtgccagg attcgatata
     1861 gacgcgcgcg atctctgcgg catcttctgg ccgcgccggc ctgacctgta tggcgaggtt
     1921 gcccatgcat gaagctttgc gcgaacg
//
LOCUS       DASWPU010000376         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_34084,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000376 DASWPU010000000
VERSION     DASWPU010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34084"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VGC27_08140"
     CDS             <1..1102
                     /locus_tag="VGC27_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY0282577.1"
                     /translation="KAAMFETGEGIDWATAEALAFGTLLKEGVAVRLSGQDTARGTFS
                     QRHSVLTDQDTDERYVPLNHVGDDQPEFEVVDSLLSEEAVLGFEYGYSTAEPKALVLW
                     EAQFGDFANGAQVIIDQFIASGEMKWLRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAQDNLQVACPTTPANYFHVLRRQMHRSFRKPLILMTPKSLLRHKRCVSFLSDLEPGT
                     TFHRVFRDQAECVPGATTVKLVPDAEIKRVILCSGKVYFDLMEEREKKNESRVQMLRL
                     EQLYPFPDAVLARELERYPRAEVVWCQEEPKNQGAWTFVRPRMEEIMTLMSGHCACPR
                     YIGRPEYASTAAGLLSQHNAELHAFLDEALTL"
     gene            1207..>1692
                     /locus_tag="VGC27_08145"
     CDS             1207..>1692
                     /locus_tag="VGC27_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY0282578.1"
                     /translation="MSIQIKVPAMGESVTEATVARWFKKEGDAVARDEPLLELETDKV
                     TVEVPAPADGAIESIAVKAGDTVQVGSILGAIAEGKAGKATPVMSRNPDAVAPKTAPE
                     PEAIKLAPAVAKLAVAASAAAGVSPSVRRIAEESGLDPAHAKSTGKGGRVTKGDMLAT
                     LE"
BASE COUNT          325 a          548 c          541 g          278 t
ORIGIN      
        1 taaggccgcg atgttcgaaa ccggcgaagg catcgattgg gcgaccgccg aagcgctggc
       61 cttcggcacc ctgctcaagg aaggcgtcgc ggtgcgcctg tcgggacagg ataccgcgcg
      121 cggcaccttc tcccagcgcc actccgtgct caccgaccag gacaccgatg aacgctacgt
      181 gccgctcaat catgtgggcg acgaccaacc ggagttcgag gtcgtggact cgctgctttc
      241 cgaagaggcg gtgctgggct tcgagtacgg ctattcgacg gccgagccca aggcgttggt
      301 gctgtgggag gcgcagttcg gcgatttcgc caacggcgcc caggtcatca tcgaccagtt
      361 catcgcctcg ggcgagatga aatggctgcg catgtgcggg ctggtgatgc tgctgccgca
      421 cggctacgaa ggccagggac cggagcattc ctcggcgcgg ctggagcgct atctccagct
      481 ttgcgcccag gacaatctgc aagtcgcctg cccgaccacg ccggcgaatt acttccacgt
      541 gctgcggcgg cagatgcatc gcagcttccg caagccgctg atcctgatga cgccgaaatc
      601 gctgctgcgc cataagcgct gcgtctcgtt cctgtcggat ttggaaccgg ggacgacgtt
      661 ccaccgcgtc ttccgtgacc aggccgaatg cgtgcccggc gccaccacgg tcaagcttgt
      721 ccccgacgca gagatcaagc gcgtgatcct gtgcagcggc aaagtctatt tcgacctgat
      781 ggaagagcgc gagaagaaga acgaaagccg cgtccagatg ctgcggctgg agcagctcta
      841 tcccttcccc gacgccgtgc tggccagaga actcgaacgc taccccaggg cggaggttgt
      901 ctggtgccag gaagagccca agaaccaggg cgcctggacc ttcgtgcggc cgcggatgga
      961 agagatcatg accctgatgt ccggccactg tgcctgtccg cgctatatcg gacggccgga
     1021 atacgcctcc accgccgccg gcctgttgag ccagcataat gccgaacttc acgccttcct
     1081 cgacgaggct ttgacgctgt aaattcgaat cgtttccctt acccttcgac aagctcaggg
     1141 tgagggtgtt tctaatgttc ccctcatgct gagcctgtcg aagcatgaag tccgaaagag
     1201 gctccgatga gcatccagat caaggttccg gcgatgggcg aatccgtcac cgaagcgacc
     1261 gtcgcccgct ggttcaagaa ggaaggcgat gcggtggccc gcgacgagcc gctgctggaa
     1321 ctggaaaccg acaaggtcac ggtggaggtt cccgccccgg ccgacggcgc gatcgagagc
     1381 atcgcggtga aagcgggcga cacggtgcag gtcggcagca tcctgggcgc catcgccgaa
     1441 ggcaaagccg gcaaagccac gccggtgatg tcgcgcaatc ccgacgccgt tgcgcccaag
     1501 accgcacccg aaccggaagc catcaagctt gcgcccgccg tcgccaaact cgcggtcgcg
     1561 gcctctgcgg cggccggcgt ttcgccgtcc gtgcggcgga tcgccgagga aagcggcctc
     1621 gatcccgcgc atgcaaaaag caccggcaag ggcggccgcg tcaccaaagg cgacatgctg
     1681 gctaccctgg aa
//
LOCUS       DASWPU010000377         4683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_8909, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000377 DASWPU010000000
VERSION     DASWPU010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8909"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1762)
                     /locus_tag="VGC27_08150"
     CDS             complement(<1..1762)
                     /locus_tag="VGC27_08150"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY0282579.1"
                     /translation="MGADPIPALRRFAPDPLFTAGALAEACARLFAAVRRQAAADRMR
                     WPLWLPVAMAAGIGLYFALPEEPVWGVAVIAAGVALAAGTLSAATVTLPRIALALLAA
                     ASLGFAAAKLRTELVRAPVLARKLGPVKFDARVVESEPRGKGSRMVLEPVRIGRLKPE
                     QTPHFVRLSVRARSAVPEPGSWLRVLAVLQPPPAPSMPGDYDFGRGAYYRRIGAVGYL
                     YGRPKPIAPVRAATRLEELGAGLERLRGRMTARVRSIVPGNEGAVAAALITGERVDID
                     SDVQADFRDSGLMHVLSISGLHLALAGGFFFWTMRALLALFPAIALRFPVKKWAALAA
                     VAGATFYLLVSGCEAPAVRSYIMLTMMFLAVLFDRPALSMRAVALAAAIIMLFAPESV
                     IGPGFEMSFAAVIGLIALAEWEQARRAAKPESGQKTPVTRIARYAWGIAVTSIVATLA
                     TTPIAIFHFDRSTQYGLLANLAALPVVGAVIMPAATVAMVLMPFGLDEWPLIVMGKGV
                     GVMNAIAHWVAALPGAATVVPVWPLWCLIAVLGGGLWIAFWRMPWRWLGLLPISIGIA
                     FAFGTTQPDILVARDVRTVAV"
     gene            1831..3237
                     /gene="gltX"
                     /locus_tag="VGC27_08155"
     CDS             1831..3237
                     /gene="gltX"
                     /locus_tag="VGC27_08155"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY0282580.1"
                     /translation="MTENQKPVLRFAPSPTGMLHIGGARTALFNWLYARHTGGKFLLR
                     IEDTDRERSTPEAVGAILNGLTWLGLNWDGETVYQFKRAGRHREVAERLLAEGKAYRC
                     YATPQELEEMRAAQRAAGKPIRYDGRWRDRDPGEAPAGAPFAVRLKARQSGETIVHDL
                     VQGDVRFAHEQLDDMVLLRGDGNPTYMLAVVVDDYDMGITHIIRGDDHLNNAARQLQI
                     IEALGWPEPVYAHLPLIHGPDGAKLSKRHGALAVEAYRDMGYLPETMRNYLLRLGWSH
                     GDDEIIPTAQAIEWFSLENIGRSPARLDFKKLDNLNAHYIRAMDDKVLVDEIAAVLAR
                     KNTPLSEKARARLIAAMPNLKERAKTLVELAGAAEFLCADGARTLDEAAQKILSEEAR
                     RLNGEFLALTAASPWEAAALEEKARSFAEAKGLKLGQVAQPLRAAVTGKTTSPPIFAM
                     LEVLGRDEATARIKAYSA"
     gene            3344..4654
                     /gene="gltA"
                     /locus_tag="VGC27_08160"
     CDS             3344..4654
                     /gene="gltA"
                     /locus_tag="VGC27_08160"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY0282581.1"
                     /translation="MMRESKAVPAGKATLSYQGKSYEFPAFAGSEGPNVIDIRKLYGE
                     ADLFTYDPSFTSTASCESDITFIDGDKGILLHRGYPIEQLAEKSSFLEVCYLLLYGEL
                     PTRQQMMEFDRTITRHTMVHEQLASFFRGFRRDAHPMAVMCGVVGALSAFYHDSTDVN
                     DPKQRQTASHRLVAKMPTIAAMAYRYSTGHPFVYPLNKLGYTENFLRMCFSVPAEDYV
                     VNPVLAKALDTVFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGAN
                     EAALKMLGEIGTVERIPEFIKRAKDKNDPFRLMGFGHRVYKNYDPRAKVMQKQCHAVL
                     DELGLHNTPLLKVALELERIALQDEYFVEKKLYPNIDFYSGITLRALGFPVSMFTALF
                     ALARTVGWIAQWKEMLEDPHQKIGRPRQIYTGSPHRDYVPVAKR"
BASE COUNT          952 a         1566 c         1460 g          705 t
ORIGIN      
        1 tgacggcgac tgtgcgcacg tcgcgcgcca ccagaatatc cggctgcgtg gtgccgaagg
       61 caaacgcgat cccgattgaa atcggcagca gtcccagcca tctccaaggc atgcgccaga
      121 acgcgatcca caagccgccg cccagcaccg cgatcagaca ccagagcggc cataccggca
      181 cgaccgtggc cgcgccgggc agcgccgcca cccaatgggc gatggcgttc atcacgccga
      241 cgcccttgcc catgacgatc agcggccatt cgtccaatcc gaacggcatc aggaccatgg
      301 ccacggtcgc cgccggcatg atcacggcgc cgacgaccgg caaagcggcc agattggcaa
      361 gcaggccgta ctgcgtcgag cggtcgaaat ggaagatggc gatgggcgtg gtcgcgagcg
      421 tcgcgacgat gctggtaacg gcgatgcccc aggcatatcg cgcgatgcgc gtgacgggcg
      481 ttttctgccc gctctcgggt ttcgcggcgc ggcgagcctg ttcccattcg gccagcgcga
      541 tcaatccgat caccgcggcg aacgacattt cgaagcccgg cccgatcacg ctttcgggcg
      601 cgaacagcat gatgatcgcg gcggcaagcg ccaccgcccg catcgacagc gccgggcggt
      661 cgaacagcac cgccaggaac atcatcgtca gcatgatgta ggagcgcacc gcgggcgctt
      721 cgcagccgct gaccagaaga taaaaggtgg cgcccgccac cgccgccagc gcggcccatt
      781 tcttgaccgg aaagcgcagc gcgatcgccg ggaacagcgc caacagcgcc cgcatcgtcc
      841 agaagaagaa gccgcccgcc agcgccagat gcagccccga aatcgacagc acatgcatca
      901 gcccggaatc gcggaagtcg gcctggacgt cggaatcgat atcaacccgc tcgccagtga
      961 tgagagcggc ggcaacggcg ccctcgttgc cgggaacgat gctccgcacc cgcgccgtca
     1021 tgcggccgcg cagacgttcg agcccggcgc caagctcctc caaacgggtc gctgcccgca
     1081 ccggcgcgat gggcttcggc cggccataga gatagcccac ggcgccgatg cggcggtaat
     1141 aggctccgcg gccgaaatca tagtcgccgg gcatggaagg tgccggcggc ggctggagta
     1201 cggccagcac gcgcagccag cttcccggct ccggcacggc gcttctcgcc cgcaccgaga
     1261 ggcggacgaa gtgcggcgtc tgttccggtt tgagccggcc gatgcggacg ggctccagca
     1321 ccatgcggct gcccttgccg cgcggttcgg attcgaccac gcgcgcatcg aacttcaccg
     1381 gtcccagctt gcgggcgaga acgggcgcac ggacaagttc ggtccgcagc ttcgccgcgg
     1441 caaagccgag agaggccgcc gccagcagcg ccaaggcgat acgagggagc gtcaccgtcg
     1501 ccgccgacaa cgtcccggct gcaagcgcca ctccggccgc tatgacggca acgccccaga
     1561 ccggctcttc gggcagcgca aagtacaagc cgatacccgc tgccatcgcc acggggagcc
     1621 acaagggcca gcgcatgcgg tcagcggcgg cctgcctgcg gacagcggca aacaggcgcg
     1681 cgcaagcctc cgccagcgcc cctgctgtga agagcggatc gggcgcaaag cgccgcaacg
     1741 ccgggatcgg atctgccccc acggacgaat gccgtttgcc agaagcctcg gtttcactta
     1801 aagccgaaga aacgccagcc gaagtccatc atgaccgaaa accagaagcc agtcctgcgc
     1861 tttgcgccct cgcccaccgg aatgttgcat atcggcggcg cccgcacggc gttgttcaac
     1921 tggctctatg cccgccacac gggcggcaag ttcctgctgc gtatcgagga caccgaccgc
     1981 gaacgctcca cccccgaggc cgtgggtgcc atcctgaacg ggctgacttg gctggggctg
     2041 aattgggatg gtgagaccgt ctatcaattc aagcgggccg gccgccaccg cgaggtcgct
     2101 gagcggctcc tcgccgaagg caaagcctat cgctgttacg ccacgcctca ggagctggaa
     2161 gagatgcgcg ccgcccagcg cgcggccgga aagcccatcc gctacgacgg gcgctggcgc
     2221 gaccgcgacc ctggcgaagc gcctgcgggc gcaccgttcg cggtgcggct gaaggcaagg
     2281 cagagcgggg aaaccatcgt gcacgacctg gtgcagggcg acgtgcgctt cgcccacgaa
     2341 cagctcgatg acatggtgct gctgcgcggc gacggcaacc cgacctacat gctcgccgtc
     2401 gtcgtggacg attacgacat gggcataacc cacatcatcc gcggcgacga tcatctcaac
     2461 aacgccgccc gccagctcca aatcatcgag gcgctgggct ggccggaacc ggtttacgcc
     2521 catctgccgc tgatccacgg acccgacggc gccaagctgt ccaagcgtca cggggccttg
     2581 gccgtcgaag cctatcgcga catgggctat ctgcccgaga cgatgcgcaa ctatttgctg
     2641 cggctgggct ggagccacgg cgacgacgag atcatcccga cggcacaggc catcgagtgg
     2701 ttcagtcttg agaacatcgg ccgaagcccg gcgcggctgg atttcaagaa actcgacaat
     2761 ctcaacgccc actacatccg cgccatggac gacaaagtgc tggtggatga aatcgccgcc
     2821 gtgctggcgc gaaagaatac gccgttgagc gagaaagccc gcgcccggct gatcgccgcc
     2881 atgccgaacc tgaaggagcg cgccaagacc ttggtggaac tggccggcgc cgcggagttc
     2941 ctgtgcgcgg acggcgcacg cacgctggac gaagcggcgc agaagattct cagcgaagag
     3001 gcgcgccgcc tgaacggcga attccttgcg ctcacggcag catcgccgtg ggaagcggcg
     3061 gcgctggaag aaaaagcccg ctctttcgca gaagcgaaag gactcaagct gggccaagtc
     3121 gcacaacctt tgcgggcggc cgtgaccgga aaaacgactt cgcctccgat ttttgccatg
     3181 ctggaagtac ttggaaggga cgaagcgacc gccaggatca aagcctatag tgcctagttc
     3241 gtgggcaaat taccgatctg catagaaaag ttaggtgacg aagtcgattc gggccatcta
     3301 taactgctgc aatgcaagat aaacaagcca ggagccagga aacatgatgc gagagagcaa
     3361 agccgtcccc gctggcaagg cgacactcag ctaccagggc aaatcctacg agtttccggc
     3421 ctttgcgggc agcgaaggcc cgaacgtcat cgacatccgc aaactctatg gcgaggccga
     3481 tctcttcact tacgatccca gcttcacttc cacggcgagt tgcgaaagcg acatcacctt
     3541 tatcgacggc gacaagggca ttctgcttca tcgcggctat ccgatcgagc agttggccga
     3601 gaaatcgtcg ttcctcgaag tctgctacct gctgctctac ggcgaattgc cgaccagaca
     3661 gcagatgatg gaattcgacc gcaccatcac gcggcacacg atggtgcatg agcaattggc
     3721 ttcgttcttt cgcggcttcc gccgcgacgc gcacccgatg gcggtgatgt gcggcgtggt
     3781 gggggcgctg tcggcgttct atcacgactc cacagacgtc aacgatccga aacagcgcca
     3841 gaccgccagc caccgcctcg tcgccaagat gccgaccatc gcggccatgg cttaccgcta
     3901 ctcgaccggt catcccttcg tctatccgct gaacaagctc ggctacaccg agaatttcct
     3961 gcgcatgtgc ttctcggttc cggcagagga ttacgtggtc aatccggtgc tggccaaggc
     4021 gctggacacg gtcttcatcc tgcacgccga tcacgagcag aatgcttcca cctccacggt
     4081 gcggctggcc gggtcctccg gcgccaatcc gttcgcctgc atcgcggcgg gcatcgcctg
     4141 cctgtggggc ccggcgcacg gcggcgccaa cgaagcggcg ctgaagatgc tgggagagat
     4201 cggaaccgtc gagcgcatcc ccgaattcat caaacgcgcc aaggataaga acgatccctt
     4261 ccgcctgatg ggcttcggcc atcgcgtcta caagaattac gatccccgcg cgaaagtgat
     4321 gcagaagcaa tgccacgccg ttctcgacga gcttggcctg cacaacaccc cgctcttgaa
     4381 agtggcgctg gaactggagc gcatcgcgct gcaggacgag tatttcgtcg aaaagaaact
     4441 ctatcccaac atcgatttct attccggcat cacgctgcgc gcgttgggct ttcccgtcag
     4501 catgttcacg gcgctgttcg cgctggcgcg caccgtcggc tggatcgcgc agtggaagga
     4561 gatgctggag gacccgcacc agaagatcgg acggccgcgg cagatctaca ccggcagccc
     4621 gcaccgcgac tacgtgccgg tggcgaagcg gtaggtccat tcgccataaa atcggtgtca
     4681 tgg
//
LOCUS       DASWPU010000378         5588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_15470,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000378 DASWPU010000000
VERSION     DASWPU010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15470"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VGC27_08165"
     CDS             <1..920
                     /locus_tag="VGC27_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0282582.1"
                     /translation="HADMSRSRLRQLIDGGAVTRGGETIRDPNHRVKPGEIYLIEVPP
                     TVPAEPQGQDIPLAVVYEDADLIVIDKPAGLVVHPAAGNPDGTLVNALIAHCGASLAG
                     VGGVARPGIVHRLDKDTTGLLVAAKNERAMKSLAKQFANHTIERAYHAVVWGSPRLGD
                     GLIETQIGRNPFDRKRMGVLRGGGKEARTRYRVIERFGSETRPLASLIECRLETGRTH
                     QIRVHLTHLGHPLIGDASYGRSRQAPRAKSEAEAAAYEAIANFPRQALHAYLLGFQHP
                     SLHKTLRFESKWPADLSGLIAALHDLKNP"
     gene            1099..2001
                     /gene="rpoH"
                     /locus_tag="VGC27_08170"
     CDS             1099..2001
                     /gene="rpoH"
                     /locus_tag="VGC27_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY0282583.1"
                     /translation="MSSRALPALHSEGGLSRYLAEIRKFPLLAPEEEYMLAKRWKEHE
                     DANAARKLITSHLRLVAKIAMGYRGYGLPVSEIVAEGNVGLMQAVKRFDPERGFRLAT
                     YAMWWIRASIQEFILRSWSLVKIGTTSDQKKLFFNLRKAKSKINAIEEGDLSQAHVTK
                     LADQLGVSEREVVNMNRRLSAPDSSLNAPLRGDGESEWQDWLADDSLDQETVMAESEE
                     VAERRELLDAALGELPEREREIIRARRLQDEPATLEELSHRFGISRERVRQIEVRAFD
                     RLQRSMKNALREKRSATLNMNAPE"
     gene            2130..2942
                     /locus_tag="VGC27_08175"
     CDS             2130..2942
                     /locus_tag="VGC27_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY0282584.1"
                     /translation="MNLKLWQIDAFADKPLEGNPAAVVPLETWIDAGLMQKIATENNL
                     SETAFFVKTAPGRYDLRWFTPASEVELCGHATLASAWLVFETFDPELTAVTFQTRSGE
                     LIVTRGRDGRHLMSLPSDNVAPFEAPAGFARDLGEALGVAPPKEILRSRYLIAVWSEA
                     KAVRAIKGPDGVARVLRLIGMWGLIATAKGDAGYDFVSRFFAPDKGVPEDPVTGSAHC
                     ALTPFWAKRLSKKTLRARQVSPRGGDLTVTDEGVRAVISGACALYMKGEIVV"
     gene            2975..3925
                     /locus_tag="VGC27_08180"
     CDS             2975..3925
                     /locus_tag="VGC27_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0282585.1"
                     /translation="MTIANALRHILPALCAGVLLAAAAQGAPAQARTQAPGYFRYADG
                     AFEVTALYDGYFYIDTKILHGIEPKAIAPLLAASFTGNSKGILTSVNAFLVHTGDHLV
                     LVDAGMAGCPSATLGHMLENFRAAGYRPEDVDTVLITHMHNDHVCGLAADGKSVFPNA
                     TVYAAQEEAAFWLDDAKQKSFERVRAALAPYREANKFRTFKGGDAVLPGVTAIATHGH
                     TPGHTSYLFQSGPRSLVVLGDIVHVAALQFARPGVSIDYDTNPKEAVAERRDLFAELA
                     RTGWTAAGAHLPFHGIGHVRKDGKAYAYVPVEYAPLPDKP"
     gene            3991..4329
                     /locus_tag="VGC27_08185"
     CDS             3991..4329
                     /locus_tag="VGC27_08185"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0282586.1"
                     /translation="MSEAALADPILVRLKRELVALYGPRLKQALLYGSRARGDFREDS
                     DYDVLVVLEGPLDDWKELDRLAQLSSDITWDTVATGRPIVASFKAVTEDMVRQRTGFM
                     HNVRREGIPI"
     gene            4326..4703
                     /locus_tag="VGC27_08190"
     CDS             4326..4703
                     /locus_tag="VGC27_08190"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEY0282587.1"
                     /translation="MSPEAVDYMSVAEAALDRARRAFLAEIYEDAARNAYTSALNAAR
                     AIIFDKTGLAVKSHSGTRTKLHELVWKGLAFDKSLVDFLARAFELRQRVEYGPPTFVQ
                     RAEADEYLERAAAFLAAAKKVCA"
     gene            4840..>5588
                     /locus_tag="VGC27_08195"
     CDS             4840..>5588
                     /locus_tag="VGC27_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEY0282588.1"
                     /translation="MTGTVPAVRPARPFFSSGPCAKRPGWTPETLNAALLGRSHRSKP
                     GKAKLKDAIDRTKALLGIPADYRVGIVPASDTGAVEMAMWSLLGPKPVDLFAWESFGE
                     DWVTDTVKQLKLDSRVFKAAYGELPDLKQADPKHDIVFLWNGTTSGVRVPDGDWISDA
                     REGLTICDATSAVFAMDIPWQKLDVATFSWQKVLGGEAAHGILILSPRVVARLETYTP
                     PWPMPKIFRMTKAGKLVEGIFEGETINTPSML"
BASE COUNT         1045 a         1793 c         1766 g          984 t
ORIGIN      
        1 cgcacgccga tatgagccgc tcgcgcctgc gccaactgat cgacgggggc gcggtgacgc
       61 gcggcggcga aacgataaga gacccgaacc acagggtcaa accgggcgaa atctacctga
      121 tcgaggtgcc gccgaccgtc cccgccgagc cgcagggcca ggacatcccc cttgccgtgg
      181 tttacgagga cgcggacctg atcgtcatcg acaagccggc ggggctggtg gtccatccgg
      241 cggcgggaaa tcccgacggc accctggtca acgccctgat cgcccattgc ggcgcctcgc
      301 tggcgggggt gggcggggtg gcgcgtccgg gcatcgtcca tcgcctggac aaggatacca
      361 ccggcctctt ggtcgcggcc aagaacgagc gcgccatgaa gagcctggcc aagcagttcg
      421 ccaaccacac catcgagcgc gcctatcacg ccgtcgtatg gggcagcccg cggctcggcg
      481 acggcctgat cgagacccag atcggccgca atcccttcga ccgcaaacgc atgggggttt
      541 tgcgcggcgg cggcaaggaa gcccgcacgc gctatcgcgt gatcgaacgt ttcggcagcg
      601 agacgcgccc cctcgcctcg ctgatcgaat gccggctgga aaccgggcgc acgcatcaaa
      661 tccgcgtgca tctgactcac ctagggcacc ctctgatcgg cgatgcctcc tatgggcgtt
      721 cgcgccaggc gccgcgggcg aagagcgaag ccgaagcggc ggcctatgaa gccattgcaa
      781 actttccgcg acaggcgctg cacgcctatt tattgggctt ccagcacccc agcctgcaca
      841 aaaccctgcg tttcgagtcg aaatggccgg cggacttatc cggcctgatt gccgccctgc
      901 atgacttgaa aaatccttaa tcgctcctac atcttgcctc gttttcaagg tgtatttccg
      961 ttcactttat cccgccctgc aaggagcgta gtaaagtgta agtccggcgc gcttgggtgc
     1021 cggatctttt tggttgtcgc gcaatctcac ggcgaaccgc tacacacttc gcctgattgc
     1081 gctttatggg gttcgacaat gagcagccgt gcgcttccgg ccctccacag cgaaggcggt
     1141 ctatcgcgct accttgccga aatccggaag ttcccgcttc tggcaccgga agaagagtac
     1201 atgctcgcca agcgctggaa ggagcatgaa gacgccaacg ccgcgcgcaa gctcatcact
     1261 tcgcatctgc gcctggttgc caagatcgcc atgggctatc gcggctacgg cttgccggtg
     1321 tcggagatcg tggccgaggg caatgtcggg ctgatgcagg cggtcaagcg cttcgatccc
     1381 gagcgcggct tccgcctggc gacctatgcg atgtggtgga ttcgcgcctc gatccaggaa
     1441 ttcatcttgc gctcctggag cctggtgaag atcggcacca cctccgacca gaagaagctg
     1501 ttcttcaacc tgcgcaaggc caagagcaag atcaacgcca tcgaggaagg cgatctaagc
     1561 caggcgcatg tgaccaagct cgccgaccag ctcggcgttt cggaacgcga agtcgtcaac
     1621 atgaaccggc ggctgtcggc gccggactcc tcgctcaacg cgccgctgcg cggggacggc
     1681 gagtcggaat ggcaggattg gctggccgac gattccctcg accaggaaac cgtgatggcc
     1741 gagtccgaag aagtcgccga gcggcgcgag ctgctcgacg cggcgctggg cgaactgccc
     1801 gaacgcgagc gcgagatcat ccgcgcgcgc aggctgcagg acgagccggc gacgctggag
     1861 gaactctccc accgcttcgg catcagccgc gagcgggtgc gccagatcga agtgcgcgcc
     1921 ttcgaccgct tgcagcggag catgaagaac gcgctgcgcg agaaacgcag cgcgacgctg
     1981 aacatgaacg cgccggagta gcgcgccgct tcccgaaaac cgccgccatc ccggttatgc
     2041 cggttgatcg taaccgttat gcactcgcgc gtgttcgcga tgcgcgcctc tcgcgttagc
     2101 gttcgagatc ccgacggaaa ggcgatgcga tgaacctcaa gctctggcag atcgatgcgt
     2161 tcgcggacaa gccgctggaa ggcaacccgg ccgcggtggt gccgctcgag acctggatcg
     2221 acgccggact gatgcagaag atcgcaaccg aaaacaacct ctccgagacc gcgttcttcg
     2281 tgaagaccgc gccggggcgt tacgatctgc gctggttcac gccggcttcg gaagtcgagc
     2341 tgtgcggaca cgcgacgctg gccagcgcct ggctggtgtt cgagacgttc gatcccgaat
     2401 tgacggccgt gaccttccag acccgctccg gcgagctgat cgtaacgcgc gggcgcgacg
     2461 ggcggcacct gatgtcgctg ccgtcggaca atgtcgcgcc gttcgaggcg cccgccggat
     2521 tcgcgcgcga cctcggcgaa gcgctggggg tggcgcctcc caaagagatt ctcagaagcc
     2581 gctatctcat cgcggtctgg agcgaggcga aggcggtgcg cgcgatcaag ggcccggacg
     2641 gcgtcgcgcg cgtgctgcgc ctgatcggca tgtggggact gatcgccacc gccaagggcg
     2701 atgcaggcta cgacttcgtt tcccgcttct tcgcgcccga caaaggcgtg ccggaagatc
     2761 cggtaaccgg ctcggcccat tgcgcgctga cgccgttctg ggccaagcgg ctgtccaaga
     2821 agaccctgcg cgcgcgccag gtttcgccgc gcggcggcga tcttaccgtc accgacgagg
     2881 gcgtccgcgc ggtgatttcc ggcgcctgtg cgctttatat gaaaggcgaa attgtggtct
     2941 agtgacgccg cacaccgatg aacggggaga cgtcatgacc atcgcgaacg ccttgcgcca
     3001 tatcctgcct gcgctttgcg cgggcgtgct tctcgccgcc gccgcccagg gcgcgcccgc
     3061 gcaggcgaga acccaagcgc cgggttattt ccgctatgcc gacggcgctt tcgaggtgac
     3121 cgcgctgtac gacggttatt tctatataga caccaagatt ctccacggca tcgaaccgaa
     3181 ggccatcgcc ccgctgctcg ccgcgtcgtt taccggcaac agcaaaggca ttctgacctc
     3241 ggtgaacgcc ttcctcgttc acaccggcga tcatctggtg ctggtcgatg ccggcatggc
     3301 gggatgcccc tccgccacgc tggggcacat gctggaaaac ttccgcgccg ccggctaccg
     3361 gcccgaagac gtggacaccg tgctcatcac ccacatgcat aacgatcacg tctgcggtct
     3421 cgccgccgac ggcaagagcg tctttcccaa cgccaccgtg tatgccgcgc aggaagaagc
     3481 cgcgttctgg ctggacgacg caaaacaaaa gagcttcgag cgggtacgcg cggcgctggc
     3541 gccttaccgg gaagcgaaca aattccgcac gttcaagggc ggcgacgcgg tgcttccggg
     3601 cgtgaccgcc attgccacgc acggccatac gccggggcat acgtcgtacc tgttccaatc
     3661 cggaccccgg agccttgtgg ttctgggcga catcgtgcac gtcgccgcgc tgcaattcgc
     3721 ccgtcccggc gtttcgatag attacgatac caatccgaaa gaggccgtgg cggagcgccg
     3781 ggatttgttt gccgaactgg cccggaccgg atggacggcg gcgggagcgc atctgccctt
     3841 ccacggcatc ggccatgtgc gtaaggacgg caaggcttac gcctatgtgc cggtcgaata
     3901 cgccccgttg ccggacaagc cttagacagc agcgcaatcg aggttcgagg cgtcgttcct
     3961 gcaatcgcgg ttttgcggta tattgccgcc atgagcgaag cagcccttgc cgatccgatc
     4021 ctcgtccgcc tcaagcggga gcttgtggcg ctgtacgggc cgcgcctgaa acaggcgctg
     4081 ctctacggct cgcgcgcgcg cggcgatttt cgcgaggaca gcgattacga cgtgctggtg
     4141 gtgctggagg ggccgctgga tgattggaaa gaactcgacc gccttgcgca gctttcgtca
     4201 gacataacct gggatactgt tgccaccgga cgtccaattg tcgcgtcctt caaagcggta
     4261 acggaggata tggtcagaca gcggaccggg ttcatgcaca atgtacggcg ggaagggatt
     4321 ccaatatgag tcccgaagct gtcgactaca tgtccgtcgc ggaggcggct cttgatcgag
     4381 ctcgccgtgc ttttctggct gaaatctatg aggacgctgc ccgtaacgct tacacttcgg
     4441 cgttgaatgc agcgcgcgcg atcatattcg acaaaaccgg actcgcggta aagagccatt
     4501 ccggcacgcg tacgaagctg catgaattag tctggaaagg cttggcgttc gacaagagcc
     4561 tggtggattt tctcgccagg gcttttgagt tgaggcagcg cgtcgagtac ggcccaccaa
     4621 cttttgtgca acgtgccgag gccgatgagt atcttgagcg cgccgccgcc ttcctagccg
     4681 cggccaagaa ggtgtgcgct tagcgccaca cggaaacggc gttttgagca agaacagaaa
     4741 gccgcacatt tcccgtttga ctgtgtccgc gcgaatctat acatcggtca gcgggccacg
     4801 cctccccaac gaggcgcaac attctgagag gaatgtgaga tgaccggtac tgtaccggcc
     4861 gtgcgccccg cacggccttt tttttcgtcc ggaccttgcg ccaagcggcc gggctggact
     4921 cctgaaaccc tgaacgccgc cctgctcggc cgttcgcatc gctccaaacc cggcaaggcg
     4981 aagctgaaag acgccatcga ccgcaccaag gcgctgctcg gcattcccgc cgattatcgc
     5041 gtcggcatcg tgccggcatc cgacaccggc gccgtggaaa tggcgatgtg gtcgctgctg
     5101 gggccgaaac ccgtggacct gttcgcctgg gaaagcttcg gtgaagactg ggtcaccgat
     5161 acggtcaagc agctcaagct ggacagccgc gttttcaagg cggcttacgg cgagttgccc
     5221 gacctcaaac aggccgatcc taagcacgat atcgtgttcc tgtggaacgg cacgacctcg
     5281 ggcgtgcggg tgcccgacgg cgactggatc agcgacgccc gcgaaggcct cacgatctgc
     5341 gacgccacct cggcggtctt cgccatggat attccgtggc agaaactcga tgtcgcgacc
     5401 ttctcctggc agaaagtgct gggcggcgaa gcggcgcacg gcatattgat cctttccccg
     5461 cgcgttgtgg cgcggcttga aacctacacg ccgccttggc cgatgccgaa aatcttccgc
     5521 atgaccaagg ccggcaagct ggtggaagga atcttcgaag gcgaaaccat caacacgccg
     5581 tcgatgct
//
LOCUS       DASWPU010000379         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_3001, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000379 DASWPU010000000
VERSION     DASWPU010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3001"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VGC27_08200"
     CDS             complement(<1..593)
                     /locus_tag="VGC27_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282589.1"
                     /translation="MAGWVPQPKTACGQDAARLRWLFLDLNAYFASVEQQENPKLRGK
                     PIIVTPVASEGTCAIAASYEAKAFGIRTGTPVYEARRMCPGLKIVNARHDVYVDYHHR
                     VVDEIERHLHVTKVESIDEVACELLGAERRIENATALAREVQRGVLKRVGECLRASVG
                     LGPSVLLAKTASDMKKPAGLTVLGADQLPQAVLRLKLG"
     gene            complement(657..>1775)
                     /locus_tag="VGC27_08205"
     CDS             complement(657..>1775)
                     /locus_tag="VGC27_08205"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY0282590.1"
                     /translation="GGQFTANTMACVAEAIGLALPYSSGPPAEVLARDDFALKAGQAV
                     MALIEKRIRPRDICTRAAFENAAAVVAATGGSTNATLHLPALANEAGIKFDLFDVAAI
                     FKRTPYLASMKPGGKYVAKDMWEAGGVPMLMRALLDGGYIDGSCLTVTGKTVAENLKD
                     VVFNPDQKVMVDTAHALAPSGGLVGLRGNLAPEGAVVKIAGLKHLTHRGPARVFDCEE
                     DCFAAVQRRDYKEGDVLVIRYEGPKGGPGMREMLSTTAALYGQGVENIALITDGRFSG
                     GTRGLCVGHVGPEAAEGGPIGLLKDGDVIVIDAETGAITVELSDAELAARKAAWKPKA
                     PAFGSGALYRYSKNVGPARYGAITTPGAASEVRCYADI"
BASE COUNT          309 a          598 c          555 g          313 t
ORIGIN      
        1 ccgagcttga ggcggaggac cgcctgcggg agctggtcgg cgccgagcac cgtgaggccg
       61 gcgggctttt tcatgtcgct cgccgtcttg gcgagcagca cactggggcc caaacccacc
      121 gaggcgcgca agcattcacc gacgcgcttc aagacgcccc gctgcacctc gcgggcgagg
      181 gctgtggcat tttcgatgcg ccgttcggcg cccagaagct cgcaagccac ttcgtcgatg
      241 gattccacct tggtgacgtg cagatggcgc tcgatctcgt ccacgacgcg gtgatggtag
      301 tcgacataaa cgtcgtgccg cgcattgacg atcttgagac ctggacacat ccgccgcgct
      361 tcgtagaccg gcgtgccggt gcggatgccg aaggccttgg cctcgtagct ggcggcgatg
      421 gcacaagtcc cttccgaagc aaccggcgtg acgatgatgg gcttgccgcg cagcttggga
      481 ttttcctgct gctcgacgct ggcgaaatac gcgttcaaat cgaggaacag ccagcgcagg
      541 cgggctgcat cttgcccaca cgccgttttc ggttgcggaa cccatcctgc catgataaga
      601 acataacagg aacataaggg gcaaacaact aagcagaccc ggtcaaaaac gccgcatcaa
      661 atatcggcgt agcacctcac ctcgctcgcc gcgccgggcg tggtgatggc gccgtagcgc
      721 gccggtccca cattcttgga atagcgatag agagcgccgg atccaaaagc cggcgctttc
      781 ggtttccacg ccgctttgcg cgcggcgagt tccgcatcgg aaagctccac cgtgatcgcg
      841 ccggtttccg cgtcgatgac gatgacgtcg ccgtctttga gcaagccgat ggggccgccc
      901 tcggccgctt ccggcccgac atggccgacg cacagcccgc gcgtgccgcc ggagaatcgt
      961 ccgtcggtga tcagcgcgat attctccacg ccctgcccgt acagcgccgc cgtcgtggac
     1021 agcatttcgc gcattcccgg cccgcccttc ggcccttcgt agcggatcac cagcacgtcg
     1081 ccttccttgt agtcgcggcg ctgcacggcg gcaaaacaat cctcttcgca atcgaagacg
     1141 cgcgccggcc cgcggtgggt aaggtgcttg aggccggcga tcttcaccac cgcaccttcg
     1201 ggagcgagat tgccgcgcaa tcccaccaag ccgccggacg gcgccaaggc atgggccgta
     1261 tccaccatca ccttctgatc ggggttgaag accacgtctt tcagattttc ggccaccgtc
     1321 ttgccggtga cggtcaggca cgacccgtcg atatagccgc cgtccaagag cgcgcgcatc
     1381 agcatgggca cgccgccggc ttcccacata tccttggcga cgtatttgcc gcccggtttc
     1441 atcgaagcga gatagggcgt gcgtttgaag attgccgcca cgtcgaagag atcgaacttg
     1501 atccccgcct cgttcgccag cgcgggcagg tgcagcgtgg cgttggtgga accgcctgtg
     1561 gccgccacca ccgccgccgc gttctcgaag gcggcgcggg tgcagatgtc gcgcgggcgg
     1621 atgcgcttct cgatcaacgc catcaccgcc tgcccggcct tcagcgcgaa atcgtcgcgc
     1681 gccagtactt ccgccggcgg accggaggaa tagggcagcg ccagaccgat ggcttcggcc
     1741 acgcaggcca tggtgtttgc ggtgaactgt ccgcc
//
LOCUS       DASWPU010000380         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_40436,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000380 DASWPU010000000
VERSION     DASWPU010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40436"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VGC27_08210"
     CDS             complement(<1..1095)
                     /locus_tag="VGC27_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0282591.1"
                     /translation="MTKIVEWAVANTRLIGALILVVITAGVYSLNTIPKESDPNVPIP
                     YIWVQVGYPGISPEDSERLILKPLETALRSVEGIKHTQAWAYQGGVYLVMEFMADTPM
                     QRALEDVRAQVDSARARLPKEADPPVVREYSNSDNPVITVALSGNLPERSLLHIVRSL
                     RDDIRMIPAVLEAEISGAREEQLEITIDPAKLETYGITQNEVYAAVSNNNQLIAAGSI
                     ETGHGSFQVKVPGVIATAQDVLDLPIRATADSTITLKNVAQVRRTFVDPYSFARMDGR
                     PTMAIDVTKRVGANIIDTNEKVRAAVAKAQKSWPAGVQATFMFDQSTFIRDQIGSLTD
                     SILLAILLVMIIIVAALGLRSGLLVGVAIPT"
     gene            complement(1102..2226)
                     /locus_tag="VGC27_08215"
     CDS             complement(1102..2226)
                     /locus_tag="VGC27_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0282592.1"
                     /translation="MTWIVGLYERVMPDFWLALKPQYQWAFGIAVFVTLYLATGLLRP
                     GHPAGDAMQAQAKTGHLPIVSVARLNAVPHEATIVLRGRTEALHQVDVRAEVDGVIAA
                     LHFEKGDRVKKGQVLCEIKLNDRGARAAQAEAQMAQSSKELEVARELYKEGFRSKTQL
                     AQATAAFEAAKAGASTMNIQLANTRIRAPFAGIVEERYANVGDYLPVGSKCAMVLAPE
                     PFLAIGTVSEDMVAQIAPGNKARVKLVTGESVEGTVRFVAEHADPATRTFRVEVEMPN
                     PDAKLRSGVSADIMVVPLSKVPAYKISPGVLVLDDTGTVGVRVVQKNTARFLPVQIIS
                     DGPEGMWISGLPATADIIIVGQEFVGNGERVKAVFNKKTW"
     gene            complement(2254..2784)
                     /locus_tag="VGC27_08220"
     CDS             complement(2254..2784)
                     /locus_tag="VGC27_08220"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0282593.1"
                     /translation="MDVRTICLGILTRGDATGYEIKKTVEEDGWRHFVEASYGSIYPA
                     LNRLTKEGLVSVRAEAQEKRPDRKVYTITQAGRHAFFDALMGSLPEDRYRSPFVFAML
                     FADLLPQERVSQMLDAYIARVEETLGHLQQADAKPQRQGEHFANGLGRAGYAAILEYL
                     RAHRAKFEAKTAEAAE"
BASE COUNT          496 a          898 c          941 g          564 t
ORIGIN      
        1 ggtggggatc gccacgccga ccaacaatcc ggagcgcaat cccagcgccg ccacgatgat
       61 gatcatgacc aggagaatcg ccagcagaat ggaatcggtc agcgagccga tctggtcgcg
      121 gatgaaggtg gactggtcga acatgaaggt ggcctggacg cccgccggcc agctcttctg
      181 ggccttggcg acggccgcgc gcaccttttc attcgtgtcg atgatgttgg cgccgacgcg
      241 cttcgtcacg tcgatcgcca tggtcggccg gccgtccatg cgcgcgaagc tgtagggatc
      301 cacgaaggtg cgccgcacct gggccacgtt cttcaacgtg atcgtggaat cggcggtcgc
      361 gcggatcggc aggtcgagca cgtcctgcgc cgtggcgatg acgccgggca ccttgacctg
      421 gaaactgccg tggccggttt cgatcgatcc cgcggcgata agttggttgt tgttggagac
      481 ggcggcatag acctcgttct gggtgatgcc gtaggtctcc agcttcgcgg gatcgatcgt
      541 gatctcaagc tgttcctcgc gcgcgccgga gatttcggct tccagcacgg cgggaatcat
      601 ccggatgtcg tcgcgcaggg agcgcacgat atgcagcagg gagcgctccg gcaggttgcc
      661 ggagagcgcc acggtgatca ccgggttatc ggaattgctg tattcgcgga cgaccggcgg
      721 atcggcctct ttgggcaggc gcgcgcgggc cgaatccacc tgggcgcgca cgtcctccag
      781 cgccctctgc atcggcgtgt cggccatgaa ctccatcacc agatacacgc cgccctgata
      841 ggcccaagcc tgggtgtgct tgatgccctc caccgagcgc aaggccgtct ccagcggctt
      901 caggatcagc cgctcgctgt cttcggggct gatgccggga tagccgacct gaacccagat
      961 gtagggaatg ggaacgttcg gatcggattc cttcggaatc gtgttcagcg aataaacgcc
     1021 ggccgtgatg acgaccagga tcagcgcccc gatcagccgc gtgtttgcca ccgcccattc
     1081 gacgatcttg gtcatgacgc tctaccaggt tttcttgttg aatacggcct tgacccgttc
     1141 gccgttgccg acgaattcct gaccgacgat gatgatgtcc gccgtggccg gaagcccgga
     1201 aatccacatg ccttccggac cgtccgagat gatctgcacc ggaagaaagc gcgcggtgtt
     1261 cttctgcacg acgcgcacgc ccaccgttcc ggtatcgtcc agaacgagga cgcccggaga
     1321 tatcttgtag gcgggcacct tgctcagcgg caccaccatg atgtcggcgc tgacgccgct
     1381 gcgcagtttg gcgtcgggat tgggcatttc gacctcgacg cggaaggtcc gcgtggcggg
     1441 atcggcatgt tcggcgacga agcgcaccgt gccttcgacg ctctcgccgg tgacgagctt
     1501 gacccgcgcc ttgttgcccg gcgcaatctg ggcgaccata tcctcgctca cggtgccgat
     1561 ggcaaggaag ggttccggcg ccagcaccat ggcgcatttc gagccgacgg gaagataatc
     1621 gccgacattg gcgtagcgct cttcgacgat gccggcgaac ggcgcacgga ttctggtgtt
     1681 ggccagctgg atgttcatgg tggaggcccc ggccttggcc gcttcgaagg cggcggtggc
     1741 ctgggcaagc tgcgtcttgg agcggaagcc ttccttgtag agctcgcgcg caacctccag
     1801 ctccttgctc gattgggcca tttgggcctc ggcctgggcg gcgcgcgcgc cgcggtcgtt
     1861 gagcttgatc tcgcacagca cctggccttt cttgacgcgg tcgccctttt cgaaatgcag
     1921 cgcggcgatc acgccgtcca cttccgcgcg gacatcgacc tgatgcagcg cttcggtgcg
     1981 gccgcgcaaa acgatcgtcg cctcgtgcgg cacggcgttg agcctcgcca cgctcacgat
     2041 cggcagatga cccgtcttgg cctgcgcctg catggcatcg cccgccggat gaccggggcg
     2101 caacaggccc gtggcgagat acaaggtcac gaagacggcg atgccgaatg cccattgata
     2161 ttgcggcttc agggcaagcc agaagtccgg catgacgcgc tcatagaggc cgacgatcca
     2221 cgtcattatt cttccgaaca cggtttgctc cacttactcg gccgcctcgg cggtctttgc
     2281 ttcgaatttg gcgcgatggg cgcgcagata ttccaggatg gcggcatagc ccgcccggcc
     2341 caggccgttg gcgaaatgtt cgccttggcg ctgcggcttg gcgtcggcct gctgcaaatg
     2401 gcccagtgtc tcttcaacgc gcgcgatgta ggcgtcgagc atctggctga cgcgctcttg
     2461 gggcagcaga tcggcgaaca gcatcgcgaa gacgaacggc gaacgatagc ggtcctcggg
     2521 cagggacccc atcagggcgt cgaagaaagc gtggcggccg gcctgggtga tggtgtacac
     2581 cttgcggtcc ggccgctttt cctgggcctc ggcgcggacc gagaccaaac cttcttttgt
     2641 cagcctgttc agcgcggggt agatcgagcc gtagctggcc tcgacgaaat gccgccagcc
     2701 gtcttcttcg acggtttttt tgatctcgta tcccgtcgcg tcgccgcggg tcaggatccc
     2761 caagcaaatc gtgcgaacgt ccatgatcgg tctatattgg tttgatatag atcgatcgaa
     2821 tatcggtaca aattgtgggg tttcaatagc aaaaggctac gcggcggaaa cacgtcaggc
     2881 ctgcaccgga cgacgacgc
//
LOCUS       DASWPU010000381         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_8921, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000381 DASWPU010000000
VERSION     DASWPU010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8921"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VGC27_08225"
     CDS             <1..380
                     /locus_tag="VGC27_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0282594.1"
                     /translation="VLGAMPFFARLVETSLREVDHAVIEATRSLGASNRQIVLYTMLP
                     EALPSLIAAVTVTAIALVSYTAMAGVVGAGGLGDLAIRYGYQRFQTDVMVVTVFLIIV
                     LVQAFQMLGDVLVAHFHRRTTPH"
     gene            392..1183
                     /locus_tag="VGC27_08230"
     CDS             392..1183
                     /locus_tag="VGC27_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0282595.1"
                     /translation="MLSRRLFVISGSAVLVGCSSPDKNALSVAATAVPHAEILKFVTP
                     DLAAEGVDLRVKVFNDYVQPNLQVDQKFLDANYFETLPYLNAFNREHGTRLVPVVGVH
                     IEPFGGYSRKVSKIADLRRGAVIALPNDPTNTGRALILLHKHGLITLKDPNSITATQK
                     DVVANPNGFIFKELESPSLPRVLDEVDLALINTNYALDAKLAPHKDALIIEGPDSPYT
                     NYLVARPDNRNDPRIRKLAALLTSAKVADFIRRTYHGAVIPAHKA"
     gene            1191..1658
                     /locus_tag="VGC27_08235"
     CDS             1191..1658
                     /locus_tag="VGC27_08235"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY0282596.1"
                     /translation="MTVESVRAFLAEKAPDIAIIELEDVTATVELAAQGHGVEPAQIA
                     KTLSLRAGGREFLVVMRGDARLDNKKAKATFGGKVSMLGPEEVEAITGHPVGGVCPFG
                     LRMPLPIYCDVSLKAFEEVVPAAGSINSAVRINPLRMAELVGAEWVDVCEMRG"
     gene            1759..>2069
                     /locus_tag="VGC27_08240"
     CDS             1759..>2069
                     /locus_tag="VGC27_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282597.1"
                     /translation="MKRREFIAGLGFAAALPAIARAQQPTPTIGFLSTRSPEEAAIHT
                     DAFRSGLEETGYVEGKSLAIEYRWAQGDYSKLPGFTAELLSKPLALMVAAGDPAALAA
                     KA"
BASE COUNT          382 a          658 c          626 g          403 t
ORIGIN      
        1 tggtgctggg tgcgatgccg ttcttcgcac gcctggtcga aacctcattg cgcgaggtcg
       61 atcacgcggt gatcgaggcc acgcgttcgc tcggcgccag caaccgccag atcgtgctgt
      121 acactatgct gcccgaggcg ctgccaagcc tgatcgcggc cgtgaccgtg accgcgattg
      181 ccctcgtatc ctacaccgcg atggcgggcg tggtcggcgc cggcggcctg ggcgacctcg
      241 ccattcgcta cggctatcag cggtttcaga ccgacgtcat ggttgtgacc gtgtttctca
      301 tcatcgttct ggtgcaggcc ttccagatgc tcggcgacgt cctggtcgcg cacttccatc
      361 gccgcaccac acctcattga aggacaatac catgctctcc cgccgcctgt tcgtgatctc
      421 cggttccgcc gtgctcgttg gctgctcttc gccggacaag aatgcacttt ccgtcgccgc
      481 caccgcggtt ccccatgcgg agattctgaa gttcgtcacg cccgatctcg ccgccgaagg
      541 cgtcgacctg cgggtgaagg tcttcaacga ctatgtgcag ccgaaccttc aagtggacca
      601 gaaattcctg gatgcgaatt acttcgagac cttgccgtat ctgaacgcct tcaatcgcga
      661 gcacggcacc aggctggtgc ccgtcgtggg cgtacatata gagccgttcg gcggctattc
      721 caggaaggtg tcgaagattg ccgatttgag gcggggtgcg gtcatcgcac ttcccaatga
      781 tcccaccaac accgggcgag cgctgatcct gctgcacaag catggcctga tcacgctgaa
      841 ggacccgaac agcatcacgg cgacacagaa ggacgtcgtg gcgaacccga acggcttcat
      901 cttcaaggag ttggaatcgc cgtcgctgcc gcgcgtgctg gacgaagtcg atctggcgct
      961 gatcaacacg aactatgccc tcgacgccaa gcttgctccc cacaaggatg cactgatcat
     1021 cgaaggcccc gacagcccct atacgaacta cctcgtggcg cggcccgaca accggaacga
     1081 tccccgcatc cggaagctcg cggccttgtt gacctccgcc aaagtcgccg atttcattcg
     1141 ccggacctat cacggtgctg tgattccggc gcacaaggca tgagaagctc atgaccgtcg
     1201 aatccgtccg cgcgttcctg gccgagaagg cgcccgatat agccatcatt gagttggagg
     1261 acgtcaccgc caccgtggaa ctcgcggcgc aaggccacgg cgtcgaaccg gcgcagatcg
     1321 caaaaaccct gtccctacgc gccggaggcc gagagtttct cgtcgtcatg cgcggcgacg
     1381 cgcggctcga caacaagaag gccaaggcga cgttcggcgg caaggtcagc atgctcgggc
     1441 cggaggaggt ggaagccatt accggtcatc cggtgggtgg tgtttgtccg ttcggtctgc
     1501 gtatgccgct tccaatatac tgcgacgtat cgctcaaggc gttcgaggaa gtcgtgccgg
     1561 ccgcgggttc gatcaacagc gcagtgcgca tcaatcccct gcgcatggcc gaactcgtcg
     1621 gcgccgaatg ggtcgatgtt tgcgagatga gaggctgagc cgtgcaagcg aaaattttga
     1681 tcaccgctca tgcgtatttc gcctccgtcg ggattttcgt ttgctggtag gatgcctcct
     1741 gtgattttcg gggaagccat gaagcggcgc gaattcatcg cggggttggg atttgcggcg
     1801 gccctgcccg caattgcgcg cgcccaacaa cccacgccga ccatcggatt tctctcgacc
     1861 cgttcgccgg aggaagccgc catccatacc gacgcctttc gcagcggcct cgaagagacg
     1921 ggttatgtcg agggcaagag cctcgccatc gaatatcgct gggcgcaggg cgactacagc
     1981 aagctgcctg gatttacggc tgagctactg agcaagccgc ttgcgctgat ggtggccgcg
     2041 ggcgatccgg ctgcactcgc ggccaaggc
//
LOCUS       DASWPU010000382         5165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_58115,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000382 DASWPU010000000
VERSION     DASWPU010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58115"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VGC27_08245"
     CDS             <1..985
                     /locus_tag="VGC27_08245"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEY0282598.1"
                     /translation="GRYRLGATINFNEPLGIGDVLSARILGSTTGGLAYGRLSYQAQV
                     EDGTVGVAYTAFQYRLGRQFSSLNARGSEQIASIYGSYPLIRSYDSNLYALLNFDYRY
                     FHDTIGATSTVTNKQAWVAMPGISGDHHDTFGGGGWTSYALIGSIGNLDIQSPLARAI
                     DAATARTNGTYEKLYFSVSRLQNVIGPLSLYGSVRGQIASKNLDVSEKMELGGAYGVR
                     AYPEGESYGDEGYMATLEARLLLPRWIEELPGELQAIAFVDTGTVTLNKSPWYTGSNS
                     ETRSGAGVGLIWAATNNFIASATYAWQLGNQPATSAPDSFGGFWVQIVKYF"
     gene            1185..3398
                     /locus_tag="VGC27_08250"
     CDS             1185..3398
                     /locus_tag="VGC27_08250"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEY0282599.1"
                     /translation="MAILGAAGLLSLGLGSTAYALPTSGAVSAGAASISSTSSTLTVN
                     QSTQNAAINWQSFGIAQGEAVTFQQPNSNSVTLNRVVGADPSAILGSLSANGKIFLVN
                     PNGVLFAQGAQVNVGGLVASTLGITDANFMTANYKFSGSSSSPILNQGAIHADGGYVA
                     LLGANVNNQGVIVAKLGSVTLAAGSAMTLDVAGNGLLNVAIDQGAVNALAQNGGLIQA
                     NGGQVLMSAKSAGELLKSAVNNTGVIEAQTIENHNGSIKLMGDMQSGTANISGTLDAS
                     APSGGNGGFIETSAATVNIADDVRITTAAVNGLTGTWLIDPVDFTIGSGGNISGATLS
                     AQLVTNSVNISTSTGSDATVPGTPPTTSYYTATTGNGDINVNDAVSWTAAPSTTTLTL
                     TADRDVNINNDITATNGNLVVCCGRDINENASITTTNGSVLLSAGRNVNLTAVGAMTT
                     TDGNITICAGTDINVNKAITLTRGSVTPAQSLGLATGLVMIAGNAGTGPGVASGTVIF
                     DPLSPQVSVTGPNAPVLINYNPVSYATPTNYSSNFTLTGGATLTQQMLVFPDGDKVED
                     GTTAATLTGFKSTADTGIPAGVTLVAGSGSTAVFDSADVGTDIGITYSGYSLAGADAA
                     NYALAANCCTSGFRTSGTITAAVVTPPVVTPPVVTPPVVTPPVVTPPVVTPPDVTPIE
                     FLPVQMFVPLGYSPVVEAPRFTVLAGGVRMPAPPAPVVVPPVYVPPVYPPKQDRY"
     gene            3435..4187
                     /locus_tag="VGC27_08255"
     CDS             3435..4187
                     /locus_tag="VGC27_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282600.1"
                     /translation="MSMTAAGRIGLVLAAAILAWPPLAAAGDASRLAVASPASSDASV
                     HFAPLDPLSPTDRPPRADFLGEGASNDSRRVADWIAASGDNGGLPYLIIDKIQAKVFA
                     FDNDGRLLGATVALLGKARGDDTVPGIGSRKLSAIRPHERTTPAGRFVADLGRDFGQD
                     ILWIDYDASVSLHRVIKGDPGDRRLQRLATASPLDKRISYGCINVPVNFYEQIVRKTF
                     TGTSGIVYILPETKAIQEVFSMSEGALGNGTR"
     gene            4494..>5165
                     /locus_tag="VGC27_08260"
     CDS             4494..>5165
                     /locus_tag="VGC27_08260"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEY0282601.1"
                     /translation="MNRTTSPGRISALILLVSVVLTASAAAEAVPPAKPDSRFLQPLP
                     LNFDDHEGFASIFDGTLDGWDGDRRFWRIEDGNIVGESTPDNPAGNSYLTYRKLTAHD
                     FDLRLEIKVEKTGGSGIQYRSRTGVPWRGKTPPAVLANVGPYNSDTMLTGPQADFQYP
                     VSPKVFAYNGQVFAENSPMGVEAWLGQVVRQYGADQSKKKLVGTIAPQRSLIGAVNGN
                     DWNQYE"
BASE COUNT         1074 a         1670 c         1502 g          919 t
ORIGIN      
        1 ggggcgttac cgcctcgggg ccacgatcaa cttcaacgaa cctctcggaa tcggagacgt
       61 actcagcgcc cgcatcctcg ggtccacgac gggcggcttg gcctatggcc gcctgtccta
      121 ccaagcccaa gtcgaggacg ggacggtggg tgttgcctac acggcgttcc agtaccggct
      181 gggccggcag ttttccagct tgaatgcccg cggctcggag cagatcgcca gcatctacgg
      241 cagctatccg ttgatccgct catacgacag caatctctat gctctgctca atttcgacta
      301 cagatacttc cacgacacga taggcgccac ctccaccgtc acgaacaaac aagcgtgggt
      361 agcgatgcct gggatcagcg gcgaccatca cgatacgttc ggcggcggcg gatggacctc
      421 atacgcactg atcgggagta tcggcaatct cgacattcaa agtcccctgg cgcgggcgat
      481 agacgcggcg accgcgcgga ccaacggaac gtacgaaaag ctctatttca gcgtttcccg
      541 gctacagaat gtgatcggtc ccctctctct ctacggctcg gtcagagggc agatcgcctc
      601 caagaacctg gacgtgtccg agaagatgga gctgggcggc gcctatggcg tgcgcgccta
      661 cccggaaggc gaatcctatg gcgacgaggg atatatggcg accctggaag ccaggctgct
      721 gctgccaaga tggatagagg aattgcccgg cgagctgcag gccattgcct ttgtcgatac
      781 cggcacggtc actttgaaca agtctccctg gtacacgggg tccaacagcg aaacccgcag
      841 cggagccggc gtcgggctta tttgggccgc caccaacaat ttcatagcgt ccgcaaccta
      901 cgcctggcaa ctcggcaatc agcccgcaac gtccgcgccc gacagcttcg gcggcttctg
      961 ggtacaaatc gttaagtatt tctagtgttg atttgttaat taatagggtc gcatctcctt
     1021 gagccggggt atcgctttgg actcaaggaa ctgccgctat gagatatacc ccgagcccta
     1081 ctgggcatga gagcacgaat tgctctggcg ccgccgccaa caacgtcacg gtcaagctga
     1141 ggcaagcctg gttacgctcg acggctttgg ccggatttgc gcgtgtggca attttggggg
     1201 cggccggact cttgtcgctc ggcctcggat cgacggccta tgccctgccg acaagcggcg
     1261 ccgtctcggc gggcgccgcc agtatttcga gcaccagctc cacgctcacg gtcaatcagt
     1321 cgactcagaa cgcggcgatc aactggcaga gcttcggcat cgctcaaggc gaggccgtaa
     1381 ccttccagca gcccaacagc aattcggtga cgctcaaccg cgtcgtgggc gccgatccgt
     1441 cggccatcct gggcagcctg tccgccaacg gcaagatttt cctggtgaac cccaacggcg
     1501 ttctgtttgc ccaaggcgcg caagtaaacg tagggggcct tgtagcctcc acgctcggca
     1561 tcaccgacgc caacttcatg acggccaatt acaagttctc cggctccagc agcagcccaa
     1621 tcctcaatca aggcgccatt catgccgacg gcggctacgt cgcgctgctg ggcgccaacg
     1681 tgaacaacca aggcgtgatc gtcgccaaac tgggctcggt gacgcttgcc gcgggaagcg
     1741 ccatgacgct cgacgtcgca ggcaacggct tgctcaatgt ggctatcgat caaggcgcgg
     1801 tgaacgcgct cgcccagaac ggcggcttga tccaggccaa tggcggacag gtgctgatga
     1861 gcgcgaagtc cgccggcgaa ctgctcaaga gcgccgtgaa caacacgggt gtcatcgagg
     1921 cacagaccat cgaaaaccat aacggctcta tcaagctgat gggagacatg caaagcggca
     1981 ccgcgaacat cagcggtacg ctggacgcca gcgctcccag cggcggcaac ggcggcttta
     2041 tagaaacctc cgccgcgacg gtgaatatcg ccgacgatgt aagaatcacc accgccgccg
     2101 tcaacgggtt gacgggcacc tggctgatcg acccggtgga tttcacaatc ggctccggag
     2161 gcaacatctc cggcgcgaca ctctccgccc aattggtaac gaatagcgtc aatatcagca
     2221 cctcaaccgg atccgacgcc acggtccctg gaacccctcc cacgaccagt tattacacgg
     2281 cgacgaccgg caacggcgac atcaacgtaa acgatgcggt cagctggacg gcggccccca
     2341 gcacaaccac gttgacgctg accgccgatc gcgatgtgaa catcaacaat gacatcaccg
     2401 ccacgaacgg aaatctcgtg gtgtgttgcg gacgcgacat caacgagaat gcgtccatca
     2461 ccacgacaaa cggcagcgtc ctgttgagcg ccggacgcaa tgtaaatctg actgcggtcg
     2521 gagccatgac gacgaccgac ggaaacatca cgatatgcgc cggaacggac atcaacgtga
     2581 acaaggctat tacgctgacc agaggcagcg tcaccccggc acagagcctc ggcctggcga
     2641 ccggtctggt catgatcgcc ggcaacgccg gcaccggccc gggtgtcgcc agcggcaccg
     2701 tgatcttcga tcctctctcc ccacaggtga gcgtcacggg accgaatgcg ccggtgctca
     2761 tcaattacaa tccggtctcg tacgcaacac ctacaaatta ttcgtcgaac ttcaccttga
     2821 ccggaggcgc cacgctcacc caacagatgc tggtcttccc ggacggcgac aaggttgaag
     2881 acggcacgac cgcggcaacc ctcaccggct tcaagagcac cgccgacacg ggcattccgg
     2941 ccggggtcac attggtagcc ggctccggaa gcaccgccgt tttcgatagc gcggacgtag
     3001 gaacagacat cggcatcact tacagcggct atagtcttgc cggcgccgac gcggccaatt
     3061 acgcgctcgc agccaattgc tgcacttcgg gttttagaac aagcggtact attacagcgg
     3121 cggtcgtgac gccgccggtc gtaacgccgc cggtcgtaac gccgcccgtc gtgacaccgc
     3181 cggtcgtaac gccacccgtc gtgacaccgc ccgacgtgac gccgatagag tttctcccag
     3241 ttcaaatgtt cgtcccgtta gggtactcgc cggtggtcga agctcctagg ttcaccgtgc
     3301 tcgccggtgg cgtcagaatg ccggccccgc cggctcctgt tgtcgtgccc ccggtctatg
     3361 tgccgcctgt gtatccgccg aagcaagatc ggtactagag cccacgtctg gccgtgcgcg
     3421 aaatggcgaa aacaatgtct atgactgccg ccgggagaat tggactggtc ctggcggcgg
     3481 ccatactggc atggccgccc cttgctgcgg cgggcgacgc ttcgcgcctc gccgtagcca
     3541 gtcccgccag ctcagacgcg tccgttcatt tcgcaccgct tgacccgttg tcgccgacgg
     3601 atcggccgcc aagggcggat tttcttggag agggcgcctc gaacgactcg cggcgcgtcg
     3661 cggattggat cgcggcttcc ggcgacaatg gcggcttgcc ctacctcatc atcgacaaga
     3721 tccaggcaaa agttttcgcc ttcgataatg acgggcggct gctcggagcc accgtcgcgc
     3781 tcttgggcaa ggcccgtggc gacgacaccg taccgggcat cggcagccga aaattatcgg
     3841 ctatacgccc gcacgaacga accaccccgg cgggccggtt cgtggccgat ctgggccgcg
     3901 acttcgggca ggatattctc tggatagatt acgacgcctc cgtctctctg catcgtgtga
     3961 tcaaaggcga ccccggagat cgccgattgc agcgattggc gacagcatcc ccgctcgaca
     4021 agcgaatttc ctatggctgc atcaacgtgc ctgtaaattt ctacgaacaa atcgtgcgca
     4081 aaacattcac cggcacgagc ggtatcgtct acatcttgcc ggaaaccaaa gcgatccaag
     4141 aggtcttttc catgtccgaa ggcgccctcg gcaacgggac gcggtaagct cggcgaggat
     4201 tcgcatcgac ctgatctgct ggtcttcacc catgaccgga tcgttgatgg accaatctaa
     4261 agctgtggtt cgcgatcatc catcgtgaag ggtgaaacat acaaccgtga cgaacgggaa
     4321 acacgacggc cgtgcaaccg cacggccgtc gtgtatccac agtgcattgc cggtcgcccc
     4381 ccgcgaattg ccgcgccggc ttccatcgca ccttctcttc atccatgttg tacaaagctc
     4441 gcagccggga cgtacttggc cgcgccaacg tcggccgaaa tggggagcac atcatgaaca
     4501 gaacgacttc gccgggcagg atttcggcac tgatactctt ggtaagcgtg gtcttaaccg
     4561 cgtccgcggc agcggaggcc gttccgccgg cgaaacccga ttcccggttc ctccagccgc
     4621 tgccgctgaa cttcgacgac catgagggtt tcgcttcgat cttcgacgga acgctcgacg
     4681 gctgggacgg cgaccgccgg ttctggcgca tcgaagacgg aaacatcgtc ggcgaatcca
     4741 ctcctgacaa tcccgccggc aattcctacc tgacctaccg caagctgacc gcgcacgatt
     4801 tcgatctgcg cctggagatc aaggtcgaga aaaccggggg cagcggcatc cagtatcgca
     4861 gccgcaccgg ggttccttgg cgcggcaaga ctccgcccgc cgtcctcgcc aatgtggggc
     4921 cttacaacag cgacacgatg ctgaccgggc cgcaagcgga cttccagtat ccggtcagcc
     4981 ccaaggtttt cgcctataac ggccaagtct tcgcggaaaa cagccccatg ggcgtcgaag
     5041 cctggctcgg ccaggtggtg cgccagtacg gcgccgatca atcgaagaag aagctggtcg
     5101 gcaccatcgc gccgcaaagg tcgctgatcg gcgccgtcaa cggcaacgat tggaaccaat
     5161 atgaa
//
LOCUS       DASWPU010000383         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_95135,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000383 DASWPU010000000
VERSION     DASWPU010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95135"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..>1699
                     /locus_tag="VGC27_08265"
     CDS             30..>1699
                     /locus_tag="VGC27_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0282602.1"
                     /translation="MQNIESRMGIPQCGDESSGREAGAAIHTGDVPTMKSFLLAIFGA
                     SLLVCLPSGVLAQLDQGQIGGVVTDGSHAVVQNATVQVISAAKGTIQSTHTDSNGLYS
                     VTNLPVDVYNVVIESAGFRLYKQTGITVDAGARVTANAVLEPGAAEQTIIVRSTGAEV
                     ETDTAMVGRTIDTSQMKDLALNGRNPINMALTKAGVTGGSFNQFNPDDLGANGFTING
                     SSSTENAVTLDGVNAVRTRSGTATIGVFNVDAIQEVQILAANYPAEYGRVDGGQIRFV
                     SKSGTSDFHGTVFEFFRNSALDANSWVRNDSPDPTQNSKPDGLRFNQPGFSLGGPVYW
                     PGRWNERKDKLFFFAAEEWVRFRQDQTNTGTVPTALMRQGNFSELLDPNNPFTNSVQV
                     VMNPATGQPFGNNTIGDPALLSKNGLALLSAFPLPTPGFQDGAANWIESLPAPRNSRK
                     DFYRVDYYAGRNRYTLTGQHYAYESISPFTSDLDRFGTDQLRPNDTGAFNITSTFSPK
                     WLNDLTVSASNDLVNTKTLQSRPYERNLYGIDYPYIFPGIKDVEDKIPT"
BASE COUNT          381 a          542 c          482 g          294 t
ORIGIN      
        1 ggctcatgcg aaagtcaggg aggaacaaca tgcagaacat tgaatcgagg atgggaatac
       61 cccaatgtgg cgatgagagc tcgggccgtg aagccggggc cgcaatccac acaggagacg
      121 ttcccaccat gaagtcattc ctattggcca tctttggcgc atcgctactc gtttgcctgc
      181 cgtcgggcgt gctcgcccag ctcgaccagg gacagatcgg tggcgttgtt acggacggtt
      241 cccatgccgt tgtgcagaac gccacggtgc aggttatcag cgcggcgaag ggtacgatcc
      301 agagcaccca cactgactcc aacggtctat atagtgtcac caatcttccg gtcgacgttt
      361 acaacgtcgt aatcgaaagc gccggattca ggctctataa acaaaccggc atcacggtcg
      421 atgcgggcgc ccgtgtcacc gccaatgccg tcctcgagcc gggcgccgca gagcagacga
      481 tcatcgtgcg gtccacaggc gcggaggtag agaccgatac ggctatggtc gggcgcacca
      541 tcgacacctc acagatgaag gacttggccc tcaatggccg caacccgatc aacatggcgc
      601 tgaccaaggc cggcgtgacc ggcggcagct tcaaccagtt caacccggat gacctgggcg
      661 cgaacggctt caccatcaac ggcagttcca gcacggaaaa tgctgtcaca ctcgacggcg
      721 tcaacgcggt acgtacccgc tcgggcacgg cgacgatcgg cgtcttcaat gtagacgcga
      781 tccaggaggt acagatcctg gccgccaact acccagccga gtacggacgc gtggacggcg
      841 ggcagatccg cttcgtctca aaaagcggca ccagcgactt ccacggcacg gtcttcgagt
      901 tcttccgcaa ctcggcgctc gatgccaaca gttgggtgcg caatgacagc ccggacccga
      961 cgcagaacag caagcccgac ggactacgct tcaaccagcc cggcttctcg ctgggcggtc
     1021 cggtctactg gcccggcaga tggaatgagc ggaaggacaa gctgttcttc tttgccgctg
     1081 aggaatgggt gcgttttcgc caggatcaga ccaacaccgg cacagtgcca acagcgctga
     1141 tgcgccaggg taacttcagc gaactgctgg acccgaacaa tcccttcacc aactcggtac
     1201 aggtggtgat gaatcccgcg accgggcagc ccttcggcaa caatactatc ggcgacccgg
     1261 ccctgctgag caagaatggc cttgcactgt tgagcgcatt tccgttgccc acgccgggct
     1321 tccaggatgg cgcggccaac tggatcgagt cgctgccggc gccacgtaac agccggaagg
     1381 acttctaccg tgtcgattac tacgcgggcc gcaaccgtta cacgctgacg gggcagcact
     1441 atgcgtatga gtcgatcagc ccgttcacat cggacctgga tcgctttggc accgaccaac
     1501 tgcgcccgaa cgacaccggg gccttcaata tcaccagcac cttctcaccc aagtggctca
     1561 acgacctgac cgtgtcggca tcgaacgatc tggtcaatac caaaacactc cagagccgtc
     1621 cctacgagcg caacctctac ggcatcgatt atccctacat cttcccgggc atcaaagatg
     1681 tggaagacaa gattccgac
//
LOCUS       DASWPU010000384         3730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_88663,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000384 DASWPU010000000
VERSION     DASWPU010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88663"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..694
                     /locus_tag="VGC27_08270"
     CDS             134..694
                     /locus_tag="VGC27_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017532211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY0282603.1"
                     /translation="MFIQTESTPNPATLKFLPGREVMGEGAVADFPSRQAAGRSPLAR
                     ALFETPEVERVFFGSDFISVTKAGGDWKHLKPAILGAIMEHFTRELPLIEAGAEEADA
                     AASGDGDSEIVLQIKDLIETRVRPAVAQDGGDIVFKGFDDKSGIVFLHMRGSCAGCPS
                     STLTLKNGIENMLRHYIPEVSAVEAV"
     gene            752..1342
                     /locus_tag="VGC27_08275"
     CDS             752..1342
                     /locus_tag="VGC27_08275"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:NF003768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEY0282604.1"
                     /translation="MAHAINDEALDTIFRAARTHNIWLDKPVSPTLLMAVYDLMRWGP
                     TSANTSPARIVFVVSKEAKERLKPHLGAANVDKTMAAPATAIIGYDLDFARHMPKLFP
                     HNPGAKDWFKDPAFAKTTAFRNGALQGGYFIIAARALGLDCGPMSGFDNEGVDREFFA
                     GTAIKSNFLCNVGYGDEAGLFARGPRLSFDEACKIV"
     gene            1355..2011
                     /gene="tsaB"
                     /locus_tag="VGC27_08280"
     CDS             1355..2011
                     /gene="tsaB"
                     /locus_tag="VGC27_08280"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY0282605.1"
                     /translation="MKILAVDTALGACSAALLDGNRILARRFAAMERGHAEALAPMVE
                     AVMDEAGLAFPALDRLAVTVGPGTFTGQRVGLAFMRGLRVALGKPLVGVTSLAAMAEQ
                     ARAETGVACAAAIHDARRAEVYLEILNPPLSARLLSFIEACAVLSGLRGKLVLAGTAA
                     ARAAEALQTSGLETQVCGIVAPDAVWVARLAAGMEPGESPPRPLYLRAPDAKLPARKG
                     "
     gene            2008..2484
                     /gene="rimI"
                     /locus_tag="VGC27_08285"
     CDS             2008..2484
                     /gene="rimI"
                     /locus_tag="VGC27_08285"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY0282606.1"
                     /translation="MKTVLLTPPCDVDILARLHAASFADAWSSEWIAKLLVLPGTFAL
                     LAGGESGGFVLARAAGGEAEILTLAVAPAARRRGIGSALILTACRHAREMGAGTMYLE
                     VSRTNDAAKALYTQLGFREVGKRRGYYQEPGGSKEDALVFCVKLPLPQVGNRERFD"
     gene            2512..2922
                     /locus_tag="VGC27_08290"
     CDS             2512..2922
                     /locus_tag="VGC27_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020056161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY0282607.1"
                     /translation="MTRIEKLCVDKGLRMTEQRRVIARVLSDAADHPDAEELYRRASA
                     LDPHISIATVYRTVKLFEDAGILERHDFRDGRSRYEEIPESHHDHLIDVHTGRVIEFH
                     NDEIERLQRRVAEELGFELIDHRLELYGVPKKSR"
     gene            2931..>3730
                     /locus_tag="VGC27_08295"
     CDS             2931..>3730
                     /locus_tag="VGC27_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0282608.1"
                     /translation="MPTLRALFLLTVFTLVTLFGVLWQKFLLRFKFKQCKTFPNHFHH
                     FLCRLFGIRITVIGEPVRDRGVLMVGNHASYLDILILSAIARVSFVGRSDLENWPVLG
                     PLAKLQETVFIVRTRRSGAGAARDAIRERLLQGDALVLFPEGTSTDGNRVIRFKSSLM
                     GAAETEMGSDAAGHPYAVPVQPFSLAYVGMHGMPLGRENRPFYAWYGDMELLPHLWEG
                     LKAGPLDVVAEFYPPIAVDGVGGRKKVAAMAEEIVRRGLQRALAGGPPA"
BASE COUNT          661 a         1126 c         1207 g          736 t
ORIGIN      
        1 gccggtcccg agccaacccg ttacggcgat tgggaaaaga agggaattat ttcggacttt
       61 tagccgcctt gatccgccgg cctgccgggt ccatgtaaga gatagattgg aacggctcca
      121 gaaagcggaa aacatgttca tccagacaga atccacaccg aatccggcaa ccctgaaatt
      181 cctgcccgga cgcgaggtca tgggcgaagg cgcagtggcg gattttcctt cgcgccaggc
      241 cgccgggcgc tcgcccttgg cgcgggccct gttcgaaaca cccgaggtgg agcgcgtctt
      301 tttcggctcc gatttcattt cggtgaccaa agccggcggc gactggaaac acctcaagcc
      361 cgcgattctc ggcgcgatca tggagcattt cacccgcgag ctgccgctga tcgaggcagg
      421 cgccgaggaa gccgatgcgg cggcttccgg cgacggcgat tcggagattg tcttgcagat
      481 caaggacctg atcgagacgc gcgtacggcc tgcggtggca caggatggcg gcgacatcgt
      541 cttcaaaggc ttcgacgaca agagcggcat cgtgttcctg cacatgcgcg ggtcgtgcgc
      601 aggctgcccg tcttcgaccc tgacgctgaa gaacgggatc gagaacatgc tgcggcatta
      661 cattcccgag gtgagcgcgg tggaagcggt ttaatctcgc cgccgtttct gcgattttcg
      721 cagggcatcg attcgcttga aggagcgcac cgtggcccac gccatcaacg acgaagccct
      781 cgacacgatc tttcgggccg cgcgcacgca caacatttgg ctggacaagc cggtcagccc
      841 gaccttgttg atggcggtct acgatctgat gcgctggggg ccgacgagcg ccaataccag
      901 tccggcgcgg atcgtattcg tggtttcgaa ggaagccaaa gagcgtctga aaccccatct
      961 cggcgcggcc aatgtcgaca agacgatggc ggcaccggca acggcgatca tcggctacga
     1021 tctcgatttt gcccgccata tgccgaaatt gtttccgcac aatcccggcg ccaaggattg
     1081 gttcaaggat cccgctttcg ccaagacaac agcctttcgc aatggtgccc tgcaaggcgg
     1141 ctatttcatc atcgccgcgc gggcgctggg gctggattgc gggccgatgt cgggtttcga
     1201 caatgaaggc gtcgaccgcg aattcttcgc cgggacggcg atcaaatcga actttctgtg
     1261 caatgtcggc tatggcgatg aggccggcct gttcgcgcgc ggccccaggc tgtcgttcga
     1321 cgaggcatgc aagattgtct gagagcgggc ggacgtgaag attcttgccg tcgacacggc
     1381 gctgggcgct tgctccgccg cactcctgga cggaaatcgc atccttgcgc gccgtttcgc
     1441 ggcgatggag cgcggccacg ccgaggcgct ggcgccgatg gtcgaagcgg tgatggacga
     1501 agcgggcctc gccttcccgg cgctcgaccg gcttgccgtc acggtcggtc cggggacatt
     1561 caccggtcag cgcgtcgggc ttgccttcat gcgcgggctg cgcgtggcgc tgggcaagcc
     1621 gcttgtcggc gtaaccagct tggcggccat ggccgaacag gcgcgcgccg agacgggcgt
     1681 ggcgtgcgcg gcggcgatac acgacgcccg ccgcgccgag gtttatctcg aaatcctgaa
     1741 tcctcccctt tccgcgcgtc ttctgagctt catcgaggct tgcgccgtac tgagcggctt
     1801 gcggggaaag ctggttcttg caggcacggc ggccgcgcgc gcggccgagg cgttgcaaac
     1861 atccggtctt gaaacgcaag tttgcggcat cgttgctcct gatgccgtct gggtcgcccg
     1921 cctggcggcg ggcatggaac ccggcgaatc tcctccccgc cctctttatc tgcgagcgcc
     1981 cgacgcgaag ttgccggcga ggaaaggatg aaaaccgttc tgcttacccc tccatgcgat
     2041 gtggatattc ttgcccgtct gcacgcggcg tcttttgccg acgcctggag cagcgaatgg
     2101 attgccaaac tgctggtgct tcccggcact tttgccttgc tggcaggggg ggagtccggc
     2161 ggcttcgtcc tggcccgcgc cgccggcggc gaggcggaaa tacttaccct ggcggtggcg
     2221 cctgcggcgc gacgacgcgg catcggctcg gctttgattt tgaccgcctg ccggcatgcc
     2281 cgggaaatgg gtgccggtac catgtacctg gaagtcagcc gaacgaatga cgccgcgaaa
     2341 gctctgtata cgcaacttgg ctttcgcgaa gtgggcaagc gcaggggcta ttaccaggag
     2401 cccggcggga gtaaggaaga tgcccttgtc ttttgtgtaa aacttccgct accccaggtg
     2461 ggaaaccgtg agcgattcga ctagaatccc ttttatgaag aggagcggac cgtgacgcgg
     2521 atcgaaaaac tctgcgtgga caagggattg cggatgaccg aacagcgccg cgtgatcgcg
     2581 cgcgttctgt cggatgcggc cgatcatccc gatgccgagg aattatatcg ccgcgcttcg
     2641 gcgctcgacc cgcatatttc catcgccacc gtctatcgga cggtgaagct gttcgaggat
     2701 gccggcattt tggagcgcca cgatttccgc gacggtcgtt cgcgctatga ggagattccg
     2761 gaatcccatc acgaccatct catcgatgtg cacaccgggc gcgtcatcga attccacaat
     2821 gacgagatcg agcgcctgca acgccgcgtg gccgaggaac tcggtttcga actgatcgat
     2881 caccgcctcg agctttacgg cgtgcccaag aagtcgcgct gacgccgctt atgcccacgc
     2941 tgcgcgcgct cttccttctt acggtcttca cgcttgtgac gctgttcggc gtgctctggc
     3001 agaagtttct gctgcggttc aaattcaaac agtgcaagac gtttcccaac cattttcatc
     3061 actttctgtg cagactgttc ggcatccgca tcaccgtgat cggcgagccg gtccgggacc
     3121 gcggcgttct gatggtcggc aatcatgcgt cctatctgga cattctgatc ctttcggcca
     3181 ttgcgcgcgt ctccttcgtc ggcagaagcg atcttgagaa ctggccggtg ctcgggccgt
     3241 tggcaaagtt gcaggagacg gtcttcatcg tgcgcacaag acgcagcggc gcgggtgcag
     3301 cgcgcgacgc catccgcgaa cgtctgctcc agggcgacgc actggtgctt tttcctgaag
     3361 gcacatcgac cgacggcaac cgtgtgatca ggttcaaaag ttcgctgatg ggcgcggcgg
     3421 aaacggagat gggaagcgat gcggccggcc atccatacgc cgtgccggta cagccgtttt
     3481 cgcttgccta tgtgggcatg cacggcatgc ccttggggcg cgaaaatcgt ccgttctacg
     3541 cgtggtacgg cgacatggaa ttgctgccgc atctatggga ggggttgaaa gcgggtcctc
     3601 tcgacgtggt ggcggagttc tatccgccca ttgctgtcga tggcgtgggc gggcgcaaga
     3661 aagtcgcggc catggccgag gagatcgtgc gcagagggct gcagcgggcc ttggcgggcg
     3721 gcccgcccgc
//
LOCUS       DASWPU010000385         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_88696,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000385 DASWPU010000000
VERSION     DASWPU010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88696"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VGC27_08300"
     CDS             <1..756
                     /locus_tag="VGC27_08300"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0282609.1"
                     /translation="ARQDGMKGSFLGPSYSQSEIEKRLAAAGAKFDVFADDELLEKTV
                     DALDGGQAVGWFQGRMEFGPRALGNRSILGDPRKPDMQRQLNLKIKYRESFRPFAPSV
                     PVEDLADYFELDCESPYMTFVAEVRKERRRAMTSAEQTLFGIEKLNIPRSDIPAVTHV
                     DYSARIQTVHKETNPRYHALLEAFKRRTGCSVLVNTSFNIRGEPIVCTPEDAFRCFMG
                     TSMELLVAGDCILRKDEQKAVANEDYLKAFELD"
     gene            complement(734..1864)
                     /locus_tag="VGC27_08305"
     CDS             complement(734..1864)
                     /locus_tag="VGC27_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282610.1"
                     /translation="MSKKILFVTHALESRYGAATSLRLLLEHYSGVEADLLVPRSFRR
                     PRDAAATAAPFPSVRRVYELSLPVDLGLLGIGRSFADKLHGTVHWLSWQRDRNRFRDL
                     LERNAYDIVHFNSPVLHGMLEPGMPAVTHMRDIVIDPNSPAVDKIANGLGIVFIDAAT
                     KKPFERVARVMHSATLNNPIDMTGVGLFAGAFRHPRLTERTTIFSMIGRVSAIKGGEL
                     VIKGFRQGAGEDALLLVVGDGPKDYVAHCRMLAGGDPRIVFWGEEENVKKIYAASDYI
                     VRGDPKPCIGRTVYEGLYSGCRVIMPGPGEPGLLFEAERFRASVEFYRAGDAASLAAV
                     FSSCTGKKVPARRYFSNVEDYVRAFDAFLDECLARLNPAQKP"
     gene            complement(1908..>2491)
                     /locus_tag="VGC27_08310"
     CDS             complement(1908..>2491)
                     /locus_tag="VGC27_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0282611.1"
                     /translation="LGDCGTFSFYPSKNLGAYGDAGFVTCAADDTAAVLRKLRDHGRT
                     SKYEHQIVGYNQRMDALQGAILSVKLRHLDAWIAARRRVAARYDGRLRPAGFKTIEPA
                     DGSAPVYHLYVFETSNREEVAKALAQEGIETGIHYPLPLHLQPAFADLSKPASLPVTE
                     RVAGRVLSLPMYPELDNEDVDRICEIVLRHARC"
BASE COUNT          504 a          758 c          779 g          450 t
ORIGIN      
        1 gcccgccagg acgggatgaa agggagtttt ctcggtcctt cctattccca aagcgaaatc
       61 gagaaaaggc tggccgcggc gggtgcgaaa ttcgacgtct tcgcggacga cgaactgctg
      121 gaaaaaacag tcgatgcgct ggatggcggg caggcggtgg ggtggtttca aggccggatg
      181 gagttcggcc cgcgcgcgct cggcaaccgt tccatcctcg gcgatccgcg caagccggac
      241 atgcagcggc agttgaatct caagatcaaa taccgggaga gcttccgccc cttcgcgccg
      301 tcggtcccgg tggaggatct tgccgactat ttcgaattgg attgcgaaag cccctacatg
      361 acgttcgtgg ccgaggtgcg aaaggaacgc cgccgcgcca tgacgagcgc cgagcaaacg
      421 ctcttcggca tcgaaaaact caacatcccg cgctccgata ttcccgcggt cacgcacgtc
      481 gattattccg cgcgcattca aaccgtgcat aaggaaacca atccccgcta tcacgcgctg
      541 ctcgaggcct tcaagcgccg caccggctgc tccgtgctgg tgaacaccag cttcaacatc
      601 cggggagagc cgatcgtctg tacgccggaa gacgcctttc ggtgctttat gggaacgagc
      661 atggagctgc tcgtggccgg cgactgcata ctccggaaag acgagcaaaa agccgttgcc
      721 aacgaggatt atctcaaggc ttttgagctg gattgagacg cgcgaggcac tcgtccagga
      781 aggcgtcgaa ggcgcgcaca tagtcctcga cattactaaa atatcggcgg gccggaactt
      841 tcttcccggt gcaagacgaa aagacggccg cgagcgaggc ggcatcgccc gcccgataga
      901 attcgacgga agcgcgaaac cgttccgctt cgaaaaggag gccgggctct cctggtccgg
      961 gcatgatcac cctgcagccg gaataaaggc cctcgtagac cgtgcgcccg atacagggct
     1021 tgggatcgcc gcggacgata taatcgctgg cggcgtaaat cttcttcaca ttctcctcct
     1081 cgccccagaa cacgatcctg ggatcgccgc cggcaagcat tcggcaatgc gcgacataat
     1141 ccttggggcc gtccccgacg acgagcagca gcgcatcctc gccagcgccc tgcctgaacc
     1201 cctttatgac aagttcgccc ccctttatcg cggacacgcg gccgatcatg ctgaatatgg
     1261 tggtgcgctc ggtaagccga gggtgccgga aggcgcccgc gaacagaccg acgccggtca
     1321 tatcgattgg gttgttgagc gtcgcggaat gcatgacgcg cgccacgcgc tcgaacggct
     1381 tcttcgttgc ggcatcgatg aagacgatgc ccaatccgtt cgctattttg tcgacggccg
     1441 gcgaattcgg atcgatgacg atgtcgcgca tgtgcgtgac ggccggcatt ccgggctcca
     1501 gcatcccgtg cagcaccgga gagttgaagt gaacgatgtc gtaggcgttg cgttcgagaa
     1561 ggtcgcgaaa gcggttgcgg tcgcgctgcc aactcagcca gtggacggta ccgtggagct
     1621 tatcggcaaa gctgcgtccg ataccaagca atcccaaatc gaccggcagc gaaagctcat
     1681 atacccgccg aacggaggga aagggtgccg cagtcgcggc cgcatcgcgc ggccggcgga
     1741 aggatcgcgg aaccagcaga tcggcttcga cgccggaata gtgttcgaga agcagccgca
     1801 agctcgtcgc cgctccgtaa cggctttcca gcgcgtgcgt gacgaacaag attttcttgc
     1861 tcattttgcg gacatttcac aggagcattg cccgaggcgg gcggctttca gcatcgtgca
     1921 tgccgcagga cgatttcgca aatccggtcg acgtcctcgt tgtccagctc cggatacatc
     1981 ggcaggctaa gaacccgtcc ggcgacgcgt tcggttaccg gcagcgaggc gggcttcgac
     2041 aggtccgcga aggccggttg gagatgcaac gggagcggat agtgaatgcc cgtctcgatt
     2101 ccctcttgcg ccagggcttt ggcaacctcc tcgcggttgg aggtttcgaa cacgtagagg
     2161 tgatagacgg gcgcgctgcc gtccgccggt tcgatagtct tgaacccggc gggccgcaac
     2221 cggccgtcgt aacgcgccgc cacgcgacgc cgcgcggcaa tccacgcgtc gagatggcgc
     2281 aatttcaccg acagtatcgc tccctgaagg gcgtccatac gctggttgta gccgacgatc
     2341 tggtgctcgt atttgctggt gcggccgtga tcgcgcagct tgcgcagcac agcggcggta
     2401 tcgtccgcgg cgcaggtaac gaagccggca tcgccataag cccccagatt cttgctggga
     2461 tagaagctga aggtgccgca gtctcccagc g
//
LOCUS       DASWPU010000386         8613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_40521,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000386 DASWPU010000000
VERSION     DASWPU010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8613
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40521"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VGC27_08315"
     CDS             complement(<1..511)
                     /locus_tag="VGC27_08315"
                     /inference="COORDINATES: protein motif:HMM:NF018037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HEY0282612.1"
                     /translation="MAIAIAAVPAHASLKLCNRTSYVLYAATAALETSGTFLRGWTRI
                     VPGSCRIAIQGDLAASAYYVYARTSPAHSGAPRAWNGNTNFCVKDTDFSLRLPLLTTR
                     CPAPDLYELPFAAIATHHMRSWTTTFRETPDFNSMKNAERAGLNRLLIDNGAKAGALG
                     SGSDKAAQAA"
     gene            complement(541..1467)
                     /gene="argB"
                     /locus_tag="VGC27_08320"
     CDS             complement(541..1467)
                     /gene="argB"
                     /locus_tag="VGC27_08320"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY0282613.1"
                     /translation="MALQDDLSQDRNMRVMARWRSTARILVEALPYILQYDGKIIVVK
                     FGGNAMTGTVAEDFAQDIVLMKQTGMEPVIVHGGGPQIGAMLKKLAIPSTFIDGLRVT
                     DEAAMEVVEMVLTGSINKRIVTGINAAGGHAVGLSGKDGNLVIARKIERVKIDPVTHE
                     SKAVDLGFVGEPETINPEVLRTFIKSDLIPVIAPVGVGRGGETFNINADTVAGAVAGA
                     MQAERLVLLTDVEGVFDQEGKLIPRLSLREARTLIANGTISGGMIPKIETAIDAVENG
                     VNAAVILDGRIPHVLLLELFTQHGAGTLITAE"
     gene            complement(1786..2439)
                     /gene="yihA"
                     /locus_tag="VGC27_08325"
     CDS             complement(1786..2439)
                     /gene="yihA"
                     /locus_tag="VGC27_08325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY0282614.1"
                     /translation="MSAGGEDEALAAARKLFAGPCDFVWGTGDIDSLPQQTLPEVTFV
                     GRSNAGKSSLINALTGRKTLARVSRTPGRTREINFFNLAGRLMLVDLPGYGYAKASKS
                     LAAEWQSLIFAYLRGRANLRRVVLLVDGRRGAMEIDRAVMDLLDGAAVSYCLALTKID
                     ELAPAERDTVLADAAAQARPHTAAYPEVFATSAHKGDGLDALKMQLAALAAPKSLSR"
     gene            complement(2436..4283)
                     /gene="yidC"
                     /locus_tag="VGC27_08330"
     CDS             complement(2436..4283)
                     /gene="yidC"
                     /locus_tag="VGC27_08330"
                     /inference="COORDINATES: protein motif:HMM:NF002353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY0282615.1"
                     /translation="MNDNRNLFVAIALSALLLVGWQYFVAAPQLKVEQARQAQLAQQQ
                     KKPQEPPSVVAPEPVNTAPEAVRSAPAKLSRAEALKRGGARVEIQTPTVDGSFLLKGA
                     RFDDLKLRKYHETVDPKSPEIELLAPSGSAYPYFAEFGWSAPGTKTALPNDQTPWQLK
                     SGTVLAPGKPVTLFWNNGQGLLFTRTITVDEQYMFTVTDSVENKSGAKTVLYPYALVV
                     RDGVPSHTSSYLLHEGFVGVGNDKLKDPTYDDFKKDGTPPQTFESTGGWVGITDKYWM
                     AAVVPPQNEKFNGTFRATPYGEIKSYQANYSLSGRTLEPGQTITTTQRLFAGAKVVNT
                     LRSYEHSLGIQRFDLAVDWGWFIFLTQPLFWLLDKLNHYLGNFGLAIIMATVAIRLLL
                     FPLANASFKSMGKMKKVQPEMERIKKLYSDDKVRQQQEMMELYKREKVNPVSGCLPML
                     VQIPILWSFFKVMFVTIEMYHAPFYGWIQDLSAPDPTSFLNLFGLLPFTVPQTLPGIL
                     APIAFLLHVGIWPILMGMTQWVQFKMNPAPPDPTQAKIFGLMPVVMTFMFAAFSSGLV
                     IYYTWNNLLSMAQQGYMMKREGVPIHLFENLRPPAFVRKLLGTKAQADK"
     gene            complement(4291..4530)
                     /gene="yidD"
                     /locus_tag="VGC27_08335"
     CDS             complement(4291..4530)
                     /gene="yidD"
                     /locus_tag="VGC27_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY0282616.1"
                     /translation="MRRVAAVVLIAPIRFYRTFISPMLPPSCRFTPTCSQYAIEAIQT
                     HGALKGLALTVRRLVRCHPITWLGGSHGYDPVPPR"
     gene            complement(4548..4907)
                     /locus_tag="VGC27_08340"
     CDS             complement(4548..4907)
                     /locus_tag="VGC27_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY0282617.1"
                     /translation="MRNRADFLRAQRGVRKFALGLVLEACPTPEILSQAGRLRVGYTA
                     SRKIGGAVERNRAKRRLRAAAAAVLPLSGRERTDYVLIARPATLNRPFDGLLEDLIAA
                     VAAANQKLRETKTGARP"
     gene            complement(4954..5088)
                     /gene="rpmH"
                     /locus_tag="VGC27_08345"
     CDS             complement(4954..5088)
                     /gene="rpmH"
                     /locus_tag="VGC27_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY0282618.1"
                     /translation="MKRPYQPSRIVRKRRHGFRTRMATKAGRTILARRRAHGRKKLSA
                     "
     gene            5289..6641
                     /locus_tag="VGC27_08350"
     CDS             5289..6641
                     /locus_tag="VGC27_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0282619.1"
                     /translation="MLLLTVLYALACEVLIFVPAIGLYHRDLLGDHILSAELAIMPFT
                     EPGGESLPESLRQELLKHANADAVLLKRSHRRDFFQVGGVPSRIDRTIDLTSDSLLAD
                     MRNGMNCLLSGGLRELHIIAPTRIKGAQSIGIVLSEAPLRNALLVYAKRAMAAALFIS
                     GATAALVFASLYFFLVRPMRRITNAMVVFHENPEDSSRILAASHSAGEIGLAERELAA
                     MQRDLHGFLRQKTRLAALGAAVARIQHDLRNILATAQLASDRLAASEDPVVKRLTPSL
                     VASIDRAIALATNTLKFGRMEERLPQKQPVVLRDLIDEVAQAVLVSLPRIVLDNRVEA
                     GLLADADREQLFRLILNLVRNAAEAVAGRETGAVIAVSATHMNRCIQIDIADNGPGIP
                     KAVLGRLFEPFASAGRAGGTGLGLAIARDLARGHGGELSLVSTGPDGTVFRITVPDCG
                     "
     gene            complement(6739..8436)
                     /gene="ggt"
                     /locus_tag="VGC27_08355"
     CDS             complement(6739..8436)
                     /gene="ggt"
                     /locus_tag="VGC27_08355"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0282620.1"
                     /translation="MTFLRLPALVFLLLTALCPPVLAEPVARHHMAAAANPYAAKAAL
                     DMLRQGGSAVDAAIAAQMVLTLVEPESSGLGGGAFMLLWDKATKKITSFDGRETAPAS
                     ATPGMFLDANGNPRAHFDAIPGGLSVGVPGVVAMLELAHRKYGKLPWAKLFVPAIALA
                     EKGVPVGHKLAETLRAYPQMLAMPDIKRAFTKPDGTPLKEGDILKNPALAATLRTIAK
                     GGAKAFYTGKIARDIVEKVRHAPVNPGGMTLADLAHYRAKERAPVCATYRANRVCSMG
                     PPSSGGISVLQILGMLERFPSKALAPNSLGEIHLFSQASRLAFADRTTYLGDPAFVRV
                     PVRGLLDRSYLRARAHQIDPARDMGQAKPGKPPSIRTGFAPQTSPEHHGTSHLSIVDD
                     EGQVVSMTTTVEFVFGSEMTVGGFFLNNELTDFSFVPTRDGKPVANAPAPGKRPLSAM
                     APTIVFDRHGRFAIALGSPGGPAIIPYVAGTLVAMIDGRLSAPAAVALPHHVNMNGPL
                     FLEKSTALDALAPALVRMGYDVRSGSGEKSGLHIIERVKNGYIGAADPRRDGVAIGD"
BASE COUNT         1518 a         2620 c         2836 g         1639 t
ORIGIN      
        1 gcgcggcttg tgccgccttg tcggaaccgg aaccgagagc gccggccttg gcgccattat
       61 cgatgagcag acggttcaaa ccggcgcgct cggcgttctt catcgagttg aaatcgggtg
      121 tttcgcggaa ggttgtggtc caggagcgca tatggtgggt ggcgatcgcc gcgaaaggca
      181 gctcgtacag atccggcgcc gggcaacggg tcgtgagcag cggcaggcgc agcgaaaagt
      241 ccgtgtcctt gacgcagaaa ttcgtatttc cgttccaggc gcgcggagcg ccggagtggg
      301 ccggcgaggt tcgggcatag acgtaatagg ccgaggccgc gaggtcgccc tggatcgcga
      361 tgcggcagga acctggaacg atgcgcgtcc aacctcgcag gaaggtgccg ctcgtttcca
      421 aagcggccgt cgccgcgtag agcacgtagc tggtgcggtt gcagagcttg agagaggcgt
      481 gcgccggcac cgccgcaatg gcaatcgcca tggccgtggc acaggacgcg aacaggcgcc
      541 tcattcggcg gtgatcaagg ttcccgcgcc gtgctgggtg aagagttcaa gcagcagcac
      601 gtgcggaatg cgtccatcga ggatgacggc ggcattgacg ccgttttcga ccgcatcgat
      661 tgcggtttcg atctttggga tcatcccgcc cgaaatggtt ccgttggcga tcagggtgcg
      721 cgcctcgcgc agcgaaaggc gcgggatcag tttcccctcc tggtcgaata cgccttcgac
      781 gtcggtgagc agcaccaggc gctcggcctg catggcgccc gcgaccgccc ccgccactgt
      841 gtcggcgttg atgttgaagg tctctccgcc ccgcccgacg ccgaccggcg cgatgacggg
      901 aatcagatcc gatttgatga aggtgcgcag gacttcgggg tttatcgtct ccggctcgcc
      961 gacgaagccc agatcgacgg ctttgctctc gtgcgtcacc ggatcgatct tcacgcgttc
     1021 gatcttcctg gcgatgacca gattgccgtc cttgccggag aggcccacgg catggccgcc
     1081 cgccgcattg atgccggtga cgatccgctt gttgatcgat ccggtcagca ccatctcgac
     1141 gacttccatg gccgcttcgt cggtgacccg aagtccgtcg atgaaggtgg agggaatggc
     1201 gagcttcttg agcatggcgc cgatttgcgg tccgcccccg tgcacgatga cgggctccat
     1261 gcccgtctgc ttcatcagca cgatgtcctg cgcgaaatcc tcggcgacgg tgccggtcat
     1321 ggcgttgccg ccgaatttca cgacgatgat cttgccgtca tactgcagga tatagggcag
     1381 cgcctcgacc agaatgcgcg ccgtcgagcg ccagcgcgcc atgacgcgca tgtttctgtc
     1441 ctgactgaga tcgtcctgaa gagccatcgg cgcgcctttg ggaaaacggg gccttatagc
     1501 ctaagcttta gagcctgtct cggtaatcgt cacggccgcg acgctgccga ttttgctccg
     1561 acccggcagg tggagctggc gacagccagc gacggcgcgc aggccgatgc ggggacatcg
     1621 ggcaagcgcg gcgacaaacc gggcgggcaa aatcggcgcg tcccgaaggg tttcggcgaa
     1681 aatcgccggc tggtttgtcg aggcgctcga ccgtactacc cggtacgctc ttcgcgcctc
     1741 tcctgccatc cgggcgattt ttgcacgaaa cgcggctcat gaggattacc gagacaggct
     1801 cttaggcgcc gccaacgctg cgagttgcat tttgagcgca tccaggccgt cccctttgtg
     1861 ggcggatgtg gcgaagacct cgggataggc cgcggtatgc gggcgcgcct gcgccgcggc
     1921 gtccgccaga acggtgtcgc gttccgccgg cgcaagctcg tcgatcttgg tcaacgccag
     1981 gcagtaggac accgcggccc cgtccagcag gtccatgacc gcgcggtcga tctccatcgc
     2041 cccgcggcgg ccatccacca gcagcaccac gcgcctgaga tttgcccgcc cgcgcaaata
     2101 cgcgaaaatc aggctttgcc actcggcggc gaggctcttc gaggctttgg cgtagccata
     2161 ccccggcagg tccaccagca tcagccttcc ggccagattg aagaagttga tctcgcgggt
     2221 gcgtccgggc gtgcgggaga cgcgcgccag cgtcttgcgg ccggtcagcg cgttgatcag
     2281 gctggatttg ccggcgttcg agcggccgac gaacgtgact tccggcagag tctgctgcgg
     2341 caggctgtcg atatcgcccg ttccccagac gaaatcgcac ggtccggcga agagctttcg
     2401 cgccgcggcg agcgcctcgt cctcgccgcc ggcgctcact tgtcggcctg ggctttggtg
     2461 cccaagagct tcctgacgaa cgccggcggg cgcagattct cgaacaggtg aatcggaacg
     2521 ccttcgcgct tcatcatgta gccttgttgc gccatcgaga gcaggttgtt ccaggtgtag
     2581 tagatcacca gccccgacga gaaagcggcg aacatgaagg tcatcaccac cggcatcagc
     2641 ccgaagatct tcgcctgcgt cggatcgggc ggcgcggggt tcatcttgaa ctgaacccat
     2701 tgcgtcatgc ccatcaggat cggccagatg ccgacatgga gcaggaacgc gatcggggca
     2761 agaatgccgg gaagcgtctg cggcaccgtg aagggcaaga ggccgaacag attgaggaac
     2821 gaggtcgggt ccggcgcgga caggtcctgg atccagccgt agaacggcgc gtggtacatt
     2881 tcgatggtga cgaacatcac cttgaagaac gaccacagaa tgggaatctg gaccagcatc
     2941 ggcaggcagc cgctcaccgg attcactttc tcgcgtttgt aaagctccat catctcctgt
     3001 tgctggcgaa ccttgtcgtc gctatagagc tttttgatgc gctccatctc cggctggacc
     3061 ttcttcatct tgcccatgga tttgaaggag gcgttggcca gcgggaagag caacaagcgg
     3121 atcgcgaccg tcgccatgat gatggccagg ccgaaattgc cgaggtagtg gttgagcttg
     3181 tccagaagcc agaacagcgg ctgagtaagg aagatgaacc agccccagtc gaccgccaga
     3241 tcgaagcgct ggattccgag actgtgctcg tagctgcgca gcgtattgac caccttggcg
     3301 cccgcgaaca gcctctgggt ggtcgtgatg gtctggccgg gctccaacgt acgcccgcta
     3361 aggctgtaat tggcttgata ggacttgatc tcgccatagg gtgtggcgcg gaaggtgccg
     3421 ttgaattttt cgttctgagg cggaacgacg gcggccatcc agtatttatc ggtaatgccg
     3481 acccagccgc cggtggattc gaaggtttgc ggcggcgtgc cgtccttctt gaaatcgtcg
     3541 taggtcggat ccttcagctt gtcgtttccg acgccaacaa acccttcgtg cagcagataa
     3601 gaactcgtat gcgacggcac gccgtcgcgc accaccagcg catagggata aagcaccgtc
     3661 ttggcgcccg acttgttctc cacgctgtcg gtaacggtga acatgtattg ttcgtcgacc
     3721 gtgatcgtcc gcgtaaacag aagcccttgt ccgttattcc agaacagagt cacgggtttt
     3781 ccgggcgcga gcacggttcc gctcttgagc tgccacggcg tctggtcatt gggcagtgca
     3841 gtcttggtgc ccggggcgct ccagccgaat tcggcgaaat agggataggc cgaaccggac
     3901 ggcgccaaga gctcgatttc ggggctcttg ggatcgacgg tctcgtgata ttttctgagc
     3961 ttcagatcgt cgaaacgcgc gcctttgagc aggaacgaac cgtccaccgt cggggtctgg
     4021 atctcgacgc gcgctccgcc gcgcttcagc gcttccgcac gcgacagttt ggcgggggcg
     4081 ctacgaaccg cttcgggtgc ggtattgacc ggttcggggg cgacgacgct tgggggttct
     4141 tgcggttttt tttgctgttg cgcgagctgg gcttggcgcg cttgttccac cttcaattgc
     4201 ggtgcggcga cgaaatactg ccagccgacc agcaacagcg ccgacagggc aatggcaacg
     4261 aagagattgc gattgtcgtt catcgaattc ctatcgcggt gggacgggat cgtagccgtg
     4321 cgaaccgccg agccaggtga tggggtggca gcgcaccagc cggcggacgg tcaaggccag
     4381 accttttaac gctccgtgcg tctggatggc ctcgatcgcg tattgcgagc aggtcggcgt
     4441 gaaacggcag gacggcggca gcataggcga gatgaaggtg cggtagaagc ggatcggcgc
     4501 gatcaaaacg acagcggcga cccgccgcag gctcacatta tggtcgatca cggacgggct
     4561 cccgtcttgg tctcgcgtaa cttctgattg gccgcggcaa ccgcggcaat aaggtcttcg
     4621 agaagaccgt cgaaaggccg gttcagcgtg gctggcctgg caatcagaac ataatcggta
     4681 cgttcgcgcc ccgaaagagg cagaacggcc gcagccgccg cgcgtagtcg ccgtttagcc
     4741 cggttgcgct cgaccgcgcc gccgatcttg cggctggcgg tgtaaccgac ccgcaaacgg
     4801 cccgcttggg ataggatttc cggggtcgga caggcctcca gcaccaatcc caaggcaaac
     4861 ttgcgcacgc cgcgctgggc acgcaggaaa tcggctctgt tcctcatgcg gtcggcgcct
     4921 aaccgctcca cgagcccagg agtcgtcaag ccttcaggcc gagagcttct tgcggccatg
     4981 ggcgcgccgg cgtgccagaa tggtgcgtcc ggctttggtg gccatgcgcg tgcgaaaccc
     5041 gtgacggcgc ttgcgcacga tccggctcgg ctgatagggg cgcttcatgg ctggtctccg
     5101 ggcggcgggc tactgcaaaa agaggcgcgg ttctaagcat taggaccggg aactgtcaat
     5161 gagagccccc ggccgccctg ttttttgccg ccgggatagg gctttatggg gacatgcggg
     5221 ttaagggcct gtttcgcacg cttgtgggaa ggggctgggg gctggtggct cacagcctat
     5281 cggggcgctt gctgcttttg actgtgctct acgccctggc gtgcgaggtg ctgatcttcg
     5341 tgccggcgat cgggctctac caccgcgatc tattgggcga tcacattcta tccgccgaac
     5401 tggcgatcat gccttttaca gagccgggcg gggagagcct gcccgaaagc ctgcgccaag
     5461 agcttctcaa gcatgccaat gcggacgcgg ttctgctcaa gcgcagccat cggcgcgact
     5521 tcttccaggt cggcggcgtc ccctcgcgca tagaccgcac gatcgatctg acctcggact
     5581 cgctgctggc cgacatgcgc aacggcatga actgtctgtt gagcggtggt ctgcgcgagc
     5641 tgcacatcat tgcgccgacg cgaatcaagg gcgctcagtc gatcggcatc gtgctgagtg
     5701 aggcgccgct cagaaacgcg ttgctcgtat atgcaaagcg cgccatggcg gcggcgctgt
     5761 tcatctccgg cgcaaccgcc gccctggtgt ttgccagtct ctatttcttt ctggtgcggc
     5821 cgatgcggcg catcaccaat gcgatggtgg tgttccatga gaatcccgaa gactccagcc
     5881 gcatcctcgc agcgtcgcat agcgccggcg aaatcggttt ggccgaacgc gagctggccg
     5941 cgatgcaacg cgatctgcat gggtttctga ggcagaagac gcggctggcg gctctcggcg
     6001 cggccgtggc gcgcatccag cacgatctgc gcaatatcct cgccactgcg cagcttgcgt
     6061 ccgatcggct ggcggcgagc gaggatcccg tcgtcaagcg tctgacgccc agcttggtcg
     6121 cctccatcga tcgggccatc gcgctggcga ccaacacgct caagttcggc agaatggaag
     6181 aacgtctgcc gcaaaagcag ccggtggtgc tgcgcgatct gatcgacgag gttgcgcaag
     6241 cggttttggt atctttgccg cgtatcgtcc tggataatcg cgtcgaggcc ggtttgctgg
     6301 cggacgccga ccgcgaacaa ttgttccgcc tcatcctgaa ccttgtccgc aacgccgccg
     6361 aagcggtggc ggggcgcgag acgggcgcag tgatcgccgt gtcggcgacc catatgaacc
     6421 gctgcattca gatcgatatc gccgacaacg ggccgggcat tcccaaggct gtactcggaa
     6481 gactgttcga gccttttgcc tcggcggggc gcgccggcgg caccggcctt gggctggcga
     6541 ttgcgcgcga tctggcgcgc ggtcacggcg gcgaactgag cttggtctcg acaggcccag
     6601 acggaaccgt cttccgcatt accgttccgg actgcggcta gagcaaatca ccgtttattg
     6661 gaatcggtga tttgctccag ctatttattt tgtcgcgctt cttatccgaa aatcggttcc
     6721 cacttttcgg gaagcgctct aatcgccgat cgctacgccg tcgcgcctgg gatccgccgc
     6781 gccgatatag ccgttcttca cccgttcgat gatgtgcagg ccgctttttt ctccgctgcc
     6841 ggaacgcacg tcatagccca tccggaccag cgccggcgcg agggcgtcaa gcgcggtgct
     6901 tttttccagg aagagaggtc cgttcatatt cacatgatga ggcagggcca cagcggccgg
     6961 cgcggagaga cggccgtcga tcatcgccac cagcgtcccg gcgacatagg gaatgatggc
     7021 gggtccgcca ggcgagccca gcgcgatggc gaagcgccca tgtctgtcga acacgatggt
     7081 cggcgccatg gcgctcagcg gccgtttgcc gggagcgggc gcattggcca cgggtttgcc
     7141 gtcgcgtgtc ggcacgaagg agaaatccgt cagttcgttg ttgaggaaga agcctccgac
     7201 cgtcatttcg gacccgaaca cgaactcgac cgtggttgtc atggacacga cctgtccttc
     7261 atcgtcgacg atggacaggt ggctggtgcc gtgatgttcg ggcgatgttt gcggcgcaaa
     7321 acctgtgcgg atcgaagggg gcttgccggg tttggcctgc cccatgtccc gcgccggatc
     7381 gatctggtgc gcccgtgcgc gcagatagga gcggtcgagc aagccccgca cgggcacgcg
     7441 cacgaatgcc ggatcgccga gataagtcgt gcgatcggca aaggccagcc ggctcgcttg
     7501 cgagaataga tgaatttcgc cgagcgaatt gggcgccagc gccttggacg ggaaccgttc
     7561 cagcatgccc aggatttgca gtaccgagat gccacccgaa cttggcgggc ccatcgagca
     7621 aacgcggttg gcgcggtatg tggcgcagac gggcgcgcgc tccttggcgc ggtaatgggc
     7681 cagatcagcc agcgtcatgc cgcccggatt gacgggcgca tgacgcacct tttccacgat
     7741 gtcgcgcgca atcttgccgg tatagaacgc tttggcgccg cccttggcga tggtgcgcag
     7801 ggtggcggca agcgccggat ttttcaggat gtcgccttct ttgagagggg ttccgtccgg
     7861 tttcgtgaaa gcgcgcttga tgtcgggcat ggcaagcatc tgcgggtagg cgcgcaaggt
     7921 ttccgccagc ttgtgaccga ccggcacgcc cttctcggcg agcgcgatgg ccggcacgaa
     7981 cagtttcgcc caagggagct ttccgtattt tctgtgcgcc agttcgagca tggcgacgac
     8041 gcccggcacg ccgaccgaca atccgcccgg aatggcatcg aaatgagcgc gcgggttgcc
     8101 gttcgcgtcc aggaacatgc cgggcgtggc cgacgccggc gcagtctcgc gcccgtcgaa
     8161 actggtgatc ttctttgtcg ccttgtccca cagcagcatg aaggcgccgc cgcccaaccc
     8221 ggaggattcc ggctcgacca gcgtcaagac catctgggcc gcaatagcag catccacggc
     8281 cgatccgccc tggcgcagca tgtcgagagc agctttggcg gcatatggat tggcggccgc
     8341 agccatgtga tgcctggcga cgggctccgc cagcaccggc ggacacaaag ccgtcagcaa
     8401 caggaagacc agggccggca aacgcaggaa tgtcatcgcg tctctctttt cgggaggcat
     8461 gccaaaggct agcgtcgtgc gcatgatacg caagggagtt cgcaacctta ttacggatgt
     8521 tgccggactt cgcgtgggcc aggccgagga cgttgccgcg cgcacgggcg tcacggtgct
     8581 gctgcgcgac gagcctgcgg ccgcggccgc cga
//
LOCUS       DASWPU010000387         3957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_71037,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000387 DASWPU010000000
VERSION     DASWPU010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71037"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VGC27_08360"
     CDS             complement(<1..353)
                     /locus_tag="VGC27_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0282621.1"
                     /translation="MDRIPLVDLKKQYESIKLEILAAIDEVFRAGVFIQGPPVRDFET
                     AFAQKLGAAHGIGVANGTAALSLALEALGIGRGDEVITVSHTFIATAEAICHVGATPV
                     FVDINQGDYCMDPGA"
     gene            complement(524..1561)
                     /locus_tag="VGC27_08365"
     CDS             complement(524..1561)
                     /locus_tag="VGC27_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0282622.1"
                     /translation="MLGFALVGCGRIAQKHLKLLSEGRIQHSKLVAVCDILPHIARES
                     GERYGVPWFTDMHQMMQKTAAQADVVSILTESGNHAAHCLALAPYRKHIVVEKPIALA
                     VEDADEMVRACDAAGTKLFVVKQNRYNVPVQKLMEAYRQDRFGKLVMGTVRVRWCRKQ
                     AYYDQAAWRGTYALDGGVFANQAAHHIDLLQWFLGKPLSVVAKARTALVDIEAEDTGV
                     AVITFEGGAIGVVEATTAARPKDTEGSLSVLGEKGLVEIAGFAVNHVRTWNFADERPE
                     DKDALSKFSENPPDVYGFGHVAYLNHVVDVIRNGAPSLVDGLEAKKSVELICAIYESI
                     RSGSEVQLHRR"
     gene            complement(1672..2145)
                     /locus_tag="VGC27_08370"
     CDS             complement(1672..2145)
                     /locus_tag="VGC27_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEY0282623.1"
                     /translation="MEALALEDWESFYRRIDYFLVCGCGLLDGRFCTSARIINCHPGL
                     IPQTRGLDAFKWCIYKGRPLGVTLHRIDERVDLGTVLHHEPTDVFEEDDLATLAARHY
                     NAEVDLVANFDRYLKGGTILPLEIQDATKRMPLEVEREMLRNFETFKRRSTGGAN"
     gene            2570..3781
                     /locus_tag="VGC27_08375"
     CDS             2570..3781
                     /locus_tag="VGC27_08375"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0282624.1"
                     /translation="MVPRFDPADIEAVRHRHFLASSRWKNEDEKGILPDDEYGAPVSP
                     SDAASRTESSGQPRFSAASVAQNRIRDERRRGSIRLEDNIMKQALVCGAGGFIGSYVV
                     KRLRREGFWVRGVDLKFPEFSETAADDFVIGDLRDPDVCNLIVDRHFDEVYQFAADMG
                     GAGYIFTGEHDADVMRNSALINLNMIETCFKRNIKRVFYSSSACIYPAHNQEDADNPN
                     CTEGSAYPAAPDSEYGWEKLFSERLYLAYNRNHGMECRIARYHNIFGPEGTWTGGREK
                     APAALCRKVAMATSGDTIEIWGDGLQTRSFLYIDECLDGTLRLARSEHPGPFNIGSEE
                     MVSINQLAEMVMDIAGKKLRIEHIPGPLGVRGRNSDNTLIKATLGWAPSKTLREGLEK
                     TYAWIEAQVHH"
BASE COUNT          827 a         1141 c         1178 g          811 t
ORIGIN      
        1 aaggcgccgg ggtccatgca gtaatcgcct tgattgatat cgacgaagac gggcgtcgcg
       61 ccgacatggc agatcgcctc cgccgttgcg atgaaggtgt gggatacggt gatgacttcg
      121 tcgccgcggc cgatcccaag cgcctccaag gccagcgaca gggccgccgt tccattcgca
      181 acgccgatgc cgtgagccgc gcccagtttt tgcgcgaagg cggtttcgaa atcccttacc
      241 ggcgggcctt ggatgaacac gccggcacga aaaacctcgt cgatcgccgc gagaatttcc
      301 agtttgatcg attcatattg ctttttcagg tcgacaagcg gaatgcgatc cattggaacc
      361 ccgtgctgct cacttggccc ggcaagtggg atatcttcgt cgacggccca agatgacatg
      421 gtgccgtgca ggcggcaagc caacggaacc gtcgctcgtt cgtcttgaat atcgcggtgc
      481 gtttggcgct tttccggcac gcaaccgcat cggcgccgcc ggtctaccga cgatgaagtt
      541 ggacctccga tcctgatctg atcgattcgt aaattgcaca aatcaactcg accgacttct
      601 tcgcttcaag cccgtctacc agggaaggcg ctccattccg tatgacgtcg actacgtggt
      661 tgagataggc gacatggccg aagccgtaga catcgggcgg attctcggaa aacttgctca
      721 aggcgtcttt gtcttccggc cgttcgtcgg cgaagttcca cgtccgcacg tgattcacgg
      781 caaagccggc gatttccacc aggccttttt cgccgaggac ggaaagcgat ccttccgtgt
      841 ccttcgggcg cgccgccgtg gtggcctcga cgactccgat cgcaccgcct tcgaaggtga
      901 tgacggcgac ccccgtatcc tcggcttcga tatcaaccag cgcggtgcgc gccttggcga
      961 ccacggaaag aggcttgccg agaaaccatt gcaggagatc gatgtggtgg gccgcctgat
     1021 tggcgaagac gccgccatcg agcgcgtagg tgccccgcca agcggcttga tcgtaataag
     1081 cctgtttacg acaccagcgc acgcgaaccg tgcccatcac caacttgccg aaacggtcct
     1141 gccggtatgc ctccatgagt ttctggaccg gcacgttgta gcggttctgc ttgaccacga
     1201 acaatttcgt gccggccgcg tcgcaggcgc ggaccatctc gtcggcgtcc tcgaccgcca
     1261 atgcgatcgg cttctcgacc acgatgtgtt tgcggtaggg ggcgagcgcc aggcaatgcg
     1321 cggcgtgatt tccgctttcc gtcaggatgc tgacgacatc cgcctgggcc gcggtttttt
     1381 gcatcatctg gtgcatgtcc gtgaaccaag gaacgccgta gcgctcaccg gattcgcgcg
     1441 cgatatgcgg cagaatgtcg cataccgcca caagcttcga atgctgaatg cggccttcgg
     1501 aaaggagttt gagatgcttc tgggcgatcc ggccacagcc gaccaaggca aaacctagca
     1561 tcacacccag ccagctcttt gaggcatctc agtcgaaggc gcggccgacg attgaaccgc
     1621 gctcccctaa tatcgatagc ggtacggttt gaatagggca aagtggtttt cttagtttgc
     1681 tccgcccgta ctgcgacgtt tgaaggtttc gaaatttcgc agcatttccc gctcgacttc
     1741 gagcggcatg cgcttggtgg cgtcctgaat ttcgagcgga aggattgttc cacccttaag
     1801 atagcggtcg aaattggcga ccaaatcgac ttccgcgttg taatgtctgg cggccagggt
     1861 cgccaaatcg tcttcctcga agacatcggt cggctcatga tgcagcacgg ttccgaggtc
     1921 tactctctcg tcaattcgat gaagcgtgac gcccagcggg cggcctttgt aaatgcacca
     1981 tttgaaggcg tccaagccgc gggtctgagg gatcaatccg ggatggcagt tgatgatccg
     2041 tgcgctggtg caaaagcggc cgtcaagcag accgcagccg cagaccagga agtagtctat
     2101 gcgccgatag aaggattccc agtcttcgag ggcaagcgcc tccaaattat atttccgcgc
     2161 gagactgtga ggaccgggcc cagtaagctg gttgggccgg tgttgaaagg ccgtcgggcg
     2221 ttgcggtcgc ggcttgaaag ccaccaaagt caaactgagt tcaaattctt ttcgccggag
     2281 cattcgaaga agaacctcct gcgtcttctg atgtggcgcg tcgtaggtta ttagactgac
     2341 tcgcatctca acacctcacc ccatgcggtc cggttgcacg atcctcggga acggccctgt
     2401 tggcggcctc ccgaacttcc cgatggttca ccatatcaga catttcccga cgcttggacg
     2461 aggcctccaa ggccgtttgg cgaggcagcg gatataattg cggagggcgg ccgttgccgc
     2521 cagacgatat cgggcatcgc gcggtatttg ggaaagcggc aacgccgcaa tggtaccgcg
     2581 cttcgatccc gccgatatcg aggctgtccg ccaccgtcac tttcttgcat ccagccgctg
     2641 gaagaacgag gacgagaagg gtatcttgcc agatgacgaa tatggcgcgc ccgtttcccc
     2701 gtctgatgct gcctcaagaa cagaatcaag cggccaaccg cggttttccg ctgcatccgt
     2761 cgcccaaaat cgcattcgcg acgaacgaag acggggcagc atccgattag aggacaatat
     2821 tatgaagcag gcgcttgtgt gcggagccgg cgggttcatc ggcagttatg ttgtgaaacg
     2881 cttaagacgt gaaggctttt gggtgagggg cgtcgatctg aaatttccgg aattttcgga
     2941 aacagcggca gatgactttg ttattggcga tctgcgcgat ccggatgtgt gcaatttgat
     3001 cgtcgataga catttcgacg aagtttatca gttcgcggcc gatatgggcg gagccgggta
     3061 catcttcacg ggggagcatg acgccgacgt gatgcgcaat tcggctttga tcaatctcaa
     3121 catgatcgag acctgcttca agcggaacat caagcgggta ttctattcct cttcggcatg
     3181 catttatccc gcccataacc aggaagacgc ggacaatccg aattgcaccg agggctccgc
     3241 ttaccccgcc gcgccggaca gcgagtatgg ctgggagaaa ttgttcagcg aacgtcttta
     3301 cctggcctac aaccgcaacc acggcatgga atgccgaatc gcgcgctacc acaacatatt
     3361 cggccccgaa ggcacgtgga ccgggggcag agaaaaagcg cccgccgcgc tttgccggaa
     3421 ggtcgcgatg gcaacgagtg gagataccat agaaatctgg ggcgacggcc ttcaaacgcg
     3481 ctcgttcctt tatatcgacg aatgcctcga tgggaccctt cggttggctc gctcggaaca
     3541 tcccggccct ttcaacatcg gctcggagga gatggtcagc atcaatcaat tggccgagat
     3601 ggtgatggac attgccggca agaaacttcg tatcgagcat attcccggtc cgctcggcgt
     3661 acgcggccgc aactccgaca acacgctgat caaagccacg ctgggctggg ctccgagcaa
     3721 gacgctacga gaaggcctgg aaaagactta tgcgtggatc gaagcgcagg tgcaccattg
     3781 aaagggtagc gcgccgcaat ctgaaaggta ctcagcgacc gccgccgtgg cccggcgcgc
     3841 gaggaagcgc cgcgctgagt tcgcgagcaa gagcgatggc aagacgccgg cctgtcggcg
     3901 gagtaacgac ggcgattgcg gacggcggcc atagtagtca gaatatatcg tgcatcg
//
LOCUS       DASWPU010000388         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_64928,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000388 DASWPU010000000
VERSION     DASWPU010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64928"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VGC27_08380"
     CDS             <1..545
                     /locus_tag="VGC27_08380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY0282625.1"
                     /translation="GMEGALRRRGDVVNAPAAEQLGVADLDALRALRLEALALHPDCF
                     CADPDLEEARTAEQWKEALRRGTWFGVRKDGALAAIAAFTRPQSKKTGHTGELASMYV
                     RSEARGSGIADALVRRIVEHAAGEVDQIKLAVNADNARAIKLYERHGFRIVGRIPRYI
                     RVGDRLHDEVIMLRGVSPSD"
     gene            complement(558..1298)
                     /gene="bla"
                     /locus_tag="VGC27_08385"
     CDS             complement(558..1298)
                     /gene="bla"
                     /locus_tag="VGC27_08385"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B2 metallo-beta-lactamase"
                     /protein_id="HEY0282626.1"
                     /translation="MKFLAGFLVALTSASALAAEPALTLHQLRGGVYVVEDGYYAKEN
                     SAVYIGRDHVTVVGATWSPETARLLAAEIGKITPKPITEVINTNYHPDRAGGNAYFKS
                     IGAKIIATEMTRDLMRSGWDGVIAWTRNAIPDYPALPPVLPDTVYPGGFALQDGRVRA
                     IYLGPSHTPDGIFVWFPEEKVLYGGCILKEQLGNLDYADLAEYPATLRKLQRLDLGFT
                     AVIAGHYSSVHGPELVGRYIRMLEQRPP"
     gene            complement(1324..>1839)
                     /locus_tag="VGC27_08390"
     CDS             complement(1324..>1839)
                     /locus_tag="VGC27_08390"
                     /inference="COORDINATES: protein motif:HMM:NF016277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282627.1"
                     /translation="IVTDGFELSRNQKRITRRNADLLRSVVPAEATREQFALLRTYLD
                     SRHAGGGMSDMGLFDYVAMVEETPVNTHIVEYRKPDPDGEPGQLVACALTDVLRDGLS
                     MVYSFYHPGEDARSLGTYMILDHVRAVQMRTQPHVYLGYWVRGSAKMDYKSRFRPLEA
                     LTRDGWIRAPD"
BASE COUNT          336 a          570 c          606 g          327 t
ORIGIN      
        1 gcggaatgga aggcgcgctt cggcggcgag gagacgtcgt gaacgcgccg gcggccgaac
       61 agctcggcgt cgccgacctg gacgctctgc gcgccctccg gctggaagcg ctggccctgc
      121 atccggactg cttctgcgcc gatcccgacc tcgaagaagc gcgaaccgcc gagcaatgga
      181 aggaagccct gcggcgcgga acctggttcg gcgtgcgcaa ggacggagca ctggccgcca
      241 ttgccgcttt cacgcgcccg cagtccaaga agaccggcca taccggggag ctggcgtcga
      301 tgtatgtgcg ctccgaagcg cgcggttcgg gcatcgcgga tgcgctggtg cgcaggatcg
      361 tcgaacatgc ggccggcgaa gtcgatcaga tcaagctcgc cgtcaatgcc gataatgccc
      421 gcgccatcaa gctctacgag cggcacggtt tcaggatcgt cgggcgcatc ccacgctata
      481 tccgcgtagg cgaccggctg cacgacgaag tgatcatgct gcgcggcgtg tcgcccagcg
      541 attgatcgcg gcgcgtttca gggcgggcgc tgctcaagca tccggatgta tcggccgacc
      601 aattccgggc cgtggacgga cgagtaatgg ccggcgatga cggcggtgaa gccgaggtcg
      661 agccgctgca atttgcgcaa cgtcgccgga tattcggcga ggtcggcata gtcgagattg
      721 ccgagttgtt ccttcaggat gcagccgccg tataaaacct tctcttcggg aaaccaaacg
      781 aagataccgt ccggcgtatg cgacggccca agataaatcg cccgcacccg gccgtcttgc
      841 agcgcgaagc cgccgggata aaccgtgtcg ggcaaaaccg gcggcaaggc gggatagtct
      901 ggtatcgcat tgcgcgtcca tgcgatcacg ccgtcccagc cggaccgcat gaggtcgcgc
      961 gtcatctcgg tggcaatgat tttggcgccg atgctcttga aataggcgtt tccgcccgct
     1021 cggtcgggat ggtaattcgt attgatcact tcggtgatcg gcttgggcgt gatcttgccg
     1081 atctcggctg ccagcaatct ggcggtttcg ggcgaccagg tggcgcccac cacggtcacg
     1141 tgatcgcggc cgatataaac cgccgaattc tctttggcgt aatagccgtc ttcgacgaca
     1201 tagacgccgc cgcgcagttg atgaagcgtg agcgccggtt ccgccgccag cgcggaggct
     1261 gatgtcaacg ccacgagaaa gccggccagg aacttcatcg gcgtcaccgc atttgggcaa
     1321 tttttaatcc ggcgccctta tccagccgtc gcgcgtcagc gcttcgaggg ggcggaagcg
     1381 ggacttgtaa tccatcttcg cgctgccccg cacccagtag ccgagataga catgcggctg
     1441 ggtgcgcatc tgcaccgccc ggacatgatc caggatcata taggtgccca ggctgcgcgc
     1501 gtcttcgccg ggatgataga agctgtagac catcgacagg ccgtcgcgca gcacgtcggt
     1561 cagcgcgcag gcgacaagct gtccgggctc gccgtccggg tccggcttgc gatactcgac
     1621 gatgtgggtg ttgacgggcg tctcttcgac catggccaca tagtcgaaca ggcccatgtc
     1681 ggacatgcct cctccggcat gacgcgagtc gagataggtg cgcagaagcg cgaattgctc
     1741 gcgcgtggcc tcggccggaa ccacgctgcg aagcaggtcc gcattgcgcc gggtgatgcg
     1801 cttttgattg cgcgaaagct cgaacccgtc cgtcacgat
//
LOCUS       DASWPU010000389         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_64933,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000389 DASWPU010000000
VERSION     DASWPU010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64933"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..1182
                     /locus_tag="VGC27_08395"
     CDS             466..1182
                     /locus_tag="VGC27_08395"
                     /inference="COORDINATES: protein motif:HMM:NF017672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HEY0282628.1"
                     /translation="MTSQPQFLGPFYCETGYPWLGMAEPVNTITNAAIIVAAYFAYRA
                     VKKSRVGFSADLVLLIFLLAMVGIGSTLWHGLRTRWALQLDWIPGVLFLLVLTALWFR
                     QLFGWFAGIGGAILMYAATVASAAFAWRTFGDLHGAATNLRFVPGFAVITLFGLALVA
                     ATRRKFGREAAVQGVIILSCGIGAAVARSIDLLVCPYVPIGTHFLWHLLLSTASFLGI
                     LLLVRMKKQRRAVLALSEMG"
     gene            1386..1676
                     /locus_tag="VGC27_08400"
     CDS             1386..1676
                     /locus_tag="VGC27_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY0282629.1"
                     /translation="MNVVTYSYARQNLAKLMDEVERDRAPIHISRQRTRGAAVLVSQD
                     EWSSIQETLYLLSNPENARLLRKGIAELDAGKGIEFDPAKSARKPSKRNRRK"
     gene            1673..1942
                     /locus_tag="VGC27_08405"
     CDS             1673..1942
                     /locus_tag="VGC27_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEY0282630.1"
                     /translation="MKIVWAPDALKDYRELRDTAPALADKIDRLIDDIRRQPFAGLGK
                     PEPLRGEFAGWWSRRVGRGHRLVYRVAGKRGSDQRLEILQCRFHY"
     gene            complement(2084..>3528)
                     /locus_tag="VGC27_08410"
     CDS             complement(2084..>3528)
                     /locus_tag="VGC27_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY0282631.1"
                     /translation="RGTIVAFKPDRKIFGDDIHFSPVRLYRMAKSKAYLYRGVEIRWK
                     CAESLIDNGTPAEDTLKFPGGLTDFLAGEIEGKKTVTPRTFFGATSNRDGKGSVEWAV
                     AWAPEAEPFLHTYCNTIPTLQGGTHEQGLRNALTRSLRAHGERANNRKTALITPDDLM
                     ASACAILSIFIREPEFQGQTKEKLSSPDAQRLVENVVRDHFDHWLAESPQDADRLLDF
                     VIDQAEERLKRKQEKEVSRKAATRKLRLPGKLADCTRDAAAGTEIFLVEGDSAGGSAK
                     QARDRNTQAILPLRGKILNVASAAAGKLAQNQELSDMVQALGCGVGAKYREEDLRYER
                     VIIMTDADVDGAHIASLLLTFFYREMPQLIAHGHIFLAMPPLYRLSHGGTTIYARDDA
                     DRDRLLKSAFKANAKVEVSRFKGLGEMMPAQLKETTMKPGARILVRVEIADDRRKETE
                     NLVERLMGKKPELRFQFIQENAQFAKDVDV"
BASE COUNT          618 a         1068 c         1104 g          738 t
ORIGIN      
        1 accaagccta tgcacactgc agccaagcca acgcacacga gcccaaggaa tacccgcggt
       61 atccacgaag gctcgatata cagattttgg aggctgacag agagatagag ccgctcttcc
      121 gctgtttgct tccatgcgaa ccgtatcagc gccgctatcg ctgccacgat cagtaacctc
      181 actggtccgg atatacgcaa agggctgctc caacccattc acaccttcac aatatcacca
      241 atctcccatt ttcgtcgtgg ccgggcttga cccggccatc cagagtcttt ttgcgcgcgg
      301 acgcgcgttt gctggatggc cgcctgcttg cactccggcc gcgcttcgcg cggagcgggg
      361 tagcggccat gacgaagtaa ggagtgaggt ggttcagaga cttccgctgg acgccgagcg
      421 ccaggcgcgg caatgtccgt tgccgaagta tgagcggacg cacgcatgac atcccagcct
      481 caattcctcg ggccttttta ttgcgaaacc ggatatccct ggctcggcat ggccgagccg
      541 gtgaatacga tcaccaatgc ggcgatcatc gtcgcggcct atttcgccta tcgcgccgtg
      601 aagaaatccc gcgtggggtt ctcggccgat ctggtgctgc tgatcttcct gctcgctatg
      661 gtcggcatcg gctcgacgct gtggcacggc ttgcgcacca ggtgggcgtt gcagctcgat
      721 tggattccgg gcgtgctgtt tcttctcgtg ctcacggcgc tgtggtttcg ccagctcttc
      781 ggatggttcg cgggcatcgg cggcgcgatc ctgatgtatg ccgcgacggt cgccagcgcg
      841 gctttcgctt ggcgcacttt cggcgacctg cacggcgcgg cgacgaatct gcgcttcgta
      901 ccggggttcg cggtcatcac cttgttcggc cttgccctgg tggccgcgac gaggcgcaaa
      961 ttcggccgag aggcggcagt gcaaggcgtg atcatcttgt cgtgcgggat cggcgcggcg
     1021 gtggcccgtt ccatcgatct tctcgtctgt ccttatgtgc cgatcgggac gcatttcctc
     1081 tggcacctgc tgctctccac cgcctctttc ctggggatat tgctgctggt gcggatgaag
     1141 aagcagcgcc gggcggtgtt ggctttgtcg gagatgggat gacaaagagc gagtagcgaa
     1201 tagcgagtag ggaccctatt cgctattcgc tattcaacgt ccacatcctt cgcgaactgg
     1261 gcgttccggc atctgctgct gtccacggta tcgttccttg agattgtgct ggtggcggga
     1321 atgaagacgc agaacttgac aaacggttgg ctattgtaca acatattgta caataggagt
     1381 tgcacatgaa cgttgtcacc tacagctatg cccgccagaa cctcgccaag ctgatggacg
     1441 aggtcgagcg cgaccgcgcc cccattcata tttcgcggca gcgcacccgt ggcgcggccg
     1501 ttctggtctc acaggacgag tggagttcca tccaggaaac tctgtatctc ttgagcaatc
     1561 ctgaaaacgc gcgcctgctg cgcaaaggca tcgcggaact cgatgcaggc aagggcatcg
     1621 agttcgatcc ggccaagtcc gcccgcaagc cgtcgaagcg caatcgacgc aaatgaaaat
     1681 cgtctgggct cccgacgcct tgaaggacta tcgcgaactg agggataccg ccccggcgct
     1741 cgccgacaag atcgaccgcc ttatcgacga tattcggcgg caaccatttg ccgggttggg
     1801 caaaccagaa ccattgcgag gcgaattcgc gggctggtgg tcgcgacgcg tgggacgggg
     1861 acaccgtctg gtctatcgcg tggccggcaa aaggggctcc gatcaacgcc tcgaaatcct
     1921 gcaatgccgg tttcattact gacatgccgc tcgataagct accggacgca tttcctctgg
     1981 cgcttgctgc tggtgaagat gaataaggcg aatagcgaat agggaacaac aaacagtttc
     2041 tactccctac tccctactcc ctactcccta ttcgctattc gccctaaacg tccacatcct
     2101 tcgcgaactg ggcgttttcc tgaatgaact ggaagcgcag ttcgggcttc ttgcccatca
     2161 ggcgttcgac caggttctcg gtttccttgc gccggtcgtc ggcgatctcg acgcggacca
     2221 ggatgcgcgc gccgggcttc atcgtggttt ccttgagctg cgccggcatc atctcgccca
     2281 gacccttgaa gcgcgagact tccaccttcg cattcgcctt gaacgccgat ttaagcaggc
     2341 ggtcgcggtc ggcgtcgtcg cgggcataga tcgtggtgcc gccatggctc aggcgataaa
     2401 gcggcggcat ggcgaggaag atgtgcccgt gcgcgatcag ttgcggcatc tcgcgataga
     2461 agaaggtcag cagcagagag gcgatatggg cgccgtccac gtcggcgtcg gtcatgatga
     2521 tgacgcgctc gtagcgcaaa tcctcctcgc ggtatttcgc gcccaccccg cagcccagcg
     2581 cctgcaccat gtccgacagt tcctggttct gcgccagctt gcccgccgcc gcgctggcga
     2641 cgttaaggat tttcccgcgc aagggcagaa tcgcttgggt gttgcggtcg cgcgcctgct
     2701 tggccgagcc gcccgcggag tcgccttcga ccagaaagat ttccgtgccg gccgcggcgt
     2761 cgcgggtaca gtccgccagc ttgccgggca ggcgcagctt gcgcgtcgcc gccttgcgcg
     2821 agacttcctt ctcctgcttg cgcttgaggc gctcctcggc ctgatcgatg acgaaatcga
     2881 gcaggcggtc ggcgtcctgc gggctttccg ccagccaatg gtcgaaatga tcgcgcacca
     2941 cgttctcgac cagacgctgc gcatcgggcg aggagagctt ttccttggtc tggccctgga
     3001 attccggctc gcggatgaag atcgacagga tggcgcaggc cgaggccatc agatcgtcgg
     3061 gcgtgatcag cgcggtcttg cggttgttgg cgcgttcgcc gtgggcgcgc agggagcgcg
     3121 tcagcgcgtt gcgcaagccc tgttcgtggg tgccgccttg cagggtgggg atggtattgc
     3181 agtaggtatg caggaacggt tcggcctcgg gcgcccaggc gacggcccat tccaccgaac
     3241 ctttgccgtc gcggttggag gtggcgccga agaaggtgcg cggcgtgacg gtcttcttgc
     3301 cttcgatctc gccggcgaga aaatcggtaa ggccgccggg gaatttcaac gtgtcttcgg
     3361 cgggggtacc gttgtcgatc aggctctcgg cgcatttcca gcggatttcg acgccgcgat
     3421 agagataggc cttggacttc gccatgcggt aaagccgaac cggcgagaag tggatgtcgt
     3481 cgccgaaaat cttccggtcg ggcttgaagg cgacgatggt gccgcgcc
//
LOCUS       DASWPU010000390         4870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_9037, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000390 DASWPU010000000
VERSION     DASWPU010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9037"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2029
                     /locus_tag="VGC27_08415"
     CDS             <1..2029
                     /locus_tag="VGC27_08415"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY0282632.1"
                     /translation="DKLNREHVRSYLEHYLDMYQEVTGPALFGRRGLQALMVDSAEIG
                     EQNWTENILAQFKRLHGYDATPWLPALTGVVVASPAESDKFLWDFRRTIAELIARDHY
                     GEISKIARERGLIDYGEALEDHRPGFGDDMEMRQFAAIPTGAMWTYGKEAGPQPTYVA
                     DLLGAASVAHIYGRPLVAAESLTSAGQPWAFAPRELKPVVDAEFALGVNRVVIHTSVH
                     QPIDKPPGLSLFGYGQFFNRLESWAQEARPWITYISRCSYLLQQGRYVADVAYFYGQE
                     APITGEFGTNPVADVPQGYAFDFANSDALLNKFSVDNGDLVTPSGMRYRVLYLGGSSR
                     LMTLSVLRRIRDLVEQGATLVGKRPQSSPSLKDDPAAFQKTADELFGGADEHSYGKGR
                     VFVSGSLPEALAALNLPPDFEYTKPQADTELLALHRRLTDGELYFVSNRHDRVENVTA
                     TFRVTGYSPEIWNAVTGEIAKANFTAADGRTKVSLSLPAYGSAFVVFRDKTEAAAKIA
                     PASAVATLATLKGPWKISFLPGRGAPAAITQRVLESWSDSNIPGVKYFSGTATYTNTF
                     VLPKAVLKNGTRLVLDLGEVRELAEVSLNGKAVGTVWTAPFQLDITKAVKPGKNALKI
                     KVVNLWVNRLIGDAQPENKKKYTFTTIPTYKPDAPLRESGLLGPVVVRRVN"
     gene            2043..>4870
                     /locus_tag="VGC27_08420"
     CDS             2043..>4870
                     /locus_tag="VGC27_08420"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY0282633.1"
                     /translation="MQSSVGKRLLPLLLCSVVFVGSCLAEPPAKSDALLSGFRNPPAS
                     ARPGVGWPWMNGNVTIEGIDKDLSWMHRVGITSVGIGSAAIDTPTVVKKRLEYLTPEW
                     KEAFRYAVGEADKYGIQVGIGAAAGWSMTGGPWVTVQQSMKKLVWSATSVEGGKPFHG
                     VLPQPPDVIGPIQNAPWFGDHAPTQQTANLHFYRDAIVVAYRDTVTAPVVASATASTG
                     KLNANILDDGDLSKGVILEPATEDGDVWVQVKYKKPATIQGVTLGLIVPKELGYHVVV
                     EASNNGGDWRHVADFPQVAQLQRMQMGEQTISFAPVTARYFRLVLKATPPIPTSYRPK
                     KGDAAPGMIPAEAEVTPHRAYDVREVAFRAGATVNEFEKKAMFAAPPRDFYTINSVPD
                     TAPGTAIDPSSVVVLSDKMKPDGTLDWTPPPGKWVVMRLGYSPVGKENHPAPPEATGL
                     EVDKLNGAHVRHYLERYIDFYKTITGPELIGKRGVTSIGLGSSEISEQNWTENIISEF
                     KRLRGYDPSPWLPALTGLVVKAPADSDKFLWDWRQTVEDLFKENHYGMFCQVMHEHDL
                     KCGGQALEDHRPTFGDDMDMRQYFDSVGAAMWTYDSKKFPAILAYEADIQGAASVAHI
                     YGANLVNLESLTSDRQPWWWAPRDTKKFIDLEFALCGNRLGIHTSVHQPVDKPPGLSL
                     GPFGQFFNRHETWAEMARPWLDYIARASWMLQQGQYVGDIVYFYGQEAPIVSLWGNKR
                     VEGVPQGYAFDFANTDVLLNRLSVENGALVTKTGMRYRVLYLGGSSDKMTLTALKKVR
                     KLVEQGAVIVGKRPVDTPSLVDDPAQWQAAADAVFGKPGETSVRALGQGKVIASGSLE
                     EAFAELKVAPDFSYTGTDAQLLYTHRHLKDGDVYFVSNRENKPVTASASFRVAGRTPQ
                     LWDAITGKISPVGYRIEDGRTIVNLALPA"
BASE COUNT          981 a         1482 c         1517 g          890 t
ORIGIN      
        1 ggacaagctc aaccgcgaac acgtccgcag ctatctggaa cactatctcg acatgtacca
       61 ggaggtgacc gggcctgcgc tgttcggacg gcgcgggttg caggccttga tggtggacag
      121 cgcggaaatc ggtgaacaga attggacgga gaatatcctt gcccagttca agcgtctgca
      181 cggatacgat gccaccccct ggcttccggc gctcaccgga gtggtggtcg caagcccggc
      241 ggagagcgac aaatttctct gggatttccg ccggacgatc gccgaactca tcgcccgcga
      301 tcattatggc gaaatctcca agattgcacg cgagcgcggt ctgatcgact acggcgaggc
      361 tctggaagat caccgtccgg ggttcggcga cgacatggaa atgcgccagt tcgccgcgat
      421 cccgaccggc gcgatgtgga cctacggcaa ggaagccggg ccacagccga cctatgtcgc
      481 ggatctcctc ggcgcggcgt cggtggcgca catctatggg cggccacttg tggcggcgga
      541 atcccttact tcggcggggc agccctgggc cttcgcgccg cgcgagttga agccggtcgt
      601 ggacgcggag tttgcgctcg gcgtaaaccg ggtggtgata cacacttccg tgcatcagcc
      661 catcgacaag ccgcccggcc tctcgctctt tggttacggt cagttcttca accggcttga
      721 aagctgggcg caggaagccc ggccatggat aacctatata tcgcgttgct catatctgct
      781 tcagcagggc cgctatgttg cggatgtcgc ttatttctac ggccaggaag cgccgatcac
      841 aggggagttt ggcaccaacc cggtggcgga cgtgccgcag ggatacgctt tcgattttgc
      901 aaattcggac gcactcctga ataagttttc ggttgacaac ggcgatctgg taacgccgtc
      961 gggcatgcgt tatcgcgtgc tgtatctggg cggcagcagc cggttgatga ctctttccgt
     1021 tttgcggcgc attcgtgatc tcgtcgagca aggcgccact cttgtaggga agcgcccgca
     1081 atcatccccg agtctgaagg atgatccggc ggccttccaa aagacggcgg acgaattgtt
     1141 cggtggcgcc gatgagcaca gctacggcaa aggccgcgtg ttcgtttcag gctcgcttcc
     1201 tgaagcactg gcggcgctga atttgccgcc ggattttgaa tacacaaagc cgcaagccga
     1261 tacggaactc cttgctcttc atcgccggct tacggacggc gaactctact tcgtctcaaa
     1321 ccgtcatgac cgtgtcgaga acgtcacagc gacgttccgt gtgacgggct acagtcccga
     1381 aatctggaat gcggtgaccg gcgaaatcgc caaagcgaat ttcaccgccg ccgacggccg
     1441 caccaaagtc tcgctgtcat tgccggcata cggctccgcc ttcgtcgtct ttcgcgacaa
     1501 gaccgaagcc gcggcaaaga tcgcgcccgc atcggcggtt gccacgctcg caaccttgaa
     1561 agggccctgg aagatctcgt tcctgccggg ccgcggcgcg ccggcggcga tcacgcagcg
     1621 cgtccttgaa tcctggagcg acagcaacat ccccggcgtg aagtacttct cgggaacggc
     1681 cacctacact aatacctttg ttctccccaa agcggtgctg aagaacggaa cgcgactggt
     1741 gctcgacctc ggcgaggttc gcgagcttgc ggaagtttcg ctcaacggca aagcagtcgg
     1801 caccgtctgg acggccccgt tccagctcga catcaccaag gcggtgaagc cgggcaagaa
     1861 tgcactgaag atcaaggtcg tcaatttgtg ggtcaaccgc ctgatcggcg acgcgcaacc
     1921 ggaaaacaag aagaagtaca cctttacgac aattccaacc tacaagccgg atgcgccgct
     1981 gagggaatcg ggtttgctgg gccctgtcgt ggtccggcgc gtaaattaac tggagtcaga
     2041 aaatgcaaag ttcagtaggg aaacgtcttc tgccgctatt gctgtgctca gtagtgtttg
     2101 tcggctcgtg tcttgccgag ccgccggcca agtcggatgc gctgctcagc gggttccgca
     2161 atccgcccgc aagcgcgagg ccgggcgtgg gctggccgtg gatgaacggc aatgtcacca
     2221 tcgagggcat cgacaaagac ctttcctgga tgcatcgcgt cggcatcacc agcgtcggca
     2281 tcggcagcgc tgcgatcgat acgccgaccg tcgtgaaaaa gcgtctggag tatctgacgc
     2341 cggaatggaa agaggcgttc cgttatgccg tcggcgaagc ggacaaatac ggaatccagg
     2401 tgggcatcgg cgccgcggcc ggctggagta tgacgggcgg tccgtgggta acggtgcaac
     2461 agtcgatgaa gaagctggtg tggagcgcca cctctgtcga aggcggcaag ccgttccacg
     2521 gtgttctgcc tcagcccccg gatgtgatcg gcccgatcca gaacgccccc tggttcggcg
     2581 atcatgcgcc gacgcagcag accgccaacc tgcatttcta ccgggatgcg atcgtggtcg
     2641 cctatcgcga caccgtcacg gcccccgttg ttgcgtctgc gaccgcaagc accggcaagc
     2701 tcaacgcgaa tatcctggac gacggcgacc tgtccaaggg cgtgatactg gagcccgcca
     2761 cggaagacgg tgatgtgtgg gtccaggtga aatacaagaa gcccgctacg atccagggtg
     2821 tcacgctcgg tctgatcgtg ccgaaagaac tcggctacca tgtcgtcgtc gaagccagca
     2881 ataacggcgg cgattggcgc cacgtggctg acttcccgca ggttgcccag cttcaacgga
     2941 tgcagatggg cgagcagacg atttcgtttg cgcccgtgac cgcccgctat ttccgtctgg
     3001 tgctcaaggc gacgccgccc atcccgacca gctaccgccc gaagaaggga gatgcggcgc
     3061 cggggatgat cccggccgag gccgaggtca cgccgcatcg cgcctatgac gtgcgcgagg
     3121 tcgctttccg tgcaggcgcc acggtcaacg agttcgagaa gaaggcgatg ttcgccgcgc
     3181 cgccgcgcga cttctacaca atcaatagcg taccggacac cgcgccgggt acggccatcg
     3241 atccgtcatc ggttgtcgtg ctaagcgaca agatgaaacc ggacggcacg ctcgattgga
     3301 cgcctccacc ggggaagtgg gtcgtgatgc gcttgggcta ttcgcccgtc ggcaaggaaa
     3361 atcacccggc gccgccggaa gccaccggac ttgaggtcga caagctgaat ggagcgcatg
     3421 tcaggcacta tctcgaacgt tacatcgact tctacaaaac tatcaccggc ccggagttga
     3481 tcggcaagag gggcgttaca tccatcgggc tgggcagttc cgagatcagc gagcagaact
     3541 ggaccgaaaa cataatatca gaattcaagc ggctgcgcgg ttacgatccg tcgccatggc
     3601 tgcctgcttt gaccggcctc gtggtgaaag cccctgcgga cagcgacaaa ttcctctggg
     3661 attggcgcca aacggtggaa gatctgttca aagagaacca ctacggcatg ttctgccagg
     3721 tcatgcacga acacgacctc aagtgcggcg gtcaagcgct ggaagatcat cgtccgacct
     3781 tcggcgacga catggatatg cgccaatact tcgattccgt cggcgcagcc atgtggacct
     3841 atgacagcaa gaagttcccg gccattctgg cttacgaggc ggacatccaa ggggcggctt
     3901 ccgtagcgca catctacggc gccaatctcg tcaacctcga gtccttgact tccgaccgcc
     3961 agccctggtg gtgggcgccg cgcgacacga agaaattcat cgatctggag ttcgcactct
     4021 gcggcaaccg cttgggtatc cacacctccg tacatcagcc ggtggacaag ccgccggggc
     4081 tgtcgcttgg gccgttcggg cagttcttca accgccacga aacctgggcg gaaatggcgc
     4141 ggccgtggct ggactacatc gcgcgcgctt cgtggatgct gcagcaaggt cagtacgtcg
     4201 gcgatatcgt ctacttctac ggccaggagg cgccgatcgt cagcctctgg ggcaacaagc
     4261 gcgtggaagg cgtaccgcaa ggctatgcct tcgacttcgc caacacggat gtgctgctca
     4321 accggctgtc cgtggagaac ggcgcgctgg tgaccaaaac cgggatgcgt taccgcgtcc
     4381 tctatctcgg tggatcgagc gataagatga cgctcacggc gctcaagaaa gtgcgcaaac
     4441 tggtggaaca gggcgcggtc atcgtgggca agcgtccagt ggacacgccg agcctcgtcg
     4501 acgatccggc gcaatggcaa gccgcggcgg acgcggtatt cggcaagccc ggcgaaacgt
     4561 cggtgcgcgc gctcgggcag ggcaaggtga tcgcgtccgg ctcgctggag gaggcgttcg
     4621 cggaactgaa ggttgccccc gacttctcgt acaccggcac ggatgcacaa ctgctctaca
     4681 cccaccggca cctcaaggac ggcgacgtct atttcgtcag caatcgcgag aacaagccgg
     4741 tcacggcctc tgcgagcttc cgcgtggcgg gcaggacgcc gcaattgtgg gatgccatca
     4801 ccggcaagat ctcgccggtc ggctaccgca tcgaagacgg ccgcacgatt gtcaacctcg
     4861 cgctgccggc
//
LOCUS       DASWPU010000391         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_15622,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000391 DASWPU010000000
VERSION     DASWPU010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15622"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VGC27_08425"
     CDS             <1..273
                     /locus_tag="VGC27_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282634.1"
                     /translation="LRTIGKSQTAGNAMSTLMKFRWVIIFVVAIALCVAAANIIEHPS
                     SSPHELLMWGLRGAGYAIAYLCLTFAILAPLIWLLRKRAKDMNKKG"
     gene            367..1338
                     /locus_tag="VGC27_08430"
     CDS             367..1338
                     /locus_tag="VGC27_08430"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY0282635.1"
                     /translation="MSLASAAPPILKTSHLLGIEGISVGEIIGLLDLADEYVALGRAK
                     EKKSPLLKGRTVINLFFEPSTRTQSSFEIAGKRLGADVMNMSVRTSSVTKGETLIDTA
                     TTLNAMRPDILIVRHQDSGAAEMLARKLDCCVVNAGDGSHEHPTQALLDALTIRRHKQ
                     SFEGLTVAICGDVAHSRVARSNIHLLSKMGARVRLIAPRTLLPAAVDRFGVEVFTDMR
                     EGLKGVDIVMMLRLQMERMTGAFIPSTREYFRFWGLDRKKLAFAKPGALVMHPGPMNR
                     GVEIDSAVADDIEVSLIHEQVEMGVAIRMAVLDALSRGLPAEGAGAP"
     gene            1335..>1865
                     /locus_tag="VGC27_08435"
     CDS             1335..>1865
                     /locus_tag="VGC27_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282636.1"
                     /translation="MTNRRTVFCNARLIDPASGLDAKGGLLVENGKIADIGPRLFADA
                     DRNDPETVDCHGLVLAPGLIDMRVFTGEPGSEHRETLESASRAAAAGGVTTIVVMPNT
                     DPVIDEPSLVDFIRRRAAATASVRVAPMAALTRHLAGEVMTEIGLLKEAGAVAFTDGD
                     RTIANTRVLRRALAYAS"
BASE COUNT          322 a          644 c          587 g          312 t
ORIGIN      
        1 ctgcgcacca tcggaaaatc gcaaaccgcg gggaacgcca tgagcactct tatgaagttc
       61 cgctgggtga tcatcttcgt tgtggccatt gccctgtgcg tcgcggcggc gaacatcatc
      121 gagcacccga gttcgtcccc acacgagctt ttgatgtggg ggctgagagg ggccgggtat
      181 gctatcgcct atctgtgcct gacctttgcc atcctcgccc cgctgatctg gctgctccgc
      241 aagcgcgcga aggacatgaa caagaaaggc tgaaccgccc tccgttctcc ttaaccgccg
      301 gaacttgtga gccaaatcag ggaaagcttg ccccctcccc ccggcttgcc tataagccgg
      361 gttttgatga gcttggccag cgccgcaccc ccgatcctga aaaccagcca tttgcttggc
      421 atcgaaggaa tttcggtcgg cgagatcatc ggcctgctcg atctggcgga cgaatatgtg
      481 gcgctcggcc gcgccaagga gaagaagtcg cccctgctca agggccgcac ggtcatcaac
      541 cttttcttcg aaccctccac ccgcacccaa agctcgttcg aaatcgccgg caagcggctg
      601 ggcgccgacg tgatgaacat gtcggtgcgg acctcgtcgg tgaccaaggg cgaaaccctg
      661 atcgacaccg ccaccacgct caacgccatg cgtccggaca tcctcatcgt ccgtcatcag
      721 gattccggcg cggccgagat gctggcccgc aagctggact gttgcgtggt caacgccggc
      781 gacggcagcc acgaacaccc cacccaggcc ttgctcgacg ccctcaccat ccgccgccac
      841 aagcagagct tcgagggcct gacggtggcg atttgcggcg acgtcgccca cagccgcgtg
      901 gcgcgctcca acatccatct gctgtcgaag atgggcgcgc gggtccgcct gatcgcgccg
      961 cgcactttgc tgcccgccgc cgtggaccgc ttcggcgtcg aggtcttcac cgacatgcgc
     1021 gaaggcctga aaggcgtgga catcgtgatg atgctgcgcc tccagatgga gcgcatgacc
     1081 ggcgccttca ttccctccac ccgcgaatat ttccgcttct ggggcctcga ccgcaaaaag
     1141 ctcgccttcg ccaagcccgg cgcgctggtg atgcatcccg gaccgatgaa ccgcggggtg
     1201 gagatcgact ccgccgtcgc cgacgatatc gaggtcagcc tgatccacga acaggtggag
     1261 atgggcgtcg ccatccgcat ggcggtgctg gacgcgctgt cgcgcgggct gccggccgag
     1321 ggagctggcg cgccatgacc aaccggcgaa ccgtcttctg caatgcgcgg ctgatcgatc
     1381 ccgccagcgg gctcgacgcc aagggcgggc tcttggttga aaacggcaag atcgccgaca
     1441 tcggtccgcg cctgttcgcc gatgccgacc gcaatgaccc ggagaccgtc gactgtcacg
     1501 ggctggtgct ggcgcccggg ctgatcgaca tgcgggtgtt caccggcgag ccgggcagcg
     1561 agcatcgcga gacgctggaa tccgccagcc gcgcggccgc ggcgggcggc gtcaccacca
     1621 tcgtcgtcat gccgaacacc gatccggtga tcgacgagcc gtcgctggtc gatttcatcc
     1681 gccggcgcgc cgccgcgacg gcttcggtgc gggtcgcgcc gatggccgcg ctgacccgcc
     1741 atctggcggg cgaagtgatg accgaaatcg gcctgctcaa ggaagcgggc gcggtggcgt
     1801 tcaccgacgg cgaccgcacc atcgccaata cgcgcgtgct gcgccgggcg ctggcctacg
     1861 cctcc
//
LOCUS       DASWPU010000392         3576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_27554,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000392 DASWPU010000000
VERSION     DASWPU010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27554"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..680)
                     /locus_tag="VGC27_08440"
     CDS             complement(6..680)
                     /locus_tag="VGC27_08440"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEY0282637.1"
                     /translation="MTSTSWLSKVLRWMFAVFSILAALFAVALCVLMAVDPKLPPGTH
                     FGPLAANFMGQPGTIAMQDSTVTLTVLHGGIKASVESAGDLVELLKHYGLPVLILNAV
                     FFAVLFDLLRRLFRNVGRGESFTRQSVQLVQIIGVSLLAFSLVSAFAESWFFHAIYSY
                     LSQHSVIVVSGTPLHLPQPQGMKIPSGHGFPFGSPLFFSGLLVLALSEVFRQGLALKH
                     ENDLTV"
     gene            complement(730..1236)
                     /locus_tag="VGC27_08445"
     CDS             complement(730..1236)
                     /locus_tag="VGC27_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282638.1"
                     /translation="MIIRAILRLDSLRDQSKGNHICVLARRALLHPQRHAQRDRGQRQ
                     AARPFLVQVNSYIFLLLFIDKSVLSINVGASFPDKDRRTSVNFVSRQAAIGAVVAAAL
                     MLGANASPDTRPGMPSHSASTIGAQLTQSYQGTGAILAEMFQVCRDKILNETLDTFGE
                     IAARLKQL"
     gene            1252..1716
                     /locus_tag="VGC27_08450"
     CDS             1252..1716
                     /locus_tag="VGC27_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY0282639.1"
                     /translation="MSRVLAVTAAALFAVPVLAHAGERQDAKVLMATEPADLKLQELV
                     GFSDAVVTGDTIYFSGLVAEPNPGETDLRPAYERAFVRLGQILTRAGASWDDVVEITS
                     FHTDMRTQMGPMIEVKNRHLREPHPAWTAIGISHLGAPRAVTEIRMIAKLPK"
     gene            complement(1786..3156)
                     /gene="tig"
                     /locus_tag="VGC27_08455"
     CDS             complement(1786..3156)
                     /gene="tig"
                     /locus_tag="VGC27_08455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY0282640.1"
                     /translation="MQITETVSEGLRREYKVVVGAADLEERLTGKLNEIKPRLSLKGF
                     RPGKAPVSFLKKTYGKSMMGEIVEAAVNEGSQKAVTDNALKVAYPPRVEPVGDVQQVI
                     DGKADLEFRVLVDLMPDFELADVSKIEVERLVADISDEDLEQAVTRLAEQSRSYSPRA
                     EGEAAEKGDAVTMDFVGSVDGEEFPGGKAENFNLVLGSEQLIAGFEDQLIGAKAGEAR
                     DVKVTFPADYPEAKLAGKEAVFAVTVKEVKSPDPLVIDDELAKKLGVDSLGTLKERLR
                     DQMKRDYAAASRLHLKRRILDALDERHSFSLPPTMVEGEFDGIWRQVESELKRENKTF
                     EDEGKSEEELRKEYREIAERRVRLGLVLARLGEQNGLTVANEEIQRAIAARARQFPGQ
                     EQQVFDYYARNAQAQAEIRAPLFEDKVVDFIAELATVTDRKVDRETLFLDPDEAAEKL
                     KGEKSE"
     gene            complement(3346..3430)
                     /locus_tag="VGC27_08460"
     tRNA            complement(3346..3430)
                     /locus_tag="VGC27_08460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3394..3396),aa:Leu,seq:tag)
BASE COUNT          648 a         1152 c         1088 g          688 t
ORIGIN      
        1 gcatttcaga ccgtcaggtc gttttcatgc ttcagcgcca gtccctgacg gaagacctcc
       61 gacagcgcca gcaccagaag ccccgagaag aacaaggggc tgccgaacgg gaatccgtga
      121 ccgctgggga tcttcatgcc ctggggctgc ggcaggtgca gcggcgttcc ggaaaccacg
      181 atcaccgaat gctgcgacag ataggagtat atcgcatgga aaaaccagct ttcggcgaac
      241 gccgacacca gcgagaaggc cagcagcgaa acaccgatga tctgcacaag ctggacgctt
      301 tgccgggtga agctttcgcc gcgcccgacg ttgcggaaca gccggcgcag aagatcgaac
      361 agcacggcga agaaaaccgc gttgagaatg aggaccggca agccgtaatg cttgagcagt
      421 tcgaccaggt cgccggcgct ctccacgctc gctttgatgc cgccgtgaag aaccgtgagg
      481 gtgacggtgg agtcctgcat ggcgatcgtg ccgggttgcc ccatgaaatt cgcagccagc
      541 ggcccgaaat gcgtgccggg cggcagcttc ggatcgaccg ccatgagcac acagagcgcg
      601 acagcgaaga gtgccgccaa aatagagaaa acggcgaaca tccaacgcag gaccttggag
      661 agccaactgg tgctggtcat ggcgatcact ttccggtttt cagcgctcgc cccgcgagcc
      721 gttcctttat cacagttgct tcaggcgcgc ggcaatctcg ccgaaagtat cgagcgtctc
      781 gttgaggatt ttgtcccggc aaacctggaa catctcggcg aggatggctc cagtcccctg
      841 ataggactgc gtcagttgcg ccccgatggt cgaagcgctg tgagacggca tgccgggccg
      901 ggtgtccgga ctggcgtttg ccccgagcat gagggccgca gcgacaacgg cgccgatggc
      961 ggcttggcgg ctgacaaagt tcacggaggt cctcctgtcc ttatcgggaa acgatgcacc
     1021 aacattgata ctcaatacag atttatcgat aaacagcaat aggaaaatat agctatttac
     1081 ctgcacgaga aagggacggg cggcttgccg ttgtcctctg tctctctgcg cgtgtctttg
     1141 ggggtgcagc aacgcccgcc gcgccaaaac gcagatgtga ttgccttttg attgatctcg
     1201 cagcgagtcg aggcgcaaga ttgcacggat gatcaatcgg gggtgccgtt catgtctcgc
     1261 gttctcgccg tcaccgcagc ggctttgttt gccgttcccg tcttggccca tgcaggcgag
     1321 cgccaggacg ccaaggtgct gatggccacc gaaccggccg atctcaagct tcaggaactc
     1381 gtgggttttt ccgacgccgt ggtcaccggc gatacgatct atttttcggg ccttgtcgcc
     1441 gagccgaacc cgggcgaaac cgatcttcgc ccggcctatg agcgcgcgtt cgtccgcctc
     1501 ggccagatac tcacccgcgc cggcgcgagc tgggacgatg tcgtcgagat caccagcttc
     1561 cacaccgaca tgcggaccca gatggggccg atgatcgaag tgaagaacag gcacctccgc
     1621 gagccgcatc cggcctggac cgctatcggc atctcgcatt tgggcgcccc gcgcgccgtg
     1681 accgagatcc gcatgatcgc caaactcccg aaatagcccg cgcagagctg aaacaaaaaa
     1741 gggcgccggt gagggcgccc tttccttttg gcgatcggca ggcggctatt cgctcttctc
     1801 gcccttcagc ttctccgccg cctcgtcggg atcgaggaac agcgtctcgc ggtcgacctt
     1861 gcggtcggtc acggtcgcga gttcggcgat gaagtccacc accttgtcct cgaacagcgg
     1921 cgcgcggatt tcggcctgcg cctgggcgtt gcgggcgtaa tagtcgaaca cctgctgctc
     1981 ctggccaggg aactggcggg cgcgggcggc gatggcgcgc tggatttcct cgttggcgac
     2041 ggtcaggccg ttctgctcgc ccagccgcgc cagcaccaga ccgagccgca cccggcgttc
     2101 ggcgatctcg cgatactcct tgcgaagctc ttcctcgctt ttgccttcgt cctcgaaggt
     2161 cttgttctcg cgcttcagtt cggactcgac ctggcgccag atgccatcga actcgccttc
     2221 caccatggtc ggcggcagcg agaaggaatg ccgttcgtcc agcgcgtcga ggatgcggcg
     2281 cttgagatgc aggcggctgg cggcggcata atcgcgcttc atctggtcgc gcagacgttc
     2341 cttcagcgtg ccgagggaat ccacgcccag cttcttggcg agttcgtcgt cgatcaccag
     2401 cgggtcgggc gactttacct ctttcaccgt gaccgcgaac acggcttcct tgccggcaag
     2461 cttggcttcg ggataatccg ccgggaaggt cactttgacg tcgcgcgcct cgcccgcctt
     2521 ggcgccgatg agctgatcct cgaaaccggc aataagctgc tccgaaccga gcacgaggtt
     2581 gaaattttcc gccttgccgc cgggaaattc ttcgccgtcc acggagccga cgaaatccat
     2641 cgtcaccgcg tcgccctttt cggcggcttc gccttcggcg cgcggggaat agctgcgcga
     2701 ctgttcggcc aggcgcgtga ccgcttgctc cagatcctcg tcgctgatat cggccaccag
     2761 gcgctcgacc tcgatcttag agacgtcggc cagctcgaaa tcgggcatca ggtcgaccag
     2821 gacgcggaat tcgagatcgg ccttgccgtc gatcacctgc tgcacgtcgc cgaccggctc
     2881 gacccgcggc gggtaggcca ccttgagcgc gttgtcggtg accgccttct ggctgccttc
     2941 gttgaccgcc gcttcgacga tctcgcccat catcgacttg ccgtaggtct tcttgaggaa
     3001 cgagaccgga gccttgcccg gcctgaagcc cttgaggctg aggcgcggtt tgatttcgtt
     3061 gagcttgccg gtcaggcgct cttccagatc ggcggcgccg accacgactt tgtattcgcg
     3121 gcgcaggcct tcggaaacgg tctcggtaat ctgcatgtca gttccctacc taaatctcga
     3181 ccagcccgtc ttcgccgtgg cagccttcgc tttcgctggc ttgtccagtc gaagcccctg
     3241 cgcacgggag cgaagaccgg cttgccgcgc gtcgctcgtg ccaaccaccc cggcttcgcc
     3301 aaccggcacc gccgggcacg cttctctcgc accgcgagcg aaacgtggtg cgggcggagg
     3361 gacttgaacc cccacgactt tcatcactgg aacctaaatc cagcgcgtct accagttccg
     3421 ccacgcccgc tctgtcagcc gctgtcgcgg gcgcgggctt ataacatggg gtagcgcctg
     3481 cgcagcaaaa aaatggcccg gttttgccca atccgatttc ccgcggcggc tggacatctc
     3541 acctcaaaaa tggtccgcat cggcttcatg gcaagg
//
LOCUS       DASWPU010000393         4731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_9042, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000393 DASWPU010000000
VERSION     DASWPU010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9042"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VGC27_08465"
     CDS             complement(<1..356)
                     /locus_tag="VGC27_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY0282641.1"
                     /translation="MQLHLSSWPEIEAYLTASKAILIPIGSTEQHGPNGLLGTDALCA
                     EVIARRAGDEAGILVGPTFNVGQAQHHMAFPGTITLRPTTMIAAMFDWAQSLARHGFE
                     RLYWLNGHGGNTATINA"
     gene            complement(356..907)
                     /locus_tag="VGC27_08470"
     CDS             complement(356..907)
                     /locus_tag="VGC27_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282642.1"
                     /translation="MAAKSYRPAMSDLAVVTKTGKTWKQWFGMLDKAGAAKLDHAAIA
                     KLLSAKHSVGPWWRQMVAVEYERARGLRTKHETATGFSVSMSKTMAGDVAALYAATDD
                     AGRRKKWFPAGALKVSSQTPGKYFRGRWKGAVRLEINFYAKTGGKAQITVQVGKLAKK
                     SDVESERAAWKKALGKLETILST"
     gene            1011..2951
                     /gene="acs"
                     /locus_tag="VGC27_08475"
     CDS             1011..2951
                     /gene="acs"
                     /locus_tag="VGC27_08475"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY0282643.1"
                     /translation="MTDTLLPVPAEWKHRAFIDAAKYDEMYTRSLADPEGFWREQAAR
                     LDWIKPFTKVKNVSWDPDDFHVKWFEDGSLNVAANCIDRHLTAHGDRIAIIWEGDDPK
                     DSRIITYQELHDEVCRFANVLRAHGVKKGDRVTIYLPMIPEAAYAMLACARIGAVHSV
                     VFAGFSPDSLANRILDCASTIVITADEGRRGGKPVPLKTNTDLALLQCPEVRHVIVVR
                     RTGKHELMHPGRDVWYHEAAALVTAECAPVEMNAEDPLFILYTSGSTGKPKGVLHTTG
                     GYLLYAAFTHQIVFDYHEGDVFWCTADVGWVTGHSYVVYGPLANGATTLMFEGVPNYP
                     TASRFWEVVDKHKVNIFYTAPTAIRALMRDGEAPVKKTSRASLRLLGSVGEPINPEAW
                     LWYHRVVGEGRCPIVDTWWQTETGGILISPLPGATDLKPGSACLPLFGIRPQIVDAEG
                     ATLDGAASGNLVLLDSWPGQMRTVYGDHQRFVNTYFRTYRGKYFTGDGCRRDEDGYYW
                     ITGRVDDVINVSGHRLGTAEVESALVENLKVAEAAVVGCPDDLKGQGIYAFVTLKAGE
                     APTVKLNEELKKFVSEHLSPIARPDVIQFAPGLPKTRSGKIMRRILRKIAEGDISNLG
                     DTSTLADPAVVTDLVDHAKRTI"
     gene            3132..>4731
                     /locus_tag="VGC27_08480"
     CDS             3132..>4731
                     /locus_tag="VGC27_08480"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0282644.1"
                     /translation="MSVQNRIVSLAVLAAVAVPASAQTPDSQVSSIETVVVTAQKRVQ
                     NPIDVPMALTAYSGTFMESVGIQEFDKLSLFVPGFVVQNQSPNNPGFGLRGLNLDSGE
                     AAQEPRVSVFEDDVSISTTRATYIELFDIERIEVVKGPQTTLFGRAALMGGVNVIQNK
                     AKLDGFHAAASIEGGDYNYYMGEAMVNVPLTDTLAMRIAGRYKSRNGYVENLGIGNDY
                     NSVNTAAGRLSFAWTPSEAFSSDLIFNYERDAPSGTSFKSGTFAPADAATGAILGTID
                     HNTGATLTNSVSGFEDDKQVGLNRKVWDVKSLNTYKFSPELTLSTVTAFRRFDSLEIF
                     DPDGFVQPLLQAAEDERGDQFSHEMRLNFENGGRFSGFVGANYFYSNVSQRVPFQFDE
                     RAALALVGGQGALLQNMPTAFFSSPAYTAGYAPTILRGVARATVYNTVYNMLVGTYGE
                     AVAAAQANAQADVYVAANSATLNYLAAGMKSNHWEESTNYGKTKSVDLYGDFTYRVTN
                     ALELNAGLRFTHDDKTTSYASTIGD"
BASE COUNT          924 a         1569 c         1393 g          845 t
ORIGIN      
        1 gcgttgatcg tggcggtgtt gccgccatgg ccgttgagcc aataaagccg ctcgaagccg
       61 tgacgcgcca gcgattgcgc ccaatcgaac atcgccgcga tcatggtggt gggcctgagc
      121 gtgatggtgc cggggaacgc catgtgatgc tgcgcctgcc ccacgttgaa ggtcgggccg
      181 accaggatgc ccgcttcgtc gcccgcgcgc cgcgcgatga cctccgcgca cagcgcgtcg
      241 gtgccgagca agccattcgg gccatgctgc tcggtcgagc cgatgggaat gaggatggct
      301 ttggatgccg ttaggtaagc ttcgatctcg ggccaggagg agaggtggag ctgcatcacg
      361 tactcaagat cgtttcaagc ttgccaagcg ctttcttcca tgccgcccgt tccgactcaa
      421 catcggactt cttcgccagc ttgccgacct ggacagtgat ctgcgccttg ccgccggttt
      481 tggcgtagaa attgatctcc agccgcaccg cgcctttcca gcgtccacgg aaatatttgc
      541 caggggtttg agaggaaacc ttcagcgcac ctgcgggaaa ccatttcttg cgccgcccag
      601 cgtcgtcggt cgcggcatag agcgcggcga catcgccagc catggtcttc gacatgctga
      661 ccgagaaccc cgtcgcagtc tcatgcttgg tgcgcaggcc gcgggcgcgc tcgtattcca
      721 cggctaccat ctgccgccac caggggccta cgctatgctt ggccgacagc agctttgcaa
      781 tggcggcgtg gtccagtttg gcggcacccg ccttatcgag catgccgaac cattgcttcc
      841 aggtcttgcc ggtcttggta acgacggcga gatcggacat cgccgggcga taggactttg
      901 cagccatgcc aatatccttc cgtttcaaca gcctgcggac accttgaagg ttcggccgcc
      961 ctcggtcaca atagaattat aataacgcca aggaagccca gggaaaagcc atgaccgata
     1021 cgctgttacc ggtacccgcc gaatggaagc atcgcgcctt catcgacgcc gccaaatacg
     1081 acgagatgta cacccgctcg ctcgcggacc cggaaggctt ctggcgagag caggcggcgc
     1141 ggctcgattg gatcaagccc ttcaccaagg tgaagaacgt ctcctgggac ccggacgatt
     1201 tccatgtcaa atggttcgag gacggatcgc tgaacgtcgc cgccaactgc atcgaccggc
     1261 acctgacggc ccatggcgac cgcatcgcga tcatctggga aggcgacgac ccgaaggatt
     1321 ccagaatcat cacctaccaa gaactgcacg acgaagtgtg ccgcttcgcc aacgtgctgc
     1381 gcgcccatgg cgtcaagaaa ggcgaccgcg tcaccatcta tctgcccatg atcccggaag
     1441 cggcctacgc catgctggcc tgcgcgcgca tcggcgcggt gcattcggtg gtgttcgccg
     1501 gtttctcgcc cgacagcctg gcgaaccgca tcctcgattg cgcctcgacc atcgtcatca
     1561 ccgccgacga aggaaggcgc ggcggcaagc cggtgccgct gaagaccaac accgatctgg
     1621 cgctgctgca atgcccggag gtgcgccacg tcatcgtcgt gcgccgcacc ggcaagcacg
     1681 aactcatgca tcccggacgc gacgtctggt atcacgaagc ggcggcgctg gtgacggccg
     1741 agtgcgcccc ggtggagatg aacgcggaag acccgctgtt catcctctat acctccggct
     1801 cgacgggaaa gcccaagggc gtgctgcaca cgacgggcgg ctacctcctc tacgccgcct
     1861 tcacccacca gatcgtgttc gattaccacg agggcgatgt gttctggtgc acggccgacg
     1921 tgggttgggt gacgggccac agttacgtcg tctacggacc gctggcgaac ggcgccacca
     1981 cgctgatgtt cgaaggcgtg ccgaactacc ccacagcttc gcgcttctgg gaagtggtcg
     2041 acaagcacaa ggtcaacatc ttctacaccg cgccgaccgc gatccgcgct ctgatgcgcg
     2101 acggcgaagc gccggtgaag aaaacctcgc gcgcttcgct gcgcctgctc ggcagcgtcg
     2161 gcgagccgat caatccggaa gcctggctgt ggtatcaccg cgtcgtcggc gagggccgct
     2221 gccccatcgt ggatacttgg tggcagaccg agaccggcgg cattctcatc tcgccgctgc
     2281 cgggcgccac cgacctcaag cctggttctg cgtgcctgcc cttgttcggc atccggccgc
     2341 agatcgtcga tgccgaaggc gcgacgctcg acggcgccgc cagcggcaac ctggtgctgc
     2401 tggattcgtg gccgggccag atgcgcaccg tctatggcga ccatcagcgt ttcgtgaaca
     2461 cctatttccg cacctatcgc ggcaaatatt tcaccggcga cggctgccgc cgcgacgagg
     2521 acggctatta ctggatcacc ggccgcgtgg acgacgtgat caacgtctcc ggccaccggc
     2581 tgggaaccgc cgaagtggaa tccgccctgg tggagaacct caaagtcgcc gaggccgcgg
     2641 tggtcggctg tccggacgat ttgaaaggcc aaggcatcta tgccttcgtc actctcaagg
     2701 cgggcgaagc gccgaccgtc aagctcaacg aggaattgaa gaagttcgtg tcggaacacc
     2761 tctctcccat cgcccgaccg gacgtgatcc agttcgcgcc cggcctaccg aaaacgcgct
     2821 cgggcaagat catgcggcgc atcctgcgca agatcgcgga aggcgacatc tccaatctcg
     2881 gcgacacatc cacgctggcc gatccggccg tggtgaccga tctggtcgat cacgccaagc
     2941 gtacgattta gcccggtaag ccgcatccgg cctgccgctg gaggaacaga cgcggcaatt
     3001 ccaggcagat aagtcacggt cgtaaaataa tctttgcgct gcggcatcaa tagccgcccc
     3061 gccttcatta cagtgacaca taggtcacct agcgatgatg ccatcgggta atcagaacta
     3121 ggggtgtcgc catgtccgtt caaaaccgga ttgtttcgct tgccgtgctg gccgcggttg
     3181 cagttcctgc gtccgcgcaa acgcccgaca gccaagtatc gagcatcgaa accgtggtcg
     3241 tcaccgcgca gaagcgcgtg cagaatccga tcgacgtgcc tatggcgctg accgcctact
     3301 ccggcacctt catggaatcc gtcggcatcc aggaattcga caagctttcg ctcttcgtcc
     3361 ccggcttcgt ggtgcagaac cagtcgccca acaatcccgg tttcggcctg cgcggcctca
     3421 acctggattc cggcgaggcc gcccaggaac cgcgcgtttc ggtgttcgag gacgacgttt
     3481 ccatctccac cacccgcgcc acctatatcg agttgttcga catcgagcgc atcgaagtcg
     3541 tcaaggggcc gcagaccacc ttgttcggcc gcgcggccct gatgggcggc gtcaacgtca
     3601 ttcagaacaa ggccaagctc gacggtttcc acgccgcggc cagcatcgaa ggcggcgatt
     3661 acaactacta catgggcgaa gccatggtga acgttccgct caccgacacg ctcgcgatgc
     3721 gcatcgccgg ccgctacaag agccgcaacg gctatgtgga aaatctcggg atcggcaacg
     3781 attataattc ggtcaatacg gccgcggggc ggctgtcgtt tgcctggact ccgtccgagg
     3841 ctttctcgag cgacctcatc ttcaactatg aacgggacgc cccgagcggc acgtcgttca
     3901 aatccggcac cttcgcgccg gccgacgcgg ctaccggcgc catcctcggc accatcgatc
     3961 acaacacggg cgccacgctc accaacagcg tcagcggctt cgaagacgac aaacaagtcg
     4021 gcctcaaccg caaggtttgg gacgtcaagt cgctgaacac ttacaaattc agtccggaac
     4081 tcacgctttc gacggtgacg gccttccggc gtttcgactc gctggaaatc ttcgacccgg
     4141 atggtttcgt gcagccgctg cttcaggccg ccgaggacga gcgcggcgat caattcagcc
     4201 acgaaatgcg gctcaacttc gagaacggag gaaggttcag cggattcgtc ggcgccaact
     4261 atttctacag caacgtctcg cagcgggtgc cgttccaatt cgacgagcgc gcggcgctgg
     4321 cgttggtcgg gggccagggc gcactgctgc agaacatgcc caccgcgttc ttcagttcgc
     4381 ccgcctatac ggccggctac gcccccacga tcctgagggg cgtcgcccgc gcgaccgttt
     4441 acaacaccgt ctacaacatg ctggtcggga cttatggcga agccgtcgcc gccgcccagg
     4501 ccaacgccca ggccgacgtc tatgtggccg cgaacagcgc cacgctcaac tatctggccg
     4561 ccggcatgaa atccaaccac tgggaagaat cgaccaacta cggcaagacc aaatcggtcg
     4621 atctgtacgg cgacttcacc taccgcgtca ccaacgcgct cgaactcaac gccggcctcc
     4681 gcttcaccca tgacgacaaa accacctcct atgccagcac aattggcgat a
//
LOCUS       DASWPU010000394         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_76430,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000394 DASWPU010000000
VERSION     DASWPU010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76430"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VGC27_08485"
     CDS             <1..263
                     /locus_tag="VGC27_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282645.1"
                     /translation="PAKTAFPDGPPGGLGGAQIKSAKNINVVVMADTDIFDNRFWVRL
                     GSLYGKQVASPIADNGAFVMNAVENLMGSGDLISLRTRATDD"
     gene            276..635
                     /locus_tag="VGC27_08490"
     CDS             276..635
                     /locus_tag="VGC27_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282646.1"
                     /translation="MQDLQARAEAQYRQEAQALQQRLTDTQQRLHDLQQGQGGEGDAV
                     VLTPPQQAEIERFKREMIQTRGQLRDVQHNLRKDIDLLGDILAFANIVFVPLLVAGFA
                     LALAGLRRRRRARAIKL"
     gene            643..1719
                     /locus_tag="VGC27_08495"
     CDS             643..1719
                     /locus_tag="VGC27_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282647.1"
                     /translation="MKLKFFSSDPHRRNLAVLAALAFVSVLLAVLALDRRAAEVAPKY
                     PPQATFPGLASKLSEVTRIHIESRKGGAFDVAFVPMKGWVLPGRNHYPASFEEVRKTL
                     VGLAALQTIEPKTARPDWFRYVDLDAPPKGGGVLISLHGDKDRVLAALIVGKSEDIGD
                     ATQSVGLFVRKPGESQSWLVSSPFTPHANQIGWLKKNIVEVDRSRIQETDVRPLSGPA
                     YTVRRDKPSDMDFALTPMPRGRELADSGGPDGVASALVDFSFDDIQPAANLDFESQAW
                     RTVTRTFDGLIVGVDVVKQGPDYWARIFAYAAPGKPDAAKEARDINSSAQGWAYKLPA
                     YKGALFMLPLDSLLKPAGTAKKTK"
     gene            2147..>2569
                     /locus_tag="VGC27_08500"
     CDS             2147..>2569
                     /locus_tag="VGC27_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282648.1"
                     /translation="MPKRLDIHTVLIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPITWQVVEKIIAKERPDAVLPTMGGQTALNCALDL
                     HKHGVLAKYGVEMIGANEKAIEKAEDRLKFKDAMTSIGL"
BASE COUNT          482 a          852 c          791 g          444 t
ORIGIN      
        1 gcccagccaa aacggccttt cccgacgggc ctcccggcgg cctcggcggc gcgcagatca
       61 agagcgcgaa gaatatcaac gtcgtggtca tggccgacac cgacattttc gacaaccgct
      121 tttgggtgcg gctcggcagc ctctacggca agcaggtcgc ctcgcccatc gccgacaacg
      181 gcgctttcgt gatgaacgcg gtcgagaatc tgatgggttc gggcgatctg atttcgctgc
      241 gtacgcgcgc gaccgacgac tgacccttca ccgtggtgca agacctccag gcccgggccg
      301 aggcgcagta ccggcaggaa gcgcaagcct tgcagcagcg cctcaccgac acccaacagc
      361 gtcttcacga cctgcagcag ggccagggcg gcgaaggcga cgctgtcgtg ctcaccccgc
      421 cgcaacaggc ggaaatcgaa cgcttcaagc gcgaaatgat ccagacgcgc gggcagttgc
      481 gcgacgtcca gcataatctg cgcaaggaca tcgacctctt gggcgatatc ctcgccttcg
      541 cgaatatcgt cttcgtgccg cttctggtcg cgggcttcgc gcttgcactg gccggcttgc
      601 gccgccgccg ccgcgcgcgc gccatcaagc tgtgagggcg cgatgaagct gaaattcttc
      661 tcctccgatc cgcaccggcg caatctcgcc gtccttgccg cgctggcgtt cgtcagcgtg
      721 ctcctggccg ttttggcgct cgaccggcgc gcggccgaag tggcgccgaa atatccgccg
      781 caggccacct tccccggatt ggcttccaag ctcagcgagg tcacgcgcat ccatatcgaa
      841 tccagaaagg gcggggcttt cgacgtggcg ttcgtgccga tgaagggctg ggtcctgccc
      901 ggccgcaacc attatccggc ctcgttcgag gaagtgcgca agactctcgt cgggctggcg
      961 gcgttgcaga ccatcgagcc gaagaccgca cgccccgatt ggttccgcta tgtcgatctc
     1021 gacgcgcccc cgaagggcgg cggtgtcctg atctctctcc acggcgacaa ggaccgcgtg
     1081 ctggcggcac tgatcgtcgg caagagcgag gatatcggcg acgccacgca gtcggtgggc
     1141 ctgttcgtgc gcaagcccgg cgagagccaa agctggctgg tcagcagccc cttcaccccg
     1201 cacgcaaacc agatcggctg gctgaagaag aatatcgtcg aggtggaccg cagccgcatt
     1261 caggaaaccg atgtccggcc cctgtcgggg cccgcctaca cggtccgccg tgataagccg
     1321 agcgacatgg atttcgcgct gacccccatg ccgcgcggcc gcgaactggc cgattccggc
     1381 ggccctgacg gcgtggcctc cgccctggtt gatttctcct tcgacgacat tcagcccgcc
     1441 gcgaatctcg atttcgaaag ccaggcttgg cgcaccgtca cccgtacctt cgacggactg
     1501 atcgtcggcg tggacgtcgt caagcaaggc ccggactatt gggccagaat cttcgcctac
     1561 gccgcaccag gcaagcccga cgccgccaag gaagcccgcg acatcaactc cagcgcgcag
     1621 ggctgggcct acaagctgcc agcctataaa ggcgccctgt tcatgctccc cctcgacagc
     1681 ctgctgaagc cggccggaac ggcgaaaaag actaaataga ggaagaatca caccttcttc
     1741 atctagggac ttaaatcggc tctgccctga tgctaaggaa ggccgtgccg ccagcatggt
     1801 acggcctctg cgacaaggtt ccagtcgagt cggggttgtc gtcggcgggt ccggtgtggg
     1861 agataacgct gcgatgaaga cgtcgaagac aggatttgga cggccctccc ggcatccggc
     1921 gagggctttt ttttgagtgc tactacaatg tcgaagtgtg gagttcccga accacaggtg
     1981 tcatggcccg cgtccgcaca ctgtcgctac ggaccgggcc atccagttga cacttgcgga
     2041 tgccttgaag tctttctccg gcgctccatt ctgaaccatc gtgcgtgtca tcacctgggt
     2101 ggcccgcatt cgcgggccat gacaattttg gctttggggt ccagtcgtgc ctaagcgcct
     2161 cgacatccac accgttctga tcatcggcgc cgggcccatc gtcatcggcc aggcctgcga
     2221 gttcgactat tccggcgccc aggcctgcaa ggcactgcgc gaagagggct acaaggtcat
     2281 cctggtcaac agcaacccgg cgaccatcat gaccgacccc gagatggccg atgtgaccta
     2341 catcgagccc atcacctggc aggtggtcga gaagatcatc gccaaggagc gccccgatgc
     2401 ggtgctgccg acgatgggcg ggcagacggc cttgaactgc gcgctcgacc tgcacaagca
     2461 cggcgtgctc gcgaagtacg gcgtcgagat gatcggcgcc aacgagaagg cgatcgagaa
     2521 ggccgaagac cgcctgaagt tcaaggacgc gatgaccagc atcggcctc
//
LOCUS       DASWPU010000395         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_52139,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000395 DASWPU010000000
VERSION     DASWPU010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52139"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3473
                     /locus_tag="VGC27_08505"
     CDS             <1..3473
                     /locus_tag="VGC27_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase/phage tail family protein"
                     /protein_id="HEY0282649.1"
                     /translation="DGNLIDLSAYTTRFYPGDETQLPDPLIEEVEGAAPAYRGLCYLV
                     FEDMALADFGNRIPQLQFEIVRAISSDNPAALENRLHGVALIPGAGEFVYATEAVTQD
                     NGLGTTSPENAHNASGETDLKASLDELQGLAPNLGAVSLVVGWFGDDLRAGSIQIKPG
                     VEAAAKLTYPETWSVAGVARASAHLVSRIDGRPAYGGTPSDQSVVAAIAELKARGLRV
                     LFNPFLFMDVTADNALPDPYSDNGARAGQPAYPWRGRITVSPAAGFAGSVDKTAAAAQ
                     IGAFFNGAWGYRRMILHYATLCAAAGGVDAFLIGSELRALTRARSDASTYPAVAALKT
                     LAAEVRAILPAAKIGYGADWSEYNNHQTGDAPGALRFNLDPLWSDANIDFVGIDNYLP
                     LADWRDGAAHLDYDAQNGPTSIHDPAYLAANIRGGEDFDWYYASDADRASQTRTPITD
                     GLGKPWVWRAKDLWSWWSNPHYDRPSGSESASHTAWVPQSKPIWFTELGCPAVEKGAG
                     QPNVFYDPKSSESFFPYYSTGERDDLIQRRFLEAQLDFWNRPANNPVSDVTHAPMLDL
                     SNLYVWCWDARPFPFFPARADVWGDAANYRTGHWLNGRLGAVQLSDLVAALCAETNFA
                     EFDASGLTGLVTGFAVTDTMSVRDALAPLSGAYFFDGVESEGLIKFVMRGRPAALPLA
                     ESDLALDDGDSSFGFSFERIQETDLPLASRITYLDADADYRQAVAEARRLVALSDRIA
                     TSSLPLVFDQGQAIGIGQRLLQDAWVMRESAKFSLGPSRLALDPTDEVLLEAGGRTRR
                     LRLTGIDDAGARRIEAVATDPSVYETIVGSARSTAATQSLRQPGRALVVFLDLPLLTD
                     NQNPNAPLVAAYADPWPGAVQVLKSPTDSNYALDTSVTRPATLGTTLWDFWSGPLWRW
                     DRVNTLYVKLLSGALASADDASVFAGANALAVQNADGEWEIVQFATAELTAPDEWRLT
                     RLLRGQHGSEGAMRNPVAAGARVVVLDEALAQLSLSQAQARWPFAYLWGPAGKPISDP
                     AFQGATLAFEAAGLIPPAPCHVRFAWSGNDLIFSWKRRDRASAAANLLQAETPLSESG
                     EFYDLEILNGGALARTFSSIPQHSQIYTAAQQAADFPSGLPNPLVVNVYQRSSVLGRG
                     RQKTESLYVR"
BASE COUNT          551 a         1248 c         1165 g          621 t
ORIGIN      
        1 cggacggcaa tctgatcgat ctttcggcct ataccacgcg cttctatccc ggcgacgaaa
       61 cgcagctccc cgatccgctg atcgaggagg tcgaaggggc cgcgcccgcc tatcgcgggc
      121 tttgttacct cgtgttcgag gacatggcgc tggccgattt cggcaaccgc attccgcaat
      181 tgcagttcga gatcgtgcgc gcgatctcgt cggacaatcc tgccgcgctg gaaaaccggc
      241 ttcatggcgt ggcgctgatc cccggcgcgg gcgagttcgt ctatgccacc gaggccgtca
      301 cccaggataa cggcttggga accacgtcgc ccgagaacgc gcacaatgcc tccggcgaaa
      361 ccgatctcaa ggcctcgctc gacgaattgc agggcctggc gcccaatctc ggcgcggtgt
      421 cgctggtggt gggctggttc ggcgacgatc tgcgcgccgg ctccatccag atcaagccgg
      481 gcgtggaggc tgcggccaag ctcacctatc ccgagacttg gagcgtcgcc ggcgtggcgc
      541 gcgccagcgc gcatctggtc agccggatcg acgggcggcc cgcttacggc ggcacgccgt
      601 cggatcaaag cgtcgttgcc gccatcgccg agctgaaagc gcgcgggctg cgcgtgctgt
      661 tcaatccgtt cctgttcatg gacgtgactg cggacaacgc gctgcccgat ccctattcgg
      721 acaatggtgc gcgtgcgggt cagccggcct atccttggcg cggccgcatc acggtgagcc
      781 cggcggcggg cttcgcaggc agcgtggata agaccgccgc cgccgcgcag atcggcgcct
      841 tcttcaacgg cgcctggggc taccggcgca tgatcctgca ttacgccacg ctgtgcgcgg
      901 cggcgggcgg cgtcgatgcg ttcctgatcg gctccgaact gcgcgccctc acgcgcgcgc
      961 gcagcgacgc aagcacctat cccgccgtcg cggcgctgaa aacgctggcc gccgaggtgc
     1021 gcgcgatcct tcccgccgcc aagatcggct atggcgccga ctggtcggaa tacaacaacc
     1081 accagaccgg cgatgcgccc ggcgcgctgc gcttcaacct cgaccccttg tggagcgacg
     1141 ccaatatcga tttcgtcggc atcgacaatt acctgccgct ggccgactgg cgcgacggcg
     1201 ccgcccatct cgattacgac gcgcagaacg gccccacgag cattcacgat cccgcttatc
     1261 tcgcggcgaa catcaggggc ggggaagact tcgactggta ctatgcgagc gacgccgaca
     1321 gggcgtcgca aacccgcacg ccgatcaccg acggcctggg caagccttgg gtatggcgcg
     1381 ccaaggatct gtggagctgg tggtcgaatc cgcactatga ccgccccagc ggaagcgaaa
     1441 gcgccagcca caccgcttgg gtgccgcagt ccaagcccat ttggttcacc gagctgggct
     1501 gtccggcggt ggagaagggc gcgggccagc ccaacgtttt ctacgatccc aaatcgagcg
     1561 aaagcttctt tccctattat tcgaccggcg aacgcgacga cctcatccag cgccgcttcc
     1621 tcgaagcgca gttggatttc tggaaccggc ccgcgaacaa tcccgtctcc gacgtcacgc
     1681 acgcgccgat gcttgacctg tccaacctct atgtctggtg ctgggacgcg cggccgtttc
     1741 cgttctttcc cgcccgcgcc gacgtgtggg gcgacgccgc caactaccgc accggccatt
     1801 ggctgaacgg gcggctgggc gcggtgcaac tgtccgatct cgtcgccgcg ctgtgcgccg
     1861 agacgaattt cgcggagttc gacgcctcgg gtctcaccgg cctggtcacc ggctttgccg
     1921 tcaccgatac gatgagcgtg cgcgatgcgc tggcgccgct ctcgggcgct tatttcttcg
     1981 acggggtgga aagcgagggc ctgatcaagt tcgtcatgcg cgggcggccc gccgccttgc
     2041 cgctggccga aagcgacctc gcgctcgacg acggcgattc ctccttcggc ttcagcttcg
     2101 agcgtattca ggagaccgac ctgccgctgg cctcgcggat cacctatctc gacgccgatg
     2161 ccgattaccg ccaggccgtc gccgaggccc gccggctggt ggcgttgtcc gaccgtatcg
     2221 cgacgtcttc gctgccgctg gtgttcgacc agggccaggc catcggcatc ggccagcgcc
     2281 tgttgcagga cgcctgggtg atgcgcgaga gcgcgaagtt ctcccttggc ccctccaggc
     2341 tcgcgctcga tcccaccgac gaagtgctgc tggaggcggg cgggcgcacg cggcggctgc
     2401 gtctcaccgg catcgacgat gcgggcgcgc gccggatcga agcggtggcg accgatcctt
     2461 cggtgtacga aaccatcgtc ggctcggcgc gctcgacggc cgcgacgcag agcctgcgcc
     2521 agcctgggcg ggcgctggtc gtcttcctcg atctgccgct cttgacggac aaccagaatc
     2581 caaacgcgcc gctggtcgcc gcttatgccg atccctggcc cggcgcggtg caggtcctga
     2641 aaagcccgac ggattccaat tacgcgctcg acacttcggt cacgcgcccc gccacgctcg
     2701 gcaccaccct gtgggatttc tggtccggtc cgttgtggcg ctgggatcgc gtcaacacgc
     2761 tgtacgtcaa gctgttgagc ggcgcgctgg cgtcggcgga cgatgcttcg gtcttcgcgg
     2821 gggccaatgc gctcgccgtg cagaacgcgg acggcgaatg ggagatcgtc cagttcgcca
     2881 ctgccgagtt gaccgcgccg gacgaatggc ggctgaccag attgttgcgc ggccaacacg
     2941 gcagcgaagg ggcgatgcgc aaccccgtgg cggcgggcgc gcgcgtggtg gtgctggacg
     3001 aggcgctggc gcagttgagc ctcagccagg cgcaggcgcg ctggccgttc gcctatctgt
     3061 gggggcccgc cggcaagccg atctccgatc cggcgtttca aggcgcgact ctcgccttcg
     3121 aggccgccgg tctgatcccg cccgcgccgt gccatgtgcg ttttgcctgg tcggggaacg
     3181 atctgatctt ctcctggaag cgccgcgacc gcgcgtccgc cgccgcaaac ctgctccagg
     3241 cggaaacgcc gctgagcgag agcggcgaat tctacgacct tgaaatcctg aacggcggcg
     3301 cgctcgcgcg caccttctcc tctattcctc agcattcgca gatctacacc gcggcccagc
     3361 aggcggccga tttcccctcc ggccttccca atccgctggt ggtgaacgtg taccaacgct
     3421 cgtccgtgct cggccgcggg cggcaaaaga cggaatccct ctatgtccga tagcaccccg
     3481 cgcttgcgca tccccgaact cgtctcgatg caggaggcga acgccgtcac ctggaacgag
     3541 gcgctggtcc agctcgacgc cttcgtcgat ctttgtctgc tcggc
//
LOCUS       DASWPU010000396        13304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_113790,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000396 DASWPU010000000
VERSION     DASWPU010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13304
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113790"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VGC27_08510"
     CDS             <1..759
                     /locus_tag="VGC27_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY0282650.1"
                     /translation="GKNAAATFDNAVSGDPERTAPGKGGAPVSGTQDVAAPKTDANGN
                     DTPGQPRPVNTHQEAAPAQIAQSSAPGRSAGTPAPSAAAMVVQAQAQTASAASVYVNL
                     QVAPQHQDAIPTPTMDTLGLAIAAKSADGIKHFDIRMDPPELGRVQVHLSLDDAGKAQ
                     ASLVVDKPQTLELLQRDAASLTRSLADAGLSLSNNGLNFSLRGQDRQSDGGSVVKGRS
                     RSLSVKAVMGTDAISNSSSIGSLAPDSVRLDIRV"
     gene            781..1443
                     /locus_tag="VGC27_08515"
     CDS             781..1443
                     /locus_tag="VGC27_08515"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEY0282651.1"
                     /translation="MTTPVNATNSGAANSSSPPTSQQQLAGNFQTFLTLLTTQLKNQD
                     PTAPMDSTQFTQQLVMYSQVEQQIGTNTKLDSLISLGQSQFNNFSVSYLGKNVVVTDG
                     TASLREGAAEWTYALDGTAAATKLTVSDASGKTVFTKSGETAAGTHGFTWDGKDLNGN
                     QLNDGTYKLTVTSTAEDGSKIATSVASKDIVIGIDLSGSSPQLIIGTREVPLSSVTLI
                     TN"
     gene            1527..2864
                     /gene="flgE"
                     /locus_tag="VGC27_08520"
     CDS             1527..2864
                     /gene="flgE"
                     /locus_tag="VGC27_08520"
                     /inference="COORDINATES: protein motif:HMM:NF004242.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEY0282652.1"
                     /translation="MSLYGAMMTGVAGLSAFSNALSVSAANISNVNTVGYKTATSNFA
                     TLLASATSNSDPSSAGVVARAGQNVVQQGLMQTTNSPTDLAISGSGFFVVSQTPSASG
                     TKEYTRAGSFTTDESGFLKNTAGLYLMGWQLDSNGNVPSDRNDMTPINVNSLTGKAEA
                     STKMQLQANLKSSTAAIALYAPGDMADGTATPAFQRTINVYDSQGGSQPLQLSFVKTG
                     ANTWAYEVTYQGAAANLDAGGGTPPAHNLLASGTVTFNSDGSLADVVPTGGGAAPATG
                     SVSVDIPWSASSGLAAQTISINMGKVNGTDGITQYDNSSTMVSSTVDGALFGSLSGIS
                     VDANGIVTAQFSNGLSQAVYKIPLATFSNPNGLSEASGNAYLTSVASGSPTITEADLG
                     GAGTIQSKTLEGSTVDLASEFTNLITTQRAYSASAKIITTASSMLDDLLQMAR"
     gene            3029..3304
                     /locus_tag="VGC27_08525"
     CDS             3029..3304
                     /locus_tag="VGC27_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEY0282653.1"
                     /translation="MGEDRRGRVSYVIGPDGSPLTMADLPPPNTKRWVIRRKAEVVAA
                     VRGGLLSLDEACRRYTLTVEEFLAWQRAIDRFGLPGLRATRVQQYRN"
     gene            3497..5134
                     /gene="fliF"
                     /locus_tag="VGC27_08530"
     CDS             3497..5134
                     /gene="fliF"
                     /locus_tag="VGC27_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEY0282654.1"
                     /translation="MPDFVKAIGAARFGVMAGIAAALTAFFLYIAGVISEPPKSILFS
                     GLESRDAAAVSAKLDALNVPYDAKGDGGTIMVPADQVTKLRMELARDNLPAVGVGYEI
                     FDKSDAFGTTAFVQNINRLRALEGELARSIQTIAGVESVRVHLVIPERQIFSRDAQAP
                     SGSVVLKTRGVLGREQVGAIQHLVAASVAALTPERVAIVDDKGNLLAGGQDMSGDDAF
                     SGRTEQNTTDFEDRMRRRIESMVASIVGQGHVRAQVAAEMNFTRTTSTSEDFNPDSKV
                     VRSTQTVEQNASDSSAAGGAVSIASALPGQQNASGGDNGKSSSGRTEETTNYEISKTV
                     TTSATEGKDGSTLKRLSVAVVVDGITAEGGAYKARSAQEMKQIDMLVKSTIGYDAARG
                     DQIQVVNMPFARVETIAGTPAPTPLLGLDGAAWFKIIEAAILSLTALLIGLFVMRPLI
                     ARMFAPVSFAAGPGQIASGTPAAGQLAAPAAQGAEAQAPAGHASAQIPPPRENMIDIS
                     RIEGQVRESSVKKVGEVVQSHPEEALAIMRTWLHQPV"
     gene            5141..6175
                     /gene="fliG"
                     /locus_tag="VGC27_08535"
     CDS             5141..6175
                     /gene="fliG"
                     /locus_tag="VGC27_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEY0282655.1"
                     /translation="MSRALKATVKEDIRSLSGAERTAILMLSLGEEHSAKLWQMLDED
                     EIKEISQIMSNLGTVSSSLVEKLLVEFVSQMSGTGSLMGSYESTERMLARIMPQEKVS
                     QIMEEIRGPAGRTMWDKLANVNETVLANYLKNEYPQTVSVVLSKIKPEHAARVLTALP
                     EEFALEVVQRMLRMESVQKDILDKVERTLRVEFMSNLARTAKRDAHEHMAEIFNNFDR
                     QTESRFVTALEERNRDSAERIKALMFTFEDLGKLDPGSIQTLLRHVEKDKLGLALKGA
                     TDSLRDVFFSNMSERAGKILREDMEAMGPVRLKDVDEAQMRMVNVAKDLANKGEIMIA
                     NKQGEDELVY"
     gene            6175..6819
                     /locus_tag="VGC27_08540"
     CDS             6175..6819
                     /locus_tag="VGC27_08540"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEY0282656.1"
                     /translation="MSASAKPVSKFTFDTEFRAEGDLISNAARARQRKVFTQEEIDQM
                     CAKAREMGLKAGQVRAAEAAAAAIADLAKEVRAALAGSHDEIEIMREEAAQIALAAAR
                     KFAPAAIAALPCGDVEATLREAMHQAIGEPRIVLRASPAVSQAIAESLAAIAHEEGFE
                     GRIVAKADPAITGADCRVEWRGGGAERSLSALEETIGELIARRFAQTDKTTTKG"
     gene            6823..7182
                     /gene="fliN"
                     /locus_tag="VGC27_08545"
     CDS             6823..7182
                     /gene="fliN"
                     /locus_tag="VGC27_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEY0282657.1"
                     /translation="MAGNDDVDLPDLSHDAIDESAPALLTGESAADVDVKRSAQDLEA
                     VFDVPVTVSAVLGKSAMEVSHLLKLSKGTIVELDRKVGEAIDIYVNDRLVARGEVVLV
                     EDRLGVTMTEIIKAGQS"
     gene            7285..8658
                     /locus_tag="VGC27_08550"
     CDS             7285..8658
                     /locus_tag="VGC27_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0282658.1"
                     /translation="MRLLIVGGLQGQIGAATKIAMDRGAKVTHATDIEQGLASLRGGS
                     GADLVLCDICCDIGTLVRALAAERICTPVVACGVGTDARKAVDAIRAGAKEYLPLPPD
                     PELIAAVLAAVADETHQMVYEDEAMTAVITLADQVAGSEASILITGESGSGKEVLARY
                     VHRKSARADKPFISVNCAAIPENLLESELFGHEKGAFTGAIARRIGKFEEANGGTLLL
                     DEISEMDARLQAKLLRAIQEREIDRVGGTKPVKVDIRIVATSNRDLADAAKRGTFRED
                     LLYRLNVVNLRLPALRERPKDIQALARHFARKYAEANGVPFRAIAPATERLLASHPWR
                     GNVRELENTIHRAVLLATGTEIGPEAIRLPDGTQVGGAGLADVPDHVRSAVESAAAVT
                     RSLVGRTVADVERDLILDTLDHCLGNRTHAANILGISIRTLRNKLREYAQAGLAVAQP
                     GEQRMTG"
     gene            8833..10944
                     /gene="flhA"
                     /locus_tag="VGC27_08555"
     CDS             8833..10944
                     /gene="flhA"
                     /locus_tag="VGC27_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEY0282659.1"
                     /translation="MADVSTTPARTGFDFSLAGIADFVKRSDLALALGIMAILVVLIL
                     PLPTWLLDISLAFSLSFSILILMTAVFVRKPLEFSSFPAILLITTMMRLALNLASTRL
                     ILAHGNTGTAAAGYVIEAFGKLIMQGNFVIGLIVFSILVVVNFVVITKGSGRIAEVAA
                     RFSLDAMPGKQMAIDADLSAGLIDENDARKRRKDLEAESNFFGAMDGASKFVRGDAIA
                     GLLIVGINVIGGIIVAVVQHGMSFADASHTFTLLSVGDGLVSQMPALIVSTAAGLMVS
                     KAGVEGSTDRALMRQLSFYPQALGMASAVMGIVAVLPGMPTLAFAGLSAGVGGLAWSA
                     YRKKNTEEIKTRVEEEKAKADAAPTEEPIATALALDLLRIELGYGLLPLINDVQGHRI
                     TDQIKALRRQLAQEMGFVMPAVRILDNMQLGANEYRISVKEVDSGRGELIPGSLLVMD
                     PKGQPIDLPGTHTTEPAFGLPATWVTGSLREEASFRGYTVVEPGTVLTTHLTEVLKAH
                     MSELLSYAETKKLLDDLPAEHKKLVEELIPSQISVTGVQRVLQTLLAERVSIRDLTAI
                     LEGIAEAVGHTKNALYITEHVRARLARQLCHANLSPGGYLPILALSPAWEQAFAESII
                     GQGEDRQLAMAPSQLQQFITSVRDRFEEASQKNEVPVLLTSPQIRPFVRSIVERFRTQ
                     TVVMSQNEIHASIRLRTLGQV"
     gene            11038..12063
                     /locus_tag="VGC27_08560"
     CDS             11038..12063
                     /locus_tag="VGC27_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282660.1"
                     /translation="MQLRTFLARDMKAALASVRAEMGPEAVIVGSERVKSGIMVRAAL
                     EAPETPDLAEEIGEPVNDFDADYHDALIRRLREKPATIPGRRRFDRSELLASFARHRL
                     PDALGHALAEASAKTNVSDMTLALAAAIDARMRSVPIDFAAAKAFMLVGPNGAGKTAV
                     AAKLAAHAKLAGRNVALIAGDVAGAGAVARLNAFAEHLDAAISTADSAFVLADTTGEA
                     VAQGTLAIIDTAGFDPRQLKIAAVYAALAKIESVEAIGVLSALTDAEEAAEIAAALAR
                     IGAGRLIITAADLARRAGALAAAAMTPDVGLAHITRSPYVAGSLETLTPLALSRLLAE
                     GEQESAQ"
     gene            12060..12857
                     /locus_tag="VGC27_08565"
     CDS             12060..12857
                     /locus_tag="VGC27_08565"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY0282661.1"
                     /translation="MTLKSLGPRLTAIGSGKGGTGKTFVAVALAQTFADLGERVLLCD
                     ADLGLANAAVQLGLASGGDLPGLLAGRIVLADAVAPVETSSHASFDLLSAPAGSGALA
                     DTDAETAERLAAQLKRAPGYDRVLIDLGAGVGASAMTLAAHADDTLVLTTPDPASLTD
                     AYAFAKLMLKRTGGRAPAIVVNMAAGTGEAKRTADALIRSAQTFLKIAPAYLGFVPSD
                     VRVVEAVRRQTMPCALYPQSPAVAAIAALASALGGQIGNPQIATSLR"
     gene            13011..>13304
                     /locus_tag="VGC27_08570"
     CDS             13011..>13304
                     /locus_tag="VGC27_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282662.1"
                     /translation="MSALFEIWHSIQGILTNADWITLALIALVAIIGGFAIQGINSIL
                     GATVLGLIGFVLLVFVRAVTIGKQDFTAYTSTGWHDVLNLPTLTLLSYFIIFAV"
BASE COUNT         2582 a         4401 c         4113 g         2208 t
ORIGIN      
        1 ggcaagaacg ccgccgcaac tttcgataat gcagtgtccg gcgatcccga acgaaccgct
       61 cccggcaaag gcggcgcacc tgtgagcggc acacaagatg ttgctgcgcc caagacagat
      121 gcgaacggga acgacacgcc ggggcagcca cggccggtaa acacgcatca ggaggcggca
      181 ccggcgcaaa ttgcgcaatc gtccgccccg ggacgatcgg ccggcacgcc cgcgccttcc
      241 gctgcggcaa tggttgtcca ggcgcaggcc caaaccgcaa gcgccgcttc cgtttacgtc
      301 aatcttcagg ttgcaccgca gcaccaagat gcgatcccga cgcccacgat ggacacgctc
      361 ggtttggcca tcgccgcgaa atccgcggat ggaatcaagc atttcgacat tcgcatggat
      421 ccgcccgaac tcggccgcgt ccaggttcat ctctcgctcg acgacgcggg caaggcgcag
      481 gcaagccttg tggtcgacaa accgcagacc ctcgaattgc tgcagcgcga cgccgcgagc
      541 ctcacccgct cgctcgcaga cgcgggcctc agcctgtcga acaatggcct caatttctcg
      601 ctaagaggac aggaccggca gagtgacggc ggcagcgtgg tcaaggggcg cagccgctcg
      661 ctgtcggtga aggccgtcat gggtacggac gcaatctcga attccagttc catcggcagc
      721 ctcgcgcccg acagcgtgcg gctggacatc cgcgtatagg acgtaaaagg aagcactgcc
      781 atgaccactc ccgtgaacgc aaccaattca ggcgcagcga actccagttc gccgccgacc
      841 tcccagcagc agcttgccgg caacttccag accttcctga cgttactgac gacgcagttg
      901 aagaatcagg acccgacggc gccgatggat tccacccagt tcacgcagca gttggtcatg
      961 tacagccagg tcgaacagca gatcggcacc aacaccaagc tcgattctct gatctcgctc
     1021 ggccagagcc aattcaacaa tttctccgtg agctatctcg gcaagaacgt cgtggtgacc
     1081 gacgggacgg cgtcgttgag agaaggcgcg gcggaatgga cttacgctct cgacggaacc
     1141 gccgccgcga ccaagctgac ggtgtcggac gcgagcggca agaccgtctt caccaagagc
     1201 ggcgaaaccg ccgcgggcac acacggtttc acttgggacg gcaaggacct taacggcaac
     1261 cagctcaacg acggcactta caaattgacc gtcacctcga ccgccgagga cggatcgaag
     1321 atagcgacct ccgtggccag caaagacatc gtcatcggaa tcgacctgtc gggctcgagc
     1381 ccgcaactca tcattggaac gcgggaagtg ccgctctcga gtgtgaccct catcaccaac
     1441 tgatcgcaac cgcggccgag aaacggctgc actctctgaa ggacggcaac ggggcgaagc
     1501 ccaaaacgcc gagaaggaag aaacaaatga gtctctatgg cgcaatgatg accggtgttg
     1561 cgggcctgag cgcattcagc aatgccctta gcgtctccgc ggcgaacatc tcgaacgtca
     1621 acaccgtcgg ctacaagacg gccacgagca atttcgcgac gctgctcgcg tccgccacca
     1681 gcaacagcga tccgtcgtcg gcgggcgttg tcgccagagc ggggcagaac gtcgtccaac
     1741 agggcttgat gcagacgacc aactcgccga ccgatcttgc catttcaggc agcggcttct
     1801 tcgtcgtctc gcaaaccccg tcggcttccg gaacgaagga atatacccgc gccggcagct
     1861 tcacgaccga cgagagcggt ttcctcaaaa acacggcggg gctttatctg atgggctggc
     1921 agctcgacag caacggcaac gtcccttccg accgcaacga catgacgccg atcaacgtca
     1981 acagcctgac gggcaaggcc gaagcctcga cgaagatgca gctgcaagcc aatctcaaat
     2041 ccagtaccgc ggcgattgcg ctgtacgctc caggggacat ggcggacgga accgcgacgc
     2101 cggcgttcca gcgcacgatc aatgtctacg acagccaggg cggttcccag cccctgcaac
     2161 tgtcgttcgt gaagaccggc gcgaatacct gggcgtatga agtgacctac cagggcgccg
     2221 cggcgaatct cgacgccggc ggcggcacgc ctccggccca caatcttctg gcctccggca
     2281 cggtgacctt caattcggac ggctcgctgg ccgacgtggt gccgaccggc ggcggggctg
     2341 cgcccgctac gggaagcgtc tcggttgata ttccgtggtc ggcctcctcc ggcctggccg
     2401 cccaaacgat ctccatcaac atgggcaagg tcaacggaac ggacggcatc acccaatacg
     2461 acaattcctc gaccatggtg tcgtcgacgg tggacggcgc gttgttcggc agcctgtccg
     2521 gcatctccgt cgacgccaac ggcatcgtca cggcgcagtt ctccaatggc ttgtcgcagg
     2581 ccgtgtacaa gatacccctg gcgaccttct ccaatccgaa cgggttgtcg gaggcttcgg
     2641 gcaacgccta cttgacctcg gtggcgtccg gttcgccgac catcaccgaa gccgatctgg
     2701 gcggcgccgg aacgattcag tcgaagacgc tcgaaggctc caccgtcgat ctcgccagcg
     2761 agttcaccaa tctgatcacg acgcagcgtg cctattctgc ctcggcgaaa atcatcacga
     2821 cggcatcgtc gatgctggac gatcttctgc agatggcgcg ttaataaaaa atttccagcg
     2881 cctttaccca gtaacggcgc tggaaattct tgaatagatg caaatctacg ggtcgtttta
     2941 gaaggtcgtt taccgtgcct atagaccagt tttttaaggc gtcacgggta ccttgcccac
     3001 gggtagtgga gtcaggagta cgatgaacat gggcgaagac cgtagaggtc gcgtgagcta
     3061 cgtcatcggg ccggatggca gtccgttgac gatggccgat ctgccgcctc cgaacacgaa
     3121 gcgttgggtg atacgccgca aggccgaagt tgtcgccgcc gtacgcggcg gacttctcag
     3181 cctggatgaa gcgtgccgcc gttatacgct gacggttgag gagttcctcg cctggcagcg
     3241 tgctatcgac cgcttcggct tgccgggtct gcgtgcgacg cgcgtccagc aatacaggaa
     3301 ctgacaggcg aaaatcggaa atcggaaaga aattgttaat tccgtttccc gaattccgaa
     3361 atttgatttt cggcttccga ctccggaaag ctcgccccat taaggcgaat tttaccaagc
     3421 acgcggcaat gtttgcctag cacggcggac agcgggacgg cctcccactc tccgccagta
     3481 tgcgggggag caggcattgc cggatttcgt caaagcaatc ggagccgcac ggttcggcgt
     3541 catggcgggc atcgccgcgg cgctcaccgc ctttttcctc tatatcgcgg gcgtgatttc
     3601 cgagccgccc aaaagcatcc tgttttcggg tctcgaatcg cgcgacgccg ccgcggtcag
     3661 cgccaagctc gacgccctca acgtgcccta cgacgccaag ggcgacggcg gcacgatcat
     3721 ggtccccgcc gaccaggtca ccaagctgcg catggaactg gcgcgggaca atctgcccgc
     3781 ggtcggcgtc ggctacgaaa tcttcgacaa gtccgacgcc ttcggcacca ccgctttcgt
     3841 gcagaacatc aaccggctgc gcgcgctgga aggcgaactg gcgcgctcga tccaaaccat
     3901 cgcaggcgtc gagagcgtcc gcgttcatct cgtcattccc gaacgccaga tcttctcgcg
     3961 cgacgcgcag gcccccagcg gttcggtcgt gctcaagacg cgcggcgtgc tcggccgcga
     4021 acaagtgggc gccatccagc atctggtcgc cgcctccgtc gcagcgctga cgcccgagcg
     4081 cgtcgccatc gtcgacgaca aaggcaatct gctggcgggc ggccaggaca tgtccggcga
     4141 cgacgccttc agcggccgga cggaacagaa caccacggat ttcgaggacc gcatgcgccg
     4201 gcgcatcgaa tccatggtgg cgagcatcgt gggccaaggc catgtccgcg cccaggtcgc
     4261 cgccgagatg aacttcacgc gcacgacctc gacctccgag gacttcaatc ccgacagcaa
     4321 agtggtgcgt tccacccaga ccgtcgagca gaacgccagc gacagcagcg ccgccggcgg
     4381 cgccgtttcc atcgccagcg cgctgcccgg ccagcagaac gcgagcggcg gcgacaacgg
     4441 caaatcgagt tccggccgca ccgaagaaac caccaactac gaaatctcca agacggtgac
     4501 caccagcgcg accgagggca aggacggcag tacgctcaag cggctgtcgg tcgccgtcgt
     4561 ggtcgacgga atcaccgccg agggcggtgc ctacaaggcg cgcagcgcgc aggagatgaa
     4621 acagatcgac atgctggtga agtccaccat cggttacgac gcggcgcgcg gcgatcagat
     4681 ccaggtcgtc aacatgccgt tcgcccgcgt ggagacgatt gccggaacgc cggcgccgac
     4741 gccgctgctg ggtctcgacg gagccgcctg gttcaagatc atcgaagcgg ccatcctgtc
     4801 gctgaccgcg ctgctcatcg gcctgttcgt catgcgtccc ttgatcgcac gcatgttcgc
     4861 gccggtctcg ttcgcggcgg ggcccggcca gatcgcttcc ggcacgcccg ccgcgggtca
     4921 actggccgcg cccgccgcgc aaggcgccga ggcgcaggcg ccggcgggtc acgcctccgc
     4981 gcagattccg ccgccgcgcg aaaacatgat cgacatcagc cgcatcgaag gacaggtgcg
     5041 cgaatcctcg gtaaagaaag tcggtgaagt cgtgcagtcc catccggaag aagcgctggc
     5101 gatcatgcgt acttggctcc accaaccggt gtaacgaacg atgtcccgcg ccctcaaagc
     5161 aaccgtcaaa gaagacatcc gctcgctgtc cggcgcggag cgcaccgcca tcctcatgct
     5221 ttcgctgggc gaagagcatt cggccaagct ctggcagatg ctcgacgagg acgagatcaa
     5281 ggaaatttcc cagatcatgt ccaatctggg cacggtgagt tcgagcctgg tcgagaagct
     5341 gctggtcgaa ttcgtctcgc agatgtcggg caccggctcg ctgatggggt cctacgaatc
     5401 caccgagcgg atgctggccc gcatcatgcc gcaggaaaaa gtcagccaga tcatggagga
     5461 aatccgcggt ccggccgggc gcaccatgtg ggacaagctc gccaacgtca acgaaacggt
     5521 gttggcgaac tacctcaaga acgaatatcc gcagaccgtc tccgtcgtgc tctccaagat
     5581 caagcccgaa cacgccgcgc gcgtgctgac ggcgctgccc gaggagttcg cgctcgaagt
     5641 cgtgcagcgc atgctgcgga tggaaagcgt gcagaaagac atcctcgata aggtggaacg
     5701 caccttgcgc gtcgagttca tgtccaacct ggcgcgcacc gccaagcgcg acgcccacga
     5761 gcacatggcg gaaatcttca acaatttcga ccgccagacc gaaagccgct tcgtcaccgc
     5821 gctggaagag cgcaaccgcg actcggccga gcgcatcaag gcgctgatgt tcaccttcga
     5881 ggatctgggc aagctcgatc cgggctcgat ccagacgctg ctgcgccatg tcgaaaagga
     5941 caaactaggg ctggcgctca agggggccac cgattccctg cgcgacgtct tcttctccaa
     6001 catgagcgag cgcgccggaa aaatcctgcg cgaagacatg gaggccatgg gtccggtgcg
     6061 gctcaaggac gtcgacgaag cgcagatgcg catggtcaac gtcgccaagg acctcgccaa
     6121 caagggcgag atcatgatcg ccaacaagca gggcgaagac gagctggtgt attgatgagc
     6181 gcgagcgcta aaccggtcag caaattcact ttcgacacgg aattccgggc tgaaggcgat
     6241 ctgatttcga acgcggcgcg ggcgcgccag cgcaaggtct tcacccagga agaaatcgac
     6301 cagatgtgcg ccaaggcgcg cgagatgggc ctcaaggccg gtcaggtgcg cgccgccgag
     6361 gcggccgccg ccgcgatcgc agatcttgcc aaggaggttc gcgcggcgct cgccggctcg
     6421 catgacgaaa tcgaaatcat gcgcgaggag gcggcgcaga tcgcgcttgc cgccgcccgc
     6481 aagttcgcgc ccgccgccat cgccgccctg ccttgcggcg acgtcgaggc gacgctcagg
     6541 gaagccatgc accaagccat cggcgaaccg cgtatcgtgc tgcgcgcctc cccggccgta
     6601 agccaggcga ttgcggaaag tctggcggcg attgctcatg aagaaggctt cgagggccgc
     6661 atcgtcgcca aggccgaccc tgccattacc ggcgccgatt gccgcgtcga atggcgcggc
     6721 gggggcgccg agcgcagcct gagcgcgctc gaagagacga tcggcgagct gatcgcgcgg
     6781 cgcttcgcgc aaaccgacaa gacgacgacg aaaggataaa ccatggcggg aaacgacgac
     6841 gtggatctgc ccgatctctc ccatgacgcc atagacgaaa gcgcccccgc tctgttgacc
     6901 ggcgagtccg ccgccgacgt ggacgtcaag cgctcggccc aggatctgga agcggtgttc
     6961 gacgtgccgg tcacggtttc ggccgtgctc ggcaaatccg ccatggaagt cagccatctt
     7021 ctcaagctca gcaaaggcac tatcgtcgag ctcgaccgca aagtcggcga agccatcgac
     7081 atttacgtca acgaccgtct ggtcgcgcgc ggcgaagtcg tcctggtcga agaccggctg
     7141 ggcgtcacca tgacggaaat catcaaggcc ggtcagtcgt gaccaagttt tcaccctcac
     7201 ccttcgtcaa gctcagggtg agggcatcgc atttaacctt catgctgagc ctgtcgaagc
     7261 atgaaggtgg cacgggagaa taacatgcgg ctactcatcg tcggcggact gcaaggccag
     7321 atcggtgcgg caaccaagat cgcgatggac cgcggcgcca aggtcacgca cgcgaccgac
     7381 atcgaacagg gtctcgccag cttgcgggga ggcagcggcg cggacctcgt gctctgcgac
     7441 atctgttgcg acatcggcac gctggtgcgg gcgctggcgg ccgaacgcat ctgcacgccg
     7501 gtggtggcct gcggcgtggg gaccgacgcg cgcaaggcgg tggacgccat ccgcgccggg
     7561 gccaaggaat atctgccgct gccgcccgat cccgaattga tcgccgcggt gctggccgcc
     7621 gtcgccgacg aaacccatca gatggtctac gaggacgagg cgatgacggc cgtcatcacc
     7681 ctcgccgatc aagtggcggg aagcgaagcc tccatcctga ttaccggcga aagcggcagc
     7741 ggcaaggaag tgctggcgcg ctacgtccac cgcaaatcgg cccgcgccga caagcccttc
     7801 atctcggtca actgcgcggc gatcccggag aatttgctgg aatccgaatt gttcggccat
     7861 gagaagggcg cgtttaccgg cgccatcgcg cggcgcatcg gcaagttcga ggaagccaat
     7921 ggcggcaccc tcctgctcga cgaaatcagc gagatggacg cgcgtctgca agccaagctc
     7981 ttgcgcgcca tccaggagcg cgagatcgat cgcgtcggcg gcaccaagcc ggtcaaggtc
     8041 gatatccgca tcgtggcgac atcgaaccgc gatctcgccg acgcggccaa gcgcggcacc
     8101 ttccgcgaag acctgcttta ccgtttgaac gtcgtcaacc tgcgcctgcc ggcgctgcgc
     8161 gaacggccca aggacatcca ggccctggcg cggcacttcg cgcgcaaata cgccgaagcc
     8221 aatggcgtgc ccttccgcgc catcgccccg gcgacggaac ggctgctggc ctctcatccc
     8281 tggcgcggca atgtgcgcga gttggagaac accatccacc gcgcggttct cttggccacc
     8341 ggcaccgaga tcgggcccga agccatccgc ctgcccgacg gcacccaggt cggcggcgcc
     8401 ggcctggccg atgttcccga tcatgtgcgc tcggcggtgg aatcggcggc cgccgtcacc
     8461 cgcagcctgg tcggacgcac cgtcgcggac gtggagcgcg atctgatcct cgataccctc
     8521 gatcactgcc ttggcaaccg cacccatgcg gcgaacattc tcggcatctc catccgcacg
     8581 ctgcgcaaca agctgcgcga atatgcccag gccggactcg cggtcgccca gccgggcgaa
     8641 cagagaatga caggatgagg atgaaaccgg aggcgacatt cctcttcgcc tcctcctttg
     8701 aggggggcga gacgcggcgg cataggagcg aaaaatccgc acagcggatt tttcggggaa
     8761 gggtgacatc gacatcaccc ctctccgaac ggccttcggt cgttcgaccc tccctcaagg
     8821 ggagggtgag aaatggctga cgtctccacc acgccggccc gcacgggctt cgatttttct
     8881 ctcgccggca tcgccgattt cgtcaagcgc agcgatcttg cgctggcgct cggcatcatg
     8941 gcgatcctgg tggtgctgat cctgcccttg ccgacctggc ttctggatat cagcctcgct
     9001 ttttccttgt ccttttccat tctgatcctg atgacggcgg tgttcgtccg caagccgctg
     9061 gagttcagct cctttccggc gatcctgttg atcaccacga tgatgcgtct ggcgctcaac
     9121 ctcgcctcga cgcgcctgat cctggcccac ggcaacaccg gtaccgccgc ggcgggctat
     9181 gtgatcgaag ccttcggcaa gctgatcatg cagggcaatt tcgtcatcgg cctgatcgtc
     9241 ttctcgatcc tggtggtggt gaatttcgtg gtcatcacca aaggttcggg gcgcatcgcc
     9301 gaagtcgcgg cgcgcttcag cctggacgcc atgcccggca agcagatggc gatcgacgcc
     9361 gatctgtcgg ccggactgat cgacgagaat gacgcacgca agcgccgcaa ggacctggag
     9421 gcggaatcga atttcttcgg cgccatggac ggcgccagca aattcgtgcg cggcgacgcc
     9481 atcgccggcc tgttgatcgt cggcatcaac gtcatcggcg gcatcatcgt cgcggtggtc
     9541 cagcacggca tgagtttcgc cgacgccagc cacaccttca ccctgctttc cgtcggcgac
     9601 ggcctggtca gccagatgcc ggcgctgatc gtttccacgg cggcgggcct gatggtgtcg
     9661 aaggcgggcg tcgaaggctc caccgaccgc gccctgatgc gccaattgtc cttctatccc
     9721 caggcgctgg gcatggcctc ggcggtgatg ggcatcgtcg ccgttctgcc cggcatgccg
     9781 actctcgcgt tcgccgggct ttcggcgggc gtcggcggtc tggcctggag cgcctacagg
     9841 aagaagaaca ccgaagaaat caaaacccgt gtcgaagagg aaaaggccaa agccgacgcc
     9901 gcgccgacgg aagaacccat cgccaccgcg ctggcgctcg atctcttgcg catcgaactg
     9961 ggctacggcc ttctgccgct catcaacgac gtccagggcc accgcatcac cgaccagatc
    10021 aaggcgctgc gccgccagct cgcccaggag atgggcttcg tcatgccggc cgtccgcatc
    10081 ctcgacaata tgcagctcgg cgccaacgag taccgcatca gcgtcaagga agtggattcg
    10141 gggcggggcg agcttatccc gggcagcctt ctggtcatgg atcccaaggg ccagccgatc
    10201 gaccttcccg gcacccacac gacggaaccg gccttcggcc tgcccgccac ctgggtgacc
    10261 ggttcgctgc gcgaggaagc cagcttccgc ggctatacgg tggtcgagcc cggcacggtg
    10321 ctgacgacgc atctgaccga agtgctgaag gcgcacatgt ccgagctctt gtcctatgcc
    10381 gaaaccaaga agctgctcga cgacttgccc gcggagcaca agaagctggt cgaagagctg
    10441 atcccctcgc agatttcggt caccggcgtg cagcgcgtat tgcagacgct gctggccgag
    10501 cgcgtttcca tccgcgacct caccgcgatt ctggaaggca tcgccgaagc cgtcggccac
    10561 accaagaacg cgctctacat cacggagcat gtccgcgccc gtctggcgcg ccagctttgc
    10621 cacgccaacc tctcgcccgg cggctatctg ccgatcctgg ctttgtcgcc ggcctgggaa
    10681 caggcgtttg cggaatcgat catcggccag ggcgaggacc gccagctcgc catggcgccg
    10741 tcgcagcttc agcagttcat caccagcgtg cgcgaccgct tcgaagaggc cagccagaag
    10801 aacgaagtgc cggtgctgct gaccagcccg cagatcagac ctttcgtgcg ctcgatcgtc
    10861 gagcgcttcc gcacccagac cgtggtgatg tcgcagaacg aaatccacgc ttcgatccgt
    10921 ttacggactt tgggacaagt ctgatggcag tagcgcagat gtcaaaacat ggatggccgg
    10981 gtcggttcgt acgctgttgc tacgaaccgc tgcccggcca tgacgaggtt taagggaatg
    11041 cagctgcgca ccttcctcgc cagggacatg aaggccgcac tggccagcgt gcgcgccgag
    11101 atggggcccg aagccgtcat cgtcggcagc gagcgcgtca agagcggcat catggttcgc
    11161 gcggcgctcg aagctcccga aacgccggac ctcgccgagg aaatcggcga gccggtcaac
    11221 gatttcgacg ccgattacca cgacgcgctg atccgccgct tgcgcgaaaa gccggcaacg
    11281 ataccaggcc ggcggcgctt cgaccgcagc gagcttctgg cctcgtttgc gcgacatcgc
    11341 ctgcccgatg cgctgggaca cgccctcgcc gaagccagcg ccaagaccaa tgtgagcgac
    11401 atgacgctgg cgctcgccgc cgccatcgat gcgcggatga ggtccgtccc gatcgacttc
    11461 gccgccgcca aggcgttcat gctcgtcggt cccaacggcg ccgggaaaac ggcggttgcc
    11521 gccaagctag cggcgcatgc caaacttgcc ggccgcaatg tcgctctgat cgccggcgat
    11581 gtcgcgggcg cgggcgcggt ggccaggctc aacgcctttg ccgaacatct cgacgccgcc
    11641 atctcgaccg ccgattccgc tttcgtcctg gccgacacga ccggcgaagc cgtggcgcag
    11701 ggcacgctcg ccatcatcga taccgccggg ttcgatccgc gccagctcaa aatcgccgcc
    11761 gtctatgcgg cgctggccaa gatcgagtcg gtggaggcca tcggcgtcct gtcggcgctg
    11821 accgacgcgg aagaagccgc cgagatcgcc gccgcgctgg cgcgcatcgg cgcggggcgc
    11881 ctgatcatca ccgccgccga tctggcccgg cgcgcgggtg cgctggccgc cgccgcgatg
    11941 acgccggacg tcggccttgc ccatatcacc cggtcgccat acgtggccgg cagtctggaa
    12001 acgctcacac ccctggcgct gtcgcgcctt cttgccgaag gcgaacagga gagcgcccaa
    12061 tgacgctgaa atccctgggc ccgcgcctga ccgccatcgg ttccggcaaa ggcggaaccg
    12121 gcaagacctt tgtcgccgtg gcgctggcgc aaacctttgc cgatctgggc gaacgcgtgc
    12181 tgctctgcga tgccgatctg ggccttgcca acgccgcggt ccagcttgga ctggcgagcg
    12241 gcggcgattt gccgggactg ctggcgggaa gaatcgttct ggccgacgcg gtcgcgccgg
    12301 tcgagacaag ttcgcatgcc agcttcgatc tgttgtcggc gccggccggc agcggtgcgc
    12361 tggccgatac cgacgccgag acggccgaac gcctggcggc gcagcttaaa cgcgctcccg
    12421 gctatgaccg cgtgctgatc gatctcggcg ccggcgtcgg cgctagcgcg atgactcttg
    12481 ccgcccatgc cgacgacacg ctggtgctga cgacacccga ccccgcctca ctcaccgacg
    12541 cctatgcttt cgccaaactg atgctcaagc gcaccggcgg acgtgcgccc gcgatcgtcg
    12601 ttaacatggc ggccggaacc ggcgaagcca aacgcaccgc cgatgcgctc attcgctcgg
    12661 cgcagacttt cctgaagatt gcccccgcct atctgggatt cgtgcccagc gatgtgcgcg
    12721 tggtcgaggc cgtgcgccgc cagaccatgc cgtgtgcgct ttatccacag agccctgccg
    12781 ttgctgcaat tgcggcgttg gcgagcgccc ttggcggcca aataggcaat ccgcaaatcg
    12841 cgacgtcgct tcgttaacta taaatttcca gagaagccta tcacttcgca cgttttctca
    12901 gcgacgcgga attggtgtat gatgtgttaa taggcattgt cggaggcacg cgtttcggcg
    12961 cgtcgaagcg cgttcgctgt gggaaaagca cattcgttca agggggtaaa atgtctgcgt
    13021 tattcgaaat ctggcactcg atccaaggca tcctgacaaa cgccgactgg atcacgctcg
    13081 cgctcatcgc cctggtggcg atcatagggg gattcgctat ccagggcatc aattcgatcc
    13141 tgggcgcgac agttctgggg ttgatcggct tcgtgctctt ggtctttgtc cgcgccgtga
    13201 cgatcggcaa gcaggatttt acggcttaca cgagcaccgg ctggcacgac gttctcaact
    13261 tgccgacgct gacgctgttg tcctacttca tcattttcgc ggtg
//
LOCUS       DASWPU010000397         7988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_27576,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000397 DASWPU010000000
VERSION     DASWPU010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27576"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VGC27_08575"
     CDS             complement(<1..302)
                     /locus_tag="VGC27_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0282663.1"
                     /translation="MTSAGSTAKVELRGVKKRFGRKIVLDGVDLTIQPGQSLVVIGGS
                     GTGKSVMIKCVLGILRPDAGEIFVDGKDVTRLRGRSRDTVLKKFGMLFQGAALFDS"
     gene            complement(314..1081)
                     /locus_tag="VGC27_08580"
     CDS             complement(314..1081)
                     /locus_tag="VGC27_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0282664.1"
                     /translation="MNVFAAIGRVSLALLGEIGRLAVFTLDAVTACFRPPIYWSLVLK
                     QMMRIGYFSLPVVGLTAFFTGGALALQIYTGAARYGAEAFVPVVVMVGITRELGPVIA
                     GLMVAGRVAAAIAAELGTMRVTEQIDALTTLATNPIKYLVVPRLAAAVVSMPILVAVA
                     DSIGILGGYIVSTQSLGFTGSIYLKNTVDAMTLHDVSSGLIKAAAFGFIIALMGCFNG
                     FHSKGGAQGVGAATTNAVVSASILILAANYLLTNLLF"
     gene            complement(1078..2199)
                     /gene="alr"
                     /locus_tag="VGC27_08585"
     CDS             complement(1078..2199)
                     /gene="alr"
                     /locus_tag="VGC27_08585"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0282665.1"
                     /translation="MDRTDEPRIADFEAARLVVRLGAIAANFKTAQRLAGTATAAAAV
                     KADAYGTGLSPVASVLVHAGCDTFFVARLEEGLALRPLAPEARIFVLDGAQPDAVPAL
                     ISHRLTPVLNSIAEIAGWSAAARATRTRLDAAIHIDTGMNRLGLPGAELATLAADHAK
                     RLDGLRLVLVMSHLACSDDSEAKMNRIQLGRFKTALAMLPPAPASLAASGGILLGREF
                     LFDMVRPGLCLYGGNPQPAKPNPFQVAVRMTARILQLHRADKGETVGYGATFRTRRPS
                     LLATAGVGYADGLMRAVSSRGSGAVGGVRVPVVGRVSMDLVTLDVTDVTGSLAAGMEV
                     EFFGDTIPLEEIAVAANTISYEILTSLSHRATRVYEAAP"
     gene            complement(2274..2891)
                     /locus_tag="VGC27_08590"
     CDS             complement(2274..2891)
                     /locus_tag="VGC27_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282666.1"
                     /translation="MADTSFVGESAATLAASLMRLRTDEETRRRALRRGLAAAAVVVL
                     HAAFFYMLLQADWLPAMRTIPPAKAPLLWLLLPKTTGTPKAVQQQAQKEGAPDRPVMR
                     NLPITLPPRPDAIDFGLVLGRALACGANSFEYLTPEGRSSCKRTPWNYTYAKDGTIIL
                     NTQYQRPEPQPTPADVLAHERYTAPACPEYIDPNAPCISGIIGGH"
     gene            3117..3551
                     /locus_tag="VGC27_08595"
     CDS             3117..3551
                     /locus_tag="VGC27_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282667.1"
                     /translation="MRATIIAAMSFAASLGLAGCDNSANVPAPTVQAVAPPCNCEPQN
                     EAVTKTVAPRAKHRHARTASYREYEEDRSARSESRSTYQGGSGEQSDYQTAYQEYGHS
                     EEAVWVDGYGRSHFVAAASAAPDDRARLAPWHGYDEDCDDKK"
     gene            complement(3675..5111)
                     /locus_tag="VGC27_08600"
     CDS             complement(3675..5111)
                     /locus_tag="VGC27_08600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF006606.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY0282668.1"
                     /translation="MEQEAYRHVPYDIEVEQALLGAILVDNRVLETVSPLIRSDHFYD
                     PLHQRLYDAMAASFERGGMVLTPLTLHAAMKADPGLQEVGGLAYLAGLAGAAPAMPNA
                     RDLARILHDLAVRRALIHIGEDIVNTAYEAPHEKPPQAQIEEAEKALYRVSETSKYGE
                     GPLDFSESLRRAVETAERAQARGGKISGVSSGFSDIDSLLGGLQPSDLLIIAGRPGMG
                     KTALGTNMAFHAARAWQQDTEAGVEVPNGAPVLFFSLEMAAQQLSARILSEQTEIEMW
                     KIRNGRFSESEWEKFVLTMQDLSTLPLYIDDTGGISIAQIAARARRMKREKKIGLVII
                     DYLQLVDPSRRAENRVQEITEVTKGLKALAKELNVPVVALSQLSRGVDSRDDKRPVLS
                     DLRESGSIEQDADVVMFVYREEYYLKSREPEIGTNEHTKWLEKCERAHRKAEVLVEKH
                     RHGATNKVDLYFDDRFTRFSNLANEGNR"
     gene            complement(5241..6485)
                     /locus_tag="VGC27_08605"
     CDS             complement(5241..6485)
                     /locus_tag="VGC27_08605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY0282669.1"
                     /translation="MFRRLLVSLIRQGTLTVVRPNGRRESFGSGEPRVAVRLHDRRAI
                     FELVLNPDLKLGELYMDGRLTLEQGDVADLLGLLMMNLRTARPPALYRLGAAFRFLTR
                     RFAQFNPAWRAKAHAAHHYDLSGRLYDLFLDRDKQYSCAYFTAPGETLEEAQIGKKRH
                     IAAKLNLDRHGLKVLDIGCGWGGLALDLARDLGASVLGVTLSENQVAVARERSREARL
                     AERCKFELVDYRALGGTYDRIVSVGMFEHVGVAYYPAFFAKVRDLLAEDGTILLHTIG
                     RSDGPGSTNPWIAKYIFPGGYIPALSEMMQAVEKSGLIITDVEILRLHYAETLKEWRS
                     RFLANRAEAARLYDERFCRMWEFYLAGSEMAFRHDHQVVFQIQLTKRQSALPLTRDYM
                     VDGERTMRFAGTEQMPSARRAG"
     gene            complement(6784..7272)
                     /locus_tag="VGC27_08610"
     CDS             complement(6784..7272)
                     /locus_tag="VGC27_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282670.1"
                     /translation="MPGEEQSSSLRDEELRRLAAERLAEHLDIGASLIARCEHLAELP
                     KGDRLGAIYAAARLMHANAHVARAFAQVAQLERRQRTIIEHIQPLVLKSADSNSSLEN
                     ELERELRLKMLRYMKLVAAETLDPALDEAAAAAPEDNAFQNDAPQKTPLASALGSSAG
                     PA"
BASE COUNT         1525 a         2473 c         2544 g         1446 t
ORIGIN      
        1 atgctgtcga agagcgcggc gccctggaac agcatgccga atttcttcag caccgtgtcg
       61 cgcgagcggc cgcgaaggcg cgtaacgtcc ttgccgtcga cgaagatctc gccggcgtcc
      121 ggcctgagga tgccgagcac gcatttgatc atcacggatt tgccggtgcc ggaaccgccg
      181 atcaccacca gcgattggcc gggctggatc gtaaggtcga cgccgtcgag tacgatcttg
      241 cggccgaagc gcttcttgac gcctctcaac tccacctttg ccgtgctgcc tgcgctcgtc
      301 ataacggtca cttctagaac aacaggttgg tgagaaggta gttggcggcg agaatcaaga
      361 tagatgccga caccaccgcg ttggtcgtcg ccgcgccgac gccttgcgcc ccgcccttgg
      421 aatggaagcc gttaaagcag cccatcagcg cgatgatgaa gccgaaggcg gccgccttga
      481 tgaggccgga agagacatcg tggagggtca tggcatcgac cgtattcttg agatagatcg
      541 agcccgtgaa accaaggctt tgtgtcgata cgatataacc gccgagaata ccgatggaat
      601 cggcgacagc caccagaatc ggcatcgaga ccaccgccgc cgccagcctc ggcacgacga
      661 gatacttgat gggattggtg gccagcgtgg tcagcgcgtc gatctgttcg gtcacccgca
      721 tggtgccaag ttcggcggcg atggcggcgg cgacgcgtcc ggccaccatc aggccggcga
      781 tcaccggccc caactcgcgc gtgatgccga ccatcacaac caccggcacg aaggcttccg
      841 cgccgtaacg ggcggcgccg gtataaatct gcaaagccag cgcgccgccg gtgaagaacg
      901 ccgtgaggcc cacgaccggc agcgaaaaat agccgatacg catcatctgc ttgagaacca
      961 gcgaccaata gatcggcggg cggaaacacg cggtgacggc gtccagggtg aagacggcca
     1021 ggcgtccgat ctcacccagc agagccaagc tcacccgtcc gatggcggca aagacgttca
     1081 tggcgcagcc tcgtaaaccc gcgtcgcgcg gtgtgaaagc gacgtcagga tttcatagga
     1141 tatcgtgttc gcagcgacgg caatctcctc gagcgggatc gtgtcgccga agaattccac
     1201 ctccatgccg gcggcgagcg aacccgttac atcggtcacg tccagggtca ccagatccat
     1261 ggagacgcgc ccgacgacag gcacacggac gccgccaacg gccccgctac cgcgactgga
     1321 caccgcgcgc atcagcccat cggcgtaacc tacgcccgcc gttgccagca gcgacggccg
     1381 cctcgtccgg aaggtggcgc cgtagccgac ggtctcgccc ttgtcggcgc ggtgaagctg
     1441 gaggatgcgc gcggtcatcc gtaccgccac ctggaacgga tttggctttg ccggctgcgg
     1501 attgccgccg taaaggcaga ggccggggcg caccatgtcg aaaagaaact cccggccgag
     1561 cagaatgccg ccggatgcag ccaagcttgc cggcgccggg ggcagcatcg ccagagcggt
     1621 cttgaagcgg ccaagctgga tgcggttcat cttggcttca ctgtcgtccg agcaggccag
     1681 atggctcatc accaggacga gccggaggcc gtccaggcgt ttggcgtgat cggcggcaag
     1741 cgtcgccagt tctgcaccgg gaagacccag ccggttcatg ccggtgtcga tgtgaatcgc
     1801 cgcatcgagc cgcgtccggg tggcgcgcgc ggccgccgac cagccggcaa tttcggcgat
     1861 cgaattcagc accggggtga ggcgatggct gatgagggcc ggcactgcat cgggctgtgc
     1921 tccgtccagg acaaaaatcc gcgcttccgg cgccagcgga cgcaaggcaa gcccctcttc
     1981 cagccttgca acaaagaacg tgtcgcaacc ggcgtggacc aggaccgatg cgactggact
     2041 aagcccggta ccataggcgt cggccttcac cgccgccgcc gccgtggccg tgccggcaag
     2101 gcgctgggcg gtcttgaaat tggcggcgat ggcaccgaga cgcaccacca agcgcgccgc
     2161 ttcgaagtct gcgattctgg gttcgtccgt gcggtccatg ggagtcgaac aatgcagccc
     2221 tgcccgcctc gcgtcaaagc ctgtcctgta atgacgctgc ggcgtgctgt tcatcaatgc
     2281 ccccctataa tgccggaaat gcagggtgcg ttcggatcga tatattccgg gcaggccggg
     2341 gccgtgtacc gctcatgcgc caggacgtcg gccggcgtgg gctggggttc cgggcgctga
     2401 tattgcgtgt tcaagatgat cgtgccgtcc ttggcataag tatagttcca gggtgtgcgc
     2461 ttgcagctcg accgcccctc cggcgtgaga tattcgaaac tgttcgctcc gcaagccagc
     2521 gcccggccga gcaccagccc aaagtctatg gcatcgggcc gcggcggcag ggtgatcggc
     2581 agattgcgca ttacgggcct atcaggggcg ccttccttct gtgcttgctg ctgcacggct
     2641 ttgggcgtgc ccgtggtctt cggcaacagc agccacaaca gaggagcctt ggcaggcggt
     2701 atcgtgcgca tggcgggaag ccaatcggcc tgaagcaaca tgtagaagaa cgctgcatgc
     2761 agcacgacca cggcggccgc cgccaagccg cgccgcaggg cacgcctgcg cgtctcctca
     2821 tccgtcctga gacgcatcag ggacgcagcc agcgtggcag ccgactcacc gacgaaagac
     2881 gtgtctgcca ccgaaatgcc tattcaccat gggaccggcc gacgaacgtg ggtgcatagg
     2941 cagtttatgc catctgcagc atggtggcga ctatagcatt gtacgtaatg aacattccca
     3001 gccgttggaa gattctccgg tcgcggttaa gcatgcaaag cagctctgct tgccacaccc
     3061 gggacggcgc gggaagcttg ccgttccggg gttgtttgcc ggtagggatg actgaaatgc
     3121 gtgcgacgat tatcgctgcg atgagctttg cggccagcct gggtttggcc ggttgcgaca
     3181 attccgccaa tgtgccggcg ccgaccgtgc aggccgtggc gccgccttgc aattgcgagc
     3241 cgcagaacga ggctgtcacc aaaaccgttg caccccgcgc aaagcatcgt cacgcccgga
     3301 cagcatctta ccgcgaatat gaagaggacc ggtccgctcg cagtgaatcg cggagcacct
     3361 accaaggcgg gtccggcgaa cagagcgact atcaaaccgc gtatcaagag tatggccact
     3421 cggaagaagc cgtctgggtc gacggctatg gccgcagcca tttcgtcgcg gctgcatcgg
     3481 cggcacccga cgatcgcgcc cgcctcgcgc cgtggcacgg ctacgacgaa gactgcgacg
     3541 acaagaaata gaagctagag cgcaatccgg caaagtggtc ggtagcagca gcgtgccgac
     3601 gcggtttgcc gtcagattgc gcgaaaaaca aggaagctag agcttgatca gattcaatta
     3661 aatcggatca agctctagcg gttgccttcg ttggccagat tgctgaagcg cgtaaagcgg
     3721 tcgtcgaagt aaagatcgac cttgttggtg gcgccgtggc ggtgcttttc gaccagcacc
     3781 tcggccttgc ggtgggcgcg ctcgcacttt tccagccact tcgtatgttc gttggtaccg
     3841 atctccggct cgcgcgattt caggtagtac tcttcgcgat agacgaacat gacgacgtcg
     3901 gcgtcctgct cgatggaacc ggattcgcgc aggtccgaca gcaccgggcg cttatcgtcg
     3961 cgagaatcca cgccgcgcga aagctgcgac agcgccacca ccggcacgtt cagttccttg
     4021 gccagcgctt tcaggccctt ggtcacctcg gtgatttcct gcacccggtt ctcggcccgc
     4081 cgcgaagggt ccaccaattg cagataatcg atgatgacga gaccgatctt cttctcgcgc
     4141 ttcatgcgcc gcgcccgcgc cgcgatctgg gcgatggaga tgccgccggt gtcgtcgata
     4201 tagagcggca gggtggatag gtcctgcatg gtgaggacga acttctccca ctcgctttcc
     4261 gagaagcggc cgttgcggat cttccacatc tcgatctcgg tctgctcgga gagaatgcgc
     4321 gcggatagct gctgcgccgc catttccagc gagaagaaca gcaccggcgc gccgttcggc
     4381 acctccaccc ccgcctcggt atcctgttgc caggcgcggg cggcgtggaa cgccatatta
     4441 gtgccgagcg ccgttttgcc catgccggga cggccggcga tgatcagaag gtcggagggt
     4501 tgcaaaccgc cgagcagact gtcgatgtcg gaaaagcccg acgacacgcc ggaaatcttg
     4561 ccgccgcgcg cctgggcgcg ctcggccgtc tccacggcgc ggcgcaggct ttcggagaaa
     4621 tcgagcggac cttcgccgta tttcgaggtc tcgctgacgc ggtagagcgc cttttccgcc
     4681 tcctcgatct gggcctgcgg cggcttttcg tggggcgcct cgtaagcggt attgacgata
     4741 tcttcgccga tatggatcag cgcgcgccgc accgccagat cgtgcaggat acgggctaga
     4801 tcgcgcgcgt tcggcatcgc cggcgcggct ccggccaggc ccgccagata agcaagaccg
     4861 ccgacctctt gcagccccgg atcggccttc atcgcggcgt gcagcgtcag cggcgtcagc
     4921 accatgccgc cgcgctcgaa ggacgccgcc atcgcgtcgt agaggcgctg atgcaacgga
     4981 tcgtagaaat ggtctgaccg gatcagcggg gaaacggttt ccagcacgcg attgtccacc
     5041 aggatcgcgc cgagcaaagc ctgctcgacc tcgatgtcgt aggggacgtg gcggtaggct
     5101 tcctgttcca tggcgctttt tcgggcttat ccgggcggct tcggtcgcga aggcagacag
     5161 cgaatcaagc gacgcagacc ccttgccgca agtgcaattc cgattcgatg cacagctaaa
     5221 tcgcgcggcg cacagcccgc ttagcccgcg cggcgcgcgc taggcatttg ttcggttccc
     5281 gcaaagcgca tcgtgcgttc gccgtccacc atatagtcgc gcgtcagcgg cagcgcgctt
     5341 tggcgcttgg tcaactgaat ctggaacacg acctgatggt cgtggcggaa cgccatctcc
     5401 gagccggcga ggtagaactc ccacatacgg cagaagcgct cgtcgtagag gcgcgcggcc
     5461 tcggcgcggt tggcgaggaa gcggctgcgc cactccttca gcgtctcggc gtagtgcagg
     5521 cgcagaatct ccacgtcggt gatgatgagc ccgctctttt cgacggcctg catcatttcg
     5581 gatagcgccg ggatataacc gccggggaaa atatacttcg cgatccaggg attggtggag
     5641 ccggggccgt cgctgcggcc gatggtgtgc agcagtatcg tgccgtcttc ggctagaaga
     5701 tcgcgcactt tggcgaagaa ggccgggtaa taggccacgc ccacatgctc gaacatgccg
     5761 acggagacga tgcggtcgta ggttccgccc agggcgcgat aatccaccag ctcgaacttg
     5821 cagcgctccg ccagccgcgc ctcccgcgaa cgctcgcgcg ccaccgcgac ctggttctcg
     5881 gacagggtga caccgaggac gctggcgccc aagtcgcgcg ccagatcgag cgccaacccg
     5941 ccccagccgc agccgatgtc cagcactttc aatccgtgcc ggtcgagatt gagcttggcc
     6001 gcgatgtggc gcttcttgcc gatctgcgct tcttcgagcg tctcgcccgg cgcggtgaaa
     6061 taagcgcagg aatattgctt gtcgcggtcg aggaacaaat catagagccg gccggagaga
     6121 tcgtagtgat gcgccgcatg cgccttggcg cgccaagcgg gattgaattg cgcaaaacgc
     6181 ctggtcaaga agcggaacgc cgctcccaac cggtacaggg cgggcgggcg cgcagtccgc
     6241 agattcatca tcagcaaccc gagcaggtcg gcgacgtcgc cctgctcaag cgtaagccgt
     6301 ccgtccatgt aaagttcgcc gagcttgaga tcgggattga gaaccagctc gaaaatggcg
     6361 cggcggtcgt gaagcctgac cgcgacacgc ggctcgcccg aaccaaaact ctcccgccgc
     6421 ccgttgggac ggaccacggt cagcgtacct tggcggataa gagagactag aagacgacga
     6481 aacattcgac ctctccctag aaggcggcca gcctgccggc cgacgtgatg ataaacgctc
     6541 tactccccga taggttttct gcgaggtcag tttacctcat ttgctccctt ccgttccact
     6601 gccgcgtccg aaatcttggc cttacggcgc cgcctgcccg tcgcctggaa gcagggaaac
     6661 ccggccgcga gcaaagcgcg gccgcgcctg atatgcgcgc ggacctcggc cagcagcgcg
     6721 cggcgctcgc gcgtatactt gccgtgcctg agcgcgttgc tgtttcccgg ctggccgccg
     6781 cgcttaagcg ggaccggcgg aggagccgag ggcggacgca agtggcgttt tttggggcgc
     6841 atcgttttgg aacgcgttgt cttcgggtgc agcggcggcg gcttcatcaa gcgcggggtc
     6901 gagggtttca gccgcaacga gcttcatgta gcggagcatc ttcaaccgaa gttcgcgctc
     6961 cagctcattt tccaaacttg aattcgaatc ggccgatttt aggacaagcg gttgaatgtg
     7021 ttcgatgatc gtcctctgac ggcgctccaa ctgagcgacc tgcgcgaatg cccgcgcgac
     7081 gtgtgcgttg gcgtgcatca agcgggcggc ggcgtagatg gcgccgagcc ggtcgccctt
     7141 gggcaactcc gccagatgct cgcagcgcgc aataaggctc gcgccgatat cgagatgttc
     7201 ggcaaggcgc tcggccgcga ggcggcgaag ctcctcgtcg cgaagggacg acgattgttc
     7261 ctcgccgggc acaacagcac tcgtttggcc gccgctgcgg gcggcctggg ggtcaacctg
     7321 ggtcatggta atgatataga gaacgaaagg tgaacttcaa gagggaagag agaaattatt
     7381 ttcatcctgc gccgccccct ttgtattacg ggaaaacgca ggatgttccg tattgaccat
     7441 ctcggggcct gcttgcttga tcaaagcgcg cagaagcatc ttgccccgtc tgctcccctt
     7501 cgcggcatag ccgctcgaag atggcgcgcc gagtcggatc ggcgagcgtc ttgaagagca
     7561 cttcgcgggt gtgtgccatc gggacgtata acttgttagt tattgattag atcaataacc
     7621 cacgagttat gtgagagcca atcgagaatt tcgggaagag cgtgatccga ttcaattaaa
     7681 tcggatcacg ctctaacgca tttcatcgtt tcacggaacc gatgaaatga gctggattct
     7741 tagtttgtag cgttttcgga cggaaaaccg cccggcgcgc tttgcgcgcc tattggttcc
     7801 atagcgcgct ttcgcgcggg cacttttcct gaaaacactc tagcaatcca tccgtcaaaa
     7861 ctggatgttg gttctgacgc caatgacaaa ggcgtttttc atcgccggag cgggcgcgga
     7921 cgggtcggct gtgggatcgg cggcatatcc ggcgggatgg atgatgtatt ggatgtccgg
     7981 ctggatga
//
LOCUS       DASWPU010000398         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_83088,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000398 DASWPU010000000
VERSION     DASWPU010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83088"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..>1738
                     /locus_tag="VGC27_08615"
     CDS             164..>1738
                     /locus_tag="VGC27_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0282671.1"
                     /translation="MPDMKILPPVSVEARRLLPSPWDLLAAVLVLGFIVLLADASRAM
                     IQPLSSLVQQPISLDPAALPGYALRTGLRMLIALAISLLFTFTYATWAAKSARAGTLL
                     VPLLDILQSVPILGFISVTFVFFLSLAPGRILGAEFAAIFAIFTSQAWNMAFSFYQSL
                     RTVPEELEEAGRLFGLNAWARFWRIEVPYGMPQLVWNMMMSMSGAWFFVVAAEAFTVG
                     NTSIALPGIGSYIATAIADKNMSAILWAIMAMLFVILIYDQLLFRPLVAWADRFRIDA
                     EGGDEAAESWALKMFRRSRLMDAIAVPFDALMHWTYRRTAPSRGRSANGSGILPSRLA
                     DAVWYACLLALVLYVLWRVVSFAGIPLGGGEFLNICLRGLATLTRVVVLIALASAVWT
                     PVGIYVGLRPQLTRIVQPVAQFLAAFPANLLFPVAVSLIVMWNLDPNIWLSPLMVLGT
                     QWYILFNVIAGASALPRELRDASNNLQLKGWLWWRKVALPAVFPYYLTGAITASGGSW
                     NAAIVAEIVVWGHKTLR"
BASE COUNT          233 a          597 c          552 g          356 t
ORIGIN      
        1 cacgcgccac aatgcgccgc gcgtcaaagc gacagcggcg gcagagaaaa gaacaaatcc
       61 ttcctttttt cacttggccc gcagcggcga gacgtggttg tgtgcggccg agccgcgccg
      121 tctcgcggac tgcccgccaa cactagatca atcgcttcga ggcatgccgg atatgaagat
      181 cctgccgccc gtctcggtcg aggctcgccg cttgctgccc agcccgtggg acctgctcgc
      241 ggccgtgctg gtgctcggct tcatcgtgct gctggcggac gccagccgcg ccatgatcca
      301 gccgctgtcg agcctggttc aacagccgat ctcgctcgat cccgccgccc tgcccggcta
      361 tgcgctgcga acgggcctgc gcatgctgat cgcgctggcg atttcgctgc tgttcacctt
      421 cacctacgcg acctgggcgg ccaagagcgc gcgcgccggt acgcttctgg tgccgctgct
      481 ggacatcctc cagtcggtgc ccatcctggg cttcatctcg gtgaccttcg tgttcttcct
      541 gtccctggcg ccggggcgga ttctgggcgc ggaattcgcg gcgattttcg ccattttcac
      601 cagccaggcc tggaacatgg ccttcagctt ctaccaatcg ttgcgcacgg ttccggagga
      661 actggaggag gcgggacgtc tgttcggcct caatgcctgg gcgcgcttct ggcggatcga
      721 ggtgccctac gggatgccgc agcttgtctg gaacatgatg atgtcgatgt ccggcgcctg
      781 gttcttcgtg gtggccgccg aggccttcac cgtcggtaat acctccatcg cccttcccgg
      841 catcggctcc tacatcgcca ccgccatcgc ggacaagaac atgtccgcga tcctctgggc
      901 catcatggcc atgctgttcg tcatcctgat ctacgaccag ctcctgttcc gcccgctggt
      961 cgcctgggcc gaccgtttcc gcatcgacgc cgagggcggc gacgaagcag ccgaatcctg
     1021 ggccttgaag atgttccgcc gctcgagact gatggacgcc atcgccgtgc ccttcgacgc
     1081 gctgatgcac tggacctacc gccggacggc gccctcgcgg ggccggagcg cgaacggctc
     1141 gggtatcctg ccgtcgcggc tcgccgatgc cgtctggtac gcctgtctgc ttgccttggt
     1201 gctgtacgtg ctgtggcgcg tggtcagttt cgccggcatc ccgctcggcg gcggggaatt
     1261 tctcaacatc tgcctgcgcg gcctcgccac gctgacccgc gtcgttgtgc tgatcgcgct
     1321 ggcgagcgcc gtctggacgc cggtcggcat ctatgtcggg ctgcgcccgc agctcacgcg
     1381 gatcgtccag ccggtggcgc agttcctggc cgcgttcccg gccaatctgc tgttcccggt
     1441 cgccgtctcc ttgatcgtga tgtggaacct ggaccccaat atctggctgt cgccgctgat
     1501 ggtgctgggc acccaatggt acatcctgtt caacgtcatc gccggggctt cggcgctgcc
     1561 gcgggagctg cgcgacgcca gcaacaatct tcagctcaag ggctggctgt ggtggcgcaa
     1621 agtggcgctg cctgcggtgt ttccctacta cctcacgggc gcgatcacgg cctcgggcgg
     1681 ttcgtggaac gcggccatcg tcgcggaaat cgtggtgtgg ggccacaaga ccctgcgc
//
LOCUS       DASWPU010000399         4063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_88818,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000399 DASWPU010000000
VERSION     DASWPU010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88818"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VGC27_08620"
     CDS             <1..282
                     /locus_tag="VGC27_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282672.1"
                     /translation="DIDLETDRGALSLEAYQGTLVADGKQIAAGTAEAKYPLLYQRFA
                     ELIQQHESDVDKRPLQLVADAFLVGRRIPVEPFDVPRQELALDGAVLGR"
     gene            436..1584
                     /locus_tag="VGC27_08625"
     CDS             436..1584
                     /locus_tag="VGC27_08625"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEY0282673.1"
                     /translation="MIDVARAGVAGLTFAVAAMIASASAKDAAAMPQKAPFGHTADGK
                     AVDIYTLNNKNGVEARIMTYGGTRVSLKVPDRDGHLGNVVLGFDTLEPYLAGVPYYGA
                     TIGRFGNRIAGGSFTLDGVAYQLPKNDGPNSLHGGTVGFDKRVWTAKPFVNTQGPGLK
                     LTYVSADGEEGYPGQLTTHVSYQLRNDNSLSIDYRATTTKPTVVNLTNHSYFNLSGDP
                     DHSIVDEILTINADTFTPVSATLIPTGVLEPVAGTPFDFRKPTVIGAHINDDNQQIHY
                     GPGYDHNWVLVKPKPGAMTTAAMVSDPVSGRTLEVKTTEPGLQFYTGNFQDGKPAGTG
                     TVFKYRTGFTMETQHFPDSPNQPSFPSTVLRPGHTFVSHTVFIFRTAK"
     gene            complement(1787..3022)
                     /locus_tag="VGC27_08630"
     CDS             complement(1787..3022)
                     /locus_tag="VGC27_08630"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEY0282674.1"
                     /translation="MGWNSWDSFGPTIREDEARANAAIMAGRLLPHGYTIFTVDIQWY
                     EPGANGYDYRKGAELTMDAWGRLLPAPNRFPSAAGGAGFKPLADHVHGLGLKFGLHMM
                     RGVPRQAADRDTPILGTDIRAGEIADRVNVCPWNTDMYGIDMSKPGAQAYYDSVFRLY
                     ASWGVDFVKADDLSRPYIRNKPEIEAVRAAIDRSGRPMILSLSPGETPLAAAAHVRSH
                     ANMWRISDDFWDTWPALLEQFTRLADWNATRRPGNWPDADMLPLGLLEMGKRRTRFTP
                     DEQKTLLTLWSIARSPLIMGGDLRHLDEATYALLTNDEVLGVNQVSRNNRQIVRSDEM
                     AVWAAQDARGSETIVALFNLRDPGPDAPVPVTLAQLGLTGTVRVRDLWQRRAIAAAQG
                     RFAPGIPCHGAGLYRLTRA"
     gene            complement(3142..>4063)
                     /locus_tag="VGC27_08635"
     CDS             complement(3142..>4063)
                     /locus_tag="VGC27_08635"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0282675.1"
                     /translation="VFKQYATFVRTQRGARPIVIASGGHDEDLTFTQALMSAVKWGMD
                     AISFHYYTIPSPTHNWEAKGPSTGFGEDQWISTLANTLQMDTYLKKNIAIMNKYDPKK
                     RVGFYVDEWGTWYDPEPGREPGFLYQQNTVRDAVVAAVNFNLFHKYADRVRMTNIAQT
                     INVLQAMILTDGPKMVLTPTYHVFHMFIPFQDATLLPTDLTTPRYQLGKASVPAVSVS
                     AARGKDGKLEYALVNLDPDRPAHVSTTIAGESVRHVGGTILTGSAMDARNTFDDPDAV
                     HPVPFTGAELAGNRLEVTLPPKSVVVLSLD"
BASE COUNT          796 a         1316 c         1221 g          730 t
ORIGIN      
        1 gatatcgacc tcgaaaccga tcgcggggcg ttaagcctgg aagcatacca gggcacgctg
       61 gtcgccgacg gaaagcaaat cgccgccgga acggcagagg cgaagtatcc gctgctttac
      121 caacgctttg ccgaattgat ccaacaacac gaatccgacg tcgacaagcg gccgcttcaa
      181 ctggtggccg acgcctttct tgtcggcagg cgaattccgg tggaaccctt cgacgttccc
      241 cggcaggaac tggcgctcga tggagcggtt ctcggccgtt gagccgccgg ggcgtgggcg
      301 gcttgacgga aatcttgctt tttccgccaa aaaagcctcc gtctcgattt caatttcgaa
      361 ttgtctgatt taatatccgc cctcgcagcg gcgtcaggca cagcggccgc agaccaacag
      421 gaggaatttg acgccatgat cgatgttgcg cgcgccggcg ttgccgggct gacgtttgcg
      481 gtagcggcga tgatcgcctc ggcgagcgcg aaagacgccg ccgcgatgcc ccagaaggcg
      541 ccgttcggac acacggcgga cggcaaagcc gtcgatatct atacgctcaa caacaaaaac
      601 ggcgtcgagg cgcgcatcat gacctatggc gggacgcggg tttcgctgaa ggttcccgac
      661 cgggatggcc atctgggcaa cgtcgtgctc ggtttcgaca cgctcgaacc ctatctggcc
      721 ggcgtgccct attacggagc gaccatcggg cgcttcggca accgcatcgc cggcggcagc
      781 ttcaccctgg acggcgtcgc ttaccaactg cccaagaacg acggccccaa cagcctgcac
      841 ggcggcaccg taggattcga caaacgagtc tggacggcga aacccttcgt gaacacgcag
      901 ggccccggcc tcaagctcac ctatgtcagc gccgacggcg aggagggtta tcccggccag
      961 ctcacgaccc atgtcagcta tcagctccgc aacgacaatt ccctgagcat cgattaccgg
     1021 gcgaccacca ccaagccgac ggtcgtcaac ctcacgaacc acagctattt caatctttcc
     1081 ggcgacccgg atcactcgat cgtcgacgaa atcttgacga tcaacgccga cacgttcacg
     1141 ccggtgagtg cgacgctgat cccgaccggc gtgctggaac ccgttgccgg cacgccgttc
     1201 gatttccgca agccgaccgt catcggcgcg cacatcaacg acgacaacca gcagatccac
     1261 tacggccccg gctacgacca taattgggta ctggtgaaac cgaagcccgg tgcgatgacg
     1321 acggccgcca tggtcagcga tccggtcagc gggcgcacac tcgaagtgaa gacgaccgaa
     1381 ccgggactac agttctacac cggtaatttc caagacggta agccggccgg aacgggcacg
     1441 gtcttcaagt accgcaccgg cttcaccatg gagacgcaac attttccgga ctcgcccaac
     1501 cagccgtcct tcccctcgac ggtcctgcgc cctggccaca ccttcgtttc acacacggtc
     1561 ttcatcttcc gcacggcgaa ataggcccgg aggcaccgaa aaaacgacag cagcggcggc
     1621 cccccgccgc tgctgtcgtt ctcggagaag acgctagagc ttcgtacgtt cagacgagat
     1681 cacgttgtct tcatgcttcg acaagctcag catgagggat tggaaaacgc ctttaccctg
     1741 agcttgtcga agggtgtggg atgactccgt ctgagtgcaa tttgctctag gcgcgcgtca
     1801 accggtagag ccccgcacca tggcacggga tgcccggcgc aaaccggcct tgcgcggccg
     1861 caatcgcgcg ccgctgccaa aggtcgcgca cccgcaccgt gcccgtcagt cccaattgcg
     1921 caagcgtgac cggcacgggc gcatccggcc cagggtcgcg cagattgaac agagcgacaa
     1981 tcgtttcgct gccgcgcgca tcctgcgccg cccaaaccgc catctcgtcg ctgcggacga
     2041 tctgccggtt gttccggctc acttggttga cgccaagcac ctcgtcattg gtcagcaagg
     2101 cataggtcgc ttcatcgaga tgcctcagat cgccgcccat gatcaaaggc gagcgcgcaa
     2161 tcgaccatag ggtgagcaaa gtcttctgtt catcgggggt gaaccgggtg cggcgcttgc
     2221 ccatctcaag caggcccagc ggcagcatgt ccgcatcggg ccaattgccc ggccggcggg
     2281 tggcattcca atcggcgagc ctggtaaatt gttcgagcaa ggcgggccaa gtgtcccaga
     2341 aatcgtcgct gatgcgccac atattggcgt gactccgcac atgcgcggcc gccgccagcg
     2401 gcgtttcccc gggcgagagc gagaggatca tcgggcggcc gcttcggtcg atggccgcac
     2461 ggaccgcttc gatctccggc ttgttgcgga tatagggacg gctcaggtca tcggccttga
     2521 cgaaatcgac gccccaggac gcatacaggc ggaagacgga gtcgtaataa gcctgcgcgc
     2581 cgggcttcga catgtcgatg ccatacatat cggtgttcca ggggcagaca ttcacccggt
     2641 cggcgatttc gccggcgcgg atatccgttc ccaggatcgg cgtgtccctg tcggccgctt
     2701 ggcgcggcac gccgcgcatc atatgcaaac cgaatttgag gccgaggcca tgcacatggt
     2761 ccgccaacgg tttgaacccc gccccgcccg ccgcggaggg aaagcggttc ggcgcgggca
     2821 acaacctgcc ccacgcgtcc atcgttagtt cggcgccttt gcggtagtcg tagccgttcg
     2881 cgcctggctc ataccattgg atgtcaacgg tgaagatggt gtagccgtgc ggcagcaaac
     2941 gcccagccat gatcgccgcg ttggcgcggg cttcatcctc gcggatcgtc ggcccgaaac
     3001 tgtcccacga attccagccc atgggcggca tcggcgccag cgagcgaaaa tccggcgctt
     3061 gagcgccgct atatgcaatg gcggggctca caacgagccc cgccacgaca ttccgccggg
     3121 tcagacctcg acccatttcc gctagtcgag gctcaacacg acgaccgatt tcggcggaag
     3181 cgtcacttcc aaccggttgc cggccagttc agcgccggta aacggcaccg gatgcacggc
     3241 gtcgggatcg tcgaaggtgt tgcgcgcatc catcgccgaa ccggtcagga tggttccgcc
     3301 gacgtgccgc acgctttcgc ctgcaatcgt cgtggaaaca tgcgccggac gatcggggtc
     3361 gagattgacc aaggcgtatt ccagcttgcc gtccttgccg cgcgccgccg agacgctgac
     3421 cgccggcacg gaggccttgc ccaactggta acgcggcgtc gtcaagtccg tcggcagcaa
     3481 cgtcgcgtcc tggaacggga tgaacatgtg gaagacgtga taggtcggcg tcagcaccat
     3541 cttcgggccg tcggtcagga tcatcgcctg caacacattg atggtctgcg cgatgttggt
     3601 catgcgcacg cggtcggcat atttgtggaa aaggttgaag ttcaccgccg cgaccacggc
     3661 atcgcgcacc gtattctgct gatagaggaa gcccggttcc ctgcccggct ccggatcgta
     3721 ccaggtgccc cattcgtcga cgtagaaacc gacccgcttc ttcggatcgt atttgttcat
     3781 gatggcgatg ttcttcttga gataagtatc catctgcagc gtattcgcca gcgtggatat
     3841 ccactgatcc tcgccgaagc cggtcgaggg ccccttcgcc tcccagttat gggtgggcga
     3901 cggtatcgtg taatagtgga acgaaatcgc atccatgccc catttgacgg ccgacatcag
     3961 cgcctgggtg aaggtcaaat cctcgtcatg gccgccgctg gcgatcacga tcgggcgggc
     4021 gccgcgctgc gtcctgacga aggtcgcgta ttgcttgaag acg
//
LOCUS       DASWPU010000400         3440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_113815,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000400 DASWPU010000000
VERSION     DASWPU010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113815"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2146
                     /locus_tag="VGC27_08640"
     CDS             <1..2146
                     /locus_tag="VGC27_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0282676.1"
                     /translation="IPTSFLMGFMVLNGVGYTLNFMIMFALLLAVGILVDGAIIVVEY
                     ADRKMTEGHPPREAFAEAARRMFWPVVSATATMIGAFVPMLLWPGIIGSYMSFFPITL
                     IIVLSSSMVVALIFLPVLGGWFGRPPPRDEEHQRSIEASETGDWREISGITGWYARLS
                     EKLSQRPGRVLLGAFGIVVAVIAFAAVFFKGVEFFVTTDPEMANIYVSARGNLSAAEK
                     RDLVMSVERRIEDVEGLQYLYAKTGGGGQRAPVDVIGRIVVEMKPKAERARSGKAILE
                     EVRKRTEGMAGIRVEVTEPQSGPSGGKDIVVDVTSEDYVSLLRVVDALRRHMDSQPEL
                     RDVEDTRALPGIEWDLKINRELASRFGVNTLMIGTAVQLVTNGIFVARYRPDDSEDEV
                     DIRVRYPSAWRGVHALDNLRIATGDGSVVPLSNFVTVVPSQLTNQIEHADGRRVYHVR
                     ANMKPNTLLPNAEMAKLKTWIEAQNFPTTVHTAFKGSTEDQDESMAFLGKAALMAVFL
                     IAIVLLAMFNSVYHTLLILVAVVLAMIGAVLGMVVMGQTFSVVMTGTGLMALAGVVVN
                     HNIVLIDTFHRHIKSGMDPIEAVIRSSAQRLRPVFLTTVTAIGGLLPMMFAVEINFWH
                     RTIEVGSANASMWVQLSTAVIFGLAFSKAITLGLVPALLALPYRVRERHGSFLAMFAT
                     LPGRLGRALLRPWRSLRRRLGLAAQPDAAPAE"
     gene            complement(2147..>3440)
                     /locus_tag="VGC27_08645"
     CDS             complement(2147..>3440)
                     /locus_tag="VGC27_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY0282677.1"
                     /translation="FIITRWRIQFRRVMNESDTEANTKTVDSLLNYETVKYFNNETHE
                     TRRFDVSMKKYADASVQTQISLSVLNTGQAVIVALGLAVIMGLAAVAVAHGRFSVGDL
                     VMANSYLIQLYVPLNLLGTVYREITQALVDMDAMFHLLHQPQEVKDKPDAKPLAISGG
                     EIRFDNVVFAYDPDRTVLKGVSFTVPAGKTIAVVGPSGAGKSTISRILFRFYDIKGGS
                     VTIDGQDIRDVTQASLRAAIGIVPQDTVLFNDTVRYNIAYGRIGASEAEIKEAARHAQ
                     IDKFIGDLPLGYESMVGERGLKLSGGEKQRVAIARTILKNPPILLLDEATSALDTHTE
                     REIQGALQEISKNRTTLVIAHRLSTVVDADEILVLDHGEIVERGRHDDLIARKGAYAS
                     MWNKQKKAAAARERIKKIEADPQVTPGVARIVPLAAAD"
BASE COUNT          562 a         1103 c         1122 g          653 t
ORIGIN      
        1 gatccccacc tcgttcctga tgggattcat ggtgctgaac ggcgtgggct acacgctcaa
       61 tttcatgatc atgttcgccc tgcttctggc cgtgggcatc ctggtcgacg gcgcgatcat
      121 cgtcgtggaa tatgccgacc gcaaaatgac cgagggccat ccgccgcgcg aagccttcgc
      181 ggaagcggcg cggcgcatgt tctggccggt ggtcagcgcc acggcgacca tgatcggcgc
      241 cttcgtcccc atgctgctgt ggcccggcat catcggcagc tacatgagct tcttcccgat
      301 cacgctcatc atcgtgctgt cgtcctccat ggtggtggcg ctgatcttcc tgccggtcct
      361 gggcggctgg ttcggccgcc cgccgccgcg cgacgaagaa caccaacgct cgatcgaggc
      421 gtccgaaacc ggcgactggc gggagatttc cggcatcacc ggctggtatg cccgtctctc
      481 ggagaagctg agccagcgtc ccggcagagt gctgctgggc gcattcggca tcgtcgtggc
      541 cgtgatcgcg ttcgcggcgg ttttcttcaa gggcgtcgag ttcttcgtca ccaccgatcc
      601 cgagatggcg aatatctacg tctcggcgcg cggcaatctc tcggcggccg agaagcgcga
      661 tctggtgatg agcgtggagc gccgcatcga ggatgtggaa ggcctgcaat acctctatgc
      721 caagaccggc ggcggcggcc agagagcgcc cgtggacgtg atcggccgca tcgtcgtcga
      781 aatgaagcca aaggcggagc gcgcccgcag cggcaaggca atcctggaag aggtgcgcaa
      841 gcgcaccgag ggcatggccg gcatccgcgt cgaagtgacc gaaccgcaat ccggccccag
      901 cggaggcaag gatatcgtcg tcgacgtgac ttctgaggac tatgtatcgc tgttgcgggt
      961 ggtcgatgcc ttgcgccgcc acatggacag ccagccggaa ctgcgcgacg tggaggacac
     1021 ccgcgcgctt ccgggcatcg aatgggactt gaagatcaat cgcgagctgg cgagccggtt
     1081 cggcgtcaac acgttgatga tcggcaccgc ggtgcagctc gtcaccaacg gcatcttcgt
     1141 ggcgcgctac cggcccgacg attccgagga cgaagtggac atccgcgtgc gctacccgag
     1201 tgcatggcgc ggcgtccatg cgctcgacaa tctgcgcatc gccaccggcg acggcagcgt
     1261 cgtgccgctc tcgaacttcg tgaccgtggt gccgtcgcaa ctgaccaacc agatcgaaca
     1321 tgccgacggc cggcgcgttt atcacgtgcg cgcgaacatg aagccgaaca cgcttctgcc
     1381 caacgccgag atggcgaagc tgaagacgtg gatcgaggcg cagaacttcc ccaccaccgt
     1441 gcataccgcg ttcaaaggct cgaccgagga ccaggacgaa tccatggcgt tcctgggcaa
     1501 agccgccctg atggcggtgt tcctgatcgc catcgtgctg ctggcgatgt tcaacagcgt
     1561 ctatcacacg ctcctcatcc tcgtcgccgt ggtactggcg atgatcggcg ccgtgctggg
     1621 catggtggtc atgggccaga ccttctcggt ggtgatgacc ggcaccggtc tcatggcgct
     1681 ggcgggcgtc gtggtgaacc acaacatcgt gctgatcgac accttccacc gccacatcaa
     1741 gtcgggcatg gatcccatcg aagcggtgat ccgttcgagc gcacagcgcc ttcgcccggt
     1801 gttcctcacc accgtcaccg ccatcggcgg cctgttgccc atgatgttcg ccgtcgagat
     1861 caatttctgg catcgcacca tcgaggtggg cagcgccaac gcctcgatgt gggtccagct
     1921 ttccaccgcg gtgatcttcg gcctcgcctt ctccaaggcg atcacgctgg ggctggtgcc
     1981 ggcgttgctg gcgctgccct accgcgtccg cgagcgtcac ggcagcttcc tcgccatgtt
     2041 cgccacgctg ccgggccgcc tcggccgggc attgctgcgg ccgtggcgct cgttgcgccg
     2101 ccgcctcgga ctcgctgcgc agccggacgc cgcgccggcg gagtgatcag tcggcggcgg
     2161 caagcggtac tatgcgggcg acgcccggcg taacctgcgg atcggcctcg atcttcttga
     2221 tgcgctcgcg ggcggcggcg gccttcttct gcttgttcca catggaggca taggcgcctt
     2281 tgcgggcgat gaggtcgtcg tggcggccgc gctccacgat ctcgccatgg tcgagcacca
     2341 ggatttcgtc ggcgtccacc accgtcgaca gccggtgggc gatcaccagc gtcgtccggt
     2401 tcttggatat ctcctgcaag gcgccctgga tttcgcgttc ggtgtgggtg tcgagcgccg
     2461 aggtcgcttc gtcgagcagc aggatcggcg ggttcttgag gatggtgcgg gcgatggcga
     2521 cgcgctgttt ctcgccgccg gaaagcttca agccgcgttc gcccaccatc gactcgtagc
     2581 ccagcggcag atcgccgatg aacttgtcga tctgggcgtg acgcgccgct tccttgattt
     2641 cggcctcgct ggcgccgatg cggccatagg cgatgttgta gcgcaccgtg tcgttgaaca
     2701 gcacggtgtc ctgcggaacg atgccgatcg ccgcgcgcaa cgaggcttgg gtcacgtcgc
     2761 ggatatcctg gccgtcgatg gtcacgctgc cgcccttgat gtcgtagaag cggaagagga
     2821 tgcgcgaaat cgtcgatttg cccgcgcccg acggaccgac cacggcgatg gtcttgccgg
     2881 ccggcaccgt gaagctgacg cccttgagca ccgtgcggtc cggatcgtag gcgaagacga
     2941 cgttgtcgaa gcgaatttcg ccgcccgaaa tcgcgagcgg cttggcgtcc ggcttgtcct
     3001 tcacttcctg cggctgatgc agcagatgga acatggcgtc catgtccacc agcgcctggg
     3061 tgatttcgcg atagacggtg cccaagaggt tgagcggcac gtaaagctgg atcaggtagg
     3121 agttggccat caccaggtcg ccgaccgaga aacggccgtg cgcgacggcc accgcggcca
     3181 gccccatgat caccgccaat cccagcgcca cgatgacggc ctggccggtg ttgaggacgg
     3241 acagcgaaat ctgcgtctga accgaggcgt cggcgtattt cttcatcgag acgtcaaagc
     3301 ggcgcgtttc gtgcgtctcg ttgttgaaat acttcaccgt ctcgtagttc agcaggctgt
     3361 ccaccgtctt ggtgttggcc tcggtgtcgc tctcgttcat caccctgcgg aactgaatgc
     3421 gccagcgcgt gatgatgaag
//
LOCUS       DASWPU010000401         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_95313,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000401 DASWPU010000000
VERSION     DASWPU010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95313"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VGC27_08650"
     CDS             complement(<1..322)
                     /locus_tag="VGC27_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282678.1"
                     /translation="MATASPAYSLPQIEAFGQLPTISSAKLSPDGMHLAAIQSQNGRP
                     VVTIYDLGAAPGTPPVILPYEEKDGHIVSIMWVKKDRLFITININFKDEDGRKVNSWY
                     RTQSV"
     gene            complement(478..1338)
                     /locus_tag="VGC27_08655"
     CDS             complement(478..1338)
                     /locus_tag="VGC27_08655"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY0282679.1"
                     /translation="MRLRFLALGIALCTLPLAGVAHAAGGAGANRDQQPSSGLQMAMT
                     LYAGGITIGRVDMDAKIVGNKYHVVSNLETSGVVNAFWQSVIQASSSGKIEDNLLQPT
                     LYDSFYTGHNTEKHQEVSLTYENGTPVRLYANPPYSTTGYEVKPEDKKATFDPLSAVV
                     FIASGVGAKEANPCSVLAPVYDGRRRYNIEMRKVKDTTVKMDNGLYKGPAVHCEIKYK
                     QLSGFKPRVLKDANFPLINAWVATFPSAVSGRSYAVPLRVWAQSQYGVVAAVATSFKI
                     DGAAPKGSGG"
     gene            1577..1870
                     /gene="rpmB"
                     /locus_tag="VGC27_08660"
     CDS             1577..1870
                     /gene="rpmB"
                     /locus_tag="VGC27_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008756960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY0282680.1"
                     /translation="MSRRCELTGKGPLVGHNVSHANNKTKRVFRPNLQVISLTSEALG
                     QAYKLKICMSALRTIDRTGGLDAFLLTKAKDDVLSPRALRLKRLVAKKAAPAA"
     gene            2001..2777
                     /locus_tag="VGC27_08665"
     CDS             2001..2777
                     /locus_tag="VGC27_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282681.1"
                     /translation="MRAFQQQVTAGGRYGVVGRVASTGVVEDGPYRLRNASDSAWAGY
                     AAYGQRETFWRKTGAVLGAILRMVMPALALLASFIGLYLYRDTPLPLFLDGTASWLTA
                     GHLLVPVGFFCVHLTNRRYGPAYALALVAAVPALIVGVLFFAAGQIMQFVPQDTVPPM
                     REAIGFGGAFVFASLVSIVVFDGARGAHWWTAPLFGFVTAAVFFAWTFCPAVYAGTGA
                     PWFDHALVYAALLAGEGVLLLIPYWMLRGIVPPISGFGGY"
     gene            complement(2904..>3345)
                     /locus_tag="VGC27_08670"
     CDS             complement(2904..>3345)
                     /locus_tag="VGC27_08670"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEY0282682.1"
                     /translation="GGQQSLVTAALNPERITAVVVNEPSGADSNGELHGRKIAYPFWP
                     SGDPQVMKTARYFDTVNFASRIEAPTLLSIGFIDSIVPPAGLWTVLNQIPAPKEVVPM
                     VESDHNNYTPDKQGAYLQRSEEVFATILHGGKFIPNERLTRPRP"
BASE COUNT          570 a         1001 c         1063 g          711 t
ORIGIN      
        1 ccacggattg cgtccggtac cacgaattga ccttgcggcc gtcttcgtcc ttgaaattga
       61 tgtttatcgt gatgaacagc cggtcctttt tcacccacat gatcgagacg atgtggccgt
      121 ctttctcctc gtatggcaga atcacgggtg gtgtgcccgg cgctgcgccg aggtcataga
      181 tcgtcaccac gggccggccg ttctgggatt gtatcgcggc cagatgcatg ccgtccggcg
      241 acagctttgc gctcgagatg gtgggaagct ggccgaaggc ttcgatctga ggaagggaat
      301 aagcgggcga tgccgtcgcc aataaagtac aaacaactac tgcgacacag cgcgccgcac
      361 gccgaacatg caccatgacc cgagcccccg cgtccccacg catgtacgag cctaacgcca
      421 accgctcttg ggcgccagca aactaacgca ttggttgagg tgccgaggca aatagcctca
      481 accgccgctg cctttcggcg ccgccccgtc gatcttgaac gaggtcgcga ccgcggcgac
      541 cacgccgtat tgcgattgcg cccagacgcg cagaggaacc gcataggagc gtccggagac
      601 ggcgctcggg aaggtcgcca cccaggcgtt gatcagcggg aagttggcat ccttcagaac
      661 cctcggtttg aagccggaga gttgtttgta tttgatttcg cagtggactg cgggaccctt
      721 gtagagcccg ttgtccatct tgacggtcgt gtccttgacc tttctcattt cgatattgta
      781 gcggcggcgg ccgtcgtaaa ccggcgccag cacgctgcaa ggattggcct ccttcgcacc
      841 gacaccggag gcgatgaaaa cgaccgcgct caagggatcg aatgtcgctt ttttatcctc
      901 cggcttcacc tcgtatccgg tcgtgctgta gggcggattg gcatagaggc gcaccggcgt
      961 accgttttcg taggtcagcg aaacttcctg gtgcttttcg gtgttatgcc cggtgtagaa
     1021 agaatcgtag agcgtgggct ggaggaggtt gtcctcgatc ttgccgctgg agctggcctg
     1081 aatcaccgac tgccagaagg cgttgaccac gccgctggtc tccagattcg agaccacgtg
     1141 atacttgttg ccgacgatct tggcgtccat gtccactctg ccgatggtga tgccgccggc
     1201 atagagcgtc atcgccattt gcaggcccga agacggctgc tggtcccggt ttgcgccggc
     1261 gccgccggcg gcatgcgcca cgccggcgag gggcagagtg caaagggcga tgccgagtgc
     1321 aagaaaacgg agtctcatcg ctggctattc tccgggggtg accgagtttt gcagggttgc
     1381 ggctttctat ggttcaatta tgacgcccga gaaggcaagg cagaacctct tgcttgccga
     1441 aaagttaatc cggacgagga cttgggggcc gtccggcggc ggtaatgccg caccgtggct
     1501 tgaccggggc ggtccccgca attaagagag cgcctctttc ttgcttcgcc taggattgcg
     1561 cccaaggaac cccgccatgt cccgccgttg cgaattgacc ggaaaaggcc cgctggtcgg
     1621 ccataatgtc agccatgcca ataacaagac caagcgtgtc ttccgcccga acctacaagt
     1681 tatatcgctc accagcgagg ctttgggcca ggcctacaag ctgaagatct gcatgagcgc
     1741 cctgcgcacc atcgaccgca cgggcgggct ggatgcattc ctgcttacca aggccaaaga
     1801 cgacgttttg tcgccccgcg ccctgcgtct gaagcgcctg gtcgcgaaga aggccgcccc
     1861 ggccgcctga cgggcacgat aaacgccgca tttactttgc tttgccggta attcggcatg
     1921 gcgcgtgcag ccatattccc gtaatgtatc aaaatgtgtc aggccgccgg ttgagcggcc
     1981 ggcgcaaggt ggggtgtgcg gtgcgggcgt ttcaacaaca agtgacggcg ggcgggcgct
     2041 acggcgttgt cgggcgggtg gcttcgaccg gagtcgtgga agacggtccc tatcgtttgc
     2101 gcaatgcaag cgattctgcg tgggccgggt atgcggccta cggccagcgc gaaaccttct
     2161 ggcggaaaac cggcgcggtg ttgggcgcca ttctgcgcat ggtgatgccg gctctggcgc
     2221 ttttggccag cttcatcggc ttatatcttt atcgcgacac gcccttgccg ctgtttctgg
     2281 atggcacggc atcctggctg acggccgggc atttgctggt gcccgtcggc ttcttctgcg
     2341 ttcacctcac caaccggcgc tacggaccgg cttacgccct cgcgctggtc gctgccgtcc
     2401 cggcgctgat cgtcggcgtg ctgttctttg ccgccggcca aatcatgcaa ttcgtgccgc
     2461 aggacaccgt accgccgatg cgcgaagcga ttggcttcgg cggggcgttc gtttttgcca
     2521 gcctcgtctc catcgtcgtg ttcgacggcg cgcgcggcgc gcattggtgg acagcgccgc
     2581 tgttcggctt cgtcaccgct gcggttttct tcgcctggac attctgtccg gccgtttacg
     2641 ccggaaccgg cgcgccatgg ttcgaccacg cgctcgtcta tgccgcgctg ctggcgggcg
     2701 agggcgtctt gctgctgatc ccctactgga tgctgcgcgg catcgtgccg ccgatctccg
     2761 ggttcggggg atactagaaa cgctttcaga cggagtcacc ccgcaccctt cgacaggctc
     2821 agggtgaggg tttttcttcc cctcatgctg agcttgtcga agcatgagta tggatcacgc
     2881 tcttaagcgc cgcgcggccc atcctacggt ctgggccggg tcaatctctc gttcgggatg
     2941 aatttgccgc cgtgcaggat ggttgcgaag acttcttccg agcgttgcag ataggcgccc
     3001 tgtttatccg gcgtgtagtt gttgtgatcg gattcgacca tgggcacgac ttccttgggg
     3061 gccggtatct ggttcagtac ggtccaaagg ccggccggcg gaacgatgga gtcgatgaac
     3121 ccgatcgaaa gaagggtcgg cgcttcgatg cgcgaagcga agttgaccgt atcgaaatag
     3181 cgcgcggtct tcatgacctg cggatcgccg gagggccaga acggataggc gatcttgcgg
     3241 ccgtggagtt cgccgttgga atccgcgccc gacggttcgt tgacgaccac ggcggtgatc
     3301 cgttcgggat tcagtgcggc cgtgaccagg ctctgctggc cgccc
//
LOCUS       DASWPU010000402         3536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_52193,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000402 DASWPU010000000
VERSION     DASWPU010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52193"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VGC27_08675"
     CDS             <1..196
                     /locus_tag="VGC27_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282683.1"
                     /translation="RDRTVDGLAAALAEAAADRARAAAFGIEARKRAETKFRVEDMCE
                     AYGAVYRELAGAAPAGAGSA"
     gene            193..1479
                     /locus_tag="VGC27_08680"
     CDS             193..1479
                     /locus_tag="VGC27_08680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0282684.1"
                     /translation="MTSQTNAPTFDAAAPLRRLRVLFINDTSRNGGPGRTILYILKFL
                     DPARIHRTVLIPREGIVSRRLADAGAAESLFFEPGLIENLYEPLSRPIEREDFKAPLV
                     LKMARAAGNLVRATAGFVRLRRRIRKERFDLIFCNGTSAGFLGGALAAVTGVPAIWHV
                     LYPSVAPLIRPLHRKLAAGKNVRLIICVSKPTEPQFDHCREKVRAIQTALDIDEFDGR
                     GTEPVLRRELGFDRQTVVFGSHGRILPRKGFIELIRAARIVVDRLSPEERARCRFVVL
                     GDTPQDMRPDHLEECRALVREMNLADQVQFIGFRPDVRSYVADFDVAVVPSVYEDPLP
                     RAVMESMAMSKPVVAFAMGGIGEMIDDNVEGRLARGRPPDIAGLAEACLAYFHDPQMR
                     RRHAAAARLRVERDFDARKHAGRLQDDMFRIVGSAP"
     gene            1737..2012
                     /locus_tag="VGC27_08685"
     CDS             1737..2012
                     /locus_tag="VGC27_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY0282685.1"
                     /translation="MANIREDVIEIVAKQAKVDGATLTLDTHLSELNLQSIDIVELVF
                     AIEEKFDIEVPYSPSDMNTAGISFDTVGDIIDAVERMTAEQHAKKAV"
     gene            2065..3270
                     /locus_tag="VGC27_08690"
     CDS             2065..3270
                     /locus_tag="VGC27_08690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY0282686.1"
                     /translation="MNRVVITGLGIVSPLGNSVTDVAASLRAGRGAIGPLTLFPTDGL
                     ITKIGAEVRDFDPAAHFSDKQLPLLDRCAQLALVAARQAVAQSGLSFRDGLGSRAATI
                     IGSGVGGMNTLDESFHRVYAERAKRVHPFTVPKLMISAAMSHITMEHGITGPSFTVAS
                     ACASANHAMGVAYHMVRAGSVDVAVTGGTESVFTFGAIKAWEALRVMALDLCRPFSRN
                     RSGMVLGEGAAIVVLENRARAEARGAEILGEIVGFGMSSDASDIVLPSAAGAASAMRA
                     CLADAGLAPEDVDYINAHGTGTVANDLTETKAIHMVFGDHAKKLAVSSTKGMHGHALG
                     AAGALELAATVIAMQEGFIPPTTNFTEADPECDLDYVPNQARAARIEVALSNSFAFGG
                     HNAVLAIRR"
     gene            complement(3318..>3536)
                     /locus_tag="VGC27_08695"
     CDS             complement(3318..>3536)
                     /locus_tag="VGC27_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282687.1"
                     /translation="FICKLRKKLAGVMDGDDYIRTVWGLGYAFCGPALSRRQLPRRQM
                     RAGTSSMLDGPASSAPDRKLSGYGLSTQ"
BASE COUNT          648 a         1138 c         1121 g          629 t
ORIGIN      
        1 gcgggaccgc acggtagacg ggctggctgc ggcgctcgcg gaggcggcgg ccgaccgtgc
       61 gcgtgcggcc gctttcggta tcgaggcccg caagcgggcg gaaacgaagt tcagggtgga
      121 agacatgtgc gaagcttacg gcgccgtata ccgcgaactt gcaggagccg cgccggcggg
      181 ggcaggctcc gcatgacttc gcagaccaac gctcccacgt ttgacgccgc ggcgccatta
      241 cgacggctgc gggtgctgtt catcaacgat acatcgcgca acggcggtcc cggccgtacg
      301 atcctttaca tcctgaaatt cctcgacccg gcacgcatcc accggacggt gctgattccg
      361 cgcgaaggca tcgtcagccg cagactcgcc gacgccggag ccgcggagtc tctcttcttc
      421 gagccgggcc tgatcgagaa cctgtacgag ccgctgtcgc gcccgatcga gcgggaagac
      481 ttcaaggcac cgctggttct gaagatggcg cgggcggcgg gaaacctggt tcgcgccacg
      541 gcaggcttcg tccggctgcg gcgccggatc cgcaaagaac gcttcgatct gatcttctgc
      601 aacggaacct cggccggctt cctgggcggc gcgctggcgg cggtcaccgg cgtgccggcg
      661 atctggcatg tgctgtatcc ctccgtggcg ccgctcatcc gcccgttgca ccgcaaattg
      721 gccgcgggca aaaacgtccg cctcatcatc tgcgtctcaa aacctaccga accgcaattc
      781 gaccattgcc gggagaaagt acgcgccatc cagaccgcgc tcgacatcga cgagttcgat
      841 ggacgcggca cggagccggt gttgcgccgc gagcttggct tcgaccggca gacggtggtt
      901 ttcggaagcc acggccggat tctgccgcgc aaaggcttca tcgagttgat ccgcgccgcg
      961 cgcatcgtcg tcgacaggct gagccccgag gagcgtgcac gctgccgttt cgtcgttctc
     1021 ggcgacacgc cgcaggacat gcggccggat catctcgaag agtgccgggc cctggtgcgg
     1081 gagatgaacc tcgcagacca ggttcagttc atcggcttcc gccccgacgt cagatcctat
     1141 gtggcggatt tcgatgtcgc ggtcgttccc agcgtttatg aagaccccct gccgcgtgcc
     1201 gtcatggaat cgatggcgat gtccaaacct gttgttgcat tcgccatggg cggcatcggc
     1261 gaaatgatcg acgacaatgt agagggccgt ttggcgcgcg gccggccgcc cgatatcgcc
     1321 ggattggccg aggcatgttt ggcgtatttt catgatccgc agatgcgccg ccggcacgcc
     1381 gccgccgccc gcctgcgggt cgagcgggat ttcgacgcca ggaaacatgc aggcaggttg
     1441 caggacgaca tgttccgcat cgttgggagt gcgccgtagc ttgccgcgcc gttcgccgcc
     1501 cggaaagtca catgccgcca ccgtgcgccg caagtccggc caagcgccgc atcgtccgtt
     1561 ttgcagaacg gatgtgtcgc agcccctcgc gagagccgag aggcgcggtt gtcgcggaat
     1621 ccacagcaaa attgaagcag cccagatcga catttgccta taatggacaa acggttacaa
     1681 tgccgtgccg acgaatggaa ttgcatgaga cttcatcaaa ggggggtcgc gacgacatgg
     1741 cgaatataag agaagacgtt atcgaaatcg tcgccaaaca ggcgaaagtc gatggcgcca
     1801 cgctgacgct tgacactcat ttatccgaac tcaacctgca atccatcgat atcgtcgagc
     1861 ttgttttcgc gattgaagaa aagttcgata tcgaagtgcc atattcaccc agcgatatga
     1921 acacggccgg catctcgttt gataccgtcg gggacattat cgatgccgtc gaacgcatga
     1981 ccgccgagca gcacgcgaaa aaagcagtgt gacgggcttc ccccttcgtt tccgataaaa
     2041 ttcagctttg aaaggcgtgc cggcatgaac cgggtggtta ttacgggtct tggtatcgtt
     2101 tcgccgctcg gcaacagcgt gacggacgtc gcggccagtc tgcgcgccgg gcgtggcgcc
     2161 atcggacctc tcaccctctt cccaaccgac gggctgatca cgaagatcgg ggcggaagtt
     2221 cgcgacttcg atccggccgc ccacttctcc gacaagcaac tgccgctgct cgaccgctgt
     2281 gcccagctcg cgctggtggc ggcgcgccag gcagtggcgc agagcggctt gagctttcgc
     2341 gacgggctgg ggtctcgcgc agccaccatc atcggctccg gcgtcggcgg catgaacacg
     2401 ctcgacgaga gttttcaccg tgtctacgcg gaaagggcaa aacgggtcca tcccttcacc
     2461 gttcccaagc tgatgatctc cgcagcgatg agccacatca cgatggagca cggcatcacc
     2521 ggaccctcct tcaccgtggc gagcgcctgc gcctccgcca accacgcaat gggggtggca
     2581 tatcacatgg tgcgtgccgg ctcggtcgat gtcgcggtca ccgggggcac cgagtcggtg
     2641 ttcaccttcg gcgcgatcaa ggcctgggag gcgctgcgcg tcatggcgct cgatctctgc
     2701 cggccgttct cgcgcaaccg ttcaggcatg gtgctcggcg agggcgcggc aatcgtggtg
     2761 ttggagaatc gcgcccgagc cgaagcgcgc ggcgccgaaa tcctcggcga aatcgtcggc
     2821 ttcgggatga gctccgacgc cagcgacatc gttttgcctt cggcggcggg agcggcgagc
     2881 gccatgcgag cctgccttgc cgatgccggc ctcgcccccg aggacgtcga ctacatcaac
     2941 gcccatggca ccggcacggt ggccaacgat ctcactgaga ccaaggcaat ccatatggtt
     3001 ttcggagacc acgcgaagaa gctcgcggtg tcctcgacca aagggatgca cggccatgcc
     3061 ctcggcgcgg cgggcgctct cgaactcgcc gcgacggtca tcgcgatgca ggaaggtttc
     3121 attccgccga caaccaattt caccgaggcc gatcccgagt gcgacctgga ctatgttccc
     3181 aaccaggcgc gcgcggcacg gatcgaagtg gcgctgtcga attccttcgc gttcggcgga
     3241 cacaatgccg ttctcgcgat ccggcgctga ccgccgcccg gcggggacgc aacgcatcgg
     3301 cctcttcctt cggctcgcta ttgcgtgctc aaaccgtagc cggagagttt acggtcaggc
     3361 gcggatgaag ccggcccatc aagcattgag gacgtcccgg ctctcatttg gcgccttgga
     3421 agctggcgac ggctcaacgc ggggccgcag aacgcgtagc cgagccccca cacggtccga
     3481 atatagtcgt cgccgtccat cacgccggcg agcttcttac gcagcttgca gatgaa
//
LOCUS       DASWPU010000403         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_52196,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000403 DASWPU010000000
VERSION     DASWPU010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52196"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VGC27_08700"
     CDS             complement(<1..339)
                     /locus_tag="VGC27_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282688.1"
                     /translation="MLLASVGAAMDRADTKNLRVLIVGGKPHAVAILRTAFGILGLNR
                     VSVIARSERAIQLMRDEDVDAVFCDEAAGAVDGLPFPLAARRAEGVLNPMLPIFLVCG
                     SPVRRQIESAR"
     gene            508..831
                     /locus_tag="VGC27_08705"
     CDS             508..831
                     /locus_tag="VGC27_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282689.1"
                     /translation="MRNTVRIAVALLVALSLAGCGIFFPSAASRAGRDTPGFRAGYSD
                     GCASANSQDTNYRNDQVRDEAAYAADRHYRSGWASGFSACRTYTTRDPSSGPIPDNNP
                     GGRSY"
     gene            844..1092
                     /locus_tag="VGC27_08710"
     CDS             844..1092
                     /locus_tag="VGC27_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282690.1"
                     /translation="MIRALIWTPILLVVGALAAIYIAYGHSDPCRALAVEKARRTLGS
                     ASGLAEPFTRLGTSQMSSTACARKLIDSWRQRISDGAS"
     gene            complement(1490..1729)
                     /locus_tag="VGC27_08715"
     CDS             complement(1490..1729)
                     /locus_tag="VGC27_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282691.1"
                     /translation="MHKMIAAAIADVRTDFAPARDIHMPVLEQNRSGPGSVDFDLNSP
                     WVAFEPPIHGMAVHTLSLWTPPGPGEGRVTVVNSR"
     gene            complement(1779..2189)
                     /locus_tag="VGC27_08720"
     CDS             complement(1779..2189)
                     /locus_tag="VGC27_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0282692.1"
                     /translation="MNRIFTIRQLTKEFSVTARTLRFYEDEGLIAPERRGQTRIYSVR
                     DRARIILILRGRRLGFSLAEIREYLDLYDRNSNSKQLLHARRKFEERIAAYERQKADI
                     DASIIELKRSIVEVEDRLATQAREAAPLLAVAAE"
     gene            complement(2465..3094)
                     /locus_tag="VGC27_08725"
     CDS             complement(2465..3094)
                     /locus_tag="VGC27_08725"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY0282693.1"
                     /translation="MASPFSLSPLPWEDNALEPVVSARTISFHYHKHHKTYVNTLNTL
                     VEGTPYAEMKLEEIVHKTAADAAAKKIFNNAGQVWNHDFYWRSLASKASAPQGDLARA
                     VERDFGSAAKLIEALADAGKTQFGSGWAWLVSNGGKLSVVSTPNAESPMAKGVNCLLT
                     VDVWEHAYYLDYQNERPRYLETVLAKSLNWNFAAANLAKESHPVRSAVE"
BASE COUNT          616 a         1013 c         1011 g          649 t
ORIGIN      
        1 gcgcgcgctt tcgatctggc ggcgcaccgg cgaaccgcac accagaaaga tcggcagcat
       61 cggattgaga acgccttcgg cgcggcgcgc ggccaaggga aacggcaagc cgtccacggc
      121 gccggccgcc tcgtcgcaga acacggcatc gacgtcttcg tcgcgcataa gctggatcgc
      181 gcgctcggat cgggcgatca cgctcacccg gttgagaccg aggatgccga acgccgtgcg
      241 cagaattgcg accgcgtgcg gcttgccgcc cacgatcagc acccggagat tcttggtatc
      301 cgccctgtcc atggccgccc cgacactcgc caaaagcatg ccgccagcgc cgttaaaccg
      361 cggttaaggc ttgcgcccgg ggagcgaagg acaaggtgcg aagtggaaac ggcgaacggg
      421 cgagccttgc cgccgttcag cttaatcttc gaccgttcca tcattgaaag cctgttgccc
      481 ggcggcgcga aatgctagcg tgggcccatg agaaacaccg tccggatcgc cgtcgccctt
      541 ctcgtcgcgc tctcgcttgc gggctgcggc atcttctttc ccagcgccgc ctcgcgcgcc
      601 ggcagggaca cgccgggctt cagggccggc tacagcgacg gctgcgcctc ggccaacagc
      661 caggacacca actaccgcaa cgaccaagtg cgcgacgaag ccgcttatgc ggccgacagg
      721 cattatcgct ccggctgggc tagcggcttt tccgcctgcc gcacctacac gacgcgcgat
      781 ccttccagcg gtccgatccc cgacaacaat cccggcgggc gcagctactg aaaggcggcg
      841 gcgatgatcc gcgctttgat ctggacaccg atcctgctgg tcgtgggagc cttggccgcc
      901 atttacatcg cgtacggcca tagcgatccc tgccgcgcgc tggcggtcga aaaggcccgc
      961 cggacattgg gatcggcgag cggactggcc gaacctttca cgcggctggg gaccagccaa
     1021 atgtcgtcca ccgcctgcgc ccgcaagctc atcgactcct ggcgccagcg gatttcggac
     1081 ggcgcaagct aaggcgcaat ccatccagtt cggatcgtgt caggctcatg cttcgacaag
     1141 ctcatgcttc gacaagctca gcatgaggga taaggcactc ccttcaccct gagcctgtcg
     1201 aagggcggtg gggacaattc aactggattg gatcagatcc aagcacccct cacgcaaccg
     1261 ccgcgaattc ttaatctctc attaaccttt gcgcgcgatc ttccgtggac ggattgtcgc
     1321 gggccggatt tcgtgtatac tccgtgcaac tattcgaggg cgcacctgcg atgctgggac
     1381 atctggtata cagccgcacc gattactgga gcgacggcaa gagccgtagt ttcgctccgt
     1441 tgaaatggtt gcggcgcgtg ctgagcgctc tgttccgccg gcactgagat catcttgaat
     1501 tgaccaccgt cacccgtccc tcaccagggc cgggcggggt ccatagcgac agcgtatgga
     1561 ccgccatccc atggatgggc ggctcaaagg ccacccatgg tgagttgaga tcgaagtcaa
     1621 ccgatcctgg cccgctgcgg ttttgttcaa gcaccggcat atgaatgtcg cgcgcgggcg
     1681 cgaaatctgt gcgcacgtcc gcgatggcgg cggcgatcat cttatgcatg aaaaccctcc
     1741 ccgctggcgc ggggaggttt tagcatcctt ggaaggtttt attcagccgc tacggccagg
     1801 agcggcgcgg cctcgcgcgc ctgggtcgcc agacggtcct cgacctcgac gatgctgcgc
     1861 ttcagctcga tgatcgaagc gtcgatatcg gccttctggc gttcataggc cgcgatgcgc
     1921 tcttcgaact tgcgccgcgc atgcagaagc tgcttggaat tggagttccg gtcgtagagg
     1981 tcgagatatt cgcggatctc ggcgagcgaa aagcccaggc gccgtccgcg caggatcaaa
     2041 atgatcctgg cgcggtcgcg aaccgaatag atgcgcgtct ggccgcgccg ctcgggcgcg
     2101 atcaagcctt cgtcttcgta aaaccgcaaa gtacgcgcgg tgacggaaaa ctctttggtc
     2161 agctgacgga tcgtgaatat gcggttcatg gtcacctaca tcgttagcgc gggccacggt
     2221 gacgccgggt aatgccggtt ctaggaccgt atccctgaca atttctcaga gtacgattct
     2281 aggttgacgc cgacgtaagg gtcaagacgt tttcacgatt ttttctaatt ctaataaatt
     2341 atatatattt caatgatttc cgtgtctttc agagcaaaag gagagccgaa ttggtgcaaa
     2401 atagcatcag ggcctgaagg ggaaaagggg tcggacgccc cctttctccc tccttcttgg
     2461 ttggttattc cacggcgctg cggaccggat ggctttcctt ggccaggttg gcggcggcga
     2521 agttccaatt cagcgatttc gccagcacgg tctccagata gcggggccgt tcgttctgat
     2581 agtcgaggta ataggcatgc tcccagacat cgacggtgag caggcaattg acgcccttgg
     2641 ccatcgggct ttcggcgttc ggcgtgctca cgaccgagag cttgccgccg ttcgagacca
     2701 gccaggccca gcctgatccg aactgcgtct tgccggcgtc ggccagcgcc tcgatcagct
     2761 tcgcggcgga gccgaaatcg cgttcgacgg cgcgggccaa gtcgccttgc ggcgccgagg
     2821 ccttggacgc caggctgcgc caatagaaat cgtggttcca cacctggccg gcattgttga
     2881 agatcttctt ggcggcggcg tcggcggccg tcttgtgcac gatctcttcc agcttcatct
     2941 cggcatacgg cgtgccttcc accagcgtgt tcagggtgtt cacgtaagtc ttgtgatgct
     3001 tgtgatagtg gaacgagatg gtgcgggcgc tgaccacggg ttcgagggcg ttgtcttccc
     3061 aaggcagggg cgagagactg aaaggcgagg ccatatgcta ctccttcggc aggtttggaa
     3121 ctgtcgccgg ctccggccca agcgggcggc cgctatctgg ccacgattcg gagaaggcgc
     3181 gagcggcgaa acgctcccgc cctttcgccg gcaagtcaag aataattata atgtcacaac
     3241 gctctaatgt cgtggtttcg aagttcgcag catgcgcact atcgggctg
//
LOCUS       DASWPU010000404         3583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_113841,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000404 DASWPU010000000
VERSION     DASWPU010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113841"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2001)
                     /locus_tag="VGC27_08730"
     CDS             complement(<1..2001)
                     /locus_tag="VGC27_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0282694.1"
                     /translation="MNRLLLGASVVALTIASGAQAAEPADSDSVQVAKLETVIVTAER
                     RQESAQDVGIALSVVSADELTKRGINSVNQLQFVVPSFEAVPAFGSGQPEFRLRGVGF
                     DDYGSNNSSTVGVYVDEVAYPVPASTQGLFFDLSRVEVLRGPQGTLYGRNTTGGAINF
                     ITNRPTDTFGAGAKLQYGSYNAFRGEAYVSGPIADDLQFRLAATTEQGGAWQKNRTTG
                     ASLGDRDTSAVRGQLAWAPTQSVDFLLEANWGYDKSESPGLYLFSPLDYAPPLPPVPA
                     DTKRSNTGWGGSVAFQGLTGIAPDEKPFHDSVNQGVSLHTSVDFGVAELTSITAYQHL
                     RRREYNDWDASAYAYAGVYFNTGAEVFSQEVRLASAEAGPFTWLAGVYYSNETLDEAF
                     YSDFWQAFGFDTRTTYKQYVSSIAAFAQAEYQFTDSLKLVGGLRAEDEVRKQKGYATA
                     GVYAPGTPPADFSPPADKSLKSQPLTGKVELEYKPTETALLYASISQGVKSGGFTAYN
                     VPSSSSVQAFKPETLWAYEAGFKTTFGDGTLQLNGSVFYYDYHNQQVQSAIWTTAYGA
                     IGAIVNAEKSHLYGGELELQWRPVENLFISQNVGYKDGSFDKFNSDLDIAASQAANSA
                     VFVDRKGAKVGFPPASYQGSVSYLLHFDGYNLEPEIDYAFRDG"
     gene            2188..3204
                     /locus_tag="VGC27_08735"
     CDS             2188..3204
                     /locus_tag="VGC27_08735"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY0282695.1"
                     /translation="MITAGRKNLITDVAGLKIGNAEDERVRSGVTVLIGDRAFGAVID
                     IRGASPGTRDTSALNLTSVGFPVDALVLAGGSLYGLDAVSGVLSHLRSQGRGYTYEKD
                     IAPLPMVPGAILFDLTNGGDKDWGDVSPYAGLGRRAAQSASEHFRLGNAGAGYGARAG
                     QYKGGLGSASAVLENGRTVGALAAVNSVGSPVIPGTDVLWAYALEQDREFGGRSPRRL
                     DAIDLDLPADMKSAPSAGANTTLAVVATDAPVSRAGLQQIAIMATDGFARALRPAHTP
                     FDGDLVFAVSTETNAPDRTDPVETMLLGHVAADCVARAIARGVYEAETLGTFKSYRET
                     FGRI"
     gene            complement(3201..>3583)
                     /locus_tag="VGC27_08740"
     CDS             complement(3201..>3583)
                     /locus_tag="VGC27_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0282696.1"
                     /translation="KIAEFLAAHEDVASVDYPGLPGNRYHALAKRLLPKGAGAIFSFT
                     VKGGQARAEKFIEAVKIFSFLANVGDSKSLIVHPATVTHAQLDERQQREVGITPELIR
                     LSIGTEDPGDLIWDLNQAFDRSRD"
BASE COUNT          667 a         1123 c         1096 g          697 t
ORIGIN      
        1 cccgtcgcgg aacgcgtaat cgatctccgg ttcgagattg tagccgtcga aatgcagaag
       61 gtaagacacc gagccttgat agctggcggg cgggaagccg actttagcgc ctttccggtc
      121 cacaaacacg gccgagttcg ccgcctggct ggcggcgata tcgagatcgc tgttgaactt
      181 gtcgaagctg ccgtccttgt acccgacgtt ctgcgagatg aagaggtttt ccaccggacg
      241 ccattgcagt tccagctcgc cgccatagag gtgcgacttc tcggcgttta caatcgcgcc
      301 gatcgcgcca taggccgtgg tccagatcgc cgactgaacc tgttgattgt ggtaatcgta
      361 gtagaacacg gagccgttga gctgcagggt tccgtcgcca aacgtcgtct tgaagcccgc
      421 ctcgtaagcc cacaacgtct cgggcttgaa cgcctgaacc gaactcgaac tcggcacatt
      481 ataggcggtg aagccgcccg acttcacgcc ctgggaaatg cttgcataca gcagcgccgt
      541 ttcggtcggc ttgtactcga gttcgacctt gccggtgagc ggctggcttt tcagcgactt
      601 gtcggcggga gggctgaagt cggccggtgg cgttccaggc gcatacaccc ccgccgtcgc
      661 gtatcccttt tgcttgcgaa cttcatcttc ggcgcgcagc ccgccgacga gtttcaggct
      721 gtccgtgaac tggtattcgg cttgagcaaa cgccgcgatc gaactgacat attgcttgta
      781 ggtggtcctg gtatcgaagc cgaacgcctg ccagaaatcg gaatagaagg cctcgtcaag
      841 cgtttcgttc gaatagtaca cgcccgcgag ccaggtgaac ggccccgcct ccgccgaagc
      901 gaggcgcact tcctgcgaaa acacctccgc tcccgtgttg aaatagacgc ccgcataggc
      961 ataagccgag gcgtcccaat cgttgtattc gcgccgccgc aggtgctgat aggcggtaat
     1021 gctcgtcagc tcagcgacac cgaaatcgac cgaggtatgg agactgacgc cctggttgac
     1081 ggagtcgtgg aacggcttct catccggcgc gatgccggtc aggccctgaa acgcaaccga
     1141 tccgccccat ccggtgttgg accgtttcgt gtccgccgga accggcggca acggcggggc
     1201 gtagtcgagc ggcgagaaga gatagagtcc cggactctcg gatttgtcgt aaccccaatt
     1261 ggcttccaga agaaaatcga cgctctgcgt cggcgcccag gccaactggc cgcgcacggc
     1321 gctggtgtcc ctgtcgccga ggctcgcgcc cgtcgtgcgg ttcttctgcc atgcgccgcc
     1381 ctgctccgtc gtggccgcga ggcggaattg gagatcgtcg gcgatggggc cggacacgta
     1441 cgcttcgccg cggaaggcgt tgtagctgcc gtattgcagc ttcgcaccgg cgccgaacgt
     1501 gtccgtcgga cggttggtga tgaaattgat ggcgccgccg gtggtgttcc gtccgtacag
     1561 cgttccttgc gggcctcgca aaacttccac ccgcgaaaga tcgaagaaca agccttgggt
     1621 cgaagccggc accggatagg cgacctcatc gacatacacg ccgaccgtcg agctgttgtt
     1681 cgagccataa tcgtcgaaac ccacgccccg gagcctgaac tccggctggc cgctgccaaa
     1741 ggccggcacc gcttcgaagc tcggcaccac gaactgcaac tgattcacgg agttgattcc
     1801 gcgcttcgtg agttcatctg ccgatacaac actcagcgca atgccgacat cttgggcaga
     1861 ttcttggcgc cgctccgccg tgactatcac ggtttccagc ttcgcaacct ggaccgaatc
     1921 cgaatcggcc ggttccgccg cttgcgcacc actcgcgatc gtcagcgcaa cgaccgacgc
     1981 gcccaacaat aacctgttca ttcgcaggat ccccttcacg aaatctacac cgcccgattt
     2041 gtatttactg gagttactct atctaatcac tagagattac gcaacaatct cggcgacccc
     2101 tattgtcgca gtgtggaata tttgatttat gcggatcgcg ctgtagaaat aatagcggtg
     2161 cgagatgctc aagcgtatga ggacgaaatg atcactgcgg ggcgcaagaa tcttatcacc
     2221 gacgttgcgg gcctgaaaat cggtaacgca gaagacgagc gtgtccgcag cggcgttacc
     2281 gttctcatcg gggatcgcgc gttcggtgcc gtcatcgaca tccgcggtgc ttcgccggga
     2341 acgcgcgaca ccagtgcgct caatttgacg agtgtcggat ttccggtcga cgcgctggtg
     2401 ctggcgggcg gctcgcttta cggcctcgat gccgtatccg gcgtgctttc gcatttgagg
     2461 tctcagggac gcggttacac atatgagaaa gacatcgcgc cgcttcccat ggttcccggt
     2521 gcgatcctgt tcgaccttac aaacggcggc gacaaggact ggggcgacgt atccccttac
     2581 gcgggattgg gccgcagggc ggctcaaagc gcctcggaac acttccggct cggcaacgcg
     2641 ggtgcgggct atggtgctcg cgccgggcaa tacaaaggcg gcctgggcag cgcgtccgca
     2701 gtcctggaga acggccgcac cgtgggcgcg ctggcagccg tcaattcggt cggctcgccg
     2761 gtaatcccgg gaacggacgt cctttgggcc tatgcgctgg agcaggatcg cgaattcggc
     2821 ggacgcagcc cgcggcgcct cgacgccatc gatctggacc tgcccgccga tatgaagtcc
     2881 gcaccctcgg ccggagcgaa cacgacgctc gccgtcgtgg ccacggacgc gcccgtctcg
     2941 cgcgcgggcc tgcagcagat cgcgatcatg gcgacggacg gttttgcgcg cgcgttgcgc
     3001 cccgcacaca cgccgttcga cggcgatctt gtcttcgcgg tttcgaccga aacgaacgcg
     3061 ccggaccgca ccgatcccgt cgaaacgatg ctcctggggc atgtggcggc cgattgcgtc
     3121 gcgcgcgcca ttgcacgcgg cgtgtacgag gccgaaacgc ttggcacctt caagagctat
     3181 cgtgagacgt tcggacggat ttagtcccgg ctgcggtcga acgcctggtt caaatcccag
     3241 atcaggtcgc cgggatcttc cgttccgatg gaaaggcgta tcagctccgg cgtgatcccg
     3301 acttcgcgct gttgccgctc gtcgagctgc gcgtgcgtca ccgtggccgg atgcacgatc
     3361 agcgatttgg aatcgccgac attggccagg aaggaaaaaa tcttcacggc ctcgatgaac
     3421 ttctcagccc gtgcctggcc gcctttgacg gtgaaggaga agatcgcccc cgcccccttg
     3481 ggcagcagac gcttggccag cgcatgatag cggttgcccg gaagccctgg ataatccacg
     3541 gacgccacgt cttcatgcgc ggccaggaac tcggcgatct tct
//
LOCUS       DASWPU010000405         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_65074,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000405 DASWPU010000000
VERSION     DASWPU010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65074"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VGC27_08745"
     CDS             complement(<1..528)
                     /locus_tag="VGC27_08745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0282697.1"
                     /translation="MRALKVLALVTDAFGGRGGIAQYNRDLLSALVQGGTAASVAVVP
                     RGAPDPAVPPAGITQVSPRLGRVAYVLTALRVAFGQHFDVVLCGHLYMAPLAWLIARL
                     RGARLVVQMHGIEAWPRPARHRRAAVEAADLILCVSRYTRARVLDWTAIAPERVQVLP
                     NTVSAVFTPGDGSALR"
     gene            complement(528..2369)
                     /gene="asnB"
                     /locus_tag="VGC27_08750"
     CDS             complement(528..2369)
                     /gene="asnB"
                     /locus_tag="VGC27_08750"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY0282698.1"
                     /translation="MCGINGIFAYHPASGAPKNAELVASRDAMRARGPDGTGEWWSDD
                     RRYGAGHRRLSIIDLSDRALQPMVSTDGRYVVVFNGEIYNFPTLRAELEAQGVRFRTT
                     SDTEAILHLYARDGTEMVRRLRGMFAFGIWDNLKRGLFLARDPYGIKPMYTANDGWSF
                     RFASQVKALLAGGQVSRDPEPAGIVGFHLFGSVPEPFTLYREIRALPAGHTQWVDAEG
                     PREPRPFSNLAAILAEGARHAAPAGELREHVREAALDSVRAHMLADVEVGAFLSAGVD
                     SGSLLGLMRDAGQRDIRTITLAFEQFRGTEEDEAPLAARVAERYGAQHIVRHVGEQEF
                     IDDLPAILAAMDQPSIDGVNTWFVSKAAKEAGLKVAISGLGGDELLAGYPSFAEMPRW
                     RRRFGPFAAVPGLGRLARMLIRALAPGIARRRPKSLGLLEFADSWPGAYLLRRGLFLP
                     YELAQIAGPDVTREGLRRLQPLRRLASTLSPDPGSDVGRVCALESANYMRNQLLRDAD
                     WAGMAHSLEIRVPLVDATLLKALAPSIGGLTQAAGKAALANAPSLSLPDEIVKRAKTG
                     FGVPTRSWMAKAAGLRLPAAEAGEPKGLVSRRWSQVVLGAPAQALTS"
BASE COUNT          448 a          850 c          810 g          359 t
ORIGIN      
        1 gcgcagggcc gaaccgtcgc ccggcgtaaa aactgcgctg accgtgttgg gcaatacctg
       61 aacacgttcc ggcgcgatcg cggtccagtc gagaacgcgg gctcgcgtgt aacgcgacac
      121 gcaaaggatg aggtcggcgg cctccaccgc cgcgcggcgg tggcgcgccg gccgcggcca
      181 ggcttcgatg ccgtgcatct gcacgacgag ccgagcgccg cgcaagcgcg cgatcagcca
      241 cgcgagcggc gccatataga gatgaccgca aagcacgacg tcgaaatgtt ggccgaatgc
      301 cacgcgcagc gccgtcaata cgtaggcgac gcggccgagc cgcggcgaaa cttgcgtgat
      361 ccccgccggc ggaacggcgg gatcgggggc gccgcgcggc acgacggcga cggacgctgc
      421 cgtccctcct tgcacaagcg cgctcaggag atcgcgattg tattgcgcga tgcctccgcg
      481 cccgccgaac gcatcggtca ccagggcgag aaccttcaat gcccgcatca cgaggtgagc
      541 gcctgcgccg gcgcgccgag cacgacctgc gaccagcgcc gcgagacaag gcccttcggc
      601 tcgccggcct cggcggccgg tagccgcaga cccgccgcct tcgccatcca cgatctcgtt
      661 ggcacaccga aacccgtctt cgcccgcttt acgatctcgt cgggaagcga cagcgacgga
      721 gcattcgcga gcgcggcttt tcccgccgct tgcgtcaaac cgccgatcga cggggcaaga
      781 gccttgagca aggttgcatc gaccaggggc acgcgaattt cgagactatg cgccatgcca
      841 gcccaatcgg cgtcgcgcag cagctggttg cgcatgtagt tcgcggattc gagcgcgcag
      901 acgcggccca catcgctgcc gggatcgggc gaaagcgtcg aagcaagacg gcgcagcggc
      961 tgcaagcgcc gcaatccctc gcgcgtaacg tcgggccccg cgatctgcgc gagttcgtac
     1021 ggcaggaaca agccgcggcg cagcagataa gcgcccggcc agctatcggc gaattcgagc
     1081 agaccgagcg atttcggccg cctgcgcgcg ataccgggag caagtgcccg gatcaacatc
     1141 cgcgccaacc ggccgagtcc cggcacggcg gcgaacggac cgaaacggcg gcgccaacgc
     1201 ggcatctcgg caaagctcgg atacccggcg agaagctcgt cgcctccgag tcccgaaatc
     1261 gcgaccttga gtcccgcctc cttcgccgct ttcgagacga accaggtatt caccccgtcg
     1321 atcgaaggct gatccatggc ggcgaggatg gcgggaagat cgtcgatgaa ttcctgctct
     1381 cccacatgcc ggacgatgtg ctgcgccccg tagcgctcgg cgacgcgggc ggcaaggggc
     1441 gcttcgtcct cttcggtacc gcggaattgc tcgaaggcaa gcgtgatcgt gcggatgtcg
     1501 cgctggcccg catcgcgcat caggccgagc agcgaaccgg aatcgacgcc cgccgacaga
     1561 aacgcgccga cttcgacatc ggccagcata tgcgcgcgca cgctgtcgag cgccgcttcg
     1621 cgtacgtgct cccgcaattc gcccgccgga gccgcgtgcc gcgcgccttc ggccagaatg
     1681 gcggcgagat tggagaacgg cctgggttcg cgcgggcctt ccgcatccac ccactgggtg
     1741 tggccggcgg gcagcgcgcg aatctcgcga tagagcgtga acggttccgg cacgctgccg
     1801 aacagatgga agccgacgat gccggctggt tccggatcgc gcgaaacctg tccgcccgcg
     1861 agcagcgcct tgacctgcga ggcgaaacgg aagctccagc cgtcgttcgc cgtatacatc
     1921 ggcttgatgc cgtaaggatc gcgcgccagg aacaggccgc gcttgagatt gtcccagatg
     1981 ccgaacgcga acatgccgcg aagacggcgg accatctccg taccgtcccg cgcatacagg
     2041 tgcaggatcg cttcggtgtc ggaagtggtg cggaagcgca cgccttgcgc ctcgagctcg
     2101 gcgcgcagag tcgggaagtt gtagatctcg ccgttgaaaa cgacgacata gcgcccgtcc
     2161 gtgctgacca tcggctgcaa agcgcggtcc gacagatcga tgatcgagag ccgccgatgc
     2221 ccggcgccgt agcggcggtc gtcgctccac cactcgcccg ttccatccgg accgcgcgcg
     2281 cgcatggcgt cgcgcgacgc cacaagttcg gcgtttttcg gcgcgccgga cgctggatgg
     2341 taggcgaaaa ttccgttaat gccgcacatg gcccgtcatc gatttccatt acgcgtgcgc
     2401 cacatccaag ccgtctcgat catcttgtcg atggtcgaaa accgcggctg ccattccaac
     2461 tcgtccc
//
LOCUS       DASWPU010000406         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_27669,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000406 DASWPU010000000
VERSION     DASWPU010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27669"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VGC27_08755"
     CDS             <1..361
                     /locus_tag="VGC27_08755"
                     /inference="COORDINATES: protein motif:HMM:NF027449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0282699.1"
                     /translation="AMGCGRLFEGTPAMMHASLAKLAALPDDTEVYCGHEYTLSNGRF
                     ALTLEPGNRELQARMKEVEALRAKGEPTIPSTIGLEKRTNPFLRTGSAEIRTALKLEK
                     ADDVAVFAEIRRRKDSF"
     gene            complement(757..1431)
                     /locus_tag="VGC27_08760"
     CDS             complement(757..1431)
                     /locus_tag="VGC27_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY0282700.1"
                     /translation="MNKTVEDAMNRTVERVTRLYSKHERVADGIIHVLGVVFAINASL
                     WLLWNVTGLSVLVSVSVYCIGLLAMTGFSAAYNLMPSYRPSKRILRRLDHAAIFIMIA
                     ATYTPLAVNRLGGASGDIILAIIWAAATFGVAMKVLFPRRFEMASIVLYLLMGWLVVT
                     VIQPLAASLAAVDFWLIVAGGIVYSVGVIFYVFERIPYHRAIWHAFVLAAAALQFSGI
                     FGEFAR"
     gene            1696..2142
                     /locus_tag="VGC27_08765"
     CDS             1696..2142
                     /locus_tag="VGC27_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282701.1"
                     /translation="MEPHKDEIEAAFAETGGKGVVIARGVPGARFMMKFTPARLYVLA
                     DGEPQGHCLVEVMAEAFATGRLQSRSTLIDLTRFTGTVDWDAIRTMRKMTPRGRKSAS
                     RVAYVVRDGLFVNLIKIVKVIFPKIRHEAFISRAEAIAWLDADKAK"
     gene            2209..>2753
                     /locus_tag="VGC27_08770"
     CDS             2209..>2753
                     /locus_tag="VGC27_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY0282702.1"
                     /translation="MTAASIKAVVFDLGGVLIDWEPRLLYRKIFNGDDAKMETFLSQI
                     CTPEWNEKQDAGRPLAEATAERIAAYPEWEPEIRAFYGRWAEMVGGPIPGTAEIVREL
                     KARGVLLFALSNWSAETFPLVRDRFAELGLFEKIVLSGEHGCAKPDERYYRIGLEEFG
                     RPAESLLFIDDNRRNIVAAERL"
BASE COUNT          580 a          831 c          879 g          463 t
ORIGIN      
        1 cgccatgggc tgcgggcggt tgttcgaagg cacgccggcg atgatgcatg caagcctcgc
       61 caagctcgcg gcgctgcccg acgataccga ggtctattgc ggccacgaat acacgctgag
      121 caacggccgc ttcgcgctga cgctggagcc gggcaaccgc gagcttcagg cgcggatgaa
      181 agaggtcgag gctttgcggg cgaaaggcga gccgacaatc ccctctacca tcgggctgga
      241 gaagcggacg aatccatttc tcaggaccgg ctcggccgaa atccgcaccg cgctgaagct
      301 ggaaaaagcg gacgacgtgg cggtgttcgc ggaaatccgg cgccgcaagg attcgttctg
      361 agcaaggccc tgtttacata gtcgtcacgg tcgcgacgct gccgattttg ccccgatccg
      421 gcaggtggag ctggcgacag ccagcgacgg cgcgcaggcc gatgcgggga catcgggcaa
      481 gcgcggcgac gaaccgggcg ggcaaaatcg gcgcgtcccg aagggtttcg gcgaaaatgg
      541 tcctctgggg cgtcgaaagc ctcgagcgta ctccccggta cgctcttcgg ctttctcctg
      601 ccatagaacg attttcgcta cgaaacgcgg ctcgcgagga ctatgtaaac agggcctaga
      661 gcttgatccg atttaattga atcggatcaa gctctagatt ccttgatttt cgcgcaatct
      721 gacggcaaac cgcttcacac tttgccggat tgcgctctag cgcgcgaact cgccgaagat
      781 gccggagaat tgcaacgcgg cggcggccag cacgaaagcg tgccagatcg cccggtgata
      841 gggaatgcgc tcgaagacat agaagatgac gccgacggaa tagacgatgc cgcccgccac
      901 gatcagccag aaatccaccg ccgccagcga cgccgccagc ggctggatca ccgtcaccac
      961 cagccagccc atcaagaggt aaagcacgat gctggccatc tcgaaacggc gggggaacaa
     1021 caccttcatc gccacaccga aggtcgccgc cgcccagatg atcgccagga tgatgtcgcc
     1081 cgacgcgccg cccagccggt tgaccgccag cggcgtatag gtcgccgcga tcatgatgaa
     1141 gatcgccgcg tgatcgaggc ggcgcagaat tctcttcgac ggacggtagg acggcatcag
     1201 attgtaggcc gccgaaaaac ccgtcatcgc caacaggccg atgcaataga ccgagacgct
     1261 gaccagcacc gacaggccgg tgacgttcca caacagccac aggctggcgt tgatggcgaa
     1321 gacgacgccg agcacatgga tgatgccgtc ggccacgcgc tcgtgcttcg aataaagccg
     1381 cgtgacgcgc tcgacggtgc ggttcatcgc gtcttcgacg gtcttgttca tggcctcatt
     1441 ataaacgcga aatgcgacgt ggccatgatt accgcacagc aagattgcgt tttcttaagg
     1501 atcgcgcccc agcttggatt ggctaaaccg ccgcccggcc gcggcgaggc tgcagcaccg
     1561 atacaattaa aggtggcatt ggagcggtca tgatcgccgc cgacttggtt gccgcccatg
     1621 gaaacggcca ttacggtttg gatacgattc ttggacgcat cacggtctgc ggggacagat
     1681 ttcgggggaa ccgcgttgga accgcataag gacgagatag aagccgcctt cgccgagacc
     1741 ggcggaaagg gagttgttat cgcgcgcggc gtgcctgggg cccgcttcat gatgaagttc
     1801 acgcccgcgc ggctttacgt gctggcggac ggcgagccgc agggccattg cctggtcgag
     1861 gtgatggcgg aggcgttcgc gacgggaagg ttgcagagcc gttcgaccct gatcgacctc
     1921 acccgcttta ccggcacggt ggattgggat gccatcagaa ccatgcgcaa gatgacgccc
     1981 cggggaagga aaagcgcgtc gagggtcgcc tatgtcgtgc gcgacggact gttcgtaaac
     2041 ctgatcaaga tcgtcaaggt gatatttccc aaaatccgcc atgaagcgtt tatctcccgc
     2101 gccgaagcga tcgcctggct cgacgccgac aaagcgaagt gagcggccca gggatcgcaa
     2161 ggcgttgtgc ccggcccgcg ccgaggcaga cataaaggaa gaccggacgt gacagccgca
     2221 agcatcaaag ccgtggtttt cgatttgggc ggggtgttga tcgattggga gccgcggctg
     2281 ctctatcgca agatcttcaa cggcgacgac gcgaagatgg agacgttcct ttcgcaaatc
     2341 tgcacccccg aatggaacga aaaacaggat gcaggccgcc cgctcgccga ggccaccgcc
     2401 gaacgcatcg cggcctatcc cgaatgggaa cccgaaatcc gcgccttcta cggacgctgg
     2461 gccgaaatgg tcggcgggcc gattcccggc accgccgaga tcgtccggga gcttaaggcg
     2521 cgcggcgtgc ttctgttcgc gctgtcgaac tggagcgccg aaacctttcc gctggtccgc
     2581 gaccggtttg ccgaactcgg cctgttcgag aagatcgtcc tttcgggcga gcatggctgc
     2641 gccaagcccg acgaacgcta ttatcgcatc ggtttggagg aattcggccg gccggccgaa
     2701 agcctgctct tcatcgacga caaccggcgg aacattgtcg cggcggagcg act
//
LOCUS       DASWPU010000407         4658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_76515,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000407 DASWPU010000000
VERSION     DASWPU010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76515"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1317)
                     /gene="rocD"
                     /locus_tag="VGC27_08775"
     CDS             complement(94..1317)
                     /gene="rocD"
                     /locus_tag="VGC27_08775"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEY0282703.1"
                     /translation="MIASSNPHPASTGAFIALEERYGASNYKPLDVVLARGQGVYVWD
                     VEGKRYLDCLSSYSAVNQGHCHPRILAAMQEQAAKLTITSRAFRNDQLPLFYEELCAL
                     THSHKVLPMNSGAEAVETALKVARKWGYEVKGVPDGKAEIVVCHDNFHGRTLAIVGFS
                     TDPASRDKFGPFAPGFKAVPYGDAGALERAIGSNTVAFLVEPIQGEAGVIVPPPGYLK
                     KTRELCTKHKVVLILDEIQTGLGRTGKMLAEEHEGIEADLTLIGKALSGGFYPVSAVL
                     SNKEVMDVLKPGEHGSTFGGNPLACAVARAALRVLVEEGMIENAAKMGEYLLVALRTI
                     SSSRVKEVRGRGLMVAVELHPDAGGARKYCEVLKDRGILCKETHGNTLRIMPPLIVTQ
                     AQLDEALVQFRAVLA"
     gene            complement(1343..2227)
                     /locus_tag="VGC27_08780"
     CDS             complement(1343..2227)
                     /locus_tag="VGC27_08780"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEY0282704.1"
                     /translation="MSRKPTLIMTDAGHFEVSYVINPWMKPTAWSENRAAYFHAAQEA
                     SAALAKALAAAGAEVIRIEGAPGLPDMVFPANAAIILDRRALVARFRYPERRGEEPLF
                     LKSFESFKARGLLDTVEQFPEGCYQEGAGDCIWDATRQRFWAGFGQRSVREAATVIED
                     FFGAPVTPLELISPRFYHLDVCFLTLSGGEVVYYPKAFSREALGVIRDVVPKQDLIEA
                     SDEDAAGFCVNAVNIGRQIVMAQPKDGLRAKLNERGYSVTGIDLSPFMMSGGGAYCMT
                     LRLDRAREAAQEAKRAVS"
     gene            2380..2802
                     /locus_tag="VGC27_08785"
     CDS             2380..2802
                     /locus_tag="VGC27_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0282705.1"
                     /translation="MDDRDRQLVTLLRQNARMPVAALAQALGVSRGTVQNRINRLIEH
                     GTIQGFTIRVRSEAEPARIRAIMMIAVEGERSERVIKLLRGHPEISEIHTTNGRWDLV
                     LELNTDSLEAFDRALHRIRQINGIAGSETSLLLSTHPM"
     gene            3029..4261
                     /locus_tag="VGC27_08790"
     CDS             3029..4261
                     /locus_tag="VGC27_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0282706.1"
                     /translation="MRNTVKLSLHFPDAPARPGDKPDFSYLKIPEAGSVRRPDSATPA
                     AEMRDLAYDLIRVLDENHRAAGPWDARLAPETLRRALKFMVIDRIFEDRMFRTQRQGK
                     TSFYMRGLGEEAIAVAHAFALSADDMCFTSYRQQGLLIARGCPIIAMMNQVYSNAGDR
                     QHGVQMPLHYSYPEYGYFAMSGNLATQVSQAVGWAMASAYRGDDKVAVAFVGDGATAE
                     GDFHAGMLFASVYRVPVILSISNNQWAISTFQFVAGGEQTSFASRGVGVGLPALRVDG
                     NDFLASYAAVQWAAERARANLGATVIEFVTYRGHAHSTSDDPSRYRPAGDYAAWPLGD
                     PIDRLKRHLIALGEWSEAQHAAYEAEAAEEIRAMQVQAESVGTLDGGKRADPALLFEN
                     IFEEPDWRHKLQRKELGV"
     gene            4280..>4658
                     /locus_tag="VGC27_08795"
     CDS             4280..>4658
                     /locus_tag="VGC27_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY0282707.1"
                     /translation="MIQALNSALDVMLAKDPNVLIFGQDVGYFGGVFRVTEGLQKKYG
                     IKRCFDTPISELGIVGVGIGMAVNGLRPVVEIQFADYIYPAYDQLVSEAARIRYRSAG
                     GFIAPITVRSPYGGGIFGGETHSQ"
BASE COUNT          911 a         1474 c         1401 g          872 t
ORIGIN      
        1 cctccaccga ggggtgcagt ttccatatag gcaccgttcg gtggaattgt cagtgcggga
       61 aatcaatatt ccaattcgtt aacgtctgcg gcgttacgcc agcacagcgc ggaactgcac
      121 cagcgcctcg tcgagctgtg cttgggtgac gatcagcggc ggcatgatgc gcagcgtgtt
      181 gccgtgggtt tccttgcaga ggatgccgcg atccttcagc acctcgcagt attttcgcgc
      241 gccgccggcg tcgggatgaa gctcgaccgc caccatgagc ccacggccac ggacttcctt
      301 cacccgcgag ctggagatgg tgcgcagtgc gacgagcaag tattcgccca ttttcgccgc
      361 gttctcgatc atgccctctt ccaccagtac gcgcagcgcc gcacgcgcca cggcgcaggc
      421 gagaggattg ccgccgaagg tcgagccgtg ctcgcccggc ttcagcacgt ccatcacttc
      481 cttgttcgac agcaccgcgg acaccggata gaagccgccc gataaagcct tgccgatcag
      541 cgtcaggtcc gcctcgatgc cttcatgctc ctcggccagc atcttgccgg tgcgtccaag
      601 accggtctgg atctcgtcca ggatcagcac caccttgtgt tttgtgcaca gttcacgggt
      661 tttcttgaga taccccggcg gcggaacgat gacgccggcc tcaccctgaa tcggctcgac
      721 cagaaaggcg acggtgttcg acccaatggc gcgctccagc gccccggcgt cgccatacgg
      781 caccgctttg aaacccggtg cgaaggggcc gaatttatcg cgcgacgccg gatcggtcga
      841 aaacccaaca atggccagcg tgcggccatg aaaattgtcg tggcagacga cgatctcggc
      901 cttgccgtcc ggcacgccct tgacctcata gccccatttg cgtgccacct tcagcgcggt
      961 ttccaccgcc tcggcgcccg agttcatcgg cagcaccttg tgcgaatggg tgagcgcaca
     1021 gagttcctcg tagaacagag gaagctggtc gttgcggaag gcgcgcgagg tgatggtcag
     1081 cttcgccgcc tgctcctgca tggccgcgag aatcctggga tggcaatggc cttggttgac
     1141 cgccgaatag gaggacaggc agtcgaggta gcgcttgccc tccacgtccc agacatagac
     1201 gccctggccg cgcgccaaga cgacatcgag cggcttgtag ttggaggcgc cgtaacgctc
     1261 ttcgagcgcg atgaaggctc cggtcgacgc cgggtgcgga ttcgaagacg caatcatgcg
     1321 atccatcctt tcctccccaa acctatgaca cggcgcgttt ggcctcctgc gccgcctcgc
     1381 gcgcgcggtc gagccgcaag gtcatgcaat aggcaccgcc gcccgacatc atgaagggcg
     1441 acaggtcgat gccagtgacc gaatagccgc gctcgttgag cttggcgcgc aaaccgtctt
     1501 tcggctgggc catcacaatc tggcggccga tattgacggc gttgacacag aagccagcgg
     1561 cgtcctcatc gctggcttcg atcaaatctt gcttcggcac cacgtcgcgg atcacgccca
     1621 aggcttcgcg cgaaaaggcc ttcggataat agacgacctc ccctcccgac agcgtcagga
     1681 aacagacatc gaggtggtag aaccgcggcg aaatcagttc gagcggtgtg accggcgcgc
     1741 cgaagaaatc ctcgatgacg gtagcggcct cgcggaccga acgctgaccg aagccggccc
     1801 agaaacgctg tcgcgtcgca tcccagatgc agtcgccggc gccttcctgg taacagccct
     1861 ccgggaattg ctcaacggtg tcgagcaggc cgcgggcctt gaagctctcg aaagatttga
     1921 ggaataaagg ctcttcgccg cggcgctcgg gatagcggaa acgcgccacc agcgcgcggc
     1981 ggtcgagtat gatcgcggca ttggccggga acaccatatc gggcaagccg ggagcgcctt
     2041 cgatgcggat cacctccgcg cccgccgccg ccagcgcctt ggcgagcgcc gccgaagctt
     2101 cctgcgccgc gtggaaataa gctgcgcgat tctcggacca ggccgtcggc ttcatccacg
     2161 gattgatgac gtaggagact tcgaaatgac cggcgtcggt cattatcagc gtcggtttgc
     2221 gggacatggg cactccttga accaagcggc aagagaaccg ccttgtacgc attgtcgatc
     2281 ctggcgctgc taacgaaaag ccaacagaat gctggaaaac gtcagaaata atggcataat
     2341 gtcagatatt attgtcaaaa tgacagaatg ggctaaacca tggacgacag agaccggcag
     2401 ctcgtcacgc ttttgcgcca gaacgcgcgc atgcctgttg cggcgctggc gcaggcgctg
     2461 ggggtttcgc gcggcactgt acagaaccgt atcaaccgtc tgatcgagca cggcacgatc
     2521 cagggcttca ccattcgcgt ccgctccgaa gcggaacctg cccgcatcag ggccatcatg
     2581 atgatcgcgg tggaaggcga acgctcggag cgggtcatca agctgctgcg cggccatccc
     2641 gaaatctccg aaatccatac caccaacggc cgctgggatc tggtgctgga gcttaatacc
     2701 gacagtttgg aagccttcga ccgggcgctg caccgtatcc gccagatcaa cggcatcgcc
     2761 ggctcggaaa ccagcctgct gctctcgacc catccgatgt agggcgcggc cgtcggaccg
     2821 gcagtatggt ggtgttcttc gcgcgcttca gacggccgaa ctcgtctatt tttggaatcg
     2881 tcctaatttc gtgacgaaaa ctacaataaa ttcctattat tagcccgcaa agatagccat
     2941 tttcgcaaga ttatctcgca gatgctgagt tatacttatt cgcaagcccc cagccgggcg
     3001 gctctatgtc aggcgtggag gtgccccttt gcgcaatacc gtaaaactca gcctacattt
     3061 tcccgacgct cccgcgcggc cgggcgacaa gccggatttc agttatctca agataccgga
     3121 agcgggcagc gtccgccgtc ccgacagcgc caccccagcc gccgaaatgc gagacctggc
     3181 ttacgattta atccgcgtac tggacgaaaa ccatcgggcc gccggtccct gggacgcgcg
     3241 tcttgcgccg gagacgctgc gccgcgcctt gaagttcatg gtcatcgacc ggatcttcga
     3301 ggatcgcatg ttccgcaccc agcgtcaggg caagacctcg ttctacatgc gcggcttggg
     3361 cgaagaggca atcgccgttg cccatgcctt cgcgctcagt gccgacgaca tgtgtttcac
     3421 ctcgtaccgt caacaggggc ttttgatcgc gcgcggctgc ccgatcattg ccatgatgaa
     3481 ccaagtgtac tccaacgccg gcgaccggca gcatggtgtg cagatgccgc tgcattattc
     3541 ctatccggag tacggatatt tcgcgatgtc aggcaatctg gcgacgcagg tatcgcaagc
     3601 tgtcggctgg gcgatggctt cagcctatcg cggcgacgac aaggtcgcgg tcgcttttgt
     3661 cggcgacggc gccacggcag agggtgattt ccacgccggc atgttgttcg catccgtcta
     3721 tcgcgtgccc gtcattctca gcatttcgaa caatcagtgg gcgatatcga cgttccaatt
     3781 tgtcgccggc ggtgaacaga ccagcttcgc ctcgcgcggg gtgggtgtgg gcttgcccgc
     3841 cttgcgcgtg gacggcaacg atttcctcgc cagctatgcc gccgtgcaat gggccgctga
     3901 gcgcgcccgc gccaatctcg gcgccacggt catcgagttc gtcacctatc gcggccatgc
     3961 ccattcgacc agcgacgatc cctcacgcta tcgcccggcc ggcgattatg ccgcctggcc
     4021 gttgggcgac cccatcgacc gcctgaagcg gcacctgatc gcactgggcg aatggtcgga
     4081 agcgcaacat gccgcctacg aagcggaagc ggccgaagaa atacgcgcca tgcaagtcca
     4141 ggcggagagt gtcggcacgc tggacggagg caaacgcgcc gatccggccc tgctgttcga
     4201 gaacatcttc gaagaacccg attggcgcca caagctgcag cgcaaagagc tgggggtctg
     4261 agccgtgccc gcgatgaaca tgatccaggc gcttaactcg gcactcgacg tgatgctggc
     4321 gaaggacccg aacgtcctga tcttcggcca agacgtgggt tatttcggcg gcgtcttccg
     4381 cgtcaccgag ggtttacaga agaagtacgg gatcaaacgt tgtttcgata cgccgatcag
     4441 cgaactcggc atcgttgggg tcggcatcgg catggcggtc aacggtctgc gcccggtggt
     4501 cgaaatccag tttgccgact acatctatcc ggcctatgac cagctggtgt ccgaagccgc
     4561 gcggataaga taccgctcgg ccggcggctt catcgcaccg atcaccgtgc gcagccccta
     4621 tggcggcgga atcttcggcg gcgagaccca tagccaga
//
LOCUS       DASWPU010000408         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_9183, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000408 DASWPU010000000
VERSION     DASWPU010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9183"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VGC27_08800"
     CDS             <1..558
                     /locus_tag="VGC27_08800"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEY0282708.1"
                     /translation="NNEYAFHALAIYEHRKAFRPTLWTNEGAVNAAPRPIERTEQRWF
                     VGAHANVGGGCFDDSLAQLPFKWLERKAAALGLTFKDGFAAEQHADAAPISDSYAAFM
                     WGFYRLFTLGRAYYRPIGVPPKDEGPGVTNINETIDASVFDRWRTDNTYRPPKMQSWA
                     TSRTVDAGNISGSVRADNPAVAVAD"
     gene            783..1007
                     /locus_tag="VGC27_08805"
     CDS             783..1007
                     /locus_tag="VGC27_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282709.1"
                     /translation="MAVSTCIKCSGHGFELALFTPIGESKKLTIVQCSSCGTPIGVVD
                     PATSPQIEVLKNHVAAIDERLNRIAQALQE"
     gene            complement(1076..1462)
                     /locus_tag="VGC27_08810"
     CDS             complement(1076..1462)
                     /locus_tag="VGC27_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY0282710.1"
                     /translation="MMMDIRPLHNEQDYDWAIREVTRYFEAEPAPGTADGDRFEVLST
                     LIKAYEDKHFATPHGDPVDVLHFAIESMGKSQAELAALIGRNRASEILNRVRPLTLEM
                     IRTIGKEWNIPIDALVAPYELARAYA"
BASE COUNT          341 a          456 c          461 g          298 t
ORIGIN      
        1 aataacgaat acgcgtttca tgcgctcgca atctacgagc accgcaaggc attcaggccg
       61 acgctctgga ccaacgaagg cgcggtaaac gcggcgcccc ggccgatcga gcggaccgag
      121 cagcgatggt tcgtcggcgc ccatgccaat gtcggcggcg gatgtttcga cgactcgctg
      181 gcgcagcttc cgttcaaatg gctggaacgc aaggcggcgg cgctcggcct caccttcaag
      241 gacgggtttg cagccgaaca acacgccgac gccgcgccga tttcggattc ctatgcggcg
      301 ttcatgtggg gcttctatcg gctgttcacc ctgggccgcg cctattaccg gccgatcggt
      361 gtcccgccaa aggacgaggg cccaggcgtg accaacatca acgaaaccat cgatgcttcc
      421 gtgttcgacc gttggcgcac cgacaacacc taccggccgc ccaaaatgca atcctgggca
      481 acatcgagaa cggtcgatgc cggcaatatc tctggctccg tacgagctga taatcccgcg
      541 gtggcggttg cggattaggc ggtcgtcatg cccgggcttg tcccgggcat ccacgtctta
      601 aacagtctcg cggtgaaaga cgtggatggc cgggacgatg cccggccatg acggaaacgt
      661 cattgcgagc gccagcgaag caatccaggg ccacacaaac aaagctggat tgcttcgtcg
      721 ctgacgctcc tcgcaatgac aaatgagcta agctaacctc gccaaaaacc gggaggtgca
      781 aaatggccgt ctcaacctgc atcaaatgca gcgggcatgg tttcgaactc gcactgttca
      841 cgccgatcgg cgagagcaag aagctcacga tcgttcagtg ctcgagttgc ggcacgccga
      901 ttggtgtcgt ggacccggca acgagccctc agatcgaagt cctgaagaac cacgttgcgg
      961 cgatcgacga gcggctcaac cggatcgcgc aggcgctgca ggagtgagag ggcgtcaaga
     1021 ttacgcggat ggtagcaacg ctcggccctc atgcgccaaa acagggccga tgaagttagg
     1081 cgtatgcacg cgccagttcg tatggagcta ctagcgcgtc aatggggatg ttccattcct
     1141 tgccgatggt acggatcatt tccagcgtta ggggccgaac gcggttcaag atttcagaag
     1201 cccgattgcg cccaattagg gctgctagtt cggcctgcga tttgcccatc gactcaatcg
     1261 caaagtggag aacgtccacc ggatcaccgt gcggcgttgc gaagtgctta tcctcgtacg
     1321 ccttgataag cgtagaaaga acttcgaaac ggtcgccatc ggcggttccg ggcgctggct
     1381 cggcctcgaa ataacgggtt acttcgcgga tcgcccagtc gtaatcctgt tcgttgtgga
     1441 gaggccttat gtccatcatc acactgtctc cgcgtttatg ccgtcatact ctgcgtgcgt
     1501 accaacaaac ttgatgagag cggttttgaa cttgtaagaa aagcggacaa caaggc
//
LOCUS       DASWPU010000409         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_22169,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000409 DASWPU010000000
VERSION     DASWPU010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22169"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VGC27_08815"
     CDS             <1..572
                     /locus_tag="VGC27_08815"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY0282711.1"
                     /translation="IDAGNTNIVFAVYDGTDIRAEWRAVTKTTRTADEYAVLLSPLLA
                     LKSLSFSDFDAAIIATVVPAALFDLRQLCRRYLNCDPLVVGDPAVDLGISVRIDRPEA
                     VGADRLVNAVAAHERYKGPLIVLDFGTATTFDIIGENGDYEGGVISPGINLSAEALHQ
                     AAAMLPRVAVARTQSVIGKDTVPAMQSGL"
     gene            591..782
                     /locus_tag="VGC27_08820"
     CDS             591..782
                     /locus_tag="VGC27_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282712.1"
                     /translation="MEGLVARIKEEYGKPMTVIATGGLARVFYRQTQAIDHHDPDLTI
                     RGLVQIYARNVKHPSTSSG"
     gene            924..>2257
                     /locus_tag="VGC27_08825"
     CDS             924..>2257
                     /locus_tag="VGC27_08825"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY0282713.1"
                     /translation="MNFNAYGFVPEGARKWIVVDCGVLFGREATTPGVDVIMPDITFL
                     AERRDEVLGIVATHAHEDHIGAISYLWPTLRCPVYATPFTAKLIEGKLEEAGLKGRVR
                     LHAVPLQSRLKLGPFEIDFVSLTHSIPEPNALAIRTKLGVVVHTGDWKLDPEPLIGEA
                     ADAGVLRKLGDEGVLALVCDSTNALLPGESGSEATVRKALTDLIGTLKGRVAVTAFAS
                     NVARLDTIAKAARLHGRKLALVGRAMHKIVEAARATGYLKDFPSTVDEEEAAELPPRR
                     VLYLCTGSQGETRAALTRIASGEHQNVKLGPGDTVIFSSRIIPGNDLQIFALHNKLAA
                     LGVEVLSAEDHFVHVSGHPAQGELAQMYRWIRPRIAVPVHGELRHMSAHARLARSLQV
                     PQAINVENGHLLRLAPGKAQVIDEVPAGRIHLDGQVLVAEGARLSCARRSLG"
BASE COUNT          393 a          732 c          735 g          397 t
ORIGIN      
        1 ccatcgacgc cggcaacacc aacatcgtct tcgcggtcta tgacggcacg gatattcgcg
       61 ccgaatggcg cgcggtgacc aagacgacgc gcaccgccga cgaatacgcc gtgctgcttt
      121 cgccgctgct tgcgctgaag agccttagtt tctccgattt cgacgcggct atcatcgcca
      181 ccgtcgtgcc ggcggccttg ttcgacctgc gccagctttg ccggcgctac ctgaattgcg
      241 atccgctggt ggtgggcgat ccggcggtgg atctcggcat ctcggttcgc atcgaccggc
      301 cggaagcggt gggcgccgac cgtctggtca acgcggtggc ggcgcacgag cgctacaagg
      361 gtccgttgat cgtgctggat ttcggcaccg cgacgacctt cgacatcatc ggcgaaaacg
      421 gcgattacga aggcggcgtc atctcgccgg gcatcaatct ttccgccgaa gcgctgcacc
      481 aagcggccgc gatgctgccg cgcgtggcgg tggcgcgcac ccaatccgtc atcggcaagg
      541 acacggtgcc ggcgatgcag tcgggccttt agtggggcta tatcggtctg gtggaaggtc
      601 tggtcgcgcg catcaaggaa gaatacggca agcccatgac ggttatcgcc accggcggcc
      661 tggcgagagt gttctaccgg cagacgcagg ccatcgacca tcacgatccc gatctcacca
      721 tccgcggcct ggtgcagatc tacgcgcgca acgtgaagca cccttcgaca agctcagggt
      781 gaaggtttat tcaatccctc atgctgagcc ctcatccaga gcttgtcgag ggacgaagca
      841 tgaagggagc agtattttga ttggatcata tgcacaagga tgaattagtc cttctcccgc
      901 tcggcggggc gggcgagatc ggcatgaatt tcaacgccta cggcttcgtc ccggaaggcg
      961 cgcgcaaatg gatcgtggtc gattgcgggg tgctgttcgg gcgcgaggcc acgacgcccg
     1021 gcgtggacgt catcatgccc gacatcacct ttcttgccga acgccgcgac gaggtgctgg
     1081 gcatcgtcgc cacccacgcg cacgaggatc atatcggcgc gatctcttat ctgtggccga
     1141 cgctgcgctg tccggtctat gccacgccgt tcaccgccaa actgatcgaa ggcaagctcg
     1201 aggaagcggg attgaaaggg cgcgtgcgcc tccatgccgt gccgctgcaa agcaggctga
     1261 agctcggtcc gttcgagatc gacttcgttt cgctcaccca ttcgataccc gaacccaacg
     1321 cgctcgccat ccgcaccaag ctgggtgtcg tggtccacac cggcgactgg aagctcgatc
     1381 ccgaaccgct gatcggcgaa gccgcggacg cgggcgtgct gcgaaaactg ggcgacgaag
     1441 gcgtactggc gctggtctgc gattccacca atgcgctgct gccgggcgaa tccggctcgg
     1501 aagcgacggt gcgcaaggcg ctcaccgact tgatcggcac gctgaagggg cgggtggcgg
     1561 tcaccgcctt tgcctccaac gtggcgcggc tggacaccat cgccaaggcg gcgcgcctcc
     1621 acggccgcaa gctcgcgctg gtcggccgcg ccatgcacaa gatcgtcgaa gcggcgcgcg
     1681 ccacgggata tctcaaggac tttccgtcca ccgtggacga agaggaggcg gcggagctgc
     1741 cgccccgtcg cgtgctctat ctctgcaccg gcagccaggg cgagacccgc gcggcgctga
     1801 cccgcatcgc ctccggcgag caccagaacg tcaagctcgg tcccggcgat acggtgattt
     1861 tctcctcgcg catcatcccc ggcaacgacc tgcagatttt tgcgctgcat aacaaactgg
     1921 ccgctctggg cgtggaagtc ctttcggccg aggaccattt cgtgcacgtc tccggccatc
     1981 cggcgcaggg cgaactggcg cagatgtatc gctggatacg gcccaggatc gcggttccgg
     2041 tgcatggcga gttgcgccac atgagcgccc atgcgcgcct ggcgcgctcg ctccaggtgc
     2101 cgcaggcgat caatgtcgaa aacggtcatc tgctgcggct ggcgccgggc aaggcgcagg
     2161 tgatcgacga ggtgcccgcc ggccgcatcc atctggacgg acaggtgctg gtcgcggaag
     2221 gcgcgaggct gtcgtgcgca cgccgctcgc tgggctt
//
LOCUS       DASWPU010000410         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_46557,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000410 DASWPU010000000
VERSION     DASWPU010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46557"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VGC27_08830"
     CDS             complement(<1..594)
                     /locus_tag="VGC27_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282714.1"
                     /translation="MVTQPKTPSAALPQLATKTLSAIKAGVVTPAYDRRATDLGIVHF
                     GPGAFHHGHQSWYVDALLARDPRWAIADVELREGVLADAMAAQDCLYTVAQLDEQLQF
                     QVIGSTKEYIVASKDPEAAFSRLVTPSVRLVTMTVSEKGYCLDGSGALDLGHSDIKHD
                     LEHPVSPISVVGWVTQGLVRRRAAGLAPFVVTSCDNMV"
     gene            complement(750..2048)
                     /locus_tag="VGC27_08835"
     CDS             complement(750..2048)
                     /locus_tag="VGC27_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0282715.1"
                     /translation="MDKTLNFPDIRPYLRTFAAAALLMGLAVCGAAHAGRSLQPAAWV
                     GSWASSQQIPEPHNALAAEDLRDATLRQIVRLTIGGQDVRVRLSNAFGTEPLHIASVH
                     IARPLSPSAGSVDISTDKALTFSGSPDVTIPAGADYISDPVAFPVAALSDLAISLYLE
                     TPPAQQTSHPGSRQTSFLVHGNQVSAADLPNAKQVDHWYQISGVEVSAPPQAASVVIL
                     GDSITDGRGSTTNGNDRWPDNLARRFQASPATRLLGVLNAGIGGNRLLQDGLGPNALA
                     RFNRDVLAQSGVRALIVFEGINDLGTLTADHEVSAEEHQSLVRRMTGAYQQIVTQAHA
                     HGIRVMGATITPDMGNDGYHPTPLNEADRQAVNAWIRTPGHFDAIVDFDKAVRDPANP
                     DRLLPAYDSGDHLHLSPAGYRAMADAVPLSFFLRADRISR"
     gene            complement(2182..>2648)
                     /locus_tag="VGC27_08840"
     CDS             complement(2182..>2648)
                     /locus_tag="VGC27_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282716.1"
                     /translation="FLLSDYGLEGFGWTANATWISTKTTPNSAAPSVVLGVSPMTYNV
                     TGYYDNNGVMVKLSYAYQRGTVVGSNAYGIIANMAFIAEERSLDYAQLDLSSSIKLSK
                     FFGELPTDPDLTFDVQNLTHALNGRSYKQYPNILNYSYNAGSLFLIGIRGTF"
BASE COUNT          513 a          783 c          878 g          474 t
ORIGIN      
        1 gaccatgttg tcgcaactgg tgacgacaaa aggcgccagc ccggcggcgc gccggcggac
       61 cagcccttgg gtaacccagc cgaccacgct gatgggcgac accggatgct ccagatcgtg
      121 tttgatgtcg ctgtggccta gatcgagcgc tccggagccg tcgaggcagt agcccttttc
      181 gctgacggtc atcgtcacca gacggacgga cggcgtgacc aggcgggaaa acgcggcctc
      241 cggatccttc gaggcgacga tatattcctt ggtcgaaccg atgacttgaa actggagctg
      301 ctcgtccagc tgggccacgg tataaaggca gtcctgcgcc gccatggcgt ccgccaggac
      361 gccttcacgc agctccacgt cggcgatggc ccagcgcggg tcgcgcgcca gcagcgcatc
      421 gacgtaccag gactgatggc cgtgatgaaa cgcgccgggt ccgaaatgga cgatgccgag
      481 atcggtagcg cggcggtcgt aggctggcgt gacgacgccc gccttgatgg cggagagggt
      541 cttcgtcgcg agctgcggca aagcggcgga aggcgttttc ggctgtgtga ccaagatcgt
      601 tgtcctaacg tttctagtgt tatgaagcgg aggcgccacg ctcatcgcag aagaccaagc
      661 ggggcgcccg gcaagtatgg catttcgctt ccagaaggcc ctgcgtagcc atcggccgtt
      721 accgggcctt tggcgctgcg cctcttgcct tagcgcgaaa tcctatcggc acggagaaag
      781 aatgacaacg ggacggcatc ggccatagcg cggtaccccg cgggtgacag atgcaaatgg
      841 tcgccggaat cgtaggccgg cagcagacgg tcggggtttg cggggtcgcg caccgccttg
      901 tcgaaatcga caatggcgtc gaaatgtccc ggcgtcctga tccacgcatt gaccgcctgg
      961 cggtcggcct cattgagcgg cgtcggatga tagccgtcat tacccatatc aggggtgata
     1021 gtcgcgccca tcacacggat gccgtgtgcg tgtgcctgcg tcacgatctg ctggtaagcg
     1081 ccggtcatgc gccgcaccag cgattgatgt tcttccgccg atacttcgtg atcggccgtc
     1141 aaggtgccca aatcgttgat gccctcgaaa acgatcaggg cgcgcacgcc gctctgggcc
     1201 agcacgtcgc gattgaaccg cgccaaggca ttgggtccga ggccgtcctg cagaaggcgg
     1261 tttccgccta tgcccgcgtt gaggacgccg agcagccgcg tcgccggcga agcctggaac
     1321 cgccgggcca ggttgtcggg ccagcgatcg ttgccgttcg tcgtggagcc gcgcccgtcg
     1381 gtgatcgagt ctcccaggat gacaaccgaa gctgcttgcg gcggcgccga cacctccacg
     1441 ccggaaatct ggtaccagtg atccacctgt ttcgcgttgg gcagatcggc ggcggaaacc
     1501 tggttgccgt gaaccaggaa ggaggtctgg cgggaaccgg ggtggctggt ctgttgcgcc
     1561 ggcggcgtct ccagatagag cgaaatcgcc aggtcggaga gcgcggcaac cggaaaggcg
     1621 acgggatcgg aaatataatc ggcgccggcg ggtatggtga cgtcggggct tcccgagaag
     1681 gtcagcgcct tatccgtcga gatatccacg ctgccggccg acggcgacag cggccgggcg
     1741 atatgaaccg aggcaatgtg cagcggttcg gttccgaacg cattcgacag gcgcacccgc
     1801 acgtcttggc cgccaatcgt aaggcgcacg atctggcgca gcgtggcgtc gcgaaggtct
     1861 tccgcggcga gggcgttgtg gggttcggga atctgctggg aggacgccca tgatcccacc
     1921 cacgccgcag gctgcaagga gcgtccggcg tgcgcggcgc cacagaccgc caagcccatc
     1981 aggagagccg cggcggcgaa tgttcttaaa tacggccgga tatccggaaa gttcagggtc
     2041 ttgtccatgc gagccggccg aaaaagaaag gagcggcaca cattttcgta tgccgctcca
     2101 gaattaacaa taggacttcg cttcttttcc catcacccct tccgaaaagg caagaagggg
     2161 tgatggaaac aatgtcgcgg attagaacgt tccgcggata ccgatcagga acaacgaacc
     2221 cgcattatac gagtagttga gaatattcgg gtactgcttg tacgaccggc cgttcaacgc
     2281 gtgcgtcagg ttctgcacgt cgaacgtcag gtccggatcc gtcgggagtt cgccgaagaa
     2341 cttggacaac ttgatactcg acgacaggtc gagctgagcg tagtccagcg aacgctcttc
     2401 ggcgatgaac gccatgttgg cgatgatgcc gtacgcgttc gagccgacga ccgtgccgcg
     2461 ctgatacgcg taggacagct tcaccatgac gccgttgttg tcgtagtagc cggtaacgtt
     2521 gtaggtcatc ggcgacacgc ctagcaccac ggaaggtgcc gcgctgttgg gcgtggtctt
     2581 ggtgctgatc caggtggcat tcgccgtcca gccgaagcct tcaagcccat agtcactgag
     2641 caggaagt
//
LOCUS       DASWPU010000411         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_27692,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000411 DASWPU010000000
VERSION     DASWPU010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27692"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..>2983
                     /gene="ileS"
                     /locus_tag="VGC27_08845"
     CDS             154..>2983
                     /gene="ileS"
                     /locus_tag="VGC27_08845"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY0282717.1"
                     /translation="MSDKSEEKSAKDYRATLFLPHTEFPMKAGLPEAEPKWLARWEET
                     GLYARIREAAKGKPLFILHDGPPYANGEIHSGTGLNKILKDFVIRSQGMMGKDAPYVP
                     GWDCHGLPIEWKIEEKYRAAGKSKDDVPIDRLRRDCREFAARWIGVQREQFKRLGCMG
                     AWENPYTTMNYRAEATIVSELHKFVSNGLLYRGFRPVMWSPVEKTALAEAEVEYQEKT
                     SPTIYVKFPVVRHTGLTGAEVLEGASIVIWTTTPWTIPGNRAIAYSESMLYGLYEVTS
                     VGEGALASVGEKLVLALELAEQTAKHAKAELKFIVGVIPHGLACAHPFRGRGYDFEVP
                     VLPGEHVTADTGTGFVHTAPGHGEDDFDLMLALDKDYPAKNPDAFAIITAEGSFAANV
                     PLFAGKHILSRDGKKDGDANGAVIKELVDSGKLLAKGTLRHSYPHSWRSKAPVIFRAT
                     PQWFAAMDREFKGSNGKTLREIAMQAIRDTKWYPKAGENRIGSMVEGRPDWVLSRQRA
                     WGVPLAIFVDKKTGKILDDAEVNARIVEAFEKEGADAWFQPGSAARFLGPDRNPDDYE
                     QVRDILDVWFDSGSTHVFTVENPIDPAWPKKDNADLYLEGSDQHRGWFQSSLLESCGT
                     RGRAPYDAVLTHGFVLDEQGRKMSKSLGNTVLPQTIAEKNGADILRLWVASSDFTEDL
                     RIGQDIIKTNVDAYRRLRNTIRFMLANLAGFSDAERIGPSEMPELERYMLAKLAELDA
                     LVRHGYGEYDFNRVFGALFNFCTNELSAFYFDIRKDSLYCDPLDAPRRRAARTVIDEI
                     FRRVVTWLAPILCFTMEEAWTLRFPDGSVHLQSFPAAPAEWQAPALIEKWNRIRSLRR
                     VVTGALEIKRRDKIIGASLEAAPVLYVSDASDAQMLNEIDFAEVAITSAAHVVTREPP
                     PDAFTLPEVPGVAVTFHHADGDKCARCWM"
BASE COUNT          560 a          965 c          923 g          535 t
ORIGIN      
        1 tttcggcgga tttgcgcgaa ttcggtgccc ggcttcgctg gctgggggct agagccgcct
       61 gccggacgcg aaggccgggc ttgccgcgcc ggagcttcag cgaaggcggg atttgcttcc
      121 tgaaattctc ttaccgatcc aaggcctgaa tttatgtccg acaaatccga agaaaaaagc
      181 gcgaaggatt accgcgccac tttgttcctg ccccataccg agtttccgat gaaggcgggc
      241 ttgcccgaag ccgagccgaa atggctggcg cgctgggaag agaccgggct ctacgcccgc
      301 atccgcgaag ccgccaaggg caagcctctg ttcatcctgc atgacggccc gccctacgcc
      361 aacggcgaga ttcattccgg caccggcctc aacaagatcc tcaaggactt cgtgatccgc
      421 agccaaggca tgatgggcaa ggacgcgccc tatgtgcccg gctgggactg tcacggcctg
      481 cccatcgaat ggaagatcga ggagaaatat cgcgcggcgg gcaagtcgaa ggacgacgtg
      541 cccatcgacc ggttgcgccg cgactgccgc gaattcgccg cgcgctggat cggcgtgcag
      601 cgcgagcagt tcaagcggct cggctgcatg ggcgcgtggg agaatcccta cacgaccatg
      661 aattatcgcg ccgaggcgac catcgtttcc gagctgcaca agttcgtgag caacggcttg
      721 ctctatcgcg gcttccgccc ggtgatgtgg tcgccggtgg aaaagaccgc gctggccgaa
      781 gccgaggtcg agtaccagga aaaaacctcg ccgacgatct atgtgaagtt cccggtggtg
      841 agacacactg ggctgacggg agcggaagtt cttgaaggcg cttcgattgt catctggacc
      901 acgaccccgt ggacgatccc gggcaatcgc gccatcgcct attcggagtc gatgctttac
      961 gggctttatg aagtcaccag cgtcggcgag ggcgcgttgg caagcgtcgg cgagaagctg
     1021 gtcctcgctc ttgaacttgc ggagcaaacc gcgaaacacg ccaaggccga actgaaattc
     1081 attgtcggcg tcattcctca tggcttggct tgcgcccatc cgttccgcgg ccggggctac
     1141 gattttgagg tgcccgtgct tcccggcgag cacgtcaccg ccgacactgg caccggcttc
     1201 gtccacaccg cgcctggcca cggcgaagac gatttcgatc tgatgctggc gctcgacaag
     1261 gattatccgg cgaaaaaccc ggatgccttc gccatcatca ccgccgaagg ctcgttcgcg
     1321 gcgaatgttc cgctcttcgc cggcaagcac atcctgtcgc gcgacggcaa gaaggacggc
     1381 gacgccaacg gcgccgtgat caaggaactg gtcgactccg gcaaactgct ggcgaaaggc
     1441 acgctgcgcc actcttatcc ccattcctgg cgctcgaagg cgccggtgat cttccgcgcc
     1501 acgccgcaat ggttcgcggc gatggaccgg gagttcaaag gctcgaacgg caagaccttg
     1561 cgcgaaatcg cgatgcaggc gatccgcgac accaaatggt atcccaaggc aggcgagaac
     1621 cgcatcggct cgatggtgga aggccggccc gactgggtgc tgtcgcgcca gcgcgcctgg
     1681 ggcgtgccgc tcgccatctt cgtcgataag aagaccggca agatcctcga cgatgccgag
     1741 gtcaatgccc gtatcgtcga agcctttgag aaagagggcg ccgacgcctg gttccagccc
     1801 ggctccgccg cgcgctttct tggaccggac cgcaatcccg atgattacga gcaggtgcgc
     1861 gacatcctcg acgtctggtt cgattccggt tccactcacg tcttcaccgt ggaaaacccc
     1921 atcgacccgg catggccaaa gaaggacaat gccgacctct atctcgaagg ctctgatcag
     1981 catcgcggct ggttccagtc ttcgctgctg gaaagctgcg gcacgcgcgg ccgcgcgcct
     2041 tacgatgcgg tgctgaccca cggcttcgtg ctcgacgagc agggccgcaa aatgtcgaag
     2101 tcgctcggca acacggtgct gccgcagacc atcgccgaga aaaacggcgc cgatatcttg
     2161 cgcctgtggg tcgcctcctc ggatttcacc gaagaccttc gcatcggcca ggacatcatc
     2221 aagaccaacg tcgatgccta tcgccggctg cgcaacacca tccgcttcat gctggcgaat
     2281 ctggcgggct tctcggacgc cgaacgcatc gggccgagcg agatgcccga actcgaacgc
     2341 tacatgctgg cgaagctggc ggagctggac gcgctggtgc gccacggcta tggcgaatat
     2401 gatttcaatc gcgtcttcgg cgcgctgttc aatttctgca ccaacgagct gtcggcgttc
     2461 tatttcgaca tccgcaagga ttcgctttac tgcgatccgc tcgatgcgcc gcgccgccgc
     2521 gccgcccgca ccgttattga cgagatcttc cgccgcgtgg tcacctggct ggcgccgatc
     2581 ctttgcttca ccatggagga ggcgtggacc ttgcgcttcc cggacggcag cgtgcatctg
     2641 caaagcttcc ccgcggcgcc cgccgaatgg caggcgcccg cgctgatcga gaagtggaac
     2701 cgtatccgca gcttgcgccg ggtcgtcacc ggcgcgctgg agatcaagcg ccgcgacaag
     2761 atcatcggcg ccagcctgga ggctgcgccg gttctctatg tctccgatgc gtcggatgcg
     2821 cagatgctga acgagatcga tttcgcggag gtcgccatca cctcggccgc ccatgtggtg
     2881 acccgcgagc cgccgccgga cgccttcact ctgcccgaag tgccgggcgt ggccgtcacc
     2941 ttccatcacg ccgacggcga caaatgcgcg cgctgctgga tga
//
LOCUS       DASWPU010000412         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_34451,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000412 DASWPU010000000
VERSION     DASWPU010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34451"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..560
                     /locus_tag="VGC27_08850"
     CDS             144..560
                     /locus_tag="VGC27_08850"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEY0282718.1"
                     /translation="MSRILLAALMALMPLAASAATLTIKVENISRKGGVLRLSLYGEA
                     SWPDDKSEPIVSANVPALAPETVITFSSLKPGVYGVKTYQDANGNEKFDQNWLGWPLE
                     RYGFSRDARPFLSAPGFSRTKFTLPEGETAIVINLQ"
     gene            614..751
                     /locus_tag="VGC27_08855"
     CDS             614..751
                     /locus_tag="VGC27_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282719.1"
                     /translation="MIPRLETERLILRECRPQEIVIGGKTVTVAIRSITRAEGRRRQD
                     A"
     gene            748..1992
                     /locus_tag="VGC27_08860"
     CDS             748..1992
                     /locus_tag="VGC27_08860"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0282720.1"
                     /translation="MSDSSSPVLYRHRDLAFFLSGRFLGTVAMQVQSVAIGWQVYEIE
                     RTPLALGLVGLCQFLPMFLLTLPAGEITDRLNQRVVLAAAMVLQGVCGALFLGLSLAH
                     VETAAAFYAVLVLFGAARGFSGPAGQSLLAFLVPPERLARSIAVNSSIFMVAVIAGPA
                     LGGFLYALGPVATYSVCVVGFLATSVLTGTLGGRRVTKAVAADAGRLARVVEGIRFVR
                     TRPVVFGAISLDLFAVLLGGATALLPVFARDILFVGPIGLGLLRSAPAVGAAMIALAL
                     AGRPIERGTGWKMFAAVAVFGVATIVFGLSRNFTLSLCALFVLGASDMVSVFIRQALV
                     NFATPDEMRGRVGSVNVLFIGASNELGELESGLMAAWFGTIRAVVIGGLGTLAVVAAW
                     MKLFPPLRTVDRLSDVAPGHQL"
     gene            complement(1980..2543)
                     /locus_tag="VGC27_08865"
     CDS             complement(1980..2543)
                     /locus_tag="VGC27_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0282721.1"
                     /translation="MQRHILACALSAATLCALPARASSNMETAGTYVAIALPLAAGGI
                     AVWQDDWTGVKQLAVVGALTVGTAYGLKHLVRECRPFAHPCGPGGDNWDSFPSDTSAL
                     AFAPAQFLWQRYGWQYGVPAYAAAAFVGYSRVDAQKHHFWDVLTSAAISLAYNELITT
                     RYRPHKSLYTAIEASPDGVHMSLNYNW"
     gene            complement(2688..>3516)
                     /locus_tag="VGC27_08870"
     CDS             complement(2688..>3516)
                     /locus_tag="VGC27_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282722.1"
                     /translation="AQTVPGENVIVNPSAAGTRVLRYPGDKYVRIVPPLRQPGEATEP
                     IRLRMPASHRVVRSQAPREASAAPVAAPAPPKQTNTNRSNFDDLAVVRPAAPAPAPAR
                     PEPSLKPAPVRTPPPQRAASAEPPPQRAIRTEPQRTIRAEPPLQRMARTEPPPMRTPA
                     PASNPEADSGSKRGVVLFAPNATDPSVSAMQNIKTLAGSLSTTLGVGASRLQLLAFGG
                     PHGEKSSDSRRLSLRRALIVRQILIDNGVPSERIDVRAMGGTDDSGPLDRVDVFLKR"
BASE COUNT          553 a         1084 c         1216 g          663 t
ORIGIN      
        1 cgagcggatc atcgagctgg aactgacggc cgaggaaaaa gcggccttga acaagtccgc
       61 cgtgtccgta caggggctgc ttgaagtgtg caagaagatc gatcccaagc tggcttgaag
      121 caactcgtcc ggagagaacc actttgtcgc gcattcttct tgccgccctg atggcgctca
      181 tgccgctcgc cgcatccgcc gccacgctga cgatcaaagt cgagaacatc tccaggaagg
      241 gcggcgtgct gcgcctgtcg ttgtacggcg aagcctcgtg gccggacgat aaatccgaac
      301 cgatcgtcag tgccaatgtg cctgcgcttg cgcctgagac cgtgattacg ttttcgagtc
      361 tgaagcccgg cgtctacggc gtgaagacct atcaggacgc caacggcaac gaaaagttcg
      421 accagaactg gctgggttgg ccgctcgagc gctacggctt ttcccgcgac gcacggccgt
      481 ttctttccgc gcccggtttc agccgcacta aattcacctt gccggagggc gaaactgcga
      541 tcgtgatcaa tttacaatag acgcataccg gatccgaatt gtcgtgcgcg gacgaattat
      601 atagagaaga ggcatgatcc cgcgcctcga aaccgaacgt ctgatcctgc gcgaatgccg
      661 gccgcaggaa atcgtcatcg gcggcaaaac tgttacggtc gccatccgga gcatcacgcg
      721 cgccgagggg cggcgccggc aggacgcatg agcgactctt cttcgccggt tttatatcgt
      781 catcgcgatc ttgctttttt cctgagcggc cgcttcctcg ggaccgtggc gatgcaggtg
      841 caatccgtcg ccatcggctg gcaggtctac gagatcgagc gcacaccgct ggcgctcgga
      901 ctggtggggt tgtgccagtt cctgcccatg tttcttctca ctctgccggc gggcgaaatc
      961 accgaccgcc tgaaccagcg cgtggtgctg gcggcggcga tggtgctgca gggcgtgtgc
     1021 ggggcgttgt ttctgggtct cagcctggcg catgtcgaaa ccgccgccgc cttctacgcg
     1081 gtgttggtgc tattcggtgc ggcgcgcgga ttctccggtc cggccgggca atccctgctg
     1141 gcgttcctgg tgccgccgga acgtctggcg cgctcgatcg ccgtgaactc gtcgattttc
     1201 atggtcgccg tcatcgccgg acccgcgctc ggcggctttc tctacgcgct gggtccggtg
     1261 gcgacgtaca gcgtttgcgt cgtagggttt ctggccacca gtgtgctcac ggggacgctg
     1321 ggcgggcgcc gggtaacgaa ggccgtggcg gccgatgcgg gcaggctggc gcgcgtcgtc
     1381 gagggcatcc gtttcgtgcg cacgcgtccc gtcgtcttcg gcgcgatctc gctcgatctg
     1441 tttgcggtgc tgctgggcgg ggcgacggcg ttgctgccgg tgttcgcgcg cgacattctg
     1501 ttcgtcgggc cgattgggct tggcttgctg cgcagcgcgc cggccgtggg cgcggcgatg
     1561 attgcgctcg ccctggccgg tcgccccatc gagcgcggta ccggctggaa gatgttcgcg
     1621 gccgtcgccg tgttcggcgt cgccaccatc gtcttcggcc tgtcgcggaa cttcaccctg
     1681 tcgctgtgcg cgctgttcgt gctgggcgcc tccgacatgg tcagcgtctt catccgccag
     1741 gcgctggtca acttcgccac gcccgacgag atgcgcggcc gcgtcggctc ggtcaacgtg
     1801 ctgttcatcg gcgcctcgaa cgaattgggc gagttagaat ccggtctgat ggcggcctgg
     1861 ttcggcacca tccgggcggt ggtgatcggc gggcttggca cgctcgccgt ggtggcggcg
     1921 tggatgaagc tgttcccgcc gctgcgaacc gtcgaccggc tcagcgacgt ggctcccggt
     1981 caccagttgt aattcagcga catgtgaacg ccgtcgggcg aagcctcgat agcggtatag
     2041 agcgatttat gggggcgata tcttgtggtg atcagctcgt tataggctaa ggaaatcgcg
     2101 gcgctcgtca gcacgtccca gaaatggtgt ttctgggcgt cgacgcggga atagcccacg
     2161 aaggcggcgg cggcataggc cggcacaccg tactgccagc cgtagcgctg ccacagaaac
     2221 tgcgccgggg cgaaagccag tgcggaagtg tcggagggaa aggaatccca gttgtcgccg
     2281 cctgggccgc aaggatgcgc gaagggccgg cattcgcgca ccaggtgttt cagcccatag
     2341 gccgttccga cggtgagggc gccgacgacg gcaagctgct tcaccccggt ccaatcgtcc
     2401 tgccacaccg caatgccgcc cgccgcaagc ggcagagcga tggcgacgta ggttccggcg
     2461 gtttccatgt tggaggaggc gcgagcaggc agcgcgcaaa gcgtggcagc gctcagcgcg
     2521 caggcgagaa tatgacgttg caaagtgcac catccccata tgacgatttc atgaacctac
     2581 agtgcatccg gcgccggcgc aatcgagccc gctgccctga tttaccaagg acgtggcatt
     2641 cacgcgagat taggagggcg caacggcctt cgatccgcag cgccctctca ccttttcagg
     2701 aagacatcca ctcggtcgag cgggccgctg tcgtccgtgc cgcccatggc gcgcacgtcg
     2761 atgcgttcgg acggcacgcc gttgtcgatc agaatctggc ggacgatgag tgcgcgcctg
     2821 agcgacagcc gccgcgaatc cgaggatttt tcgccgtgcg gaccgccgaa ggccagaagc
     2881 tggaggcgcg aagcgcccac gccaagcgtc gtgctcaaac tgccggcgag cgttttaata
     2941 ttctgcattg ccgatacgga tggatccgta gcgttcggcg caaacagaac gacgccgcgt
     3001 ttggaaccgc tgtcggcttc gggattggac gccggcgccg gtgtgcgcat cggcgggggt
     3061 tcggtgcgcg ccatgcgttg aagcggcggt tcggcgcgta tcgtgcgctg cggctcggtg
     3121 cgtatcgcgc gctgcggcgg cggttcggcg ctggccgcgc gttgcggcgg cggagtacgc
     3181 accggcgcgg gcttgaggct tggttcgggc ctggccggcg cgggcgccgg agctgccggc
     3241 cggacgaccg cgaggtcgtc gaaattcgaa cggttggtgt tcgtctgctt aggcggcgcg
     3301 ggggcggcaa ccggcgcggc agacgcttcg cgcggagcct ggctgcgtac aacacggtga
     3361 ctggcgggca tgcgcaggcg gatcggctcg gtggcttcgc ccggctggcg caaaggcgga
     3421 acgatgcgca catatttgtc gcccggatag cgcaacaccc gggtacccgc ggcagagggg
     3481 ttcacgataa cgttctcgcc ggggaccgtc tgcgcc
//
LOCUS       DASWPU010000413         9376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_15800,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000413 DASWPU010000000
VERSION     DASWPU010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9376
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15800"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="VGC27_08875"
     CDS             complement(<1..1641)
                     /locus_tag="VGC27_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY0282723.1"
                     /translation="MAQSGAEFAEQLEYAAAGRAKSRSLAPLRGLLPFLKPYRWTILA
                     AIVAMLVAATATLVLPLAGRGLIDHGFSAAKAAAISRYFLAFLAVAAVMGISSAVRYY
                     FVTWIGERVIADLRKAVFDNVISLTPSFFEMTRTGEVLSRLTADTTLIQTVIGSSVSV
                     AARNAVTLAGGLVMMFVTSLKLSALVVGAVALVMLPLMLFGRWVRSLSRKSQDKIADT
                     SAHASETINAVQTVQAFTHEKYERSFFGAAVEDSFGMAVLRTRARAVMTGVVIFAVFA
                     SIVCVGWVGAQDMIAQRLTGGLLLQFIIYAILVAGGVGALSETWGDIQRAAGASERLM
                     ELLHAVPAIAAPARPARLPEPARGAVKFEHVRFHYPSRPDAAALEDFSFSVAPGEAVA
                     LVGPSGAGKSTVFQLLLRFYAAQEGRISFDGVDIAALDPAELRRHIAIVAQDSVIFSG
                     TLADNIRYGRPDASEEDVRRAAEAAAAGEFIARLPDRLATRVGERGVTMSGGQRQRVA
                     IARAILRDAPLLLLDEATSALDAENERLVQQGFANLIAG"
     gene            1768..1989
                     /gene="rpmE"
                     /locus_tag="VGC27_08880"
     CDS             1768..1989
                     /gene="rpmE"
                     /locus_tag="VGC27_08880"
                     /inference="COORDINATES: protein motif:HMM:NF001809.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY0282724.1"
                     /translation="MKKGIHPDYHFVNVHLNDGTSYRTRTTWGTPDQKLTLDIDPTTH
                     PAWTGGQHQLLDRGGRLTRFNKKFKDFVK"
     gene            complement(2069..2578)
                     /locus_tag="VGC27_08885"
     CDS             complement(2069..2578)
                     /locus_tag="VGC27_08885"
                     /inference="COORDINATES: protein motif:HMM:NF018972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEY0282725.1"
                     /translation="MAFIDVGDDNLHELTLQSFTGSALFQRTFDEGMSLVEETARYLD
                     GPGREEQRKLPRKTAMLYAGGSMRVTTRLMQAASWLLVQRAVHDGDMDVEDASSDRYR
                     LGSKEICFGGSAEGVELLPAMLQDLLSRSDNLYRRIARLDEVLFDNGLMPAGARGRFA
                     LLEEAFGGK"
     gene            complement(2730..2927)
                     /locus_tag="VGC27_08890"
     CDS             complement(2730..2927)
                     /locus_tag="VGC27_08890"
                     /inference="COORDINATES: protein motif:HMM:NF018413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEY0282726.1"
                     /translation="MAIDPEELLPRKKPSGITLGEDITMLSEFELSARIMALEEEIAR
                     CRQAIGVRQATKSAADSFFKR"
     gene            2995..3981
                     /locus_tag="VGC27_08895"
     CDS             2995..3981
                     /locus_tag="VGC27_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY0282727.1"
                     /translation="MPTMKAIAVVNPGPVLSLGETKKPQAGPGEILVKVAAAGVNRAD
                     LLQAMGLYPPPPGAPLTLGLEISGEIAELGAGVVNHKLRDRICALVTGGGYAEYAVAA
                     QECVLPVPKGLDLLEAAALPEAYFTVWTNLIDTARLKPGESVLIHGGSSGIGVTAIQL
                     CAACGQTVFATAGSDRKCAICETLGARRAVNYHSGDFVKAVLEETDGKGVDVILDMVG
                     GDYIGRNFAAAAKGGRIVNIAYQAGATAVVNFTPMLLKRLTLTATTLRPRLNAEKGAI
                     RDALKREVWPMIEAGKIKPVIDRVFPLAEAQAAHARMAKGEHIGKILLKVPA"
     gene            4186..4881
                     /locus_tag="VGC27_08900"
     CDS             4186..4881
                     /locus_tag="VGC27_08900"
                     /inference="COORDINATES: protein motif:HMM:NF018001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEY0282728.1"
                     /translation="MAVENKPLMPKATAVWLVDNTSLTFEQIADFCGLHPLEVKGIAD
                     EDVAKGIKGQDPVTSGQLSREQIVEAENNHKLRLKMAPPKYKMPVVKQKKAPRYTPVS
                     KRQDKPDAVYWILRNHPEFTDADIIKLVGTTKATIQKIRERSHWNATNIKAVDPVTLG
                     LCSQLELDLAVSRAALKRERAIKRAAKRGETLKPLDDAPGGAQPEAQTEEQTEAPVIA
                     EDSNKPEDETSEP"
     gene            complement(4888..6132)
                     /locus_tag="VGC27_08905"
     CDS             complement(4888..6132)
                     /locus_tag="VGC27_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0282729.1"
                     /translation="MIPQLTSITAILSTTLIFLVGNGLLGTLIPVRAHLEGFSTLAIG
                     VIGSVYFAGFVLGCFTGPRWMARVGHIRTFAVCAGLAAAATLVQSIFVSVAAWIAMRA
                     LFGFAAANMYMVLESWLNDRADNKTRGSIFASYLSVNFGGLLIGQLLFATARPESFVM
                     FSLATIFYALCLIPVGLTALKQPAPAPVPVLRPLRLFRISPVGVAGCIAVGLANNAIW
                     TLAPVYAQSRGLSSGWIALFMCAFTIGGTLVQVPVGRLSDRMDRRYVIAAISALAAAA
                     GIAIALFMGSGRGAMLWAIAGFGVLVLPLYGLSVAHANDRIPRANFVESSATLLLINS
                     LASVAGPTIAASVMDMFGAQSLFFFTAAVHVAMTGFTVVRLRQKEAPGGEYREKFAPL
                     PHEASPSSLELDPRGPEEGKAA"
     gene            complement(6230..6847)
                     /locus_tag="VGC27_08910"
     CDS             complement(6230..6847)
                     /locus_tag="VGC27_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEY0282730.1"
                     /translation="MIDEKIAARRGGLLRAMYAWVMRHSVGPHRYAVLAAVAFTEASF
                     FPIPPDLFLVPMVLADRKRAFLLAGVCTLGSVIGGLFGYAIGALLYDSVGHWLIQVYG
                     YGQDLAAFRDAYDKWGAWIILIKGLTPIPYKLVTIASGFAGYNLALFMVLSVITRGLR
                     FAIVAGLLYWFGEPVREFIEKRLEYVLFGFLIVIVLGFVIARYVV"
     gene            complement(7088..7384)
                     /locus_tag="VGC27_08915"
     CDS             complement(7088..7384)
                     /locus_tag="VGC27_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282731.1"
                     /translation="MFSVVPSVSTPVKNNTCRQLDAAGAEQIAPAHDMVAKSAARFFS
                     KTLRCAPQSMRAMPVGVKIEFEVKRPAPVQRSIAGEHVFVHRVPVRPFYYRRRE"
     gene            7390..7950
                     /locus_tag="VGC27_08920"
     CDS             7390..7950
                     /locus_tag="VGC27_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282732.1"
                     /translation="MSAGAADYNKIAKEKNWVSLCDASVTFSHPDLRRLLGLWRKQAK
                     GGIPLYRNMTSRVLKSFLTDIAICERIVGDGGERRYRICMMGVWLAQILGNFTGKFLD
                     DVLPPEHLPRWQVALDATLADRVPLRFLGRTDTNAMSFLNGEYFTAPLLADDGSASFI
                     LAAGRFTGGRSWEEVEAEARQALGLL"
     gene            complement(8120..8293)
                     /locus_tag="VGC27_08925"
     CDS             complement(8120..8293)
                     /locus_tag="VGC27_08925"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY0282733.1"
                     /translation="MALEGHIQELSEKHRKLEELIETEMAHPDWNEARVAALKKQKLQ
                     IKDELERLRSSVH"
     gene            8397..9071
                     /locus_tag="VGC27_08930"
     CDS             8397..9071
                     /locus_tag="VGC27_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEY0282734.1"
                     /translation="MGKNSTVTSVRERLIVGISGASGVAYGIRLLEALAELGVESHLV
                     ISKAGQLTIGYETSLTPKAVASKADFSYPVGDIAAPIASGSFRTKGMIVAPCSVRTMS
                     EIATGVTTTLLTRAADVALKERRTLVLMVRETPLHLGHLRTMAALAEMGAVLLPPVPA
                     FYTAPRTLEDLVDHTVARALDAFGYDWPKAKRWGEGSAGAFSGEVCSGSPQKMRPKSS
                     TRSKKA"
     gene            9073..9285
                     /locus_tag="VGC27_08935"
     CDS             9073..9285
                     /locus_tag="VGC27_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY0282735.1"
                     /translation="MTGEVTAPDEVALRAKLAELTQEHRDLDAAIDALSSTRDQLQLT
                     RLKKRKLQLKDQIQKINDQLLPDIIA"
BASE COUNT         1900 a         3025 c         2888 g         1563 t
ORIGIN      
        1 gccggcaatc aggttggcaa agccttgttg cacaagcctc tcgttctcgg cgtcgagtgc
       61 gctggtggct tcgtcgagca ggagcagcgg cgcgtcgcgc aggatggcgc gggcgatggc
      121 gacgcgctgg cgctggccgc ccgacatggt gacgcctcgt tcgccgacgc gggtggcgag
      181 cctgtccggc aggcgcgcga tgaattcgcc cgcggcggcg gcctcggcgg cccgccgcac
      241 atcttcctcg ctcgcgtccg ggcgcccgta gcggatattg tcggcaaggg tgccggaaaa
      301 aatcaccgaa tcctgcgcca caatggcgat atggcggcgc agttcggccg gatcgagagc
      361 ggcgatgtca acgccgtcga aggaaatcct tccttcctgg gcggcataga agcgcagcag
      421 aagctggaac acggttgact tgccggcgcc cgaaggcccc accagcgcca ccgcttcgcc
      481 cggcgccacg gaaaacgaga aatcctccag cgctgcggca tccggccgcg aagggtagtg
      541 gaagcggaca tgctcaaact tgaccgcgcc ccgcgccggc tccggcagcc gagcgggacg
      601 ggcgggcgcg gcaatggcgg gcacggcgtg cagcagttcc atcaagcgtt ccgaggcgcc
      661 ggcggcgcgc tggatgtcgc cccaggtttc gctgagtgcg cccaccccgc ccgccaccag
      721 gatggcgtag atgatgaatt gcagcagcag cccgccggtc aaccgctgcg cgatcatgtc
      781 ctgcgcgccg acccagccga cgcagacgat cgacgcgaac accgcgaaaa tcacgacccc
      841 cgtcatcacc gcgcgcgcgc gggtgcgcag gaccgccatg ccgaaggaat cttccaccgc
      901 cgcgccgaag aagctgcgtt cgtatttttc gtgcgtgaag gcctgcacgg tctgcaccgc
      961 gttgatggtt tcgctggcat gggcgctggt atcggcgatc ttgtcctggc tcttgcggct
     1021 gaggctgcgc acccagcgcc cgaacagcat cagcggcagc atcaccagcg ccaccgcacc
     1081 caccaccagc gccgaaagct tgaggctggt gacgaacatc atcaccagcc cgccggccag
     1141 cgtcacggcg ttgcgcgccg ccaccgacac ggaagagccg atcacggtct ggatcagagt
     1201 cgtgtcggcg gtcagccgcg acaggacctc gccggtccgc gtcatttcga agaaggaggg
     1261 cgtgagcgag atgacgttgt cgaacaccgc cttgcgcagg tcagcgatga cgcgctcgcc
     1321 gatccaggtc acgaaatagt agcgcaccgc cgaagagatc cccatcaccg ccgcgacggc
     1381 caggaacgcc aggaagtagc gcgagatggc ggcagccttc gccgcgctga aaccgtgatc
     1441 gatcagcccg cgcccggcca gcggcaggac aagagttgcc gtcgccgcca ccagcatcgc
     1501 cacgattgcg gcaaggatgg tccagcgata gggcttgagg aacggcagca ggccgcgcaa
     1561 aggagccagg gagcgggatt tggcccgtcc ggcggcggca tattccaact gttcggcgaa
     1621 ttccgcgccc gattgcgcca tattttgccc gcttttccgc gaatgccccc cttatatagg
     1681 agcggccggg gcggcgcgaa gggcgtgctt gaaccttccg ggccgatccc ctataagccg
     1741 cgccttgctg aaaaagggcc ggccgccatg aaaaagggca ttcaccccga ctatcacttc
     1801 gtgaacgttc acctgaacga cggcacgagc tatcgcacgc gcaccacctg gggcacgccg
     1861 gaccagaagc tgacgctcga catcgacccg accactcacc cggcctggac cggcggccag
     1921 catcagctgc tcgaccgcgg cggccgcctg acgcgcttca acaagaagtt caaggatttc
     1981 gtgaagtagc gaagggctga ataagcctgg atccccttcc ctcgcccagc cgaagctggg
     2041 ctcgccgggg atgacgagct gtcgctcgtc acttcccgcc gaaggcttct tccagcagcg
     2101 cgaagcggcc gcgcgcaccc gccggcatca agccattgtc gaacagcact tcatccagcc
     2161 gcgcgatccg gcgataaagg ttgtcgctgc gcgagagcag gtcttgcagc atcgcgggca
     2221 gaagctccac gccttcggcg ctgccgccga aacaaatctc cttcgagccc aggcgatagc
     2281 ggtcgctcga ggcgtcttcg acatccatat cgccgtcgtg cacggcgcgt tgaaccagca
     2341 gccagcttgc cgcctgcatc aggcgcgtgg tcacgcgcat gcttccgccg gcatagagca
     2401 ttgcggtctt gcgcggcagc ttgcgctgtt cctcgcggcc cgggccgtcg agataacgcg
     2461 cggtttcctc caccagcgac atgccttcgt cgaaggtgcg ttgaaagagc gccgaaccgg
     2521 tgaacgactg aagcgtcagc tcgtgcagat tatcgtcgcc gacatcaatg aacgccatac
     2581 gaatcccccg cccgcggttt atctcgtatc aagctatcaa ttcattcgcg gcgcgcaaga
     2641 tgcgacaatg cataaaccaa ggcgatggtg gctaagaggt cacgttcttc ttgttcatat
     2701 taatcattga cggactgcgc ccgcgaaatt caccttttga aaaacgagtc ggcggcggac
     2761 ttcgtcgcct ggcgcacgcc aatggcctgg cggcagcgcg cgatttcctc ttctagcgcc
     2821 atgatgcgcg cggataattc gaattccgac agcatggtga tgtcttcccc cagcgtgatg
     2881 ccggagggtt ttttgcgggg cagcaattct tcgggatcga tggccatcgt ttcggtcctg
     2941 gcgacgcaat gatagtttcc atccgcggca cgccgtcgcg caaggatcaa tgccatgcca
     3001 accatgaagg cgatcgcggt tgtaaatccc ggccccgtgc tgagccttgg agaaaccaag
     3061 aaaccgcagg ccggacctgg ggaaatcctg gtcaaagtgg cggcggcggg ggtgaatcgc
     3121 gccgacctgt tgcaagcgat ggggttgtat ccgcctccac ctggtgcacc gttaacgctg
     3181 gggctggaaa tctccggcga gatcgccgaa ctcggcgccg gcgtcgttaa tcacaagctg
     3241 agagatagga tatgcgcgct ggtgaccggc gggggctatg ccgagtatgc ggtggcggcg
     3301 caggaatgcg tgctgccggt gcccaagggc ttggacctgc tggaagccgc cgccttgccc
     3361 gaggcctatt tcaccgtctg gacgaatttg atcgacacgg cgcgcctcaa gccgggcgaa
     3421 agcgtgctga tccacggcgg ttccagcggc atcggcgtga ccgcgatcca gttgtgcgcc
     3481 gcctgcggcc aaacagtgtt cgccacagcg ggcagcgatc gaaaatgcgc catatgcgaa
     3541 accttgggcg ccaggcgggc ggtcaactat cacagcggcg atttcgtcaa agccgtgctg
     3601 gaggagacgg acggcaaagg cgtggacgtc atcctcgaca tggtgggggg cgattatatc
     3661 gggcgcaatt tcgcggccgc ggccaagggc gggcgcatcg tcaatatcgc ctatcaggcg
     3721 ggcgccacgg cggtggtgaa cttcaccccc atgctgctga agcgcctgac gctgacggcc
     3781 acgacgttgc gaccgcgctt gaatgcggag aaaggcgcga tccgcgacgc gctcaagcgc
     3841 gaagtgtggc cgatgatcga agccggaaaa atcaaaccgg tgatcgaccg cgtcttcccc
     3901 ctcgccgaag cccaagccgc acacgcacgg atggcgaaag gcgagcatat cggcaagatt
     3961 ttgctgaagg tgccagcctg atgccgatga ggctcaaacg gcgcatttcc tgagtaaaaa
     4021 agccatcgcc aagcgcgtat tgtggcgggg tttctgccgc ggcgtccgta aaaacccttc
     4081 ccctcccctc caaaccccgc taaaagcctc cccattcctc gaaaggttaa gccttgcgag
     4141 gtcgggtgga gcggtgaacg ctcccccggc aagggagaag atttcatggc cgtcgaaaac
     4201 aaaccgctga tgcccaaggc caccgccgtc tggctggtgg acaacacttc gctcaccttc
     4261 gaacagatcg ccgatttctg cggcctgcac ccgctcgaag tgaagggcat cgccgacgag
     4321 gatgtcgcca agggcatcaa gggtcaggac ccggtgactt ctggccagct cagccgcgag
     4381 cagatcgtgg aggccgagaa caaccacaag cttcgcctca agatggcgcc gccaaaatat
     4441 aagatgccgg tggtcaagca gaagaaggcg ccgcgctaca ccccggtctc caagcgccag
     4501 gacaagcccg acgcggtcta ttggatcctg cgcaaccatc ccgaattcac cgacgccgac
     4561 atcatcaagc tggtaggcac caccaaggcc accatccaga agatccgcga gcgctcgcat
     4621 tggaacgcca ccaacatcaa ggcggtcgat ccggtgacgc tgggcctgtg ttcgcagctc
     4681 gaactcgacc ttgcggtcag ccgcgccgcg ctcaagcgcg agcgggcgat caagcgcgcc
     4741 gccaagcggg gcgagacgct gaaaccgctg gatgacgcgc ccggcggggc tcagcccgaa
     4801 gcgcagaccg aggagcagac cgaagcgccg gtgatcgccg aggattccaa caagccggaa
     4861 gacgagacca gcgagccata aacggattca ggccgccttc ccttcttccg gcccgcgcgg
     4921 atcgagttcc agcgagctgg gcgaagcttc gtgcggcagg ggcgcgaact tctcgcgata
     4981 ctccccgccc ggcgcttcct tctggcgcag gcgaacgacg gtgaaccccg tcatcgccac
     5041 atgcaccgcc gccgtgaaaa aaaacaaact ctgtgcgccg aacatgtcca tgacgctggc
     5101 ggcgatcgtc ggtcccgcaa ccgaggccag cgaattgatc aacagcagcg tcgccgacga
     5161 ttccacgaag tttgcgcgcg ggatgcgatc gttggcgtgc gccaccgaca aaccatagag
     5221 cggcagcacc agaacgccga acccggcgat cgcccacagc atcgcgccgc gtccgctccc
     5281 catgaacagc gcgatggcga tgccggcggc ggcggccaag gcagagatcg cggcgatgac
     5341 gtagcgccgg tccatgcggt cggagagccg gcccacgggc acctgcacca gcgtgccgcc
     5401 gatggtgaag gcgcacatga agagagcgat ccagccgctg gagagcccgc ggctctgggc
     5461 gtagaccggc gccagagtcc agatcgcgtt gttggcgagt cccacggcga tgcagccggc
     5521 gaccccaacc ggagaaatgc ggaacagcct gagcggcctc agcaccggca ccggcgcggg
     5581 cgccggctgc ttgagcgccg tcagcccgac cggaatcaag cacagcgcat agaagatcgt
     5641 cgccaagctg aacatgacga agctctccgg ccgcgccgtc gcgaacagca actggccgat
     5701 cagcagcccg ccgaaattga cgctcagata ggaggcgaag atgctgccgc gcgttttgtt
     5761 gtcggcccgg tcgttgagcc agctttccag caccatatac atgttggccg cggcgaaacc
     5821 gaacagcgcg cgcatcgcga tccaggcggc gacactgacg aaaatggatt gcaccagcgt
     5881 cgcggccgcg gccagcccgg cgcacacggc gaaggtgcgg atgtggccga cgcgcgccat
     5941 ccagcgcggc ccggtgaagc agcccagcac gaacccggcg aaatagaccg agccgatgac
     6001 gccgatggcg agcgtggaga acccttccag atgcgcgcgc accgggatca atgtgccgag
     6061 caatccgttg ccgacgagga aaatcagcgt cgtggaaaga attgccgtga tggaagtgag
     6121 ttgggggatc atgcgtgtgg tattttcatg aagatggccg aaccatcagg ccgtcattct
     6181 actctggaac gggcggacgc cggcaaatcc aggtttgaac atgccgcctt cacaccacgt
     6241 agcgcgcaat cacaaacccc aggacgatca cgatcaggaa gccgaacaga acatattcaa
     6301 ggcgcttctc gatgaactcg cgcaccggtt cgccgaacca gtagagcagc cccgcgacga
     6361 tggcgaaacg cagcccgcgc gtgatcaccg acagcaccat aaacagcgcc agattgtagc
     6421 cggcgaagcc cgaggcgatg gtcaccagct tgtaggggat gggcgtaagt cccttgatga
     6481 ggataatcca ggcgccccat ttgtcatagg catcacggaa agcagcaaga tcctgcccgt
     6541 agccgtaaac ctgaatcagc caatgcccca ccgaatcgta cagcagcgcg ccgatggcat
     6601 aaccgaacag cccgccgatc accgatccca gcgtgcatac ccccgccagc aggaaggcgc
     6661 gcttgcggtc cgccagcacc atcggcacca gaaagagatc gggggggatg gggaagaacg
     6721 aggcctccgt gaaggccacg gccgccagca ccgcgtagcg gtgcggtccc accgaatggc
     6781 gcatcaccca ggcgtacatg gcgcgcaaaa gcccgccgcg ccgcgccgct atcttttcgt
     6841 caatcatccg ctccatcctt tggggcgcct tcctagcaac gcgcctatcc tgcgtccatg
     6901 cgccttggcg ctcggcagag gggacgcatg atgaatccga agctcctcat tgatggaaaa
     6961 cggattgcca aagtttccca atgacatgca tatagaccaa atcaacctgt ggttgttatc
     7021 ctgggcgggt tccgggccgt tccatgaagc gtcttgcgcc gtttacgctt cccgctctcg
     7081 ccgaatttca ttccctccgg cggtagtaaa agggccgcac tggaacgcga tgaacgaaga
     7141 cgtgctcgcc ggcaattgag cgctgaaccg gcgcgggcct tttaacctca aactcgattt
     7201 tcacccccac gggcatcgcc cgcatgcttt gtggcgcgca gcgtagggtc tttgagaaaa
     7261 agcgtgccgc gctttttgca accatgtcat gcgccggcgc gatttgctct gcgcccgccg
     7321 catccaattg gcgacaagta ttatttttca cgggcgtcga gacgctcgga acaacggaga
     7381 acatccacaa tgtctgccgg cgccgccgac tacaataaaa tagccaagga aaaaaactgg
     7441 gtatcgctct gtgacgcatc cgtcaccttc agtcatcccg atttgcgccg cctgctgggg
     7501 ttgtggcgca aacaggcaaa gggcggcatt cctctatacc gcaacatgac atcgcgcgtg
     7561 ctcaagagct ttctcacgga catcgcgatc tgcgaacgca ttgtgggcga cggcggcgaa
     7621 cggcgctacc gcatctgcat gatgggcgtc tggctcgccc agatcctggg caacttcacc
     7681 ggcaaattcc tggacgacgt gctgccgccg gaacatctgc cgcgctggca ggtggccctt
     7741 gacgccacgc tcgccgaccg cgttccgctg cgctttctcg gccgtaccga caccaatgcc
     7801 atgagcttcc tgaacggcga atatttcacc gcgccgttgc tcgccgacga cggctctgcg
     7861 agtttcatcc tggcggcggg ccggttcacc ggcgggcgtt cctgggagga agttgaagcc
     7921 gaggcgcgtc aagcgttagg cctgctataa gaaggatgaa cgaacggcgg ctctattttc
     7981 cgggcgcgcc cttcctcgcg gcggcggcca tcgccttcgc cggcgtcaaa ccggcgccgg
     8041 cggggagatc atctcatctt gcggaataaa acaccccttc gacgcctttc ggcgccgcgc
     8101 ttcggggcac gcaaacggat cagtggacgc tgcttcgcag cctttccaat tcgtccttga
     8161 tttgcagttt ttgtttcttg agggcagcta cccgggcttc gttccaatcg gggtgtgcca
     8221 tctcggtttc gataagttct tccagtttcc tatgcttttc ggacaactcc tggatatgcc
     8281 cttcaagtgc catgcacgca cctctttgtt ttaggcatag acgattagag caccaagtcg
     8341 cggttctgtc acggccctct tccgtctatg gcttaggacc tgtaaagggt tgagccatgg
     8401 gaaaaaattc gaccgtaacg agcgtccggg agcgtctgat cgttggaatc tccggcgctt
     8461 ccggcgtggc ttacggcatc cgcctgctgg aggcgctggc cgagctggga gtggaatctc
     8521 atctggtgat cagcaaggcc ggacagttaa cgatcggtta cgaaacctcc ttgacgccca
     8581 aggccgtagc gtcaaaggcg gatttttctt accccgtcgg cgatatcgct gcccccatcg
     8641 cctcgggaag tttccggacc aagggcatga tcgtggcgcc ctgctcggtc cgcaccatga
     8701 gcgagatcgc caccggcgtc acaaccacgc tcttgacccg cgccgccgac gtagcgctga
     8761 aggaacggcg taccctcgtg ctcatggtgc gcgagacgcc gctgcatctg ggccatctgc
     8821 ggaccatggc ggccctggcc gagatggggg cggtcctcct gccccccgtc cccgccttct
     8881 ataccgcgcc gaggaccctg gaggaccttg tcgatcacac ggttgcacgc gccctcgatg
     8941 ctttcgggta tgattggccc aaggccaagc gatggggcga aggctcggca ggagcatttt
     9001 caggcgaagt gtgcagcggt tcgccgcaga aaatgcgacc caaaagtagc acccgctcca
     9061 agaaggcgtg aaatgaccgg tgaagtcacg gccccggacg aagttgcgct gcgcgccaag
     9121 ctcgccgagc tgacgcagga gcatcgcgat ctcgatgcgg cgatcgacgc gctttccagc
     9181 acgcgcgatc aactgcaatt gacgcggctg aaaaagcgca agctgcagct caaggatcag
     9241 attcagaaaa tcaacgacca gctcttgccg gacatcattg cttagaacgc gatccgccca
     9301 aacaggatcg taccatccac atgcttcgac aggctcagca tgaaggttta gccagccccc
     9361 tcaccctgag cttgtc
//
LOCUS       DASWPU010000414         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_120019,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000414 DASWPU010000000
VERSION     DASWPU010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120019"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VGC27_08940"
     CDS             <1..166
                     /locus_tag="VGC27_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY0282736.1"
                     /translation="AVEQGGVTVLIDSTSLEFLKGAEIDFVDDLIGASFKVNNPNATS
                     SCGCGTSFSV"
     gene            341..1123
                     /locus_tag="VGC27_08945"
     CDS             341..1123
                     /locus_tag="VGC27_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0282737.1"
                     /translation="MKIATWNVNSVKARLEAATAWAKEAVPDVVCLQEIKCTDDTFPA
                     AAFEDLGYNSAVHGQKTYNGVAILSKRPLEDVTPRLPGGDGDEHARYLEALVPGGKGM
                     VRVASIYAPNGNPIGTEKFTYKLAWLERLYERARALLAFEEPAVLMGDYNVIPTSEDC
                     WDPAAWATDALFQPESRAALRRIENLGYTDAFRSGNPQPYQYTFWDYQAGAWQKDHGI
                     RIDHILLSPQALDRLKACAIDKRVRGREKPSDHVPVWCELDI"
     gene            1390..1836
                     /locus_tag="VGC27_08950"
     CDS             1390..1836
                     /locus_tag="VGC27_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282738.1"
                     /translation="MRAGAAFVFFLVALTTGIAAAAPAKLVYRIDRATATIAHNRFVI
                     VARGAVSTGGWEKPLLRLQKTSVSEASTLEVEFVATPPHPRAAVAHAILPVSARLAAP
                     LPRTGVVQVKIVSQTNSVIVPVTRLSGTSAQPHAVPAAAYLARQSN"
     gene            complement(1940..>2564)
                     /locus_tag="VGC27_08955"
     CDS             complement(1940..>2564)
                     /locus_tag="VGC27_08955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0282739.1"
                     /translation="VNDYLTRPVDKNELVARVRTQLRKKLYSDRLRHNVQLSLEMAIT
                     DQLTGLHNRRYMSRHLDNLLAQARKSGKPLAFVIMDIDFFKLVNDTHGHDIGDEVLRE
                     FSRRISANVRGIDLACRFGGEEFVVVMPDTDIAYAYSVAERLRKSIETTPIEISRAPG
                     KLTITISIGIAGSEGEGDGAEDLLHRADQALYRAKKTGRNRVVADAA"
BASE COUNT          474 a          829 c          778 g          483 t
ORIGIN      
        1 ggcggtcgag caaggcggcg tcaccgtgct gatcgactcc acttcgctgg aattcctcaa
       61 aggcgccgaa atcgatttcg tcgacgacct gatcggcgcc tcgttcaagg tcaacaaccc
      121 gaacgccacc tcctcctgcg gctgcggcac gagtttttcg gtttaacccc cttgaacctt
      181 gtcgctcgaa ttaccatttc tcgtaaatcc tgaaatctgc gctctgacgc cggccgcctt
      241 gcggcccggt ttcgcgcgga tttttcaaaa tccccggttc gatgggcgcc gcgactctgt
      301 ctagaatgac ctcgaaaaac gatttttcgg ccgcttatcc atgaagatcg ccacctggaa
      361 cgtgaactcg gtgaaggcgc ggctcgaagc cgccacggcc tgggcgaagg aggccgtgcc
      421 cgacgtggtt tgcctgcagg agatcaaatg caccgacgac accttccccg ccgccgcctt
      481 cgaggatctc ggctataact cagccgtaca cggacaaaag acctataacg gcgtggcgat
      541 cctgtcgaaa cgcccgctgg aggacgtcac cccgcgcctg cccggcggcg acggcgacga
      601 gcatgcccgc tacctcgagg cgctggtgcc cggcggcaag ggcatggtgc gcgtggcgtc
      661 gatctacgcg cccaacggca accccatcgg cacggagaag ttcacctaca agctggcgtg
      721 gctggagcgc ctctacgaac gcgcccgcgc cctgctcgcc ttcgaggaac cggcggtgct
      781 gatgggcgac tataatgtca tcccgacatc cgaggattgc tgggacccag ccgcctgggc
      841 gacagacgcg ctgttccagc cggaatcgcg cgcggcgctg cggcggattg agaatctcgg
      901 ctatacggat gcgttccgca gcggcaaccc gcagccttac cagtacacat tctgggacta
      961 ccaggccggg gcctggcaga aagaccacgg catccgcatc gaccacatcc tgctgtcgcc
     1021 gcaggcgctc gaccgtttga aggcttgcgc catcgacaaa cgcgtgcgcg gccgcgagaa
     1081 gccgtcggac cacgtgccgg tgtggtgcga actggatatc tgacgccatt taacactgag
     1141 cttgacccaa cattgctcac catggccggg cttgacccgg ccatcctgga tgggcggctc
     1201 tatggccgcc catggtgaga cgatttgttg ggctcgactt aatcacaagc caataaaaac
     1261 ctagctcgcc tcgccagtcg cagaaccaca acgcaaacgc ctttcaacgg tcccatctgc
     1321 tcctcatttg cgccgcaatt ttctcgccta actagcctgc aaaattcgta tgcaaggaga
     1381 gttgcgggta tgcgcgcagg cgcggcgttt gtcttttttc tggtcgctct gacgaccggc
     1441 attgccgccg ccgcaccggc caagctcgtg tatcggatcg atcgcgccac cgcgaccatc
     1501 gcccacaacc gttttgtcat cgtcgcaaga ggcgcggtga gcaccggcgg ctgggaaaag
     1561 ccgctgctgc gcctgcagaa aacttccgtt tcggaagcat ccacgctgga agtggaattc
     1621 gtggcgacac caccgcatcc tcgcgcggcc gtggcgcatg cgatcctgcc ggtgagtgcc
     1681 cgcctcgccg cgccgctgcc gcgcaccggg gtcgtccagg tgaagatcgt gtcgcagacc
     1741 aacagcgtga tagtgccggt cacgcgcttg agcggcacga gcgcccaacc tcacgccgtg
     1801 ccggccgcgg catacctcgc gcgccaatcg aactaagaga acattattgg aaagtcaggc
     1861 cgcattcgcg acgacgcgcc cggcgcgctt cgcgcgccaa tcaaaccaag ggaaccgcgt
     1921 cctttgcgat attgaaaagt caggctgcat ccgcgacgac gcggttcctg cccgtcttct
     1981 tggcgcgata gagcgcctga tcggcgcggt gcaaaaggtc ttccgcgccg tcgccctcgc
     2041 cttccgaccc ggcgatgccg atgctgatgg tgatggtgag cttgccgggc gcgcggctga
     2101 tttcgatcgg cgtggtttcg atgctcttgc gcaggcgctc cgccaccgaa taggcgtaag
     2161 cgatatcggt atccggcatc accaccacga attcctcgcc gccgaagcgg caggccagat
     2221 cgatgccgcg gacgttggcc gaaatgcgcc tggagaattc gcgcagcact tcgtcgccga
     2281 tgtcgtggcc gtgggtgtcg ttgaccagtt tgaagaaatc gatgtccatg atgacgaagg
     2341 cgagcggctt gccggatttg cgcgcctgag ccagcaggtt atccaggtgg cggctcatgt
     2401 agcggcgatt gtgcaggccg gtcagctgat cggtgatcgc catttccagg ctgagctgga
     2461 cgttgtggcg caaccggtcg gagtagagtt tcttgcgcaa ctgggtgcgc acgcgcgcca
     2521 ccagttcgtt cttgtcgacg ggccgcgtca ggtaatcgtt gacg
//
LOCUS       DASWPU010000415         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_58450,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000415 DASWPU010000000
VERSION     DASWPU010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58450"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VGC27_08960"
     CDS             <1..232
                     /locus_tag="VGC27_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004732527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282740.1"
                     /translation="RTNAEAEGELLGLVASPDGAGARLLTEAAGAMRLTARGYHRVLR
                     VARTIADLGGADGVGRAHIAEALSFRRLAHLH"
     gene            complement(337..918)
                     /locus_tag="VGC27_08965"
     CDS             complement(337..918)
                     /locus_tag="VGC27_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282741.1"
                     /translation="MRIFIAGLLGAIAMFVWTSFAHVATPLANIGVSPMADEPAVIAA
                     MQSGVGGKSGLYFFPWVSPDDPKMMEKEAALLKTNPTGLLIYHPPGASSDMTPRLIQE
                     FAKELAQSLIAAFLLSMTLIAGFFRRTLFVTLIGAFAVLGSNVSYWIWYGFPFSYTLA
                     VITIELVGAFAAGLAIAWWFGRGTVRLAGALER"
     gene            complement(1081..1854)
                     /gene="pstB"
                     /locus_tag="VGC27_08970"
     CDS             complement(1081..1854)
                     /gene="pstB"
                     /locus_tag="VGC27_08970"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY0282742.1"
                     /translation="MDTTVQSAVKVDVRHLSFYYGTNKALRDVTLPIYDRKVTAFIGP
                     SGCGKSTLLRVLNRIYELYPNQRAEGEVFLDGEDILHSKQDTNLLRARVGMVFQKPTP
                     FPMTIYDNIAFGIKLYERLPRSELDGRVESALQRAALWGEVKDKLRESGLSLSGGQQQ
                     RLCIARTVATRPEVILLDEPCSALDPISTAKIEELMDELVNDYTIIIVTHNMQQASRT
                     SDFTAFMYLGEMIEFDNTEKVFTSPTNKQTEQYITGRFG"
     gene            complement(1858..2703)
                     /gene="pstA"
                     /locus_tag="VGC27_08975"
     CDS             complement(1858..2703)
                     /gene="pstA"
                     /locus_tag="VGC27_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY0282743.1"
                     /translation="MKDMKRYSARKRRNGFFLTLSVFAALFGLAWLFVILSSLFYNGF
                     NGISLKVFTEMTPPPGSDGGLANAILGSALMSSLAVAAGTPIGLLAGTYLAEYGRYAY
                     LSFFVRFVNDILLSAPSIVTGLFIYAVLVRPMHSFSAWAGAASLTVIVIPVVVRTTEN
                     MLLLVPNGLREAAAALGAPRNAIIRAVTWRAARAGIVTGVLLAVARISGETAPLLLTT
                     LSNQFWSTDLSKPMASLPVVIFNFAMSPYADWHRLAWAGALLITAAVLVLSIAARILE
                     SSRRS"
     gene            complement(2700..>3661)
                     /gene="pstC"
                     /locus_tag="VGC27_08980"
     CDS             complement(2700..>3661)
                     /gene="pstC"
                     /locus_tag="VGC27_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY0282744.1"
                     /translation="TEKKTIAVKRGGSRDVRFHGVTLIASVAVLVIFAGVIAALLVGA
                     MPSIKAFGLPFIYTDAWNPVTEKFGAQAPIFGTLITSFLAMLLAVPVGIGIAIFLTEI
                     CPRHLRRPIGIAIELLAGIPSIIYGIWGLFVFAPLFQRGLQPWLIDFFANIPVLKSLF
                     AGPPYGIGIFTAAIILSIMVLPFISAVTRDVFETVPSTLKEAAYAVGCTTWEVMWRVV
                     LPFARPGVVGGAMLALGRALGETMAVTFVIGNAHHIRESLFSPGTSISAVIANEFTEA
                     VGELYTASLFELGLILFCITFIVLASAQLMLRALERRAGGVAA"
BASE COUNT          757 a         1142 c         1165 g          597 t
ORIGIN      
        1 gcgcaccaat gccgaagcgg aaggcgaact gctcggcctc gtggcgtcgc ccgacggcgc
       61 cggcgccaga ttgctgacgg aagcggcggg tgcgatgcgt ttgacggcgc gcggttatca
      121 ccgggtgctg cgggtggcgc gcaccatcgc cgatcttggc ggagcggacg gcgtcggccg
      181 cgcccacatc gccgaagcgc tatcgtttcg cagactggcg catctgcatt gaagccacta
      241 tttccagttc cgcgccacgc ttgccgcagc actgctcgtc gtcttgcccg ccgcgttgac
      301 gaaaaaggcc aagccggaag cggccgcctt cctcgcctat ctttcgagcg cgcccgccaa
      361 tcggacggtg cctctgccga accaccaggc gatggcaagg ccggcggcga atgcgcccac
      421 cagttcgatc gtgatgacgg cgagcgtgta gctgaacgga aatccgtacc agatccagta
      481 ggagacattc gaccccaaga ccgcgaaggc gccgatcagg gtcacgaaca gggtgcgccg
      541 gaagaagccc gcgatcagcg tcatcgacag caggaacgcc gcgatcaagg attgggcgag
      601 ttccttggcg aattcctgaa tcaacctcgg cgtcatgtcg ctgctggcgc ccggcgggtg
      661 atagatcagc agtccggtcg gattcgtttt cagcaacgcc gcttcctttt ccatcatctt
      721 cggatcgtcc ggcgataccc agggaaagaa atagagaccg gatttgccgc cgactccgct
      781 ctgcatcgcg gcgatgacgg caggctcgtc cgccataggg ctcacgccga tgttggcaag
      841 cggcgtcgcc acatgcgcga aggacgtcca tacgaacatc gcgatggcgc ccagcaatcc
      901 agctatgaaa atgcgcatcc aaccccccat cgttcgaccc gtccgattat aggttagttg
      961 cgcaaggcgg acaaacttcg cccaaggtaa acttttgcac gggccgcggc ccgccttcgc
     1021 cgccttggcg gcgcgacgcg cggaccgacg aacccctggc cgaggactat atagccgccc
     1081 tcagccgaag cggccggtga tgtattgttc ggtctgcttg ttggtgggcg aggtgaagac
     1141 cttctccgta ttgtcgaact cgatcatctc gcccagatac atgaaggcgg tgaagtcgga
     1201 ggtgcgcgag gcctgctgca tgttgtgggt gacgatgatg atcgtgtaat cgttcaccag
     1261 ttcgtccatc agctcttcga tcttcgcggt cgagatcgga tcgagcgccg agcacggctc
     1321 gtcgagcagg atcacttccg gccttgtcgc cacggtgcgc gcgatgcaca gccgctgctg
     1381 ctgaccgccg gaaaggctga ggccggattc gcgcagcttg tccttgacct cgccccacaa
     1441 ggcggcgcgc tgcaacgcgg attcgacgcg cccgtcgagt tcggagcgcg gcaggcgctc
     1501 gtagagctta atgccgaagg cgatattgtc gtagatcgtc atcgggaacg gcgtcggctt
     1561 ctggaacacc atgccgacgc gcgcgcgcag aagattggtg tcctgtttgg agtgcaggat
     1621 gtcttctccg tcgagaaaca cctcgccttc ggcgcgctgg ttcggataaa gctcgtagat
     1681 gcggttgagc acgcgcaaca gcgtggactt gccgcaaccg gaagggccga tgaacgccgt
     1741 caccttgcgg tcgtaaatcg gaagcgtgac gtccctcagc gccttgttcg tgccgtaata
     1801 gaaagaaagg tgcctgacgt cgaccttcac cgcgctctgg acggtcgtat ccatgcctca
     1861 gctccgcctt gacgattcga gaattcgggc cgcgatgctc aaaacgagca cggcggcggt
     1921 gatcagcaac gcgcccgccc aggcgagccg gtgccaatcc gcgtaaggac tcatcgcgaa
     1981 attgaaaatg acgaccggca gactcgccat cggcttggac aaatccgtac tccagaactg
     2041 attgctcagc gtggtcagca gcaacggcgc cgtctccccc gaaatgcgcg caaccgccag
     2101 cagcacgccc gtgacgatgc ccgcgcgcgc cgcgcgccag gtcacggcgc ggatgatggc
     2161 gttgcgcggc gccccgagcg ccgcggccgc ttcgcgcagg ccgttgggca ccaagagcag
     2221 catgttctcg gtcgtgcgca ccaccacggg aatcacgatc accgtcagcg acgcggcgcc
     2281 cgcccaggcg gagaaactgt gcatcggccg caccagcacc gcgtaaatga aaagtcccgt
     2341 cacgatggag ggcgcactca acagaatgtc gttgacaaaa cggacgaaga acgagaggta
     2401 cgcgtagcgg ccgtattcgg cgagataggt gccggccaaa aggccgatgg gcgtgcccgc
     2461 ggcgaccgcg agggagctca tcaacgcgct gccgagaatc gcattcgcca aaccgccgtc
     2521 ggaacccgga ggcggcgtca tttccgtgaa aaccttgagc gagatgccgt tgaagccgtt
     2581 gtagaagagc gaactcagga tgacgaagag ccaggcgagt ccgaacagcg cggcgaacac
     2641 cgaaagcgtc agaaaaaacc cgttgcggcg cttgcgcgcg ctgtagcgtt tcatgtcctt
     2701 catgccgcca cgcctcccgc tctgcgctcg agcgcgcgaa gcatcaattg cgccgacgcc
     2761 agcacgatga aagtgatgca gaagagaatg aggcccaatt cgaacagcga cgccgtgtag
     2821 agttcaccca ccgcttcggt gaattcgttg gcgatgacgg cggaaatcga cgtacccgga
     2881 gagaacagcg actcgcggat atgatgggcg ttgccgatga cgaaggtgac cgccatcgtt
     2941 tcgccgagcg cgcggcccag cgccagcatc gccccgccca cgacgcccgg ccgggcgaac
     3001 ggcagcacaa cgcgccacat cacttcccag gtcgtgcagc ccacggcgta ggccgcctcc
     3061 ttcagagttg agggtacggt ctcgaagacg tcgcgcgtca ccgccgagat gaacggcagg
     3121 accatgatcg acaggatgat cgcggccgtg aaaataccga tcccataagg cgggcccgca
     3181 aaaagactct taagcaccgg gatattggcg aaaaaatcga tcagccaggg ctgcagcccc
     3241 cgttggaaca gcggagcaaa gacgaacagg ccccaaatgc cgtagatgat gctggggatg
     3301 ccggccagaa gctcgatcgc gatgccgatc ggccggcgca aatggcgcgg gcagatttcg
     3361 gtcaggaaga tcgcgatccc gatgccgacg ggcaccgcca gcagcatggc aaggaaagag
     3421 gtgatgaggg tgccgaaaat gggagcctgg gcgccgaact tctcggtcac cggattccag
     3481 gcgtcggtgt agatgaaggg cagtccgaag gccttgatcg acggcatcgc gcccacgagc
     3541 agggcggcaa taacccccgc gaaaatgacc agcaccgcga cggaggcgat cagggtcacg
     3601 ccgtgaaacc gcacgtctct cgagccgccg cgtttgacgg ctatggtctt tttttcggtg
     3661 g
//
LOCUS       DASWPU010000416         6137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_71277,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000416 DASWPU010000000
VERSION     DASWPU010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71277"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /gene="rfbF"
                     /locus_tag="VGC27_08985"
     CDS             complement(<1..757)
                     /gene="rfbF"
                     /locus_tag="VGC27_08985"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HEY0282745.1"
                     /translation="MRLKENSPRTPNTMKVVILAGGLGTRLMEETDNRPKPMVEIGGI
                     PILCHIMTIYSAYGFNDFVICLGYKGYYIKEYFANYVLHRSDIVVDLSSHSVGFLKTE
                     GIPPWRITLVDTGDATQTGGRLRRVRHILDGDGAFHMTYGDGVADVDIAALTAFHNAH
                     RLDATVTVVRPPGRFGATVVKDKKVVRFEEKPAGDGNLVNGGFFVLNPRVIDRIDDDM
                     TPWETAPLSGLARDGQLGAYVHEGFWQPMDTLRE"
     gene            974..2002
                     /locus_tag="VGC27_08990"
     CDS             974..2002
                     /locus_tag="VGC27_08990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0282746.1"
                     /translation="MLYRRRASGKEGDLSLQGPRVPTPPVTIGMPVYNDERYLRLALD
                     DLLEQSFTDFELIISDNASTDASPEICREYAAKDGRIRYVRQQTNLGALRNFLFLLDQ
                     ARGEFFMWAASDDQWHKEYVARLVAALRRDKSLAVAFSPFQYMDEEGRKIGAMQRIDY
                     SGSWPVARLIKFNMNLNVGADAFFYGLFRRDIIKDLRIRQWRGINSNILTNMAYPVLS
                     FCLARGGFISVGDQPMWMDRIHIHSVPRHSAARNWSPMKYLFAFILRKANLAWYNLEE
                     IYRGRRNPALILALLPFVVLRCIADVLLLVASIAFRREFVVGVSPMPAPARRRPPAVA
                     EPDSRDDG"
     gene            complement(2048..3106)
                     /gene="rfbG"
                     /locus_tag="VGC27_08995"
     CDS             complement(2048..3106)
                     /gene="rfbG"
                     /locus_tag="VGC27_08995"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEY0282747.1"
                     /translation="MPDRSFWSRQRVLLTGHTGFKGAWLSLWLEKLGATVCGFSLEPS
                     TDPSLFLLLEPFQQATSVIGDIRNCAAVNAVVSDFRPTIVVHMAAQSLVRRSYRLPLE
                     TFETNVMGTANVLNALRDKEGVRAALIVTTDKVYRNLEDGHAFVEDDPLGAHDPYSAS
                     KAAAELAAVSWARSFLAKSNVVVATARAGNVIGGGDWSEDRVVPDVWRAVRRGEAPVL
                     RYPNATRPWQHVLEPLSGYLRYLEEIAAGGVEATALNFGPLSHDVMTVAELAEAMLPA
                     LGAGGGWRQEPNVLHREMTALSLDASRAAATIGWRPRLTCRDAIEWTAQWYGAFDKGL
                     DARTICLQQIARYETLQV"
     gene            complement(3210..4433)
                     /locus_tag="VGC27_09000"
     CDS             complement(3210..4433)
                     /locus_tag="VGC27_09000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0282748.1"
                     /translation="MKCRLCNADIEHVFVDLVASPPSNSFLSADGLSEPETYFPLKVM
                     VCHHCWLVQIAESKPAKEIFSSEYVYFSSYSKSWLAHAKRYADMIVPRLGLDGSSFVV
                     EIASNDGYLLRNFVQRKIPCLGVEPAHGTAQAARSLGVETDIEFFGAEYAGTLVRSGR
                     QADLVVGNNVLAHVPDLNDFLGGTELLLKPDGVVTFEFPCLMTLVKEGLFDTIYHEHF
                     SYFSLFVVERAFRDRGLRLFDVERLLTHGGSLRVYGCRDSARWPTSAAVSKVLAEERA
                     AGVAGHAFYEGFQSRANRAKNDFLSFLLEARRDGRKVAGYGAAAKGNTLLNYCGVKAD
                     LMPYIVDGSPHKRGLFAPGSHIPVVAPEHLRQDKPDYIVILPWNLREEISSQLAFVRE
                     WGARVVIAIPKLEIF"
     gene            complement(4430..4981)
                     /gene="rfbC"
                     /locus_tag="VGC27_09005"
     CDS             complement(4430..4981)
                     /gene="rfbC"
                     /locus_tag="VGC27_09005"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY0282749.1"
                     /translation="MKVHETEIKGLLLVELRFLSDDRGFFTRHYCADTLREAGLTKSI
                     KQINHTLTKKTGALRGLHFQAPPMSETKIVRCIRGSVFDVAVDLRAQSPTFLQWRAQE
                     LSAENGMLYVIPDGFAHGYQALTDEVEMFYLHTENYAREFEGGIRYDDPRLAIGWPRA
                     VTDISERDMNLPLLEPGFAGFHL"
     gene            complement(5240..6040)
                     /locus_tag="VGC27_09010"
     CDS             complement(5240..6040)
                     /locus_tag="VGC27_09010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0282750.1"
                     /translation="MKFSIVTISYNQAEFLEQTIRSVLDQDYDDIEYIVVDPGSTDGS
                     REIIERYRERISTIIFEPDRGAADGLNKGFAHAHGDIYGFLNSDDVLLPGAIRRAAAY
                     FRARPETEILMGHEWIIDSEGRKVRKAYTDRFNARAFAYSAGVIAQQSTFFRAGLFKR
                     TKGFDTHLTVYWDGELFLDLLEATEKQLYADDFFGAFRIHGESITGGQKLEDASRAYE
                     LARFERIMGRRWRRSDHLVRLFYRVRKYILEPRALMEWLRYGSINKGL"
BASE COUNT         1310 a         1842 c         1721 g         1264 t
ORIGIN      
        1 tttcgcgcaa ggtatccatg ggctgccaaa agccttcatg gacgtaagct cccaattggc
       61 cgtcgcgcgc caaccccgaa aggggcgcgg tttcccaagg ggtcatgtcg tcgtcgatgc
      121 ggtcgattac gcgtggattg aggacaaaga aacccccatt gacgagattt ccgtcgcccg
      181 caggcttttc ctcgaagcga acgacctttt tgtccttgac cacggtcgct ccgaagcgcc
      241 cgggcggccg cacgacggtc acggttgcgt cgagccggtg cgcgttatga aaggcggtga
      301 gcgcggcgat gtcgacatcg gcgacaccgt caccgtaagt catgtggaaa gcgccgtcgc
      361 cgtcgagaat gtgacgtacc ctcctcaagc gcccgcctgt ctgggttgca tcgccggtat
      421 cgaccagcgt tatgcgccaa ggagggatgc cttcggtctt caggaagccg accgaatgcg
      481 aactaagatc aaccacgata tccgatcgat gcaacacgta attggcgaaa tattccttga
      541 tataatatcc cttgtaaccg aggcagatga cgaaatcatt gaagccatat gcggaataga
      601 tcgtcatgat gtggcaaagg atcgggattc cgccgatctc gaccattggt ttggggcggt
      661 tatcggtttc ctccattagg cgcgtcccga gcccgcctgc caaaataaca accttcattg
      721 tatttggtgt ccttggagag ttttctttca aacgcaaccg agcgcttgcc ccaattcagt
      781 tgacgattta agcatatagc gaggaagctg cctgttccag tgctcgtcac gcaaggccaa
      841 gaaaacctca tggaacgcct tttccgaaat atgaactctt ctctttgcgc cgatcgacgg
      901 gttgtgttgc tttccagtca ctaccactca tcgtgccgca gcagaaagcg ccggcgctcc
      961 ggacttgatt ttcttgcttt atcgccggcg tgcttccggg aaggagggtg acttgagttt
     1021 gcaaggaccg cgcgttccga ccccaccggt caccatcggc atgcccgtct acaatgacga
     1081 acgctacctt cgactggcgc tcgacgatct gctggagcag agctttacgg acttcgaatt
     1141 gatcatctcc gataacgcgt cgacagacgc ttcgcccgag atttgcaggg aatacgctgc
     1201 gaaagacggc cgaattcgtt atgtccggca gcaaacgaat ctcggggctt tgcggaattt
     1261 cctctttctc ctcgaccaag cccgcggcga attcttcatg tgggcggctt cggacgatca
     1321 atggcacaag gaatacgtcg cccgtcttgt cgccgcgctg cgccgcgaca aaagtctggc
     1381 ggtcgcgttt tcgccgtttc aatatatgga cgaggaaggc cggaagatcg gcgcgatgca
     1441 acgcatcgat tactccggct cctggccggt cgcacgcctg atcaaattca atatgaattt
     1501 gaacgtcggc gcggatgcgt ttttttacgg cctttttcgt cgcgacatta tcaaggatct
     1561 gcggatacgt cagtggcgcg gcatcaattc aaacattctc acgaacatgg catatcccgt
     1621 tctgtcgttt tgccttgcgc gcggcggctt tatcagcgtg ggcgatcaac cgatgtggat
     1681 ggaccggata cacattcata gcgttccgcg ccacagcgcc gcccgcaact ggtcccctat
     1741 gaagtacctg ttcgccttca tcctgagaaa ggccaatctg gcgtggtaca atctcgaaga
     1801 aatttaccgc gggcggcgca atccggcgct gattttggcg ctgcttccct ttgtcgttct
     1861 gcgctgcatt gcggacgttc tcttgttggt ggcgagcatc gcgttcaggc gcgaatttgt
     1921 ggtaggcgtc agtccgatgc ctgcaccagc tcgccggcgc cctccggccg ttgccgagcc
     1981 cgattcaaga gatgacggct gatccgccgg tgggtcggcc gcagccggcc gctgcgcgaa
     2041 accgggatca aacctgcaag gtttcgtagc gggcgatctg ttgcagacag atggtccttg
     2101 cgtccaagcc cttgtcgaag gcgccatacc attgcgcggt ccactctatc gcatcccggc
     2161 acgtcaggcg cggccgccag ccgatcgtgg cggctgcgcg cgatgcatct agggacagcg
     2221 ccgtcatctc ccgatgcaag acattgggtt cttggcgcca gccgcctccc gcgccaagcg
     2281 ccggcaacat cgcttcggcg agctctgcga ccgtcatgac gtcatgggat aacgggccga
     2341 aattcagcgc ggtcgcttcg acgcctccgg ccgcaatttc ttcgagatac cgcaaatacc
     2401 ccgacagggg ttccaggacg tgctgccagg gccgcgtcgc gttcggatag cgcaagacgg
     2461 gggcttcgcc gcgacgaacg gcccgccaga catccggaac gacgcggtcc tcggaccagt
     2521 ccccgccgcc gatgacgttt cccgcgcgcg ccgtcgccac cacgacgttc gattttgcaa
     2581 ggaaggaacg cgcccaggat accgccgcca gctcggccgc cgctttcgac gccgaatagg
     2641 gatcgtgcgc accgagcgga tcgtcctcca cgaaggcgtg gccgtcttcc aggttccgat
     2701 acactttgtc cgtcgtcacg atgagcgcgg cgcgtacgcc ttctttatcg cgcaaagcgt
     2761 tcagtacatt cgccgtgccc atgacgttcg tctcgaaggt ttcgagcggc agacgatagg
     2821 aacggcgcac cagcgactgc gccgccatgt gaacgacgat cgtgggacga aaatccgaca
     2881 cgacggcgtt caccgcggca caattgcgga tatcccctat gaccgaagtg gcctgttgaa
     2941 aaggctcaag caacagaaac aggctggggt cggtcgatgg ctccagcgag aagccgcaga
     3001 ccgtggcgcc caatttttcg agccacaatg aaagccatgc gcccttgaat ccggtgtgcc
     3061 cggtcagcag gacgcgttga cgagaccaga atgaacgatc aggcatggaa ttcggtttgc
     3121 tcatgtccgg cagtccacca ataacgaacg cgaggccgtt tgaaaaggcg aagtccccac
     3181 ggcggcgccc atgccggaat gacctccgct caaaagattt cgagcttggg tattgcgatg
     3241 acgacccgcg cgccccattc gcggacgaag gcgagttgag aggagatttc ctcgcgcaaa
     3301 ttccacggca ggatcacgat gtaatcgggc ttgtcctgcc gcaaatgttc cggcgcgacg
     3361 acgggaatat ggctcccggg agcgaacaga ccccgcttat gcggcgaacc gtcgacgatg
     3421 tagggcatca gatccgcctt cacgccgcaa taattcagca gcgtattgcc tttcgcggcc
     3481 gcgccatagc cggcgacctt gcggccgtcg cgacgggctt ccagcagaaa ggacagaaaa
     3541 tcgttctttg cccggtttgc gcgcgattga aagccctcgt aaaaggcgtg gccggccacg
     3601 cccgcggcgc gttcttccgc cagaaccttg cttacggcag ccgacgtcgg ccagcgtgcg
     3661 gaatcgcggc agccatagac gcgcaacgat cccccgtggg tgaggaggcg ctcgacgtcg
     3721 aagagacgca ggccgcgatc gcggaatgcc cgctccacaa cgaaaaggga aaaataggaa
     3781 aaatgctcgt gataaatggt atcgaacagt ccctctttga ccagcgtcat caaacaggga
     3841 aattcaaagg taacaacgcc atccggcttg agcagaagct ccgtcccgcc caggaaatca
     3901 ttgaggtccg gcacatgcgc caggacgttg ttgccgacaa cgaggtcggc ctgccggccg
     3961 ctccgcacaa gcgttccggc atattcggct ccgaaaaact cgatgtcggt ttcgacgcct
     4021 aagcttcgcg ccgcttgcgc cgtaccgtgc gcaggctcga cgccaaggca cgggatcttg
     4081 cgctgcacga aattgcgcag cagatatccg tcgttggacg caatctccac cacgaaggaa
     4141 gagccgtcca gccccaaacg cgggacgatc atgtccgcgt aacgctttgc gtgggcaagc
     4201 cacgatttcg aataggagct gaagtagaca tattcgctgc tgaaaatttc cttggccggc
     4261 ttgctctctg cgatctgcac cagccagcaa tggtggcata ccatcacctt gagcggaaaa
     4321 tatgtttccg gctccgacag gccgtccgcc gaaagaaagg agttcgaagg cggagacgcc
     4381 accaaatcga caaagacgtg ctcgatatcg gcattgcaca atcggcattt cataaatgga
     4441 atcccgcaaa gccgggctca agcaacggaa gattcatgtc gcgctccgag atatcggtca
     4501 cggcccgcgg ccagccgatc gccagcctcg gatcgtcgta gcgaatgccg ccttcaaatt
     4561 cccgcgcgta attctccgta tgcagataga acatctccac ctcatccgtc agagcctgat
     4621 atccgtgcgc gaagccgtcg ggaatgacat acagcatgcc gttctcggcg gaaagttcct
     4681 gcgcgcgcca ttgcaggaag gtgggagatt gcgcgcgcag gtccaccgcg acgtcaaaaa
     4741 cacttccgcg tatgcaacgg acgatcttgg tctcgctcat cggcggagcc tggaagtgca
     4801 gccctcgcag cgcgcccgtt tttttcgtca atgtatggtt tatctgcttg atgctttttg
     4861 taagaccggc ttcacgcaag gtgtcggcac aataatggcg ggtaaagaag ccccggtcat
     4921 ccgacagaaa acggagctcc acaagaagga gacctttaat ttccgtctca tggaccttca
     4981 taacgtcatc ttttggaaac tttggcgcct gacggacatg ctatcgctcg cacgaacaaa
     5041 tttccgatga tgaaaagatt ccgccgctgg atcatcggcc gcaattattc gcgttcaaaa
     5101 tatagcctgc cgctcgtacg gttgacaccc gtttaaatta atccagaccg cggatttacg
     5161 ccagaggggg gtgatcacgg ggatccgatc cccaaactag tccccgtctc gaatgccgcc
     5221 ggtgccgtgt tccgaagcct tacaggccct tatttatcga gccgtagcgc aaccattcca
     5281 tcagcgcgcg cggctcgagg atatatttgc gtaccctgta gaagagccgg accaggtgat
     5341 cgcttcttcg ccaacgccgc cccatgatgc gttcgaaccg cgccaattca taggcgcggc
     5401 ttgcgtcctc cagcttctgc cctccagtga tggactcgcc gtgaattcga aacgcgccga
     5461 agaaatcgtc cgcatacaac tgcttttccg tcgcctccag aagatcgaga aacaactcgc
     5521 cgtcccagta gaccgtaaga tgcgtgtcaa aacccttcgt cctcttgaac agcccggctc
     5581 gaaagaatgt cgactgttgg gcaatgactc cggcgctata ggcgaacgcg cgcgcgttga
     5641 acctgtccgt gtatgccttc ctcactttgc gcccttcgga atcgataatc cattcgtgcc
     5701 ccatgaggat ttccgtctcc ggccgggcgc ggaagtaagc ggccgcgcga cggatggcgc
     5761 cgggaagcag cacatcgtcc gaattcagaa atccgtaaat gtctccatgc gcgtgcgcga
     5821 accccttgtt caaaccatcg gcggcgccgc gatcgggttc aaagatgatt gtggatattc
     5881 tctcgcggta tcgctcgatg atctcccggc ttccgtccgt cgaacccgga tcgaccacga
     5941 tgtattcgat atcgtcataa tcctgatcga gaaccgaacg gatcgtctgt tccagaaact
     6001 ccgcttgatt gtaggatatc gtgacaatcg agaatttcat tccagacctg tcgttgaata
     6061 atcgcttgca ttggagcaat acgattgaag gagagccggt tgcccctgac acgggccgat
     6121 gcacgatata ttctgac
//
LOCUS       DASWPU010000417         6349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_58453,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000417 DASWPU010000000
VERSION     DASWPU010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58453"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /gene="metG"
                     /locus_tag="VGC27_09015"
     CDS             complement(<1..1672)
                     /gene="metG"
                     /locus_tag="VGC27_09015"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY0282751.1"
                     /translation="MTRFLITSALPYINGIKHLGNLVGSMLPADVYARFRRAKGDETL
                     FICATDEHGTPAELAALEAGQDVSTYCREQHAIQQALGERFGLSWDFFGRSSSPQNQE
                     LTQHFARVLWSRGHLEVRTTKQAYSATDKRFLPDRYVIGTCPHCGYDRARGDQCENCT
                     RVLDPADLIQPRSALSGATDIEIRDSAHLFLKQSQFVPQLRKWIESHASDWPNLVYSI
                     ACKWLEEGLQDRGITRDLEWGVPVPDDIGDGKLKGKVFYAWFDAPIEYIGATKEWSDA
                     HGKPDAEWQRWWRPDAPGDVTYFEFMAKDNVPFHTVGFPVTIFGSGENWKLVDRIKGF
                     NWLTYYGGKFSTSQKRGVFMDTALDLLPADTWRWYLMANAPENADSSFTWEHFAAVVN
                     KDLADVLGNFVNRITKFCAARFDGQVPGEGEYGEEERALIAELDRRIGQYSEHLEEIE
                     FRKALAELRAIWVAGNEYLTRAAPWTHVKTDRTKAAVGVRMGLNLVLLFGRLAWPVIP
                     TAARTIHEAIMPAPEIIPWPSVPMAEFLDQLETGHRIKDTDVLFAKIAE"
     gene            1799..1874
                     /locus_tag="VGC27_09020"
     tRNA            1799..1874
                     /locus_tag="VGC27_09020"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1832..1834,aa:Thr,seq:tgt)
     gene            complement(1891..2679)
                     /locus_tag="VGC27_09025"
     CDS             complement(1891..2679)
                     /locus_tag="VGC27_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282752.1"
                     /translation="MRSLPAMALMLFAGCFQEQAWAGAWTLDRGQVQVISAVTYSKAS
                     RRFDNSGKPSQKTLFDKTLVQNCFEYGLTDAVTLYAAPEYVMAQSGGEGQATIRARSA
                     AVEAGARILLLTRIGMFSVQGSGKSAGAFNMSVSAHEASGSQAELRLLYGGNYKFLGF
                     NGFTDVQIAERWIKRPRPNEVTVDATLGLWLSPKTLAMFKSYNIVSGGGEAPYTFYRM
                     HKLELSVVRRIGARWSFQIGGITSPFGQNIVAERGIVSALWYQF"
     gene            complement(2683..4341)
                     /locus_tag="VGC27_09030"
     CDS             complement(2683..4341)
                     /locus_tag="VGC27_09030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0282753.1"
                     /translation="MPASLPARAGGRPELGAREAGLATDASLELYLARQFLPWRRDGE
                     GIVIAAADTSAANLAWLQDTYGGAQIASIDPRALDREIAQRFRDRLSDDAVFSLWSRM
                     PRLSARRTLSRGQAVTFAAGGLAVAVVSCLRPMTAMQVLVAVMSAGFAISTTFRAVLA
                     LLGSRNKKDSAPWSTEGELPVYTVLVPLYREANILPQLAHALSALDYPRDRLDIKFVV
                     EEDDRETRAAAEALKAQGPFEILPVPASLPRTKPKACNYALRFARGEYLVIYDAEDRP
                     EPDQLRKAVESFRLAPPETVCLQARLTIDNTDDGWLARMFALDYAVWFKALLPGLDKL
                     GVPMPLGGTSNHFRMAALRAAGGWDPFNVTEDADLGIRLAQLGYRVSMLDSTTFEEAP
                     ARLDSWLRQRSRWLKGYTQTWLVHARDPVALVRHVGWRGAGVLQLFLGGAIWSALFNP
                     VLWLVFAVSCVCSTPYGDLNILGTLARISGLGLFTANMMLVGLAVSGERNKLAMVPYA
                     LCFTFYWALISVAAYRGLWQLVFKPFYWEKTPHGQVRTGSVPGV"
     gene            complement(4619..6112)
                     /gene="glpK"
                     /locus_tag="VGC27_09035"
     CDS             complement(4619..6112)
                     /gene="glpK"
                     /locus_tag="VGC27_09035"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY0282754.1"
                     /translation="MEAPFLLAVDQGTTSTRAILFDGGGKPLASHSIELRQIYPANGW
                     VEHDAEEIWHSVAACCRIVLRGVAAKDVAGIGIANQRETTLIWDRKTGRAIHNAIVWQ
                     DRRTAERCHTLRAMELEGKIAAKTGLLLDPYFSATKAKWLLDNVPGAAERAEKGELAF
                     GTVDSWLITKLTGGALHATDATNASRTMLLDLKTLSWDEDLLALFGIPRSLLPEVRDS
                     AGDFGATAPEIFGAPVRILGVAGDQQAAAIGQACFSPGDVKSTYGTGCFALVNTGPAV
                     PSSQNRLLATCAYRLGGKATYAVEGSIFVAGAVVQWLRDALGVIRSAGEVEALARAAK
                     KAGGLYFVPAFIGLGAPYWDPDARGAILGLTRDMGAAEIARAALDAVCYQTRDLMEAM
                     ARDMHSAGLGAPSAVKVDGGMVENDWFCQRLADLTGLPVERPKVVETTALGAAYLAGL
                     EAGLFCDTGDIAARWALDRRFEPSLTAALRDSLYAGWKEAVARVRAG"
BASE COUNT         1201 a         2031 c         2029 g         1088 t
ORIGIN      
        1 cttcggcgat cttggcgaaa agcacatcgg tatccttgat ccgatgtccc gtctcgagtt
       61 gatcgaggaa ctccgccatc ggcacggacg gccagggaat gatctccggc gccggcatga
      121 tcgcttcgtg aatcgtgcgc gccgcggtcg ggatcaccgg ccaggccagg cgaccgaaca
      181 gaagcacgag attgaggccc atgcgcacgc ccaccgccgc cttggtgcga tcggtcttga
      241 catgggtcca gggcgccgcg cgcgtcagat attcgttgcc ggcgacccag atggcgcgca
      301 gttcggccag cgctttgcgg aattcgatct cttccagatg ctcggaatat tggccgatac
      361 ggcggtcgag ttccgcgatc agcgcccgct cctcctcgcc atattcgccc tcgcccggaa
      421 cctgcccgtc gaagcgcgcg gcgcagaact tggtgatgcg gttgacgaaa ttgccgagca
      481 catcggccag gtccttgttc acgaccgccg cgaaatgctc ccaggtgaac gacgaatcag
      541 cgttctccgg cgcattggcc atcagatacc agcgccaggt gtcggccggc agcagatcca
      601 gcgccgtatc catgaagacg ccgcgcttct gcgaggtgga gaacttgccg ccgtaatagg
      661 tcagccagtt gaagcccttg atgcggtcca ccagcttcca gttctcgccc gaaccgaaga
      721 tcgtgaccgg gaagccgacg gtgtggaacg gcacattatc cttggccatg aattcgaaat
      781 aggtcacgtc gccgggcgca tccggccgcc accagcgctg ccattccgca tcgggcttgc
      841 cgtgcgcatc cgaccattcc ttcgtcgcgc cgatatattc gatgggggcg tcgaaccagg
      901 cgtagaacac cttgcctttc agtttgccgt cgccgatgtc gtcgggaacc ggcacgcccc
      961 attccaggtc gcgggtgatg ccgcggtcct gcaagccctc ctccagccat ttgcaggcga
     1021 tggaatagac caggttcggc cagtccgagg catggctctc gatccatttg cgcagttgcg
     1081 ggacgaattg cgattgcttg aggaacagat gcgcgctgtc gcggatttcg atgtcggtgg
     1141 cgcccgagag cgcggagcgc ggctggatga gatcggccgg atcgagcacc cgcgtacagt
     1201 tctcgcactg gtcgccgcgg gcgcggtcgt agccgcaatg cggacaggtg ccgatcacat
     1261 agcggtccgg cagaaagcgc ttatcggtgg cggaataggc ctgcttggtg gtgcgcacct
     1321 ccagatggcc ccggctccac aggacgcgcg cgaagtgctg ggtcagttcc tgattctggg
     1381 ggctggagga gcggccgaag aagtcccacg acaggccgaa gcgctcgccg agcgcttgct
     1441 ggatagcgtg ctgctcccgg caataggtgg agacgtcctg cccggcttcc agcgccgcca
     1501 gctccgcggg cgtgccgtgt tcgtcggtgg cgcagatgaa cagcgtctcg tcgcccttgg
     1561 cgcgccggaa gcgggcatag acgtcggccg gcagcatgga gccgaccaga ttgcccaaat
     1621 gcttgatgcc gttgatgtat ggcagggcgc tggtgattag gaatcgtgtc atgagaaccg
     1681 gtggttgctg gatcgaagct gggccgggag ataacaggcg cgggcgaatc cgtccaacct
     1741 tttcgctcgc gccggtcggg ttgcacgccg ccctgggtcg taatatgccc gtgggcaagc
     1801 cggcttagct cagttggtag agcacgtgat ttgtaatcac ggggtcgcgg gttcgagtcc
     1861 cccagccggc accacaacca aggccctatt ctagaactga taccataggg ccgaaacgat
     1921 gccccgctcg gcgacgatgt tttgtccaaa cggcgaggta atgcctccga tctgaaacga
     1981 ccatcttgcg ccgatccggc ggacgacgga caattcgagc ttgtgcatgc ggtagaacgt
     2041 ataaggcgcc tcgcctcctc cgctgacgat attatagctc ttgaacatcg ccagtgtttt
     2101 gggtgagagc cagaggccca gggtggcgtc gaccgtcacc tcgttcggcc tgggccgctt
     2161 gatccagcgc tccgcaattt gcacgtcggt gaaaccgttg aaccccagaa acttgtagtt
     2221 tccgccgtac agtaggcgaa gctccgcctg gctgccggag gcttcgtggg ccgataccga
     2281 catgttgaaa gcgccggccg acttgcccga cccctggacc gagaacatgc cgatgcgcgt
     2341 gagcagaagg atgcgcgcgc ccgcttcgac ggccgcgctg cgggcgcgga tcgtcgcctg
     2401 accttcgccc ccggactgcg ccatcacata ttcgggcgcc gcgtaaagcg tgaccgcatc
     2461 cgtcagaccg tattcgaagc agttctgaac gagtgttttg tcgaataaag tcttttgcga
     2521 cggcttgccg gaattgtcga accggcgcga ggccttgctg taggtcacgg cggaaatgac
     2581 ctgcacttgg ccgcgatcga gcgtccaggc gcccgcccat gcctgctcct ggaagcatcc
     2641 ggcgaaaagc atcagcgcca tggccggaag agaccgcatg gcctagacgc cgggaacgga
     2701 gccggtgcgg acttgcccgt gcggcgtctt ctcccagtag aacggcttga aaacgagctg
     2761 ccaaaggcca cggtaggccg cgacggaaat cagcgcccaa taaaaggtga agcagagcgc
     2821 gtatggcacc atggccaatt tgtttcgctc gccggaaaca gcgaggccca cgagcatcat
     2881 attggcggtg aacaggccaa gccccgagat ccgcgccagc gttcccaaga tgttcaggtc
     2941 gccatatggg gtcgaacaga cgcaggagac ggcaaagacc agccacagca cgggattgaa
     3001 aagggccgac cagatggcgc cgccaaggaa cagctgcaac acgccggcgc cgcgccagcc
     3061 gacgtgcctg acgagcgcaa ccggatcgcg cgcatgcacc agccaggtct gcgtatagcc
     3121 cttgagccag cgcgaacgct gcctgagcca ggaatccagc cgcgcgggag cttcttcgaa
     3181 cgtcgttgaa tcgagcatcg agacgcggta gcccagttgc gccaggcgaa tgcccagatc
     3241 ggcatcttcg gtgacgttga acggatccca gccgcctgcc gcgcgcagcg ccgccatacg
     3301 gaaatggttc gacgtgccgc cgagcggcat cggaacgcca agcttgtcga gcccgggcaa
     3361 caacgccttg aaccacaccg cgtaatcgag cgcgaacatg cgcgccagcc agccgtcgtc
     3421 cgtattgtcg atggtcagcc gggcttgcag gcagaccgtt tcgggcgggg ccaggcggaa
     3481 actttcgacg gctttgcgaa gctgatcggg ctcggggcgg tcttccgcgt cgtagatcac
     3541 caaatattcg ccgcgcgcga accgcagggc gtagttgcag gccttcggct tggtgcgcgg
     3601 caggctggcg ggaacgggaa ggatttcaaa ggggccctgc gccttcaacg cctccgccgc
     3661 ggcgcgggtt tcgcggtcgt cttcttccac cacgaatttg atgtcgaggc ggtcgcgcgg
     3721 gtagtcgagc gccgaaagcg cgtgcgcaag ttgaggcaag atattcgctt cgcggtagag
     3781 cggcaccaag accgtataga ccggcaactc gccttcagtt gaccatggtg cggaatcctt
     3841 cttgttgcgg cttcccagca aggcaagcac ggcgcgaaac gtcgtgctga tcgcaaatcc
     3901 ggccgacatc acggccacca gaacttgcat agccgtcatc ggccgcaagc aagagacaac
     3961 cgcaacggcc aagccgccgg cggcaaacgt cacggcttgg ccgcgcgaaa gcgtccggcg
     4021 ggcggatagt ctgggcatcc tcgaccacag cgaaaaaacg gcgtcgtccg aaagccggtc
     4081 tcggaaccgc tgcgcgattt ctctatcgag ggcgcgggga tcaatggacg cgatttgcgc
     4141 gccgccatag gtgtcttgga gccaagccag attggcggcg ctcgtatcgg ccgccgcaat
     4201 cacaattcct tccccgtccc tgcgccaggg caggaattgg cgcgcgagat acaattccag
     4261 gctggcgtcc gtggcgaggc cggcttcgcg ggcgcccaac tccggcctgc cgcccgcgcg
     4321 cgcaggcaga gaagcaggca ataaggcgcg cgacttggct cgcgtggaaa ccgcacgcga
     4381 cacacttcgt gggtccaaga gagcctcctg acccgatcat cggcagagat ttcagtgagc
     4441 cgatcccccc agatccggcg acacgacggc tggacccgtc acgccggcag attcggcttt
     4501 acaaccgcga cgctccacga ttccctacac agcgttcgga ttaacaccgc cgtgatattt
     4561 tccggcaata tatctgtggt tgtaatcgga aaagatcaac aggcgaagtg gaacggcttc
     4621 aacccgcgcg tacgcgcgcg acagcctctt tccagcctgc gtaaagggag tctcgcagcg
     4681 ccgccgtaag cgacggctcg aaacggcgat ccaacgccca gcgggcggca atgtcgccgg
     4741 tatcgcaaaa cagccccgcc tccaagcccg cgagataggc ggcgccaagc gcggtggtct
     4801 cgaccacttt cggacgctcg accggcaagc cggtgagatc ggcgagccgc tggcagaacc
     4861 aatcgttctc caccattccg ccgtcgacct tgacggccga cggcgcgccg agacccgccg
     4921 aatgcatgtc gcgcgccatc gcttccatca ggtcgcgcgt ctgatagcaa accgcatcga
     4981 gggcggcgcg cgcgatttcg gccgcgccca tatcgcgcgt caggcccagg atcgcgccgc
     5041 gcgcgtcggg gtcccagtaa ggcgcgccca gcccgataaa agccgggacg aaatagagac
     5101 cgccggcctt cttggccgcc cgcgccagcg cctcgacctc cccggcgctg cggatcacgc
     5161 cgagcgcgtc gcgcagccac tgcaccaccg cgccggcgac gaaaatgctg ccttccaccg
     5221 cataggtcgc cttgcctccc agccgatagg cgcatgtcgc cagaagacgg ttctgcgagg
     5281 acggcaccgc gggtccggtg ttgacgagcg cgaaacatcc cgtgccgtaa gtcgatttga
     5341 cgtcgcccgg cgaaaagcag gcttgtccga tggccgccgc ctgctggtcg cccgcaacgc
     5401 cgagaatccg gacgggggcg ccgaaaattt ccggcgccgt tgcgccgaag tcgccggcgc
     5461 tgtcgcgcac ctcgggcagc agcgagcgtg gaatgccgaa cagagcgaga aggtcctcgt
     5521 cccacgacag cgtcttcaga tcgagcagca tggtgcgcga ggcgttggtc gcgtcggtgg
     5581 cgtgcagcgc gccgccggtc agcttagtga tcagccagga atcgaccgtg ccgaaagcca
     5641 gttcgccttt ttcggcgcgt tcggcggcac cgggcacatt gtcgagcagc catttggcct
     5701 tggtcgcgga gaaataggga tcgagcagca gcccggtctt ggcggcgatc ttgccttcaa
     5761 gctccatcgc gcggagcgta tggcagcgtt ccgcggtccg ccggtcctgc cagacgatgg
     5821 cgttgtggat ggcgcggccc gtcttgcggt cccatatgag tgtcgtttcc cgctggttgg
     5881 cgataccgat gccggcaaca tccttggccg cgacgccccg cagaacgata cggcagcagg
     5941 ccgcgacgga gtgccaaatc tcctcggcgt cgtgctcgac ccagccattg gccggataga
     6001 tctgccgcag ctcgatggaa tgggaggcca acggcttgcc gccgccatcg aacaggatgg
     6061 ctctggtcga ggttgtgccc tgatcgacgg ccaatagaaa aggcgcttcc aagatcatct
     6121 ccgtaggccg ctctcatcct cgaaaattag attaacacgc tgttaacgcc attttgttac
     6181 ttctctttgg gcggccgtaa gggattgccg cactgcaacc ggggaccgac agtgggactg
     6241 accgcgcggg acatgagcga tatgggacgg ctcgcccaac tggccgccaa cccgcaggac
     6301 acgacgcgcg aggagatttt ccttgccgtc gcctcgcttt accgcgtcc
//
LOCUS       DASWPU010000418         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_58458,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000418 DASWPU010000000
VERSION     DASWPU010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58458"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VGC27_09040"
     CDS             complement(<1..498)
                     /locus_tag="VGC27_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEY0282755.1"
                     /translation="MAAKSVKKVVLAYSGGLDTSVILKWLQTEYGAEVVTFTADLGQG
                     EEIEPARKKAELLGIKPQNIFIEDLREEFVRDYVFPMFRANALYEGVYLLGTSIARPL
                     IAKRQIEIAEKTGADAVCHGATGKGNDQVRFELTYYALKPDIKIIAPWREWNLTSRTR
                     LIEFAE"
     gene            591..1028
                     /locus_tag="VGC27_09045"
     CDS             591..1028
                     /locus_tag="VGC27_09045"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0282756.1"
                     /translation="MAMTSLKFATAVHIMLLLAHEGAAAPARAAASSHLAGSIGANAV
                     VVRRVLASLAAAGLIATRAGANGGAWLAKKPAKIRISEVYAALEEPPGPGFRPKGSAA
                     CPVGRAAPGVICGVIGAMRKASEDALSKLTLADLLKKLEAAAH"
     gene            1112..1699
                     /locus_tag="VGC27_09050"
     CDS             1112..1699
                     /locus_tag="VGC27_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEY0282757.1"
                     /translation="MTRVAIVYHSGFGHTHEQAKAVAAGAAGVAGVEAELIRIESATQ
                     DFAPVLAKLEKADAIVFGAPTYMGSVSGPFKVFMDSTSKSWGKWNGKIAAGFTNSASQ
                     SGDKLSSLIQLAIFGAQHGMLWVSLGLMPGNNVTTGSVEDLNRLGSFLGAMAQSPADA
                     GPDVGPSASDKKTAEHLGHRVAETAKQWNTALVDA"
     gene            complement(1917..2210)
                     /locus_tag="VGC27_09055"
     CDS             complement(1917..2210)
                     /locus_tag="VGC27_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282758.1"
                     /translation="MLGTDRDSIRESVMAKKMPLPKAVTRPKPKAKAAKPPKRAAAPR
                     KKTVRRKKAGKPESTFKKIENAILVGVAEADEVALSLGLLGATPPRKSRKKRR"
BASE COUNT          409 a          749 c          732 g          428 t
ORIGIN      
        1 ctcggcgaac tcgatcagcc tggtgcggct ggtcaggttc cattcgcgcc agggcgcgat
       61 gatcttgatg tccggcttga gcgcgtaata ggtgagttcg aaacgcacct gatcgttgcc
      121 cttgccggtg gcgccgtgac acaccgcgtc ggcgccggtc ttctcggcga tctcgatctg
      181 gcgcttggcg atcagcggcc gcgcgatgga ggtaccgagc aggtacacgc cctcgtagag
      241 cgcattggcg cggaacatgg ggaacacata atcgcggacg aattcctcgc gcaaatcctc
      301 gatgaagatg ttctgcggct tgatgccgag cagctcggcc ttcttgcgcg cgggttcgat
      361 ctcctcgccc tgaccgagat cggcggtgaa ggtgacgacc tccgcgccat attcggtttg
      421 cagccatttc aggatcaccg aagtgtccag cccgcccgaa taggccagca ccaccttctt
      481 cacgcttttc gcagccaccg tttcatgtcc ttaaggaaag gcgggaatat acggaccccg
      541 gcccgaaata ccagcggccc taggcaagtg ccgcgacaac tactatctct atggcgatga
      601 ccagtctgaa gttcgccacc gccgttcata tcatgctgct gctggcgcat gagggcgccg
      661 ccgcgcccgc gcgcgcggcg gcctccagcc atctggcggg cagcatcggg gccaacgcgg
      721 tggtggtgcg ccgcgtgctc gcctcgctcg ccgccgccgg gctgatcgcc accagggcgg
      781 gcgcgaacgg cggcgcctgg ctcgccaaaa agcccgcgaa aatccgcatc agcgaggtct
      841 atgccgcgct ggaagagcca cccggccccg gcttccgccc caaaggaagc gcggcctgcc
      901 cggtgggccg cgccgcgccc ggcgtgatct gcggcgtcat cggcgccatg cgcaaggctt
      961 cggaagacgc gctctccaaa ctgaccctgg cggacctgct gaaaaaactc gaagccgcgg
     1021 cgcactgaac cggccggttg ccccttgaaa tgcatttttg ccggcttata tgtaacagta
     1081 ctagttacta ttactttaag aggcggaagc catgacccgc gttgccatcg tttaccattc
     1141 gggcttcggc cacacccacg aacaggccaa ggcggtcgcg gccggcgccg ccggtgtggc
     1201 gggcgtcgag gcggaactga tccgcatcga gagcgcgacg caggatttcg cgcccgtcct
     1261 cgccaagctg gaaaaggccg acgcgatcgt cttcggcgcg ccgacctata tgggatcggt
     1321 atcggggccg ttcaaagtct tcatggactc gacttccaag tcctggggca agtggaacgg
     1381 caaaatcgcc gccggattca ccaattcagc cagccaaagc ggcgacaaac tgtcgagcct
     1441 gatccagctt gcgattttcg gcgcccagca cggcatgctg tgggtcagcc tcggcctgat
     1501 gccgggcaac aacgtcacca ccggctcggt ggaggatttg aaccggcttg gctcgtttct
     1561 cggcgcgatg gcgcaatcgc ccgccgatgc gggaccggac gtcggcccgt cggcctccga
     1621 caagaaaacg gcggagcatc tcggccaccg cgtcgccgag accgccaagc agtggaacac
     1681 ggcgctcgtt gacgcctgag tttcacaccc ttcggcaagc tcacccttcg gcaagctcag
     1741 ggtgaggggt aacaatgagg ggtaacaaaa gaccttcatg gtgagcttgt cgaaccatga
     1801 gggtgaacga actgaatatc tttaagacgg tctgccccct ccggccgtct taaccgcccc
     1861 gctccggcct tcctccccaa ttccgaggtc ggggcggggc ccctgccttt tggcgatcag
     1921 cggcgctttt tgcggctctt gcggggcggc gttgcgccga gaagccccag cgacagcgcg
     1981 acttcgtcag cctccgccac gccaaccagg atggcgttct cgatcttctt gaacgtgctc
     2041 tcgggcttcc ctgccttctt gcggcgaacc gttttcttgc gcggcgcggc ggcgcgcttg
     2101 ggcggcttgg cggctttcgc cttgggcttg ggcctggtaa cggctttagg cagcggcatc
     2161 ttcttggcca tgacactctc tcgaatggaa tcgcggtcgg tgccgagcat agagggcgca
     2221 tcaggcgatg aaaaccggcg ttaacggcgg ggaagcatgg cgcgtggaac gaaagaccgt
     2281 ccaccgcgtt tatgggtcga gtttctcaag gggcgcgc
//
LOCUS       DASWPU010000419         8614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_27746,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000419 DASWPU010000000
VERSION     DASWPU010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27746"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="VGC27_09060"
     CDS             complement(<1..1367)
                     /locus_tag="VGC27_09060"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY0282759.1"
                     /translation="MSNEHRLYPVILSGGSGTRLWPLSRSAMPKQLLALDGAQTMIQN
                     TVLRAGLPGAVPPILICNDAHRFLVAEQMQAIGVRPGAIVLEPQGRNTAPAAAVAALI
                     AAESDPKAIVLLLPSDHVVAKEAAFRDAVAVALRAAEAGNIVTFGMAPTKPETGYGYI
                     QRGGALPGIDGAFKVRRFVEKPDGETAARYLADGGYFWNSGMFVFRADVLLEETARHV
                     PEVMPAVREAVAKAKRDPDFIRLDGESFARAPNISIDYGVMEHTGRAALVPCAIGWSD
                     VGSWSSLWEIQEQDEQGNVLQGDVIAYDTRGSFVRSEKGLVALVGARDLAVVATKDAV
                     LVADKSRAQDVKAIVDRLKESKRSEAAEHARVFRPWGNYETLEMGEGFQVKRIVVNPG
                     GRLSLQMHHHRAEHWIVVQGTARITCNDKTFLLSENESTYIPIGATHRIENPGKVPLH
                     IVEVQS"
     gene            complement(1513..2976)
                     /locus_tag="VGC27_09065"
     CDS             complement(1513..2976)
                     /locus_tag="VGC27_09065"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEY0282760.1"
                     /translation="MWSKTVGGVAALWLFAGMAVASPWAEAGDARLRSDIMVLAAAGV
                     IDDITMQWPLPWGGILDRLEEPGALGEQPDYVRDAAMRVRARGMAETETHKPRASLTL
                     DAASGPSTVRGFDAMGRQTVQGRVLLEYLWDTTAVHLAAGAQTTNRIDHQAMVPDGSY
                     IAQRLGNATVYAGYKTHWWGPGWVSAMSLSNNARPVLQIGIARAEMAPFESPWLSWIG
                     PWQAEFFIGLLDGPRAAKNTVYTGARFAFSPLPHLEIGFSRTTEMCGSGHECKPLAGY
                     FGLLNDDKHVNLTNDEATIDLRYSGAFAGWAYEIYTQAMNEDTSPFVHSGTSHLFGSS
                     VWVPIEDGVGRLTFEYADSLATKNIWGGGIMHGTAYNNYDYVDGMRYRGRTLGFSLDS
                     DSQLVSVQANFTDQSSRSFTLAYHHTNISDPLNAWGNVVTAAPVTINLAEGRFSQPLR
                     LFERSILFDIAARLQDDQPRPDKGFQASVEVALTVSL"
     gene            complement(3121..4152)
                     /locus_tag="VGC27_09070"
     CDS             complement(3121..4152)
                     /locus_tag="VGC27_09070"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0282761.1"
                     /translation="MRFSRMAVLAAAIAAAAFAVARAEPGGGAAAAKARQLMNSGIAH
                     ERLGRHEEALADFTAALKLKALSPPDRVRAVFDRGVALDVLGRTKDAIRDYSEAIRLD
                     GRFAPALSNRANAYRRLDRLAEAKRDYLAALSADNNAREYPYYGLGQIAEKLGDAETA
                     RDYYRKALTANPGYALAAQSLAALNHAQQAPSVQRPPPAEPQPNRPAAKMAVETASYV
                     HLPGPAARQTPPKSPPVRITERDVAALPLRQAIVEGRPKPAADPPGALIQLGAFRDEA
                     SARGGWNKIVGASEGVLQGKSPLLEPVDLPGKGRLWRLRTAAGDKSDARRLCAALAAK
                     ELACMVVRE"
     gene            4273..5154
                     /gene="galU"
                     /locus_tag="VGC27_09075"
     CDS             4273..5154
                     /gene="galU"
                     /locus_tag="VGC27_09075"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY0282762.1"
                     /translation="MPNSRPVRKAVLPVAGLGTRFLPATKAVPKEMLPVVDKPVVQYA
                     VEEAREAGIEQFVFVTGRGKHIISDHFDHAYELEALLRAREKTAELKGLLEMLPPTGS
                     VGFVRQQQPLGLGHAVWCARHFIGDEPFAVLLPDDLMVGKPSGLAQMVTAYRRVGGGC
                     IVAAEERPREETKRYGVVDPGKKNGNATEVKALIEKPNPDKAPSTLCIIGRYILQPQI
                     FAELDKQERGAGGEVQLTDAMARLIGRMPFHAVQTSCARYYCGDKVGFLKANIAVGLQ
                     RPDIAPALRAVLKELDA"
     gene            5312..7819
                     /locus_tag="VGC27_09080"
     CDS             5312..7819
                     /locus_tag="VGC27_09080"
                     /inference="COORDINATES: protein motif:HMM:NF017857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HEY0282763.1"
                     /translation="MSPWRSVRTSLLCSVALVAGGLAGILPAGAEKQSEHIRNTYGEI
                     GMLDMPSGHMADDGELAFSMGDVGSTQRYNLSFQALPWAEASFRYSRPFGYLSDRNFY
                     DRSFGLKIRLLRENAIVPDVSVGIRDLLGTGVYSSEYVAASKHIGNFDLTAGMGWGRL
                     SDTDTLSNPFGYVLSSFKTRDRGVADTGGQFNWKQFFHGPKVGVFGGAVWHTPIDNLS
                     LMGEYSSDKYTGEALWGGVKVRSPFNVGLNYQFRALSLSGGWFYGTTYGFTVSIHGDS
                     TETVSSAMRIGPAVPPALVRDNTEQHSALQLMMQRNDHVSVAKAGGPWVHVPTEAEQT
                     KLVLQQALLSEGRGVRNVDIQQTTLVIDAHGQRNAQAQCARYAEIVSAAGTPITSIAM
                     TDLQSAAGNVVFCPVAGHAKYAQADSQDKAASDRITANAVDQATLESKLRADMKSQSL
                     ELAALSLGTSELWVYYYNGSYRLESEAVGRLVRLLMAAAPPSVEVFHLIAINIGVPVQ
                     EVTIARGAMERATLGGVKSATLGDAIALSAPSLDNPALDRAAEHIYPHFYWSLDPKLT
                     EHLFDPDSPLEFMVYADAIAGVQLTRGLTLFVEGTANIWNNYSFNRAAGSQLPHVRTD
                     LLQYVKQGQNGIAALEVDYRTRLARDVFIDVRAGYLEDMYMGVGGQILWRPENSRLSF
                     GMDIYQVWKRDFNRLFGVQEYNVLTGHASVYYRSPWYGLNLNVHVGRYLAGDHGATFE
                     VTRRFSTGVEVGAFATFTNVPAWKFGEGSFDKGIIVRIPFEWSLPIHSQSSYNLHLNS
                     LTRDGGQRLNTDNSLYRELDRTSYGEIVQHLDDIVEP"
     gene            7776..8471
                     /locus_tag="VGC27_09085"
     CDS             7776..8471
                     /locus_tag="VGC27_09085"
                     /inference="COORDINATES: protein motif:HMM:NF022552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HEY0282764.1"
                     /translation="MARLCNTWTILSNLKARRTASVVGAVLLLGGCADTAIFGGDAAA
                     IYAAVKNSWGASGKKVTLEEAAAVPYASMGVRLGNSPQAMIILAGDSDGQRLWTSGAG
                     VAIMTRAGRIVRTAGFPYNLGGYGPGTNSVDKDGVRSLHWQADFPDLKLYSVPIVCRE
                     RPARNETIVILGKDIHTQRVDESCTSEGGSLDWSFGNTFWIDPSSGLVWRSIQHIHPQ
                     LDSIETEILRPPG"
BASE COUNT         1599 a         2677 c         2710 g         1628 t
ORIGIN      
        1 gactgtactt cgacgatgtg cagaggcacc ttgcccggat tctcgatgcg atgcgtcgcg
       61 ccgatcggaa tgtaggtcga ttcgttttcc gacaacagga atgtcttgtc gttgcaggtg
      121 atgcgcgcgg tgccctgcac gacgatccag tgttcggcgc gatggtgatg catttgcagc
      181 gacagccgcc cgcccggatt gacgacgatg cgcttgacct ggaagccctc gcccatctcc
      241 aaggtctcgt aattgcccca gggccggaac accctggcgt gctcggcggc ctcgctccgt
      301 ttcgactcct tgagacggtc cacgattgcc ttgacatctt gcgcgcgaga cttgtccgcc
      361 accaggacgg cgtccttggt ggccaccacg gcgagatcgc gcgcgccgac cagcgccacc
      421 agcccctttt cgctgcgcac gaaggagccg cgcgtgtcgt acgcgatcac atcgccttgc
      481 aggacgttgc cctgttcgtc ctgctcttgg atttcccaca aggacgacca tgagccgacg
      541 tcgctccagc cgatggcgca aggcaccagg gcggcgcgtc cggtatgctc catgacgccg
      601 taatcgatgg aaatattcgg cgcccgcgcg aaactctcgc cgtcgaggcg gatgaaatcg
      661 gggtcgcgtt tggctttcgc caccgcctcg cgcaccgccg gcatgacctc cggcacatgg
      721 cgagcggttt cctccagcag cacgtcggcg cggaacacga acatgccgct gttccagaaa
      781 tagccgccgt cggcgaggta gcgcgccgcc gtttcgccat ccggcttttc gacgaagcgc
      841 cgcaccttga aggcgccgtc gatgcccggc agagcaccgc cgcgctggat gtagccgtag
      901 ccggtttcgg gtttcgtggg agccatgccg aaggtgacga tgttgccggc ttcggccgcg
      961 cgcaaggcca ccgccaccgc gtcgcggaac gccgcctcct tggctacgac atgatccgag
     1021 ggcagcagca ggacgatggc cttggggtcg gattcggccg cgatcagcgc cgcgaccgcc
     1081 gccgccggcg cggtattgcg cccttggggt tcgagcacga tggcgcccgg cctcacgccg
     1141 attgcctgca tctgttcggc gaccaggaag cggtgggcgt cgttgcagat caggatcggc
     1201 ggaaccgcac ccggcagccc ggcgcgcagc acggtgttct ggatcatggt ttgcgcgcca
     1261 tcaagcgcca gcaattgctt gggcatggcc gagcgcgaca gcggccacag ccgcgtgccg
     1321 gaaccgccgg agagaatgac tggatagagg cgatgttcgt tgctcatggg tccgctcttc
     1381 cgctgatcgc gcgctgcctg gccgggagat taggtcgcca gagaccggat gggaattgcc
     1441 gctagtgtcc tttcgattcc gaaattcgcc cgaggccgat atagagaggg agcgaatttc
     1501 ggaatcggga cactagaggc ttaccgtaag ggcgacctcg accgaagcct ggaagccctt
     1561 gtccggacgc ggctgatcgt cctgcaggcg ggcggcaatg tcgaacagga tggaccgctc
     1621 aaacagccgc agcggctggc tgaagcgccc ttccgccaga ttgatcgtta caggtgccgc
     1681 ggtcacgaca ttgccccacg cgttcagggg atcggagatg ttggtatggt gataggcgag
     1741 cgtgaagctg cgggagcttt ggtcggtgaa gttggcctgt accgacacaa gctgggaatc
     1801 gctgtccagg ctgaaaccga gcgtgcggcc gcgatagcgc atgccgtcca cataatcgta
     1861 attgttgtag gcggtgccgt gcatgatgcc gccgccccaa atgttctttg tcgcaaggct
     1921 atcggcatat tcgaacgtca gccgtccgac gccgtcctcg atcggaaccc agacgctgga
     1981 gccgaacaga tggctggtgc cggaatgcac gaacgggctg gtgtcctcgt tcatggcctg
     2041 ggtgtaaatt tcgtatgccc agccggcgaa cgctccgctg tagcgcagat cgatggtggc
     2101 ttcgtcgttg gttagattga catgcttgtc gtcgttgaga agcccgaaat agcccgccag
     2161 cggcttgcat tcatgtccgc tgccgcacat ctcggtcgtg cgggagaagc ctatctccag
     2221 gtgcggcagc ggactgaagg cgaagcgggc gccggtgtag accgtattct ttgccgcgcg
     2281 cggcccgtcc agcaagccga tgaagaactc cgcctgccag ggtccgatcc aactaagcca
     2341 gggcgactcg aacggcgcca tctcggcgcg cgcgatgcct atctggagca cgggacgcgc
     2401 attgttcgac agcgacatcg ccgaaaccca ccccggtccc caccaatgcg tcttgtagcc
     2461 tgcataaacg gtggcattgc cgaggcgctg ggcgatgtag ctgccgtccg gcaccatcgc
     2521 ctgatggtcg atgcggttgg tcgtttgcgc accggcggcc agatgtaccg cggtcgtatc
     2581 ccagagatat tccagcaaaa ctcggccttg aacggtttgg cgtcccatcg cgtcgaagcc
     2641 gcgcaccgtg cttggtccac tggcggcgtc cagcgtcagc gaggcgcgag gcttgtgcgt
     2701 ctcggtctcg gccatgccgc gtgcgcgcac gcgcatggcg gcgtcgcgga cgtaatccgg
     2761 ctgttcgccc agcgcgccgg gctcctcgag ccggtctaga atgccccccc aggggagcgg
     2821 ccattgcatg gtgatgtcgt cgatgacgcc cgcggccgcc aacaccataa tatcggaacg
     2881 cagccgcgca tcgccagcct cggcccaggg actggctacg gccatgcccg cgaaaagcca
     2941 caaggcggca acgccgccca ccgtcttact ccacactatc tccaccttgc gtgctttcgc
     3001 ggccccaaaa ccgctatatt gaattctacg cagagtagaa agtgccgtta tgttactttt
     3061 ttgtaggttt gtgtaatttt catgccgtgg cagaagcatg gcttgtgcgc gccagcggcg
     3121 tcattccctg accaccatgc aggccaattc cttggcagcc agcgcggcgc agagcctgcg
     3181 ggcgtcggat ttgtctcctg cggcggttcg cagacgccac aggcgccctt ttcccggcag
     3241 gtcaacaggc tccagcagcg gggatttgcc ctgcagcacg ccctccgacg cgccgacgat
     3301 cttattccag ccacctcttg cggaagcctc gtcgcggaac gcgccgagct gaatcaaggc
     3361 ccccggcgga tcggcggcag gcttcgggcg cccttcgaca atagcttggc gcagaggcag
     3421 ggcggcgacg tctcgttcgg tgatccgcac cggcggcgat ttgggcggcg tctgccttgc
     3481 cgccggtccc ggcagatgca cataggacgc cgtctcgacg gccattttcg cagcgggacg
     3541 gtttggctgt ggctcggccg gaggcgggcg ctgcacgctc ggcgcctgct gcgcgtggtt
     3601 cagcgccgcc aggctctggg ccgccagcgc atagccggga ttggcggtga gggcctttct
     3661 gtaatagtcg cgcgccgtct cggcgtcgcc cagcttctcg gcgatctggc ccagtccgta
     3721 ataggggtat tcgcgtgcgt tgttgtcggc ggagagcgcc gccagataat cgcgtttggc
     3781 ctcggccagc cggtccagtc tgcgataggc gttggcgcgg ttgctgagcg cgggagcaaa
     3841 gcgcccgtcc aggcggatgg cttcggaata gtcgcgaatg gcatctttcg tccgcccgag
     3901 cacgtccagg gccacgccgc ggtcgaagac ggcgcgcacc cggtccggcg gcgaaagcgc
     3961 tttcagcttc aatgccgccg tgaaatcggc aagcgcctct tcgtgccggc cgagccgttc
     4021 gtgggcgatg ccggagttca tcaactggcg cgccttcgcc gccgcggccc cgccgcctgg
     4081 ttcggcgcgg gcgacggcaa aggccgcggc tgcaatcgcg gcggccagga cggccatgcg
     4141 gctgaaacgc atcaggcgcc tctcctaccg ggtcaataag cgtcacgcta tcgccatcaa
     4201 agcgagtaga cgaaaactgc accgatgtgc gatgaaaggg acccctgggg cgcaacttac
     4261 acacaggttc ctatgcccaa ctcccgaccc gtccgcaaag ccgtcctgcc cgtcgccggt
     4321 ctcggcacgc gctttcttcc ggccaccaag gccgtgccga aggagatgct tccggtcgtc
     4381 gacaagcctg tcgtccagta tgccgtcgaa gaggccagag aagccggtat cgagcagttc
     4441 gtgttcgtca cgggccgcgg caaacacatt atctccgacc acttcgatca cgcctacgaa
     4501 ctggaagctt tgcttcgcgc ccgcgaaaaa acggccgaac taaagggcct gctggagatg
     4561 ctgccgccga ccggctcggt cggtttcgtg cggcaacagc agccgctggg cctgggacac
     4621 gccgtatggt gcgcgcgcca cttcatcggc gacgagccgt tcgcggttct cttgccggac
     4681 gacctgatgg tgggaaagcc aagcgggctg gcgcagatgg tgacagccta tcgtcgcgtc
     4741 ggcggcggct gcatcgtcgc cgcggaggaa cggccccgcg aggagaccaa gcgctacggc
     4801 gtcgtcgatc cggggaagaa aaacggcaac gcgacggaag tgaaagcgct gatcgagaaa
     4861 cccaatcccg ataaggctcc gtcgacgctg tgcatcatcg ggcgctatat tctgcagccg
     4921 cagattttcg ccgaactcga caagcaagag cgcggcgcgg gaggcgaagt ccaactgacc
     4981 gatgcgatgg cgcggctgat cgggcgcatg ccgttccatg ccgtccaaac aagctgcgcc
     5041 cgatactact gcggcgacaa ggtgggcttc ctgaaggcca atatcgccgt cggtcttcag
     5101 cgcccggata ttgccccggc cttgcgggca gtgttgaaag aattggacgc ttgaagaccg
     5161 gctagtcatc gattgcttgg cattgagttt gcagcaaaag tctggataaa tgccctatgg
     5221 ttcgtgggat cgacgaccat taggccggga gggcgcaatt tccattcgta ggactatgcc
     5281 agggcaatat gcctccggct gcaagaggca gttgagtccg tggcggagcg tgcgcacctc
     5341 tcttctttgc agcgtcgcct tggtcgccgg cggcttagcc gggattctgc cggccggcgc
     5401 cgagaagcag agcgagcata tacgcaacac ctacggtgaa atcggcatgc tcgacatgcc
     5461 cagcggacac atggccgacg atggcgagct ggccttttcc atgggcgacg tcggctcgac
     5521 gcaacgctac aatctttcgt tccaggcttt gccctgggcc gaagcttcat tccgttacag
     5581 ccgccctttc ggatacctca gcgaccggaa cttctacgat cgaagcttcg ggctaaagat
     5641 acgtttgctt cgagaaaacg cgatcgttcc ggacgtttcg gtcggtattc gcgacctgct
     5701 cggtacggga gtctacagtt cggaatacgt ggccgccagc aagcatatcg gcaatttcga
     5761 tttgaccgcc ggaatgggct ggggacggct gtcggacacc gacacgcttt ccaatccttt
     5821 tggctatgtt ctgtcttctt tcaagacgcg cgacagggga gtcgccgaca cgggcggcca
     5881 gttcaattgg aagcagttct ttcacggtcc taaggtcggc gtgtttggcg gcgccgtttg
     5941 gcatacgccg atagacaatc tgagtttaat gggggaatac agctccgata aatatactgg
     6001 cgaggcgctt tggggcggcg tcaaggtacg ctctccattc aatgttggac tgaactatca
     6061 gttccgtgcc ttgtctttgt cgggcggatg gttctacggc acaacctatg gcttcaccgt
     6121 aagcatccac ggcgatagca ccgagacggt tagttcggcg atgcggatcg gaccggcggt
     6181 tcccccggcg ctggtgcgcg acaacaccga acagcacagc gcgctgcagc tgatgatgca
     6241 acgcaacgat cacgtctccg ttgccaaagc cggcggccct tgggtgcatg tcccgaccga
     6301 agccgaacag accaagctgg ttctgcaaca agcgctcctg agcgaaggcc gcggcgtgcg
     6361 taacgtcgat atccagcaaa cgaccctggt gatcgacgcg cacggccagc gcaatgcgca
     6421 ggcgcagtgc gcgcgttatg ccgagattgt ctcggcggcg ggaaccccca tcacgtccat
     6481 cgcgatgacc gatctgcaaa gcgccgccgg caacgtggtg ttctgccccg ttgcgggcca
     6541 cgcgaaatat gcgcaagccg acagccaaga caaagcggcc tcggacagaa taaccgcaaa
     6601 cgcggtcgat caagcgactt tagaaagcaa attgcgcgcc gacatgaaaa gtcaatcgct
     6661 cgaattggct gcactgtcgc tcggcacaag cgaattgtgg gtgtattact ataacggcag
     6721 ctaccggctg gaaagcgagg ccgtcgggcg cctcgtccgc ttgctgatgg ccgccgcccc
     6781 gccgtcggtc gaggtcttcc atctcatagc gataaatatc ggcgtgccgg tgcaggaggt
     6841 cacaatcgcg cgcggcgcca tggaacgcgc cacgctggga ggcgtcaaga gcgccacgct
     6901 cggcgacgca atcgcgctaa gcgcgccgtc gctcgacaat cccgctttgg accgtgccgc
     6961 ggagcatatt tatcctcact tctactggtc gctcgatcca aagctgaccg aacatctctt
     7021 cgacccggat tcccccctgg aattcatggt gtacgcggat gccattgcgg gggtgcagct
     7081 gacgcgcgga ttgacgctct ttgtagaagg caccgcgaac atctggaaca attactcgtt
     7141 taatcgcgcg gccggcagcc aactgccgca tgtgcgcacc gaccttctgc aatacgtcaa
     7201 acaagggcag aacgggattg ccgctctgga agtcgactac cgaaccaggc tggcgcgcga
     7261 tgtgttcatc gacgtaaggg cgggctatct ggaagacatg tacatgggtg tcggcggcca
     7321 aatcctgtgg agaccggaga acagccgcct tagcttcggc atggacatct accaagtgtg
     7381 gaagcgcgat ttcaaccgcc tgttcggagt gcaggaatat aacgttctca ccgggcatgc
     7441 ctcggtctat tatcgctcgc cctggtacgg tttgaactta aacgtccatg tcggacgtta
     7501 tctggccggc gatcacggcg cgaccttcga agtgacgagg cgcttctcca ccggcgtcga
     7561 ggtcggcgcc tttgcgacgt tcaccaacgt tccggcctgg aaattcggcg aaggaagctt
     7621 cgacaaaggt atcatcgttc gcatcccctt cgaatggtcg ttgccgatac acagccagtc
     7681 ctcctacaat cttcacttga actcgctgac gcgcgacggc ggacagaggc tcaacaccga
     7741 caattcgctg tatagggaat tagacaggac aagctatggc gagattgtgc aacacctgga
     7801 cgatattgtc gaaccttaag gcccgtcgca cggcgtcggt cgtcggggcc gttcttctgc
     7861 ttggcgggtg cgccgacacg gccatcttcg gcggcgacgc ggcggccatt tatgccgccg
     7921 tcaagaacag ctggggcgcc agcggtaaaa aggtgacgct cgaggaggcc gccgccgtgc
     7981 cttacgcttc gatgggtgtg cgtttgggca acagccccca ggccatgatc atcctggcgg
     8041 gcgactcgga cgggcagcgc ctctggacct ccggcgccgg agtcgcgatt atgacgagag
     8101 ccggccgcat cgtgcgcacg gccggcttcc cctacaatct aggcggctat ggtccgggaa
     8161 ccaattccgt tgacaaggac ggcgtgcggt ctttgcattg gcaggccgat tttccggatc
     8221 tcaagctcta ttccgtcccg attgtgtgcc gggagcggcc ggccagaaac gaaacgatcg
     8281 tgattctcgg aaaggacatt catacccagc gcgttgacga atcatgcacc agcgagggcg
     8341 gcagcttgga ctggtcgttc ggcaacacct tttggatcga cccgtcatcc ggccttgtct
     8401 ggcgctcgat ccagcatatc catccgcaac tcgattcgat tgagacggaa atcctgcggc
     8461 cgcctggctg attatcgggc cgcatggccg gctgccgaag tgccctaaaa taacactggg
     8521 ccctaaaata acactgggga cggcacaacc aaatgcgccg tccccatatg cgtcgcgagt
     8581 tctcgcgaaa aaccgattac gggccgtatg acgt
//
LOCUS       DASWPU010000420         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_76645,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000420 DASWPU010000000
VERSION     DASWPU010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76645"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /gene="dnaN"
                     /locus_tag="VGC27_09090"
     CDS             <1..881
                     /gene="dnaN"
                     /locus_tag="VGC27_09090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY0282765.1"
                     /translation="PDGAQVQAELLTSEGGRLAVSAGTSRFELACLPKEDFPQMAAGA
                     LPHRFRLAADDLKRLIDKTRFAISTEETRFYLNGIYLHAAKDAKAKTLRAVATDGHRL
                     ARYELELPEGAADMPGVIVPRKTVQELRRLLDDADGAIEVSLSDTKIQFAFNGVDLTS
                     KLIDGTFPDYARVIPAGNDKVLALESKEFGQAVDRVSTISADKTRAVKIAVAKDKVTL
                     TVVNPESGTATEDLAATYSAAPLEIGFNARYLLDITGQIEGKSVRLLLSDAGSPTLIE
                     DTDDPRTLYVLMPMRV"
     gene            878..>2047
                     /gene="recF"
                     /locus_tag="VGC27_09095"
     CDS             878..>2047
                     /gene="recF"
                     /locus_tag="VGC27_09095"
                     /inference="COORDINATES: protein motif:HMM:NF000820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEY0282766.1"
                     /translation="MSLNRGIDPHPVRMSAPTSPLQGEEIASRKLAVTRVQLTNFRSY
                     ANAEIAVSGRPVVLAGPNGAGKTNILDAISLLAPGRGLRGAKLSEHIRRGPQLRIPAN
                     VARSNSVAGAVRREDAQQNPESMPSEALWAVAATVFRGAEAYDIGTGLTLGANGGERR
                     AVRLNGVAAQSSADLGEIVQMTWLTPAMDRLFSEGASGRRRFLDRLVLGFDPGHGRRA
                     QRYETAMRERSRLLKFGPRDPMWLDGLEKEMAEAGVAIAASRIAAVTRLNAMLSERGE
                     ASAFPCAQLALEGETDVLLQEFGADTEAKIRALLLRSRVPDAESGHTAFGPHRSDLAV
                     RHTQKRADARECSTGEQKALILNLVLAQAARLSRAESAPNPILLLDEVAAHLDPDR"
BASE COUNT          344 a          701 c          687 g          315 t
ORIGIN      
        1 tcccggacgg cgcgcaggtg caggccgaat tgttgacgtc cgaaggtgga cggctcgcgg
       61 tctcggccgg gacgtcgcgc ttcgagcttg cctgcctgcc gaaggaggat ttcccgcaga
      121 tggcggcggg cgccctgccc catcgcttcc gcctcgcggc cgacgacctg aagcggctga
      181 tcgacaagac gcgcttcgcc atctccaccg aagagacgcg cttctatctc aacggcatct
      241 atctgcacgc cgcgaaggac gccaaagcga agaccttgcg cgccgtggcg accgacggcc
      301 accggctggc gcgctacgag ctggagctgc ccgagggcgc cgccgacatg cccggcgtga
      361 tcgtgccgcg caagacggtg caggaactgc gccgcctgct cgacgacgcc gacggcgcca
      421 tcgaggtgtc gctgtccgac accaagatcc agttcgcctt caacggcgtg gacctgacct
      481 ccaagctgat cgacggcacc tttcccgatt acgcgcgggt gatcccggcc ggcaacgaca
      541 aggtgctggc gttggaatcc aaggagttcg gccaggcggt cgatcgcgtc tctaccattt
      601 ctgccgacaa gacccgcgcg gtgaagatcg ccgtcgccaa ggacaaggtg acgctgacgg
      661 tggtcaatcc ggagtcgggc acggcaacgg aagacctcgc cgccacttac agcgccgcgc
      721 cgctggaaat cggcttcaac gcccgctatc tgctcgatat caccggccag atcgaaggca
      781 agtcggtgcg cctgctgctc tccgacgccg gctcgccgac gctcattgag gacacggacg
      841 atccgcgcac gctctatgtc ctgatgccga tgcgggtgtg agtttgaacc gcggcatcga
      901 cccccacccg gtgcgcatga gcgcgccgac ctctccctta cagggggagg aaattgcgtc
      961 gcgcaagctt gccgtcacgc gggtgcagct gaccaacttc cgctcctatg cgaatgcgga
     1021 gatcgccgtc agcggacgcc cggtggtgct ggccgggccg aacggcgcgg gcaagaccaa
     1081 catcctcgac gccatttcgc ttctggcgcc cggccgcggc ctgcgcgggg ccaagttgtc
     1141 cgaacacata aggcgcggac ctcagctgcg catcccggcg aacgtggccc gcagcaacag
     1201 cgtcgcgggc gcggttcgcc gtgaggatgc gcagcaaaat ccggagtcta tgcccagcga
     1261 agcgctatgg gcggtggcgg ctacggtttt tcgcggcgcg gaagcctacg acatcggcac
     1321 cggactgacg ctcggcgcca atggcggcga gcggcgcgcg gtgcggctca acggcgtggc
     1381 ggcgcaaagt tcggccgatc tgggcgagat cgtgcagatg acctggctga cccccgccat
     1441 ggaccggctg ttcagcgaag gcgccagcgg acggcggcgc ttcctcgacc ggctggtgct
     1501 cggcttcgat cccggccatg ggcggcgcgc acagcgctac gagaccgcga tgcgcgagcg
     1561 ttcgcggctg ttgaagttcg gtccgcgcga tccgatgtgg ctcgacgggc tggaaaagga
     1621 aatggccgag gcgggcgtgg ccatcgccgc ctcgcggatt gccgccgtta cgcggctgaa
     1681 cgcgatgctc tccgaacgcg gcgaagcgag tgcgtttcct tgcgcccagc ttgcgctcga
     1741 aggcgagacc gacgtcctgt tgcaggagtt cggcgccgac acggaagcca agatacgcgc
     1801 cctgttgctc cgctcgcgcg tccccgacgc cgaaagcggc cataccgcct tcggcccgca
     1861 ccgcagcgat ctggccgtgc gccatacgca gaagcgcgcc gacgccaggg aatgctccac
     1921 cggcgagcag aaggcgctga tcctcaacct ggtcctcgcc caggccgcgc ggctttcgcg
     1981 tgcagaatcg gccccgaacc ctatattatt gctggacgag gttgcggcgc atctcgatcc
     2041 cgaccgg
//
LOCUS       DASWPU010000421         3369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_65236,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000421 DASWPU010000000
VERSION     DASWPU010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65236"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VGC27_09100"
     CDS             complement(<1..255)
                     /locus_tag="VGC27_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282767.1"
                     /translation="MDVFTQSGLFALLQVILIDLVLAGDNAVVIGMSAARVHATDRRK
                     VILLGLAAAVVLRVGLAIFAVQLLEYIPLVIAGGILLLWVC"
     gene            complement(286..1134)
                     /gene="hemF"
                     /locus_tag="VGC27_09105"
     CDS             complement(286..1134)
                     /gene="hemF"
                     /locus_tag="VGC27_09105"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEY0282768.1"
                     /translation="MTERQARAQAWFEELRDRICAAFEAIEDEGAFGARPAGRFERKA
                     WIRPAEDGAEAGGGMMSLMHGRIFEKVGVNVSTVYGRFAPDFAETVSGAGQDPRFWAS
                     GISLVAHPHNPHVPTVHMNTRHIVTAKRWFGGGADLTPTVPDERDTADFHAALKRACD
                     GFDPGYYPRFKQWCDEYFFLPHRGEPRGVGGIFYDGLETGDWEKDFAFNREVGLAFLG
                     IYPQIVRRHMERSWTKDEKAHQLVRRGRYVEFNLLYDRGTQFGLKTGGHAEAILMSLP
                     PEARWP"
     gene            complement(1131..1580)
                     /locus_tag="VGC27_09110"
     CDS             complement(1131..1580)
                     /locus_tag="VGC27_09110"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY0282769.1"
                     /translation="MRLALFEPDIPQNCGALIRLGACLGVGLDIIEPCGFLFSDKKLR
                     RAGMDYVEQAEIVRHASWQAFLQARSGLRLVLITTKGAGSYLDFAFRADDVLLLGRES
                     EGVPEEVHKRADARLRIPLKTGRRSLNVAQAGAIVVAEALRQTKGFP"
     gene            1887..2369
                     /locus_tag="VGC27_09115"
     CDS             1887..2369
                     /locus_tag="VGC27_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282770.1"
                     /translation="MSKYDGLGDHLRKQTFAEIPMTFADIEKVTGTKLPPKAQHHRAW
                     WSNNPSNNVMTKVWLNAGYETAQVDMAARKLLFKRMKKTTPPPGGMAEGAREFKPAEG
                     GKKPRRSPLFGALKGTFTIAPGWDLTQPALDPDELAEWEASLDRKADLYEKGLSGKDK
                     "
     gene            2366..2782
                     /locus_tag="VGC27_09120"
     CDS             2366..2782
                     /locus_tag="VGC27_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0282771.1"
                     /translation="MKTPILLDTCAALWIMENAWMRQEAVNAIDEAFDRSEMVFISPI
                     TGWEVGLQAAKGRFKSSYSPQRWLSVLLARPQIALAELPAHVLLESSLLPGNLHKDPA
                     DRIIAATAREYGFTVMTRDRALLAYAADGYLSAVEC"
     gene            2985..>3369
                     /gene="petA"
                     /locus_tag="VGC27_09125"
     CDS             2985..>3369
                     /gene="petA"
                     /locus_tag="VGC27_09125"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY0282772.1"
                     /translation="MTATKPEPTRRDFLYIATGAAGAIGVAFAAWPFIDQMNPTSAVL
                     ALASIEADLSAIQPGQQVVFKWRGHPLFVRRRTPKEITEARAVAVSDLLDPLARNANL
                     PENSPATDANRQIKSEWLVLVGVCTH"
BASE COUNT          704 a          998 c          992 g          675 t
ORIGIN      
        1 gcagacccac aacagcagga tgccgcccgc gatcaccagc gggatatatt cgagcaactg
       61 taccgcgaag atcgccagcc ccacgcgcag aacgacggcg gccgccaaac ccagtaagat
      121 gaccttgcgc cggtccgtgg cgtggacacg cgcggccgac atgccgatga cgacggcgtt
      181 gtctccggcc agcacgaggt cgatcaggat cacctgaagc agcgcgaata gtccgctttg
      241 cgtaaagacg tccaacgatg cgtttcctcg tgtggtgccg tgcggctagg gccagcgcgc
      301 ttccggcggc agtgacatca ggatggcttc ggcatgaccg ccggttttga gtccaaactg
      361 cgtaccgcga tcgtaaagga ggttaaactc cacataacgg ccgcggcgca ccaattgatg
      421 ggctttttcg tctttggtcc agcttcgttc catatgccgc cttacgattt ggggatagat
      481 acccaaaaat gcgagtccaa cttcacggtt gaaggcaaaa tccttctccc aatcgcccgt
      541 ttcaagtccg tcataaaaga tgccgcccac gccgcgcggt tcccctctgt gcggcagaaa
      601 gaagtactcg tcgcaccatt gtttgaagcg gggataataa ccggggtcaa aaccgtcgca
      661 ggcgcgtttc agcgccgcat ggaaatcggc ggtgtcccgc tcgtccggca ccgtcggtgt
      721 caaatccgcg ccgccgccga accatctctt ggcggtgacg atatggcgcg tgttcatatg
      781 cacggtcggc acgtgtgggt tgtgcggatg agcaaccagc gagatgccgc tggcccaaaa
      841 tcgggggtcc tggccggcgc cggaaacggt ctcggcgaag tcgggcgcga agcggccata
      901 cacggtcgag acgttgacgc ccaccttctc gaagatgcgt ccgtgcatca ggctcatcat
      961 cccgccgccc gcttcggctc cgtcttcggc gggccgaatc caggccttgc gttcgaaacg
     1021 gcccgccgga cgggcgccaa acgcgccttc gtcctcgatc gcttcgaacg cggcacagat
     1081 gcggtcgcgc aattcctcga accaggcttg ggcgcgggct tgccgctcgg tcatggaaat
     1141 ccctttgtct gccgcagcgc ctcggcgacg acgatggcgc ccgcctgcgc cacgttgagc
     1201 gaacgcctgc cggtcttcag gggaatgcgc aaccgcgcat cagcgcgctt atggacctct
     1261 tcgggcacgc cttcgctctc gcgccccagc agcagaacat cgtcggcgcg aaaggcaaaa
     1321 tccaggtagg aaccagcacc cttggtggtg atgaggacca ggcgcaggcc ggaacgggcc
     1381 tgcaaaaatg cctgccagga agcatggcgc acgatctcgg cctgctccac atagtccatg
     1441 ccggcacggc ggagcttctt gtcgctgaac aggaagccgc aaggttcgat aatatcgagg
     1501 ccgacgccca ggcaggcgcc cagccggatc agagcaccgc aattttgtgg aatatcgggt
     1561 tcaaacaggg cgaggcgcat gaacaattag cagctaaatc tcagatcatt ccaaaattgc
     1621 ataatgccgc ttcgatggcc gccttcttcg tgggtggaca agaatatttt cgggagtttt
     1681 ggcagccccg attgtacgca actcggcggg taagaccctg ttgagataga atatcggcta
     1741 tccagcaagt ctttggaaca aagccatttt tatctcgtag gtagagttga atttccctgt
     1801 aagttgccat gaaagtcttc ctccattgac acattgtgtg tatgtgtggc acattgtttc
     1861 ttggactgca agcaatggat atttcgatga gcaagtatga cggacttggc gatcaccttc
     1921 gcaagcagac ttttgccgaa attccgatga cctttgccga tatagagaag gtgacgggca
     1981 caaaattgcc ccccaaagcg caacatcacc gcgcttggtg gagcaacaat ccctccaaca
     2041 acgtgatgac caaagtctgg ctgaatgctg gctatgaaac ggcgcaggtt gacatggctg
     2101 cgcgcaagct cctcttcaaa cgcatgaaga aaacgacccc gcctccaggc ggcatggccg
     2161 agggcgcgcg cgaattcaaa cccgcggaag gtgggaaaaa gccgcgtcgt tcacccttgt
     2221 tcggtgcgtt gaaaggcaca ttcacgatag cgcccggttg ggatctaacg cagcccgcgc
     2281 tcgatccgga cgagttggcg gaatgggaag ccagcctcga tagaaaggcg gacctctacg
     2341 aaaaaggtct ttcaggtaag gacaaatgaa gacgccgatt ttgctcgata cctgtgccgc
     2401 gctctggatt atggaaaatg cgtggatgcg gcaggaagcc gttaatgcca tcgacgaggc
     2461 gttcgaccgc agcgagatgg tgttcatatc tcccattaca ggctgggagg tagggctgca
     2521 agctgcgaag gggcggttca aatccagcta ctcgccacag cgttggctaa gcgttttgct
     2581 ggcgcgcccg caaatcgcgc tggccgaatt gcctgcccat gttctgctcg aatcctcgtt
     2641 gttgccgggc aatctgcata aggaccccgc tgaccgcatc atcgcggcca cggcgcggga
     2701 atatggcttt acggtgatga cccgcgaccg cgccctgctc gcttatgccg ccgacggtta
     2761 tctcagtgcc gtggaatgtt gatcgctacg ctggggaaag aatcatgcgt tttcagttcc
     2821 caacacccgg cagggcctgg cattttatgc gtcattctga cgcaagaccc gtcaatacct
     2881 acgttctagt ttagctcctg cgacttaggc gcgccgcttt tttacagaga cggcggagtg
     2941 gtatttgtgt tctctcttcc actctcaaaa aggtgagttt cgtggtgact gcgaccaaac
     3001 cggaaccgac gcggcgggat tttctctaca tcgcgacggg ggcggcaggc gccatcggcg
     3061 ttgctttcgc agcctggccg ttcatcgatc agatgaatcc gaccagtgcg gtgctggcgc
     3121 tggcgtccat cgaggccgac ctttcggcca tccagccggg ccagcaggtt gtgttcaaat
     3181 ggcgcggtca tccgctgttc gtccgccgcc gtacgcccaa ggaaatcacc gaggcgcgcg
     3241 cggttgcagt gtccgatctg ctcgatcctt tggcgcgcaa cgccaacctg ccggagaata
     3301 gcccggcgac cgacgccaat cgccagatca aatccgaatg gctggtgctg gtgggcgtct
     3361 gcacccatc
//
LOCUS       DASWPU010000422         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_46697,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000422 DASWPU010000000
VERSION     DASWPU010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46697"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..2040)
                     /locus_tag="VGC27_09130"
     CDS             complement(112..2040)
                     /locus_tag="VGC27_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0282773.1"
                     /translation="MRAAGWLLASAALLAPNAAMAQDSTTLETVVVTATRTPLSTQKI
                     GSAITVLDAEFIDREQLTTVDEALERVPGVNMTRSGGIGQNTQVRMRGFTTKHVLTMI
                     DGIKLNNPSESDNQYGLEHIFLDSVERIEILRGPQSGLYGGDAVAGVINIITRRGSGE
                     PRLRVSGLYGTENTMQVLLGSEGEIGKVGYTASASYFQTDGISLASRPPGNVERDGYS
                     NLTLSLRGDWAVTDDINIDGWVRYIKSKNDIDANYLPADNPLGLPAYLFQDSSGWSKN
                     DQVMAALHGTWTMLGGHLTHNAQISYVDMTGTYTSPGVEQDSEGLTIEASYYANYTFT
                     PGTFILAGVERKVETGVFEQPVGAGYATVDETIANTGWFATAGVEPVAGMTLSGAVRY
                     DDNEVFGGDTTYRLTGAYNLPDGFDIPGVKTKLRASYGTGGEAPGLRQLLGSSATYQG
                     NPDLHPESTEMLDFGIDQSLESGFARWSLTYYDGTALDGIFNITDPNTGISSPQNITS
                     PVDMKGVEAELYVFPAEWIDFSLSFTNATCIQRSNGQQLFARPLNQISGAVTLRPMEK
                     LSLTLDMYARSKFFSDYPTTWQMPGYGLFNLAASYQINDSLRLIAKVQNLLDKDYEEK
                     LGDSTYGRTAQVRLTYTF"
     gene            complement(2270..2761)
                     /locus_tag="VGC27_09135"
     CDS             complement(2270..2761)
                     /locus_tag="VGC27_09135"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEY0282774.1"
                     /translation="MSRSVPVHDDLPYRPAVGIMLINRGGLVFVGRRIDQTVEGWQMP
                     QGGIDEGETPAQAGLRELEEEVGTDKAVILREMDEWLHYDLPKHLVGVALHGRFRGQK
                     QKWLALRFTGTDRDIDIKTAHPEFAAWKWLPLDELPGLIVPFKRDIYKKVVAAFRDLA
                     KPA"
BASE COUNT          535 a          874 c          940 g          592 t
ORIGIN      
        1 caatccctca tgctgagctt gtcgaagcat gtagttggcg cgatctcgtt taggtggatc
       61 acggacgaga gaagggatgc gggccggttc ggcaccggcc cgcccgccgg ttcagaacgt
      121 ataagtaagc cgtacttgag cggtgcggcc gtaggtggag tcgcccaact tctcctcata
      181 gtccttgtcg aggagattct ggactttcgc tatcagccgc aacgagtcgt tgatctgata
      241 agaagcagcc agattaaaca ggccgtagcc gggcatctgc cacgtcgtgg gataatcgct
      301 gaagaatttc gagcgcgcat acatgtcgag cgtcaacgac aacttctcca tgggccgtag
      361 cgtgacggcg cccgagattt gattgagcgg ccgggcgaaa agctgctggc cgttggagcg
      421 ctggatgcag gtggcattcg tgaaggacag gctgaagtcg atccattcgg cggggaacac
      481 gtaaagctcg gcttccacgc ccttcatgtc gaccgggctg gtgatgttct gcggcgaaga
      541 gatgcccgtg ttcggatcgg tgatgttgaa gatgccgtcc agggcggtgc cgtcgtaata
      601 ggtgaggctc caccgcgcga agccgctttc caggctctgg tcgatgccga aatccagcat
      661 ctcggtggat tcgggatgca gatcgggatt gccttgatag gtggccgacg atcccaggag
      721 ttggcgcagg cccggcgcct cgccgccggt gccgtaggag gcgcgaagtt ttgtcttcac
      781 gcccggaatg tcgaacccgt cgggcaggtt gtaggcgccg gtgaggcgat aggtcgtgtc
      841 cccgccgaag acttcgttgt cgtcgtaacg gacggcgccc gacagcgtca tgccggccac
      901 cggctccacg ccggcggtcg caaaccagcc ggtgttggcg atggtttcgt ccaccgtcgc
      961 ataccctgcg ccgaccggct gctcgaacac gccggtctcg accttgcgtt cgacgcccgc
     1021 aagaatgaac gtgcccggcg tgaacgtgta attcgcataa tagctggcct cgattgtgag
     1081 gccctcgctg tcctgctcga cgccggggct tgtataggtt ccggtcatat cgacatagga
     1141 aatctgcgcg ttatgcgtga ggtgtccgcc gagcatcgtc caggtgccgt gcagggccgc
     1201 catcacctga tcgttcttgc tccagcccga gctatcctgg aaaagatagg cgggcagacc
     1261 gagcggattg tccgccggca ggtagttggc gtcgatgtcg ttcttggatt tgatgtagcg
     1321 cacccagccg tcgatattga tatcgtcggt aacggcccaa tcgccgcgca ggctcaaagt
     1381 caggttggaa tagccgtcgc gctcgacatt gccgggcggg cggctggcga gcgagatgcc
     1441 gtcggtctgg aaatagctgg cgctcgcggt atagcccacc tttccgattt cgccttcgga
     1501 gccgagcaag acttgcatcg tgttctcggt gccgtacagg cccgagacgc gcagacgcgg
     1561 ttcgccgctg ccccgccgcg tgatgatatt gatcacgccg gcgacggcgt cgcccccgta
     1621 gaggccggat tgcggaccgc gcaggatttc gatgcgttcc acggaatcga gaaagatgtg
     1681 ttcgaggccg tattgattgt cggattcgga cggattgttc agcttgatgc cgtcgatcat
     1741 ggtcagaaca tgcttggtgg tgaaaccgcg catgcgcact tgcgtgttct gcccgatgcc
     1801 gcccgagcgc gtcatgttca cgcccgggac gcgttcaagc gcctcgtcga cggtcgtcaa
     1861 ctgttcgcgg tcgataaact ccgcgtcgag taccgtgatg gccgagccga tcttctgcgt
     1921 cgacagcggc gtacgcgttg ccgtgaccac gaccgtctca agcgtggtgc tgtcctgcgc
     1981 catcgcggcg ttcggcgcca gcagcgcggc cgacgcaaga agccaccccg ctgccctcat
     2041 gccgcttgtc ttttgataaa tcaccttgct cctccttttc cctcgaagcg gagaaggacc
     2101 taaatcgcgg tcacaacggg aggatgacgt ttcggggacg acaaaaagac gcctgcgcct
     2161 aaagtcgcct aatggagacg atcccgcgcg aggcggcggc ggattcaacc ttcagtgtac
     2221 ggttgtatgg cgcgacgcat ttacgtccgc aggattgcgg ctccagccgt cacgccggtt
     2281 tcgccagatc gcggaacgcg gcgaccactt tcttgtagat atcgcgcttg aagggaacga
     2341 tcagccccgg caactcgtcc agcggcagcc atttccaggc cgcgaattcc ggatgcgccg
     2401 ttttgatgtc gatgtcgcgg tccgtgccgg tgaaccgcag cgccagccat ttctgcttct
     2461 ggccgcggaa gcgcccgtgc agggcgacgc ccacaagatg cttgggcaga tcatagtgca
     2521 gccattcgtc catctcgcgc aggatgacgg ctttgtcggt gccgacttcc tcctccaatt
     2581 cgcgcaggcc ggcctgggcg ggggtttcgc cctcgtcgat accgccttgc ggcatctgcc
     2641 agccttccac cgtctggtcg atgcgccggc cgacgaaaac cagcccgccg cgattgatca
     2701 gcatgatgcc gacggcggga cggtaaggca gatcgtcatg aacgggaacg gagcgagaca
     2761 taaaacgctt attgtttggc ctgggatact atggccgata cgggaaccag agcaaagccc
     2821 cggcccggaa gtccttttgc ccacacattc acgcggtcga tggtgaccgg atagagaaag
     2881 ccggagccgg acgcgctgcc cttggtgcgc gccgctgatt ccaagtcgga aagccgacgg
     2941 t
//
LOCUS       DASWPU010000423         3560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_71408,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000423 DASWPU010000000
VERSION     DASWPU010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71408"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VGC27_09140"
     CDS             complement(<1..487)
                     /locus_tag="VGC27_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY0282775.1"
                     /translation="MNVFESPDFDAHETVAFFHDKKTGLNSIIAIHSTALGPACGGTR
                     MYPYPDADAALTDVLRLSRGMSYKNAIADLPLGGGKAVILGDPETDKNEARLTAYARA
                     VESLGGRYITAMDVGVGPKDMPVIARATRYVAGFDQPGKKGGDSGPLTALGVFVGLKA
                     AV"
     gene            complement(594..2663)
                     /locus_tag="VGC27_09145"
     CDS             complement(594..2663)
                     /locus_tag="VGC27_09145"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY0282776.1"
                     /translation="MMRRFLLPLLVLLAIPAPAAAQIDHAPKVVARLIADRLAVAPGG
                     TLTVALEEDIRPGWHTYWSNPGDAGAPTEIEWTLPPGWKAGAIQWPYPQRVPVGPLMD
                     YGYEGKIWLLVDLEVPADAKSGEQANLSAAVQWLVCREVCIPEDAVLSLTLFVDAKAA
                     PPDPISAAQFAAARAKIPAVSPWPIRYALAKELRLYVQAPALAGVAKPVQAQFFPARS
                     GLVKDVAPQTLDFADDGVVLRMTPGQKLGNMLDGVLVLTSADNSVQALQVMAHPGEVP
                     SARFEGAVTLTLVWALLFALVGGLILNIMPCVLPVLAMKALALAGHAGADRRHARLES
                     FSYCAGAVASFVAFGLLLVMLRAGGAAIGWGFQLQEPIVVAVLALLMFAVGLNLSGVF
                     EIGPVSAGEGLARRGGLVGAFFTGVLAVAVAAPCTAPFMATAIGYGLTQSTPIVLAVF
                     LALGLGFALPFLLLGLWPAMHRILPKPGAWMLRLKQILALPMYGAAAWLVWVLSQQVD
                     RTGMVVALAALAAFGVSLWLWGATRAGQDWVRKAGAVVAIAGVAGSLFALTLIGHAPA
                     PATGQSEFAGMKSEPYSAARLEELRRARRAVFVDATAAWCITCLVNEEAALSRPAVRA
                     AFADRHVAFLVADWTNRNPQITALLEAHGRSGVPLYLYYAPGAAEAVVLPQILTEGGV
                     LQTIGAK"
     gene            2765..3388
                     /gene="rsmD"
                     /locus_tag="VGC27_09150"
                     /pseudo
     CDS             2765..3388
                     /gene="rsmD"
                     /locus_tag="VGC27_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
BASE COUNT          646 a         1202 c         1179 g          533 t
ORIGIN      
        1 tcaccgccgc cttcaggccg acgaagacgc cgagcgcggt gagcggcccg gaatcgccgc
       61 ccttcttgcc cggctggtcg aagcccgcga cgtatctggt ggcgcgcgcg atcaccggca
      121 tgtccttcgg cccgacgccg acatccatcg cggtgatgta gcgcccgccc aggctctcca
      181 ccgcgcgcgc ataggccgtg agccgcgcct cgttcttgtc cgtctcggga tcgcccagga
      241 tcaccgcctt gccgccgccg agcggcaggt cggcgatggc gttcttgtag ctcatgccgc
      301 gcgacagccg cagcacgtcg gtcagcgccg catccgcgtc gggataggga tacatgcgcg
      361 tgccgccgca cgccggtccc agcgccgtcg aatggatggc gatgatgctg ttcaggccgg
      421 tcttcttgtc gtgaaaaaac gccacggttt cgtgggcgtc gaaatcggga gattcgaaca
      481 cgttcatggc aattctgtcg tgctgttgcg gcggacggta tagcagacgc gcaatcgcca
      541 tgggtctgcg caaacgacgc agcagcactg tgcgtcttgc gttaaccgcg tccttatttg
      601 gcgccgatgg tttgcagcac tcccccttcc gtcaagatct gcggcagcac caccgcctcg
      661 gcggcgccgg gcgcatagta cagatagagc ggcacgcccg agcgcccgtg cgcctcgagc
      721 agcgcggtga tttgggggtt gcggttggtc cagtcggcca ccaggaaggc gacgtgcctg
      781 tcggcgaagg ccgcgcgcac ggcgggccgc gacagggccg cttcctcgtt caccagacaa
      841 gtgatgcacc aggccgcggt ggcatcgacg aacaccgcgc gacgggcgcg gcgcagctct
      901 tccagacgcg ccgccgaata aggctcggac ttcatgcccg cgaattcgct ttggccggtt
      961 gccggcgccg gcgcatggcc gatcaaggtc agcgcaaaaa gcgatccggc aactccggcg
     1021 atggcgacaa cggcaccggc cttgcgcacc caatcctggc cggcgcgcgt ggccccccac
     1081 agccagagcg aaacgccgaa cgccgcgagc gcggccagcg cgaccaccat cccggtgcga
     1141 tcgacctgct gcgacagcac ccacaccagc catgccgccg cgccatacat gggcagcgcg
     1201 agaatctgct tcaggcgcag catccaggcg cccggcttgg gcaggatgcg atgcattgcc
     1261 ggccatagac cgagcagcag gaacggcagc gcaaagccca aacccagcgc caggaagaca
     1321 gcgagcacga tgggcgtcga ttgcgtcagg ccgtagccga tggcggtggc catgaacggc
     1381 gcggtgcaag gcgcggcaac cgccaccgcc aatacgccgg taaaaaacgc tcccacaagc
     1441 ccgccgcggc gcgccagccc ctcgcccgcc gagaccgggc cgatctcgaa cacgccggac
     1501 agattgaggc cgacggcgaa catcaacagc gccagcaccg cgacgacgat gggctcctgc
     1561 aactggaagc cccagccgat ggccgcgccg cccgcgcgca gcatcaccag caacaggccg
     1621 aaggcgacga aactcgccac cgcgccggcg cagtaggaaa aggattccaa acgcgcatgg
     1681 cggcggtccg ctccggcgtg tccggccaaa gccaaagcct tcatcgccag caccggcagc
     1741 acgcagggca tgatgttgag gatcaagccg ccgacgagag caaacagcag cgcccatacc
     1801 agcgtgagag tcacggcgcc ttcgaagcgc gcggacggca cttcgcccgg atgcgccatc
     1861 acctgcaagg cttggacgga gttgtcggcg gaggtgagca ccagcacgcc gtcgagcatg
     1921 ttgccgagtt tctgccccgg cgtcatgcgc agcaccacgc cgtcgtccgc aaagtcgagc
     1981 gtctgcggcg cgacgtcttt caccaggccg gatctggccg ggaagaactg cgcctgcacc
     2041 ggctttgcca cccctgccag cgcgggtgcc tgtacataaa gccgaagctc ctttgccaag
     2101 gcgtaacgta tcggccaggg cgagacggcg ggaatcttcg cgcgcgctgc cgcgaattgt
     2161 gcggcgctga tcggatcggg gggcgccgcc ttcgcatcga cgaacaaggt gaggctcagc
     2221 accgcgtctt cgggaatgca gacctcgcgg caaacgagcc actgaacggc ggcgctcaga
     2281 ttcgcttgct cgcccgattt ggcgtcggcg ggcacctcca gatcgaccag cagccagatc
     2341 ttgccctcat agccgtaatc catcagcggg ccgaccggaa cgcgctgcgg gtatggccat
     2401 tggatggcgc ccgccttcca gcccggcggc agggtccatt cgatttcggt cggtgcgccc
     2461 gcgtcgccgg gattggacca ataggtgtgc cagccggggc ggatatcctc ttccagcgcc
     2521 accgtcagcg tgccgccggg cgcgaccgcg aggcggtcgg cgatcagccg ggcgacaacc
     2581 ttgggcgcat ggtcgatctg ggccgcagcg ggggccggaa tcgccagcag aacgaggagc
     2641 gggagcagga acctgcgcat cattatgctc atagcctgcc cagcggcggc gcgccagcgg
     2701 gctgtcttga ccctcgcctt aaggagatgc tctgcgctag tttccacagg caaagcaggg
     2761 acatttcatg cgcatcaccg gaggcaagtt cggcggccgc acgctggtcg cgccgcgtga
     2821 cgcgcgggtg cgcccgacgt ccgacaaggt gcgccaagcc gttttcaata tcctccttca
     2881 cagtaatttc ggcacggcgc tcgaaggcgc gcgcacggcg gacctgttcg cgggcaccgg
     2941 tgcgctgggc atcgaggcgc tgagccgcgg cgcctcgttc tgcctgttcg tcgacgactc
     3001 ggcggagagc cgcgccctga tccgcgagaa cgtcgaagcg ctccagctta ccggggcgag
     3061 caagatctgg cggcgcgacg cgaccaagct gggaccgatg ggggccggag cactttcccg
     3121 cgaagtgggc accggttcgc ggcagaaagt gcgaccagac aaaaattccg ccggtggacc
     3181 gttcgacctt gtcttcctcg acccgccata ccgcaagggc ctgatcgcgc ccgcactgac
     3241 cagcttgcgc gacggcttct ggctggcgga aggcgctttg ctggtggcgg agatcgccga
     3301 agacgagaac attccgccta cggacggttt cacccggctc gacgaacggg tctatggcga
     3361 tacaagggtg ctgttcctcg gaacgtgaat tccgcccgac cgccgtttca caaggaaggg
     3421 gacgggagac accactgtat gaaagccgtc aactaatccc aattctagcc gagcaagctt
     3481 ggcttgaaat gggagtcccg ggcgttcatt ctgtggtatg gggcagactc agggtcttcg
     3541 ggttgccggg cattaagggg
//
LOCUS       DASWPU010000424         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_46714,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000424 DASWPU010000000
VERSION     DASWPU010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46714"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGC27_09155"
     CDS             <1..267
                     /locus_tag="VGC27_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282777.1"
                     /translation="KVRDVERPRYMLEIATARSDLREFLSGRAGVDVIEADQHHALFT
                     HTKNAAARAKLIRDLLAAGFEVSSFGESTKALEDAYFAQVGQGA"
     gene            264..1622
                     /locus_tag="VGC27_09160"
     CDS             264..1622
                     /locus_tag="VGC27_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282778.1"
                     /translation="MNPEFQRNLWLELTPRRMLLMALVLALVFFAAALTDGAVSGPGQ
                     AARWLYYLIVVVWGTRNAALSVVGEIRDRTWDLQRLSSLGAGAMSWGKLFGSTIYNWF
                     GGAICLAVILADLFANRGVGVTLIDLVYYLAIGVIAQAAAFLASLIAAGRRQSHSQFE
                     VFVYQAVGLAAAVAVYLVWASADPAGSILAGGTPAGVVVWWSAALDARLFLLLSLAIF
                     TAWTLTGCVRQMRLELKMHNGPLVWLGFLLFIGVYVAGFDAWLSQNGALTHLDAVARR
                     LLLAGTAFAALAYLMVFLEPKDRVHYRWLGGAFAKLKFGSALGGLQGWMMAALAALAV
                     GAALFFWLGRTSTPADQATLAAMLGFFTRDVAIIVLASTLSRRRGGDFTAVAVLFALY
                     VLLPAIMNGLKLEAASVFFFPRPSDPVWLSPLLAWSEAVLAAVLAVGTLALPEEKART
                     AE"
     gene            complement(1820..2464)
                     /locus_tag="VGC27_09165"
     CDS             complement(1820..2464)
                     /locus_tag="VGC27_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY0282779.1"
                     /translation="MSIGSKLAPDQILWTFDRFDSIGGAATQVEGHPQLISTQAGKAI
                     LFNGVDDVLFIDKHPLAGAATFTIEAIFRPDGGEFEHRWLHLSEIDPATGTDSDARFL
                     FEHRVVDGQWYLDAFVTGPGYNKTLVVPEKLFPIGKWYHVAQSYDGKIYRSYVNGVLQ
                     AEAAIDFKPQGPGHSSVGARINRVSFFKGAILNALFTPRVLAPKDFEPLPEGLN"
     gene            complement(2551..>2801)
                     /locus_tag="VGC27_09170"
     CDS             complement(2551..>2801)
                     /locus_tag="VGC27_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282780.1"
                     /translation="VIAEEDLRLRGPGELLGTRQSGQVEFRLADLSVHGELLAAARDD
                     AKLVLARDPDLRSPRGEALRVLLYLFGRDEAVRYLRAG"
BASE COUNT          440 a          892 c          917 g          552 t
ORIGIN      
        1 aaggtgcgcg acgtggaacg tccgcgctac atgctggaga tcgccacggc gcgcagcgac
       61 ctgcgcgaat tcctttcggg ccgcgccggc gtcgatgtga tcgaggccga ccagcaccac
      121 gcgctcttca cccacaccaa aaacgcggcg gcgcgcgcca agctgatccg cgacctgctg
      181 gcggcgggct tcgaggtgtc gagcttcgga gaatccacga aagcgctgga agacgcttat
      241 ttcgcgcagg tggggcaggg ggcatgaacc cggaattcca acgcaatcta tggctggaat
      301 tgacgccgcg ccggatgttg ctgatggcgc tggtgctggc gctggtcttt ttcgcggccg
      361 cgctgaccga cggcgcggta agcggtccgg gccaggcggc gcgctggctc tattatctga
      421 tcgtcgtggt ctggggcacg cgcaacgcgg cgctgagcgt ggtgggcgaa atccgcgacc
      481 gcacctggga cctccaacgc ctgtcctcgc tcggcgccgg cgccatgagc tggggcaagc
      541 tgttcggctc gacgatctac aattggttcg gcggcgcgat ctgtttggcc gtgattttgg
      601 ccgacctctt cgccaatcgc ggcgtgggcg tgacgctgat cgacctggtt tattaccttg
      661 ccatcggcgt gatcgcgcaa gcggccgcct tcctcgccag cctgatcgcc gcgggccggc
      721 gccaatccca ttcgcagttc gaggtcttcg tctatcaggc cgtcggcttg gccgcggcgg
      781 tcgccgtcta tctggtctgg gccagcgccg atcccgcggg ctccattctc gccggcggga
      841 cgccggccgg cgtcgtcgtc tggtggagcg cggcgctcga tgcgcgcctg ttcctgctgc
      901 tgtcgctggc gatcttcacg gcctggacgc tgaccggctg cgtgcgccag atgcggctcg
      961 aactcaagat gcacaacggt ccgctggtct ggctcggctt ccttcttttc ataggtgtct
     1021 atgtggcggg cttcgacgcc tggctgtcgc agaacggcgc gctgacccat ctcgacgcgg
     1081 ttgcccggcg gctcctgttg gccggaacgg cgttcgccgc gctcgcctat ctgatggtgt
     1141 ttctcgaacc gaaggatcgc gtgcactatc gctggctggg aggcgcgttc gccaagctca
     1201 aattcggctc cgcgctgggc gggctgcaag gctggatgat ggccgctctc gccgcgctcg
     1261 ccgtcggcgc cgcgctgttc ttctggctcg ggcgcacctc gacaccggcc gatcaagcca
     1321 cgctcgctgc gatgctcggc ttcttcaccc gcgacgtggc gatcatcgtg ctggcgagca
     1381 cgctgtcgcg caggcggggc ggcgatttca ccgccgtcgc cgttctgttt gcgctttacg
     1441 tgctgcttcc ggccatcatg aacggcctga agctcgaagc ggcaagcgtc ttcttcttcc
     1501 cgcgcccgag cgatcccgtc tggctgtcgc cgctgctggc ctggagcgaa gccgtgcttg
     1561 cagccgtgct ggccgtcggc accctggcgt tgcccgaaga aaaagcgcgg acggcggagt
     1621 agggggttag gcaatttgta tttttctgcc cccgcttcgc gggggcagaa aaccacacga
     1681 caagccgata ctagagcttg atccgattta attgaatcgg atcaagctct agattccttg
     1741 attttcgcgc aatctgacgg caaaccgcgt cggcacgctg ctgctaccga ccactttgcc
     1801 ggattgcgct ctaagaagct cagttcagcc cttcgggcag gggttcgaaa tccttgggag
     1861 cgagcacccg cggggtaaag agcgcattca agatggcgcc cttgaagaag cttacgcgat
     1921 tgatgcgcgc gcctaccgag gaatgacccg ggccttgcgg cttgaagtcg atcgcggctt
     1981 cggcctgcaa gacgccgttc acatagctgc gataaatctt gccgtcatag ctttgcgcca
     2041 cgtgatacca tttgccgatg ggaaagagct tttcgggaac caccagggtc ttgttgtagc
     2101 cggggccggt gacgaaggcg tcgagatacc attggccgtc caccacgcga tgctcgaaca
     2161 ggaaccgcgc gtcggaatcg gtgcccgtgg ccgggtcgat ttcggacaga tgcagccagc
     2221 ggtgctcgaa ttcgccgccg tccggccgga agatcgcttc gatggtgaag gtggcggccc
     2281 cggccagcgg atgcttgtcg atgaacagca cgtcgtccac gccgttgaac aggatcgcct
     2341 taccagcttg ggttgagatg agttgcgggt gtccctcgac ctgcgttgca gcgccgccga
     2401 tggaatcgaa acggtcgaag gtccagagga tctgatccgg tgcaagcttg ctgccgatgc
     2461 tcattcgcag tcctttcctg gtgtggtcga tacggtatgc ccccgatcgc ggcgggcggc
     2521 ggacgttgtg gagacgaatc tcataccatc ttatcccgcc ctgagatacc gtaccgcttc
     2581 gtcgcggccg aagagataga gcagcacccg cagcgcttcg ccgcgcggcg agcgcaagtc
     2641 cggatcgcgc gcgagaacaa gcttggcgtc gtcgcgcgcc gccgccagca attcgccatg
     2701 caccgaaaga tcggcgaggc ggaactccac ctgcccgctc tggcgcgtgc cgagcaattc
     2761 gccgggcccg cgcagccgca aatcctcttc cgcgatgacg a
//
LOCUS       DASWPU010000425         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_71440,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000425 DASWPU010000000
VERSION     DASWPU010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71440"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VGC27_09175"
     CDS             complement(<1..297)
                     /locus_tag="VGC27_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282781.1"
                     /translation="MNDLPEKPETAAPPQPPEAAAVASGSPDGADGFAALSGATEPSE
                     EPVPPQPAAGDAAGDEADGKAKHADSWWELAKALFYALLIALVVRTFLFQPFNIP"
     gene            complement(365..775)
                     /gene="acpS"
                     /locus_tag="VGC27_09180"
     CDS             complement(365..775)
                     /gene="acpS"
                     /locus_tag="VGC27_09180"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY0282782.1"
                     /translation="MILGLGSDLIDIRRVEKSIERFGNRFLDRIFTETERLKSDKRTA
                     RAASYAKRFAAKEACSKALGTGLNRGVYWRDMGVVNLPGGKPTMQLTGGALARLQEIT
                     PKGHTAVIDLTITDDFPLAQAIVIISAVPIQSHE"
     gene            complement(858..1598)
                     /locus_tag="VGC27_09185"
     CDS             complement(858..1598)
                     /locus_tag="VGC27_09185"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014605257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY0282783.1"
                     /translation="MSKLRLGVNIDHVATIRNARGGAFPDPVRAAMLAADSGADGITA
                     HLREDRRHISDDDLNRLQRVLHIPLNLEMAATEEMLAIARKHRPHACCIVPENREERT
                     TEGGIDAAGQFDRFEPIVASLTEFAVRVSMFVEPDRIQLDASKALGAPVVELHTGAYS
                     EAHGAEQNRLLENIREAAAYGASIGLEIHAGHGLTYENVQAVAAIPEIRELNIGHYLI
                     GEAIFVGLEVAIRRMRHLMDEARGGGGA"
     gene            complement(1595..2179)
                     /gene="pyrE"
                     /locus_tag="VGC27_09190"
     CDS             complement(1595..2179)
                     /gene="pyrE"
                     /locus_tag="VGC27_09190"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY0282784.1"
                     /translation="MTPDQVLSEFEKAGALLKGHFILSSGLHSDTFLQKALVFQDARR
                     TAKLCKALAEKVRKDVKAEFTAIVSPAVGGIVPGYELGRQLGLPAMYVERQDGKFRLR
                     RGFALAKGQPVLMVEDIVTTGLSSRECLDAIRAAGGKPVAAACLIDRSGGKAKIGVKL
                     VALAALKVPAWEADRLPPHLRGTPAVKPGSRGLA"
     gene            complement(2340..>2722)
                     /locus_tag="VGC27_09195"
     CDS             complement(2340..>2722)
                     /locus_tag="VGC27_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY0282785.1"
                     /translation="GEGAIIHTIDCAELEKAQTSMDDWLDVAWGSHAAEMGPSVARLA
                     VRVKNAAGSLGAAMTAIGNSGGNIFNMKTTNRNPLYFEFQVDIEVRDVAHLQNILGAL
                     RVTAAVESVDRVRGPEEQGVLPSP"
BASE COUNT          482 a          876 c          867 g          497 t
ORIGIN      
        1 cggaatgttg aagggctgga aaagaaaggt gcggacgacc agcgcgatga gcagcgcata
       61 gaaaagcgcc ttggcgagtt cccaccagga atcggcatgc ttggccttcc cgtccgcttc
      121 atcgccggca gcgtctccgg cagccggttg cggcggaaca ggctcctcgg acggctcggt
      181 cgcgccggac aaggcggcga aaccgtcggc gccgtccggc gaaccggacg caaccgcagc
      241 agcttcggga ggctgcggcg gcgccgctgt ttcgggcttt tctggcaaat cgttcatgtc
      301 gacgggaggg aaccggatga aagagggcgc gtcaaggcga atagcatttt aactacgcaa
      361 tgcctcattc gtgcgactga attggcactg cggaaatgat cacgatggcc tgggccagag
      421 gaaaatcgtc ggtgattgtc agatcgatga cggcggtatg gcctttgggc gtgatctctt
      481 gcagccgggc caaggcgccg ccggtcaatt gcatcgtggg cttgccgccg ggcagattga
      541 ccacccccat gtcgcgccag tacacgccgc gattcagccc cgtgccgagc gccttggaac
      601 aagcctcctt ggcggcgaag cgcttggcat aggaggcggc gcgggccgtg cgcttgtccg
      661 acttgaggcg ttcggtctca gtgaaaatcc gatccaggaa acggttgccg aagcgttcga
      721 tgctcttttc gacgcggcgg atgtcgatca ggtcggaacc gaggccgagg atcatggcga
      781 gccctcaccc ttcgacaagc tcagggtgag gggctgggaa aatccctcat gctgagcctg
      841 tcgaagcatg aagaggatca tgcgccgcct ccgccgcgcg cctcgtccat caggtgacgc
      901 atacggcgga tggcgacttc gaggccgacg aagatcgcct cgccgatgag ataatggccg
      961 atgttgagtt cacggatttc tggtatggcg gcgacggctt gcacattctc ataggtcagg
     1021 ccgtggccgg catggatttc gagaccgatg gaggcgccat aagccgccgc ctcccggata
     1081 ttttcgagca ggcgattctg ttccgcgccg tgcgcttcgc tgtaggcccc ggtgtgcagc
     1141 tccaccaccg gcgcacccag cgccttggag gcatcgagct gtatgcgatc cggctccacg
     1201 aacatcgaca cgcggacggc gaattcggtc agcgatgcga caatcggttc gaagcggtcg
     1261 aactgcccgg ccgcgtcgat gccgccttcg gtggtgcgct cttcgcggtt ttcgggaacg
     1321 atgcagcagg catgcgggcg atgcttgcgg gcaatcgcca gcatctcctc ggtcgccgcc
     1381 atctccagat tgagcgggat gtgaagcacg cgctggagcc ggttgaggtc gtcgtcggag
     1441 atgtggcggc gatcctcgcg cagatgcgcg gtgatgccgt ccgcgccgga gtctgccgcc
     1501 agcatcgccg cgcgcaccgg atcgggaaac gccccgccgc gcgcgttgcg gatggtggcg
     1561 acatgatcga tgttcacgcc aaggcgaagt ttgctcatgc cagtccccta cttcctggtt
     1621 tcacggcggg cgtgccgcgc aaatgtggag gaagcctgtc ggcctcccag gccggaacct
     1681 tcagcgccgc cagcgccacc agcttgacgc cgatcttggc cttgccgccg gagcggtcga
     1741 tcaggcaggc cgccgccacc ggcttgccgc ccgccgcacg gatcgcgtcc aggcattcgc
     1801 gcgaagacaa gcccgtggtc acgatgtctt ccaccatcag caccggctgg cccttggcga
     1861 gcgcgaagcc gcggcgcagc cggaacttgc cgtcctggcg ctcgacatac atcgccggca
     1921 ggcccaattg ccggcccagc tcgtagcccg gaacaatacc gccgaccgcc ggcgagacga
     1981 tggcggtgaa ctccgctttc acgtccttgc gcaccttttc ggccagcgcc ttgcacagct
     2041 tcgccgtgcg gcgcgcgtcc tggaacacca gcgccttttg caggaaagtg tcggaatgca
     2101 atccgctcga cagaatgaaa tggcctttca gcagcgcgcc ggctttttcg aattcggaaa
     2161 ggacttggtc cggtgtcatt ctgtcctctt catcctcccc atgagggagg gtcggaaaat
     2221 acaagcggcg cgtagcaaca gcgtacgcac aagaattttc cgaggaaggg ccatgcttca
     2281 caacacccct ccccgaaacg cgtccgtacg ctgtcgctac ggaccgtttt taccctccct
     2341 caaggggagg gtagaacgcc ttgttcctcc ggcccacgca cgcgatcgac cgattcgacc
     2401 gcggccgtca cccgtagcgc gcccaggatg ttctgcaaat gcgccacgtc gcgcacttcg
     2461 atatccacct ggaactcaaa gtaaagcgga ttgcggttcg tcgttttcat gttgaagatg
     2521 ttgccgccgc tgttgccgat ggcggtcatc gccgcgccca gcgaccccgc cgcgttcttc
     2581 acccgcaccg ccagacgcgc caccgacggc cccatttcgg cggcatggct gccccaggcg
     2641 acgtcgagcc aatcgtccat gctggtctgc gccttctcca attcggcgca gtcgatggtg
     2701 tggatgatgg cgccctcgcc cg
//
LOCUS       DASWPU010000426         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_40953,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000426 DASWPU010000000
VERSION     DASWPU010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40953"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(399..1469)
                     /locus_tag="VGC27_09200"
     CDS             complement(399..1469)
                     /locus_tag="VGC27_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0282786.1"
                     /translation="MKGTPKAGLKAHGICQAAATIYDVADHAGVSKTTVSRVLNGEAN
                     VRPATRAVVLKAVKELQFLPNRAARSLASQVEARVGLLYNNPSTAYFSEFLIGALDGS
                     GRNGAQLVVDKCKIGNVEAASEAVHKLVKGGVNGMVLTAPISESAELIEELTEAGISI
                     VAVATGRFSGNVTCVGIDDFKAAYEMTNYLIGLGHRRIGFVKGHPSHTSGVQRARGFE
                     AALRDAGGKVEKPMIAQGYYSYRSGLDAGEQLLSGRKLPTAIFASNDDMASGVMAVAH
                     RKNLSVPRDLSIVGFDDTIAGAVWPGLTTIRQPISDIAAMAIDVIVRNIRDLRAGEKE
                     TPQNHLVPHKLIERESAAAPRT"
     gene            complement(1685..1759)
                     /locus_tag="VGC27_09205"
     tRNA            complement(1685..1759)
                     /locus_tag="VGC27_09205"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1725..1727),aa:Val,seq:gac)
BASE COUNT          327 a          532 c          574 g          380 t
ORIGIN      
        1 gtgggcccgc ccctctttga ctgagcgcaa ccaagcgatc cgcctgtttg gtgtcggtga
       61 gcgaactgcc gtttcctgcg ttacgtctcc cccgcttatg ggagcggtac catttttatt
      121 tcgcgcacta tggttcggtc aagcagatac aagatgctgc actgcaaacg catcgaagat
      181 gtttacggag ggtgactaat aagccacggt gtttaggtct caggcctgac gaatcatggg
      241 ctacggttgt tcgagcaatt gcgccacgat atcgaattgc ctttcacaga aagaaaatta
      301 cggcatcgtc ggcgcgtttc gcctttgcga ttcgtggccg gagtcggcgg cagggctgtc
      361 atgcccggcg ctgggaacgt gccggcgggg ccggggcttt acgtccgcgg cgcggccgcg
      421 gactcgcgct cgatcagctt gtgaggaacg aggtgatttt gcggcgtctc tttttcgccg
      481 gctctgagat cgcggatatt gcgcacgatg acgtcgatcg ccatcgcggc gatgtccgat
      541 atcggctggc gaatggtcgt caaacccggc cataccgcgc cggcgatcgt atcgtcgaag
      601 ccgacgatgg aaagatcgcg cggcacgctc agattcttgc gatgcgcgac cgccatgacg
      661 cccgacgcca tgtcgtcgtt gctggcgaag atcgcggttg gaagcttgcg gcccgacaat
      721 aattgttcgc cggcatccag gccggaacga tagctgtaat agccttgggc gatcatgggc
      781 ttttcgacct tgccgccggc gtcgcgcaaa gccgcttcga agccccgcgc gcgttgcacg
      841 ccggaggtat ggctgggatg tcctttgacg aatccgatgc gtctgtgtcc gagcccgatg
      901 agataattgg tcatctcata ggcggctttg aaatcgtcga tccccacgca ggtgacgttg
      961 ccgctgaagc ggccggtggc gacggcaacg atggagatgc cggcctccgt cagttcttcg
     1021 atcaactcag ccgattcgct gatcggcgcg gtcagcacca tgccgttgac cccgcccttg
     1081 accagtttgt gcacggcctc gctcgcggcc tccacattgc cgattttaca tttgtcgacc
     1141 accagctgcg cgccgttgcg gccgctgccg tcgagcgcgc cgatcaggaa ctcgctgaaa
     1201 taagccgtag aaggattatt atagagcagc ccgacgcggg cctcgacttg gctggccaga
     1261 ctgcgggcgg cccggttggg cagaaattgc agttctttta ccgccttgag aacaacggcg
     1321 cgcgtcgccg ggcggacatt ggcctcgccg ttgagaacgc gggaaaccgt cgttttcgag
     1381 acgcccgcat gatcggcaac gtcgtagatc gtcgcggcgg cttggcatat gccgtgggcc
     1441 tttaggcctg ctttcggcgt gcctttcatt ccggtttcct agcgcgatca agatcatctt
     1501 ctgtcgccgc acgcggtatc gcaagggaca tgagacctgc cgaaacatcg cgcgtcagaa
     1561 cggcgcgcgg catgcgagac ggggaaaggg gcccggttgg cgcccatgag cgccaagctg
     1621 gcaaaccaac gcggcagcca ggttgtcgta cgggcaagtt acatattgtg tccatacagt
     1681 tatatggtgg gcgcgacagg gatcgaacct gtgaccccta ccatgtcaag gtagtgctct
     1741 cccgctgagc tacgcgccct ccgaaggggg gtgagctata gcgcccggca cgcgcggctg
     1801 gcaaggcgta gtt
//
LOCUS       DASWPU010000427         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_120234,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000427 DASWPU010000000
VERSION     DASWPU010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120234"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..666)
                     /locus_tag="VGC27_09210"
     CDS             complement(40..666)
                     /locus_tag="VGC27_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEY0282787.1"
                     /translation="MDRTNTPSPTVRIAVASSDGATVDQHFGQATGFWVFDVAADGIT
                     ARERRDIASHVIGDEGPRDTVCRMLADCKVLLVSRVGVSPQETLAKAGIEASDLHAGK
                     PVATALGEVFAAKSIRHGDAPPDSSGFRLRHAMLRVGDMDRSVDFYTRLLGMRVIERR
                     DHRKNQFTQTYLGYGDDGDQMALELVFNWAREEPYILGDAFGHIAVQV"
     gene            1058..>2182
                     /locus_tag="VGC27_09215"
     CDS             1058..>2182
                     /locus_tag="VGC27_09215"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase subunit alpha"
                     /protein_id="HEY0282788.1"
                     /translation="MPMVLLECDKDIPEREKHIYLKVDGEDTRDFLPVSNAQTIPGTL
                     SERGCAFCGAKLVIGGVLKDTIQMIHGPIGCAYDTWHTKRYPTDNGHFPLKYVWSTDM
                     KESNIVFGGDKRLEKSINEAFAEMPHIKRMIVYTTCPTALIGDDIKAVVKKVMGERPD
                     VDIFTVECPGFAGVSQSKGHHVLNIGWINDKVGTLEPKITSEHTINFIGDYNIQGDTQ
                     LLQKYWERLGIQVISHFTGNANYDDLRCMHRAKLNVVNCARSAGYIANELKKRYGIPR
                     LDIDSWGFNYMAEGIRKICAFFGIEERGEALIADEYGKWKGKLDWYKERLQGTKMAIW
                     TGGPRLWHWTKAVEDDLGVQVVAMASKFGHQEDFEKVIARG"
BASE COUNT          464 a          681 c          634 g          403 t
ORIGIN      
        1 gccggcggcc gccagacggt cgcataatgc ggtgatgttt tacacctgga cggcgatgtg
       61 accgaacgca tcgcccagga tataaggttc ctcgcgcgcc caattgaaca ccagctccag
      121 cgccatctgg tccccatcgt cgccgtaacc gagataggtc tgggtaaatt gatttttccg
      181 gtgatcgcgc cgttcgatca cccgcatgcc caaaagccgc gtgtaaaagt cgaccgagcg
      241 gtccatatcg ccgacccgca acatggcatg ccgcaagcga aagccgctgc tatcgggcgg
      301 cgcatcgcca tgccgtatgc tcttggcggc gaaaacctcg ccaagagcgg tcgccacggg
      361 ctttccggca tgcagatcgc tggcctcgat cccggccttg gcgagcgttt cctgcgggct
      421 gacgccaacc ctggaaacca acagcacctt acaatccgcc agcatgcggc aaaccgtatc
      481 gcgcggcccc tcatcgccga tcacatggct tgcgatatcg cggcgctccc gcgcggttat
      541 gccgtccgca gcaacatcga aaacccaaaa accggtcgcc tggccgaaat gctggtcgac
      601 cgtcgcgcca tcactgctgg cgacggcgat tctaacggtg gggcttggtg tattggtcct
      661 atccatcttg ggtacgtccc ccggcggcag atacattccg cctcggcgca actctcgtgc
      721 caatcgcata ttctcggatt gcgcaggtac cgccgcactt aacgcaccgg ctggaacgtt
      781 tccggataaa ttgtaccgat tggaacggcg cgtcgccgtt cttttgccgg cgcatgccgt
      841 ctgcccgcgc ggcggccggc cgcatatctg cacgggccgg aacgctttct tcgcggttcg
      901 taccaaccgg aacgcggcgg cgcacaaggc cgtgcgaccg gccgtcgcca agcaaggaaa
      961 tcgacgggga cggccggccc cgggtttgtg gcacgcttcc tgctctcctg ctctttgcga
     1021 gcgaagacgc ggccacctta tcctggagat aaccggcatg cctatggtcc ttttggaatg
     1081 cgacaaagac attcccgaac gcgaaaaaca catttatctg aaagtggacg gcgaggatac
     1141 gcgcgatttt cttccggtgt ccaacgcgca aaccattccc ggcacgcttt cggaacgcgg
     1201 ctgcgccttt tgcggcgcca agctggtgat cggcggcgtg ctcaaagaca ccatccagat
     1261 gattcacggg ccgatcggct gcgcctacga cacctggcac accaaacgct atccgacgga
     1321 taacggccat tttccgctga agtatgtgtg gtcgaccgac atgaaggaaa gcaacatcgt
     1381 gttcggcggc gataagcggc ttgagaagtc gatcaacgag gcgttcgccg agatgccgca
     1441 catcaagcgg atgatcgtct acaccacatg tcccaccgcc ctgatcggcg acgatatcaa
     1501 agcggtggtc aaaaaagtca tgggggagcg cccggacgtc gatatcttca cggtcgaatg
     1561 ccccggcttc gccggcgtat cgcagtccaa gggccatcat gtgctcaaca tcggctggat
     1621 caacgacaag gtcggaacgc tggagccgaa aatcaccagc gagcatacga tcaacttcat
     1681 cggcgactat aacatccagg gcgataccca gcttttgcag aaatattggg agcggctcgg
     1741 catccaggtc atctcccact tcaccggcaa cgccaattac gacgatttgc gctgcatgca
     1801 ccgcgccaag ctcaacgtcg tcaattgcgc ccgctcggcc ggctacatcg ccaatgaatt
     1861 gaagaagcga tacggcattc cgcggctcga tatcgattcc tggggcttca actatatggc
     1921 cgaaggcatc cgcaagatct gcgccttctt cggcatcgag gagcgcggcg aggcgctgat
     1981 cgccgacgaa tacggcaaat ggaagggcaa gctggactgg tacaaagagc gtctgcaagg
     2041 caccaaaatg gcgatctgga ccggcgggcc gcggctgtgg cactggacga aagcggtcga
     2101 ggacgatctg ggcgtccagg tggtggcgat ggcgtccaaa ttcggccatc aggaagattt
     2161 cgaaaaggtc atcgcccgcg gc
//
LOCUS       DASWPU010000428         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_83443,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000428 DASWPU010000000
VERSION     DASWPU010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83443"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VGC27_09220"
     CDS             complement(<1..576)
                     /locus_tag="VGC27_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY0282789.1"
                     /translation="MIPNRTALVLGATGGIGGEVSRRLIARGWSVRALHRHLDKLPPD
                     AAPGLVWLKGDAMRAADVAAAAEGVSVIVHAVNPPGYRNWGKLVLPMLDNSIAAARAC
                     GAQIVLPGTVYNFGPDAFPDPHETSPQHPRTVKGRIRAEMERRLRQAALEGGGVLIVR
                     AGDFFGPRAANNWFSQGLVKPGKPVTAITYPG"
     gene            complement(770..1174)
                     /gene="crcB"
                     /locus_tag="VGC27_09225"
     CDS             complement(770..1174)
                     /gene="crcB"
                     /locus_tag="VGC27_09225"
                     /inference="COORDINATES: protein motif:HMM:NF010802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY0282790.1"
                     /translation="MPFQLYAVVALGGALGTAARYFVSGAVANAFSETFPWGTLIINV
                     VGSFVIGFFWTLTVPDWRLFVSGSTRQFVITGFCGGFTTFSSFSLQTMNLMRDGEWLY
                     AGGNILGSVTLCMLGVWLGSLTAALFNQLKGV"
     regulatory      complement(1240..1298)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1454..>2421
                     /locus_tag="VGC27_09230"
     CDS             1454..>2421
                     /locus_tag="VGC27_09230"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0282791.1"
                     /translation="METRQQQIERDDDGIRLDRWFRRHYPALTHGRLEKLLRTGQVRL
                     DGKRAKASDRLEAGQTLRLPPQVLHGALEDKPRTSEPKQSESLEQHVLYMDSSVIVLN
                     KPPGLATQGGSGLSRHVDGMLDSITFEKKQRPRLVHRLDRDTSGVLVVARTVSAAAAL
                     AKSLAQRDASKIYWALTRGVPPKKRGTIKAALAKEGGHGPHGRDERMAVVEDDADDAK
                     LAVTDYVVLGQAGEEFAWVAAKPVTGRTHQIRVHQASLGTPIVGDFKYGGNESRGKGA
                     VADKLHLHARTIDIAHPDGGRLRVVAPLPGHMKKTWELFGFNADDER"
BASE COUNT          478 a          779 c          787 g          377 t
ORIGIN      
        1 gcccggatag gtgatcgccg tgaccggctt gccgggcttg accagccctt gggagaacca
       61 gttgttggcg gccctgggcc cgaagaaatc gccggcacgc acgatcagaa cgccaccgcc
      121 ttccaatgcc gcttggcgca gacggcgttc catctcggcc cggatgcgcc ccttcacggt
      181 gcgcggatgc tgcggcgagg tttcgtgcgg gtcgggaaag gcgtccggcc cgaaattata
      241 gactgtgccc ggcagcacga tctgcgcgcc gcaggcgcgg gccgccgcga tgctgttgtc
      301 cagcatcggc agcaccagtt tgccccagtt gcgatagccg ggcggattga cggcatggac
      361 gatgaccgag acgccttctg ccgccgccgc gacatcggcg gcgcgcatgg catcgccctt
      421 gagccagacc agccccggcg ccgcatccgg cggcagcttg tccagatggc ggtgcagggc
      481 gcgcacgctc caaccccgcg cgatcagccg ccgggacact tctccgccga tcccgccggt
      541 agcgcccaga acgagtgcgg tgcgatttgg tatcaacggt ctctcctttt ggccggtgcg
      601 gcgccaaact ggcattcccg tgctacggaa ttaggtaatt ccgtaccgtc tctatactaa
      661 aatatatgaa tagcgccgaa ccggattggg cctttaccgc gtgaaggctc gctcccgggc
      721 gcatctcgcg cgccttcagc agcacggcgt ccttgggaag ttgcatcgat caaactccct
      781 taagttgatt gaagagcgcg gctgtcagcg atcccagcca gacgccgagc atgcacagcg
      841 tcacggagcc taggatgttg ccgccggcat aaagccattc gccgtcgcgc atcaggttca
      901 tggtttgcag gctgaacgac gagaaggtgg tgaagccgcc gcagaacccc gttatcacga
      961 actggcgcgt cgagccgctg acgaacagcc gccagtcggg gacggtcaag gtccagaaga
     1021 agccgatgac gaacgaaccc accacattga tgatgagcgt gccccaggga aatgtctcgc
     1081 tgaaggcgtt cgctaccgcg ccggagacga aatagcgagc agcggtgccc agcgctccac
     1141 ctagggcaac gaccgcatag agttgaaatg gcactgcagg actcccacaa accgagccgc
     1201 ctgggcgctt ccacctggtt gcgcgccttg cagcatgttc atacggtagg agtcatcagc
     1261 cggaagcggt ttcggggaac cccatcccca tccacctcaa gctaaggaca tcggcgcagg
     1321 gcgtcaatct ggcggaacgg ccgcaaggga actagaagac gacagtctta atgctgcctc
     1381 acccttcgac aagctcaggg tgaggggtat ccttcatgct gagcctgtcg aagcatgacg
     1441 acgcggtcct ttcatggaaa ccagacagca acaaatagaa cgcgacgacg acggcatccg
     1501 gctggaccgc tggtttcgcc gccattaccc ggcgctgacg cacggaaggc tggagaagct
     1561 cctgcgtacc ggacaggtgc ggctcgacgg caagcgcgcc aaggcgagcg accggctgga
     1621 ggccgggcag acgctgcgct tgccgccgca ggtgctccac ggcgcgctgg aggacaagcc
     1681 ccgcacctcc gaaccgaagc aaagcgagag cctggagcag catgtgcttt acatggactc
     1741 ctcggtgatc gtgctgaaca agccgccggg actggcgaca caaggcggtt ccggcctctc
     1801 ccggcatgtc gacgggatgc tggattccat caccttcgag aagaaacagc gcccgcgcct
     1861 ggtgcaccgg ctggaccggg atacgtcagg cgtactggtg gtggcgcgca cggtttcggc
     1921 cgccgccgcg ctggcgaaat cgctggcgca acgcgacgcc tcgaaaatct attgggcgct
     1981 gacccgcggc gtgccgccga agaagcgcgg tacgatcaag gcggcgctgg cgaaggaagg
     2041 cggtcacggc ccgcacggcc gcgacgaacg catggcggtg gtcgaagatg atgcggacga
     2101 cgccaagctg gccgttaccg attacgtcgt gctggggcag gcgggcgagg aattcgcctg
     2161 ggtcgcggcc aagcccgtca ccggccgcac ccatcagatt cgcgtccatc aggcgagtct
     2221 gggaacgcca atcgtcggcg acttcaaata tggcggcaac gaatcgcgcg gcaagggcgc
     2281 cgtcgccgac aagctgcatc tgcacgcccg caccatcgac atcgcccacc ccgacggcgg
     2341 acggctgcgc gtcgtggcgc cgctgccggg gcacatgaag aagacatggg aactgttcgg
     2401 ctttaatgcc gacgacgagc g
//
LOCUS       DASWPU010000429         5812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_40960,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000429 DASWPU010000000
VERSION     DASWPU010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40960"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="VGC27_09235"
     CDS             <1..1521
                     /locus_tag="VGC27_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEY0282792.1"
                     /translation="ARLYELIWKRAIASQMESAELERTTVDVVSADKQVVLRATGTVT
                     LFDGFLTLYQEGHDDEVDENGSKLPRVAAGDAAEIEKVLPAQHFTEPPPRYSEASLVR
                     KLEELGIGRPSTYATILSVLRDRAYVRMDRNRFIPEDKGRLVTAFLSSFFRHYVEYGF
                     TADLEEKLDEVSAGELDWKQLLRDFWTQFSAAVAETKDLKISQVITEIDGILGPHIFP
                     HGADGADPRICPTCGNGRLSLKLGRFGAFVGCSNYPECRYTRQLGQKPGEGGNGEPQP
                     LGIFPATGEQITLRNGRFGPYVQLGEGEKPKRASLPKGTDAASVELELAIKLLSLPRE
                     VGKHPESGEAITANFGRFGPYIAHDGKYASLETPEDVFTIGLNRAVTVLAEKKARGFV
                     RRGQEPLKELGANPNGGALIKLMKGRFGPYVTDGSINATVPRGSDPMALTLEEALALI
                     AERAAKGPAKKKGGHFKKAVKAKPAAEAKAKPEKKPKAPAKKKSAAKHKPAPEAAE"
     gene            1523..3721
                     /gene="rnr"
                     /locus_tag="VGC27_09240"
     CDS             1523..3721
                     /gene="rnr"
                     /locus_tag="VGC27_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY0282793.1"
                     /translation="MPRGKRTNAPPIDKARVLETLAARPGATKRDLARLLNIKGSDRI
                     ALKRILKELEADGLLSGNRKRGYSKPGTLPEVAVLEITGQDMDGELLGRPQTWESNEE
                     PPQIVVVLGEGDAALGRGERILARLSKTSDGYEARIVKRLGASAHRVLGVVRVSGPNV
                     RIAPIDRKSKSEFSVDARERAGAENNELVLAEPIAGRAGGFPRARIVERLGSMDAPKA
                     VSLIAIHAHGIPTDFPKEALDQAAAATPADPRGRTDLRAIPLVTIDPEDARDHDDAVW
                     AGPDPDHKGGHICLVAIADVAHYVTPGSPLDREAYKRGNSVYFPDRVVPMLPETLSAD
                     LCSLKEGVDRPCLAVRMVFDAGGKKRAHQFVRGVMRSAARLTYAQAQSAFDGKPDAAL
                     SPIAKETLAALWKAYGALVKAREIRDPLDLDLPERRIEIGADGKISSIAFRERLESMR
                     LIEEFMVLANVAAAETLEKARTPLIYRVHEEPSKEKLFTFSDYLRTIGVNFAKGQVMK
                     PGVFNRILASVKDGPHEKVMNDVVLRTQAQAIYHPDNLGHFGLNLAKYAHYTSPIRRY
                     ADLIVHRALIRALKLGDGGLTERETARLGEIAEHISMTERRAMAAERDSTDRYVAAFM
                     QDRIGARFDARVTGVTRFGLFVRLAETGAEGLLPARVLGTEYFRHDERRHALVGERTG
                     TVYGLGAIVAVRLAEAAPLTGGLRFDLAEAESAPKTRRPAVVRQRSKRRR"
     gene            4044..4235
                     /locus_tag="VGC27_09245"
     CDS             4044..4235
                     /locus_tag="VGC27_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282794.1"
                     /translation="MGWLIAIGIGQLIVLGYIAYRIEFLLRGDQAAAYTNLVLERLNN
                     IDAWYAAENARIKRDQEWA"
     gene            4338..4505
                     /gene="rpmG"
                     /locus_tag="VGC27_09250"
     CDS             4338..4505
                     /gene="rpmG"
                     /locus_tag="VGC27_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011454349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY0282795.1"
                     /translation="MAKPTTAKIRLNSEGGTGFFYVTKKNTRTMTEKFSMKKYDPVLR
                     KHVQFKEGKIK"
     gene            complement(4653..5570)
                     /locus_tag="VGC27_09255"
     CDS             complement(4653..5570)
                     /locus_tag="VGC27_09255"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0282796.1"
                     /translation="MAGIHANGIDIEYEISGPKDGAPILLIHGFGQQLIAWPDEFVDG
                     LAQAGLRVIRFDNRDTGLTQKWDGQIPDLGAVMAAARERRKPNVPYFLKDMAADAAGL
                     LDALGIASAHILGASMGGMIAQLVALDHPRKTRSLIQIFTTTNDAGLPPSSAEAHQSL
                     ITPPSANDRATVIAHVLKSRRAYASTAFAADDEQAAELIGRAYDRCFYPEGGLRQWAA
                     ILASPPRGKRLKGLKLPALVLHGTADTLLPCAHGRHIADCIQGAEYHEIEGWGHDLPP
                     SVIPLLLGFILPFVERVEAASTTIRHGRA"
BASE COUNT         1131 a         1858 c         1806 g         1017 t
ORIGIN      
        1 gcgcggctct acgagctgat ctggaaacgc gcaatcgcca gccagatgga aagcgcggag
       61 ctggaacgca cgaccgtcga tgtcgtctcc gcggacaagc aggtcgtcct gcgcgccacc
      121 ggcacggtga ctttgttcga cggcttcctt accctctacc aggaaggcca cgacgacgag
      181 gtggacgaaa acggttcgaa gctgccgcgc gtggcggccg gcgacgccgc cgagatcgaa
      241 aaggtccttc cggcgcagca tttcaccgaa ccgccgccgc gctattccga agccagcctg
      301 gtgcgcaagc tggaggaact cggcatcggc cggccttcca cttatgccac catcctgtcg
      361 gtgctgcgcg accgcgccta tgtgcgcatg gatcgcaacc gcttcattcc ggaagacaag
      421 ggccggctcg tcaccgcgtt cctctccagt ttcttccgtc attacgtcga atacggcttc
      481 accgccgacc tcgaagaaaa actcgacgag gtttccgccg gcgaactcga ctggaaacag
      541 ttgctgcgcg atttctggac ccagttctcc gccgccgtcg ccgaaaccaa ggatctgaaa
      601 atctcgcagg tcatcaccga gatcgacggc attctcggcc cgcacatctt cccgcacggc
      661 gccgacggcg ccgatccgcg catctgcccg acctgcggca atggcaggtt gagcctgaaa
      721 ctcggccgtt tcggcgcctt cgtcggctgc tcgaactatc ccgaatgccg ctacacgcgc
      781 caactcggcc agaagccggg cgaaggcggc aacggcgaac cgcagccgct cggcatcttc
      841 cccgcaaccg gggagcagat caccttgcgg aacggccgtt tcggtcccta tgtgcagctt
      901 ggcgagggcg agaaacccaa gcgcgccagt ctgcccaaag gcaccgacgc tgccagcgtc
      961 gaattggagc tggcgatcaa gcttttgtcg ctgccgcgcg aagtcggcaa gcatcccgaa
     1021 agcggcgaag cgatcacggc gaatttcggc cgcttcgggc cttacatcgc ccatgacggg
     1081 aaatacgcct ccctcgaaac gccggaagat gtcttcacca tagggctgaa ccgcgccgtc
     1141 acggtgctgg ccgagaaaaa agcgcgcggc tttgtcaggc gcgggcagga gccgctgaag
     1201 gaactgggcg ccaatccgaa cggcggtgcc ctcatcaaat tgatgaaggg acgtttcggt
     1261 ccctatgtga cggacggctc gatcaacgcg acggttccca gaggcagcga tcccatggcg
     1321 ctcacgctcg aggaagcgct ggccttgatc gccgaacgcg ccgccaaggg ccccgccaag
     1381 aagaagggcg ggcacttcaa gaaggccgta aaggcgaaac cggcagcgga ggcgaaggca
     1441 aagccggaaa agaaaccgaa agccccggcc aagaagaaat ccgcggccaa gcacaagccg
     1501 gcgccggaag ccgccgaata atatgcccag aggaaaacga accaacgcgc cgcccatcga
     1561 caaggcgcgt gtgctcgaaa cgctggccgc gcggccaggc gccaccaagc gcgacctcgc
     1621 gcgcctgctc aacatcaaag gttcggaccg catcgcgctg aagcgcatcc tgaaagagct
     1681 tgaggccgac ggcctgctct caggcaatcg caagcgcggc tactccaagc ccggcaccct
     1741 gcccgaagtc gccgtgctgg aaatcaccgg ccaggatatg gacggagaac tccttggccg
     1801 cccgcaaacc tgggaatcga acgaagagcc gccgcagatc gtcgtcgttc tgggcgaagg
     1861 ggacgcggcg ctcggacgcg gcgagcgcat tctggcgcgg ctgtcgaaaa ccagcgacgg
     1921 ctacgaggcg cgcatcgtca agcggctggg cgccagcgcc catcgcgtgc tcggcgtggt
     1981 gcgggtatcg ggaccgaatg tccgcatcgc gcccatcgac cgcaaaagca aaagcgaatt
     2041 ttctgtcgat gcgcgcgagc gcgccggcgc ggaaaacaac gaactggtgc tggccgaacc
     2101 catcgccggg cgcgccggtg gatttccccg cgcgcggatc gtggaacgcc tcggcagcat
     2161 ggacgcgccc aaggcggtca gcctgatcgc catccatgcc cacggcattc cgaccgactt
     2221 tcccaaagag gccctggatc aagcggcggc ggctacgcca gccgatccgc gcgggcgtac
     2281 cgatttgcgc gccattccgc tggtcaccat cgatcccgaa gacgcgcgcg accacgacga
     2341 tgcggtgtgg gccggtcccg atcccgacca caagggcgga catatctgcc tggtggcgat
     2401 tgccgacgtc gcccattatg tgacgccggg ttcgccgctc gaccgcgaag cctataagcg
     2461 cggcaattcc gtctatttcc ccgaccgcgt ggtgccgatg ctgcctgaaa ctttgtcggc
     2521 cgatctatgc tcgctgaagg aaggcgtgga ccggccgtgc ctcgcggtgc gcatggtgtt
     2581 cgatgcgggc ggcaagaaac gcgcgcacca attcgtgcgc ggggtgatgc gctcggccgc
     2641 gcggctgacc tatgcccagg cgcaaagcgc tttcgacggc aagccggatg cggcgctgtc
     2701 gcctatcgcc aaggaaaccc tggccgctct gtggaaagct tacggagcat tggtgaaggc
     2761 gcgcgagata cgcgatccgc tcgacctcga tctgccggaa cggcgcatcg agatcggggc
     2821 ggacggcaag atatcctcca tcgccttccg cgagcggctg gaatccatgc gcttgatcga
     2881 ggagttcatg gtgctcgcca acgtcgcggc ggcggagacg ctggagaagg cgcgcacgcc
     2941 cttgatctat cgcgtccacg aagagccttc caaggaaaag ctgttcacct tctcggatta
     3001 cctgcgcacc atcggggtga atttcgccaa gggccaggtg atgaaacctg gtgtcttcaa
     3061 ccgcatcctg gcgagcgtca aggatggccc gcacgagaag gtgatgaacg acgtggtgct
     3121 gcgcacccag gcgcaggcga tctatcatcc cgacaatctc ggccatttcg gcttgaacct
     3181 ggcgaaatac gcgcactaca cctcgccgat ccggcgctat gcggatttga tcgtgcatcg
     3241 tgcgctgatc cgggcgctca agctgggtga cggcgggctg acggagcgcg agaccgcgcg
     3301 gctcggcgag atcgccgaac acatctccat gaccgagcgg cgcgccatgg cggcggaacg
     3361 cgattccacc gaccgctacg tcgccgcttt catgcaggac cgcatcggcg cgcgtttcga
     3421 tgcgcgcgtc acgggcgtga cgcgcttcgg cctgttcgtg cgcttggccg agaccggggc
     3481 ggaaggcttg ctgcccgcgc gcgtgctggg cacggaatat ttccgccacg acgaacgccg
     3541 ccatgccctg gtcggcgaac gcaccggcac ggtttacggg ctgggcgcta tcgtcgcggt
     3601 gcggctggcc gaagcggcgc cgctgaccgg cggactgcgg ttcgatctgg cggaggcgga
     3661 gtctgcaccg aagacgcgcc gccctgcggt cgttcgtcag cggagcaagc ggaggcgttg
     3721 aagctgagac gtgttgatca cgccctcggg gcgtttctct ctgcgagggc ctttaggtat
     3781 cgcaggcccc atgcttggta aggaatttct ctagttccgt cacgagtttg gaataatcgg
     3841 acaacgtttc ggccgtcgta tctacgatgc gaccccgcct cactccggaa gtgcccatct
     3901 tgccttcttt catgagtcgg atggcgttgc gccagatttt gcgttgttcc attgcccatg
     3961 aaaacatggg gtcagcgcgt ttcttgcttt gggttccctt gggcatggta agatagtctg
     4021 tcacctcagg agtatattcg gctatgggct ggcttatcgc aatcggcatc gggcagttaa
     4081 ttgtcctcgg ctacatcgcc tacaggatcg agtttctatt acgtggcgac caagccgccg
     4141 cctacacaaa ccttgttctg gagagactca acaacattga cgcttggtac gccgccgaaa
     4201 acgctcgcat caagcgcgat caggaatggg cctaaatttc caactgtgac agtgccccag
     4261 ttttgcccca ccttgacatc ccggacttcc cggctaggtt acgccccctt tgacgcgcct
     4321 atctcaggag tacccccatg gcgaagccaa ccactgccaa gatccggctc aattccgagg
     4381 gcggtacggg ctttttctac gtcaccaaga agaacacgcg caccatgacg gagaagttct
     4441 ccatgaagaa gtacgacccc gtcctgcgca agcatgtcca gttcaaggaa ggcaagatta
     4501 agtagtctgc ctcgttttcg tcatggctgg acgcctccgg cccctgcacc aaccttcgtc
     4561 atggcagccc gccgaagcct ttggcgtagg tgggaggcgg ccattcagtt tttgtgcaat
     4621 cgaagattcc cttgttttcc tggatggccg ggtcaagccc ggccatgacg aattgtagtt
     4681 gatgccgcct ccaccctttc cacgaacggc aggatgaacc cgagcagcag cgggatcacg
     4741 cttggcggca ggtcgtgccc ccagccctcg atctcgtgat attcggcgcc ttggatgcaa
     4801 tcggcgatat gccgtccgtg ggcgcaaggc agcagcgtgt cggccgttcc gtgcagcacc
     4861 agcgccggta gtttcagtcc tttcaggcgt ttgccgcgcg gcgggctggc caggatcgcc
     4921 gcccactggc gcaacccgcc ttcgggatag aagcaacggt cgtaagcgcg gccgatcaac
     4981 tcggccgcct gttcgtcatc ggccgcgaat gcggtggagg cgtaggcacg gcgcgatttg
     5041 agcacatgag cgatcaccgt ggcgcgatcg ttcgcagacg gcggcgtgat cagcgactga
     5101 tgcgcttcgg ccgacgatgg cggcaggccg gcatcgttgg tggtcgtgaa aatctggatc
     5161 agcgagcgcg tcttccgggg gtggtcgagc gccacaagct gcgcaatcat gccgcccatc
     5221 gacgcgccga ggatgtgggc gctggcaata ccgagcgcgt cgagcaggcc ggcggcatcc
     5281 gccgccatgt ccttcaggaa atagggaaca ttcggtttgc gccgttcgcg cgccgccgcc
     5341 atcaccgcgc cgaggtccgg gatctgcccg tcccatttct gtgtcaggcc ggtatcgcga
     5401 ttgtcgaagc ggatgacacg gaggccggcc tgcgccaatc cgtccacgaa ctcgtccggc
     5461 caggcgatca actgctggcc gaaaccatgg atcagcagga tcggcgcgcc atccttaggc
     5521 ccggaaatct cgtattcgat atcgatgcca tttgcgtgga ttccagccat gaagtccccc
     5581 ctttccacta gagcgcaatc cggcaaagtg gtcggtagca gcagcgtgcc gacgcggttt
     5641 gccctcagat tgcgcgaaaa acaaggacac tagcaaccta ttgaatccta gagcgttttc
     5701 aggaaaagtg gaacccggtt ttccgtccga aaacgcgaca aattgagaac ttagatgatc
     5761 gttatcggac cacacccgtt ccttgacaag atgccagccg cgccggagcc tc
//
LOCUS       DASWPU010000430         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_52474,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000430 DASWPU010000000
VERSION     DASWPU010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52474"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VGC27_09260"
     CDS             <1..1129
                     /locus_tag="VGC27_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282797.1"
                     /translation="QAARLMGCARREAAFAALLFMSILLFDYTYAGLNDPHLLGQAIQ
                     IGGLILVLREPRTTASMALAAFIFVAAFFTKHSLIAQPLAAGLWLLAFDRRNGMKLAF
                     FGVAWTAVGIAAFAFAFHTSLWSQLNNPRVWLLRYSTHAMAARAPEGLLPLAASLALL
                     ARFRGDKHVVLCLSYLVVSLLIAAYFLGGAGVGSKALYDAVIALSLCGGIGVNRLAKL
                     RLWGLRARPLYAACHFLPVVGILIYHPVTGTLPPHWLSRGSAPVVDTERDIAFLKSKD
                     GAVLCNAQPLCYWAGKRAEVDLWGYEQAVAVGVRDGHELRDAIRQRRYTVLQLASPPD
                     VLGAPPEVRGAYTESISREIEANYRIDHTSFNGAFWVPRTP"
     gene            complement(1154..2428)
                     /gene="eno"
                     /locus_tag="VGC27_09265"
     CDS             complement(1154..2428)
                     /gene="eno"
                     /locus_tag="VGC27_09265"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY0282798.1"
                     /translation="MTAIIDIAAREILDSRGNPTVEVDVRLEDGSFGRAAVPSGASTG
                     AHEAVELRDGEKRYGGKGVEKAVAAVNGELFDALCGMEAEDQVRLDKAMIALDGTPNK
                     ARLGANAILGVSLATAKAAAEAANLPLYRYVGGAKANLLPVPMMNIVNGGVHADNPID
                     FQEFMIVPAGAPSFREALRYGAEIFHVLKKALKDAGHNTNVGDEGGFAPNLKSADEAL
                     TFIVKAIEKAGYKPGEDVFIALDPASSEFFKDGKYVLAGEGRTLDPLAMVKVYEELCA
                     RFPIFSIEDGLAEDDWDGWIAITKSLGDKVQLVGDDLFVTNTQRLKQGIVKGAANAIL
                     VKVNQIGSLTETLEAVEMAHKAAYRAVMSHRSGETEDSTIADLAVATNCGQIKTGSLC
                     RSDRLAKYNQLLRIEEQLGDQAVYAGKSVLKA"
     gene            2748..3179
                     /locus_tag="VGC27_09270"
     CDS             2748..3179
                     /locus_tag="VGC27_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY0282799.1"
                     /translation="MTSVPADLLSAIVTLLAVILYFYMSVRVSRMRTKYGVKAPATTG
                     HPEFERAFRVHYNTMESLVAFLPLLWLATFYFAPAFYALAWLPPALGLAWIVGRIFYM
                     NGYMAGPDKRGVGFGIASLAQLLLLVLAIVGIVRVWIAIAA"
     gene            complement(3199..4047)
                     /gene="kdsA"
                     /locus_tag="VGC27_09275"
     CDS             complement(3199..4047)
                     /gene="kdsA"
                     /locus_tag="VGC27_09275"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY0282800.1"
                     /translation="MIPVQPNATVKVGSVEIGNAQKLAIIAGPCQLESRAHAFEMAQA
                     LKEITAKAGVGLIYKTSFDKANRTSATTSRGMGLELALPIFADIRRELGVPVLTDVHE
                     RDHCAAVADAVDVLQIPAFLCRQTDLLVAAAKTRKAVNIKKGQFLAPWDMKNVIAKVT
                     GAGNPNVLATERGVSFGYNTLVSDMRSLPVMAKFGVPVVFDATHSVQQPGGLGATTGG
                     DREMVPYLARAAVAVGVAAVFMEVHQDPDTAPSDGPNMLRLKDFPALLDELIAIDAIA
                     KKRMTY"
BASE COUNT          669 a         1371 c         1348 g          855 t
ORIGIN      
        1 gcaagcggcg cgtctcatgg gctgcgcccg gcgcgaggcc gcgtttgcgg cgctgctgtt
       61 catgtccatc ctgctgttcg actacaccta tgccggcctg aacgatcccc atcttctggg
      121 ccaagccatt cagatcggcg gcttgatcct cgtgctgcgc gagccgcgga ccaccgcttc
      181 gatggcgctt gccgcgttca ttttcgttgc cgcgttcttc accaagcaca gcctcatcgc
      241 ccagccgctt gccgccggcc tgtggcttct ggctttcgac cggcggaacg gaatgaagct
      301 cgcgtttttc ggcgtggcgt ggaccgccgt cgggatcgcc gccttcgcct tcgcgttcca
      361 cacttcgcta tggtcgcagc ttaacaatcc tcgcgtctgg ctgctgcggt acagcacgca
      421 tgccatggcg gcgcgcgctc ctgagggatt gcttccgctc gccgcctcgc tcgctttgct
      481 ggcgcggttc cgcggggaca aacacgtggt cctctgccta tcgtacctcg tcgtgtcgct
      541 cttgattgca gcgtatttcc tcggcggcgc gggcgtgggc agcaaggcgc tctacgacgc
      601 cgtgatcgcg ctgtcgctct gcggcgggat cggcgtgaac cggctggcga agctcaggct
      661 gtgggggttg cgggcccggc cgctctacgc cgcgtgccat ttccttccgg tggtgggaat
      721 cctgatctac caccccgtca cgggaacgct gccgcctcac tggctgagcc gcggctccgc
      781 gcccgttgtg gacaccgagc gggacatcgc attcctgaaa tcgaaggacg gagcggtcct
      841 gtgcaacgcg cagccgctct gttattgggc cgggaagcgc gccgaagtgg acttgtgggg
      901 atacgagcag gccgtggccg tgggcgttcg cgacggccac gagctgcgcg acgcgatccg
      961 gcagcgccgc tacaccgtct tgcagctcgc ttcgccgccc gacgtgctag gcgcgccgcc
     1021 cgaagtgcgg ggggcgtata ccgaaagcat ctcgcgtgaa atcgaggcca attaccgcat
     1081 cgaccacacc agcttcaacg gcgccttctg ggtgccgcgg acgccctaac gaaaaaggca
     1141 acgggacgaa gcgttacgcc ttcagaacgc ttttgcccgc gtacaccgcc tggtcgccca
     1201 actgctcttc gatacgcagg agctgattgt acttggccag gcgatccgag cggcacagcg
     1261 atccggtctt gatctgcccg cagttcgtgg cgaccgcgag gtcggcgatg gtggaatctt
     1321 cggtctcgcc cgaacggtgg gacatcacgg cgcgataggc ggccttatgg gccatctcga
     1381 ccgcttccag cgtctcggtc agcgagccga tctggttgac cttgacgagg atcgcattgg
     1441 ccgcgccctt gacgatgccc tgcttcaggc gctgcgtgtt ggtgacgaag agatcgtcgc
     1501 cgacaagctg caccttgtcg cccagcgact tggtgatggc gatccagccg tcccaatcgt
     1561 cttcggccaa gccgtcctcg atggagaaga tgggaaagcg cgcgcaaagt tcttcataga
     1621 ctttgaccat cgccagggga tcgagcgtgc ggccctcgcc ggccagaacg tacttgccgt
     1681 ccttgaagaa ttcggacgac gccggatcga gcgcgataaa gacgtcctcg ccgggtttgt
     1741 agcccgcctt ctcgatggcc ttgacgatga aggtcagcgc ttcgtcggcg cttttcaggt
     1801 tcggcgcaaa accgccttcg tcgccgacat tggtgttgtg accggcatcc ttcagcgcct
     1861 tcttgagaac gtggaagatt tccgcgccat agcgcagcgc ctcgcggaag ctcggcgcgc
     1921 ccgccggcac gatcatgaat tcctggaagt cgatcgggtt gtcggcgtgc acgccgccat
     1981 tgacgatgtt catcatcggc acgggcagca gattggcctt ggcgccgccg acatagcggt
     2041 agagcggcag gttcgccgcc tcggccgccg ccttggccgt cgccagcgac acgcccagaa
     2101 tggcgttggc gcccagccgc gccttgttgg gcgtgccgtc gagcgcgatc atcgccttgt
     2161 cgaggcggac ctggtcttcg gcttccatgc cgcagagcgc atcgaacagc tcgccgttca
     2221 ccgcagcgac cgccttctcg acgcccttgc cgccatagcg cttctcgccg tcgcgcagtt
     2281 ccaccgcctc gtgtgcgccc gtggaggccc cggacggcac ggcggcgcgg ccgaacgagc
     2341 cgtcttccag cctgacgtcc acttccacgg tcggattacc gcggctatcc aggatttcgc
     2401 gggcggcgat gtcgatgatt gcggtcattt tgcttttcct tcgtcagttc ggtgcgcctg
     2461 ctttaagcga taccaagtac tccatcaagc gcgacggagt ggggcaaatt cgacgtataa
     2521 ccccgaaaag gaacaacatc atgtcgtttt cccgagtaat tttgtcggcg ttcgccgcgg
     2581 ctttgctgtc caccgcatct tttgccgccg accggccatc cgaggatgga ccgcggcgcg
     2641 tccggccaag gcggcggcca gtaacccagg ccgtgcgaat cctgtaagtt gcggcaaagg
     2701 ttcccgcgcg attcccgtgc ggcagccgtc actttgggga aacgccaatg acctccgtac
     2761 ccgcagacct gttgagcgcg atcgtcacgc ttttggccgt tatactctat ttctatatga
     2821 gcgtccgcgt cagccggatg cgcacgaagt atggcgtgaa ggcgccggcg accacaggcc
     2881 atcccgaatt cgaacgcgcg tttcgtgtcc actacaacac gatggaatcg ctggtcgcgt
     2941 tcctgccgct gctgtggctg gcgacgttct atttcgcgcc cgccttttac gcgctggcgt
     3001 ggctgcctcc ggcgctcggc cttgcctgga ttgtcggacg gattttctac atgaacggct
     3061 acatggccgg tcccgacaag cgcggcgtcg ggttcggtat cgcgtctctg gcccagcttc
     3121 ttctgctggt cctcgccatc gtaggtatcg tgcgggtctg gatagccatc gccgcctgag
     3181 cgggagacga agcccgattc agtacgtcat gcgcttcttg gcgatggcgt cgatggcgat
     3241 gagttcgtcg agcagcgccg ggaagtcctt caggcgaagc atgttggggc cgtcggatgg
     3301 cgccgtgtcc gggtcctgat gcacttccat gaaaacggcg gcaacgccga ccgccacggc
     3361 ggcgcgcgcc agatagggca ccatctcgcg gtcgccgccc gtggtcgcgc ccagcccgcc
     3421 cggctgctgc accgaatgcg tcgcgtcgaa caccaccggc acgccgaatt tcgccatgac
     3481 cggcagcgac cgcatgtcgg agaccagcgt gttgtagccg aagctcaccc cccgctcggt
     3541 cgccagcacg ttgggattgc ccgcgccggt gaccttggcg atcacgttct tcatgtccca
     3601 gggcgcgagg aactggccct tcttgatgtt gacggccttg cgggttttcg cggcggcgac
     3661 caagagatcg gtctggcggc aaaggaacgc cggaatctgc aacacatcca ccgcatcggc
     3721 gacggcggcg caatggtcgc gctcatgcac gtcggtcagc acgggaacgc cgagttcgcg
     3781 gcggatgtcg gcgaagatgg gcagcgccag ttccagcccc atgccgcgcg aggtggtggc
     3841 cgaggtccgg ttggctttgt cgaaactggt tttgtagatc agaccgacgc cggctttggc
     3901 cgtaatctct ttcagcgcct gcgccatttc gaaggcgtgc gcccggcttt cgagctgaca
     3961 gggaccggca atgatggcga gcttttgcgc gttgccgatt tcgacggaac cgaccttgac
     4021 ggtcgcattg ggctggacag ggatcatgcg tccattctag cgcagggttc gttgagatgg
     4081 aaacgtctgt cattcccggc cgaccccgga cttgatccgg gggaggggaa ggaaatccat
     4141 acgcctgtgc agtttctgga ttcccttccc tcgccgggaa tgacaatgac ggccgtgact
     4201 tctatttcag caaatacgcc gcggtcgcga agggcgagat gat
//
LOCUS       DASWPU010000431         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_71466,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000431 DASWPU010000000
VERSION     DASWPU010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71466"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VGC27_09280"
     CDS             complement(<1..966)
                     /locus_tag="VGC27_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0282801.1"
                     /translation="MKSNRPTPPPPESRPSKRPANIYDVAKGANVSIKTVSRVVNGLS
                     NVSEATRKRVMETVEALHYRPNVFARGLASEKSFMIGLLCDIPGAGSPYLAELQTGVL
                     SRCRKDGYHLIVESLDAEDPNLGKHVHSLVSASNLRGIILTSPLCDVPAVMEALDRAE
                     TPFVRIAPEKPLAGLFDVRIDDRKAAYDMTAYLIGLGHKRIGFIKGPPDHGDANARFE
                     GYCAAIAHAGLPKVEELCVQGRFTYQSGLEAGERLLSLKKRPTAIFAGNDAMAAAVLA
                     TSQRFNLKIPLELSLAGFDDSLFAQIVWPRLTTCRQPVEEMAKVAV"
     gene            complement(1030..1920)
                     /locus_tag="VGC27_09285"
     CDS             complement(1030..1920)
                     /locus_tag="VGC27_09285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0282802.1"
                     /translation="MNGSRDIDTFSYASGDDARLKRLVIRLIERMTGQPYLRWLYEDY
                     RSRPHAGESFWDSAIRRLELNVTCDEAKLAAWPKTGPLVVVSNHPFGVLDGVVICHLI
                     AKVRTDFRVLTNSVLLRAEEIRPFMLPIDFAVNTQARKTNLQSRADAKAHLVGGGCLV
                     IFPAGGVSTTPTVWHRRAVDADWKTFTARMITQARAPVAPVFFAGQNSRLFQLVSHIS
                     LTLRLSLLFKEVHDKIGGEVRVRVGDVVPYDALASITDRYAFMQRLRELTYALGSGVQ
                     NPPKPHLKRPRRAPKHGRSS"
     gene            complement(1917..>2584)
                     /locus_tag="VGC27_09290"
     CDS             complement(1917..>2584)
                     /locus_tag="VGC27_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282803.1"
                     /translation="YQSIAQLRQHIRSAERIHGIISEGGLAPNVVPERAACRFFVRAA
                     DADDLAPLKARVQACFEAGAIATGCRLELKWGGADYLDLKTNWPMAEVFERQAQALGR
                     EFFPMKDISPGFSGSTDMGNVSHRVPSIHPMLAVAPAGVIIHNPEFARWAASERGDAA
                     VIDGAKAMALTALELMSDGKLMNIVRGDFEATAELSRLAVSKTGEVPPIQNPHAHGNC
                     GCT"
BASE COUNT          470 a          783 c          862 g          469 t
ORIGIN      
        1 tacggccact tttgccatct cctcgaccgg ctgacggcag gtcgtgagcc tcggccatac
       61 gatttgggcg aaaagagaat cgtcgaagcc ggccagggat aattctagcg ggattttcag
      121 gttgaaacgc tgcgaggtag ccagcacggc ggcggccatg gcgtcgttgc cggcgaagat
      181 cgcggtagga cgctttttca gcgacaagag ccgttcgccc gcctccaaac ctgattgata
      241 agtgaacctg ccctgcacac agagttcttc gactttgggc aagccggcat gggcgattgc
      301 tgcgcaatac ccctcgaagc gcgcgttggc gtcgccgtga tcgggagggc ctttgatgaa
      361 tccgatccgc ttgtggccga ggccgatcag ataagcggtc atatcatacg ccgccttgcg
      421 gtcgtcgatg cgcacgtcaa acaaacccgc cagaggcttt tccggcgcta tgcgcacgaa
      481 tggcgtttcg gcgcggtcaa gcgcctccat caccgccggc acgtcgcaaa gcggcgacgt
      541 caggatgatg ccgcgcaggt tcgacgcgga aacaagcgag tgcacgtgct tgccgaggtt
      601 gggatcctcg gcatcgaggg actcgacgat cagatggtag ccgtccttgc ggcaacgcga
      661 gagcacgcca gtctggagtt cggccaggta gggcgatccc gcccccggaa tgtcgcacag
      721 caatccgatc atgaacgatt tttcgctggc caatccgcgc gcgaaaacgt tgggccggta
      781 gtgcaaggct tccacggtct ccatgacgcg cttgcgtgtt gcttcgctga cgttcgacag
      841 cccgttgacg acgcgcgaga cggttttgat ggaaacgttg gcgcctttcg ccacgtcgta
      901 gatgttagcg ggcctcttcg aggggcggct ttcgggaggc ggcggggtgg gacgattcga
      961 tttcacggag gaattctagg ccaggatcgc gtccggtaaa agcacgacaa aaaagcactg
     1021 gaaatagcgt taagaagaac ggccatgctt gggcgcacgc ctcggccgct tcaggtgcgg
     1081 cttgggcggg ttctgcacgc ctgaaccgag tgcgtaggtc aactctcgca gccgttgcat
     1141 aaaggcgtag cggtcggtga tggaagccag cgcgtcgtag ggcacgacgt cgccgacgcg
     1201 cacgcgcacc tcgccgccga tcttgtcatg gacttccttg aacagcagcg acaggcgcag
     1261 cgtcaggctg atatggctca ccagctggaa caggcggctg ttctgaccgg cgaagaacac
     1321 cggggccacc ggcgcgcgcg cctgggtgat catccgcgcg gtgaaggtct tccagtccgc
     1381 gtctaccgcc cgcctgtgcc agacggtcgg tgtggtcgaa acgcccccgg ccggaaagat
     1441 cactaggcag ccgccgccca cgagatgcgc cttggcgtcg gcgcgcgatt gcaggttcgt
     1501 cttccgcgcc tgtgtgttca cggcaaagtc gatgggcagc atgaacggcc tgatttcttc
     1561 ggcgcgcagc aggaccgagt tcgttaacac ccggaaatcg gtcctgactt tcgcgatcag
     1621 gtggcaaatc acaaccccgt cgagaactcc gaaaggatgg ttcgagacaa cgaccagcgg
     1681 ccccgtcttc ggccacgcgg ccagtttcgc ctcgtcgcag gtgacgttga gttcgaggcg
     1741 gcggatggcc gaatcccaga agctctcgcc cgcgtgcggg cggctgcgat aatcctcgta
     1801 gagccagcgc agataaggct ggcccgtcat gcgctcgatc aggcggatga cgagtcgctt
     1861 cagccgcgca tcgtccccgg aggcatagga aaaggtgtcg atgtcgcgcg atccgttcac
     1921 gtgcagccgc aattgccgtg ggcatgagga ttttggatcg gcggcacttc gccggtcttg
     1981 gagacggcca gcctggacaa ttcggccgtc gcttcgaaat cgccgcgcac gatattcatc
     2041 agcttgccgt cgctcatcag ttcgagggcg gtcagcgcca tggccttggc gccgtcgatg
     2101 acggcggcgt cgccccgttc cgaagcggcc cagcgggcaa actccggatt gtggatgatg
     2161 acgccggccg gcgccacggc gagcatgggg tggatcgacg gcacccggtg gctgacgttt
     2221 cccatgtcgg tcgagccgga gaagcccggc gaaatgtcct tcatcggaaa gaactcgcgg
     2281 cccagcgcct gcgcttggcg ttcgaagacc tcggccatcg gccagttggt cttgagatcg
     2341 agatagtccg cgccgcccca tttgagttcg aggcggcagc cggtggcgat ggcgccggcc
     2401 tcgaagcagg cctgtacccg cgctttcagc ggcgccagat cgtcggcgtc ggcggcgcgc
     2461 acgaagaagc ggcaggccgc gcgctccggc acgacgttcg gcgccaggcc gccttcggag
     2521 atgatgccgt ggatgcgctc ggcgctgcgg atgtgctggc gcaattgcgc gatcgactga
     2581 tagg
//
LOCUS       DASWPU010000432         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_22398,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000432 DASWPU010000000
VERSION     DASWPU010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22398"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VGC27_09295"
     CDS             complement(<1..387)
                     /locus_tag="VGC27_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282804.1"
                     /translation="MSNAGWGEIWRLVLAQYGAQPIHLRILIGLGIAFVILMIVEGLR
                     VSFVSGRRLPALPHGVAATPPLKTAVKKTAGAEAASFAASSGPFRPRVIVLTYTPKRT
                     KGHVSRHCPARPKLRRFPGELAMSHQP"
     gene            complement(536..1579)
                     /locus_tag="VGC27_09300"
     CDS             complement(536..1579)
                     /locus_tag="VGC27_09300"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY0282805.1"
                     /translation="MMMMDLAVPLLRLLPPEPAHRLTVELAARFGAFLPRAPKDDPRL
                     AVRALGLSFPNPVGVAAGFDKNARAFRTMLKAGFGHMECGTVTPLPQPGNPRPRLYRL
                     SEDGAVVNRMGFDNEGMERIGERLARRRKGIVGINIGANKDSTDRIADYRACFARLSP
                     HADYVAVNVSSPNTPGLRGLQEREQLHALLATLVEARAGKKVPLLLKIAPDLDARALD
                     DIAAEVLASGIEGLIVTNTAIARPPGLKSRYAEEPGGLSGRPLFKPSTWVLAEMRKRV
                     GAKLVLVGVGGIASGADAYAKIRAGATLIQLYTALVYDGAGLLVRIKRELLECLARDG
                     FAAISDAVGADVK"
     gene            1787..2422
                     /locus_tag="VGC27_09305"
     CDS             1787..2422
                     /locus_tag="VGC27_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0282806.1"
                     /translation="MLTHKQIWSAIDALAAKNRLSSSGLAKLSGLDPTTFNKSKRGTP
                     TGKLRWPSTESVSKVLAATGTSLEDFVALVSRDGGTRRQVRLVPVVGMAQAGSRGFFD
                     DAGFPAGAGWDEIPFPELADEHAYALEITGESMLPVYRDGDRIIVSPAANVRRGDRVV
                     VKTNAGEVMAKLLTRLTAQKVELKSLNPAYDDRSFALSEVAFVHRIIWASQ"
     gene            complement(2480..3463)
                     /locus_tag="VGC27_09310"
     CDS             complement(2480..3463)
                     /locus_tag="VGC27_09310"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0282807.1"
                     /translation="MTNQPADDGGSAILRPSRRTTLLGAAAVAATVSLLPASAAPRRP
                     GHATPQGESTMSSIKTKDGTEIFYKDWGTGQPVVFSHGWPLTADDWDAQMLFFLAHGY
                     RVIAHDRRGHGRSSQTATGNDMDTYADDLAALTAALDLKNAIHIGHSTGGGEVARYLG
                     RHGASRVAKAVLVGAVPPLMLKTDANPGGLPREVFDGFRAQVAANRAQFYQDVPIPFY
                     GFNRPGAKVSEGIKQNWWRQGMMGSIKSHYDCIKAFSETDFTEDLKKIDVPTLILHGD
                     DDQIVPIGASALLSAKLVKNATLKVYPGFPHGMCTTQPEVLNADLLAFAKA"
     gene            complement(3572..>4160)
                     /locus_tag="VGC27_09315"
     CDS             complement(3572..>4160)
                     /locus_tag="VGC27_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282808.1"
                     /translation="RSRDKPCLECLERAEDFARRAIAADPKRPDARIYLAFSLGLKAH
                     IVGSLVARLKNYPEQAKHALDVALAADPKNAWALAARGSWNIAIVRGGGKALARLIYG
                     ATLKKGLDDFAAAFDAAPDNIVVLYQYALTLSEYDPVHYRDYIAAALTHVASRTSDTA
                     YERLMQKRAGELLELLKKGDRAAYEARVRKYQGYP"
BASE COUNT          754 a         1329 c         1345 g          732 t
ORIGIN      
        1 cggttgatgg gacattgcta attcgccggg aaagcggcga agtttcgggc gcgcggggca
       61 atggcggctg acatggcctt tcgttcgctt tggggtgtaa gtgaggacga tcacacgcgg
      121 acgaaacggc ccggacgatg ccgcaaaaga cgcggcctcg gcgccggccg ttttcttgac
      181 ggctgtcttc aagggcgggg ttgccgccac gccatgcggc agcgccggca agcggcgtcc
      241 gctaacgaaa ctgacacgca gaccttccac gatcatcagt atgacaaagg cgattcccag
      301 tccgatcaaa atccggagat gaatcggctg ggcgccgtac tgcgccagca caagcctcca
      361 gatttcaccc catccggcat tactcattgc cgactgcccc caaccccgaa cctttttcca
      421 ttgtgcataa aacagacaga tttcaaaagc tttcctgcgc gctgaaacat cgcgtgccga
      481 ttccgtctcg gctgcacagg aagcaggcag ggcgattagt tattgggcgt ggccgctact
      541 tcacatccgc tccgacggcg tcggagatgg cggcgaagcc gtcgcgcgcg agacattcca
      601 gcagttcgcg cttgatgcgg acgagcaggc cggcgccgtc gtaaaccagc gcggtataaa
      661 gctggatcag cgtcgccccg gcgcggatct tggcataggc gtcggcgccc gaagcgatgc
      721 cgccgacgcc gaccagcacg agttttgctc ccacccgctt gcgcatttcg gccagcaccc
      781 aagtggacgg cttgaacaac ggcctgccgg aaagcccgcc cggctcctct gcgtaacggc
      841 tcttcagacc gggcgggcgc gcgatggcgg tgttggtgac gatcagccct tcgatgccgg
      901 aagccagaac ctcggcggcg atatcgtcca gcgcgcgcgc gtcgagatcg ggcgcgatct
      961 tgagcagcag cggcaccttc ttgccggcgc gcgcttcgac cagcgtggcc agcagcgcgt
     1021 gcaactgctc gcgctcctgc aagccgcgca ggcccggcgt gttcggcgag gagacgttga
     1081 cggcgacgta atcggcgtgc ggcgaaagcc gcgcgaagca cgcgcggtaa tcggcgatgc
     1141 ggtccgtgct gtccttgttg gcgccgatgt tgatgccgac gatgcctttg cggcggcgcg
     1201 ccaagcgttc gccgatgcgc tccatgccct cattgtcgaa gcccatgcga ttgacgacgg
     1261 cgccgtcctc gctgagccgg taaagccgcg ggcgcggatt gccgggctgc ggcagcggcg
     1321 tgaccgtgcc gcattccatg tgcccaaaac cggccttgag catggtgcgg aaggcgcgcg
     1381 cattcttgtc gaacccggcg gccaccccga ccggattggg gaaagaaagg cccagcgccc
     1441 ggacggccag gcgcgggtcg tccttgggcg cgcgcggcag aaacgcgccg aatcttgcgg
     1501 ccagctccac cgtgaggcgg tgggcgggtt cgggcggtaa aagacgcaga agcgggacgg
     1561 caaggtccat catcatcatg acaggcctct agaccgaccc ccaccgagag tggtaattcc
     1621 tattgttaac acaatatagg aacaattacc gtccatcgga tcgctttctt agcgtttctc
     1681 acggcagcgc ggtacgagtc gccgcctcac cgatccgacc atgtaagagg acgcacgcat
     1741 agaagcgatt tgaagcgaac cgcaaacgat ccaacggaat cgaacgatgc tgacccataa
     1801 gcaaatttgg agcgccatcg acgcactggc cgccaagaac agactgtcgt cttcgggcct
     1861 ggcaaaactc tcgggcctcg atcccaccac tttcaacaag agcaagcgcg gtacgcccac
     1921 cggcaagctg cgctggcctt ccaccgaaag cgtttcgaaa gtgctggcgg ccacgggcac
     1981 cagcctggag gatttcgtgg cgctggtcag ccgcgacgga ggcacgcgca ggcaggtgcg
     2041 gctggtgccg gtcgtcggca tggcccaggc cggcagccgc ggcttcttcg acgacgccgg
     2101 atttcccgcc ggcgcgggct gggacgaaat ccccttcccc gaactcgccg acgagcacgc
     2161 ctatgcgctg gagatcaccg gcgagtccat gcttccggtc tatcgcgacg gcgacaggat
     2221 catcgtctcg cccgccgcca atgtgcgccg cggcgatcgc gtggtggtga agaccaacgc
     2281 aggcgaagtg atggccaagc tgctgacgcg gctgaccgcg caaaaggtgg agctgaagtc
     2341 gctcaatccc gcctacgacg accgctcctt cgcgctgtcc gaggtcgctt tcgtccaccg
     2401 catcatctgg gcgagtcagt gacgggccgc gcccccgtcc tcggccgggg ttcgcgcacg
     2461 aagcacacga gctaactcgt cacgctttcg cgaaagcgag cagatcggcg ttcagcacct
     2521 ccggctgcgt cgtgcacatg ccgtgcggaa aaccgggata gactttcaac gtcgcgtttt
     2581 tcaccagctt ggccgagagc agggccgagg cgccgatcgg cacgatctga tcgtcgtcgc
     2641 catgcaggat cagggtcggc acgtcgatct tcttgaggtc ttcggtgaaa tcggtttccg
     2701 aaaacgcctt gatgcagtcg tagtggcttt tgatcgatcc catcatgccc tgacgccacc
     2761 aattctgctt gatgccctcg gacactttgg cgcccggccg gttgaagccg taaaacggga
     2821 tcggaacgtc ctggtagaat tgcgcccggt ttgccgccac ctgggcgcgg aagccgtcga
     2881 acacctcgcg cggcagcccg ccgggattgg cgtcggtctt cagcatcagc ggcggcacgg
     2941 cgccgaccag caccgccttg gcgacgcgcg atgcgccgtg gcggccgaga taacgcgcca
     3001 cttcgccgcc gccggtggaa tggccgatat ggatggcgtt tttgagatcg agcgccgccg
     3061 tcagcgccgc cagatcgtcg gcataggtat ccatgtcgtt gccggtcgcc gtctggctcg
     3121 aacggccatg gccgcgccgg tcatgggcga tcacccgata gccgtgcgcc agaaagaaca
     3181 gcatctgcgc atcccagtcg tcggcggtga gcggccagcc atgcgagaac acgaccggct
     3241 gtcccgtgcc ccaatccttg tagaaaatct ccgtgccgtc tttcgtcttg atggagctca
     3301 tcgtggattc tccttgtggg gtggcgtgac ccggccggcg cggtgccgcc gaggccggaa
     3361 gaagggaaac cgttgcagcc accgccgccg cgccgagcag cgtggtgcgc cgtgacgggc
     3421 gaaggattgc cgatccgccg tcatctgccg gttggttggt cattacgtgt ctctttttgc
     3481 agtaaaaatc ttcactgccg aaggggctgc aaacgctctt ccgacgatca atgtagaatg
     3541 attgtaacac acaaacgatg gctgcgggaa gttagggata cccttggtac ttccgtaccc
     3601 gcgcctcgta ggccgcgcgg tcgcctttct tgagcagctc cagcaactcg ccggcgcgtt
     3661 tctgcatcag gcgctcatag gcggtgtcgc tcgtgcggct cgcgacatgc gtcagcgccg
     3721 ccgcgatata gtcgcggtaa tggacggggt cgtattccga gagcgtgagc gcatactgat
     3781 agagtacgac gatgttgtcc ggcgcggcgt cgaaggcggc ggcgaagtcg tccagccctt
     3841 tcttgagagt cgcgccatag atcaggcggg ccaaggcctt gccgccgccg cgcacgatcg
     3901 cgatgttcca gctgccgcgc gcggccagcg cccaggcgtt tttcggatcg gccgccagcg
     3961 ccacgtcgag cgcgtgcttg gcttgctccg gataattctt cagccgcgcc accagggaac
     4021 caacgatgtg cgccttgagg ccgagcgaga aggcgagata gatccgcgcg tcgggtcgct
     4081 tgggatcggc ggcgatggcg cggcgcgcga aatcttcggc ccgctccaga cattcgaggc
     4141 aaggcttgtc gcgcgagcgc
//
LOCUS       DASWPU010000433         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_89172,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000433 DASWPU010000000
VERSION     DASWPU010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89172"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VGC27_09320"
     CDS             <1..470
                     /locus_tag="VGC27_09320"
                     /inference="COORDINATES: protein motif:HMM:NF045129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282809.1"
                     /translation="AFAAKLERLEHGRGRLRRLNLTPNEAAKHGIAVRLDGTRRSAFE
                     LLSLPDVDFPALAGIWPELHGLGPETVEQLEIDARYSGYLDRQEADILAFRRDEALSL
                     PEALDYSAVCGLSTEAAQKLAAIRPATLGQASRIDGVTPAALTLVLAHVKSSA"
     gene            523..1095
                     /gene="rsmG"
                     /locus_tag="VGC27_09325"
     CDS             523..1095
                     /gene="rsmG"
                     /locus_tag="VGC27_09325"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY0282810.1"
                     /translation="MARLKAYVGLLTDWNERHNLVSRASLGGVWQRHLLDSAQLAPLI
                     PATAKTLADLGSGAGFPGLILALLLQGQVAVTLFEATRKKAEFLNAAAERLGLAVTVR
                     NQRIEEAARARFDVVTARACAPLPELLEYAQHFAGRGTVCLFLKGQNVGLELTEARKS
                     WRMKVREHPSVTHPFGVILEIRELDHERNR"
     gene            1079..1891
                     /locus_tag="VGC27_09330"
     CDS             1079..1891
                     /locus_tag="VGC27_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY0282811.1"
                     /translation="MSETVKRPPRVLVVANQKGGVGKTTTAINLGTALAAVGEPTLIV
                     DVDPQGNASTGLGIPRHERKITTYDVLMGQAHLGEAVMPTRIPRLSLVPSTVDLSGAE
                     LELIDVARRNYRLKDALAEYSAHGSSSFSYVLVDCPPSLSLLTVNAMVAADAVVVPLQ
                     CEFFALEGLSQLLKTIELVRTNLNPGLEIQGIVLTMFDKRNKLSDQVAADVRQNMGDM
                     VYNTMIPRNVRVSEAPSHGLPALVYDLRCPGSQAYIKLAGELIQRERMRAAA"
     gene            1888..2778
                     /locus_tag="VGC27_09335"
     CDS             1888..2778
                     /locus_tag="VGC27_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY0282812.1"
                     /translation="MSAEAGKDLAEETRPRGLGRGLSALIGEEAVPTRGEARDRAPRS
                     MPTAFLKPNRFQPRKRFAEEDLNDLVQSVKEKGILQPILVRPVAGESNSFEIVAGERR
                     WRAAQLAKLHDVPVMVRELSDGEALELAIIENVQRSDLNALEEAAAYHELMDRFAYTQ
                     DKVASEVGKSRSHVANTLRLLKLPESVKTMLRDGKLTAGHARTLIGVADPEARAREIL
                     AGALNVREAEQRSKRKKGKTHKQPVSDPNIKALEQSISNGLGLKVQILHKGDKGGEVR
                     ISYKSLEQLDDLTHRLKNGR"
     gene            complement(2824..>3179)
                     /locus_tag="VGC27_09340"
     CDS             complement(2824..>3179)
                     /locus_tag="VGC27_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY0282813.1"
                     /translation="ADARGGRIATQGWAETTELTPAELGERFEDAGVAAILFTDVDRD
                     GLLHGVNVRATAALARSINIPVIASGGVSGLADIEALRAAEEPNISGVVIGRALYDGK
                     IDAKTALDLVNSVRK"
BASE COUNT          609 a         1000 c         1024 g          546 t
ORIGIN      
        1 ctgccttcgc cgctaagctg gagcggctgg agcacgggcg ggggcggctg cggaggctga
       61 acctgacccc gaacgaagcg gcgaagcacg gcattgcagt ccgcttggac gggacgcggc
      121 ggtcggcttt cgagctgctc agcctgccgg acgtggattt cccggcgctg gcgggaatct
      181 ggccggaatt gcacggcctc gggccggaaa cggtggaaca gcttgagatc gacgcgcgct
      241 attctggtta tctggaccgg caggaggccg acatcctggc gtttcgccgc gacgaggcac
      301 tgagcttgcc ggaggcgctg gattactccg ccgtctgcgg cctatccact gaagcggcgc
      361 agaagctggc cgcgatccgg ccggccacac tggggcaggc gtcccgcatc gatggtgtca
      421 cccccgccgc cctgaccctg gtgctcgccc atgtcaaatc cagcgcctga ggccttcgga
      481 cccgaggaat tcgccgccgc aaccggtgtt tcacgtgaaa cattggcgcg gctgaaggct
      541 tatgtgggcc tgctgacgga ttggaacgag cgccataatc ttgtctcccg agcgtctttg
      601 ggcggggtct ggcaacggca tctgctcgac agcgcccagc tcgcgccgct gatcccggcc
      661 acggccaaga cgctggccga cctcggcagc ggggcgggtt ttccgggctt gatcctggcc
      721 ctcctgctgc aagggcaggt ggcggtgaca ttgttcgaag ccacaaggaa gaaggcggag
      781 tttctgaacg ccgccgccga acgcctgggc cttgccgtta ccgttcgcaa tcagcggatt
      841 gaggaggcgg caagggcgag gttcgatgtc gtgaccgcgc gggcctgtgc gcccttgccg
      901 gaattgcttg aatatgcaca gcattttgcc ggccggggca cggtttgcct attcctcaag
      961 ggccaaaatg tagggctcga attgaccgag gcccgtaaat cttggaggat gaaagtccgg
     1021 gagcatccca gcgtgacgca tccattcggg gttattctgg aaatccgaga gttggaccat
     1081 gagcgaaacc gttaagcgtc cgcctcgcgt gctggtggtc gccaaccaga agggcggcgt
     1141 gggcaagacc accacggcca tcaatctggg aacggcgctg gcggcggtcg gcgagccgac
     1201 gctgatcgtc gatgtcgatc cgcaaggcaa cgcctccacc ggactgggca tcccgcgcca
     1261 cgaacgcaaa atcaccacct acgatgtctt gatggggcag gcgcatctgg gcgaggcggt
     1321 catgcccacg cgcatcccgc ggctgtcgct ggtgccttcc acggtggact tgtcgggcgc
     1381 cgaactcgaa ctgatcgacg tggcgcggcg caactaccgg ctcaaggacg cgctggcgga
     1441 atattcagca cacggatcat cttcgttttc ctatgtgctg gtcgattgtc cgccgtcgct
     1501 cagcctgctc acggtcaacg cgatggtggc ggccgacgcg gtggtggtgc cgctgcaatg
     1561 cgagttcttc gcgctggaag gattgagcca attgctcaag accatcgagc tggtgcgcac
     1621 gaatttgaat ccggggctgg aaatccaggg catcgtgctg accatgttcg acaagcgcaa
     1681 caagctctcc gaccaggtcg ccgccgacgt gcgccagaac atgggcgaca tggtctacaa
     1741 caccatgatc ccgcgcaacg tgcgcgtatc ggaagcgccg agccacggcc tgccggcgct
     1801 ggtctacgat ctcagatgtc cgggcagtca ggcctatatc aagcttgcgg gcgaactcat
     1861 ccagcgcgaa cggatgcggg cggccgcatg agcgcggagg cagggaaaga tttggcggag
     1921 gagacgcgtc cgcgcggcct gggccgcggc ctgtcggcct tgatcggcga agaagcggtg
     1981 ccgacgcgcg gcgaagcgcg cgaccgcgcc ccgcgctcca tgccgaccgc gttcctcaag
     2041 cccaaccgct tccagccgcg caagcgtttc gccgaagagg atttgaacga cctcgttcag
     2101 tcggtgaagg agaagggcat tctccagccg atcctggtgc gtcccgtcgc cggcgagagc
     2161 aacagtttcg aaatcgtggc gggtgaacgg cgctggcgcg ccgcccaact cgccaagctg
     2221 cacgacgtgc cggtgatggt gcgcgagctc agcgacggcg aagcgctcga actggccatc
     2281 atcgagaatg tccagcgctc cgatctcaac gccctcgaag aggcggcggc ctatcacgag
     2341 ctgatggacc gcttcgccta cacccaggac aaggtggcga gcgaagtcgg caagagccgc
     2401 agccatgtgg cgaacacgct gcgcctgttg aagctgcccg aaagcgtcaa gacgatgctg
     2461 cgcgacggca agctgaccgc cggccatgcg cggaccttga tcggcgtggc cgaccccgaa
     2521 gcccgcgcgc gtgagattct ggccggcgcc ctcaacgtcc gtgaggccga acagcgttct
     2581 aaaagaaaaa agggaaaaac ccacaaacag cctgtgagcg acccgaacat caaagcgcta
     2641 gagcaaagta tttccaatgg gttggggctg aaagttcaga tcctgcacaa aggggataaa
     2701 ggcggcgagg tgcggatttc ctacaaatcg ctggagcagc tcgacgattt gacgcaccgt
     2761 ctcaagaacg gccgataact tcttaatttt actaattatt aaatataatc gacaaggctc
     2821 gctctatttc cgaacactgt tcactaagtc taacgccgtt tttgcatcga tcttcccgtc
     2881 atacagcgcc cgcccgatca cgacccctga gatattcggt tcttccgccg cccgcagggc
     2941 ctcgatgtcc gccaggcccg acacgccgcc agaggcaatg accggaatgt tgatggaccg
     3001 ggccagcgcc gccgtcgccc ttacattcac gccatgcagc aagccgtccc gatcgacatc
     3061 ggtgaacagg atcgcggcca cacccgcgtc ctcgaagcgc tcgcccagct cggcaggcgt
     3121 cagttccgtg gtctcagccc agccctgggt ggcgatccgg ccgcccctgg cgtcggcgc
//
LOCUS       DASWPU010000434         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_120259,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000434 DASWPU010000000
VERSION     DASWPU010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120259"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VGC27_09345"
     CDS             <1..427
                     /locus_tag="VGC27_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0282814.1"
                     /translation="AVREALARCDAGAFALRRMDELSSGERARVLFARALATGAPILL
                     ADEPAAFLDPAHQLRLMELLREEARRGAAVAVTLHDLPLASRHCDEIVVLHQGRLAAD
                     GTPAAALSDEALAQVFGIAALRVPDPEGGRDALASFRRI"
     gene            526..1299
                     /gene="ppk2"
                     /locus_tag="VGC27_09350"
     CDS             526..1299
                     /gene="ppk2"
                     /locus_tag="VGC27_09350"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY0282815.1"
                     /translation="MRDGEDGGLGAHYSRALRELQIELVKLQRHLIEEGARVLVILEG
                     RDGAGKDGTVKRLIEHMSPRETIVHAPGKPSDRDLTEWYFQRFVPNLPAGGEFVVFNR
                     SWYNRAGVERVMGFSTKQQVKEFLHAVVPFEDMLVRDGLHLRKYYLDISKSEQKKRLS
                     DRHKDPLKQWKSSPVDDAALKNWKSYSKARDEMFKRTSHPHAPWKVVIANDKKIARLE
                     FLRDLLASFDYPGKDKKLSRPDRSIVFDWSEKKRKLLAD"
     gene            1632..2516
                     /locus_tag="VGC27_09355"
     CDS             1632..2516
                     /locus_tag="VGC27_09355"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0282816.1"
                     /translation="MRNSRLHRFWPAVFRRLGLARRLNYDHPLTISGRAFSIPIAGGI
                     GEDLLKLTPDFKSEIIRLFGDRIPNCFVDVGANLGQTVIEAFATRRWDRYFAVEPNPT
                     ACAYLESLVKANHLPVDVLPWGAGADASPHSFCFQGTTDASATMAPEERPGAYSPEMS
                     RSVATYPLDVLLDIFPLPPGLMIKIDVEGFEAEVLAGARNILSDVRPVILCEVLRAYE
                     PASLTFTEKRMSKLEAILAAVDYRIFYLELENEVSGRILSMKEIASFPRALWRESPSG
                     MDYLFVPAEMTLPDFLAG"
     gene            complement(2552..2628)
                     /locus_tag="VGC27_09360"
     tRNA            complement(2552..2628)
                     /locus_tag="VGC27_09360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2592..2594),aa:Arg,seq:cct)
     gene            2784..>3060
                     /locus_tag="VGC27_09365"
     CDS             2784..>3060
                     /locus_tag="VGC27_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282817.1"
                     /translation="MKPNSLAVLAVFLLAPFPANAGANDDAFAAIGKCAAVTEDAARL
                     ACYDKIAGRMKAVEAAPQTAAQAQPQAAAIPAQTAAAAPPAAKEDQEY"
BASE COUNT          591 a          919 c          943 g          607 t
ORIGIN      
        1 tgcggttcgt gaagccttgg cgcgctgcga cgcgggggct ttcgcgctcc gccgcatgga
       61 cgagctttcg tccggcgagc gggcgcgcgt tctgttcgcc cgcgcgctgg ccaccggtgc
      121 gccgattctc ttggccgacg aaccagcggc attcctcgat cccgcccatc agcttcgcct
      181 gatggagctg ttgcgcgaag aggcgcgccg cggcgcggcc gtggccgtga ccctgcacga
      241 cctgccgctg gcctcgcgtc attgcgatga gatcgtggtt ctgcatcagg gccgtctggc
      301 ggcggacggc acgccggccg cggccctgtc ggacgaggct ttggcccagg tcttcggcat
      361 tgccgcgctg cgggttcccg atcccgaagg cgggcgggac gcgctggcgt cgttccggcg
      421 gatttagggt ttaggcgcca atccttcgac aagctccgga tgaggggcta aagagttgtc
      481 cctgccgggt ttgtcgaagc gcggaattct tcggagatca agatcatgcg agacggcgaa
      541 gacggcggcc tgggtgccca ttacagtcgc gccttgcgcg agttgcagat cgagctggtg
      601 aaattgcagc gtcacttgat cgaagagggc gcgcgcgtgc tcgtcatcct ggaaggtcgc
      661 gacggcgccg gcaaggacgg cacggtcaag cgtctgatcg agcacatgag cccgcgcgaa
      721 acgatcgtcc acgcgccggg caagccgtcc gaccgcgacc tcaccgaatg gtacttccag
      781 cgcttcgtgc ccaatctgcc ggcgggcggc gagttcgtcg tcttcaaccg ctcctggtac
      841 aatcgcgccg gcgtggaacg cgtcatgggc ttcagcacca agcagcaggt gaaggaattt
      901 ctccatgccg tcgtgccgtt cgaggacatg ctggtgcgcg acgggcttca cctgcgcaaa
      961 tattatctcg acatctccaa atccgagcag aagaagcggc tttcggaccg gcacaaagat
     1021 ccgctgaagc agtggaagag ctcgccggtc gacgatgcgg cgctcaaaaa ctggaaatcc
     1081 tacagcaagg cgcgcgacga gatgttcaag cgcaccagcc atccgcacgc gccctggaaa
     1141 gtggtgatcg ccaacgacaa gaagatcgcc cgcctggaat tcctgcgcga cctgctggcg
     1201 agcttcgact atccgggcaa ggacaagaag ctatcgcgcc ccgaccggag catcgtcttc
     1261 gactggtcgg agaagaaacg caagctgctc gcggattagc cgcctaaatc ctgtttcttc
     1321 tggagatact cggtgcggtc gatctggatc agcggctcgt ggcgaattac atttgaattt
     1381 gaaaaaggag acgcttatcg tattgacctt gaacaatatc attggggccg ggccggtgtc
     1441 cggccgggac ggacagttgg cggaatacaa agcaggcaag cgggaattcg gctcaagaac
     1501 gatctcgcca aggtcaagtc ggctgaagtt tcatcgacgg ttccgcgcca ccaatagaaa
     1561 tgcgtggtat taggtggcaa atgcatcttg aatttccgcc ccatctttgg gggtctgggg
     1621 gagataaact gatgcgtaat tcacgactgc atcgtttttg gcccgctgtt tttcgccggt
     1681 taggattggc gcgccggctc aattacgacc atcctctcac aatctccggc cgcgcgtttt
     1741 cgattccaat agccggcggg atcggagagg atctcctgaa gcttacaccg gatttcaaat
     1801 cggagatcat ccgccttttc ggcgacagga ttccgaattg ctttgtcgat gtcggcgcca
     1861 atctcggcca gaccgttatc gaggcattcg cgacccgaag atgggacagg tactttgccg
     1921 tggagcccaa tccaactgcc tgcgcctatt tggaaagcct tgtcaaagca aaccatcttc
     1981 cggttgacgt attgccgtgg ggcgccgggg cagacgcttc accgcatagc ttctgctttc
     2041 aaggaacgac ggatgcctct gccacgatgg caccggagga gcgccccggt gcctactcgc
     2101 cggaaatgtc gcgctcggtc gccacatatc cgctggatgt gctgctcgac atttttcctc
     2161 tgccgcccgg cttaatgatc aagatcgacg tcgaagggtt cgaggctgaa gttctcgccg
     2221 gagcgcgcaa catcttaagc gatgtgcggc ccgttattct atgcgaagtg cttcgcgcct
     2281 acgaaccagc gtcgcttacc ttcaccgaaa agaggatgag caagttagag gcaattctgg
     2341 cggccgttga ctatcggatt ttctacctcg aactggaaaa tgaagtctcc gggcgtattt
     2401 tgtcgatgaa ggaaattgcc tcgtttccga gagcactttg gagagaatct ccgtccggga
     2461 tggactacct atttgttcca gcagagatga cgttgcctga cttcttggct ggctgagggc
     2521 ttatggcgct tgcgccaaga taccgtggcg atggcgaccc cggggggact cgaacctccg
     2581 gcctacggtt taggaaaccg ccgctctatc ctgctgagct acggggtcgc gcgtcaggga
     2641 aactgccccc aagtcccgcg cttggcaagg gtcggtcgca cggcattgcg ctcttctctc
     2701 gcgccgcccg cgcggatacc gtatcatccg cccgacaagc gggatacgcc gcgaccgccg
     2761 aacaaggaga tgtcgaatca accatgaaac cgaactcgct ggccgtgctg gccgttttcc
     2821 tgcttgcgcc tttccccgcc aatgccgggg ctaacgacga cgcatttgcc gccatcggta
     2881 aatgcgcggc ggttacggaa gatgccgcgc gtttggcctg ctacgacaag attgccggcc
     2941 ggatgaaagc cgtcgaagcc gcgccgcaga cggcggcaca ggcacagccg caggccgccg
     3001 cgattccggc gcaaacggcc gccgccgctc cgcccgccgc caaagaggac caagaatact
//
LOCUS       DASWPU010000435         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_34704,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000435 DASWPU010000000
VERSION     DASWPU010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34704"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1706)
                     /locus_tag="VGC27_09370"
     CDS             complement(78..1706)
                     /locus_tag="VGC27_09370"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY0282818.1"
                     /translation="MARLIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYL
                     NVDPGTMSPHQHGEVYVTDDGAETDLDLGHYERFTGVSSGKADNITSGRIYQQVIEKE
                     RRGEYLGRTVQVIPHVTDMIKEFMLAGAEDVDFLLCEIGGTVGDIEGLPFFEAIRQLG
                     NELGREHHAFVHLTLVPYLDAAGELKTKPTQHSVKELRAIGIQPDILLCRSEKPLPDE
                     ERRKIGLFCNLPAQRVIPALDLSTIYEVPLAYHEVGFDNEILSVFGIKDAPRPDLSKW
                     QWVVDRVHNPEGEVRIAVVGKYMQVKDSYKSLSEALTHGGLANNVRVHLEMIDSEIFE
                     RSDAASFLENVNGILVPGGFGERGVEGKIRAVKFARDRKVPFLGICYGLHMAVIEVAR
                     DLAGLEGANTTENGRPPHPVIALMTEWTKGNRIETRAENSDLGGTMRLGAYPAVLTAG
                     SRVAEVYGTNEISERHRHRYEVNVNYIEQLAEHGLVVSGWSPDGKLPEIMEIPDHPWF
                     IGVQFHPELKSRPFAPHPLFRSFIAAAVKQGRLV"
     gene            complement(1819..2169)
                     /gene="secG"
                     /locus_tag="VGC27_09375"
     CDS             complement(1819..2169)
                     /gene="secG"
                     /locus_tag="VGC27_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY0282819.1"
                     /translation="MQTLLLVIELMIAIALVIVILMQRSEGGALGMGGGGSGLGGLFS
                     PRGASDTLTRTTAILAVLFFIVCLALNLLVAHTHVPRSILDSTATPARTAAPVQKPAA
                     PAPVQPVPAIPTPH"
     gene            complement(2291..3274)
                     /locus_tag="VGC27_09380"
     CDS             complement(2291..3274)
                     /locus_tag="VGC27_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282820.1"
                     /translation="MARLSFESAEALVYDPVSANRTATRAALYTLGFRNLETVATIED
                     LHEAIRNRPPDLALCEAQGADAELCQMIQMLRQGAAGYNPFIVIIVTAWEKTNTLVSR
                     VLNSGADDLILRPFSTKLMGERIEAHIERRKGFVITHDYVGPDRRRDMGRASPVELFE
                     PPNSLKMKAKERLSVEEATTRLDAELRTARDLFNSEKMRRDAFQICILWRLLQERMPG
                     EENYSEELSKLGRVTKAVTKRCRESEVEAAIEWCESVLAAVEGLELGVDRNASLHLLG
                     HAAMNLIQVLQPELSAADHLRKIDETVAMIRARRQAKEDAEGAELKMANKE"
BASE COUNT          614 a         1040 c         1055 g          645 t
ORIGIN      
        1 cggattgcta agaaagacac gctgtttacc ctgccccaac gcggggaggg aaaaaagaaa
       61 cttacgcggc atcaggatta caccagcctg ccctgcttca ccgccgcggc gatgaagctg
      121 cggaacagcg ggtggggagc aaacggccgc gatttcagtt caggatggaa ctgcacgccg
      181 atgaaccagg ggtgatcggg gatttccatg atctcgggca gcttgccgtc gggcgaccag
      241 ccggagacca ccaaaccgtg ctcggcgagc tgctcgatgt aattgacgtt gacctcgtag
      301 cggtggcgat gccgctcgga aatctcgttc gtcccataga cttccgccac gcggctgccc
      361 gccgtcagca ccgcgggata ggcgcccagc cgcatcgtgc cgcccaggtc gctgttttcc
      421 gcacgggttt cgatgcggtt gcccttggtc cattcggtca tcagggcgat gacgggatga
      481 ggcggcctgc cgttctcggt cgtgttggcg ccttcgaggc ccgccagatc gcgcgcgacc
      541 tcgatcaccg ccatgtgcag tccgtagcag atgcccagga acggcacctt gcgatcgcgg
      601 gcgaacttca ccgcccggat tttaccttcc acgccgcgct cgccgaaacc gccgggcacc
      661 aggatgccgt tgacgttttc aaggaagctc gccgcgtcgc tgcgctcgaa aatctcgctg
      721 tcgatcatct ccagatggac gcgcacattg ttggccagcc cgccatgggt gagcgcttcc
      781 gacagcgact tgtaggaatc cttcacctgc atgtacttgc cgacgacggc gatgcgtacc
      841 tcgccttcgg gattgtgcac ccggtccacg acccattgcc atttggagag gtccggccgg
      901 ggcgcgtcct tgatgccgaa gacgctgagg atttcgttgt cgaagccgac ctcgtgatag
      961 gccagcggca cctcgtagat cgtggaaagg tcgagcgcgg ggatcacgcg ctgggccggc
     1021 aggttgcaga acaggccgat cttgcggcgt tcctcgtccg gcagcggctt ttccgagcgg
     1081 cacaagagga tgtccggctg gatgccgatg gcgcgcagct ccttcaccga atgctgggtc
     1141 ggcttggtct tcagttctcc ggcggcgtcg agataaggca ccagggtcag atggacgaag
     1201 gcgtgatgct cgcgccccaa ctcgttgccg agctggcgga tggcctcgaa gaacggcaaa
     1261 ccctcgatgt cgccgaccgt gccgccgatc tcgcagagca ggaaatcgac atcctcggcg
     1321 cccgccagca tgaattcctt gatcatgtcg gtgacgtggg ggatcacctg cacggtgcgg
     1381 ccgagatatt cgccccggcg ctccttctcg atcacctgct gatagatgcg cccgctggtg
     1441 atgttgtcgg ccttgcccga cgacacgccg gtgaagcgct cgtaatggcc gaggtcgaga
     1501 tcggtctcgg cgccgtcgtc ggtgacgtac acctcgccgt gctggtgggg gctcatggtc
     1561 cccggatcga cgttgagata ggggtccagc ttgcgcagcc ggactttgta gccacgcgcc
     1621 tgcaaaaggg ctccaagagc cgcggaggca agaccttttc ccaaggaaga gaccacgccg
     1681 ccggtgatga agatcaaccg cgccatggaa ggccaccatg tcgaaagagg tgcgcgcact
     1741 caagcgtaaa gatacggttt tttgacagcc tgagattttg tctcagggcc gcctgacaag
     1801 ctgctgaaac ttatgagttt agtgcggtgt tggaatcgcc ggcaccggct gaactggcgc
     1861 tggtgccgca ggcttttgta ccggcgcggc cgtgcgggcc ggcgtagcgg tcgaatcaag
     1921 gatcgaccgc ggcacatggg tgtgtgccac cagcaggttg agggccaggc aaacaatgaa
     1981 gaacagcaca gccagaatgg ccgtggtgcg ggtcagcgtg tcggaggcgc cgcgggggct
     2041 gaacagcccg cccaggccgg agccgccgcc gcccatgccc agcgctccac cctccgaacg
     2101 ctgcatcaga atgacgatga cgagcgcaat cgcgatcatg agttcgataa ccagcaacag
     2161 ggtctgcatg gggcgcgcct tctttccggg cggcttctta gacgataggg gcgcgattgg
     2221 aaagaagcga atagcgaata gcgaatagcg aatagtaagc ccttgttccc tattccctat
     2281 tccctattcg ctactcctta ttcgccattt ttagctcggc cccctctgca tcctccttcg
     2341 cctgccgccg ggcgcggatc atggcgacgg tttcgtcgat cttgcgaagg tgatcggcgg
     2401 cggacaactc gggctggagc acctggatga gattcatggc cgcgtgaccg agcaggtgca
     2461 aggaggcgtt ccggtcgaca ccgagttcca gcccctccac cgcagcgagc acggattcac
     2521 accattcgat ggccgcctcg acctcgcttt cgcggcagcg tttggtgacg gccttggtca
     2581 cgcgccccag cttggacagc tcttcggaat agttctcctc gccgggcatt ctttcctgca
     2641 aaagccgcca caggatgcat atctggaacg cgtcgcggcg cattttctcg gaattgaaga
     2701 ggtcgcgcgc ggtgcgcagc tccgcgtcca gccgggtggt tgcctcctcg acgctcaacc
     2761 gctccttcgc cttcatcttg agcgagttgg gcggctcgaa cagctccacc ggcgacgctc
     2821 tgcccatatc gcgcctgcga tcgggaccga cataatcgtg ggtgatgacg aagcctttgc
     2881 gccgctcgat gtgggcttcg atgcgttcgc ccatcagctt cgtcgagaac gggcgcagga
     2941 tcagatcgtc ggcgccggaa ttcagcacgc ggctgaccag cgtgttggtc ttctcccaag
     3001 ccgtgacgat gatgacgatg aacggattgt agccggcagc gccttgccgc agcatctgga
     3061 tcatctggca cagttcggca tcggcgcctt gcgcctcgca caaggccaga tcgggcggac
     3121 gattgcggat tgcctcgtgc aaatcctcga tcgtggcgac cgtctcgagg ttgcggaagc
     3181 cgagcgtgta caatgcggcg cgcgtcgccg tccggttcgc ggaaaccggg tcatacacca
     3241 acgcctcggc cgactcgaat gagagcctcg ccatcaccac tccctacgct tcaagacttt
     3301 ggcaacaatt ttccacgccc gaagttaagg aaacgtgggc cgggttggtt aatc
//
LOCUS       DASWPU010000436         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_65423,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000436 DASWPU010000000
VERSION     DASWPU010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65423"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1307)
                     /locus_tag="VGC27_09385"
     CDS             complement(69..1307)
                     /locus_tag="VGC27_09385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0282821.1"
                     /translation="MPERMSPERSAHPVLFLILFLPMGITNGYVVVTLGYLLTHAGVS
                     VTAVAGLVSVSLLPQTWKVFWAPVIDTTLSNKSWYLYSGILLGLLMIATSAIPDPASH
                     LMLIGVLVFGFSLTSTFNMMAADSLMAHATSPAEKGRAGGWSQAGNLGGSGLGGGAGL
                     WLAQHVQGGWVVGAALGVTCILAALALLPLREPKAEHRVPSYLETLKNVGADVWSILR
                     ARMGFLAFVAMCLPIGAGAAQNLWAAVADDWHATADTVALVNGALGGVISMFGCVIGG
                     YLCDKLDRKTAFNLFSIALALSALAMAASPRSELMFILFTCFYAFILGFCYAAFGAVV
                     LEAIGKGAAATKYNLFAGIANAPIIYLTLIDGWAHQNGGTNGFLLAEGLVPIAGTCVF
                     ILVALATKRFFKARPAPAAT"
     gene            1523..>2996
                     /locus_tag="VGC27_09390"
     CDS             1523..>2996
                     /locus_tag="VGC27_09390"
                     /inference="COORDINATES: protein motif:HMM:NF009379.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0282822.1"
                     /translation="MSNTNGGRGPRTVALVGPYGSGKTTLLESILFTTGAAPRKGSVS
                     QNNTVGDTSPEARARQMSVEINCATTAYLDESFTFIDCPGSIEFLQDTLYAIQGVDAA
                     IVVIEPEPAKVQMLKPYLKRLADLKIPHLMFVNKIDKANGSLREILSTLQEASDTPLL
                     LRQIPIWENEVVTGFVDLALERAFAYREQSHSETIDIADREREKEARFAMLERLADYD
                     EHLMEELLSDVEPARDEIFGDLKREFAEGLVVPVLIGSAEGGHGITRLLKALRHEAPQ
                     VATTAARLGLAESGDTIVQVLKTVYSTHGGKLSLSRVLSGSLKDGAVLHTADGRDARV
                     GGIFALVGAAQTKLSEAKAGDTVALVRLEGIVTGDLLSNGKTAPPKKEVEMLPPVYRL
                     AIEAADRKDEVKLTAAIGKLIEEDPSLIFEQNAELKEMALAGQGEIHLKVAVEKLMSK
                     YGLKLNTHPASVPYKETIRKSVTQRGRHKRQSGGHGQFGDC"
BASE COUNT          620 a          969 c          959 g          448 t
ORIGIN      
        1 tttcgaagtt cgcagcatcc gctatatcag gctgcaagcg aacttcgaaa ccacgacact
       61 agaacgctct atgtcgccgc cggcgcgggg cgcgccttga aaaagcgctt cgtcgccagc
      121 gcgaccagga tgaagacgca agttccggcg atgggaacca ggccttcggc cagcagaaag
      181 ccgttggtgc ccccgttctg gtgcgcccaa ccgtcgatca gcgtcagata gatgatcggc
      241 gcgttggcga tgccggcaaa cagattgtat ttcgtcgccg ccgcgccctt gccgatcgct
      301 tccagcacca ccgcgccgaa ggcggcgtaa cagaagccca ggatgaaggc gtagaagcag
      361 gtgaatagga tgaacatcaa ctcgctgcgc ggcgaggccg ccatcgccag cgcgctcagc
      421 gcgagggcga tgctgaacag attgaacgcc gtcttgcggt ccagcttgtc gcagagatag
      481 ccgccgatca cgcagccgaa catcgagatg acgccgccga gcgcgccgtt gacgagcgcg
      541 accgtgtcgg cggtggcgtg ccagtcgtcc gcgaccgcgg cccacagatt ctgcgcggcg
      601 ccggcgccga tcggcagaca catcgcgacg aaagccagaa accccatgcg ggcgcgcagg
      661 atcgaccaga cgtccgcgcc cacgttcttg agagtttcaa gatagctcgg gacgcggtgc
      721 tccgctttcg gctcgcgcag cggaagtagc gccagcgcgg cgagaatgca ggtcacgccc
      781 agcgccgcgc cgacgaccca tccgccttgg acatgctgcg ccagccagag cccggcgccg
      841 ccgcccaatc ccgaaccgcc gagattgccc gcctgactcc agccgccggc gcggcctttt
      901 tccgccggag aggtcgcgtg cgccatcaga ctgtcggcgg ccatcatgtt gaaagtcgag
      961 gtcaggctga atccgaacac cagtacgccg atcagcatca aatgcgacgc gggatcggga
     1021 atggccgagg tggcgatcat cagcagaccg agaaggatgc cggaatagag ataccagctc
     1081 ttgttgctga gtgtggtgtc gatgaccggc gcccagaaca ccttccaggt ctggggcaga
     1141 aggcttaccg acaccagtcc cgcgacggcg gtcacgctga cgcctgcgtg cgtcagcaag
     1201 tagcccagag tcaccacgac atagccgtta gtgatgccca tcggcaaaaa aaggatcagg
     1261 aacagcacgg gatgggccga gcgttccggc gacatgcgtt caggcacggg ggaagaacct
     1321 tggattgaat cgcgctgcga cagtatagag cgcaaaaccg cgccgatagt ggggcgcggc
     1381 gcaacatcgc ccccttgcca acgtccccca acggcgcgag ggtttgtcgg cgaaaatagg
     1441 gcgtgtggcg cggcccgatc aacgggacga aatgtccgcc gccccccggg gatcccatcc
     1501 ccgcggcccg aaggaggcga agatgtccaa tacaaatgga ggaagagggc cacgcaccgt
     1561 ggccctggta ggaccctacg gcagcggcaa gaccacgctt ttggagagca ttctcttcac
     1621 caccggcgcc gcgccgcgca aagggtccgt ttcgcagaac aacaccgtgg gcgacaccag
     1681 cccggaggcg cgcgcccgcc agatgagcgt ggagatcaac tgcgccacga ccgcttatct
     1741 ggacgagagt ttcaccttca tcgattgccc cggttcgatc gaattcctgc aggatacgct
     1801 gtatgcgatc cagggcgtgg acgcggccat cgtcgtgatc gagccggaac ccgccaaggt
     1861 gcagatgctg aagccctatc tgaagcgtct cgccgacctc aagattcccc acctcatgtt
     1921 cgtcaacaag atcgacaagg cgaacggctc cctgcgcgag atcctctcca cgctgcaaga
     1981 agccagcgat acgccgctcc tgttgcgcca gatcccgatc tgggaaaacg aggtggtgac
     2041 ggggttcgtc gatctggcgc tcgagcgcgc tttcgcctac cgcgagcagt cgcactcgga
     2101 gaccatcgac atcgccgacc gggaacgcga gaaggaagcg cgcttcgcca tgctcgaacg
     2161 gctcgccgat tacgacgagc atctgatgga agaactgctg tccgacgtcg aaccggcgcg
     2221 cgacgagatt ttcggcgatc tcaaacggga attcgccgag ggcctggtgg ttccggtgct
     2281 gatcggctcg gccgaaggcg gccacggcat cacccggctg ctgaaggcgc tgcgccacga
     2341 agctccccaa gtcgccacca ccgccgcccg cctgggcttg gcggagagcg gcgatacgat
     2401 cgtgcaagtc ctcaagaccg tatattccac gcatggcggc aagctcagcc tgtcgcgcgt
     2461 gttgagcggt tcgctcaagg acggcgcggt gctgcacacc gccgacggcc gggatgcccg
     2521 cgtcggcggc atcttcgcgc tggtgggcgc cgcccagacc aagctttcgg aggccaaagc
     2581 cggcgatacg gtcgccctgg tgcgcctgga aggcatcgtg acgggcgatc tgctctcgaa
     2641 cggcaagacg gcgccgccga agaaggaggt ggagatgctt ccgccggtct atcggctggc
     2701 gatcgaagcc gccgaccgca aggacgaggt caagctgacc gccgccatcg gcaagctgat
     2761 cgaggaagac ccttctttga tcttcgaaca gaacgccgag ctgaaggaaa tggcgctggc
     2821 gggccagggc gaaatccacc tgaaggtcgc ggtcgagaag ctgatgagca aatacgggct
     2881 caagctcaac acccatccgg ccagcgttcc ctacaaggaa accatccgca aatcggtgac
     2941 ccagcgcggc cgccacaagc ggcagtccgg cggtcacggc cagttcggcg actgcg
//
LOCUS       DASWPU010000437         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_120302,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000437 DASWPU010000000
VERSION     DASWPU010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120302"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /locus_tag="VGC27_09395"
     CDS             <1..1628
                     /locus_tag="VGC27_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEY0282823.1"
                     /translation="MLALASLAHRLKGLSLRTKRTIGTFAVLGLALFIGDGMLTPAIT
                     VLSAVEGLSVGRETFQPLILTLVLVILVGLFTIQSRGTAKVGKLFGLVMVVWFLVLGI
                     LGLFSILHAPQILWALSPHYGLMLFVREPWTAFVALGSIVLAVTGCETLYADMGHFGK
                     FPIRMAWLGFAFPALVLNYFGQGAALLIAPNQASAAFYAVAPPWAHYPLVALATVASV
                     VASQAVISGVYSITRQAVQLGQLPRMEIRHTSATDYGQIYVPRMNAYLCVGVVLIVLI
                     FKSSNALAAAYGIAVTGEMFLSTLLVGLLAVKLWHWKKWFVIPLFGALVLIDLAFLSS
                     NALKIAEGGWLPLFIAACVYIVMETWRQGRRTHLEHIRNDSMPMDLFLERAEKTPLRV
                     AGTAVFLSSRTDVVPGALLHNLKHNKVLHERVIVCQVMVDDTPFVPPEKRVEVEKLGK
                     GFFAVRIHHGFFEAADVPRALAEARPYGLVVDPETATFFIGRETLVPAETPVLGRWRT
                     WLYMQLASNALSPARFYHLTPNRVVELGTQITI"
     gene            complement(1695..>2186)
                     /locus_tag="VGC27_09400"
     CDS             complement(1695..>2186)
                     /locus_tag="VGC27_09400"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0282824.1"
                     /translation="TGGFALAAARAGAKEVAGLDSSAPALALAEDAAASGGLKANFVK
                     TDVFEELERLWAGREKFDLVVADPPPFVKAKKDLEAGAKAYRKLARLSACVTAPGGFL
                     LLASCSHNISTERFAQECAAGVTRAGRAARLIRQAGAACDHPIHPLLPESAYLKALVY
                     ALD"
BASE COUNT          345 a          725 c          686 g          430 t
ORIGIN      
        1 gcatgctggc cctggcctcg ctggcgcatc gcctgaaggg actgagcctg cgcaccaagc
       61 ggacgatcgg caccttcgcg gtgctgggac tggccctgtt catcggcgac ggcatgctga
      121 cgccggcgat cacggtgctc agcgcggtgg aaggcttgag cgtggggcga gaaaccttcc
      181 agccgctgat cctgactctg gtgctcgtca tcctggtcgg ccttttcacg atccagtcgc
      241 gcggcaccgc caaggtcggg aaattgttcg ggctggtgat ggtggtctgg ttcctggtgc
      301 tcggaatact tggactgttc tcgatcctcc atgcgcctca aatcctgtgg gcgctgagcc
      361 ctcattacgg cctgatgctg ttcgtgcgcg aaccctggac cgcttttgtg gcgctcggct
      421 cgatcgtgct ggccgtcacc ggatgcgaaa cgctttatgc cgacatggga catttcggaa
      481 aattcccgat ccgcatggct tggctcggct tcgccttccc ggcgctggtg ctgaactatt
      541 tcgggcaagg cgcggcgctt ctgatcgctc ccaaccaagc ctccgccgcg ttctatgcgg
      601 tggcgccgcc ctgggcccat tacccgctgg tggcgctggc caccgtcgcc tccgtcgtcg
      661 cctcgcaggc ggtgatctcg ggggtctatt cgatcacccg ccaggcggtg caactgggcc
      721 agttgccgcg tatggaaatc cgccacacct cggcgacgga ttacggccag atctacgtgc
      781 ccaggatgaa cgcctatcta tgcgtcgggg tggtcctgat cgtgctgatt ttcaaaagct
      841 ccaacgcgct ggcggcggcc tacggcattg ccgtcaccgg cgaaatgttc ctcagcacgc
      901 tcctggtcgg tttgctcgcg gtcaagctct ggcactggaa gaagtggttc gtcattcccc
      961 tcttcggcgc tttggtgctg atcgatctcg cgttcctctc ctccaacgcg ctcaagatcg
     1021 ccgaaggcgg ctggctgccg ctgttcatcg cggcttgcgt ttacatcgtc atggaaacct
     1081 ggcggcaggg ccggcgcacg catctggagc atatccgcaa cgactcgatg ccgatggacc
     1141 tgttcctgga gcgtgccgaa aaaacgccct tgcgcgtggc gggcaccgcg gtgttcctgt
     1201 cctcgcgcac cgacgtggtt cccggcgcgc tgctgcacaa tctcaagcac aacaaggtcc
     1261 tgcacgagcg cgtgatcgtc tgccaggtga tggtggacga cacgcccttc gtgccgcccg
     1321 aaaagcgcgt cgaggtcgag aaactcggca agggattctt cgccgtgcgc atccatcacg
     1381 gcttcttcga ggcggcggac gtgccgcgcg ccctggccga ggcgcggccc tatggcctgg
     1441 tggtcgatcc cgaaactgcc accttcttca tcggccgcga aaccctggta ccggcggaga
     1501 cgcccgtgct cggacgctgg cgcacctggc tttacatgca gctggcgtcc aacgccctgt
     1561 cgccggcgcg cttctatcac ctgacgccca accgcgtggt ggaactgggc acgcagatta
     1621 cgatctgaaa agcgaatagg gagtagcgaa tagcgaggtg ggaactcctc tattcgccat
     1681 tcgctactcg ccattcagtc cagcgcgtag accagcgctt tcagataggc gctttccggc
     1741 agcagcggat gaatcggatg atcgcaggcc gcgcccgctt ggcggatcag ccgcgccgcc
     1801 cggccggcgc gagtgacgcc cgcggcgcat tcctgcgcga aacgttcggt cgaaatgttg
     1861 tgcgaacaag aggccagcag caggaaccct cccggcgccg tgacgcaggc gctcagccgc
     1921 gccagtttgc gataagcctt ggcgcccgct tcgagatcct tcttggcctt cacgaaaggc
     1981 ggcggatcgg cgaccaccag gtcgaatttc tcgcgccccg cccacagccg ttccagctct
     2041 tcaaagacat cggtcttgac gaaattggcc ttgaggccgc cgctggcggc ggcgtcctcg
     2101 gccagcgcca aagccggggc ggaactgtcg agccccgcca cttctttcgc accggcgcgc
     2161 gcggcggcca gcgcgaatcc gccggt
//
LOCUS       DASWPU010000438         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_9331, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000438 DASWPU010000000
VERSION     DASWPU010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9331"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VGC27_09405"
     CDS             <1..839
                     /locus_tag="VGC27_09405"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEY0282825.1"
                     /translation="ELILLLADDRIEVARPIILRSPLLTEADVLRMVVECDVGHQEAV
                     AARENISEPVTEALAKCEAESVLIALVRNQTAKISSTTYETLVEKSRRVAGLQDPLVR
                     RNELPAELAVRMCEWVSDALKTYISHNYNVEAGRLDAAFEHAETIVKSEPAAPKTPPA
                     EGAQKLIDKLSNAGQLKAGFLLRVLHQGQIDLFDLALARMLDLPLPELRQILYEKGPK
                     PVALACRAVGIDRCVFATVFNLSRQARKMRPVLSASDMSEVETVFNSFSKPGAMMELH
                     RH"
     gene            1181..2170
                     /gene="moaA"
                     /locus_tag="VGC27_09410"
     CDS             1181..2170
                     /gene="moaA"
                     /locus_tag="VGC27_09410"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY0282826.1"
                     /translation="MIDPFGRHITYLRVSLTDRCDLRCVYCMAAQPEFLPRAELLSLE
                     EIERVCAVFMRKGIERIRITGGEPLVRRGALGLIERLGAWTGGGGLRELTLSTNGGQL
                     VRFARPLAAAGVKRINVSLDTRDPDKFRAITRWGEIAKVLAGIDAALDAGLDVKINTV
                     ALKGVNEDEFAGLIRWTHGRGMDLTLIETMPMGGIDADRVAQYLPLSMVRASLARHFT
                     LEESCYRTGGPARYVRVAETGGRLGFITPLTHYFCDSCNRVRLTCTGTLFMCLGQEDA
                     ADLRAPLRASSDDGMLDEAIDAAIARKPKGHDFMIDRRHSAPAVARAMSMTGG"
     gene            2173..2934
                     /locus_tag="VGC27_09415"
     CDS             2173..2934
                     /locus_tag="VGC27_09415"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003406.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEY0282827.1"
                     /translation="MAFKLHFVATPVPEGQAALANLRQQYDDVGPDKADVIVALGGDG
                     FMLQTLHAFIQRSKPIYGMNFGTVGFLMNEYNEADLTKRLAKAEQATVHPLRMRATTA
                     KECIDALAFNEVSLLRQTRQATKLRILVDGKVRMPELICDGALVATPAGSTAYNFSAH
                     GPILPIDSALLALTPISAFRPRRWHGALLPHRARVRFEILETQKRPVSAVADGLEVRD
                     VVSVDVAQDDKISMVMLFDKGHSLDERILTEQFTD"
     gene            3021..>3301
                     /locus_tag="VGC27_09420"
     CDS             3021..>3301
                     /locus_tag="VGC27_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0282828.1"
                     /translation="MSGYRFDRLKILVVDDNQHMRKLVGTILQAFGTNQIYEASDGER
                     AWIVLHDVNPDVILLDWQMEGMNGIDFARMVRTSPNSPNPFVPIIMLTG"
BASE COUNT          619 a         1087 c         1014 g          581 t
ORIGIN      
        1 atgaactcat cctgctcctg gccgacgaca gaatcgaagt cgccaggccg atcatcctgc
       61 gcagcccgct gctgaccgaa gccgatgtgt tgcggatggt cgtggaatgc gacgtcggtc
      121 atcaggaagc cgtggcggcg cgcgagaata tcagcgaacc ggtcaccgag gcgctggcga
      181 aatgcgaagc ggagagcgtt ctcatcgcgc tcgtccgcaa ccagaccgcc aagatatctt
      241 ccacaaccta cgaaacgctg gtcgagaaat cccggcgcgt agccggcctg caggacccgc
      301 tggtccgccg caacgaactt cccgccgaac ttgccgtccg catgtgcgaa tgggtgtccg
      361 acgcactcaa gacctacatc agccataact acaatgtgga agccgggcgg ctggacgccg
      421 ccttcgagca cgccgaaacg atcgtgaaaa gcgagccggc cgcacccaag actcctccgg
      481 cggaaggcgc acaaaagctc atcgacaagc tgtccaacgc cggacagctc aaagccggct
      541 tcctgctcag ggttttgcac caggggcaaa tcgatctgtt cgatctggcg ctcgcccgca
      601 tgctcgacct gccgctcccc gaactgcggc agatcctata cgaaaaaggc cccaaaccgg
      661 tggcgcttgc ctgtcgcgcc gtcggcatcg accgttgcgt cttcgccacc gtgttcaatc
      721 tctcgcggca ggcccgcaag atgcggccgg ttctgtcggc ctccgacatg agcgaagtgg
      781 agacggtgtt caacagcttc agcaagcctg gcgcgatgat ggaactccac aggcattaag
      841 aggtcttagt attcgtccgg gcggcgagcc ctgcgagggc gcgcggacat atttttcgcg
      901 agccgcgttt cgtgcgaaaa tcgctcggat ggcaggagag gcgcgaagaa cgtatcgggt
      961 aatacggtcg agcgcttcga caaaccagcc gacgattttc gccgaaaccc ttcgggacgc
     1021 gccgatttta cccgcccggt tcgtcgccgc gcttgcccga tgttcccgca tcgacctgcg
     1081 cgccgtcgct ggctgtcgcc agctccacct gccgggtcgg agcaaaatcg gcagcgtcgc
     1141 ggccgtgaca agtatgttcg cgcgccctag gcttgacggt atgatcgacc cgttcggccg
     1201 ccacatcacc tatctgcgcg tctcgctgac cgaccgttgc gaccttcgtt gcgtctattg
     1261 catggcggcg cagcccgagt tcctgcccag ggcggagctt ctgagcttgg aagagatcga
     1321 gcgcgtctgc gccgttttca tgcgcaaggg catcgagcgt atccgcatca cgggcggcga
     1381 accgctggtc cggcgcggcg cgcttggcct gatcgagcgg ctgggcgcct ggaccggcgg
     1441 cggcggcctt agagaactga ccctcagcac caacggcggc cagcttgtcc gcttcgccag
     1501 accgctggcc gcggcgggcg tcaagcggat aaacgtctcg ctcgacacgc gcgaccctga
     1561 caaattccgc gccatcaccc gctggggcga aatcgccaag gtgctcgccg gcattgacgc
     1621 ggcgttggac gcgggcctcg acgtcaagat caacacggtg gcgctgaaag gcgtcaacga
     1681 ggacgaattc gccggcctga tccgctggac gcacgggcgc ggcatggacc tcaccctgat
     1741 cgaaaccatg ccgatgggcg gcatcgacgc cgaccgcgtc gcgcaatacc tgccgctgtc
     1801 catggtgcgc gccagcctgg cgcggcactt cacccttgag gagtcctgct accgcaccgg
     1861 cgggccggcg cgttatgttc gggtggccga aaccggcggc cggcttggct tcatcacccc
     1921 tctgacccat tatttctgcg acagttgcaa ccgcgtccgg ctgacttgca cgggaacctt
     1981 gttcatgtgc ctgggccaag aggatgcggc cgatttgcgt gcccccttgc gcgcgtcgag
     2041 cgacgacggg atgctggacg aagccataga cgccgccatt gcacgcaagc cgaagggcca
     2101 cgatttcatg atcgaccgtc ggcattccgc tccagcggtg gcacgtgcga tgtcgatgac
     2161 cggaggctga ccttggcgtt caagctgcat ttcgtcgcca cccccgttcc ggaaggacaa
     2221 gccgcgctgg caaacctgcg ccagcaatat gatgatgtcg gccccgacaa ggcggatgtc
     2281 atcgtggcgc ttggcggcga cggcttcatg ctccagacgc ttcatgcctt catccagcga
     2341 agcaaaccga tctacggaat gaatttcggc accgtcggct tcttgatgaa cgaatacaac
     2401 gaagccgatt tgacgaagcg gctggccaag gccgaacagg cgacggttca tccgctcagg
     2461 atgcgcgcca cgacggcgaa ggaatgcatc gacgcgctcg ccttcaacga agtctcgctg
     2521 ctgcggcaga cgcgccaggc caccaagctg cgcatcctgg tcgacggcaa agtgcgcatg
     2581 cccgaactga tctgcgacgg cgccctggtg gccacccctg ccggcagcac cgcctacaat
     2641 ttctcggccc acggccccat tctgccgatc gactccgccc tcctggcgct gaccccgatc
     2701 agcgccttca ggccgcggcg ctggcacggg gcgctgctgc cgcaccgcgc gcgggtgcgc
     2761 ttcgagatcc tggagacgca gaagcgcccg gtcagcgccg tggccgacgg cctggaagtc
     2821 cgcgacgtgg tctccgtcga cgtggcgcag gacgacaaga tttccatggt catgctgttc
     2881 gacaaggggc acagcctcga tgaacgaatc cttaccgagc aatttaccga ttaggttgtc
     2941 ccgcttcacc cgtcgttaag cgccgttaag taacctgttc cttgcgggcg cgcatgggcg
     3001 tcgggcgtgt tggacggatc atgagcggct atcgcttcga caggctgaaa atactcgtcg
     3061 tcgacgacaa ccagcacatg cgcaagctgg tcggcacgat attgcaggcg ttcggcacga
     3121 atcagattta cgaggcatcc gacggcgagc gcgcctggat cgtcctgcac gacgtcaatc
     3181 ccgatgtcat tctgctcgac tggcagatgg agggcatgaa cgggatcgat ttcgcccgca
     3241 tggtgagaac ctctccgaat tcgcccaatc ccttcgtgcc gatcatcatg ctgaccggcc
     3301 a
//
LOCUS       DASWPU010000439         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_89251,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000439 DASWPU010000000
VERSION     DASWPU010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89251"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VGC27_09425"
     CDS             <1..654
                     /locus_tag="VGC27_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HEY0282829.1"
                     /translation="GRIAAGGIDYLKDDHGLADQSYSPFAERIPACAEAVADAAARTG
                     TPTRYLPSLNGNLDSLRKQIALCRSCGLDTVLVAPLIAGLAQFHTLVKENPDIAFAAH
                     PTMAGARIMPSFLLGKLFRLLGADATIFPNYGGRFGYTPEECKRLAGFALQDWPGIKP
                     CVPVPAGGMSLARIPEILDFYGTDVMLLIGGSLLSTRERITEETARYVEAARAYRAQ"
     gene            657..1109
                     /locus_tag="VGC27_09430"
     CDS             657..1109
                     /locus_tag="VGC27_09430"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0282830.1"
                     /translation="MDEKNPIRRFNAFRWEETDLLAYKEEGSAPFKAITRQTLFRDPS
                     LKCELRYFEMAPGGYSTLERHEHMHAVLILRGHGEVLVGDKVSNIDTNDLVTIEPWTW
                     HQFRAGPDAPLGFLCMVNAERDKPSLPTADDRKLLEANPKISAFLKGE"
     gene            complement(1264..2112)
                     /locus_tag="VGC27_09435"
     CDS             complement(1264..2112)
                     /locus_tag="VGC27_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0282831.1"
                     /translation="MTNGPPRIFDAHAQALHRDRAARMTGERFLLHEAADGIAARLDP
                     VNRTFGAALAVDGRAAALLQGIAPTWRIGLFDAGEQLDSGDGGFDLAVSILSLHGIND
                     LPGALVQIRRALKPDGLFVAALFGGATLCELRDAFTSGEIETIGGITPRVTPFADVRE
                     LGALLQRAGFALPVADVERTCVLYRDFATLANDLRLMGETNALIKRQGLSRATLRASL
                     AYYAAKHSDADGRLRATFDIVYLTGWSPHESQQKPLKPGNAKVRLAQALGTTEHSAGD
                     KAKPRR"
     gene            2136..>2836
                     /locus_tag="VGC27_09440"
     CDS             2136..>2836
                     /locus_tag="VGC27_09440"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY0282832.1"
                     /translation="MVIDNGWKGVGRQLLDLVYPPLCLACREHVSEPDSLCPECWRTL
                     HFLDGPVCAACGLPFEIDPGGEMLCAGCLAHSPAFDKARAVLRYDEASRKPILALKNM
                     DRLDLAPAFGRWLERAGRELLTQSDLIVPVPLHRFRLWKRRYNQSAELARALSRLGGL
                     PVDPFALCRVRSTPNQGKMPSASARRRNVRGAFAVPEKRRSAVNGRQILLIDDVLTTG
                     ATANACAKALKKAGAR"
BASE COUNT          478 a          969 c          879 g          510 t
ORIGIN      
        1 ggccgtatcg ccgccggcgg catcgactac ctcaaggacg atcacggtct ggccgaccag
       61 tcctacagtc cgttcgccga gcgcatcccg gcctgcgccg aagcggtggc ggacgccgcc
      121 gcgcgcaccg gcacgccgac gcgctatctg cccagcctca acggcaatct ggattctctg
      181 cgcaagcaga ttgcattatg ccgttcctgc ggcctcgaca cggtgctggt cgcgcctttg
      241 atcgccggac tggctcaatt ccacacgctg gtgaaggaga atcccgatat cgccttcgcc
      301 gcccatccca cgatggcggg cgcccggatc atgccgtcgt tcctgctggg caagctgttc
      361 cgcctgctcg gcgcggacgc gacgattttc cccaattacg gcggccgttt cggctacacg
      421 ccggaagagt gcaagcgcct tgccggtttc gcgctccagg attggcccgg catcaagccg
      481 tgcgtgccgg ttccggcggg cggcatgagc cttgcgcgca ttccggaaat cctggatttc
      541 tacggtacgg atgtcatgct gctgatcggc ggcagcttgc tgtcgacgcg cgagcggatc
      601 accgaggaaa ccgcgcgcta tgtcgaagcg gcgcgcgcct accgggcaca gtgaccatgg
      661 acgagaagaa ccccatccgc cgtttcaacg ccttccgctg ggaggaaacg gatttgctcg
      721 cctacaagga ggagggcagc gcgccgttca aagcgatcac gcggcaaact ctgttccgcg
      781 atccgagcct caaatgtgaa ttgcgctatt tcgaaatggc gcccggcggc tattcgaccc
      841 ttgaacgcca tgagcatatg catgccgtgt tgatcctgcg cgggcacggc gaagtcctcg
      901 ttggcgataa agtcagcaac atcgacacca acgacctcgt caccatcgag ccttggacct
      961 ggcatcaatt ccgcgccggc cccgacgcgc cgcttggctt cctctgcatg gtcaatgccg
     1021 agcgcgacaa gccttcgctg ccgactgccg acgaccgcaa actgctcgaa gccaatccca
     1081 aaatctcggc gttcctgaag ggtgagtgac gacagtccgg aatgaattgc atctttaata
     1141 tcgtcatggc cgggcttgcc gccttcgcta aagctacggc ggccgtgtga atctgctctc
     1201 gcccgccgaa gtcccggcgt aggcgggacc cggccatcca gtggatatat atccaaattg
     1261 gcgctagcgc cgcggctttg ccttatcgcc cgcagaatgt tcggtcgtac ccagcgcttg
     1321 cgccagccgc actttcgcgt tgccgggttt caacggtttc tgctggcttt cgtgcggcga
     1381 ccagccggtc agataaacga tgtcgaacgt cgcgcgcagc cgcccgtcgg catcgctgtg
     1441 cttggccgca taatacgcca gcgacgcgcg caacgtggcg cgcgacaacc cttgccgctt
     1501 gattagcgcg ttggtttcgc ccatcagccg caagtcgttt gccagggtgg cgaaatcgcg
     1561 atagagcacg caggtgcgtt ccacgtccgc cacgggcagg gcgaagcccg cccgttgcaa
     1621 cagcgcgccc agttcgcgca cgtcggcgaa cggcgtcacg cgcggcgtga tcccgccgat
     1681 ggtctcgatc tcaccggagg tgaaggcatc gcgcagttcg cacagcgtcg cgccgccgaa
     1741 cagcgctgcc acgaataatc cgtccggctt cagggcgcgg cggatctgaa ccagcgcgcc
     1801 aggcaggtcg tttatcccat gcagcgacag gatgctgacg gccagatcaa accccccatc
     1861 gccgctgtcc agctgctcgc cggcgtcgaa gaggccgatg cgccaggtcg gcgcgatgcc
     1921 ttgcagcagc gccgccgcgc gcccatctac ggcgagcgcc gccccaaacg tccggttcac
     1981 ggggtcgagc cttgccgcta tgccgtccgc ggcttcgtgc agcagaaagc gctcgcccgt
     2041 catccgcgcc gcgcggtcgc gatgcagcgc ctgtgcatga gcgtcgaaaa tccgaggcgg
     2101 tccgttcgtc atcgcgctac actagcaaaa tggggatggt aatcgacaac ggttggaagg
     2161 gcgtggggcg ccagctcctc gatctcgtct atccgccgct ctgcctcgcc tgccgcgagc
     2221 acgtctcgga gccggacagc ctgtgccccg aatgctggcg cacgcttcat ttcctcgacg
     2281 gtccggtttg cgccgcctgc gggctgccgt tcgaaatcga tccgggcgga gaaatgctgt
     2341 gcgcgggttg ccttgctcat tcgcctgcct tcgacaaggc ccgcgcggtc ctgcgctatg
     2401 acgaggccag ccgcaaaccg atcctggccc tcaagaacat ggatcggctt gacctagccc
     2461 ctgcctttgg gcgctggctg gagcgggcag gacgtgaact gctgacccag tccgatctga
     2521 tcgtgccggt gccgctgcat cgcttccggc tatggaagcg gcgctacaac cagtccgctg
     2581 agctggcccg ggcgctgtcg cggctgggcg gtttgccggt cgatccgttc gcgctttgcc
     2641 gcgtccgttc gacgcccaac cagggcaaga tgccgtcggc cagcgcccgc cgccgcaacg
     2701 tccgcggcgc ctttgccgta cccgaaaagc gccgctccgc cgtgaacggc aggcaaatcc
     2761 tgctgatcga cgatgttctg accaccggcg cgacggccaa tgcctgtgcc aaagccttga
     2821 aaaaggccgg ggcgcg
//
LOCUS       DASWPU010000440         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_46802,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000440 DASWPU010000000
VERSION     DASWPU010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46802"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2118
                     /gene="alaS"
                     /locus_tag="VGC27_09445"
     CDS             <1..>2118
                     /gene="alaS"
                     /locus_tag="VGC27_09445"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY0282833.1"
                     /translation="FSFGDYFKEDAIAFAWDFVTKTAALPKDKLLVTVHPSDEQARLL
                     WKKIAGLADARIIGHKDNLWAMGPIGPCGYCSEIFFDQGDAVAGGPPGSPDEDGDRFL
                     EFWNLVFMQFEQVDENTRNDLPKPSIDTGLGLERLAAIQQGVHNNYEIDLFHRLIEVA
                     EEASGAKAAGDALASHRVIADHLRAASFLIADGVLPSNEGRGYVLRRIMRRGMRHAHL
                     IGSKDPLMHRLVPALVAEMGAAYPELTRAEALIAETLKLEETRFRETLARGLKLLDET
                     TGALKSGDRLAGDVAFKLYDTFGFPLDLTQDALKSRGIAVDTAGFDAAMAKQKADARK
                     AWAGSGESATDAQWFAILDEVGKTEFLGYDTEEAEGTVVALVKDGERVLKAKAGETVH
                     LLTNQTPFYAESGGQVGDTGALKSARAEGKVIDTEKKLGALYVHVVELTKGEILPGDS
                     VDLAVDGERRRATRANHSATHLLHAALKRVLGSHVAQKGSLVAPDRLRFDFSHPKPVT
                     EDELERVEALVNSVVRQNTDVVTRLMPTDKAIEAGAEALFGEKYGDEVRVLSMGEDDE
                     RVYSVELCGGTHVRRLGDIALFTILSESAVAAGVRRIEALTSEGARRYLSGQAEIARE
                     AAAALKTSTGELAARVTQLADERRRLEREIADARKALALAGPARESADETKIIAGVKA
                     VLRVVEGVLAKDLKGLADDAKTKI"
BASE COUNT          396 a          703 c          694 g          325 t
ORIGIN      
        1 ttctccttcg gcgactattt caaggaagac gccatcgcct tcgcctggga tttcgtcacc
       61 aagacggcgg cgctgcccaa ggacaagctg ctggtgacgg tccatccttc cgacgagcaa
      121 gcacgcctgt tgtggaagaa aatcgccggg ctcgcagacg cgcgcatcat cggccacaag
      181 gacaatttgt gggcgatggg ccccatcggc ccttgcggct actgctcgga aatcttcttc
      241 gaccagggcg atgcggtggc cggcgggccg cccggctcgc ctgacgaaga cggcgaccgc
      301 ttcctcgaat tctggaacct cgtcttcatg cagttcgagc aggtggacga gaacacccgc
      361 aacgatctgc ccaagccctc catcgatacc ggcctggggc tggagcgcct cgccgccatc
      421 cagcagggcg tgcacaacaa ttacgagatc gacctgttcc accgtctgat cgaagtggcg
      481 gaagaagcct cgggcgccaa ggcggcgggc gacgcgctcg ccagccatcg cgtgatcgcc
      541 gatcatctgc gcgcggcatc gttcctcatc gccgacggcg tgctgccctc gaacgaaggg
      601 cgcggctacg tgcttcgccg catcatgcgc cgcggcatgc gccatgccca tctgatcggc
      661 tcgaaggatc cgctgatgca ccgcctggtg ccggccctgg tcgccgagat gggggcggcc
      721 tatccggaac tgacccgcgc cgaagcgctg atcgccgaaa ccctgaagct ggaggaaacc
      781 cgcttccgcg aaactctcgc gcgaggactg aaactgctcg acgagaccac aggcgcgctc
      841 aaatccggcg accggctggc gggcgacgtt gccttcaagc tttacgacac cttcggtttt
      901 cccctcgatc tcacccagga cgcgctgaaa tcgcgcggca tcgcggtgga taccgccggt
      961 ttcgacgcgg cgatggccaa gcagaaggcc gacgcgcgca aggcctgggc cgggtccggg
     1021 gaatccgcga ccgacgcgca atggttcgcc attctcgacg aggtcggcaa gacggagttt
     1081 ttaggctacg acaccgaaga agccgaaggc acggtggtgg cgctggtcaa ggacggcgag
     1141 cgtgtgctca aggccaaggc gggcgagacc gtccatctac tgaccaacca gacgccgttc
     1201 tacgccgaat ccggcggcca ggtcggcgac accggcgcgc ttaaatccgc ccgcgccgaa
     1261 ggcaaggtga tcgacaccga aaagaagctc ggcgcgctgt acgttcacgt cgttgaactg
     1321 accaagggcg agatcttgcc gggcgactcg gtcgatcttg cggtggacgg cgaacggcgc
     1381 cgcgccaccc gcgccaacca ttcggcgacc catcttctgc atgccgcgct caagcgcgtg
     1441 ctgggaagcc atgtcgcgca gaaaggttcg ctggtggcac ccgaccggct gcgcttcgat
     1501 ttcagccatc ccaagccggt gacggaagac gagcttgagc gcgtcgaagc tctggtgaac
     1561 tccgtcgtgc gccagaatac cgacgtcgtc acccgcctga tgccgaccga caaggcgatc
     1621 gaggcgggcg cggaagccct gttcggcgag aaatacggcg acgaggtgcg cgtgctttcg
     1681 atgggcgagg acgacgagcg ggtctattcc gtcgaactgt gcggcggcac ccatgtgcgc
     1741 cggctgggcg atatcgccct gttcacgatc ctgtccgaaa gcgcggttgc cgcgggcgtg
     1801 cgccgcatcg aggcgctgac atccgaaggc gcgcgccgct atctttccgg ccaggccgag
     1861 atcgcgcgcg aagcggccgc ggcgctcaag acgagcactg gcgaactcgc cgcgcgcgtg
     1921 acccagcttg ccgatgagcg ccgcaggctc gaacgcgaga tcgccgacgc gcgcaaggcg
     1981 ctggcgctcg ccggccccgc gcgggaatcc gccgacgaga cgaaaatcat cgcaggcgtg
     2041 aaggcggtgc tgcgcgtggt cgaaggcgtc ttagccaagg acctcaaagg cctcgccgac
     2101 gatgccaaga ccaaaatc
//
LOCUS       DASWPU010000441         2986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_41122,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000441 DASWPU010000000
VERSION     DASWPU010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41122"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VGC27_09450"
     CDS             complement(<1..1185)
                     /locus_tag="VGC27_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0282834.1"
                     /translation="MSAEPEVCKDGDRGIFAGRIDILYALGRHYLSLPFAALAVSATL
                     FAGVPLTILPFLPLVLLIVVAIVAEQLTQTYKHRAPGTSPQYWARRYTFISAIAGASW
                     GVGVFFWFVPDSFAAQAYLCLAFMGMTATEFIARSAHRRAYLAHAFFSLGPLIAMLLI
                     EGSLYSDMTAVLVFCFAGVLAGYCRGMARLLDESIRLRLENKDLIEKMSTEKGDAEAA
                     RDDAEAGAKAKSIFIANISHELRTPLNALLGMAQLLDRAELGQPHGDHVKVMLESGRG
                     LQTLLEDVIALTQDDSDRLSDKECDPVQAARAVTRLCQPLAWQKGLRLTVTAGASVPR
                     AAADPRRVRQALLKLTDNALKFTDSGGVEIRVETFAKDGADFVRFAVIDTGLGVPEEI
                     EPR"
     gene            1247..2842
                     /locus_tag="VGC27_09455"
     CDS             1247..2842
                     /locus_tag="VGC27_09455"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001968.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY0282835.1"
                     /translation="MTSSPSLHAEALAARAWPFEEARKLLARMEKRGSGREVIFETGY
                     GPSGLPHIGTFGEVARTTWVRRAFSLMSGAKTRLLAFSDDMDGLRKVPANLPNQEMLA
                     AHLGKPLTAIPDPFGTHDSFGAHNNARLKAFLDRFGFDYEFLSSTDCYKSGRFDEALL
                     TVLAHYEKVKDVVLPTLGPERRATYSPFLPISPKTGIVLQVPILEWDAKRGTIVFEED
                     GKKQELPVTGGNVKLQWKADWAMRWLALGVDYEMAGKDLISSVELSSKIVRAIGGAPP
                     EGFNYELFLDDQGQKISKSKGNGLSVDEWLTYGTEESLALFMFQKPRAAKKLYFDSIP
                     KTVDDYIALLDAYRAKEKSAAERLESPIWHIHAGTPPDERYPVSFALLLNLVSASNAH
                     NREVLWGFIQSYAPQANPEANPGLDKLVGYAIKYYDDFVKPQKRYRAPSDKERAALAD
                     LADKLRAFGAERDAEKVQFEVYEVGKRHDFEPLRDWFKALYEVLFGQSAGPRFGSFAA
                     LFGCAETAALIERALQGEFVSAA"
BASE COUNT          552 a          932 c          966 g          536 t
ORIGIN      
        1 gcgcggctcg atctcttccg gcacacccaa cccggtgtcg atcacggcga agcgcacgaa
       61 gtccgcgccg tctttggcga aggtctcgac ccggatttcg acgccgccgc tgtcggtgaa
      121 tttgagggcg ttgtcggtga gtttgagcag agcctggcgc acccgtctcg gatcagcggc
      181 ggcgcgcggc acgctggccc ccgcggtgac ggtcaggcgc aagccctttt gccaagccag
      241 cggctggcac aagcgcgtca cggcgcgcgc ggcctgcacc ggatcgcatt ccttgtccga
      301 caggcggtcg ctgtcgtcct gggtcagggc gatgacgtct tcgagcagcg tctgcaagcc
      361 gcggcccgat tccagcatca ccttcacatg atcgccgtgc ggctggccga gttcggcgcg
      421 gtcgagcagt tgcgccatgc cgagcagcgc gttgagcggc gtgcgcagtt catggctgat
      481 gttggcgatg aagatggatt tcgctttggc gccggcctcg gcgtcgtcgc gggccgcttc
      541 ggcatcgccc ttctccgtcg acattttctc tatcaggtct ttgttttcca ggcgcaggcg
      601 gatgctttcg tcgagcagcc gcgccatgcc gcggcaatag ccggccagca ccccggcgaa
      661 gcagaacacc agcaccgccg tcatgtcgct gtacaggctg ccttcgatca gcagcatggc
      721 gatcaagggg ccgagagaga agaaggcatg cgcgagatag gcgcggcggt gcgcggagcg
      781 ggcgatgaat tcggtggccg tcatgcccat aaaggcgagg cagagatagg cttgggccgc
      841 gaacgagtcc ggcacgaacc agaagaagac gccgacgccc cagcttgccc cggcgatcgc
      901 cgagatgaag gtgtagcgcc gggcccaata ttgcgggctg gtgcccggtg cgcggtgctt
      961 gtaggtctgt gtcaactgct ccgcgacaat ggcgacgacg atcagcaaca ccagcggcag
     1021 gaacggaaga atggtcagag ggacgccggc gaacagcgtc gccgacacgg ccagagcggc
     1081 gaagggcagg gagaggtaat ggcgtccgag cgcgtatagt atgtcgatgc ggcccgcgaa
     1141 aatgccgcga tctccatcct tgcagacttc aggttcagca gacattgacg gtatcccttt
     1201 cccaagacta gctttccctg cctttccaaa cgcttaaggt ttcgtgatga cgtccagtcc
     1261 ctccctgcat gcagaagcgc tcgccgcccg cgcctggccg ttcgaggagg cgcgtaagct
     1321 gttggcgcgg atggagaaac gcggaagcgg gcgcgaggtc atcttcgaga ccggttacgg
     1381 cccttcgggc ctgccgcata tcggtacctt cggcgaagtg gcccgcacca cctgggtgcg
     1441 gcgcgcattt tctctgatgt cgggcgccaa gacgcggctg ctcgcttttt ccgacgacat
     1501 ggacggactg cgcaaagtcc ctgccaatct gcccaaccag gagatgctgg ccgcgcatct
     1561 gggcaagccg ctgaccgcca tccccgatcc gttcggcacc catgatagct tcggcgccca
     1621 caacaatgcg cggctgaagg cgttcctcga ccgcttcggt ttcgattacg aattcctcag
     1681 ctccaccgac tgctacaaga gcgggcgctt cgatgaggcg ctgctgaccg tgctggccca
     1741 ttacgagaag gtgaaggacg tcgtgctgcc gacactgggg ccagagcggc gcgccaccta
     1801 ttcgcccttc ctgccgattt cgcccaagac cggcatagtc ctgcaagtgc cgatcctgga
     1861 atgggatgcc aagcgcggca ccatcgtctt cgaggaagac ggcaagaagc aggagcttcc
     1921 cgtcaccggc ggcaacgtca agctgcaatg gaaggcggac tgggcgatgc gctggctggc
     1981 gctcggcgtc gattacgaga tggcgggcaa ggatttgatt tcctccgtcg aattgtcgtc
     2041 gaagatcgtg cgcgccatcg gcggggcgcc gccggaaggc ttcaattacg aactgttcct
     2101 cgacgatcag ggccagaaga tttccaagtc gaaaggcaac ggcctgtcgg tggacgaatg
     2161 gctgacctac ggcaccgagg agagtctggc gctgttcatg tttcagaaac cgcgcgcggc
     2221 caagaaactc tatttcgatt ccatcccgaa gacggtggac gattacatcg cgctgctcga
     2281 tgcctatcgc gccaaggaga agagcgccgc cgagcgcctc gaaagcccga tctggcatat
     2341 ccacgccggc acgccgccgg acgagcgtta tccggtcagc ttcgcgctgc tgctcaatct
     2401 ggtcagcgcc tcgaacgcgc acaatcgcga agtcttgtgg ggcttcatcc agtcatacgc
     2461 gccgcaggcc aatcctgagg ctaatcccgg cctcgataag ctggtcggct acgcgatcaa
     2521 atattacgac gatttcgtca agccgcagaa gcgctaccgc gcgcccagcg acaaggaacg
     2581 cgccgcgctg gccgacctcg cggacaagct gcgcgccttc ggcgccgagc gcgacgccga
     2641 gaaggtgcag ttcgaagtct acgaggtcgg caagcgtcac gacttcgaac cgttgcgcga
     2701 ctggttcaaa gcgctgtatg aagtgctgtt cggccagagc gcgggtccgc gcttcggctc
     2761 tttcgccgcg ctgttcggct gtgccgagac tgcggcgttg atcgaacgcg cgctccaagg
     2821 agaatttgtt tcggccgcat gaggtacgtc cttgccctcg cttttctgct ggcggtgccg
     2881 gcttacgcgg acaccgcaga caagctttac gccgacggca aatatgagga agcggtcaag
     2941 accggcttgg ccgagaacaa cgcggcgggt tttgcagcgg ccgcac
//
LOCUS       DASWPU010000442         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_101490,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000442 DASWPU010000000
VERSION     DASWPU010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101490"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VGC27_09460"
     CDS             <1..796
                     /locus_tag="VGC27_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0282836.1"
                     /translation="ASAVNLGMHGRHKPGIGQSFWQFHHYRPGDAANAIDWRQSAKSQ
                     HLYVREREWEAAQSVWLWRDGSPGMRFASRGERKIDRASLLALALGVLLVRGGERIAL
                     FGEPEAPANSRAAYRRIGYALAEREPADNAVPPERPLPRNAQFVWFGDFLAPLGEIEA
                     AIRRLTRHNAGGRLVHIVDPAEEDFPYAGRTRFEAVNGTDSRILGRAESVAPDYRARF
                     KAHSEAVALLARKLGWNYMAHRTDRSPQTAMAALYADLGGGQRLRT"
     gene            803..>2968
                     /locus_tag="VGC27_09465"
     CDS             803..>2968
                     /locus_tag="VGC27_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEY0282837.1"
                     /translation="MESLANISFGAPWVLLALALLPAIWFLLRVIPPLPKRVVFPPLA
                     LLLGLRNEEQTPAGTPWWLMLLRLMAAAALIVALAEPQIGRALELAGNGPIVLFVDNG
                     WTAAPDWKARQGLAEEAVRLAARQRRPVAIVTTADKPDTGLMDAGRAGRAVRALTPKP
                     WLADRSDALAALAKVKFAAPEILWLSDGVDDGHARATAQTLSRLGHLRIFAGRQGPLA
                     VTSIVNTADGFEAELLRTGTSGTRQGAATAFGDQGQILGTANFRFDDGKNKTTARLAL
                     PLEMRNATARIAINGEDSAGAVHLLDRGAPRRAAGLVSASNPGGEEPLLSGLYYLQRA
                     LAPYADTRKGTISEVLSRGISVLILSDIGKIAGDDHARVKQFVENGGLLIRFAGERMA
                     GGTDDLVPVELRSGGRYLGGALAWAAPQHLAPFPDNSPFGGLSIPGDVSVSRQILAEP
                     SVELASHTWARLTDGTPMVTAARRGKGWIVLFHVTAGPGWSTLPLSGLYVSMLQRLLS
                     LSAGTDPHDMTANASLPATFTLDGFGHLAAASAEAQPVSGAQIATIEPSRLHPPGLYG
                     TPGAERALNAASGNTVLLPFGALGTRVSFYAARASVALQAPLLLFAALLLLADFAISL
                     VLRGYAPRLGKLGRGTAALLGAFLLLHPGEARADDAFDLKAALDTRLAYVITGVPDTD
                     AMSKAGLTGLGAALAARTSYVPKEPMGVDLAKDDLALFPL"
BASE COUNT          438 a         1031 c         1008 g          491 t
ORIGIN      
        1 ggcgtcggcg gtgaatctcg gcatgcatgg gcggcacaaa cccggcatcg gccagagctt
       61 ttggcagttc catcattatc gccccggcga tgcggcgaac gccatcgact ggcgccaatc
      121 ggccaagtct cagcatctct atgtgcgcga gcgcgaatgg gaagcggcgc aatccgtctg
      181 gctgtggcgc gacggctcgc ccggcatgcg ctttgcctcg cgcggcgaac gcaagatcga
      241 ccgcgccagt ctgctggcgc tggcattggg cgtgctcttg gtgcgcggcg gcgaacgcat
      301 cgccctgttc ggcgaaccgg aagccccggc caattcgcgc gccgcctacc ggcggatcgg
      361 ttatgcgctc gccgaacgcg aacccgccga caatgcggtg ccgcccgaac ggccgctgcc
      421 gcgcaacgcg caattcgtct ggttcggcga tttcctggcg ccgctcggcg agatcgaagc
      481 cgcgatccgc cgcctgacgc gccacaatgc cggcggacgg ctggtgcata tcgtcgatcc
      541 ggcggaagag gattttccct atgccgggcg cacccgcttc gaagcggtaa acgggacgga
      601 cagccgcatc ctggggcggg cggaaagcgt ggcgccggac taccgcgcgc gcttcaaggc
      661 gcatagcgaa gcggtggcgt tgttggcgcg caagctcggc tggaattaca tggcgcaccg
      721 caccgaccgc tcgccgcaaa ccgccatggc ggcgctctat gccgatctcg gcggcggaca
      781 aaggctgcgg acctgaccgc gcatggaatc gctggccaac atcagtttcg gtgcgccatg
      841 ggttttgctg gcgctcgcct tgctgccggc gatctggttc ctgttgcgcg tcatcccgcc
      901 cttgcccaag cgcgtggtgt ttccgccgct ggccctgctg ctgggattgc gcaacgaaga
      961 acagactccg gcgggcacgc cctggtggct gatgctgctg agactcatgg cggcggcggc
     1021 gttgatcgtg gcgctggccg agccgcagat cggccgcgcg ctggaacttg ccggcaacgg
     1081 ccccatcgtt ctgttcgtcg ataatggctg gaccgccgcg ccggattgga aagcccggca
     1141 gggactggcg gaagaagccg tacgcctcgc cgcccgccag cgccggcccg tcgccatcgt
     1201 tacgaccgcc gacaagccgg acacgggtct catggacgcg ggccgcgccg gacgcgccgt
     1261 gcgcgccctg acgcccaaac cctggctcgc cgaccgcagc gatgcgcttg ccgctctggc
     1321 gaaagtgaaa ttcgccgcac ccgagatcct ctggctctcc gatggggtcg acgacggcca
     1381 tgcgcgcgct accgcccaaa cgctttcgcg cctcggccat ttgcggattt tcgccggccg
     1441 ccaaggcccg ctggcggtca cttcgatcgt caacacggcg gacggcttcg aggcggagct
     1501 gctgcgcacc ggcaccagcg gcacgcgcca aggcgcggcc actgcttttg gcgatcaagg
     1561 ccagattctc ggcaccgcga atttccgttt cgacgacggc aagaataaga ccacagcgcg
     1621 cctcgccctg cctctggaga tgcgcaacgc caccgcgcgc atcgccatca atggagagga
     1681 cagcgcaggc gcggtgcatc tgctcgaccg gggggcgccg cggcgcgccg ccgggcttgt
     1741 ttccgccagc aatccgggcg gcgaggagcc gttgctgtcc gggctttatt atctccaacg
     1801 cgcgctggcg ccatatgccg acacgcgcaa aggcacgatc tccgaggtgt tgtcacgcgg
     1861 tatctcggtg ctcatcctgt cggacatcgg caagatcgcc ggcgacgatc acgcgcgcgt
     1921 caagcaattc gtggagaatg gcggactgct gatccgcttc gccggcgagc gcatggcggg
     1981 cggcaccgac gatctggtcc cggtggaact gcgcagcggc gggcgttatc tcggcggcgc
     2041 gctggcctgg gcggcgccgc aacatctggc gccgttcccc gacaattccc ccttcggcgg
     2101 actttcgatt cccggcgacg tgtcggtgtc gcggcaaatc ctggccgaac cgtcggtcga
     2161 gcttgcctcg catacctggg cgcggctcac cgacggcacg ccgatggtca cggcggcgcg
     2221 gcgcggcaag ggctggatcg tgctgttcca cgtcaccgca gggccgggct ggtccacgct
     2281 gccgctgtcg gggctttatg tcagcatgtt gcagcgactg ctgtcgctgt cggccggaac
     2341 cgatccgcac gacatgacgg cgaacgccag cctgcccgcc acgttcactc tcgacggctt
     2401 cggccatctg gcggcggcca gcgccgaggc ccaaccggta agcggcgcgc aaatcgcgac
     2461 catcgagccg tcgcgcctgc atccgcccgg actttacggc acgccaggcg ccgaacgggc
     2521 gctgaacgcg gcgagcggca atacagtgct gctgccgttc ggcgcgctgg gcacgcgcgt
     2581 ttcgttctac gccgcgcgcg cctccgtcgc cttgcaggcg ccgttgctgc tgttcgcggc
     2641 gctgctgctg ctcgccgatt tcgcgatttc gctggtgctg cgcggctacg cgccgcggct
     2701 tgggaagctt gggcgcggca ccgctgccct gctcggcgcg ttcctgctgt tgcatccggg
     2761 agaggcgcgg gccgacgacg ctttcgatct caaagcggcg ctcgacacca gactcgccta
     2821 tgtcatcacc ggcgttcccg acaccgatgc gatgagcaaa gcgggactga cggggctggg
     2881 cgccgcgctg gcggcgcgca cctcctatgt gccgaaggag ccgatgggcg tcgatctcgc
     2941 gaaggacgat cttgccctct tcccgctg
//
LOCUS       DASWPU010000443         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_9406, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000443 DASWPU010000000
VERSION     DASWPU010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9406"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(733..1611)
                     /gene="sucD"
                     /locus_tag="VGC27_09470"
     CDS             complement(733..1611)
                     /gene="sucD"
                     /locus_tag="VGC27_09470"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010327286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY0282838.1"
                     /translation="MSILVDKNTAVICQGFTGKQGTFHSEQAIAYGTKMVGGTTPGKG
                     GSTHLGLPVFDTVRQAKEATGCTASAIYVPPPFAADAILEAVDAEIPLIVCITEGIPV
                     LDMVKVKRALTGSKSRLVGPNCPGVITPGECKIGIMPGHIHRRGTVGIVSRSGTLTYE
                     AVWQTTAVGLGQSSCVGIGGDPVKGTEFVDVLEMFLADPETESIVMIGEIGGTAEEDA
                     ADFLKRSKTKKPIVGFIAGVTAPPGRRMGHAGAIISGGKGDAASKIEAMKSAGIRVSP
                     NPAALGTTLVELLKEK"
     gene            complement(1612..>2121)
                     /gene="sucC"
                     /locus_tag="VGC27_09475"
     CDS             complement(1612..>2121)
                     /gene="sucC"
                     /locus_tag="VGC27_09475"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HEY0282839.1"
                     /translation="ALFRHPEIAVALRDLTEEDAKEVEASKYDLNYVTLDGTIGCMVN
                     GAGLAMATMDIIKLTGGEPANFLDVGGGASKEKVTEAFKLILSDKNVKGILVNIFGGI
                     MKCDIIADGIVAAAKEVNLGVPLVVRLEGTNVEKGKDILARSGLPIVSGNDLADAAQK
                     IVKAVKEAA"
BASE COUNT          366 a          633 c          682 g          440 t
ORIGIN      
        1 ccggcgaagt atgccgccca ggaaggttcg accgaatccg ggttttcctg atattgggcg
       61 tagagcgcct cgacataggc ggcattggct ccgaacagga acgaggtcgc ctcgaggatg
      121 tcgttggagg agcggttgag ccgttctgga gtggattggc ttgtcatttc agtcagttaa
      181 tggccttcgc ttattgggtc tcaagcccgt ccggggtgaa ttagaatagt tcaaagaagg
      241 gcatgtaccg catccgggca tcgaattcca tgcccaaaca cgacatgaaa caacccaccc
      301 ggtaaaaata ggttcaatga gactgagttg cgattacggg caggcttaaa gcggcttttc
      361 gcttgacgcc ctgcggggcg tggccgcgcc gtcgatcgta atttggcact ggcaacattt
      421 tcgccgtcag cgacacttgg caggcggtct ggcaccgcga gtcccggcct tggctgctcc
      481 gctctatctg gaaggctggg cgcatcttct atgaaaggcc gatgcaggcg ctgatccgca
      541 gcttgccggc acggttcgcc ggaaagggcg cgactggctg aggtccttca cccttcgaca
      601 agctcagggt gagggttgag ctgacccctc atgctgagct tgtcgaagca tgagcttgtc
      661 gaagcatgag cttgtcgaag catgagcttg tcgaagcatg agcctgtcga agcatgcggg
      721 ctgcaaagtg ttctattttt ccttcagcag ttccaccagc gtggtcccaa gcgcggccgg
      781 attgggcgaa acgcggatgc cggcggattt catcgcctcg atcttcgaag ccgcgtcgcc
      841 cttgccgccg gagatgatgg cgccggcatg gcccatgcgc cggccgggcg gggcggtgac
      901 gccggcgatg aatcccacta ttggcttctt cgtcttggag cgcttgagga aatcggccgc
      961 gtcttcctcg gcggtgccgc cgatctcgcc gatcatcacg atgctttcgg tttccgggtc
     1021 ggccaggaac atctccagca catcgacgaa ctcggtgcct ttcaccggat cgccgccgat
     1081 gcccacgcaa gaggattggc ccagccccac cgccgtggtc tgccacaccg cctcataggt
     1141 cagcgtgccg gagcgcgaaa cgatgccgac cgtgccgcgg cgatggatgt gacccggcat
     1201 gatgccgatc ttgcattcgc ccggcgtgat gacgccgggg cagttggggc cgacgaggcg
     1261 cgatttcgaa ccggtcagcg cgcgcttgac cttgaccatg tcgagcacgg gaatgccttc
     1321 ggtgatgcag acgatcagcg gaatttccgc gtcgacggct tccaggatgg cgtcggcggc
     1381 gaagggcggc ggtacgtaga tcgcgctggc ggtgcagccg gtggcctcct tggcctggcg
     1441 caccgtgtcg aacaccggca ggccgagatg cgtcgagccg cctttgccgg gcgtggtgcc
     1501 gcccaccatc ttggtgccgt aggcgatggc ctgctcggaa tgaaaggtgc cctgcttgcc
     1561 ggtgaagccc tggcagatga cggcggtgtt cttatcgacg aggatggaca tctatgctgc
     1621 ttccttcacc gctttgacga tcttctgcgc cgcgtcggcg aggtcgttgc cggagacgat
     1681 gggcaggccg gacctggcca ggatgtcctt gcccttctcc acattggtgc cttccaggcg
     1741 caccaccagc ggcacgccga gattcacttc tttagccgcg gcgacgatgc cgtccgcgat
     1801 gatgtcgcat ttcatgatgc cgccgaagat gttgaccagg atgcctttca cgttcttgtc
     1861 cgacaggatc agcttgaacg cctcggtgac cttttccttg gaagcgccgc cgccgacatc
     1921 gaggaagttg gccggctcgc cgccggtgag cttgatgatg tccatcgtcg ccatggccag
     1981 gccggcgccg ttgaccatgc agccgatggt gccgtcgagg gtcacgtaat tgagatcgta
     2041 tttggacgcc tcgacttcct tggcgtcctc ctcggtcaaa tcgcgcaacg ccaccgcgat
     2101 ctcagggtgg cggaacagcg c
//
LOCUS       DASWPU010000444         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_28025,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000444 DASWPU010000000
VERSION     DASWPU010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28025"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VGC27_09480"
     CDS             complement(<1..747)
                     /locus_tag="VGC27_09480"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282840.1"
                     /translation="MKEKFDPPGSQTINKKTVKPVIAKYRNRADVDAAFARLGAYWDG
                     LLGICQVSTPDIHTDRMVNVWNAYQCMATFNMSRSASTFESGIGRGLGFRDSNQDLLG
                     FVHMVAECARERILDLAATQLPTGSAYHQYQPLTKKGNDGVGGNFNDDPHWLIIGVAA
                     YLKETGDWSILDEPVVFDNTPGSERPLYEHLGRSIRYTLERLGPHGLPLIGRADWNDC
                     LNLNCFSEEPGQSFQTTTSKEGKVTESVFIA"
     gene            complement(744..1556)
                     /locus_tag="VGC27_09485"
     CDS             complement(744..1556)
                     /locus_tag="VGC27_09485"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282841.1"
                     /translation="MKYGYFDDENKEYVITRPDTPLPWINYLGCQNYFGILSSTAGGY
                     SYYRDARLRRITRGRYNNVPLDFGGRYIYLRDNESGEYWSPSWMPTMGEHEDFVCRHG
                     MGYSIISSTYRGIAACTRYFVPLDENLEIWQLTLTNNRPAPVELSVFSSVEFCLWDAQ
                     DDSTNFQRNFSIGQVEVDDEVIYHKSEYRERRDHFAWFACSEPLAGFDTQREAFLGAW
                     RGWDEPSPSNEANRPTPSPMAGRRSARTMCASHLRPARRIRSYSFSATTRIR"
     gene            complement(1553..>1852)
                     /locus_tag="VGC27_09490"
     CDS             complement(1553..>1852)
                     /locus_tag="VGC27_09490"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEY0282842.1"
                     /translation="HWWVQAEAVIGFYNAYQISGETRFLVAARQAWDYIETKMVDRVH
                     GEWHAKLTPQGAPLTAAEDPDACLAGPWKCPYHNARVCYEMIERLEKQASGRLAS"
BASE COUNT          335 a          542 c          598 g          377 t
ORIGIN      
        1 ggcgatgaac acggattcgg taactttgcc ttccttgctg gtcgtggtct ggaaagattg
       61 tcccggctcc tccgaaaagc agttgaggtt caggcaatcg ttccagtcgg cgcggccgat
      121 cagcggcagg ccgtgcgggc ccagccgttc gagcgtgtag cggatgctgc gccccagatg
      181 ctcgtagagc ggcctctcgc ttcccggagt gttgtcgaag acgacgggtt cgtcgaggat
      241 gctccagtcg cccgtttcct tgagataggc ggccacgccg atgatcagcc aatgcggatc
      301 gtcgttgaaa ttgccgccga cgccgtcgtt ccccttcttg gtgagcggct ggtactggtg
      361 ataggcgctc ccggtcggca actgcgtcgc cgccaggtcg agaatgcgct cgcgggcgca
      421 ctcggcaacc atgtgcacga aaccgagcag atcctggttg gaatcgcgga agccgaggcc
      481 gcggccgatg ccggattcga aggtcgatgc ggaacgcgac atgttgaacg tcgccatgca
      541 ttgataggcg ttccagacgt tgaccatgcg gtcggtgtgg atgtcgggcg tcgacacctg
      601 gcagattccg agcaggccgt cccaataggc gcccaggcgc gcgaaggcgg catcgacgtc
      661 ggcccggttg cgatacttcg cgatcaccgg cttcacggtc ttcttgttga tggtctgcga
      721 gcccggcgga tcgaatttct ccttcaccgg attctcgtgg tagccgagaa cgaatatgat
      781 ctgatgcgtc tcgccgggcg caagtgcgac gcgcacatgg tgcgagccga tcggcgacca
      841 gccatgggcg atggagttgg acgatttgcc tcgttcgacg gcgacggctc gtcccagccg
      901 cgccaggcgc cgaggaaagc ctcgcgctgc gtatcgaacc cggcgagcgg ctccgaacag
      961 gcgaaccagg cgaaatggtc gcgccgctcg cgatattcgc tcttgtggta gatcacttcg
     1021 tcgtcgacct ccacctggcc gatgctgaaa ttgcgctgga aattggtgga atcgtcttgc
     1081 gcatcccaca agcagaactc gaccgacgaa aagaccgaca gctcgaccgg cgccgggcgg
     1141 ttgttggtga gggtgagctg ccagatctcg aggttttcgt cgagcggcac gaaatagcgc
     1201 gtgcacgccg cgatgccgcg ataggtcgag gaaatgatgc tgtagcccat gccgtgccgg
     1261 cagacgaagt cttcgtgctc gcccatggtg ggcatccagg acggcgacca gtactcgccg
     1321 ctctcgttgt cgcgcagata gatgtagcgt ccgccgaaat cgaggggaac attgttgtag
     1381 cggccgcgcg taatgcggcg caggcgcgca tcccggtaat aggaatagcc gcccgcggtg
     1441 ctggagagaa ttccgaaata gttttggcat cccagataat tgatccacgg cagcggcgta
     1501 tccggcctgg tgatgacgta ttctttattc tcatcgtcga aataaccgta cttcatgacg
     1561 ccaatcttcc gcttgcttgc ttttccagcc gctcgatcat ttcgtagcag acgcgggcat
     1621 tgtgataagg gcacttccag gggccggcga gacaggcgtc cgggtcttct gctgcggtga
     1681 ggggcgcgcc ctgcggggtg agttttgcgt gccactcgcc atggactctg tccaccattt
     1741 tcgtttcgat atagtcccag gcttggcgcg cggccacgag aaagcgcgtc tcgcccgaaa
     1801 tctggtaggc attgtagaag cctatgaccg cctcggcctg cacccaccag tg
//
LOCUS       DASWPU010000445         5073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_120397,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000445 DASWPU010000000
VERSION     DASWPU010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120397"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VGC27_09495"
     CDS             <1..325
                     /locus_tag="VGC27_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY0282843.1"
                     /translation="ERNRVHLAKMLKSGANLLLLDEPTNDLDVETLRALEMALEEFAG
                     CAMIISHDRWFLDRIATHMLAFEGDSHVEWFEGNFQDYEADKKRRLGIDADQPHRIKY
                     KRFKR"
     gene            complement(327..731)
                     /locus_tag="VGC27_09500"
     CDS             complement(327..731)
                     /locus_tag="VGC27_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0282844.1"
                     /translation="MTLVDSNVLVDVLRGDPQWLTWSIEQINSRASLGPVYFNEIVYS
                     ELAVKSASESELDRSLTDAGLLLLPIPKSAYFLAGKIFGRYRSAGGIRTSNLPDFFIG
                     AHAQVAGLPILTRDVGRFRTYFPKVELIAPDV"
     gene            complement(728..991)
                     /locus_tag="VGC27_09505"
     CDS             complement(728..991)
                     /locus_tag="VGC27_09505"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0282845.1"
                     /translation="MATTVTVKGQVTLPKKVREAAGIRPGDRVEVRATAAGGIVIEKP
                     AAKSDYKARLYEIAKRGVIRDITTDELMDMTRGEMDPLTGIRK"
     gene            complement(1077..1736)
                     /locus_tag="VGC27_09510"
     CDS             complement(1077..1736)
                     /locus_tag="VGC27_09510"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0282846.1"
                     /translation="MSNLSLEQNILGAMRPFRLVFAALIFAAGFAGFSAPAQAATPAE
                     QYITDNVQKGLLILTDKALNKDQRKTQFQDFLIGLTDIKVIADYTLGQYRRSASPADL
                     AAFDDAFKTYALAVYQSYFNKFSGQTLQVTGSYPLAADESVVKTIMIDPKKPNDPKPM
                     QVNFRVVKTGGRLAVIDFSVEGVWLREMERSEFTSYLGQHNGDLSSLTASLTKKAKTI
                     K"
     gene            2204..3172
                     /locus_tag="VGC27_09515"
     CDS             2204..3172
                     /locus_tag="VGC27_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0282847.1"
                     /translation="MERLVAMLRAAGDPTRLRLLLLLRQAELTVSELIEIVGQSQPRV
                     SRHLKLLCGAGLIERFKEGSWVFYRAADEGRGAALGATLSELAEGPHHTDLKRLAAVR
                     EARAAEAAAFFKANASEWERIRAFHAPEKDVEAAIVRHLGTAPIEHLLDAGTGTGRML
                     ELLAPQVKHAIGVDVSPEMLAIARDRLMRANLAQCQVRQGDTYRLPYPSGGLLAGFDA
                     VLFHQVLHYLDDPGAAVAEAARVMRAGGRLLIADFAPHELEFLRQDFAHRRLGFSDRE
                     VKGWFAAAGLKPLAAEAIAPNSDAAEKLTVKIWLAASPAKTRAEAA"
     gene            3169..4053
                     /gene="metF"
                     /locus_tag="VGC27_09520"
     CDS             3169..4053
                     /gene="metF"
                     /locus_tag="VGC27_09520"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEY0282848.1"
                     /translation="MSLKDLVADSRPIGVSFEFFPPSTAEMEERLWRAVKRLEPLRPA
                     FVSVTYGAGGSTRERTHATVKRIVEETALKPAAHLTCVAAARSEVDDVVRSYWQAGVR
                     HIVALRGDMPEADAPFVTHPQGYGSTPELIAGIRAIGAFNISVSCYPERHPDSPSVEH
                     DLDLLKRKAEAGASRAIGQFCFDNDAIARLRDRADRWGISVPIIPGLMPSTNFKSVAR
                     MAARCGASIPSWLARLYEGLDDDLESRRIVASAVLAEQVTRLHAYGFDQFHFYTLNQA
                     DLTYATCRILGLVPKDLS"
     gene            4050..>5073
                     /locus_tag="VGC27_09525"
     CDS             4050..>5073
                     /locus_tag="VGC27_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY0282849.1"
                     /translation="MSFDRAARIRWLKEEAQRRILLLDGSWGVMIQGYKLGEDDFRGA
                     RFGNHTHDLKGNNDLLTLTKPEVIREIGDAYLAAGADFIETNTFTATETSQADYGLAH
                     LVGELNFAGAKLAREICDARTTADRPRLVAGVLGPTNRTASISPDVNDPAFRNITFDA
                     LRRTYREAALGLIKGGADLLMIETIFDTLNSKAAIFAIEEAFEETGERLPVWISGTIT
                     DLSGRTLTGQTVEAFWHSVRHAQPFAAGLNCALGAKELRQYVAELSAVADTLVSTHPN
                     AGLPNEFGGYDDTPEEMAEKIGEFARSGLVNIVGGCCGTTPAHIKAFAEAIDGVPPRA
                     VPEKPRY"
BASE COUNT          905 a         1640 c         1586 g          942 t
ORIGIN      
        1 cgaacgcaac cgcgtccatc tcgccaagat gctgaagtcg ggcgccaatc tgctcctgct
       61 cgacgagccg accaacgatc tggacgtcga gaccttgcgg gcgctggaaa tggcgctgga
      121 ggaattcgcc ggctgcgcca tgatcatcag ccatgaccgc tggttcctgg accgcatcgc
      181 cacccacatg ttggccttcg agggcgacag ccatgtcgaa tggttcgaag gcaacttcca
      241 ggattacgaa gccgacaaga agcgccgcct cggcatcgac gccgaccagc cccaccgcat
      301 caagtacaag cggttcaagc ggtgattcaa acgtcgggcg cgatcagttc gaccttgggg
      361 aaataggtgc gaaagcgccc cacgtctcgg gttaggatcg gaagtccggc aacctgcgca
      421 tgcgcaccga tgaagaaatc gggaagattg ctggtgcgaa tgccgccggc gcttcggtac
      481 cgaccgaaga ttttcccggc gagaaagtac gcgctcttag gtatcggcag cagcaggaga
      541 ccggcgtccg tcaacgaccg gtcgagttcg gattcactag cgcttttcac cgcgagttcg
      601 gaatacacga tctcgttaaa atataccggg ccgaggctgg cacgactgtt gatctgttcg
      661 atggaccacg tcagccattg cggatcgccc ctcagaacgt ccaccaggac gttgctgtcg
      721 acgagcgtca tttccggatt ccggtcaacg gatccatctc gccgcgcgtc atgtccataa
      781 gctcatcggt cgtaatgtcg cgaatgacac cccgttttgc gatttcataa agccgcgcct
      841 tgtagtcgct cttcgccgcg ggtttctcga tcacaatccc gcccgccgcc gtagcgcgca
      901 cttccacgcg gtcgcccggc cggatgcccg ctgcttcccg gactttcttg ggcagggtga
      961 cttggccctt caccgtgacc gtcgtcgcca tttccacctc cgtattactt ttcaaaaaag
     1021 taatacctcg acggcagaaa gtcaatcctg ggatgccgct gccgccccga tgaagcctat
     1081 ttgatcgtct tggccttctt cgtcaggctg gcggtcaggc tggagaggtc gccgttgtgc
     1141 tggccgagat aggaggtgaa ttcgcttctc tccatttcgc gcagccacac gccttcgacc
     1201 gagaaatcga tcaccgccag ccgtccgccc gtcttgacga cgcggaagtt cacttgcatc
     1261 ggcttcggat cgttcggctt tttcggatcg atcatgatcg tcttgacgac gctttcgtcc
     1321 gccgccagcg gataggaacc cgtcacttgc agcgtctggc cggagaattt gttgaaatag
     1381 ctctgataga ccgccagcgc gtaagtcttg aacgcgtcgt cgaacgcggc caggtcggcg
     1441 ggcgaggccg agcgccggta ctggcccagc gtgtagtcgg cgatcacctt gatgtcggtc
     1501 agcccgatca ggaaatcctg aaactgcgtc ttgcgctgat ctttgttcag cgccttgtcg
     1561 gtcaggatca acagaccctt ctgcacattg tccgttatgt actgttcggc aggggtggcc
     1621 gcctgtgccg gcgccgagaa acctgcgaaa cccgccgcga atatgagggc cgcgaacacc
     1681 agccggaacg gccgcatcgc gcccaggatg ttttgttcta aagaaagatt agacatcggc
     1741 aatgaacctc tggctacgcc tcacaggtaa tggtcgcttg tgcttatggc aagttcgtgg
     1801 cggccgtttt gtcttctgaa ggcggccgcg ctcccgtctc tccgttgcgg atttcgcgcc
     1861 ccctgtattg cctgtaaagg tcgggggtcg cggcgtaata atccagcgaa tcgcgctcga
     1921 tgcggcggcg aaggtccaag caggggcaga acaagataag accctttgtc cccaccccag
     1981 accgccaggg tccggccgga atcctcgcta tgttcgggaa ttcccagccg taattgccgc
     2041 cggaaacgcg aaatccgcat ctgcccacac gcccaggttt aaatccgtta aggccctgac
     2101 cggggtcgtc cccgtgggca tccgcttaac ctcatttccc gttcgagtct tgcgccctcg
     2161 tatcatataa agatatgttt atatgtgttg aaaggaggaa gagatggaga gactagtcgc
     2221 catgttgcgc gccgccggcg atccgacccg cctgcgcctg ctcctcttgc tgcgccaggc
     2281 ggagctgacg gtcagcgagc tgatcgagat cgtgggccag agccagccgc gggtctcgcg
     2341 ccatctgaag cttttgtgcg gcgccggtct gatcgagcgg ttcaaggagg ggagctgggt
     2401 cttctaccgg gcggccgacg aaggacgggg tgcggcgctc ggcgccacgc tgtccgaact
     2461 ggccgaggga cctcaccaca ccgatttgaa acggctggcg gcggtgcgcg aagcgcgcgc
     2521 cgcggaagcc gccgcgttct tcaaggccaa tgcttcggaa tgggaacgca tccgtgcgtt
     2581 ccacgcgccg gaaaaggatg tcgaagccgc catcgtccgt cacctcggga cggcgccgat
     2641 cgagcatctg ctggatgccg ggaccggaac cgggcgcatg ctggaattgc tggcgccgca
     2701 ggtgaagcac gccattggcg tcgacgtcag cccggagatg ctggcgatcg cgcgcgaccg
     2761 cctgatgcgg gcaaacctgg cgcaatgtca ggtgcgccag ggcgacacct atcgcctgcc
     2821 ttatccgtcg ggcggcctgc tggccgggtt cgatgcggtg ctgttccacc aagtgctgca
     2881 ttatctcgac gatcccggcg ccgccgtggc ggaagccgcg cgcgtgatgc gcgccggcgg
     2941 ccggctgttg atcgccgact tcgcgccgca cgaactggaa ttcctgcgcc aggatttcgc
     3001 ccatcgccgg ctgggttttt ccgaccgcga agtgaagggc tggttcgccg ccgccggatt
     3061 gaagccgctg gccgccgagg ccatcgcgcc gaattccgat gccgcggaaa aactgacggt
     3121 gaagatttgg cttgcggcgt cgccggccaa aacccgcgcg gaggccgcat gagtctcaag
     3181 gatttggtcg ccgacagccg cccgatcggc gtgtcgttcg agttctttcc gcccagcacc
     3241 gcggagatgg aggagcggct gtggcgtgcg gtaaagcggc tggagccgct ccgtcccgcc
     3301 tttgtctcgg tcacctacgg cgccggcgga tcgacgcgcg agcgcaccca cgccacggtc
     3361 aagcgcatcg tggaagagac ggcgctcaag cccgccgcgc atctcacctg cgtcgccgcc
     3421 gcgcgaagcg aggtggacga cgtcgtacgc tcctattggc aggcgggcgt gcgccacatc
     3481 gtggcgctgc gcggcgacat gcccgaggcc gatgcgcctt tcgtcacgca tccgcaaggc
     3541 tatggctcga cgcccgagct gatcgcgggc atccgcgcca tcggggcatt caacatcagc
     3601 gtgtcctgct atcccgagcg ccacccggac tcgccgtcgg tcgagcacga tctcgacctg
     3661 ctcaagcgca aggccgaggc gggcgcctcg cgcgccatcg ggcaattctg cttcgacaac
     3721 gacgccattg cgcggctgcg cgaccgcgcc gaccgctggg gcatttcggt gccgatcatt
     3781 ccgggcctga tgccgtcgac gaatttcaag agcgtggcgc ggatggcggc gcgctgcggc
     3841 gcgagcattc cgtcctggct ggcgcgcctc tacgaaggcc ttgacgacga cctcgaaagc
     3901 cggcggatcg tcgcctcggc ggtactggcc gaacaagtga cgcggctgca cgcttacggc
     3961 ttcgaccagt tccacttcta taccctcaac caggccgacc tgacctacgc gacgtgccgg
     4021 atattgggtt tggttccaaa ggatctgtca tgagtttcga ccgcgccgca cgcatccgct
     4081 ggctgaagga ggaggcgcag aggcgcatcc tgctgctcga cggctcctgg ggcgtgatga
     4141 tccagggcta taagctgggc gaagacgatt tccggggtgc gcgcttcggc aatcatacgc
     4201 acgacctgaa gggcaacaac gatcttctga ccctgaccaa gcccgaggtg atccgcgaga
     4261 tcggcgacgc ctatcttgcc gccggcgcgg atttcatcga aaccaacacc ttcaccgcga
     4321 cggagacctc ccaagccgat tacgggctgg cgcatctggt cggcgagttg aatttcgcgg
     4381 gcgccaagct ggcgcgcgaa atctgcgacg cgcgcacgac cgcggaccgg ccgcggctgg
     4441 tggcgggcgt tctcggcccc accaaccgca ccgcgtcgat ttcgccggat gtcaacgatc
     4501 cggctttccg caacatcacc ttcgatgcgt tgcgccgcac ctaccgcgaa gcggcgcttg
     4561 gcctgatcaa gggcggcgcc gacctcttga tgatcgagac gatcttcgac acgctgaaca
     4621 gcaaggcggc gatcttcgcc atcgaggaag ccttcgagga gaccggcgag cgcctgccgg
     4681 tgtggatctc cggcaccatc accgatctga gcggccgcac gctgacgggc cagaccgtcg
     4741 aagccttctg gcattcggtg cgccacgccc agccttttgc cgccggcctc aattgtgcgc
     4801 tgggcgccaa ggagcttcgc cagtatgtgg ccgagctgtc cgccgtggcc gatacgctgg
     4861 tcagcaccca tcccaacgcc ggcctgccca acgaattcgg cggctatgac gataccccgg
     4921 aagagatggc cgagaagatc ggcgagttcg cgcgctccgg gctggtcaat atcgtcggcg
     4981 gctgctgcgg caccacgccc gcccatatca aggcctttgc cgaagcgatc gacggcgtgc
     5041 cgccgcgtgc cgtgcccgaa aagccgcggt acc
//
LOCUS       DASWPU010000446         6697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_65537,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000446 DASWPU010000000
VERSION     DASWPU010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65537"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VGC27_09530"
     CDS             <1..324
                     /locus_tag="VGC27_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282850.1"
                     /translation="NELGLNATYTLDQLSNKYFSAREANGKPITSCEIAGPAPEVNQY
                     LPESWVKWLLAHVLVQDRDFGSANYMPLADGAVYRVVITQTGFNAEPVNDAAAHANSA
                     RNNGI"
     gene            complement(422..2584)
                     /gene="dcp"
                     /locus_tag="VGC27_09535"
     CDS             complement(422..2584)
                     /gene="dcp"
                     /locus_tag="VGC27_09535"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: protein motif:HMM:NF007624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HEY0282851.1"
                     /translation="MPRIRIFGLSAAAAVLLAACSMFDKPVPPPVAPPAETNPFFEPS
                     PLPFQAPQFDRIKDSDFQPAIEEGMKRQLAEIEEIAGNPAPPTFDNTIVAMEKTGQML
                     TRVRLVFSAMTGSSAANTNETLQKVQEIVAPKLAAHRDAIYLNAKLFQRVEAIYAQRD
                     KLDLALEALKLVEVYHHDFVQAGANLPDADKQKLRDINQQISTLETAFTAKLLAGAKA
                     GALVVDAASKLAGLSDGDIAAAAEAAEARGLKGKWVIPLQNTTQQPSLQSLRDRAVRK
                     ELFDRSWTRTERGDENDTRDTISTIAKLRAEKAQLLGFPDFAAYTLEDQMAKTPAAVE
                     KFLSQMVPAATAKARGEVRVIQAVINKSGKRFKLAPWDWNHYAEMVRKAKYDLNEDQI
                     KPYFELNNVLENGVLYAATQLYGITFKERKDIPVYQPEVRVFEVIDKDGSTVGLMYFD
                     YFKRDNKSGGAWMDNFVGQSKLLGLKPVIYNVANFTKPAPGQPALLSFDDVTTMFHEF
                     GHALHGLFADQTYPTLSGTNVARDFVEFPSQFNEHWALDPAVFKRYAVNVETGKPMPQ
                     ALVNKIKKAAKFNQGYMFTELLAAAQLDMQWHTLTAGAPKQDVDEFEIQALKNTHLDL
                     AEVPPRYRSSYFLHIWANGYASGYYAYLWAEMLDDDAFAWFKEHGGLTRENGQRFRDM
                     ILSRGHTLDYGEMYRAFTGRDPQIGPMLEQRGLKTGKN"
     gene            complement(2597..2992)
                     /locus_tag="VGC27_09540"
     CDS             complement(2597..2992)
                     /locus_tag="VGC27_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0282852.1"
                     /translation="MTNGIVKETIRINTKGQAIVPVEIREKAGLLPHTDVEFAFDGHH
                     VRIVRKPGRTKESRGARDGASARAAIAIAPVFPSLLSSRPNETGGGRSMPGIGSPPSP
                     FLEDRRAPRRVRSRSAVRLARRSRPSPQP"
     gene            complement(3036..3554)
                     /locus_tag="VGC27_09545"
     CDS             complement(3036..3554)
                     /locus_tag="VGC27_09545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0282853.1"
                     /translation="MAIAGPEPLTATHDVSQFSCGKSALDNWLKTRALTNQEKGFTAV
                     LVVHESYRVIGYYGLAPTAITPTAMPRSIRTGQPPDPVPCLLLAQLATDLNWTGKGIG
                     TGLLKHALMRCVAAAHLVGGRALIVKAVDAEAVDFWHRRGFIASKDDPLVLFRSMADV
                     AASVRLSAEQTR"
     gene            complement(3566..3862)
                     /locus_tag="VGC27_09550"
     CDS             complement(3566..3862)
                     /locus_tag="VGC27_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEY0282854.1"
                     /translation="MSRVTGRKEHPLSMRLPDSDIAIIDRAAGLRGRSRTDFVRDAAV
                     RAAEDVLMENALIRMSPAGFQNFVAAISGQTVPVPEMVKVLKRRAPWEEAGSRK"
     gene            complement(3955..4815)
                     /gene="purU"
                     /locus_tag="VGC27_09555"
     CDS             complement(3955..4815)
                     /gene="purU"
                     /locus_tag="VGC27_09555"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEY0282855.1"
                     /translation="MSEHADRFILTLSCKDRMGIVADVSHFLFEHRCNIVDSAQFGDP
                     DNGLFFMRTSFQAQAPLAPSRLEADFRPIREKFGMNAALHVQGAKLPTLVLVSKFGHC
                     LNDLLYRHRIGALPVDIRAIVSNHPDFTDVAATAKIPFHFLPVTAATKAAQEAALLAV
                     AREQKVELIVLARYMQVLSPELCAEYSGRIINIHHSFLPSFKGARPYQQAHERGVKLI
                     GATAHYVTGNLDEGPIIEQAVERVTHSFSVEDMVAAGRDLESVVLARAVKWHAEQRIL
                     LNGGRTVIFR"
     gene            complement(4808..>6697)
                     /gene="cysN"
                     /locus_tag="VGC27_09560"
     CDS             complement(4808..>6697)
                     /gene="cysN"
                     /locus_tag="VGC27_09560"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEY0282856.1"
                     /translation="SAMTPTNLKSYLAAQEKKSLLRFLTCGSVDDGKSTLIGRLLYDS
                     KLLFEDHLAALEKDSKKHGTDGDNIDFALLLDGLEAEREQGITIDVAYRFFATDKRKF
                     IVADTPGHEQYTRNMATGASNSELAILLVDARKGVLTQTRRHATIASLLGIRHAVLAV
                     NKIDLADYSQEVFDRIAAEFSEFAAHLGFLSRFAIPISARFGDNVISKSAKTPWYGGP
                     PLLTFLETVDVESALADKPFRLPVQWVNRPNLDFRGFSGTIAGGRVKPGDRIAVAKSG
                     RTSSVKRIVTMDGDLKEAVAGEAVTLTLADEIDISRGDVLCAANDRAEVTDQFAAHLL
                     WMAEEELLPGRQYLLKLATSTVPAQVTELKHKIDVNTLDRLAAKTLRLNEIGTANFSL
                     AQPIAFDSYAANRDMGGFILIDRFSNATVGAGMIDFGLRRATNVHWQALDVNKSARAT
                     LKHQKPAVLWFTGLSGSGKSTIANLVEGALLAQGRHTYLLDGDNVRHGLNRDLGFTDA
                     DRVENIRRVAETAKLFVDAGLIVLVSFISPFRSERRMAREMVGEGEFVEIFVDTPLEV
                     CMARDPKGLYEKAKMGAIRNFTGIDSPYEPPEEAELVVNTVDPDPSVQAARILSYLKE
                     RGYIV"
BASE COUNT         1231 a         2042 c         2135 g         1289 t
ORIGIN      
        1 aacgaactgg gactgaacgc gacctacacg ctcgatcagc tctccaacaa atacttctcg
       61 gcgcgggagg cgaacggcaa gccgatcacc tcttgcgaga tcgcgggacc ggcgccggag
      121 gtcaatcagt atctgcccga aagctgggtc aaatggctgc tcgcccacgt cttggtgcag
      181 gaccgcgact tcggctcggc gaattacatg ccgctggcgg acggcgcggt ttacagggtc
      241 gtgatcaccc agacgggctt caacgccgaa ccggtcaacg acgcggccgc gcacgccaat
      301 agcgcaagaa acaacggaat ctgaggacgg cacccctcat gcttcgacta gctcagcatg
      361 aggggaactg gttacagccc tcaccctgag cctgccgaag ggtgaggatg gcgaaccccg
      421 ttcagttctt ccccgtcttc aacccgcgct gttcgagcat cggcccgatc tgcggatcgc
      481 ggccggtgaa ggcgcgatac atctcgccgt aatccagcgt atggccgcgc gacaggatca
      541 tgtcgcggaa gcgctggccg ttctcgcggg tcaatccgcc atgctccttg aaccaagcga
      601 aggcgtcgtc gtccagcatt tcggcccaca gataggcgta ataaccggag gcgtagccgt
      661 tggcccagat gtgcaggaaa tagctggaac ggtagcgcgg cgggacttcg gccaggtcca
      721 gatgcgtgtt cttcagcgcc tggatttcaa attcgtcgac atcctgcttg ggcgcgccgg
      781 ccgtcagcgt gtgccactgc atgtcgagct gcgccgcggc cagcagttcg gtgaacatgt
      841 agccctgatt gaacttggcg gccttcttga tcttgttcac cagcgcctgc ggcatcggct
      901 tgccggtttc gacgttgacc gcatagcgct tgaacaccgc cggatcgagc gcccaatgtt
      961 cgttgaactg cgagggaaac tccacgaaat cgcgcgcgac gttggtgccg gagagcgtcg
     1021 gatatgtctg atcggcaaac aggccgtgca gggcatggcc gaactcgtgg aacatggtgg
     1081 tgacgtcgtc gaacgacagc agcgcgggtt gccccggcgc gggcttggtg aaattggcga
     1141 cgttgtagat caccggcttc aagccgagca gcttggattg gccgacgaaa ttatccatcc
     1201 aggcgccgcc ggatttgttg tcgcgcttga aatagtcgaa atacatcagg cccacggttg
     1261 agccgtcctt gtcgatcacc tcgaacacgc gcacctccgg ctgatagacc gggatgtcct
     1321 tgcgctcctt gaaggtgatc ccgtaaagct gggtcgcggc ataaagcacg ccgttttcga
     1381 gcacgttgtt gagttcgaaa tacggcttga tctggtcttc gttgagatcg tacttggcct
     1441 tgcgcaccat ctccgcgtaa tggttccagt cccagggcgc gagcttgaag cgcttgccgc
     1501 tcttgttaat gacggcctgg atgacgcgga cctcgccgcg cgccttggcg gtggcggccg
     1561 gcaccatttg ggaaaggaat ttctccaccg cggccggcgt cttggccatc tggtcttcca
     1621 gcgtataggc ggcgaaatcg gggaagccca gcaactgggc cttctcggcg cgaagcttgg
     1681 cgatggtgga aatcgtgtcg cgggtgtcgt tttcgtcgcc ccgctcggtg cgggtccagg
     1741 aacgatcgaa cagctccttg cgcacggcgc gatcgcgcag cgactgcaac gaaggctgct
     1801 gcgtggtgtt ctgcagcggg attacccatt tgcccttgag gccgcgggct tccgccgcct
     1861 cggccgcagc ggcgatatcg ccgtcgctca gacccgccag cttcgacgcg gcatccacca
     1921 ccaaggcgcc ggccttggct ccggcgagca gcttggcggt gaaggcggtt tcgagcgtgg
     1981 aaatctgctg gttgatgtcg cgcagctttt gcttgtcggc gtccggcaga ttggcgccgg
     2041 cctgcacgaa atcgtggtga tacacctcga ccagcttcaa cgcttcgagg gccagatcga
     2101 gcttgtcgcg ctgggcgtaa atcgcctcca cgcgctggaa cagtttggcg ttgaggtaga
     2161 tggcgtcgcg atgggcggcg agcttgggcg cgacgatctc ctgcaccttt tgcagcgtct
     2221 cgttggtgtt ggcggcgctg gagccggtca tcgcactgaa caccagccgc acgcgggtga
     2281 gcatctggcc ggttttctcc atcgccacga tcgtattgtc gaaggtcggc ggcgcggggt
     2341 tgccggcgat ctcttcgatc tcggccagct ggcgcttcat gccctcttcg atggcgggct
     2401 ggaagtcgct atccttgatg cggtcgaact gcggcgcctg gaacggcagc gggctgggct
     2461 cgaagaaggg attggtttcg gccggcggcg ccaccggcgg cggcacgggc ttgtcgaaca
     2521 tggaacaggc ggccagcagc acggcggccg ccgcgctcaa accgaatatg cgaatccgag
     2581 gcatcgcgga atcccctcaa ggttgcggcg acggccgcga gcggcgcgcc agtcgaacgg
     2641 ctgaccgcga ccttactcgg cgcggcgcgc gtctgtcttc aagaaaaggt gaagggggcg
     2701 aaccgatacc cggcatcgat ctgccgcccc ctgtttcatt cgggcgcgat gagagcaagc
     2761 ttgggaaaac aggtgcgata gcgatcgccg cgcgcgcaga tgcgccgtca cgcgcgccac
     2821 gactttcttt cgtccgtccc ggcttgcgca caatccgcac atgatgtccg tcgaaggcga
     2881 actccacatc ggtgtgcggc agcaaaccgg ccttctcgcg gatttctacc ggaacaattg
     2941 cctgcccttt agtattgatg cgtatggtct ctttcacgat tccgtttgtc atgaaaatta
     3001 cggcagctcc attaggatca cggagacgat agcattcagc gtgtttgctc ggcgctcagt
     3061 ctaaccgatg ccgcaacgtc cgccatcgac cggaacagta ccaatgggtc atctttggag
     3121 gcaatgaaac cacgtcggtg ccagaaatct acggcctcgg catcgaccgc cttgacgatc
     3181 agggcgcgcc caccgacgag gtgggcggca gcgacgcaac gcataagcgc atgcttgagc
     3241 aaccccgtgc caatgccttt accggtccag ttcaggtccg ttgcaagttg tgccaacaac
     3301 agacaaggca cagggtccgg cggctgacca gtgcgaatgg aacgtggcat tgcggtcggc
     3361 gtgattgcgg tcggcgccaa gccataatat ccgatgacgc gatagctctc atgcacaaca
     3421 aggacagccg tgaacccttt ttcctgattt gtcagcgcgc gtgtctttag ccagttgtcg
     3481 agggcagact tgccgcatga gaattgcgaa acgtcatgcg tcgccgtcaa aggttcgggg
     3541 cccgcaatag ccaagatgaa ccccgctatt tccttgaacc agcttcttcc cacggcgccc
     3601 ggcgcttgag cactttcacc atctcaggaa ccggcactgt ctgacctgaa atcgcggcca
     3661 caaaattctg aaatcctgcg gggctcatgc gaataagcgc attttccatg agcacgtctt
     3721 cggcggcacg gacggcggcg tcccggacga aatccgtccg cgagcgccct cgcaaccccg
     3781 ccgcgcggtc gatgatggca atatcggagt cgggcagccg catcgacaac ggatgttctt
     3841 ttcttccggt gacacgagac atttcacaaa tcctgcttat acacaggtaa gatagcactc
     3901 tgtagtgctt tttgcaatac aaaggccgca cactgataag cctcgacatg cctgctaccg
     3961 gaaaatcacc gtccgcccgc cgttgagcag gattcgctgc tcggcgtgcc atttgaccgc
     4021 ccgcgccagc acgacggatt cgaggtcgcg gcccgccgcc accatatcct ccaccgagaa
     4081 gctgtgcgtc acgcgctcca ccgcctgttc gatgatcggc ccctcgtcga gattgccggt
     4141 cacgtaatgc gccgtggcgc cgatcagttt gacgccgcgc tcgtgcgcct gctggtaagg
     4201 cctggcgccc ttgaaactgg gcaggaagga atggtggatg ttgatgatcc ggcccgaata
     4261 ttccgcgcaa agttccggcg acagcacctg catgtagcgc gccagcacga tcaactcgac
     4321 cttttgctcc cgcgcgacgg cgagcagcgc ggcttcctgg gccgctttgg tggccgccgt
     4381 gaccggcagg aaatggaacg gaattttcgc cgtcgccgcc acgtcggtga aatcgggatg
     4441 gttggagacg atggcgcgga tgtccaccgg caaggcgccg atccggtggc gatacaaaag
     4501 atcgttcagg caatggccga atttggagac cagcaccaga gtgggcagtt tcgcgccctg
     4561 gacatgcagc gcggcgttca tgccgaattt ctcgcggatg ggccggaaat ccgcttcgag
     4621 ccgcgacggc gccagcggcg cctgcgcctg gaagctggtg cgcatgaaga acaggccgtt
     4681 gtccggatcg ccgaactggg cgctgtccac gatgttgcag cgatgttcga acaggaaatg
     4741 cgagacgtcc gccacgatac ccatccggtc cttgcaggac agggtgagga tgaaacggtc
     4801 ggcgtgctca gacaatgtag ccccgctcct tgaggtagct gagaatcctc gcggcttgca
     4861 ccgacggatc cggatcgacg gtgtttacca ccagttcggc ctcttcggga ggctcatagg
     4921 ggctgtcgat gccggtgaag ttcctgatcg cgcccatctt ggccttttcg tacaggcctt
     4981 tgggatcgcg cgccatgcag acctcaagcg gcgtatcgac gaagatttcc acgaactcgc
     5041 cctcgcccac catctcgcgc gccatgcgcc gttccgagcg gaacggcgag atgaacgaca
     5101 ccagcacgat caacccggcg tcgacgaaca gcttggcggt ttcggccaca cggcggatgt
     5161 tctccacgcg gtcggcatcg gtgaagccga ggtcgcggtt gaggccgtga cgcacattgt
     5221 cgccgtcgag cagataggta tggcggcctt gcgcgagcag cgccccctcc accagattgg
     5281 cgatggtcga tttgcccgat cccgacaggc cggtgaacca cagcacggcc ggcttctggt
     5341 gcttgagcgt ggcgcgcgcg gacttgttca cgtcgagcgc ctgccaatgc acattggtgg
     5401 cgcgcctgag cccgaaatcg atcatcccgg cgccgaccgt ggcgttgctg aagcggtcga
     5461 tcaggataaa gccgcccatg tcgcggttgg ccgcatagga atcgaaggca atcggctggg
     5521 cgagcgagaa attggcggtg ccgatctcgt tcagacgcag ggtcttggcc gccagccggt
     5581 cgagcgtgtt gacgtcgatc ttgtgcttga gttcggtgac ctgcgccggg accgtcgagg
     5641 tggcgagctt cagcagatat tgccggccgg ggagcagttc ctcttccgcc atccacagaa
     5701 gatgcgccgc gaattgatcg gtaacctcgg cgcggtcgtt cgccgcgcac agcacgtcgc
     5761 cgcgcgagat gtcgatctcg tcggcgaggg tcaacgtgac cgcctcgccg gccacggctt
     5821 ccttcaggtc gccgtccatg gtgacgatgc gcttgaccga gctggtgcgc ccggacttcg
     5881 ccacggcgat cctatcgccc ggcttgacgc ggccgccggc gatcgtgccg gaaaagccgc
     5941 ggaaatcgag attggggcgg ttcacccact gcaccggcag gcggaacggc ttatccgcga
     6001 gcgcggactc cacatccacg gtctcgagga aggtcagcag cggcgggccg ccgtaccagg
     6061 gcgtcttcgc gctcttcgag atcacattgt cgccgaagcg cgccgaaatc gggatcgcga
     6121 agcggctgag gaagcccaga tgcgcggcga attcggagaa ctccgcggcg atgcggtcga
     6181 acacctcctg cgaataatcg gccagatcga tcttgttcac cgccagcacg gcgtggcgga
     6241 tgccgagcag cgaggcgatg gtggcgtggc ggcgggtctg ggtcagcacg cccttgcgcg
     6301 catcgaccag caggatggcc agttcggaat tcgaggcgcc ggtcgccatg ttgcgggtgt
     6361 attgctcgtg gcccggcgtg tcggcgacga tgaatttgcg cttgtcggtg gcgaagaagc
     6421 gataggcgac gtcgatggtg atgccttgtt cgcgctcggc ttccagcccg tcgagcagga
     6481 gcgcgaaatc gatattgtcg ccgtcggttc cgtgtttctt cgagtccttt tccagcgccg
     6541 ccagatgatc ctcgaacagc agcttggaat cgtagagcag ccggccgatc agcgtggact
     6601 tgccgtcatc gacgctgccg caggtgagaa agcgcagcag cgacttcttc tcctgcgcgg
     6661 cgagatagga tttgaggttg gtgggagtca tcgcact
//
LOCUS       DASWPU010000447         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_83653,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000447 DASWPU010000000
VERSION     DASWPU010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83653"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1700
                     /gene="glnD"
                     /locus_tag="VGC27_09565"
     CDS             <1..1700
                     /gene="glnD"
                     /locus_tag="VGC27_09565"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY0282857.1"
                     /translation="NKKRRAMLASILPGFLKPRGKDDDFFVDTGRLNARPNAFKSNPV
                     NLIRIFHVADAKSVDIHPEALRTITRSLDLIDDALRADPAANRLFLEILSSRSDPERA
                     LRLMNEAGVFGKFMPEFGRVVALMQFNMYHHYTVDEHLIRAIGNVASIERGELDEEHP
                     LAADIIKRIKTREVLYCAMLLHDIAKGLPGDHSDIGAAIANSVCPRLGLSAEDTASVA
                     WLVENHLIMSDMAQRRDVADPKTVRDFVAVVQSPEMLRLLLVLTVADIRAVGPGVWNG
                     WKGQLLRELYLEAETVMSGGDAIRARSARVEEAKEALEKRIADLPEKARERALSRHYD
                     GYWLALDAETHERNARMLAKADASGELLALAAVSNDFRSVSEVTVYTPDHPGLFAQIA
                     GAIAVSNGSIVDAKIFTTTDGFALDVFSVQDAEGGPFGDAARIARLRQTIEKTLTGEI
                     VPRHLIAKRPPKSRASAFRVSQRVNFDNDASTTATVIEVRGPDRVGFLYDVTTALFES
                     GLSISSAIIATYGERVVDVFYVRDGYGHKVTHPERLASIEARLLKALEGENAETAAS"
     gene            1904..2929
                     /gene="trpS"
                     /locus_tag="VGC27_09570"
     CDS             1904..2929
                     /gene="trpS"
                     /locus_tag="VGC27_09570"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY0282858.1"
                     /translation="MSDAPKHKQLVFSGMQPTNTLHLGNYLGALKNWVKLQDRMPCIY
                     CVVDMHAITADAGYSHPGELTAATREVAAAYIAAGVDPKRSILFNQSRVPAHAELAWI
                     FNCVARLGWMDRMTQFKEKSGKHKERASVGLYTYPTLMAADILVYKATHVPVGEDQKQ
                     HLELARDIAQKFNNDFAAPGFFPLPEPVITGAGTRVMSLRDGAKKMSKSDDSDYSRIN
                     LTDDADAIAQKIRKAKTDPAPLPDSEKGLEGRPEAENLFNIYAALAEEPRAQVIGRFA
                     GQQFSGFKNALAELAVAKLTPIAAEMRRLLADPAEIDRVLDDGAARAEALARPVLAEA
                     KRLVGFL"
BASE COUNT          558 a          994 c          926 g          467 t
ORIGIN      
        1 agaacaagaa gcgccgcgcc atgctggcga gcatcctgcc cggcttcctc aagccgcgcg
       61 gcaaggacga cgacttcttc gtggacacgg gacggctgaa cgcccgtccg aacgctttca
      121 agagcaaccc ggtcaatctc atccgcatct tccacgttgc cgacgccaag agcgtcgaca
      181 tccaccccga agcgctgcgc accatcaccc gctcgctcga cctgatcgac gacgcgttgc
      241 gcgccgatcc cgcggccaac aggctgttcc tggagatact ttcctcgcgc agcgatcccg
      301 agcgcgcgct caggctgatg aacgaggcgg gcgtcttcgg caagttcatg ccggagttcg
      361 ggcgcgtcgt cgctctgatg cagttcaaca tgtaccacca ctacacggtg gacgagcatc
      421 tgatccgcgc gatcggcaac gtcgcgtcga tcgagcgcgg cgagttagac gaagaacatc
      481 cgctggccgc cgacatcatc aagcgcatca agacgcgcga ggtgctctat tgcgcgatgc
      541 tgctgcacga catcgccaag ggcctgccgg gcgaccattc cgacatcggc gcggcgatcg
      601 ccaacagcgt ctgcccgcgg ctcggcctgt cggcggagga taccgcctcg gtcgcctggc
      661 tggtggaaaa ccacctgatc atgagcgaca tggcccagcg ccgcgacgtg gccgatccca
      721 agacggtgcg cgatttcgtc gccgtcgtgc agtctcccga gatgctgcgg ctgctgctgg
      781 tgctgacggt ggccgacatc cgcgcggtgg gccctggcgt gtggaacggc tggaagggcc
      841 agcttctgcg cgagctttat ctcgaggccg aaaccgtgat gtcgggcggc gacgcgatac
      901 gggcgcgcag cgcgcgcgtc gaggaagcca aggaagctct ggagaagcgc atcgccgacc
      961 tgccggaaaa agcgcgcgag cgggccttgt cgcgccatta cgacggttat tggctggcgc
     1021 tcgacgccga aacgcacgag cgcaacgccc ggatgctggc caaggcagac gccagcggcg
     1081 agctcttggc gctcgcagcc gtaagcaacg atttccgctc ggtcagcgag gtcaccgtct
     1141 atacgccgga ccatccgggc ctgttcgccc agatcgccgg cgccatcgcg gtctcaaacg
     1201 gctcgatcgt ggacgccaaa atcttcacca ccacggacgg cttcgcgctc gacgtgttct
     1261 cggtgcagga cgcggaaggc ggtccgttcg gcgacgcggc ccgcatcgcg cgcctgcgcc
     1321 agaccatcga gaagacattg accggcgaga tcgtgccgcg ccacttgatc gccaagcgcc
     1381 cgcccaagag ccgcgccagc gccttcaggg tgagccaacg ggtcaatttc gacaacgacg
     1441 cctccaccac cgccaccgtc atagaagtgc gcggccccga ccgggtggga ttcctctacg
     1501 acgtgaccac ggcgctgttc gaatccggcc tgtcgatctc ctcggcgatc atcgccactt
     1561 acggcgagcg cgtggtggac gtgttctatg tgcgcgacgg ctacggccac aaagtcaccc
     1621 accccgagag gctggcgtcg atcgaggcgc ggctgctcaa ggcgctggaa ggtgagaatg
     1681 ctgaaacggc ggcgagctaa ccttgtcatg gcccgcgaat tcgtagcgac agcgtgcgaa
     1741 cgggccaccg gggtgaaacc ggcgccatag ttttcaggaa cgtgcgtgag atcaactggg
     1801 cggcccgcac caggcgggcc gtgacaacga gtttgaatga cacagaacct tgcgacttcg
     1861 gccgcttcgc gtcataaccc ccgcgcctta gccgagggtt tccatgtccg acgcgcccaa
     1921 gcacaagcaa ctcgtctttt ccggcatgca gccgaccaac acgctgcatc tgggcaatta
     1981 tctgggcgcg ctgaagaact gggtgaagct tcaggatcgg atgccgtgca tctattgcgt
     2041 ggtggacatg cacgccatca ccgcggacgc cggctatagc catcccggcg aactgacggc
     2101 ggccacccgc gaagtcgcgg cggcttacat cgccgcgggc gtcgacccca aacgttccat
     2161 ccttttcaac cagagccggg tgcccgcgca tgccgaactc gcctggatct tcaattgcgt
     2221 ggcgcggctg ggctggatgg accgcatgac ccagttcaag gaaaaatccg gcaagcacaa
     2281 ggaacgcgcc tcggtcgggc tctacaccta tccgacgctg atggccgccg acatcctggt
     2341 ctacaaggca acgcatgtgc cggtgggcga ggaccaaaag cagcatctcg aactggcccg
     2401 cgacatcgcg cagaaattca acaacgattt cgccgcgccc ggtttcttcc ccctgcccga
     2461 gccggtgatc acgggcgcgg gcacgcgggt gatgagcctg cgcgacggcg ccaagaaaat
     2521 gtcgaagtcc gacgacagcg attattcgcg catcaacctg accgacgacg ccgacgccat
     2581 cgcccagaaa atccgcaagg ccaagaccga tcccgcgccg ctgcccgatt ccgagaaagg
     2641 gctggagggc cggcccgaag ccgaaaacct gttcaacatc tatgcggcgc tggccgaaga
     2701 gccgcgcgcg caggtgatcg ggcgcttcgc gggccagcaa ttctccggct tcaagaacgc
     2761 cctggcggaa ctggcggtcg ccaagctgac gcccatcgcc gccgaaatgc gccgcctgct
     2821 cgccgatccg gcggaaatcg accgcgttct ggacgacggc gcggcgcgcg ccgaggccct
     2881 cgcccgaccc gttctggcgg aggcgaaacg gctggtgggg tttctttagt tctctttgcc
     2941 tcctc
//
LOCUS       DASWPU010000448         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_22583,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000448 DASWPU010000000
VERSION     DASWPU010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22583"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..1775
                     /locus_tag="VGC27_09575"
     CDS             405..1775
                     /locus_tag="VGC27_09575"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY0282859.1"
                     /translation="MRFSGGAILCATILLTTSAYASGFGLREVSASAFGSSYAGAPAP
                     GGEASTMFFNPALVADVKTVDISSSVLGLLPDATGNFTATTAASTPAGGAAHVKDIVN
                     TALIPSLAVRARLTDRFTVGLQVTAPWGMITDYGPDWVGRYYAAQSDVKTANVTAVAG
                     FQPVPELAFAAGLQVQYIKGRLGKAIDFGTIAAGPPFGLPVPAVTIPGMRDGFVLLKA
                     QNWGLGYVLGLTWTPGERLSLGLSYRSQIDNTLKGTETFDLGSVPVTALGGVTVGQFI
                     AAHTGWFQNGGAQADFSTPASVTFGGRYKITDRLTALASVDWTDWTAFKEVRAVTVPN
                     PYNQPDDVTTMAWKASWYGAVGLEYKNNGWSFRVGTAYDGTPTTDQYRTPGIPDGSRT
                     WLTIGAGVRLTDHMNLDVSYGHLFFQKGNIDLSVATPENRARGSLNGVVNMNVDILGG
                     EVSYHL"
     gene            1902..3011
                     /locus_tag="VGC27_09580"
     CDS             1902..3011
                     /locus_tag="VGC27_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0282860.1"
                     /translation="MRGSDERSGSLFSYVDLEARVRRDHPLRTIREVANAALNDLSGD
                     FGKLYTDFGRPSIAPEKLLRAMLLQAFYGVRSERQLMERLEFDLLFRWFVGLGVDDAV
                     WDHSVFSKNRDRLLEGEIAAKFLNAILTQPRVKKLLSSDHFSVDGTLIEAWASLKSFR
                     RKDGNDKDNDGPGRNAERSFHKEKRSNETHESTTDPEARLYKKGDGQPAKLCYMGHAL
                     MENRNGLAVCGGVSQATGTAEREDALALVDRHRGRSKRRITLGADKAYDVAQFVQDLR
                     QRQVTAHIAIDGHLTKTGKSRKTAIDGRTLRHAGYAISQRCRKRIEEVFGWIKSSAGL
                     RKVKLRGRARVDAVFTMALAAYNLIRLPKLLAAPA"
     gene            3008..>3286
                     /locus_tag="VGC27_09585"
     CDS             3008..>3286
                     /locus_tag="VGC27_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282861.1"
                     /translation="MKGKWRIVELPGYEPDYADMVEPAYILFDGKGGGEFAFGCVSGG
                     IDGAGETDNVVFTWAGSDEMDEVSGDGWAELQPDGSLQGQICVHYGDEI"
BASE COUNT          609 a          992 c         1081 g          604 t
ORIGIN      
        1 gcgtcggcgc cgcccccgaa atcatccgct gggtcgaaga ccggggcgaa gaattgcgtg
       61 tggcgcttgc ccgcaagggc gccgccaatg tcgaattgga agaagaccaa acttgttccc
      121 gcgagagctt cgatgtcgcc accatcgtct gaaagcgggg ggcggaaggc cggaacctgc
      181 cccatctgca agaaaccggc ggccgagcgg tttcgtcctt tctgttcaag gcgctgcgcg
      241 gaactcgatc ttgcccattg gttgcgcgga gattatgcga ttccggcagc ttcccgggat
      301 tctcaagagg atagcgatcc tccggcccaa ttgccgcatg gggattagaa cgtttcatcc
      361 ttctgttata tttgccccta gggagacaaa ctctggggat tctcatgcgc ttttcgggcg
      421 gcgcaattct ttgcgctaca atactgctta cgacgtcggc ttatgcgtcg ggcttcggcc
      481 tgcgcgaagt gagcgcgagt gcttttggca gttcctacgc gggtgcgccc gcgccgggag
      541 gcgaagcctc gaccatgttc ttcaatccgg cgctggtcgc cgacgtcaag acggtcgata
      601 tctcttcgag cgtgcttggc ctgttgccgg atgccacggg aaacttcacc gcgaccaccg
      661 ctgcttcgac accggccggc ggcgcggctc atgtgaaaga cattgtaaat accgcgttga
      721 tcccgtcgct ggcggttcgc gcgcgcctca ccgaccggtt cacggtgggc ctgcaagtca
      781 ccgcgccctg gggcatgatt acggactacg gcccggactg ggtcggccgc tattacgcgg
      841 cgcagagcga cgtgaagacg gcgaacgtca cggcggtggc cggcttccag ccggttccgg
      901 aactcgcctt cgcggcgggc ctccaggtgc agtacatcaa gggcaggctc ggcaaggcga
      961 tcgacttcgg tacgatcgct gccggccctc cgttcgggct gcccgttccg gcggtgacga
     1021 tccccggcat gcgggacggc ttcgtccttc ttaaggccca gaactggggg ctcggctatg
     1081 ttctcggcct gacctggaca cccggcgaga ggctgtcgct tggactttcc tatcgctcgc
     1141 agatcgacaa cacgctgaag ggaacggaaa ccttcgattt gggctcggtg ccggttacgg
     1201 cgctgggcgg cgtcaccgtg ggacagttca tcgccgcgca taccggctgg ttccagaacg
     1261 gcggcgcgca ggccgatttc tcgactccgg cctccgtgac gttcggcggg cgctacaaga
     1321 tcaccgaccg gctcaccgcg ctggccagcg ttgattggac ggactggacc gccttcaagg
     1381 aagtgcgggc cgtgaccgtg cccaacccgt acaaccagcc cgacgacgtc acgacgatgg
     1441 cctggaaagc ctcctggtac ggcgctgttg gcctcgagta caagaataac ggctggtcgt
     1501 tccgggtcgg tacggcttat gacgggactc cgaccaccga ccagtatcgc acccccggca
     1561 ttcccgacgg cagccgcact tggctgacga tcggcgccgg cgtgcggctg accgaccaca
     1621 tgaatctcga cgtgtcttac ggccacctgt tcttccagaa gggcaacatc gatctgtccg
     1681 ttgcgacgcc ggagaaccgt gcccgcggct ctttgaacgg cgtggtgaac atgaacgtgg
     1741 acattctggg tggagaggtc agctaccacc tgtaaacgga tcgccggttc ggaccgggaa
     1801 taatgcactg ctcaccgcgg ctagcaggtt gttgaaatag tccctggcgc ggctgtgatg
     1861 atcgtgattc cctggatgtg gaagtgcgcc ggggatgatg gatgcgcggg agcgacgagc
     1921 ggtctggatc actgttcagc tatgtggatt tggaggcgcg ggttcggcgc gatcatccgc
     1981 tacgaacgat ccgggaggtt gcgaatgcgg ctctgaacga tttgtccggc gattttggca
     2041 agctctacac ggattttggg cgcccttcga tcgcaccaga gaagctgctg cgggcgatgc
     2101 tgttgcaggc gttctacggg gtgcgctcgg aacggcaatt gatggagcgg ctggagttcg
     2161 accttttgtt ccgctggttc gtggggcttg gcgtggacga cgcggtgtgg gaccactcgg
     2221 tgttctcgaa gaaccgcgac cggctgctgg aaggcgagat tgccgccaag ttcctgaacg
     2281 cgatcctgac ccagcctcgg gtgaagaagc tgttgtcaag cgatcacttc tcggtggacg
     2341 ggacactgat cgaggcgtgg gcatcgctca agagcttccg gcgcaaggat ggcaacgaca
     2401 aggataatga cggtcccggc cgcaacgccg agcgcagctt ccacaaggag aagcgctcga
     2461 acgaaaccca tgagagcacg accgatcccg aggcgcggct ttacaagaag ggcgacggcc
     2521 agccagccaa gctctgctac atgggccacg ccttgatgga gaaccgcaac ggactggcgg
     2581 tgtgtggcgg ggtgagccag gcaaccggca cagccgagcg cgaggacgcc ttggcgttgg
     2641 tcgaccgcca tcggggtcgg agcaaacgtc gaatcacgct gggagccgac aaagcctatg
     2701 acgtcgccca gttcgtacag gacctgcggc aacgacaggt caccgcgcac atcgccatcg
     2761 acgggcatct gaccaagacc ggcaagtcgc gcaagacggc gatcgatggg cgcacgctgc
     2821 gccacgccgg ttacgccatc agccagcgct gccgcaagcg catcgaggag gtgttcggct
     2881 ggatcaagag ttccgcagga ctacgaaagg tcaagctgcg aggacgcgcc cgggtggacg
     2941 ccgtcttcac catggcgcta gcggcttaca atctgatccg cctgcccaag ctgttggcag
     3001 cccccgcttg aagggcaaat ggcgcattgt cgaactaccc ggctacgagc ccgactatgc
     3061 cgacatggtc gagcccgcct atatcctgtt cgacggcaag ggtggcggcg agttcgcctt
     3121 cggctgcgtc agcggcggca tcgacggagc cggggaaacc gacaatgtcg tgttcacctg
     3181 ggcaggaagt gacgaaatgg acgaggtcag cggcgatggc tgggccgaac ttcagcccga
     3241 cggctccctc cagggtcaaa tctgcgtcca ctacggcgac gaaatc
//
LOCUS       DASWPU010000449         7517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_58852,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000449 DASWPU010000000
VERSION     DASWPU010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58852"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VGC27_09590"
     CDS             complement(<1..481)
                     /locus_tag="VGC27_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0282862.1"
                     /translation="MTRKLVIDGKEVEADDNLTLLQACEQAGAEVPRFCYHERLSVAG
                     NCRMCLVEWVGAPKPQASCSLQVKDVFPNKDGTPAKINTNSPYVKKARQGVMEFLLIN
                     HPLDCPICDQGGECDLQDQAMAYGFDRGRYHEMKRAVKDKDLGPLVETCMTRCIHCTR
                     "
     gene            complement(633..1070)
                     /locus_tag="VGC27_09595"
     CDS             complement(633..1070)
                     /locus_tag="VGC27_09595"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0282863.1"
                     /translation="MKKIIAASVVLISLAVSAQADPAANKKLVLDMADMLFVQHKVDQ
                     AIDTYFAPGYIQHNPMAADGSEAIRTFFKGFYAGNPQATIEVKRALADGDLVAIHYKA
                     KFTPDERGYAVVDIFRIENGKIAEHWDAVQPIPEKSANTNGMF"
     gene            complement(1080..2363)
                     /gene="nuoF"
                     /locus_tag="VGC27_09600"
     CDS             complement(1080..2363)
                     /gene="nuoF"
                     /locus_tag="VGC27_09600"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY0282864.1"
                     /translation="MLADKDRIFTNLYGFGDPGLKGARARGNWDGTKKILELGPEAIV
                     DTIKKSGLRGRGGAGFPTGLKWSFMPKEIKDRPHYLIVNADESEPGTCKDRDILRHDP
                     HTLVEGCLIASFAMRAHAAYIYVRGEFIREREALQRAVDEAYDAGLIGKDNIHGWDFD
                     IYVAHGAGAYICGEETALLESLEGKKGQPRMKPPFPANVGLYGCPSTVNNVESIAVAP
                     AIMRRGAEWFAGIGRPNNTGTKVFCISGHVNTPCNVEEAMGISLRELIETHAGGVRGG
                     WDNLLAVIPGGASVPLLPKSVCDDVLMDFDSLKNVQSGLGTAAVIVMDKSTDVVQAIA
                     RLSQFYKHESCGQCTPCREGTGWMWRVMTRLQRGEARAEEIDLLLEVTKEVEGHTICA
                     LGDAAAWPIQGLIRHFRPELERRIAQRNATAKAAE"
     gene            complement(2490..3092)
                     /gene="nuoE"
                     /locus_tag="VGC27_09605"
     CDS             complement(2490..3092)
                     /gene="nuoE"
                     /locus_tag="VGC27_09605"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005724.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY0282865.1"
                     /translation="MSLRRLHHEQPASFAFTAANKARVDKEIAKYPAGRQASAVIATL
                     WIGQEQEGWVTKPMIEAVATLLDMPFIRVLEVATFYTMFNLKPVGEHLVQVCTTTPCW
                     LRGSDEVVKACKKHIHEKPHTVSADGKFSWEEVECLGACVNAPMIQIDGDTYEDLDGP
                     ATERILQALRDGKTPKPGSQTGRHSSEPAGGPTALKEVQS"
     gene            complement(3089..4264)
                     /locus_tag="VGC27_09610"
     CDS             complement(3089..4264)
                     /locus_tag="VGC27_09610"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY0282866.1"
                     /translation="MSDVDGKITINFGPQHPAAHGVLRLVMELDGEIVSRIDPHIGLL
                     HRGTEKLIEHKTYLQAIPYFDRLDYVAPMNQEHAFCLAIEKLLGLEVPRRGQYIRVLY
                     AEIGRLLSHLLNVTTQANDVGALTPILWGFEEREKLMVFYERASGSRLHAAYFRPGGV
                     HQDLPPELLEDIYTFCGTFPKVLDDIETLLTDNRIFKQRNVDIAVFNREEAEARGFSG
                     VMLRSTGVAWDLRRAQPYECYSDLDFQIPVGKNGDCYDRYVMRMEEMRQSTKIMKQCI
                     EKMPSGPSVSTDNKIVPPRRAEMKTSMEALIHHFKLYTEGFHVPAGEVYAAVEAPKGE
                     FGVYLVADGTNKPYRCKIRAPGFPHLQALEYLCKGHMLADVSAVIGSIDIVFGEIDR"
     gene            complement(4517..5119)
                     /locus_tag="VGC27_09615"
     CDS             complement(4517..5119)
                     /locus_tag="VGC27_09615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004733.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY0282867.1"
                     /translation="MTALTVLTDLATKIADALPGAIKASKLAFGELTLDAEAGEIVRV
                     LTFLRDQCEFKVLIDICGADWPAREKRFDVVYHLLSLTENRRIRVKVETGEAAPVPSA
                     IPVFPAADWYERETFDLYGVVFEGHPDLRRIITDYDFEGHPLRKDFPLTGFVELRYDD
                     DQKRVVYQPVQLVQEYRDFDFMSPWEGMPQVLPGDEKAGK"
     gene            complement(5116..5643)
                     /locus_tag="VGC27_09620"
     CDS             complement(5116..5643)
                     /locus_tag="VGC27_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY0282868.1"
                     /translation="MGVIPAPSRTDNDPYFQAMQDELADKGFLVAGADALINWARTGS
                     LMWMTFGLACCAIEMMQASMPRYDLERFGFAPRASPRQSDVMIVAGTLCNKMAPALRK
                     VYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPSAEALVYGI
                     MLLQRKIRRTGTIER"
     gene            complement(5643..5999)
                     /locus_tag="VGC27_09625"
     CDS             complement(5643..5999)
                     /locus_tag="VGC27_09625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY0282869.1"
                     /translation="MERLLLEYLPILIFIGIAGVIALGLAVVPLVIAPAKPDPEKLSA
                     YECGFEAFGDARMKFDVRFYLVSILFIIFDLEVAFLFPWAITLGDTGVFAFWSMMAFL
                     GVLTVGFIYEWKKGAL"
     gene            complement(6999..>7517)
                     /locus_tag="VGC27_09630"
     CDS             complement(6999..>7517)
                     /locus_tag="VGC27_09630"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0282870.1"
                     /translation="AFTLMEPGPVILVTTNDGRKDNVMTISWTMVLDFSASFAITTGP
                     WNYSYAALRKSKACVISLPTVDLMDTVVGVGTCSGKDTDKFEKFGLTRVKAKHVRAPL
                     IKECLANIECRVVDVVDRYSIIVLEGVAAYFDGSRKEKRTIHAVGDGTFVTDGRRIDR
                     KKMMRSKLPGGL"
BASE COUNT         1416 a         2368 c         2286 g         1447 t
ORIGIN      
        1 agcgcgtgca gtggatgcag cgggtcatgc aggtctcgac cagggggccg aggtccttgt
       61 ccttcaccgc gcgcttcatc tcgtgatagc ggccgcggtc gaacccgtag gccatcgcct
      121 gatcttgcag gtcgcattcg ccgccctggt cgcagatcgg gcaatcgagc ggatggttga
      181 tgagcaggaa ctccatcacg ccctggcgcg ccttcttcac gtaaggcgaa ttggtattga
      241 tcttggcggg cgtgccgtcc ttgttgggga agacgtcctt cacttgcagc gaacaggagg
      301 cttgcggctt gggcgcgccg acccactcca ccaaacacat ccggcaattg cccgccaccg
      361 acagccgctc gtgatagcag aaacgcggaa cctcggcgcc ggcctgctcg caggcttgca
      421 gcagcgtgag gttgtcgtcc gcttccactt ccttgccgtc gatgacgagc ttacgggtca
      481 cgaccgacgc tccaaaacaa tactgtcatg gccgggcttg acccggccat ccagcggtgg
      541 cgtgtccacg tcgccaatga gtccttcacc atctggatgg cccgcttttg cgggccatga
      601 caagaagggt ctgatgacga gcttacgggg catcagaaca tgccgttcgt gttggccgac
      661 ttttcgggaa tgggctgaac cgcgtcccaa tgttcggcga tcttgccgtt ttcaatgcgg
      721 aagatgtcca cgaccgcata accgcgctcg tcgggggtga atttcgcttt gtagtggatg
      781 gccacaagat cgccgtccgc cagcgcgcgc ttgacttcga tcgtcgcctg gggatttccg
      841 gcatagaagc ccttgaagaa ggtgcggatg gcctcgctgc cgtccgcggc catcggattg
      901 tgctggatgt agccgggcgc gaaataggtg tcgatcgcct gatcgacttt gtgctggacg
      961 aacagcatgt ccgccatgtc gagcacgagc ttcttgttgg cggccggatc ggcttgcgcc
     1021 gaaaccgcga ggctgatcaa cacgaccgat gctgcaataa tttttttcat ttgcgtctcc
     1081 tattctgccg cttttgccgt ggcgttgcgt tgcgcgatgc gccgttcgag ttcggggcgg
     1141 aaatggcgga tcaggccctg gatcggccag gccgccgcgt cgccgagcgc gcagatggtg
     1201 tggccttcca cttccttggt cacttccaga agcaggtcga tttcctccgc gcgggcctcg
     1261 cccctttgca ggcgcgtcat cacccgccac atccagccgg tgccttcgcg gcagggcgtg
     1321 cactggccgc agctttcgtg cttgtagaat tgagagagcc gcgcaatcgc ctgcaccacg
     1381 tcggtggatt tgtccatcac gatcaccgcg gcggtgccga ggcccgattg cacgttcttc
     1441 agcgagtcga aatccatcag cacgtcgtcg cagaccgatt tggggagcag cggcaccgag
     1501 gcgccgccgg gaataaccgc aagcagattg tcccagccgc cgcgcacccc gccggcatgc
     1561 gtttcgatca gctcgcgcaa cgagatgccc atcgcctctt cgacgttgca cggtgtgttc
     1621 acatggccgg agatgcagaa caccttggtg ccggtgttgt tcggccgccc gatgccggcg
     1681 aaccattcgg cgccgcgccg catgatggcc ggcgccaccg cgatgctttc gacattgttg
     1741 accgtcgagg ggcagccata aagcccgaca ttggccggga acggcggctt catgcgcggc
     1801 tggcccttct tgccttccag gctttcgagc agcgcggtct cctcgccgca gatgtaagcg
     1861 cccgcgccgt gcgcgacgta aatatcgaag tcccagccgt ggatattgtc cttgccgatc
     1921 agccccgcgt cataggcttc gtccactgca cgctgcaacg cctcgcgctc gcggatgaac
     1981 tcgccgcgca catagatgta ggccgcgtgc gcccgcatcg cgaagctcgc gatcaggcag
     2041 ccttcgacca gcgtatgcgg atcgtggcgc aggatgtcgc ggtccttaca ggtgccgggc
     2101 tcggattcgt cggcgttgac gatcagataa tgcggccggt ccttgatttc cttcggcatg
     2161 aacgaccatt tgaggccggt cgggaagccc gcgccgccgc gcccgcgcaa gccggatttc
     2221 ttgatcgtat cgacgatggc ttcgggaccg agttcgagaa tcttcttggt gccgtcccaa
     2281 ttgccgcgcg cgcgcgcacc cttaaggccc ggatcgccga agccgtagag attggtgaag
     2341 atgcggtcct tatcggcgag catcgaacaa gctcccgcca ccaagattgt cacgcggaga
     2401 cgcagagaac gcggagtgca acagttgctc cgcgacctcc gcgcctccgc gtgccatttc
     2461 cccgccagat cttgacgaaa gaagagcgat cacgactgca cctccttcaa cgccgtcggc
     2521 ccgccggccg gctcggagga atggcggccg gtctgcgaac cgggcttggg cgttttgccg
     2581 tcgcgcaacg cttgcagaat tctttcggtc gccgggccgt cgaggtcttc gtaagtgtcg
     2641 ccgtcgatct ggatcatcgg cgcgttgacg caagcgccga ggcattccac ctcttcccag
     2701 gagaattttc cgtcggccga aaccgtgtgc ggcttttcgt ggatatgctt cttgcaggct
     2761 ttgaccactt catcggaacc gcgcagccag cagggcgtgg tggtgcagac ctgcaccaga
     2821 tgctcgccca ccggtttcag gttgaacatg gtgtagaagg tggcgacttc cagcacgcgg
     2881 atgaacggca tgtcgagcag tgttgccacc gcctcgatca tcggcttggt gacccagcct
     2941 tcctgttcct ggccgatcca cagcgtggcg atcaccgccg aggcctgacg cccggcggga
     3001 tatttcgcga tctccttgtc cacgcgcgcc ttgttcgccg ccgtgaaagc gaagctcgca
     3061 ggctgttcgt gatgcaggcg gcgaaggctc aacggtcgat ctccccgaac acgatgtcga
     3121 tcgagccgat caccgcggac acgtcggcca gcatgtggcc tttgcagagg tactccagcg
     3181 cttgcagatg cggaaacccc ggcgcacgga ttttgcagcg gtatggcttg ttggtgccgt
     3241 cggcgaccag gtaaacgccg aactcgccct tgggcgcttc caccgccgca tacacctctc
     3301 ccgccgggac gtggaaccct tccgtataaa gcttgaagtg atggatcagc gcttccatcg
     3361 aagtcttcat ctcggcgcgg cgcggcggca cgatcttatt gtcggtcgaa accgacggcc
     3421 cggacggcat cttctcgatg cactgtttca tgattttcgt ggactggcgc atctcttcca
     3481 tgcgcatgac gtagcggtcg tagcaatcgc cgttcttgcc gaccggaatc tggaaatcga
     3541 ggtcgctgta gcattcgtag ggctgggcgc ggcgcaaatc ccaggccacg ccggtggagc
     3601 gcagcatcac gccggaaaaa ccgcgcgctt cggcttcttc gcggttgaag acggcgatat
     3661 ccacgttgcg ctgcttgaag atgcggttgt cggtgagcag ggtttcgatg tcgtcgagca
     3721 ccttggggaa ggtgccgcag aaggtgtaaa tatcttcgag cagttccggc ggcaggtcct
     3781 gatgcacgcc gccggggcgg aaataggccg cgtgcaggcg cgagccgctg gcgcgctcgt
     3841 agaacaccat cagcttctcg cgttcctcaa agccccacag gatcggcgtc agcgcgccga
     3901 catcgtttgc ctgggtggtg acgttgagca gatgcgacag cagccggccg atctcggcgt
     3961 acaacacgcg gatatattgg ccgcgcctgg gcacttccag gccgagcagt ttctcgatgg
     4021 cgaggcagaa ggcatgctcc tggttcatcg gcgcgacgta atcgagccgg tcgaaataag
     4081 gtatcgcctg caaataggtt ttgtgctcga tcagcttttc ggtgccgcga tgcagaagcc
     4141 cgatatgcgg gtcgatgcgg ctgacgatct cgccgtccaa ctccatcacc aggcgcagca
     4201 cgccgtgcgc ggcgggatgc tgcgggccga agttgatggt gattttgcca tccacgtcag
     4261 acatcacgaa cgcctttcgt ggtcagcgca gggcgaatag cgaatagaac cctcccctcc
     4321 ccgtgaggga gggtcgaaaa atccatagcg gagcgacgga tttttcgggg aggggtcgtg
     4381 cccataccct tgcgccaccc acccctcccc gaaaacatct tcgtgctgac gcgcgaatcc
     4441 gttttcgacc ctccctcaag gggagggtgg aagcggagac gatcgttcgc ataccttatg
     4501 agcattcgct attcgcctac tttcccgcct tctcgtcgcc cggaagaact tgcggcatcc
     4561 cttcccacgg actcatgaaa tcgaaatccc gatactcttg aaccaactgc accggctggt
     4621 agaccacgcg cttctggtcg tcgtcgtagc gcagttccac gaagccggtc agcggaaaat
     4681 ccttgcgcag cggatggccc tcgaaatcgt agtccgtgat gatgcgcctg aggtccgggt
     4741 gaccctcgaa gacgacgccg tagagatcga acgtctcgcg ctcgtaccag tccgccgccg
     4801 gaaaaacggg aatggcgctc ggcaccggcg ccgcctcgcc ggtctcgacc ttgacccgga
     4861 tgcgccggtt ctcggtcagc gaaagcagat gataaaccac gtcgaagcgc ttttcgcgcg
     4921 ccggccaatc cgcgccgcag atgtcgatca gcaccttgaa ctcgcactgg tcgcgcagga
     4981 acgtcagcac acgaacgatc tcgcccgctt cggcgtcgag cgtcaattcg ccgaacgcca
     5041 gcttgcttgc cttgatcgcg ccaggcagcg cgtccgcgat cttcgtggcg aggtcggtca
     5101 gcacagtcaa tgccgtcacc gctcgatcgt gccggtgcgg cggattttcc gctgcagcag
     5161 catgatgccg tacaccagcg cctcggcgct aggcggacag ccgggcacgt agatgtccac
     5221 cggcacgatg cggtcgcagc cgcgcaccac cgaataggaa tagtggtaat agccgccgcc
     5281 gttggcgcac gaacccatcg agatcacgta gcgcggctcc ggcatctggt cgtaaacctt
     5341 gcgcagcgcg ggcgccatct tgttgcacag cgtgccggcg acgatcatca cgtcggactg
     5401 gcgcggactg gcgcgcggcg cgaagccgaa ccgctccagg tcgtagcgcg gcatcgaagc
     5461 ctgcatcatc tcgatggcgc agcaggccag cccgaaggtc atccacatca gcgaaccggt
     5521 gcgcgcccag ttgatcagcg catcggcacc ggcgaccagg aaacccttgt ccgccagttc
     5581 gtcctgcatc gcctgaaaat agggatcgtt gtcggtgcgc gaaggcgccg ggatcactcc
     5641 cattagagcg ctcccttctt ccattcgtag atgaagccga cggtcagaac ccccaggaac
     5701 gccatcatcg accagaaggc gaaaacgccg gtgtcgccca gcgtgatcgc ccagggaaac
     5761 aggaaagcga cttcgagatc gaagatgatg aacaggatcg aaaccaggta gaaccgcacg
     5821 tcgaatttca tccgcgcgtc gccgaaggcc tcgaagccgc attcgtaggc ggagagcttt
     5881 tcggggtccg gcttggcggg cgcgatcacc aagggcacca cggcaaggcc gagcgcgatg
     5941 acccccgcga tcccgatgaa gatcagtatc ggcagatatt ccaggagcag ccgttccatt
     6001 cgttctttcc aactcccgcc gggggccgca cactggccca acccgtgccg gcaaaccagt
     6061 cgggctgcag catctcgcag tcgcactata gacggggatc gatccggcac aacgttgatt
     6121 tagaacgggt ttagagcagg aatcgccggg cgcaaacaag aatcttgggg agaattgctg
     6181 ccggcgcata gggatgaggc ggagtatgcg gcgaaggctg gcctgccggc cgtagccgcg
     6241 atgccaagct gtccgcctac gccaaggctt cggcggatgc gccttcaccg ctacgcggcg
     6301 aaggcaggcc tgccacccga agctgcgcga accaggacaa agccctccta cactctgaca
     6361 agctatggag ggcaccctcc gctgctgcgc agcgtagggt ggcgggagcg acggggctcg
     6421 aacctgaatt tcccgtattg cggaggaatc agcaggaggt aagtatttga aatagttgaa
     6481 tcgggttgtt gtgtacatct gccgattgct gcgaattggc cacgcgcggg cccccgaaaa
     6541 cattgcccta gaaaatgaac tcccccacgc ccgcagccgt accggaccgc ccgtgcaatt
     6601 cgcgaacgga tcaaacaggg gcacggccgc ctttggcaca tcgaagactt ccgcgacctg
     6661 ccagccgcag tcgcgagcag gcgccctccc gcctcgccaa ggctggtgtc atccgctgcc
     6721 tcagccacgg aacctattgt ctcgcgcggc agaccgcgct tgacgcgttc aggcccaatc
     6781 ccagggctgc tcgcgacctc gcctctaagt acaagtcggt gttgaaccaa ggtgatgaac
     6841 cgcgatccct tcgatctcga ccgctctttg cggaagtagc cgggaggctg gttctgccaa
     6901 gcccggaata gcatcaaagc cggggccgcg ttcagagcgg cttgaaatgc cgtcttgacg
     6961 cccatcttcc aacaacagca gcaaaaaaca gacggaagtt atagtccgcc cggaagcttg
     7021 gagcgcatca tctttttccg gtcgatcctg cgcccatccg tgacgaatgt gccgtcacca
     7081 acggcatgga tcgttctttt ttctttgcgg gagccgtcaa agtaggcggc aacgccttcg
     7141 agcacgatga tgctgtatcg atcgacgaca tcgacaaccc ggcattcgat attggcgaga
     7201 cactccttga tgagcggcgc ccgaacatgc ttggccttca cacgggtgag gccgaacttt
     7261 tcgaacttgt ccgtatcttt cccggagcat gttcccacgc cgacgaccgt atccatcagg
     7321 tccaccgtcg gaagcgaaat cacacacgcc ttcgatttcc gaagcgcggc ataggaatag
     7381 ttccaaggcc cggtcgtaat ggcaaaggac gccgaaaagt ccagcaccat cgtccaggag
     7441 atggtcatca cattatcttt tcgaccgtcg tttgtggtga ccaggatgac cggcccgggc
     7501 tccatcagcg tgaaggc
//
LOCUS       DASWPU010000450         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_9435, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000450 DASWPU010000000
VERSION     DASWPU010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9435"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VGC27_09635"
     CDS             complement(<1..417)
                     /locus_tag="VGC27_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY0282871.1"
                     /translation="MSARTVKHYLDIADLDAATLHELIAEARRRKNARAGLPHGAADA
                     DRPLEGRVLAMIFDKQSTRTRISFDVGMRQLGGTTIMLTGSEMQLAREETIADTARVI
                     SRYVDAVMIRLLDHEMVLDLAENAAIPVINGLTKLSH"
     gene            complement(414..1610)
                     /locus_tag="VGC27_09640"
     CDS             complement(414..1610)
                     /locus_tag="VGC27_09640"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0282872.1"
                     /translation="MFPSLLPTYNRADVAFVRGEGPYLFAEDGQRYLDFGAGIAVNAF
                     GHAHPKLVAALTEQAGKLWHTSNLYRVPGQESLSKRLVENTFADTVFFTNSGVEACEL
                     AFKMARRYQYVSGHPGRFRIITFEGAFHGRTLAGIAAGGQAKYLEGFGPKVEGFDSIP
                     VGDLKAVKAAIGPATAAILVEPVQGEGGVRVLPAEFLRGLRALCDEHGLLLIFDEIQT
                     GVGRTGKLFAYEWLGITPDIMPIAKGIGGGYPLGACLATAEAAKGMTAGTHGSTYGGN
                     PLGMAVGNAALDLVLAPDFLPHVEAIGNYLRQQLAGLLAEYPAVFEEVRGQGLLLGLK
                     LRVPVPEFVAAARKQGLLLVGAGDNVVRLLPPLIIEEAQVREALDKLSAAAKGFGTSS
                     KKTVAA"
     gene            1909..2358
                     /locus_tag="VGC27_09645"
     CDS             1909..2358
                     /locus_tag="VGC27_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEY0282873.1"
                     /translation="MTQANWTDDRVEQLKNLWTEGLSASQIARVLGGVTRNAVIGKVH
                     RLGLAGRASPSRVERPRLPSTPRVPIRSHIPPAPVVEEDPLPLDDGNFATVLTINDRM
                     CRWPIGDPSENEFHFCGRKPKSGSPYCEAHARKAYQPQQQRRDKMAG"
     gene            2402..2569
                     /locus_tag="VGC27_09650"
     CDS             2402..2569
                     /locus_tag="VGC27_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282874.1"
                     /translation="MRKHVEKPKARRAPVKEKAQDARMAGRDRIKTAESPVLQLEKLL
                     HDTYARRACHL"
     gene            complement(2585..>3200)
                     /gene="phoB"
                     /locus_tag="VGC27_09655"
     CDS             complement(2585..>3200)
                     /gene="phoB"
                     /locus_tag="VGC27_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY0282875.1"
                     /translation="VLEAQDGEEALLVVAEEKPDLVLLDWMLPQLSGIEVCRRLRGRQ
                     ETRNVPIIMLTARGEETDRIRGLDTGADDYLTKPFSMIELLARLRAVLRRIRPSLAED
                     VVRAGDIEMDRAAHRVRRSGKEVHLGPTEYKLLEHLIQHPGRVFSREQLLDAVWGSDV
                     YVEARTVDVHVGRLRKALNLEGVRDPVRTVRSAGYALEDGAVTA"
BASE COUNT          579 a         1054 c         1018 g          549 t
ORIGIN      
        1 atgcgaaagc ttggtcagtc cgttgatgac gggaatggcc gcgttctcgg ccagatccag
       61 caccatttcg tgatcgagca ggcggatcat cactgcgtcc acgtagcgcg aaatcacccg
      121 cgcggtatcg gcgatggttt cctcgcgggc gagctgcatc tcgctgcccg tcagcatgat
      181 cgtggtgccg cccaactggc gcatcccgac atcgaaggaa atgcgcgtgc gggtggactg
      241 cttgtcgaag atcatcgcca gcacgcgccc ttcgagcggg cggtcggcgt cggcggctcc
      301 gtgcggcagg ccggcccgcg cattcttgcg acggcgcgcc tcggcgatca gctcatgcag
      361 cgtagccgca tcgagatcgg cgatgtcgag atagtgcttg accgtccggg cgctcatgcc
      421 gccaccgttt tcttgctgga ggttccaaag cccttggcgg cggcggacag cttgtccagc
      481 gcctcgcgca cctgggcttc ctcgataatc agcggcggca gcagccgcac gacattgtcg
      541 cccgctccaa ccaggagcaa gccctgcttg cgcgccgccg cgacgaattc gggaaccgga
      601 acgcgcagct tgaggccgag caacagcccc tgtccgcgca cttcctcgaa taccgcggga
      661 tattcggcga gcagcccggc aagctgctgg cgcagataat tgccgatggc ttccacgtgc
      721 ggcaggaaat ccggcgccag caccagatcg agcgcggcgt tgcccaccgc catgccgagc
      781 ggattgccgc cataggtcga gccgtgcgtg cccgcggtca tgcctttggc cgcttcggcg
      841 gtggcaaggc aggcgcccag cggatagccg ccgccgatgc ccttggcgat cggcatgatg
      901 tcgggcgtga tgcccagcca ttcgtaggcg aagagcttgc cggtgcggcc gacgccggtc
      961 tgaatctcgt cgaagatcag cagcaatccg tgctcgtcgc acagcgcgcg caagccgcgc
     1021 aggaattccg ccggcagaac ccgcacgccc ccttcgccct gcaccggctc gaccaggatc
     1081 gcggcggtcg cgggcccgat ggcggctttc accgctttca gatcgcccac cggaatggaa
     1141 tcgaagcctt ccaccttggg gccgaaacct tccagatatt tcgcctgccc gcccgcggcg
     1201 atgcccgcca gggtgcggcc gtgaaaggcg ccttcgaagg tgatgatgcg gaaccgcccg
     1261 ggatggccgc tgacatactg gtagcgccgc gccatcttga atgcgagttc gcaggcttcc
     1321 actcccgaat tggtgaagaa cacggtgtcg gcaaaggtgt tttcgaccag ccgcttggac
     1381 agcgattcct gccccggcac gcgatagagg ttcgaggtgt gccagagctt gcccgcttgt
     1441 tcggtgagcg ccgccaccag cttggggtgc gcatgaccga aggcgttgac cgcgatgccg
     1501 gctccgaaat cgagatatcg ctgcccatcc tcggcgaaca gatacggtcc ttcgccccgg
     1561 acgaatgcga catccgcccg attgtaggtt ggcagcaatg acggaaacac ctgatcctcc
     1621 tctcggttct gcgctcttgt ggataaccca ttcgcgcggg cgggctttat ccgctttctg
     1681 cctttgccgc acaagcgagg cgctttggcg ggccaccatg cacattccac agccttggaa
     1741 tcacgcatga tttttgatgt ggaacgaatc tgccgaagaa aattggtact gagtcggttt
     1801 ctgtggacga agcggtggta ggcgttccac acgacttgtg cgtccctttg cggcggctac
     1861 gatatcttgt ggccggccaa aagccggcaa gcccgaaagg aagacgccat gactcaggcg
     1921 aattggaccg acgaccgcgt tgaacagctc aagaatctct ggaccgaggg actctcggca
     1981 agccagatcg cccgcgtact cgggggtgtg acccgcaacg ccgtcatcgg caaagtacac
     2041 cgtcttggcc ttgccggccg cgccagccct tcgcgcgtcg aacgcccgcg cctgccgagc
     2101 acgccgcgcg tcccgattcg ctcgcatatc ccccccgcgc cggtggtcga ggaagacccc
     2161 ctccccctgg atgacggcaa tttcgccacc gttctgacga tcaacgaccg catgtgccgc
     2221 tggcccatcg gcgatccgtc cgagaacgag ttccatttct gcggccgcaa gcccaagtcg
     2281 gggtcgccct attgcgaagc gcatgcgcgc aaggcctacc agcctcagca acagcgtcgc
     2341 gacaagatgg cgggatgagg cggaaggaga cgacgcccca gcttcgtgct aggatttccg
     2401 catgaggaaa catgtggaaa aaccgaaggc aagacgcgct ccggttaaag aaaaagcgca
     2461 agacgcgcgc atggcaggtc gggatcggat caagaccgcg gagagcccgg tattgcagct
     2521 tgaaaagctg ctgcacgaca cctacgcgcg cagggcttgc cacctgtaac cggcgagcgg
     2581 cccgtcaggc ggtaacggcg ccgtcttcaa gcgcgtagcc ggccgaacgc acggtgcgga
     2641 cggggtcgcg cacgccttcg aggttgagcg ccttgcgcag gcgcccgaca tgcacgtcca
     2701 cggtgcgggc ttcgacataa acgtccgatc cccacaccgc gtcgagcagt tgctcgcgcg
     2761 aaaacacccg gccgggatgc tggatcaggt gttcgagaag cttgtactcg gtcgggccca
     2821 gatggacttc cttgccgctg cggcgcacgc ggtgggcggc gcggtccatt tcgatgtcgc
     2881 cggcgcgaac cacatcctcg gccaggctcg gacggatgcg ccgcagcacc gcgcgcaggc
     2941 gggcgagcaa ttcgatcatc gagaagggct tggtgagata atcgtcggcg ccggtatcga
     3001 gcccgcgaat gcggtcggtt tcctcgccgc gcgccgtcag catgatgatc ggcacattgc
     3061 gggtttcctg ccgtccgcgc aggcgccggc agacctcgat gcccgaaagc tgcggcagca
     3121 tccagtcgag cagaaccaga tcgggcttct cctcggcgac gaccagcaac gcctcttcgc
     3181 cgtcctgtgc ttccagaacg
//
LOCUS       DASWPU010000451         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_46905,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000451 DASWPU010000000
VERSION     DASWPU010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46905"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VGC27_09660"
     CDS             complement(<1..431)
                     /locus_tag="VGC27_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0282876.1"
                     /translation="MTTPEEKTLLIVEDDRPLRERLARAMESRGFVVTIAEGVAEGKA
                     RVKEAPPAYAVVDLKLEDGNGLDVLETLHTVRPDSRVVVLTGFGNIATAVAAVKYGAI
                     DYLPKPADADDIMAALLAPPGSKPKPPENPMSADRVRWEHI"
     gene            complement(480..1880)
                     /locus_tag="VGC27_09665"
     CDS             complement(480..1880)
                     /locus_tag="VGC27_09665"
                     /inference="COORDINATES: protein motif:HMM:NF033792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HEY0282877.1"
                     /translation="MTSTDTETRSQRARMMPVARASAVRGRVRLRTLSNLRWMATAGQ
                     TTALFVVYFGFGYSLPLVQCLTCVAFSAGLNIVLALRYPPSHRLTNREATVYLAYDVL
                     QLAALLYLTGGIANPFALMFVAPVVIAASTLNLGNTLILGCIALASASLIAVAHEPLP
                     WSQDPQLILPPLYQAGIWASLVLGIGFTSIYAWRIASEGARMSAGLAATQLALAREQR
                     LSSLGALATAAAHELGSPLGTIAVVAHELERVLPVGSQEAEDVRLLRSQAERCRMILM
                     RLARPEEAMLGATARLPLCALLDDIADTYRGEDLDIVIAPAPVQRDEPQPQVWRAPEL
                     LHGLGNIIENAADFANSRVRIEASWGPATLRVTVEDDGPGFSSDIIERIGEPYITSRP
                     GHDALGATEMGPPTAPLGKREGMGLGFFIAKTLLEQTGGTVKAVNLTEGGARVSVSWP
                     RGQIDGETPLTRAQED"
BASE COUNT          348 a          653 c          661 g          346 t
ORIGIN      
        1 tggatatgct cccagcgcac gcggtcggcc gacatcggat tttccggcgg cttgggcttg
       61 gagccgggag gcgccagcag cgccgccatg atgtcgtcgg cgtcggcggg cttgggcaga
      121 taatcgatgg cgccgtattt cacggcggcg accgcggtcg cgatattgcc gaagccggtc
      181 agcaccacca cgcggctgtc gggacgcacc gtgtgcaaag tctccagcac gtcgagtccg
      241 ttgccgtctt ccagcttgag atcgaccacc gcatacgccg gaggggcttc cttgacgcgg
      301 gctttgcctt cggcgacgcc ttcggcgatc gtcaccacga agccgcgcga ttccatggcg
      361 cgggccaaac gctcgcgcaa cgggcggtcg tcttccacaa tgagcaatgt cttttcttca
      421 ggcgtggtca tggtccctgt ccttggtcgt tgcgcaaata ggcccggcgg cagaaggcgt
      481 caatcctcct gtgccctcgt caatggtgtc tctccgtcaa tttggccgcg cggccacgat
      541 acgctgacgc gcgcgccacc ttcggtaagg tttaccgcct taaccgtacc gcccgtctgc
      601 tccagcaggg ttttggcgat gaagaagccc agccccatgc cttcgcgctt gcccagcggt
      661 gcggtgggcg gccccatttc ggtggcgccc aaggcatcgt ggccgggccg cgaagtaatg
      721 taaggctctc cgattcgttc gatgatatcg gacgagaatc ccggcccgtc gtcctcaacc
      781 gtgacgcgca gcgtggcggg gccccagctg gcctcgatgc gcacgcggga gttggcgaag
      841 tcggcggcgt tctcgataat gttgccgagc ccgtgcagca gttcgggggc gcgccagact
      901 tgcggctgcg gctcgtcgcg ctggaccggc gcgggggcga tgacgatgtc cagatcctcg
      961 ccgcgatagg tgtcggcgat gtcgtcgagg agggcgcaca gcggcagacg cgcggttgcg
     1021 cccagcatcg cttcctcggg acgggccagg cgcatcagaa tcatgcggca gcgttcggcc
     1081 tggctgcgca gcaggcggac atcctcggcc tcctgcgagc ccacgggcag aacgcgctcc
     1141 agttcgtgcg ccaccaccgc gatggtcccc agcggcgagc ccagttcatg ggccgcggcc
     1201 gtagccagcg cgcccaaaga cgacaggcgc tgttcgcgcg ccagcgccag ttgcgtagcg
     1261 gcaagaccgg ccgacatccg cgcgccttcg ctggcgatgc gccaggcata gatcgaggtg
     1321 aagccgatgc cgagcaccag cgaggcccat atgcccgcct gataaagcgg cggcagaata
     1381 agttgcgggt cctgcgacca gggcagcggc tcgtgcgcca ccgcgatcaa cgatgccgat
     1441 gccagtgcga tgcaacccag gatcagcgtg ttgccgagat tgagtgttga cgccgcaatc
     1501 accaccggcg cgacgaacat caaggcgaaa ggattggcga tgccgccggt gagataaagc
     1561 agcgccgcca gttgcaacac gtcgtaggcg agatagaccg tggcttcgcg gttggtgagc
     1621 cggtggctcg gcggatagcg caaggccagc acgatgttga ggccggcgct gaaggccacg
     1681 caggtcaggc attgaacgag cggcaggcta taaccgaaac cgaaatagac cacgaacaag
     1741 gccgtggtct gcccggccgt cgccatccag cgcagattcg acaaggtgcg caggcgcacg
     1801 cgccctctga ccgccgaagc ccgcgcgacg ggcatcatcc gcgcccgctg ggaccgtgtt
     1861 tccgtatccg tcgacgtcat ggcctagccg gttgcctttt ccgcgcgcaa tactatcaat
     1921 caattctatc ggaccgagtc tcacgcttgg aacgcaatac gtaatgatcc gtacgccgct
     1981 tgccctgatt ccctatctta tcgcggtg
//
LOCUS       DASWPU010000452         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_58880,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000452 DASWPU010000000
VERSION     DASWPU010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58880"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>1550
                     /locus_tag="VGC27_09670"
     CDS             18..>1550
                     /locus_tag="VGC27_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282878.1"
                     /translation="MSTAPGQEAQDAKILGLTNFIDLSNVPKYSGGSADARTEQDNQK
                     LFALYTAISNLSYLATMSQREGMTEGQLAGFDQRFQMGLQQIESFIGKATFNNFTLQA
                     ASSSASITSTIKVPSATFGYTGGTIVKSENIAAPVAGVGADDSFTISIAKNGTTTEVL
                     IDLSQVQGPLTIDNIVEYVNGQLAAGGFSTRFKRTMTEGTIVDPSKAAYGIEISTSST
                     ESVTLSSVAAVPALYVAGVSGNTVSTAAAAADNQGRLVKLTDLNDPQGVFNATVKPTT
                     GTTTAQATAVDANGDVYVVGNATGDFGNQLNQGSQDVYLTKYDSAGNLQWVSLLGSAG
                     TASAYSLSIDPTGGVVVAGSTNAHLTPTSISNGNTGSFVVKYDANGHQSWTTQIQTLA
                     NNRAATVSVDASGNVFVGGQVTGMIGAGQTNSGGSDAYLAKFDSKGKLVYENQFGTAE
                     NDAVAATAVTASGDLVVATVQNGHAILAKYAGGDATAAPVWQTDLGDLQGSGAITGLA
                     VSG"
BASE COUNT          328 a          497 c          470 g          255 t
ORIGIN      
        1 acgacagggt gccttggatg tctacggcgc cgggccaaga ggcgcaggac gccaagatcc
       61 tcgggctgac gaacttcatc gacctttcca acgtaccgaa atattccggc ggctcggccg
      121 acgccaggac cgagcaggac aatcagaagc tcttcgccct ctatacggcg atcagcaatc
      181 tgtcctatct cgccacgatg tcccagcgcg aaggaatgac ggagggccaa ctggccggct
      241 tcgaccagcg cttccagatg ggattgcagc agatcgagtc gttcatcggc aaggcgactt
      301 tcaacaattt cacgcttcag gcggcgtcgt ccagtgcatc gatcaccagc acgataaagg
      361 tgccgtccgc gacattcggt tataccggcg gcacgatcgt caagagcgaa aacatcgccg
      421 ctccggtcgc aggtgtcggt gccgacgaca gtttcacaat ctccatcgcc aagaacggga
      481 cgacgacaga ggttctgatc gacctttcgc aggtgcaggg acctctcacg atcgacaaca
      541 tcgtcgagta cgtcaacggc caattggccg ccggcgggtt ctccacgcgg ttcaagcgga
      601 cgatgaccga aggcaccatc gtcgatcctt caaaggccgc ttatgggatc gaaatcagca
      661 cctcttcaac cgaaagcgtc acgctgtcct cggttgcggc cgtgccggcg ctctatgtgg
      721 ccggggtttc gggaaacacg gtgtcgacgg ctgcggctgc ggcggacaat cagggcaggc
      781 tcgtcaagct caccgatctg aacgatccgc aaggcgtgtt caacgccacc gtcaagccga
      841 cgacgggaac cacgacggcg caagcgacgg cggttgacgc caacggcgat gtctatgtcg
      901 tcggaaacgc caccggcgac ttcggcaatc agctcaatca gggttcgcaa gacgtctatc
      961 tgacgaagta cgattccgcc ggcaacctgc aatgggtgag ccttctgggc agcgcgggca
     1021 cggcttcggc ctattccctc agcatcgatc ccaccggggg cgttgtcgtc gcaggttcga
     1081 ccaacgccca tctcacgccc acctcgatca gcaacggcaa caccggcagc ttcgtcgtca
     1141 aatacgacgc caacggccac cagagctgga ccacgcaaat ccagaccttg gcgaacaacc
     1201 gcgccgccac ggtgagcgtg gatgcatccg gcaatgtctt tgtcggcggg caagtcaccg
     1261 gcatgatcgg cgccgggcag accaattcgg gcggcagcga cgcgtacctg gccaagttcg
     1321 attccaaagg caagctcgtt tacgaaaacc agttcggcac cgcggaaaac gacgcggtcg
     1381 cggcgaccgc cgtcacggcg tccggcgatc tggtggtggc gaccgtacag aacggccacg
     1441 ccatcctcgc caaatacgcg ggcggcgacg cgacggcggc gccggtttgg cagaccgatc
     1501 tgggcgatct gcaaggcagc ggcgcgatca ccggcttggc cgtgtcgggc
//
LOCUS       DASWPU010000453         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_9464, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000453 DASWPU010000000
VERSION     DASWPU010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9464"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VGC27_09675"
     CDS             complement(<1..1014)
                     /locus_tag="VGC27_09675"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0282879.1"
                     /translation="MSGRAISARFAGRAAEIGEAAWNACANPGGGTDPHPFTRHEFFV
                     ALEESGSADVKTGWRPLHLVIERDGRAEGILPLYLKGHSLGEYVFDHAWADALERVGG
                     VYYPKLQSCVPFTPVTGRRLLVAAGADEATTRTALLAAGAAAVEQLSVSSLHITFPTE
                     EEWQAAGEFGYLQRADRQFHWENRGYASFDEFLGDLSSSRRKNLRKERASIREAGIEF
                     DWLTGRDITEAHWDAFFEFYIDTGGRKWGDPYLTREFFSRIGSMSEQILLVMARRGGR
                     TIAGALNLFGEGVLYGRNWGCIEYVPFLHFETCYYQAIDFAIAHGLKKVEAGAQGEHK
                     LLRG"
     gene            complement(1011..1478)
                     /locus_tag="VGC27_09680"
     CDS             complement(1011..1478)
                     /locus_tag="VGC27_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0282880.1"
                     /translation="MTGRIESRLKELGIALPVPPAPVASYVPTVASGNLVFVSGQITS
                     TPDGLKYVGVVGKDLTVEDGKAAARLCAINAVAQAKAACGGDLDRVQRCVKLSVFVNA
                     APDFTQHPEVANGASDLLVEIFGEAGKHARAALGAGSLPRGVACEVEALFEIV"
BASE COUNT          311 a          549 c          495 g          287 t
ORIGIN      
        1 gccgcgcaac agcttgtgct cgccttgagc gccggcttcc accttcttca ggccgtgcgc
       61 gatggcgaag tcgatggctt gataatagca ggtctcgaag tgcaggaacg gcacatattc
      121 gatgcagccc cagttgcggc catagagcac gccttcgccg aacaggttga gcgcgcccgc
      181 gatagtgcgc ccgccgcgcc gcgccatcac cagcaaaatc tgctccgaca tgctaccgat
      241 gcggctgaag aattcgcgcg tgaggtaggg atcgccccat ttgcgcccgc cggtgtcgat
      301 gtagaactcg aagaaggcgt cccaatgggc ctcggtgatg tcgcggccgg tcagccagtc
      361 gaattcgatg cccgcttcgc ggatgctggc gcgctccttg cgcagatttt tgcgcctgga
      421 cgaagacagg tctcccagaa attcgtcgaa gctggcgtag ccgcgattct cccaatgaaa
      481 ttgacggtcg gcgcgctgca gatagccgaa ctcgccggcc gcctgccatt cttcttcggt
      541 ggggaaagtg atgtgcagcg acgagaccga gagttgctcc accgccgccg cgcccgcggc
      601 cagcagcgcc gtgcgcgttg tcgcctcgtc tgcccccgcc gcgaccagca gccgccgtcc
      661 cgtcaccggc gtgaacggaa cacaggactg gagtttggga taataaaccc cgcccacgcg
      721 ctccagcgcg tccgcccagg catgatcgaa gacatattcg cccagcgaat ggcctttcag
      781 atagagcggc aggatgcctt ccgcgcgccc gtctcgttcg atcaccaggt gcagcggacg
      841 ccagcccgtc ttcacgtcag ccgatccgga ttcctccagc gcgacgaaga actcatggcg
      901 cgtgaacgga tgcgggtcgg taccgccgcc aggattggcg caagcattcc atgccgcttc
      961 cccgatctcg gcggcgcggc cggcaaagcg cgcggaaata gctctcccgc tcacacgatc
     1021 tcgaacaagg cttccacctc gcacgccacg ccgcgcggca gcgatccggc gcccagcgct
     1081 gcgcgcgcat gttttccagc ctcgccgaat atctccacca gcagatcgga ggcgccgttg
     1141 gcgacttccg gatgctgggt gaaatcaggc gccgcgttca cgaatacgct gagcttgacg
     1201 cagcgctgca cgcgatcgag atcgccgcca cacgcggcct tggcctgggc cacggcattg
     1261 atggcgcaca gccgcgccgc cgccttgccg tcttccaccg tcaaatcctt gcccacgacg
     1321 ccgacatatt tcagaccgtc cggcgtggag gtgatctgtc cggagacgaa caccagattg
     1381 ccgctggcga ccgtcggcac atagctcgcc accggagcgg gcgggaccgg cagagcgatc
     1441 cccaattcct tcaagcgcga ttcgatgcga ccagtcattt gcgatcccct tctgtcgtaa
     1501 aactatagcg aaaagaacag atatgcgaag cccgccttcg aaagaactac ggcgggtccg
     1561 tggatcatgt aggtccgccg aagctttttg cgtaggcgga actttccgtg agtggaggca
     1621 cacgctatag aggacgcatg gt
//
LOCUS       DASWPU010000454         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_52692,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000454 DASWPU010000000
VERSION     DASWPU010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52692"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /gene="ettA"
                     /locus_tag="VGC27_09685"
     CDS             complement(<1..1472)
                     /gene="ettA"
                     /locus_tag="VGC27_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY0282881.1"
                     /translation="MAGPQYVFVMKGLSKTYPGGRQVLKDIWLSFYPGAKIGIVGVNG
                     AGKSTLMRIMAGLDKEFTGEAWAADGIRMGYLPQEPQLDPAKDVRGNVMEGVAAKQAI
                     IDRYNEIAANYSDETADEMARLQDEIEAKGLWDLDSQIDQAMDALRCPDGDADVTALS
                     GGEKRRVALCKLLLDAPDILLLDEPTNHLDAESVGWLEHTLCEYKGCVILVTHDRYFL
                     DNVTGWILELDRGKGIPHEGNYSSFLVVQEKRLYQEGQEGAARERTIAREREWIQQSP
                     RARQAKSKARINAYEELLAKSQEQRGGPAQIVIPVAERLGSLVVETEGLMKGFGDREL
                     FEDVNFNLPPGGIVGVIGPNGAGKTTLFKLITGQEKPDAGSLRIGDTVRIGYVDQSRD
                     SLDPKKTVWEEISGGLDILELGKRSVQSRAYVGAFNFKGGDQQKKVGQLSGGERNRVH
                     LAKMLKSGANLLLLDEPTNDLDVETLRALEMALEEFAGCA"
     gene            complement(1577..>2317)
                     /locus_tag="VGC27_09690"
     CDS             complement(1577..>2317)
                     /locus_tag="VGC27_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282882.1"
                     /translation="GGAYVGMDMALARGLRVSFGQASSAPGYNPYLLSPRLALAGIGG
                     SGLPYDGRSTNSLLAGVSWNFARWGGLNFTASQTTERGGVLGENNAAVDLARTSALGL
                     SAHVGFGGGWVTTASYSEGTTQLGLRPSAFSAGVESELRVHSYGIAVAKHGLFGNDAL
                     GVAFSRPAPNYGGGTFSGANSNDMQFFNRDKLFAGTTPETDIELGYVTNFFGNSVALQ
                     ANASYQTNLGGQNGVNSMSLLSRAKIKF"
BASE COUNT          384 a          763 c          735 g          435 t
ORIGIN      
        1 atggcgcagc cggcgaattc ctccagcgcc atttccagcg cccgcaaggt ctcgacgtcc
       61 agatcgttgg tcggctcgtc gagcaggagc agattggcgc ccgacttcag catcttggcg
      121 agatggacgc ggttgcgttc gccgcccgaa agctggccga ccttcttctg ctggtcgccg
      181 cccttgaagt tgaaagcccc gacataagcg cgcgactgca cgctgcgctt gcccagttcc
      241 agaatgtcga ggccgccgga aatctcctcc cacacggtct tctttgggtc gagtgaatcg
      301 cgcgactggt cgacatagcc gatccgcacc gtatcgccga tgcgcaagct gccggcgtcg
      361 ggcttttcct ggccggtgat cagcttgaac agcgtggtct tgcccgcgcc gttggggccg
      421 atgaccccga cgatgccgcc cggcggcagg ttgaaattca catcctcgaa cagttcgcgg
      481 tcgccgaagc ctttcatcag gccctcggtt tccaccacca atgagcccag acgttcggcc
      541 accggaatga cgatctgcgc cggcccgccg cgctgttcct gcgacttggc gagcagttcc
      601 tcataggcgt tgatgcgggc cttggacttc gcctggcggg cgcgcgggct ttgctgaatc
      661 cattcgcgtt cgcgcgcgat ggtgcgctcg cgggcggcgc cctcttggcc ttcctgatac
      721 agccgcttct cctgcaccac gaggaacgac gaataattgc cttcgtgcgg gatgcctttg
      781 ccgcggtcga gttccagaat ccagccggtg acgttgtcga ggaaatagcg gtcgtgggtg
      841 accaggatca cgcagccctt gtattcgcac agcgtgtgtt cgagccagcc cacggattcg
      901 gcgtccagat ggttggtcgg ttcgtcgagc agcaggatgt cgggggcgtc gagcagcagc
      961 ttgcacagcg ccacgcggcg cttctcgccg ccggacagcg cggtgacgtc ggcgtcgccg
     1021 tcggggcagc gcagcgcgtc catcgcctga tcgatctggc tgtcgaggtc ccacaagccc
     1081 ttggcttcga tttcgtcctg caagcgcgcc atttcgtcgg cggtctcgtc ggaatagttc
     1141 gcggcgatct cgttgtagcg gtcgatgatc gcctgcttgg ccgccacccc ttccatgacg
     1201 ttgccgcgca cgtccttggc cggatcgagc tgcggctcct ggggcagata gcccatgcgg
     1261 atgccgtcgg cggcccaggc ttcgccggtg aattccttgt cgaggcccgc catgatgcgc
     1321 atcagggtgg acttgcccgc gccgttgacg ccgacgatgc cgatcttggc gccggggtag
     1381 aacgacagcc agatatcctt cagcacctga cggccgccgg gataggtctt cgacaacccc
     1441 ttcatcacga acacgtattg cggcccggcc atcgtcggtt cctttgcggg agggatgtgg
     1501 ggttttagaa tgctggctgg cagggtcaag cccggctgcg ccccaggctt cgccgggcgg
     1561 gcccggccgg gaacggctag aacttgatct tcgccctcga cagcagcgac atcgaattga
     1621 cgccgttctg gccgcccaaa ttcgtctgat aggaggcgtt ggcctgaagg gcgaccgaat
     1681 tgccgaagaa gttggtgaca tagccgagtt cgatgtcggt ttccggcgtc gtgcccgcaa
     1741 acaacttgtc gcggttgaag aactgcatgt cgttcgaatt cgcgcccgag aacgtgccgc
     1801 cgccgtaatt cggcgccggg cgcgagaacg ctacgccgag cgcatcgttg ccgaacaggc
     1861 cgtgcttggc caccgcgatg ccgtaggaat gcacgcgcag ctcggactcc acgccggcgc
     1921 tgaaggcact gggcctcagg ccaagctgcg tggtgccttc gctgtacgac gccgtcgtca
     1981 cccaaccgcc gccgaagccc acatgcgccg acaaaccgag cgccgaggtt cgcgccagat
     2041 cgactgccgc attgttttcg cccaaaacac cgccacgttc ggtcgtctgc gacgccgtga
     2101 aattaagccc gccccatctc gcgaaattcc acgacacgcc ggcgagcaac gaattcgtgc
     2161 tgcgcccgtc atagggaagg ccggagccgc cgatcccggc cagcgccaga cgcggcgaga
     2221 gcagataagg attgtagccg ggcgcgctgg aagcttgacc gaaactgacg cgcaggccgc
     2281 gggccaacgc catgtccatg ccgacatacg ctccgcc
//
LOCUS       DASWPU010000455         8137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_108283,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000455 DASWPU010000000
VERSION     DASWPU010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108283"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1120
                     /gene="dusB"
                     /locus_tag="VGC27_09695"
     CDS             110..1120
                     /gene="dusB"
                     /locus_tag="VGC27_09695"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY0282883.1"
                     /translation="MDPAFRIGDVTIPGRVLTAPMSGVSDLPFRCAASKLGAPYVVTE
                     MVACETLASGRPDVVRRAAVGEGLPLMVVQIVGREACWIAQGAAMAEASGAQIIDLNM
                     GCPAREVTGGLCGSALMRDLDQAERLISAAVGATSRPVTLKMRLGWDDASRNAPQLAA
                     LAERCGVKAITVHGRTRQQFYKGPSDWRAVAEVKAATSLPVIVNGDVVDDVSAREALR
                     QSGADAVMIGRGACGRPWIAAAVERALRTGGAMIEPSLAQRLDIALDHFRDSLRFYGD
                     ALGLKIFRKHLGWYVEGADRPQGAQTRRAAKSRLCRLTQPGEVEAGLIALWGTAQRGT
                     LQ"
     gene            1117..1422
                     /locus_tag="VGC27_09700"
     CDS             1117..1422
                     /locus_tag="VGC27_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282884.1"
                     /translation="MTKDELMAWALANGWQMMGGYPSLPKPRQPDVAIVRLVLKATVA
                     SLEIRKPTGKWEKVAGESYSKIAADPDNGMPSGLGFATINGLSTLMRDNKDRLVFAK"
     gene            1522..1923
                     /locus_tag="VGC27_09705"
     CDS             1522..1923
                     /locus_tag="VGC27_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282885.1"
                     /translation="MTKSIRTTLLLGVLDWLIPFAISFAVFPLKALNYFLFESVMTIA
                     IVFAAILLGVIYFKDLKDTTANFVRAGLLLGLIWLAINLAADLLIFLPKSPMQIPLPL
                     YMSQIGVKYLSIPIITAGLGYLKKQAGQAAG"
     gene            complement(2297..3706)
                     /gene="uxaC"
                     /locus_tag="VGC27_09710"
     CDS             complement(2297..3706)
                     /gene="uxaC"
                     /locus_tag="VGC27_09710"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEY0282886.1"
                     /translation="MTSALRLHPDRFFPTDSGSATIARRLFAHVETLPIISPHGHTDP
                     SWFANDAPFEDATSLFLWPDHYVHRMLYSQGITLAQLGIAPAGGTAEKDRRASWRLLA
                     ENYWLFRGTPSRTWLDHAFATVFGIEVRLEAATADLYYDAITEALKKPEFRPRALFKR
                     FNIEALATTEGANDPLLHHTKLQSEWDGKVVTTFRPDGVVDPDARGFAANVAELGALT
                     KENTATWKGYLAALANRRAFFRTHGATATDHGHPTARTADLPANECQGLLDRALKGTL
                     APGEADLFRAQMLTEMALMSCEDGMTMQIHPGAFRNHNKTLFAGYGPDRGADIPMRTS
                     YVDALKPLLDRVGNRADLTVILFTLDESNYARELAPLAGHYPCLRLGPAWWFHDSPEG
                     MMRYFRQTTETAGFYNTAGFNDDTRAFFSIPARHNLYRRVMSAYLAELVATHRLDETE
                     AAETAVDLAYKLPKKAYKL"
     gene            4118..4432
                     /locus_tag="VGC27_09715"
     CDS             4118..4432
                     /locus_tag="VGC27_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282887.1"
                     /translation="MRSRKSVIFVPGPLVVLTGLTMLRAVPSPTQITRHDCVEGWSCI
                     GEWTGTPLSLILAAAKPKTNARLSCVPLRRRHGSGREAAQYYYESLDLLEAIYPPDNS
                     GL"
     gene            4743..5477
                     /locus_tag="VGC27_09720"
     CDS             4743..5477
                     /locus_tag="VGC27_09720"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY0282888.1"
                     /translation="MAYRSWFLAVALLSVLTAGCATSNPESLAQNDPYEPTNRAVFKV
                     NMAIDNAVAKPVAKFYNRALPVPVRNGIHNFLTNLNKPVTFGNDVLQGEPVRASETLV
                     RFTLNSTLGIAGLFDVASTLGVPDHSEDFGQTLGVWGAGEGPYLVLPFIGPDPPRDLT
                     GNVADIFMDPFTYINFHGSDTWYAVRTGVAVLDLRARNVDTLEQIERTSIDLYATTRS
                     LYRQYRNAEIRNGAPEPQDTSDTPEF"
     gene            complement(5474..>8137)
                     /locus_tag="VGC27_09725"
     CDS             complement(5474..>8137)
                     /locus_tag="VGC27_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0282889.1"
                     /translation="GPLRYQIYVNDQAREASDFRSLVLAYREGAAVRLSDVGKVYEGV
                     EDVRNLGMANGKPAILVILFRQPGANIIETVDNVKAELPRLQKALPSSIRLIIANDQT
                     TSIRASLHDMEITMVIAILLVMFVVYLFLRNGRAALIPCVAVPLSLIGTFTVMYLLGY
                     SLDNLSLMALTVATGFVVDDAIVVLENVTRYIEQGMSRFEATLKGAREVGFTVLAMSL
                     SLIAVFLPILLMGGIIGKFFQEFSVTLAAAIVISLVISLTTTPMLCAHLDLHKQERKQ
                     GWLLNGAERAFEWSKRAYSSSLRWALGNPGTIMFILLVAVGLNFFLYYEVPKGFLPGQ
                     DTGQIRGGIRGDQSISFQLMEKKFVQFVNIIAKDPAVENVVGFTGGGGGGPRGGGLNS
                     GNVFIQLKPLAQRNNLSTELVIDRMRGKLSGVAGARLTLRSSGDFRAGGRQSLATYQY
                     TILGDTLSEVEEWAPKITAALENVPELEDVNSDREDRGLEVALSIDRATATRLGISMS
                     QIDSALYNAFGQRQVSTIYNDLNQYHVVMGVAPEFWQSPETLKDIYVSTSGGALSGTQ
                     ATGTVAGTTVISSATTQTAADVAADSLRNQQLNALTASARGGVSTGSAVSTRMSKMVP
                     LAAFSQFGPSTTPISINHQGPFVATTFSFNLPPGVSLGAATSAIQRTMAGLHVPISLH
                     GEFAGNARLFAQSINDEPLLILAAIVAVYIVLGVLYESYVHPLTILSTLPSAGVGAVM
                     ALLITGTDFNLLALIGVILLIGIVKKNAIMMIDFALAAERERGLSSRDAIFEACQMRF
                     RPIMMTTFAAILGALPLAIGFGSGAGFRQPLGISIVGGLVVSQLLTLYTTPVVYLYMD
                     RWRLRSKRRWNRFYSRILGDAAPGAGHVTGG"
BASE COUNT         1590 a         2497 c         2573 g         1477 t
ORIGIN      
        1 catcgcggtg tatgaagaac gagaggccgc gcgcggccta aagatggtat atgagccgcg
       61 ctatctgcgc ttcttccagg cgcgcttcga aaggctctga gcgaacttca tggacccggc
      121 atttcgcatc ggtgacgtta cgatccccgg ccgggtgctg accgcgccga tgagcggggt
      181 gtcggacctg cccttccggt gcgcggcctc gaagctcggc gcaccttatg tcgtgaccga
      241 aatggtcgcc tgcgagacgc tggccagcgg ccggccggat gtcgtgcggc gcgcggcggt
      301 gggtgagggt ctaccgttga tggtggttca aattgtgggg cgcgaagcgt gctggatcgc
      361 ccaaggcgcc gccatggcgg aagcctcggg cgcccagatc atcgacctca acatgggctg
      421 cccggccagg gaagtcaccg gcgggctttg cggctcggcg ctgatgcggg acctcgacca
      481 ggccgagcgc ctgatctccg cggcggtggg agcgaccagc cggccggtca ccttgaagat
      541 gcggctcggt tgggatgatg cctcccgcaa tgcgccgcag cttgcggcgc tcgccgagcg
      601 ctgcggggtg aaggcgatta ccgtccacgg ccgcacgcgg cagcaattct acaaggggcc
      661 gagcgattgg cgcgcggtgg cggaggtgaa agcggcaaca agcctgccgg tgatcgtcaa
      721 cggcgacgtg gtcgacgatg tttccgcccg cgaagcgctg aggcaatcgg gcgccgatgc
      781 cgtgatgatc gggcgaggcg cttgcggcag gccttggatc gccgccgcgg tcgagcgggc
      841 cttgcgcacg ggcggagcca tgatcgaacc cagccttgcg cagcgcctgg acatcgctct
      901 cgatcatttc cgcgacagtt tgcgcttcta cggcgacgct ctcggcttga agatattccg
      961 caaacatctg ggctggtacg tcgaaggggc agaccggccg cagggtgcgc aaacgcgccg
     1021 ggcggcaaag tcgcgcctgt gccggctgac gcaaccgggc gaagtggagg cgggtttgat
     1081 cgctctgtgg ggaacggcgc aacgaggaac actgcaatga ccaaggacga actgatggcg
     1141 tgggcgctcg ccaacggctg gcagatgatg ggcggctatc ccagcctgcc caaaccgcgt
     1201 cagcccgatg tggcgatcgt ccgcctggtg ctgaaagcga cggtggccag cctggaaatc
     1261 aggaagccca ccggcaaatg ggaaaaggtg gcgggcgaaa gctattccaa gatcgcggcc
     1321 gatcccgata acgggatgcc gagcgggctc ggtttcgcca ccatcaacgg cctctccacc
     1381 ctgatgcggg acaacaagga ccgcttggtg ttcgccaaat gaaaggggcg tagtccggcc
     1441 gcggccggaa accgcaaaac cccagtcgca cccggataaa aaccgtcgta ccatcgcgca
     1501 tccgactcgg aggcatattc catgacgaaa tccatccgaa ccacgcttct gctcggtgtt
     1561 ctggattggc tgatcccgtt tgcgatctcg ttcgcggttt tccctcttaa ggccctgaac
     1621 tactttctgt ttgaatccgt catgacgatc gcgatcgtct tcgccgccat tttgctcggc
     1681 gtcatttatt tcaaggacct gaaagacacg acggcgaatt tcgtccgcgc cggactcttg
     1741 ctggggctga tctggctcgc tatcaatctt gcggccgatc tgctgatctt cctgccgaaa
     1801 agccccatgc agattcctct gcctttgtac atgagtcaga tcggggtgaa gtatctgagc
     1861 attccgatca tcacggccgg tctgggctat ctgaaaaaac aagcaggaca ggcggccgga
     1921 taacgccgcc cccgcttaga gcgcttcccg gaaagtgggg ccgataggtc atgagcggaa
     1981 gcgaacgacg cgtattcggc ggttttcgga taagacgcgc gacgaaacaa atagctagag
     2041 caaatcatcg attccaataa acggtgattt gctctagaga ttgatccgat ttaattgaat
     2101 cggatcaatc tctagattcc ttgattttcg cgcaatctga cggcaaaccg cgtcggcacg
     2161 ctgctgctac cgaccacttt gccggattgc gctccagcgg ctgtcgttcg aattccgccc
     2221 gaaagtaccc gctgtcattc ccggccgagc aaagcgttgg ctttgcgggg ggtgcgggta
     2281 agtcgaaaac cgtccgttac agtttgtacg ccttcttcgg cagcttgtag gcgagatcga
     2341 cggcggtttc cgccgcttcg gtctcgtcca ggcggtgcgt cgccaccagt tcggccagat
     2401 aggcgctcat cacccgccgg tacagattgt ggcgggccgg gatcgagaag aaagcgcgcg
     2461 tgtcgtcgtt gaagccggcc gtgttgtaga agccggcggt ttcggtggtc tgccggaagt
     2521 aacgcatcat gccttcgggc gaatcgtgga accaccaagc cggccccagc ctgaggcagg
     2581 gatagtggcc ggccagcggc gccagttcgc gcgcgtaatt gctttcgtcg agggtgaaca
     2641 ggatgacggt gaggtccgcg cggttgccga cgcggtcgag caagggcttc agcgcatcca
     2701 catagctcgt gcgcatcggg atgtcggcgc cgcggtccgg cccgtagccc gcaaacaacg
     2761 tcttgttgtg gttgcggaag gcgcccggat gaatctgcat cgtcatgccg tcctcgcacg
     2821 acatcagcgc catttcggtc agcatctggg cgcggaacaa gtcggcctcg cccggcgcca
     2881 gcgtgccctt gagggcgcga tcgagcagcc cctggcattc gttcgccgga agatcggcgg
     2941 tcctggccgt cggatggcca tgatcggtgg ccgtggcgcc atgggtgcgg aagaaggcgc
     3001 gccggttggc cagcgccgcc agatagcctt tccaagtggc ggtattctcc ttggtcaagg
     3061 cgcccaattc ggcgacgttc gccgcgaagc cgcgggcatc gggatcgacc acgccgtccg
     3121 gccggaaggt ggtgacgacc ttgccgtccc attcggattg cagcttggtg tgatggagca
     3181 acggatcgtt ggcgccttcg gtcgtggcca aagcttcgat gttgaaacgc ttgaacaagg
     3241 cgcgcggacg aaactccggc ttcttcagcg cttcggtgat cgcgtcgtaa tagagatcgg
     3301 cggtggccgc ttccagccgc acctcaatcc cgaacaccgt cgcgaaggca tgatcgagcc
     3361 aggtccgcga gggcgtgccg cggaacagcc agtaattctc ggccagcagc cgccaactgg
     3421 cgcggcggtc cttttcggcg gtgccgcccg caggtgcaat gccgagctgg gcgagcgtaa
     3481 tcccctggct gtacagcatg cggtgcacat agtgatcggg ccacaggaac aggctcgtcg
     3541 catcctcgaa cggcgcatcg ttcgcgaacc aggaggggtc ggtgtggccg tgcgggctga
     3601 tgatcggcag cgtctcgaca tgtgcgaaca gccggcgcgc gatggttgcg ctaccactat
     3661 cggtcgggaa gaagcggtcg ggatgaaggc ggagtgcgga agtcatggga gaccctcgga
     3721 aaaggcggtg taacgtactc aagctgctat acgaatgtcc atcatagaaa cctgcccagg
     3781 agcgcgcggg gcggggcgag cgttgacaat gcccgctatc gtgcgcggcg aatgatcgcg
     3841 gagttcggcg tgacaagaat tgacccggta cggattatcc ggcgggcctt gggcctagcc
     3901 ccggccggac cgcaataact cgccatgggg cggctccggc atttcgcctt cgcctggctg
     3961 ttcgtcctca acggcctgct gttctgggcc tataccatcg cgcgcgggca tctcagccgc
     4021 gacctgctac ctacaaaagg agctgcccat ctgccgcgcg aactcctcaa ccatctcagg
     4081 ctaaggttcc ccgaggacaa ggacgccaag cgctacaatg cggagcagaa aaagcgtcat
     4141 cttcgtgccg gggccgctcg tggtgctgac gggcctgacc atgctgcgcg cggttccttc
     4201 gcctacgcaa atcacccgcc acgattgtgt cgaaggatgg agctgcatcg gcgagtggac
     4261 cggaacacct ctgagcctca tcctcgccgc cgccaaaccg aagacaaacg cgcggctatc
     4321 ttgtgttcca ttgcgccggc gacatggctc aggaagggag gcggcgcagt attattacga
     4381 gagcctggac ctcttggagg ccatctaccc cccagacaat tccggcttat gaattaaacc
     4441 gtcaggcgtt gccggttgaa cacggcgctc ccgtgcggct gcgcgccgaa cggcaactcg
     4501 gctacaaaat ggggaagttc ttgaatcgta tagaagttgc cgagagttat acccagatca
     4561 aaggcgggcg cggcggctat tgggaagacc aaggctacaa ctggtacgcc ggaatctagg
     4621 caagtcagaa agaacccttc gccccccttg tcggaagccc ttccacaccc gatattcggc
     4681 taagcattaa aagttaatga ttgattcgta gaatgcggag ctgcccccgg caggtttaaa
     4741 atatggcata tcgttcttgg tttctcgcgg tcgcattgct gagcgttctg actgcgggtt
     4801 gtgcgacgag caaccctgaa tcgctggctc aaaacgatcc ttacgaaccc accaaccggg
     4861 ccgtgttcaa agtgaatatg gcgatagaca acgccgtcgc caagccggtc gccaaattct
     4921 acaaccgtgc cctccccgtg cctgtgcgta acggcatcca caacttcctg accaatctca
     4981 acaagccggt cactttcggc aacgatgttc tccagggcga acccgttcgt gccagcgaaa
     5041 cgctggtacg cttcaccctg aacagcacgc tgggcatagc cggtctgttc gacgtcgcct
     5101 ccactttagg cgttcccgat cattccgagg atttcgggca gaccctgggc gtttggggcg
     5161 ccggcgaagg gccttatctg gtcctgccgt tcatcggccc cgacccgccg cgcgacctga
     5221 ccggcaacgt cgccgacatc ttcatggacc cgttcaccta catcaacttc catggctccg
     5281 acacttggta tgccgtgcgt acaggcgtgg ccgtgctgga tctgcgcgcg cgcaacgtgg
     5341 acacgctgga gcagatcgaa cggacgtcga tcgacctcta cgccacgacg cgcagcctct
     5401 atcgccagta ccgcaacgcc gaaatccgta acggcgcgcc cgagcctcag gacacctccg
     5461 acacgccgga attctaaccc ccggtcacgt ggccggcgcc gggagcggca tctcccagga
     5521 ttcgcgaata gaagcggttc cagcggcgct tggagcgcaa gcgccagcgg tccatgtaaa
     5581 gatacacgac gggcgtcgtg tagagcgtca gcaactggct gacgaccaac ccgccgacaa
     5641 tcgaaatgcc cagcggctgg cggaatcccg cgccgcttcc gaacccgatg gccagcggca
     5701 gcgcccccaa aatagcggcg aaggtcgtca tcatgatcgg gcggaagcgc atctggcagg
     5761 cctcgaaaat cgcgtcgcgc gaactcaggc cgcgctcgcg ctccgccgcc agcgcgaagt
     5821 cgatcatcat aatggcgttc ttcttcacga tgccgatcag aaggatgacg ccgataagcg
     5881 ccagaaggtt gaaatcggtc ccggtgatga gcaacgccat caccgccccg acgcccgccg
     5941 aaggcagggt cgagagaatc gtcaagggat gcacgtagct ttcataaagc acgccgagca
     6001 cgatatagac ggcgacgata gcggcgagga tcaagagcgg ctcgtcgttg atcgattggg
     6061 cgaacaacct ggcgttcccg gcgaattccc cgtgcaggga aatcggaaca tgcaggccgg
     6121 ccatcgtgcg ctggatcgcg ctggtggccg cgcccagcga tacgcccggc ggaagattga
     6181 aggaaaaagt cgtcgccacg aacggaccct gatggttgat cgatatcggc gtggtgcttg
     6241 gaccgaattg gctgaacgcc gccaagggaa ccatcttcga catgcgcgtc gagaccgccg
     6301 aacccgtcga aacgccgccg cgcgcgctgg cggtcagggc gttgagctgc tggttgcgca
     6361 acgaatcggc cgcgacatcc gcggcggttt gcgtcgtcgc gctggaaatc accgtcgtgc
     6421 cggcgacggt gcccgtcgcc tgcgtgcccg agagggcacc gcccgaggtg ctgacgtaga
     6481 tgtccttcag ggtttcggga ctctgccaga attccggcgc cacgcccatg acgacgtgat
     6541 actggttcag atcgttgtag atcgtggaaa cctgccgctg gccgaaggcg ttgtagagcg
     6601 cgctgtcgat ctgcgacatc gatatgccga gcctggtcgc ggtggcgcgg tcgatcgaca
     6661 acgccacctc gagcccgcgg tcttcgcggt cggaattcac atcttcaagt tcgggcacgt
     6721 tctccagcgc cgcggtgatc ttcggggccc attcctcgac ctcggacagc gtgtcgccga
     6781 gaatcgtata ttggtaggtt gccagactct ggcgtccgcc cgcgcggaaa tcgccgctgc
     6841 tgcgcagggt caagcgcgcg cccgccactc cgctgagctt gccgcgcatc cggtcgataa
     6901 ccagttcggt gctcagattg tttctttgcg ccagcggctt gagctggatg aagacgttgc
     6961 cggaattcag cccgccgccg cgcggcccac cgccgccgcc gccggtgaat ccgacgacgt
     7021 tctccacggc cggatccttg gcgatgatgt tgacgaactg cacgaacttt ttttccatca
     7081 actggaagga aatgctctgg tcgccgcgaa tgccgccacg aatctggccg gtgtcctggc
     7141 cgggcaggaa gcctttgggc acctcataat acaggaagaa attcagcccc accgccacga
     7201 gcaggatgaa catgatggtg cccggattgc ccagcgccca gcgaagcgac gagctgtaag
     7261 cgcgcttgct ccattcgaaa gcccgctcgg ccccgttgag caaccaacct tgcttgcgct
     7321 cctgcttgtg caggtcgaga tgggcgcaga gcatcggtgt cgtcgtcagg gaaataacca
     7381 gcgagatgac gatggcggcg gccagagtca ccgagaattc ctggaagaac ttaccgatga
     7441 ttccacccat cagcagaatg ggcagaaaga ccgcgatcaa ggacaggctc atcgccagga
     7501 ccgtgaagcc gacctcgcgc gcgcccttga gcgtcgcctc gaagcgcgac atgccctgct
     7561 cgatgtagcg ggtcacgttt tccagcacga cgatggcgtc gtcgacgacg aacccggtag
     7621 ccaccgtcag cgccatcagc gacagattgt cgagcgaata gccgagcagg tacatcaccg
     7681 tgaaggtacc gatcagcgac agcggaaccg caacgcacgg gatcagggcg gcacggccgt
     7741 tgcgcaggaa cagatagacg acgaacatca ccaacaggat ggcgatcacc atggtgattt
     7801 ccatgtcgtg cagcgaagcg cggatcgacg tggtctggtc gttggcgatg atcaggcgga
     7861 tcgaagacgg cagcgctttt tgaagccggg gcagctcggc cttcacattg tcgaccgtct
     7921 cgatgatatt ggcgcccggc tggcggaaca ggatcaccag aatggccggc ttgccgttcg
     7981 ccatgccgag attgcgcacg tcctcgacgc cttcatagac cttacctacg tccgacagcc
     8041 tgaccgccgc gccctcccgg taggccaaga ccaaagagcg gaaatcggac gcctcgcggg
     8101 cctgatcgtt gacataaatc tgatagcgta gcggccc
//
LOCUS       DASWPU010000456         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_22672,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000456 DASWPU010000000
VERSION     DASWPU010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22672"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..>2295
                     /locus_tag="VGC27_09730"
     CDS             274..>2295
                     /locus_tag="VGC27_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008282980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY0282890.1"
                     /translation="MIAEVGLFALILALFVAVVQAAVPLIGAARGDAAWMNLDRPAAF
                     AQFCLVSISFGALIWLHVTSDFSVLNVALNSHTDKPLLYKITGVWGNHEGSMLLWTFI
                     LVLYGLAVSVFGRDLPPGLRARALSVQGMISVGFLLFILLTSNPFARIDPAPLNGNGL
                     NPLLQDPGLAFHPPFLYLGYVGFSMAFSFAIAALIEGRVDAAWARWVRPWTLAAWCAL
                     TLGVAIGSWWSYYTLGWGGYWAWDPVENASLMPWLAGTALLHSAIVAEKRDAMKSWTI
                     FLAIVTFSLSLLGTFLVRSGILTSVHSFANDPSRGIFMLLLLVVVTGGSLILYAFRAP
                     SLKGGGLFSPVSREGGLLFNNVVLSTAAGTVLLGTLYPLFVDALGLSKVSVAAPYFNI
                     VMVPLMVPLILVMAVGPLLAWKRGDLPEALSRLKFALGAAVAAIAATLLAAGVSSRNL
                     WASLGLGLAVWLLAGSLTEWAGRVRLFRSPFAGSFRRALHLPRSSYGMTLSHMGLAVL
                     LIGVAGSLAWQTELLQVMRPGATAALAGYQIKFIGVEDNVRGPNYIASRARFIATKNG
                     RFVSELRPERRMFANPPQPLSTVAIHTNFVSDLYAVLGDPDGKGGHVVHIYHNPLVPW
                     LFFGAVMMVLGGMVSLTDRHHRIGAPLRRQAARIPMPTIKAEAPIAVASD"
BASE COUNT          331 a          729 c          725 g          510 t
ORIGIN      
        1 gatttcgtcc acaagggttt tcccgacaaa gcgcgcaggc aaccgccacg gcggaaaccc
       61 gcaaggaaag caaaagcgtc tagagtttga tccgatttaa ttgaatcgga tcaagctcta
      121 gattccttga ttttcgcgca atctgacggc aaaccgcttc acactttgcc ggattgcgct
      181 ctagtgtcgt ggtttcgaag ttcgcagcat ccgctatatc ggcttcgggc gaatttcgga
      241 atcgggacac cagggcatga cggatatggt cggatgattg ccgaagtcgg tctgtttgcc
      301 ctgatcctgg cgctcttcgt tgcggtggtt caggccgcgg ttccgctcat cggggccgcg
      361 cgtggcgacg cggcgtggat gaacctggac cggccggcgg ccttcgccca gttctgcctg
      421 gtgagcatct ctttcggcgc gctgatttgg ctgcacgtga ccagcgattt ctcggttctc
      481 aacgtcgcct tgaacagcca caccgacaag ccgctgctct acaagatcac cggcgtctgg
      541 ggcaatcacg aaggctccat gctgctgtgg accttcatcc tcgtgctgta cggcctggcg
      601 gtatcggtgt tcgggcgcga cctgccgccg ggactgcgcg cgcgcgcctt gtcggttcag
      661 gggatgatca gcgtcgggtt tcttctgttc atcctgctga cctccaaccc gtttgcgcgc
      721 atcgatccgg cgccgctgaa cggcaacggc ctcaatccgc tgttgcagga ccccggtctc
      781 gccttccatc cgccgtttct ctatctcggc tatgtcggct tctcgatggc gttttcgttc
      841 gccatcgccg cgctgatcga agggcgggtg gacgccgcct gggcgcgctg ggtgcggcca
      901 tggacgctgg cggcgtggtg cgcgctgacg ctgggcgtcg ccattgggtc gtggtggtct
      961 tactacacgc tgggctgggg cggctattgg gcgtgggacc cggtggagaa cgcctcgctc
     1021 atgccttggc tggccggaac ggcgctgctg cactccgcca tcgtggccga aaagcgcgac
     1081 gcgatgaagt cgtggacaat cttcctggcg atcgtcacct tctccttgtc cctgctcggc
     1141 accttcctgg tgcgctccgg catcctgacc tcggtgcaca gtttcgccaa cgatccctcg
     1201 cgcgggatat tcatgctgct tcttctggtc gtggtcaccg gcggttcgtt gatcctgtat
     1261 gccttccgcg cgccgtcgct caaaggcggc ggcttgttct cgccggtgtc gcgcgagggc
     1321 ggtttactgt tcaacaatgt cgtcctgagc accgccgcag gcacggtcct gctcggcacg
     1381 ctgtacccgc tgttcgtcga tgcgctcggc ctcagcaagg tttcggtcgc ggcaccctat
     1441 ttcaacatcg tcatggtccc gctaatggtt ccgctgatcc tggtcatggc ggtggggccg
     1501 ctgctggcgt ggaagcgcgg agatttgccg gaggcgctgt cgcgcctgaa attcgccttg
     1561 ggcgcggcgg tcgcggccat cgccgcgacc ttacttgccg ccggcgtgtc gtcgcgaaac
     1621 ctttgggcgt cgctggggct tgggctggcg gtctggcttc tggccggcag cctgacggaa
     1681 tgggccgggc gggtgcgtct gttccgaagc ccgttcgccg gctctttccg gcgcgcgctc
     1741 catctgcccc gttccagcta cggcatgacg ctcagccata tgggattggc ggtgctgctg
     1801 atcggcgtcg ccggatcgct ggcatggcag actgagcttc tacaggtcat gcgtcccggc
     1861 gccaccgccg ccctggccgg ctatcaaatc aagttcatcg gcgtcgaaga caatgtcaga
     1921 ggtcccaatt acatcgcgtc gcgcgcgcgc ttcatcgcca cgaagaacgg ccgcttcgtt
     1981 tccgaactgc ggccggagcg gcgcatgttc gccaatccgc cgcagccttt gtccacggtc
     2041 gccatccata ccaatttcgt cagcgacctg tacgcggtcc tgggcgatcc cgacggcaag
     2101 ggcggccatg tcgtccacat ctatcataac ccgctggtgc cgtggctgtt cttcggcgcc
     2161 gtgatgatgg tcttgggcgg catggtgtcg ttgaccgaca ggcatcatcg catcggcgcg
     2221 cctttgcgca gacaagccgc aagaatcccg atgccgacta taaaggcgga ggcgccaatc
     2281 gccgttgcat cagac
//
LOCUS       DASWPU010000457         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_46956,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000457 DASWPU010000000
VERSION     DASWPU010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46956"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VGC27_09735"
     CDS             <1..509
                     /locus_tag="VGC27_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282891.1"
                     /translation="KLPRDRPLLIVDADEVILRFTDGFDRFLRLRGYYLDLTSYRLYG
                     NVKSLTALAQMASVVVLTNISEAQAPARRRNLLSLGLDYPLVVNEGLKGPAVKNLAGH
                     VCAACFFIDDVPQHLASAEEMAPEVVRIHLVGDTRLAALLPLPFHAHYHTEDWEAAEI
                     FIKARLGK"
     gene            531..1457
                     /locus_tag="VGC27_09740"
     CDS             531..1457
                     /locus_tag="VGC27_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0282892.1"
                     /translation="MRFGIDLGGTKIEIMALGEKGKEQLRQRISTPRDKYENVVRAIS
                     DLVRDAEVKLGCRGSVGVAIPGAISRKTGLVKNANSTCLIGHPLDKDLSVALRREVRV
                     ENDANCFALSEATDGAGAGYGTVFGIIAGTGVGGGICVNGKVLPGAHGIAGEWGHNPL
                     PAPGRQETEDARRCYCGRYGCIESWCSGPSLASLYEARTGHKLSAPEIVEAAGRGDGP
                     AAAEMEAFYDRLARSIAGVVCILDPDVIVMGGGLSNIEALYTELPPRVERYAFTPEGP
                     TNIVKNVHGDSSGVRGAAWLWREEEAATGLPK"
     gene            complement(1464..>2277)
                     /gene="aguB"
                     /locus_tag="VGC27_09745"
     CDS             complement(1464..>2277)
                     /gene="aguB"
                     /locus_tag="VGC27_09745"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR03381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEY0282893.1"
                     /translation="ANVAKAKELVRAAAGKGANVVLLQELFETPYFCQDQSPEHFKLA
                     APVEGNPLIAGMAELAEALNVVLPVSFFEKAGHAFFNALAMIDADGKVLGVYRKSHIP
                     DGPGYQEKYYFTPGDTGFKVWRTRFGVLGAGICWDQWFPETARAMALMGAEALLYPTA
                     IGSEPQAPLLDSRDHWRRTMQGHAAANCMPLIASNRIGTEKGLAGELTFYGSSFIADA
                     TGAIAAELGRSEESFATSTFDLDEIAKTRASWGLFRDRRPDLYGALTSHDGT"
BASE COUNT          419 a          715 c          729 g          414 t
ORIGIN      
        1 caaaactccc ccgcgaccgg ccgttgctga tcgtcgacgc cgacgaggtg atcctgcgtt
       61 ttaccgacgg tttcgaccgg ttccttcggc tgcgcggata ctatctcgac ctgaccagct
      121 atcgcctcta tggcaacgtc aagtcgctga ccgccttggc gcaaatggcg agcgtcgtgg
      181 tgctgaccaa catctccgag gcacaagctc cggcgcgccg gcgcaacctt ttgtcgctgg
      241 gtctcgacta cccacttgtc gtaaacgaag gcctgaaggg accggcggtc aagaacctcg
      301 cgggacatgt gtgtgcggca tgtttcttta tcgacgacgt gccgcaacat ttggcgtccg
      361 cagaagaaat ggcacccgaa gtggtaagaa ttcatctcgt tggcgacacc agactggcgg
      421 cgttgttgcc gctgccgttt catgcccatt atcataccga ggattgggag gcggccgaaa
      481 tatttatcaa ggcgcgcctg ggaaaataac cggatattcg gaaacaggcg atgcgctttg
      541 gtattgatct cggcggcacg aaaatcgaaa tcatggctct tggcgaaaag ggcaaagaac
      601 agcttcggca gcggatttca acgccgcgcg acaagtacga aaacgtcgtc cgcgccatca
      661 gcgacctggt ccgcgacgcc gaagtcaagc tcggctgccg cggcagcgtg ggcgtcgcta
      721 taccgggcgc catcagccgc aagaccggcc tggtcaagaa tgccaactcc acctgtctga
      781 tcggccatcc tctcgacaag gacctgagcg ttgcgcttcg gcgcgaagtc cgcgtcgaga
      841 acgacgccaa ctgctttgcg ctttcggaag cgacggatgg agcgggcgca ggctatggca
      901 cggtgttcgg catcattgcc gggacgggtg tcggcggcgg catctgcgtg aacgggaaag
      961 tcctgcccgg cgcgcacggc atcgccggtg aatggggcca caaccccctg cccgcgcccg
     1021 gacgccagga aaccgaagac gcgcggcgtt gctattgcgg gcgttacggc tgcatcgaga
     1081 gctggtgcag cggcccctcg ctggcttcgc tttacgaggc gcgcaccggg cacaagctct
     1141 cggcgccgga gatcgtcgaa gcggccggcc gcggcgacgg ccccgctgca gccgagatgg
     1201 aagcctttta cgaccgcttg gcgcgctcga tcgccggcgt ggtctgtatt ctggacccgg
     1261 atgtcatcgt catgggcggg ggattgtcga acatcgaggc gctttatacg gaactgccgc
     1321 cgcgggtgga gcgctacgcc ttcacgcctg aagggccgac caacatcgtg aagaacgtgc
     1381 atggcgattc cagcggcgtg cgcggcgccg cctggttgtg gcgcgaggaa gaagccgcga
     1441 ccggcctgcc gaaatagcgg atgctatgtg ccgtcatggc tcgtcagcgc gccatagagg
     1501 tccggacggc ggtcgcggaa caggccccac gatgcgcgag tctttgcgat ctcgtcgagg
     1561 tcgaacgtcg acgtcgcaaa gctttcctcg ctgcgcccca gctcagctgc gatagcgccg
     1621 gtggcgtcgg cgataaacga cgaaccgtag aaggtcagtt ctcccgccag ccccttctcg
     1681 gtgccgatgc ggttcgatgc gatcagcggc atgcagttgg cggcggcatg gccttgcatc
     1741 gtgcgccgcc aatggtcgcg gctgtcgagc agcggcgcct gtggttcgct gccgatggcg
     1801 gtcggataga gcagcgcttc cgcgcccatc agcgccatcg cccgcgcggt ttcggggaac
     1861 cactggtccc agcagatgcc cgcgcccagc acgccgaagc gcgtgcgcca gaccttgaag
     1921 ccggtatcgc cgggcgtgaa atagtatttc tcctgataac ccggtccgtc cggaatgtgg
     1981 gatttgcgat agacgccgag cactttgccg tccgcgtcga tcatggccag cgcgttgaaa
     2041 aaagcgtgcc cggccttctc gaaaaagctg accggcaata cgacattcag cgcttcggca
     2101 agctcggcca tcccggcgat cagcgggttg ccttccacgg gcgccgccag cttgaaatgc
     2161 tccggcgact ggtcctggca gaaatagggc gtctcgaaca attcctgcaa cagcacgaca
     2221 ttggcgcctt tacccgccgc cgcgcgcacc aattccttcg ccttggcgac gttggct
//
LOCUS       DASWPU010000458         3957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_83740,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000458 DASWPU010000000
VERSION     DASWPU010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83740"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VGC27_09750"
     CDS             <1..545
                     /locus_tag="VGC27_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282894.1"
                     /translation="DAAGLRAALAESLGPGALEGRAAVIWGAGGAARAAVCALSDLGV
                     AEIRILNRDKARAESLAAALRSKVAAKLACGGYEDWSGAGKDAALLVNATSAGLKGAP
                     SLALPLEVLPAGAAVFDAVYNPLETGLLAAAKARGLVAIDGLGMLMHQAVPAFAAFFG
                     VEPKITAALRNMLEEALGRG"
     gene            538..1161
                     /gene="coaE"
                     /locus_tag="VGC27_09755"
     CDS             538..1161
                     /gene="coaE"
                     /locus_tag="VGC27_09755"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY0282895.1"
                     /translation="MAERPFMVGLTGSIGMGKSETAKMFARLGLPVYDADAAVHALYE
                     PGGAAVAEIAQAFPGSVKNGRVDRGELARQLGADRAAFARLEAIVHPLTAAAERAFIE
                     RARAEGAQLVVLDIPLLFETGGKARMDAVVVVSASPQVQRARVLAREGMSEERLDGLL
                     ARQMPDAEKRAQADFVVETDQGLDHAFSQVKKIVAQLSERRAKQGTG"
     gene            1181..1870
                     /gene="dnaQ"
                     /locus_tag="VGC27_09760"
     CDS             1181..1870
                     /gene="dnaQ"
                     /locus_tag="VGC27_09760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY0282896.1"
                     /translation="MVLDTETTGLDPSDGHRIIEIGCVELVDHFPTGRTYQTYLNPER
                     EMPAEAERVHGIGSAFLADKPRFAEIVEAFLEFLGQAPLVIHNAGFDLKFLNAELERI
                     SAKPIPASRAIDTIEIAKNKLPGARYSLDELCKRFGIDLSAREKHGALLDAELTAQVY
                     LELIGGRQKRLLLAPADETSAGPADDSRSARARPQPLPPRLSDDELSRHADFVSRELD
                     DAVWTWGRETA"
     gene            complement(1867..2370)
                     /gene="secB"
                     /locus_tag="VGC27_09765"
     CDS             complement(1867..2370)
                     /gene="secB"
                     /locus_tag="VGC27_09765"
                     /inference="COORDINATES: protein motif:HMM:NF004392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY0282897.1"
                     /translation="MSGEGTAPETGSENGMDTQAGRIQVVGQYIKDLSFENPAAPGNL
                     TTRPQIDLNVDLQARQLEREHYEVELRLRVSATCEDKPAFLLELVYAGLFQLQNIPEE
                     VRNQILLIEAPHILFPFARRIVADMVRDGGMPPLMIEPIDFAALYRAKAAEMQQARTA
                     NPGQLDA"
     gene            2580..3263
                     /locus_tag="VGC27_09770"
     CDS             2580..3263
                     /locus_tag="VGC27_09770"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEY0282898.1"
                     /translation="MANIQLIDIFLIAMVAGVILFRLYTVLGRRTGHERPPQENYRLS
                     GNTPARGGDDAAIATGEGPTMARPALTARPADPVAGGLHDIQQADHGFDKDRFVVGAR
                     AAYEMIVTAFANNDRAGLRPLLSEEVYTAFEAAMRGREKRGEKVAFTFVGFKEVKIVA
                     AALKGRMAEVTLMFDAQFISATTDAKGALVDGDAKTVRSVIDTWTFSRDARARDPNWT
                     LVATSGEAA"
     gene            3355..>3957
                     /locus_tag="VGC27_09775"
     CDS             3355..>3957
                     /locus_tag="VGC27_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEY0282899.1"
                     /translation="MRAPKPGPLRLTKVSFAALPGWQKSDPGAALAAFRRSCAALERR
                     DSSASMGGAFYGGSVAEWRAPCRAAGSASPAQAHAFFESWFVPLRISAGAATDGLFTG
                     YYEPDLSASRTRHGAFQTPIYGVPDDLVSVDLGAFRPQLRGERIAGKVEGGRLVPYAS
                     RAQIDAAGLPQARILFYSDDPIAVFFLHIQGSGRVRFDDGS"
BASE COUNT          679 a         1219 c         1332 g          727 t
ORIGIN      
        1 ccgacgcggc gggcttacgc gcagccctgg ccgaaagtct cggcccaggc gctcttgaag
       61 gccgtgcggc ggtaatctgg ggcgcgggag gagcggcgcg cgctgccgtc tgcgcacttt
      121 cggatttggg ggttgcggag atccggattc tcaatcgcga taaagcgcgc gcggaaagcc
      181 tggcggcggc gttgcgttcg aaagtggctg ccaaactcgc ctgcggcggc tacgaagatt
      241 ggagcggggc ggggaaagat gccgcgttgc tcgtcaatgc gaccagcgcc ggcctgaagg
      301 gcgcgccctc cctcgcgctg ccgctcgagg ttctgcctgc cggcgcggcg gtgttcgatg
      361 cggtgtacaa tccgctggaa accggacttt tggcggcggc aaaggcgcgc ggtctggttg
      421 ccatcgacgg actgggcatg ctgatgcatc aggcggtgcc ggcgtttgcg gccttcttcg
      481 gggtcgaacc gaaaatcacg gcggcgctga gaaatatgct ggaagaggct ttgggccgtg
      541 gctgagcgcc ctttcatggt cggcctgacc ggctcgatcg gcatgggcaa gagcgaaacg
      601 gcaaagatgt tcgcaaggct cggcctgccg gtttacgatg ccgatgccgc ggtccacgcg
      661 ctttacgaac cgggcggggc ggcggtggcc gagatcgcgc aggcttttcc gggttcggtc
      721 aagaatggcc gggtcgatcg cggcgaactg gcgcgtcagc tgggcgcgga ccgggccgcc
      781 ttcgccaggc tggaggccat cgtccatcct ttgacggcgg ccgccgagcg cgccttcatc
      841 gaacgggcaa gggccgaagg agcgcagctc gtcgttctgg atattccgct cctgttcgaa
      901 accggcggca aggcgcggat ggatgccgtg gtggtcgtct ccgcttcacc ccaggtgcag
      961 cgcgcccgcg tactggcgcg cgaaggcatg agcgaggaaa ggctggacgg cctgctcgcc
     1021 cgccagatgc ccgacgccga aaagcgtgca caagccgatt tcgtggttga aacggaccaa
     1081 ggcctcgatc acgctttcag ccaagtgaaa aaaatcgtgg cgcagctgag cgaacgccgc
     1141 gccaagcagg ggaccgggta agctgcaaaa cattcgtgaa atcgttctcg atactgagac
     1201 caccggactc gatccttcgg acggtcaccg gattatcgaa atcggttgcg tcgagctggt
     1261 cgatcatttt cccaccgggc gcacctatca gacctatctc aatcccgagc gcgagatgcc
     1321 ggcggaagcg gaacgcgtgc atgggatcgg cagcgcgttt ctcgcagata agccgcgctt
     1381 cgccgagatc gtggaggcgt tcctggaatt tctgggccag gcgccgctgg tgatccacaa
     1441 tgcgggcttc gacctgaaat tcctcaacgc cgagcttgag cggatctcgg caaaaccgat
     1501 accggcctcg cgcgccatcg acacgatcga aatcgccaag aacaaactcc cgggggcgcg
     1561 ctattcgctc gacgagttgt gcaagcgctt cgggatcgac ctgtcggccc gcgagaagca
     1621 cggcgctttg ctcgatgccg agctgacggc gcaggtctat ctcgaactga tcggcgggcg
     1681 gcagaagcgg ctgctgctgg ccccggcaga cgaaacctcc gccggcccgg cggacgatag
     1741 ccgttcggcg cgggcaagac cccagcctct accgccgcgc ctgagcgatg acgaactgag
     1801 cagacatgcc gatttcgtca gccgtgaact tgatgatgca gtgtggacct ggggccgaga
     1861 gacagcctaa gcgtccagtt gaccgggatt ggccgtgcgc gcctgctgca tttccgccgc
     1921 cttggcgcga tagagcgccg caaaatcgat cggctcgatc atcaagggcg gcatgcctcc
     1981 gtcgcgcacc atgtccgcga cgatgcgccg cgcgaacggg aacaggatgt gcggggcctc
     2041 gatcagaaga atctgattgc ggacctcttc ggggatgttc tgcaactgga agaggccggc
     2101 atagaccagt tcgagcagaa aagccggctt gtcctcgcag gtggccgaga cgcgcagcct
     2161 cagttcgacc tcgtaatgct cgcgttcgag ttggcgtgcc tgcaggtcca cgttcaggtc
     2221 gatctggggg cgggtggtca ggttgcccgg agcggccgga ttctcgaatg aaaggtcctt
     2281 aatatattgg cccacgacct ggatgcggcc ggcctgtgtg tccattccgt tttcgctgcc
     2341 cgtttccggt gccgttcctt cgccgctcat tgctgtaaac ccttgggaaa atgcctgcgg
     2401 gcgctagcac ggaacctgca ttcatgacaa gcaagcaacc tgcaaacgcc ccggaaacca
     2461 tgctttgagc gtcgtttttg tagggatatt tgaagggata taagctggac cctccgggcg
     2521 gctggcgtta gggtgggacc tcccgcgtgc cacgatgccg cggagaaagc actcacagca
     2581 tggcgaacat acagttgatc gatatttttc tgatcgcaat ggtagcaggc gtcatccttt
     2641 ttcgccttta caccgtcttg gggcggcgga cggggcatga aaggccgccg caggaaaatt
     2701 accggctctc cggcaatacc ccggcgcggg gcggcgacga cgccgccatt gcgaccggcg
     2761 aaggcccaac tatggcgcgc cccgccttga ccgcccgtcc ggccgatccg gtggccggcg
     2821 ggctccacga tatccagcag gccgaccacg gcttcgacaa ggatcgtttc gtcgtcggcg
     2881 cccgcgcggc ctatgagatg atcgtgaccg cttttgccaa caatgatcgt gccggcttgc
     2941 ggccgcttct cagcgaggag gtttacaccg ccttcgaggc ggcgatgcgc gggcgcgaaa
     3001 agcgcggcga aaaagtggcg ttcactttcg tgggcttcaa agaggtgaag atcgtcgcgg
     3061 ccgcactcaa ggggcgcatg gccgaggtca cgctgatgtt cgatgcgcaa ttcatctcgg
     3121 cgaccacgga cgcgaagggt gcgctggtcg acggcgacgc caaaacggtc cgcagcgtga
     3181 tcgacacctg gacgttctcg cgcgacgcgc gcgcgcgcga tcccaattgg acgctggttg
     3241 cgacgtccgg cgaagccgcc tgacgcgtcc gtggcggccc gcttccgact tttagcggcg
     3301 gtttttctcg gccttctcat gctcgccctt gttgcgggcg gaatttggtg ggccttgcgc
     3361 gcgccaaaac ccggcccgtt gcggctgacc aaagtctcct tcgccgcgct acccggctgg
     3421 cagaagagcg atccgggcgc ggctcttgcg gcctttaggc gatcttgcgc ggcgttggag
     3481 cgccgggact cttcggcaag catgggcggc gccttctatg ggggaagcgt tgcggagtgg
     3541 cgcgcgccct gccgcgcggc cgggtccgca agcccggccc aggcgcatgc attcttcgaa
     3601 tcttggtttg tgccgctgcg gatcagcgcc ggcgcggcga cggacgggct gttcaccggc
     3661 tattacgaac cggacctgtc tgccagccgc acccgtcacg gcgcgtttca aacgccgatc
     3721 tatggagtgc ccgacgatct ggtgagtgtc gatctcggcg cctttcgtcc gcagttgcgc
     3781 ggcgaacgca ttgccgggaa agtggagggt ggccgcctgg ttccttatgc cagccgcgcc
     3841 caaatcgatg ccgcaggtct gccccaggcg cgtattctgt tttatagcga tgacccgatc
     3901 gcggttttct tcctgcacat ccagggctcc ggccgcgtgc gcttcgacga cggcagc
//
LOCUS       DASWPU010000459         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_34970,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000459 DASWPU010000000
VERSION     DASWPU010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34970"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /gene="queA"
                     /locus_tag="VGC27_09780"
     CDS             <1..754
                     /gene="queA"
                     /locus_tag="VGC27_09780"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY0282900.1"
                     /translation="ARKLAPGDDLLLGEALRAKVTARGEGGEVELRFELAGVMLDAAI
                     AREGEMPLPPYIAGKRKPDERDATDYQTIFAAEPGSVAAPTAGLHFTPELLARLGGMG
                     VGRETVTLHVGAGTFLPVTAEDTEQHRMHPEWAMLPAAAATRLNAMRAAGGRIAAVGT
                     TSLRTLESAADESGRLQAFNGDTRLFIVPGYRFKTAEILLTNFHLPRSTLFMLVCAFA
                     GTEAMKRAYAEAIREHYRFYSYGDACLLFRPA"
     gene            751..1887
                     /gene="tgt"
                     /locus_tag="VGC27_09785"
     CDS             751..1887
                     /gene="tgt"
                     /locus_tag="VGC27_09785"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY0282901.1"
                     /translation="MTSAFSFEVSATDAAARCGIIRTPRGEIRTPAFMPVGTAGSVKA
                     MLPESVRETGADIILGNTYHLMLRPGAERVARLGGLHKFMGWERPILTDSGGFQVMSL
                     AKLRKITEEGVHFRSHIDGAEEFLTPERSMDIQRLLGSDIQMVLDECPALPASEETIE
                     KSLALSMRWAKRSKAAFGEQPGRACFGIVQGGTVTHLRVRSAEDLRAIGFDGYAVGGL
                     AVGEGQAAMFETLEFTLPHLPADRPRYLMGVGKPDDIAGAVLRGIDMFDCVLPTRSGR
                     NGQAFTWEGTLNLRNARHAEDAEPIDAACRCPACRRFSRAYLHHAVKANEIIASMLLT
                     WHNLTFYQDLMAGLRAAIGQGALASFAGGFLARYLARGEDAEKS"
     gene            1985..2059
                     /locus_tag="VGC27_09790"
     tRNA            1985..2059
                     /locus_tag="VGC27_09790"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2017..2019,aa:Lys,seq:ttt)
BASE COUNT          347 a          698 c          725 g          347 t
ORIGIN      
        1 ggccaggaag ctggcgcccg gcgacgatct gctgctgggc gaggcgctcc gggcgaaggt
       61 gaccgcgcgc ggcgagggcg gggaggtgga gctacgcttc gagctcgctg gagtgatgct
      121 cgacgccgcc atcgcgcggg aaggcgagat gccgctgccg ccctatatcg cgggcaagcg
      181 caagccggat gagcgcgatg caaccgatta tcaaacgatc ttcgccgccg aaccgggatc
      241 ggtggccgcc ccgaccgcgg gcctgcattt cacgccggag cttcttgcgc gccttggcgg
      301 aatgggcgtc gggcgcgaaa cggtgaccct gcatgtgggc gccggaactt tcctgccggt
      361 cacggccgag gacaccgagc agcaccgcat gcacccggaa tgggccatgt tgcccgcagc
      421 cgcggccacc cgcctcaacg cgatgcgcgc ggccgggggc cggatcgcgg cggtgggcac
      481 cacctcgctt aggacgctgg aatcggcggc ggacgaaagc ggccggctgc aggccttcaa
      541 cggcgatacg cggcttttca tcgtgccggg ttatcgattc aagaccgcgg aaatcctgct
      601 gaccaatttc catctgccgc ggtcaacctt gttcatgctg gtctgcgcct tcgccggaac
      661 cgaagcgatg aagcgggcct atgccgaagc catcagggaa cattatcgtt tctattccta
      721 tggcgacgcc tgcctgctat tccggccggc atgacgagcg cattttcctt cgaggtttcg
      781 gcgacggacg cggccgcccg gtgcggcatc atccgcacgc cgcgcggcga gatccgcacg
      841 cccgccttca tgccggtcgg cacggcgggc agcgtcaagg cgatgctgcc cgagagcgtg
      901 cgcgagaccg gcgcggacat catcctcggc aacacctacc acctgatgct gcggccgggg
      961 gcggagcgcg tcgcgcggct gggcgggctg cacaaattca tgggctggga acgcccgatc
     1021 ctgaccgact cgggcggctt ccaggtgatg tcgcttgcca agctgcgcaa gatcacggag
     1081 gagggggtcc acttccgttc gcacatcgac ggcgcggagg aatttctgac gcccgaacgg
     1141 tcgatggata tccagcgcct gctcggctcc gacatccaga tggtgctgga cgaatgcccg
     1201 gcccttccgg ccagcgagga aacaatcgag aaatcgctgg cgctgtccat gcgttgggcc
     1261 aagcgctcca aggccgcttt cggcgagcag ccgggccggg cctgcttcgg gatcgtccag
     1321 ggaggaacgg ttacgcatct gcgcgtgcgc tctgcggagg acctccgggc gatcggcttc
     1381 gacggttacg ccgtcggcgg cttggcggtg ggcgagggcc aagcggcgat gttcgagacg
     1441 ctggagttca ccctgccgca tctgcctgcc gacaggccgc gctatctgat gggggtgggc
     1501 aagccggacg acatcgcggg cgccgtgctg cgcggcatcg acatgttcga ttgcgtgctg
     1561 ccgacgcgct ccggacgcaa cggccaagcc ttcacctggg agggcacgct caatctgcgc
     1621 aacgcccgcc acgccgagga tgccgagccg atcgatgcgg cgtgccgctg cccggcctgc
     1681 cgccggttca gccgggccta tctgcaccat gccgtgaaag ccaacgagat catcgcctcg
     1741 atgctgctga cctggcacaa tctgaccttc tatcaggacc tcatggcggg gctgcgcgcg
     1801 gcgatcggac aaggcgctct cgcctccttc gccggaggtt ttctcgcgcg gtatctcgcc
     1861 aggggcgagg acgcggaaaa gtcctgaatt ccgcagattt tcggggggca aagcccggtt
     1921 gacccgcccg gcccctcccc atatggtccg cccgccccgc ctgcgcggga aaacgccctg
     1981 gagcgagccc gtagctcagg ggtagagcat ttgactttta atcaaagggt cgacggttag
     2041 atcccgtccg ggctcgccaa tcttccgtaa gggggaattc tcagcgccag tgaaactgca
     2101 gcagcttgcg cgccggc
//
LOCUS       DASWPU010000460         3956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_41261,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000460 DASWPU010000000
VERSION     DASWPU010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41261"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="VGC27_09795"
     CDS             complement(<1..1651)
                     /locus_tag="VGC27_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0282902.1"
                     /translation="MPPRQILLRLARDYLSRHWSVFALAITCMLITSAASASIPAFLD
                     FTIKHIFIERQADLLLPVALAVVVIMMLRAASWYGQKTLIDTLGERVVAASQRDMFDS
                     LIRRDLASLNAVHSGQFISNFLYDATLMRDAITQGTAAIAMEAVQLVFYACAMFYYDW
                     QLALISIVVLPGVAWAMNKIGVATRRASLRGLEETGGLSTALSEVLDGRRIIKAYGLE
                     SYVAERAHARLAKRMATLFKIVRARAAAVPSTDVFAGLVIAATLFYAGYQSLNGQLEL
                     NNFAAFIAAMLLAQQPVRNLSQLWPVASAGFAATNRVFGVIDAKPEIVDAPGAKTLVL
                     KQAPQGGAVKLKNVSFAYLADAQAAAVDRVNIDIAPGKKVALVGPSGAGKTTVFNLLL
                     RFYDVDSGSIEIDGRDIRGVTLASLRRNIALVTQDPILFDESVADNIALGKPGAGRAD
                     IERAARDADAHEFIMQLPDGYDTRVGEGGLKLSGGQRQRVAIARAMLRDAPILLLDEA
                     TSALDAESERHVQHALTRLMKDRTTIVIAHRLSTVVDADKLY"
     gene            complement(1904..2692)
                     /locus_tag="VGC27_09800"
     CDS             complement(1904..2692)
                     /locus_tag="VGC27_09800"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY0282903.1"
                     /translation="MPELDDAADTALLEDAVREAGVIARSFLTGQNKIRSKTDGSPVS
                     EADLAVDRFLKERLRPARPDYAWLSEETEDDPARLAARRVFIVDPIDGTVAFLKGRPH
                     FTISAAVVEGEIPVAASVYNPLTEECFTAARGAGARLNGEPIRVSSRESLVGCHMLAS
                     KTTLNDPCWGGSAWPPMAVETPNSIAYRIALVACGAADAAMTLAGTHDWDLAAADLIV
                     REAGGLISSHRGTVPRYNRADTTQPSVVAAGRALHEVILARVKA"
     gene            complement(2679..>3956)
                     /locus_tag="VGC27_09805"
     CDS             complement(2679..>3956)
                     /locus_tag="VGC27_09805"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY0282904.1"
                     /translation="ADAADALFVENVSASVSYRLGKLEDVERAESSDVGLRVFVGQRI
                     AFVSSTDFSKEGVTALPERAVAMARLAPEDKFACLAPRERLATSLPNLDIEDPAEPPA
                     DTLVERARTVEEAAMAVPGITNSEGGGASFGRAAIALATSEGFYGRYAGTHHSIGVAV
                     LAGEGTAMERDYESVSARHASDLEAAETVGRRAGERTVARLGPRKAKSQAVPVVFDPR
                     ESAGLLGHLTGAISGAAIARGVSFLKDRLGQEIFAPSITIVDDPHRLRGLRSKPFDGE
                     GVANAPTTVVENGLLKTWLLDCASAKQLGLTTTGHAARATSGPPHPASTNFHMLPGAL
                     SPAELMADIEEGFYVTELMGMGVNTVTGDYSRGASGFWIEKGKIAYPVSEVTIAGNLK
                     DMYRALTPANDLVFRYGVNAPTCRIEGMTVAGA"
BASE COUNT          634 a         1289 c         1350 g          683 t
ORIGIN      
        1 cgtaaagctt gtccgcgtcg accaccgtac tcagccggtg ggcgatcacg atggtggtgc
       61 ggtccttcat cagccgcgtc agcgcgtgtt gcacatggcg ctcgctctcg gcgtcgagcg
      121 ccgaggttgc ttcgtccagc agcaggatcg gcgcgtcgcg cagcatggcg cgggcgatgg
      181 cgacgcgctg gcgctgcccg ccggacagtt tcaatccgcc ttcgccgacg cgggtgtcgt
      241 agccgtcggg cagttgcatg atgaactcgt gcgcgtcggc gtcgcgcgcg gcgcgttcga
      301 tatccgcgcg gccggcgccc ggcttgccca gcgcgatgtt gtcggcgacg ctctcgtcga
      361 acaggatcgg gtcctgcgtc accagggcga tgttgcggcg gagcgaagcg agcgtgacgc
      421 cgcggatatc gcggccgtcg atctcgatgc tgccggaatc gacgtcgtaa aagcgcagca
      481 gcaggttgaa caccgtcgtt ttgccggcgc ccgaggggcc gaccagcgcc actttctttc
      541 ccggtgcgat atcgatgttc acgcggtcga cggccgccgc ttgcgcatcg gccagatagg
      601 cgaacgacac gttcttcagc ttcaccgcgc cgccttgcgg ggcttgcttc agcaccaggg
      661 tttttgcgcc gggggcatcg acgatctccg gtttggcgtc gatgacgccg aacacgcggt
      721 tggtggccgc gaagcccgcg gacgccaccg gccaaagctg gctcagattg cgcaccggct
      781 gttgggccag aagcatcgcg gcgatgaagg cggcgaaatt gttgagttcg agctggccgt
      841 tcagcgactg gtatcccgcg tagaacagcg tggcggcgat caccagcccg gcgaacacgt
      901 cggtggaggg caccgcggcc gcgcgcgcgc gcacgatctt gaacaaagtc gccatccgct
      961 ttgccagcct ggcatgggcg cgctcggcca cgtaactttc cagcccatag gctttgatga
     1021 tgcgccgtcc gtccagcact tccgacagcg cggtcgacag cccgccggtt tcctccaatc
     1081 cgcgcagcga ggcgcgtcgc gtcgcaacgc cgatcttgtt catcgcccag gccacgcccg
     1141 gcagcacgac gatggaaatc agcgccagtt gccaatcgta atagaacatc gcgcaggcgt
     1201 agaagacgag ttgcaccgcc tccatggcga tcgccgccgt gccctgcgtg atcgcatcgc
     1261 gcatcaacgt ggcgtcgtat aggaagttcg agatgaactg tcccgaatgc acggcgttca
     1321 gcgacgccag atcgcggcgg atcaggctgt cgaacatgtc gcgctgactg gcggccacga
     1381 cgcgctcgcc cagcgtgtcg atcagcgtct tctgtccgta ccacgacgcc gcccgcaaca
     1441 tcatgatgac gaccaccgcc agcgccaccg ggagcagcag gtcggcctgc cgttcgatga
     1501 agatgtgctt gatcgtgaaa tcgaggaagg cgggaatcga ggcgctggcc gccgaggtga
     1561 tcagcatgca ggtgattgcc agcgcgaaca cgctccagtg gcggctcaga tagtcgcggg
     1621 cgagccggag caggatctgc cgcggcggca tctggatcgc cgggggcggc gcgtccgtgg
     1681 gtctgatcag ggggcggggg tcgggcagag cggtcatcgg gcgccgatgt agcacggccg
     1741 gcgcgccgcc cgcgaggtct atgaccgcct ctagagcgca atccggcaaa gtgtgaagcg
     1801 gtttgccgtc agattgcgcg aaaaacaaga aatcgagagt gtgatccgat tcaattaaat
     1861 cggatcacgc tctagggcct caattttccc cttccggctc cgcctatgcc ttcacgcgcg
     1921 cgagaatcac ttcgtgcagg gcgcgtccgg cggccaccac cgagggttgg gttgtgtcgg
     1981 cccggttgta gcgggggaca gttccccggt gcgatgaaat gagccccccc gcctcccgga
     2041 cgatgagatc ggcggcggcc aggtcccagt cgtgtgtccc tgccaaggtc atggccgcgt
     2101 cggccgcccc gcacgccacc agcgcgatgc ggtaggcgat ggagttgggc gtctcgaccg
     2161 ccatcggcgg ccaagccgac ccgccccagc aagggtcgtt cagcgtcgtc ttgctggcca
     2221 gcatgtggca gcccaccagg ctttcgcgac tgctcacccg gatcggctcg ccgttcagcc
     2281 gcgcgcccgc gccgcgggcg gcggtgaagc attcctccgt aagcggatta tagaccgaag
     2341 ccgcgacggg aatctcgccc tcgaccacgg cggccgagat cgtgaaatgc ggccggcctt
     2401 tgaggaacgc caccgtgccg tcgatcggat cgacgatgaa gacgcgccgg gccgcgagcc
     2461 gcgcgggatc gtcctcggtt tcttccgaca gccaggcata gtcggggcgc gcgggccgca
     2521 gccgctcctt caggaagcga tcgacggcca gatcggcctc cgagaccggg cttccgtccg
     2581 tcttgctccg gatcttgttc tggccggtca ggaacgatct ggcgatgact ccggcttcgc
     2641 gcaccgcgtc ttcgagcagg gcggtgtcgg cggcgtcatc aagctccggc aacggtcatc
     2701 ccctcgatgc ggcaggtcgg cgcgttgacg ccgtagcgga agacgagatc gttggccggc
     2761 gtcaaggcgc ggtacatgtc cttcaggttt cccgcgatgg tgacctccga gaccgggtag
     2821 gcgatcttgc ctttttcgat ccaaaagccg gacgcaccgc ggctgtagtc gccggtgacg
     2881 gtgttgacgc ccatacccat caattcggtg acatagaagc cttcttcgat gtccgccatc
     2941 agctccgcgg gcgaaagcgc gccgggcagc atgtggaaat tggtggaggc gggatgcggc
     3001 ggtccggagg tagcccgcgc ggcatgtccg gtggtcgtca agccgagctg tttggcgctg
     3061 gcgcagtcga gcagccaggt tttcagcaag ccgttttcga cgacggtcgt aggcgcattg
     3121 gcgacgcctt cgccgtcgaa cggcttggaa cgcaggccgc gcaagcggtg cgggtcgtcg
     3181 acgatggtga tggacggcgc gaaaatttcc tggccgaggc ggtccttgag gaaactgacg
     3241 ccgcgcgcga tggcggcgcc ggagatcgcg cccgtcagat gtcccaatag gcccgccgat
     3301 tcgcgcggat cgaagaccac cggaaccgcc tgcgattttg ccttccgcgg tcccaggcgg
     3361 gcgaccgtgc gttcgccggc gcgcctgccc acggtttcgg ccgcttcgag gtcgctcgca
     3421 tgccgtgcgc tgacgctttc atagtcgcgc tccattgccg tgccttcgcc ggccagcacg
     3481 gcgacgccga tggaatgatg cgtacccgcg tagcgcccat agaagccttc gctggtggcg
     3541 agcgcgatgg ccgcgcgtcc gaaactcgcc ccgccgcctt cggagttggt gatgccgggc
     3601 accgccatcg ccgcttcttc cactgtgcgg gcgcgctcga ccagcgtgtc ggctggcggc
     3661 tcggccggat cttcgatgtc gagattcggc aaggatgtcg ccagtctttc gcgcggcgct
     3721 aggcaggcga acttgtcctc gggcgccagc ctggccatcg ccacggcgcg ttcgggcagg
     3781 gcggtgaccc cctctttgga gaaatcggtc gaggagacga aggcgatgcg ctgtcccacg
     3841 aagacgcgca ggcccacatc gctggattcg gcgcgctcga cgtcttccag cttgcccagc
     3901 cgataggaca cgctggccga gacgttctcg acgaacaagg cgtcggcggc atcggc
//
LOCUS       DASWPU010000461         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_9518, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000461 DASWPU010000000
VERSION     DASWPU010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9518"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VGC27_09810"
     CDS             <1..246
                     /locus_tag="VGC27_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282905.1"
                     /translation="MSRILWHAGYHPEIENYRRAKHRAVEASDILMARAHEEGVAALV
                     AHGYFNAIIGRELRRRGFNKTGSHRARYWNAVIYELA"
     gene            complement(403..2001)
                     /gene="purH"
                     /locus_tag="VGC27_09815"
     CDS             complement(403..2001)
                     /gene="purH"
                     /locus_tag="VGC27_09815"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY0282906.1"
                     /translation="MTLRPIRRALLSVSDKTGLADFAKGLAKHGIQLISTGGTAKALR
                     EAGLKVADVSELTKFPEMMDGRVKTLHPVIHGGLLALRDKADHNEAMRAHGIEGIDLL
                     VCNLYPFEATVAKGASFEETIENIDIGGPAMARSAAKNHDWVTVVADPDDYAAVLAEL
                     DANQGATTLALRRKLAQTAYARTAAYDAAVSNWFAQVLVKDGDEAPPRRRAFAGQLRQ
                     SLRYGENPHQGAAFYVTGEKRFGVSNAEQIQGKELSYNNINDTDAAYELVAEFDPKQS
                     AACAIVKHANPCGVALSGTLADAYTKALACDPASAFGGIIAFNRTLDGPTAEAISKIF
                     TEVIIVPDADADARAILAKKKNLRLLIAGGLPDAKAAGLSFKSVSGGFLVQTRDNGRI
                     ARADLKIVTKRPPSESEIADMLFAFIVGKHVKSNTIVYAKDLATAGIGAGQMSRVDSA
                     RIAAEKARQAAKAAGWTEPRTIGSAAASDAFFPFPDGLEEIAAAGATAVIQPGGSIRD
                     AEVIAAADQAGLTMAFTGMRHFRH"
BASE COUNT          338 a          649 c          699 g          377 t
ORIGIN      
        1 atgtcccgca tcctgtggca tgccggctat catcccgaga tcgagaatta ccgccgcgcc
       61 aagcatcgcg cggtggaggc ctccgacatc ctgatggcgc gcgcccacga agaaggggtc
      121 gccgccctcg tcgcccacgg ctatttcaac gccatcatcg ggcgcgagtt gcgccggcgc
      181 ggctttaaca agaccggctc gcaccgggcg cgctactgga acgcggtgat ctacgagctg
      241 gcttagggct atcaggcttt tgcggaattc aatccctcac ccttcgacaa gctcagggtg
      301 agggtacagc caagccctca tgatgagctt gtcgaagcat gagcctgtcg aagcatgggg
      361 ggcgcatgaa cacatcagat catcgacggc ggtaaaaccg cttcagtgcc ggaagtgccg
      421 catgccggtg aaggccatgg tcaggccggc ctggtccgcc gccgcgatca cctcggcgtc
      481 gcggatcgag ccgccgggct ggatcaccgc cgtggcgccg gcggcggcga tttcctccaa
      541 gccgtccggg aaggggaaga aggcgtccga ggcggcggcc gagcctatcg tccgcggctc
      601 cgtccagccg gcagccttgg ccgcctgacg cgccttctcg gccgcgatgc gcgccgaatc
      661 cacccggctc atctggcccg cgccgatccc ggcggtggca aggtctttgg cgtagacgat
      721 ggtattcgac ttgacgtgct tgccgacgat gaaggcgaac agcatgtcgg cgatttcgct
      781 ttcgctcggc ggacgcttgg tgacgatctt gaggtccgcg cgcgcgatgc ggccattgtc
      841 gcgggtctgc accagaaaac cgccggagac ggatttgaag ctgaggcccg ccgccttggc
      901 gtcgggcagc ccgcccgcga tcagcaggcg caggttcttt ttcttcgcca ggatggcgcg
      961 cgcgtcggcg tcggcatcgg gaacgatgat cacttcggtg aagattttgg agatcgcttc
     1021 cgccgtaggc ccgtcgagcg tgcggttgaa agcgatgatg ccgccaaacg cgctcgccgg
     1081 atcgcaggcc agcgccttgg tgtaggcgtc cgccaacgtg cccgacagcg ccacgccgca
     1141 gggattggcg tgcttgacga tggcgcaagc ggcgctttgc ttcggatcga actcagcgac
     1201 gagttcataa gcggcgtcgg tatcgttgat attgttgtag gaaagttcct tgccctggat
     1261 ttgttcggcg tttgaaaccc cgaagcgctt ctcgccggtg acgtaaaacg cggcgccctg
     1321 gtgtggattc tcaccatagc gcagcgactg gcgcagttgc cccgcgaaag cgcggcggcg
     1381 cggcggggcc tcgtcgccgt ccttgaccag cacctgcgca aaccagttgg aaaccgcagc
     1441 gtcgtaggcg gcggtgcgcg cgtaagccgt ctgcgccagc ttgcggcgca gcgccagcgt
     1501 ggtggcgccc tggttggcgt cgagttcggc aagtaccgcg gcgtaatcgt cgggatcggc
     1561 caccaccgtc acccagtcgt gattcttggc ggcggagcgg gccatcgccg ggccgccgat
     1621 gtcgatgttc tcgatggttt cttcgaagct cgcgcctttg gcgaccgtcg cctcgaacgg
     1681 atagagattg cagaccagaa ggtcgatgcc ctcgatgccg tgcgcacgca tcgcctcgtt
     1741 gtggtccgcc ttgtcgcgca gcgccaaaag cccgccatgg atgaccgggt gcagggtctt
     1801 gacccgtccg tccatcattt cgggaaattt cgtcagctcg gacacatcgg ccactttgag
     1861 gcccgcctcg cgcagcgcct tggcggtgcc gccggtcgag atcagttgga tgccgtgctt
     1921 cgccagccct ttggcgaaat cggccaggcc ggttttgtcc gaaacggaaa gcagcgcgcg
     1981 gcggatgggg cggagcgtca tggcgactcg cgaggctgga ggttacgggg ggtcatactt
     2041 cgcgcggcgg caaaagagaa gcg
//
LOCUS       DASWPU010000462         6889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_3585, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000462 DASWPU010000000
VERSION     DASWPU010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3585"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1002
                     /locus_tag="VGC27_09820"
     CDS             118..1002
                     /locus_tag="VGC27_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282907.1"
                     /translation="MIKKMLVAGLLLGAAAPALAQSNVAPPAYAPLQDYPTCTRAELK
                     AATDAYVKAQSRGDISGLPLAANAHYLENMSTNDAKAGLWNTALPIAHAMSFHDDKRC
                     KTFTEIIVTGGGHPYVIGTRLYLHGGKIIRVDSLVTDKDDWLFNAGATLKFTKREGNW
                     LDLNKYQKTAPAEMIRGANAYLDRFSDKLTDVPWGTPCTRLEGGHYTDPNGDPQATCE
                     RGIPAGVLYIVNRDYLIDEEKGVINVFCRFVDSNGMPDSHSFRFIDGKIRNVHTISVN
                     LTATPSPQADDDGGVLTN"
     gene            complement(1246..4272)
                     /locus_tag="VGC27_09825"
     CDS             complement(1246..4272)
                     /locus_tag="VGC27_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0282908.1"
                     /translation="MLASGAYAQDTEMVETVVITGSRIPVASSFDAPTPVSIVSAKDI
                     QLSGTVNVESLLSQSPQFTSSTWGGQTGNTVQANGDSGAAWVNLRGLGEVRNLVLVNG
                     RRFTVQGTRMTTDLNTVPSSLVERTEVVTGGNSAVYGSDAISGVVNFVMKKNFQGVEG
                     NAQYKFDQYTTSPIYNFDLVVGGNFDHDKGNVVMAVNYLSRAGFTQTDHGHWTSVQYT
                     DACVTPDSFSLTNPGVRLPGGVGICAACVAAGGKNGFVVGGSGDTPNGYINQLTTYAN
                     ATGTLRALYDAAGLQGMTADGLIFTNEGAGSPATYRNRNSTVDTYNLIATNLMQIPEQ
                     RWMLNTFAHYTFDPKIEGYTEFHFSSNTVSVQLTPSNSGGNMLFNTHDPEFSAPLQNL
                     FDYLDANEPAGTNCMTVGSNTNVCTTPGDGLVSLRYGKRFVENGVRRQEANRVAYRFA
                     GGFRGELGNVDADYLKDITYDVYYTYARTQQTDRQSGSISRSKLQADVLASTNGGTPL
                     CEVFGIGSLSQACIDDITVNSSVLTIAEMQGVNASVTGTAFDLPAGPVQFVAGTEWRY
                     TMAQYVPDKYTSSGDVAGLNAALPTQGSMVAHEAYGELRVPVLRDVPFVERLTVNGAF
                     RYSSYNLSSAKNIWTWSAGADWKMDGNLTFRGQFQRAIRAPNVGELYGGMAFNGGFQV
                     DPCGIDQPAAQRTDSVRAICLSQGVKLENLWTSAVQNTGGGIRYSSGGNPDLRPETSD
                     TITLGVVFTPEAVPGLATSFDFYSIQVSDMINSLAGGFGGVITNCYSQSDPNNLYCQR
                     IHRDNGTLGPNGYVDAVNANISSLKTQGFDFNSQYSFEVGAGLLSDEGRFDVSSGWNW
                     MLEMVSLPDITAPDVKSNCAGAYGNTYCFYEPMPRIKGLTRVTWNDGPASFSLSWRYI
                     GGVALDKYLIPLRRGVAGTVPENYTRPFLPDMNYFDVSATWDVNDSIQLYGGINNLFS
                     KDPPVMGTNASFANSYPGIYDAFGRVTFIGIKAKTN"
     gene            4630..4989
                     /locus_tag="VGC27_09830"
     CDS             4630..4989
                     /locus_tag="VGC27_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282909.1"
                     /translation="MRYSASPGSDREAARRTPAGRNRGRSKPVAPESGAPKSVVLDRP
                     GSLSRHPESGVRFFSSTTTYTKFADIPASKLGAKQEIYLDPGEVKNLVRARLRCGRRR
                     RSCLACSARRLGNRAGR"
     gene            5101..6510
                     /locus_tag="VGC27_09835"
     CDS             5101..6510
                     /locus_tag="VGC27_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEY0282910.1"
                     /translation="MVQLRRISYATCFTLFIGSLAASPALAQAQVSFPDRICNVKDYG
                     AEGYRDYPETEAFQKAIDDCAAKGGGTVEVPRGWWYVGPLTLKSNINFHIAQYADVHF
                     ATDPVLYGIGPDLAPLAGKREPGQPRNLFALIKITDAQNVAITGEGILDGQGYVWWEA
                     IRKTLPAGGAANQSGHAARPMMLLAARTKNFLLEGVTIMNAPAFNVMLDHTEDVTIRK
                     ITIQDPPSSPNTDAIDPFNSHNVLIEDNVISCGDDIVAIKATGVNPAHPDAAVSNIVI
                     RNNKIFAGHGISIGSDTSGGVKHVLIENNTFEGSFHGVRIKSRRGYGGEVSDIVIRNN
                     KMKDVQNVLTIAEYFQYIPVDMRVPQKLPKGAMLIVDLVYPADTDPAQPYAKNKTPYF
                     HDITIDGLEATGAEAVGIVLGVPEKEITNFTMKNVHIESKTGLIVRNATINAENTTIT
                     SEDGKPIVLQRHGRFKIAK"
BASE COUNT         1430 a         2113 c         2038 g         1308 t
ORIGIN      
        1 tagaatggcc gggaattgcg cctatactgc ccgaacgaaa cgcgccaatg ccgacgatcc
       61 gttgacgatc gtatctacgc agagaaagaa gaagcggcca ggaacaggga gaatgtcatg
      121 atcaagaaaa tgcttgtcgc cggtctgcta cttggtgcag cagctcccgc gttggctcaa
      181 tcgaacgttg cgccgcccgc ctacgcaccg ctacaggact atccgacctg cacccgtgcc
      241 gaactgaagg ctgcgaccga cgcctatgtg aaggcccaga gccgtggcga tatttccggt
      301 ctgccgctgg ctgccaacgc ccattatctg gaaaacatgt cgacgaacga tgccaaagcg
      361 ggactttgga acaccgctct gcccatcgcg catgcaatga gcttccatga cgacaagcgc
      421 tgcaagacct tcaccgagat tatcgtcacc ggtggcgggc acccctatgt gatcggcacg
      481 cgcctttacc tgcatggcgg caagatcatc cgcgtcgaca gcctcgtcac cgacaaggac
      541 gactggctgt tcaacgccgg cgccacgctc aaattcacca agcgggaagg gaactggttg
      601 gacctgaaca aatatcagaa gacggcgccg gccgagatga tccggggcgc caatgcctat
      661 ctggatcgct tttccgacaa gctcaccgac gttccctggg gcacgccctg cacacgcctg
      721 gaaggcggcc actatacgga ccctaacggc gatccccagg cgacctgcga gagaggcatt
      781 ccggccggcg tcctctacat cgtcaatcgc gactatctga tcgacgagga aaagggcgtc
      841 atcaacgttt tctgccgctt cgtcgacagc aacggcatgc ccgactccca cagcttccgc
      901 ttcatcgacg gcaagatccg taacgtgcac acgatcagcg tgaacctgac agccacgccg
      961 tcgccccagg ccgacgatga tggcggtgta ctgacgaact aaacggagat ttcccgccgg
     1021 cgcgggtgta cccgcgggca gacaaaaccc ggcaccgcgg ggcggcgccg ggttttgtct
     1081 aaagcgccgg gatttagccg aacgccctgc cgccgcattg atgccggcga aggctcgcgc
     1141 aacaccccgg cccgcggtta aaaaaacggc cggtacaaaa cttgtaccgg ccgcatcagt
     1201 tcagggtagc gcgggagagg gtaagcttca gcaccacccc atcgattagt tagtcttggc
     1261 cttgatgccg atgaacgtta cgcgaccgaa ggcatcgtat attccggggt agctgttcgc
     1321 gaagctcgcg ttggtaccca tgaccggcgg atccttcgag aacaggttgt tgatgccgcc
     1381 gtagagctgg atgctgtcgt tgacgtccca cgtcgcagaa acatcgaagt agttcatatc
     1441 cggcaggaac ggacgcgtgt agttctcggg aacggtgccg gcaactccgc gacgcagcgg
     1501 aatgaggtac ttatccagcg ccacgccacc gatataacgc cagctcaggc tgaacgaagc
     1561 cgggccgtcg ttccaggtaa cacgcgtcaa gcccttgatc cgaggcatag gctcatagaa
     1621 gcagtaggtg ttgccatatg cgccggcgca gttgctcttg acgtcggggg ccgttatgtc
     1681 gggcagcgag accatctcca acatccagtt ccagcccgaa ctgacatcga agcggccctc
     1741 gtcgctcagc aggcccgcgc cgacttcaaa gctgtactgg ctgttgaagt cgaagccctg
     1801 ggtcttcaag ctggagatgt tggcgttcac cgcatcgaca taaccattcg ggcccagcgt
     1861 accgttgtcg cggtgaatcc gctggcagta gaggttgttc ggatcgctct ggctgtagca
     1921 gttggtaatg acgccaccga agccaccggc cagggagttg atcatgtcac tcacttggat
     1981 gctgtagaaa tcgaagctcg tcgcaagtcc gggaaccgct tcgggcgtga acaccacacc
     2041 aagcgtgatc gtatccgagg tttcaggcct caagtcgggg ttgccgccgc tcgagtagcg
     2101 gatgccgccg cccgtatttt ggaccgcact ggtccacagg ttttccagct tcacgccctg
     2161 cgacagacag atagccctca cggaatccgt ccgttgagcg gccggctgat cgatgccgca
     2221 aggatcgacc tggaaaccgc cgttgaacgc catgccgccg tacagctcgc cgacgttcgg
     2281 cgcacggatg gcgcgctgga actggccgcg gaaggtcaag ttgccgtcca tcttccagtc
     2341 ggcgccggca gaccatgtcc agatgttttt cgccgaagac agattgtagc tcgaataccg
     2401 gaaggcaccg ttgaccgtga gcctctcgac aaacggcaca tccctgagga ccggaacgcg
     2461 caattcgccg taggcttcat gcgccaccat cgagccctgc gtgggcaagg cggcattcaa
     2521 gcccgcgacg tcgcccgaac tcgtgtactt gtcgggcaca tactgggcca tggtgtaccg
     2581 ccattcggtg ccggccacga actggaccgg gccggccggc aggtcgaagg ccgtgccggt
     2641 gacgctggcg ttgacgccct gcatctcggc gatcgtcagc acggaggagt tcaccgtgat
     2701 gtcgtcgatg caggcttgcg acagcgaacc gatgccgaag acttcgcaca acggcgtacc
     2761 gccgttggtc gaggcgagca cgtcggcctg cagcttgctc ctggagatcg aaccgctctg
     2821 gcggtcggtc tgctgagtgc gggcgtaggt gtaatagaca tcataggtga tgtctttcag
     2881 gtagtcggca tcgacgttgc cgagttcgcc gcggaagccg ccggcaaacc gatatgccac
     2941 gcggttggct tcctggcggc gcacgccgtt ttcgacgaag cgcttgccgt aacggagcga
     3001 caccaagccg tcgcccgggg tggtgcaaac gttggtgttc gagccgaccg tcatgcagtt
     3061 ggtgcccgcc ggctcgttgg catccaggta gtcgaagaga ttctgcaacg gcgcggagaa
     3121 ctccggatca tgcgtattga acagcatgtt gccgccggag ttggatggcg tcagctggac
     3181 ggaaaccgtg ttgctcgaga agtggaactc cgtataaccc tcgatcttcg gatcaaaggt
     3241 gtagtgcgcg aatgtgttca gcatccagcg ctgttccggt atctgcatca ggttggtggc
     3301 gatcaggttg tacgtatcga cggtagaatt cctgttgcgg tacgtggccg gagaaccggc
     3361 cccttcgttc gtgaagatga ggccgtccgc cgtcatgccc tggagtccgg cggcgtcgta
     3421 aagtgccctc agagtgccgg tggcgttggc gtaggtcgtg agctggttga tatagccgtt
     3481 cggcgtatcg ccgctgccgc ccaccacgaa gccgttcttg ccgccagcgg cgacgcaggc
     3541 cgcgcagatg ccgacaccac cgggcaagcg cacgcctggg ttggtcaggc tgaaggagtc
     3601 cggcgtcacg caggcgtcgg tgtactggac gctggtccaa tggccgtggt cggtctgggt
     3661 gaagcctgca cggctcagat agttcacggc catgacgacg ttgcccttgt catggtcgaa
     3721 attgccgccg acgacgaggt cgaagttgta gatcggcgat gtcgtgtatt ggtcgaactt
     3781 gtactgcgca ttgccttcca caccctggaa gttcttcttc atgacgaagt tgacgacgcc
     3841 tgagatggca tccgagccgt acaccgccga gttgccgccc gtcacgactt ccgtccgctc
     3901 caccagcgaa gacggaaccg tattcaggtc ggtcgtcatc ctggtgcctt ggacggtgaa
     3961 acgccgcccg ttgaccagaa ccaagttgcg cacttcgccg aggccgcgca ggttgaccca
     4021 cgccgcaccg ctgtcgccgt tcgcctgaac cgtgttgccc gtctggccgc cccaagtgga
     4081 ggacgtgaac tgcggcgact ggctcaacag gctttcgacg ttgaccgtgc ccgacagttg
     4141 gatgtccttg gcgctcacga tcgaaaccgg cgtcggcgcg tcgaacgaac tcgccacggg
     4201 aatacgcgaa ccggtgatca ccaccgtctc caccatctcc gtgtcctgcg cgtaggcccc
     4261 tgaagcgagc atcgcgctcg cagcaacaag acctgcgttg cacaaaaggc gtgtacggcg
     4321 tctctgtaat tttgtgttgt gcattttcat aaacctccct cagcacgctt cccctttggg
     4381 ctcatttgtt gtcggagctg tcatgacaca cagctcagaa ctgcgcctcc acgatcacat
     4441 gcgtgcgttc ttgtgatcga ttaggcttga taataatcac aaatgaccac ataatggtag
     4501 atgcatacgc gatattgatc gtttttgatc gaaatagtga tcaatttggt acaggcctga
     4561 gagatgcgat taacaccgct tttggtcgtt tcttttggta tttcggacag gcagcgaagc
     4621 ctgtgcccca tgcgttattc tgcctcaccg ggttctgacc gcgaggccgc tcggcgcacc
     4681 cctgctggtc gcaatcgcgg cagatccaaa ccggttgcac cagaatccgg cgcgccaaaa
     4741 tccgtcgtcc tcgaccggcc gggatcgctg tcccgccatc ccgagtccgg cgtgcgcttt
     4801 ttttccagta cgaccaccta cacgaaattc gcggacatcc cggcctccaa gctgggggcc
     4861 aagcaggaaa tctatctcga tccgggcgag gtgaagaact tggtccgggc gcggctgcgt
     4921 tgcggacggc gcagacggtc gtgcttggca tgcagcgcga ggcggctcgg aaatcgagcg
     4981 ggaaggtagt caagcggcgt ttcccgccgc ccacgcaaat aagaccgcag cgaacgacaa
     5041 tctgtcttta tccatgccgg cggcaaatgc cagccatcaa aacgggagga agaaacgcaa
     5101 atggttcaac ttcgtagaat aagttacgca acgtgcttca cactcttcat cggttcactc
     5161 gcggccagcc ccgcacttgc ccaggcgcag gtttccttcc ccgaccggat ctgcaacgtc
     5221 aaggattacg gcgccgaagg ctaccgggac tatcccgaga cggaagcctt ccaaaaggcg
     5281 attgacgatt gcgccgccaa aggcggcggc acggtggagg ttccgcgcgg ctggtggtac
     5341 gtcgggccgc tgaccctgaa gagcaacatc aacttccaca tcgcccaata tgccgatgtg
     5401 cacttcgcga cggatcccgt gctttatggc atcgggcctg accttgcccc gctggcgggc
     5461 aaacgggaac cgggccagcc gagaaacctc ttcgccctca tcaagatcac ggatgcccag
     5521 aatgtcgcca ttaccggcga gggcatactc gacggacagg gctatgtctg gtgggaagcg
     5581 atccgcaaaa ccttgccggc tggcggcgca gccaaccaaa gcggtcacgc tgcccggccc
     5641 atgatgctgc tcgccgcgcg caccaagaac ttcctcctgg aaggcgtgac catcatgaac
     5701 gcgccggcct tcaacgtcat gctggatcac acggaagacg tcaccatccg caagatcacg
     5761 attcaggacc cgccatcctc gcccaacacc gacgccatcg atcccttcaa cagccacaac
     5821 gtgctgatcg aagacaacgt catcagttgc ggcgacgata tcgtggccat caaggcgacc
     5881 ggggttaatc ccgcacaccc cgatgcggcg gtttccaata tcgtgatccg caacaacaag
     5941 atttttgccg gtcacggtat ttccatcggc agcgacacct cgggcggcgt caagcatgtg
     6001 ctgatcgaga acaacacctt cgagggctcg ttccacggag tccggatcaa gtcgcggcgc
     6061 ggttacggcg gcgaagtgtc ggacatcgtc atccgcaaca acaagatgaa agacgttcag
     6121 aacgtgctga ccatcgccga gtatttccag tacataccgg ttgacatgcg ggtgccgcag
     6181 aagcttccga agggcgccat gctcatcgtg gacctggtgt atccggccga caccgacccg
     6241 gcccagcctt atgccaagaa caagacgccg tacttccatg acataacgat cgacggcctg
     6301 gaagccaccg gtgcggaagc ggtaggcatc gtgctcggcg ttccggaaaa ggagatcacc
     6361 aacttcacga tgaagaacgt gcacatcgaa agcaagaccg gtctgatcgt gcgcaatgca
     6421 acgatcaacg ccgaaaacac cacgatcacg tcggaagacg gcaagcccat cgtgctgcag
     6481 cgtcacggcc gattcaaaat cgcgaagtga tccgcgaata gatcagcctc aacaaaactg
     6541 ccctgccggg ccccccggca gggctttttg ttagccccct gccttcttac tgcgcggcgg
     6601 aaaaatggga ggatggttat gcggaggcgg atagctccgt ccacccggtt gagcctctcc
     6661 ctcatgcgag gagagtgaaa ggcggttcgg gtttccgtga cgggttgatg gccagcgtgt
     6721 cctgagatcg taatcacctg acagtccggc ccgtcggcag gcgcgcgctc cgcaaggtaa
     6781 gcggcgccat catcatcaga cggcttgatt ttcgagcaac caacggatga tcgtaccgcc
     6841 caggcccgct gcacccccag gatgattctt ggagccttgg ctattcgtc
//
LOCUS       DASWPU010000463         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_35000,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000463 DASWPU010000000
VERSION     DASWPU010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35000"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGC27_09840"
     CDS             complement(<1..188)
                     /locus_tag="VGC27_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282911.1"
                     /translation="MDKEVREGSRISRLPRSIQPLAVIVREGLVRPLLARAHRETLAD
                     FHHVLDGIATKHIDRLRE"
     gene            complement(504..>2757)
                     /gene="acnA"
                     /locus_tag="VGC27_09845"
     CDS             complement(504..>2757)
                     /gene="acnA"
                     /locus_tag="VGC27_09845"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF009520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY0282912.1"
                     /translation="EYERNMERYAFLRWGQQAFANFRVVPPGTGICHQVNLEYLAQTV
                     WTRKDKNKQGKSVAYAFPDTLVGTDSHTTMVNGLSVLGWGVGGIEAEAAMLGQPISML
                     IPEVVGFKLTGKLREGVTATDLVLTVTQMLRRHGVVGKFVEFFGPGLDDMALEDRATI
                     ANMAPEYGATCGFFPIDAETLRYLKDTARKPARLALVEKYAKAQGLYRTARNEDPVFT
                     STLGLNLASVEPSLAGPARPQDRVPLSDAANAFADALVNTYNKGGDAGRRVPVEGTDY
                     TIGHGDVVIAAITSCTNTSNPNVMIGAGLIAKKAVEKGLKVRPWVKTSLAPGSQVVTD
                     YLAKAGLNTYLDKLGFQLVGYGCTTCIGNSGPLPENVAGAITGADLVAAAVLSGNRNF
                     EGRVHAQVRANYLASPMLVVAYALAGTINLDLTKEPIGTGKKGEPVYLKDVWPSPKEV
                     ADTIRKSVKASAFRSRYGDVFEGDANWRKVKVKKGQTYQWDEASTYVKNPPYFDGISI
                     APAPLADIKGARILALFGDSITTDHISPAGSIKVTSPAGVYLQEHGIALKDFNSYGSR
                     RGNHEVMERGTFANIRIRNEMLGGAEGGNTIHYPSGDAMAIFDAAARYKAESVPAVVI
                     GGKEYGAGSSRDWAAKGPKLLGVRAVITESFERIHRSNLVGMGVAPLVFETGRTRKDY
                     ALTGEEIIDIPGLSGTLTPRLKIEATIRYPDGRTAPLPLVLRIDTADEVAYFTNGGIL
                     PYVLRNLVAG"
BASE COUNT          467 a          877 c          889 g          524 t
ORIGIN      
        1 ttttcccgta aacgatcaat gtgcttggtg gctatgccgt cgagcacatg gtggaaatcg
       61 gccagagttt cacggtgggc gcgggccagt aagggcctaa ccaagccttc gcgcacgatt
      121 acagcaagcg gttgaatcga cctaggtaat ctcgatatcc tcgaaccttc tcgaacttcc
      181 ttgtccatct tcgcggcgac tccgctctct ggcagccgaa gggtcgatga gcctcagtat
      241 ggagagccgc acgcgccttt aggaatacgc cctggacaca ggcacacgct tcctcggcct
      301 tctctgggtc caggaatgct tgcagtggca caacactgcc atacgaggaa tcatcaagcc
      361 ccaagccggc caatgggccg gcttgagaag acgttagctc cgagaactcc ctgagtcgag
      421 tttggggtct tgctcggccg ggctgcctcg actgggcttg ggtttaacct tggcggccgc
      481 gcggggtgga ctgacgccgg cgtttacccc gccaccaaat tcctcagcac gtaaggcaga
      541 atcccgccat tggtgaaata ggccacctcg tccgccgtat cgatgcgcag gacgagcggc
      601 aagggcgccg tgcggccgtc gggatagcgg atcgtcgcct cgatcttcag gcgcggcgtc
      661 agcgttccgg aaaggccggg aatgtcgatg atctcctcgc cggtcagcgc atagtccttg
      721 cgcgtcctgc ccgtttcgaa aaccagcggc gccaccccca tgccgaccag attggagcgg
      781 tggatacgct cgaagctctc ggtaatcacc gcgcgcacgc cgagcagctt cggccccttg
      841 gcggcccaat cgcgcgagga gccggcgccg tattccttgc cgccgatgac gacggcggga
      901 acgctctcgg ccttgtagcg cgcggccgcg tcgaagatcg ccatcgcatc gcccgaggga
      961 tagtggatgg tgttgccgcc ttcggcgccg cccagcatct cgttcctgat gcggatgttg
     1021 gcgaaggtgc cgcgctccat cacctcgtga ttgccgcgcc gcgagccgta ggagttgaaa
     1081 tccttgagcg cgataccgtg ctcctgcaga tagacgcccg cgggcgaggt taccttgatc
     1141 gatccggccg gcgagatgtg gtcggtggtg atggaatcgc cgaacagcgc caggatgcgc
     1201 gcgcccttga tgtcggcgag cggcgcgggc gcgatgctga tgccgtcgaa ataaggcggg
     1261 ttcttcacat aggtggaggc ttcgtcccat tgataggttt ggcccttctt gaccttcacc
     1321 tttcgccaat tggcgtcgcc ctcgaagacg tcgccgtagc gcgaccggaa agcggacgcc
     1381 ttcacgctct tgcggatggt gtcggcgacc tccttgggcg acggccaaac atctttcagg
     1441 taaaccggct cgcccttctt gccggtgccg ataggctcct tggtgagatc gagattgatc
     1501 gtgccggcca gcgcataggc caccaccagc atcggcgagg cgagatagtt ggcccgcacc
     1561 tgcgcatgca cgcgcccttc gaaattgcgg ttgcccgaga gcaccgcggc ggcgacgagg
     1621 tccgcgccgg tgatcgcgcc cgcgacattc tccggcagcg gcccggaatt gccgatgcag
     1681 gtggtgcagc cgtaaccgac gagctggaag ccgagcttgt cgagataggt gttgaggccc
     1741 gccttggcga gatagtccgt caccacctgg ctgccgggcg ccagcgaggt cttcacccag
     1801 gggcgcacct tcaagccctt ctccaccgcc ttcttggcga tcaggccggc tccgatcatg
     1861 acgttgggat tcgaggtgtt ggtgcaagag gtgatcgccg cgatcaccac gtcgccgtgg
     1921 ccgatggtat agtccgtgcc ttccaccggc acgcggcggc ccgcatcgcc gcccttattg
     1981 taagtgttga cgagcgcatc ggcgaaggcg tttgcggcgt cggacagcgg cacgcggtcc
     2041 tgcggccgcg ccggaccggc gagggaaggc tccacgctcg ccaaattaag ccccagcgtc
     2101 gaggtgaaca ccggatcttc gttcctggcg gtgcggtaga ggccctgcgc cttggcgtat
     2161 ttctcgacca gcgccaggcg cgccggcttg cgcgcggtgt ccttcagata gcgcagcgtt
     2221 tccgcgtcga tggggaagaa gccgcaggtg gcgccgtatt cgggcgccat gttggcgatg
     2281 gtggcgcggt cttccagcgc catgtcgtcg agacccggcc cgaagaattc cacgaacttg
     2341 ccgaccacgc cgtgccggcg cagcatctgc gtgaccgtca gcaccagatc ggtggcggta
     2401 acgccttcgc gcaatttgcc cgtcagcttg aaaccgacca cttcgggaat gagcatggag
     2461 atcggctggc cgagcatggc cgcctcggcc tcgatgccgc ccacgcccca gcccagcacc
     2521 gaaagcccgt tgaccatcgt ggtgtggctg tcggtgccga ccagcgtgtc ggggaaggca
     2581 taagcgacgg acttgccctg cttgttcttg tctttgcgcg tccacaccgt ctgggcgaga
     2641 tattcgagat tgacctgatg gcagatgccg gtccccggcg gcaccacgcg gaaattggcg
     2701 aaggcctgct ggccccagcg caggaacgca tagcgttcca tgttgcgttc gtattcc
//
LOCUS       DASWPU010000464         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_101628,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000464 DASWPU010000000
VERSION     DASWPU010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101628"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VGC27_09850"
     CDS             complement(<1..594)
                     /locus_tag="VGC27_09850"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0282913.1"
                     /translation="MTSPAVFIRDNTALTAPPLVPELRLHLATEIVPLWRKTEEELEE
                     QGVPPPYWAFAWAGGQALARYVLDHPDAVRGKSVLDFGSGSGLVAIAAVKAGASQVLA
                     ADIDAFAATAIRLNADANGASLAITQEDVIGRAGDWATILAGDMCYERPLAERLLVWL
                     KQRVQAGATVLLGDPGRSYFPPSGIEKLATYRVETTRE"
     gene            786..1568
                     /locus_tag="VGC27_09855"
     CDS             786..1568
                     /locus_tag="VGC27_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282914.1"
                     /translation="MSKHFAGIRILLIFVGINLVMFLMLVTSMTSAHPWLAHLLPGHF
                     EAGVNSSGYWVFFALVLLVDAVTVLIAGLALLLPAMLDSSAVEERRLARHLVDRGGVS
                     EEAKESIFVALREEVVAAHRQIVLGRTILFAGLVFLVFAFFAMSFSFARAQPDGMMYA
                     DAAGPVRNDTVRAWDIERFTVDQIAGAVLLNAPDIYDWHFGTLRNNSARPVYTNFVFA
                     FRAVMGFVLLLIVISLFRRGERPSRDKKTIESVEAAAEEKKG"
     gene            1694..>1979
                     /locus_tag="VGC27_09860"
     CDS             1694..>1979
                     /locus_tag="VGC27_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282915.1"
                     /translation="MTTPAQSMETEPRCIYCPNPKQTKEHMPHKDMFCGKLRPKGMEF
                     DACKNCNSGTSVSDAVAVIIACWSPDFGKGSWQLNEKIRKLRSTIAQKAPD"
BASE COUNT          374 a          591 c          616 g          398 t
ORIGIN      
        1 ctcgcgcgtc gtttcgacgc gataggtggc gagcttctcg attcccgacg gcgggaaata
       61 gctgcgcccc ggatcgccca acagcacggt cgccccggcc tgcacgcgct gtttcagcca
      121 gaccagcagc cgttccgcca gcggccgttc gtagcacatg tcgccggcca gaattgtcgc
      181 ccaatcgccc gcccgcccga tcacatcttc ctgagtgatg gcaaggctcg cgccgttggc
      241 gtccgcattc agccggatgg cagtcgccgc gaacgcgtcg atgtcggcgg ccagcacttg
      301 cgacgcaccc gctttcaccg cagcgatcgc caccagcccc gaccccgagc cgaaatcgag
      361 caccgatttt ccgcgcacgg cgtcgggatg atccagcaca tagcgcgcca gggcttggcc
      421 gcccgcccag gcgaaagccc aatagggcgg cggtacgcct tgttcctcaa gctcttcttc
      481 ggtttttcgc cacagcggca cgatttcggt cgccagatgc aggcgcaatt cgggaaccaa
      541 gggcggggcg gtcagggcgg tgttgtcgcg gatgaacacg gcgggacttg tcatgtgcgg
      601 gaggcacctt tcgtgtatga tgtctagcac gtgatccagt ctaacaggat cgctccggtc
      661 gcccttcgac aggctcaggg tgaggggagt gcctaatcct tcatgctgag cttgtcgaag
      721 catgtgtgtg gcgcgatccc atgtaggtgg atcacgctct aacacaacta gcgttggggg
      781 gcggtatgtc gaagcatttt gcgggcatca ggattttgct gattttcgtc ggcatcaacc
      841 tggtgatgtt cctcatgctg gtcaccagca tgaccagcgc tcacccgtgg ctggcgcacc
      901 tgttgccggg acatttcgaa gccggggtga attcgtccgg ctattgggtg tttttcgcgc
      961 tggtgctgct ggtcgacgcg gtcaccgtcc tcatcgccgg cctggcgctt ctgcttcccg
     1021 cgatgctgga cagttccgcg gtcgaggagc gccggctggc gcgccatctg gtggaccgcg
     1081 gcggcgtttc ggaggaggcg aaggaatcca tcttcgtggc gctgcgcgag gaggtggttg
     1141 cggcccatcg ccagatcgtc ctggggcgga cgatactgtt tgccggcctc gtcttcctgg
     1201 tgttcgcgtt cttcgccatg tcgttcagtt tcgcccgcgc gcagcccgac ggcatgatgt
     1261 acgccgacgc ggcgggcccg gtgcggaatg acacggtgag ggcctgggat atcgaacgct
     1321 tcaccgtgga tcagatcgcg ggcgccgtgc tgctcaatgc gccggatatc tacgactggc
     1381 atttcggcac gctgaggaac aacagcgcca ggccggtcta caccaatttc gtcttcgcct
     1441 tccgcgcggt catgggcttc gttctgctct tgatcgtgat cagcctgttt cgccgcggcg
     1501 aaaggccgag ccgggacaag aagaccatcg aaagcgtgga agccgcggcg gaagaaaaga
     1561 agggctgacc gaaccgcggc gaagggcgcg acggctgaaa ttgtctccgg ccagtttttt
     1621 tcagcgcagg aaacccagca tggccaacga cataatgcga cgggaaatca ccctctacta
     1681 acggaagttc catgtgacga cacccgcgca gagcatggaa acagagccac gctgcattta
     1741 ctgcccaaac cctaagcaga ccaaagagca tatgcctcat aaggatatgt tttgtggcaa
     1801 gttacggccc aaagggatgg aatttgacgc atgcaaaaac tgcaacagcg gtactagcgt
     1861 ctcggatgca gtagccgtca tcattgcttg ttggtcgcca gattttggca aaggtagctg
     1921 gcaactgaac gagaagataa gaaagcttcg ttcaactatc gctcaaaaag cgcctgatg
//
LOCUS       DASWPU010000465         2788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_35002,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000465 DASWPU010000000
VERSION     DASWPU010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35002"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(376..555)
                     /locus_tag="VGC27_09865"
                     /pseudo
     CDS             complement(376..555)
                     /locus_tag="VGC27_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611367.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(733..1791)
                     /locus_tag="VGC27_09870"
     CDS             complement(733..1791)
                     /locus_tag="VGC27_09870"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY0282916.1"
                     /translation="MSRRKSMAVLAGAAAIAGTCRASDAGGRSTRPAAAGILKPRRLP
                     SGGTIAVPAPASPAFLRSEILRGQAVWMQAGYRVRVLDQVYETDGYLAGAPRDRARAL
                     QECFRDPGIDAIHVAGGGYGATQLIPSLDFDIIASHPKPFIGRSDITALHLALARYCG
                     LVTLYGPGLVSVASPNGSSFSETGLLRAATDPSPLGLLPRHPSDPFLFTITGGTASGP
                     LRGGCLWPLCKTVGTDWAPQLDGAILFLEETDEPPWSIDAHLTHLIQAGLLDRVAGIV
                     IGRMVNCNWSASRPEYPSNLSLEDVLLRRLGPLGVPCLYGIPVGHEHDTLTIPLGVRA
                     TLDATAQTLTFEESAFAG"
     gene            complement(1788..2102)
                     /locus_tag="VGC27_09875"
     CDS             complement(1788..2102)
                     /locus_tag="VGC27_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282917.1"
                     /translation="MPVLREWRALVRRELSREYLEYVKATGIPAYRGTPGNLWALAAL
                     RDLDAERSEIVTLSLWSSLEAIGAFAGDDLDRARYFPEDDRYLLTRPERVAHYSCDGT
                     PC"
     gene            2172..2768
                     /locus_tag="VGC27_09880"
     CDS             2172..2768
                     /locus_tag="VGC27_09880"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEY0282918.1"
                     /translation="MEQAARAIEDIRLNGGRPCVDFVNTIHDRNAAPQEDYLTTPERL
                     LAWAARAGLVHAGEKLAAPTRADQRARLMSETRRLREAVHALLSARIEGTAAPQGALR
                     TLDRWVHAAWKDFVLTGLAGGRAAVKNASRTAKLVLMRASLCALDLLSGEQAANLKCC
                     PECGWLFLDTSKNGSRRWCSMETCGAVVKMRNYRKRSG"
BASE COUNT          529 a          892 c          885 g          482 t
ORIGIN      
        1 gatatatggc tccggttcgt attttctcgg gttggccagc tttgggctgt agaattgacg
       61 aggtgcacat tctcgtttca ttctttcggc cgcaaatcgc tcgatctcgc gctgacgtcc
      121 tcgcacttgg ccgctgcgga agataattat aagtggctct tataagttca tgcgcattct
      181 gccgcctaat catgtaccgc ggcatctctt ataacgagcc gatgctcagc tgccccacga
      241 catacgcggc acggacgcag atttttacca agtgccggaa ttcggttcgg ccagtgagcc
      301 ggacagcatc gaccgaggac gagcgtccgg gccagcaacg caggcgcggc catcgccata
      361 cggctccttt tctggcttcg gctgcttcgg ccctgtcggc agatcggcaa cgaagctgcg
      421 cttcttccac ttggcgacgg tcttctggtt gatgccgtat cgcttggaaa gcgccctcag
      481 gctctcttga ctatgctgta tcgctcgacg gaccgcctct gtcgtcgtgg cgctgccatg
      541 aagaacttgt cccatagtgc ctccttccat cccccggaca agattgcacc atcaaatgcc
      601 gggactaaac acctagagcg caatccggca aagtggtcgg tagcagcagc gtgccgacgc
      661 ggtttgccgt cagattgcgc gaaaatcaag gaatctagag cttgatccga tttaattgaa
      721 tcggatcaag ctctagcccg cgaaggcgga ttcttcgaac gtaagtgtct gggccgtggc
      781 gtcgagcgtt gcccgcacgc cgagaggaat cgtcagcgtg tcgtgttcgt gaccgaccgg
      841 aatgccatag aggcacggca cgcccagggg acccaggcgg cgcagcagga catcctcgag
      901 cgacaggttc gacggatatt ccggccgcga cgccgaccaa ttgcagttga ccatccggcc
      961 gatgacgatg ccggcgacgc ggtccagcaa tccagcctgg atcaggtgcg tcagatgcgc
     1021 gtcgatgctc cacggcggct cgtcggtttc ctccaggaag aggatcgccc cgtcgagctg
     1081 cggcgcccaa tcggtgccaa cggtcttgca cagaggccag agacatccgc ccctcaaagg
     1141 gccgctggcc gtgccgcccg tgatcgtgaa caggaatggg tcggaaggat gccgcggcaa
     1201 gaggccgagc ggcgaaggat cggtcgcggc gcgcagaagc cccgtttccg agaacgacga
     1261 accgttggga gaggcgacac tcaccagacc cgggccgtac agtgtgacga gaccgcagta
     1321 gcgcgccaat gccaagtgca gcgccgtgat gtcggagcgt ccgatgaacg gcttgggatg
     1381 gctggcgatg atgtcgaagt cgaggctcgg gataagctgc gtggcgccgt agcctccgcc
     1441 ggcgacatga atggcgtcta tgccgggatc gcgaaagcac tcctgcagcg cacgcgcgcg
     1501 atcccttggc gcgcccgcga gatagccgtc cgtctcgtag acttggtcca gcacgcggac
     1561 acggtagccg gcctgcatcc acaccgcctg accgcgcaaa atctcgctac gcaggaacgc
     1621 aggcgaggca ggcgccggaa ccgcaatcgt ccctccgctc ggaagccggc gcggcttgag
     1681 aatgcccgca gccgccgggc gggtgctacg gccaccggca tcggacgcgc ggcaagtgcc
     1741 ggcgatcgcg gcggcgccgg ccagcaccgc catcgacttg cgccggctca acacggcgtc
     1801 ccgtcgcaac tgtagtgcgc cacccgctcc ggccgcgtaa gcaggtatcg gtcgtcttcg
     1861 ggaaaatagc gggcgcggtc gaggtcgtcg cccgcaaacg ccccgatcgc ctccagggag
     1921 ctccacaggc tcagcgtgac gatctcggac cgttccgcat cgaggtcgcg aagcgccgcg
     1981 agcgcccaca ggttgccggg cgtcccgcga taggccggaa tacccgtcgc cttcacgtat
     2041 tccagatatt cccgactcaa ttcccgccgc acgagggcgc gccattccct gagaacaggc
     2101 ataattgggc tttccttccg gatgcgttac cagtaaacat aattttgatg gtaacgtcaa
     2161 ggcagggcga aatggagcag gccgcacgcg cgatcgagga catcaggctg aacggcggaa
     2221 ggccgtgcgt ggatttcgtc aacacgatcc atgaccgcaa tgcggccccg caagaagact
     2281 atctcaccac gcccgagcgg ctgctcgcct gggcggcgcg ggccgggctc gtgcatgccg
     2341 gcgagaaact cgccgcgccg actcgggcgg atcagcgcgc acgtctgatg tccgaaaccc
     2401 gccggctgcg cgaagccgtc catgccttgc tgtcggccag gatcgaaggt acggcggcgc
     2461 cgcaaggcgc gctgcgcaca ctcgaccgct gggtccatgc cgcgtggaag gactttgtcc
     2521 tcaccggcct cgcaggcggg cgcgccgcgg tgaagaatgc atcgaggacg gccaaactcg
     2581 tgctcatgcg cgcgtccctg tgtgcactcg acctgttgag cggcgaacag gcggccaatc
     2641 tcaaatgctg tccggaatgc ggctggcttt tcctcgatac cagcaagaac ggctcgcgcc
     2701 gctggtgctc catggagacc tgcggagccg tcgtgaagat gcggaactac cgcaagcgaa
     2761 gcggctgagc gcgcctgcga tcaatccg
//
LOCUS       DASWPU010000466         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_101631,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000466 DASWPU010000000
VERSION     DASWPU010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101631"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..993)
                     /locus_tag="VGC27_09885"
     CDS             complement(46..993)
                     /locus_tag="VGC27_09885"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY0282919.1"
                     /translation="MRIFRHIADVPEAYKGAVIAVGNFDGVHLGHQALIGEARRLAEE
                     RGAPLAVMVFEPHPREFLRPGGEPFRLTPFRLKSHLIAALGVDAMFALAFDAALAHKS
                     APDFVLDILVNGLGAGCVVVGRDFRFGEGRSGDVAVLAYMGKMEGFGVVAVDPVSAGG
                     AGKISSTGIRKMLKLGKPEEAMRLLGHPFTVEGRVEHGDRRGRTLGFPTANLRLDGHM
                     RPAYGIYAARIAVLEEDKVVSRHDGVANLGIRPMYQSAEPLLEVFLFDFSGDLYGKHL
                     SVELIAYLRPEAKFAGVEELKVQMAKDCQNAKAAFAKTL"
     gene            complement(1001..1423)
                     /locus_tag="VGC27_09890"
     CDS             complement(1001..1423)
                     /locus_tag="VGC27_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0282920.1"
                     /translation="MTYTIEELKPGMSESFAKTVSERDVALFGEVSGDLNPAHFDEDF
                     AKTTVFKGRIVHGALVLSYLSTVLGTKMPGPGAVFLSQTTRFKAPVRIGDTVTAVCTV
                     RKVHLASRCVIFDCLCKVKDTVVVSGEATVMVPLREAA"
     gene            complement(1462..2349)
                     /locus_tag="VGC27_09895"
     CDS             complement(1462..2349)
                     /locus_tag="VGC27_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEY0282921.1"
                     /translation="MTKWSNPDTKRLQPMTSVPTIISGLREIAGGYDALICDVWGVMH
                     DGLHPHLAAVEALRRFRAEQGLVVLLSNAPRPSAPIEQQLTQIGVPAGCYDAIVTSGA
                     AAREDIERRAAAGSLAMMHIGPERDRPLYDGLNVELVGPEKAAVALVSGLYDDEIETP
                     ENYRGVLAELKNRKLPMVCANPDIFVPRGGKLLYCAGALARAYEEIGGEVVYYGKPHL
                     PIYKVALEAAGSPKHPLVVGDGLETDMLGANRLGIDALFIADGVHGEEVGALTPGNLA
                     ALFAKKGVYARAAMGALAW"
     gene            complement(2544..3791)
                     /locus_tag="VGC27_09900"
     CDS             complement(2544..3791)
                     /locus_tag="VGC27_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0282922.1"
                     /translation="MVRILLNVIYGLASLTLGSLAGLYLGLSTGILCGLIALLAMLQA
                     DSALAQRRFRKAHRSEIVNLKRANLAMEKAVAETRAKMDEVTKAVEARASAQGKKIVS
                     ELQVLESLMREFAGRISHKAKAEPAEESFAERRVRAPAPATLEGLAEPELLETIRASL
                     EENRVDLYLQPIVSLPQRKLRYYEALSRLRAEDGAVIMPAQYIKVAAPAGLMSVVDNL
                     LLFRCVQIVRKLTSKNRGIGVFCNISGDTLRDAEFFPQFLEYMHNNRDLAGQIVFEFE
                     QSAVLKAGVEGEANLNYLSGLGFALSMDHVETLALNFEKLKNMGFKHLKVRAETLTQG
                     MGTAQAAVAAEDFKKLLERHGLNLIAERVEEEKTVLQLLDYAVDFAQGYLFGEPRAVR
                     DDVIKPVEKAETRAPVIPFRRAG"
BASE COUNT          804 a         1441 c         1376 g          805 t
ORIGIN      
        1 cgccttcggc ggatcgacat cctcctttca gggggaggaa aggagctaca acgtcttcgc
       61 aaatgccgct ttggcgtttt ggcagtcttt cgccatctgc accttcagtt cctccacccc
      121 cgcgaatttg gcctccgggc ggaggtaggc gatcagctcc accgacaggt gcttgccgta
      181 aagatcgccc gaaaaatcga acagaaagac ctccagcagc ggctcggcgc tctggtacat
      241 cgggcggatg ccgagattgg cgacgccgtc gtggcgcgaa accaccttgt cttcctccaa
      301 aacggctatg cgcgcggcat agatgccgta agccggccgc atatggccgt cgagccgcag
      361 attggcggtg ggaaaaccga gcgtgcggcc gcgccggtcg ccatgttcca cccgcccttc
      421 caccgtgaac ggatggccga gcaggcgcat cgcctcttcc ggtttgccca gcttgagcat
      481 cttgcgaatg ccggtcgaag aaatcttgcc ggcgccgccc gcgctgaccg gatcgaccgc
      541 gaccacgccg aagccttcca tcttgcccat ataggcgagg accgcgacat cgccgctgcg
      601 accctcgccg aagcggaaat cgcggcccac cacgacgcag cccgcgccga ggccgttcac
      661 caagatgtcg agaacgaaat ccggcgcgct cttgtgcgcc agcgcggcgt cgaaggcgag
      721 cgcgaacatc gcatccacgc cgagcgcggc gatcaggtgc gacttgaggc ggaacggcgt
      781 caggcggaac ggctcgccgc cgggccggag gaattccctg ggatgcggct cgaagaccat
      841 cacggccagc ggcgcgcccc gctcttccgc cagacggcgc gcctcgccga tcaaggcctg
      901 gtggccgaga tgcacgccgt cgaaattgcc gacggcaatg acggcgccct tgtaggcctc
      961 cggcacgtcg gcgatgtgac ggaaaatcct catcgcgccg tcaggccgcc tctcgcaaag
     1021 gcaccatgac cgtcgcctcg ccgctcacaa ccaccgtatc cttcaccttg cagaggcaat
     1081 cgaagatcac gcagcggctt gcgaggtgca ctttgcgcac cgtgcagacg gcggtgaccg
     1141 tgtcgccgat ccgcaccggc gccttgaagc gcgtggtctg gctcaaaaag accgcgcctg
     1201 gtccgggcat cttggtgccg agcaccgtgg agagatagct caacaccaag gctccgtgga
     1261 cgatgcggcc cttgaagacg gtggtcttgg cgaaatcctc gtcgaaatgc gccggattga
     1321 ggtcgcccga aacctcgccg aacagcgcca cgtcgcgttc gctgacggtt ttagcgaagc
     1381 tctcggacat gccgggcttc agttcctcga tggtatacgt catgcggtgc gaatccttcg
     1441 cgatctatga ggagtagcac cctaccacgc aagcgcgccc attgccgcgc gcgcgtatac
     1501 gcccttcttg gcgaacagcg cggcaagatt ccccggcgtt aatgcgccga cctcctcgcc
     1561 atgcacgccg tcggcgatga acagcgcgtc gatgccgaga cgattggcgc cgagcatatc
     1621 ggtttccaat ccgtcgccga ccaccagcgg gtgcttgggc gaccccgcgg cctcgagcgc
     1681 aaccttatat atgggcagat gcggcttgcc gtaatagacg acctcgccgc cgatctcctc
     1741 gtaggcgcgc gccaaggcgc cggcgcaata aagcagcttg ccgccgcgcg gcacgaagat
     1801 gtcgggattg gcgcagacca tgggcagttt gcggtttttc aattccgcga gaacgccgcg
     1861 gtaattttcg ggcgtctcga tttcgtcgtc gtacagcccg ctgaccaacg cgaccgcagc
     1921 cttctccggc ccgaccagtt cgacgttcag cccgtcgtaa agaggacgat cgcgttcggg
     1981 gccaatgtgc atcatcgcga gggagcccgc ggcggcgcgg cgctcgatat cctcgcgcgc
     2041 ggcggcgccg gaggtgacga tggcgtcgta gcacccggcc ggcacgccga tctgggtaag
     2101 ctgctgctcg ataggggcgg acgggcgcgg cgcgttggac aacagcacga cgaggccttg
     2161 ttcggcgcga aagcgccgca gcgcctcgac ggccgccaaa tgcgggtgca ggccgtcatg
     2221 catcacgccc cagacgtcgc agatcagcgc atcgtagccg ccggcgatct cgcgaaggcc
     2281 ggaaatgatg gtgggaacag aggtcatcgg ctggagtcgt tttgtatcgg ggttgctcca
     2341 cttcgtcatg gccaccaccc cggtccgcgc gaagcgcggc cggagtgcaa gctcttgagg
     2401 cgtccatcca gttttagagt gtccccgtaa tgaacggtta cgctggatgg ccggggcaag
     2461 cccggccatg acgaaattga agattcagtt cattccaaat ccgctctagc ggttagaggg
     2521 acgcgcgcgc gcggtcaata ggtttatccc gcccgccgga aaggaataac cggcgcccgc
     2581 gtttccgcct tctcgacagg ctttatgaca tcgtcgcgca ccgcgcgcgg ctcgccgaac
     2641 agatagccct gggcgaaatc gaccgcataa tccagcaatt gcaggacggt cttttcctct
     2701 tccacacgct cggcgatcag gttgaggccg tggcgctcca acagcttctt gaagtcctcg
     2761 gcggcaacgg cggcctgggc cgtgcccatg ccctgggtca gggtttcggc gcgcaccttc
     2821 aagtgcttga agcccatgtt cttcaatttc tcgaaattga gcgccagggt ttccacatgg
     2881 tccatggaga gcgcgaagcc cagcccgctc agatagttca gattggcttc gccttccacg
     2941 ccggccttca gcaccgcgct ctgttcgaac tcgaacacga tctggcccgc caagtcgcgg
     3001 ttgttgtgca tatattccag gaactgcggg aagaattcgg cgtcgcgcag cgtgtcgccg
     3061 gaaatgttgc agaagacgcc gatgccgcgg ttcttgctgg tcagcttgcg cacgatctgc
     3121 acgcagcgga acagcagcag attgtccacc accgacatca agcccgcggg cgcggccacc
     3181 ttgatatatt gcgccggcat gatcaccgcg ccgtcttcgg cgcgcaggcg cgacaacgcc
     3241 tcgtagtagc gcagctttct ttgcggcagg ctgacgatgg gctggaggta caaatcgacg
     3301 cggttctctt cgagcgaggc gcggatggtc tccagcagct ccggctcggc cagcccttcg
     3361 agcgtggcgg gcgccggagc gcgtacgcgg cgctcggcga agctctcctc ggcgggctcg
     3421 gccttcgcct tgtgggaaat cctgcccgcg aactcgcgca tcaggctttc gagcacttgc
     3481 agttcggaaa cgatcttctt gccttgggcg gaggcgcgcg cttcgaccgc cttggtcacc
     3541 tcgtccatct tggcgcgggt ttcggccacc gccttttcca tggccaaatt ggcgcgcttg
     3601 aggttcacga tctcggacct gtgcgccttg cggaagcggc gctgcgccag cgcggagtcc
     3661 gcctgcaaca tcgccaagag ggcgatcagg ccgcagagaa tgccggtgga cagccccaga
     3721 tacagaccgg ccaaactgcc gagcgtgagg ctcgccagcc cgtaaatgac gttgaggagg
     3781 atcctgacca tgggacggtt ccggcgcttt ggaccggcaa caatcgccta gtgcccgttt
     3841 ccaatccgtt aaccaaggcg ccaaaacaca aggcgggctg cgcacggacg gccgtttccc
     3901 ggcccccggc gaacccgcct tggtttgaag ctacggccgg caagtggaat ccgcgaggcg
     3961 aaaaccgcaa gcacctttgc tttgcctcag gcttcgcgga gcgagcgttc tgccgcgcga
     4021 ccgccccttg ggccaggccg cttcgccggc ttgctctttg cggatgcgcg cgagtccgca
     4081 cccaagccct gtatggtctc gcgtcagctt cggatcggaa ccttgagacg gcctcgtggc
     4141 acaggacgga acatgacttc tccctttcga tcccgcggaa cggacggcct gggccgcttc
     4201 cccgacgggt agcgatggga acgaaatggg aacttgttac gggaattcaa ggggacgcgg
     4261 aagatttaat gttagagaat gggagccatt atcatctgtg tacggcgtgg tcggtcggac
     4321 cactcaatct ctgcaattcg tatcccgtcg ccaaatttgt ctcagtgccg ttgccggtgt
     4381 tgctcccaaa atttagtcta gctctaaccc caattcttcg ccgact
//
LOCUS       DASWPU010000467         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_114524,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000467 DASWPU010000000
VERSION     DASWPU010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114524"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VGC27_09905"
     CDS             complement(<1..357)
                     /locus_tag="VGC27_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282923.1"
                     /translation="MNSTLAALVALAFAAAAFGEQYRIQVQGVTTALSAGSGSWGDDV
                     AIGTPLEIAFTIDPVSAFPFNSTATNYQGTDGLLTVGSFRMQMRTNYFVFNDSPSFGD
                     GYRTGSIFPATNNRFPY"
     gene            complement(551..2035)
                     /locus_tag="VGC27_09910"
     CDS             complement(551..2035)
                     /locus_tag="VGC27_09910"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEY0282924.1"
                     /translation="MPPAEQLYQETLALQARVADLEAQVAWFRRQVFGQRSEKLVVDS
                     PTQAKLGLPETPRIEPATHTVSYERRTPAPEKRPIPAEVFKDLPVAETIELLPEEVQA
                     EPEAFERISEESTFEVDLIGPRLVKRVIVRPKFRRKADRNQAPVIAPALPRPALGGYA
                     AAGLLAFIAVSKYQHHLPLYRLEAMSPQWGAQLSRKSMADWIRLVADWAEPIYKLMLR
                     ELIAHHYLQCDETPVKFLDPDEKGRGAIQGYLWVVSAPGGDVVFDWRLTRRHGELTTL
                     LTDDYAGLLQSDGYEAYAAYAEKHPAVTWLGCWAHARRYFFEAQGDNRRIAQAALRLI
                     GRLYRREREWDEQKLSPEQRGAERAKPEGLARTMDSLKRLAVWARQRVRPKSPLGKAC
                     DYLLGQWEPLSAHREHGESRLDNNLVENAIRPSCIGKKNWLFIGHPEAGQRSAILYSL
                     IVSCQRHGKDPLAYLKDILARLPRLTNQDDLRQLTPRLWQPTTS"
     gene            complement(2088..2429)
                     /gene="tnpB"
                     /locus_tag="VGC27_09915"
     CDS             complement(2088..2429)
                     /gene="tnpB"
                     /locus_tag="VGC27_09915"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY0282925.1"
                     /translation="MLSLSPTQRIFLALEPVDMRKHFDGLWALASEKLQEDPFGGAWF
                     VFTNKDRNRLKILCWDGSGVWVLAKRLEKGRFSWPQGAGAKLDLEAAALTMLLSGIDL
                     KDGCKKAWYQR"
     gene            complement(2434..2778)
                     /locus_tag="VGC27_09920"
     CDS             complement(2434..2778)
                     /locus_tag="VGC27_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282926.1"
                     /translation="MTTITAEVVKAEGKRARDGRTITPAARRAELVRGYRSSGLTMAQ
                     FAQREGIKTTTLAKWVYLSDHCSGRPPVRFAEVKLPAVAPWAFELTLPNGWVVRAADA
                     AALAALLNVAGR"
BASE COUNT          475 a          949 c         1010 g          544 t
ORIGIN      
        1 gtagggaaac cggttgttgg tcgccggaaa gatgctgcca gttctatagc catcgccaaa
       61 ggagggactg tcattgaaga cgaaatagtt tgtgcgcatc tgcatccgga acgagcccac
      121 cgtgaggagc ccgtcggtgc cctggtaatt cgtggcggtg ctattgaacg gaaaggcgct
      181 cacgggatcg atcgtgaacg cgatctccaa tggcgtgccg atcgccacgt cgtcgcccca
      241 cgagccggag ccggcgctca acgccgtggt cacaccctgc acttgaatcc ggtactgttc
      301 gccgaaggca gccgccgcga atgccagcgc caccaacgcc gcgagggtgg agttcaacgt
      361 tctcgtcttc ataaaatggg tccggtctgt cgttgcctgt ccgtcttctg tcgtcagagg
      421 tctgtcatcc gtcgtccgac taccgtaagc gtccggccgg cgacctactg gggcggcgtt
      481 gctgaagact gaagcgttgt ctcaatggtg cgcattgaga cggcgtaatg agcaccaagt
      541 ggaagtcgca ctacgacgtg gtcggctgcc agaggcgtgg agtgagctgg cgcagatcgt
      601 cctggttggt caggcggggc agccgggcga ggatgtcttt gaggtaggcg agcgggtcct
      661 tgccgtggcg ctggcaggag acgatcagcg agtagaggat ggcggagcgt tgaccggcct
      721 cggggtggcc gatgaacagc cagttcttct tgccgatgca ggaggggcgg atggcgtttt
      781 cgacgaggtt gttgtccagc cggctttcgc cgtgttcgcg gtgtgcggac aacggttccc
      841 actggccgag caggtaatcg caggccttgc ccaggggcga cttggggcgg acgcgctgcc
      901 gggcccacac ggccaaccgc ttcaagctgt ccatcgtgcg ggccagacct tcgggtttgg
      961 cgcgttcggc cccgcgttgc tcgggcgaga gcttctgttc gtcccactcg cgttcgcgtc
     1021 ggtacaaccg gccgatcaag cgcagggcgg cctgggcgat gcggcggttg tcgccttggg
     1081 cctcgaagaa gtaccgccgg gcatgcgccc agcagcccag ccaggtcacg gccgggtgtt
     1141 tttcggcgta ggccgcgtag gcctcgtagc cgtcggactg caacagcccg gcgtagtcgt
     1201 cggtaagcag agtggtcagt tcgccatggc ggcgggtcag tcgccagtcg aagacgacgt
     1261 cgccacccgg ggcgctcacg acccagaggt agccttggat ggcgccgcgg cccttctcgt
     1321 ccgggtcgag gaacttcacc ggagtctcgt cgcattgcag gtagtggtgg gcgatcagct
     1381 cccgcagcat cagtttgtag atgggctcgg cccagtcggc gaccaaccgg atccagtcgg
     1441 ccatgctctt gcggctgagc tgcgcgcccc actggggcga catcgcctcg agccggtaca
     1501 gcggcagatg gtgttgatac ttggagacgg cgatgaaggc cagcaggccg gccgccgcgt
     1561 agccgcccag ggccggacgc ggcagcgccg gggcgatcac cggagcctgg ttgcggtcgg
     1621 ccttgcggcg gaacttcggg cgcacgatca cgcgtttgac caagcggggg ccgatgaggt
     1681 cgacctcgaa ggtggattcc tcgctgatgc gttcgaacgc ctccggctcg gcctgcacct
     1741 cctcgggcaa gagctcgatc gtctcggcca ccggcagatc cttgaagacc tcggccggga
     1801 tcggccgttt ctccggcgcc ggggtgcgac gctcgtagga gaccgtgtgc gtcgccggtt
     1861 cgatacgcgg ggtctccggc aggccgagct tggcctgtgt cggagagtcg acgacgagct
     1921 tctcgctgcg ttgcccgaag acctgccggc ggaaccaggc gacctgcgct tcgaggtcgg
     1981 ccacccgcgc ctgcagggcg agggtctctt ggtagagttg ttcggcgggt ggcatcggct
     2041 cggtggatca gcacgacgaa tgccacaggt tgttgcatgc caatatatta tcgctgatac
     2101 catgcttttt tgcagccgtc cttcagatcg atgcccgaga gcagcatcgt caacgccgcc
     2161 gcctccagat cgagcttggc gccggcgccc tgcggccagc tgaaccggcc cttttccagc
     2221 cgcttggcca gtacccagac tccggagcca tcccagcaga ggatcttgag ccggttgcgg
     2281 tccttgttgg tgaacacgaa ccaggcgccg ccgaagggat cctcctggag cttctcgctg
     2341 gccagggccc acagcccgtc gaagtgcttg cgcatgtcca ccggctcgag ggccagaaag
     2401 atgcgctgtg ttggcgacaa cgagagcatc gcgtcagcgc cccgccacgt tgagcaacgc
     2461 cgccagcgcc gccgcatccg ccgcgcgcac cacccagccg ttgggcagcg tcagctcgaa
     2521 ggcccacggc gcgaccgccg gcagcttcac ctccgcgaac cgcaccggcg ggcgcccgct
     2581 gcaatggtcg ctcaaataca cccacttcgc caacgtggtc gttttgatcc cttcccgctg
     2641 ggcaaattgc gccatcgtca gtccgctcga ccgatagccg cgcaccaact ccgcccgccg
     2701 cgccgccggt gtgatcgtcc gcccatcccg ggcacgtttc ccctccgcct tcaccacctc
     2761 cgctgtgatc gttgtcatcg ccacagccta ccagctgcgc gatctctcga ctacggggtc
     2821 gccggtcgga cgcttacctg gcaggtgatg agcagcgagt agaggatggc gaggcgaggg
     2881 ccggcgtcgg gatgaccgac gaacagccag tttttcgctc cgagcttgct gggacggatg
     2941 gcgttctcga cgaggttgtt gtcgagccgg gtgcggct
//
LOCUS       DASWPU010000468         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_120598,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000468 DASWPU010000000
VERSION     DASWPU010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120598"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VGC27_09925"
     CDS             <1..860
                     /locus_tag="VGC27_09925"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY0282927.1"
                     /translation="IRPRRSVLYMPGSNARALEKARTLPADALILDLEDAVAPDAKEA
                     ARAQVCAAVRAGFGRREVAVRINALSTPWGAKDLLAAAAAKPDAILLPKVSAPEDLHT
                     IEQRLAGIDPSLALWAMVETPLAILNIATLAGAGGRLACLVLGTNDLIKDMRAVHTGD
                     RLNLAAALGLSVLAARAHGLAVIDGVYHDIADIEGFAAACAQARSFGFDGKTVIHPSQ
                     IVPCNAAFAPSPEEVETAKRIVAAFELPENKGKGAIKLDGRMVERLHAEDARRTVALA
                     QAIGALGAD"
     gene            932..1333
                     /locus_tag="VGC27_09930"
     CDS             932..1333
                     /locus_tag="VGC27_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282928.1"
                     /translation="MFWNLFTTTAFVIAGLLFYHFWPRIFAALKRFDDQNRARIESER
                     RDRRDNLAHFRHTLSIAEEQVEAVGEIEVPDERTAVPVKRYLFEGERFALKREAERAR
                     EDKVRALARSFYMELPAALAARKGDGKLGKD"
     gene            complement(1341..>1871)
                     /locus_tag="VGC27_09935"
     CDS             complement(1341..>1871)
                     /locus_tag="VGC27_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282929.1"
                     /translation="TGAQAETLTQFFGASDLTGLAKDTTVLNLEAQSASKTAQTDVNA
                     FDDFFLINGAGKLVVNFDRALHNKGEAAIPGWVGVARYSNHVIKSDIQAGYEAVKAQY
                     KLTNALGHVTVYKTLNTGQLVYDYAVELSPGSAVCQEYLYLPATKAFQLGMTVGCYWT
                     LDSFQPHGFGSAGKPK"
BASE COUNT          326 a          615 c          609 g          321 t
ORIGIN      
        1 cgatccgtcc gcgccgcagc gtgctttaca tgcccggctc caacgcgcgc gcgctcgaaa
       61 aggcgcgcac gctgccggcg gatgcgctga tcctcgacct ggaggacgcg gtcgcccccg
      121 acgccaagga ggcggcccgc gcccaggtct gcgccgcggt gcgtgcaggc ttcggccggc
      181 gcgaagtcgc cgtgcgcatc aacgcgctgt ccacgccgtg gggcgccaag gatctgctcg
      241 ccgcggccgc cgccaaaccg gacgcgatcc tgctgccgaa ggtttcggca cccgaagact
      301 tgcacactat cgagcaaagg cttgcaggta tcgacccctc gctcgctctg tgggctatgg
      361 tggaaacgcc gctggcgatc ctgaacatcg cgacgctggc gggcgccggc gggcggcttg
      421 cctgtctggt gctgggcacc aacgatctga tcaaggacat gcgcgcggtc cataccgggg
      481 accggctcaa tctcgcggcg gcgctggggc tttcagtgct ggcggcgcgc gcgcacggac
      541 tggcggtcat cgacggcgtt tatcacgaca tcgccgatat cgagggtttc gcggccgcct
      601 gcgcgcaggc acggagtttc ggcttcgacg gcaagaccgt gatccatccg agccagatcg
      661 tgccttgcaa cgccgccttc gcgccatcgc cggaagaggt cgaaacggcg aagcggatcg
      721 tcgccgcctt cgagctgccc gaaaacaagg gcaaaggcgc gatcaaactc gacggacgca
      781 tggtcgaacg gctgcatgcc gaggacgcca ggcgcacggt ggcgctggcg caagcgattg
      841 gcgcgttggg cgccgattag ccaggcgctg cggcggctac acgaaatccg tgacccggcc
      901 tatactcgca aggcaatttg ccgggttctg catgttctgg aatcttttca ccaccaccgc
      961 cttcgtcatc gcgggcctgt tgttttatca cttctggcca aggattttcg ccgcgctcaa
     1021 gcgcttcgac gaccagaacc gcgcccgcat cgaaagcgaa cggcgcgacc gccgcgacaa
     1081 tctcgcccat ttccgccaca cgctcagcat cgccgaggaa caggtcgaag cggtcggcga
     1141 aatcgaagtg ccggacgaac gcaccgccgt tccggtcaag cgctatctgt tcgaaggcga
     1201 acgcttcgcg ctcaagcgcg aggccgaacg cgcgcgcgag gacaaggtgc gggcgcttgc
     1261 ccgcagcttc tatatggaac tgcccgccgc ccttgcggcg cgcaaaggcg acgggaagct
     1321 cggcaaggac tgaccggcgt ctatttcggc ttgccggccg aaccgaagcc gtgcggctgg
     1381 aagctatcga gcgtccaata gcagcctacg gtcatgccga gttggaacgc tttcgtcgcc
     1441 ggcaggtaaa gatattcctg gcacaccgcg ctacccggcg acagctcgac cgcatagtca
     1501 taaaccagtt ggccggtgtt gagcgtcttg tagacggtga cgtgcccgag cgcgttcgtc
     1561 agcttatact gcgccttcac agcctcgtag cccgcctgga tgtcggattt gatcacgtgg
     1621 ttggaatagc gggcgacgcc cacccagccc ggaatggccg cctcgccctt gttgtgcagg
     1681 gcgcggtcga aattgacgac gagtttcccg gccccgttga tgaggaagaa atcgtcgaac
     1741 gcgttgacgt cggtctgcgc cgtcttggac gcgctttgcg cctccagatt gagcaccgtc
     1801 gtatccttgg cgaggccggt cagatcgctg gcgccgaaaa actgagtcag cgtctcggcc
     1861 tgtgcgccgg t
//
LOCUS       DASWPU010000469         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_108390,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000469 DASWPU010000000
VERSION     DASWPU010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108390"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..3522)
                     /locus_tag="VGC27_09940"
     CDS             complement(109..3522)
                     /locus_tag="VGC27_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEY0282930.1"
                     /translation="MNDIARQIWDMKYRLKQPDGTPVDDDVAASWARVALAASGAEAP
                     EVRRDHAIAFAKALAGYRFLPAGRILAGAGTGRSVTLFNCFVMGAIPDSMDGIFSHLR
                     EAALTLQQGGGIGYDFSSLRPKGAPVAGVGADASGPVSFMDVWDAMCRIIMSAGSRRG
                     AMMATLSCDHPDIEDFIDAKRTPGRLSMFNLSVLATDRFMAAVKDDAPWELKFGGRVM
                     KTLPARDLWNRIMRSTYAYAEPGVIFIDRINRQNNLGYCETIRATNPCLTGDSWIMTR
                     EGARQIQDLVGRPFTAIANGAPWPSDPRGFFSRGIKPVVELATCEGYKLRLTSDHRLR
                     KIVRRTRWSCEWQWAEAASLTAGDEVQLHDHRALPSWGNAESSHQDNAEGYLLGLLIG
                     DGVLKADKAVLSVWPGKRVVNGEDERPGVRGIMERALAAAQTLPHRADFSGWIEVPGR
                     GEYRLSLGALKHLAKAHGLSPRNKNVTPEIERRSSSFTRGFLQGFFDADGSIQGNQAK
                     GVSIRLSQSNLARLTAVQRMLLRLGIESTIYTDRQAAGLSLLPDGKGGRKAYKTEAQH
                     ELSIAGENIALFAERIGFSEQEKAHRLKQLLAGYKRHLNHASFFATVRSVAPAGETEV
                     FDAIIPGLNAFDANGLWSHNCGEQPLPPYGACLLGSINLAKLVKTPFEDNAELDLAAL
                     AELTRTAVRFLDDVIDISRFPLEAQRAEAFAKRRLGLGVTGLADALIFCRAKYGSPES
                     IDLIERWLATLSHAAYRASVELAREKGAFPLFDAVEYLKRPFIEALPDDIRAGIAEHG
                     IRNGLLTSIAPTGTISLFAGNVSSGIEPVFAYDYSRRVLLPDGSSREETVSDYAVEAF
                     RARFGQTALPDYFVSAQTLAPEDHLAVQAAAQKYIDSSISKTINVPATISFDAFKDIY
                     TRAYELGCKGCTTYRPNTVTGSVLAAPAPEESDEPPLPMELPVLRESGGIVYMTKPLD
                     RPDVLLGQTYKIKWLDADHAFYITINDVEKDGRRRPFEIFINSKNMEAYAWTLALTRM
                     ISAVFRRGGDVSFVVEELKAVFDPRGGQWMEGHYVPSLLAAIGEIVERHLIAIGFIAS
                     PGGGEEEKRAMAAAAEGAPARHCPRCGSPSFVKLEGCDSCLSCGYSKCA"
BASE COUNT          640 a         1106 c         1159 g          692 t
ORIGIN      
        1 cacccaccac tggatggcgg cgcccagcgc gacggcgagg caaaggccca gcggcgcata
       61 aaggaagaag cggctcgaat atttcatgcg ggggctattt tagcccttct aggcgcattt
      121 cgaatagccg caggaaaggc aggaatcgca gccttccagc ttgacaaagc tgggagagcc
      181 gcagcgcggg cagtgtctgg cgggcgcgcc ttccgccgcc gccgccatgg cgcgcttttc
      241 ttcttcgccg ccgccgggtg aggcgatgaa gccgatggcg atcaggtggc gttcgactat
      301 ttcgccgatg gcggccaaga gcgagggcac gtaatggcct tccatccatt ggccgccgcg
      361 cggatcgaac accgctttca attcctccac cacgaaagag acgtcgccgc cgcggcggaa
      421 cacggcggaa atcatgcggg tgagcgccag cgtccaggca taggcttcca tgtttttgga
      481 gttgatgaag atctcgaacg gccggcggcg gccgtctttt tctacgtcgt tgatggtgat
      541 gtagaaggcg tggtcggcgt ccagccattt gatcttgtag gtctggccga gcagcacgtc
      601 gggccggtcg agaggcttgg tcatatagac gatgccgccg gattcgcgca gcactggaag
      661 ttccatgggc agcggcggct cgtcgctttc ctcgggggcg ggcgcggcca gcaccgatcc
      721 ggtcactgtg ttggggcgat aggtggtgca gcccttgcag ccgagttcgt aagcgcgggt
      781 atagatgtcc ttgaaggcgt cgaaggaaat cgtcgccggc acgttgatgg tcttggaaat
      841 cgagctgtcg atgtatttct gcgccgccgc ctggaccgcg aggtgatctt ccggcgccaa
      901 ggtctgggcg ctgacgaaat aatcgggcag cgccgtctgg ccgaagcggg cgcggaaggc
      961 ttccaccgca taatcgctga ccgtctcttc gcggctgctg ccgtcgggca ggagcacgcg
     1021 gcggctataa tcgtaagcga agacgggttc gattccgctc gaaacgttgc cggcgaacaa
     1081 ggagatggtt cccgtcggcg cgatggaagt gagcaggcca ttgcggatgc cgtgctcggc
     1141 gattccggcg cggatatcgt cgggcagggc ttcgatgaac ggccgcttga ggtattccac
     1201 ggcgtcgaac agcggaaagg cgcctttctc acgcgccagc tccaccgagg cgcgataagc
     1261 ggcatggctc agcgtcgcca gccatcgttc gatcaggtcg atgctctcgg gcgagccgta
     1321 tttcgcgcgg cagaagatca gcgcgtcggc gaggccggtg acgcccagtc cgaggcgccg
     1381 cttggcgaag gcttcggcac gctgggcttc cagcggaaag cgcgaaatgt cgatcacgtc
     1441 gtcgaggaag cgcaccgcgg tgcgcgtcaa ctccgccagc gccgccagat cgagttcggc
     1501 attgtcctcg aacggggtct tcaccagctt ggccagattg atcgagccca acaaacaggc
     1561 gccataaggc ggcaagggct gctcgccgca attatgggac cacaacccat ttgcatcgaa
     1621 ggcgttgagc cccggtataa tggcgtcgaa gacttcggtt tcgcctgcgg gcgctacgga
     1681 tcgcacggta gcaaagaacg aggcgtgatt gaggtgccgt ttgtagccgg ccagaagctg
     1741 cttgagccga tgcgctttct cttgttcgga gaagccaatc cgttccgcaa acagtgcgat
     1801 attctcgccc gcaatgctca gttcgtgctg cgcctcggtc ttgtatgctt ttcttcctcc
     1861 cttgccgtcg ggaagcaggc ttaatccggc cgcctgccgg tccgtataga tcgtactctc
     1921 gattcccaag cgcaggagca tgcgttggac ggcggtcaag cgcgcgagat tgctttggct
     1981 caggcgaata ctgacgcctt tcgcctgatt gccctgaatg cttccgtccg cgtcgaaaaa
     2041 tccttgcagg aatcctctgg tgaaactcga cgagcggcgc tcgatctccg gcgtgacgtt
     2101 cttgttacgc ggagacaaac cgtgcgcctt cgccaaatgc ttcagggcgc cgagcgaaag
     2161 gcgatattcg ccgcgccccg gtacctcgat ccagccggag aagtcggcgc gatgcggcag
     2221 ggtttgtgcc gccgcaagcg cgcgttccat gattccgcgc acgcccggcc gctcgtcttc
     2281 gccgttcacg acacgcttgc ccggccaaac cgaaagaacg gccttatccg ccttgagcac
     2341 gccgtcgccg atcagaaggc ccagcaggta accttccgca ttgtcctgat gactgctttc
     2401 cgcatttccc cacgagggca acgcgcgatg atcgtggagt tgcacctcgt cgcctgcggt
     2461 gagtgacgcc gcctccgccc attgccattc gcaagaccag cgtgtccggc gcacgatctt
     2521 gcgcaggcgg tgatcgcttg tcagccgtaa cttgtaaccc tcgcaagtcg cgagttcgac
     2581 aaccggcttg atcccgcgcg agaagaagcc gcgtgggtcg ctcggccaag gcgcgccgtt
     2641 cgcgattgcc gtgaaagggc ggccgaccag atcctggatt tgccgggcgc cttcccgggt
     2701 catgatccag ctatcgcccg tcaggcacgg attggtcgca cggatggttt cgcagtagcc
     2761 gagattgttc tgccggttga tacggtcgat gaagatgacg cccggttccg cataggcgta
     2821 ggtcgagcgc atgatgcggt tccacagatc gcgggcgggc agcgttttca ttacccgtcc
     2881 gccgaacttc aattcccagg gcgcatcgtc cttcaccgcc gccatgaaac ggtcggtcgc
     2941 cagcaccgag agattgaaca tggaaagccg gcccggcgtg cgcttggcgt cgatgaagtc
     3001 ctcgatgtcg ggatgatcgc aggagagcgt cgccatcatg gcgccgcggc gcgatcccgc
     3061 gctcatgatg atgcggcaca tcgcgtccca cacgtccatg aacgagaccg ggccggacgc
     3121 atccgcgccc acgcctgcca ccggcgcgcc tttgggcctg agggaggaaa agtcgtaacc
     3181 gatgccgccg ccctgttgca gcgtcagcgc cgcttcgcgc aggtgcgaaa aaatgccgtc
     3241 catggaatcg ggaatggcgc ccatgacgaa gcagttgaac agggtgacgc tgcgcccggt
     3301 gccggcgccg gccagaatcc ttcccgctgg caggaagcga tagcccgcca gcgccttggc
     3361 gaaggcgatg gcatgatcgc ggcgcacctc gggcgcctcg gcccctgaag ccgccagcgc
     3421 cacgcgcgcc cagcttgccg ccacgtcatc gtccacgggc gtgccgtccg gctgtttcag
     3481 ccggtatttc atgtcccaga tctggcgggc gatgtcgttc atggcttcaa tctagcattt
     3541 ggtggttgac cggttctgac ccccggaatt attttttgtt catgtattgt tctaggc
//
LOCUS       DASWPU010000470        12314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_47037,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000470 DASWPU010000000
VERSION     DASWPU010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 12314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12314
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47037"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VGC27_09945"
     CDS             <1..664
                     /locus_tag="VGC27_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEY0282931.1"
                     /translation="WRQKGKKAIQIGLLGALNGLRLRAWYEGESTADVVDTCRRLDIP
                     VFETDVTNSDETVRLMVGAGADLGLSLGNGYIAPRVFGVPKFGMINVHGERLPEYQNA
                     QSVIWPIYNLETTTGLTIHQIDRSIDTGRILYREEYPIVFRARLEDTVRATMEITRKR
                     TPAALRHVCENYERLSAEAIPQKKGHKYTTPNIRQFIRMTRNNRRLYNKIEHAESEHN
                     GH"
     gene            716..1750
                     /locus_tag="VGC27_09950"
     CDS             716..1750
                     /locus_tag="VGC27_09950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0282932.1"
                     /translation="MRMRALERLGHEVRGVDTVEPLRRASWLGRHIRRRLQLDSVVGD
                     INRSVVAAARAFRPQIVWGEKQEFLRVETIAELHKLGATMVHFTPDPYFSLYWKRTRL
                     MDAAVGAFDILVYCKSYERKAYEALGKPLIYMPLGYCDEVHRPLPSDDPRWRCAVGFL
                     GGWEPRREHLLHAVAAAGVGLKIWGGYWQFLRDGKWTLRRHIVLRQLAGGDPFRIHRD
                     ELLTAAWQGREVYADDYARALTGSGIGLGFLRKVCPDQHTTRSFEIPACGSMLLADRT
                     DEHREFFEEGKEAEFISSVEELLEKLTFYCANDAARERIARAGRERCLTGRYAYVHRL
                     ATVLEALKQV"
     gene            complement(1737..2099)
                     /locus_tag="VGC27_09955"
     CDS             complement(1737..2099)
                     /locus_tag="VGC27_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282933.1"
                     /translation="MARQRGQAVDSGIGADIQNNLGASCRDFPARENFVFQKIRSKKI
                     ELALQHFVQIAAHLEAAMAQARKPLGAAAFGRQTPKRVDRPHLASADGKMPRIGNEKI
                     FQRGHLRRKHGGVDPKLV"
     gene            2079..2639
                     /locus_tag="VGC27_09960"
     CDS             2079..2639
                     /locus_tag="VGC27_09960"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0282934.1"
                     /translation="MAALASQPDAAVHAFEPTPEIAARLRETAKLNRLDRLYVHEAAV
                     SNRSGQATLKRWRGETGSNEGMNFISTDASEPDAEHVETVCLDQFCADNAIERIDLMK
                     IDIQGNEPQAFAGAARLLRERRIGTVFAELNWAPGRREEPATQVIETLEEAGFQFARP
                     AVRPEWRKSGDWMRKLSDIVAQRSPG"
     gene            2702..4000
                     /locus_tag="VGC27_09965"
     CDS             2702..4000
                     /locus_tag="VGC27_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282935.1"
                     /translation="MLLWVLRKDSVSFGLPFAYLSLLLLQHVPGAYVQLADERFIGHR
                     HEIEIGIRLTAIGVVCFLIGAWLARARTAEARVTSVQPTCMTRFEEDRPFWLFCFFGG
                     MLFTFGLTPLRNLPSIGAIVYNGGVIWVLGVLLGLRAAIRKRDTTWIMVWGALLVAYP
                     ALILFAMGFLSYGAQAVIIVVCALAISGRRYWRVVITTVLTVYLALSLFSNYFQARDN
                     IRDAVWSGASTERRLDAISKVFTEFKFFDGTDELVLVGFDLRLNQNYFIGLASENIDN
                     GSVKFLYGESITDAMLALVPRALWPGKTVFGGSPEVVSNMTGLELSPNTSWGVGNVME
                     FYINFGVPGLIIGFLGLGWLLGRFDHRAALAETRHDYGTTLLFFLPAVALVQPLGSMV
                     ELSGSMGAAWLAAYLWKWVWAHWDHRDRGEPVVARDKPLS"
     gene            3997..5085
                     /locus_tag="VGC27_09970"
     CDS             3997..5085
                     /locus_tag="VGC27_09970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0282936.1"
                     /translation="MRIFLAATSLSPHYGGPAVSVSRLALALCGAGADVGLWAQDGSA
                     LTTALPPQQLPRGLTRLSGSAPEALKRFTPDILHDNGLWLRHNHRLASLASRRGLARL
                     VSTRGMLEPWARSHKKWKKDLAWAFYQRRDLSSAKAVHATAAREAENLARLNLGVPVH
                     TIPNGVDIPELPGRKSTGTDVPHRTAVFLGRLYPVKGLPLLVEAWNRVRPEGWRLVLA
                     GPDEAGHRAELEALIENAGLTGTITFAGPLAGPAKSQFLASAELFILPSYSESFGMAV
                     AEALAHGLPVLTTSGTPWPMLHRRGCGWTVDATVDGIAEGLSEATSHDSNTLAAMGAK
                     GRAWVASEFGWQGVAMQFLALYREISAH"
     gene            5191..6954
                     /locus_tag="VGC27_09975"
     CDS             5191..6954
                     /locus_tag="VGC27_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0282937.1"
                     /translation="MIILGLNAFHGDSSAALVRDGKLVAAVEEERFRRVKHWAGFPSQ
                     SIAYCLREAGLSLSDVDHVALNQDSSAHLLKKIAYVALHRPNPKLVLQRLGNRRKRKA
                     IPELLADAFPETAFRGKSHSIEHHLCHLFSAFYVSPFKDAVVVSVDGFGDFSSAAWGA
                     GHGNDITVDGRVFFPHSLGIFYEALTQWLGFPHYGDEYKVMGLAPYGKPAYMEQMRRI
                     VRLKPGGAFELDLRYFLHHREIVPYQWDDGSPEVGTLFSPAMEELLGPCRRPGAPLES
                     RHHDVARSVQAMYEEAFFHLITNLQKRSGLTDIGLAGGCAANSVANGKIRRQTRFERV
                     YVQSAAGDAGGAIGAAFALWRQLGGERSFVMDHAYWGTHFSNGDIEAFIEKNRSEIDA
                     GGCALENLPDEGELCRKTAQAIADGKVVGWFQGRMEWGPRALGNRSIVCDPRRADMKA
                     ILNAKIKRRESFRPFAPSILDDAVGEWFEEDDAVPFMMQVFQIREPQRARIPAVTHVD
                     GSGRLQTVYRHTNPRYYRLIEAFRDITGVPIVLNTSFNENEPVVCNPQEALDCFLRTK
                     MDVLVLGDVMMTRGGLARTVT"
     gene            complement(6995..8020)
                     /locus_tag="VGC27_09980"
     CDS             complement(6995..8020)
                     /locus_tag="VGC27_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY0282938.1"
                     /translation="MPPGSEGEVTKKSAIDLREMLQLVLNGWRIVAGAVVVFLLLAVI
                     NIHTSPSVFAVTMQVTPVQGSGDHAASRSNSLSSLAQLAGLNLSPSAGQSASQFRTYL
                     DSMHSRALADELAKNKDLMKSIFFREWDEKSQTWQKPQPGLVEVIKGGIMRLLGARPL
                     PWEQPDGARLQEVLNEPGTLEIHQDIKRPDLATIECDSSDPRFAIKLLNAVNQTADNL
                     LRKKALLRATQYIDYLSKELDTVTVAEHRQAIMQALSEQERFKMSASSAAPYAADVFD
                     GPWTSRKRLSPRPSQIYPVAILKGLLFGAAIVLVLEYFGAAIRSQIRNRLPVEKLPPF
                     LRRTLDS"
     gene            8792..10111
                     /locus_tag="VGC27_09985"
     CDS             8792..10111
                     /locus_tag="VGC27_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY0282939.1"
                     /translation="MTEFESKLSSKSIRVGVMGLGYVGLPLVHAFWQAGVTTVGFDKD
                     PGKIEKLARGESYIKHFAPEKIAAMIASGRFRATADFNELADVDAILICVPTPLAPTR
                     DPDLSYVVATTETIAKHVRAGTLVVLESTTYPGTTTEVVQPILEKSGLKIGSDILLAF
                     SPEREDPGNPIETRAIPKIVGGVDERSGVAAEKLYSMAFLKVHKVRDAQTAEAVKITE
                     NIFRSVNIALVNELKVVYSRMGINVWDVIEAAKTKPFGFMPFYPGPGLGGHCIPIDPF
                     YLSWQARAFDVDTRFIELAGEVNRAMPRLVVQTLAEALSMRASKALNGSDILIMGVAY
                     KKNIDDLRESPALHIMDIIKKLGGRVAYHDPYLPEIGPTREHASLQGMRSVPFDEATV
                     SKFDAALITTDHDNVDYAKLVSWSKLVIDTRNATKNVGAGKSKVVPA"
     gene            10640..>12314
                     /locus_tag="VGC27_09990"
     CDS             10640..>12314
                     /locus_tag="VGC27_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0282940.1"
                     /translation="MTVIGRPVGKLIFLILVAGLFSPRAYAQSAAPNLLQQLQQVQGQ
                     QGTNPGIDQGISQQTTVLVPLSPTNPQLPTSRLEQILSARAGVKLKQFGYDQLGVGRP
                     VSISQMGAVQDDYVLGPGDEIVVSLRGQENAEYRVTVDRDARVVLPRMNPISVGGRSF
                     GDFRRDLVAAIHRSYIATEGFISVGRVRQVSVLVSGEVNSPGVRTLTGLSTPADAILV
                     SGGIKKTGSMRNVYILRGSRRIAVDLYSVLTGHGVASHVSLGDGDRIVVPPLGPTVAV
                     AGWVRRPGIYEISGGRSSLNARDLLSLAGGLEVRGKYHLSVLRVAPDGRNRMSSLENE
                     SGAIGDSDILFVQPAADQTVNSATLSGGTALAGKFSITNTKLSQILKSPGALGGDPYT
                     LLGIISRRDPVALLRTLIAFTPVAVLKGSQDMDVQGDDIVRVISAKEARLMFATIRRY
                     EDLRQTSEEALRSPWLQSVQQQAYGQANATQQNSSSSQQTQYGGQQAPGAASTAGTPG
                     DQNSNPAATRYFTPVLSPDMATRQALLQAEDDQFSPGMVQSRLPNYQSSS"
BASE COUNT         2351 a         3559 c         3807 g         2597 t
ORIGIN      
        1 gtggcggcaa aaagggaaaa aagcgataca gatcgggctg cttggagcgt tgaacggctt
       61 gcgcctgcgg gcttggtacg agggcgagtc taccgcggat gtcgtggaca cgtgccggcg
      121 cctggacatt cccgttttcg agacggatgt cacgaacagc gacgagacgg tccgcctgat
      181 ggtgggagcc ggcgcggacc ttggtttgtc gctcggcaac ggctacatcg ctccgcgggt
      241 tttcggcgtt ccgaaattcg gcatgatcaa tgtccatggc gaacgcttgc cggaatatca
      301 gaacgcccag agcgtgatct ggccgatcta caatctggaa acgacgacgg gattgacgat
      361 tcatcaaatc gatcgctcga tcgacaccgg gcgaattctt tatcgcgagg aatacccgat
      421 tgtctttcgc gcccgcctgg aagacacggt gcgcgcgacg atggaaatca cccggaaacg
      481 cacccccgcc gccctccggc atgtctgcga aaattacgaa cgcctttctg ccgaggcaat
      541 cccacagaag aagggccata agtacacgac accgaatatc cggcagttca ttcgcatgac
      601 gcgaaataac aggcgtctat ataacaagat cgaacatgcc gaatccgaac ataacggaca
      661 ttgagtgacg aaaattctct ttcttggaga gatcagtccc gggcaaacgt cgcgcatgcg
      721 gatgcgcgcc cttgagcggc tcggtcacga ggtgcgcggc gtcgatacgg tcgaaccgct
      781 caggcgcgcg tcttggctcg gacgccatat ccgccggcgg ctgcagttgg attcggtcgt
      841 gggcgatatc aaccgttccg tcgttgcggc cgcgcgcgca ttccggccgc agatcgtgtg
      901 gggcgagaaa caggaattcc ttcgtgtgga aacgatcgcg gagctgcaca agctgggcgc
      961 gacgatggtc catttcacgc ccgacccgta tttcagcctg tattggaagc gcacgcgcct
     1021 gatggacgcc gcggtgggcg cattcgacat attggtctat tgcaagtcgt atgagcgcaa
     1081 agcgtacgag gcgctcggca agccgctcat ctacatgccg cttgggtatt gcgacgaggt
     1141 gcatcgtccg ttgccgtccg acgatccgcg ttggcgctgc gcggtaggtt ttctgggcgg
     1201 ctgggagccc cggcgcgagc atctgcttca tgcggtggcg gccgctggcg tcggccttaa
     1261 gatctggggc ggttattggc agttcctgag agacggaaag tggacgctgc gccggcacat
     1321 cgttctgagg cagcttgcgg gcggcgatcc gtttcgcatc catcgcgacg agcttctgac
     1381 cgctgcttgg caggggcgcg aagtctatgc ggacgattat gctagggcgt tgaccggctc
     1441 cgggatcggt ctcggctttc tgcgcaaggt ctgtcccgat cagcacacga cgcgcagctt
     1501 tgaaattccg gcctgcggca gcatgctgct ggccgaccga accgacgaac accgcgaatt
     1561 tttcgaagaa ggcaaggaag cggagttcat ttcatccgtc gaggaattgt tggagaaact
     1621 gacgttctat tgcgccaacg atgccgcgag agagcggatc gcccgcgccg gtcgcgaaag
     1681 atgcctcacg gggcgatatg cttatgtgca tcgactcgca accgtactcg aagcgcttaa
     1741 acaagtttag gatcgactcc gccatgtttc cgccgcaggt gcccgcgctg gaagattttc
     1801 tcatttccta tgcgcggcat tttcccgtcc gccgaggcaa gatgcgggcg atcaactcgc
     1861 tttggcgtct ggcggccgaa ggcggcggca ccgagcggct tgcgagcttg cgccatggcg
     1921 gcttcaagat gcgctgcgat ctgaacgaaa tgctgcagcg ccaattctat ttttttggaa
     1981 cgtattttct ggaagacgaa attctctctt gctgggaagt cgcggcaaga ggcgccaagg
     2041 ttgttctgga tatcggcgcc aatgccggaa tctacagctt ggccgcgctg gcgagccaac
     2101 cggacgccgc ggttcatgcc ttcgaaccga cgccggaaat tgccgcccgg ctgcgtgaaa
     2161 ccgccaaact taaccgcctc gaccgcctgt acgttcatga agcggcggtg tcgaaccgga
     2221 gcggacaagc gacgttgaag cgatggcgcg gcgagacggg atcgaacgag ggaatgaact
     2281 ttatttcgac ggatgcgtcg gagcccgatg ccgaacatgt ggagacggtt tgcctcgatc
     2341 aattctgcgc cgacaacgcg atcgagcgaa tcgatttgat gaagatcgac atccagggca
     2401 acgagcccca ggccttcgcc ggggcggcgc gcctgctgag agagcggcgt attggcaccg
     2461 tattcgcgga attgaattgg gcccctggcc gacgcgaaga acccgcgacc caggtgatcg
     2521 agacgctcga ggaggcgggc tttcaattcg ctcgtccggc cgtccggcct gaatggcgga
     2581 aatcgggtga ctggatgcgc aaactaagcg acatcgtcgc gcagaggagt ccgggctagc
     2641 catggaccca gacatccagt ccattcagat atcgctcgcc gtctgcattg cggcctattt
     2701 cgtgctgctg tgggtgctgc gcaaggacag cgtttcgttc ggtcttccct ttgcctatct
     2761 gtcgcttctc ttgctccaac atgtaccggg cgcctacgtg cagcttgccg acgaacgatt
     2821 catcggacac aggcacgaaa tcgaaatcgg gatacgtctt acggcaatcg gcgtcgtctg
     2881 tttcctcatc ggcgcctggc tggcgcgcgc gcgcacggcg gaggcgcggg taacctcggt
     2941 tcaaccgact tgcatgacac ggttcgagga agacagaccg ttttggttgt tttgtttttt
     3001 tgggggcatg ctctttactt tcggcctcac cccgttgcgg aacctgccga gcatcggcgc
     3061 tatcgtttac aacggcggcg taatttgggt tcttggtgtt ttgttgggac ttcgcgccgc
     3121 catccggaag cgcgacacga cgtggatcat ggtatggggc gccctgctgg tcgcttatcc
     3181 ggctctgatt ctctttgcca tggggtttct ctcttacgga gcgcaggccg tcattatcgt
     3241 cgtctgcgct ttggcaatca gcgggcgaag atattggcgg gtcgttatca cgaccgttct
     3301 gacggtttat cttgcgctca gtttgttctc caattacttc caggctcgcg ataacatccg
     3361 cgacgcggta tggtcgggcg cttcaacgga acggcgcctg gatgcgatct ccaaggtctt
     3421 taccgagttc aagttctttg acggtaccga cgaactcgtc ctcgtcggtt tcgatttacg
     3481 cttgaaccaa aattatttca tcggtctcgc gtccgagaac atcgataacg gcagcgtgaa
     3541 atttctttat ggcgaatcga ttaccgatgc gatgttagcg ctggttccgc gcgcgctttg
     3601 gccgggaaag accgtgtttg gcggcagccc cgaagtcgtt tcaaacatga cgggattgga
     3661 gttgagtccc aacacgtcgt ggggcgtcgg caatgtgatg gaattctaca taaactttgg
     3721 cgttccgggt ctgattatag gctttcttgg gttggggtgg cttctcggcc ggttcgatca
     3781 tcgtgcggcg ctcgccgaga cgcggcacga ctatggcacg actttgcttt tcttcctgcc
     3841 ggccgtcgcg ctcgttcagc ctctcggctc aatggtcgaa ttgagcggca gcatgggcgc
     3901 cgcctggctc gccgcctatt tgtggaaatg ggtttgggcg cactgggatc atcgcgaccg
     3961 aggtgaaccg gtggttgcac gggacaagcc gttgtcgtga gaatatttct cgcggccacg
     4021 agcctttcgc ctcactatgg cggaccggcc gtttccgtct cgcggcttgc tctcgcttta
     4081 tgcggcgcag gcgcagatgt tgggctgtgg gcgcaggacg gctcggcctt gacgacggcg
     4141 ctgccgcctc aacaactgcc gagaggtctg acgcgcctgt cgggttcggc gccggaggcg
     4201 ctcaagcgat tcacgcccga cattctccac gacaacggtc tttggctgcg ccacaatcat
     4261 aggctggcgt ccttggcgtc gcgtcgcggc ctcgcgcgtt tggtaagcac gcgaggcatg
     4321 ctggagcctt gggcgcggag tcacaaaaag tggaaaaagg acttggcgtg ggcgttttat
     4381 cagcgtcgcg atctgagtag cgcaaaggca gttcatgcca cggccgcgag ggaagccgag
     4441 aaccttgcgc gcttgaatct gggcgtgccc gtgcacacga tccccaacgg cgtcgacatt
     4501 ccggaattgc cggggcgcaa atccaccgga acggacgtac ctcatcgcac cgcggtcttt
     4561 ttggggcggc tgtatccggt gaaggggctg cccctcttgg tcgaagcctg gaaccgcgtt
     4621 cgccctgaag gctggcgtct ggttctggcc ggtccggacg aagccggtca ccgggccgaa
     4681 cttgaagcgc tgatcgaaaa tgccgggttg accggaacaa taactttcgc ggggccgttg
     4741 gcgggtccgg caaaatcgca gttccttgcc agcgccgagc ttttcatact tccaagttat
     4801 tcggaaagct tcggcatggc cgtagccgaa gcgctcgcgc atggcctgcc cgtcctgacg
     4861 acgagcggca cgccatggcc gatgctccat cggcgcggct gcggctggac ggtcgatgcg
     4921 accgtcgatg gcattgccga gggcttgagc gaagcgacgt cgcacgattc aaatacgctc
     4981 gcggcgatgg gcgccaaggg gcgcgcgtgg gtcgcatccg aattcggctg gcaaggcgtc
     5041 gcaatgcaat tccttgcgct ttatcgggag atatcggcgc attgatcgcg ccgtccctcg
     5101 ccgcgctcga cttggctgtc gtcctctcat atcttatcag gcgaggggtg cgcggctcgc
     5161 gtttcatcca ttcaaatcag cggggatttc ttgatcatcc ttggtcttaa tgcatttcac
     5221 ggcgactcct ccgccgcgct ggtccgcgac ggaaaactgg ttgcggccgt cgaggaggag
     5281 cgatttcggc gtgtgaaaca ttgggcggga tttccgtcgc agtccattgc ctactgcctt
     5341 cgcgaggcgg gcttaagtct ctccgacgtg gatcatgtcg cgctcaatca ggatagcagc
     5401 gcccatctct tgaaaaagat cgcttacgtc gcgctgcaca ggccgaatcc gaagctcgtg
     5461 ctgcagcgct tgggcaaccg gcgcaaacgc aaggcgatac cagagctctt ggcggacgcg
     5521 tttccggaaa cggcgtttcg cggcaaaagc cacagcatcg aacaccatct ctgccatctg
     5581 ttttcggcat tctacgtttc gcctttcaag gacgccgtgg tggtgtcggt cgacgggttc
     5641 ggcgattttt cgagcgccgc gtggggggcc ggacacggca atgacatcac cgtcgacggc
     5701 cgcgttttct ttccgcactc gctcggcatc ttttatgagg cgctcacgca atggttgggg
     5761 tttccccatt acggcgacga gtacaaggtc atggggctcg cgccctacgg caagccggcg
     5821 tatatggagc agatgcggcg catcgttcgg ctgaagccgg gcggcgcatt cgaactcgat
     5881 ttgcgctatt tcctccatca tcgcgaaatc gtgccgtatc aatgggatga cggctcgccc
     5941 gaagtcggca ccttgttctc gccggcaatg gaagagttgc tgggaccttg ccggcgcccg
     6001 ggcgcgccgc ttgagagccg ccatcacgat gtcgcccgtt ccgtgcaggc gatgtacgag
     6061 gaggctttct tccatctcat tacgaacctg cagaaacgca gcggcctgac ggatatcggc
     6121 ctcgccggcg gctgcgcggc caattcggtc gcgaacggca agatacggcg ccagacccgg
     6181 ttcgagcggg tctatgtcca gtcggcggcc ggcgacgcgg gcggcgcgat cggtgcggcg
     6241 tttgcgctgt ggcgccaact tggcggcgag cggtcttttg tcatggatca cgcctattgg
     6301 ggaacgcatt tctccaacgg cgacatcgaa gcctttatcg aaaagaaccg cagcgagatc
     6361 gatgccggcg ggtgcgcgct cgaaaacctt cccgatgaag gagaactctg ccgcaagacc
     6421 gcccaggcga tcgcggacgg caaagtcgtc ggatggtttc agggccgcat ggagtggggc
     6481 ccgcgcgcct tgggtaaccg gtcgatcgtc tgcgaccccc gccgcgccga catgaaggcg
     6541 atcctcaacg ccaagatcaa gcggcgggaa tcgtttcgcc ccttcgcgcc gtccattctg
     6601 gacgatgcgg tcggcgagtg gttcgaggaa gacgacgcgg tgccgttcat gatgcaagtt
     6661 ttccagatcc gcgaacccca gcgcgcccgg attcccgccg tcacgcacgt cgatggttcg
     6721 ggccgtctgc aaaccgtcta tcgccacacc aatccgcgat actaccggct gatcgaagcg
     6781 ttccgcgata tcaccggcgt tcccatcgtg ctcaatacgt cgttcaacga gaacgagccc
     6841 gtggtctgca atcctcagga agcgctcgac tgcttcctgc gcacgaaaat ggatgtactt
     6901 gtgctgggag acgtgatgat gacccgaggc gggttggctc gcaccgtgac ttaagccgag
     6961 tatgatttcg gccgcggcct tgttcgtgct gccctcaaga atccagcgtt cgccgcagga
     7021 acggcggcaa tttctcgacc ggtaatctat tgcggatttg gctgcgaatg gccgccccga
     7081 aatactcgag cacgagaacg attgctgcgc cgaaaagcaa gcccttgagg atggccacgg
     7141 gatagatttg gctcggccgc ggtgacagcc gcttgcgcga tgtccatggc ccgtcgaaca
     7201 catctgcagc gtagggcgcg gccgaactcg cgctcatctt gaacctctcc tgctcgctta
     7261 acgcttgcat gatggcctgg cgatgctcgg caaccgtaac ggtatccaac tccttcgaca
     7321 gatagtcgat atattgggtt gcccgcagca aagctttctt gcgtagaaga ttgtctgcgg
     7381 tctgatttac ggcgttcagc aatttgatgg caaatcgggg atcggacgag tcgcattcga
     7441 tggtggcgag atcgggccgc tttatgtctt ggtggatctc aagggtgcct ggttcattga
     7501 ggacttcctg aagccgggcg ccatcgggct gttcccacgg tagcgggcgc gcgcccaaga
     7561 gcctcatgat gccccctttg atcacctcga ccaagccagg ctggggcttt tgccacgtct
     7621 gcgatttctc gtcccattcg cgaaagaaaa tggacttcat gaggtcctta ttcttcgcaa
     7681 gttcgtctgc aagagcgcgc gaatgcatgc tgtcgaggta ggtgcggaat tgcgacgcac
     7741 tttggcctgc cgaggggctt agattcaaac cggcgagttg cgccagactg ctcagcgagt
     7801 ttgagcgcga agcagcgtga tcgcccgagc cttgaaccgg tgtaacttgc atggttacgg
     7861 caaagacgga aggggaagtg tggatattga tcaccgccag cagaaggaat accacaacgg
     7921 ctccggcgac gatcctccac ccgttaagca cgagctgcag catttcgcgc agatcgatcg
     7981 ctgacttctt ggtaacttcg ccttccgatc ctggcggcat tgaatccact ttcctgttta
     8041 ttcatcagtg cacgcgtcga cccgaagagc cgatgtctta tgtgcgtggc gtttggggac
     8101 tgctcccgtg aggcaattgg cgcaatacta ctaactaacc ggtcaattcc tagcccaatt
     8161 gcatgggaca ggaaagaggc cttgtcttgt ttatggcacg gccgttgtgt tttgaggagc
     8221 cgcccctaag cggctttgcc gatcggcgga atcatatgag atgtatcggc gtggcagccg
     8281 ttgttcttcg gcgtccgccg gccttgccgt cgaagatgcc ggtacgggcg ccctctcaaa
     8341 atagccgcct tttttcccct tgacgctcaa actgccccaa ttatcactct ttgaaatcga
     8401 tgccgtataa tcgcgagccg acgctcgcag gaacatggca agagccccgc cgtggccgca
     8461 ggtgacgttc tctgggggaa atcggcggca agtcgcaagc cctggcagga cttgccgaag
     8521 agcgaaggga aattgccgag gcgtcggtat ctggaaggcg gtcaagatcg gcgtggccat
     8581 gcgcgggtca agcgaaagag cacatgaaga atttgcttgg ctcaggataa cgaatttcaa
     8641 actgtcggct tctaaaagcg ccggctgtga cccaatgccg cgtagcaatc cgcagcgcaa
     8701 caacaggcaa agacgccggg aatatctata ctggtctgct gcgaaaaaaa gaagacgatg
     8761 aacataaacc attgttcgtt ccgggggctc catgactgaa ttcgaatcta aactttcctc
     8821 gaaaagcatc cgtgtcggcg tgatgggcct cggttatgtc ggcttgccgc ttgtgcatgc
     8881 cttttggcag gccggggtta caacggtcgg cttcgataag gaccccggca aaatcgaaaa
     8941 gctcgctcgc ggcgagagct acatcaagca tttcgcgccg gagaagatcg ccgcgatgat
     9001 tgcttcgggc cgcttcaggg cgaccgcgga tttcaacgaa ctcgccgatg ttgacgcgat
     9061 cctgatctgc gtgccgacgc cgcttgcgcc gacgcgcgac cccgatctgt cttatgtcgt
     9121 ggcgacgacg gagacgatag ccaagcatgt gcgcgccgga acgctggtgg ttcttgaatc
     9181 gaccacctat cccggcacga cgacggaagt ggtgcagcct atcctcgaaa agagcggtct
     9241 caagattggc tccgacatcc tcctggcatt ctcgccggaa cgcgaagacc cgggaaaccc
     9301 catcgagacg cgcgcgattc ccaagattgt cggcggcgtc gacgaacgct ccggcgtcgc
     9361 ggcggaaaag ttgtattcca tggcgttctt gaaagttcac aaagtgcgcg acgcgcagac
     9421 ggccgaggcg gtcaagatca ccgaaaacat cttccggtcg gtcaatatcg ccctcgtcaa
     9481 cgagttgaag gtcgtctatt cgcgcatggg catcaatgtg tgggatgtca tagaagcggc
     9541 caaaacaaag cctttcggct tcatgccgtt ttatccgggt cccgggctcg gcggccactg
     9601 cattccgatc gacccgttct acctttcctg gcaagcgcgc gcattcgacg tggacacgcg
     9661 cttcatcgaa ctggcgggcg aggtcaatcg cgccatgccg cggctggtgg tgcagacgct
     9721 cgccgaagcg ctcagcatgc gcgcatcgaa ggccctgaac ggctcggata ttctcatcat
     9781 gggcgtggcg tacaagaaga acatcgacga tcttcgcgaa agtccggctt tgcacatcat
     9841 ggacatcatc aagaaactcg gcggacgcgt ggcctatcac gatccctatc tgcccgagat
     9901 cggtccgacg cgcgagcacg cctccttgca gggcatgcga agcgtgccct tcgacgaagc
     9961 gaccgtctcc aaattcgacg ccgccctgat cacgaccgat cacgacaatg tggactacgc
    10021 caagttggtg agctggtcga agctggtcat cgacacgcgc aatgcgacga agaacgtcgg
    10081 tgcgggtaag tcgaaggtcg tgccggcctg aagaaccgtg cgtccgggtt cgggccgcgc
    10141 cttgcgggcg ccgatccgcg aggcgtgtga ggttgaattg cgctcgcttc cgccaagcgc
    10201 gaccggcctc acccaatatc cggcatcggc ggcttgccga cggcgcttca aactgttcca
    10261 actctaaagt cataaataca tggatgatct tctgttgagg cggatactga aatgatgtga
    10321 aagtattggt agttgtagcc atggatgtat tcagtaattg acgccagata cgcgattttt
    10381 attatgtttg tgttcaacga tggagagcga attattgccg gtatgccggc atgattttgt
    10441 aaatattaca acttgttccc aaaatgcgat cgggcaacgt gcggcggtcg tcggcccatg
    10501 cgccgcggca aacctaattt gcgcttgttg agcgtctttc ggggtgggtg gaatatgcct
    10561 agcctcgcgt ttacaatgtt ccgatgctac acggaccccg tgcgtggatt tgtgtgagcg
    10621 ctcgaggtat gggggagata tgactgtgat cggtcgtcct gtcggcaagc tcatttttct
    10681 aattcttgtt gcgggccttt tttctccgcg agcttacgcg caatcggcgg cgcccaatct
    10741 gcttcagcaa ctgcaacagg tgcagggcca acaaggaacc aacccgggta tcgaccaggg
    10801 catctcgcag caaacgaccg ttctggtgcc gctttcgccc acgaatccgc agctgccgac
    10861 ctcccgtctg gagcagattc tgtccgctcg cgcgggcgtc aaactcaagc agttcggcta
    10921 cgatcaatta ggggtaggcc ggccggtctc catttcgcag atgggggcgg tgcaggacga
    10981 ctatgtcctg gggcccggcg acgagatcgt ggtctcgctg cgtggacagg aaaatgccga
    11041 atatcgggtc acggtggacc gcgatgcccg ggtcgtgctg ccgcgcatga atccgatctc
    11101 cgtcggcggc cgcagttttg gagatttccg gcgggacctc gtggctgcga tccaccgcag
    11161 ctatatcgcg acggagggct ttatttcggt cgggcgggtg cgccaggtca gtgttctggt
    11221 ctccggcgag gtaaatagcc ccggcgtacg cacgcttacc ggcctttcaa cgcccgccga
    11281 tgcgattctg gtttccggcg gcatcaagaa aaccggcagc atgcgcaatg tgtatattct
    11341 gcggggcagc cgccggatcg cggtcgacct ttacagcgtc ctgacgggac atggagtagc
    11401 gagtcacgtt tcgcttggcg acggcgacag gatcgtggtg ccgcctctag gtccgactgt
    11461 agccgttgcc ggctgggtgc gcaggcccgg catctacgag atctcgggtg ggcggtcgtc
    11521 cctgaatgcg cgcgacctgc tgtcgctcgc cggcgggctt gaagtacgcg ggaagtacca
    11581 tctttccgtc ttgcgcgtgg cgccggacgg tcgcaatcgg atgtcgtccc ttgaaaacga
    11641 gtccggcgcg atcggcgaca gcgatatttt gtttgtccag ccggccgccg accagacggt
    11701 caacagcgct acgctgtccg gcggcacggc actggcgggc aagttttcca tcaccaacac
    11761 caagctttcc cagatcctga agtctcctgg cgccctcggc ggcgatccat acacgttgtt
    11821 gggaatcatc tcgcggcgcg atcccgtggc cctgttgcgg acgctgattg cgtttacgcc
    11881 ggtggccgtg ctcaagggct cgcaggacat ggacgtccag ggcgacgata tcgtgcgagt
    11941 gatctccgcc aaggaggcgc ggctgatgtt cgcgaccatt cgccgatacg aagacttgcg
    12001 gcagacctcc gaagaagcgc ttcgcagccc gtggttacag agcgtgcagc agcaggccta
    12061 cgggcaggca aacgcgacgc agcaaaatag cagctcgtcg cagcagaccc aatatggcgg
    12121 ccaacaggct ccaggcgccg cctccaccgc cggaacgccg ggggatcaga acagcaaccc
    12181 cgccgccacc cgatacttta cgccggtcct tagccccgat atggccaccc gtcaggcgct
    12241 gttgcaggcg gaagacgacc aatttagtcc ggggatggtc cagtcgcgtt tgcccaatta
    12301 tcaaagttcg tcgc
//
LOCUS       DASWPU010000471         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_83872,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000471 DASWPU010000000
VERSION     DASWPU010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83872"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..3092)
                     /gene="rpoB"
                     /locus_tag="VGC27_09995"
                     /pseudo
     CDS             complement(149..3092)
                     /gene="rpoB"
                     /locus_tag="VGC27_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729307.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
BASE COUNT          506 a         1006 c          945 g          635 t
ORIGIN      
        1 attcttcttt cctcgtcatg gccgggcggc tatcagcgcg acccggccat ccaaataccg
       61 cacggaagta cggccaatgc gtccttcaca acctgggtgg cccgttcgta cgctgtctct
      121 gcgaactcgc gggccatgac acccaggatc acccgttcac cagctcgaca ttgagaccga
      181 gcgaacgcat ttccttgacc agcacgttga agctctccgg aatgcccgct tcaaacgtgt
      241 cttcgccgcg cacgatggct tcgtagactt tggtgcggcc ggccacgtcg tccgacttca
      301 cggtcaggat ttcctgcaac gtgtaggcgg cgccgtaggc ttccagcgcc cacacttcca
      361 tctcgccgaa acgctggccg ccgaactgcg ccttgccgcc caatggctgc tgggtcacca
      421 agctgtaagg gccgatcgaa cgggcgtgaa tcttgtcgtc caccagatga tgcagcttga
      481 gcatgtagat gtagcccacc gtcaccggac ggcgaaacgc gtcgccggtg cgcccgtcga
      541 agagcgtgac ctggccggag cgcgtaagcc cggcctgatc cagcatttcg acgatgtccg
      601 cttccttggc gccgtcgaac accggggtcg ccaccggaat gccggcgcgc aggttatcgc
      661 tcagttcgag cagaccgtga tcgtcgagtt agctcaagcg ttcgtcgctg ccatagacat
      721 gcttgagcgc gcggcgaaga ccatcgaact tgccgtcgcg cttcaccttg tcgagcgcgt
      781 cgccgatctt ctggccgagg cccgcgcagg cccagcccag atgggtttcc aggatctgcc
      841 cgacattcat gcgcgaaggc acgcccagcg gattgagcac gatgtcgacc tgggtgccgt
      901 cggccagata aggcatgtcc tcgatcggca ggatgcgact gatgacgccc ttgttgccgt
      961 gacggccggc catcttgtcg cccggttgaa gcttgcgctt caccgcgacg aacaccttga
     1021 ccatcttcat cacgcccggc gccagctcgt cgccgcgccg cagcttgtcg accttgtcgg
     1081 cgaagcgctt gtcgagccgc tgcttggact cgtcgtactg ccggcgcagc gcctcgatct
     1141 cggccatcgc cttgtcgttg cgcagaccga tctgccacca ttgatgacgc gtgaccgtgt
     1201 cgagcacgct ctgcgtgatc ttggtgtccg gcgccatgcc cttggggccg aacgccgccg
     1261 tcttgttcag aagaatttcc gaaaggcggg cgaagatgtt gcgctccagg atcgccaatt
     1321 cgtcgtcgcg gtccatggcc aggcgctctt cctctgcgcg ttcgatggcc agcgcgcgct
     1381 ggtccttgtc gacgccgtgc cggttgaaga cgcgcacttc cacgatcgtg ccggtgacgc
     1441 ccggcggaac gcgcagagac gtgtcgcgca cgtcggccgc tttttcgccg aagatggcgc
     1501 gcagcagttt ttcttccggc gtcatcgggg tttcgccctt gggcgtgacc ttgcccacca
     1561 agatgtcgcc cgcgttcact tccgcgccga tatagacgat accggcctcg tccaggttct
     1621 tgaggttctc ttcgccgaca ttgggaatgt cgcgggtgat ttcctccggc ccgagcttgg
     1681 tatcccgcgc catggtctcg aattcctcga tgtggatcga ggtgaagacg tcatcgcgca
     1741 cgatgcgttc ggagatgagg atggaatcct cgaagttgta gccgttccac ggcatgaagg
     1801 cgaccagcgc gtttttgccc agcgccagct ctccgagctg cgtggacggg ccgtcggcga
     1861 tgatgtcgcc tttttgcagc acatcgccga ccttcaccaa cggcgtttgc gtgatgcagg
     1921 tcgattggtt ggaacgctgg aacttcttca agcgatagat gtcgatgccc ggcttggtcg
     1981 gatcgggttc ttcggtggcg cggatgacga tacgcgtcgc gtccacctgg tccaccacgc
     2041 ccgcccggcg cgccgcaatg gcggaaccgg agtcgcgcgc aaccacgcct tccaatcccg
     2101 tgccgaccag cggcgcttcc ggccgcaaca gcggcacggc ctggcgctgc atgttcgagc
     2161 ccatcagggc gcggttggcg tcgtcgtttt ccaggaacgg gaccagtgcg gcggccaccg
     2221 aaaccagctg cttcggcgac acgtcgatga attccacctg gtcgggcgtg gtcatcacat
     2281 agtttgcgcc cttgcggcag gacaccaatt cgtcggtgat ccgcccgcgg ttgtccaccg
     2341 aagcattggc ctgggcgatg gtgtacttcg cctcttccat cgcggacaga tagaccacct
     2401 cgtcggtgac gtgcttgttg tgcacgcgcc ggtacgggct ttcgatgaag ccgtacttat
     2461 tgacccgcgc ataggtcgcc agcgaattga tcaggccgat gttcgggcct tccggcgttt
     2521 cgatcgggca gatgcgacca taatgcgtcg gatgcacgtc gcgtacttcg aagccggcgc
     2581 gttcgcgcgt cagaccaccc ggtccgagcg ccgacatgcg ccgcttgtgg gtcacttccg
     2641 acagcgggtt ggtctggtcc atgaattgcg aaagttgtga ggacccgaag aactcgcgca
     2701 ccgccgccgc cacgggcttg gcgttaatca ggtcatgcgg catcacggta tcgatatcga
     2761 ccgcgctcat ccgctccttg atcgcccgct ccatgcgcaa caggccgatg cggaactggt
     2821 tttccatcag ttcgccgacc gagcgcacgc gccggttgcc gagattgtcg atatcgtcga
     2881 tctcgccgcg gccatcgcgc agttcggcca gcgccttgac gatcgccaag atgtcttcct
     2941 tgcgcagcgt gcgcatcgta tcgggggcat cgaggttaag ccgcatgttc atcttcaccc
     3001 ggcccacgct cgaaagatcg tagcgctcgg aatcgaagaa caactggttg aacagggttt
     3061 ccgccgtgtc gagcgtcggc ggttcgcccg gg
//
LOCUS       DASWPU010000472         3321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_65779,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000472 DASWPU010000000
VERSION     DASWPU010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65779"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VGC27_10000"
     CDS             complement(<1..763)
                     /locus_tag="VGC27_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0282941.1"
                     /translation="MYNKSIQIKFAGVDKDFELGGAKHKVLVSIDLEVPRGEVFGIIG
                     ASGAGKSTLIRMINRLVRPSRGRVEVDGVDLARLSPRELSARRQKIGMIFQQFGLLSS
                     KTARQNVLYALELARAGTREARRAKVDTLLERVGLAHHADKYPAQLSGGQKQRVCIAR
                     ALANDPEVLLCDEATSALDPESTADVLALLNELNRTLGLTIILVTHEMDVVRRACDRV
                     AILDKGRLVEVGEVAQVLFEPRTETARALGRHLLPQ"
     gene            complement(987..2255)
                     /locus_tag="VGC27_10005"
     CDS             complement(987..2255)
                     /locus_tag="VGC27_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEY0282942.1"
                     /translation="MTDPDPLGALIEAWRSDAGGTYRTWFLWEERVKNFRSIRRGLQQ
                     VVSLISDGTFGNAYRGSALETVVGSIAEQRQIFRGADHPFLWKPKLRIPDIYESVENQ
                     KAFGALLDTCLCCDGERQIIEAIHAIDAKAIKGLGPAVANLLYFLHPTFVPPFNTAIV
                     KGYNAITGSKVKLGRWQEFLAMREGMLRLNGAYRQLLSNDLGAIGGFLFDVGNGRYVP
                     PPLNDEPAAMAAWRAELDRARAEAANANAALVKAASDDHTHTKVQGWLRDLGLALGFH
                     VWIAANDQNRNYNGDKLGKGCIDDLPVRGAGMGAVRLIDVIWLEKTSGAFVAAFEVEH
                     TTSIYSGIVRLLDLALGEECRSLQGLFLVAPDDREQEVREQLRRPAFSRVADLKVRYL
                     PYGELDANRNAIVRFGQGMKPIEAISRLLG"
     gene            complement(2368..3165)
                     /locus_tag="VGC27_10010"
                     /pseudo
     CDS             complement(2368..3165)
                     /locus_tag="VGC27_10010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN.; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
     gene            3094..>3321
                     /locus_tag="VGC27_10015"
     CDS             3094..>3321
                     /locus_tag="VGC27_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282943.1"
                     /translation="MIEDVQLGRIEEAIVRFVEQERLVVPTVPEALDDLNIFGGAGVP
                     LLMADLFLEAEIARFGIRGGGDEVPTGPAAAD"
BASE COUNT          653 a         1061 c          966 g          641 t
ORIGIN      
        1 gttgcggaag caaatgccgg cccagcgccc gtgccgtttc cgtccgcggc tcaaacagca
       61 cttgtgcgac ctctcccacc tccaccaggc gccccttgtc gagaatggcg acgcggtcgc
      121 aggcacgccg caccacgtcc atctcgtgcg tcacgagaat aatggtgagc ccgagcgtgc
      181 ggttgagttc gttcagcagt gcgagcacgt cagccgtcga ttccggatcg agcgcactgg
      241 tggcttcgtc gcacagcagc acttccggat catttgccaa ggcgcgggca atacagacgc
      301 gctgcttctg cccgcccgaa agctgcgccg ggtatttgtc agcgtggtgc gccagcccca
      361 cgcgctctag caacgtatcc accttggctc ggcgcgcttc ccgcgttccg gcacgcgcaa
      421 gctccaaggc gtaaagcacg ttctgccggg cggtcttgga cgacagcaat ccgaactgct
      481 ggaagatcat ccctatcttc tggcggcgcg cggaaagctc gcgcggcgac agccgcgcca
      541 gatccacgcc atcgacctcc acccggccgc ggcttggccg caccaggcgg ttgatcatcc
      601 ggatgagcgt ggattttccg gcgcccgagg caccgatgat gccaaacact tcgccgcgcg
      661 gaacctccag atcgatggag accagcactt tgtgcttcgc cccgcccaac tcgaagtcct
      721 tatcaacacc cgcgaatttg atctggatag acttattata catattcttt actgttgtat
      781 gtgaacgaat tgaacacatt tccaatatat tataccttct gatcaaaata acagatatag
      841 gcagtgatat gagacaaagt acggcaagag ttgtggactt ggcgcccgtt caccttcccg
      901 ccgcgctaag ccaatggccc aacggccagg tacatgatcc acatgttcga tatgcgctgt
      961 agcgagaacg gcatcgaatt cggcattcag ccgagtagcc gcgaaattgc ctcgattggc
     1021 ttcatgccct gcccgaagcg gacgatcgca ttgcgattgg catcgagctc gccgtagggg
     1081 agatagcgca cctttaaatc ggcaactctg ctgaacgccg gccgccgcaa ttgctcacgc
     1141 acttcttgtt cgcggtcgtc gggagcaacg aggaaaagcc cctgaagcga cctgcactcc
     1201 tctcccagcg caaggtccag aagacgcacg attccggaat agattgaagt cgtgtgttct
     1261 acttcgaagg cggccacaaa ggcgccagag gtcttttcca gccatatgac atcgataaga
     1321 cgcaccgcac ccataccggc gccgcgcacg ggaagatcgt cgatgcatcc tttgccgagc
     1381 ttatcgccgt tgtaattccg gttttggtca ttcgcagcaa tccagacgtg aaagccgagc
     1441 gccagtccga gatcacgcag ccaaccttgt accttcgtgt gcgtatgatc gtcgctggcc
     1501 gctttcacca gagccgcgtt cgcgttcgcg gcttccgccc gtgccctgtc gagttcggcg
     1561 cgccacgccg ccattgccgc gggttcgtcg ttcagcggcg gcggcacata gcggccgttt
     1621 cccacatcga acaggaaacc gccgatggct cccagatcgt tggagagcaa ctgccggtac
     1681 gcgccgttca ggcgcaacat tccttcgcgc atggcgagga actcctgcca acggccgagc
     1741 ttcaccttgc tgcctgtaat cgcgttgtag cccttcacaa tggcggtgtt gaacggcggc
     1801 acgaaagtgg ggtgcagaaa atacagcaga ttggcaacgg cgggaccgag ccccttgatc
     1861 gccttcgcat ctattgcgtg gatggcttct atgatctgcc gctccccatc acagcacagg
     1921 caggtgtcga ggagagcgcc aaatgccttc tggttttcca cgctctcgta aatatcgggg
     1981 atacgcagct tcggcttcca gaggaacgga tggtcggcgc ccctgaatat ttgccgctgt
     2041 tcggcaatcg atccgaccac ggtttccaat gcggagccgc gatatgcatt tccgaaggtg
     2101 ccgtccgaga tgagggaaac gacctgttga aggccccgcc ggatggagcg gaaattcttg
     2161 acccgctctt cccacaggaa ccaggtccgg tacgtgccgc ccgcgtccga gcgccacgcc
     2221 tcgatcaacg caccgagcgg atcagggtct gtcatcggcg gcagtatagc gcataaagcg
     2281 ccttgacgac atgcagcgct ttggcatcgg cgatgcggta gtagatcgtt tgaccatcgc
     2341 gccgcgcgcg cccaagcgca gtgggaccta tcgcctctcc ggtttcggca agccgagatg
     2401 cccacgcagg gtcgtggctt catactccgt gcggaagcgg ccgcggcgtt gcagttcggg
     2461 cacgacaagc ccggcgaaat cctgaagccc ttcaggcaga ataggcgcca tcacgttgaa
     2521 gccgtcggcc gcaccaccgt cgacccagct ttccatctga tcggcgattt cgccggccgt
     2581 tccgattgcc gtccaatgtc cgcgtccgcc cgcaactttc agcatcaact ggcggagcgt
     2641 gagaccgcga gcgatctcgc tgaacagctt gatccggctt tgcatgcctt cgttctgcgc
     2701 aagttccgtc acgggctgat ccaactggtc cggcgagaag aggattcccg tcatcgcgta
     2761 caacaaaccc aaactgtgcg ctggatcgat caaggactga agttgatcga actttgcctg
     2821 cgcctccgcg cgcgtgcgcc cgacaaatgc cagcaggccc ggcataatgc gaacctcgtc
     2881 cggcgaacgg cccgcatcgc caagccgcgc acggagtcca tcggcgaagg ccttggcgcc
     2941 ggataggccg ctctgggccg tgaacacgac gtccgccgtc gcggcggcca atgcctggcc
     3001 gtcgtccgac gaacccgcct gaacgatgag cgggcggcct tgcgggctgg ggggcaacga
     3061 caagggtccg cgcaccgaat agtacgcgcc ttcgtgatcg aggacgtgca gcttggccgg
     3121 atcgaagaag cgattgtgcg ctttgtcgaa caggaaaggc tcgtcgtccc aactgttcca
     3181 gaggcccttg acgacctcaa catattcggc ggcgcgggcg taccgctact tatggccgat
     3241 ctgttccttg aggccgaaat tgcgcgcttc ggcatccgtg gcggaggtga cgaggttcca
     3301 accggcccgg ccgccgctga t
//
LOCUS       DASWPU010000473         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_114600,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000473 DASWPU010000000
VERSION     DASWPU010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114600"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            531..1928
                     /locus_tag="VGC27_10020"
     CDS             531..1928
                     /locus_tag="VGC27_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0282944.1"
                     /translation="MTKSFPSAEDRSDRILVEKLRLLYRGSFAVPFNLAIACVVAFLL
                     WDSFPRPVLVVWLVATALVAGLRLLLHRKFLQAVARGMCSVCWAARFCIGAFASGLIW
                     GVLALGLPVWGGPNEYVLLTLIGAGMSAGALTAIVAYLPAYLVYATSFVLPLVAALLL
                     HPDQHIAATGWLMLLYLLVIGFAARNLSRSVNRTIELQVDNEVLHNSLVQVRIERDDA
                     QTEKWSTLAQLSHELRTPLNAILGFSEAMREEIFGPLGDKRYKDYVAHVHNSGSHLLT
                     LILEILQLSQGEAGKMVLNEGTVDLAAAVAKCVEDLMPAARKAELTLKSFVPPDLPLI
                     RADDAKTRQMLSYLADNAIKFTPPHGAVLIEAAQAADGGIDLIVRDTGIGMKAEDIPL
                     ALQPFGRIATPLKQATGGMGLGLSICMRMAELHGAELTLNSEPGKGTECRISFPASRR
                     IGPPAAQAGKSAVAA"
     gene            2105..>2327
                     /locus_tag="VGC27_10025"
     CDS             2105..>2327
                     /locus_tag="VGC27_10025"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEY0282945.1"
                     /translation="MTAHQSDRFVPPLPLREGPRAKGARGRDSYIARARELRKEATEA
                     EKMLWERLQRKQMGGLRFRRQYPLGPYFAD"
BASE COUNT          428 a          727 c          711 g          461 t
ORIGIN      
        1 ggcctggtcg gcaagggcca tatcaatctc gccgagacga tggccgagcg catcgccgcc
       61 gtctgtttcg aggataaacg cgtgaagtcc gcgcgcgtac gggtggagaa actgcacgcc
      121 ctgcccggcg caggctccgc gggtgtcgag atcgagcgta accggcccgc tcgctagagc
      181 aaatcaccgt ttattggaat cggtgatttg ctctagctat ttgttttgtc gcgcttctta
      241 tccgaaaacc gccgaatacg cgtcattcgc tgccgctcat gacctatcgg ccccactttt
      301 cgggaagcgc tctaggacca tcgcgtccca aggccgaggc tcgatttgtt ccttcctcat
      361 gggaatgaaa aagcccgcgg cgccagcctc ttctgccccg atcgaaccgg gatatgcccc
      421 cagcccgggc aatccatgtc atttacggtg cattaaacct gaaatgcgta ccctaggtag
      481 caaactcccc gtgcgaattg tgcccacggg cgctcacgca tgagtaacgg atgaccaagt
      541 cattcccgag cgccgaggat cgctcagacc ggattctggt cgagaagctg cggctgcttt
      601 atcgtgggag tttcgcggtt ccgttcaatc tcgcgatcgc gtgcgtcgtc gcgttcctcc
      661 tgtgggattc gtttccccgc ccggttcttg tcgtctggct tgttgcgacc gcgctggtgg
      721 cgggcctgcg cctcctgctt cacaggaaat tcctgcaggc cgtcgcgaga ggcatgtgca
      781 gcgtgtgttg ggcggcgcgc ttttgcatcg gtgcgttcgc gtcggggctg atttggggcg
      841 tgctcgctct cggcctcccc gtctggggcg gccccaacga gtatgtcctc ttgacattga
      901 taggcgcagg gatgagcgcc ggggccttga cggcgatcgt agcctatctg ccggcctatc
      961 tcgtttatgc cacgtcgttc gtcttgccgc ttgtcgccgc cttgctgctg caccctgacc
     1021 aacatatcgc ggcgacgggc tggcttatgc ttctgtacct gctggtcatc ggcttcgccg
     1081 ccaggaatct gtcccgctcg gtgaaccgga ccatcgaact tcaagtcgac aacgaggttc
     1141 tgcacaattc gctggtgcag gtccgcatcg aacgcgacga cgcccagacg gagaaatggt
     1201 ccacgctcgc gcaattgagc cacgaactgc ggacgccgct gaacgccatc ctgggcttct
     1261 cggaagccat gcgcgaggaa atcttcgggc ctctcggcga caaacgatac aaggactatg
     1321 tcgcacatgt gcataacagc ggcagccatc tgctgacgct tatcctcgaa atcctgcaac
     1381 tgtcccaggg cgaagccggc aagatggtgt tgaacgaagg caccgtggac ctggcggcgg
     1441 ccgtcgcaaa atgtgtcgaa gacttgatgc ccgccgcccg caaggcggag ctgacgctga
     1501 agagtttcgt gccgccggat ttgccgctca tccgcgccga cgacgccaag acccgccaga
     1561 tgctgtccta tctcgccgac aacgcgatca agttcacccc gccgcacgga gcggtcttga
     1621 tcgaggccgc gcaagcggcc gacggcggta tcgatcttat cgtccgggat acgggaatcg
     1681 gcatgaaggc ggaggacatc ccgctcgcgc tgcagccctt cggccggatc gcgaccccct
     1741 tgaaacaagc aaccggggga atgggcctcg gcctttccat ctgcatgcgg atggccgaat
     1801 tgcacggcgc cgagctgacc ctcaacagcg aaccgggcaa gggcacggaa tgcaggattt
     1861 cgtttcccgc ctcgcgccgc atcggtccgc ccgccgcaca agccggcaaa tccgccgtcg
     1921 cggcgtgaag gacgccatgg tacccggtgc cgtgtccgct tgtgttgaaa tcaaacggcg
     1981 gcggccatgc gcgatcgagt aagtgctcat caactcgctg cgatggttct tcacccggaa
     2041 cagtcaggag cttaaaacgg cgatcgaccc tctgcacgct gcagacgggc gacggagaga
     2101 gcgcatgacc gctcatcaaa gcgaccgttt tgttccgccc ctccccctgc gggaggggcc
     2161 gcgcgccaaa ggtgcgcggg ggagggattc ctatatcgcc cgagcgcgtg aattgcgaaa
     2221 ggaagcgaca gaggcggaaa agatgctctg ggagcgtctg caacgaaagc agatgggagg
     2281 tttacgcttt cgacgacagt atcccttggg gccgtatttc gcggatt
//
LOCUS       DASWPU010000474         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_83898,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000474 DASWPU010000000
VERSION     DASWPU010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83898"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..996
                     /locus_tag="VGC27_10030"
     CDS             337..996
                     /locus_tag="VGC27_10030"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0282946.1"
                     /translation="MELPDAAKQRKDDRRAAILEIARTAFLQDGYSGTSMSQIAVRLG
                     GSKATLYNYFSSKKELFVALADEEATRIFLPLFDVSEMRGGVREVLVNFARRFLALLL
                     SDDLIAFYRLVIAESARFPEVGLTTYEIAIKRGLERMVGYFNEATERGELRRANAVVA
                     AEQFLDLCAGHLHRKRLWGVIGDVGPKEIEAQAERSVSLFLAAYGNDELSLAARQFTG
                     L"
     gene            1059..2012
                     /gene="fabD"
                     /locus_tag="VGC27_10035"
     CDS             1059..2012
                     /gene="fabD"
                     /locus_tag="VGC27_10035"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY0282947.1"
                     /translation="MRAFIFPGQGSQAVGMGKVLAEAFAPAREVFQEVDEALSQPLSR
                     LIWEGPESDLILTANAQPAIMAASMAVIRVLQREAGLDLGRHARLVAGHSLGEYTALC
                     AAGSFTLGDTARLLKIRGRAMQEAVPVGIGAMIALIGADVALAEDAAKEAVAKATEAD
                     PVCVVANDNAPGQVVISGRKATVDRAGEIAKAKGVKRALPLAVSAPFHCPLMKPAADK
                     MAEALAAVTIRPPAATLIANVTAAETNEPETIRRLLVEQVTARVRWRESVLAFRQAGV
                     ELTVEAGGNKVLTGMVKRIDKELANTTLDTPADIEAFARTL"
     gene            2012..2752
                     /gene="fabG"
                     /locus_tag="VGC27_10040"
     CDS             2012..2752
                     /gene="fabG"
                     /locus_tag="VGC27_10040"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY0282948.1"
                     /translation="MFDLTGKTALVTGASGGIGGAIAKGLYAQGASVVLSGTRAEVLE
                     GLKSELGSRAYTAVCDLTDAASVEALPKAAEAVAGAVDILVNNAGITSDNLFMRMKDE
                     EWDRVIAVNLTAAFRLSRAVLRGMMKKRWGRIVSITSVVGAIGNPGQGNYAAAKAGLT
                     GMTKSLAAEIASRNITVNCVAPGVVATAMTDVLSEDQMKAWLGKIPAGRFGSPDDVAA
                     AVVYLASNEAGYITGQTLHLNGGMAMIS"
BASE COUNT          585 a          857 c          978 g          531 t
ORIGIN      
        1 cgcttgtagg ggcggcaaag ctcctggcgg tttcatagct cccggccggc ttgatctggg
       61 gcttgtcgcc caggttcgga gcgcaggcgg cgagcgtaaa aaaagcaagg gcggcggcac
      121 agactggggg taggccgcgc cgggccttat ggccgctgcc gcccttgcaa ctcgtcaagt
      181 aagggtaagt gattttgtaa cacattgaac catatccgaa atatactatg accgtaccgt
      241 acagtactat tgtgttggca tcaatttggg gatagcttcg ggaaaagtca acgctaaact
      301 gtacggtacg gtcacaagaa aatggaaacc cgaacaatgg aattgccaga tgccgccaag
      361 cagcgaaaag acgatcgccg ggcggcgatc ctcgagatcg cgcgcaccgc cttcctgcag
      421 gatggctatt ccggaacgtc gatgtcgcag attgcagtgc gcttgggcgg gtcgaaagcg
      481 actctctaca attatttctc ttccaagaaa gagctgttcg tcgcgctggc cgacgaagag
      541 gcaacgcgca tattcctccc cctgttcgac gtcagcgaga tgcgcggagg cgtccgcgag
      601 gtgctggtga atttcgcccg gcgcttcctg gctttgctgc tgtccgacga tctgatcgcc
      661 ttctatcggc tggttatcgc ggaatcggcg cggtttcccg aagtcggcct aaccacctat
      721 gaaatcgcca tcaaacgcgg ccttgagcgc atggtgggat atttcaacga ggcgacggaa
      781 agaggcgagc tgcgccgggc gaatgctgtc gttgcagcgg aacaattttt ggatctttgc
      841 gccggacatc tgcacagaaa gcgattgtgg ggcgttatcg gcgacgtcgg cccgaaggaa
      901 atcgaggccc aggccgagcg cagcgtgtcg ctctttctag ccgcttatgg caacgacgag
      961 ttgagcctgg ccgcgcgcca atttaccggc ttatgaccgg cgcttgactt cgcgttcgtc
     1021 gcttgatttg tctgccgccc gatttggggg ccagagcgat gcgcgcattc atctttccgg
     1081 gacaaggcag tcaggccgtc ggcatgggca aggtgctggc ggaagccttc gcgccggccc
     1141 gcgaggtgtt ccaggaagtc gatgaagcat tgtcgcagcc tctctcccgc ttgatatggg
     1201 aaggaccgga aagcgacctg attctcaccg cgaacgcgca accggcgatc atggcggcca
     1261 gcatggccgt catccgcgtc cttcagcgcg aggccggatt ggatttgggg cgtcacgcac
     1321 gcctcgtcgc cggacattcg ctgggcgaat acacggcgct gtgcgcggcg ggcagtttca
     1381 cgctcggcga taccgcaagg ctgttgaaaa ttcgcggccg cgccatgcag gaagcggttc
     1441 cggtcgggat cggcgccatg atcgcgctga tcggcgccga tgtcgcgctt gccgaagacg
     1501 cggcgaagga agcggtggct aaggccacgg aagccgatcc ggtctgcgtc gtcgccaacg
     1561 acaacgcgcc gggacaggtt gtgatctccg ggcgcaaggc gacggtggat cgcgcaggcg
     1621 agatcgcgaa ggcgaaaggc gtcaagcgcg cgctgccgct cgcggtcagt gcgccgttcc
     1681 attgcccgct gatgaagccc gccgccgaca agatggccga ggcgctggcc gccgtgacga
     1741 ttcgcccgcc cgcggcgacg ctgatcgcca acgtcacggc cgccgaaacc aacgaacctg
     1801 agacgatccg tcgtcttctg gtggagcaag tcacggcgcg cgtgcgttgg cgcgaaagcg
     1861 tgctagcctt ccgccaggct ggcgtggaac tgacggtcga ggccggcggc aacaaggtgc
     1921 tcacgggcat ggtcaaacgc atcgacaagg aattggccaa cacgacgctc gatacgccgg
     1981 ccgacatcga agccttcgcg aggacgttgt gatgtttgat ctcacgggta agaccgccct
     2041 ggtgacgggc gcttccggcg gcatcggcgg cgccatcgcc aaggggctgt acgcacaggg
     2101 tgcgagcgtg gtgctttcag ggacgcgcgc cgaggtactt gaaggcttga agagcgaact
     2161 gggtagccgc gcctatacag ccgtctgcga tcttaccgat gcggcttcgg tggaagcctt
     2221 gcccaaggcg gctgaagccg tcgccggcgc ggtcgacatt ctggtcaaca atgccggcat
     2281 taccagcgac aatctcttca tgcgcatgaa ggacgaggag tgggaccggg tgatcgccgt
     2341 caacctgacg gcggcgttcc gcctgtcgcg cgcggtgctg cggggcatga tgaagaagcg
     2401 ctggggacgc atcgtgtcga tcacctccgt cgtcggcgcc atcggcaatc cgggccaggg
     2461 caattacgcg gcggcgaagg ccggtctcac cgggatgacg aaatcgcttg cggccgagat
     2521 cgccagccgc aacatcacgg tcaactgcgt ggcgccgggc gtcgtagcaa ccgccatgac
     2581 cgatgtcctg agcgaggatc aaatgaaagc ctggctcgga aagatcccgg ccgggcggtt
     2641 cggttcgccg gacgacgttg ccgcggccgt ggtctatctc gccagcaacg aagcgggcta
     2701 catcaccggc cagactctgc atctcaatgg cggcatggcg atgatttcat agaaaatcca
     2761 atggcttgcg ctctggtggc ggtgcggata ggtttatgtt acgaaaccgg gaccccacta
     2821 ggcgttggca taggtgaggg ccctcgccgc ggcccgcaac gaacataaac gcggtggaaa
     2881 aagcaaggag aggcaaaaaa agccatgagc gatactgccg aacgcgtgaa gaagatcgtc
     2941 gtcgagcatc t
//
LOCUS       DASWPU010000475         3777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_47068,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000475 DASWPU010000000
VERSION     DASWPU010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47068"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VGC27_10045"
     CDS             <1..314
                     /locus_tag="VGC27_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0282949.1"
                     /translation="RQWQKLLHGGRYSHSYSETLPDFVRLAEAFGCVGLRAETPDELD
                     AKIMAMIETPKPVIFDCRVDQMENCYPMIPTGAAHNEMILCDAEEESAPAGDAEEELS
                     V"
     gene            314..586
                     /locus_tag="VGC27_10050"
     CDS             314..586
                     /locus_tag="VGC27_10050"
                     /inference="COORDINATES: protein motif:HMM:NF045463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282950.1"
                     /translation="MRYLYVKWLHKNPNDPVHLYSEVGEDSYERRKVEIYADGRRGFA
                     DASEEFGGTVLGSMPVPSLKDIAALPEFEPKEIAQEEFQRIWLKRR"
     gene            583..1092
                     /gene="ilvN"
                     /locus_tag="VGC27_10055"
     CDS             583..1092
                     /gene="ilvN"
                     /locus_tag="VGC27_10055"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004697653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY0282951.1"
                     /translation="MTPPKIERHTIAVLVDNEAGVLARVVGLFSGRGYNIESLTVAEV
                     DPNAHTSRITIVTSGSVEVLEQIRAQLGRLVPVQNVVDWTIDKPGIEREMALIKVAGT
                     GEKRVEALRMAGAFRARPVDTTHTSFVFEITGAPSKLDTFIDLMRPLGLVEVSRTGVA
                     AISRGPGAM"
     gene            1243..2259
                     /gene="ilvC"
                     /locus_tag="VGC27_10060"
     CDS             1243..2259
                     /gene="ilvC"
                     /locus_tag="VGC27_10060"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004672686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY0282952.1"
                     /translation="MRVYYDRDADLNFIKNKKVAVIGFGSQGHAHALNMRDSGVKEVA
                     IAVRPGASAKKAEAAGFKVMTSAEAAKWADAMMMATPDELQADIYKNDLAPNMKEGAA
                     LFFAHGFNVHFRLIEPRADLDVLMVAPKGPGHTVRSEYQKGGGVPCLIAVQQDASGNA
                     HDLGLAYASAIGGGRAGIIETTFREECETDLFGEQNVLCGGLVELIRAGFETLVEAGY
                     APEMAYFECCHEVKLIVDLIYEGGIANMNYSVSNTAEYGEYVSGPRIVTAETKAEMKR
                     VLDDIQSGRFARDWVLENKAGQPSFKAMRARAAAHPIEEVGAKLRAMMPWIAKNKLVD
                     KDKN"
     gene            2423..>3777
                     /locus_tag="VGC27_10065"
     CDS             2423..>3777
                     /locus_tag="VGC27_10065"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0282953.1"
                     /translation="MRRILVFAAVAFLAVPACAADPRTTAALRDKALTDSTAWDVLES
                     LTTEIGPRPVGSAAALRARDWALAKLKALGFTNVHAEPFAKPSWARGAESAEVLTPYP
                     QKLAVIGLGGSVPTPPQGIEAPIVVLKTYADLLAAPLGAFKNKIVVVNQPMTRAQDGS
                     GYGKAVAARYGATEAAKRGAVAYLVRSISTGNARAPHTGSGTYGPKASRIPEAALGVP
                     DADLLERMAARGPVRLRLKLASFLRPRSVAWNVSGEIAGSARPDQVIVVGGHLDSWDS
                     GTGAVDDGAGVAITTAAARLIGQLPRHPRRTIRVVMWGSEETGGSSGAYLAAHKGELA
                     KIVVASESDLGSDRVYRLKLPQGALARPQLKPLAGILAPLKVAVSPEPVQHAGSDVEG
                     LQKAGVPVFSLDQDASRYFDFHHSADDTLAIVDRAQLNQNVAAWAVLLYLIADSDVDF
                     RA"
BASE COUNT          678 a         1179 c         1278 g          642 t
ORIGIN      
        1 tgcgccagtg gcagaaattg ctgcacggcg ggcgctattc gcattcctat tcggagacgc
       61 tgcccgattt cgtcaggctg gcggaagcgt tcggctgcgt cggcctgcgc gccgagacgc
      121 cggacgaact cgacgccaag atcatggcga tgatcgagac gccaaagccc gtgatcttcg
      181 attgccgcgt cgatcagatg gagaattgct acccgatgat tccgacgggc gcggcccaca
      241 acgagatgat cctctgcgac gcggaggaag aatcggcgcc ggccggcgac gcggaagaag
      301 agctgtccgt ctgatgcgat atctatacgt caaatggttg cacaagaacc cgaacgatcc
      361 ggttcacctc tacagcgaag tgggcgagga ctcctatgag cgccgcaagg tggaaatcta
      421 tgcggacggc cgccgcggct tcgccgacgc cagcgaggaa ttcggcggga cggtcttggg
      481 ctcgatgccg gtgccgtcgt tgaaggacat cgcggctctg ccggaattcg agccgaaaga
      541 gattgcacag gaagagttcc aaaggatttg gctgaaacgc agatgacacc tcccaagatc
      601 gaacgtcaca ccatcgccgt gctcgtggac aacgaggcgg gcgtgctcgc gcgggtcgtg
      661 ggcctgttct ccggccgcgg ctacaacatc gaaagcctga ccgtggccga ggtcgatccc
      721 aacgcgcaca cctcgcgcat caccatcgtt acctccggct cggtcgaggt gctggagcag
      781 atccgcgccc agctcggccg cttggtgccg gtccagaacg tggtggactg gaccatcgac
      841 aagcccggca tcgagcgcga aatggcgctg atcaaggtcg ccggcaccgg cgagaagcgg
      901 gtggaggcgc tgcgcatggc cggcgccttc cgggcgcggc ccgtcgacac cacgcatacc
      961 agcttcgtct tcgagatcac cggggcgcct tccaagctcg acaccttcat cgacctgatg
     1021 cgtccgctcg gtctggtgga ggtctcgcgc accggcgtcg ccgccatctc gcggggaccg
     1081 ggggcgatgt gacaataaaa ccttgtcatg gccgggcagc gagcgtagcg aagcgcgacc
     1141 cggccaccca gccgccgcgc gtccgcgcgg caattgaatc ttatgcccac ctgggtggcc
     1201 cgcattcgcg ggccatgaca tctatcgctt aggagaaatg gaatgcgcgt ctattacgat
     1261 cgggatgccg atctgaactt catcaagaac aagaaagtgg ccgtcatcgg cttcggctcg
     1321 cagggacacg cgcatgcgct gaacatgcgc gatagcggcg tgaaggaggt cgcgatcgcg
     1381 gtgcgccccg gcgccagcgc caagaaggcg gaggccgcgg gcttcaaggt gatgacgagc
     1441 gccgaggccg ccaaatgggc cgacgcgatg atgatggcga cgcccgacga gcttcaggcc
     1501 gacatctaca agaacgatct cgctcccaac atgaaagagg gcgcggcgct gttcttcgcc
     1561 cacggcttca acgtgcattt ccgcctgatc gagccgcgcg ccgatctcga cgtgctgatg
     1621 gtcgcgccca aggggccggg ccatacggtg cgcagcgaat atcagaaggg cggcggggtg
     1681 ccctgcctga tcgccgtgca gcaggacgcc agcggcaacg cccacgacct cggcctcgct
     1741 tacgcctccg ccatcggcgg cggccgcgcc ggcatcatcg aaacgacttt ccgcgaggaa
     1801 tgcgagaccg acctgttcgg cgagcagaac gtgctctgcg gcgggctggt ggaactgatc
     1861 cgcgccggct tcgaaacgct ggtggaagcg ggctacgcgc cggagatggc ctatttcgaa
     1921 tgttgccacg aggtgaaact gatcgtcgat ctcatctacg agggcggcat cgcgaatatg
     1981 aattattccg tctccaacac ggcggagtac ggcgaatatg tcagcggtcc gcgcatcgtc
     2041 acggccgaga ccaaggccga gatgaagcgg gtgctcgacg acatccagtc cggccgcttc
     2101 gcgcgcgact gggtgctgga gaacaaggcc ggccagcctt cgttcaaggc gatgcgggca
     2161 cgtgccgccg cccatccgat cgaggaagtc ggcgccaagc tgcgcgccat gatgccctgg
     2221 atcgccaaga acaagctggt agacaaggat aagaactagc ggttcaggaa tatctcgtct
     2281 cgcccggcga agcggcgaaa gctgcgaagc cgggtggatc agacgaagaa ttgacggacg
     2341 cacttgccgc cttcatgctt cggcgtgctc agcatgaggg atcaccttca ccctgagctt
     2401 gtcgaagggt gagggggtgt ccatgcgtcg catactggtt tttgcagccg ttgcatttct
     2461 tgccgtgccc gcttgtgcgg ccgatccgcg cactaccgcc gccttgcgcg acaaagcgct
     2521 gacggattcg accgcttggg atgttctgga atcgctgacc accgagatcg gtccgcgtcc
     2581 ggttggcagt gcggcggcgc tgcgggcgcg cgattgggcg ctggccaaat taaaggcgct
     2641 cggctttacc aacgtccatg ccgagccgtt cgccaaaccg tcctgggcgc gcggtgccga
     2701 gtcggccgaa gtgctcacgc cttatccgca gaagctggcc gtcatcgggc tgggcggctc
     2761 ggttcccacg ccgccgcagg gcatcgaagc gccgatcgta gtgctgaaaa cctatgccga
     2821 tttgcttgcc gcgccgcttg gcgctttcaa gaacaagatc gtggtggtca accagccgat
     2881 gacgcgcgcc caggacggtt cgggttacgg caaggcggtg gcggcccgct acggcgctac
     2941 ggaagccgcc aagcgcggcg cggtggccta tctggtgcgc tcgatctcca ccggcaacgc
     3001 gcgcgcgccc cataccggca gcggcactta tgggccgaag gcgtccagga ttcccgaggc
     3061 ggccctcggt gtgccggatg cggacctgct ggagcgcatg gcggcgcgcg ggccggtgcg
     3121 cctgcgcctg aagcttgctt ccttcctgcg tccgcgttcc gtggcgtgga atgtgtcggg
     3181 ggagatcgcc ggcagcgcac ggccggacca ggtgatcgtc gtcggcggcc atctggatag
     3241 ctgggattcc ggcaccggtg ccgtcgacga cggcgcgggc gtcgccatca ccacggcggc
     3301 ggcaaggctg atcggccagt tgccgcgcca tccccgccgc accatccggg tggtgatgtg
     3361 gggttcggag gaaaccggcg gttccagcgg cgcttatctc gcggcgcata agggcgagtt
     3421 ggcgaagatc gtcgtcgcgt cggaaagcga tctgggttcg gatcgcgtct atcggctgaa
     3481 gctgccgcag ggcgcgctcg cccggcccca gttgaagccg ttggcgggaa tattggcgcc
     3541 gctgaaagtg gccgtctcgc cggagcccgt acagcatgcc ggctcggacg tggagggtct
     3601 gcagaaggcc ggtgttccgg tgttttccct ggatcaggat gccagccgct atttcgattt
     3661 ccatcactcg gcggacgata cgctcgccat cgtggaccgg gcgcagctta accagaacgt
     3721 ggcggcatgg gcggtgctgc tctatctcat cgccgacagc gatgtggatt tccgcgc
//
LOCUS       DASWPU010000476         3734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_35130,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000476 DASWPU010000000
VERSION     DASWPU010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35130"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VGC27_10070"
     CDS             complement(<1..524)
                     /locus_tag="VGC27_10070"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0282954.1"
                     /translation="MNDSIDEKGAAVAHAEKAADILAKVREGIGRVIFGQKDVIDQTL
                     ITLLAGGHGLLIGVPGLAKTKLVEALGTVLGLDWKRIQFTPDLMPADITGSEVLEEAD
                     DRRRAFRFLKGPVFTQLLMADEINRASPRTQSALLQSMQERQVTVAGDRYDLPVPFHV
                     LATQNPLEQEGTYPL"
     gene            682..1308
                     /locus_tag="VGC27_10075"
     CDS             682..1308
                     /locus_tag="VGC27_10075"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY0282955.1"
                     /translation="MDLASLLDKGALRARLLSQPPALPLTPTRGDYDLNPEARRLAPR
                     PLAPASVLLPIVMRAEPSLLFTQRSAHLRRHAGQVSFPGGGAQDGDASLVATALRELK
                     EETGIAAEFVSVQGFLDVYETITGYAVLPVVGLLREGYALAPDANEVAQIFEVPLAFF
                     LDPANRREHVFEWKGAMRRVYAFEVETHYIWGATASMLVNLAERLSVP"
     gene            1305..2525
                     /locus_tag="VGC27_10080"
     CDS             1305..2525
                     /locus_tag="VGC27_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY0282956.1"
                     /translation="MTRLDPQPWMTARPTRKLMAALGEARFVGGAVRNTLLGEPVGDI
                     DIATPLTPDEVGKRLTAAGIPFVPTGIEHGTITAIVQGKPYEVTTLRRDVTTDGRHAV
                     VVFTDDWSLDAQRRDFTMNALYAGADGEVYDFVGGIDDLKAGRVRFVGDAAQRIREDY
                     LRILRLFRFHAWYGKGEIDADALRAAAAEKDGIARLSGERIAKEMLRLLEAEAPAPVL
                     RAMAAAGILAQVLPGEANVARLENLAKIDAANGLAPDAMLRLAALLPHDTKIAAAVAD
                     RWKLSNEARERLADIAGAREEIVPGLSVREARKLLYRLDKRPFKDRVFLRWAEDAGAA
                     AQWRALLALADEWKRPRFPLTGREVMLAGVPEGPKVGKILGEVEEWWIESDFAADEVS
                     LAERLKAALRTAGR"
     gene            2525..3373
                     /locus_tag="VGC27_10085"
     CDS             2525..3373
                     /locus_tag="VGC27_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282957.1"
                     /translation="MPFQLLDSLSLPSDPAKANEDAFAVEKSDAVVLDGTTGLGEMLL
                     PGPSDAAWIAHFGARRLMAHRRDGETPIDALRHALSDAEMSFAGLRRRPPKEKYENPF
                     ASMMFVSAGEKGFEALWFGDCAALVLRPGEGAEIIGEAFEKRTAEARRIKLLAETAGL
                     APAAGLSRAEYLPHLRASRNKVNTDEGGWLFSPDPRAAAHVGLRRVTAPAGTLILLTT
                     DGFLALASDYGAYDAQGLVEAARERGLKALGEELRTLEEADAEGRRFPRLKKSDDATS
                     VLLKMA"
     gene            complement(3374..>3734)
                     /locus_tag="VGC27_10090"
     CDS             complement(3374..>3734)
                     /locus_tag="VGC27_10090"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282958.1"
                     /translation="SVESGPLHDALVPCKNRAAVRRAIIQIAAADISMSLDNVLAVAG
                     AAMQHPYVLVIGLTLSIALMGLAASFVARLLGRYPWISYAGIFIVLFVAVRMIWDGVV
                     RLEETGFFEQAAHMLGV"
BASE COUNT          619 a         1187 c         1291 g          637 t
ORIGIN      
        1 agcggatagg tgccttcctg ctccagcgga ttctgggtcg ccagcacatg aaacggcacc
       61 ggcaggtcgt agcggtcgcc ggccacggtc acttgccgtt cctgcatcga ctgcaacagc
      121 gcagattggg tgcgcgggct ggcgcggttg atctcgtcgg ccatcaaaag ctgggtgaag
      181 accggcccct tgagaaagcg gaaggcgcgg cggcggtcgt cggcttcctc cagcacttcg
      241 gagccggtga tgtcggcggg catcagatcg ggggtaaact ggatgcgctt ccagtccagg
      301 ccgagcaccg tgcccagcgc ttcgaccagc ttggtcttgg ccagccccgg cacgccgatc
      361 aagaggccgt gaccgcctgc aagcagggtg atcagggtct ggtcgatgac gtccttctgg
      421 ccgaagatga cgcggccgat gccttcgcgc accttggcca ggatgtccgc cgccttctcg
      481 gcgtgtgcga ccgccgcgcc tttctcgtcg atcgagtcgt tcataaacgt tccgtatcca
      541 ggttgcaggc ggctttgcgc tccgcctaag cttcatgccg aaaacgcact tggccggaac
      601 gtaccgcgcc ccggcaggcg caacaaggaa aagccgggct tttgcgcccg ctcttgcgat
      661 tcgatgacgg gcgatttttc catggatttg gcgagcctcc tcgacaaggg ggccttgcgc
      721 gcccggctgc tgtcgcagcc gccggcgctg ccgctgacgc ccacgcgcgg cgactacgat
      781 ctcaatcccg aggcgcggag gctggcgccg cgtccgcttg cgcccgcgtc ggtgttgctt
      841 cccatcgtta tgcgcgccga gccttccctg ctcttcaccc agcgcagcgc gcatctgcgc
      901 cgccatgcgg gtcaggtgag ctttcccggc ggcggcgcgc aggacggcga cgcctcgctg
      961 gtcgcgaccg ctttgcgcga actcaaggaa gaaaccggca ttgccgcgga attcgtttcg
     1021 gtgcagggct tcctcgacgt gtacgagacc atcacgggtt atgcggtcct gccggtggtg
     1081 gggctgctcc gcgaagggta cgcgctggcg cccgatgcga acgaagtcgc ccagattttc
     1141 gaagtgccgc tggcgttctt tctcgatccc gccaaccgcc gggaacatgt gttcgaatgg
     1201 aaaggcgcaa tgcggcgcgt ttatgccttc gaagtcgaaa cgcattatat atggggcgcg
     1261 accgcttcga tgctggtgaa tttggcggaa aggctttcgg ttccatgacg cgtctcgatc
     1321 ctcagccttg gatgaccgca cgcccgacgc gcaagctgat ggcggcgctc ggcgaagcgc
     1381 gcttcgtcgg cggcgcggtc cgcaacacgc tgttgggcga gccggtcggc gacatcgaca
     1441 tcgctacgcc gctgacgccg gatgaagtgg gaaaaaggct gacggcggcc ggcattcctt
     1501 tcgtgccgac cggaatcgaa cacggcacca tcaccgccat cgtccagggc aagccttacg
     1561 aggtcaccac cctgcgccgc gacgtgacga ccgacgggcg gcacgcggtc gtggtcttta
     1621 ccgatgactg gagcctcgac gcacagcgcc gcgacttcac catgaacgcg ctctatgccg
     1681 gtgctgacgg cgaggtttac gatttcgtcg gcggcatcga cgatctgaag gcgggccgcg
     1741 tgcgcttcgt cggcgatgct gcccagcgca tccgcgagga ctatctgcgc atcctgcgcc
     1801 tgttccgttt tcatgcctgg tacggcaagg gcgagatcga cgcggacgcc ctgcgcgcgg
     1861 cggcggcgga gaaggacggc atcgccaggc tctcgggcga acgcatcgcc aaggagatgc
     1921 tgcgcctgtt ggaagcggaa gctcccgcgc cggtgctgcg cgcgatggcg gcggccggga
     1981 ttctggcgca ggtgctgccg ggcgaggcga atgtcgcgcg cctggaaaat ctggcgaaga
     2041 tcgacgccgc caatggcctc gcgcccgatg cgatgttgag gcttgcggcg ctgctgccgc
     2101 acgataccaa gatcgcggcg gcggttgccg atcgctggaa gctgtcgaac gaggcgcgcg
     2161 agcggctggc cgacatcgcg ggggcgcgcg aagagatcgt acccggcctt tccgttcgcg
     2221 aggcgcgcaa gctgctctac cgtctggata agcggccgtt caaggatcgg gtgtttctga
     2281 gatgggcgga agacgccggg gccgccgccc aatggcgcgc gctcttggcg ctggcggacg
     2341 aatggaaacg cccgcgcttt ccgctgaccg ggcgcgaggt gatgctggcg ggcgtgccgg
     2401 agggaccgaa ggtgggaaag attctcggcg aagtcgagga atggtggatc gaaagcgatt
     2461 tcgccgccga cgaagtttcg ctggccgagc ggctcaaggc ggcgctgcgg acggcggggc
     2521 gctgatgccg ttccaactgc tcgactccct ttcgcttcct tccgatccgg ccaaggccaa
     2581 cgaagacgcc ttcgccgtgg agaaatccga tgccgtggtg ctcgacggca ccaccgggct
     2641 tggcgagatg ctgctgccgg ggccttccga cgcggcctgg atcgcccatt tcggcgcgcg
     2701 ccggctgatg gcgcaccgcc gcgacggcga gacgccgatc gacgcgttgc gtcacgctct
     2761 ctccgacgcg gagatgtcgt ttgcgggatt aaggcggcgt ccgcccaagg agaaatacga
     2821 gaatccgttc gcctcgatga tgttcgtgtc ggccggcgaa aaaggtttcg aggcgttgtg
     2881 gttcggcgat tgtgcggcgc tggtgttgcg tccgggcgag ggcgccgaaa ttatcggcga
     2941 ggcgttcgag aagcgcacgg ccgaagcgcg ccgcatcaag ctgctggccg aaaccgctgg
     3001 actggcgccc gccgccggcc tgtcgcgcgc cgaatatctg cctcatctgc gcgcttcgcg
     3061 gaacaaagtg aacacggacg agggcggctg gctgttctcg cccgatccgc gcgccgccgc
     3121 gcatgtcggc ttgcggcgcg taaccgcgcc ggcgggcacg ctcattctgc tgacgacgga
     3181 cggcttcctg gcgctggcca gcgactacgg cgcctatgac gcgcaaggac tggtcgaggc
     3241 ggcgcgggaa aggggcctca aggcgctggg agaggaattg cgtacgctcg aagaagccga
     3301 cgccgagggc cgccgcttcc cgcggctgaa aaagagcgac gacgccactt cggtgttgct
     3361 gaagatggcg tgatcacacg cccagcatat gggcggcctg ttcgaagaat ccggtttcct
     3421 caagccgcac cacgccgtcc caaatcatcc tgaccgcgac gaagagcacg atgaagatgc
     3481 cggcgtagct gatccaggga tagcggccca gcagcctggc cacgaagctc gccgccagac
     3541 ccatcagggc gattgacaag gtgaggccga tcaccagcac ataagggtgc tgcatcgcgg
     3601 cgcccgcaac ggccagcaca ttgtccagcg acatggaaat gtctgcggcg gcgatttgga
     3661 tgatggcgcg ccgcaccgcc gcccgatttt tgcagggcac gagggcatcg tgcagcgggc
     3721 cggattccac cgac
//
LOCUS       DASWPU010000477         6417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_41412,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000477 DASWPU010000000
VERSION     DASWPU010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41412"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2845)
                     /locus_tag="VGC27_10095"
     CDS             complement(<1..2845)
                     /locus_tag="VGC27_10095"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY0282959.1"
                     /translation="MVLAVSNSDHDRQTKALLAQSSETDPVLRAFFAALYRNGAPEDL
                     SRYAPQELAALARLVFARAVKHKPGTTLVTLFDPAAEAPAYTHNETVLAAVNDDSPFL
                     FDSLLGEVTALGVRLRAVFHPVIAMAGQPTSIIVLVFDMLVGEDRRGALVRGAESVFA
                     QVRVAVRDWQPMQERLKDAVVRLKAQPPVAAGELAESLAFLEWLGDNHFTFLGCRDYV
                     FSDEDGGRLDPVEGSGLGVLADSDALVLDKRPDRARLTPPVRDFLTQRTPLIVAKSNQ
                     QSLVHRRVGMDYIGVKFFDPGGALLGERRFVGLFTSGAYSRRPADIPLLRRKVETVMA
                     RAGLAPSSHDAKTLAHILDTFPRDELFQISDDELHTIALGILRLSERPKVRAFVRFDL
                     FDRFVSSLVFVPRERYGGQAGEKIHAILARAFNGRPVAATPTLDESPLARIHYLVARN
                     EGPRPSVDIGALEEEIRGAVRTWDDGFSEALRARYGEIEGLHLYHDQTEGFPARYRDA
                     FSPEEAVEDLAALRALARVPRAPAIEARVLRKPHDAHSVLRIKLYGLGSALPLSVSLP
                     IFENFGLKVIAEDAYTVALKTDDGPLRVAFVLDFLMERADQGPADLAEIKGPLELAFH
                     AVVAGEAESDGFNRLVIGAGLDWRDVTILRAAAKFLRQAAIQFSQDYMEQALAHNPDI
                     ARLLVALFHASCAPGGTGAETLHKKIEAALSDVQSLDDDRIIRRLRNVVANVLRTNFY
                     QPDENGRPKSYLAFKLDSEKLDELPAPRPQVEIFVYSPAVEGVHLRFGKVARGGIRWS
                     DRREDFRTEVLGLVKAQQVKNAVIVPVGAKGGFYPKRLPAGATREEVQAAGIAAYKIF
                     INALLDVTDNLHPDGSVAPPENVVRRDEDDPYLVVAADKGTATFSDIANGVAADYGFW
                     LDDAFASGGSVGYDHKAMGITARGAWEAVKRHFR"
     gene            complement(2932..3615)
                     /locus_tag="VGC27_10100"
     CDS             complement(2932..3615)
                     /locus_tag="VGC27_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282960.1"
                     /translation="MVAYARMLTASETAGRESGEPERQSDQPGGITKLAQLYAEAEET
                     ARLANLLGRSLYAAIALPLLGLFAVALAGGTGVARPLVWCAFVAAAAIAMLRSYFHTI
                     GQSFERAALKSFAKDMSPILLFAGFAWGAGAFLALPAGTGTAAAILFAAAPGIAIAAL
                     LREREAVFLFLAPVATLAAFASVLRPFAGGALAAGLILIACAAVAAAVVLWDRRDGDF
                     VRPAMLPLA"
     gene            3713..4306
                     /locus_tag="VGC27_10105"
     CDS             3713..4306
                     /locus_tag="VGC27_10105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY0282961.1"
                     /translation="MVSSQQIRSSAAVVFDLESTAWEGSMEAGWLRPGEFQEVVQIGA
                     VKVDEKFNAGETFDMLVRPRINPVLSPYLERLTGLTNEAMRERGVDFAEAYRAFLAFC
                     DGLPVVAFGRDDLVFADNLRLYGISDAPPLPPCLNAKTWMAELGLDMRGLHACDVASS
                     AGVPFGGHRHNGLDDALSVAAGIAALIARGAPAPLAA"
     gene            4303..4728
                     /locus_tag="VGC27_10110"
     CDS             4303..4728
                     /locus_tag="VGC27_10110"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0282962.1"
                     /translation="MTGPSRQGEAERIIAALELSSHPEGGHYRETFRDGAGSSGRAHS
                     TAIYFLLREGEVSAPHRIDAAEVWHFYRGAPLELTIREEGKAAVRLVLGPDIEKGERP
                     QIVVPPRAWQGARSLGAYTLVGCTVAPGFEFAAFELAPP"
     gene            complement(4893..5657)
                     /locus_tag="VGC27_10115"
     CDS             complement(4893..5657)
                     /locus_tag="VGC27_10115"
                     /inference="COORDINATES: protein motif:HMM:NF027577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4908 domain-containing protein"
                     /protein_id="HEY0282963.1"
                     /translation="MPKRGFALLLCALLSFALAKRAGAEVYDRIEQQHFGSITTGSYQ
                     AGDNLRFSIDHYRNEFLMRFTGQPEVFVLYADYGSLGGRVLRYDSGAIAIQVAGWGGV
                     TVYTDNQPEGLPATRTGNSSGPSLPSVTLAQAQSAAEDEAAHLLYARNVRVSFSADWN
                     SLSSDSSLRAMAFDAMENAARAIDRFTAIPAARAAFTQKVSAVQMQQTGDRPKIQLTG
                     KTLIVTFNPRQGYKGRASSRAIAFALGKLFGVPVPN"
     gene            5927..>6417
                     /locus_tag="VGC27_10120"
     CDS             5927..>6417
                     /locus_tag="VGC27_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY0282964.1"
                     /translation="MTSSLNTFKSRRTLTAGGKTYVYFSLAAAEKNGLTGISRLPYSL
                     KVLLENLLRHEDGKTVTAADIKAVAGWLADKTSDREIAFRPARVLMQDFTGVPAVVDL
                     AAMRDAMKALGGDPEKINPLAEVDLVIDHSVMVDNFGGRDSFKKNVAVEYERNMERYA
                     FLR"
BASE COUNT         1144 a         2015 c         2169 g         1089 t
ORIGIN      
        1 cgcggaagtg ccgtttgacg gcttcccacg cgccgcgagc ggtgatgccc atggccttgt
       61 ggtcgtagcc gaccgacccg ccggaggcaa aggcgtcgtc cagccagaag ccatagtcgg
      121 ccgccacgcc attggcgatg tcggagaagg tggccgtgcc cttgtcggcg gcgaccacca
      181 gataaggatc gtcctcgtcg cggcgcacga cgttctcggg cggcgccacg ctgccgtcgg
      241 gatgcagatt gtcggtgacg tcgagcaggg cgttgatgaa aatcttgtag gcggcgatgc
      301 cggccgcctg gacttcttcg cgcgtggcgc ccgccggcaa tcgcttggga tagaagccgc
      361 ccttggcgcc caccggcacg atgacggcgt tcttcacctg ctgcgccttg accaggccga
      421 gcacttcggt gcggaaatcc tcgcgccggt cgctccagcg gatgccgccg cgcgccacct
      481 tgccgaagcg cagatgcacg ccctcgaccg cgggcgaata gacgaaaatc tccacctgcg
      541 ggcgcggcgc gggcagttcg tcgagctttt cggagtccag cttgaaggcg aggtaggatt
      601 tgggccggcc gttttcgtcg ggctgataga aattcgtgcg cagcacattg gcgacgacgt
      661 tgcgcaggcg gcggatgatg cggtcgtcgt ccaggctttg cacgtctgag agcgccgctt
      721 cgatcttctt gtgcagcgtc tcggcgccgg tgccgccggg ggcgcagctt gcgtggaaca
      781 gcgcgaccag cagccgggcg atgtcgggat tgtgcgccag cgcctgctcc atatagtcct
      841 ggctgaactg gatcgcggcc tggcgcagga atttcgcggc cgcgcgcagg atcgtcacgt
      901 cgcgccaatc cagacccgcg ccgatcacca gccggttgaa gccgtcgctt tcggcttcgc
      961 cggcgacgac ggcatggaag gcaagttcga gcggcccttt gatctcggcg agatcggcgg
     1021 ggccctgatc ggcgcgttcc atcaggaagt cgagcacgaa agcgacgcgc agcgggccgt
     1081 cgtcggtctt gagcgcgacg gtgtaggcgt cctcggcgat cactttcagg ccgaaattct
     1141 cgaagatcgg cagcgacacc gaaagcggca gcgccgatcc cagcccgtaa agtttgatgc
     1201 gcagcacgct gtgggcgtcg tgcggcttgc gcaggacgcg cgcctcgatc gcgggcgccc
     1261 tgggtacgcg cgccaaagcg cgcaaggcgg cgaggtcctc caccgcctct tccggcgaaa
     1321 aggcgtcgcg atagcgcgcc ggaaagcctt cggtttgatc gtgatagagg tgcagccctt
     1381 cgatttcgcc gtagcgcgcg cgcagcgcct cggagaaacc gtcgtcccag gtgcgcacgg
     1441 cgccgcggat ttcttcttcc aaggcgccga tatcgacgga ggggcgcggg ccttcgttgc
     1501 gcgcgacgag ataatggatg cgcgccagcg gggattcgtc gagcgtgggc gtggccgcta
     1561 ccggccttcc gttgaaggcg cgcgccagga tggcgtggat cttctctccc gcctggccgc
     1621 catagcgttc gcgcggcacg aagacgaggc tggagacgaa acggtcgaaa aggtcgaagc
     1681 ggacgaaggc gcgcaccttc ggccgttcgc tcagccggag gatgccgagc gcgatggtgt
     1741 gaagctcgtc gtcggaaatc tggaacagtt cgtcgcgcgg aaaggtgtcg aggatgtggg
     1801 ccagcgtctt ggcgtcgtga ctggacggcg ccaggccggc gcgcgccatc accgtttcca
     1861 ccttgcgacg cagcagcgga atgtcggccg gccggcggct ataggcgccc gaggtgaaca
     1921 ggccgacgaa gcgccgctcg cccagcaagg cgccgccggg gtcgaagaat ttgacgccga
     1981 tatagtccat gccgacgcgg cggtggacca ggctttgctg gttcgatttg gcgacgatca
     2041 gcggcgtgcg ctgggtcagg aaatcgcgca ccggcggcgt cagacgggcg cggtcaggcc
     2101 gcttgtccag caccagcgcg tcgctgtcgg cgagcacgcc gaggccgctg ccctccaccg
     2161 gatcgagcct gccgccgtct tcatcggaaa aaacgtagtc gcggcagccc aggaaggtga
     2221 aatgattgtc gcccagccat tcgaggaagg cgaggctttc ggccagttcg ccggcggcga
     2281 cgggcggctg cgccttgagg cggacgacgg cgtccttcaa ccgctcctgc atcggctgcc
     2341 agtcgcgcac cgccacacgc acctgcgcga agacggactc ggcgccgcgc accagcgcgc
     2401 cccggcggtc ctcgccgacg agcatgtcga agaccagaac gatgatgctg gtcggctgcc
     2461 ccgccatggc gatgacggga tggaacacgg cgcgcagacg cacgcccagg gccgtgacct
     2521 cgcccagcag ggaatcgaac aggaacggcg agtcgtcgtt gaccgcggcc agaaccgttt
     2581 cgttgtgcgt ataggccggt gcttccgcgg cgggatcgaa cagggttacg agcgtcgtgc
     2641 cgggcttgtg tttgacggcg cgcgcgaaca ccagacgcgc cagcgcggca agttcctgcg
     2701 gcgcatagcg gctgagatct tccggcgcgc cgttgcggta aagcgccgcg aaaaaggcgc
     2761 gcagcacggg atcggtttcg ctgctttgcg ccagcagcgc ttttgtctgc cggtcatggt
     2821 cggaattgct gacggcgaga accatagggt gcccggtgcg aggaaggcga acgcgcctcc
     2881 gcacagttta agctaccgcg attgcgctta aaacccttcg ccgctgggtg cttacgctaa
     2941 aggcagcatg gcaggcctga cgaaatcgcc gtcccggcga tcccacagca cgactgcggc
     3001 ggcgacggcc gcgcaagcga tcaggatcaa tcccgcggca agcgcgccgc ctgcaaacgg
     3061 tctcagcacg ctggcgaaag cggcaagcgt tgcgaccggc gcaagaaaca gaaatacggc
     3121 ttcgcgttcg cgcaacaaag cggcaatggc gatgcccggc gccgcagcga acagaatggc
     3181 ggccgccgtg ccggttcccg ccggcagcgc caggaacgcg cccgcacccc aggcgaagcc
     3241 tgcaaagagc aggatggggc tcatgtcctt ggcgaaggat ttcaaggccg cgcgctcgaa
     3301 ggactggccg atcgtatgga aataggagcg cagcatggcg atcgccgcgg ccgccacgaa
     3361 ggcgcaccag accagcggcc gcgccactcc cgtgcctccg gccaaggcga cggcgaagag
     3421 acccagcaaa ggcaaggcga tggcggcgta aaggctgcgt ccgagcaggt tggccagccg
     3481 cgccgtttct tcggcttcgg catataactg cgccagcttg gtgatgccgc cgggctgatc
     3541 gctctggcgt tcgggttcgc cggattcgcg tccggcggtc tccgatgccg tcagcatgcg
     3601 cgcatatgca accatgaggc tctccgtcgc agaaataacg cgataccagc ctgggtgtgg
     3661 accgttaaga atgtcttaaa tttgccggca agacgcgatt cgggtgacgc caatggtcag
     3721 ttctcagcag attcgttcgt ccgccgcggt ggtgttcgat ctcgaatcca ccgcctggga
     3781 aggctcgatg gaggccggct ggctgcggcc cggcgagttt caggaggtcg tgcagatcgg
     3841 cgcagtgaag gtggacgaga aattcaacgc cggcgaaacc ttcgacatgc tggttcgccc
     3901 gcggatcaat ccggtgctgt cgccctatct cgaaaggctg accggcctga ccaacgaagc
     3961 gatgcgcgaa agaggcgtgg atttcgccga agcctatcgc gccttcctgg cgttctgcga
     4021 cggcttgccg gtcgtcgcct tcggccgcga cgatctcgtg ttcgccgata atctcaggct
     4081 ctacggcatc agcgacgcgc cgccgctgcc gccctgcctc aacgccaaaa cctggatggc
     4141 ggagttgggg ctcgacatgc gcggtctgca cgcctgcgac gtggcgtctt cggccggtgt
     4201 gccgttcggc gggcaccggc acaacggcct cgacgatgcg ctgtcggtgg ccgccggtat
     4261 cgctgccctg atcgcccgcg gcgcgcctgc tccgcttgcc gcatgacggg gccgagccga
     4321 caaggcgagg ccgagcgcat catcgcggca cttgagcttt cgtctcatcc cgaaggcggg
     4381 cattaccgcg agaccttccg tgacggcgcc ggatcgtccg gccgcgccca ttcgacggcg
     4441 atctactttc tgctgcgcga aggtgaggtc tcggccccgc atcggatcga tgcggccgag
     4501 gtctggcatt tctatcgcgg cgcgccgctg gaattgacga tccgcgaaga aggcaaagcg
     4561 gccgtgcgcc tggtgctggg gccggatatc gagaagggcg aacgcccgca aatcgtggtg
     4621 ccgccaaggg catggcaagg cgcgcggtcc ttgggcgctt acacgctggt gggctgtacg
     4681 gtggcgcccg ggttcgagtt tgccgctttc gaactggcgc cgccttagaa gggcatccga
     4741 ccagatcgga ccgtgccgct gccatgcttc gacaagctca tgcttcgaca agctcatact
     4801 tcgacaagct cagcatgagg ggttgtcttc aaccttcacc ctgagcctgt cgaagggtgc
     4861 cgagaaacga ttccataaga ccggatggcg ccctaattcg gcaccggcac gccgaacagc
     4921 ttgcccagcg cgaaggcgat ggcgcgcgaa gaggcgcgtc ccttatagcc ctggcgtggg
     4981 ttgaaggtca cgatcagcgt cttgccggtt agctggatct tcggcctgtc gccggtctgc
     5041 tgcatctgca ccgcggacac tttctgggtg aaggcggcgc gcgccgccgg gatcgccgtg
     5101 aagcggtcga tggcgcgggc ggcgttttcc atcgcgtcga atgccatcgc gcgcaagctg
     5161 gagtcggagg acagcgaatt ccagtcggcc gagaagctca cgcgcacgtt gcgcgcatat
     5221 aacaggtggg cggcctcgtc ttcggcggcg ctttgcgcct gggcgagagt gaccgaaggc
     5281 aggctagggc ccgaggaatt gccggtacgc gtggcgggca ggccttcggg ctgattgtcg
     5341 gtgtagaccg tcacgccgcc ccagccggcg acctggattg cgatggcgcc ggaatcgtat
     5401 ctgagcacgc gtccgccgag cgagccatag tcggcataga gcacgaagac ttccggctgt
     5461 ccggtaaagc gcatcaggaa ttcgttgcga tagtggtcga tggagaaccg cagattgtcg
     5521 ccggcctggt agctgccggt cgtgatcgaa ccgaaatgct gctgttcgat gcgatcgtac
     5581 acttcggcgc ccgcccgctt cgcaagcgca aaggaaagaa gcgcgcaaag aagaagagcg
     5641 aacccgcgtt tgggcatctg atgtcgtctt ctcgttttgc cgccatgcaa ccggcacatt
     5701 gccccgacgc gttcccttgg ccgcaaattt aacagaagga atagggcgat tttatgccct
     5761 caggcgttaa agaaagtctc ccggcggcta ctaaagcttc cgaatccctt gttgtaacaa
     5821 cgcaaattgt cacgcagggc cggctcaccc gttcaaacaa ccattggcag cgggcaagaa
     5881 acttactaaa ccacgtcttc cccaacccca ttgcaaaggc actttcgtga cttcgagcct
     5941 caacaccttc aaatcccgcc gcaccctgac ggcgggcggc aagacctatg tctatttcag
     6001 tctggcggcg gcagaaaaga acgggcttac gggcatctcc cggctgcctt attcgctgaa
     6061 ggttctgctc gagaacctgc tccgccacga ggacggcaag acggtgaccg cggccgacat
     6121 caaggcggtg gccggatggc tcgccgacaa gacatccgac cgcgaaatag ccttccgccc
     6181 ggcgcgcgtg ctgatgcagg actttaccgg cgttcccgcc gtggtcgatc tggcggcgat
     6241 gcgcgatgcg atgaaagcgc tgggcggcga tcccgaaaag atcaacccgc tggccgaggt
     6301 cgatctggtg atcgaccatt cggtgatggt ggacaatttc ggcggccgcg attcattcaa
     6361 gaagaacgtc gcggtggaat acgaacgcaa catggaacgc tatgcgttcc tgcgctg
//
LOCUS       DASWPU010000478         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_108481,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000478 DASWPU010000000
VERSION     DASWPU010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108481"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1675..2586)
                     /locus_tag="VGC27_10125"
     CDS             complement(1675..2586)
                     /locus_tag="VGC27_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0282965.1"
                     /translation="MAIRFNGKVAIVTGAGGGLGRAHALEFAKRGAKVVVNDPGGALD
                     GTGGNSQAAEAVVAEIKAAGGIAIANGASVADDAGVAHLVKQTMEAFGRIDILVANAG
                     ILRDKSFGKMELKDFEAVMEVHLMGTVKPCKAAWPIMREQQYGRIVVTTSSTGLYGNF
                     GQTNYGAAKASLIGFMNSLKLEGAKDNIKVNAVCPVAGTRMTENLMPPNLLEMLKPEY
                     VTPGVVFLASDGAPSGAILTAAAGVFAAARIEETDGVHLGQKAGADDVAANWSKIADF
                     STAKYYSQGSEQFQKLFARLQDPPVVG"
     gene            complement(2655..3881)
                     /locus_tag="VGC27_10130"
     CDS             complement(2655..3881)
                     /locus_tag="VGC27_10130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0282966.1"
                     /translation="MRPSTRPPEIKALSGARALPPLILVLYHFCEGHGYRGADLPACI
                     GHPWVAFFQCGTWFDLPVGKGYLWVEFFFALSGFILIYIYGARAQEFWRGKAYLPFLT
                     ARLSRLYPLHLFLLFAMLLLMWTLNWLSYLGGYISIYHQPYPPMNTWPSFVANLFLVH
                     AWNLFPWLTWNSASWFVSVEFFLCLLFPIYVLLSRGGVLRALALIAAGVAVLTFIAQN
                     SGNGLDVTFHNGVVRGMAGFAVGVGMAMLYRSAKEAGAERLPERFWSAVQVLVLVFLY
                     WATYHTGWSHHPNDIWFALALYALIFAIAFDRGFLARAFAARPLLKLGEWSYAIYMGQ
                     IFFLQLIRTLEQRLYPPPDTMIFGWRFADLMWWAEPFALLLVCVGWGALLAIYIEHPA
                     NAALRKLFAPRKTASA"
     gene            3939..>4175
                     /locus_tag="VGC27_10135"
     CDS             3939..>4175
                     /locus_tag="VGC27_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HEY0282967.1"
                     /translation="MLSQFQSLLGSSGGVLSHFQASLQSLFGAGVLPHFQSLLGIAVI
                     LLIAWIISENRRGFPFRTVISGIVLQITLALLLLK"
BASE COUNT          819 a         1316 c         1269 g          771 t
ORIGIN      
        1 tgcgggaaat cccgcctaaa agcgccgcaa cggcaagtaa aatggggaat ggtccgccgc
       61 tgcgccttga cgaccgctat cttatcttca tgcgttttgc ataaagcgaa gcgccggatt
      121 gaaaagccgc cgtgaccttt cggtgacgcg caagcgggat cgagcgtcat cttgccggcc
      181 ggtgtcgagg tgcaatcgtc gatcgcggtg tgggccgtga accatgcgca agcggtacag
      241 tacgccgccg cacctgcgcg cggcgaggga gtcaccctcc cggcctctga agcgcaggcg
      301 ctgtcttgaa gggtagccgc acatcttccc acgagcacca gatgcaccgt ggagtgccgc
      361 tgcatcacgc cgcaaaagaa ctcaacgcca agcattcgag ccatgttggg gctacgacag
      421 gctgctcaaa aaagcagcga actgacccgc acttttttcg aatgcttctg cttctcggtt
      481 tgcgccgcga acctagccgg agacaccggc atgatcagac gcgcggcgtc ctcttatccg
      541 agattaaaaa gcgaggtgta aacgaaatac cccacgccaa cgaacaaggc caaagtgagg
      601 attccggcac cgatcgattt tccgaactct gagatcgaat ccattgttcc tctccgttac
      661 ttggtcggtc gcggctttag tggccgcgcg ttaaccaacc atcatttgag aggataagcc
      721 ggtcacattt gcgccacaat gcttttcagc aaatttccgg agatggacgg gcatgcagta
      781 tgggtatgcg aagaagccag tgtccggatg aagccttcag gcggccgcat ggctgaattt
      841 cctgcaacat gatgttccct ggccccattt gctgcaccgc ggcacgcgcc gccatggggt
      901 aggcgggctt atgaagcccg cctgtttccc acgactgtca ctgtttcgat ggcagggctg
      961 ctggtctgca cgctggtgac ttccacggcc ggcttcggct gggggacaac caaggtgctt
     1021 gccaataagc tgccaacgcc gccgattagg gccaccgtga gcaaacccgc actaaacatt
     1081 ttcacagact ctttcataac cctctccttg tttggggctg gccgcggcct ggtcgccgcc
     1141 ggatcgccag caatggtcga tagatagggt gcgacagcct gtccgcaata gttttatgct
     1201 gcgccgcagc agatggtggc tatgcagaga cggcatggtg caaaaccttg aatttcgtgc
     1261 tggccgcact actcgccact taacaatagt gtcatatgtt ttttctggtc cgggccgtcc
     1321 cgacgtgccg cgatccttac atcagggact tcgattacga ccgcgccatc gacatcgcca
     1381 acaacgccaa ggccatcaac gtctgttgag ggctgctgcg gtctctctct cgttctagag
     1441 caaaatgcat tcagacggaa tcgccccaca tccttcggca agctctgggt gagggcattt
     1501 tctaatccct catgctggtc ggcacgctgt tgctaccgac tgtcgaagca tgagcttgcc
     1561 cgccttcgct gcgctacggc ggaccaaagc tctaatggcc cgcccgccgg agctttagcg
     1621 taggcgggtc gaaccatgag cttatcgaag catgagggtg ccgtaaccag tacactaccc
     1681 caccaccggc gggtcttgca gccgggcgaa gagcttctgg aattgctccg aaccctgcga
     1741 atagtatttt gcggtagaga aatccgcgat cttcgaccag ttcgcggcga cgtcatcggc
     1801 gcccgctttc tggcctaggt gaacgccgtc ggtttcctcg atccgcgccg ccgcgaacac
     1861 gcccgccgcc gcggtcagga tcgcgccgct cggcgcgccg tcgctcgcca gaaacaccac
     1921 gcccggcgtc acatattccg gcttcagcat ttcgagcagg ttgggcggca tcaggttctc
     1981 ggtcatgcgc gttcccgcca ccgggcagac ggcgttgacc ttgatgttgt ccttggcgcc
     2041 ttccagcttc agcgaattca tgaagccgat cagcgacgct ttggccgcgc cgtaattggt
     2101 ttggccgaaa ttgccgtaaa ggccggtgga ggaggtcgtc accacgatgc ggccatattg
     2161 ctgctcgcgc atgatcggcc acgccgcctt gcacggcttg accgtgccca tcaggtgaac
     2221 ctccatcacc gcctcgaaat ccttcagctc cattttgccg aagctcttgt cgcgcaggat
     2281 gccggcgttg gcgaccagga tgtcgatgcg cccgaacgcc tccatcgtct gcttgaccag
     2341 atgggcgacg cccgcatcgt cggccaccga ggcgccgttg gcgatggcga tgccgcccgc
     2401 cgccttgatc tccgcgacca cggcttccgc cgcctggctg ttgccgccgg tgccgtccag
     2461 cgccccgccc ggatcgttga ccaccacctt ggcgccgcgc ttggcgaatt cgagcgcatg
     2521 ggcgcggccc agccctccgc ccgcgccggt gacgattgcg actttgccgt tgaagcgaat
     2581 ggccatgaca agtcctcccg gtcggtgaaa atcgttcgga tcagtgatcg cgcggcaaag
     2641 gcccggcggt caagttaagc gctcgccgtt tttcgcggcg cgaacagttt cctcagcgcc
     2701 gcgttggccg gatgctcgat gtagatcgcc agcagcgccc cccagccgac gcagaccaga
     2761 agcagcgcga acggttcggc ccaccacatc agatccgcga agcgccagcc gaaaatcatc
     2821 gtgtcgggcg gcggatagag gcgctgttcg agagtgcgga tgagctggag gaagaagatc
     2881 tggcccatat aaatcgcata ggaccattcg ccgagcttga gcagcgggcg cgcagcgaac
     2941 gcgcgcgcca ggaagccgcg atcgaaggcg atggcgaaga tcagcgcgta gagcgccagc
     3001 gcgaaccaga tgtcgttggg gtggtgcgac cagccggtgt ggtaggtcgc ccagtagagg
     3061 aagaccagca ccagaacctg caccgccgac cagaaccgtt ccggcagccg ctcggcgccg
     3121 gcctcttttg cgctgcggta cagcatcgcc atgcccacgc cgacggcgaa cccggccatg
     3181 ccgcgcacca cgccgttgtg gaaggtgacg tcgagcccgt tcccggagtt ttgcgcgatg
     3241 aaggtcagca ccgccacgcc cgccgcgatc agcgccagcg cgcgcagcac gccgccgcgc
     3301 gacagcagca catagatcgg aaacaagagg cacagaaaga attcgacgct gacgaaccac
     3361 gacgcgctgt tccaggtcag ccagggaaac aggttccatg cgtgcaccag gaagaggttg
     3421 gcgacgaagc tcggccaggt gttcatcggc ggatagggct gatggtagat cgagatatag
     3481 ccgccgagat aggacagcca gttcagcgtc cacatcagca gcagcatggc gaacagcagg
     3541 aacaggtgca gcggatagag ccgcgacagc cgcgccgtca ggaacggcag ataggccttg
     3601 ccgcgccaga actcctgcgc ccgcgcgccg tagatataga tcaggatgaa gcccgacagc
     3661 gcaaagaaga actccaccca cagatagccc ttgccgaccg gcaggtcgaa ccacgtgccg
     3721 cattggaaga acgccaccca gggatggccg atgcaggcgg gcaggtcggc gccgcgataa
     3781 ccgtgcccct cgcagaagtg gtagagcacc aggatcagcg gcggcagcgc gcgcgcgccg
     3841 gaaagcgcct tgatctccgg ggggcgggta gagggtcgca tggcgcggct ttcgaatcgg
     3901 cgtagaagtg taggctgcgg tcaagcggtg gggccagcgt gctgtcacaa ttccagtctc
     3961 tgctcggttc gagcggcgga gtgctttcgc atttccaggc gtcgctccaa tccctgttcg
     4021 gcgcaggcgt gcttccgcac ttccaatctc ttctcggaat cgcggtgatc ctgctgatcg
     4081 cgtggatcat ctcggaaaac cgccgcggct ttccgttccg caccgtgatc tcgggcatcg
     4141 tcctgcagat cacgctggcg ctgctgcttt tgaag
//
LOCUS       DASWPU010000479         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_3735, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000479 DASWPU010000000
VERSION     DASWPU010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3735"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1607)
                     /locus_tag="VGC27_10140"
                     /pseudo
     CDS             complement(1..1607)
                     /locus_tag="VGC27_10140"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          321 a          526 c          506 g          263 t
ORIGIN      
        1 tcatgccaga cgccgcgcag cgcctgactc agcagcgcct ggtcggcgca tttgggtgtg
       61 accaggaacg gcttgccgct ggtgaacagg cccgtccaca cggccttgcc gacggtgtga
      121 gtcagcgaca ggccggcgac cgaggccatc agcgcaccct tgacccccga accgaccttg
      181 ttgggataga gcagcagcgt cttcaaggtc ttggccaaga acagcgccag cgccgccgcc
      241 gacaaggccg gcatgggcac atagacgtaa cgcggcgccg cccacatcat gaaggtccag
      301 accagcgcca tcagggtgac gaccatgccc accccgtcgg aaatccacgg cagccagccg
      361 ctgagaaact gataacgctg cgcccaggac agccgcgccc tgcccgcgaa aatcgcgccc
      421 ttgtgacgtt tcatgatctg catggcgcca tagacccagc ggtagcgctg gctctggaac
      481 gccttcagcg tgtcggggat caggcccttg cccatgctcc gcgggatgta ggccgcgcca
      541 taccccgctt cgaacagcct gagccccagt tcggtgtcct cggtgatgca ccacatcgcc
      601 cagccgccga cctcgtccag cgcgtccttg cggacgatcg tcatagtgcc atgctggatg
      661 atggcgttgt actcgttgcg ctagaccatg ccgatctgga agaagccgcg atactcctcg
      721 aacgccatcg ccttgaacag gctgtccgag gcgtcggcgt aatcctgcgg accctgcacc
      781 agcgcggtgc cggggtcggc gaagtacggc aagctcaagc gcagccaatt gggatcgacg
      841 cgatagtcgc tgtcgatgac ggcgatgtat ttcgcgcggg gatcggtcag ctccagcgcc
      901 agattgagcg cgccggcctt gaagccccgt accttgtcgt aatgatggaa gcggaagcgc
      961 ggaccgagcg ccgcgcaata ggcttccacc ggcttccagg tcgcgggatc gggcgtgttg
     1021 ttgtcgagga cgaggacttc gaaattgtcg taatcgagcc gcgccagcgc atcgagggtt
     1081 tcgatcacca tcgccggcgg ttcattgtag caaggcacgt gcaccgagac gcgctcgctc
     1141 gcctcgggaa tgctggcgcg catggcgcgg cgctccaccc gccacaggct ggaggccagc
     1201 tcgatgccct cggtcaggat gacggtggaa gcgagaaaaa ccagaggcga catcgccgcg
     1261 atcatgacga tatcggcggg atcgatgtaa tcgagagtgg tggcgtcgat caccagcaac
     1321 aggcccgtcg tgacaaacgc caccagtccg cccatgacga tatagcccgt ctggcgcacg
     1381 cgcggcatgc gccccaggat gaacaatccc agcagcagcg tgaagatggc cgccccgaac
     1441 atgaaactgc gccactcggg gaaggagcgc agcataccgg agaaagcgaa tttgggtttg
     1501 ccgccggcgt cgaacagccc ccaataggcg cccaccatgc cttcgctgcc ggccttccaa
     1561 ggctgatcgt aggcttcgat gatgtaatag tcgtaaccct tgtccatcgc gagctg
//
LOCUS       DASWPU010000480         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_28292,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000480 DASWPU010000000
VERSION     DASWPU010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28292"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VGC27_10145"
     CDS             complement(<1..611)
                     /locus_tag="VGC27_10145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HEY0282968.1"
                     /translation="MNGFGLYVHWPFCAAKCPYCDFNSHVRASIDEEGWAQAILREMT
                     HVAELQSAELPVVQTIFFGGGTPSLMSARSVGRIIDAATGLLPSANDVEITLEANPAS
                     AEAARFADYAVAGVNRLSLGIQALNDADLHFLGRLHDVREAKAALALAQQTFKRVSLD
                     LIYARPGQSLKAWRSELDEALAFGTEHLSLYQLTIEPQTPFAT"
     gene            complement(608..1210)
                     /gene="rdgB"
                     /locus_tag="VGC27_10150"
     CDS             complement(608..1210)
                     /gene="rdgB"
                     /locus_tag="VGC27_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY0282969.1"
                     /translation="MKLERGSVLLVASHNRGKVREIADLLSPFAIAVRGAAELGLAEP
                     EETGATFAGNALLKARAAAEASGLAALADDSGLCVAALDGAPGIYSARWAGPEKDFAL
                     AMARVERELKTKGAKDRSAKFVCVLALAAPSGETEIFEGEVRGELVFPPRGTNGFGYD
                     PIFVAEGMERSFGEIDPAQKHAISHRARAFEKFARAVLAP"
     gene            complement(1207..1920)
                     /gene="rph"
                     /locus_tag="VGC27_10155"
     CDS             complement(1207..1920)
                     /gene="rph"
                     /locus_tag="VGC27_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009584291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY0282970.1"
                     /translation="MRHERALDQLRKVSLEPGFAKHAEGSCLVKFGDTHVLVTASLEE
                     AIPSFLRGSGKGWVTAEYGMLPRSTHERMRREAATGKQSGRTQEIQRLIGRSLRAVVD
                     LSALGERQITIDCDVLQADGGTRTASVTGGFVALAGCIAFMKKTGMVTKPAIKDHVAA
                     ISCGIVKGQVMLDLDYAEDSAAETDANFVLTGSGGLVEIQGTAEGAPFSDEQFAQLLV
                     QARKGIAELVALQKAALGQ"
     gene            2034..>2462
                     /locus_tag="VGC27_10160"
     CDS             2034..>2462
                     /locus_tag="VGC27_10160"
                     /inference="COORDINATES: protein motif:HMM:NF015409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282971.1"
                     /translation="MAPSRANTEFLADRQREVFRRLVEAFLASGEPVGSRTLSQLLPI
                     ALSPASIRNVMADLETMGLLTAPHTSAGRVPTEKGLRLFVDGLLQIGELAPAERASIE
                     ARMTGTGHGVEEVLTQATTLLSGLSRCAGLVVAAKQDTVFK"
BASE COUNT          428 a          800 c          786 g          448 t
ORIGIN      
        1 atggtcgcga acggcgtttg cggctctatg gtgagttgat aaagcgagag gtgttcggta
       61 ccgaacgcca aggcttcgtc aagctcgctg cgccaagcct tcaggctctg gcccggccgc
      121 gcatagatca gatcgagcga aacgcgcttg aacgtctgct gcgccagcgc caatgccgct
      181 ttggcctcgc gtacgtcgtg caggcggccc aggaagtgca aatccgcgtc gttcagcgcc
      241 tgaatgccca aggacagccg gttgacgccg gccacggcgt aatcagcgaa gcgcgccgct
      301 tccgcgctcg cgggattggc ttccaacgtg atttccacat cgttggccga aggcaacaga
      361 ccagtcgccg catcgataat gcgcccgacc gaacgcgcgc tcatcaagga cggcgtgccg
      421 ccgccgaaaa agatcgtttg cacaaccgga agctccgcgc tctgcaattc ggcaacgtga
      481 gtcatttcgc gcaagatcgc ttgcgcccag ccttcctcgt cgatgctggc gcggacatgg
      541 gaattgaaat cgcaataagg gcacttggcc gcgcagaacg gccaatggac gtaaaggccg
      601 aagccgttca tggcgcgaga acggctcgcg cgaacttctc gaaggcgcgg gcgcgatggc
      661 tgatggcgtg tttctgcgcc ggatcgattt cgccaaagct ccgctccatg ccctcggcga
      721 cgaagatcgg atcgtagccg aagccgttgg tgccgcgcgg cgggaaaacg agttcgccgc
      781 gtacttcgcc ttcgaagatc tccgtttcgc cggatggcgc ggcgagcgcc agcacgcaaa
      841 cgaatttcgc ggaacgatcc ttcgcgcctt tcgtcttcaa ttcgcgttcg acgcgcgcca
      901 tggccagtgc gaaatccttt tcgggaccgg cccagcgcgc cgaatagatt ccgggcgcgc
      961 cgtccagcgc cgcaacgcac aatcccgaat cgtcggcgag cgccgcaagc ccgcttgctt
     1021 cagccgcggc gcgcgctttc agcagcgcgt tgccggcgaa ggttgcgccg gtctcttccg
     1081 gttcggcgag acccagttcc gccgctccgc gcaccgcaat ggcgaacggc gacaagagat
     1141 cggcgatctc ccgcaccttg ccgcgattgt ggctggcgac gagaagaacg gagccgcgct
     1201 caagcttcat tggccgagcg ccgccttttg cagcgcgact agctcggcga tgcctttgcg
     1261 cgcctggacg agcagttggg cgaattgttc gtcgctgaac ggagcgcctt ccgccgttcc
     1321 ctggatctcg accagaccgc ccgagccggt gaggacgaaa ttggcgtcgg tctcggcggc
     1381 ggaatcctcg gcataatcga ggtcgagcat cacttggccc ttgacgatgc cgcaggatat
     1441 ggccgcgaca tgatccttga tcgccggttt ggtgaccatc ccggtctttt tcatgaaggc
     1501 gatacagccg gccagcgcca cgaaacctcc ggtgacggag gcggtgcggg tgccgccgtc
     1561 ggcttgcaaa acgtcgcaat cgatcgtgat ctggcgttcg cccagcgcgg agaggtcgac
     1621 gaccgcccgc aaggaacgcc cgatcaaccg ctgaatctcc tgggtccggc cggactgctt
     1681 gccggtggcg gcttcgcggc gcatgcgttc gtgggtggag cgcggcagca tgccatattc
     1741 ggccgtcacc cagcccttgc ccgaaccgcg cagaaaggac gggatcgcct cctccaggct
     1801 ggcggtcacc agcacatggg tgtcgccgaa tttgaccagg caagacccct cggcatgctt
     1861 ggcaaagccc ggttccaggc tcaccttgcg caactggtcg agcgcacgct catggcgcat
     1921 agagtttccc ttgaagtttg aggtgtcggc ctttacctag cggctccggc cacccggatg
     1981 caaaccgggc ccagccgttt tgtggatttt acaactggtt caagggtttg ggtatggctc
     2041 cctccagggc gaataccgag tttctggccg accggcagcg cgaagtcttc cgccgcctgg
     2101 tggaggcatt cctggcgtcc ggcgagccgg tcggctcgcg caccttgtcc cagcttttgc
     2161 ccatcgcgct gtcgccggcc tcgatccgca acgtcatggc cgacctggag accatggggc
     2221 tgctcactgc gccgcatacc agcgccgggc gggttcccac cgaaaagggc ctcagactgt
     2281 tcgttgacgg cctcttgcag atcggcgagc tggcgccggc cgagcgcgcc tccatcgaag
     2341 cgcgcatgac cggaaccggc catggcgtgg aagaggtgct gacccaggcg acgacgcttc
     2401 tttcggggct ttcgcgctgc gcggggttgg tcgtcgccgc caagcaggac acggttttca
     2461 aa
//
LOCUS       DASWPU010000481         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_41442,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000481 DASWPU010000000
VERSION     DASWPU010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41442"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VGC27_10165"
     CDS             <1..242
                     /locus_tag="VGC27_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282972.1"
                     /translation="LTQAAFARREGLRYPTFAHWVQQSNKGVLAPAVRFAQVRGPQPA
                     LDAPPQRLEVQLPDGTTVRGGEVALLAELVRALRS"
     gene            246..590
                     /gene="tnpB"
                     /locus_tag="VGC27_10170"
     CDS             246..590
                     /gene="tnpB"
                     /locus_tag="VGC27_10170"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY0282973.1"
                     /translation="MLPFTSSTRIFLALEPVDMRKQFNGLWSAAVERLGEDPKGGALF
                     GFINKDRTRLKLLYWDGTGVWVLAKRLEQGRFSWPTEAGKPKLALTAEALALILSGID
                     LKAGTLKAWYER"
     gene            677..>2044
                     /locus_tag="VGC27_10175"
     CDS             677..>2044
                     /locus_tag="VGC27_10175"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEY0282974.1"
                     /translation="MSTAPAHADPAALQQENALLRGENKALRDQLAWLKQKVFGPGRS
                     ETLDRAQQLIALDAATAPAPERSVETITYRREKGPRAARPTPAETFAHLPVKETIVII
                     PDEVKAQPEAFEQIGEERTFEVDLIAPQLFKREIVRPKFRRKADRSQPPVLAPAPLRP
                     VVGGYASAGLLAWIIVAKFVDHLPLYRQEKMLTRWGARISRQSLGDWVALVADRLEPL
                     HKLLLQHLLARGYLQVDETPIRCQDPDHPGSTVQGYLWVADDPSGDTVFRWANSRRHA
                     ELEALLGNFLGLLQSDAYGAYPAFAAAHPGVEWVGCWAHARRGFFEALSDSPKAAGVV
                     LRVIGQLYACEKRWDEAGVTDERAALRQTHFARPLYWLKKLATGLRTKHLNQSKLGKA
                     CGYLLAHWEALVAHCRHSRTRLDNNLVENAIRPSKLGAKNWLFVGHPDAGPRLAILYS
                     LLITCQ"
BASE COUNT          334 a          745 c          652 g          313 t
ORIGIN      
        1 gtctgacgca ggcggcattc gcgcggcgcg agggtctgcg ctacccgacg ttcgcccact
       61 gggtgcagca gtccaacaag ggcgtgctcg cgccggcggt acgcttcgcg caggtgcgtg
      121 ggccacagcc ggcactcgac gcgccgcccc aacgactcga ggtgcaactg cccgacggca
      181 ccacggtgcg tggtggcgaa gtcgcgctgc tggcggagtt ggtccgcgcc ctgaggagct
      241 agccgatgct gccgtttacc tcctccactc gcatcttcct ggcgctggag ccggtggaca
      301 tgcgcaagca gttcaacggc ctgtggtcgg ccgccgtcga gcggctgggc gaagatccca
      361 agggcggtgc gctgtttggc ttcatcaaca aggaccgcac gcgcctgaag ttgttgtatt
      421 gggacggcac tggcgtgtgg gtgctggcca aacggctcga gcagggccgc ttcagctggc
      481 ccaccgaggc gggcaaaccc aagctcgcgc tcaccgccga ggccctcgcc ttgatcctga
      541 gcggcatcga tctgaaggcc ggcacgctga aggcgtggta cgagcgctaa ggccgccggc
      601 ggcgacgcaa aatccccgcg gaaaaaagtg cgcacggcac cgcgttggcg tgtcctttgc
      661 tacgatacaa gggccgatgt ccaccgcccc cgcccacgcc gatcccgccg cgctccagca
      721 ggagaacgcg ctgttgcgcg gcgagaacaa ggcgctgcgc gaccagctcg cctggctcaa
      781 acagaaggtc ttcggcccgg gccgcagcga gaccctcgac cgcgcccagc agctcatcgc
      841 cctggacgcc gccacggccc ccgcgcccga gcgttcggtg gagacgatca cctaccggcg
      901 cgagaagggc ccgcgcgctg cgcggcccac gccggccgag acttttgcgc acctgccggt
      961 gaaggaaacg atcgtcatca tccccgacga ggtgaaggcc cagcccgagg ccttcgagca
     1021 gatcggcgag gagcgcactt tcgaggtcga tctgatcgcc ccgcaactct tcaaacgcga
     1081 gatcgtgcgt ccgaagtttc gccgcaaggc cgaccgtagc cagccgcccg tgctcgcccc
     1141 ggcgccgctg cggccggtgg tcggcggtta cgcctccgcc ggcctgctgg cctggatcat
     1201 cgtggccaag ttcgtcgacc accttccgct ctatcgtcag gaaaaaatgc tcacacgctg
     1261 gggcgcgcgc atctcccgcc aaagcctcgg cgactgggtg gcgctggtcg ccgaccggct
     1321 cgaaccgctc cacaagttgc tgctgcaaca cctcctcgcc cgcggttacc tccaggtcga
     1381 cgagacgcca atccgctgtc aggaccccga tcatcccggc agcacggtgc agggttacct
     1441 ctgggtcgcc gacgatccca gtggcgacac cgtctttcgt tgggccaact cccgccggca
     1501 cgcggagctg gaggccttgt tgggcaactt cctgggcttg ctccagtccg acgcctacgg
     1561 cgcgtatcca gccttcgccg ccgcgcatcc cggcgtcgag tgggtcggct gctgggccca
     1621 cgcccgccgc ggcttcttcg aagcactgtc ggacagcccg aaggccgccg gtgtggtgct
     1681 gcgcgtcatc ggccaactct acgcctgcga gaaacgctgg gacgaagcgg gggtcaccga
     1741 cgagcgcgcc gcgttgcgcc agacccactt cgcccggccg ctgtactggt tgaagaaact
     1801 ggcgacaggc ctgcgcacca aacacctcaa ccaatccaag ctcggcaagg cctgcggcta
     1861 cctgctcgcg cactgggagg cgctggtcgc ccattgccgc cacagccgca cccggctcga
     1921 caacaacctc gtcgagaacg ccatccgtcc cagcaagctc ggagcgaaaa actggctgtt
     1981 cgtcggtcat cccgacgccg gccctcgcct cgccatcctc tactcgctgc tcatcacctg
     2041 ccag
//
LOCUS       DASWPU010000482         5625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_96170,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000482 DASWPU010000000
VERSION     DASWPU010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96170"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..518
                     /locus_tag="VGC27_10180"
     CDS             129..518
                     /locus_tag="VGC27_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282975.1"
                     /translation="MPLTPNLPVGVEPTLDELRAGAPEVIDETWKDDIVNRLLLQLKR
                     QLCQLENTKPTTEIDQANIRATNVKTLASIERTLDRLLQMEQQRALARETKVAAKNDG
                     ALAKLERRINLLIAASNPQESVAAPEK"
     gene            472..1716
                     /locus_tag="VGC27_10185"
     CDS             472..1716
                     /locus_tag="VGC27_10185"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEY0282976.1"
                     /translation="MPPQTLKNLWRRLKNDDAALLNETWSFWARPEQLPPQGPWRIWL
                     FLGGRGAGKTRAGAEWIADGVRRGTMRRIGLVGATFADARSVMIEGESGLLSVSPSAS
                     FEPSNRRVLWPTGAVATVVSAEEPDGVRGHQFDAVWADEFCKWAEPQAALDMVLMALR
                     LGEDPRMAVTTTPRNIKALRELMAACDVAVTRSNTRANAANLAPTFLSSLELRFGGTR
                     LGRQELDAELIEDNDAALWRRDWIERARVRTAPPLTRVVVAVDPPASIAGDECGIVVA
                     GLAENGAAYVIADCSVGGVSPASWAARVADAYEQFEADGIVAEANQGGDMVKQVLLDA
                     LPNVSVKLVHATRDKRTRATPAAALYEQGRVHHVGAFPELEDQMCSYDGEGKSPDRMD
                     ALVWALADLFPRTRATPKVRTV"
     gene            1857..3023
                     /locus_tag="VGC27_10190"
     CDS             1857..3023
                     /locus_tag="VGC27_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEY0282977.1"
                     /translation="MLSHFFRRAPETKSAPLIALSLMGAPRWGGRDFPSLAREAVMGN
                     AVAYRCVRMIAEGAASVPWLLYDGAQEIESHPLLSLLAGPNPQDEGTALFERWYAYLQ
                     CAGDSYLEAVSAGGEVRELYVLRPDRVSVVADARGWPIAYDYRVGGFTARIARDPSTG
                     FMPMLHSTLFHPLDDHYGLSPVQAAANAVEVHNAGAAWTKALLDNAARPSGALIYKGP
                     EGAPSLSDDQFQRLKRELEEAYQGASNAGRPMVLEGGLDWRSMSYSPADLDFGRTRDV
                     AAREIALAFGVPPMLLGIPGDNTYANYREANLVFWRQTVLPLVARTAKALTRWLAPRF
                     GETLRLGYDSDAVDALAIERESIWDRLSNAKFLTLNERRQAAGYSPVEGGDVMS"
     gene            3020..3280
                     /locus_tag="VGC27_10195"
     CDS             3020..3280
                     /locus_tag="VGC27_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282978.1"
                     /translation="MTLTEYVQPMLDKRLPAALMAAFLLQTAGALFWAGSAAERISTL
                     ERTLAADQGAIARVAVLEDQIGTIKQTLDRIETKLDRTNLNH"
     gene            3419..3949
                     /locus_tag="VGC27_10200"
     CDS             3419..3949
                     /locus_tag="VGC27_10200"
                     /inference="COORDINATES: protein motif:HMM:NF016471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEY0282979.1"
                     /translation="MTTIHYARRRLARLAAPARLSELADDEFEGYASLFGVPDGAGDV
                     VARGAFAASLRKRPPERVRMLYQHFAHEPLGVWQTIREDARGLYVRGRLVLDVTRARE
                     VRSLLRDGALNGLSIGFRTVRATRGAGHRLLNEIELWEISVVTFPLLAESKVTAIGAK
                     GDLARTLRQASAALRA"
     gene            3961..5211
                     /locus_tag="VGC27_10205"
     CDS             3961..5211
                     /locus_tag="VGC27_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEY0282980.1"
                     /translation="MELESKAVGSPDIKDAFDEFLRAFEAFKSANNERLEQLETRSAD
                     VVTEEKVDRINRALDDQKRVLDELTLAAARPAVAGGPGAAGERKAAPAARETKAAFER
                     YIRKGDAGGLDALELKAMSAGSPADGGYTVPLEIEQTIDRVLAKVSPLRAIATVRQIG
                     GNVYRKPIAKTGAATGWVAETGARAQTDAPTLAAIDFPAMELYAMPAATQTLLDDSQV
                     DLEAWLAGEVQTVFAEQEGAAFVSGNGTTQPKGFLTETKVAEASWEWAKLGFVLSGGD
                     GAFAAADPTDVLINLAYAPKQAYRANGSWVMNRKTESVIRKFKDSQHNYIWQPGASAG
                     QQPTLLGYPVAEAEDMPDIASGSYSIAFGDFARGYLIVDRVGVRVLRDPYSAKPYVLF
                     YTTKRVGGGVQNFEAIKLMKFATS"
     gene            5367..>5625
                     /locus_tag="VGC27_10210"
     CDS             5367..>5625
                     /locus_tag="VGC27_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HEY0282981.1"
                     /translation="MSLTLVTPPAAEPVTLAEAKAHLKVDTSADDALITSLIVAARAR
                     AEWHSGCAFVTQSWTLWLDAWPRNGLVEIPLPPLQSVTQVRV"
BASE COUNT          984 a         1909 c         1823 g          909 t
ORIGIN      
        1 gggctgtaat tgcgcggtcc ggcggccgcc ccgagtcgcc ggtggtagaa gtcaaaaaaa
       61 tttccagtcc ccccttgaac cgacaaggtc gaattaccaa ataccccata tcccgataaa
      121 ttcatccgat gcccctgacc ccgaacctcc ccgtcggcgt cgagccgacg ctcgacgagt
      181 tgcgggcggg cgcacccgaa gtcatcgatg agacatggaa ggacgacatc gtgaaccggt
      241 tgcttctcca gctgaaacgg cagctttgcc aattggaaaa caccaagccc acgaccgaaa
      301 tcgaccaggc gaacatccgc gccacgaacg tgaagacgct cgcctccatc gaacgcacgc
      361 tcgaccggct gctgcagatg gaacaacagc gcgcgctggc gcgcgaaacc aaggtggcgg
      421 cgaagaatga cggagccctt gccaagcttg agcgcaggat caatctgctc attgccgcct
      481 caaaccctca agaatctgtg gcggcgcctg aaaaatgacg atgcagcgct cctcaacgaa
      541 acctggagtt tctgggcgcg gcccgagcaa ttgccgccgc agggtccatg gcgcatctgg
      601 ctgtttctcg gcgggcgcgg cgcgggcaaa acccgcgccg gagcggaatg gatcgccgat
      661 ggcgtgcgcc gcggcacgat gcgccgcatc ggcctcgtcg gcgccacctt cgccgatgcg
      721 cgcagcgtga tgatcgaagg cgaatccggc ttgctgtcgg tgtctccctc cgcgtcgttc
      781 gagccttcga accggcgcgt gctgtggccc accggtgcgg tggccaccgt ggtgtctgcc
      841 gaagagcccg acggcgtgcg cggccaccag ttcgatgcgg tgtgggccga cgaattctgc
      901 aaatgggcgg aaccgcaagc cgcgctcgac atggtgctga tggcgctgcg cttgggcgag
      961 gacccgcgca tggcggtcac caccacgccg cgcaacatca aggctttgcg cgagctgatg
     1021 gcggcctgcg atgtggcggt gacgcgctcg aatacgcgcg ccaacgcggc caatctggcg
     1081 ccgacttttc tttcgagcct ggaactgcgc ttcggcggca caaggctcgg ccgtcaggaa
     1141 ctggacgccg aactgatcga agacaacgac gccgcgctgt ggcggcgcga ctggatcgag
     1201 cgtgcccgcg tccgcacagc gccgccattg acccgcgtgg tggtggcggt cgatccgccc
     1261 gcctccatcg cgggcgacga atgcggcatc gtggtcgcgg gcctcgcgga aaacggggcg
     1321 gcttacgtga ttgccgattg ttcggtgggc ggggtctcgc ccgcaagctg ggccgcgcgc
     1381 gtcgccgacg cttacgaaca gttcgaggcc gacggcatcg tcgccgaagc caatcagggc
     1441 ggcgacatgg tgaaacaagt gctgctcgat gcgctgccca acgtctccgt gaagctggtg
     1501 catgccacgc gcgacaagcg cacccgcgcc acgcccgccg ccgcgcttta cgaacaaggc
     1561 cgggtgcatc atgtcggcgc gtttccggag cttgaagacc agatgtgctc ctatgacggc
     1621 gaaggcaaaa gccccgaccg catggacgcg ctggtctggg ccttggccga cctcttcccg
     1681 cgcacccgcg ccacgccgaa agtacgaact gtgtaacgca atcctcattg tcatccccgg
     1741 cgagccgcgc tcttcacgcg gcgagggaag gggatccaga aaccgcgcaa gcgcatggat
     1801 tcccttcccc tcgctacgct cggccgggaa tgacaatctt tcttgagaaa actcccatgc
     1861 tctctcattt cttccgccgc gctcctgaaa ccaagagcgc gccgctgatc gccctctcgc
     1921 tgatgggggc gccgcgctgg ggcgggcgcg attttccctc gctcgcccgc gaggcggtga
     1981 tgggcaacgc cgtcgcctat cgctgcgtgc gcatgatcgc cgaaggcgcg gcctcggtgc
     2041 cgtggctcct ctatgacggt gcgcaggaga tcgaatccca cccgctgctc tccctcctcg
     2101 ccgggcccaa tccacaggac gaaggcaccg ctctgttcga gcgctggtat gcctacctcc
     2161 aatgcgccgg cgattcctat ctcgaagcgg tgagcgcggg cggcgaggtg cgcgagcttt
     2221 acgtgctgcg gccggatcgc gtcagcgtcg tggccgacgc gcgcggctgg ccgatcgcct
     2281 acgattacag ggtggggggc ttcaccgcgc gcatcgcccg cgacccctcc acgggcttca
     2341 tgccgatgct gcactccacc ctgttccatc cgctcgacga tcattacggc ctgtcgccgg
     2401 tccaggcggc ggcgaacgcg gtggaggtcc acaatgcggg cgcggcctgg accaaggcgc
     2461 tgctcgacaa tgcggcgcgg ccgtcggggg cgctgatcta caaggggccg gagggcgcgc
     2521 cttcgctcag cgacgaccag ttccagcgcc tcaagcgcga gctggaagag gcttaccagg
     2581 gcgcctcgaa cgccggccgg ccgatggtgc tggaaggcgg cctcgactgg cgctcgatga
     2641 gttattcgcc cgccgacctc gattttggcc gcacccgcga cgtcgccgcg cgcgagatcg
     2701 cgctcgcctt cggggtgccg ccgatgctgc tcggcattcc cggcgacaac acctatgcga
     2761 actaccgcga ggccaatctg gtgttctggc gccagacggt tcttcccctc gtcgcgcgca
     2821 cggccaaggc gctgacccgc tggctggcgc cgcgcttcgg cgagacgctg cgcctcggct
     2881 acgattccga cgccgtcgat gcgctcgcca tcgaacgcga aagcatctgg gatcggctct
     2941 cgaacgcgaa gttcctcacc ctcaacgaac gccgccaagc cgcgggctac tcgccggtgg
     3001 aaggcgggga tgtgatgtcg tgaccctcac cgaatatgtc cagccgatgc tggacaagcg
     3061 ccttccggcc gccctcatgg cggccttttt gttgcagacg gcgggcgcgc tgttctgggc
     3121 gggttcggcg gcggagcgca tctccactct cgagcgcacg ctcgccgccg accagggggc
     3181 catcgcccgc gtcgccgtgc tggaagacca gatcggaacg atcaagcaaa cgctcgaccg
     3241 catcgagacg aaattggatc ggacgaactt gaaccactga taagtttctg ctcccccctt
     3301 gagggggagc caaattcgcg aagcgaattt ggtggggggt ctacgcgaag tacccctccc
     3361 cgaaatctgc tttgcagatt tcgaccctcc ctcaagggga gggtgaagaa agcgaagaat
     3421 gaccaccatc cactatgcga ggcggcgtct cgcacgcctt gccgcgcccg cgcgcctcag
     3481 tgaactcgcc gacgacgagt tcgaaggcta tgcctcgctg ttcggcgtcc ccgacggcgc
     3541 gggcgacgtg gtggcgcgcg gcgcctttgc ggcgtcgctg cgcaagcgtc cgcccgagcg
     3601 cgtgcgcatg ctgtatcagc atttcgcgca cgagccgctc ggcgtctggc aaaccatccg
     3661 cgaggatgcg cgcgggcttt acgtgcgcgg ccgcctggtc ctcgacgtga cgcgcgcccg
     3721 cgaggtgcgt tcgctgctgc gcgacggcgc gctcaacggg ctttccatcg gctttcgcac
     3781 cgtgcgcgcc acgcgcggcg cgggccatcg cctgctcaat gaaatcgagc tttgggaaat
     3841 ttcggtcgtg accttcccgt tgctcgcgga atcgaaggtc acggccatcg gcgcgaaggg
     3901 cgacctcgct cgcacgctgc gccaggcaag cgcggctctg cgcgcatgaa ggagagaagt
     3961 atggaacttg aatccaaagc cgtcggatcc cccgacatca aggacgcgtt cgacgaattc
     4021 ctgcgcgcct tcgaggcgtt caagagcgcc aataacgagc gcctggaaca gctcgaaacc
     4081 cgctcggccg atgtcgtcac cgaggaaaag gtggaccgca tcaaccgcgc gctcgacgat
     4141 cagaagcgcg tgctcgacga gctgaccctg gccgccgccc gtcccgccgt cgcgggcggc
     4201 cccggcgcag cgggagagcg caaagccgcg cccgccgcgc gcgaaaccaa ggccgccttc
     4261 gagcgctaca tccgcaaggg cgacgcgggc gggcttgacg cgctggagct gaaggcgatg
     4321 tcggccggtt cgcccgccga cggcggctac accgtgccgc tggagatcga acagaccatc
     4381 gaccgggtgc tggcgaaggt ttcgccgctg cgcgccatcg ccacggtccg ccagatcggc
     4441 ggcaacgtct atcgcaagcc catcgccaaa acgggcgcgg ccaccggctg ggtcgccgaa
     4501 accggcgcgc gggcgcagac cgatgcgccg accctggccg ccatcgattt tccggcgatg
     4561 gagctttacg ccatgccggc cgcgacccag accctgctcg acgacagcca ggtcgatctc
     4621 gaagcctggc tggcgggcga agtgcagacc gtgttcgccg aacaggaagg cgccgccttc
     4681 gtttccggca acggcaccac ccagcccaag ggcttcctca ccgaaaccaa agtcgccgaa
     4741 gcgtcgtggg aatgggccaa gctcggcttc gtcctgtcgg ggggcgacgg cgccttcgcc
     4801 gccgccgatc ccaccgatgt gttgatcaat ctcgcctatg cgcccaagca ggcttaccgc
     4861 gccaacggtt cgtgggtgat gaaccgcaag accgaaagcg tcatccgcaa attcaaggac
     4921 agccagcaca attacatctg gcagcccggc gccagcgcgg gccagcaacc gacgctgctc
     4981 ggctatccgg tggcggaagc cgaggacatg cccgacatcg cttcgggctc ctactccatc
     5041 gccttcggcg atttcgcccg cggctatctg atcgtggatc gcgtcggggt gcgggtgctg
     5101 cgcgatccct attcggccaa gccttacgtg ctgttctaca ccaccaagcg tgtcggcggc
     5161 ggggtgcaga acttcgaagc gatcaagctg atgaagttcg cgacatcgtg agacacagca
     5221 cttcaccctc cccttgaggg agggtcgaaa tctgacgcga gcgaagcgag aagaagattt
     5281 cggggagggg tgatgcttca cgaatacccc tccccgaagc acttcgtgct tcgaccctcc
     5341 ctcaagggga gggtgaaaga gaagacatgt ctcttacttt ggtaacccct cccgccgccg
     5401 agccggtgac gctcgccgag gcgaaggcgc atctcaaggt ggatacgtcc gccgacgatg
     5461 cgctgattac ctcgctgatc gtcgccgcgc gggcgcgcgc ggaatggcac agcggctgcg
     5521 ccttcgtgac ccaaagctgg acgctgtggc tcgatgcctg gccgcgaaac ggcctggtcg
     5581 agattccgct gccgcccttg cagagcgtga cgcaggtgag ggtcc
//
LOCUS       DASWPU010000483         8299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_28310,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000483 DASWPU010000000
VERSION     DASWPU010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28310"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..275)
                     /locus_tag="VGC27_10215"
     CDS             complement(108..275)
                     /locus_tag="VGC27_10215"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY0282982.1"
                     /translation="MNKNRIVGSGKQIKGTIKEAVGKVVGNTKLQVEGKADKVEGKIQ
                     NTAGSIEDALK"
     gene            complement(346..501)
                     /locus_tag="VGC27_10220"
     CDS             complement(346..501)
                     /locus_tag="VGC27_10220"
                     /inference="COORDINATES: protein motif:HMM:NF019696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEY0282983.1"
                     /translation="MCKRSTLLAFVLLVLSGAVLALGACHTIAGAGEDVSHAGQAIEK
                     SAEKHAP"
     gene            complement(603..758)
                     /locus_tag="VGC27_10225"
     CDS             complement(603..758)
                     /locus_tag="VGC27_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEY0282984.1"
                     /translation="MLGTILLIVLVLFLVGALPNWKHSTNWGYGPSGGLGIIIVIVLV
                     LVLMGRI"
     gene            complement(911..1111)
                     /locus_tag="VGC27_10230"
     CDS             complement(911..1111)
                     /locus_tag="VGC27_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282985.1"
                     /translation="MQFVQADHAKDSRLRFVFNHSVMSFSLASDATFGEIALTLDDIS
                     NRRHGNPLAIDVRLKQRDAEFR"
     gene            complement(1198..5646)
                     /locus_tag="VGC27_10235"
     CDS             complement(1198..5646)
                     /locus_tag="VGC27_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY0282986.1"
                     /translation="MPKGRPLEVKKVLPGSSGRPLPSRGPPRGPVDFPVVGIGASAGG
                     LEACKLFVGALPAHSGMAFVLIQHLDPTHESMMVGLLAGHTPMAVLQAVDGMAIEREH
                     LYVIPPGTYLSVVDGALHLSQPQAPHGARLPFDFLLQSLAEECGPRAVCVILSGTGSD
                     GSLGLKAVKEKGGLVIAQDTEEAGYDGMPRNAIATGAVDLVLPAAKIPEALVKFDRRM
                     TLTRKKNGSAPQDKIQDWLPEIVALLRTKTGHNFTLYKPGTLQRRVERRMALAAIESD
                     DMNRYLDILRGDTGELELLAKDLLINVTSFFRDPKVFDILAEKAIPELLRSQQPDHPV
                     RIWVPGCSTGEETYSLAMLFREQISAAKRDIKLQVFASDVDTDAVASAREGLYPDTIE
                     ADVSAERLARFFSKEDHGYRISPDLRATVVFTVQDVLADPPFSRLDLVSCRNLLIYLR
                     QEAQAKVLSLFHFALREGGILLLGSSETVGGADNRFETISKAGQIYRHTARSRPGELG
                     FAMGGNDSVHIPLRPGQGQVPTRQAAFADLCRRLVMETYAPAAVLINRKYECLYTLGP
                     TDRYLRVAPGHPTHDLLAMVRQDMRTKLRSAVQQASQENGRVAVSGGRTNRDGDTVSF
                     SIEVQPILSDGEELLLICFIDEPKQEQKQGRTPAPIDVTRVAELEQELEATKTELHGA
                     IRNLEISNEEQAAINEEALSVNEEFQSTNEELLASKEELQSLNEELTALNSQLQETLE
                     LQRTASNDLQNVLYSTDVATLFLDTNLNIRFFTPATKSLFSVIPGDIGRPLADLNSLA
                     ADGVLLTDARAVLHTLAPVEREIEAQNGAWYIRRVMPYRTQDNTVEGVVITFSDSTER
                     RRAADALEAAKRQAELATLAKSRFLAAASHDLRQPLQTLALLQGQLAKTVEGEKAQRL
                     VARIDETLGAISGMLNALLDINQIEAGIVHAETVGFPINGLLDRLRDEFSYHAQAQGL
                     SLRVVPCGLSIQSDPRLLELMIRNLLSNALKYTKSGKVLLGCRRHKETLSIEVLDTGV
                     GIPKVEFQRIFEEYHQLDNASRERSRGLGLGLSIVRRLGDLLGHRITVGSNMGRGSAF
                     AIEVALQPAGTALEQAWRGKDDGIAEPPRRSGAILIVEDDPEVRGLLEILLKDEGHRT
                     VTAPDGVAALEMATVGTPPPDLILADYNLPNGMNGLQLAAKLREKLHRRIPIIILTGD
                     ISTGTLRDIAHQDCVQLNKPVRVKELTQAIRRLLAASQPPAQARAPHAAEAAGTARPP
                     VIFIVDDDARIREGIRGMFEEEGMAVEDYGTCEAFLDAYHPGREGCLLIDAYMPGMSG
                     LELLKHLRDAGHRLPAIMITGNSDVPMAVQAMKAGASDFLEKPIRNSELFASVERALE
                     QSRDASKLSEWRETAADHIAKLTPRERQVMELVLAGQPSKNIAADLGISQRTVENHRA
                     SIMEKTDTKSLPALARLALAAAQNGTGELLIQPDSEASEARRTTGK"
     gene            6156..6410
                     /locus_tag="VGC27_10240"
     CDS             6156..6410
                     /locus_tag="VGC27_10240"
                     /inference="COORDINATES: protein motif:HMM:NF018350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEY0282987.1"
                     /translation="MTAASRFPPAPKRWVAHRKAEVVAAVRGGYLSLDEACKRYALSL
                     EEYLSWQREIDRFGLAGLRVYRPHQRRREKLRDDEKEQGS"
     gene            6461..6742
                     /locus_tag="VGC27_10245"
     CDS             6461..6742
                     /locus_tag="VGC27_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282988.1"
                     /translation="MTEFKLPLLPRDRGWLELRERLARAARDLHFSAVANENGNGPAL
                     LMIAGNLAGLALSIPLRPNPISSGGLVDASTLYATQILQRDTTDDGEDK"
     gene            6851..>8299
                     /locus_tag="VGC27_10250"
     CDS             6851..>8299
                     /locus_tag="VGC27_10250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0282989.1"
                     /translation="MIDSSKIYGCRILIVGDRPANVRLLEKMLRRAGYTCIASSADPT
                     AVCELHLKNRYDLILLDLQERRADGFLVLEALKKIEPEDYLSVLAIAAQSDHKQRALQ
                     AGAKDFISNPLDRSEVLTRIYNMLEVRLLHGETKAHGKLLEEKVRERTAELRRSEEMF
                     RELAANIPEALWIRDIEGQTLRYVNPAWQELSGLSAAPGDPIDKLYQTVHPDDRQRIA
                     NERRRAPNTHASSEYRLVRPDRSVRWVHARTFPIANPSGKSPWLVEILEDVTQRREVQ
                     RQLVHLARHDALTGLPNRMFLYESLQEALVHASEDHLVVSVLLLDIDYFKNVNDTLGH
                     AVGDAVLREFAARLAQCVRPGDIVGRLAGDEFAVIVLTPADSNGAIDVANRIRDSLQN
                     PLVLQDKNILVTASVGIASYPTDTSDLEVLIRYADAALYDAKAAGRNGFRRYTAELNA
                     RALERADTEGALRLAQGREEFLLYYQPKMQIDN"
BASE COUNT         1633 a         2507 c         2523 g         1636 t
ORIGIN      
        1 ccgtgcgcag gcttggatga acggtcctgc gcgccctgaa ggggcgcccg aggaactgca
       61 tcatgaccgt tactggcccg atttggagaa atgcctcggg cgtttcgcta tttcagggcg
      121 tcttcgatcg atccagccgt attctggatc tttccctcga ctttgtcggc cttgccctcg
      181 acttgcagct tggtgttgcc gacaactttg ccgacggcct ctttgatcgt gcctttgatc
      241 tgtttgccag atccgacgat gcggttctta ttcatgactc tttacctcaa tacacggtgt
      301 gggcgcggat tgcgcaacct ggtgaatgct cgttcatgat gcggatcagg gtgcgtgctt
      361 ttccgcactc ttctcgatgg cttgacccgc gtgggatacg tcctcgccgg ctcccgcgat
      421 ggtgtgacag gcaccgaggg ccagcacggc gcccgagagc acgagcagga caaaggctaa
      481 cagagtcgat cttttacaca tggcgattac cttcaaatct ggtttgtgcc tgtcccgcgc
      541 cgggatcccg gcggtcaaca cccgccagga cgcgaacgcc cgccgccggc gccggcgccg
      601 gactaaatcc gccccatcag caccaagaca agaacgatga cgatgatgat gcctagtcca
      661 ccgctcgggc cgtagcccca gttcgtgctg tgcttccagt ttggcaaggc gccgaccaaa
      721 aacagcacaa ggacaatcaa cagaatggta ccgagcatgg gtcgaactcc taagaagatt
      781 accgtgaaac gccgggttaa gccgttttca ctttgggcac ttctacggcc aagaaagcct
      841 gatcggctat agcgtagcgg gcggcgagag ggtggcggta gcctcggaaa ctactcagac
      901 gcgtcacgga tcatctgaat tcggcgtcgc gctgcttcag tctgacgtcg atggcgagcg
      961 gattgccatg gcggcgattc gaaatatcgt ccaaggtcag tgcgatctcg ccgaaggtcg
     1021 catcggaagc aaggctgaac gacatgaccg aatggttgaa gacaaatctt aagcggctgt
     1081 ctttcgcatg atcggcttga acgaactgca tgggactctc cgcggtcttg ccgtcagcag
     1141 gacaatcaat tgcggaggat tgtcggtacc ccgatagaga gtcgatagga tcggaatcta
     1201 cttaccggtt gtccgccgcg cttcggaagc ctcggagtcg ggctgaatga gcaattcgcc
     1261 cgtgccgttc tgggcggcgg caagcgccag tcgggccagc gccggaagag acttcgtgtc
     1321 ggtcttttcc atgatcgagg cgcgatggtt ctcgaccgta cgctggctga tgccgaggtc
     1381 ggcggcgatg ttcttgctcg gctggccggc aagaaccaac tccataacct ggcgctctcg
     1441 cggcgtgagc ttggcaatat ggtccgccgc cgtctcccgc cattcggata gtttgctcgc
     1501 atcccgcgac tgttcgagcg cgcgctcgac gctggcgaac agttcgctgt tgcggatcgg
     1561 cttttcgaga aagtccgagg cgcccgcttt catggcttga accgccatcg gcacgtcgct
     1621 gttgccggtg atcatgatgg ccggcagccg atggcccgca tcgcggagat gcttgagcaa
     1681 ttcgagcccg ctcattccgg gcatgtaggc atcgatcaac aggcatcctt cgcggcccgg
     1741 atgataggca tcgagaaacg cttcgcaggt cccgtaatcc tcgacggcca taccctcttc
     1801 ctcgaacatg ccgcggattc cctcgcggat gcgggcgtcg tcgtcaacga tgaagatgac
     1861 cggcggccgc gccgtgccgg ccgcttcggc ggcgtgcgga gcgcgagcct gcgccggcgg
     1921 ctgcgacgcc gcgagaagac gccggatcgc ttgcgtcagt tccttcaccc ttaccggctt
     1981 gttgagctgc acgcagtcct ggtgcgcgat gtcgcgcaag gtgccggtcg atatgtcgcc
     2041 ggtcagaatg atgatcggaa tccggcgatg aagtttttcc cgcagcttcg cggcaagctg
     2101 aagcccgttc atgccgttcg gcaggttgta gtccgccagg atgaggtccg gagggggcgt
     2161 ccccaccgtc gccatttcca gcgctgcgac gccatcgggc gccgtcaccg tgcgatggcc
     2221 ttcgtccttg agaaggattt cgagcagtcc gcgcacttcg ggatcgtctt cgacgatcaa
     2281 gatcgcgccc gagcggcggg ggggttcggc gatcccgtcg tcctttccgc gccaagcctg
     2341 ctcaagcgcc gttccggccg gctgcagcgc gacctcgatg gcaaaggccg agcctctgcc
     2401 catgtttgag ccgacggtta tccgatggcc cagcaaatcc cccaggcgcc gcacgataga
     2461 gagaccgagg ccgagcccgc ggctgcgctc gcgcgaggcg ttgtcgagct ggtggtactc
     2521 ctcgaagatt ctctgaaatt ccactttggg gatgccgacg cccgtatcca gaacttcgat
     2581 gctcagcgtt tccttatgcc ggcggcagcc aagcagtacc ttgccgctct tggtgtattt
     2641 caaggcgttc gacagcaggt tgcggatcat caattcgagc aggcgcggat cgctttggat
     2701 cgaaagaccg cacgggacca cgcgcaaaga cagcccttga gcttgcgcgt gatagctgaa
     2761 ctcgtccctc agccggtcga gcaggccgtt gatcggaaac ccgacggtct cggcgtggac
     2821 gatcccggcc tcgatctggt ttatgtcgag cagcgcgttc agcatgcccg atatggcgcc
     2881 caacgtctcg tcgatacgcg cgaccagtcg ctgcgccttc tccccttcga cggtcttcgc
     2941 caactgtccc tgaagcaggg caagcgtttg cagcggctgg cgcaggtcgt gactcgcggc
     3001 ggcgagaaag cgcgattttg ccagcgtcgc cagctccgcc tgccgcttgg ccgcctccag
     3061 cgcgtccgcc gcgcgcctgc gctcggtgct gtctgaaaag gtgatgacga cgccctcgac
     3121 cgtattgtcc tgggtgcgat agggcatgac gcggcggatg taccaagcac cgttctgcgc
     3181 ttcgatctcc cgctcgaccg gggctagggt atgcaacacc gctcttgcgt cggtcagaag
     3241 aacgccgtcg gcggccagcg aattgaggtc ggcgagcggc cgcccgatgt cgccgggaat
     3301 gacgctgaag agcgacttgg tggccggcgt gaagaagcgg atgttgaggt ttgtgtcgag
     3361 gaacagcgtc gcgacatcgg tgctgtagag gacgttctgc aggtcgttgg aggccgtgcg
     3421 ctgcagctcc agggtttcct ggagctggct gttgagggcg gtaagctcct cgttgagcga
     3481 ctgcaattct tccttggagg cgagcaattc ctcgttggtc gattggaatt cctcgttgac
     3541 ggacagcgcc tcttcgttga tcgccgcctg ttcttcgttc gatatctcga ggttgcggat
     3601 ggcaccgtga agctccgtct tcgtggcttc gagttcctgc tcaagctcgg cgacacgcgt
     3661 aacgtctatg ggagccggcg tgcggccttg cttctgctcc tgcttgggct cgtcgataaa
     3721 gcagatcaac agcaattcct cgccgtcgct caggatcggc tgcacttcga tgctgaacga
     3781 aaccgtatcg ccgtcgcggt tggtccggcc gccggaaacg gcaactcgcc cattctcttg
     3841 actggcctgc tggacggcgg accggagctt ggtgcgcatg tcctggcgca ccatggcgag
     3901 caaatcgtgg gttgggtgcc ccggcgccac gcgcagataa cggtccgtcg gccccagggt
     3961 atagagacat tcgtacttgc ggttgatcaa gaccgccgcc ggcgcataag tttccatcac
     4021 cagccgccgg caaagatcgg cgaatgcggc ttggcgcgtg ggtacttgac cttgcccggg
     4081 acgcagaggg atgtgtacgc tgtcgttgcc gcccatcgca aagccgagtt ctccgggccg
     4141 gctgcgggcg gtgtgccgat agatctgccc ggccttggag atcgtctcaa agcggttgtc
     4201 ggcgccgccg accgtttccg aactgccgag aagcagaatg ccgccttcgc gcagcgcgaa
     4261 atggaagagc gagaggacct tcgcctgcgc ttcctggcgc agatagatca ggagattccg
     4321 gcatgaaacc aggtcgagac gcgagaacgg gggatcggcg agcacatcct gtacggtgaa
     4381 gaccaccgtg gcgcgcagat cgggcgatat tctgtaaccg tgatcttcct tcgaaaagaa
     4441 gcgggcaagc cgttccgccg acacgtccgc ctcgatcgta tcggggtaga gtccctcccg
     4501 cgcgctggcc acggcatccg tgtcgacatc ggaagcgaag acctgaagct tgatatcgcg
     4561 cttcgccgcc gaaatctgct cgcggaagag catggcaagg gaataggttt cctcgcccgt
     4621 gctgcacccc ggcacccaga tgcggaccgg gtgatcgggc tgctggctgc ggagcagctc
     4681 gggaatagcc ttttccgcga ggatgtcgaa caccttcgga tcgcggaaga agctggtgac
     4741 gttgatcagc aggtccttgg ccagaagttc cagctcgccg gtgtcgccgc gcaggatgtc
     4801 gagatagcga ttcatgtcgt cggactcgat cgccgccagc gccatgcgcc gctcgacccg
     4861 gcgctgcagg gtgccgggct tgtaaagcgt gaagttgtga ccggtcttcg tgcgcagaag
     4921 cgcgacaatc tcgggcagcc aatcctgtat tttatcctgc ggcgccgaac cgttcttctt
     4981 tcgggtcagc gtcatccgcc ggtcgaattt gacgagcgcc tccgggatct tcgccgccgg
     5041 cagcacaagg tccaccgcgc ccgtcgcgat agcgttccgc ggcatgccgt cgtagccggc
     5101 ctcttcagta tcctgggcga tgaccaaccc gcccttttcc ttcacggcct tcagcccgag
     5161 gctgccgtcg ctcccggttc ccgaaaggat cacgcagacg gcgcgcggac cgcactcctc
     5221 cgccagcgac tgcaacagaa aatcgaaagg caggcgcgcg ccgtgaggcg cctgcggttg
     5281 cgagaggtgc aaagctccgt caacgacgga gagataggtt cccggcggaa tgacatagag
     5341 atgctcgcgc tcaatcgcca ttccatctac ggcttgcaga acggccatcg gcgtgtgacc
     5401 cgccagcagg cctaccatca tgctttcgtg cgtcggatcg agatgctgga tcagaacgaa
     5461 ggccatgccg ctgtgggcgg gcagcgcgcc cacaaacagc ttgcaggctt caagaccgcc
     5521 tgccgaggcg cctatcccga caaccggaaa atccaccggg ccgcggggag gaccgcgtga
     5581 cggcagcggc cggccgctcg atcccggcag cacctttttt acctctagcg gtcggccttt
     5641 tggcattctt cttttgttct cactaattct tttcccaaca gaaagtgcgc cccgccttct
     5701 gatgggaatt tgtagaatca acactatcac accccccagc cgcggcggca agcctgggca
     5761 aagaaacaaa gcgacaaatg ccagtctttt cccggatggc ggatgcaaaa aatcgccgca
     5821 atcatttatt ggtgaacatt cgtaaatgtg catttaaaaa acgttaggta aacagaaata
     5881 taaaatagga caaccagcaa atatacatca tttctgcaaa ataaaagatg agaggcgatg
     5941 gcttgcgtat attgataaat ttaaattagt caatcctctg agtactttcc gatactatca
     6001 attgcgattc ccccgcataa aattcacttc aatctgcgga gtgggccatg cggaattccc
     6061 aacaagcatt gacggctgaa aatacagaga aagacgatgc gtccattggc catgatgcaa
     6121 atcccaatga acgtcccggc accagtaaca aagagatgac ggcggcctcc cgctttcccc
     6181 ctgcgcccaa gcgatgggtg gctcatcgga aagcggaagt cgtggcggct gtgcgcggcg
     6241 gatatctcag tctcgacgaa gcgtgcaaac gctatgcgct gtcgctggaa gagtatctct
     6301 cttggcagcg cgaaatagac cgcttcggcc tggcgggatt gcgcgtctac agaccgcatc
     6361 agagacggcg cgagaagctt agggacgatg agaaggaaca agggtcgtaa ctcggtgatt
     6421 tgtgccgcgt cgggcgggag acaggtaatg gtggcgtttc atgacggaat tcaaactgcc
     6481 tctactgccg cgggatcgcg gctggcttga gcttcgcgaa cggcttgccc gtgcggcgcg
     6541 tgacctccat ttctccgcgg tagcaaacga aaacgggaac ggacctgcgc tgctgatgat
     6601 cgcgggaaat ctggcgggcc tcgccttaag catccccctg cgccccaacc cgatatcgtc
     6661 cggcggtctg gtcgacgcgt cgaccctgta cgccacccaa atcctgcaac gcgacaccac
     6721 cgacgacggc gaagacaaat gaggttgatt gcgtgtggct tgtaccacat tcaattccac
     6781 gccgattttc cggctcgatc gaggctgacg aactgatggc cgcaacaaac cccgaaagcg
     6841 gcgcaaggcg atgatcgatt catccaagat ctacggttgt cgaatcctga tcgtcggcga
     6901 ccggccggcc aacgttcggc ttctcgaaaa gatgctgcgg cgcgccggct acacctgcat
     6961 cgcttcaagc gcggacccga cggccgtctg cgagcttcat ctcaagaacc gctacgatct
     7021 tattctgctt gacctccagg aacgcagggc agacgggttc ctggttctgg aggccctcaa
     7081 gaaaatcgaa ccggaggatt atctctcggt cctcgccatc gcggcgcagt cggaccacaa
     7141 gcaacgggcc ctgcaagccg gagccaagga tttcatcagc aacccactcg accgttcgga
     7201 agtcctgacc cgcatctaca acatgctgga agtgcggttg ctgcacgggg agacgaaggc
     7261 ccacggcaag cttctcgaag aaaaggtgcg cgagcgcacg gccgaattgc gcagaagcga
     7321 ggaaatgttc cgcgaactcg cggccaacat tcccgaggcc ttgtggatca gggatatcga
     7381 ggggcaaacg ctccgctacg tgaacccggc gtggcaagaa ctgagtggct tgtcagccgc
     7441 acccggcgac ccgatcgata agctttatca aacggttcat cccgacgaca ggcaaagaat
     7501 cgccaacgaa cggcgcaggg cgccgaatac gcacgccagc agcgaataca ggctcgtgcg
     7561 ccccgatcgg agcgtgcgct gggttcatgc gcggacgttc ccgatcgcga atccttcggg
     7621 aaagtctcca tggctggtcg agatcctgga agacgtcacc caacgcagag aagtgcagcg
     7681 gcaacttgtg cacctcgcgc gccatgatgc gctcaccggc cttcctaacc gcatgtttct
     7741 gtacgaatcg ttgcaggagg ccctggtgca cgccagcgaa gatcacctgg tcgtgtcggt
     7801 tttgcttctc gacatcgact atttcaagaa cgtgaacgat acgctcggac acgcggtcgg
     7861 cgatgccgta ttgcgcgaat tcgcggcaag gctggcccaa tgcgtgcgcc ccggagacat
     7921 cgtcggccgc cttgccggcg atgaatttgc cgttatcgtc ctgacgcccg cggattccaa
     7981 cggcgccatc gacgttgcca acagaatacg ggattcgctg caaaacccgc ttgtactgca
     8041 agataagaat atcctggtaa cggcaagtgt cggcatcgca agctatccta ccgatacctc
     8101 cgatctcgaa gtgctcatcc gctatgcaga tgccgccttg tacgatgcca aggcggcagg
     8161 cagaaacggc ttccggcgct acacggcgga attgaacgcg cgcgcgctcg aaagggcgga
     8221 caccgagggc gccttgcggc ttgcgcaagg gcgcgaagag ttcctgttgt actatcagcc
     8281 gaaaatgcag atcgacaac
//
LOCUS       DASWPU010000484         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_83975,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000484 DASWPU010000000
VERSION     DASWPU010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83975"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VGC27_10255"
     CDS             <1..1615
                     /locus_tag="VGC27_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEY0282990.1"
                     /translation="SAQKLRGGYYTPADLATFLVRWVRHKAPVDILEPSCGDGVFFKP
                     LADLIPSAHVTGFEIDPAEAKKASKAARQAGLREVGIRRDDFLGWAGEALEGNREQFD
                     AVLGNPPFIRYQYLPPLFQGRAETIFSALDCKFTKHTNAWVPFILASFALLRPGGRLA
                     MVVPAEIIHVTHAQSLRSYLGANSSRLVVVDPEELWFEGTLQGAVLLMAEKKAHPDEH
                     AEGLGMYPVKGRDFLNLDPEDVFRAPQSINGKTVAGKWTRALLQPSTRSLLDGLVDSG
                     EVRRFDDVAEVDVGIVTGANKFFLVEDETVEQFGLQQWAHPMFGRSEHCPGVVYDERQ
                     HRANAIKGNPTNFIWFQDRSVEKSRTGKRYIEQGEKESLHTRYKCRIRSPWYSVPSVY
                     ATEIGMLKRSHDTPRLILNQLRAYTTDTAYRIRLKEGTAEQLVYGFVNALTALSAELE
                     GRHYGGGVLELVPSEIEKLLVPPPLKVRPKVAELDKAVRTLPMDAVLEQQTRRTLGGL
                     GLSAADQSELLNGWKRLRDRRHRVSADAD"
     gene            complement(1612..2808)
                     /locus_tag="VGC27_10260"
     CDS             complement(1612..2808)
                     /locus_tag="VGC27_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEY0282991.1"
                     /translation="MGRKILFQGVTPETHLAAVRHVLAIPNPQTVIFSTAFMNRGGLL
                     QIRESLEPVAAVTTIIAGIRNGITSAQGLLAALEFGCTAFAVDTGSRNVIFHPKIYFA
                     RNATAARLVLGSANLTIGGLNSNIEASVTMTLDLGVKDDADFVADVEAKIAGMRADYP
                     EHVFNVTDAAAVALLLEAGRVVDESIVAAPTPGGSSGNRDLDTIPKMKLKTPPIKRPV
                     MLPAAAAPAAPAPAPVAPAAVAAGAPVPAAPVRDQLSLVWESRPLTRRYLTIPTGANT
                     NPTGSMLFTKGVMDDIDQRHYFRDEVFNGLNWHLDTVPGKEHMERAEAQFRLIIRDVD
                     YGVFTLRLSHNTRTDTAAYEQSNSMTQLHWGEARPLVAREDLLDRTMYLYRDETDPDL
                     FVLEID"
     gene            complement(2965..3237)
                     /locus_tag="VGC27_10265"
     CDS             complement(2965..3237)
                     /locus_tag="VGC27_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HEY0282992.1"
                     /translation="MRTWQVERRRRTRHLIELGGLVVKAGIVDLTGDDRAMIYGALLW
                     VTHKLQSDEGEHARSLWAAKGKRAFDGERKGETSAVPQMPQDWAAR"
     gene            complement(3239..3565)
                     /locus_tag="VGC27_10270"
     CDS             complement(3239..3565)
                     /locus_tag="VGC27_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HEY0282993.1"
                     /translation="MRKPRDFDAELKSLEDKARDLKARKVQQLGELVISTGADALTAD
                     ELAGALVVLAETKDAGKREAWAKRGAAFFQSRARRSASAPDRDAGGAPAQPGGAQPAS
                     GGADAA"
BASE COUNT          713 a         1171 c         1160 g          795 t
ORIGIN      
        1 atcggctcag aagcttcgcg gcggatacta tacgcctgct gatctcgcga ccttccttgt
       61 gcgttgggtc cgtcataagg ccccggttga catccttgag ccaagttgcg gcgacggtgt
      121 ttttttcaaa ccgcttgcag acctgattcc gtctgctcat gtcaccggtt ttgagatcga
      181 cccggccgaa gcgaagaagg ccagcaaggc agcccgtcag gcgggcttgc gcgaggtagg
      241 aatccgccgc gacgactttc taggctgggc tggcgaagcg cttgaaggaa atcgggagca
      301 gttcgacgcc gttcttggaa atcccccctt cattcgctac cagtatttgc cgccgctttt
      361 tcagggccgc gccgaaacca tcttttccgc ccttgactgc aagttcacga agcacacgaa
      421 cgcatgggtg ccgttcattc tggcatcgtt cgctctgctg cgtccgggcg ggcggcttgc
      481 catggtcgtt ccggccgaga tcatccatgt tacacacgcg caatcgctgc ggtcctacct
      541 aggcgcaaac tcgagccgcc ttgtcgtcgt cgaccccgag gaattgtggt ttgagggcac
      601 gcttcagggc gcggtgctgc ttatggccga gaagaaagcg cacccggacg aacacgccga
      661 gggcctaggc atgtatcccg tcaagggccg ggatttcctg aatctggacc ctgaagacgt
      721 tttccgagcg ccgcagtcga tcaacggcaa gaccgtcgct ggaaaatgga cgcgggcgct
      781 gttgcagccg tccacccgtt cgctgctaga cggcctcgtt gatagcggcg aggttcgccg
      841 cttcgatgac gtggccgaag ttgacgttgg aatcgtcacc ggagccaaca aattcttttt
      901 ggtcgaggat gaaacggtag agcaattcgg cttgcaacaa tgggcccacc cgatgtttgg
      961 ccgaagcgag cattgcccag gcgtcgtata tgacgagcgt cagcaccggg caaacgcaat
     1021 caagggcaac ccgacgaatt tcatatggtt tcaggacagg agcgtcgaga agagccgcac
     1081 cggcaagcgc tatatcgagc agggtgaaaa agagagcctg cacacgcgct ataagtgccg
     1141 catccggtcg ccgtggtatt ctgtcccgtc agtttatgcg acggaaattg ggatgctgaa
     1201 gcggtcccat gacacgcccc ggcttattct caaccagcta cgtgcctaca cgaccgacac
     1261 agcttaccgc atccgcttga aggaaggcac ggccgagcag cttgtttatg gtttcgtcaa
     1321 cgcgctgacg gcccttagcg ccgagcttga gggccgtcac tacggcggcg gcgtcctcga
     1381 attggtccct tccgagattg aaaagctgct agtcccaccg ccgctgaagg tgcggccgaa
     1441 ggttgccgaa cttgataagg ccgtgcggac tttgccgatg gatgccgttc tagagcagca
     1501 gacgcggcgc acccttggcg gtcttgggct atcggccgcc gatcaatcgg agttgctgaa
     1561 cggatggaag cggttacgcg accggcgaca ccgtgtgtcc gccgacgctg attagtcgat
     1621 ttccaagacg aacaagtccg ggtccgtttc atcccggtaa aggtacatcg tgcggtctag
     1681 cagatcctca cgggcgacga gaggccgcgc ttcgccccaa tgcagttggg tcatgctgtt
     1741 gctttgctca taggcggcag tgtccgtccg cgtattgtga gaaagccgca gcgtgaacac
     1801 tccataatca acgtcgcgga taatcagccg gaattgcgct tcggcgcgtt ccatatgttc
     1861 tttaccgggc actgtatcaa ggtgccagtt caatccgttg aagacctcat cccggaaata
     1921 atgacgttgg tcgatatcgt ccattactcc tttggtgaac agcatcgagc cagtggggtt
     1981 cgtgttcgcc cccgtcggaa tcgtcagata gcgccgtgtc agagggcggc tttcccagac
     2041 aagggatagc tgatcacgca cgggcgcggc cgggacagga gccccagcag cgacggcggc
     2101 cggtgccact ggcgcgggag ccggggccgc aggagccgcc gctgctggca gcatgaccgg
     2161 gcgttttatc ggcggcgttt tcagcttcat tttcggaatc gtgtcgagat cgcgatttcc
     2221 cgacgacccg cccggcgtcg gggccgccac tatgctttca tcgaccacgc ggcccgcctc
     2281 gagcagcaag gccacggccg cagcgtccgt tacgttgaaa acgtgttccg ggtaatcggc
     2341 ccgcatccct gcgatttttg cctcaacgtc ggccacaaaa tccgcgtcgt cttttacgcc
     2401 gagatcgagc gtcatggtaa cgctggcctc gatgttggag ttaagcccgc caatcgtcag
     2461 gttcgcgctg cccagcacga gccgggcagc cgtcgcgttc cgagcaaagt agattttcgg
     2521 gtgaaagatc acgttgcggg agccggtatc aacggcaaac gccgtgcagc caaattcgag
     2581 cgctgcgagc agcccttgcg ccgaggttat cccgttgcgg atgcccgcaa tgatcgtcgt
     2641 gaccgctgcg acaggttcca gagattcacg aatttgcaga agaccacccc ggttcataaa
     2701 ggcggtacta aaaatcacgg tttgaggatt tggaatcgct aaaacgtgcc ggacggccgc
     2761 aagatgcgtc tcgggcgtta cgccctgaaa tagtattttt ctccccatca gccccaccga
     2821 ttcttactat ctcaacgata ctggcagtgc cgccaggagt catcatgtta caagtgagga
     2881 agctggtgcg ggatgcaccg cattgcgcgc caagcagacc gatgacgggc ggcgctgcga
     2941 tatgtaaggt ctccgcgcca cccgtcatcg cgctgcccaa tcctgcggca tttgtggcac
     3001 ggcggatgtt tctcccttcc gctccccatc gaatgcccgc ttccccttcg cggcccaaag
     3061 gctccgtgcg tgctcccctt cgtcgctctg gagcttgtgg gtcacccaaa gcagcgcgcc
     3121 gtagatcatg gcgcggtcgt cgccggtgag gtccacgatc ccggccttga cgacgaggcc
     3181 gccgagttcg atcagatggc gcgtgcgcct gcggcgctcg acctgccaag tgcgcatatc
     3241 atgccgcgtc cgcgccgcct gatgccggtt gtgcgccgcc cggttgcgct ggagcgccgc
     3301 cagcgtcgcg gtcaggcgct gatgcagatc gccgcgcccg gctttgaaag aacgcggccc
     3361 cgcgcttggc ccatgcctcc ctcttcccgg catccttggt ttcggccagc acgaccagcg
     3421 cgcccgccag ttcgtcggcg gtcagcgcgt cggctccggt cgagatgacc aactcgccga
     3481 gttgctgcac cttgcgggct ttcaggtctc gcgccttgtc ctcaagcgac ttcagttccg
     3541 catcaaagtc ccgtggcttg cgcatcatgt cctccataag cagtcgatgg agggatgata
     3601 gttttgtgcc tgcggaaatg ctgtaaggtt accgggacgg cctggaacgg cgcggatcat
     3661 cccgagaaat tagtttcgag ggcgcgctta tacgtcgttc cgacgtgcgc ttcgctgtgg
     3721 tgatgttcgg atcgcgatgg cgatctatca tcttcacgtc aaggtcatcg gccgcaaatc
     3781 cggctccagt gcggtggcct ccgctgccta ccgctcggca tcgcggctgc gcgacgatc
//
LOCUS       DASWPU010000485         5965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_101797,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000485 DASWPU010000000
VERSION     DASWPU010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101797"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VGC27_10275"
     CDS             complement(<1..313)
                     /locus_tag="VGC27_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY0282994.1"
                     /translation="MAGLRRTIALVGMMGAGKSSIGRRLAARLEVPFRDADGEIERAA
                     GCTVAEIFARYGEGAFREGERKVIERLLGEPPHVLATGGGAFIDPQTRTRIKDSAVSV
                     WI"
     gene            401..568
                     /locus_tag="VGC27_10280"
     CDS             401..568
                     /locus_tag="VGC27_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282995.1"
                     /translation="MRIARSEGLSWGAWVAIGLGALAILAAVGLSVYGGSVHPKMHEI
                     RQVLSNDRFPA"
     gene            585..2363
                     /locus_tag="VGC27_10285"
     CDS             585..2363
                     /locus_tag="VGC27_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0282996.1"
                     /translation="MALLAPAAWAQTPPPDGDVPPVLTEEDYPPLAGEDDDLPAASAP
                     PQALTETETETQPESQLPPPQGAPVEVGVLGTTEGPPVGLLDSANGGLGESLWSGSKR
                     AEIEELLTRIPLVSPDPAIRALAKRIVLTIAAAPPGPSKRALVAVRIEKLLDAGLIDE
                     AGALAAQASVPNDPDFARVQAEALLTASRAADVCGEATATRLTAGEPFWLQLRAYCAA
                     AGGDRPLADLTLAVLEAQGSSDKAYNTLIDDVLNHKSNPPGPIAHPSAMHLFLLRQAG
                     LPVPGEVASVMGTPANLLAMRDARNSPPVRFAAAERIVRTGATSVTELRMLADAQDIP
                     LSRLANAIADAQAAPFFAGQVLLRRAAVIEPRPDAKAQLVFEALTLGARAGLLPLAAG
                     LQGDIAATVKPDPANRAMADLFARALLLARLPDAAARWLSDKDVLFTAVDLAAPNPVR
                     NAKAQAAFSTMAAALIKDPPGQDPDKPAKALLLGIADVLGHPLPPDAKAHAPGIEAMD
                     WGGKRLDPAIMQRIESAALHPDRKGEALLLILDTIRSVGLRDLAPKDTIELIRLLGAM
                     GLADAAHDIGIEALALYVPPPPAPPK"
     gene            2360..3316
                     /locus_tag="VGC27_10290"
     CDS             2360..3316
                     /locus_tag="VGC27_10290"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HEY0282997.1"
                     /translation="MSRSAGGARLIEAFLDMMSAERGASANTLAAYRRDLLDFHGFLA
                     GRGSEAKRAARDDVKNYLGSLSASGIAGSSQARRLSALRQFFGFLYAEGVRPDDPTDA
                     IDAPKRGRTLPKILSREDMALLIEAVERSAAQSEEGARLRCIVEMLYASGLRVSELAT
                     LPLAAVRSRDGVLLVKGKGGKERLAPLHPRALQAIADYLVVREMFLPRGDRRRLAERF
                     LFPSRSAECHLTRRRLHQMLKALALDAGLDPAKLSPHVLRHAFATHLVEGGADLRSVQ
                     TMLGHADIATTQIYTHVARDRLAATVASAHPLAKRKPGRALD"
     gene            3541..4521
                     /locus_tag="VGC27_10295"
     CDS             3541..4521
                     /locus_tag="VGC27_10295"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0282998.1"
                     /translation="MQTVNLGKTGLEVSRICLGCMTYGVPGRGMHGWTLDEENSRPLI
                     KKALELGVNFLDTANVYSDGSSEEIVGRAIKDFAKREDIVLATKVHGRMRPGPNGGGL
                     SRKAIFAEIDNSLRRLGTDYVDLYQIHRWDYATPVEETLEALHDVVKAGKARYLGASS
                     MYAWQFAKALYVSRLNGWSEFVSMQNHVNLLHREEEREMLPLCADQGIGVIPWSPLAR
                     GRLTRDWNETSERQKNDNFGSTLYASMQDADRKIVAAVAEVAKARGVPRAQVALAWVA
                     QKSVVTAPIVGASKSLHLDDAVAALSLNLTPDEIARLEAPYVPHPVVGFS"
     gene            complement(4708..5433)
                     /locus_tag="VGC27_10300"
     CDS             complement(4708..5433)
                     /locus_tag="VGC27_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0282999.1"
                     /translation="MSPKSAPNGLRRLWHAVRFLAPADIALFLKSAKTDAAIARWRIE
                     LGSRAAFENAYGGDGDPWAAASPRYRYQSWKYDRLVALLPKGRRFAHALDLGCGLGLL
                     STRLAARADQVTGIDIAQAAVDRAAARASAENVHFQQGDVTDIPSSLDGLFDLVVIAD
                     VLYYLPPPITAEVLGKIAARIAKLLVPGGLCVLVNHYFFSLDPDSRLSRRIHGAFITS
                     PHFRVVSQHRRPFYIVSMLDKAG"
BASE COUNT         1051 a         1937 c         1910 g         1067 t
ORIGIN      
        1 tgatccagac cgacaccgca ctgtctttta tccgggtacg cgtttgcgga tcgataaagg
       61 ccccgccgcc ggtcgccagc acgtgcggcg gctcgccgag cagccgctcg atcaccttgc
      121 gttcgccctc gcggaacgcg ccttcaccat aacgggcgaa gatttccgcc accgtgcagc
      181 cggcggcgcg ctcgatctcg ccgtcggcgt cccgaaacgg cacttccagc ctggccgcaa
      241 gcctgcgtcc gatggagctc ttgccggctc ccatcattcc gaccaatgct atggtgcgcc
      301 tcaaacctgc catatcctcc cgctaccacg gcaaggcgga gcgtcgccag tatgacttgc
      361 ggccgattcc gcgaaaatcg gctgcaaaag aggtatagaa atgcgtattg caaggtctga
      421 gggcttgagt tggggcgcct gggtggcgat aggcttgggg gcgctggcga ttctggcggc
      481 cgtcggtctg tcggtctatg gcggctcggt gcatcccaag atgcatgaga ttagacaggt
      541 tctgtcgaat gacaggtttc cggcttagaa taattccact tctcttggcg ctcttggcgc
      601 cggccgcctg ggcgcagact ccgccgccgg atggggacgt tccgccggtc ttgacggaag
      661 aggattatcc gccgctggcg ggcgaggatg acgatctgcc cgcggcctcg gccccgcccc
      721 aggcattgac cgaaaccgaa accgagactc agccggagtc gcaattgcct ccgccgcagg
      781 gtgctcccgt cgaggtgggc gtccttggca cgacggaagg cccgccggtc gggcttctcg
      841 attcggccaa tggaggcctc ggcgagagcc tgtggagcgg gtccaagcgg gcagaaatcg
      901 aagagctgct gactcgtatt cccctggttt cgcccgatcc ggcgattcgc gcgctggcca
      961 agcgcatcgt cctgaccata gccgctgcgc cgcccggtcc gtccaagcgt gcgctggtcg
     1021 cggtccgtat cgaaaaactg ctcgacgccg gcctgatcga cgaggccggt gcgcttgcgg
     1081 cccaggcttc ggtccccaac gatcccgatt tcgcgcgcgt ccaggccgaa gcgctgctca
     1141 ccgccagccg cgccgccgat gtgtgcggcg aggcgaccgc cacgcgcttg accgcgggcg
     1201 agccgttctg gctgcaactg cgcgcctatt gcgcggccgc cggcggcgat cggccgcttg
     1261 ccgatctcac ccttgcggtc ctcgaagcgc agggcagttc cgacaaggct tacaacacgc
     1321 tgatcgacga cgtgctgaac cataagagca atccgcccgg cccgattgcg catcccagcg
     1381 cgatgcatct gttcctgctc cggcaggcgg gcttgcccgt tcctggcgag gtcgcttccg
     1441 tcatgggcac gccggccaat ctcctggcga tgcgcgacgc gcgcaattcg cctccggtac
     1501 gctttgccgc cgccgaacgc atcgtgcgga cgggcgccac ctccgttacc gaattgagaa
     1561 tgctggccga cgcccaggac attccgctct ctcgccttgc caacgccatc gccgacgcgc
     1621 aagccgcgcc tttctttgcc ggtcaggtgc tgttgcgccg cgcggccgtc atcgaaccgc
     1681 gccccgatgc caaggcgcag cttgtgtttg aggcgttgac gcttggcgcg cgggccggac
     1741 tgctgccgct ggcggccggg ctgcaaggcg acatcgccgc tacggtcaaa ccagatcctg
     1801 caaaccgggc catggccgat ctgttcgcgc gcgcgctgct gctggcccgc ctgcctgatg
     1861 ccgccgcgcg ctggctgagc gacaaggacg tgctgttcac cgctgtcgat ctggcggcgc
     1921 ccaatccggt ccgcaatgcc aaggcgcaag ccgctttcag caccatggcc gcagcgttga
     1981 tcaaagatcc gccggggcag gatcccgaca agccggccaa ggctctgctg ctgggcatcg
     2041 ccgacgttct ggggcatccg ctgccgcccg acgccaaggc gcatgcgccc ggaatcgaag
     2101 cgatggattg gggcggcaag cggctcgatc ccgcgatcat gcaacgcatc gagagcgccg
     2161 ctctgcatcc cgaccgcaaa ggcgaagcgc tgctgctgat cctggatacg atccgctccg
     2221 tcggcctccg cgatttggcg cccaaggaca ctatcgagct gatccgtctg ctcggcgcga
     2281 tggggcttgc ggatgcggcg cacgatatag ggatcgaggc gctggcgctt tacgtcccgc
     2341 cgcctccggc gccgccgaaa tgagccgcag cgcaggcggc gcgcgcttga tcgaggcgtt
     2401 tctcgacatg atgagcgccg agcgcggggc cagcgccaac acgcttgccg cctatcgccg
     2461 cgatctgctg gactttcacg gctttctcgc ggggcgcggc agcgaagcca agcgcgccgc
     2521 acgcgatgac gtcaaaaact atcttggcag cctgtcggct tcgggcatcg ccggttcgtc
     2581 gcaggcgcgg cggctttcgg cgctgcggca gttcttcggc tttctctatg ccgaaggcgt
     2641 ccgtcccgac gatcctaccg acgccatcga tgcgcccaag cgcggccgca cgctgccgaa
     2701 aatcctttcg cgcgaggata tggccctgct gatcgaagcg gtggaaagaa gcgccgcgca
     2761 aagcgaagag ggcgcgcggc tgcgctgcat cgtggaaatg ctctatgcct ccggcttgcg
     2821 cgtttcggag ttggcgacgc tgccgctcgc cgccgtgcgc agccgcgacg gcgtgctgct
     2881 ggtcaaaggc aaaggcggca aggaaaggct ggcgccgctg catcctcgcg cgcttcaggc
     2941 catcgccgat tacctcgtgg tgcgagaaat gttcctgccg cgcggcgacc gccgccgcct
     3001 cgccgagcgg ttcctgtttc cgtcgcgctc ggcggaatgc catctgacgc gccgccgcct
     3061 gcatcagatg ttgaaggcgc tggcgctgga cgccggcctc gatcccgcca agctgtcgcc
     3121 gcacgtgctg cgccacgcct tcgccaccca tctggtggaa ggcggcgcgg acttgcgcag
     3181 cgtgcagacc atgctgggac acgccgacat cgccacgacg caaatctata cgcatgtggc
     3241 gcgcgaccgc cttgccgcga cggtcgcctc cgcccatccg ctggcgaagc gcaagcccgg
     3301 ccgcgccctg gactgaggcc gcgcgaattt cccgaaattg gtggaaacga gcgctccgcc
     3361 tcgctattta agcttcaaga ttccgccaca cagacaggaa gttgacgatg gcagcaaaca
     3421 tttcggcggc cgcttcgggg caattcaaga tcggcggcga cctcgccgtg aaccgcctcg
     3481 gcttcgccgc gctcgaccgt caaggcaaga acgccgcctg acaacataag gacgtctgca
     3541 atgcaaaccg tgaatctcgg caagacgggg cttgaagtat cgcgcatctg cctgggctgc
     3601 atgacctatg gtgtgcccgg ccgcggcatg cacggctgga ctctggacga agagaacagc
     3661 cgcccgctga tcaagaaggc gctggaactg ggggtcaatt tcctcgatac ggcgaacgtc
     3721 tattcggacg gctcctccga agagatcgtc ggccgcgcga tcaaggactt cgccaagcgc
     3781 gaggatatcg tgctcgccac caaggttcac ggacgcatgc ggcctgggcc gaacggcgga
     3841 ggcttgtcgc gcaaggcgat tttcgccgag atcgacaaca gcctgcgccg gctgggcacg
     3901 gattatgtgg acctctatca gatccatcgc tgggattacg ccacgcccgt cgaggaaacc
     3961 ctggaagcgc tccacgatgt cgtgaaggcg ggcaaggcgc gttatctcgg cgcctcgtcg
     4021 atgtacgcct ggcagttcgc caaggcgctt tacgtctcgc ggctgaacgg ctggagcgaa
     4081 tttgtttcca tgcagaacca cgtcaatctg ctgcatcggg aagaggaacg cgaaatgctt
     4141 ccgctctgcg ccgatcaggg tatcggcgtc attccgtgga gtcctctggc gcgcgggcgg
     4201 ctgacgcgcg actggaacga aaccagcgag cggcagaaga acgacaattt cggttcgacg
     4261 ctttacgcct caatgcagga cgccgaccgc aagatcgtgg cggcggtggc cgaggtcgcc
     4321 aaggcgcgcg gcgtgccccg cgcccaggtg gcgctggcct gggtggcgca gaagagtgtc
     4381 gtcaccgcgc cgatcgtcgg cgcatcgaag tcgcttcatc tggacgatgc ggtcgcggcg
     4441 ctgtcgttga atctcacgcc tgacgagatc gccaggctgg aagcgcctta cgttccgcat
     4501 ccggtggtcg gattttcgtg aggcgggact tgagcgtacg gcatcgaggc gacgcaagaa
     4561 aactcagtgt catggcccgc atcctgagca gcgaaggcgc cgcccttcga caagctcaag
     4621 gtgacgggat cagctaaacc ctcatgctga gccctcatcc cgagcttgtc gagggacgaa
     4681 gcatgaggct ggcgcggtca cgccgcttca acccgcttta tcgagcatgc tgacgatgta
     4741 gaacggcctt cggtgctgtg acaccacgcg gaaatggggc gaggtgatga aggcgccgtg
     4801 gattcttcgc gacaggcggg agtccgggtc cagcgaaaag aaataatgat tgaccagaac
     4861 gcaaagcccg ccgggcacca gcagtttggc gatgcgcgcc gctatctttc ccagcacctc
     4921 cgccgtgatc gggggcggca ggtaataaag cacgtcggcg atcacgacga gatcgaatag
     4981 tccgtcgagc gagcttggaa tatccgtcac gtcgccttgc tgaaaatgca cattctcagc
     5041 agaggcgcgg gccgccgccc ggtccacggc cgcttgtgcg atgtcgatgc cggtcacctg
     5101 atcggcgcgc gccgccagcc tcgtgctcaa gaggccgagg ccgcagccga gatcgagagc
     5161 gtgggcaaag cgccgtcctt tcggcagcag agcgacgagg cggtcgtact tccagctctg
     5221 atagcgatag cgcgggctgg ccgcggccca gggatcgccg tcgccgccat aggcgttctc
     5281 gaaggcggcg cgcgaaccca attcgatgcg ccagcgcgcg atggcggcgt cggtcttggc
     5341 gcttttcagg aacagggcga tatcggccgg cgccagaaag cgcacggcat gccaaagccg
     5401 ccgcagtccg tttggagctg atttggggga catagaactt gatatgctcc ttgcggccga
     5461 cagcgactcc gttccgcggc ttatcccaca tttctcaatc tgccgtaaaa ttgcgttcgc
     5521 tgggccgggc gcagcttcaa taagttcgcg cgaagatcag ggtcggtgtg agaattgact
     5581 tcagacaccg ccgctggtcc acatcgccgc acgggcttga agacgccgcc atgagtttcc
     5641 acatctcttc cgtgttgttg gccgaaggag gttcgggaaa caaagcgtgg cgcgcagaga
     5701 tggcagttcg tgtcacgtct gcgaagcttg ttccggtttc gcttcaaaga cttgaacgcg
     5761 tttttcggga ttcgaattca aaaaacggaa agggaggggc acccccctga aattcagcgg
     5821 aacaattata ttgcggaatg ttttataaca ctttgaatat caaagaataa ttcctatatc
     5881 cgtttaaacg ccgccttgcc gccgccggct tctttacctc agattgacac gcctgagcga
     5941 ccttgccact ttcccggccc ttttg
//
LOCUS       DASWPU010000486         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_41498,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000486 DASWPU010000000
VERSION     DASWPU010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41498"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /gene="dxs"
                     /locus_tag="VGC27_10305"
     CDS             complement(<1..1661)
                     /gene="dxs"
                     /locus_tag="VGC27_10305"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY0283000.1"
                     /translation="MSIPTKTPSLDCVSTPSDLRALPRSGLRQLAGEVRKELVDVVSV
                     TGGHLGSNLGVVELTVALHYVFDTPHDRLIWDVGHQAYPHKILTGRRSRMRTLRQGGG
                     LSGFTKRAESEYDPFGAAHSSTSISAGLGFAVARDLQEQSHNVVCVIGDGAMSAGMAY
                     EAMNNAGASKARLIVVLNDNDMSIAPPVGAMSAYLARLVSSRPYRGIRALAKRLARYL
                     PRPMEISARRAEHYARGMVTGGTLFEELGFYYVGPIDGHNLDHLIPVLENVRDMKNGP
                     ILVHVVTKKGKGYPPAEASDDKYHGVTKFDVLTGEQKKPVSNLPSYTKVFSDALIAEA
                     RKDKRIVAVTAAMPTGTGLDRFAEEFPGRCFDVGIAEQHAVTFAAGLAAEGMKPFCAI
                     YSTFLQRAYDQVVHDVALQSLPVRFAIDRAGLVGADGATHAGAFDVAYLATLPGFVVM
                     AAADEVELMHMVATCAAIDDGPSAVRYPRGDGIGLERPPFGTPLEIGKGRIVREGHKV
                     ALLSFGARLGECLKAADELASFGLSTTVADARFAKPLDTDLVLRLA"
BASE COUNT          314 a          587 c          576 g          289 t
ORIGIN      
        1 gcgaggcgga gcaccagatc ggtgtcgagc ggcttggcaa accgggcgtc ggcgaccgtc
       61 gtcgacaggc cgaacgaagc gagctcgtcg gctgccttga ggcactcgcc gagccgcgcg
      121 ccgaacgaca gcagcgcgac cttgtggcct tcgcgcacaa tacggccctt gccgatttcg
      181 agcggcgtgc cgaacggcgg ccgctccagg ccgatgccgt cgccgcgcgg atagcgcacc
      241 gcgctggggc cgtcgtcgat ggcggcgcag gtagccacca tgtgcatcag ctccacctcg
      301 tcggccgccg ccatcaccac gaaacccggc agcgtcgcca aataggccac gtcgaaggct
      361 ccggcatggg tggcgccgtc ggcgccgacc agccccgccc ggtcgatcgc gaagcgcacc
      421 ggcagcgact gcagcgccac gtcgtgcacg acctgatcgt aggctcgttg caggaaggtc
      481 gaatagatcg cgcagaacgg cttcatgccc tcggcggcaa ggcccgccgc gaacgtcacc
      541 gcatgctgct cggcgatgcc gacgtcgaag cagcggccgg gaaattcctc ggcgaaacgg
      601 tcgagcccgg tgcccgtcgg catcgcggcg gtgacggcga cgatgcgttt gtctttccgc
      661 gcttcggcga tcagcgcgtc ggaaaagacc ttggtatagc tcggcagatt cgagacgggc
      721 ttcttctgct cgccggtgag cacgtcgaac ttggtgacgc cgtgatattt gtcgtcgctc
      781 gcctcggcgg gcggatagcc cttacccttc ttcgtcacca catgaacgag gatcgggccg
      841 ttcttcatgt cgcggacgtt ttccagcacc gggatcaggt gatcgaggtt gtggccgtcg
      901 atcgggccga cgtaatagaa gcccaattcc tcgaacagcg tgccgccggt gaccatgccg
      961 cgcgcataat gttccgcccg ccgcgcgctg atctccatcg gccgcggcag ataacgggcg
     1021 aggcgcttgg cgagcgcgcg gatgccgcga tacggccggc tcgacaccag gcgcgcaaga
     1081 taggcgctca tcgcgccgac cggcggggcg atcgacatat cgttgtcgtt gagcacgacg
     1141 atcagccgcg ccttagatgc gcccgcattg ttcatcgcct cgtaggccat gcccgcgctc
     1201 attgcgccgt cgccgatcac gcagacgaca ttgtgcgatt gctcttgcag gtcgcgcgcc
     1261 acggcgaaac cgaggccggc cgagatggac gtggaggaat gcgcggcacc gaacggatcg
     1321 tattcgctct cggcgcgctt ggtgaaaccg gaaagcccgc cgccctgacg caaggtgcgc
     1381 atgcgcgaac gccggccggt gagaatcttg tgcggatagg cctgatggcc gacgtcccat
     1441 atcaaccggt cgtgcggcgt atcgaagaca tagtgcaggg cgaccgtcaa ttcgacgacg
     1501 ccgaggttcg aacccagatg acctccggtc accgatacga catcgaccag ttccttgcgc
     1561 acctcgccgg cgagttgacg aagcccggaa cgcggcagcg cacgcaaatc gctcggcgtg
     1621 ctaacacagt ccaacgaggg cgtcttggtc gggatgctca tactgatctc tggtcccccg
     1681 cgctctgaaa gcgcgccgcc ccttgttctt cgtggccgcg cgcaaaaggt cagccttttc
     1741 aacggataaa tcaaggcaac cgacgg
//
LOCUS       DASWPU010000487         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_71854,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000487 DASWPU010000000
VERSION     DASWPU010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71854"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1870)
                     /locus_tag="VGC27_10310"
     CDS             complement(<1..1870)
                     /locus_tag="VGC27_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEY0283001.1"
                     /translation="MGAALAAFVLAARPALAASFTAGQETVLTVIAAGAAALAIAGGI
                     WALSEQNVSAKLRRMLRVAGARTRAAVGERDALLGAAREALIVWGRDGAGPYSYGGGE
                     ALLESCLKGAQATELSAALDALSEHGAAFALSVQDAQERRVSVRGRAVGGMAAVWIEP
                     ETKAAAATDFRAILDALPVPVWLRDRTMSLVWGNRAFLAASGAADLEAATREQVALEK
                     TERDLASSARTQGRQVEAKRYAIAAGQRRALAITEVPVDAGVVGTASDVTDVVAGEAR
                     LQQHIDAHTDTLDKLATAVVIFDREQKLSFYNRAFAKLWNLPEAWLDTHPGDGDILDR
                     LREAGKLAEQRNYQTWKRERLALYQTARDHPSEELWHLPGGKTLRVVAQPHPFGGLTF
                     LYEDVTERILLESSYNTLIKVQSATLNTLQEGVAVFGPDGRLKLHNAAFAKIWDLSDK
                     DLTGEPHVRAIAAACADKFGDASVWEKLIQNIVSGANLQRDMGEIERGDRTILSLALA
                     PLPDGATLATFADVTDRFRIEGVLRERAEALEAADRLKSDFIKHVSYELRTPLNTIVG
                     FAELLSGEDFGPLNERQSAYMKDIVAASNTLTSLVSDILDLALVESGALRLELERID"
BASE COUNT          359 a          708 c          725 g          393 t
ORIGIN      
        1 gatcgatacg ctccagttcc agccgcagcg cgccggattc gaccagcgcc aagtcaagaa
       61 tgtcggagac caggctggtc agcgtgttcg aggccgcgac gatgtccttc atataggcgc
      121 tctggcgctc gttcagcggg ccgaaatcct cgcccgaaag cagttcggcg aagccgacga
      181 tggtgttgag cggcgtgcgc agctcatagg acacgtgctt gatgaaatcc gatttcagcc
      241 ggtcggcggc ttccagcgct tcggcgcgtt cgcgcagcac gccctcgatg cggaaacggt
      301 cggtgacgtc ggcaaaggtg gccagcgtgg cgccgtcggg caggggcgcc agcgccaacg
      361 aaaggatggt gcggtcgccc cgttcgatct cgcccatgtc gcgttgcagg ttggcgcccg
      421 agacgatgtt ctggatcagc ttttcccaca ccgatgcatc gccgaatttg tcggcgcagg
      481 ccgcggcgat ggcgcggaca tggggctcgc ccgttaaatc cttgtcgctt aagtcccaga
      541 tcttggcgaa ggcggcgttg tgcagcttga gcctgccgtc ggggccgaac acggcgacgc
      601 cttcctgcag cgtgttgagc gtcgcggact gcaccttgat cagcgtgttg taggagcttt
      661 cgagcaggat gcgctcggtc acgtcctcgt aaaggaaggt caggccgccg aagggatgcg
      721 gctgcgccac cacccgcagc gtcttgccgc cgggcagatg ccacagttct tccgaggggt
      781 gatcgcgcgc ggtctgatag agcgccagcc gctcgcgctt ccaggtctgg tagttgcgct
      841 gctccgcgag tttgccggcc tcgcgcaggc ggtcgaggat gtcgccgtcg cccggatggg
      901 tgtcgagcca ggcttcgggc aggttccaca gcttggcgaa ggcgcgattg tagaagctca
      961 acttctgctc gcggtcgaag atgacgaccg ccgtcgccag cttgtcgagc gtgtcggtat
     1021 gggcgtcgat atgctgttgc agtcgcgctt cgccggcgac gacatcggtc acgtcgctcg
     1081 ccgtgccgac gacgccggcg tccaccggca cttcggtgat ggcgagcgcg cggcgctgtc
     1141 ccgccgcgat ggcgtagcgc ttggcttcga cttgccggcc ttgcgtacgg gccgaggagg
     1201 ccagatcgcg ctcggttttc tccagcgcca cctgctcgcg ggttgcggct tccagatcgg
     1261 cggcgcctga cgccgccaga aaggcgcgat tgccccagac cagcgacatg gtgcggtcgc
     1321 gcagccacac cggaacgggc agggcgtcga ggatggcgcg gaaatccgtg gcggccgccg
     1381 ctttggtttc gggttcgatc cagaccgcgg ccatgccgcc gacggcgcgc ccgcgcacgc
     1441 tgacccggcg ttcctgcgcg tcctggacgc tcagagcgaa cgccgcaccg tgctcgctca
     1501 gcgcatcgag cgcggcggac aattccgtcg cttgcgcgcc tttcagacaa gactccagca
     1561 gcgcttctcc gccgccgtag gaatagggtc cggcaccgtc gcggccccag acgatcaagg
     1621 cctcgcgcgc tgcgcccagc aaggcgtcgc gctcgcccac agccgcgcgc gtgcgcgccc
     1681 cggcaacccg gagcatgcgg cgcagcttgg ccgagacgtt ctgttcggac agtgcccata
     1741 tcccgccggc gatggccaat gcagccgcgc ctgcggcgat gacggtcagc accgtctctt
     1801 gtccggcggt gaacgaagcg gccagggcag ggcgggccgc caaaacaaac gcggcgaggg
     1861 cggcgcccaa gcctcgttgt cgtccgacca taaccccggt acccgggttt ttgttttgtc
     1921 ccattttggg acccatcccg gcttcccccg actcgccgcc ccatgcggcg aatcactgtg
     1981 gaatcaagat gatacgggcg ttaaccgttc gcgcaataga atgtggttaa gaaagtgtaa
     2041 aaaaccagat atggttttct ctgccggatg ttggggtaat agttaatttc cgagtcaaaa
     2101 cagtggccta aaagttccga ccacggaggg agtcccccac agaaaataat ctcgcggccc
     2161 cctgcgcgtt agccattctg ccatc
//
LOCUS       DASWPU010000488         5064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_89746,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000488 DASWPU010000000
VERSION     DASWPU010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89746"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VGC27_10315"
     CDS             complement(<1..839)
                     /locus_tag="VGC27_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0283002.1"
                     /translation="MSVIKRNSFAKLTQKDASAAAARHEMLYSGRMGGARAVFAFSDL
                     SGLDLSGRNLADADFTGALLEETNFAGARLDSASFFGADLRRANLAHASLRRGDLRGV
                     CLRGANLIGADLYEADLREGTIAEKDRYGNLRVLEHDIGPSEMPSALLNSANLERAKM
                     TGVIAAQADFTDAMMKGCRLSRANLHQAKLAGANLENADLSGCNLTGASLAGAVLVGT
                     RMDLALTEGADLSGALTGETAGSEPLPLPVSMDELLEGHARCVESGGREGKPADLSGM
                     DLRA"
     gene            1086..4724
                     /locus_tag="VGC27_10320"
     CDS             1086..4724
                     /locus_tag="VGC27_10320"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEY0283003.1"
                     /translation="MSKQSLMLSAAMAALLAGPAYAACTESTATLHCVTTSTTTALTT
                     GDPWTTSTVTTAVGTPNVGNIDIESGGAVTISTANIGAITVNSNHYVYSNGGITNEKQ
                     SNASAILIDVSKNPDLSGASFVNAAGTTITGAGIYLDAASTLNLTGTGTGKRAIFLDG
                     SNVATDATGTYTGDIIFNTGSNTVIAGDSSVGVLIANNAILHGNLNLGGTFTVTQTTA
                     SSTTASGLYGLHMLGQVQGNILLPSGGSMTILGAGAQGMSILGSGVTGSITIGGTLAT
                     GAPAPTTSSYYTNTSKIVYPEAGAALTIGAGVGNGIAILGNTYAGQGLASGVVATQGV
                     SPAVVIGPNTTLTSPMRIGVYTTDGTPAGGDAVDPGFSFVNRGSISATPANVANSASS
                     ASTSATGIAMGGSSVVYPVLVGGFFNSGTLSAGVQTTGTVAPAVSATALNIGGYTTFV
                     DATYDTATNSWNNLGTLTGKKDYGDSLAAPALAAPALVNSSATGGGTISATVSGSRGG
                     IATAISIASTANVPSIFNSGTISAAVSNSDPTLDGQANSHPVAAYAIVDQSGTLNSIY
                     NEGTISAAVSALDNGSQIANAILLGNRATGTQVRIDNVSTQSRSATIAGNISFGSGGT
                     QILNIEGTGTHLASVTGNVTFNGTGTGGDQLTIGSYGSLSGTIIAHNGVSVDIAQNGL
                     LTLANTTAPLYATTFAVHGGTTPENGGALVIGVSNTLGSQGVISATGSVEIERNARLG
                     LSFNSFVPQGSPDFVLITAPLGQLNVVDLASYSDAIGKSNQSCTNAPTSDCGGSRPFL
                     FASAELKKVSDAASDRLVLQVVPKTATELNLTGYAKQLFPYANTALGVDTTLGAAMVN
                     LIRNEAQAQAAYNSFAPNVTGGSRAIAISITDQATGAVGARLRMLNLYGKQEGGTTLW
                     SQEFFQMIKDPGQGALQLDGTKEKSGFKDHGFGFALGIDGGTPKYGWYGAALTFYTGD
                     VGEIVRNSHTNQQWYVLSGYSSWRGKGLFLNTKIDAGYGNFDSKRYITLQTDPNTIYS
                     RRADSKHAGALISGVVTTGALFSYGATTLSPQLSVDGLLMREEGYTEKNPGSATVGDG
                     FNLKVQPYYAKSLRLFLGGSVRYDIDLWDFYLQPEAHTGYRYDVFNDPAKLKAAFAHA
                     DLSGGSAVPGTQFTLTGPDPSQGNFVLGGSLATTTDTWTLGVNFDFVRGSNGALQQVG
                     TINLLGRI"
     gene            complement(4775..>5064)
                     /locus_tag="VGC27_10325"
     CDS             complement(4775..>5064)
                     /locus_tag="VGC27_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283004.1"
                     /translation="TGTRWLDHAARANGLARKLAAGLSAVPGAELAHPVQANAVFIYL
                     PDATVARMRRLGASFYDWGPPEGGRTLVRLVTSFATPDEDVQRFVEMAHGE"
BASE COUNT          987 a         1704 c         1532 g          841 t
ORIGIN      
        1 gcccgcaaat ccatgccgga gagatcggcg ggcttgcctt cgcgcccgcc gctctcgaca
       61 cagcgcgcgt gcccttccag caattcgtcc atggagacgg gcaacggcag cggttcgctg
      121 cccgccgtct cgccggtcag cgcaccggaa aggtcggcgc cctcggtcag cgccaggtcc
      181 atgcgggtgc cgaccagcac cgcgccggcc aggctggccc cggtcaggtt gcagccggaa
      241 aggtcggcgt tttcgagatt ggcgcctgcc agcttggcct ggtgcagatt ggcgcgcgac
      301 aggcggcagc ctttcatcat cgcgtcggtg aaatcggcct gggcggcgat cacgccggtc
      361 atcttggcgc gttcgagatt ggcggaattg agcagcgccg acggcatttc ggacgggccg
      421 atatcgtgct ccagcacccg caggttcccg tacctgtcct tttcggcgat ggtgccctcg
      481 cgcaggtccg cttcgtaaag gtcggcgccg atcagattgg cgccgcgcag gcataccccg
      541 cgtaaatcgc cgcggcgcaa gctggcatgc gccagattcg cccgcctcag atcggcgccg
      601 aagaaactgg ccgaatccag acgcgccccg gcgaaattcg tctcttcgag aagggcgccg
      661 gtgaagtcgg cgtcggccag gttgcggccc gacaggtcta gtccgctcag gtcggagaaa
      721 gcgaaaacgg cgcgcgcgcc gcccattcgc cccgaataaa gcatttcgtg gcgcgccgcg
      781 gcggcggatg cgtctttctg cgtcagcttg gcgaagctgt tgcgtttgat gacactcatg
      841 gacgtatcgc gaccggctgg ttaactgtca cccggggacc tattctcggc acaaccgcct
      901 aatagctgat taaaaagcgg aaatttgcct ttgtccgcac cgggcgctag gtgaggcgat
      961 ggaaattggt tatggtcgcg ccggctcgcc ggttgtcggg ggaaaccgcg cggctctaac
     1021 gggacggcaa taccagggcc gggcgcgcgc ctggccgcgg gccgcacaga cgcaagaggt
     1081 tgcacttgag caagcagagc ttgatgcttt ccgccgccat ggccgcatta ctggccgggc
     1141 ccgcttacgc cgcctgcacg gagtccaccg ccactctcca ctgcgtcacc acgagcacga
     1201 ccacggcgct tacgacgggg gatccgtgga ccacctccac ggtgacgacg gccgtcggca
     1261 cccctaacgt cggcaacatc gacatcgaaa gcggcggcgc cgtcaccatc agcacggcca
     1321 atatcggcgc catcacggtc aacagcaatc attacgtcta cagcaacggc gggatcacca
     1381 acgaaaagca aagcaacgcc tccgccatcc tgatcgacgt gtcgaagaat ccggatctga
     1441 gcggcgcttc gttcgtcaac gccgccggca ccacgatcac cggcgccggc atctacctgg
     1501 acgccgcgtc caccttgaac ctgaccggta ccggcacagg caagcgcgcg atcttcctgg
     1561 acggcagcaa cgtcgcgacc gatgctacgg gcacctacac cggcgacatt attttcaaca
     1621 ccggctcgaa cacggttatc gccggcgatt cgagcgtagg cgtccttatc gccaacaacg
     1681 cgatcctgca cggcaatctg aatctcggcg gcacattcac ggtgactcag acgacggcct
     1741 caagcacgac cgcttcgggc ctttacgggt tgcatatgct gggtcaggtc caaggcaaca
     1801 ttctgctgcc gtccggcggc agcatgacca tcctgggggc gggcgcccaa gggatgtcga
     1861 tcctgggcag cggcgtcacc ggcagcatta cgatcggcgg cactctcgcg acgggcgcgc
     1921 cggcgcccac gacctcgtcg tattacacga acaccagcaa gatcgtatat cccgaagccg
     1981 gcgccgcgct gacgatcggc gccggcgtcg gcaacggcat cgccattctc ggcaatacct
     2041 acgccggcca aggcctcgcg tcgggcgtgg ttgctaccca gggcgtttca cccgcagtgg
     2101 ttatcgggcc gaatacaaca ctaacctctc cgatgagaat tggcgtctac acgacagacg
     2161 gcacgcccgc cggaggagat gccgtcgatc cgggcttcag cttcgtcaat cgcggttcaa
     2221 tctcggcgac gccggcgaac gtcgccaact cggcgtcaag cgcgtccacg agcgcaaccg
     2281 ggatagccat gggcggctca tcggtcgttt atccagtcct ggtgggcggg tttttcaatt
     2341 ccggaactct atcggcaggc gtacaaacaa ccggcacggt agctcccgcg gtcagcgcca
     2401 ccgcgctcaa catcggcggc tacacgacgt tcgttgacgc aacctacgac acagcgacca
     2461 attcctggaa caatctgggc acccttacgg gcaagaaaga ctacggtgat tctttggcgg
     2521 cgccagcgct ggcagcgccg gcgctggtga acagcagcgc aacgggcggc ggcacgatct
     2581 cggcgaccgt gagcggctcg cgcggcggca ttgcgacggc aatctctatc gcgtccaccg
     2641 ccaacgtgcc ttcgatcttc aactccggca cgatctctgc ggcggtaagc aactccgacc
     2701 cgacgttgga cggtcaagcg aacagccatc cggtggcggc ttacgccatc gtggatcaat
     2761 cgggcacgct gaactcaatc tacaatgagg gaacgatttc ggccgcggtg tccgcgctgg
     2821 acaatggaag ccaaatcgcc aacgcgatcc tgctcggcaa ccgcgccacc ggcacgcagg
     2881 tgcggatcga caacgtctcc acgcagtcga gaagcgccac gatcgcaggc aacatctcct
     2941 ttggcagcgg cggcacgcaa atcctcaata tcgagggcac cggcacgcac ttggccagcg
     3001 tcacaggcaa cgtcaccttc aacggcacgg gtacgggcgg cgaccagctt accatcggca
     3061 gctatggttc gctcagcggc acgattatcg cccataatgg cgtgtccgtc gatatcgccc
     3121 aaaacggctt acttacgctg gcgaacacca cggctccgct ttatgccacc acgtttgctg
     3181 tccacggcgg cacaaccccc gaaaatggcg gcgcgttggt catcggggtt tccaatacgc
     3241 tgggttcgca aggcgtcatc agcgccaccg gcagcgtgga gatcgaaaga aacgccagac
     3301 taggcttgtc ttttaacagt ttcgtgccgc agggttcccc ggacttcgtc ctgatcacag
     3361 cgcctctcgg ccagttgaat gtggtcgact tggccagcta cagcgacgcg atcggcaaat
     3421 ccaaccagag ctgcaccaat gcgccgacgt ccgactgcgg cggcagcagg ccgttccttt
     3481 tcgcctcggc cgagctgaaa aaagtgagcg acgccgcgag cgacaggctt gttctccagg
     3541 tggtgccgaa gactgcaacc gagttgaatc tcaccggtta cgcgaagcag ctattcccct
     3601 acgccaacac ggcgctcggc gtcgacacca cgctgggcgc ggcgatggtc aatttgatcc
     3661 gcaacgaggc gcaggcgcag gccgcttata attccttcgc cccgaacgtg accggcggca
     3721 gccgcgcgat cgccatctcg atcaccgatc aggcgaccgg cgcggtcggc gcccgcctgc
     3781 gcatgctgaa cctttacggc aagcaagagg gcggcacgac gctgtggagc caggaattct
     3841 tccagatgat caaggacccc ggccagggcg cgctgcaact ggacggcacc aaggaaaaat
     3901 ccggcttcaa ggatcacggc ttcggcttcg cgctcggcat cgacggcggc accccgaaat
     3961 acggctggta tggcgccgcc ctgaccttct acaccggcga cgtcggcgag atcgtgcgca
     4021 attcgcacac caaccaacag tggtacgttt taagcggcta cagcagctgg cgcggcaagg
     4081 gcctgttcct caacaccaag atcgacgccg gctacggcaa ttttgacagc aagcgctaca
     4141 tcaccctgca aaccgatccg aacacgatct attcgcgccg ggcagacagc aagcacgccg
     4201 gcgcactgat ctccggcgtc gtcacgaccg gcgcgctctt ctcctacggc gccaccacct
     4261 tgtcaccgca actgagcgtc gacggcctgc tcatgcgcga agaaggctat accgaaaaga
     4321 atccgggcag cgccaccgtg ggcgacggct tcaacctcaa ggtccagccc tattacgcca
     4381 agtcgctgcg cctgttcctg ggcggcagcg tgcgctacga catcgacctg tgggacttct
     4441 acctgcagcc cgaagcccat accggctacc gctacgatgt cttcaacgat ccggcgaagc
     4501 tgaaagcggc gttcgctcat gccgatttgt cgggcggttc ggcagttccg gggacgcagt
     4561 tcacgctcac cggccccgat ccctcgcaag gcaatttcgt cctcggcggt tcgctcgcga
     4621 ccaccaccga tacctggacg ctgggcgtca acttcgattt cgtgcgcggc agcaacggcg
     4681 ccctccagca ggtcggaacg atcaatctgc tgggacggat ttaagaaaaa ggcgaatagc
     4741 gaatagggag tagcgaatag ggattcgcca ttcgctactc gccatgcgcc atttccacga
     4801 accgctgcac atcctcgtcc ggcgtggcga aagaggtgac caggcgcacc agggtgcgcc
     4861 cgccctccgg cggcccccaa tcgtagaacg aggcgcccag ccgccgcatg cgcgccacgg
     4921 ttgcatccgg caaatagatg aacaccgcat tggcctgtac cggatgggcg agttccgcgc
     4981 cggggaccgc gctcaggccc gccgccagct tccgcgccag cccgttggcg cgcgcggcgt
     5041 gatcgagcca gcgcgtgccc gtga
//
LOCUS       DASWPU010000489         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_96253,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000489 DASWPU010000000
VERSION     DASWPU010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96253"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /gene="gatB"
                     /locus_tag="VGC27_10330"
     CDS             <1..607
                     /gene="gatB"
                     /locus_tag="VGC27_10330"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEY0283005.1"
                     /translation="LPLMLDQAWVDEIAASLPELPDEKRARLMGMGLSAYDATVLVAE
                     KETADYFEAMLAAGADAKAAANWLNNEYFGRLNKAGLAITDGPVSAAANSAIVQMVSA
                     NVISGKTAKDVLDIIWSEGGDPRVLVEERGLKQMTDTGAIETAVDAVIAANPDKVEAA
                     KAKPQLVGWFVGQVMKTTGGKANPAVVNALLKARLGLPESG"
     gene            1391..1663
                     /locus_tag="VGC27_10335"
     CDS             1391..1663
                     /locus_tag="VGC27_10335"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283006.1"
                     /translation="MSCMDIDALTQYENAAKQGRADALYNLGLAYSTGQGVGVDYVAA
                     HKWFNLAALRGSEEAKSWRAQISREMVPAQIAEAQRLAREWLSIFQ"
     gene            complement(1666..>2037)
                     /locus_tag="VGC27_10340"
     CDS             complement(1666..>2037)
                     /locus_tag="VGC27_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0283007.1"
                     /translation="MTELAGYLSDWNNSLILAFAAYNAGPTNAKRWIAAYGDPRGPNV
                     DPIDWIEQIPFSETRNYVQRVIENLEIYRSRLAGRDLPLRILADLYGPNPPPAKVLTY
                     TPPAPVPLPAPKPGSKPSKAK"
BASE COUNT          423 a          560 c          667 g          387 t
ORIGIN      
        1 gctgcccctg atgctggatc aggcatgggt ggacgaaatt gccgcgtcgc tgccggaact
       61 gccggatgaa aagcgcgcgc ggctcatggg gatgggcctg tcggcctacg acgccaccgt
      121 cctcgtcgcc gaaaaggaga ccgcggacta tttcgaggca atgctggcgg cgggtgcgga
      181 cgccaaagcg gcggcgaact ggctgaacaa cgaatatttc ggccgcctca acaaggccgg
      241 actcgccatc acggacggac cggtctctgc cgcggccaac agcgccatcg tgcagatggt
      301 ttccgccaac gtcatctccg gtaagacggc caaggacgtt ctggacatca tttggagcga
      361 aggcggcgat ccgcgcgtcc tcgtggaaga gcgcggcctc aagcagatga ccgacaccgg
      421 cgcgatagag acggcggtgg atgccgtcat cgccgcgaat ccggataagg tcgaggcggc
      481 gaaggcgaag ccgcaactgg tcggctggtt cgtcggtcag gtgatgaaga cgaccggcgg
      541 aaaagcgaat ccggcggtcg tgaacgcgct cctcaaggcg cgactcggat tgcccgaaag
      601 cgggtgattc gcgcatttgc aagcaatcgg cccgtgaatg cgccgttttg cggaaaaatc
      661 gtcagtaaac agacgaagtg caaataacat agtaaaatac gacgttttat gacaggttcg
      721 ccaaaccaca ttttgggggt cggatgagga gtattgcaat ctatcgcgca acccactcat
      781 attgttcgcg ttttgggggg caacgtgagt cgcccgaccc ggacgaacgt aaaaggagtc
      841 ctacctcatg ccaaaagtcg caaagaagaa gaccaagaaa gtcgccaaga agaaggtgaa
      901 gaagacgaag aagaaggcga agaaataggc cttcattttt cggccgcgcc gtttcggcgt
      961 gcgcggtgtt cacggtcgct cccgcttagg cgaattggag taaccgatgg cgaaggcaaa
     1021 ggccgcggtt tccgggcatt ggcgccaagg cgtcgcttgg ggggccgcgg ccagcccgtt
     1081 tctatagtgt catggcccgc gaatgcgggc cacccaggcg atgccagtgt ggctttgtca
     1141 ggccaagctc ggataacagc ccaggtttcg gcgtagagcc tgcccccaac ttgggagccc
     1201 gcattcgcgg gctatgacat tcactttgct gtcgtccgta ctttggccga tcgccttcag
     1261 gcgcatcccg cttattaacg aaacctgaat ttcatgatta agcgtattaa ccgcgcttta
     1321 gcttctttcg ccacaccatc gctgcacaca tggggtgtcc tcatgtgcat tggggaatgg
     1381 gagctgagcg atgtcgtgca tggatatcga cgcgctgacg caatacgaaa acgccgcgaa
     1441 gcaggggcgt gcggacgcgc tgtataattt gggactggct tattccacgg ggcaaggcgt
     1501 cggcgtggat tacgtcgccg cgcacaaatg gttcaatctg gcggcgctca ggggcagcga
     1561 agaggccaag agctggcgcg cgcagatatc tcgcgagatg gtgccggcgc agatcgccga
     1621 ggcgcagcgc ttggcgcggg aatggctatc tattttccag tgaaatcact ttgcctttga
     1681 cggcttgctg ccgggcttgg gcgccggcag ggggaccggc gcaggcggtg tataggtcag
     1741 cactttcgcc ggcggcgggt tcggcccgta gagatcggcc aggatgcgca ggggcaggtc
     1801 gcgcccggca agccggctgc gatagatttc caggttttcg atcacacgct ggacgtagtt
     1861 ccgggtttcg ctgaacggga tctgttcgat ccagtcgatc gggtccacat tcgggccgcg
     1921 cgggtcgcca taggccgcga tccacctttt cgcattggtc ggcccggcat tgtaggcggc
     1981 gaatgccagg atcagcgaat tgttccaatc gctgaggtag ccggcaagtt ccgtcat
//
LOCUS       DASWPU010000490         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_52999,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000490 DASWPU010000000
VERSION     DASWPU010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52999"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VGC27_10345"
     CDS             complement(<1..308)
                     /locus_tag="VGC27_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY0283008.1"
                     /translation="MGETVRLRTGGDIPAYRTGLVAVLDIGSSKVVCLIARAEPGALH
                     VLGAALRESQGLRAGMVTDMALAEESIRDCVAAAENTADARIQNVLISVNCGSPVSVT
                     S"
     gene            complement(308..1249)
                     /locus_tag="VGC27_10350"
     CDS             complement(308..1249)
                     /locus_tag="VGC27_10350"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY0283009.1"
                     /translation="MRPVRQTHKSRDGRGRQERAPARGVSRGAAPAFGRRRQRPDNAV
                     SRLFARLRGFFSFRRPMLAMTLAIIVLTVLAGLFASGVIGRTVHGAGAVADAIIADAG
                     FGIAKLHLSGNTRTAPETILAALGFKPGQSIFTADLPAARARLLRLPWVAEAEVKRRY
                     PDDIGVRIVEKQAFARYLSARGLYVVERTGGLITDKDLEPFQNLPLLNGEGAPKAAAE
                     FVDAVAQHRAIVARVHTYQFRSGRRWNLLLDNGVIVKLPETGWRKQLEALEYLIVDKG
                     ILERDVAEIDLRSPTLYIFVTKSGERKEKKTERGRAI"
     gene            complement(1237..>1841)
                     /locus_tag="VGC27_10355"
     CDS             complement(1237..>1841)
                     /locus_tag="VGC27_10355"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0283010.1"
                     /translation="AAGLPLVKSIVADRRAAAVGHLMEPPYVVKPVNEGSSVGVFIIR
                     KGDNRPPAALGSDQWSFSDEMMVEEFVPGRELTVAVMGTRALAVTEISTNLEFYDYEA
                     KYAPGGSIHTLPAKIPDAVAEEAMRLAERAHTALGCRGVSRCDFRYDDTSGKHRLVVL
                     EVNTQPGMTPTSLVPEQAALTGMSYRALCRWIVEDASCDR"
BASE COUNT          307 a          623 c          609 g          302 t
ORIGIN      
        1 gaggtcacgc tgaccggact gccgcaattg accgagatca gcacgttctg gatgcgggcg
       61 tcggcggtgt tctccgccgc cgcgacgcaa tcgcggatcg attcctcggc cagcgccatg
      121 tcggtcacca tgccggcgcg cagaccctgg ctctcgcgca gcgcggcgcc cagaacgtgc
      181 agcgcgcccg gttcggcgcg ggcgatcagg cagaccactt tcgacgaacc gatgtcgagc
      241 accgccacca gccccgtgcg ataggccggg atgtcgccgc cggtcctgag gcgcaccgtc
      301 tcgcccatca gatcgctctc ccgcgttcgg tcttcttttc tttccgctcg ccggacttgg
      361 tgacgaagat atagagcgtg ggcgacctga gatcgatttc cgccacgtcg cgctccagaa
      421 tgcccttgtc gacgatcaga tattccagcg cttcaagctg tttgcgccag ccggtctcgg
      481 gcagcttcac gatcacccca ttgtcgagca gaaggttcca gcgccgtccc gaccggaatt
      541 gataggtatg gacgcgcgcc acgatggcgc gatgctgcgc caccgcatcg acgaactccg
      601 ccgccgcctt gggcgcaccc tcgccgttga gcaaaggcag gttctggaac ggttcgaggt
      661 ccttgtcggt gatgagaccg cccgtgcgct ccaccacata aagccccctg gccgacaggt
      721 agcgcgcgaa agcctgcttc tccacgatcc gcacgccgat gtcgtcggga taacggcgct
      781 tgacctcggc ctcggcgacc cagggaagcc tgagcagcct ggcgcgcgcg gccggcagat
      841 cggcggtgaa aatcgactgg cccggcttaa agcccaaagc ggccaggatc gtctccggcg
      901 ccgtgcgggt attgccggaa agatgcagct tggcaattcc gaaccccgca tcggcgatga
      961 tcgcatcggc gaccgccccc gcgccatgaa cggtgcgtcc gatcacgccg ctggcgaaca
     1021 ggccggcaag cacggtcaag acgatgatgg ccaaggtcat cgccagcatc gggcggcgga
     1081 aagagaagaa cccgcgcaag cgcgcgaaca ggcgcgagac cgcattgtcg ggtctttgcc
     1141 ggcggcggcc gaacgccggc gccgcgccgc ggctgacgcc acgggcgggc gcccgctcct
     1201 gccgaccgcg tccgtcgcgg ctcttgtgcg tctgcctcac cggtcgcacg aagcatcctc
     1261 cacgatccag cggcacagcg cgcggtagct catgccggtc agggcggcct gttcgggaac
     1321 cagagaggtc ggtgtcatgc cgggctgggt gttgacctcg agcacgacca ggcgatgctt
     1381 gccggacgta tcgtcgtagc ggaaatcgca gcgcgaaacg ccgcggcaac ccagtgccgt
     1441 atgggcgcgt tcggccagcc gcatcgcctc ttcggcgacg gcgtcgggaa tcttcgccgg
     1501 caatgtgtgg atggagccgc cgggagcgta tttcgcttcg taatcgtaga attcgagatt
     1561 ggtgctgatt tcggtgacgg cgagcgcgcg cgttcccatc accgcgacgg tcaattcgcg
     1621 gcccggcacg aattcctcga ccatcatctc gtcgctgaag ctccattggt cgctgcccag
     1681 cgcggcgggc gggcggttgt cgcccttgcg gatgatgaag acgccgacgg acgagccttc
     1741 gttcaccggc ttcaccacat agggcggctc catcaggtgg cccacagcgg cggcgcggcg
     1801 gtcggcgacg atggatttga ccagcggcag gccggcggcg c
//
LOCUS       DASWPU010000491         6921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_22981,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000491 DASWPU010000000
VERSION     DASWPU010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22981"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1153)
                     /gene="rseP"
                     /locus_tag="VGC27_10360"
     CDS             complement(23..1153)
                     /gene="rseP"
                     /locus_tag="VGC27_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY0283011.1"
                     /translation="MSVLHDLIAWAPVGLPAFIFLITFVVFFHELGHFLVARAFGVGI
                     ETFSIGFGREIFGWTDGKGTRWKISWLPLGGYVKFLGDENAASTPDRERIATMDAAER
                     SNAFPLKPLYQRALVVVAGPVANFVLAIIVFTLLYTFVGAKALSTVVGSVTPDSPAAA
                     AGIKPGDKITAVNGKPVKLFYGELQGIVQASKGKPLAFSIERDGRPMLFKITPREITV
                     PNIYGGSEKAMAIGVGPAQGAPGNTVFIPVPTMTAPVIAVSQTWFIVDASLTSVWRMV
                     SHQANANQLTGVVGIAGISQKAASHGFYDLIYLVAFISVSIGLINLFPIPLLDGGHLL
                     YYGCEVVLGRPLGERAQDVGFRVGLALVLGLMIFATWNDLVR"
     gene            complement(1182..2420)
                     /gene="dxr"
                     /locus_tag="VGC27_10365"
     CDS             complement(1182..2420)
                     /gene="dxr"
                     /locus_tag="VGC27_10365"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY0283012.1"
                     /translation="MNALAAHRRHVDMPDLKPVLARRVTILGSTGSIGVSTLDVIGHA
                     RQLYGADVLPVEALTAQSNVDALAAQARKINPKIAVIGDETLYFRLKDALAGTGIEVA
                     AGRGAVIAAAARPSEVVMVAIMGAAALEPALAAVARGATVALANKECVVAAGTVFHRA
                     VAKSKAAVIPVDSEHNAIFQVFRAADADEVDRVTLTASGGPFRDWTLERMLAATPEQA
                     VAHPNWSMGAKISVDSATLMNKGLELIEAHFLFALPAEKLAVVVHPQSIVHCLVSYVD
                     GSTLAHLSAPDMRTPIAHALAWPRRISSPSRKLDLPSVAQLTFQAPDHQRFRCLALAL
                     DCLNRGGVSPTILNAANEIAVDAFLSRRIGFLDIPRVVEATLDESPDANVEAESLEGV
                     LAADALARELAGNICRRMMT"
     gene            complement(2417..3289)
                     /locus_tag="VGC27_10370"
     CDS             complement(2417..3289)
                     /locus_tag="VGC27_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY0283013.1"
                     /translation="MSKPRNSITANRPPVSLRALKFSRDWVTRPFFGVALALIVIAAI
                     YGGTPYLAIVVAVAVFAAAREWHRIVGEGGVAPELAVTTVSIWLMLIAFVAWPRSITP
                     YVMLLCGTFLVLALSMTRRSRPLWRAGGVLYLGIPSLAILALRDIPPHGAWLIGALFA
                     VVWAADTGALIVGNLVGGPRLAPRLSPNKTWSGTIGGMVAAAIAEAIFIAIIGGHAGA
                     AALFGAGLAVFAHLGDLFESWVKRRFHTKDSGSLIPGHGGVLDRIDSTLFAVVALEIA
                     VFVFHLDPLFGARP"
     gene            complement(3286..4020)
                     /locus_tag="VGC27_10375"
     CDS             complement(3286..4020)
                     /locus_tag="VGC27_10375"
                     /inference="COORDINATES: protein motif:HMM:NF011408.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY0283014.1"
                     /translation="MAAAPSNSNLPRHVAIIMDGNGRWAQKRHLPRQAGHVAGVNAVR
                     EVVRQACDSGIENLTLFAFSSENWKRPPKEVGALMGLFRLYFRNDLEELVARGARVRI
                     IGSRCRVDDDIRQMIDDAERRTLGNSGINLTFAFDYGGQEEIAAAARELARAAAEGRL
                     DPETITPELVSSRLFTSSLPEPDLVIRTSGERRLSNFLLWQSAYAELMFVETLWPDFG
                     RREFLAALEEFSQRERRFGALPESAA"
     gene            complement(4121..4681)
                     /gene="frr"
                     /locus_tag="VGC27_10380"
     CDS             complement(4121..4681)
                     /gene="frr"
                     /locus_tag="VGC27_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY0283015.1"
                     /translation="MAETYNKDEMTRRMNGAVAGLKGEFAGLRTGRASPALLDPVSIE
                     AYGNQMHVSQLGTISTPEPRLLTVQIWDRSMVKAVDKAIRDAGLGLNPQVDGQILRIP
                     IPELNEERRKELVKLAHKYAEQGRIAVRNVRRDGMEILKKLEKDHKISQDEHHKLGDE
                     LQKITDAHIKEVDLLLQGKEQEIMQV"
     gene            complement(4691..5461)
                     /gene="pyrH"
                     /locus_tag="VGC27_10385"
     CDS             complement(4691..5461)
                     /gene="pyrH"
                     /locus_tag="VGC27_10385"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY0283016.1"
                     /translation="MLSLSKREGGNSEAKMLSTPKYRRVLLKLSGEALMGSQPFGIDT
                     VTVDRIAADAKQAVDTGIQLCIVIGGGNIFRGLSGAAKGIERATADYMGMLATVMNAL
                     AMQAALERGGVASRVLSAIPMSTVCEPYIRRRAIRHLEKGRVVIFAGGTGNPFFTTDT
                     AAALRAAEMNCEALLKGTQVDGVYSADPKKVAGAKRYAALTYQEVLARDLRVMDASAV
                     SLSRENGIPIIVFSIHDKGALVAVLKGEGRATIIQDRA"
     gene            complement(5520..6440)
                     /gene="tsf"
                     /locus_tag="VGC27_10390"
     CDS             complement(5520..6440)
                     /gene="tsf"
                     /locus_tag="VGC27_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY0283017.1"
                     /translation="MANITASLVKDLRDKTGAGMMDCKRALNETGGDMEAASDWLRKT
                     GLLKAAKKATRVAAEGLIGVAVGGDAGALVEVNSETDFVARNDEFKEFVKTAAKLALE
                     EGGDVEKLLAKPLGGGSVKDALTNMIAKIGENMSVRRAAALAVSPGVVSSYVHNAASP
                     ELGKIGVLVALKSTADKAKLAALGKQLAMHVAAAAPLALAPEHLSKEVVDRERAIQAD
                     LARQSGKPENLIEKVLEGRMRKFFEDTVLLSQTFVIDGETQIAKVIEKASKDFGAPVE
                     IAGFVRFQVGEGIDKISGDFADEVGKLAGR"
     gene            complement(6462..>6921)
                     /gene="rpsB"
                     /locus_tag="VGC27_10395"
     CDS             complement(6462..>6921)
                     /gene="rpsB"
                     /locus_tag="VGC27_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY0283018.1"
                     /translation="EETLGSAQGSGLTKKELLGLLRERDKLERSLGGIKEMGGLPNML
                     FVIDTNKEAIAISEAKKLGIPVTAILDSNCDPDGIAHPIPGNDDAARAIALYCDLIAR
                     AVIDGLSEGQLAAGVDTGEAAVLPDLAPAADVPAAELVAADAPIEDKPTA"
BASE COUNT         1253 a         2292 c         2156 g         1220 t
ORIGIN      
        1 ggccgaggtt tcgcaaaggg ttctaccgga ccaagtcatt ccacgtggcg aagatcatca
       61 gaccgagcac caaagcaagc ccaaccctga atccaacgtc ctgggcccgc tcacccagcg
      121 gccgccccaa cacaacctcg catccatagt acagaagatg gcccccgtcg aggagaggaa
      181 tgggaaaaag gttgattaga ccgatgctta cagaaatgaa agccaccaga tagataagat
      241 cgtagaagcc gtgcgaagcg gctttttggg atatcccggc tattcccact accccggtca
      301 actggttcgc gttagcctgg tgcgagacca tccgccacac agaggtgagc gaggcatcga
      361 cgatgaacca ggtctgggat acggcgatga ccggcgccgt catggtcgga acgggaataa
      421 agacggtgtt cccgggcgcg ccctgggcgg ggccgactcc gatggccatg gccttctcgg
      481 acccgccgta aatgttcggg acggtaatct cgcggggcgt gatcttgaac agcatcggcc
      541 ggccgtcgcg ctcgatcgaa aacgcgagcg gcttgccctt gctggcttgg acgataccct
      601 gaagttctcc gtagaacagc ttgaccggtt taccgttgac ggccgtgatc ttgtcgccgg
      661 gtttgatgcc ggccgcggcg gccggcgaat ccggcgtcac cgagccgacg acggtggaaa
      721 gcgccttcgc tccgacgaag gtgtagagaa gcgtgaagac gatgatggcc agaacgaaat
      781 tcgccaccgg tccggcgaca acaaccagcg cgcgctgata gagcggcttc aggggaaagg
      841 cgttcgagcg ctcggcggcg tccatggtgg cgatgcgttc gcggtccggc gtgctggcgg
      901 cgttctcgtc gccgaggaat ttcacatagc cgccgagcgg caaccaagag atcttccaac
      961 gcgtgccctt gccgtcggtc cagccgaaaa tttcccggcc gaagccgatg gagaacgtct
     1021 cgatgcccac gccgaaggcg cgggccacca gaaaatggcc aagctcgtga aagaacacca
     1081 caaaggtgat gagaaaaata aacgcaggca gtcctaccgg ggcccaggcg atcaaatcgt
     1141 gcaatacgga cactatcact actcctgtat gccccaccac ttcacgtcat catgcgccgg
     1201 catatgtttc cggcaagttc ccgcgccagg gcatcggccg ccagcacgcc ttcaagggat
     1261 tcggcctcga cgttcgcatc gggactttcg tccagggtcg cctccaccac gcgcggaatg
     1321 tccagaaaac cgattcgccg gctcaggaag gcgtccacgg cgatttcgtt ggccgcgttc
     1381 aggatcgtag gcgacacccc gccccggttc aagcagtcca gcgccagcgc caaacagcgg
     1441 aatcgctggt gatccggggc ctggaaggtc aattgcgcga cagaaggcaa gtcgagcttg
     1501 cgcgacggcg aggaaatgcg ccggggccag gcgagcgcgt gggcgatggg cgtgcgcatg
     1561 tcgggcgccg acaaatgggc cagggtcgag ccgtccacgt aggacaccag gcaatgcacg
     1621 atcgactggg gatgcaccac gacggcaagc ttttccgccg gcagggcgaa caggaaatgc
     1681 gcctcgatga gttccagtcc cttgttcatc agcgtggcgc tgtcgacgga gatcttcgct
     1741 cccatcgacc agttgggatg cgccacggcc tgttcgggcg tcgccgcaag catgcgctcc
     1801 agcgtccagt cgcgaaacgg cccgcccgaa gcggtgagcg tcacgcggtc aacctcgtcc
     1861 gcatcggccg cccggaagac ctgaaaaatc gcattatgtt cggaatcgac cggaatcacc
     1921 gcggccttcg atttggcgac cgcgcggtga aacacggttc ccgcggccac cacgcattcc
     1981 ttgttggcga gcgccaccgt cgccccgcgt gcgaccgcgg ccagcgccgg ctccaaggcc
     2041 gccgcaccca tgatcgccac catcacgacc tcggaaggcc tggcggcggc cgcgatcacc
     2101 gccccgcgtc cggcggcaac ctcgatgccc gtccccgcca acgcgtcctt gagcctgaaa
     2161 tacagggttt cgtcgccgat cacggcgatc ttcggattga tcttccgtgc ctgggcggca
     2221 agcgcatcga cattcgattg cgccgtcagc gcttccaccg gcagcacatc ggcgccgtaa
     2281 agctggcgcg catggccgat cacgtcgagc gtgctgacgc cgatggaccc ggtggaaccg
     2341 aggatggtga cgcgccgcgc cagaaccggc ttaaggtccg gcatgtccac atgacggcgg
     2401 tgggctgcaa gcgcgttcac ggtcttgctc caaacagcgg gtcgaggtga aagacgaaga
     2461 ccgcgatctc caacgccacg accgcgaaaa gcgtggaatc gatgcggtcg agaacgccgc
     2521 catggccggg aatcagcgag ccgctgtcct tggtgtggaa ccgccgcttc acccaggatt
     2581 cgaacaggtc gccgagatgg gcaaaaacgg ccagccccgc tccgaacaag gcggccgcgc
     2641 cggcatggcc gccgatgatg gcgatgaaga tcgcttccgc gatcgccgcc gcgaccatcc
     2701 cgccgatcgt tccggaccag gtcttgttcg gcgacagccg cggcgcgagc ctcggcccgc
     2761 caaccagatt gccgacgatc agcgcgcccg tgtcggccgc ccacacgacg gcgaacaggg
     2821 cgccgatcag ccacgcgcca tgcgggggta tgtccctcaa cgccaggatc gccagcgaag
     2881 gaatgcccag ataaagaact ccgccggccc gccacagcgg acggctccgc ctggtcatgc
     2941 tcaacgccag caccaggaaa gtgccgcaga gcagcatgac ataaggcgtg atcgagcgcg
     3001 gccaggccac gaacgcgatc agcataagcc agatcgaaac cgtcgtgacc gcaagttccg
     3061 gcgccacgcc gccctcgccc acgatccgat gccattcgcg cgcggcggcg aagacggcga
     3121 ccgcaacgac gatcgccaga taaggcgtgc cgccgtaaat cgcggcgatc acgatgagcg
     3181 ccagcgccac cccgaagaac ggcctggtga cccagtcgcg cgaaaactta agggcacgca
     3241 ggctcaccgg cggccggttg gcggtgatgc tgttacgcgg cttgctcacg ctgcggattc
     3301 cggcaaggcg ccgaaccggc gctcgcgctg cgagaattcc tcgagcgcgg cgagaaactc
     3361 ccgccggccg aaatccggcc acaacgtctc cacgaacatc agttcggcat aagccgactg
     3421 ccacagcagg aaattgctca agcgccgctc gccgctggta cggatgacga gatccggttc
     3481 gggcagagag ctggtgaaca ggcgcgagct gaccagctcc ggcgttatcg tttcgggatc
     3541 gagcctgccc tctgccgcgg cgcgcgccag ttcccgcgcg gcggcggcga tttcctcctg
     3601 tccgccgtaa tcgaaggcaa aggtgaggtt gatgccgcta ttgcccaagg tgcgccgctc
     3661 ggcatcgtcg atcatctggc ggatgtcgtc gtccacgcgg cagcggctgc cgatgatgcg
     3721 cacgcgcgcg ccgcgcgcga ccagttcctc caggtcgttg cgaaaataga ggcggaacaa
     3781 ccccatcagc gctcccacct ccttgggcgg ccgtttccag ttctccgagg agaaggcaaa
     3841 cagggtcagg ttctcgatac cggaatcgca agcctggcgc acgacctcgc gcaccgcatt
     3901 gacgccggcg acatgacccg cctgccgggg aaggtgccgc ttctgcgccc agcgtccgtt
     3961 gccgtccatg atgatggcga catggcgcgg aaggttgctg ttgctcggcg cggcggccat
     4021 acgagtatcc cgacagcccc gccgtccgtt ggtatcgggc gcgggagttg attgcgaatg
     4081 ccctatgctg accttctcaa ggcgccaggg caacgttcct tcagacctgc atgatctcct
     4141 gttctttgcc ttggagcagc agatcgacct cctttatgtg cgcgtcggtg atcttctgca
     4201 attcatcgcc gagcttatga tgctcgtctt ggctgatctt gtggtctttt tcgagctttt
     4261 tgaggatttc catgccgtcg cggcgcacat tgcggaccgc tatgcgcccc tgttcggcat
     4321 atttatgggc caatttgacc agttccttgc gccgctcctc gttcaattcc ggaataggaa
     4381 tgcgcaaaat ctggccgtcc acctggggat tgaggccgag tccggcgtca cggatcgcct
     4441 tgtccacggc cttaaccatg ctgcggtccc atatctgcac ggtcagcagc cggggttcgg
     4501 gggtggagat ggtgccaagc tggctgacgt gcatctggtt gccgtaggcc tcgatcgaaa
     4561 cgggatcgag caaggccggg ctggcgcgcc cggtgcgcaa gccggcgaac tcccccttca
     4621 gccccgctac ggcgccgttc attcggcggg tcatttcatc cttgttgtag gtctcggcca
     4681 tctacgtctc ctatgccctg tcttggatga tcgtcgcccg tccctcgcct ttcagcaccg
     4741 caacgagcgc gcctttgtcg tgaatcgaaa atacgatgat gggaatcccg ttttcacgcg
     4801 aaaggctgac ggcggaagcg tccatgaccc tgagatcgcg cgccaggacc tcctgatagg
     4861 tcagcgctgc ataccgtttc gcgccggcga ccttcttcgg atcggcactg taaaccccgt
     4921 cgacctgcgt acccttgagc agggcttcgc agttcatctc ggccgccctg agcgccgccg
     4981 ccgtgtcggt ggtgaagaac ggattgccgg tgccgcccgc gaagatcacc acccggccct
     5041 tttccaggtg gcggatggcg cggcggcgga tatagggttc gcagaccgtg ctcatcggga
     5101 tggcggagag cacgcggctc gccacgccgc cgcgttcgag cgccgcctgc atcgccagcg
     5161 cgttcatcac ggtcgccagc atgcccatat agtcggcggt cgcccgctcg atgcccttgg
     5221 ccgcgccgga cagcccgcga aagatgttgc cgccgccgat cacgatgcag agctgaatac
     5281 cggtatccac cgcctgcttt gcatcggcgg cgatgcgatc gaccgtgacc gtgtcgatgc
     5341 cgaagggctg cgaccccatc aacgcctcgc cggaaagctt cagaagcacc cggcggtatt
     5401 tcggcgttga tagcattttt gcctcggagt tgccgccctc acgcttcgac aagctcagca
     5461 tgagggctct tgactaaacc ctcaccctga gcccgtcgaa gggtgggggc aacggaaacc
     5521 tagcggcccg cgagttttcc cacttcgtcc gcgaagtcgc cggatatctt atcgatgcct
     5581 tcgccgacct ggaagcgcac gaagccggcg atctcgaccg gcgcaccgaa atccttggag
     5641 gccttctcga tcaccttggc gatctgggtc tcgccgtcga tcacgaacgt ctgcgacagc
     5701 agcacggtgt cctcgaagaa cttccgcatc cggccttcca gcaccttctc gatgaggttt
     5761 tcgggcttgc cggactggcg tgcaagatcg gcctgaatgg cgcgctcgcg atccacaact
     5821 tctttcgaca gatgctcggg agcgagggcg agcggggccg ccgccgccac gtgcatggca
     5881 agctgcttgc cgagagcggc cagcttggcc ttgtcggcgg tcgatttcag cgccaccagg
     5941 acgccgatct tgccgagttc cggcgaggcg gcgttatgca cataggacga caccacgccg
     6001 ggcgacaccg ccagcgcggc ggcgcgacgc acgctcatat tctcgccgat cttggcgatc
     6061 atattggtca gggcgtcttt cacgctgccg ccgcccagcg gcttggccag cagcttctcg
     6121 acgtcgccgc cttcttccag cgccagcttg gcggcggtct tgacgaactc cttgaactcg
     6181 tcgttgcgcg cgacgaagtc ggtctcggag ttcacttcca ccagggcgcc cgcgtcgccg
     6241 ccgacggcca cgccgatcag cccttccgcg gccacccgcg tggccttctt tgcggctttc
     6301 aacaggccgg tcttgcgcag ccagtcgctc gcggcttcca tgtcgccgcc ggtctcgttg
     6361 agcgctctct tgcagtccat catgcccgcg ccggtcttgt cgcgcaggtc cttcaccagc
     6421 gatgcggtga tgttcgccat tcttgcctcc gttgtcgcgt atcaggcggt gggcttgtct
     6481 tcgatcggcg cgtctgccgc caccagctct gcggccggta cgtccgcggc cggggccaga
     6541 tccggcaaca ccgccgcttc gcccgtgtcc acacccgccg cgagctggcc ttcggacagt
     6601 ccgtcgatga ccgcgcgcgc aatcaggtcg caatagagcg caatggcgcg ggccgcgtcg
     6661 tcgttgccgg gaatcggatg agcgatgccg tccggatcgc agttggaatc gaggatcgcc
     6721 gtcaccggga tgccgagctt cttggcttcc gaaatggcga tggcttcctt gttggtgtcg
     6781 atcacgaaca gcatgttggg caggccgccc atttccttga tgccgccgag cgagcgctca
     6841 agcttgtcgc gttcgcgcag caggccgagc agttccttct tggtcaggcc ggagccttgg
     6901 gccgaaccca gcgtctcctc g
//
LOCUS       DASWPU010000492         4591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_9777, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000492 DASWPU010000000
VERSION     DASWPU010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9777"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VGC27_10400"
     CDS             complement(<1..958)
                     /locus_tag="VGC27_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0283019.1"
                     /translation="MTATSGTVLDLPAVGRLFGDAATARGWDFLGNVPVSLRHRVRDG
                     VAELVAQHGETLKCCFPMGQGGDGPFERLRRIRSFDDYPGMVVSSDHGNVFNRRFYDR
                     HVATGGFVGCQPGGIAPVFSEAELVDPKGWIGTFAVAPFVLLIDHRKLNGLPVPRRWA
                     DLLDPVYRGQVVFSGWRRDGEQHYRQYNKFFLLSIAKEFGLEGVARLIRNVPALIHSA
                     QMPRLAGGNASPGGIYVVPWSLADICPRRDRTEVVWPTDGALAYPLWLTVKAARRNRL
                     GALIDYFYGEKLARYLDQNRYPALLPGREHGLPRAAQLKWLGW"
     gene            complement(964..1989)
                     /locus_tag="VGC27_10405"
     CDS             complement(964..1989)
                     /locus_tag="VGC27_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283020.1"
                     /translation="MPVSSPPDWLIRPPPAPTTRILFLARHAPLAPAYLPQSYAGDGG
                     YPAYYHRVWQALTELGYPVTTANDGLALLAAAGQVDLVFSLYNRMPIRNPEIFATALC
                     SFLHLPIVGSAPNTRALAEDKWFSKLLARSIGIPVSDGAVYAAAEELAVPPPFAGPYF
                     VKNRFGAASEGIGPDSIQDDWTAAAQIAETLLKRGMSVLVENYVPGIDITVPVLGGKQ
                     PIMLGVVQPGSDQRGGIITEDLKRDDPMGYELFEAGAAETPLRTDVAALWRAAGPMDY
                     FRLDYRFDPQGGRRVFLEFNICCHIGRSGAFCLAASQWGLSQADVLGHVIEYSLRRQA
                     DTRGAGG"
     gene            2248..3483
                     /locus_tag="VGC27_10410"
     CDS             2248..3483
                     /locus_tag="VGC27_10410"
                     /inference="COORDINATES: protein motif:HMM:NF022115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HEY0283021.1"
                     /translation="MDMGLASLDRTSTVQKAPAAEAAWIRPGVVVHQSAETVDRLLEA
                     FAFKLRQRGFNVAGYVQRSNSGCTGKGLGCAPNIEYLDLSTNQTVSVERAAATAYLRR
                     AMRDNADLLVISRFAGSIASAAWARPGGNNSAQGLPLLTSIAGQCIHKWFDYARQDGA
                     MISPDLKSLWAWWGAERLYRDLALGVAEHEVCRIACGTRWIMVEGPFGAGLAYLPRSP
                     RELLPQLPHYAGLSLRELAALSQSWDPLKMALGIAAINAHYNRYDLDAASGNGVRTFR
                     GAAGRVVVIGAFPGIDGILPHCAVIEADPRPGEYPAAAMDTLLPVCGGAIVNASALIN
                     RSLPRILRLASNRPVALIGPATPMTPRLHDYGLSLLGGFIVRDANGLAAAIRAGVLPK
                     DFGRFGRYVHLSCGEPAAS"
     gene            complement(3491..3976)
                     /locus_tag="VGC27_10415"
     CDS             complement(3491..3976)
                     /locus_tag="VGC27_10415"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEY0283022.1"
                     /translation="MDAPHLRIAVASDSLIHLDANFAAAKQMVFYDVARGASEFVDVV
                     HFRRGAGKGPGGGAGPANGCCMTDDMQDDGGAGRDPTTERIEALKGCSVLFTLGISEV
                     AAVRVHNMRIFPVASERARTIDDVIANLQRMMNGAPPLWLRRVMRDGNGNRLGLDDQE
                     I"
     gene            complement(4034..>4591)
                     /locus_tag="VGC27_10420"
     CDS             complement(4034..>4591)
                     /locus_tag="VGC27_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEY0283023.1"
                     /translation="QKISEVTGKPIPESLVKERGIAINALQDLAHMFFADKKVALYGH
                     PDLVIGLTEFCLEVELKPVLLLLGDDNGKYKRDPRILALKEKVDWDMEIVCNADLWEL
                     EKRAKDPDYQIDLILGHSKGRYVAIDNNIPMVRVGFPTFDRAGLYRRPTMGYQGAIEL
                     AENIANALFAHMEYTKDREWLLNTW"
BASE COUNT          908 a         1428 c         1413 g          842 t
ORIGIN      
        1 cccaacccag ccatttcagc tgggcggcgc gcggcaagcc gtgttcgcgg cctggaagga
       61 gcgcgggata gcggttctga tcgagatagc gcgcgagttt ttcaccgtaa aaatagtcga
      121 tcaaagcgcc caggcggttg cgccgtgcgg ccttgaccgt cagccacaag ggataggcca
      181 aggcgccgtc cgtcggccac acgacttcgg tacgatcacg acggggacag atatcggcca
      241 gcgaccaggg cacgacataa atcccgcccg gcgatgcatt cccgcccgcc agccggggca
      301 tttgggcgga atggatcagc gccggcacgt tgcggatcag gcgcgccacg ccttcgagac
      361 cgaattcctt ggcgatgctg aggagaaaaa acttgttgta ttggcgataa tgctgctcgc
      421 cgtcgcggcg ccagccgctg aacaccacct gtccacgata gaccgggtcg agcagatcgg
      481 cccagcgccg cggcaccggt aagccgttca gcttgcgatg atcgatcagc aacacaaagg
      541 gtgccaccgc aaaggtgcca atccatcctt tcggatcgac cagttcggcc tcgctaaaga
      601 ccggcgcaat gccgccgggc tgacagccga cgaagccgcc ggttgcaacg tggcgatcat
      661 agaagcgtcg gttgaagaca ttgccgtgat cggacgaaac caccatgccc ggataatcat
      721 cgaagctacg aatccggcgc agccgttcga agggaccgtc gcccccctgc cccatcggaa
      781 agcagcattt cagggtttcg ccatgctgcg ccacaagctc ggcaacgccg tcgcggacac
      841 ggtgccgcag ggaaaccgga acattgccga gaaagtccca gccccgcgcg gtggcggcat
      901 cgccaaacag ccggccaacc gcaggaaggt cgagaacggt tccggatgtc gcggtcaaga
      961 cattcatccg cctgctccgc gcgtatcggc ctgccggcgc agactatatt cgatgacatg
     1021 gccgaggacg tcggcttgcg acagccccca ctgggacgcc gccagacaaa aggcgccgct
     1081 gcgcccgata tggcagcaga tattgaattc gagaaaaacc cggcgtccgc cttgaggatc
     1141 gaagcgataa tcgaggcgga aataatccat cgggcccgcc gcccgccaca acgccgcgac
     1201 atccgtgcgc aggggcgttt cggcggcgcc cgcttcgaac agttcatacc ccatggggtc
     1261 atcgcgcttg agatcttcgg tgatgatccc gccgcgctga tcggagccag gctgcaccac
     1321 ccccagcatg atcggctgct tcccgccaag caccggaacg gtgatgtcga tgcccggaac
     1381 atagttctcg accagcaccg acatgccgcg tttcagcagg gtttcggcaa tttgcgcggc
     1441 agcggtccaa tcgtcctgaa tggaatccgg accaatccct tccgaagcgg ccccgaaacg
     1501 gttcttgacg aaatacggtc cggcaaaagg aggcggcacg gccaattcct ccgccgccgc
     1561 ataaaccgcc ccatccgaca ccggaatgcc gatcgatcgg gccagcaatt tggagaacca
     1621 tttgtcttcg gccaaagcgc gggtattggg cgcagagccg acgatgggaa ggtgaaggaa
     1681 cgaacaaagt gcggtggcaa aaatctccgg attgcggatc ggcatccggt tgtagagcga
     1741 gaaaaccaga tcgacctgac cagccgccgc caaaagcgca aggccgtcat tcgctgtggt
     1801 aacggggtag ccaagctcgg tcaaagcctg ccacacccga tggtaatagg ccggataacc
     1861 gccatcaccg gcataggatt ggggaagata agccggcgcc agcggcgcat ggcgggcaag
     1921 gaacaggatg cgggttgtcg gtgccggcgg cgggcgtata agccaatcgg ggggcgacga
     1981 aaccggcatt gcaggacacg ctttcaagaa aactatttgt cgacagtatg ctgatttcct
     2041 aagcaagcgt cgagccaacg gcggaaaact ccgttcgcgg gccgctttct cgcggaattg
     2101 tacgtcgatg taggaaattg aagactcatt tgttcgcgtt tgtacgcatt ggcaggatga
     2161 accgctattt cccctgtccg atcatgattc aggccgctgg cccatggctt gcttgttgcc
     2221 tggtgtgggc agcaagattg ggtcgtcatg gatatgggac tagcgtccct ggaccgcacc
     2281 tctaccgtac agaaggcccc agcggcggag gcggcgtgga tcagaccggg cgtcgttgta
     2341 caccagtcgg ccgaaaccgt ggaccggttg ctggaggcat ttgccttcaa gctgcgtcag
     2401 cgcgggttca acgtggccgg ttatgtccag cgcagcaata gcggttgtac cggcaaaggt
     2461 ctgggttgtg cccccaacat cgaatatctg gacctgagca cgaaccaaac ggtatcggtg
     2521 gaacgcgccg cggcaaccgc ctatttgcgc cgggcgatgc gcgataacgc cgatttgctg
     2581 gtcatcagcc gcttcgccgg ttccatcgcg tccgccgcat gggccaggcc gggcggcaac
     2641 aacagcgcgc aaggactgcc gctgctgacc tcgatcgcgg gtcaatgcat ccataaatgg
     2701 ttcgattacg cgcgccagga tggggcgatg atctcgcccg atttgaagtc gctttgggca
     2761 tggtggggag cggaacggct ttaccgcgat ctggcgctcg gcgtcgccga gcatgaggta
     2821 tgccgcatcg cctgcggcac ccgctggatc atggtcgaag ggccgtttgg cgccggattg
     2881 gcgtatctgc cgcgctcgcc gcgcgagctg ctgcctcagt tgccgcatta tgccggcctg
     2941 agtttgcgag agcttgcggc gttgtcgcaa tcctgggatc cgctcaagat ggcgcttggc
     3001 atcgccgcga tcaatgccca ttacaaccgc tacgatttgg atgccgcctc gggcaacggc
     3061 gtcagaacgt ttcgcggcgc cgccgggcgc gtggtggtga tcggcgcgtt tcccggtatc
     3121 gacggcatcc tgcctcattg cgccgtgatc gaagccgatc cgcgccccgg cgaatatccc
     3181 gcggcggcga tggatacgct tttgcctgta tgcggcggcg ccatcgtcaa tgcctcggcg
     3241 ctgatcaatc gcagcctgcc gcgcattttg cggctggcct cgaaccgccc cgtggcgttg
     3301 atcggaccgg caacgccgat gacgccccgg ctgcatgatt acggcttgag cctgcttggc
     3361 ggatttatcg tccgcgacgc caacggtctt gccgccgcca tacgcgccgg cgtcttgccg
     3421 aaagacttcg gacgcttcgg acgctatgtc cacttgtcat gcggcgaacc ggcggcttcg
     3481 tgaccgcggc tcatatctcc tgatcgtcga gccccagccg gttgccattg ccgtcgcgca
     3541 tgacccggcg cagccacaag ggcggagcgc cattcatcat ccgctgcaga ttggcgatga
     3601 catcgtcgat ggtgcgggcg cgctcgcttg ccaccgggaa tatccgcatg ttgtggaccc
     3661 ggacggcggc cacctcgctg atgcccagag tgaacagcac ggagcagcct ttcagcgctt
     3721 cgatccgctc cgtggtagga tcgcggccgg cgccgccgtc atcctgcata tcgtccgtca
     3781 tgcagcagcc gtttgccggc cccgcaccgc cgcccggacc ttttccggcg ccacggcgaa
     3841 aatgcaccac atcgacgaat tcggaagcgc cgcgggcgac gtcgtagaac accatctgct
     3901 tggcggcggc gaaattggcg tccagatgta tcaggctgtc ggaagcaacg gcgattctca
     3961 aatgcggcgc atccatggcg ttatcccgtt atgaactgcg atatatctgc gccgccgccg
     4021 gccggcgagg cgctcaccac gtattcaaga gccattcgcg gtctttggta tattccatat
     4081 gcgcgaacag ggcgttggcg atgttctcgg ccagctctat agcgccctga tagcccatcg
     4141 tgggacggcg atagagcccg gcgcggtcga aggtcggaaa accgacccga accatcggga
     4201 tattattgtc gatcgccaca taacgtccct tggaatggcc cagaatcaaa tcgatctgat
     4261 agtccgggtc cttggcccgc ttttcgagtt cccataaatc ggcgttgcag acgatttcca
     4321 tgtcccaatc gaccttctcc ttcaacgcaa ggatgcgcgg atcgcgctta tacttgccgt
     4381 tgtcatcgcc cagaagcagc agaaccggct tcaactccac ctcgagacag aactcggtca
     4441 aaccgatcac caggtcggga tgaccgtaca aggcgacctt tttgtcggcg aagaacatat
     4501 gggccaggtc ctgcagcgca ttgatggcga tgccgcgttc cttgaccagg gattccggga
     4561 tgggcttgcc ggtgacctcg ctgatcttct g
//
LOCUS       DASWPU010000493         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_96295,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000493 DASWPU010000000
VERSION     DASWPU010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96295"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1968)
                     /locus_tag="VGC27_10425"
     CDS             complement(52..1968)
                     /locus_tag="VGC27_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0283024.1"
                     /translation="MSIAAKFRDLSVSAKVVSAFTCVLIATIALGIFSVQRLSQVDAN
                     AVEIRDHWLKSTRVLGEVQYAATRFRSWNGAVLELSTPEQQAMGKKNLDLLEVAVGEA
                     LRKYQAMANSDKERAFAEQIGSKWNAYLPMLETELAISKTKGQAEAVAYYMGPMSQSF
                     TGLKDAIEDAIAFNGERADVVGLEGDKAYESARFWIFSALGIALLLCFGAGFALIRSV
                     SKPLVQMTTAMGQLAAGKLDVEVPNSDQKDEIGKLAEAMTAFKNQLAAAERSKAEQTQ
                     TIVGSVGTGLEALAKGDLTHRVTAELTGPFVKLKEDFNDAMAHLQDTMKNVLTSTGGI
                     STGAGEISQAADDLSRRTEQQAASLEETAAALEEITATVKKTASNTKEASASVTAAKS
                     AAEEGGRVVETAITAMDKIEQTSKQITDIIGVIDEIAFQTNLLALNAGVEAARAGDAG
                     KGFAVVASEVHALAQRSSEAAKEIKTLIKASSEHVGAGVKFVGETGHALKRIVDQVVQ
                     INSLVTEMAQAAEQQSTGIEQVNVAVGQMDQVTQQNAAMVEESTAATRNLASETQVLS
                     NLVAFFTVGEDKKPASHNAPSVQPQTAPRPVAKAQPRPLPVRHSSGGAGRRAATAVAR
                     RPSAETEAEDWTEF"
     gene            complement(2055..>2484)
                     /locus_tag="VGC27_10430"
     CDS             complement(2055..>2484)
                     /locus_tag="VGC27_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283025.1"
                     /translation="YAAKLFGVSASTSIKWMQRWRKHKSVSPNPVRGHRRSLLEAHAD
                     WLLALIKAKPDLTLEEVRTRLKRRGARVSLWTIWNFFDKRHISFKKNSCMSNLNPKKR
                     LLKWRRNGANRFGRYNLLLRRHFHACVRTPRKNASAISAP"
BASE COUNT          392 a          748 c          779 g          565 t
ORIGIN      
        1 cgacccgttg ccggtgtcgt tcatgggaac gtctcctggc tgtcgttggc gctagaactc
       61 ggtccagtct tcggcttcgg tttcggcact gggcctgcgc gcaaccgccg tcgccgcgcg
      121 ccgtccggcc ccgccgctgg aatggcgcac gggaagcggg cgcggctgcg ctttcgcgac
      181 aggccggggt gccgtttgag gctggacgga aggcgcgttg tgcgacgcag gctttttgtc
      241 ctcgccgacg gtgaagaacg ccacgaggtt cgacaagact tgcgtctcgg aggccaggtt
      301 ccgcgttgcg gccgtgcttt cttcgaccat ggcggcgttc tgctgcgtga cctggtccat
      361 ctgcccgacg gcgacgttga cctgctcgat gccggtcgac tgctgttccg ccgcttgcgc
      421 catttcggtg accagcgaat tgatctgcac gacctggtcg acgatgcgct tcagggcgtg
      481 tccggtctcg ccgacgaatt tgacgccggc gccgacatgt tcgctcgaag ccttgatcaa
      541 ggtcttgatc tctttcgccg cctcgctgga acgttgcgcc agggcgtgga cctcgctcgc
      601 caccacggcg aagcctttgc cggcatcgcc ggcgcgcgcc gcttccacgc cggcattgag
      661 cgccagcagg ttggtctgga aggcgatttc gtcgatcacg ccgatgatgt cggtgatctg
      721 cttggacgtc tgttcgatct tgtccatggc cgtgatcgcg gtctcgacga cccggccgcc
      781 ttcttcggcc gccgatttcg cggccgtcac gctggcgctg gcttccttgg tgttggaggc
      841 cgtcttcttg accgtcgcgg taatctcttc gagcgcggcc gcggtttctt cgaggctcgc
      901 ggcctgctgt tccgtccggc gggagaggtc gtcggcggcc tgggagattt cgccggcgcc
      961 ggtcgagatg ccgcccgtgc tggtcagcac gttcttcatc gtgtcctgca agtgcgccat
     1021 ggcgtcgttg aaatcttcct tcagcttgac gaagggaccg gtcagttccg cggtcacgcg
     1081 gtgggtgagg tcgcccttcg ccagcgcttc caggccggtg ccgacgctgc cgacgatggt
     1141 ttgcgtctgt tcggccttgg agcgttcggc tgcggccagt tggttcttga aggcggtcat
     1201 cgcctcggcg agcttgccga tttcgtcctt ctggtcggag ttgggaacct cgacgtcgag
     1261 tttgcccgcc gccaattgcc ccatcgccgt ggtcatctgg accagcggtt tggatacgga
     1321 gcgtatcaac gcgaacccgg cgccgaagca aagcagcagg gcgatgccga gcgcggagaa
     1381 tatccaaaag cgggcggact cgtatgcctt gtcgccttca agccccacca cgtctgcgcg
     1441 ttcgccgttg aaagcgatgg cgtcttcgat cgcgtccttg agcccggtga acgactgcga
     1501 catcggtccc atgtaatagg ctacggcctc ggcctgtcct tttgtcttgc tgatggcgag
     1561 ttccgtctcc agcatgggca ggtaggcgtt ccatttgctg ccgatctgct ctgcgaaagc
     1621 gcgttctttg tccgaattgg ccatcgcttg gtacttgcgc agcgcttcgc cgacggcgac
     1681 ttccagcagg tccaggttct ttttgcccat cgcctgttgc tcgggcgtgg agagctccag
     1741 aaccgctccg ttccaggagc ggaagcgcgt cgcggcatac tgcacttcgc ccaaaacgcg
     1801 cgtgctctta agccaatggt ctcggatttc caccgcattg gcatccactt gggaaagccg
     1861 ctgaacggaa aatatgccga gagcgatggt tgcaatgagt acgcaagtaa acgcgctcac
     1921 cactttggcg ctgaccgaaa gatctctgaa tttcgctgcg atggacatgg gacccccaaa
     1981 tttcgcttaa gacgctgcac aaaaccgcaa tgcgctccac gcacgggttg gcgcgtacga
     2041 tgaagctggg ggatttatgg agcgctaatt gccgaagcgt tttttcgcgg cgtccgaacg
     2101 caagcgtgaa aatgccggcg caaaagcaag ttgtagcggc cgaatcgatt cgctccgttg
     2161 cgccgccact ttaatagacg tttttttggg ttgagattac tcatacacga gttttttttg
     2221 aagctgatat ggcgtttgtc gaaaaaattc caaatggtcc acaagctgac gcgggcgccg
     2281 cgccgtttca accgagtgcg aacctcttca agcgtcagat cgggtttggc cttgatgagc
     2341 gcaagcagcc aatcggcatg agcttccagc aaggagcggc gatgacctcg caccgggttt
     2401 ggtgagacgg atttgtgttt tcgccagcgc tgcatccact tgatcgacgt gcttgcgctc
     2461 acgccgaaca gctttgcggc atag
//
LOCUS       DASWPU010000494         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_28396,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000494 DASWPU010000000
VERSION     DASWPU010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28396"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VGC27_10435"
     CDS             <1..1122
                     /locus_tag="VGC27_10435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0283026.1"
                     /translation="MVAYAIGFVLFGRMIDKLGAKRGYIIAVAVWTTAHLGCAFIGFL
                     PTEWVLVSFMISQAVLGLGQSGNFPAALKVVAEWFPQRERAFAIGIFNAGSNVGAILA
                     PLIVPFVLLAFGWQWAFVATGLLSILWLLVWIPFYSKPADSKRLKKAELEYIESDKFV
                     PVPQLPWLKVARVKETWAYSIGKLLTDPVWFFYSFWLPPYFIETYRTQAGDIAGVAIP
                     VIVIYLISDVGSVAGGWMSSKLIKTGMSVNRARKLTMLLCAACVLPVAFMTTLTHDLW
                     ITTFVIGIAAAAHQAFSANLMTLPSDLMPKEAVGSVSGIGGTAGAIGGIIMAQGVATA
                     LRGMGGYSSVFLVAGLIYFLAILVVHVISPRLERAKLAG"
     gene            complement(1346..>1822)
                     /locus_tag="VGC27_10440"
     CDS             complement(1346..>1822)
                     /locus_tag="VGC27_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0283027.1"
                     /translation="LTVLGGAKLVLDTSGPPLKHALDLGVFLVKPSLSEFSGLLDQSN
                     PDQAACLAAARNLVVNGKAEIVALTMGGDGAMLVSREVALYAKPPPVTIRGTVGAGDS
                     FLGLLILSIARKMSLPDAFRQAVAAGSAALLSPGTGLCEPSDVAELAGQVQITQLL"
BASE COUNT          298 a          583 c          585 g          356 t
ORIGIN      
        1 atggtcgcct atgccatcgg cttcgtgctg ttcggccgca tgatcgacaa gctcggcgcc
       61 aagcgcggtt acatcatcgc cgtcgcggtt tggaccacgg cgcatctcgg ctgcgccttc
      121 atcggctttt tgcccacgga atgggtgctg gtgagcttca tgatcagcca ggccgtgctg
      181 ggcctcggcc aatcgggcaa cttcccagcg gcgctgaagg tggtggcgga atggttcccg
      241 cagcgcgagc gcgccttcgc catcggcata ttcaacgcgg gctccaacgt cggcgccatc
      301 ctggcgccgt tgatcgtccc gttcgtgctg ctcgccttcg ggtggcaatg ggccttcgtc
      361 gccacgggcc tgttgagcat cttgtggctg ctggtgtgga ttccgttcta cagcaagccg
      421 gccgacagca agcggctgaa gaaggccgaa ctcgagtaca tcgagagcga caagttcgtc
      481 cccgtgccgc agcttccctg gctcaaggtg gcccgcgtca aggagacttg ggcctattcg
      541 atcggcaagc tcctcaccga tccggtgtgg ttcttctatt cgttctggct gccgccctat
      601 ttcatcgaaa cctacaggac ccaagccggc gacatcgcgg gcgtggccat accggtgatc
      661 gtgatctacc ttatctccga cgtcggctcg gtggccggcg gctggatgtc ttccaagctg
      721 atcaagaccg gcatgagcgt gaaccgggcg cgcaagctca ccatgctgtt gtgcgccgcc
      781 tgcgtgctgc ccgtcgcctt catgaccacg ctgacccacg atctgtggat caccaccttc
      841 gtcatcggca ttgcggccgc ggcccatcag gcgttctccg ccaatctgat gaccttgcca
      901 tcggacctga tgccgaaaga agccgtgggc tccgtttcgg gaatcggagg cacggcgggt
      961 gcgatcggcg gcatcatcat ggcccagggc gtcgccaccg cgctgcgcgg catgggaggc
     1021 tattcgtcgg tgttcctggt ggcggggctg atctatttcc tcgcgatcct cgtcgttcac
     1081 gtgatctcgc cgcgccttga gcgcgccaag ttggccggat aagagacggc cttagagcgt
     1141 gattcatcca aatgaaattg cgccgtgttc atgcttcgac aagctcagca tgaaggattg
     1201 aacactctcc cctcaccctg agcatgtcga agggtgaggg ggagcgattc agccagacgt
     1261 gatcatgttc tagaggcccg aaaacgcttc gacccgcctg cgccgatcat ccggcgggcg
     1321 ggtcgaaagt tttaggcgtc tcgtattaca gcaactgggt gatctggacc tggcccgcca
     1381 attccgcgac gtcgctcggt tcgcacagtc cggtgcccgg cgaaagcagc gccgcgctgc
     1441 ccgcggccac cgcctgacgg aaggcgtcgg gcagggacat cttgcgggca atcgacagga
     1501 tcagcaggcc caggaagctg tcgccggcgc ccaccgtgcc gcggatcgtc accggcggcg
     1561 gcttggcgta gagggcgacc tcgcgcgaga ccagcatggc gccgtccccg cccatggtca
     1621 gggcgacgat ttcggctttg ccgttgacca ccaggttgcg cgccgccgcg aggcaggccg
     1681 cctgatcggg attgctctgg tcgagcaggc cggagaattc gctcaagctc ggcttcacca
     1741 gaaagacgcc cagatcgagg gcgtgtttga gcggcgggcc ggaggtgtcg agcaccagct
     1801 tggcgccgcc cagcaccgtc ag
//
LOCUS       DASWPU010000495         3999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_9803, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000495 DASWPU010000000
VERSION     DASWPU010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9803"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..891
                     /gene="rlmB"
                     /locus_tag="VGC27_10445"
     CDS             118..891
                     /gene="rlmB"
                     /locus_tag="VGC27_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY0283028.1"
                     /translation="MPRKPQPKDRRHFYPRPDAKASDGLWLYGLHAVRAALTNPRRTV
                     KRAVLTERAKEEIGAKLLGRIRHEVADGDAVARLAPPGAVHQGVALSCEPLPKLGLED
                     ALAPSARRRIVLVLDQISDPHNAGAILRSAAAFGVSAVIVQDRHAPPESGALAKSASG
                     ALDIVPVVTVVNIARALEQLGELDFWRIALAGDGEASLKDAAGTGDVALVLGSEGSGI
                     RRLVRERCDTAAFLPIETAMESLNVSNAAAVALYELRRN"
     gene            complement(967..1500)
                     /locus_tag="VGC27_10450"
     CDS             complement(967..1500)
                     /locus_tag="VGC27_10450"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0283029.1"
                     /translation="MYVFQKQASGDLNLLYNWPASTGREVLWPGPSGRMLKTDTPQGY
                     YQLDSERMYRRYHSSQWNQPMPYAMFFNWEHDGIQTGLAIHSAHGDDIALLGKRASAG
                     CIRLAPQNAQFLYRMIRKNYRGLAPRFAYDRRTATMHNDGHLMRDADGRLKYAEGYKV
                     LVVIENNGGDNIVAALF"
     gene            complement(1558..2091)
                     /locus_tag="VGC27_10455"
     CDS             complement(1558..2091)
                     /locus_tag="VGC27_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283030.1"
                     /translation="MSTLKALSLVYLASASAFAVVIALHSDARWAEGVRGAARVATPV
                     VRSTAETLNEKAVKPSVAWASQKGKEFFDWLELRGPQVAQQTPPKKPAPIVQAQRTTA
                     PARAATPARAPASPVLRPAITEPQSKRVTAPPTLELAPQAAEPELTPAPDANPPSAAE
                     LARVLSHMKLSLTKELF"
     gene            2652..3119
                     /locus_tag="VGC27_10460"
     CDS             2652..3119
                     /locus_tag="VGC27_10460"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0283031.1"
                     /translation="MMQEFALAATLLMAVTAANAAAVSTDKDVAAKIVAMERAALDRS
                     DKGDVQRFLELSAPDVVYQDPALTKPIVGIEALTKYYKSFPSEASQTGEMVNSNVQVF
                     GTIAVLSFHYIVRRDNTVIREWNCTEVYRQDGADWRIVNTHWSLTLPPGQKLG"
     gene            3124..3570
                     /locus_tag="VGC27_10465"
     CDS             3124..3570
                     /locus_tag="VGC27_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0283032.1"
                     /translation="MPEKNQVLALMDAAEAVYLATIGEKGPRIRALVNLRRRDLYPGP
                     SKTCRTDDFTAFLSTSMASTKVRDIRANPAVALYYCAPNQFHGVMLSGKMEILTDTEL
                     KYALWSDDWRIYWPTGTDDPDYAVLRLKADEVTGWWGSTPFRIALT"
     gene            3567..3995
                     /locus_tag="VGC27_10470"
     CDS             3567..3995
                     /locus_tag="VGC27_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0283033.1"
                     /translation="MSATRQGGFLIAKVHQAGGRIFARILRERGMEINPAQGRILFVL
                     WQEGPISIYELAKQVSLGKSTLTSALDRLEASGEVKRVRSSEDRRKIVIELTPQNKET
                     QKLYEEVSRKMTKLFYRGFADSEIARFERDLERILGNLSE"
BASE COUNT          757 a         1273 c         1233 g          736 t
ORIGIN      
        1 gccgcaccat ccacttctgg ccaaaaatca tacgttacac gtgtaacatg agtaacacac
       61 tgctacaagc ttggccgcaa gttgtctgtc cgctcgctcc cctgtaagtc cttagctatg
      121 ccaagaaagc cccagcccaa ggatcgccgc catttctatc cccgcccgga cgccaaagcc
      181 tcggacggat tgtggcttta cggtcttcat gccgtccgtg cggcactgac caacccccgc
      241 cgtacggtca agcgggccgt gctgaccgaa cgggccaagg aagagatagg cgccaagctg
      301 ctggggcgaa tacggcacga agtcgcggac ggggatgccg tggcccgcct tgcgccgccc
      361 ggcgccgtcc accaaggcgt ggcgctgtcc tgcgagccgc tgcccaagct tggcctggag
      421 gacgcgctag ctccgtctgc ccgccggcgc atcgtgctcg tgctcgatca gatctccgat
      481 ccgcacaatg cgggcgcgat cctgcgctcg gcggcggcgt tcggtgtttc ggccgtgatc
      541 gtacaggaca gacatgcgcc gccggaatcg ggcgcgctgg ccaaatcggc ttcaggcgcg
      601 ctggacatcg tgccggtcgt aacggtggtc aacatcgccc gggcgctcga acagctcggc
      661 gagctcgatt tctggcgcat cgcgctggcg ggcgacggcg aagcctcgct caaggacgcc
      721 gcgggcacgg gcgacgtcgc cctggtgctc ggctcggaag gctcgggcat ccgccggctg
      781 gtgcgcgagc gctgcgacac ggccgccttc ctgccgatcg aaaccgcaat ggaaagcctc
      841 aacgtctcca acgccgccgc ggtcgcactt tacgaactca ggcggaattg acacgctcat
      901 gcttcgacaa gctcagcatg agggaaaagt tttccttcac cctgagcttg tcgaagggta
      961 aatgactcag aacagcgccg ccacgatgtt gtcgccgccg ttgttctcga tgacgacgag
     1021 caccttgtag ccttcggcgt atttcagacg gccgtcggcg tcgcgcatca agtggccgtc
     1081 attgtgcatg gtggcggtgc gccggtcata ggcgaagcgc ggcgccagtc cccgatagtt
     1141 cttgcggatc atgcgataca gaaattgcgc gttctgcggc gccagccgga tgcagcccgc
     1201 gctcgcccgc ttgcccagca acgcgatatc gtcaccgtgc gcgctgtgga tcgccaaccc
     1261 ggtctgtatt ccgtcatgtt cccagttgaa gaacatggcg tagggcatcg gctggttcca
     1321 ttgcgacgag tgataacgcc ggtacatccg ctcggaatcg agctggtaat agccttgcgg
     1381 cgtgtcggtc ttcagcattc gccccgacgg gcccggccat agcacttcgc gccccgtcga
     1441 tgccggccaa ttgtatagca ggttcagatc gccgctcgcc tgcttctgga acacatacat
     1501 gcgctgcgac cacgggccgt ggtccgcgtt gctgatgtag agaaagagtt cgaagttcta
     1561 gaacagctcc ttggtcaggc tgagcttcat gtgcgagagc acgcgcgcca gctcggcggc
     1621 gctgggcgga ttggcgtcgg gcgcgggcgt cagctccggt tcggccgcct gcggcgcgag
     1681 ttcgagcgtg ggcggagcgg taacccgttt gctctgcggc tcggtgatgg ccgggcgcaa
     1741 gaccggcgat gcaggcgcgc gcgccggcgt ggccgcacgc gccggcgcgg ttgtacgctg
     1801 cgcctggacg atgggcgcgg gctttttcgg cggcgtttgc tgcgcgacct gtggcccccg
     1861 cagttcgagc cagtcgaaga attcctttcc cttctgcgag gcccaggcga cggacggctt
     1921 gaccgccttc tcgttcaacg tctccgccgt gctcctgacg acaggcgtgg cgaccctggc
     1981 ggcaccgcgc acgccctcgg cccagcgcgc atccgaatga agcgcgatga ccacggcaaa
     2041 ggccgacgcg ctcgccagat agacgaggct gagggccttt aacgtcgaca tcgcgggttt
     2101 cgctcgcatc gccctacgat aaccaaaagc aatacggttt gtctcgttcc gggccatcat
     2161 gcagttaatg gataacggga aaaaggcgga atccagcggc ggatttgttt acgccgcgcc
     2221 aacgccggat cgaatcatct cgccccttgg aggctacggc tatcgctcgt agcgcgcctc
     2281 tggcaacggc cggcgcaaag gacaccagaa cctcaaaacg ccaggatttg cctggggtgt
     2341 tgtttcaaga aaaaacggga ggggtacccc ttctgtcatt ccaccctgcg ttccaattca
     2401 gtctcaggcc acgccctccg caatataatt gttcctccga ggtgccagcc cctcgtcctc
     2461 ttcctgtttc ccggcgtcgg cgccagagcc ggttggcgcc attataaggc cggttgcgag
     2521 gcattggata agatggacgg gcatgcgggt ccggttttcc gggcgcgaaa aatcgcatcg
     2581 gccatccgct tgacatttag ttcgatatcg tactatctgt tctcgaacta tcgaacacag
     2641 agggaaaaac aatgatgcaa gagtttgcac ttgccgcaac gctgctgatg gccgtcacgg
     2701 ccgcgaatgc ggcggcggtt tccactgaca aggacgtagc cgccaagatc gttgcgatgg
     2761 agcgcgcggc gctggaccgc tcggacaaag gcgacgtcca gcgcttcctc gaactctcgg
     2821 cgccggatgt ggtctatcag gatcccgccc tgacgaagcc cattgtcggc atcgaagccc
     2881 tcaccaaata ctacaagagc ttcccgagcg aagcctcaca gacgggagag atggtcaact
     2941 ccaacgtcca ggtgttcggc acgattgccg tcctgtcgtt tcactacatc gtccggcgcg
     3001 acaacaccgt gatccgggaa tggaactgca ccgaagtcta tcgccaggac ggcgcggact
     3061 ggcgtatcgt caacacgcac tggtcgctga ccctgccccc cggccagaaa ctgggctgag
     3121 gccatgccgg agaaaaatca ggtcctcgcc ctgatggacg cagcagaggc agtctatctt
     3181 gcgaccatcg gggaaaaagg cccacgcatc cgcgcgctgg tcaatttgcg gcggcgcgat
     3241 ctctatcccg gcccaagtaa gacctgccgc accgacgatt tcacggcgtt cttgtctacc
     3301 tctatggcgt ccaccaaagt gcgggatatc cgggccaatc cggctgtcgc gctctattac
     3361 tgcgcgccca atcagttcca cggcgtcatg ctttcgggga aaatggagat actcacggac
     3421 acggaattga aatacgcgct gtggtccgac gactggcgca tctattggcc caccggcacg
     3481 gacgaccccg attacgccgt gctccgcctc aaggccgacg aagtgaccgg ctggtggggc
     3541 agtacgccgt tccgcatcgc gcttacatga gcgcaacgcg acaaggcgga tttctgatcg
     3601 ccaaggtgca ccaggcggga gggcgcatct tcgcgcgcat ccttcgcgaa cgggggatgg
     3661 agatcaatcc ggcccagggg cgcattctgt tcgtgctctg gcaggaagga ccgatctcga
     3721 tctacgagct ggccaagcag gtttcgctcg gcaaatccac gctgaccagc gcgctggacc
     3781 ggctggaagc ctccggcgaa gtgaagcggg tgcgctcgtc cgaggaccgc cgcaagatcg
     3841 tcatcgaact gacgccgcag aacaaagaga cccagaagct ctacgaggaa gtctcgcgca
     3901 agatgacgaa gctgttctat cgcggcttcg ccgacagcga gatcgcaagg ttcgaacggg
     3961 acctcgaacg catcctcggc aatctgtcgg agtagaaca
//
LOCUS       DASWPU010000496         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_3911, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000496 DASWPU010000000
VERSION     DASWPU010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3911"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(372..1064)
                     /locus_tag="VGC27_10475"
     CDS             complement(372..1064)
                     /locus_tag="VGC27_10475"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY0283034.1"
                     /translation="MLALLLAGCADVPDGAALTEDPYEQTNRRIFDFNQKLDQHIARP
                     VAKAYVQILPEPARDGVHNVLTNLSQPVVFANELLQGHVVAAGETVGRVAVNSVFGIG
                     GLIDVASKVDLPAHETDLGITLGKWGLEQGPYLVLPLLGPAPPRDMAGKIGDIFLNPM
                     YYVSFNGKLYYEAGLATLNVVDLRARNLDTLDSVERTSIDYYASIRSLYLQHRNGKVN
                     DRLPDAPETPDQ"
     gene            complement(1312..2163)
                     /locus_tag="VGC27_10480"
     CDS             complement(1312..2163)
                     /locus_tag="VGC27_10480"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY0283035.1"
                     /translation="MTVTDRARMKTFAPFPIAVLTAALLSSSANAGEPPASQDDWKFT
                     LSIASLYSPAFTGSKDYQAMVVPDLKIEYKDRLFISLFDGIGYNVINSHGWRAGPIAK
                     FDFGRQEDDSNPFRIAGKKTNALRGLGDIDPTAELGGFLEYSLDPVSFKVELRQGVNG
                     HKGFIGEVSVDYTDMIEVFGPPILFGIGPRLTFADSRYNNAFFGINQIQSFRSGLPQY
                     DAKTGLLSYGVGGFAMMQVTDTISVSVFGGYDRLGDQAAKSPLVKQRGSANQFSLGLA
                     VSYQFGL"
     gene            2288..>3374
                     /locus_tag="VGC27_10485"
     CDS             2288..>3374
                     /locus_tag="VGC27_10485"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0283036.1"
                     /translation="MKHWFGIISAQFKRLWFQLAVSYTVLAFCGLSLLIAMFYGLDDY
                     GDFQSAITLANVEERVASEKITVAQVIRDPANTGWRDKARNAIREKLINLERGSGSSI
                     YRITSSSRPEVFIQVRNRNGRIVMADPAIFPQGVAARFAGSPPSTAKTNVARLEADGP
                     IWVDMPVTDDNGGVIGHLRVLFIAKFDLWIEIRSIFGFLLFAWGSLFLCSVPIGIACG
                     LVASRYVTKHLKKMNDVTERWRQGDFAPRIELPNDDVLIRHSQHLNDMAQDLEMYLSL
                     KQNLAVTDERNRVARELHDTVKQKLFALGLQLATAKAKAAGSEGAEEHIREAEAITRE
                     AQRDLMEVITQLRPAKTGDVGFYERRGM"
BASE COUNT          729 a          939 c          969 g          737 t
ORIGIN      
        1 tgaccgcctt gccgccgctg gtcaggcgga tcccgaaatg gccgcgttcg aaaagcgtga
       61 gtccaagctc atcctcaagg cgggcgatgc gacggcttac ggtcgacact cgaacgccaa
      121 gagttgttgc ggcctgccca aaattgctac ttttcgcggc ttcggaaaga tatagaagag
      181 ctgcgtgatc gagcacatca cccgactttt ttgggggcgc cctcccctct ccaggattcg
      241 tattttgtgg acgcgcgctc atcgacgata attccactgg atgtacgcag gtgtagggca
      301 cgcccaatct gcacctacca acaatgatcg gtagtagtcc gccacatgcc ttatgattcg
      361 gctgcaacag ttcattgatc cggcgtctcc ggcgcatcgg ggaggcgatc attaaccttc
      421 ccgttgcgat gttgcaggta aaggcttcgt atcgaggcgt agtagtcgat cgatgtccgt
      481 tcgacgctat cgagcgtatc caggttgcgc gctctcaaat cgaccacatt caacgtggcg
      541 aggcccgcct cgtaatagag ctttccattg aatgacacgt aatacatcgg attaagaaaa
      601 atatcaccga tcttgccggc catgtcgcga ggaggcgcgg ggccaagtag cggcagaaca
      661 agataggggc cttgctccaa accccatttt cccagtgtga tccccaagtc ggtttcgtgt
      721 gccggcaagt ctactttgga tgccacatcg attaggccgc caatgccgaa taccgaattg
      781 acggccacgc gaccgacggt ttcgccggcg gcgacgacgt gcccctggag caattcgttt
      841 gcgaacacga cgggttggct gaggttggtc aggacattgt ggacaccatc gcgcgccggt
      901 tcgggcagta tttggacata ggccttggca accggacgcg ctatgtgttg gtccaacttt
      961 tgattgaagt cgaatatccg cctgttcgtc tgctcatagg gatcctccgt cagagccgcg
     1021 ccgtccggta catccgcgca gccagcaagc aggagtgcca acatgatggt gtgcgcccat
     1081 gtgcgcccct gagggaggac accaggagta gctctgctgt gttttcccgt agccgacttg
     1141 gggaaccggt tcggtagaac ggcagcacca ctgtgccact cctggtgatt tggaggatgg
     1201 tcttcttgct ttaagccacg attacagcga agccatccgt caaagtctga acggcaagca
     1261 atttgccgcc cacaattttc gtggactatc cgcaaagcaa cggacctcaa gctaaagtcc
     1321 gaactgatag ctgacggcga ggccaaggga gaactgattt gcgctgccgc gttgcttgac
     1381 gaggggcgat ttggcggctt gatcgccaag cctgtcgtat ccgccaaata cgctgacact
     1441 gatggtgtct gtgacctgca tcatcgcaaa cccgccgacg ccatacgaga gcaatccggt
     1501 tttggcgtca tattggggca accccgatct gaaggattgg atctggttga taccgaaaaa
     1561 cgcattgtta tagcgagagt ccgcaaaggt caggcgcgga ccgatgccaa acaagatcgg
     1621 tgggccgaat acctcgatca tatcggtgta gtccacgctg acttctccaa taaagccctt
     1681 gtggccattc acgccttggc gcagttccac cttaaagctg acgggatcga ggctgtattc
     1741 caagaacccg ccaagttccg cagttgggtc gatatcgccc agaccgcgca acgcgttcgt
     1801 cttttttccc gcgatacgga acggattgct gtcgtcctcc tggcgcccga aatcgaattt
     1861 cgcgatcgga ccggcgcgcc agccgtggct gttgatgacg ttgtatccaa tgccatcaaa
     1921 aagggagata aacagccggt ccttgtactc gatcttgaga tcgggcacga ccatcgcttg
     1981 gtaatccttc gagccggtaa acgccggact gtacaggctg gcgatgctca gcgtgaattt
     2041 ccagtcgtcc tgcgaggccg gtggttctcc agcgtttgcg gacgatgaaa gcaacgccgc
     2101 ggttaaaacg gcaatcggaa atggtgcgaa ggttttcatg cgggccctgt cggtaacggt
     2161 catcgatcgg gctgcatgct agcgaccctt cgcccaggcg catcggcctt aagtctgagg
     2221 tcagggcagc tgattctgtc cgacttaagg aggatgaaat gtcggctttt ctctctataa
     2281 tgcgtggatg aagcactggt ttggtatcat ctcggctcaa ttcaagcgtc tgtggtttca
     2341 actcgcggta agctacaccg tgcttgcctt ctgcgggctg agcctgctga tcgccatgtt
     2401 ctacggcctg gacgactacg gcgatttcca aagcgcaatt acgcttgcca atgtcgagga
     2461 gcgggtcgcc agcgaaaaga ttaccgtcgc gcaggtcatc cgtgatcctg ccaatacagg
     2521 atggcgcgac aaagcacgga atgccatccg cgaaaagctg atcaacttgg aacgcggcag
     2581 cggttcttcg atttaccgca ttaccagttc cagccgcccc gaggtcttta ttcaagtccg
     2641 gaacagaaac ggccggattg taatggcgga tcccgccata tttcctcagg gcgtggcagc
     2701 ccgttttgcc ggatccccgc catcgaccgc aaagacgaat gtggcacgcc tggaagcgga
     2761 tgggccgatc tgggtggata tgccggtcac ggatgacaac ggcggcgtca taggccatct
     2821 gagggttctg ttcattgcca aattcgatct ctggatcgag attcgaagca tcttcggttt
     2881 tctacttttt gcgtggggct ctttattctt gtgctcggtg ccgatcggca ttgcctgtgg
     2941 tctggtcgcc tctcgttatg tgacaaagca cctgaaaaag atgaacgacg tcaccgaacg
     3001 atggcgacaa ggcgatttcg caccacggat cgaactgccg aacgacgacg tgcttatccg
     3061 ccacagccag cacctcaatg atatggcgca ggaccttgag atgtacctga gcctgaagca
     3121 gaatcttgcc gtaaccgacg aacgcaaccg cgtcgcgcgg gaactgcacg acaccgtaaa
     3181 gcagaagctc ttcgctctcg gtttgcaact ggcaacagcc aaggccaagg cggccgggtc
     3241 ggaaggtgcc gaggagcaca tccgcgaggc ggaggccatc actcgcgagg cgcagcgtga
     3301 tctgatggag gtcatcaccc aactgcgacc tgccaagact ggcgatgtgg gattttacga
     3361 gcggcgtggc atga
//
LOCUS       DASWPU010000497         4005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_23019,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000497 DASWPU010000000
VERSION     DASWPU010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23019"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGC27_10490"
     CDS             <1..243
                     /locus_tag="VGC27_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY0283037.1"
                     /translation="GGIIIPDTVQEKPQEGEIIAVGPGALDENGKRVVPEVKPGDIVL
                     FGKWSGTEVKLDGEELLIMKESDIMGIIEGKKAAKK"
     gene            316..1959
                     /gene="groL"
                     /locus_tag="VGC27_10495"
     CDS             316..1959
                     /gene="groL"
                     /locus_tag="VGC27_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0283038.1"
                     /translation="MAAKDVRFGADARERMLRGVDILADAVKITLGPKGRNVVIEKSF
                     GAPRTTKDGVTVAKEIELHDKFENMGAQMVREVASKTNDAAGDGTTTATVLAQAIVRE
                     GTKSVAAGMNPMDLKRGIEKAVEAVVADLKKRSKKVKSNDEIRQVGTISANGDTTVGQ
                     MIAEAMAKVGNEGVITIEEAKSLDTELEVVEGMQFDRGYISPYFITNADKMVAELEEP
                     YILIHEKKLTSLQPMLPILEAVVQAGRPLLIIAEDIEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAIVTGGQVISEDLGIKLENVKINTLGTAKKVRITKDDTTLID
                     GAGKKADIQARVAQIKTQIEETTSDYDKEKLQERLAKLAGGVAVIRVGGATEVEVKEK
                     KDRVDDALNATKAAVEEGIIPGGGAALLYATKSLTGLSGDNDDQNVGIAIVRKAIQSP
                     IRQIVENAGVEASIVVGKLLDQKSVNYGYNAQTEEYVDLIEAGIVDPTKVVRIALQNA
                     SSVAALLITTEAMVADRPKKESAPAMPGGGMGGMGDMDY"
     gene            complement(2104..2442)
                     /locus_tag="VGC27_10500"
     CDS             complement(2104..2442)
                     /locus_tag="VGC27_10500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY0283039.1"
                     /translation="MNYASFDIVVVPFPYSDRLAEKRRPALVVSAPALQKRFGLIWLM
                     MITSAGNPAWDCDVAISDLKLAGLPASSVVRPAKIATVDAARILRRIGKLTARDAKRV
                     KEKLRELAAL"
     gene            complement(2444..2665)
                     /locus_tag="VGC27_10505"
     CDS             complement(2444..2665)
                     /locus_tag="VGC27_10505"
                     /inference="COORDINATES: protein motif:HMM:NF027267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HEY0283040.1"
                     /translation="MNAPFSKITSKSQTVIPKAVREKLGLKPGDLLRYVFDGKRVVIE
                     KARAEAEDDPFATFGEWGSAADEKAYRDL"
     gene            complement(2723..3280)
                     /locus_tag="VGC27_10510"
     CDS             complement(2723..3280)
                     /locus_tag="VGC27_10510"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0283041.1"
                     /translation="MIVIEFLVFVISSGLFCSNRFRGNIYAVLIAGGLATATSLLFVY
                     HVWAMLAFQPEAPVKVVKQVVHVPVVQKISQPPALSKPQDCHEDYPFWSRLFGDEGTT
                     ELAFQVLADGTVDGVSVANSSGSGRLDNAAVNCVKKWRYRPGIKDGQLTDMPMNVSVA
                     WSLADPAETTEQTAEQTTEQTTEKE"
     gene            3504..3770
                     /locus_tag="VGC27_10515"
     CDS             3504..3770
                     /locus_tag="VGC27_10515"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEY0283042.1"
                     /translation="MPTISFFYGIAIRMYFRDHAPPHFHAVYGEHEAFVSIETGEILV
                     GYLPKTATRLVGEWALARRTELRENWRRAQQSQPLERVAGLQDE"
     gene            3763..>4005
                     /locus_tag="VGC27_10520"
     CDS             3763..>4005
                     /locus_tag="VGC27_10520"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEY0283043.1"
                     /translation="MSELSLIKVTKVEPLGGHRLHLRFSDSSAGERDFANIVAEGGPM
                     LEPLRDPGYFARVFVEFGAPTWPNGYDLAPHALHAEM"
BASE COUNT          860 a         1257 c         1249 g          639 t
ORIGIN      
        1 ggcggcatca ttattcccga caccgtccag gagaagccgc aggaaggcga gatcatcgcc
       61 gtcggccccg gcgccctcga cgaaaacggc aagcgcgtcg ttcccgaagt gaagcccggc
      121 gacatcgtgc tgttcggcaa atggtcgggc accgaagtga agctcgatgg cgaggaactc
      181 ctgatcatga aggagtccga catcatgggc atcatcgaag gcaagaaggc cgcgaagaaa
      241 taaaacgagt agcgaagtag cgagtagcga atagaggcta ttcgactgca aaacggaatt
      301 caggagaaca acaacatggc tgccaaagac gtaagattcg gcgccgatgc gcgtgagcgg
      361 atgctccgcg gcgtcgatat tctcgccgat gcggtgaaga tcacgctcgg ccccaagggc
      421 cgcaacgtgg tgatcgagaa gagtttcggc gccccgcgca ccaccaagga cggcgtcacc
      481 gtcgccaagg aaatcgagct gcacgacaag ttcgagaaca tgggcgcgca gatggtgcgc
      541 gaagtcgcct ccaagaccaa cgacgcggcc ggcgacggca ctacgacggc cacggtcctg
      601 gcccaggcga tcgtgcgcga aggcaccaag tcggttgccg ccggcatgaa cccgatggac
      661 ctcaagcgcg gcatcgagaa ggcggtggaa gccgtcgtcg ccgatctgaa gaagcgctcc
      721 aagaaggtga agtccaacga cgagatccgc caggtcggca cgatctccgc caacggcgac
      781 accaccgtcg gccagatgat cgccgaagcg atggcgaaag tcggcaacga aggcgtcatc
      841 accatcgagg aagccaagag cctcgacacc gagcttgaag tcgtcgaagg catgcagttc
      901 gaccgcggct acatctcccc ctacttcatc accaacgccg acaagatggt ggcggagctg
      961 gaagagcctt acatcctgat ccacgagaag aagctgacca gcttgcagcc gatgctgccg
     1021 atcctggaag cggtggtcca ggcaggacgc ccgctgctta tcatcgccga ggacatcgaa
     1081 ggcgaggcgc tggcgaccct ggtcgtcaac aagctgcgcg gcgggctcaa ggttgccgcc
     1141 gtcaaggctc cgggcttcgg cgaccgccgc aaggccatgc tggaagacat cgccatcgtc
     1201 accggcggcc aggtcattag cgaagacctc ggcatcaagc tggagaacgt caagatcaac
     1261 accctcggca ccgccaagaa ggtgcgcatc accaaggacg acaccaccct gatcgacggc
     1321 gccggcaaga aggccgacat ccaggcgcgc gtcgcccaga tcaagacgca gatcgaggaa
     1381 accacctcgg actacgacaa ggaaaagctg caagagcggc tggccaagct ggcgggcggc
     1441 gtggccgtca tccgcgtcgg cggcgccacc gaagtggaag tgaaggagaa gaaggatcgc
     1501 gtcgacgacg cgctcaacgc caccaaggcg gccgtggaag aaggcatcat ccccggcggc
     1561 ggcgcggcgc tgctctacgc caccaagtcg ctcaccgggc tgagcggcga caatgacgac
     1621 cagaacgtcg gcatcgccat cgtgcgcaag gcgatccagt cgccgatccg ccagatcgtc
     1681 gagaacgccg gcgtggaagc ctccatcgtg gtcggcaagc tgctcgacca gaagtcggtg
     1741 aactacggct acaacgcgca gaccgaggaa tatgtcgatc tgatcgaagc cggcatcgtc
     1801 gatccgacca aggtcgtgcg catcgcgctg cagaacgcct cgtcggtcgc ggcgctgctg
     1861 atcaccaccg aagcgatggt cgccgaccgt ccgaagaagg aaagcgcccc ggcgatgccg
     1921 ggcggcggca tgggcggcat gggcgatatg gactactaaa ccccgtcatg gccgggcagc
     1981 gaccccgcac tcgatgcggg ggaagcgcga cccggccacc cagtcttcga aatacgaaaa
     2041 ggggccggag caatccggcc cctttctttt ctgccccgct tcgcggcggc ggaaaagact
     2101 gcgctacaac gccgccaatt ctcgcaactt ctcctttacc cgcttcgcat ccctcgccgt
     2161 cagtttccca atccggcgca ggatgcgcgc ggcatcgacg gtggcgatct tggccgggcg
     2221 gacgacggaa gacgcgggaa gacccgccag cttcaaatcg gaaatcgcga cgtcgcaatc
     2281 ccaggcgggg ttgccggcgc tggtgatcat catcagccag atcagcccga agcgtttttg
     2341 cagcgcgggc gcgctcacca ccaaagccgg gcggcgcttt tccgccaggc ggtccgagta
     2401 aggaaacggc accacgacga tgtcgaagga tgcgtagttc atatcagagg tcgcgatagg
     2461 ccttttcgtc ggccgcgctg ccccattcgc cgaaggtggc gaagggatcg tcttccgctt
     2521 ccgcccgcgc cttttcgatc accacgcgct tgccgtcgaa cacatagcgc aagagatcgc
     2581 cgggcttcag ccccaacttt tcgcgcaccg ccttgggaat gacggtctgc gacttcgagg
     2641 tgatcttgga aaagggtgcg ttcatggccg gcctcgcggg agcacggtaa gaatgtaaga
     2701 catcttaccg cttcgcacaa gcctattcct tttccgtggt ctgctccgtg gtttgctccg
     2761 cggtctgctc tgtggtctcc gctgggtcgg ccaggctcca cgccacgctg acgttcatgg
     2821 gcatgtccgt aagctggccg tccttgatgc cggggcggta acgccacttc ttcacgcagt
     2881 tcaccgcggc attgtcgagc cggcccgaac cgctggagtt cgccacgctc acgccgtcca
     2941 cggtgccgtc ggccaggact tgaaaggcaa gctcggtggt tccttcgtcg ccgaacagac
     3001 gcgaccagaa gggataatcc tcgtggcagt cttgcggctt cgacagcgcc ggcggctgcg
     3061 aaatcttctg caccacgggc acgtgcacca cctgcttgac gaccttgacc ggcgcttcgg
     3121 gttgaaacgc cagcatcgcc cagacatggt agacgaacag cagcgaggtg gcggtggcga
     3181 gcccgcccgc gatcagcacc gcatagatgt tgccgcgaaa acgattgctg cagaaaagtc
     3241 ccgaagaaat gacgaaaacc aggaactcga tgacgatcat acctaacccg atgctgcagc
     3301 cgttcgctga accatactag cgcggtcgat gaggcaaaaa cacggcgttc cgagagagtg
     3361 cggcaacgat gcttcggttc attaacgtgt gaaaaggccc ggacggtccg caaggtccgg
     3421 gcggcagcga agaacgcgtg cggaagccgg ggcttttgtt tatcttcgat cgtcgctatc
     3481 gtaacgccaa ggcgggaata gtcgtgccga ccatctcttt cttctatggc atcgcgatcc
     3541 gcatgtactt tcgcgaccac gcgccgccgc attttcatgc cgtctatggc gagcacgaag
     3601 ctttcgtctc gatcgaaacc ggagaaatcc tggtcggata tctgccgaag accgcaaccc
     3661 gtctcgtcgg cgaatgggcg cttgcgcggc ggacggaact gcgggaaaat tggcgccgcg
     3721 cacaacaaag ccagccgctt gagcgcgtgg cggggttgca ggatgagtga actatcgctg
     3781 atcaaggtga cgaaagttga gccgctcggc ggccatcgcc tgcacctgcg gttctccgac
     3841 agcagcgccg gggagcgcga tttcgcgaat atcgtcgccg aaggcggccc gatgctcgaa
     3901 cccttgcgcg atcccggcta ttttgcccgc gtcttcgtgg aattcggcgc gcccacctgg
     3961 cccaacggct acgatctcgc tccgcatgcg ctccatgccg aaatg
//
LOCUS       DASWPU010000498         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_28451,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000498 DASWPU010000000
VERSION     DASWPU010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28451"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VGC27_10525"
     CDS             complement(<1..394)
                     /locus_tag="VGC27_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283044.1"
                     /translation="MEAEGCINPEARRNEALRALAKRLKSNPPDHPVVAAGSTGSIPA
                     TAELLGVIARLPKGVVVLPGLDRELDEDSWQRLDPGHPQFALKQLLERIGVRREDVAD
                     WLPSAGLPGRERILREVLRPAPTTDAWRA"
     gene            complement(431..937)
                     /locus_tag="VGC27_10530"
     CDS             complement(431..937)
                     /locus_tag="VGC27_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283045.1"
                     /translation="MPRPPDIANVFTIPAGAPFSATLAQGLLRRFGTDGPFALADMTI
                     YLPTRRAARGIAEIFARESGGAALLPDFRPLGDVDEDELLLDPIADTLELKPAIAPLR
                     RRLLLATLIRRWSLARDYGRISFGQAAGLAKSLAAVMDEVERQGADLTKLADLAPLAL
                     AEHWAEVK"
     gene            complement(921..>1681)
                     /locus_tag="VGC27_10535"
     CDS             complement(921..>1681)
                     /locus_tag="VGC27_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0283046.1"
                     /translation="ARGARATKEHDMSSPTRAMIMAAGLGTRMRPLTNDRPKPLVEVG
                     GKALIDHAIDRLVAAGVTTVVVNLHYKAEMIQAHLAQRKDVEILYSDEGDGLLGTGGG
                     VALALEHFRGEPFYIHNSDTVWVEGYGAALERMKARWDPETMDALLLMAPMVTSMGYE
                     GIGDFMMDPSGRLSRVPPGRVSPFAFPGVQIVHPRLFDNAPAGGFSTNILWDRAIEKE
                     RLFGVRLDGIWIHVGTPQAVAEAEEFLADLAPAA"
BASE COUNT          287 a          562 c          554 g          278 t
ORIGIN      
        1 acgcgcgcca ggcgtcggtc gtcggtgccg gccgcagcac ctcgcgcaag atgcgctcgc
       61 gcccgggcaa cccagccgag ggcagccaat cggcgacgtc ttcgcgccta acgccgatcc
      121 gttccagcaa ttgcttcaag gcgaattgcg gatggccggg atcgagccgc tgccaactat
      181 cttcatccag ctcgcgatcc agtcccggca ggacgacgac gcctttgggc aaacgcgcga
      241 tgacaccaag cagttccgcg gtcgcgggaa tgctgccggt ggaaccggcc gccaccacag
      301 ggtgatcggg aggattgctt ttaagccgct tggccagagc gcgcagcgct tcgttacggc
      361 gcgcttcggg attgatgcag ccctcggcct ccaatagccc aggccattcg gtatggatca
      421 gcgagaggaa ttacttgacc tcggcccaat gctcggccag cgccaggggt gcgaggtctg
      481 cgagcttcgt cagatcggcg ccttgccgtt cgacctcatc catcacggcg gcgaggcttt
      541 tggcgagacc ggccgcctgc ccgaagctga tccgtccata gtcgcgcgcc aggctccagc
      601 ggcggatcag cgtcgccagc agcagacgcc ggcgcagcgg cgcgatggcc ggcttcagct
      661 ccagcgtgtc ggcgatgggg tcgagcagca gctcgtcttc gtccacgtcg cccagcggac
      721 ggaaatccgg caacagcgcc gcgccgccag attcccttgc gaagatttcg gcgatgccgc
      781 gcgccgcgcg ccgtgtcggc agatagatcg tcatatcggc gagggcgaac gggccgtccg
      841 ttccgaagcg ccgcaacagt ccttgcgcca aggtcgcgga aaagggcgcg cccgccggga
      901 tggtgaagac gttggcgatg tcaggcggcc ggggcaaggt ctgccaggaa ttcttcggcc
      961 tcggccacgg cctgcggggt gccgacatga atccatatgc cgtccaggcg gacgccgaac
     1021 agccgttcct tctctatggc gcggtcccac aatatgttgg tcgagaaacc gccggccggc
     1081 gcgttgtcga acaggcgcgg atgcacgatc tgcacgcccg gaaacgcgaa gggcgaaacc
     1141 cggcccggcg gcacccgtga aagccgcccc gaggggtcca tcatgaaatc gccgatgcct
     1201 tcgtagccca tggacgtgac catgggcgcc atcagcagaa gcgcgtccat ggtttccggg
     1261 tcccagcgcg ccttcatgcg ttcgagcgcc gcgccatagc cttccaccca gacggtatcg
     1321 gaattgtgga tatagaaggg ctcgccgcgg aaatgctcca gcgccaaggc gacgccgccg
     1381 ccggtgccga gcagtccgtc gccttcgtcg gaatagagaa tctccacgtc cttgcgctgc
     1441 gccagatgtg cctggatcat ctcggccttg taatggaggt tgaccaccac ggtcgtcact
     1501 ccggcggcga ccaggcggtc gatggcatgg tcgatcagcg ccttaccgcc gacttccacc
     1561 agcggcttgg ggcggtcgtt ggtcaagggc ctcatgcgcg tgcccagccc agcggccatg
     1621 atcatcgcgc gcgtgggtga actcatgtca tgctccttgg ttgcgcgcgc gcctctagcg
     1681 g
//
LOCUS       DASWPU010000499        13441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_23035,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000499 DASWPU010000000
VERSION     DASWPU010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 13441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13441
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23035"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1927
                     /locus_tag="VGC27_10540"
     CDS             <1..1927
                     /locus_tag="VGC27_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0283047.1"
                     /translation="RAGDLKNLDPNAKGDLNAINLNAGRAALSFAPSDRFSADLIFNY
                     QQDKTNGTGFKSMYMSPTDPATGQVLAGTKTDDPVWLSTSADFAGGRDLGVDQNYGGV
                     TGLLNYRLNDTLTLHSVTGYRDVGSIEVYDADGTSLPIYTVKEDNGGTFFSQELRLAY
                     DNGGAFSWFVGGSYYRERARSKVDIRFDERMLLAQAAGMLNGGPLLGLPATTPAPAGL
                     FANSAFTGAIVQGLAASLSQGNILMSAADASALAARLDPAHVEHQNNTADLDSYDLFG
                     DVTWRPAENWEFSAGLRYSTDNKITRWASSVDARSILGGIIGATGIAASGNPADVATA
                     KGLLAGLTAYGTSLAAPIPAFAINTQPTANNGDTTARNLDDDGLTWRLTGRYIVSASS
                     NIYASYARGRRPAVLSAAAPSAPGGTPVFTVAPAETADAYEIGYKASLGAVNFDAAFF
                     YYDYQNFQTRELVGSTFVTTNAGTAQSYGVELQGNWQADPMLDLFTTYSYTHARFGNG
                     AYKDNSFARSPDHMFSLGASVNVRGLDGMFHLRPSYSYRSKIFFADDNDKPQLQTGRI
                     VPDLIQDEYQNGFGLLNLRLAYVPDDANWQIEAFADNALDTVYRKGAGSAGESIGLPT
                     NVRGEPRVYGLRVSVHY"
     gene            1938..3011
                     /locus_tag="VGC27_10545"
     CDS             1938..3011
                     /locus_tag="VGC27_10545"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY0283048.1"
                     /translation="MHFVASLALALAAFAAGAMASPIVTAHRGASGYLPEHTASAYRL
                     AIEQGADYVEPDLVMTKDGILVARHENEIGRTTDVASHPEFAARKTTRVIDGESFTGW
                     FVEDYTFAELKTLRARERNPALRPASARHDGEETVLSLQEIIDIAKQGGDKRGRPVGL
                     YIELKNPVYHAGIGLPMETELVKILQANGLDSADAPVFIESFWPDALMKLRGLTKVRL
                     TFLMNSVAPPESILKAYGYDSYADVYSPAGLAKIARFADAVGPETELILPRGADGRSL
                     APTRFIEDAHKAGLAVHVWSLNAQNAALPLELRRGDPAAPGYAGALGDGAALAKQLFD
                     LGADGLFTDQPDIAIAGRDQARP"
     gene            complement(3233..4027)
                     /locus_tag="VGC27_10550"
     CDS             complement(3233..4027)
                     /locus_tag="VGC27_10550"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HEY0283049.1"
                     /translation="MTAGLFARNVTVAFGARKILDGVDAEVTPGTVTAIVGPNGAGKS
                     TLLKVLSGDIVPQSGKVTLNGKPLGAWSAEECALQRAVLPQSPELAFSFRAWDVVELG
                     RHPHRSRVSAEEDRKAIMGAIEAAEVSDYAGRDCRTLSGGEMHRTHYARALAQIWAPL
                     TDGRTRLLLLDEPTASLDLFHQHAILAKARSMARAGAGVLAVLHDLNLAAVYADTLIV
                     LAKGRVDITGSPHEVLAADRIARIWRVACDVIKGDSGKVQILVRPH"
     gene            complement(4024..5100)
                     /locus_tag="VGC27_10555"
     CDS             complement(4024..5100)
                     /locus_tag="VGC27_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY0283050.1"
                     /translation="MTVLSVTAFDAARDPLRMRRHIILGVLAAALVLASLAACAIGAL
                     KIPLSHIMAALAGRLGFGADVETFEAAALFTIRLPRVGLAIGVGAVLGMGGAALQALF
                     RNPLADPGLVGVSGGAACGAVGWIVLGTTLPAWMHSAAAMPLAAFACGLAATVCVYLI
                     GRNRSGADIATLLLAGLAMNAITTAVVGYLTYLGSDAQLRALTFWLLGGLGGSTWQQI
                     WPALILMAAALGGLCALMRSYDLLALGESAAGHLGLAVETVRRLTLIFVALGVGASVA
                     LTGMIGFVGLAAPHLVRLMGGAAHRYVLPASALMGSLLVVLADIFARIAVAPAELPVG
                     VVTSALGAPFFIWLLRRRLNGALA"
     gene            complement(5104..5967)
                     /locus_tag="VGC27_10560"
     CDS             complement(5104..5967)
                     /locus_tag="VGC27_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0283051.1"
                     /translation="MKTPAVSSAAFLLLALPALAAPAQRIVTLGGDVTEIVYALGQEN
                     RLVCDDETSLYPPAATKLPRVGYLRTLAAEGVLSCKPDLIIASEDAGPPAAVTQLENS
                     GVRFVRVGNAHTPDAVPVKIAAVADALGVPDRGRALQARFRSGMQRAQAQLAKFKDRP
                     RAIFLMAQGPGGTMAAGRGSAADAMLTLAGAVNVAGGFEGYKALTAESAVALKPDVII
                     VADHAVQMLGGMAALRARPEIVLTPAGKNGRLVAMDALLLLGFGPRTPEALTELAAAL
                     HRPAPLKQAAK"
     gene            complement(5964..6959)
                     /locus_tag="VGC27_10565"
     CDS             complement(5964..6959)
                     /locus_tag="VGC27_10565"
                     /inference="COORDINATES: protein motif:HMM:NF017022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HEY0283052.1"
                     /translation="MTTNRLRPRDAAHRLGVSEGELIASQVGEGAMRLNTKGAAILTQ
                     IPALGTVMALTRNAHCVHEKIGPYLDVTINAAHGLVLGELIDLRIFPSRWHHTFAVID
                     KVDGRPRRSIQVFDADGAAVHKIYEKPGTNIEAFDKLIAGWRADDQSPLLTVQAIPRP
                     DADRPDSEIDVEKLRREWSALQDTHDFFGMLRGLQVGRLQAMRLAGAEFARPVAADSL
                     ARVLEAARDTETSIMVFVGNPGCIQIHTGPVTNLKRMGPWFNVLDSAFNLHLREDAIA
                     GAYVVRKPTRDGDVTSLELFDRDGFCFAQLFGARKPGKPELEAWRAILSLLGENP"
     gene            7322..9361
                     /locus_tag="VGC27_10570"
     CDS             7322..9361
                     /locus_tag="VGC27_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HEY0283053.1"
                     /translation="MAALETVTISATRNPTSTLNYSGMVDVLDAEDISAAIPSTISDL
                     VKTIPNVQFVGGPRRTSESPNIRGLGGEDVLVLIDGVRQSWTSGHDGRFFLDPALLSS
                     MEVVRGPASALYGSGALGGVMAFRTADASDFLAPGQTFGARVALGYQDVDEEFLRTVT
                     GFTHIGNFDLIGSIGQRSSGDIRLGSGATLAADDDIVTGFAKAGYDFGGGLSAKLSYQ
                     GFKNEAVEPANGSGLSSGAPVNKTVVSQQFTGQINWKPEGFSLIDLHLTPYHVQTSVE
                     EADPTTGERTFRDIKTNGLSLDNRTSFSFANVSGLFTVGGDWYEDDQVGRDTLAPGGV
                     RSGVPNGSDSFWGMFAQIEANIDRPLGAPGKLTLVPAIRYDSYSTSSTGNPDTDETSV
                     SPKFAATYAPVNWLFAFGNVGKAFRAPGVNSLYLNGIHFSVPHPILPGVSVANTFQPN
                     PTLKPETSHYWEAGAGVVFADLISAGDSLTAKASYWEQSVDDYISLNVFTPATFYAPG
                     CFTPPTFLAGCNVGYTTSQNVNAQLSGAELQAAYDGERVRLEVDYGTMTGHERGTPYD
                     LNSLMPDIVTTVATLKLPEAEALLNARVTVAESFKRSYNPVSNDPASETREGYTLLDL
                     YATWAPRELLGGKLKGLRVDFGVDNVTDEDYTPYAAGVSAAGRNVKVLASYTLGW"
     gene            9386..10732
                     /gene="hutW"
                     /locus_tag="VGC27_10575"
     CDS             9386..10732
                     /gene="hutW"
                     /locus_tag="VGC27_10575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HEY0283054.1"
                     /translation="MLPVERHFARSGDPLAGAFVERSAGHWVRGRPVPVPDAGAAFAS
                     LVAAKRPGRRAAYLHVPFCSNHCLYCGFYRNRADEDALAVYTAYVERQIARDAEAAKG
                     FGPVHAVYFGGGTPNALAAEDLHRLIRAVRSRLALAPDCEITVEGRVFGFTPEKIDAC
                     LAAGANRFSIGVQTFDTDLRRRFGRKAEREEAVEFLRALRERDAAAVVCDLIYGLPGQ
                     DIESWRRDLRTCIELDLDGVDLYALTLFDKGPLALSIAKGALPAAASQRELAEFYAVG
                     LEMLEDAGWRHLTQAHWGSGTRERNLYNQFVKSGADCLAFGSGAGGMLGGHRYMLDGD
                     CEAYQRRVMAGDRPIAMMLAPQPHQRVRDLVTAGLEAGRLDLARLEAAAAPGLSKALA
                     PLVDQWAGAGLVRRRADSIVLTTAGWFWHGNLGASLNQLISLYLGGTPGTEVRHVH"
     gene            10935..11450
                     /locus_tag="VGC27_10580"
     CDS             10935..11450
                     /locus_tag="VGC27_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283055.1"
                     /translation="MNAHTPPIAYLDDMSLSHLNTAELLLTATLRLYVLAQRGAEGEC
                     PDWRNGMLTTGVRPCAVVAFDVLVKTILGQTQQALDVRCPLCPKLGADEGRFLQMTSL
                     LQQQRFGAAHAILGQWLPPLAQAAMMSPAVHVAFACASRGLRVPWRHGDTEMLAYFDV
                     PCGDPGVNFVH"
     gene            complement(11467..11982)
                     /locus_tag="VGC27_10585"
     CDS             complement(11467..11982)
                     /locus_tag="VGC27_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0283056.1"
                     /translation="MTSYPSHLIRPVDWLGERLLIRPVRPDDAPKFIAGALLCSAEDL
                     RFRFLRSIHHLSEKLAAQLTQIDYERHMAFVAENLKGDILAVSRLVGDTCKKSAEYAL
                     IVRTDLQKRGLGGLMHNLLLDYAAGRGITELWGIVDSDNRRALGFVRRLGFRLGFHAD
                     FPFTHIIRAAI"
     gene            12288..12539
                     /locus_tag="VGC27_10590"
     CDS             12288..12539
                     /locus_tag="VGC27_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEY0283057.1"
                     /translation="MAIPGRIVGVSDDDPVMRVGRVDFGGIVKEINLAYVPEARVGDH
                     VLVHVGFALTVIDEAEADRLFEQLSEIAEIEAAQMEATQ"
     gene            12536..>13441
                     /gene="hypD"
                     /locus_tag="VGC27_10595"
     CDS             12536..>13441
                     /gene="hypD"
                     /locus_tag="VGC27_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEY0283058.1"
                     /translation="MRQAEAYRDGTLARALAARIAREAGPSVDYNFMEFCGGHTHAIS
                     RYGIEDLLPANVRMIHGPGCPVCVLPVGRIDTAIRLAGRPEVTLCVYGDLMRVPGSHG
                     SSLLKAKAAGADVRMVYSTLDALRIAEAEPERQIVFFAIGFETTTPPTALAVKLAAAK
                     KLANFTIFCNHVLTPSAMQSILESPDVRLDGFVGPAHVSTVIGSRPYAFVAEEYKKPI
                     VIAGFEPLDVMQAILMLVRQVNEGRHEVENQYARAVKPEGNEAAKAEMAEIFELRKSF
                     EWRGLGQVPYSGLRLKETYAAFDGER"
BASE COUNT         2437 a         4343 c         4208 g         2453 t
ORIGIN      
        1 gcgcgccggc gatctgaaga atctcgatcc gaatgccaaa ggcgacctca acgccatcaa
       61 cctcaatgcc ggacgcgcgg cgctcagctt cgcgccgagc gatcgtttca gcgccgacct
      121 gatcttcaat tatcagcagg acaagaccaa cggcaccggc ttcaaatcca tgtatatgag
      181 tccgaccgat ccggcgacgg ggcaggttct ggccggcacc aaaacggacg atccggtgtg
      241 gctctccacg tctgcggatt ttgccggggg ccgcgacctc ggcgtcgatc agaattacgg
      301 cggcgtcacg ggcctgttga attatcgcct gaacgacacg ctgacgctgc attcggtcac
      361 cggctatcgc gatgtcggct ccatcgaggt ctatgatgcc gacggcacct cgctgccgat
      421 ctatacggtc aaggaagaca atggcggcac cttcttcagc caggagcttc gcctcgccta
      481 tgacaatggc ggcgccttct cctggtttgt cggcggcagc tattatcgcg agcgtgcccg
      541 ttccaaggtc gatatccgct tcgacgagcg gatgcttctg gcccaggccg cgggcatgct
      601 gaacggcggg ccgctgcttg ggctgccggc gaccacgccg gccccggcgg gcctgtttgc
      661 caatagcgcc ttcaccggag cgatcgtgca gggcctcgcc gccagtctca gccagggcaa
      721 tatcctgatg agcgcagccg atgcctcggc ccttgcggcc cgcctcgacc cggcgcatgt
      781 cgagcatcag aacaacaccg ccgatctcga ttcctacgat ctgttcggcg acgtcacctg
      841 gcggccggcg gagaattggg aattcagcgc cggtctgcgc tacagcaccg acaacaagat
      901 cacacgctgg gccagttccg tggatgcgcg cagcattctc ggcggcatca tcggcgccac
      961 cggcatcgcc gccagcggca acccggcgga tgtagcgacg gccaaagggt tgctggccgg
     1021 gctgacggct tacggcacct cgcttgccgc ccccattccc gctttcgcga tcaacaccca
     1081 gcccacggcc aacaacgggg atacgaccgc ccgcaacctc gatgacgacg ggctgacctg
     1141 gcgtctgacc ggacgttaca tcgtttcagc cagttccaat atctatgcca gctatgcgcg
     1201 cgggcggcgc ccggcggtgc tctccgccgc cgcgccttcg gcgccgggcg gcacgccggt
     1261 cttcaccgtg gccccggccg agaccgccga tgcctatgaa atcggctaca aggcaagtct
     1321 cggcgcggtg aatttcgacg ccgcgttttt ctattacgac tatcagaatt tccagacgcg
     1381 cgagctggtg ggttccactt tcgtcaccac caacgccggc acggcgcaat cctatggcgt
     1441 ggagttgcaa ggcaattggc aggccgatcc gatgctggat ctcttcacca cctattccta
     1501 tacccatgcc cggttcggca atggagcgta taaggacaat agtttcgccc gttcgcccga
     1561 tcacatgttc tcgttgggcg catccgtgaa cgttcgcggg ctcgacggaa tgtttcatct
     1621 tcgtccgagc tacagctatc gcagcaagat cttcttcgcc gacgacaacg acaagccgca
     1681 gttgcaaacc ggacgcatcg ttcccgatct gatccaggac gaataccaga acggcttcgg
     1741 gctgctcaat ctgcgccttg cttatgtgcc cgatgatgcg aattggcaga tcgaggcttt
     1801 cgccgataac gctctcgata cggtttatcg caagggggcg ggcagtgccg gggaatccat
     1861 cggactcccc accaacgtac ggggagagcc gcgggtctac ggccttcgcg tttccgtcca
     1921 ttattaggag cgtcgtgatg cactttgtag cgtccctggc tttagccctg gcggcttttg
     1981 ccgccggggc catggcgtcc ccgattgtca ccgcccatcg cggcgccagt gggtacctgc
     2041 ccgagcacac cgcctcggcc tatcgcctcg ccatcgaaca gggcgcggat tatgtcgagc
     2101 ccgatctggt gatgaccaag gacggcatcc tggtcgcccg tcatgagaac gagatcggcc
     2161 gcaccaccga tgtcgcctcc catcccgaat tcgcggcgcg caaaaccacg cgcgtgatcg
     2221 acggcgaaag tttcaccggc tggttcgtgg aagattacac ctttgcggaa ctgaaaacct
     2281 tgcgcgcgcg cgaacgcaat cccgcgctgc ggcccgccag cgcccgccat gacggcgagg
     2341 agacggtgct ctcgctgcag gagattatcg atatcgcgaa acaaggcggc gacaagcgcg
     2401 gtcgcccggt cgggctttac atcgagctga agaatcccgt ctatcacgcc gggatcggcc
     2461 tgccgatgga aacggaattg gtgaaaattc ttcaggcgaa cggcctcgac agcgccgatg
     2521 cgccggtttt catcgagtcc ttctggccgg atgccctgat gaagctgcgc gggctgacga
     2581 aggtgcgcct gaccttcctg atgaattccg tggccccgcc cgagagtatc ctgaaggcct
     2641 acggctatga cagctatgcc gacgtctact cgccggcggg gctcgccaag atcgcccgct
     2701 tcgccgatgc ggtcgggccg gagacggaat tgatcctgcc gcgcggcgcg gacgggcgct
     2761 cgctggcgcc gacccgtttc atagaagacg cccataaggc ggggcttgcc gttcatgtgt
     2821 ggagcctgaa tgcccagaac gccgcgctgc cgctcgaact gcggcggggc gaccccgccg
     2881 cgccgggcta tgccggcgcg ttaggagacg gcgcggcgct ggccaaacag cttttcgatc
     2941 tcggcgcgga cggcctgttt accgatcaac ccgacatcgc catcgccggc cgcgaccaag
     3001 cccgcccgtg agaccgtgta tgtctcatca accgcagcgg tggccttttt tctcgtttat
     3061 tttccggcgg gcaaagacgg ttcgcgaagg cgtgccaaac ctgatgccgg ccggaacccg
     3121 ccagcgattg ctaaaggata atgttatgaa ggtgcgtcat gccggggttg cgcgccgctg
     3181 acgctcccag gcccaatagt ggggacgcag gccgctgttt gccgcaagac cttcagtgcg
     3241 gtctcaccag gatttggacc ttcccgctat cgcctttgat cacgtcgcag gccacgcgcc
     3301 agatgcgtgc gatgcggtcg gcggcaagca cctcatgcgg cgaacctgtt atatccacgc
     3361 gtcctttggc gagcacgatc aatgtgtcgg cgtaaaccgc ggcgagattg aggtcgtgca
     3421 agacggccaa tacgcccgcg cccgcgcggg ccatggaacg cgccttggcc agaatggcat
     3481 gctggtggaa caggtcgaga cttgcggtcg gctcgtcgag caggagcagc cttgtgcggc
     3541 cgtcggtaag cggggcccat atctgagcca atgcacgcgc atagtgggtg cgatgcatct
     3601 ctcctcccga aagagtccgg cagtcgcgcc cggcatagtc cgatacttcg gccgcctcta
     3661 tcgcgcccat aatggctttg cggtcttcct ccgcgctcac ccggctgcga tggggatggc
     3721 ggcctagttc aaccacatcc caagcccgga aagagaaagc cagctccggc gattggggca
     3781 gaaccgcgcg ttgcaaggcg cattcctccg cgctccaggc gccgagcggt ttgccgttga
     3841 gcgtcacttt gcccgattgg ggaacgatgt cgccggacag cactttcaac agcgtcgatt
     3901 tgcctgcccc attgggcccg acaatggccg tcaccgttcc gggcgtcact tcggcatcga
     3961 cgccgtccag gattttgcgg gccccgaacg cgacggtgac attgcgcgcg aatagtccgg
     4021 cggtcatgca agcgctccgt tcaggcggcg gcgcagcagc cagatgaaga aaggcgcgcc
     4081 gagcgcgctg gtgaccactc ccaccggcag ttcggccggc gccacggcga tgcgcgcgaa
     4141 gatatccgcg agcaccacga gcaagctccc catcagggcc gaggccggca gcacatagcg
     4201 atgggccgct ccccccatca atcgcaccag atgcggcgcc gcgaggccga caaaaccgat
     4261 catgcccgtc agggccacgc tggcgccgac gccgagtgcc acgaaaatca gcgtcagccg
     4321 gcggacggtt tcaaccgcca agcccagatg accggccgcg ctttcgccca aagccaagag
     4381 gtcgtaacta cgcattagag cgcacaatcc gccgagcgcc gccgccatca ggatcaaggc
     4441 aggccatatc tgctgccagg tcgagccacc gagtccgccg agaagccaga aggtcagcgc
     4501 ccgcaactgc gcgtcgctgc ccagataggt gagatagccc acgacggccg tggtgatggc
     4561 gttcatcgca aggcctgcca gcagcagggt tgcgatatcg gcgccggacc tgttgcggcc
     4621 gatgagataa acgcacacgg ttgccgccag tccacaggcg aaggcggcaa gcggcatggc
     4681 cgccgcggaa tgcatccatg cgggcagagt ggtacccagc acgatccagc cgacggcgcc
     4741 gcaggctgca ccgcccgaga cgccgaccag ccccggatcg gcgagggggt tgcggaacaa
     4801 agcctgcagt gccgcgcctc ccattcccag caccgcgccg acgccaatgg ccaggccgac
     4861 gcgaggcagg cggatggtga acagagccgc ggcctcgaac gtctcgacat ctgcgccgaa
     4921 acccaggcgg ccggccagtg ccgccatgat gtgcgaaaga ggaattttca atgcgccgat
     4981 cgcacaggcg gcaaggctgg ccagcacgag agcggcggcc agcacgccca agatgatgtg
     5041 ccggcgcatc cgcaagggat cgcgcgcggc gtcgaacgcc gttaccgaaa gaaccgtcat
     5101 ggcttacttg gccgcttgtt tcagaggggc cggacgatga agggctgcgg cgagttcggt
     5161 gagcgcttcc ggcgttcgcg gcccaaatcc caacagaagc agcgcgtcca tggcgacgag
     5221 gcggccgttc ttgccggcgg gcgtcagcac gatctcgggc cgtgcccgca aagcggccat
     5281 gccgcccagc atctgcacgg catgatcggc gacgatgatc acatcgggct tgagcgcgac
     5341 ggccgattcc gctgtcagcg ctttatagcc ttcgaatccg cccgctacat tgacggcgcc
     5401 tgccagcgtc agcatggcgt ccgctgcgga gccgcgcccg gccgccatgg ttccgcccgg
     5461 cccctgcgcc atgaggaaga tcgctctcgg gcggtccttg aattttgcga gctgtgcctg
     5521 cgctctctgc attccgctgc gaaaccgggc ttgaagcgca cggccgcgat ccggcacgcc
     5581 caaggcgtcc gccacggccg caatcttgac cggaacggca tcgggcgtgt gggcgttacc
     5641 gacgcgcacg aagcggacgc ccgaattctc aagctgcgtc accgcggcag gcgggccggc
     5701 atcttccgat gcgatgatga ggtccggttt gcaggacaaa acgccttcgg ccgccagcgt
     5761 gcgcaaatag cccacgcggg gcagcttggt cgccgcaggc ggatagagcg aggtctcatc
     5821 gtcgcagacc agccggtttt cctggcccag cgcatacacg atctcggtca cgtcgccgcc
     5881 cagcgtcacg atgcgttggg cgggtgcggc caaggcgggc aacgccagaa gcaaaaaagc
     5941 ggcagaactt acggctggag tcttcatggg ttttccccca gcaaagagag gatggcgcgc
     6001 caggcttcga gttcgggctt gccgggtttg cgggccccaa acaattgcgc gaaacagaag
     6061 ccgtcccggt cgaacaattc gagcgaggtg acatcgccgt cgcgcgtcgg cttgcgtacg
     6121 acataggcgc cggcgatggc gtcttcacgc aggtgaaggt tgaaggcgga atcgagcacg
     6181 ttgaaccacg gccccatgcg cttgagattg gtcaccggcc ccgtgtggat ttggatgcag
     6241 ccgggatttc ccacgaacac catgatggag gtttccgtat cgcgcgcggc ttccagaacg
     6301 cgcgccaggc tgtcggccgc caccggccgg gcgaattccg ctccggcgag ccgcatcgct
     6361 tgaagccgtc cgacttgcaa tccgcgcagc atgccgaaga agtcgtgcgt atcctgaagc
     6421 gccgaccatt cgcggcgcag tttttcgacg tcgatctcgc tgtcgggacg atccgcatcg
     6481 ggccgcggaa tggcttggac cgtcagcaag ggcgattgat cgtcggcgcg ccagccggcg
     6541 atcagcttgt cgaaagcctc gatattcgtt ccaggctttt cgtagatttt gtgaacggcg
     6601 gcgccgtcgg catcgaacac ttgaatggag cggcgagggc ggccatccac cttgtcgatc
     6661 acggcgaagg tatggtgcca gcgcgaggga aaaatgcgca agtcgatcaa ctcacccagc
     6721 accaatccgt gtgcggcgtt gatcgtcacg tcgaggtaag gaccgatctt ctcatgcacg
     6781 cagtgggcat ttcgcgtcag cgccatgacc gtccccagcg ccgggatttg ggtgaggatg
     6841 gccgcccctt tggtgttgag gcgcatagcg ccttccccca cctggcttgc aatcaattcg
     6901 ccttcgctga cgcccagacg atgcgcagca tcgcgcggcc tcagcctgtt ggtggtcaat
     6961 aagtccttcc aaacccgata cagttcaggg ccggatggcc ggttttcggc atcattcccc
     7021 tggatcagct ctgcttcgct caccgctatc cctccagccc gcgacacaaa ggcaaatagt
     7081 cgtcgcatcc catccaagac ataactccgc ttccttatat gttgcaagtg catcgcattt
     7141 gcaattctaa tttggacctg tatatgatta tgagacgcaa tcgcatatcg acaattcaca
     7201 aaccacgaag ggggttatca tgcatcgagt tcgtcaaatc gtcctggctt cggcctgttt
     7261 ggcggcttta ggccatgccg cactcgcacg ggacgcttct gacaaaggac agtatgtaca
     7321 agtggcggcg ctcgagaccg ttacgatttc ggctacgcgc aatcccactt caaccttgaa
     7381 ttactccggc atggtcgacg tgttggacgc cgaggatatt tcggcggcga ttccctcgac
     7441 gatttccgat ctcgtcaaga ccatacccaa cgtgcaattt gtcggcggcc cgcgccggac
     7501 aagcgagagt ccgaacatcc gcggtctcgg cggggaggat gtcctggttc tgatcgacgg
     7561 ggtgcgccag agctggacgt cgggccatga cggccgtttc ttccttgacc cggccctgct
     7621 gtcgtcgatg gaagtcgtgc gcggccccgc ttcggcgctt tatggctcgg gcgccttggg
     7681 cggcgtgatg gccttccgca ccgcggacgc ttcggacttt cttgctccgg gacagacctt
     7741 cggcgcccgg gtggcgttgg gctatcagga tgtggacgaa gaattcctgc gtacggtgac
     7801 agggttcacg cacatcggga atttcgatct catcggcagc atcgggcagc gcagttcggg
     7861 cgatatccgg ctcggctccg gcgccactct ggctgccgac gacgacatcg tgacgggttt
     7921 cgccaaggct ggctacgatt tcggcggcgg cctgagcgcc aaactgtcct accaaggctt
     7981 caagaacgag gcggtcgaac ccgcaaacgg atcggggctt tcgagcgggg cgccggtgaa
     8041 caagaccgtt gtctcgcagc agttcaccgg tcagatcaat tggaaacccg aaggtttcag
     8101 cctcatcgat cttcacctca cgccgtacca tgttcaaacc tcggtcgagg aagccgaccc
     8161 caccaccggc gaacgcacgt ttcgcgacat caagacgaac ggcttgagcc tcgacaaccg
     8221 cacgtctttt tcttttgcca acgtgtcggg cctcttcacc gtgggcggcg actggtacga
     8281 ggacgatcaa gtcggccgcg atacacttgc ccccggcggc gtacgttccg gcgtgcccaa
     8341 tggcagcgac tccttctggg gtatgtttgc gcagatcgag gcgaatatcg atcgcccgct
     8401 cggggcgccc ggcaagctga cgctggttcc ggcaatccgc tacgattcct acagcacctc
     8461 ctcgaccggc aatcccgaca ccgacgaaac ctcggtctcg ccgaaattcg cggcgaccta
     8521 tgcgccggtg aactggctgt tcgcgttcgg caacgtcggc aaagcgtttc gcgcgcccgg
     8581 cgtcaacagc ctctatctga acggcatcca tttctcggtg ccgcatccga tcctgccggg
     8641 cgtatcggtg gccaacacgt ttcagccgaa tccgaccctg aaacccgaaa cctcgcatta
     8701 ctgggaggcg ggtgcaggcg tcgtcttcgc cgacctcatc tccgccggcg attctctgac
     8761 cgcaaaagcg tcctattggg agcagagcgt cgacgattac atctcgctca acgtcttcac
     8821 gccggccacg ttctacgcgc ccggctgctt cacgccgccg accttcctgg cgggttgcaa
     8881 cgtcggctac accacctccc agaacgtcaa tgcgcagctc agcggcgcgg aactgcaagc
     8941 cgcatatgac ggcgagcgcg tccgtctcga ggtcgattac gggaccatga caggccacga
     9001 acgcggcacg ccgtacgacc tcaattcgct gatgcccgat atcgtgacga cggtcgcgac
     9061 cttgaagctt cccgaagccg aggctttact caacgcgcgc gtcacggtgg cggagtcgtt
     9121 caagcgaagc tacaacccgg tgagcaacga tccggcgagc gaaacccgcg agggctatac
     9181 gctgctcgat ctctacgcca cctgggctcc gcgggagctt ctcggcggca agctcaaggg
     9241 cctgcgcgtc gatttcggcg tcgataacgt gaccgacgag gactatacgc cctatgcggc
     9301 gggcgtaagc gccgccggcc gcaatgtgaa ggttctcgcc agctacactt tgggctggtg
     9361 agccgtgttg gccggggccg acattgtgtt gccggtcgag cgacatttcg cccgatcggg
     9421 cgacccgctc gccggcgcat tcgttgaacg cagcgccgga cattgggtgc gtgggcggcc
     9481 cgtgcccgtc cccgatgccg gtgctgcgtt cgcctccctg gtggcggcaa aacggccggg
     9541 caggcgtgcg gcctatctgc atgttccttt ctgcagcaat cactgtttgt actgcggctt
     9601 ttatcggaat cgcgcagacg aggatgcgct cgctgtctac accgcctatg tcgagcgcca
     9661 gatcgcgcgg gatgcggaag cggcgaaggg gttcggaccg gtccatgccg tctatttcgg
     9721 cgggggtacg cccaacgcgc ttgcggccga ggatctgcac cgcctcatcc gtgcggttcg
     9781 ttcgcgactt gccctcgcgc ccgattgcga aatcacggtg gaaggccgcg tgttcggttt
     9841 cacgccggag aaaatcgacg cctgcctggc tgcgggcgcc aaccgcttct ccatcggcgt
     9901 gcagacgttc gatacggacc tgcgccggcg tttcgggcgc aaggctgagc gcgaggaggc
     9961 ggtcgagttc cttcgcgcct tgcgcgaacg cgatgcggcg gcggttgtgt gcgacctcat
    10021 ctacggtctt ccggggcagg atatcgaaag ctggcgccgc gaccttcgaa cctgcatcga
    10081 actcgatctc gacggcgtgg atctctacgc gctgacgcta ttcgacaagg ggccgctggc
    10141 gctctccatc gccaaaggcg cactgccggc cgccgcaagc cagcgcgaat tggctgagtt
    10201 ctatgccgtc ggtctcgaaa tgctggaaga tgcgggctgg cggcacctga cccaggctca
    10261 ttggggatcg gggacgcgcg agcgcaatct ctacaaccag ttcgtcaagt cgggggcgga
    10321 ttgcctggcg ttcggttcgg gagccggcgg gatgctcggc ggccatcgct acatgctcga
    10381 cggcgactgc gaagcttatc agcggcgggt catggccggc gacagaccga tcgccatgat
    10441 gcttgcgccg caaccccacc agcgggtccg cgatctggtg acggccgggc tcgaagcggg
    10501 gcgtctcgat cttgcccggc ttgaagccgc cgccgcgccc ggactctcga aggcgctggc
    10561 gccgctcgtc gatcaatggg cgggggcggg gctggtccgc cgtcgcgcag actcgatagt
    10621 gctcacgacg gcgggctggt tctggcacgg caatctcggc gccagcttga accaattgat
    10681 ttctctctat ctgggcggaa cgcctggaac ggaagtccgt catgtccact gaacccggtg
    10741 tcctgtccgc tctcaaggcg cgtttcgcac aagaccaagg cggagttctg gaagacatcg
    10801 ccgcggaaac cggcctcacg acgcgcgagg tcatggagtg tctcccgccg aactgtcact
    10861 gcgtgccggc cggctattcc ccagatcggg aggttactac caatgaggcc catgcttaat
    10921 ccggagttcc gcacatgaat gcccacaccc cgccgatcgc ctatctcgac gacatgtcgc
    10981 tctctcacct gaacactgcg gaactgctgc tcaccgcgac gctccggctt tatgtcctgg
    11041 cgcagcgtgg agcagagggc gaatgtcccg attggcgcaa cggcatgctg acgacgggcg
    11101 taaggccgtg tgccgtcgtt gctttcgacg ttttggtcaa gaccattctg ggccagacgc
    11161 aacaggcgct cgacgttcgt tgtcccctgt gcccgaagct cggtgcggac gagggccggt
    11221 tcctccagat gacaagcctg ttgcaacagc agcggttcgg ggccgcacac gcgatcttgg
    11281 gccagtggct gccgccgctg gcgcaggctg ccatgatgtc gcccgctgtg catgtggcat
    11341 tcgcatgtgc gagccgcggg ttgcgcgtgc cttggcggca cggcgacacc gaaatgctcg
    11401 cctatttcga cgttccttgc ggcgacccgg gcgtgaactt cgtgcactga cgcttggggc
    11461 cggggtttaa atggcggccc ggataatatg cgtgaacgga aaatcggcgt ggaagcccag
    11521 ccggaagccg agcctccgga caaagcccaa tgcgcgcctg ttgtccgaat ccacaatgcc
    11581 ccataattcc gtaatgcccc gcccggcggc atagtccagg agcaaattgt gcatcaaacc
    11641 gccaaggccg cgcttttgca ggtcggtgcg gacgatcagg gcatattccg cgctcttctt
    11701 gcaggtgtcg ccgacaagac gcgaaaccgc gagaatatcg cctttcaaat tctccgccac
    11761 gaatgccatg tgccgctcat agtcgatctg cgtgagctgc gcggccagct tttcggaaag
    11821 gtggtgaatg ctgcggagga agcggaaacg caaatcctcg gcgctgcaca gcaacgcgcc
    11881 ggcgatgaat ttcggggcat cgtcgggacg tacgggccgt atcagcagtc tttcgccgag
    11941 ccaatcgaca gggcggataa ggtggcttgg gtagctcgtc atcgtccgat caccgccata
    12001 cgatctgtgg accgcgttcc gcgagaagcc caggcagctc gtcgtcataa ggttccggaa
    12061 cgagcgtgag tgtgccgcgg ccttggaaag tctgcaagac ccatcgccgg gtgcccagcc
    12121 cgcccaattc cgccgccctc cggcggcgcc ggctctttcg ccttgcccgg ttttgggcct
    12181 atatcacata tcgagaatgc cttttttgaa gtacgggcga tgcgcccgcc gaagggctat
    12241 cttgaagccg caccggccgg cgaacggcaa ggagggatac cgtgtgtttg gcgattccag
    12301 gccggatcgt gggcgtctcg gacgatgatc cggtcatgcg ggtcggccgg gtcgatttcg
    12361 gcggcatcgt caaggaaatt aacctcgctt atgtgcccga ggcgcgcgtc ggcgaccatg
    12421 tgctcgtcca tgtcggcttt gctctcacgg ttatcgacga agccgaggcc gaccggctgt
    12481 tcgagcaatt gagcgagatt gccgagatcg aagccgcgca aatggaagct acgcagtgag
    12541 acaggccgag gcatatcgcg acggaacact tgcacgggcg ctcgcggcgc gtatcgcccg
    12601 cgaggccggc ccgtccgtcg attacaactt catggagttt tgcggcggac acacccatgc
    12661 catctcgcgc tacggcatag aggatctgct gcctgccaat gtgcgcatga ttcatggtcc
    12721 tggctgcccg gtctgcgtgc tgccggtcgg gcgcatcgac accgcgatcc gcctcgccgg
    12781 acggcccgag gttactttat gcgtctatgg cgatctgatg cgggtgcccg gttcgcacgg
    12841 gtcgtcgctg ctcaaggcca aggccgcggg ggccgatgtc cgcatggttt attcgacgct
    12901 cgatgcgctg cgcatcgccg aagccgaacc ggaacgccaa atcgtcttct tcgccatcgg
    12961 cttcgaaacc acgacgccgc ccaccgccct cgccgtcaag ctcgccgccg cgaagaagct
    13021 cgccaatttc accatcttct gcaaccacgt gctcaccccc tcggcaatgc aaagcatcct
    13081 ggagagcccg gacgtgcgcc tcgacggatt cgtcgggccg gcccatgtct cgacggtgat
    13141 cggcagccgg ccctacgcgt tcgttgcgga agaatacaaa aagcccatcg tgatcgcggg
    13201 cttcgagccg ctcgatgtca tgcaggcgat cctgatgctg gtccgccagg tcaatgaagg
    13261 ccgccacgag gtcgagaatc agtacgcgcg cgcggtcaag cccgaaggca acgaggcggc
    13321 caaggccgaa atggcggaga ttttcgagtt gcgcaaaagc ttcgaatggc gcggcctggg
    13381 ccaagtccct tacagcgggc tgcgtctgaa agagacttat gcggccttcg acggcgagcg
    13441 g
//
LOCUS       DASWPU010000500         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_3959, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000500 DASWPU010000000
VERSION     DASWPU010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3959"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VGC27_10600"
     CDS             complement(<1..472)
                     /locus_tag="VGC27_10600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY0283059.1"
                     /translation="MSHLLSLLRNGDYLTRERARLWAGGFLIAFAAAILFLALTAQGL
                     NDYKNRPLGTDFSNVYAAGTLTRDGVPGTAYDPARQYERERAVFGADTPFYGWHYPPF
                     FLAVADALSALPYLPALILWQGATLALVLLAMARLLRGVQITQGRTWLLYTLAFP"
     gene            complement(537..1673)
                     /locus_tag="VGC27_10605"
     CDS             complement(537..1673)
                     /locus_tag="VGC27_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283060.1"
                     /translation="MPRRPFTFITAALTSAVLVTTPFAPALAGGGSQYGNQGGSRYGN
                     QGGFHGKPASCNNGGSVVVNKPVSINKNINVYKPTTVNNSVNVYKPVTINKDINVYKP
                     TTINKEINVYKPVTINKDVNIYKPITINKDVNITKNIDNSKNITVNKNVNIEKNVNIN
                     KSIVINKGGGGGSAEASAFAQAMASASANASVSNNVIFYGGGNYEYVTVNNRSSDISG
                     IQTTQQCQVQEANVVKAIHAVCVSSDGREFPASHMTGLTWLNSSYEGEVMRCIPGSHL
                     KVVIGDVLQSDQGMVGTYASGQVLECGGREALRHYKDGMLKCAPAVPVPDCTERNNLR
                     LYGTSDMFFSYRASVCVSGAQTASRDLELTGMSLDGGVGDGSGN"
     gene            1860..2567
                     /locus_tag="VGC27_10610"
     CDS             1860..2567
                     /locus_tag="VGC27_10610"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY0283061.1"
                     /translation="MIPISDDNPSRQTPFFTWAIIAVCVAVYIWQVRQGPQSGTVFQL
                     YGFVPKSLMSPPLSPAEETGWSALTNIFTSMFVHGGIWHLAGNMLYLWIFGNNIEDAM
                     GHARFLLFYLLCGVAAALTMAFMDPASTIPMVGASGAISGVLGAYMLLYPRARVTVVI
                     PIVIIFYPFRISAVWVVGVWFAMQLFSAAGSTPDSPGVAWWAHIGGFVAGLLLTPFLK
                     ASHVPFFGPRSLRGPWG"
     gene            2679..>3003
                     /locus_tag="VGC27_10615"
     CDS             2679..>3003
                     /locus_tag="VGC27_10615"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0283062.1"
                     /translation="MKHKNSHLLVGYWSRIRKGREVPDQTDIDPRAIKRMLPYVFILD
                     AEDPSRPVYRLAGTALCERFGFELKGTGFLAHWEAKSGSALASLLRQALRLKQPVCLS
                     SIGASA"
BASE COUNT          496 a          895 c          968 g          644 t
ORIGIN      
        1 ccggaaaagc cagcgtgtac agcagccagg tacggccctg cgtgatctgc accccgcgca
       61 acagacgcgc catcgcgagg agaacgagcg ccagggtcgc gccctgccac aggatcagcg
      121 ccggcagata gggaagcgcg gacagcgcgt ccgcgacggc caggaagaaa ggcggatagt
      181 gccagccata gaagggcgta tcggcgccga agaccgcccg ttcccgttcg tactggcgcg
      241 cgggatcgta agcggttccg ggcacgccat cgcgcgtcag cgtgccggcc gcgtagacgt
      301 tggagaaatc ggtgcccagc ggtcggttct tgtagtcgtt cagcccctgt gcggtcaggg
      361 ccaggaatag gatcgccgcc gcgaaggcga tcaggaatcc gcccgcccac agacgcgcgc
      421 gctcgcgggt gaggtaatcg ccgttgcgca acagggatag aagatggctc aacgcccggc
      481 cttcatgcgg ctttgcgcgc gcatgttggg ccggaggcgt ttaaggaatg atttctctag
      541 tttccgctgc cgtcgccgac gccgccgtca aggctcatgc cggtgagttc gaggtcccgc
      601 gaggccgtct gggcgccgct gacgcagacc gaggcccgat aggagaagaa catgtcgctg
      661 gtaccgtaga ggcgcagatt gttccgctcg gtgcagtctg gaaccggcac ggcgggcgcg
      721 catttaagca tgccgtcctt gtaatggcgc agcgcctcgc ggccgccgca ttccagcacc
      781 tggccgctgg cataggtgcc gaccatgccc tggtcgctct gcagcacgtc gccgatcacg
      841 accttgagat gcgaaccggg gatgcagcgc atcacttcgc cctcatagga ggaattgagc
      901 caggtcaagc ccgtcatgtg cgaggcgggg aattcgcgcc cgtccgacga cacgcagacg
      961 gcgtggatcg ccttgacgac gttggcttcc tgcacctggc actgctgggt ggtctggatg
     1021 ccggaaatgt cgctgctgcg attgttgacg gtcacatatt cgtagttgcc gccgccgtaa
     1081 aagatgacgt tgttgctcac gctggcgttg gcgctggcgc tcgccatggc ttgcgcgaag
     1141 gccgaagctt ccgccgagcc gccgccaccg cccttgttga tcacgatcga cttgttgatg
     1201 ttgacgtttt tctcgatgtt aacgttcttg ttgaccgtga tgttcttcga attgtcgatg
     1261 ttcttggtga tgttgacgtc tttgttgatg gtgatcggct tgtagatgtt gacgtccttg
     1321 ttgatggtga cgggtttgta gacgttgatt tccttgttga tcgtcgtcgg cttgtagacg
     1381 ttgatgtcct tgttgatggt gaccggctta tagacgttga cgctgttgtt gaccgtcgtc
     1441 ggcttgtaga cgttgatgtt cttgttgatc gagaccggct tgttgaccac gactgagccg
     1501 ccgttattgc aagaggccgg tttgccgtgg aagccgccct gattgccgta gcgggagccg
     1561 ccttgattgc cgtattggga gccgccgccg gccagggccg gggcaaaggg ggtcgtcacc
     1621 aagacggcgc tggtcagcgc ggcggtgatg aaggtgaaag gcctgcgcgg cattgctatt
     1681 gctccttcgc tccggtcatc ctatgcgtcc aatgcccggg agaacgccca ggacatggaa
     1741 caagccttaa tgaccgcccg tcccgcttca cgaaatatgc caacctgcgt cttgagattg
     1801 cccgcttctc cggctcgaat tggcacaggt ccggggctag gctggaccgg ggaagagcca
     1861 tgatcccgat ctcggacgac aatccgtcgc ggcaaacgcc gtttttcacc tgggcgatca
     1921 tcgccgtctg cgtggccgtt tacatatggc aggtgcgcca aggccctcaa tcggggacgg
     1981 ttttccagct ctacggcttc gtgcccaagt cgctgatgtc gcctccgctg tccccggcgg
     2041 aagagacggg ctggtccgcg ctcaccaaca tcttcacctc gatgttcgtg catggaggca
     2101 tctggcacct cgcaggaaac atgctttatc tctggatttt cgggaacaac atcgaagacg
     2161 ccatggggca tgcgcgcttc ctgctgttct atctcttgtg cggcgtggcg gcggcattaa
     2221 ccatggcttt catggatccg gcgtccacca tcccgatggt cggcgcctcg ggcgcgattt
     2281 ccggcgttct cggcgcctat atgctgcttt acccccgggc gcgggtcacg gtggtcatcc
     2341 cgattgtgat catcttctat cccttccgca tcagcgccgt gtgggtcgtg ggcgtttggt
     2401 tcgccatgca gcttttcagc gcagcgggca gcacgcccga cagtcccggc gtcgcttggt
     2461 gggcgcatat cggcggtttt gtagccggat tgctgctcac gccgttcctg aaagcttcgc
     2521 acgtcccctt cttcgggccg cgcagtttgc ggggcccctg gggctgacgc agggacattg
     2581 cacgggtttc gctctcccgt cgttccgaga acgcgacatc gtttcgattt ggatcaagac
     2641 ggaacggtcg ctcgacggaa caacgagacg ggcgggtcat gaaacacaag aacagtcatt
     2701 tgctggtggg gtattggagc aggatccgca agggccgtga ggtccccgat caaaccgaca
     2761 tcgatccgcg cgcgatcaag cgcatgctgc cctatgtgtt catcctggac gccgaggacc
     2821 cgtcgcggcc ggtttaccgt ctggcgggca ccgcgctttg cgagcgtttc ggctttgagc
     2881 tcaaaggcac cggctttctc gcccattggg aagcgaaatc gggcagcgcg ctcgcttcgc
     2941 tgctcaggca ggcgctccgg ctgaagcagc ccgtttgcct gtcctcgatc ggcgccagcg
     3001 ccg
//
LOCUS       DASWPU010000501         4688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_89864,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000501 DASWPU010000000
VERSION     DASWPU010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89864"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /gene="polA"
                     /locus_tag="VGC27_10620"
     CDS             <1..1642
                     /gene="polA"
                     /locus_tag="VGC27_10620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY0283063.1"
                     /translation="DLGGSDDIVQMDEADVLGRLKPLLEDPSILKIAQNLKYDFLIFQ
                     QRGIRVAPYDDTMLMSYVLDGGLHGHGMDELSEIYLKHTPISFADVAGKGKEKVTFDR
                     VPIPEATRYAAEDADVTLRLWLLLKPRLAEEKKRTVYETLERPLAPVLAEMEREGIAV
                     DPDLLRKLSNDFATDMAKLESEIHKLAGESFNLASPKQLGEILFDKFSLSGGKKTKTG
                     AWSTDADALEELAAQGHDLPRKVLDWRLLAKLRGTYTDALPTYINPQTGRVHTSFAMS
                     AAATGRLASTDPNLQNIPIRTPEGRRIRQAFIAPKGSKLISADYSQIELRLLAHIADI
                     AALKKAFADGLDIHAMTASEIFGVPVKDMPPEIRRRAKAINFGIVYGISAFGLANQLG
                     IPRSEADDYIKKYFQRFPGIRDYMEAAKAYAREHGYVETLFGRRIHIREIKSANQGFR
                     GGAERAAINAPIQGAAADIIRRAMIRMPGALAAAKLTAKMLLQVHDELVFEAVDAEVE
                     KTCSLAASVMEGASLPAIPLSVKLTVEARAAQNWDAAH"
     gene            1687..2643
                     /locus_tag="VGC27_10625"
     CDS             1687..2643
                     /locus_tag="VGC27_10625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HEY0283064.1"
                     /translation="MSDQPTELQDEFHEHIETIRRFNRLYTRRIGLLQKAHLGSPFSL
                     TEARVLFELAHRASLTARDLCDLLALDQGYVSRILARFQKRGLISKRASAEDARAQCI
                     ALTASGRKSYAALDLRTRTAIAELLEGKPEAVQRDIAEAARTLERLLGEAPQKPAPLV
                     IRPPKPGDLGWIVHRHGVLYAREYGWDASFEALVAEIVADFGKKADAARERCWVAERD
                     GEILGAVFLVKDDDQTARLRLLYVEPAARGQGLGRQLVELCVAFARDAGYWKIVLWTH
                     DVLTAARKIYQKAGFRLVNRDAHRAFGKNVVSENWALDLAQS"
     gene            complement(2656..4395)
                     /locus_tag="VGC27_10630"
     CDS             complement(2656..4395)
                     /locus_tag="VGC27_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283065.1"
                     /translation="MAKQIHFEIFSRRGSKGGWKLIDVKPGRDEAVKLAQEMMREGGA
                     TGVKVVKETYDEVSRDFLSLKVFEDGHNKLKSTPAQENTPSALPCFKPDDFYSYHARK
                     TIARLIPEFLARNKITVTELCHRADMLEKLDATGTLLQHAIQKIAVAQAAATTTPVQQ
                     IVKNLNALTAKAFHRVYHDDRKGVFPGPVAGKFGALAAGLAERSNGLYLLNGAIARHL
                     RDAKSWDEKVSRLLALMEESPADEPGGALLLSAIDTIIAEILSGSAALRELIGDKDNL
                     GTALTELVQLFLGKKPKAQDGAQQGLNLLTERFAADDLPEARTAIAHRLIAEFRSTKR
                     LCPESLVDELKALRQIANLVVQGVGKYLSHDDLIAAFTLRSHRLVNQVTLSEHLADAA
                     TPGEKLERLLFIEENIIGAENKRRLAVFVKAIVAAGEFENHFHAPNVAVLTRLQRLAA
                     LNARVRRSGFQDNQRMEIADRLDLVACNVEARVKLFESLEAKSANPAEKAGIVLRLFT
                     AGSFTEPRLAAKARALIVGHLSKPGFLSGYVAQVARPGEQKLDADAAIAELMQMLEKT
                     GISRETGLKSIAA"
     gene            complement(4454..>4688)
                     /locus_tag="VGC27_10635"
     CDS             complement(4454..>4688)
                     /locus_tag="VGC27_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY0283066.1"
                     /translation="CSETGVLGAAAGVMGTLQALEILKEISGTGESMAGRLLVFEALE
                     TRFRTVTVRPDPACRLCGPNPSIHPLAQRERRV"
BASE COUNT          862 a         1489 c         1478 g          859 t
ORIGIN      
        1 cgacctcggc ggcagcgacg acatcgtgca gatggacgag gccgatgtgc tggggcgcct
       61 gaagcccctg ctcgaagacc cctcgatcct caagatcgcg cagaatctca aatacgattt
      121 cctgatcttc cagcagcgcg gcatccgcgt cgcgccttac gacgacacga tgttgatgtc
      181 ctatgtcctc gacggcggcc tgcacggcca cggcatggac gagctgtcgg agatatatct
      241 gaagcacacg ccgatttcct tcgccgacgt cgccggcaag ggcaaggaaa aggtcacctt
      301 cgaccgcgtg ccgatccccg aggcgacgcg ctatgcggcc gaggacgccg acgtgacgct
      361 gcggctgtgg ctgctgctca aaccgcgtct ggcggaggaa aaaaagcgca cggtttacga
      421 gacgctggag cggccgctgg cgccggtcct ggccgagatg gagcgcgaag gcatcgcggt
      481 cgatcccgat ctgttgcgca aactgtccaa cgatttcgcc accgacatgg ccaagctcga
      541 aagcgaaatc cataagctcg cgggcgaaag cttcaacctc gcctcgccca agcaattggg
      601 cgaaattctg ttcgacaaat tctctctctc cggcggcaag aagaccaaga ccggcgcctg
      661 gtccaccgac gccgacgcgc tggaggagct ggcggcgcaa ggccacgacc tgccccgcaa
      721 agttctcgac tggcgcttgc tcgccaagct gcgcggcacc tataccgacg cgctgcccac
      781 ctacatcaat ccacaaacgg ggcgcgtgca tacctccttt gcgatgtccg ccgccgcgac
      841 cggccggctg gcctccaccg atcccaattt gcagaacatc ccgatccgca ccccagaagg
      901 gcggcgcatc cgccaggcct tcatcgcgcc gaagggatcg aaactcatca gcgccgatta
      961 cagccagatc gaattgcgcc tgctggccca catcgccgac atcgcggcgc tgaagaaagc
     1021 tttcgccgac ggcctcgaca tccacgccat gaccgcctcg gagattttcg gggtgccggt
     1081 caaggacatg ccgccggaaa tccgccgccg cgccaaggcg atcaatttcg gcatcgttta
     1141 cggcatttcc gctttcggtc tcgccaacca gctcggcatc ccgcgcagcg aggccgacga
     1201 ttacatcaag aaatatttcc agcgcttccc cggcatccgc gactatatgg aagcggccaa
     1261 ggcttatgcc cgcgaacacg gctatgtcga aaccttgttc ggccggcgca ttcacatccg
     1321 cgaaatcaaa tcggccaatc agggttttcg cggcggagcc gaacgcgccg cgatcaatgc
     1381 gccgatccag ggcgccgccg ccgacatcat ccgccgcgcc atgatccgca tgccgggcgc
     1441 gctggcggcc gcgaagttga cggccaagat gctgctgcaa gtacatgacg aactggtgtt
     1501 cgaagcggtc gatgccgagg tcgagaaaac ctgctcgctg gcggcgtcgg tgatggaagg
     1561 ggcctcgctg ccggcgatcc cgctcagtgt caagctgaca gtggaggcgc gcgccgccca
     1621 gaactgggac gcggcccatt gagttagtgc ttgacatagt caagtatttg catgactata
     1681 gcatgtatgt cagatcaacc gactgaactg caagacgaat tccacgaaca catagagacc
     1741 atccgccgct tcaatcggct ctacacacgc cgcatcggcc tcttgcagaa agcccatctg
     1801 ggcagcccct tttcgctgac cgaggcgcgc gttctgttcg aactggcgca tcgggcgtcg
     1861 ctcaccgccc gggatttgtg cgaccttctt gctctcgacc aaggctatgt cagccgcatc
     1921 ctggcgcgct tccagaagcg aggattgatc agcaagcgcg cgtcggctga ggacgcccgc
     1981 gcccagtgca tcgcgctcac cgcgtcgggg cggaaatcct acgcggctct cgatctgcgg
     2041 acacgcaccg ccatcgccga attgctggag ggcaagccgg aggccgtcca gcgcgacatc
     2101 gccgaggccg cgcggacgct ggagcgcctg cttggagagg cgccgcagaa gcccgctccg
     2161 cttgtgatcc ggccgcccaa gcccggcgat ctgggctgga tcgtccatcg gcacggcgta
     2221 ctctacgcgc gcgaatatgg ctgggatgca agtttcgaag cactggtggc ggaaatcgtc
     2281 gccgatttcg gaaaaaaagc cgatgcagcc cgcgaacgct gctgggtcgc cgagcgcgat
     2341 ggagaaattc tcggggccgt ttttctggtg aaggacgacg accaaacggc acggctgcgc
     2401 ctgctctacg tcgagcctgc ggcgcgcggc caagggttgg ggcggcaact ggtcgagctt
     2461 tgcgtcgcct tcgcccgcga tgcgggttac tggaaaatcg tgctgtggac ccacgacgtt
     2521 ctaacggcag cgcggaaaat ctatcaaaaa gcgggcttcc ggctggtgaa tcgggatgcg
     2581 caccgcgcct tcggcaagaa cgtcgtcagc gagaactggg cactggacct cgcgcaatcc
     2641 tgaggcgggc gcggttcacg ccgcgatgga tttcaggcct gtttcccggc tgatgccggt
     2701 tttctccagc atctgcatca gttccgcgat cgccgcgtcc gcgtcgagtt tttgctctcc
     2761 gggcctggcg acctgggcga cgtagccgct gaggaagccg ggtttggaca gatgaccgac
     2821 gatcagcgcg cgcgccttgg cggccagccg gggctcggtg aagctgccgg cggtaaacag
     2881 gcgcaggacg atgcccgcct tttcggcggg gttcgcggac ttggcctcca gggactcgaa
     2941 caatttgacg cgggcctcga cgttgcaggc gaccaggtcg agccggtcgg cgatctccat
     3001 gcgctggttg tcctgaaagc cggagcggcg cacccgggcg ttcaacgccg ccaggcgctg
     3061 cagccgggtc agcaccgcaa cgttcggcgc gtggaaatgg ttctcgaatt cccccgccgc
     3121 aacgatcgct ttgacgaaga cggcaagccg gcgcttgttt tccgccccga tgatgttttc
     3181 ctcgatgaac agcagccgtt cgagtttctc tccgggcgtc gccgcgtcgg ccaaatgttc
     3241 gctcaaggtc acctgattga ccagccgatg ggagcgcagc gtgaaagcgg cgatcagatc
     3301 gtcgtggctc agatatttgc cgacgccctg caccacgagg ttggcgattt ggcgcagcgc
     3361 cttgagttcg tcgaccagag attcggggca gaggcgtttg gtgctcctga actcggcgat
     3421 cagccgatga gcgatggcgg tgcgcgcctc cggcagatcg tccgcggcga agcgttcggt
     3481 cagcaagttc aatccctgtt gcgcaccgtc ttgcgccttc ggcttcttgc cgaggaaaag
     3541 ctgcaccagc tccgtcaggg cggtgcccag attgtccttg tcgccgatca actcgcgcaa
     3601 ggcggccgag ccgctcagga tttcggcgat gatcgtgtcg atcgccgaaa gcagaagcgc
     3661 gccgcccggc tcgtcggccg gtgattcctc cattaaggca agcaagcgcg agactttctc
     3721 gtcccagctt ttggcgtcgc gcagatggcg cgcgatcgcg ccgttgagca gatagagacc
     3781 gttcgagcgc tcggccagcc cggcggccag ggcgccgaat ttccccgcca ccgggcccgg
     3841 gaagacgcct ttacggtcgt cgtgatagac gcggtgaaag gctttggccg tcagcgcgtt
     3901 gagattcttg acgatctgct ggacgggtgt cgtcgtggcg gcggcctgcg ccaccgcgat
     3961 tttctggatg gcgtgctgca gcagcgtgcc ggtcgcatcc agcttttcca gcatgtcggc
     4021 gcgatggcag agttcggtga ccgtgatctt gttgcgcgcg agaaattcgg ggatgaggcg
     4081 ggcgatcgtc ttgcgggcgt gataggaata gaaatcgtcg ggcttgaagc agggcagcgc
     4141 ggacggcgta ttctcctgcg cgggcgtgga tttgagcttg ttgtgcccat cttcgaacac
     4201 cttcagcgag agaaagtcgc ggctcacctc gtcgtaggtt tccttgacca ccttgacccc
     4261 ggtcgcgccg ccttcgcgca tcatctcctg cgccagcttg accgcttcgt cgcggccggg
     4321 tttgacgtcg atcagtttcc agccgccttt ggaaccgcgc cgcgagaaaa tttcgaaatg
     4381 gatttgcttc gccatgcacc ccttgcccgc atcgaattgc ggacagtatg tggagcgggg
     4441 atttaagcag cggttaaacg cggcgttcgc gctgcgccag aggatggatc gacggattgg
     4501 gtccgcacaa acggcaggcc ggatcggggc gcactgtgac agttcgaaaa cgagtttcca
     4561 gtgcttcaaa aacgagcagc cggcccgcca tgctctcgcc ggttccggaa atctctttga
     4621 gaatttcaag cgcctgaagc gtgcccatga caccggcggc ggcgcccagt acgccggttt
     4681 cgctgcac
//
LOCUS       DASWPU010000502         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_89901,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000502 DASWPU010000000
VERSION     DASWPU010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89901"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(613..689)
                     /locus_tag="VGC27_10640"
     tRNA            complement(613..689)
                     /locus_tag="VGC27_10640"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(653..655),aa:Met,seq:cat)
     gene            1077..1493
                     /gene="dksA"
                     /locus_tag="VGC27_10645"
     CDS             1077..1493
                     /gene="dksA"
                     /locus_tag="VGC27_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY0283067.1"
                     /translation="MGTQLPSGYKPSESEPFMNERQREYFRRKLLSWKEEILRESRET
                     IQNLQAETVPHADLADRASTEAERQLELRTRDRQRKLIAKIDAALRRIEDGSYGFCEE
                     TGEPISLKRLDARPIATLSIEAQERHERREKVYRDD"
     gene            complement(1558..>1873)
                     /locus_tag="VGC27_10650"
     CDS             complement(1558..>1873)
                     /locus_tag="VGC27_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY0283068.1"
                     /translation="DTSNVGGGSVDRSEKINLTLAALVTQVLPNGNMVIAGHQQMRVN
                     GELRDLQVSGIVRTEDITSANMIDLTQIAEARISYGGRGTVSDMQQPRLGSQLFDILM
                     PW"
BASE COUNT          388 a          571 c          547 g          367 t
ORIGIN      
        1 ggcgccattc agttcgatcc ggattcgcct cggtctctgg gcaagaacgc ggacggcgcc
       61 atcgcgcgcg cctaccgcct ccatcatcgc gacgattacg gcagcttcta cgttccgcgc
      121 tgagcgctca agcgcgccgc gataaaacgg gcatctccta ccgttacgtt gcggtagatg
      181 tttcgggaga ttgcaaaaat ctcgacggcg gaagcgccgt taacatgcca tcgcgatgca
      241 ctggaaatgc aggcggtgcg ccgccgcgct acctttctgg cggcgatacg agcaaggaac
      301 agtcattcgc aactcgtgtg attggggtga actctacaca cgaataccgc agccagaaat
      361 cgccggggtg agcgtggata ccatcgaagt caataaaacg gctgatcgag cgaggcgagc
      421 gatctccacc ttcaaagcag tgctttggac cgctggcgtc agtgacggct cttagcagat
      481 cacagtgaac tcctggtcgt gttcacacgc taacggcatg tgaacatcag taataagcaa
      541 ggctgtcacg gtcgcctttt cgtgatctaa ataattgatt atatttgaat ttacagtgat
      601 ttatgttgga attggttgcg gggacaggat ttgaacctgt gaccttcagg ttatgagcct
      661 gacgagctac cggactgctc caccccgcga taccgccttt ttcgccgtgt ccggcgaagg
      721 gtgcgcggat ataggacatc gcacccaccc tgtccactcc cccgcttttt gccccttccc
      781 ggcacgcaca ggccgcgccg gaaccagccg gcaggcgatg agttgcaccc aggaggcgcg
      841 gtccgaggcg gcaaagcgcc ttgtccggcc ggtatcttgc ccagcgccgg ccagtctccc
      901 cgggctgcac aacgccgcag ggtcaacctt gagcgtccgc cgtgcggtaa ggtctaagtt
      961 gttgattgac agaaatattt caacttaacg ccgtcacggg attccgattg atgcgggagg
     1021 gggcggccgc tataaccccg gcccacgagc ggcctcacct tgcgaaggga gtttgcatgg
     1081 gtacccaact tccgtccggc tacaagccgt cggaaagcga accctttatg aacgagcggc
     1141 agcgcgaata tttccgccgc aagcttctca gctggaaaga agagattctt cgcgaaagcc
     1201 gggagactat ccagaacctt caggccgaga ccgtgccgca tgccgatctt gccgaccgcg
     1261 cctccaccga agcggaacgc cagctcgaac ttcgcacccg tgaccgtcag cgcaagctga
     1321 tcgccaagat cgatgcggcg ctgcgccgga tcgaagacgg gtcctacggc ttttgcgaag
     1381 aaaccggcga accgatttcg ttgaagcggc tcgacgcccg cccgatcgcc accctgtcga
     1441 tcgaggccca ggaacgtcac gaacggcgcg aaaaagtcta tcgggacgat tgaccagacg
     1501 cgaatagcga atagcgagtg gccctattcg cacctcgcca ttcgctattc gctcttttta
     1561 ccacggcatc aggatgtcga agagctggct gccgagccgc ggctgctgca tgtcgctgac
     1621 cgtgccgcgc ccgccatagg agatgcgcgc ttcggcgatc tgggtcaggt cgatcatatt
     1681 ggcgctggtg atgtcttcgg tgcgcacgat acccgagacc tgcaaatcgc gcagttcgcc
     1741 gttgacgcgc atctgctggt gaccggcgat caccatattg ccgttgggca gcacctgagt
     1801 gaccaacgcg gccagcgtca gattgatctt ctccgagcgg tcgacggaac cgccgccgac
     1861 gttcgaggta tcc
//
LOCUS       DASWPU010000503         4766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_66127,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000503 DASWPU010000000
VERSION     DASWPU010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66127"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1220)
                     /locus_tag="VGC27_10655"
     CDS             complement(81..1220)
                     /locus_tag="VGC27_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0283069.1"
                     /translation="MGKKQAMKTCPICGGGSLAVTIERPRLPVLQNRVYATLEAALQS
                     PCAPFKLATCTTCGFTFNSAFNSNLVVYDETYDNDVPSPTFSKYYETLARQFMERFAL
                     RSGTVIDIGCGKGAFLRTLCRLVPGIKGIGIDPSCTPISEGNCTLIQDIFRPQYFGPD
                     AKLVLLRHVLEHLADPVQFLEMLRRASPNAPLIVEVPDLDWIFRRGAFWDFCYEHSNY
                     FTAQSLAYAANKAGFSVEHHEYSFGQQYQWAICTPSEGDPAFPDAATAVANARGYAKS
                     EAALLSLTGARIRKAETCVLWGMATKGVIFAGLMNSDRILGGIDVNTKKQERFVPGSG
                     LKVHAPEWLKTLKGSPAVIVMNENYLDEIRATITAMGLRLQAEAV"
     gene            1382..2146
                     /locus_tag="VGC27_10660"
     CDS             1382..2146
                     /locus_tag="VGC27_10660"
                     /inference="COORDINATES: protein motif:HMM:NF016850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HEY0283070.1"
                     /translation="MKVNIDTSAKTISIGDETGSKQFDLFSAESFDALSRLWIDIGWV
                     RKYPYAFSWLGRPIIQLPEDIIRAQEVIYRVKPDVILETGVAHGGSLVFYASLCKAIG
                     NGRVIGVDVEIRPRNRAAIEAHELFSYITLIEGNSVAMETVDKVKSLIKPGETVMVIL
                     DSSHTKSHVLAELGAYAGLVTKGSYICVEDGVMHYVAGMPRTKDDWAWNNPKSAAEEF
                     AKEHPEFEIVSLPRGFDESQGVPDCTHHPSGWLLRR"
     gene            2153..3052
                     /locus_tag="VGC27_10665"
     CDS             2153..3052
                     /locus_tag="VGC27_10665"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0283071.1"
                     /translation="MQLLVTGPGGFIGRSLGPFLNRYEVHLADRSDPGWPGKATFHTV
                     DLLRQGGAKDLIERVKPDCLLHLAWYADAGKFWHAPQNLNWLTSSLELFTAFAENGGK
                     RMVVAGTCAEYDWSYNPAVETTPCNPKTLYGKTKAALFSRLQTLSEQSGVEFAWGRIF
                     FPYGPHEQYGRLVPDVITGLLMNKPVSCSSGWQIRDFMHVEDVARAFSVVLKSDLRGP
                     INIASGEGHTVREVATILAEIIGHPERLAFGDRALQPGEPPALVADTALLQSLGFRPR
                     FALRQGLEDAVQWWRAVVSTSNG"
     gene            3291..>4766
                     /locus_tag="VGC27_10670"
     CDS             3291..>4766
                     /locus_tag="VGC27_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0283072.1"
                     /translation="MNVLRLNIISNVAGKLWNILIGLAFIAIYLRLLGAEAYGLVAFF
                     TMLLSTLQILEFGLGTTINRELARYSISSKNARDARDLSRTMEEIYFLVAICIGGALV
                     LAAPALATGWLRVQTLPLSTVTTAVAEMGIAIALLWPCSLYNNGLIGLERQALQNIIG
                     TVLYTLRTVGAVGALIFFGRSIEIYFLWQIIFGFVQLAVLAFFFWRFMPHSPERPRFQ
                     FAQLRRIWRFTLGLGATGLVSFVLSQMDKVLLSRMLPLVLFGYYGVANQFNIASRMLP
                     NALFTAFFPRFSALIAQGNETEVRRLYHRGCQFVSLVVFPAAMTGVFFASQVIRIWTG
                     SPQIAQAAGPIASVLLLGSAFNSVMGTPYNVTVARGWTSFGFYNGLVSAIFFGPVIVV
                     LVTAYGGLGAAIAWALLNCGYLAISAPIIHARVLRGELKTWYLTDVGSGFLASTAVPC
                     IVKLALPADISPWLTIVCVGSTWAASTICCALALPETRQFTV"
BASE COUNT          960 a         1330 c         1366 g         1110 t
ORIGIN      
        1 atggatacct tgcgcgaaaa gagatatctg gaagcgctct gggcgtccgg aaaggcgcct
       61 tgggcgaagc ccgcaaccgg ctagaccgcc tcggcctgca accgaagccc catggccgta
      121 atcgtcgccc gtatttcatc gagatagttt tcattcatta cgatgactgc gggcgagcct
      181 ttaagcgttt tcaaccattc gggcgcatgc actttcaaac ccgacccggg gacaaagcgc
      241 tcctgttttt ttgtattcac gtcgatcccg cccagaatgc ggtccgagtt catcaaaccg
      301 gcgaagatca cgccctttgt cgccattccc cataggacac aggtttccgc ttttcgtatg
      361 cgggcaccgg tcagggagag caatgccgcc tcggacttcg catagccccg agcattggcg
      421 actgcggttg ccgcatcggg gaaagccgga tcgccttcgg atggcgtgca tatcgcccat
      481 tgatattgct ggccgaacga atattcgtgg tgttcaacgg aaaatcccgc tttgttcgcc
      541 gcataggcga gggattgcgc cgtgaagtaa ttcgaatgct cgtagcagaa atcccagaag
      601 gcgccacgtc ggaatatcca atcgaggtcg ggtacttcga cgatcaacgg agcattaggc
      661 gacgcccttc gcaacatttc aaggaactga accggatcgg caagatgttc cagcacatgc
      721 cgcagaagaa ccagcttggc gtccgggccg aaatactgag gacgaaatat gtcttgaatg
      781 agagtgcagt tgccttcgga aatcggtgta cagctcgggt cgattccgat gcccttaatt
      841 ccgggcacga gcctgcagag cgtacgcaga aacgcccctt tgccgcatcc gatgtcgatg
      901 accgtccccg atctaagagc aaaccgttcc atgaattggc gtgcaagcgt ttcgtagtat
      961 ttggaaaatg tcggggatgg cacgtcgttg tcgtaggttt cgtcgtatac gaccaaattc
     1021 gaattgaacg ccgagttaaa cgtgaagccg catgtggtgc aggtcgcaag cttgaaaggc
     1081 gcacagggcg attgcagagc ggcttcgaga gtggcgtaga ccctgttctg caagaccggc
     1141 aggcgcggac gttcaatggt gaccgcgaga gaaccaccgc cacatatggg gcaagttttc
     1201 atcgcttgct tctttcccat ctcaaaattc aaaaactcgg cactatcgcc aaccgtacat
     1261 gccccatcct tcgcccgttc ggcgcggtga acgacggtga taattcgctt gacagtggtc
     1321 gttaatcaag aaactttgtc gctcaaagac cggattcaaa tgacgaaagc gcagagctat
     1381 tgtgaaagtc aacattgaca cttccgcgaa gactataagc attggggatg aaacggggtc
     1441 caaacaattc gatctcttct ccgcggaaag cttcgatgcg ctctcaagat tatggattga
     1501 tataggctgg gtccggaaat acccttatgc tttcagctgg ctcggccggc ccatcatcca
     1561 actgccggaa gacatcattc gagctcagga agtgatttac cgggtaaaac ccgatgtaat
     1621 cctcgagacg ggtgtcgcgc atggcggctc gttggtgttt tacgccagcc tgtgcaaggc
     1681 aatcggcaat gggcgggtga ttggcgtcga cgtggaaatc cggcctcgta accgcgccgc
     1741 catagaagcg cacgagcttt tctcctacat caccctaatc gaaggaaact ccgtggcgat
     1801 ggaaacggtt gataaggtca aatcccttat taaacccggg gaaacagtca tggtgatatt
     1861 ggattccagc catacaaaat cgcatgtgct ggcggaactc ggcgcctatg ccggcctggt
     1921 cacgaagggc tcctatattt gtgtcgagga tggcgtgatg cattatgttg cagggatgcc
     1981 gcgcacgaag gacgattggg cttggaacaa ccccaagtcg gcggccgaag agttcgcgaa
     2041 agaacacccg gagtttgaga tcgtgtcgct gccgcgcggg ttcgacgaaa gccagggcgt
     2101 tcccgattgc acccaccacc cgtctggctg gcttttgcgg cgctgagatc aaatgcagct
     2161 tcttgtcacc ggacctggtg gattcatcgg cagatcgctc ggtccgtttc tgaaccgtta
     2221 cgaggttcat ctagcggacc gttccgaccc tggctggccc ggtaaagcga cattccatac
     2281 cgtggacctg ttgcgacagg gcggcgccaa ggatttgatc gagcgcgtga agcccgactg
     2341 cctgctccat ctcgcatggt atgcagacgc cggaaaattc tggcatgcgc cccagaattt
     2401 gaactggttg acgagttctc tggaactttt cacggcgttt gcggaaaacg gcggcaagcg
     2461 gatggtcgtc gcaggaacct gtgccgagta cgattggagc tataacccgg cagtcgaaac
     2521 gacgccgtgc aatcccaaaa cactctacgg caaaacaaaa gccgcccttt tttcgcgtct
     2581 ccaaactcta tccgaacaat cgggcgtcga attcgcctgg gggcgcatct tttttcctta
     2641 cggtccgcac gaacagtatg gccggcttgt gccggacgtg atcaccggcc tgctgatgaa
     2701 taagccagtt tcctgttctt cgggttggca aatacgggac ttcatgcatg tcgaagacgt
     2761 tgcgcgcgca ttctccgtcg tgctgaaaag cgatctgcgt ggtcccatca acatcgcatc
     2821 gggggagggc cataccgttc gagaggtggc gacgatattg gccgaaatta tcgggcaccc
     2881 ggaacgtctg gcgttcggcg atagggcatt gcaaccgggc gagccgccgg cgcttgtggc
     2941 cgataccgcg ttgctgcagt ctctcggttt caggccacgc ttcgccttgc gacagggcct
     3001 tgaggacgct gtccagtggt ggcgggcggt ggtaagcact tcgaacgggt aaatccgaca
     3061 ttacctgcga ggattgttca aaggcgtggg atcaatagcc gtggatttgg actatacgat
     3121 cttgcctgaa ttgggggggc cgccttgccg ccggcaggtt tgatcttgac ggacgaatct
     3181 atgatcgcgc cctcgctagc aggccgcaac gccatctggc ggctagccat ttcctatctg
     3241 gatcgggaaa ctctcacgcg accggaccac gccggagaga tggtttcccc atgaacgtgt
     3301 tgaggctaaa cattatttcc aacgtcgcgg gaaaactttg gaacatcctg atcggtttgg
     3361 cgtttatcgc gatctatctg cgccttctcg gtgcggaggc atacggactg gtcgcgtttt
     3421 tcacgatgct gctgtcgacc cttcaaatcc tcgaattcgg cctcgggacg acgatcaacc
     3481 gcgagcttgc gcgttattca atcagcagta aaaacgctcg cgatgcgcgc gacttaagcc
     3541 ggacgatgga ggaaatttat ttcctggtgg cgatctgcat cggcggcgcg ctcgtcctgg
     3601 ccgcgccggc tcttgccacc ggttggcttc gcgttcaaac gctgccgtta tcgacggtga
     3661 ccaccgcggt ggcggagatg ggtattgcca tcgcgctgct ttggccatgc agcctctaca
     3721 acaacggcct catcggcctg gaacgccagg cgttgcagaa cattatcggc accgtgctct
     3781 ataccttgcg cacggtgggc gccgtcggcg cattaatttt tttcggccgc agtatcgaga
     3841 tatattttct ctggcaaatt atttttggtt tcgtccaact ggccgttctc gccttctttt
     3901 tttggcgctt catgccgcac agcccggaac ggccgcggtt tcagttcgcc cagttgcggc
     3961 gcatatggcg cttcacgctc ggactgggcg cgaccggcct cgtgagcttc gtcctttcgc
     4021 agatggacaa ggtcctgctc agccgcatgc ttccccttgt tctcttcggc tattacggcg
     4081 tcgccaacca gttcaacatc gcgtcgcgca tgcttccaaa cgcgctcttc acggcgtttt
     4141 ttcctcgctt ttccgccctc atcgctcagg gcaacgagac ggaagttcga aggttgtacc
     4201 atcgaggctg ccagttcgtg tcgctggtgg tgttccccgc cgccatgacc ggcgtctttt
     4261 ttgcgtcgca ggttattcgt atttggaccg gaagccccca aatcgcacag gcggcagggc
     4321 cgatcgcgtc ggtcctgttg cttggctcgg cgttcaacag cgtgatggga acgccctata
     4381 atgtcacagt cgcccgtggc tggaccagtt tcggtttcta taacggtctt gtctccgcga
     4441 tattttttgg tccggtaatc gtcgttctcg tcacggccta tggcggattg ggcgccgcca
     4501 tcgcttgggc acttttgaat tgcggttatc tggctatttc ggcgccgatc atccatgcgc
     4561 gggtgttacg cggagagttg aagacgtggt atctgaccga tgttggcagc ggatttctgg
     4621 cttcgacggc ggtgccttgc atcgtcaagc tcgctctgcc ggccgatata tccccctggt
     4681 tgacgattgt ctgtgtcggc tcgacatggg ccgccagcac gatttgttgc gcgcttgctt
     4741 tgccggaaac ccgtcagttc acggtc
//
LOCUS       DASWPU010000504        14102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_77502,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000504 DASWPU010000000
VERSION     DASWPU010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 14102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14102
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77502"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VGC27_10675"
     CDS             complement(<1..832)
                     /locus_tag="VGC27_10675"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEY0283073.1"
                     /translation="MARQAYRASIFHLLGEPSSEDAAQYFEDGVLVVEDGHVAELGPW
                     DEVAPSLGARVEHFAGGLIVPGFVDSHVHYPQVDIVASRGGDLLEWLSVYTFPAEARF
                     HHKEVAAEAARFFLDQLLRNGTTTALVFATAHKISAEALFEEALARNMRLITGKVLMD
                     RGAPAGISDTAETGYMESRALIRAWHGKGRLGYAVTPRFALTSSERQLELAGRLLSEH
                     PGVRLHTHLSENLEEIAAVRRLFPDCPDYFSVYEKFGLATPHSLFAHCVHLSPSEWTR
                     V"
     gene            complement(833..2155)
                     /gene="deoA"
                     /locus_tag="VGC27_10680"
     CDS             complement(833..2155)
                     /gene="deoA"
                     /locus_tag="VGC27_10680"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEY0283074.1"
                     /translation="MLPQEIIRKKRDGEALSEEEISFLVRGITDNSVSESQISAFAMA
                     VFFRGMTLDERVALTRAMTHSGTVLRWDGLGPVLDKHSTGGVGDKVSLMLAPMVAACG
                     GYVPMISGRGLGHTGGTLDKLGAIPFYRATPDLDTLRRVVREVGCAIIGQTADLAPAD
                     QRFYAVRDVTATVESIPLICASILSKKLAAGLQGLAMDVKCGSGAFMPDLKSAKELAE
                     TIVRISTGAGLPTTALVTDMDEVLGATAGNALEVGEAVDYLTGRFREPRLHGITLALA
                     GEMLALGKLAASPEEGRAKAQAALDSGAAAETFARMVAALGGPKDFLERPEAHLVRAP
                     VTLPFNASRSGFVAGMNARDIGVAVLDLGGGRRRTTDKIDLAVGFSDFRPVGTRVAAG
                     DRIALVHAADETAAKRAVAALGRCVRIEDAVPAPRPLVLERIAAPLGS"
     gene            complement(2161..2571)
                     /gene="cdd"
                     /locus_tag="VGC27_10685"
     CDS             complement(2161..2571)
                     /gene="cdd"
                     /locus_tag="VGC27_10685"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY0283075.1"
                     /translation="MSGPAPEMIELAKLMMARAYAPYSRFAVGAVLRGANGKLYGGCN
                     VENAAYPVGSCAEASAISAMIADGEQRIAEVLVMASGNEMVTPCGACRQRLNEFATDA
                     TLVHLWDPAGSFRKVKLADLLPLSFGPANLGKET"
     gene            complement(2577..4922)
                     /locus_tag="VGC27_10690"
     CDS             complement(2577..4922)
                     /locus_tag="VGC27_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0283076.1"
                     /translation="MTNVAEILETMEYGPAPESAAPAAAWLDKHDRSFDLFIGGEWTP
                     AEAHLDSFNPATGKKLASIAHASEADVDAAVKAARAAQPGWAKIGGAARARYLYAIAR
                     LIQKHSRLFAVLETMDNGKPIRETRDIDVPLAARHFYHHAGWAQLLESEFPGHEALGV
                     AGQIVPWNFPLLILAWKIAPALAAGNAVVLKPSKETSLSALLFAEICAEARLPKGVVN
                     IVTGPGKVGDAIVRHPDVDKIAFTGSTEVGRSIRMALAGTGKKLTLELGGKSPFIVFE
                     DADLDGAVEGTVDAIWFNQGQVCCAGSRLLVQESVAERFLAKLRARMETLRVGDPLDK
                     VTDIGAIVSPTQRAGIDALVKRGVAEGADLFQPKAGCPDGCFYPPTLLTNVEPASTVV
                     QEEIFGPVLTAMSFRTPAEAAQLANNTRYGLAASIWSENINVALDLAPKIKAGVVWVN
                     CTNQFDAACGFGGYRESGFGREGGREGMFAYLKPKAARVHKAPPAPAAEPAIVSFDAI
                     DRTAKLFVGGKQARPDGGTSRAIVSPSGAFVGHAGEGNRKDIRNAVEAAAKAEKWSAT
                     TGHLRAQILYYCAENLSARAAEFAQRLRQMTGAGKAAAAREVESSIRRLFFYAAWADK
                     YDGLVHNPPLRGVALAMNEPIGVIGIACPDQAPLLAFTSLWAPAVTMGNRVVIVPSEK
                     HPLAATDFYQVLETSDMPDGVVNIVTGARDALAQVLAEHRGVEAVWYFGSDRKRIEQA
                     AADDIKRTWTGETIDWIGAGEGRAFLRHATQVKNVWIPYGA"
     gene            complement(4926..5936)
                     /gene="deoC"
                     /locus_tag="VGC27_10695"
     CDS             complement(4926..5936)
                     /gene="deoC"
                     /locus_tag="VGC27_10695"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY0283077.1"
                     /translation="MFKIQPVPSNDHAPESGRNSVAAFDPDLIDSVRVNRSAAERRVA
                     TLPGRRTVKKEWQAAWFVRAVQCIDLTTLSGDDTPGRVRRICAKARQPVRQDLLEAMG
                     LGEAGITTGAVCVYHAMVETAVEALRGTDIPVAAVSTGFPAGLTPLPLRIKEVEASVA
                     AGAREIDIVLNRSNVLTGNYRAVYDEVRAFREACGARGACGEAHLKTIIGAGDLGTLT
                     NVGRASMACMMAGADFIKTSTGKEAVNATLPFALTMVRAIRTWRELTGYKVGFKPAGG
                     ISAAKDVLNYQFVMKEELGNDWLRPNLFRIGASSLLTDIERQLDHFVSGRYSSAHRHP
                     MG"
     gene            6092..6430
                     /locus_tag="VGC27_10700"
     CDS             6092..6430
                     /locus_tag="VGC27_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY0283078.1"
                     /translation="MKLVIAIIQPHKLDSVRTALLNLGVEGMTVTEVHGYGRQKGHKE
                     VYRGAEYTIAFIPKLRVEVAVVDQRVDEVIAAIRQGALSGNIGDGKIFVVELGAAMRV
                     RTGERDGDAL"
     gene            complement(6640..7197)
                     /locus_tag="VGC27_10705"
     CDS             complement(6640..7197)
                     /locus_tag="VGC27_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283079.1"
                     /translation="MSVSGRWSLCAGLLMLMPHAMAEDCFTSTILKPTPFMGNNNEIF
                     KLADGSIWQVKYEYEYLYEYYPNVIVCPNVGRLIIKDKKLNIVKISDSALTASSGRPS
                     RTAQRPAQSNLRLIESQIDGDYNGWTGNTIYKLVNGQVWQQVDAHYHYHYSYSPKVMI
                     YPTNGSFKIHVDGDSDDDVSVQRPN"
     gene            complement(7814..10303)
                     /locus_tag="VGC27_10710"
     CDS             complement(7814..10303)
                     /locus_tag="VGC27_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY0283080.1"
                     /translation="MQSKAWYGVIATLLLLQCGSATAAGLRLSDQDTFETQGLSVIVH
                     QNTFHPVFRDEKIAGIQIILHGDRIATDGEVRLQPTPEQWDPVPMYTERKRGPAPDQL
                     IVSSGYPELPLTYRLEITAEDQGFRVAVHLDRPLPDALVGKAGFNLDFLPTSYFGKSY
                     MIGSTFGMFPRYPNGSMKTGADGLVEPLPLASGNAIVLSPEDPLTRVAITSDGAPLML
                     YDARNKAQNGWFVVRSLLPAGRTTNALVWHVRPHVIPGWIRKPVVSFNQVGYTPERDK
                     TAIIELDPLYRAPKVARLMRLGADGRYQEAYRGEIKPWGKWLRYNYASFDFSRVRQPG
                     IYAIAFGGQTFNPFRIDSNLYSKGVWQPSLDTYLPVQMDHVKVREGYRIWHGASHLDD
                     ARQAPPNHQHFDGYWMGPNTDSPFAAGEHIPGLNVGGWYDAGDFDIRTQTQSQVVRNL
                     VMAREVFHIDWDETTVDEEARSVQIRQPDGIPDDLQQVKHGVLALMAQYKAFGHAIGG
                     IIEPTLQQYTHLGDGASKTDGKIYSAAMGPLQSDGIHSGVPDDRWAFTMHATPLNYDA
                     AAGLAAASRVLRGYDDALAEECLQTALRVWDEEHKRAPVVFENSFNILGRDLDEEEAT
                     LAVELIIATQGRADIKDRLKEMLPYIRKNFARVGWLAARAIPFMDADFKSAIGAALAE
                     AKPQLDAELSKNPFGVPIGMGTWAGSAQVAIFAVQMYLFHEAFPEIVGADYTLRGFDY
                     VLGRHPATNLSFVSSVGTESKLIAYGNNRADFSFIPGGMVPGVLIVRPDFPEMKVAWP
                     FLWYENEYVVDTVSAFILAANAADALTKEKQ"
     gene            complement(10343..10972)
                     /gene="upp"
                     /locus_tag="VGC27_10715"
     CDS             complement(10343..10972)
                     /gene="upp"
                     /locus_tag="VGC27_10715"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY0283081.1"
                     /translation="MQGVTVVNHPLIQHKLTLMRDKNASTKHFRELLNEIGMLICYEI
                     TRDLPIEMVDIETPLTAMKAPALQGKKLVFAPILRAGTAFLDGMLNLVPSARVAFVGL
                     YRDPKTLEAVEYYFKAPEDIAERLVIVMDPMLATGHSAIAAVDRLKARGVSKLRLMCL
                     LAAPEGLEAFTKHHPDVHVWTAAIDSHLNDHGYIVPGLGDAGDRMFGTK"
     gene            11473..12639
                     /locus_tag="VGC27_10720"
     CDS             11473..12639
                     /locus_tag="VGC27_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0283082.1"
                     /translation="MKNSVDRRSFLMATGTAGLMSTLPSAALAQAAAGNVSEVVAQPG
                     FDAAPFAKQSIKFAVIGLDHYHIMGMTAAVQRGGGKLAAFYTKDPKQIADFRAKFGDI
                     KLARSEDEILNDKSIRLVAGAPIPDLRAPLGIRVMRAGKDYLSDKPAITTLEQLADVR
                     KTVKETKRKFAIMYSERLEVRSAIQAGYLVDQGAIGRVIQTINIAPHKVNDVSRPAWF
                     WDKARYGGILTDIGSHQIEQFLYYTKSTTAHVVASQTGNFAHQNRPLFEDFGDMVAVG
                     NGGTGYIRVDWFTPDGLGVWGDGRLFLLGTEGYIELRKYVDVAGRPGGNHLFIVDKNG
                     IRFIDCKDVALPFGPQFVADIIHRTEAAQNQEQALLTAELVLTAQKNATRPIAA"
     gene            12670..14052
                     /locus_tag="VGC27_10725"
     CDS             12670..14052
                     /locus_tag="VGC27_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0283083.1"
                     /translation="MAKGLLTSRRGILKSGIGVAVAASFPAIVPSSVFGATAPSNRLN
                     IGAIGVGRISRIHDMKEIFKHADAQITAVCDLDSKRVELGKQLVNETYAGKFGRDYTG
                     TVGYSDYHELLADKDIDGVLISTPDHQHAILAVHAVRAGKDVYLQKPASLTIAEGRFM
                     SDAVAKTDRILQMGSQQRSISPWPQFHQACELVRNGRIGDLKQVEIGLPGDPSGPEAP
                     PMPVPPNLDYDAWLGSTPEVYYTEIRVHPQDSFDRPGWLRCEQFGAGMITGWGAHHID
                     TAHWGMGAELTGPVEVWGSAEFPASGLWNVHGKFLTHAVYANGVVMDISGDFPNGIKF
                     IGTKGWLFVTRTGEKTTASDPSGTLSQTGITAADPRILTGAIGPNEIHLYVSPEQHRN
                     WLDSMKTRAPNAAPAEIGHRSCSACLLHFIAMHLKRKLYWDPVAERFKNDDEANAMLS
                     RPQRAPYTFG"
BASE COUNT         2606 a         4536 c         4362 g         2598 t
ORIGIN      
        1 cgacccgcgt ccactcgctg ggcgaaagat ggacgcaatg ggcgaacagc gaatgaggcg
       61 tcgccaggcc gaatttctca taaacggaaa aataatcggg acaatcggga aacaagcgcc
      121 gcaccgcggc gatctcttcg agattttcgg agagatgggt atgcaggcgc acgccgggat
      181 gctcgctcag caaccggccg gccagttcga gctggcgctc gctggaagtc agcgcgaaac
      241 gcggcgtcac cgcatagccg agccgtccct tgccgtgcca ggcgcggatc agggcgcggc
      301 tttccatata gccggtctcg gcggtgtccg agattccggc cggcgcgccc cggtccatca
      361 gtactttgcc ggtgatgagg cgcatgttgc gcgccagcgc ttcctcgaac agcgcttcgg
      421 cggaaatctt gtgggcggtg gcgaacacca gcgccgtggt cgtgccgttg cgcagcaact
      481 ggtcgagaaa gaagcgcgcg gcttccgccg ccacctcctt gtggtgaaag cgcgcttcgg
      541 ccggaaaggt gtagaccgac agccattcga gcaaatcgcc gccgcgcgag gcaacgatgt
      601 ccacctgcgg ataatggacg tgagaatcga caaagcccgg cacgatcaat ccgccggcaa
      661 agtgctccac ccgggcgcct aggctcgggg cgacctcgtc ccagggtccg agttcggcca
      721 catggccgtc ttcgaccacc agcacgccgt cttcgaaata ctgcgccgca tcctcggacg
      781 acggctcgcc cagaagatga aaaatcgaag ctctataggc ttggcgcgcc atctatgatc
      841 ccaaaggcgc ggcgatccgc tccagaacga gcggacgggg cgcgggcaca gcatcttcta
      901 tacgaacgca gcgtcctagc gccgccaccg cgcgtttggc ggcggtttcg tccgccgcat
      961 ggacgagcgc gatcctatca cccgcggcga cgcgcgtgcc aaccggacga aaatccgaga
     1021 aaccgaccgc cagatcgatc ttgtctgtcg tcctgcggcg gccgccgccc agatcgagca
     1081 cagccacgcc gatgtcgcgc gcgttcatcc cggcaacgaa gcccgagcgg ctggcattga
     1141 acggcagtgt cacgggcgcg cggaccagat gggcttccgg cctttcgaga aaatctttgg
     1201 ggccgcccag cgccgcgacc atcctggcga aagtctccgc cgccgccccc gaatcgagtg
     1261 cagcctgcgc cttggcccgg ccttcttccg gtgacgcggc cagcttgccg agcgccagca
     1321 tctcgcccgc cagcgccaat gtgatcccat gcaggcgggg ctcgcggaag cggccggtga
     1381 ggtaatcgac ggcttcgccc acttccagcg cattaccggc ggtggcgccg agcacttcgt
     1441 ccatatccgt gaccagagcg gtcgtgggca agcccgcgcc agtggagatt ctcacaatag
     1501 tttccgcaag ttccttcgcg ctcttcaaat ccggcatgaa ggcgccggag ccgcatttca
     1561 cgtccatcgc caagccttgc agtccggccg ccagcttctt cgacaggatc gaggcgcaaa
     1621 tcagcggaat cgattcgacc gtcgcggtga cgtcgcgcac ggcatagaag cgctggtcgg
     1681 ccggggccag gtcggcggtc tggccgatga tggcgcagcc cacctcgcgc accacccggc
     1741 gcagcgtgtc gagatcgggc gtggcgcgat agaaggggat ggcgccaagc ttgtcgagcg
     1801 ttccgccggt gtgcccaagt ccgcgccccg agatcatcgg cacgtagccg ccgcaagccg
     1861 ccaccatcgg cgccagcatc aaactcacct tgtcgcccac cccgccggtg gaatgcttgt
     1921 cgagcaccgg gccgagtccg tcccaccgca gcacggtgcc ggaatgcgtc atggcgcggg
     1981 tcagcgccac gcgctcgtcc agcgtcatgc cgcgaaagaa caccgccatg gcgaaagccg
     2041 agatctggct ctcgctgacg ctgttgtcgg tgatgccgcg caccaggaag gagatttcct
     2101 cctcgctcag cgcctcgccg tcgcgcttct tgcggatgat ttcctgcggg agcatgagcc
     2161 ttatgtttcc ttgcccaaat tcgccgggcc gaaggaaagc ggcaagaggt cggcgagttt
     2221 caccttgcgg aagctgccgg cagggtccca gagatggacc agcgtcgcgt cggtcgcaaa
     2281 ctcgttcagg cgctggcggc aggcgccgca cggcgtcacc atttcgttgc cggacgccat
     2341 caccagcact tcggcgatgc gctgctcgcc gtccgcgatc atcgccgaga tcgccgaagc
     2401 ctcggcgcaa ctgcccaccg gataggccgc gttctcgaca ttgcagcccc cgtatagctt
     2461 gccgttcgcg ccgcgcagca ccgcgccgac cgcgaagcgg gaatagggcg cgtaggcgcg
     2521 cgccatcatc agcttcgcca attcgatcat ttcgggcgcg gggccgctca tatcaattac
     2581 gctccgtagg gaatccagac gttcttgacc tgcgtcgcgt ggcgcaggaa ggcgcgtccc
     2641 tcgcccgccc cgatccaatc gatggtttcg ccggtccagg tgcgcttgat gtcgtcggcg
     2701 gcggcctgtt cgatgcgctt gcggtcggaa ccgaaatacc agaccgcttc cacgccgcga
     2761 tgttcggcca agacctgcgc cagcgcatcg cgcgcgccgg tgacgatgtt gaccacgccg
     2821 tccggcatgt cggaggtttc gagcacctga tagaaatcgg tcgccgccag cggatgcttt
     2881 tccgacggca cgatgacgac gcgattgccc atcgtcacag ccggcgccca cagcgaggtg
     2941 aacgccagca gcggcgcttg gtccgggcag gcgatgccga tgacgccgat cggttcgttc
     3001 atcgccagcg ccaccccgcg cagcggcgga ttgtggacca gcccgtcata tttgtcggcc
     3061 caagcggcat agaagaaaag ccgccggatg gagctttcca cttcgcgcgc ggcggcggcc
     3121 ttgcccgcgc cggtcatctg ccggaggcgc tgggcgaact cggcggcgcg ggccgaaaga
     3181 ttctcggcgc agtaataaag aatctgcgcg cggagatgac cggtcgtcgc ggaccatttt
     3241 tccgccttcg ccgccgcttc caccgcgttg cggatgtcct tgcggttgcc ctcgccggca
     3301 tggccgacga aggcgcccga aggcgagacg atggcgcggc tggtgccgcc gtcgggccgc
     3361 gcctgcttgc cgccgacgaa cagcttcgcc gtgcggtcga tggcatcgaa agaaacaatc
     3421 gcgggttccg ccgcgggcgc gggcggtgct ttgtgcaccc ttgccgcttt cggtttcaga
     3481 taggcgaaca tgccttcgcg gccgccctcg cggccgaagc cggattcgcg atagccgccg
     3541 aagccgcaag cggcgtcgaa ctggttggtg cagttcaccc acaccacgcc cgccttgatc
     3601 ttgggcgcca gatcgagcgc gacgttgatg ttctcgctcc agatgctggc ggcgaggccg
     3661 tagcgcgtgt tgttggcaag ctgcgccgct tcggcgggcg tgcggaaact catcgccgtc
     3721 agaaccgggc cgaagatttc ctcctgcacc accgtggacg ccggctcgac attggtcagc
     3781 agtgtcggcg gatagaaaca gccgtccggg cagcctgctt tcggctggaa caggtccgcg
     3841 ccttccgcca cgccgcgctt caccagcgca tcgatgccgg cgcgctgcgt cggcgagacg
     3901 atggcgccga tatccgtcac cttgtcgagc ggatcgccga cccgcagcgt ttccatccgt
     3961 gcccgcagct tggcgagaaa gcgctcggcg acgctttcct gcaccaagag gcgcgagccc
     4021 gcgcagcaga cctggccctg gttgaaccag atggcgtcca ccgtgccttc caccgcgcca
     4081 tcgagatcgg cgtcctcgaa cacgataaac ggcgatttgc cgccgagttc gagggtcagc
     4141 ttcttgccgg tgcccgccag cgccatgcgg atggagcggc ccacttccgt cgagccggta
     4201 aacgcgatct tgtccacgtc gggatggcga acgatggcgt cgcccacttt tcccggcccg
     4261 gtgacgatat tgacgacgcc cttgggcagg cgcgcctcgg cgcagatttc ggcgaacagc
     4321 agcgccgaca ggctggtttc cttcgacggt ttcagcacca cggcattgcc cgccgccagc
     4381 gcgggcgcga tcttccacgc caggatcagc agcgggaaat tccacggcac gatctgtccg
     4441 gcgacgccga gcgcttcgtg gccggggaac tcgctttcga gaagctgcgc ccagccggca
     4501 tgatgataga agtggcgcgc ggcaagaggc acgtcgatgt cgcgcgtctc gcggatcggc
     4561 ttgccgttgt ccatcgtctc cagcaccgcg aacagccgcg aatgtttctg gatcagccgg
     4621 gcgatggcat agagatagcg ggcgcgcgcc gcgccgccga tcttggccca gcccggctgc
     4681 gccgcccgcg ccgccttcac cgccgcatcg acatccgctt cagaggcgtg cgcgatggaa
     4741 gccagcttct tgccggtcgc cggattgaaa ctgtcgagat gcgcctccgc cggggtccat
     4801 tcgccgccga tgaagaggtc gaagctgcga tcatgcttgt cgagccaggc tgccgccggc
     4861 gcggcgcttt ccggcgccgg accatattcc atggtctcca atatttcagc gacgtttgtc
     4921 atgttctagc ccatggggtg gcggtgcgcc gaggaatagc gcccgctgac gaaatgatcc
     4981 aattgccgct cgatgtcggt gagcaggctg gaagcgccga tgcggaacag gttcggccgc
     5041 agccagtcgt tgccgagttc ctctttcatc acgaactggt agttcagcac atccttggcg
     5101 gcggaaatgc cgcccgccgg cttgaagccc accttatatc cggtcagctc gcgccaggtg
     5161 cggatcgcgc gcaccatcgt cagcgcgaaa ggcagcgtcg cattgacggc ttccttgccg
     5221 gtggaggtct tgatgaaatc cgcgcccgcc atcatgcagg ccatcgaggc gcgcccgaca
     5281 ttggtcagcg tgccaaggtc tcccgccccg atgatcgtct tcagatgcgc ctcgccgcag
     5341 gccccgcggg ccccgcaagc ttcacggaag gcgcgcactt catcatagac ggcgcggtaa
     5401 ttgccggtga gaacgttgga gcggttgagc acgatatcga tctcgcgcgc gcccgcggcg
     5461 accgaagctt ccacttcctt gatgcgcagc ggcagcggcg tcagcccggc ggggaaaccc
     5521 gtcgataccg ccgccaccgg aatatccgtg ccccgaagcg cctcgaccgc cgtttccacc
     5581 atcgcgtgat agacgcaaac cgcgccggtc gtgatccccg cttcgccgag gcccatagct
     5641 tcaagcagat cctgccgcac cggctggcgc gccttggcgc agatgcggcg gacgcggccg
     5701 ggcgtgtcgt cgcccgacag cgtggtcagg tcgatgcact gcacggccct gacgaaccat
     5761 gccgcctgcc actctttctt gacggtgcga cggccgggca gcgtggcgac gcgccgctcg
     5821 gcggcgctgc ggttgacgcg gacggagtcg atcagatcgg ggtcaaaggc ggcgacggag
     5881 ttgcgcccgc tctcgggcgc gtgatcgttg gaagggaccg gctgaatctt gaacattttt
     5941 ccaagctttc accggggaaa gggtgagtca agcgaaccat gagccgcagc ctgtgcacga
     6001 tttccatcgc acaatgctgc gcactatcac tcgatttcgc atcgcacggt gaaaccggct
     6061 ataacatcaa cacataacag gggggcacgc catgaaactg gtcatcgcca tcattcaacc
     6121 gcacaagctc gattccgttc gcaccgcgct tctcaacctt ggagtggaag gcatgacggt
     6181 gacggaagtg cacggctacg gccgccagaa aggccacaag gaagtctatc gcggcgcgga
     6241 atataccatc gccttcattc ccaagctcag ggtcgaggtc gccgtggtgg accagcgcgt
     6301 ggacgaagta attgccgcca tccgccaagg cgcgctaagc ggcaatatcg gcgacggcaa
     6361 aatcttcgtc gtcgaacttg gcgccgccat gcgcgtgcgc accggtgagc gggatgggga
     6421 cgcgctgtag tgctgtaaga gattgtatcg acgtttcccc taatgcgtca tgaccgccct
     6481 tggttgctag cgaaagcgta gcgacgccat tccgcctgtc gcgccgaagc gcttacgcaa
     6541 aggcggattg ccgcgcgcta cgaggccttt gatgcaattc ttactctgac ccaatcttat
     6601 gcaatccaac caatcttact cgcagacata tctgacctgt taattgggcc gttgcactga
     6661 cacatcgtcg tcgctgtcgc cgtcgacatg aattttgaag cttccattcg ttgggtagat
     6721 cataactttt ggcgaatatg agtagtggta atggtaatgt gcatctacct gttgccagac
     6781 ttggccgttc accaatttgt agatcgtgtt gcctgtccag ccgttgtaat cgccgtcgat
     6841 ttggctttcg atcaggcgca aattgctttg tgcaggccgt tgtgcggttc ggctgggccg
     6901 cccagatgat gccgttagcg cgctgtcaga tatctttacg atattaagtt tcttatcctt
     6961 gattatcagt cttccgacgt tagggcacac gatgacgttg gggtagtact cgtaaagata
     7021 ctcgtattca tatttgacct gccaaatcga accatcggcc agtttgaata tctcgttgtt
     7081 gttccccata aacggcgtcg gcttaaggat cgtggacgtg aaacaatcct ccgccatcgc
     7141 gtggggcata agcataagaa gcccggcgca cagtgaccat cttcctgaaa cactcatctg
     7201 agcgtctctt ccccgccggt gcgcagtata tagggattct catgtcccat ctcactgatt
     7261 ttctctgtgt ttatcatgct ctaagaaagg tggtcggact ggttttatcc ggcgccacag
     7321 caaaatcgtg gcacgattga ccatcgttca ccccgccacc acgcgtccgg ctggcacaac
     7381 gcagcgcaga atatcccgcc ttatttcatt ccgtatacgg atgatattgc atctcatctg
     7441 cgaacattaa atctcgcagc gggggcttga acgcggattt tcgttcccca tatgtccaag
     7501 tcgagttcca aaggaaacgc catgctcgtc tgtgtatcgc ggggccggct caaagccccg
     7561 atccgaagct gacgcgagac catacagggc ttggatgcga gctcgcgcgc atccgcaaag
     7621 agcaagccgg cgatgtgccg cccaggcggc cgatagcaga acgctcgctc cgcgaagctc
     7681 gaggcaaagc aaaggtgctt gcggttccgc gcggcggttc cgcttgccgg ccgtagctta
     7741 ccaaaggcgg gttcgccggg cgcacgtcaa cgaaacgact tcgcgttcgc gcagcccgcc
     7801 ttttacttga ggtctactgc ttctctttcg tcagggcgtc cgccgcgttc gccgccagga
     7861 tgaatgcgga aacggtatcg acgacatatt cgttttcgta ccacaggaac ggccacgcca
     7921 ccttcatctc cggaaaatcc ggccgcacga tcagaacgcc cggaaccatg ccgccgggaa
     7981 tgaaggagaa atccgcccgg ttgttgccat aggcgatgag cttcgactcc gtccccaccg
     8041 aagagacaaa ggacaggttg gttgcaggat gccggcccag aacatagtcg aaaccgcgca
     8101 gcgtgtagtc cgctccgacg atctcgggaa acgcctcgtg gaagaggtac atctggacgg
     8161 caaatatcgc cacttgggcg gagccggccc aggtgcccat gccgatcggt acgccgaagg
     8221 ggttcttcga cagttcggcg tccagttggg gcttggcttc cgccaacgcc gcgccgatgg
     8281 ccgacttgaa atcggcatcc atgaagggaa tggcgcgcgc ggcgagccat cccactctgg
     8341 cgaagttctt tcggatatac ggcaacattt ccttcaggcg gtctttaata tcggcccggc
     8401 cttgcgtggc gatgatcagt tcgacggcca gcgtcgcttc ttcctcgtcc aagtctcttc
     8461 ccaggatgtt gaaggaattc tcgaacacca ccggcgccct tttgtgctcc tcgtcccaga
     8521 cgcgcagcgc cgtctgcagg cactcctccg ccagcgcgtc gtcatagccg cgcaacacgc
     8581 ggctggcggc ggcgaggccc gccgcggcat cgtagttgag cggcgtggcg tgcatcgtga
     8641 aggcccagcg gtcgtccggc acgcccgaat ggatgccgtc gctttgcagc ggccccatcg
     8701 cggcggaata gattttaccg tccgtcttcg aggcgccgtc gcccagatgc gtgtattgct
     8761 ggagcgtcgg ctcgatgatc cccccgatgg cgtggccgaa ggctttgtac tgcgccatca
     8821 gcgccagcac gccgtgcttg acctgttgca gatcgtcggg aatgccgtcg ggctggcgga
     8881 tttgaaccga ccgcgcctcc tcgtccaccg tggtttcgtc ccagtcgata tggaagactt
     8941 ccctggccat gacgagattt cgaaccacct gcgactgagt ctgggtgcgg atgtcgaagt
     9001 cgccggcgtc ataccagcct ccgacgttga gacccggaat atgctccccc gcggcaaagg
     9061 gcgaatccgt gttcggcccc atccagtagc cgtcgaaatg ctgatggttc ggcggcgcct
     9121 gccgcgcgtc gtccagatgc gacgcgccgt gccagatgcg atagccctcg cgcactttca
     9181 catggtccat ctgtacggga agataggtat ccagcgaggg ctgccagacc cccttgctgt
     9241 aaaggttgct gtcgatccgg aagggattga aggtctgtcc tccgaaggcg atggcataga
     9301 tgcccggctg gcggacgcgc gaaaaatcaa aggatgcata gttataccgc agccatttgc
     9361 cccagggttt gatctcgccg cgataggctt cctgataccg gccgtccgcg ccgagccgca
     9421 tcaggcgcgc aaccttcggc gcgcggtaca gcggatcgag ttcgatgatg gccgtcttgt
     9481 cgcgttcggg cgtgtagccc acctggttga aggagaccac cggcttgcgg atccagcccg
     9541 gaatcacatg cggacggacg tgccacacca aggcgttggt agtgcggccg gccggaagca
     9601 gcgagcgcac cacgaaccag ccgttctgcg ccttgttgcg ggcgtcgtag agcatcagcg
     9661 gcgcgccgtc ggaggtgatc gccacccgcg tcagagggtc ttccggcgac aggacgatgg
     9721 cgtttccgct ggccagcggc agtggctcga ctaagccgtc cgcgccggtt ttcatcgaac
     9781 cgttcggata gcgcggaaac atgccaaacg tgctgccaat catatagctc ttgccgaaat
     9841 agctggtggg cagaaaatcg agattgaatc ccgccttccc caccagcgca tcgggcagcg
     9901 gccggtcgag atgaaccgcg acacggaagc cttggtcttc cgccgtaatt tcgaggcgat
     9961 acgtcagcgg cagttcgggg taccctgaag atacgatcaa ttgatcggga gccgggccgc
    10021 gcttgcgttc cgtgtacatc ggaaccgggt cccattgttc gggggtgggc tgcaagcgca
    10081 cttcgccgtc ggtcgcgatc cggtcgccgt gcaggatgat ctgtatcccg gcgatctttt
    10141 cgtcgcgaaa gaccggatga aacgtattct gatggacgat gacgctcagt ccctgcgtct
    10201 cgaaagtgtc ctggtcgctc agcctgagac cggcggccgt cgcgctgccg cactgtagca
    10261 aaagaagcgt tgcgatgact ccataccaag ctttgctttg cacgattacc tccaggaaat
    10321 aacgaagctg gaaacggctt tcctacttgg ttccgaacat gcggtcgccg gcgtcgccca
    10381 ggccgggcac gatatagccg tgatcgttga gatggctgtc gatcgccgcc gtccagacgt
    10441 ggacgtcggg atggtgcttg gtgaaggcct ccagcccttc gggcgcggcc agcaggcaca
    10501 tcaggcgcag cttgctgacg ccgcgcgcct tcaacctgtc caccgccgcg atggcggagt
    10561 ggccggtcgc cagcatgggg tccatcacga tcaccagacg ctcggcgata tcctccggcg
    10621 ccttgaaata atattcgacg gcttccagtg ttttgggatc gcggtaaagc ccgacgaagg
    10681 cgacgcgcgc cgacggcacc aggttcagca tcccgtccag gaacgccgtc cccgctctca
    10741 ggatcggcgc gaacaccagc ttcttgcctt gaagcgccgg ggctttcatg gccgtcagcg
    10801 gggtttcgat gtccaccatc tcgatgggca gatcgcgcgt gatctcgtag cagatcagca
    10861 tgccgatctc gttgagcagc tcgcggaaat gcttggtgct ggcgttcttg tcccgcatca
    10921 acgtcagctt gtgctggatc agcggatgat taacgacggt gacgccctgc atcgtcttct
    10981 ctcccagttt gcggtgagta agaagaaagc gcgcgcccgg cgcaaggcgc accttcgggc
    11041 ggcgtcgaac ggccgcacag ctacttgtgg ataagaggat tttcgcacgc ggtgcgtcag
    11101 atttcggctt ccagccggtc gtcgccgcgc atgcggtaga gcgcgctgag ccgatccagc
    11161 agacgacgaa aatcgcgacg atcagctagc ccagcgtgcc gatgccgtcg tcgaagatgc
    11221 cgcggagcgc gcagcccata aaccatcctt ccggatataa gtatgatgta tagccgaaac
    11281 cctcgtgctg gcaacgccgc gatccttcct tggacgactg ccggttgcgg aaattgcctt
    11341 ttgcatccga gccgcgcctc ctggcgttgt gatgttaaat cgcctcatca ctttcgccgg
    11401 cgacggtgct attctgtcaa tgggagcggt accaaaacgg ccggacgggt cgttggacag
    11461 gaaggaataa cgatgaagaa ttcggtggac cggcgttcgt ttctgatggc gaccggcacg
    11521 gccggtttga tgtcgacgct gccgtcggcg gcgttggctc aggccgcggc tggaaacgtc
    11581 tcggaggtcg tggcgcaacc cggcttcgat gcggcgccgt ttgcgaagca atcgatcaaa
    11641 ttcgcggtga taggcctcga tcattaccac atcatgggca tgacggccgc ggtccagcgc
    11701 ggtggcggca agctcgccgc cttctacacc aaagacccga agcaaatcgc tgatttccgc
    11761 gcgaagttcg gcgacatcaa gctggcgcgc agcgaagacg agattttgaa cgacaagtcg
    11821 atccgcttgg tcgccggcgc gccgatccct gacctgcgcg cgccgctcgg catccgcgtc
    11881 atgcgcgcag gcaaggacta tctcagcgat aagcccgcca tcaccacgct cgaacagctt
    11941 gccgatgtcc gcaagaccgt caaggagacg aagcgcaaat tcgccatcat gtattcggag
    12001 cgcctcgaag tccgctcggc gatccaggcg ggctatctgg tcgatcaggg cgccatcgga
    12061 cgcgtcatcc agaccatcaa catcgccccg cataaggtca acgacgtgtc gcggccggct
    12121 tggttctggg acaaggcgcg ctatggcggc atcctcaccg atatcggctc gcaccagatc
    12181 gagcagtttc tctattacac taaatcgacg acggcacatg tcgtcgcctc gcagaccggc
    12241 aacttcgcgc atcagaatcg tccgctgttc gaggactttg gcgacatggt cgcggtcggc
    12301 aacggcggca ccggttacat ccgcgtcgac tggtttacgc cggacggcct cggcgtgtgg
    12361 ggcgacggaa gactcttcct gctcggcacc gaaggctata tcgaacttcg caaatacgtc
    12421 gatgtcgcgg ggcggcccgg cggcaaccac ctcttcatcg tcgacaagaa tggcatccgc
    12481 ttcatcgact gtaaggacgt cgcgctgccc ttcgggccgc agtttgtggc cgacatcatc
    12541 catcgcaccg aggcggctca gaaccaggaa caggcgctgc tgacggcgga actcgtgctc
    12601 acggcgcaga agaacgcgac gcggccgatc gcggcctgac gcacccgatc aagaaccagg
    12661 aaccggaaaa tggcaaaagg gcttctgaca tcgcgtcgcg gaatcttgaa gtcgggcatc
    12721 ggcgtcgccg ttgccgcttc ctttccggcc atcgtgccgt cgagcgtgtt cggcgccacg
    12781 gcgccgagca acaggctcaa tatcggcgcg atcggcgtgg ggcggatttc gcgcattcac
    12841 gacatgaaag agattttcaa gcacgccgac gcacagatca ccgcggtctg tgatctcgac
    12901 tccaaacggg tcgaattggg caagcagctt gtcaacgaaa cctatgccgg gaaattcggg
    12961 cgggactaca ccggcaccgt cggctacagc gactatcacg agctgctggc cgacaaggac
    13021 atcgacggcg tcctgatctc gacgcccgat catcagcacg cgatcctcgc cgtccacgcg
    13081 gtccgcgccg gcaaggacgt ctatctgcaa aagcccgcct cgctgaccat cgccgaaggc
    13141 cgcttcatga gcgacgcggt cgccaagacc gaccgcatcc tgcagatggg ttcgcagcag
    13201 cgctccatca gtccgtggcc gcagttccat caggcttgcg agctggtgcg caacggccgc
    13261 atcggcgatc tcaagcaggt cgagatcggc ctgcccggtg atccgtccgg ccccgaggcg
    13321 ccgccgatgc cggtgccgcc caacctcgat tacgacgcct ggctcggttc gacgcccgag
    13381 gtctactaca ctgaaatccg cgttcacccg caggattcgt tcgaccgtcc cggctggctg
    13441 cgttgcgaac agttcggtgc cggcatgatc accggctggg gcgcgcatca tatcgacacg
    13501 gcgcactggg gcatgggcgc ggaactcacc ggcccggtcg aagtctgggg cagcgcggaa
    13561 tttcccgcga gcgggctatg gaacgtccac ggcaagttcc tgacccatgc ggtttacgcc
    13621 aacggcgtag tgatggacat ctcgggcgac ttcccgaacg gcatcaaatt catcggcacc
    13681 aagggctggc tcttcgtcac ccgtaccggt gaaaaaacga cggcgagcga tccctccggc
    13741 acgctgagcc agaccggcat cacggcagcc gatcccagga tcctcaccgg cgcgatcgga
    13801 ccgaatgaaa tccatctcta tgtgagcccc gaacaacacc ggaactggct ggactcgatg
    13861 aagacccgcg cgccgaacgc ggcacccgcg gaaatcggtc accgctcctg ctcggcctgc
    13921 ctgctgcact tcatcgcgat gcacctcaag cgcaagctgt actgggaccc ggtggccgag
    13981 cgcttcaaga acgacgacga agcgaatgcg atgttaagcc gcccgcagcg cgcgccttac
    14041 acgttcggct gagggatggg cctgatgctg cgttctcgct gcggcggatc aggcccggat
    14101 tg
//
LOCUS       DASWPU010000505         3876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_9902, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000505 DASWPU010000000
VERSION     DASWPU010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9902"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /gene="proB"
                     /locus_tag="VGC27_10730"
     CDS             complement(<1..1031)
                     /gene="proB"
                     /locus_tag="VGC27_10730"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY0283084.1"
                     /translation="MTDILATAHTVVVKVGSALLVDARTGSLRRDWLKSLSADIAKLK
                     SEGKDVLLVSSGAIALGRRALKLRSGVLKLEESQAAAAAGQVRLAAAYADILARDGIV
                     AAQVLLTLDDTEERRRYLNARATLKTLLELGAVPVINENDTVATAEIRFGDNDRLAAR
                     VASMMEADRLILLSDVDGLYSADPTLDAEARHIGEVRTITPQIKAMAHGSASAVGSGG
                     MTSKLVAAKIATAAGCEVVIAKGKTESPIAALRAGARATRFRASTTPAAARKRWIAGV
                     LKPQGTLIIDAGAAKALAAGKSLLPAGIRQVDGRFERGDAVLVRDGEGREIARGLAAY
                     GASDAERIAG"
     gene            complement(1093..2130)
                     /gene="obgE"
                     /locus_tag="VGC27_10735"
     CDS             complement(1093..2130)
                     /gene="obgE"
                     /locus_tag="VGC27_10735"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY0283085.1"
                     /translation="MKFLDTAKVYIQSGKGGNGCIAFRREKFIEYGGPWGGDGGKGGD
                     VWAEAVENLNTLIDYRFQQHVRAKTGEGGKGKDMHGAGGGDAVMRVPVGTQIYEEDGE
                     TLIADLASAGDRVMLLRGGNGGFGNAHFKSATNQAPRYANPGQPGEEKAIILQLKLIA
                     DVGLIGLPNAGKSTFLARVSQARPKIADYPFTTLHPQLGVVRIDATDFVLADLPGLIE
                     GAHEGVGLGDKFLGHAERCGAILHLIDGTESEVALAYKTIRAELEAYGQGLTGKPEIV
                     ALNKADAIPKAELAKKRAALKKACGHDVFVISGVSGEGVEDVLRALAKEIAKRRAKEK
                     AEGARTKVWAP"
     gene            complement(2376..2918)
                     /locus_tag="VGC27_10740"
     CDS             complement(2376..2918)
                     /locus_tag="VGC27_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0283086.1"
                     /translation="MPRLETDRLLLRPPEFRDVPAITTWAGDWDVAKNLACVPYPYRE
                     EDAREFVARAVEKLATGEGYCFSIVRKRDGVFMGACGLQLKDGRFELGYWLGKPFWRQ
                     GYATEAAKRVVSFAFRDLKATSVWAGWFHDNPASGRVLEKLGCRPDGAAPRASLARGQ
                     EVTCHLMALTREEFGRKRAA"
     gene            complement(3022..3303)
                     /gene="rpmA"
                     /locus_tag="VGC27_10745"
     CDS             complement(3022..3303)
                     /gene="rpmA"
                     /locus_tag="VGC27_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020056123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY0283087.1"
                     /translation="MAHKKAGGSSRNGRDSAGQRLGVKLFGGQKIAAGNVIVRQRGTQ
                     FHPGENVGMGKDHTLFALTDGQVAFRKGFKRRTFVAVVAAAPATKSAAK"
     gene            complement(3315..3623)
                     /gene="rplU"
                     /locus_tag="VGC27_10750"
     CDS             complement(3315..3623)
                     /gene="rplU"
                     /locus_tag="VGC27_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY0283088.1"
                     /translation="MFAVVRTGGKQYKVASGDVLTIEKVAGEVGGELKLGEVLMLGGE
                     AAKTGAPLVAGASVTAEIVAQGKGEKVVAFKKKRRKNTHRKRGHRQPFTKIKITGIEA
                     "
BASE COUNT          604 a         1324 c         1218 g          730 t
ORIGIN      
        1 cccgcgatgc gttcggcgtc ggaggcgccg taagcggcca gaccgcgcgc gatctcgcgg
       61 ccttcgccgt cgcgcaccag aaccgcgtcg ccgcgttcga agcggccgtc cacctgacgg
      121 atgccggccg gcaacagact cttgcctgcg gccagcgcct ttgccgcgcc ggcgtcgatg
      181 atcagcgtgc cttgcggctt gagcacgccg gcgatccagc gcttgcgcgc ggcggccggc
      241 gtggtcgagg cgcgaaagcg ggtggcgcgg gcgccggcgc gcagcgccgc gatggggctc
      301 tccgtcttgc ccttggcgat gacgacttcg cagcccgccg cggtggcgat cttcgccgcg
      361 accagtttcg acgtcatgcc gccgctcccg accgccgacg ccgaaccgtg cgccatggcc
      421 ttgatctgcg gcgtgatcgt tctgacttcg ccgatatggc gcgcctccgc gtcgagggtg
      481 ggatcggcgc tgtatagtcc gtccacgtcg gaaagcagta tcagccggtc ggcttccatc
      541 atcgaggcga cgcgggccgc cagccggtcg ttgtcgccga agcggatttc ggcggtggcc
      601 acggtgtcgt tttcgttgat caccggcacg gcgcccaatt cgagcaaagt cttcagcgtg
      661 gcgcgcgcgt tgagatagcg gcggcgctcc tcggtgtcgt cgagggtgag cagaacctgt
      721 gccgcgacga tgccgtcgcg cgccaggatg tcggcatagg ccgcggccag gcgcacctgc
      781 cccgccgccg ccgccgcctg gctttcctcg agctttagca ctccgcttct cagcttcagc
      841 gcccgccgac cgagcgcgat ggcgccggag gataccagca gcacgtcctt gccttcgctt
      901 ttcagcttgg cgatgtcggc gctcagcgat ttcagccagt cgcggcgcag gctgccggtc
      961 ctggcatcga ccaacagcgc ggagccgacc ttgacgacga cggtatgcgc ggtggcgaga
     1021 atgtcggtca tggagacgtg cctttaccct ccctcaaggg aagggcggaa atacttttga
     1081 agagcgaatt cgtcatggcg cccagacctt tgtccgtgcg ccttccgctt tctccttggc
     1141 gcggcgtttg gcgatttcct tcgccagcgc gcgcagcacg tcttccacgc cctcgcccga
     1201 aacacccgag atcacaaaaa catcgtgacc acaggctttt ttaagtgcgg cgcgcttttt
     1261 cgccaattcc gctttgggaa tcgcgtccgc cttgttgagg gcgacgatct cgggcttgcc
     1321 ggtcaagcct tgtccataag cctcaagctc ggcgcggatg gtcttgtaag cgagagcgac
     1381 ctcgctttcg gtgccgtcaa tcagatgcag aatggcgccg cagcgttccg catgaccgag
     1441 gaacttgtcg cccaggccga cgccttcgtg cgcgccttcg atcaggccgg gcagatcggc
     1501 cagaacgaaa tccgtcgcgt cgatgcgcac caccccgagc tggggatgca gcgtggtgaa
     1561 aggatagtcg gcgatcttcg gtcgcgcctg cgagacgcgc gccaggaaag tggacttgcc
     1621 cgcgttgggc aggccgatca gccccacgtc ggcgatcaac ttcaattgaa ggatgatcgc
     1681 tttctcttcg cccggctggc cgggattggc gtagcgcggc gcctgattgg tggcgctctt
     1741 gaaatgggcg ttgccgaagc cgccattgcc gccgcgcaag agcattacgc ggtcgcccgc
     1801 gctcgccaga tcggcgatca gggtttcgcc atcctcttcg tagatttgcg tgccgacggg
     1861 gacgcgcatc accgcgtcgc cgccgcccgc gccgtgcatg tccttgccct tgccgccttc
     1921 gccggtcttg gcgcggacat gctgctggaa gcggtaatca atcagcgtgt tgaggttctc
     1981 caccgcctcc gcccagacgt cgccgccctt gccgccgtcg ccgccccaag gcccgccata
     2041 ttcgatgaac ttctcgcgcc ggaacgcgat gcagccgttc ccgcccttgc cggactggat
     2101 atagaccttg gccgtatcga ggaacttcat cgcttcgcct ttggactctc atgcttcgac
     2161 aggctcagca tgaggggttg gtatccgtca ccctgagctt gcccgccttc gccatgctac
     2221 ggcggaccta agttcgatgc cctgcccgcc gaagccttgg cgtaggcggg acccggccac
     2281 ccagcagccg cgcgtccgcg cggcgaaaga tactttcttc acctggatgg cccgcttttg
     2341 cgggccatga caataaaggg gggagccgca taacatcatg ccgctctttt tcgcccgaat
     2401 tcctcgcgcg tcaacgccat caagtggcag gtgacctcct gaccgcgggc aaggctggcg
     2461 cgcggcgccg ccccgtccgg gcggcagccc agcttttcca gcacccgccc ggaggccgga
     2521 ttgtcgtgaa accagcccgc ccagacgcta gtggccttga ggtcgcggaa ggcgaacgac
     2581 accaccctct tcgccgcctc ggtagcatat ccctggcgcc agaacggctt gcccagccaa
     2641 tagccgagct cgaagcggcc gtccttgagc tgtagcccgc aggcgcccat gaacacgccg
     2701 tcgcgcttgc ggacgatgga gaagcaatag ccctcgccgg tggcgagctt ctccacggcg
     2761 cgggcaacga attcgcgcgc atcctcttcg cgatacggat aggggacgca ggcaaggttc
     2821 ttcgcgacgt cccaatcgcc cgcccaagtc gtgatcgccg gcacgtcgcg aaactccggc
     2881 gggcgtaaga gcagtctgtc cgtttccaat ctgggcatgt cattccctcc gctctgcgag
     2941 ggaacaaaaa agggagatgg cggaccatct ccctcgcctc catgggccgc cgaattcgtg
     3001 gcggcctatg tcttcagccg cctattttgc cgcgctcttc gtcgccggag ccgccgctac
     3061 gaccgccacg aaggtgcggc gcttgaagcc cttgcggaac gcgacttggc cgtcggtaag
     3121 ggcgaacagc gtatggtcct tgcccatgcc gacattctcg ccgggatgga attgggtgcc
     3181 gcgctggcgc acgatcacgt tgcccgctgc gatcttctgg ccgccgaaca gtttgacgcc
     3241 gagcctctgg cccgccgagt cgcgaccgtt gcgcgaagaa ccgcctgctt tcttgtgagc
     3301 catgatctgg ttccttaggc ctcgatgccg gtgatcttga tcttggtgaa gggctgacga
     3361 tggccgcgct tgcgatgggt gttcttgcgg cgcttcttct tgaaggccac caccttctcg
     3421 cccttgccct gggccacgat ttcggcggtc acggaagcac cggccaccag aggcgcgccg
     3481 gtcttggcag cctcgccgcc gagcatcagg acttcgccga gtttcagctc gccgccgacc
     3541 tcgcctgcga ccttttcgat ggtcaggacg tcgcccgatg ccaccttgta ctgcttgccg
     3601 cccgtgcgga ccaccgcgaa catgagctaa atccttgaaa agaaaagaat tccctgaaag
     3661 cgtctgccct cggggagcgc ggcaatatac tgacgcaaag ggcggggtca agcgggcgtt
     3721 tctacttgcc ctctccttcc tctttccgct tcacgaacag ccccaccacg gccgttgccg
     3781 ccagcgccag gccgacggcg atgccggcga acttcgcggc ggtcgcttcc gaggccagca
     3841 gcgccagagt cgggctgggg ccaacggtca gcatga
//
LOCUS       DASWPU010000506         5091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_89931,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000506 DASWPU010000000
VERSION     DASWPU010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89931"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2274)
                     /gene="putA"
                     /locus_tag="VGC27_10755"
     CDS             complement(<1..2274)
                     /gene="putA"
                     /locus_tag="VGC27_10755"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEY0283089.1"
                     /translation="MSDQLRQAIADAYLADENAVSAERIAQAKLSPAEAAATHAMAST
                     LAGRLRAEGARQGSVDAFMQEYALSTEEGVALMCLAEALLRVPDAGTAEKLIRDKIAP
                     GDWDSHSGKSDSLFVNASTWALMLTGRVIRMDQSGSWELEGIFKKLVGRSGEPVIRQA
                     ITTAMRVLGGQFVLGRTIGEALRTSRSYRDKGHRISFDMLGEAAYTAPDAERHLASYR
                     KGIEAIAAAYPPDGAPVFERPSISVKLSALHPRYEWVKRERVAKELLPALADLAGRAR
                     AANIGVTIDSEEADRLEIMLDLFEALSNADDLKGWNGLGIAVQAYQKRALPELAWLIE
                     LARRQGRRIPVRLVKGAYWDTEIKRAQEAGLAGYPVFTRKAATDTSYIACARAMLAAR
                     DAVYPQFATHNAHTLSVVDVLSEGKGDLEYQRLHGMGESLYELYGELKKPSSAHAGLR
                     IYAPVGSHEDLLAYLVRRLLENGANTSFVNRMADDEAPLETLIADPVLQLANEAPRRN
                     PHIPLPEEMYLPRKNSSGFLWSDPVASAPVLAAMADALRAPAVAAPLIAGSMREGEVR
                     PLFDPSDRNRQIGQVTDATQVDAMEALIHATRACGPWDAIGGDARAAILERAADLYEA
                     NRARLMALTVREGGKTLATALGEVREAADYLRYYAAEARRYFSAPVKLPGPTGEANEL
                     TLHDKGVFACISPWNFPLAIFTGQVAGALAAGNAVLAKPAAPTPLIAAAAVALLHEAG
                     VPKEVLHLLPGSGTKLGA"
     gene            2440..2892
                     /locus_tag="VGC27_10760"
     CDS             2440..2892
                     /locus_tag="VGC27_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0283090.1"
                     /translation="MLSQKARYALRALFVLGAHDSDEPIMIADIAERANVPRKFLEQI
                     LLEMKRRGIVHSQRGKFGGYALARAPEDISFAEVLRVIDGPLALTPCASVTAYRKCDD
                     CEDETTCAIRKVLLAVRGATADILEHHTLAHALADEKATKKSRRRRKN"
     gene            3235..4764
                     /locus_tag="VGC27_10765"
     CDS             3235..4764
                     /locus_tag="VGC27_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY0283091.1"
                     /translation="MQIVSKTFAAALLAGTASLALVSGALAADKHNSDRTSAQGAGSV
                     HDAKIQALENQIQDLSNQVEDLKRSTGDQYTDVQNQQAAAVKVTLANGRPTISTADGN
                     FTASLRATVQYDTGFYFQDNAVTANLTPALGRDLSSGTNFRRAQFGLDGTVFANWSYS
                     FIYDFGGSNGAEQQGRVSAAYIQYAGLAPFQFRIGAFAPYIGLEDSTSSADLLFPERP
                     TPSELSRSNTGGDGRSAIQIAAIGDNYLASVSYSGSSAATAAVFDEQQAINGRLAYLF
                     YNDLNTKLVGSLSAVYEFDAPDSVANAPGATNITLADRPELRVDGTQLITATIDADKL
                     LTGGAEVGLVWKNLYAQGGWFTYNVSRRASTLPDPNFSGWYLQGSWILTGESKPYDAT
                     RAAFRNPRPANPLNFGDAPGIGAWELAARYSVTDLNYREALAPALGGVRGGEQKIFTA
                     GLHWYANNAIKLLFDYSHVDISRLSPSAIAASSPYPAVPLGADIGQSFSILNLRAQIA
                     L"
     gene            4800..>5091
                     /locus_tag="VGC27_10770"
     CDS             4800..>5091
                     /locus_tag="VGC27_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0283092.1"
                     /translation="MNRFSYRISTAIAAAAVLLGAGFASVGAQAAGVTLLNVSYDPTR
                     ELYKEINAAFAAQWKAKTGQDVTINQSHGGSGSQARAVIDGLDADVVTLALAY"
BASE COUNT          969 a         1676 c         1532 g          914 t
ORIGIN      
        1 ggcaccgagc tttgttccgc ttcccggcaa gagatgcagc acctccttcg gcacaccggc
       61 ttcgtgcagc aacgcaacag cggcggcggc gatcagcggc gtcggcgcgg cgggtttggc
      121 gagcaccgcg ttcccggccg ccagcgctcc ggccacctgt ccggtgaaga tggcgagcgg
      181 aaaattccat gggctgatgc aggcgaacac gcccttgtcg tgcagcgtca gctcgttggc
      241 ttcgcccgtc ggaccgggaa gcttcaccgg cgcggaaaaa tatcgccgcg cctcggccgc
      301 gtaatagcgc agatagtcgg cggcctcgcg tacttcgcca agcgccgtgg ccagcgtctt
      361 gccgccctcg cgcacggtga gcgccatcag cctggcccgg ttcgcttcgt agagatcggc
      421 ggcgcgttcg aggatcgcgg cgcgcgcatc gccgccgatc gcgtcccacg gaccgcaggc
      481 gcgcgtggcg tggataaggg cttccatggc gtccacttgc gtggcgtccg tcacctgccc
      541 gatctgccgg ttgcggtcgg agggatcgaa cagcggacgc acctcgcctt cgcgcatgct
      601 gccggcaatc agcggcgcag ccaccgccgg cgcgcgcagg gcatcggcca tcgccgccag
      661 gaccggcgcg ctggcgaccg gatcgctcca caaaaagccg gacgaattct tccgtggcag
      721 atacatctcc tccggcaagg ggatatgcgg attgcggcgc ggtgcttcgt tggccaattg
      781 caggaccgga tcggcgatca gcgtttcgag cggtgcttcg tcgtccgcca tgcgattgac
      841 gaaggaagta ttggcgccgt tctccagcaa gcggcggacg agataggcga gcagatcctc
      901 gtggctgccg accggcgcat agatgcgcag cccggcgtgc gcggacgacg gcttcttgag
      961 ttcgccgtaa agctcgtaca gggactcgcc catcccatgc agccgctggt attcgagatc
     1021 gcccttgccc tccgaaagaa catccacaac cgaaagcgta tgggcgttat gggtagcgaa
     1081 ttgcggatag accgcatcgc gtgcggccag catggcacgg gcgcaggcaa tatatgacgt
     1141 gtcggtcgcc gccttgcgcg tgaacacggg atagccggcc agtcccgcct cctgggcgcg
     1201 cttgatctcg gtatcccaat aggccccttt caccagccgc accggaatgc ggcggccctg
     1261 gcggcgcgcc agctcgatca gccaggccaa ttccggcaaa gcgcgcttct gataggcctg
     1321 aaccgcgatg ccgagtccgt tccaaccctt gagatcgtcc gcgttcgaca gcgcctcgaa
     1381 caggtcgagc atgatttcca gcctgtcggc ctcttcggag tcgatggtga cgccgatgtt
     1441 cgcggcgcgc gcccggcccg ccagatcggc gagcgcgggc aacaactcct tcgccacgcg
     1501 ctcgcgcttg acccattcat aacgcgggtg cagcgccgac agcttcaccg aaatgctcgg
     1561 gcgctcgaag accggcgcgc cgtccggggg ataggcggcg gcgatggcct cgataccctt
     1621 gcgataggac gccagatggc gttcggcatc gggcgccgtg tacgcggctt cgccgagcat
     1681 atcgaacgat atgcggtggc ctttatcgcg atagctccgc gaggttctca gagcctcgcc
     1741 gatggtccgg ccgagcacaa actggccgcc cagcacgcgc atcgccgtgg tgatggcctg
     1801 gcggatcacc ggctcgcccg aacggccgac cagctttttg aagatgcctt ccaactccca
     1861 actgccgctc tggtccatgc ggatgacgcg cccggtcagc atcagcgccc aggtggaagc
     1921 gtttacgaac agcgagtccg acttgccgct gtggctgtcc caatcgcccg gagcgatctt
     1981 gtcgcggatc agcttttcgg ccgtcccggc gtccggcacg cgcagcaacg cctccgcgag
     2041 gcacatcagc gccacgcctt cctcggtgga gagagcgtat tcctgcataa aggcgtcgac
     2101 gctgccctgc ctggcgcctt cggcgcggag ccttcccgcg agcgtgcttg ccatggcatg
     2161 cgtcgcggcg gcttcggcgg gcgaaagttt cgcctgggcg atccgttcgg cggagacggc
     2221 gttctcgtcg gcgagatagg catcggcaat ggcttggcgc aattggtcgc tcatggggtg
     2281 aagtcgctgg gtggcgaatc cattctagtg tcccgattcc aaaattcgca ccatctttat
     2341 atcggcttcg ggcgaatttc ggaatcgaaa ggacactagc agtattgaac ctgcttctat
     2401 attcactata acatctgtag aattaatagg tgaaccggca tgttgtccca aaaagcccgt
     2461 tatgcgctgc gcgccttgtt cgttcttggg gcgcatgaca gcgacgaacc gataatgatc
     2521 gcggacatcg ccgagcgggc caacgtgccg cgcaagttcc tcgaacaaat cctgctggaa
     2581 atgaaacggc gcggcatcgt gcacagccag cgcggcaagt tcggcggcta cgcgctggcg
     2641 cgcgcgccgg aagacatcag ctttgccgaa gtgctgcgcg tgatcgacgg accgctggcg
     2701 ctcacgcctt gcgccagcgt cacggcctac cgcaaatgcg acgattgcga agacgaaacg
     2761 acctgcgcaa ttcgcaaagt ccttctcgcc gtgcgcggcg cgaccgccga catcctcgag
     2821 catcacacgc tcgcacacgc tctcgccgac gagaaggcca cgaaaaaaag ccggcggcgt
     2881 cgcaagaatt aattctagag cgcaatccgg caaagtgtga agcggtttgc cgtcagattg
     2941 cgcgaaaatc aaggaatcta gagcttgatc cgattcaatt aaatcggatc aagctctaga
     3001 aactgttctc accaaaattc ctcgtctgcg gctttgcaag cgagccgcaa aattgtacca
     3061 gtagagtttg ctaaaatatt atttcaacaa aaaatattca taatctatat tgactaggta
     3121 gggattgtag tgatgatgca tttgtggccc gggggtcgcg ccacatactc cggacggatc
     3181 gggggctcct ccccgcctcg atcccgtccg gaggccagac caggaggtgg agtcatgcaa
     3241 atcgtgtcga agacatttgc ggccgcgctg ttggccggaa cggcgagcct tgcgcttgtc
     3301 tccggcgcac tggccgcgga caaacacaac agcgaccgga catcggcgca aggcgccggc
     3361 agtgtccacg acgccaagat ccaggcgctc gaaaatcaga tccaggattt gtcgaaccag
     3421 gtggaggacc tcaagcgcag caccggcgac cagtacaccg acgtgcaaaa tcagcaagcg
     3481 gccgcggtca aggtgactct cgccaacggc cgcccgacga tttccaccgc cgacggaaat
     3541 ttcaccgcct cgttgcgggc gaccgtgcaa tacgacacgg gattctactt tcaggataac
     3601 gccgtcaccg cgaatctgac gcccgcgttg ggccgcgatt tgtcttcggg cacgaatttc
     3661 cgccgcgcgc aattcggcct cgacggcacg gtgttcgcga actggagcta tagcttcatc
     3721 tacgatttcg gcggcagcaa cggcgccgaa caacagggcc gcgtttccgc ggcctacatc
     3781 caatatgccg gcctcgcgcc gttccaattc cgtatcggtg cctttgcacc ctatatcggg
     3841 cttgaagact cgacctcctc cgccgatttg ctgtttcccg aaaggccgac gccatccgag
     3901 ctttcgcgct ccaataccgg cggcgacggg cgctccgcga tccagatcgc ggcgatcggc
     3961 gataactatc tcgcctcggt cagctacagc ggttccagcg ccgccaccgc cgccgtgttc
     4021 gacgaacagc aggcgatcaa cggacgcctc gcctatctct tctacaatga tctcaacacc
     4081 aaactcgtgg gcagcctttc ggcggtctat gaattcgatg cacccgactc ggttgcgaac
     4141 gcgccgggcg ccaccaacat caccctcgcc gaccggccgg aattacgcgt ggacggcacc
     4201 caactcatca ccgccaccat agacgccgac aagcttctga ccggcggtgc ggaagtgggc
     4261 ctcgtatgga agaacctcta tgcccagggc ggctggttca cctataacgt ctctcgccgc
     4321 gccagcacgc tgcccgatcc gaatttctcc ggctggtatc tgcaaggaag ctggatcctg
     4381 acgggcgaat ccaagcccta cgacgcgacc cgcgcggcat tccgcaatcc caggccggcc
     4441 aacccgctga atttcggcga cgcccccggc atcggcgcat gggaattggc ggcacgctac
     4501 agcgtcaccg acttgaacta ccgcgaggcg ctggcgcccg cgctgggcgg cgtgcgcggc
     4561 ggcgagcaga agattttcac cgcaggtctg cactggtacg caaataacgc catcaagctg
     4621 ctgttcgatt attcccatgt ggatatcagc cggctaagcc ccagcgccat cgcggcttct
     4681 tcaccttatc cggccgtccc cttgggcgcc gatatcggcc aaagcttcag catcctcaat
     4741 ctccgggcgc aaatcgccct ctaacgcgct ataatcccga cgagaatgga gttcccacaa
     4801 tgaaccgctt ctcttaccgc atctcgaccg ccatcgccgc cgccgccgtt ctcttgggag
     4861 ccggctttgc gagcgttggc gcccaggccg ccggcgttac gctgctgaac gtcagctacg
     4921 atccgacgcg cgaactctac aaggagatca acgccgcctt cgccgcgcaa tggaaggcca
     4981 agacgggtca ggacgtcacg atcaaccaat cgcacggcgg atcgggatcg caagcgcgcg
     5041 ccgtgatcga cgggctcgac gccgacgtcg ttaccctggc gctggcctac g
//
LOCUS       DASWPU010000507         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_102001,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000507 DASWPU010000000
VERSION     DASWPU010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102001"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..>1904)
                     /locus_tag="VGC27_10775"
     CDS             complement(66..>1904)
                     /locus_tag="VGC27_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0283093.1"
                     /translation="DEDLEKGAKPGNSALEAYCVNLNEKARQGRIDPLIGREAEVERT
                     IQILCRRQKNNPLFVGDPGVGKTAIAEGLARKIVKNDVPEVLRNATIFALDMGALIAG
                     TRYRGDFEERLKSVIKEMEAVPGAILFIDEIHTVIGAGATSGGAMDASNLLKPALQSG
                     TLRCMGSTTYKEYRQYFEKDRALVRRFQKIDVAEPTVPDTIKILKGLKPYFEEYHKVR
                     YTADAIKAAVELAAKYINDRKLPDKAIDVIDEVGASQMLLPESRRKKVIGVKEVEEVI
                     AKMARIPPKSVSKTDAEALRTLGDDLKRVVFGQDTAINALSSAIKLARAGLRGPEKPI
                     GCYLFSGPTGVGKTEVAKQLASIMGVELLRFDMSEYMERHTVSRLIGAPPGYVGFDQG
                     GLLTDGVDQHPHCVLLLDEIEKAHMDLFNILLQVMDHGKLTDHNGKKIDFRNVVLIMT
                     TNAGASDAARESIGFGRGKREGDDEEAIKKLFTPEFRNRLDATIAFAALSRDSIDRVV
                     EKFVFELEAQLIDRDVTFDLTPEATRWLGDKGYDDAFGARPLARVIQDSIKKPLADEI
                     LFGKLQNGGTVRVLLDRGSDKLVFEFITEESKPKQLPPPEKSEENV"
BASE COUNT          306 a          598 c          592 g          408 t
ORIGIN      
        1 acccacgttt gctccccctc actacgccgc agcgaggaga cacattacgc agagccaaca
       61 gacaattaca cgttttcttc gcttttttcc ggcggtggaa gctgtttcgg cttcgactct
      121 tcggtgatga attcgaacac cagcttgtcg gagccgcgat ccaaaagcac gcggacggtg
      181 ccgccgttct gcaacttgcc gaacaggatt tcgtcggcca gcggcttctt gatggaatcc
      241 tggatgacgc gcgccagcgg ccgcgccccg aaggcatcgt catagccctt gtcgcccagc
      301 cagcgcgtcg cttccggcgt caggtcgaag gtcacgtcgc ggtcgatcaa ctgcgcttcg
      361 agttcgaaca cgaatttttc caccacgcgg tcgatgctgt cgcggctgag cgccgcgaag
      421 gcgatggtcg cgtccagccg attgcggaat tccggcgtga acagcttctt gatcgcttcc
      481 tcgtcgtcgc cttcgcgctt gccgcggccg aagccgatgg actcgcgcgc ggcgtcggag
      541 gcgcccgcgt tggtcgtcat gatcagaacc acgttgcgga aatcgatctt cttaccgttg
      601 tggtcggtca gcttgccgtg atccatcacc tgcaacagga tgttgaacag gtccatgtgc
      661 gccttctcga tttcgtcgag cagcagcacg caatgcggat gctggtccac cccgtcggtg
      721 agcaggccgc cctggtcgaa gccgacatag cccggcggcg cgccgatcag ccgcgaaacc
      781 gtgtggcgct ccatatattc gctcatgtcg aagcgcagaa gttccacgcc catgatcgag
      841 gcgagctgct tggcgacttc ggtcttgccg acgccggtgg ggccggagaa cagatagcag
      901 ccgatgggct tttccgggcc gcgcagaccg gcgcgcgcca gcttgatcgc cgacgacagt
      961 gcattgatcg cggtgtcctg gccgaacacc acgcgcttga gatcgtcgcc cagggtgcgc
     1021 agcgcctcgg catcggtctt ggagacggat ttgggcggaa tgcgcgccat cttggcgatc
     1081 acctcttcca cctctttgac gccgatcacc ttcttgcggc gcgactccgg cagcagcatc
     1141 tgcgaagcgc ccacctcgtc gatcacgtcg atggccttgt cgggcagttt gcggtcgttg
     1201 atgtatttcg cggcgagttc gaccgccgcc ttaatggcgt ccgcggtgta gcggaccttg
     1261 tggtattcct cgaaataggg cttcaggcct ttcaggattt tgatcgtgtc gggcacggtc
     1321 ggctcggcca cgtcgatctt ctggaagcgg cgcaccaaag cgcggtcctt ctcgaaatac
     1381 tggcgatatt ccttgtaggt ggtggaaccc atgcagcgca gggtgcccga ctgcagcgcc
     1441 ggcttgagca gattggaagc gtccatcgct ccgcccgagg tggcgcccgc gccgatcacc
     1501 gtatggattt cgtcgatgaa caggatcgcg ccggggacgg cttccatctc cttgatgacc
     1561 gatttgagcc gctcctcgaa atcgccgcga tagcgcgtgc cggcgatcag cgcccccatg
     1621 tcgagggcga agatcgtggc gttgcgcagg acttccggca cgtcgttctt gacgatcttg
     1681 cgcgccaggc cctcggcgat ggcggtcttg ccgacgccgg ggtcgccgac gaacagcgga
     1741 ttgttcttct gccggcggca gaggatttgg atggtgcgtt cgacttccgc ttcgcgcccg
     1801 atcaagggat cgatgcggcc ctgcctggcc ttttcgttca gattgacgca ataggcttcg
     1861 agcgcgctgt tgcccggttt ggcgcctttt tccaggtctt cgtc
//
LOCUS       DASWPU010000508         4168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_53168,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000508 DASWPU010000000
VERSION     DASWPU010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53168"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..>4168
                     /locus_tag="VGC27_10780"
     CDS             53..>4168
                     /locus_tag="VGC27_10780"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY0283094.1"
                     /translation="MQRSIDGHLRDATRTAEYAQLLNLRLLALETTARSMAEAMEFGF
                     LLDPHRKLLSIGYLAVEGRLDESCYDLLASEARLASFVAIAKGDIPSRHWFRLGRIVT
                     PIEHGAALISWSGSMFEYLMPSLVMRAPAGSLLEETSRLIVHRQMSYGAERKLPWGIS
                     ESAYNARDLELTYQYSNFGVPGLGMKRGLGANAVVAPYATALATMVDPAAAVRNFIRL
                     AAAGARGRYGFYEALDYTRARLPRHKDCAIIRAFMAHHQGMTIVAIANALLDGEMRAR
                     FHAEPAIQATELLLQERTPRDVAAARPWVEDAKTSVAVRVFDRPTVRRIRSPFGPTPA
                     VHLLSNGRYAVMLTAAGSGYSRWRDLGITRWREDTTCDDWGSYIFLRDVRSGNVWSAG
                     HQPSGAEPDSYETRFSEDKVEYRRRDGAIATVLEVVVSPEDDAEVRRLSISNGSNRPR
                     EIEITSYAELVLAPRAADAAHPAFSKLFVQTEYDAKAGVILATRRRRSPTEPEIWAAH
                     LAVVEGETVGVTEIETDRAKFLGRGRDARSPIAMMDKRPLSNSVGTVLDPIFALRRRV
                     RVQPGETVRIAFWTIVASSRADLLDLVDKHQDANAFDRAATLAWTQAQVQLRHLDIDA
                     EKASLFQRLAGHVLYASPSTRPSSDIIRRGSGGPAGLWAQSISGDIPIVLVRIASIED
                     IAVIGQLLRAHEYWRLKRLAVDLVILNERSSSYVQDLHVAVETMVRTSQSRPQAGLGV
                     TRGGVFVLRADLISQETRTLLLSVARVVLAARQGSLSDQLDRLRKASITAPPPLRRLK
                     PTELPPPILAIPDLEFFNGLGGFAADGREYVTVLKAGQVTPAPWTNVIANASFGFQVA
                     AEGGGYTWSVNSRENQLTPWSNDPVADRPGEVLFVRDVDSGVLWGPTALPIRDEAAPY
                     IVRHGQGYSRFEHDAHGIKLDLLQYVPLHDPIKISRLKIHNISQRTRRLSVTAYVEWV
                     LGPSRSASAPFIATETDPETRALFARNPWNTTFGSRVAFADLAGRQTRWSGDRREFLG
                     RNGSLDNPGALAADAPPLSGRVGAGLDPCGVLQTPVTLAPGQSIEIVFFLGEADSAAD
                     ARFLITRYRAIDLNTVFQDVLQHWDDLLGTVQVKTPDRSMDIMLNRWLLYQTVACRLW
                     ARSAFYQASGAYGFRDQLQDGMALALPRPAMTREHLLRAAARQFTPGDVQHWWLPPFG
                     QGVRTRISDDHIWLAYAVAHYVETTGDLAVLDEQVPFIEGQSLQSGEADAYFQPTVSN
                     QAASLFEHCARGLDQSLPVGAHGLPLIGTGDWNDGMTRVGDQGKGESIWLGWFLYATL
                     SAFAPLANARQEQARAAKWRSHAQALQTALEQDGWDGNWYRRAYFDDGTPLG"
BASE COUNT          737 a         1371 c         1288 g          772 t
ORIGIN      
        1 gctggccagc ggggatgaag ccagtctcga aatgctattc tggacacggg cattgcagag
       61 atcgatcgac ggtcacctac gcgacgcgac ccggaccgcc gaatacgccc agttgttgaa
      121 cctgcgcctt ctcgcgctcg agacgacggc gcgctccatg gcggaagcaa tggagttcgg
      181 cttccttctc gacccgcatc gcaagctcct ctcgatcggc tatctcgccg tggagggccg
      241 tctcgacgaa agctgttacg acctgctggc ttccgaagcg cgcctcgcga gcttcgtggc
      301 gatcgccaaa ggcgacattc cgtcccggca ctggttccgg ctcggacgca tcgttacccc
      361 gatcgagcat ggggcggcgc tgatctcctg gtcggggtcg atgttcgagt atctgatgcc
      421 gtcgctggtg atgcgcgctc cggccggcag tttgctggaa gagacaagcc gtctcatcgt
      481 ccaccggcaa atgagctatg gcgccgagcg gaaactgcct tggggcattt ccgaatccgc
      541 ctacaacgcg cgcgacctgg aattaaccta tcaatattcg aatttcggcg taccggggct
      601 ggggatgaaa cgcggcctgg gcgcgaatgc cgtggtcgcg ccttatgcaa cggccctcgc
      661 cacaatggtc gaccccgcag ccgccgtgcg caacttcatc cggcttgccg cagccggcgc
      721 gcgcggccgc tatggcttct acgaggcgtt ggactatacc cgcgcgcgtc tgcctcggca
      781 taaagactgc gcgatcatac gcgccttcat ggcgcaccat cagggcatga cgatcgtcgc
      841 catcgccaat gcgcttctgg atggagaaat gcgggcacgc tttcatgcag aaccggccat
      901 ccaggcgacg gaactcctct tgcaggagcg cacaccgcgc gatgtcgcgg ccgcccggcc
      961 ctgggtggag gacgccaaga cgagtgtcgc ggtccgcgtg ttcgatcgcc cgacggtgcg
     1021 gcgcattcgc tccccgttcg gcccgacgcc ggcggtacac ctgctctcca acggccgtta
     1081 tgcggtgatg ctcacggcgg ccggttccgg ctacagccga tggcgcgacc tgggaattac
     1141 gcgctggcgc gaagatacta cctgcgacga ctggggttcg tacatcttct tgcgggacgt
     1201 gcgcagcggc aacgtatggt ccgcgggcca tcagcccagc ggcgccgaac cggatagtta
     1261 cgaaaccagg ttcagcgagg acaaggtaga gtacagaagg cgcgacggag caatcgcgac
     1321 agttctcgag gttgtggttt caccggaaga cgatgccgag gttcgccgcc tgtccatctc
     1381 gaatgggagc aatcggcccc gcgagatcga gattacctcc tatgccgagc ttgtgctggc
     1441 tcctcgggcg gccgacgcgg cgcatccggc cttttcgaag ctgttcgtgc aaaccgaata
     1501 tgacgccaag gccggcgtca tcctggcgac ccggcgccgc cgctctccga ccgaacccga
     1561 gatctgggcg gcgcatctcg ccgtagtcga aggcgagacg gtcggtgtga ccgagattga
     1621 aaccgaccgt gccaagttcc tcggccgcgg gcgcgatgca aggtcgccga tagcgatgat
     1681 ggacaaacgg cccctctcca acagcgtcgg aaccgtgctc gaccccatat tcgctttacg
     1741 ccgccgcgtg cgggtgcagc cgggcgagac ggtgcgtatc gcattctgga ccatcgtcgc
     1801 gtcatcccgc gccgacctgt tggacttggt cgacaaacat caggatgcca atgccttcga
     1861 tcgcgcggcc acgctggcct ggacccaggc gcaggtgcag ctgcgccatc ttgatatcga
     1921 cgccgaaaag gccagtctct ttcagcgcct tgccgggcac gtgctctatg ccagtccgtc
     1981 cacgcgtccg tcttccgaca tcattcgcag aggcagcggc ggccccgcgg gattgtgggc
     2041 tcagagtatt tccggcgata ttccgatcgt gctggtgcgg atcgcctcta ttgaggacat
     2101 cgccgtcatc ggccaattgc tgcgtgccca cgagtattgg cggttgaagc ggctggccgt
     2161 cgatctggtg atcctcaacg aacggtcatc ctcctacgtc caggatcttc atgtcgccgt
     2221 cgaaacaatg gtgcggacaa gccaatcccg tcctcaggcc ggcttgggcg ttacgcgcgg
     2281 cggtgtgttc gtgctgcgcg ccgatctcat ctctcaggag acgcgcacgt tgctcctatc
     2341 cgttgcgcgg gtcgtgctgg cggcccgcca aggcagcctg tcggaccagc tcgaccgctt
     2401 acgcaaagca agcattaccg cgccgcctcc cctgaggcgc ttgaaaccaa cggagctacc
     2461 gccgcccatt ctcgcaatac ctgacctcga gttcttcaac ggcctcggcg gcttcgccgc
     2521 cgacggccgg gaatatgtaa ccgtcctgaa agccggacag gtgacgccgg cgccgtggac
     2581 caatgtgatc gccaacgcat cattcggttt ccaggttgcg gcggaaggcg gcggttacac
     2641 atggtccgtt aacagccggg aaaaccagtt gacgccttgg tcgaacgacc ccgtggccga
     2701 ccggccgggt gaagttctct ttgtccgcga cgtggacagc ggtgtgttgt ggggaccgac
     2761 cgccctgccg atccgggacg aggccgcgcc ctatatcgtc cgccacggtc agggatacag
     2821 ccggttcgaa cacgacgcgc acggcatcaa actcgatctg ctccagtacg ttcctcttca
     2881 cgatccgatc aagatttcgc gcctgaaaat ccacaatatc tctcagcgga cccggcggct
     2941 ctcggtcacg gcttatgtcg aatgggtgct cggtccgtcg cgcagcgcat ccgctccctt
     3001 catcgcgacg gaaaccgatc cagaaacccg cgcgctcttt gcccgcaacc cctggaacac
     3061 caccttcggt tcacgggtcg cgttcgcgga cttggccggc aggcagacac gctggagcgg
     3121 agaccggcgg gagtttctgg gccgaaacgg atcgctcgac aatcctggcg cgctcgccgc
     3181 ggacgcacct ccgctttcag gacgagtcgg cgcaggcctc gatccgtgcg gcgttcttca
     3241 aaccccggtg acgctcgcac cgggccaaag catcgagatt gtctttttcc ttggtgaagc
     3301 agacagcgcg gccgacgcgc gatttctgat cacgcgttac cgcgcgatcg atctcaacac
     3361 ggtttttcag gacgtcttgc agcattggga cgatttgctc ggcaccgtgc aggtgaagac
     3421 gccggaccgt tcgatggaca tcatgctcaa ccgctggctg ctctatcaaa cggtagcctg
     3481 ccgcttgtgg gcgcgatcgg cattctatca ggcgagcggc gcctatggct tccgcgatca
     3541 gctccaggac ggaatggcgc tcgcgctacc gaggccggcg atgacgcgcg aacatctttt
     3601 gcgcgccgcc gcccgccagt tcacaccagg cgacgtccag cattggtggc tgccgccgtt
     3661 cggtcaaggc gtccgcacac gcatttcgga cgaccatatc tggctggctt acgccgtcgc
     3721 gcactatgtc gagaccacgg gcgatcttgc cgtgctggac gagcaggttc ccttcatcga
     3781 agggcaaagc ctccagtccg gtgaagccga cgcctatttc caaccgaccg tatccaacca
     3841 agccgcctcg ctgttcgagc actgcgcgcg gggcctcgat caaagccttc cggtcggcgc
     3901 acatggactg cccttgatcg gcaccggcga ctggaacgac ggcatgaccc gtgtcggcga
     3961 ccaggggaag ggtgaaagca tctggctcgg ttggttcctc tacgcgacat tgtccgcctt
     4021 cgcaccgctg gccaacgcgc gccaggagca agcgcgcgcc gccaaatggc gttcgcacgc
     4081 ccaggcgctg caaacggccc tcgaacaaga cggttgggac gggaactggt accggcgcgc
     4141 ttattttgac gacggaacgc ctctcggc
//
LOCUS       DASWPU010000509         5372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_108794,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000509 DASWPU010000000
VERSION     DASWPU010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108794"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VGC27_10785"
     CDS             <1..1086
                     /locus_tag="VGC27_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283095.1"
                     /translation="DPNWIAPLLLERQIHFDRSRNPFFRHAKAAFWLAYRGEVPVGRI
                     TAQIDALHLERHHDATGHFGFIEGVDDPAVFEALFGAAEAWLRAEGMRRSVGPISFSM
                     WDEPGLLVEGFDRPPNVLMGHALPYYQRQIAACGYSQVQDLLAYEYPVNQPFSENVQR
                     IIAQAQRKRHFRFRPMRMDRKNFASEIELILDIMNDAWSENWGFVPMTRAEVDDMAAL
                     FKFVLRPDAVVIAEYDGEAAGFGMMLPNVNEMIKDLKGRLFPFGFAKLLWRLKVSPLR
                     SGRMALMGVRRKWWSSPVGAIVALLIIQQAKISDFARDGVYAELSWILDSNERVKRVV
                     ALFGASVIKRYRIYEKALGGNSDVGGH"
     gene            complement(1066..2301)
                     /locus_tag="VGC27_10790"
     CDS             complement(1066..2301)
                     /locus_tag="VGC27_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0283096.1"
                     /translation="MPSDQSLVAYANRRARLAGMGLLESANPWFVPLDRLQAAVSGEG
                     GTLVSFANYDYLGLAGHPAIAEAAHSALDKVGVGALGSRLVGGERLIHADFEEALARF
                     VGADACLTLVSGYLTNLTTISHLMGRRDLILYDELSHNSIIAGVTTCKAASIEFKHND
                     MEHLQSLLKESRANYRNCLIVVESLYSMDGDIANLPELLALKDEFGCWLLVDEAHSIG
                     VLGKNGRGLSEHFGEDPKRIDIIIGTLSKTFASCGGFICAQKPVLDWMRFTLPGFVYS
                     VGLPPVIAAAGQAALELIVAEPQRVSRLQGNARYFLDKAQEAHLETGPAEGHAILPVI
                     FPDMKSTMECADFLLKKGIYAPPIVQVSVPKGLPRIRFFISARHTADDIDRTVSAIKS
                     FVAAHPAGARQAASVAAHV"
     gene            complement(2616..3641)
                     /locus_tag="VGC27_10795"
     CDS             complement(2616..3641)
                     /locus_tag="VGC27_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283097.1"
                     /translation="MDIACAGIAAVQSACLALRHWGERMFVQIWRNLECAVARLNARW
                     GRAALHLLRWAIPILLLAYLGYSLTRLGWAQVWNARPQNLGFYLAVLLPFYVQPVADL
                     VIYHNLLGIGRVLPLFVILRKRFVNTMLDYSGEVYFFFWAKKNLELKQGVLLHAVKDT
                     NILSASAGLAMVWLTLLAVMASGSLKLPALVHDSFWTFVFFGSLPLILGLALFIGGRR
                     VTALSRGDVVTTFGIHFARCATQLSLEFMILWLSGALPSAAICIRFVALRVVLTRLPL
                     VPNKDLVFVGVGIAAAGLMGVSTQSVAAMLVLMTAAGLLQNLLLVGLPWFLEQFQVGH
                     RVRAAAS"
     gene            complement(3653..4408)
                     /locus_tag="VGC27_10800"
     CDS             complement(3653..4408)
                     /locus_tag="VGC27_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0283098.1"
                     /translation="MVAKNILITGASSGIGAALARFYAAPESRLVLWGQNAERLAVTA
                     EQCRARGAAFETACFDLTDFGQLVRALEETDARSPLDLAFFNAGLGGSLPRDRAAQDA
                     QAAERMAGVNFTAPVMAANLIAERMAERGRGRIVLIGSVAASFPLPMAPVYAGSKAGL
                     ALFAEALGLRLAKYGVGVTLVSPGFVDTPMSRGLKEPRPFLIKPDAAAAIIAGKVERG
                     VRHIVLPWQFAVLRAAAMLVPRPIMRAVLSRLL"
     gene            4533..>5372
                     /locus_tag="VGC27_10805"
     CDS             4533..>5372
                     /locus_tag="VGC27_10805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0283099.1"
                     /translation="MSVQSHSHGERRDNDGARATDSGNGPAGRMSGENKKRPLRVALV
                     ASSYNYIRDGVALTLNRLVGYLEQQGIEVLVFAPVGKIAALAHNGTLVPVPSVALPPR
                     PEYRFAFPLPRQAIDRLRAFRPDIMHIALAPDLLGYSALRAARRLKIPVVASCHTRFE
                     TYLKHYWYSVWLGGLLKAYLRHAYGACREVYVPSQSMIDALLADGMKDNFRLWPRGVD
                     TAQFHPGKRSGHWRAKHGIGESEFVVAFVSRLVREKELDTLVAAVRRLETRGTAHRVL
                     IVGD"
BASE COUNT         1111 a         1666 c         1639 g          956 t
ORIGIN      
        1 gatccgaact ggatcgcgcc gcttcttctc gaacggcaaa tccacttcga ccgatcccgc
       61 aatccgtttt tccggcatgc gaaggcggcg ttctggctcg cctatcgcgg agaggttccc
      121 gtcggccgga tcacggcgca gatcgatgcc ctgcatctgg aacgccatca cgatgccacg
      181 gggcatttcg ggttcatcga aggagttgac gatccggccg tgttcgaggc cttgttcggc
      241 gcggccgaag cgtggctgcg ggcggagggc atgcgccgct cggtcggccc gataagcttt
      301 tccatgtggg acgaacccgg gctattggtc gagggcttcg atcggcctcc caatgtgctg
      361 atgggacacg cgctgcctta ctatcaaaga cagatcgcgg cgtgtggcta cagccaagtg
      421 caggatctgc tggcctacga atatccggtt aatcaaccgt tttcggagaa tgtgcagcgg
      481 atcatcgccc aggcgcagcg aaagcgacat ttccgctttc ggccgatgcg catggaccgg
      541 aagaacttcg cctccgaaat cgaactgatc ctcgacatca tgaacgacgc ctggtcggaa
      601 aattgggggt ttgtcccgat gacgcgggcc gaagtcgacg atatggccgc gctcttcaag
      661 ttcgtgcttc gtccggatgc cgtcgtcatc gccgagtacg acggcgaagc ggccggtttc
      721 ggcatgatgc tgccgaacgt caacgagatg atcaaagacc ttaaaggacg cctctttcct
      781 ttcggctttg ccaagctgct ctggcgcctg aaggtttccc ccctgcgctc gggacgaatg
      841 gcgctgatgg gcgtacggcg caaatggtgg agttctccgg tcggggccat cgtcgccctt
      901 ttgatcatcc aacaggcaaa gatcagcgat ttcgcgcgcg acggcgtata tgcggaactg
      961 tcgtggatcc tcgattcgaa cgagcgcgtg aagcgcgtcg tggcgctttt cggcgcaagc
     1021 gtcatcaaac ggtaccggat ttacgaaaag gcgctgggcg gaaattcaga cgtgggcggc
     1081 cactgacgcc gcttggcgtg cgccggcggg atgcgccgcg acaaaactct taatcgcact
     1141 taccgtgcga tcgatatcgt ccgccgtgtg ccgggctgag atgaaaaacc ggatgcgcgg
     1201 caagcccttc ggcacactca cctgaacgat cggcggcgca tagatgcctt tcttgagaag
     1261 gaaatccgcg cattccatgg tgcttttcat atcgggaaag atcaccggaa ggatggcgtg
     1321 accttcggca ggacccgttt ccaaatgcgc ttcctgggcc ttgtcgagga aataacgcgc
     1381 attgccctgg agcctcgaca cccgttgggg ctcggccacg atcaattcca gcgccgcctg
     1441 gccggcggcc gcgatgaccg gcggcaatcc gacactgtag acgaaaccgg gcagcgtgaa
     1501 gcgcatccag tcgagcaccg gcttttgcgc gcagatgaag ccgccgcagg aggcaaaggt
     1561 cttcgacagc gttccgatga tgatatcgat gcgcttcgga tcctcgccga aatgctcgct
     1621 caacccgcgg ccgttcttgc caagcacgcc gatcgaatgc gcctcgtcga ccagaagcca
     1681 gcagccgaac tcgtccttta gagcaagcag ctcgggaaga ttggcgatgt ccccatccat
     1741 gctgtagaga ctttcgacca cgataaggca gttgcggtaa ttcgcgcgcg attccttcaa
     1801 cagcgactgg agatgctcca tgtcgttgtg cttgaactcg atggacgcgg ctttgcaggt
     1861 cgtgacgccg gcaatgatac tgttgtggct gagctcgtcg tagagaatga gatcgcgccg
     1921 gcccatgaga tgggagatcg tcgtcaggtt cgtcagatat ccgctcacca gcgtgaggca
     1981 ggcgtccgcg ccgacgaacc gcgccagcgc ctcttcgaaa tcggcatgga tcaggcgctc
     2041 gccaccgacc aagcgggacc ccagcgcacc gacgcctacc ttgtccagcg ccgaatgagc
     2101 ggcctcagcg atcgccgggt gaccggcgag gccgagataa tcataattcg cgaagctgac
     2161 gagtgttccg ccttccccgc tgaccgcagc ttgcagccgg tccagcggta cgaaccaggg
     2221 gttggcgctc tcaagaagcc ccattccggc caggcgcgcc cgtcgatttg cataagccac
     2281 cagcgattgg tcagacggca tcccctgggc ccccttttca tctcgtcaat atgataacgc
     2341 caaggatagg aaagcaaggc ccgatgttgc ggcccacgaa cgcgctaacc cgcacaatcg
     2401 caagacaaaa ccacggtgta cagtgttatt tggtgcaacc caagcggcgg tgtcggccgc
     2461 gatgatcgca atcatgtgcg acgagagaaa acgcacgcac cggcacattc tccggtagga
     2521 atacattctg cgcaaatttt ttcgcgcgtc gctatggcga ctggtggcag cgcgggatcg
     2581 ccgcaccaac gcttcgttca gcgcggatcg cccgtttacg atgcggctgc acgcactctg
     2641 tggccgacct ggaattgttc gaggaaccac ggcaatccca ccagcagaag attctggagc
     2701 agacccgccg ccgtcatgag caccagcatg gccgctacac tttgggtaga gacgcccatc
     2761 aaccccgcag cggcaattcc cactccgacg aacacaagat ccttgttcgg caccagcggc
     2821 agtctcgtga gcaccacgcg taaagcaacg aaccggatac agatcgccgc cgaaggcagt
     2881 gccccggaaa gccacaaaat cataaattcc agcgaaagtt gcgttgcgca gcgcgcgaaa
     2941 tggatgccaa acgtcgtcac gacatcgccg cgactaaggg ccgtgaccct gcggccgccg
     3001 atgaacaacg ccagacccag gatgagcggc agcgagccga agaacacgaa cgtccagaaa
     3061 ctgtcatgca cgagtgcagg cagtttcaag ctgccgctcg ccataacggc gagcagcgtt
     3121 aaccacacca tcgccagacc cgcacttgcg gaaagaatgt tcgtgtcctt gaccgcgtgc
     3181 aacaggacgc cctgcttcaa ttccaggttc tttttggccc agaagaagaa atagacctcg
     3241 ccggagtaat ccagcatggt gttgacgaat ctcttgcgga gaatgacgaa aaggggaagt
     3301 acccggccga tgccaagcag attgtggtaa atgacgagat cggcgacggg ctgaacatag
     3361 aatggcagca ataccgcgag gtagaaaccc agattctggg gtctggcgtt ccagacctgc
     3421 gcccagccca gccgcgtcag gctgtagccc agatatgcga gcagcaggat ggggatcgcc
     3481 cagcgcagca gatgcagcgc cgctctcccc cagcgggcgt tcagcctggc aacggcgcac
     3541 tcaagattgc gccatatctg cacgaacatc cgctcccccc agtggcgcaa agccaggcaa
     3601 gccgactgta cagcggctat tccggcgcag gcaatgtcca atcgggcgcg gcctataaaa
     3661 gccgcgaaag aactgcacgc atgatcgggc gcggcaccag cattgcggcg gcgcgaagga
     3721 cggcaaactg ccagggtagg acgatatgcc gtacgccgcg ctcgaccttc cccgcaatga
     3781 tggcggcggc agcatccggt ttaatgagaa acggcctggg ctccttcagg ccgcggctca
     3841 tcggcgtgtc gacaaagccc ggagaaacca gcgtcacacc cacaccgtat ttggcgagcc
     3901 gcaagccaag cgcttccgcg aacagcgcca gccccgcctt gcttcccgcg tagacgggcg
     3961 ccatcggcag cgggaaggag gcggcgacgg aaccgatgag aacgattcgc ccgcggccgc
     4021 gctcggccat gcgttccgca atcaggttcg ccgccatgac gggtgcggtg aagttcacac
     4081 cggccattct ttccgccgct tgcgcgtcct gcgcggcacg gtcgcgcggc aggctgccgc
     4141 caagtcccgc attgaagaag gcgagatcga gcgggctgcg tgcgtcggtt tcctcaaggg
     4201 cccggacgag ttggccgaaa tcggtgagat cgaagcacgc ggtctcgaac gcggccccgc
     4261 gcgcgcggca ctgttccgcc gtgacagcaa ggcgctcggc gttttgcccc cacaagacaa
     4321 gccggctctc cggcgcggca taaaaacggg cgagcgcggc gccgatgccg ctggaggcac
     4381 ccgtgatcag gatgttcttg gcgaccattg gttccacgga gtcataaaaa cgattaatgc
     4441 aagcgcgttc ggcatgaatc ccccccgcac ggcacgttga caaagacatg cctgcgcctt
     4501 cttgtaaaga tcaccctgag aggcagcttg tggtgtccgt tcaatcacat agccacggcg
     4561 aacggcgcga caacgatggc gctcgcgcga cggattcggg aaacggaccg gccggccgga
     4621 tgtcgggcga aaacaaaaaa cggccgttgc gcgtggccct tgtggccagt tcctacaact
     4681 acatcagaga cggcgttgcg ttgacgctga accggctggt tgggtatctg gaacaacaag
     4741 gtatcgaggt gctcgtcttt gcgcccgtgg gcaagattgc ggccctcgcg cacaacggta
     4801 cgctggtgcc cgtgccgtcg gtagccttgc cgccgcggcc ggaatatcgc ttcgccttcc
     4861 ccctgccgcg gcaagcaata gatagactcc gcgccttccg tccggacatc atgcacattg
     4921 cactggcgcc ggacctgtta ggctacagcg ccctgcgggc ggctcgacgg ttgaaaatcc
     4981 cggtggtcgc gtcctgccac acgcgattcg aaacctatct caaacattat tggtattccg
     5041 tctggctggg cgggctgctt aaagcctatt tgcgccacgc ctacggcgcc tgccgcgagg
     5101 tctatgttcc atcgcagtcc atgatcgatg cgctgctggc ggacggcatg aaggacaatt
     5161 tccgcctctg gccgcgcggc gtggacacgg cgcaattcca tcccggcaag aggtcgggcc
     5221 actggcgcgc gaagcatggc atcggcgaga gcgaattcgt cgtcgccttc gtctcccggc
     5281 tggtgcgcga aaaggaactc gacacgctgg tcgcggcggt gcggcggctg gaaacgcgcg
     5341 gtaccgccca tcgcgtcctg atcgtgggcg ac
//
LOCUS       DASWPU010000510         4390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_47364,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000510 DASWPU010000000
VERSION     DASWPU010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47364"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VGC27_10810"
     CDS             <1..310
                     /locus_tag="VGC27_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283100.1"
                     /translation="TVFYDKDGIELRVAVNWRDTYLDRFGHGQNGGTSFGSEPIFVNG
                     NWDLTVGGGYQITDNIKAYFTANNLLDTVYSTRGRFPDQVYGVITIGRSFTAGVHYRM
                     "
     gene            719..1300
                     /gene="bfr"
                     /locus_tag="VGC27_10815"
     CDS             719..1300
                     /gene="bfr"
                     /locus_tag="VGC27_10815"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY0283101.1"
                     /translation="MPRNNRGNVGKDAGKRVSGQGGVGPAPKASLPGDPQVIGFLNTA
                     LKNELTAISQYFLHSRMLADWGISELAKKEREESIDEMKHADRLIQRILFLGGLPNLQ
                     NIGKLQIGENLKEVISCDLALEQHALPDLQSAIVHCERIGDYVSRELFVDILKSEEDH
                     IDFLQTQFRLVEQMGIENYAQLQTKPNAAGAAS"
     gene            complement(1352..3649)
                     /gene="metE"
                     /locus_tag="VGC27_10820"
     CDS             complement(1352..3649)
                     /gene="metE"
                     /locus_tag="VGC27_10820"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY0283102.1"
                     /translation="MSIPVSTLGFPRIGPRRELKKALESYWSGQSSAEALLREGAALR
                     AAAWARQQAAGISVIPSNDFSLYDHVLDASVMVGAVPGIYERASGPVTLDTYFAMARG
                     THSRAGGDLPALEMTKWFDTNYHYMVPEFWRGQSFELRSTKPIDEFREAKSLGIKTRP
                     VLLGPVTYLLLGKSKDESFDPLLLLPALLPVYVEVLNHLAAAGADWVQMDEPCLVQNL
                     SDHAKTALRETYRTLAAAVPGLKLLLTTYFGGLGENLETALSLPVAGIHLDLVRAPGQ
                     LDEILHKAPGDLVLSLGVIDGRNVWRADLAAILDRLEPVVSAWRPERLMLGPSCSLIH
                     TPLDLDLETGLDPDLKSWLAFAVQKLEELSTLGRALDEGRGAVADVLAASVRAAEARK
                     SSSQIHDTAVERRLAAVTPDMTQRASDFRLRRTIQRQHMVLPSFPTTTIGSFPQTKEV
                     REARASHARGDLDDAAYENFLRRETAACIKWQEEVGLDVLVHGEFERNDMVQYFGEQL
                     SGFAFTKHGWVQSYGSRYVRPPIIFGDVSRPRPMTVNWAAYAQSLTKRPIKGMLTGPV
                     TMLQWSFVRDDQPRERTCRQIALALRDEVLDLEKAGIRVIQIDEPAIREGLPLRKQDW
                     KAYLDWAVECFRLASSGVWAGTQIHTHMCYSEFNDIIESVGAMDADVISIETARSKME
                     LLYAFATYRYPNEIGPGVYDIHSPRCPSVEEIVALLAKAKERISPDHIWVNPDCGLKT
                     RRWEEVKPALLNMVEAARRLRADFA"
     regulatory      complement(3844..4052)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     regulatory      4214..>4390
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          908 a         1309 c         1299 g          874 t
ORIGIN      
        1 gacggtcttc tacgacaagg acggcatcga gctccgcgtg gccgtcaact ggcgcgacac
       61 gtacctcgac aggttcggtc acggccagaa tggcggtacg agtttcggtt cggaaccgat
      121 cttcgtgaac ggcaactggg atctgaccgt cggcggcggc taccagatca cggacaacat
      181 caaagcctac ttcacggcga acaacctgct ggacacggtc tattccacgc gcggccgctt
      241 tccggatcag gtgtacggcg tgatcaccat cggccgcagc tttacggccg gcgtgcacta
      301 caggatgtag tctcccccgc taactgaaag ggccgccgac atccgtcggc ggcctttttt
      361 tgtatttcgc gaactcgacc ggcgcaggca gcaaaaagct gatcggcctc gtcacgccga
      421 gagtaatgtc atcaggagta taataatcat cagcgccgga gccggcgtta gcgtggaagc
      481 tattgcgatt cttacggttt ttgtgttcta cacccctgct gacccagccg ccagtatgtc
      541 gcatttgata atcattctca tataatctat gtattattgg tacccaattg gtcccgtgga
      601 tgatcgtctg cgtttgcaat aggttgaatg agagccgggt tcgggccgcc atcacggctg
      661 gcgccaagac gccggacgat gtttattcaa cctgtggcgt gacgaggaat tgcggacgat
      721 gccaagaaac aatcgtggaa atgttgggaa agacgcagga aagcgcgtct ctggtcaagg
      781 cggcgtaggc cccgcgccca aagcttcgct gccgggcgac ccgcaggtca tcggttttct
      841 caacaccgcg ctcaagaacg aactgactgc gatcagccag tacttcctgc attcgcgcat
      901 gctggcggac tggggcatct ccgaactcgc caagaaagaa cgggaagaat ccatcgacga
      961 gatgaagcac gccgatcgcc tgatccagcg cattcttttt ctcggcggcc tgcccaatct
     1021 tcagaacatc ggcaaattgc agattggcga gaacctcaaa gaagtcattt cctgcgatct
     1081 cgcgcttgag caacacgccc tgcccgatct gcagtccgcc atcgtccatt gcgaacggat
     1141 cggcgattac gtatcgcgcg agttgtttgt ggatattctc aagagcgagg aagatcatat
     1201 cgacttcctc cagacccagt tccggctggt cgagcagatg ggcatcgaga attacgcgca
     1261 gctccagacc aagcccaacg ccgcgggcgc ggcgtcctga agctctggca cgtctcatcg
     1321 cggccgatcg gacttccgag gccgcgacat cctacgcaaa gtcggctcgg agccgcctcg
     1381 cggcttcgac catgttcagc agggccggct tgacctcctc ccaacgcctg gtcttcaacc
     1441 cgcaatcggg atttacccag atgtgatccg gcgaaattct ctccttcgcc tttgcgagca
     1501 gagcgacgat ttcctcgacc gaaggacagc gcggcgaatg aatatcgtaa acgccggggc
     1561 cgatctcatt cggatagcga taggttgcga aggcgtatag cagctccatt ttcgagcgcg
     1621 ccgtttcgat ggaaatcacg tcggcgtcca tggcaccgac ggattcgatg atgtcgttga
     1681 attccgaata gcacatatgc gtgtggattt gcgttcccgc ccacacgccc gacgaggcca
     1741 agcggaagca ttccaccgcc caatcgagat aggccttcca atcctgtttg cggagcggca
     1801 gcccctcgcg gatggccggc tcgtcgattt ggatgacgcg gattccggcc ttttccagat
     1861 ccaggacttc gtcccttagg gccagcgcga tctgccggca ggtccgttcg cgcggctgat
     1921 cgtcccgtac aaaggaccat tggagcatcg tgacaggccc cgtcagcatg ccttttatgg
     1981 ggcgtttggt gagggattga gcgtaagcgg cccagttgac cgtcatcggc cgcggacggc
     2041 tgacatcgcc gaaaatgatg ggggggcgga catagcgcga accatagctc tgcacccagc
     2101 catgcttggt gaaggcgaaa ccggaaagct gttcgccgaa atactgtacc atgtcgttac
     2161 gctcgaactc gccatggacg agcacgtcaa ggccgacctc ctcctgccat ttgatgcagg
     2221 ccgccgtctc ccggcgcaaa aaattctcat aggccgcgtc gtccaggtcg ccccgcgcat
     2281 gagaggcgcg cgcttcgcgg acttctttcg tttgaggaaa cgatccgatg gttgtggttg
     2341 ggaatgacgg caaaaccata tgctgccgct ggatcgtgcg gcgcaaccgg aaatcggacg
     2401 ctcgttgcgt catgtccggc gtgacggcgg ccaggcgcct ttcgacggcg gtatcgtgaa
     2461 tctgcgagga ggacttgcgc gcttccgcgg cgcgaacgga agcggccagc acgtccgcca
     2521 ccgcgccgcg cccctcgtcc agcgcccggc caagcgtgga gagttcttcg agcttctgca
     2581 ccgcaaaagc aagccagctt ttgagatcgg gatccagacc ggtttccaaa tcgaggtcga
     2641 gcggcgtgtg gatcagcgag catgatggac cgagcatcaa gcgttccggg cgccacgccg
     2701 acaccacagg ttccaggcgg tcaaggatcg ccgccagatc agcgcgccag acgttcctgc
     2761 cgtcgatgac cccgagcgac aacacgagat cgcccggcgc cttatgcaga atctcgtcca
     2821 gctggcctgg cgcccgcacc agatcgagat ggattccggc gacgggaagc gacaaggctg
     2881 tttcaagatt ctcgcccagc cccccgaaat acgtcgtcaa caggagtttc agccccggca
     2941 cggccgcagc gagcgtccga taagtttcgc gcaaggcggt tttggcgtgg tcgctcaggt
     3001 tctgaaccag acagggttcg tccatttgaa cccagtctgc gccggccgcg gccaaatggt
     3061 tcagcacctc cacgtagacc gggagcaacg caggcaacag caggagggga tcgaagcttt
     3121 cgtctttgct cttgccgagc aaaagatagg tcacaggacc gagcagaacc gggcgcgttt
     3181 tgatgccgag ggactttgct tcacggaact cgtcgatcgg ttttgtgctg cgcaattcga
     3241 aactctggcc gcgccagaac tccggcacca tgtagtggta attggtgtcg aaccacttgg
     3301 tcatttccag cgccggaaga tcgccgccgg cgcggctgtg cgtaccgcgc gccatcgcaa
     3361 aataggtgtc gagagttacc gggccggagg cacgctcgta aatgcctggg acggcgccaa
     3421 ccatgacgct ggcgtctagc acatggtcat agagcgaaaa gtcgttgctc ggaatcacgc
     3481 tgattccggc agcctgctgg cgggcccaag cggccgcacg gagggccgcg ccttcgcgca
     3541 gcaacgcttc ggcactgctc tggccggacc aatagctttc aagggctttt ttcagttcgc
     3601 ggcgcggacc gatgcgtgga aaacccagcg tgctgacggg gatggacata gagcggcctt
     3661 tcgcgtttgc cctagctcag aagagcatcg aggcttggaa aaatccgccc acgggcattg
     3721 tactgattgc ggatggtaaa atgacatcgg catacccttt gggggcatgg actaagcgga
     3781 acgcatgcgg atgtcagacg ggatatccgc ctgctgcaag cgacccaccg gagcaccccg
     3841 cccgtggacg ttctctgtcg cggcaggtct cctggcttgc gggttgttgc ttccgtcagg
     3901 ccttcccgat gcgccgcatc agtggcatgg acgacggaaa ctcgccgctt acagttgcgg
     3961 gggcagcttc ggcattgcct gacggcgcac cgaattccct cttagcttcc ggccaaatag
     4021 cggacggaag aaccacgaca agcctcatta agcggaagct gcatggggct gtcaataggg
     4081 atataaagct ttctttatat gttttccagc ttcctttcca agggatcgct gggtattgtg
     4141 aacgtccagc agcgcagaaa atatttttac cgcacatgca tgatttccgg cttgacgcta
     4201 ccagctcatt gccacacgat ctagccgtca cggttcttcg gatacacaat atctggagct
     4261 aagtgggaat ccggtacggc tgataccgct aaatgaacgg cccgatccgg agctgccccc
     4321 gcaactgtaa gcggcgagtt tccgtcgtcc atgccactga tgcggcgcat cgggaaggcc
     4381 tgacggaagc
//
LOCUS       DASWPU010000511         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_89962,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000511 DASWPU010000000
VERSION     DASWPU010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89962"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VGC27_10825"
     CDS             complement(<1..733)
                     /locus_tag="VGC27_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0283103.1"
                     /translation="MRLMWLLFAFSGPVLWAASTHIDKYLVDRYFKDSDTAVLIVFTA
                     LIGIAMLPFIWFFRPGVLALSGTNIAVMVASGVLFMGAMLFYLRAIQSEEASVVAPLF
                     QTSVLFTFLLAYLLLGETLTASKAAGLVLIVAATLVLSFDPSLSLRRFKLRLVLLMLA
                     CTFTLALASVIFKFYAVQDEFWATTFWMFAGEALYGVVFLSIPSYRAQFIHLLRSNGK
                     ALLAINASNELINLGGALANRYALIF"
     gene            complement(767..1840)
                     /locus_tag="VGC27_10830"
     CDS             complement(767..1840)
                     /locus_tag="VGC27_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEY0283104.1"
                     /translation="MLTRRSVLGGVAAGVLIPPGRAAAAEAPLRQRAAKRGLYYGCAA
                     GSYQLKDADFAQVLAREANMLVPEYELKRLIVEPQPGQFDYSGTDALMAFAGAHGMKM
                     RGHTLVWYAANPPWLEDKVLGAPDDEILTDYVARLMGRTRGRIHSWDVVNEAIHPEDG
                     RPDGLRDCFWLKRFGPAYIDLAYRAAKQADPAALLVYNDWGCEGGEAWNDRFRAATLK
                     FLSDALARKVPIEALGLQGHVNAFGPGVDQKKLRVFLDEVRAMGLRLLVTEHDVDDSG
                     AAPDIAARDRAVADASARFLDVVLDNDATIAVLTWGLTDRYLKAPGGLRAAFSGSSPR
                     KLPLDAELKRKPMWGAIAKAFGG"
     gene            complement(1850..2275)
                     /locus_tag="VGC27_10835"
     CDS             complement(1850..2275)
                     /locus_tag="VGC27_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0283105.1"
                     /translation="MIILDTNVVSEMMKRDKADHAVTAWLDRQRGDTVWLTSISVFEV
                     TFGVELLARGQKRSRLEAEFARAVREDFQERILAFDREAAEAAAGFSARRRASGRPVE
                     FRDAQIAGIVAANDATLATRNIRDFQDFAIELANPWTDE"
     gene            complement(2272..2523)
                     /locus_tag="VGC27_10840"
     CDS             complement(2272..2523)
                     /locus_tag="VGC27_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283106.1"
                     /translation="MAQLVVRNLEERVKERLRRQARRNGRSMEEEARDILRKAFPSRP
                     VPPEEGLGTRLRRRFEGSGIDFEIPEFRGYEARPAKFEK"
     gene            complement(2578..>2982)
                     /locus_tag="VGC27_10845"
     CDS             complement(2578..>2982)
                     /locus_tag="VGC27_10845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0283107.1"
                     /translation="IKELTRAGFYDGVVFHRVIPGFMAQTGDPTGSGSGGSDLPDLEA
                     EFSGARHVRGTVSMARTSDPHSANSQFFICFDEAPWLDRQYTVWGQVVEGMEHVDAIK
                     KGGEHNNGAVSGAPDKIVKLRIEADGSENQAR"
BASE COUNT          569 a          983 c          912 g          518 t
ORIGIN      
        1 cgaagatcaa ggcgtagcga ttggccaagg cgccgccgag attgatcagc tcattggatg
       61 cattgatcgc gagcagcgcc ttgccgttgc tgcgcaagag atggatgaac tgcgcgcggt
      121 agcttgggat gctgaggaag accacgccat acagtgcctc gccggcgaac atccagaacg
      181 tcgtcgccca gaactcgtcc tgcaccgcat agaatttgaa gatgaccgac gccagcgcca
      241 gggtgaaggt gcaagccagc atgagaagga cgaggcgaag cttgaaccgg cgcaggctca
      301 gggacgggtc gaacgaaagg acgagcgtgg ccgcgacgat gagaacgagg cccgcggcct
      361 tgctcgccgt cagcgtttcg ccgagcagaa gataggccag cagaaaggtg aagagcaccg
      421 aggtctggaa cagcggcgcg acgaccgagg cctcttccga ctggatggcg cgcaagtaga
      481 acagcatcgc gcccatgaac aggacgccgg aagcgaccat cacggcgatg ttcgttcccg
      541 acagcgccag cacgccgggc ctgaagaacc agatgaacgg cagcatcgcg atgccgatca
      601 gcgccgtgaa cacgatcaag accgccgtgt cgctgtcctt gaaatagcgg tcgacgaggt
      661 acttgtcgat gtgcgtggag gcggcccaca gcaccggccc ggaaaacgca aacagcagcc
      721 acatgagccg catattgcat tgcgcctccg ccgagtaaac ggcctgctag ccgccgaatg
      781 cctttgcgat ggcgccccac atcggcttgc gcttgagctc cgcgtccaag ggcagcttcc
      841 tcggcgagga tcccgaaaac gccgcccgca gcccgccggg ggctttcaga tagcggtccg
      901 tcagccccca ggtcagcacc gcgattgtag cgtcgttgtc cagcaccaca tccagaaagc
      961 gcgcgctggc gtcggcgacc gcccggtcgc gcgccgcgat gtccggcgct gcgccgctgt
     1021 cgtccacgtc gtgctcggtc accaggaggc gcagccccat cgcccgtacc tcgtcgagga
     1081 aaacgcgcag cttcttctga tcgacgccgg ggccgaacgc attcacatgc ccttgcagcc
     1141 ccagcgcctc gatcggcacc ttgcgcgcca aagcgtcgga gaggaatttc agggtcgcgg
     1201 cgcggaagcg gtcgttccag gcctcgccgc cttcgcagcc ccagtcgttg tagaccagca
     1261 gcgcagcggg atcggcctgt ttggccgccc gataggcaag gtcgatataa gccggcccga
     1321 agcgcttgag ccaaaaacaa tcgcgcaatc catccgggcg gccgtcttcg ggatggatcg
     1381 cctcattgac gacatcccag gaatggatgc ggccgcgagt gcgccccatg agccgcgcaa
     1441 cataatccgt cagaatttcg tcgtccggcg cgcccagcac cttgtcttca agccagggcg
     1501 ggttggcggc gtaccagacc agcgtgtggc cgcgcatctt catgccgtgc gcgcccgcga
     1561 acgccatcag agcgtccgtt cccgaatagt cgaactgtcc cggctgcggt tcgacgatca
     1621 ggcgcttcaa ttcgtattcg ggcacgagca tgttggcttc gcgcgccagg acttgcgcga
     1681 aatccgcatc cttcaattgg taggaccccg cggcgcagcc gtaatacaat ccgcgcttcg
     1741 ccgcgcgctg gcgcaagggc gcctcggcgg cggcggcccg gccgggcggt atcagcactc
     1801 ccgccgcaac gcctcccaga accgatcgtc tcgtcagcat gaaggcacct cactcatccg
     1861 tccacggatt ggccaactct atcgcgaaat cttgaaaatc ccttatattg cgagtcgcca
     1921 gcgtggcatc attcgcggcg acgattccgg cgatctgggc atcgcggaac tcgacggggc
     1981 ggccggacgc ccggcggcgc gcggagaaac cggctgccgc ctctgccgcc tcgcgatcga
     2041 atgccaaaat tctctcctgg aaatcttctc tcacggcgcg cgcgaattcc gcttcgagcc
     2101 ggctgcgctt ctgtccccgc gccaacaact ccacgccgaa ggtaacctcg aaaacactga
     2161 tgctggtcaa ccagacggta tcgccgcgtt ggcggtccaa ccatgcggta acggcatgat
     2221 cggccttgtc gcgtttcatc atttcagaga caacgttcgt gtcgagaata atcatttctc
     2281 gaacttggcg ggacgcgctt cgtatccgcg gaattccggg atttcgaagt caatgccgct
     2341 tccctcaaac cgccggcgca agcgcgtccc gagaccctcc tcgggaggca cgggtcttga
     2401 cggaaatgcc ttgcgcaaga tatcccgcgc ctcttcttcc atgctgcgcc cgttccggcg
     2461 cgcctggcgg cgcaagcgct ctttcacacg ctcttcgaga ttgcggacga caagctgcgc
     2521 catatattcc gttctccagt caatgtgata tcatgatatc actggagtcc atggccgtca
     2581 acgagcctga ttttcgctcc cgtccgcctc gatccgcagc ttcacgatct tgtcgggcgc
     2641 gccggagacg gcgccgttat tgtgctcgcc gcccttcttg atggcgtcga catgttccat
     2701 gccttcgacc acctggcccc aaaccgtgta ttggcggtcg agccaaggcg cctcgtcgaa
     2761 gcagatgaag aactggctgt tggcgctgtg cgggtcggag gtgcgcgcca tggagacggt
     2821 tccgcgcacg tgccgggcgc cggaaaattc cgcctcgaga tcgggcaggt ccgagccgcc
     2881 gctgccgctg cccgtgggat cgccggtctg cgccatgaag ccgggaatga cgcggtggaa
     2941 cacgacgccg tcgtagaacc ctgccctggt cagttccttg at
//
LOCUS       DASWPU010000512         6736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_121067,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000512 DASWPU010000000
VERSION     DASWPU010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121067"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..232
                     /locus_tag="VGC27_10850"
     CDS             2..232
                     /locus_tag="VGC27_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0283108.1"
                     /translation="MKLNLDRVMLERHVSLTELAERIGITLANLSILKTNKARAIRFS
                     TLEALCRELQCQPGELLEFVPHGEAAESQDEV"
     gene            418..807
                     /locus_tag="VGC27_10855"
     CDS             418..807
                     /locus_tag="VGC27_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0283109.1"
                     /translation="MGAVRIFKTKWLARYARRESITDASLREAVSRAIRGSVDADLGG
                     NVIKQRVARTGRGRSGGYRMLIAFRAAYRAVFMLGFAKNDRDNITADELESLKKIAAL
                     WLSADAAKIEQVIAEGILVEVKHENEK"
     gene            791..1129
                     /locus_tag="VGC27_10860"
     CDS             791..1129
                     /locus_tag="VGC27_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0283110.1"
                     /translation="MKTKNKKPGPVTKAILEMADGMHSVGIMNDADYNKITMRHLGKE
                     PVAAVRPLTPDDIRSLRAGARMSQAVFARHLNVTTGYVSQLERGAKHPTGAALVLLNV
                     IRRKGIEAIL"
     gene            complement(1215..2201)
                     /locus_tag="VGC27_10865"
     CDS             complement(1215..2201)
                     /locus_tag="VGC27_10865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0283111.1"
                     /translation="MKSHAFGRLPFTVGNIGFGAWQIGGGWGDVSEADGRAALNAALD
                     AGMSFIDTADVYGDGRSEKIIAEVLKARGGARPMVATKAGRRLNPHTADGYNKANLEA
                     FIDRSLKNLAADSLDLVQLHCPPTEALYRPELFAALDDIRKSGKIKSYGVSVEKVEEA
                     LKAIEFPGVVSIQIIYNLFRQRPAELFFKEAKRKNVAVIVRVPLASGLLTGKITCDTK
                     FAAGDHRNFNRRGEAFDVGETFAGVPLETGLEAVEALRKLVPQGASLAAFALRWILMN
                     NAVTVVIPGARNAEQAKANAAASDLAPLSAETMAAARGIYDRLIAPHVHQRW"
     gene            2393..3067
                     /locus_tag="VGC27_10870"
     CDS             2393..3067
                     /locus_tag="VGC27_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0283112.1"
                     /translation="MKIGIIGAGKIGGTLTRRFRALGHEVFIANSRGPQTLAELAKET
                     GAKAVTAAEAARGGGVVVVTIPMKNIPQLPAGLFDGVPEDVVVIDTGNYYPQQRDGRI
                     DEIEKGLLESRWVERQIGRKVVKVFNNILAKHLLELGKPKGAPGRIALPVAGDDAREK
                     AVVLALVEELGFDAIDAGGLDESWRQQPATPVYGTDLDAEGVRRALAAASPERQPAWR
                     AGATSP"
     gene            3241..3846
                     /locus_tag="VGC27_10875"
     CDS             3241..3846
                     /locus_tag="VGC27_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY0283113.1"
                     /translation="MQTLVPMVVEQTARGERAYDIYSRLLKERIIFVTGPVEDYGASL
                     VTAQLLFLEAENPKKEISMYINSPGGIVTAGMAIYDTMQYIRPSIQTLCVGQAASAAS
                     LLLCAGTKGQRFCLPNSRILVHQPSASYYGQAADIARHAQEIVKLKRRLNEIYAEHTG
                     QSVEAIEKALDRDTYMNAEEAKAFGLVDSVLSRRPEAVENS"
     gene            4034..5299
                     /gene="clpX"
                     /locus_tag="VGC27_10880"
     CDS             4034..5299
                     /gene="clpX"
                     /locus_tag="VGC27_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY0283114.1"
                     /translation="MSKQNGGESKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MEIIREENKTSFMKSREGVPSPAEIFKVLDDYVVGQAHAKKVLSVAVHNHYKRINAGG
                     KNSEVELAKSNIMLLGPTGCGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENI
                     ILKLLQNADYNVERAQRGIVYIDEVDKISRKSDNPSITRDVSGEGVQQALLKIMEGTI
                     ASVPPQGGRKHPQQEFLQVDTTNILFIVGGAFAGLEKIISQRTHGSSMGFGANVRAPD
                     ERATGEVLREVEPEDLLKFGLIPEFIGRLPVLATLGDLSETALIEILTRPKNALAKQY
                     QRMLEMEGVKLRFQEEALHAIAKRAIARKTGARGLRSIMEGILLDTMYELPTLNGVQE
                     VVITADVVDGRARPLYTYSDKGQAQQSAS"
     gene            5342..5779
                     /locus_tag="VGC27_10885"
     CDS             5342..5779
                     /locus_tag="VGC27_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HEY0283115.1"
                     /translation="MVQAPPRMIDRNGPPGAAELASFLGKRAYGYWRRITGRIERNYP
                     GVFSPDWLFSKNQGWMLRYKKNKSFCTLIPERKRLRVQIVLGAGEREKVEARRGELSA
                     PTRKTYDEATVYHDGKWLYLTVDAKETLEDVVQLLAVKRKPAV"
     gene            6038..>6736
                     /locus_tag="VGC27_10890"
     CDS             6038..>6736
                     /locus_tag="VGC27_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY0283116.1"
                     /translation="MSDQEVVTTGRGEPGASMAPVLPLRDIVVFPHMIVPLFVGREKS
                     VRALDEVMNEDKQILLLTQKNAADDDPASDGLHRIGTLATVLQLLKLPDQTVRVLVEG
                     KSRARVTGFVPRTDFFQATIEPVPDSVGEAKETEALIRTVKTNFEQYIKLNKKVPAET
                     LASVAQIDEPAKLADTVASHLSVKIADRQALLETLNTAERLEKVLSLIESEIGVLQVE
                     RKIKSRVKRQMEKTQ"
BASE COUNT         1362 a         2057 c         2138 g         1179 t
ORIGIN      
        1 catcaagctc aacctcgacc gcgtgatgct cgaacggcac gtcagcctga cggaactggc
       61 ggagcgtatc ggcatcacgc tggccaatct ctcgatcctc aagaccaaca aggcacgcgc
      121 catccgcttc agcacgctgg aagccttgtg ccgcgaactc caatgccagc cgggcgaact
      181 gctggagttc gttccgcacg gggaggccgc ggaaagtcaa gacgaagttt aaagcggata
      241 accgctgggt gctcgacttc gcgaacatga acttctcgct caccgactga acgccagcgg
      301 cgcggtgccg ttcgcgggtc ccggcgaagc gcgaagaaga taaaggctga ataaggcagt
      361 tcggctcaac caattacttg aaattatagt tcatggtgct atagttcata gtgctagatg
      421 ggcgccgtga ggatattcaa gaccaaatgg cttgcgcgat atgcgcgacg ggaaagcatc
      481 accgatgcga gcctgcggga agctgtctcg cgcgcaatac gcggctcggt cgatgccgat
      541 ctgggcggca acgtcatcaa gcagcgggtg gcgcgaacgg ggcggggccg ctccggcggc
      601 tatcgcatgt tgatcgcttt ccgggcggcc taccgcgcgg tgtttatgct tggctttgcc
      661 aagaacgacc gggacaacat cacggcggat gaattggaat cgctcaagaa gatagcggcg
      721 ctgtggctct cggccgatgc ggcgaaaatc gaacaagtca ttgccgaagg tattttggtg
      781 gaggtgaaac atgaaaacga aaaataagaa gcccggcccc gtgacgaaag ccattttgga
      841 aatggcggac ggcatgcata gcgtcggcat tatgaacgat gccgattaca acaagatcac
      901 gatgcggcac ctcggcaagg aacccgtggc agcggtccgg ccgctgacgc cggacgacat
      961 tcgctcgctg cgtgcgggcg cgcggatgag ccaggccgtt ttcgcgcgcc acctcaacgt
     1021 gacgaccggc tatgtgtcgc aattggagcg gggcgccaaa catccgaccg gtgcggctct
     1081 cgtgctgctg aacgtcatcc gccgcaaggg gattgaagca attctgtaag acggagttcc
     1141 tcgatcccga cgaggcggcg gagaagatga cggaaacctg agcgcacgga tcggagactg
     1201 gcgtcccgca tgcctcacca ccgctgatgg acgtgcggcg cgatcagccg gtcgtaaatc
     1261 ccgcgcgccg ccgccatcgt ctcggcggac agcggcgcca gatcggacgc cgccgcgttg
     1321 gccttggcct gttcggcgtt gcgggcgccg gggatgacca cggtgacggc attgttcatc
     1381 aagatccagc gcagcgcgaa ggcggccagg ctcgcgccct gcggcaccag cttgcgcaag
     1441 gcctcgacgg cctcaaggcc ggtctccagc gggacgccgg caaaagtctc gccgacatcg
     1501 aacgcctcgc cgcggcggtt gaaattgcgg tggtcgccgg ccgcgaattt ggtgtcgcag
     1561 gtgatcttgc cggtaagcag gccgctcgcc agcggcacgc ggacgatcac ggcaacgttc
     1621 ttgcgcttgg cttccttgaa aaacagctcg gcagggcgct ggcggaacag gttgtagatg
     1681 atctgtatgc tgacgacgcc ggggaattcg atcgctttca gcgcttcctc gaccttttcg
     1741 acgctgacgc cgtagctttt gatcttgccg gacttgcgga tgtcgtcgag cgccgcgaac
     1801 agttcggggc gatacagcgc ctcggtgggc gggcagtgaa gctggacgag gtcgaggctg
     1861 tcggccgcca gattcttcag gctgcggtcg atgaaggctt cgagattggc tttgttatac
     1921 ccgtcggcgg tatgcggatt gagccgccgc ccggccttgg tcgccaccat cggacgggcg
     1981 ccgccgcgcg ccttcagcac ctcggcgatg atcttttccg agcggccgtc gccgtaaacg
     2041 tcggcggtat cgatgaagct catgccggca tcgagcgccg cgttcagcgc cgcgcgcccg
     2101 tcggcttcgc tgacgtcgcc ccagcccccg ccgatctgcc aggcgccgaa gccgatattg
     2161 ccgacggtaa acggcagacg gccgaaagca tggctcttca tgaagattct ccattcgatt
     2221 tgcggacaat gcgggcttaa caattggaag acgcacggac aagtccggac gaaacccata
     2281 ccgtttatgt agtatcgcct gttccggaat tccccgttcg tggttttccg ttgccgttcg
     2341 cccgcgcgcg ttttaatctg aaaatgccgt ctgtcgttga aagggtggca tcatgaaaat
     2401 cggcatcatc ggagcaggca agatcggcgg tactttgacg cgccgcttcc gggcgcttgg
     2461 gcacgaggtt ttcatcgcca attcgcgagg tccccagacg ctcgcggagt tggcgaagga
     2521 aaccggcgcc aaggcggtca ccgccgccga ggcggcgcgc ggtggcggcg tggtcgtcgt
     2581 caccatcccg atgaaaaaca tcccgcagct tcccgccggc ctgttcgacg gcgtgccgga
     2641 ggatgtcgtc gtgatcgaca ccggaaacta ttatccgcaa cagcgcgacg gccgtatcga
     2701 tgagatcgaa aagggcctgc tcgaaagccg ctgggtggaa cggcagatcg ggcgcaaagt
     2761 ggtcaaggtg ttcaataaca tccttgccaa gcatctgctg gaattgggca agcccaaagg
     2821 cgcgccgggg cgcatcgcgc tgccggtggc gggcgacgac gcgcgggaga aagccgtggt
     2881 gctggctctg gtcgaggaac tcggctttga cgccatcgat gcgggcggcc tcgacgaatc
     2941 ctggcgtcaa cagcccgcaa ccccagttta cggcaccgat ctggacgccg aaggcgtgcg
     3001 ccgcgctctg gccgcggcca gccccgagcg ccagccggcg tggcgcgccg gcgcgaccag
     3061 cccctgaaat ttcgaggcgc ctgcctggtc ttcctgcttc tgccatagtc cggcgcgggg
     3121 ccgtttaccc gccgacaagg ggcgaaagct actatcggcg ttgacacggc gcttcggcgc
     3181 gctccacaac cctgtcgacc gaattcagga gggcccatgc aggacccacg cgacgtttac
     3241 atgcagacgc tggtgccgat ggtggtcgag cagaccgccc gcggcgagcg cgcctacgac
     3301 atctattccc ggctgctcaa ggagcggatc atcttcgtca ccggcccggt ggaggattac
     3361 ggcgccagcc tggtcaccgc ccagcttctc ttcctggagg ccgaaaaccc caagaaagag
     3421 atttcgatgt acatcaactc gccgggcggc atcgtcacgg cgggcatggc gatctacgac
     3481 acgatgcagt atatccgccc ctccatccag acgctgtgcg tcgggcaggc ggcgtcggcc
     3541 gcgtcgctgc tgttgtgcgc ggggacgaaa ggtcagcgtt tctgccttcc caattcgcgc
     3601 attctggtcc accagccgtc ggcgtcctat tacgggcagg cagccgacat cgcccgccac
     3661 gcccaggaaa tcgtcaagct taagcggcgt ttgaacgaga tttacgcaga gcatacaggg
     3721 cagtccgtgg aggccattga aaaggcgctg gatcgcgata cttatatgaa tgccgaagag
     3781 gcaaaagcct tcgggttggt ggattccgtg ctatccaggc gcccggaagc ggtcgagaat
     3841 tcttaacgat acgaaatccg gccgatatat cagtatgttg tacggcttaa acaaatcttg
     3901 atccgcccgg cgttaacgtg atcgaatgaa atcggcggca agccgatttg ggttggcgcg
     3961 ggttgtgcag ggctttgatt gcaatgcccg aaacggtctt taagggcggg tcgcaaacga
     4021 cccgggagag ccgatgagta aacagaacgg cggcgagtcc aagaacacgc tttactgttc
     4081 cttctgcggg aaaagccagc acgaggttcg caagctgatc gcgggcccga cggtgttcat
     4141 ctgcgacgaa tgcgtcgagc tgtgcatgga gatcatccgc gaggagaaca agacctcctt
     4201 catgaagtcg cgcgaaggcg tgccttcgcc ggcggaaatt ttcaaggtgc tggacgatta
     4261 cgtcgtcggc caggcgcacg ccaagaaggt gctctcggtc gcggtccaca accactacaa
     4321 gcgcatcaac gccggcggca agaattccga agtcgaactg gcgaaatcga acatcatgct
     4381 gctcggcccg acgggttgcg gcaagacgct gctggcccag acgctggcgc gcatcctcga
     4441 cgtgcccttc accatggccg acgccaccac cctgaccgaa gccggttacg tcggcgagga
     4501 cgtggagaac atcatcctca agctgttgca gaacgccgat tacaacgtcg agcgggcgca
     4561 gcgcggcatc gtctatatcg atgaagtcga caagatcagc cgcaagtccg acaacccctc
     4621 gatcacccgc gacgtctcgg gcgaaggcgt gcagcaggcc ctcctcaaga tcatggaagg
     4681 caccatcgcc tccgtgccgc cgcagggcgg gcgcaagcat ccgcagcagg aattcctgca
     4741 agtggacacg accaacatcc tcttcatcgt cggcggcgcc tttgccggcc tggagaagat
     4801 catctcccag cgcacccacg ggtcctcgat gggtttcggc gccaatgtcc gcgcccccga
     4861 cgagcgcgcc accggcgaag tgctgcgcga ggtcgaaccc gaagatctgc tcaaattcgg
     4921 gctgattccg gaattcatcg gccgtctgcc ggtgctggcg acgctcggcg acctgtcgga
     4981 aaccgctctg atcgaaatcc tgacgcggcc caagaacgct ctggccaagc agtatcagcg
     5041 catgctcgag atggagggcg tcaagctgcg cttccaggaa gaggcgctgc acgccatcgc
     5101 caagcgggcc attgcccgca aaacaggcgc ccgcggcttg cgctcgatca tggaaggcat
     5161 cctgctggac accatgtacg agctgccgac gctcaacggc gtgcaggaag tcgtcatcac
     5221 cgccgatgtg gtggacggcc gcgcccggcc gctttacacc tattccgaca agggccaggc
     5281 gcagcaaagc gcgtcttgac cggttttatg tgacctgaat ttcttgctac gcttggccgt
     5341 catggttcag gccccgccac gcatgatcga ccggaacgga ccgcccggcg ccgcggaact
     5401 cgcgtctttt ctcggcaaaa gggcctacgg ttattggcgg cgcatcaccg ggaggatcga
     5461 gcgcaactat cccggcgtgt tttcgcccga ctggctgttc agcaagaacc agggatggat
     5521 gctgcgctac aagaaaaaca aatccttctg cacgctcatt ccggagcgga aacgcctgag
     5581 ggtgcagatc gttcttggcg ccggcgagcg ggaaaaagtc gaagcccgcc gcggcgaatt
     5641 atcggcccct acgcgcaaaa cctacgacga agcgaccgtc tatcacgacg gaaaatggct
     5701 gtatctaaca gtggatgcga aggagaccct cgaggacgtc gtgcagcttc ttgcggtgaa
     5761 gcgcaagccg gctgtttgag ggcgacggcc gctcgtccgg caagggtcga gcgcggcaaa
     5821 gcgccccttt acggcagctt ttctgtggct gcgatggcgc ggcgggagga ttaaagccct
     5881 gtaaggagct tgccgccttg aaacagccac gggccgggcc cactttacat tatctaggag
     5941 tcggcccatc cgggcgacaa atgaggttcc ctacgcccat caaggggtac ggttcctaaa
     6001 cgcactccgt ccatgccggc ggagcgagga gaaggtaatg tcggatcaag aggtcgtgac
     6061 aaccggaaga ggcgagccgg gtgcgtccat ggcgccggtg ctgcccctgc gcgacatcgt
     6121 cgtgtttccc catatgatcg ttcccttgtt cgttggccgc gagaagtcgg tgcgcgcgct
     6181 cgacgaggtg atgaacgaag acaagcagat tctgctgctc acccagaaaa acgccgccga
     6241 cgacgatccc gcttccgacg gcctgcaccg gatcggcacc ctggccaccg tgcttcagct
     6301 cttgaagctg ccggaccaga ccgtgcgcgt gctggtggag ggcaagagcc gcgcccgcgt
     6361 caccggtttc gtgccgcgaa ccgatttctt ccaggcgacg atcgaacccg tccccgacag
     6421 cgtcggcgag gcgaaggaga ccgaagcgct gatccgcacg gtcaagacca atttcgagca
     6481 gtacatcaag ctcaacaaga aggtgccggc cgagaccctg gcctcggtgg cgcagatcga
     6541 cgaaccggcc aagctggccg acacggtggc ctcgcatctt tccgtcaaga tcgccgaccg
     6601 ccaggcgctg ctggaaacgc tgaacacggc cgaacggctg gagaaggtgc tgtcgctgat
     6661 cgaatccgag atcggcgtgt tgcaggtcga gcgcaagatc aagagccgcg tcaagcgcca
     6721 gatggagaag acacag
//
LOCUS       DASWPU010000513         4853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_66204,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000513 DASWPU010000000
VERSION     DASWPU010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66204"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VGC27_10895"
     CDS             <1..272
                     /locus_tag="VGC27_10895"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY0283117.1"
                     /translation="QRVVFPVAHGEGNYFADEETLDRLEGEGRVIFRYAPGENPNGSA
                     RDIAGILNEKRNVLGLMPHPERVCDPLLGGTDGRVLFESLIETLT"
     gene            269..2446
                     /gene="purL"
                     /locus_tag="VGC27_10900"
     CDS             269..2446
                     /gene="purL"
                     /locus_tag="VGC27_10900"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY0283118.1"
                     /translation="MTTITPQIVREHGLSEEEYARIKGLLGREPNYVELGVFSVMWSE
                     HCSYKSTRAHLKKLPTKGPRVIHGPGENAGVVDIGDGDVVVFKMESHNHPSFIEPYQG
                     AATGVGGIMRDVFTMGARPVASLNSLRFGEPSHSKTRHLVAGVVSGIGGYGNCMGVPT
                     VGGEVNFDRSYNGNILVNAMCVGLARADKIFLSAAKGAGNPVVYVGSKTGRDGIHGAT
                     MASAEFNEDSEEKRPTVQVGDPFTEKLVLEACLELMETDVIISIQDMGAAGLTSSSSE
                     MADKGGAGIVIDLDKVPQRETNMSAYDMMLSESQERMLAVLKPGREAEAERIFKKWEL
                     DFSVIGVTTDTHRLIVKHKGETVADMPVGSLSDAAPIYERPFVKRAPATGTPSFDASR
                     PVMESLLKLLGTPDMCSKRWVWEQYDTSVMADTVQQPGGDAAVVRVHGTDKGLAITTD
                     VTPRYCRADPYEGAKQAVAEAWRNITAVGALPLAVTDCLNYGNPEKPEIMGEIVAGID
                     GIGDACRALDFPIVSGNCSLYNETQGEGILPTPTIGGVGLLKDVHRMATIAFKREGDA
                     IVLIGETKGHLGQSVYLREVEGKEEGACPPVDLALEKKNGDFVRALIEAGRVDTVHDV
                     SDGGPLVAVAEMALAGNIGATVASGTIPFLFGEEQGRYVLAAPRAAAERIVSEAKEAG
                     IAASLLGTTGGDKVAVKEAGEMALARLRKAHEGWLPSYMAGDI"
     gene            2446..2679
                     /locus_tag="VGC27_10905"
     CDS             2446..2679
                     /locus_tag="VGC27_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEY0283119.1"
                     /translation="MAMTAGEIEKLIKQAFPDATVTVTPLADDNDHYAAMVTSRQFKG
                     KTRVQQHQMVYDALGGRMGGVLHALSLQTAAKD"
     gene            2748..3080
                     /gene="grxD"
                     /locus_tag="VGC27_10910"
     CDS             2748..3080
                     /gene="grxD"
                     /locus_tag="VGC27_10910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY0283120.1"
                     /translation="MASVHDRIAADVASSDVVLFMKGTPVFPQCGFSAAVVQILSELG
                     VKFKAVDVLKDADISEGIKLFSNWPTIPQLYIKGEFVGGCDILREMNETGELKALFAD
                     KGVVLANA"
     gene            complement(3226..4413)
                     /locus_tag="VGC27_10915"
     CDS             complement(3226..4413)
                     /locus_tag="VGC27_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283121.1"
                     /translation="MTTRRTAIAGGIGAALLAALGYRAWDRGAFSSGKGDAYAPWQEW
                     RGHAGEGVRRPLHAAILAANAHNSQPWLFAPSENAITVYADRRRNLGAFDPFRREMHL
                     SLGCAVENLVIAAGALGFSAQIVPEQGELTLSPSPDPVPAAHIVFSPGRYRFRALHDF
                     VPLRHTNRGPYDPQRPISAEALHRLIDFGTGAETRVVCFSDAKARAELAALIVEATQR
                     IIRDEDMSEDSARWFRTGHREILAHRDGVTTDTAGLSPAMAVAAKLLPDQDARSADKY
                     WLAATRDVQAPSAAAFGMVLVMNRLDMAGAIAAGRAWQRMHLVATANGYAAQPLNQPV
                     EMADHDRALGNPDSFGRALREISGVQDWEAAFVFRLGYPERPALPSPRRPLNEVIRQT
                     GLA"
     gene            complement(4443..>4853)
                     /locus_tag="VGC27_10920"
     CDS             complement(4443..>4853)
                     /locus_tag="VGC27_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283122.1"
                     /translation="IVAERHAPAGLLPPLGTPERAQALRWIAFMASEIYPIVEIFDYP
                     ERFVPKGAEAEALRLKARDRVRARMLLVEQAIAGPWLLASGFSLVDVYAAMFSRWSEC
                     RDWRDEHLPKIMAIAAALSRHPSIAPVWAKHFAP"
BASE COUNT          921 a         1551 c         1566 g          815 t
ORIGIN      
        1 gtcagcgcgt agtctttccg gtggcgcatg gcgagggcaa ttacttcgcc gacgaggaaa
       61 ccctcgaccg gctggaaggc gaaggccgcg tgatcttccg ctatgcgccg ggcgaaaatc
      121 ccaacggttc ggcgcgcgat atcgccggta tcctcaacga gaagcgcaac gtgctgggcc
      181 tgatgcccca ccccgagcgg gtctgcgatc cgctcttggg cggcaccgac ggccgcgtac
      241 tgttcgaaag cctgatcgaa acgctgacat gacaaccatc acgccccaaa ttgtccgcga
      301 gcacggcctc tcggaggaag agtatgcgcg catcaaaggc ctgctcggcc gcgagcccaa
      361 ttatgtcgag ctgggcgtct tctcggtgat gtggagcgag cactgctcct acaaatccac
      421 ccgcgcccat ctcaagaagc tgccgaccaa agggccgcgc gtcatccacg gccccggcga
      481 gaatgccggc gtggtcgata tcggcgacgg cgacgtggtg gtcttcaaga tggagagcca
      541 caaccatccc tcctttatcg agccgtacca aggcgcggcg accggcgtgg gcggcatcat
      601 gcgcgacgtc ttcaccatgg gcgcgcggcc ggtggcgtcg ctgaactcgc tgcgcttcgg
      661 cgagccctcc cactccaaaa cacgtcatct ggtggcgggc gtggtctccg gcatcggcgg
      721 ctacggcaat tgcatgggcg tgcccaccgt cggcggcgaa gtgaatttcg accgttccta
      781 taacggcaac atcctggtca acgccatgtg cgtgggcctg gcccgcgccg acaagatctt
      841 tctgtccgcc gccaagggcg cgggcaatcc ggtggtttat gtcggttcca agaccgggcg
      901 cgacggcatt cacggcgcca ccatggcgag cgcggagttc aacgaagatt ccgaggagaa
      961 acgcccgacg gtgcaagtcg gcgatccgtt caccgagaag ctggtgctcg aggcttgcct
     1021 cgaactgatg gaaaccgacg tcatcatctc cattcaggat atgggggccg cgggcctcac
     1081 ctcctcttct tcggaaatgg cggacaaggg cggcgcgggc atcgtcatcg acctcgacaa
     1141 ggtgcctcag cgcgaaacca atatgagcgc ctacgacatg atgctgtcgg agagccagga
     1201 gcgcatgctg gcggtgctga aacccggccg cgaagccgaa gccgagcgca tcttcaagaa
     1261 atgggaactc gatttctcgg tgatcggcgt caccaccgac acgcaccggc tgatcgtcaa
     1321 gcacaagggc gaaaccgtcg ccgatatgcc ggtgggttcg ctgtcggacg cggcgcccat
     1381 ctatgagcgg cccttcgtca agcgcgcgcc ggccacggga acgccttcgt tcgacgcctc
     1441 gcgcccggtg atggagtcac tgcttaagct tctcggcacg ccggacatgt gttccaagcg
     1501 ctgggtgtgg gagcagtacg acacctccgt catggccgac acggtgcagc agcccggcgg
     1561 cgacgcggcc gtggtgcgcg tgcacggcac cgacaagggc cttgcgatca ccacggatgt
     1621 gacgccgcgt tattgccgtg ccgatcccta tgagggcgcc aagcaggccg tggccgaggc
     1681 ttggcgaaac atcactgccg tcggcgcctt gccgctggcc gtcaccgatt gcctcaacta
     1741 cggcaatccc gagaagccag aaatcatggg cgagatcgtg gcgggcatag acggcatcgg
     1801 ggacgcctgc cgcgcgctcg attttcccat cgtctcgggc aattgctcgc tctacaacga
     1861 gacccagggc gaaggcattc tgccgacgcc caccatcggc ggggtcggcc tgctgaagga
     1921 tgtgcatcgc atggcaacca tcgctttcaa acgcgagggt gatgccatcg tcctgatcgg
     1981 cgagacgaaa ggccatctcg gccaatccgt gtatttgcgc gaagtggaag gcaaggaaga
     2041 aggtgcctgc ccgcccgtcg atctggcgct ggagaagaag aacggcgatt tcgtgcgcgc
     2101 gctgatcgag gccggccgtg tcgatacggt gcatgatgtt tccgacggcg ggccgcttgt
     2161 tgccgtcgcg gaaatggcgc tggccggaaa catcggcgcc accgttgctt ccggcacgat
     2221 cccattcctg ttcggcgaag agcagggccg ctatgtgctg gccgcgccac gcgccgccgc
     2281 agaacgcatt gtctccgaag cgaaagaggc gggcattgcg gcttctttgc ttggcacgac
     2341 gggcggggat aaagtcgccg tgaaagaggc gggcgagatg gcgctcgccc gcttgcgcaa
     2401 ggcgcatgaa ggctggctgc cgtcctacat ggctggagat atctgatggc gatgaccgcc
     2461 ggcgaaatcg agaagctgat caaacaggct tttcccgatg cgacggtgac ggtcacgccc
     2521 ctcgccgacg acaatgacca ttacgccgcc atggttactt cgcggcagtt taagggcaag
     2581 acgcgcgtgc agcagcatca gatggtttac gacgcgctgg gcgggcgcat gggcggcgtg
     2641 ctccatgcgc tgtcgctgca aaccgcggcg aaggactgac tgcgcttgac gcttcgcggc
     2701 gccgctccta tttcttgggc ccgaccctat agcgaaagcg cgcgaaaatg gcctctgtcc
     2761 acgaccggat tgccgcagac gtcgcctcca gcgatgtcgt gctgttcatg aaaggcacgc
     2821 cggtgtttcc gcaatgcggc ttctccgccg ccgtggtgca gattctttcc gaacttggcg
     2881 tcaagttcaa agccgtggac gtgctgaagg acgcggatat cagcgagggc atcaagctgt
     2941 tctcaaactg gccgacgatc ccgcagcttt acatcaaggg cgaattcgtc ggcggctgcg
     3001 acatcctccg cgagatgaac gagaccggcg agttgaaagc gctgttcgcc gacaagggcg
     3061 tcgtgctggc gaacgcctaa agcttcgtac gttcagacga gatcacgttg tcttcatgct
     3121 tcgacagtcg gtagcaacag cgtgccgacc agcatgaggg ggagaaaacc ctcaccctga
     3181 gcttatcgaa gggtgtgggg cgattccgtc tgaatgcatt ttgctctaag ccaatcctgt
     3241 ttgacggata acctcgttca aaggccgccg cggcgagggc aaggccggac gttcgggata
     3301 gccgagacga aagacaaacg cggcttccca atcctgcaca cccgaaattt cccgaagggc
     3361 gcggccgaaa ctgtccgggt ttcccagcgc ccggtcgtgg tcggccattt cgaccggctg
     3421 attgagcggc tgggcggcat aaccgttcgc cgtcgccacc agatgcatgc gctgccaggc
     3481 gcggcccgcc gcgatggcgc ccgccatgtc gaggcggttc atcaccagca ccatgccgaa
     3541 ggcggcggcg ctcggcgcct gcacatcgcg cgtcgccgcc agccaatatt tatcggccga
     3601 ccgcgcgtcc tggtcgggca atagcttggc cgccacggcc atcgcgggcg aaagccccgc
     3661 ggtatcggtg gtaacgccgt cgcggtgcgc caggatttcc cggtggccgg tgcgaaacca
     3721 gcgggcgctg tcttcggaca tgtcctcgtc gcgaatgatg cgttgcgttg cctcgacgat
     3781 gagcgcagcc aattccgcgc gggccttggc atcgctgaag cagaccacgc gcgtctcggc
     3841 gccggtgccg aaatcgatca ggcgatggag cgcctccgcc gatatcgggc gctgcggatc
     3901 gtaggggccg cgattggtat ggcgaagcgg gacaaaatcg tgcaacgccc gaaagcgata
     3961 gcgccccggc gaaaagacga tgtgcgcggc gggaaccgga tcgggcgacg gcgatagggt
     4021 cagctccccc tgttcgggaa caatctgcgc tgagaatccc aaagcccctg ccgcaatcac
     4081 cagattttcc accgcgcacc ccaggctcag atgcatctcg cggcggaacg gatcgaaagc
     4141 gccgaggttg cggcggcgat ccgcatagac ggtgatcgca ttctccgacg gcgcaaacag
     4201 ccagggttgc gaattatgcg cgttggcggc gaggatcgcg gcgtggagcg ggcggcgcac
     4261 gccctcgccc gcatgtcccc gccattcctg ccatggcgcg taggcatcgc ctttcccgct
     4321 cgaaaacgcg ccacggtccc aggcgcggta accaagcgcg gccaacaagg cagcgccgat
     4381 tccgccggca atcgctgtcc tacgcgtcgt catgcgcgcc tccgcaaacc ccggcgagcg
     4441 tatcacggtg cgaaatgctt cgcccagacc ggcgcgatgg acgggtgccg cgacagcgcc
     4501 gccgcaatcg ccatgatctt cggcaggtgt tcgtcgcgcc aatcccggca ttccgaccag
     4561 cggctgaaca tcgccgcata aacatcgaca agggaaaagc cgctcgccag cagccacggc
     4621 ccggcaatcg cttgctccac cagcaacatg cgcgcccgca cgcgatcacg cgccttcagc
     4681 cgcagcgctt ccgcctcggc gcctttgggc acgaagcgct cgggataatc gaagatctcg
     4741 acgatcggat agatttcgga cgccatgaac gcaatccaac gaagggcctg ggcgcgctcc
     4801 ggcgtgccaa gaggagggag aagccccgcc ggcgcatggc gttcggcgac gat
//
LOCUS       DASWPU010000514         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_53230,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000514 DASWPU010000000
VERSION     DASWPU010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53230"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1612
                     /locus_tag="VGC27_10925"
     CDS             <1..>1612
                     /locus_tag="VGC27_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEY0283123.1"
                     /translation="LWGAIVIAFCTPGLAQTSAEPGHVDERFRQPPPPPTLGAPVEIP
                     IAPQSVAPSDSQKVPFTLSGVSFEGNNVLPTSQLQALAASYVGRSITLADVYELADRI
                     TAAYRAAGYILVRAIVPAQTVSSGHLTLRIVEGYIDQVKVQGDAGGAKRYLEAYGRRI
                     AAAKPLTAKVLERELLLASDISGFNVRSVLTPSATVQGAADLTLVVERKPVDAFLSVD
                     NRGSKFLGPYQVMGGVFFNDVFGTAGRFGINAVVTPDQGPDLAYGAVSYNQPIGINGL
                     RLFASASYVQTRPGSVLRALDTKGRATNADLALSFPFVRSRDFNLVGSLGAAYHDVQS
                     SNFSASPLFSDHVRSLNANVFLNALDDWGGYSTASASVTQGLEILGATRSGSTVKSRV
                     GASGDFTRANFEVTHEQPLISRFSAMVGLGAQTSFGGSLLSSEQFSLGGSNYNRAFDP
                     SEVTGDSALAGKAELRWDILDRASIVSGVQLYGFYETGEVWQAKALPGTPKSQTLSST
                     GAGIRFAVADHFSTDLEWAKPLNTDVLGA"
BASE COUNT          270 a          494 c          515 g          333 t
ORIGIN      
        1 ctttgtgggg cgcgatcgtg attgcgttct gcacgccggg gctggcgcaa acatctgcag
       61 agcccggcca cgttgacgag cgtttccgac agccgccgcc accgcctact ctcggcgcgc
      121 cggtggagat tcccatcgcc ccgcagagtg tggccccgtc ggacagccaa aaagtgccgt
      181 tcacgctttc gggtgtgagc ttcgaaggca acaatgtttt gcccaccagc cagcttcagg
      241 cgctggccgc aagctatgtc gggcgaagca tcacgctggc tgacgtctac gagcttgccg
      301 atcgcataac ggctgcctat cgcgccgccg gttacatctt ggtgcgcgcc atcgtacccg
      361 cgcagacagt cagcagcggg cacctgacgc tgcggatcgt cgaaggttat atcgaccagg
      421 ttaaggtcca gggcgatgcc ggcggggcga agcgctacct ggaagcctat ggacggcgca
      481 tcgcggcggc gaaaccgctt acggcgaaag tgctcgaacg cgaattgctg ctggcgagcg
      541 atatcagcgg attcaatgtc cgcagcgttc tgacgccttc ggctacggtg cagggcgcgg
      601 ccgatcttac cctcgtcgtc gaacgcaagc cggtggacgc tttcctgtcg gtggacaatc
      661 gcggctccaa attcctcgga ccttatcagg tgatgggggg cgtatttttc aacgacgttt
      721 tcggcactgc cggccgcttt ggcattaatg ccgttgtgac gccggaccag ggcccggatt
      781 tggcttacgg cgcggtcagc tacaaccagc ccatcgggat aaatgggctg cggctttttg
      841 catccgccag ctatgtgcag acgcggcccg gctcggttct gagggcgctg gacaccaagg
      901 gccgcgccac gaacgccgat ctggcgttgt cctttccgtt cgtgcgctcg cgcgatttca
      961 atctggtggg gtcgctgggg gctgcctatc acgacgtgca gtcgagcaat ttcagtgctt
     1021 cgccgctgtt cagcgatcac gtccgttccc tgaacgccaa cgtgttcctt aatgctctgg
     1081 acgattgggg cggttattcc acggcgtcgg ccagcgtcac tcaaggcctc gaaattcttg
     1141 gcgcaacgcg tagcggctcg acggtcaaat cgcgcgtcgg cgccagcggc gatttcacgc
     1201 gcgcgaactt cgaggtcacc cacgagcagc cgttgatcag ccgtttttcg gcgatggtgg
     1261 gtctcggcgc gcagacttcg ttcggcggtt cgttgttgtc ctccgagcag ttctcgctgg
     1321 gcggtagcaa ctacaaccgt gccttcgatc cgtccgaggt gacgggggat tccgcgctgg
     1381 ccggcaaagc ggagctgcgc tgggatattc tcgaccgcgc ctcgatcgtc tccggcgtcc
     1441 agctctatgg cttctatgaa accggcgagg tctggcaggc caaggcgctg ccggggacac
     1501 cgaaaagcca gacattgtct tcgacgggag ccggtattcg ctttgccgtc gccgaccatt
     1561 tcagtacgga cctggaatgg gccaaaccgc tgaataccga tgtgttgggg gc
//
LOCUS       DASWPU010000515         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_84323,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000515 DASWPU010000000
VERSION     DASWPU010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84323"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..992)
                     /gene="rarD"
                     /locus_tag="VGC27_10930"
     CDS             complement(78..992)
                     /gene="rarD"
                     /locus_tag="VGC27_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEY0283124.1"
                     /translation="MSKVADDTPPNEAAGILYAGAAYSLWGLFPLYWALLSVVPPIEL
                     SIHRMLWCALFAAGVTAARGRLKHLLHILTAKKLIWPLALSSVLIAANWTIYIWSIAA
                     HHVVEAALGYYINPLLSIVLGVALLGERLSRLKLAAVVLAAVAVGVQAIDVGHVPWIA
                     LGLALTFGFYGYMRKLTPVDPLDGLTVETCLLFPLTLGTVLFLAWNGTGAFPAAHFST
                     DALLVLAGPVTGVPLVLFAAGARRLRMTTLGFLQYLSPTITLAVATLVFGEPFSATDA
                     ATFGCVWAALILVGLEGRFARPFARLAP"
     gene            complement(1057..>1763)
                     /locus_tag="VGC27_10935"
     CDS             complement(1057..>1763)
                     /locus_tag="VGC27_10935"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEY0283125.1"
                     /translation="GGLHSSAMLKDPRTYEHIPPETVGNARKILVSDQAGRSNLLVRL
                     ADAGIDVAAKDPRLSRLLEDIKQREFLGYAYDGAEASFELLARRALGKVPEYFDVESF
                     RVIVEKRHNALGKQITVSEATVRVEVDGEMLHNVGTGNGPVDALDDALRKDLGRYSTY
                     LTDLELVDYKVRILNGGTDAITRVMVESKDGKGERWSTVGVSENIVDASFEALVDSIY
                     YKLLRDGVRASVARDT"
BASE COUNT          348 a          599 c          547 g          269 t
ORIGIN      
        1 ttgcccggcc ccggctgctc gacctttaca ggcttgtcga tacgcccgaa gaagccatcg
       61 acgcgattgc ggccgcgtta gggtgctagc cgcgcgaacg gacgggcgaa ccgtccttcc
      121 aggcccacca ggatcagcgc cgcccaaacg cagccgaagg tcgccgcatc ggtggccgag
      181 aacggttcgc cgaagaccag cgtggcgacc gccagggtga tcgtgggcga aagatattgc
      241 aggaatccca aggtcgtcat gcgcaagcgc cgtgcgcccg cggcgaacag gacgagcggc
      301 acgcccgtca ccggtcccgc cagcaccagc agcgcatcgg tcgaaaaatg cgccgccgga
      361 aacgcgcccg tgccgttcca cgccaggaac agaaccgtgc ccagcgtgag cgggaacaat
      421 aggcaggttt ccaccgtcag cccgtccaac gggtccaccg gcgtcagttt gcgcatatag
      481 ccgtagaagc cgaaggtcag cgccaagccg agcgcgatcc acgggacgtg gccgacgtcg
      541 atggcctgaa cgccgacggc aacggccgcc agcacgaccg ccgcgagctt gagccgcgac
      601 agccgctcgc ccagcagcgc caccccgagc acgatcgaaa gcaaggggtt gatgtaatag
      661 cccagcgcgg cctcgacgac atgatgggcg gcgatgctcc agatatagat cgtccaattg
      721 gccgcgatca gcacgctcga cagcgccagc ggccagatca gctttttggc cgtcagaatg
      781 tgcaggaggt gcttgagccg cccacgcgcc gccgtcacgc cggcggcgaa cagcgcgcac
      841 cacagcatgc ggtggatcga caattcgatg ggcggcacga cggaaagcag cgcccaataa
      901 agcgggaaca ggccccacag cgaataggcc gcgccggcat aaagaatccc cgccgcctcg
      961 ttcggcggtg tgtcgtcagc aactttggac atggcggcaa tgtagacggt ctttggccgc
     1021 cgcgctcgtc gccgccatca aaccagccat gcgtttctac gtatcgcgcg cgaccgatgc
     1081 ccgcaccccg tcccgcagca atttgtaata gatcgaatcc accagcgcct cgaacgaggc
     1141 atcgacgatg ttctcggaga cgccgaccgt ggaccagcgt tcacccttgc cgtccttgct
     1201 ctcgaccatg acgcgggtga tcgcgtcggt gccgccattg aggattctga ccttgtaatc
     1261 gacgagttcc aaatcggtca gataagtgga atagcgcccc aaatccttgc gcaaggcgtc
     1321 gtccagcgcg tccaccggac cgttgccggt gccgacatta tgcagcatct cgccgtccac
     1381 ttcgacgcgc accgtcgctt ccgagaccgt gatctgcttg cccaaggcgt tgtggcgctt
     1441 ctcgacgatg acgcggaagc tttcgacgtc gaaatactcc ggcaccttgc ccaaggcgcg
     1501 ccgcgccaga agctcgaacg aagcctccgc gccgtcatag gcgtagccca ggaactcgcg
     1561 ctgcttgatg tcctccaaca ggcgcgacag gcgcgggtcc ttggcggcca cgtcgatgcc
     1621 ggcgtcggcc agccgcacca gcagattgga gcgcccggcc tgatcggaga ccagaatctt
     1681 ccgcgcgttg ccgaccgttt ccggcgggat atgttcgtag gtgcgcgggt ccttcagcat
     1741 ggcggaagag tgcagcccgc cct
//
LOCUS       DASWPU010000516         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_41835,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000516 DASWPU010000000
VERSION     DASWPU010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41835"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VGC27_10940"
     CDS             complement(<1..1172)
                     /locus_tag="VGC27_10940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEY0283126.1"
                     /translation="MKIINALSQLVREYMTLAKAGVLALWTRFAAWAVKLLTPLLDVP
                     VVAGIAHHVEVAKESFAIERERSLTAKKMAETAFLPAALEVMETPPNPVGRAILWIMI
                     GFLVIAGLWAGFGKVDEVAIAPGKLIPRGEVKLIQAPDYGIVRAIHIVDGQLVKKGEP
                     LIELDPTVSGAEAEQARKSLLVARTDQVRARALAEHGGPDEAGTFAPPEGVDPESAQT
                     QKALVEEKIREHDAAHAALMQEIAQHKSELAMVEAEVEKLKAQLPLAEYQYEAIRGLA
                     DEGYAPKLRVMELQERVIGLRQDLAIRRAEATKVRAVIRGGEQQLAKLDSEFKRQALD
                     ALNEAEAAVSLRGEELKKAEEKSKLTVLKAPEDGIVQQLAVHTLGAVVKPADALLVV"
     gene            complement(1169..>1735)
                     /locus_tag="VGC27_10945"
     CDS             complement(1169..>1735)
                     /locus_tag="VGC27_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0283127.1"
                     /translation="PERGRVLVDGVDLALVDPSWLRRQVGIVLQENILFNRTVRDNIA
                     LADPAMPMENVTMAAELAGAHEFILEFPNAYDTRIDERGSNLSGGQRQRIAIARALAT
                     HPKILIFDEATSALDAESEDVIQRNLKAMTAGRTVIIIAHRLSAVRQADRIVTLEKGE
                     ITEAGTHQELLQRNGRYASLYRKQMGIA"
BASE COUNT          279 a          564 c          543 g          349 t
ORIGIN      
        1 acgacgagca gcgcatcggc gggcttgacc accgcgccca aggtgtggac ggcaagctgc
       61 tgcacgatgc cgtcttccgg cgccttcaga acggtgagct tgcttttctc ctcggccttc
      121 ttgagttctt caccgcgcag cgagacggcg gcctcggctt cgttcagcgc gtccagggcc
      181 tggcgcttga attcgctgtc gagcttggcg agctgctgtt cgccgccgcg gatcacggcg
      241 cgcaccttcg tggcttccgc ccgacggatc gcgaggtcct gccgcaagcc gatcacgcgc
      301 tcctgcaatt ccatgacgcg cagcttcggc gcgtagccct cgtcggcgag gccgcggatc
      361 gcctcatact gatattcggc cagcggcagt tgcgccttga gtttctccac ttcagcctcg
      421 accatcgcca gctcgctttt gtgctgggcg atctcctgca tgagggcggc gtgggccgcg
      481 tcatgttcgc gaattttctc ttcaaccagc gctttttgcg tttgcgccga ttcaggatcg
      541 acgccttccg gcggggcgaa cgtaccggcc tcgtcgggtc cgccgtgttc cgcaagcgca
      601 cgggcgcgca cctgatccgt tcgcgcgacg agcagcgatt tgcgcgcctg ttcggcctcg
      661 gctcccgaga ccgtagggtc cagctcgatc agaggctcgc cctttttgac caactggccg
      721 tcgacaatat ggatcgcccg cacgatgcca taatccggcg cctggatcag ctttacttcg
      781 ccgcgcggaa tgagcttgcc cggcgcgatg gccacctcat cgaccttgcc gaaacccgcc
      841 cacagccccg cgatgacaag aaagccgatc atgatccaca gaatcgcgcg gccgacggga
      901 ttgggcggcg tctccataac ctccagcgca gcgggcagga aagcggtttc ggccattttc
      961 ttggccgtca ggctgcgttc gcgttcgatt gcgaagctct ctttggccac ctcgacatgg
     1021 tgagcaatgc cggcgacgac gggcacatca agcagcggcg tgagaagttt gaccgcccac
     1081 gccgcgaacc gcgtccacag cgccagaaca cctgctttgg cgagcgtcat gtactctcga
     1141 acgagctgcg acagggcgtt gatgatcttc atgcaatccc catctgcttt ctgtagagag
     1201 aggcataccg tccgttccgc tgcagcagct cctggtgggt tccggcttcg gtgatctcgc
     1261 ctttttcaag agtgacgatg cggtcggcct ggcgtacggc cgaaagcctg tgcgcgatga
     1321 tgatgacggt gcggccggcg gtcatggctt tgagattgcg ctgaatgacg tcttcgctct
     1381 cggcgtcgag cgccgaggtc gcctcgtcga agatcaggat cttcgggtgg gtcgccaggg
     1441 cgcgtgcaat cgcgatacgc tgccgctgtc cgccggacag gttggagccg cgctcgtcga
     1501 tgcgggtgtc gtaggcgttg ggaaactcaa gaatgaactc gtgcgcgccc gcaagttccg
     1561 ccgccatcgt cacgttctcc atcggcatgg cgggatcggc gagtgcaatg ttgtcccgaa
     1621 ccgtgcgatt gaaaaggatg ttctcctgca acacgattcc gacctgccgg cgcaaccagg
     1681 acggatcgac gagcgccaga tcgacgccgt cgaccaggac gcgcccgcgc tcggg
//
LOCUS       DASWPU010000517         1836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_16953,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000517 DASWPU010000000
VERSION     DASWPU010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16953"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VGC27_10950"
     CDS             complement(<1..669)
                     /locus_tag="VGC27_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0283128.1"
                     /translation="MPDGTILIADDDSAIRTVLNQALGRAGYDVRTTGTAAGLWRWVA
                     AGDGNLVITDVILPDENGFDLIPRIKRLRPDLPIVVMSAQNTILTAITAAERGAFDYL
                     PKPFDLKDLTAVVQRALASPQVRREPAPDEEGTEGLPLIGRSAAMQEIYRVIARLTQT
                     DLTVMIMGESGTGKELVARALHDYGKRRHGTFVAVNMAAIPKELVESELFGHERGAFT
                     GATNR"
     gene            complement(662..1777)
                     /locus_tag="VGC27_10955"
     CDS             complement(662..1777)
                     /locus_tag="VGC27_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0283129.1"
                     /translation="MSATAKLPPEIPASSQIAAALPMPVLVVGPQQQIVFVNPAAEQF
                     FDMGSGHLLKQKLTDLIPFGSPLIQLIGQAIERRASVGERVVDLSTPRHGERIADVMV
                     TPLIDPEGTVVITLQERSLAQRIDRQLLHRGAVRSMHGMAAVLAHEIKNPLAGIRGAA
                     QLIEEHLPAHERDLTQLICAEADRIRALIDRMESFGDTRSFTRVPVNIHEVLDRVRRV
                     AETSFAKNVSFIEDFDPSLPRVLGDRDQLIQVFLNLVRNAADALPESSGEITLTTGYR
                     PGVRFGAAVAGERLSLPLQVTVKDNGAGVPADLMPYIFDPFVTTKRGGSGLGLALVAK
                     IIGDHGGVIECDSVPRRTIFRILLPKAANDSERSPNA"
BASE COUNT          294 a          568 c          630 g          344 t
ORIGIN      
        1 gcgattggtg gcgccggtaa aggcgccccg ttcatgcccg aacaattcgc tttcgaccag
       61 ttccttggga atcgccgcca tgttcaccgc gacgaaggtg ccgtgccggc gtttgccgta
      121 atcgtgcagc gcgcgcgcga caagctcctt gccggtgccg ctttcgccca tgatcatcac
      181 ggtgaggtcg gtttgggtga ggcgcgcgat aacgcgataa atctcctgca tcgccgccga
      241 gcggccgatc agcggcagtc cttcggtgcc ctcttcgtcg ggggcgggct cgcgtctgac
      301 ctgcgggctg gccaatgcgc gctggacgac ggccgtcagg tccttcaggt cgaagggctt
      361 gggcagatag tcgaaggcac cgcgctccgc cgcggtgatc gcggtcagga tcgtgttctg
      421 cgcgctcatc accacgatgg gcagatctgg ccgcaggcgc ttgatgcgcg ggatgaggtc
      481 gaagccgttt tcatcgggca ggatgacatc ggtgatgacg agattgccgt cgcccgccgc
      541 cacccagcgc cacaggccgg cagcggtgcc ggtggtgcgc acgtcgtagc cggcgcggcc
      601 gagcgcctgg ttcaacaccg tgcggattgc cgaatcgtcg tcggcgataa ggatcgtgcc
      661 gtcaggcatt ggggcttctt tcgctgtcgt tggccgcttt cggcaacaag atgcggaata
      721 tggtccggcg cggcacggaa tcgcactcga tgacgccgcc atggtcgccg atgatcttgg
      781 cgaccagcgc cagcccgagg cccgagccgc cgcgcttggt cgtcacgaac ggatcgaaga
      841 tgtagggcat caggtcggcg ggcacgccgg cgccgttgtc tttcacggtc acttgcagcg
      901 gcaggctcag ccgctctccc gccaccgccg cgccgaagcg cacgccggga cgatagccgg
      961 tcgtcagcgt gatctcgccg ctgctttccg gcagtgcgtc ggcggcgttg cgcaccagat
     1021 tcaggaacac ttggatgagc tggtcgcggt ctcccagcac gcgcggcaaa gacggatcga
     1081 agtcctcgat gaaactgacg ttcttggcga agctggtttc ggcgacgcgc cgcacgcggt
     1141 cgagcacttc gtggatgttc accggcacgc gcgtgaaaga acgcgtatcg ccgaaggact
     1201 ccatccggtc gatcagcgcg cggatgcggt cggcttccgc acagatgagc tgggtgaggt
     1261 cgcgttcatg cgccggcagg tgctcttcga tcaactgcgc cgcgccgcga atgcccgcca
     1321 gcggattctt gatctcgtgc gccagcacgg ccgccatgcc gtgcatcgag cgcaccgcgc
     1381 cgcggtggag cagttgccgg tcgatgcgtt gcgccaggct gcgttcctgc agcgtgatga
     1441 cgactgtgcc ttccgggtcg atcagcggcg tgaccatgac gtcggcaatg cgctcgccgt
     1501 gccggggcgt ggagagatcg acgacgcgct cgccgacgga ggcgcggcgc tcgatcgcct
     1561 gtccgattaa ttggatcagc ggacttccga acggtatcaa gtcggtgagc ttctgcttca
     1621 gcaggtggcc ggagcccatg tcgaagaact gctcggcggc ggggttcaca aaaacgattt
     1681 gttgttgcgg accgacaacg aggacaggca tcggcagcgc ggcggcgatc tggctcgacg
     1741 cggggatttc tggcggcagc tttgcggtgg cgctcacttg gataccgtgc ctaatactta
     1801 ggcatcatag tacgttgccg attatgtgtg caacta
//
LOCUS       DASWPU010000518         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_108899,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000518 DASWPU010000000
VERSION     DASWPU010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108899"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VGC27_10960"
     CDS             <1..706
                     /locus_tag="VGC27_10960"
                     /inference="COORDINATES: protein motif:HMM:NF023203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283130.1"
                     /translation="EFRDTVVTLLLDNSGSMRGRPIMVAAMCADILAHTLERCAVKVE
                     ILGFTTRAWKGGQSREKWIAEGKPPHPGRLNDLRHVVYKKADEPWRRARRNLGLMMRE
                     GLLKENIDGEALMWAYGRLIARSEQRKIMMVISDGAPVDDSTLSVNAGNYLERHFRKV
                     IDDIENKSSIELVAIGIGHDVTRHYRRAVTIVDAEQLGGAMTDQLIGLFAEKQGEPSR
                     RTLRANGRRQPVARRT"
     gene            797..>1622
                     /locus_tag="VGC27_10965"
     CDS             797..>1622
                     /locus_tag="VGC27_10965"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEY0283131.1"
                     /translation="MTLLFSAAAFAQAQQLTFTPFHSNGIYALGEKVGWTVGLPDGAA
                     PSPARYTYTIKTNAMDVAGTGSFDLSSGSATIETSADAPAMLHVTVDRAAASPLSAAE
                     IDKINNSLKALLAKNDSAIKSIFEKHPDLGIVTAPSAEGAPARDHVATLGAAVAPTEL
                     QPSVPRPDDFDAFWAEKLAALGKIPVNPVLVPTPTTQAGVELYTVTLDSVGSHVRGYL
                     AKPNKTGKFPALIIYQWAGVYALQPDWATNRAAEGWLVFNVNSHDLPPDQATGVPND"
BASE COUNT          330 a          526 c          492 g          274 t
ORIGIN      
        1 ggaattccgc gacaccgtcg ttaccctgct gctcgacaat tccgggtcga tgcgcggccg
       61 gccgatcatg gtggcggcga tgtgcgcgga catcctggcg cacacgctgg agcgctgcgc
      121 ggtgaaagtg gaaatcctcg gtttcaccac ccgcgcctgg aaaggcggac agagccgcga
      181 gaaatggatc gccgaaggca agccgccgca tcccggccgg ctgaacgatc tgcgccatgt
      241 cgtctacaag aaggccgacg agccgtggcg gcgggcgcgg cgcaacctcg gcctgatgat
      301 gcgcgagggc ctgctcaagg agaacatcga cggcgaagcc ctgatgtggg cctacggccg
      361 cttgattgcg cgctccgaac aacgcaagat catgatggtg atttccgacg gcgcgccggt
      421 ggacgattcg accttgtcgg tgaacgccgg caactacctg gagaggcatt tccgcaaggt
      481 gatcgatgac atcgaaaaca aatcgagcat cgagctggtg gcgatcggca tcggccacga
      541 cgtcacccgc cattaccgcc gcgcggtcac catcgtcgat gccgaacagc ttggcggcgc
      601 gatgaccgat caactgatag gattgtttgc ggaaaaacag ggggagccga gccgccggac
      661 gcttcgtgcc aacggccggc gccagccggt agcccgaagg acatagtttc aagcctcgta
      721 ggcgccaggt aaatctcaag gtgaaaccat gaaacatacc actctacgca ataagctccg
      781 tctgttggcg gcaagcttga cgttgctttt cagcgcggcg gcattcgccc aagcccagca
      841 acttaccttc acgccgtttc attcgaatgg gatttacgcg cttggcgaaa aagtgggctg
      901 gacggtcggc ctgcccgacg gcgccgcgcc gtcaccggcc cgctatacct acacaatcaa
      961 gacgaatgcc atggacgtgg ccgggaccgg cagtttcgat ctgtcgtccg gcagcgcaac
     1021 gattgaaacc agcgccgatg cgcccgccat gctgcatgtt acggtggaca gggcggcggc
     1081 gtccccgctt tccgccgccg aaatcgacaa gataaataac agcctcaagg cgttgcttgc
     1141 aaagaacgat tccgcaatca agtcgatctt cgaaaagcac ccggatcttg ggattgtaac
     1201 cgctccgtcc gccgagggcg cgcccgcccg ggaccatgtc gccacgcttg gcgcggccgt
     1261 cgctcccacc gaacttcagc cgtccgttcc tcgccccgac gacttcgacg ccttctgggc
     1321 ggagaaactc gcggcactcg ggaaaatccc cgtcaacccg gttctcgtgc ccacgccgac
     1381 cacgcaggcc ggcgtcgaac tctacacggt gacgttggac agtgtcggct cgcacgtccg
     1441 cggctacctc gccaagccga acaagacggg caagttcccg gcactcatca tctatcaatg
     1501 ggcgggcgtt tatgcgttgc aaccggactg ggccaccaac cgcgcggccg agggctggct
     1561 ggtgttcaac gtcaattcgc acgaccttcc gcccgatcag gccaccggcg ttccgaatga
     1621 ct
//
LOCUS       DASWPU010000519         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_115099,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000519 DASWPU010000000
VERSION     DASWPU010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115099"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..285
                     /locus_tag="VGC27_10970"
                     /pseudo
     CDS             136..285
                     /locus_tag="VGC27_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            307..477
                     /locus_tag="VGC27_10975"
     CDS             307..477
                     /locus_tag="VGC27_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283132.1"
                     /translation="MPLALPAPSLADPATSCSCGPGDKIEIVLEGGRRIVVGKDVDAT
                     ALRRIIEVLERK"
     gene            474..821
                     /gene="tnpB"
                     /locus_tag="VGC27_10980"
     CDS             474..821
                     /gene="tnpB"
                     /locus_tag="VGC27_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY0283133.1"
                     /translation="MIPVPSGVHVWIATGHTDMRKGFNGLALSVQEVLKRDPHSGNLF
                     VFRGKRGDLVKLLWYDGQGMCLFAKRLERGRFIWPSMAGEAVTISAAQLGYLLEGIDW
                     RAPQRTYRPELAG"
     gene            887..>1513
                     /locus_tag="VGC27_10985"
     CDS             887..>1513
                     /locus_tag="VGC27_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEY0283134.1"
                     /translation="MNLAALPTDVEALQNLVREQAALLQSKDLEIERLTARIVKLRRL
                     QFGRSSEKIDREIEQLELLLDDLHENEGARAERPVVTSEAAHKPARRPLPDHLPREEV
                     VHVDACVCPKCGGELRQLGEDVSEVLEYVPASFKVIRHVRPKFSCRRCEAITQAPMPS
                     LPIERGRPGPGLLAHVLVSKYADHLPLYRQSEIYAREGLDLERSTLADW"
BASE COUNT          281 a          431 c          503 g          298 t
ORIGIN      
        1 gtaagcgtcc ggtcttggcc gccttgtgtt gtggttaggc gagtagacga taggtcttgt
       61 tgaatcgata ggacctatat cgtgcctatg tctggacata gttctgtggc ggaggttgag
      121 gttattgcct cgacgcggcg aaggtggtcg gaagctgaga ggcgaacgat cctcgcggag
      181 gctggccagg acgggataac agtctctgcg gttgcgcgcc ggcatgggct atcgccgagt
      241 cttctgttcc ggtggcggcg tgagttcaag caatcgaaga agagggaggc gcccgagccg
      301 gcttttgtgc cccttgcgct gcccgcgcct tcattggccg acccggcaac gtcctgctca
      361 tgcggtcccg gagacaagat cgagattgtt ctcgaaggcg gacggcggat cgtcgtgggg
      421 aaggatgtcg atgcgacggc gcttcggcgc atcatcgaag tgcttgagcg caaatgatac
      481 cggttccgtc tggcgtgcat gtctggatcg cgaccgggca cacggacatg cgcaaagggt
      541 tcaacggtct ggcattgagc gtccaggaag tgttgaagcg cgatcctcac tccggcaatc
      601 tcttcgtgtt ccgcggcaag cgcggtgatc tggtgaagct gctgtggtat gacggtcagg
      661 gcatgtgcct gttcgccaaa cgacttgagc gcggacgctt catctggccg agcatggctg
      721 gtgaagcggt aacgatctcg gcagcccagc ttggatattt gctggaaggg atcgactggc
      781 gcgcgccgca gagaacatat cgacctgagc ttgcaggata gcggtggatt gactcgcgac
      841 gatccatcct ctggaagccg atgaaccctg cattacgctt cgacacatga atctcgccgc
      901 cttacctacg gatgtcgaag cgctacagaa cctggtgcgc gagcaggcgg cgctgcttca
      961 atcgaaagat ctggagatcg aacgcctgac ggcgcggatc gtcaagctga ggcggctgca
     1021 gtttggccgc tcgtcggaga agattgatcg cgagatcgag cagttggaac ttcttctgga
     1081 tgatctgcac gagaacgaag gcgcccgagc tgaacgccca gtcgtcacgt ccgaagcggc
     1141 acacaagccc gcccgccggc cactgcccga tcatctgccg cgcgaggagg tcgtccatgt
     1201 cgacgcctgc gtctgcccga agtgcggcgg cgaactgcgc caactgggcg aggacgtgag
     1261 cgaggtgctg gagtacgtgc cggcgtcctt caaggtgatc cggcatgtgc ggcccaagtt
     1321 ctcctgccgg agatgcgaag cgatcaccca ggcgccgatg ccctcgctgc ccatcgaacg
     1381 cggccggccg ggaccggggc ttctggccca tgttctggtc tccaaatacg ccgatcacct
     1441 gccgctctac cgccagagcg agatttacgc ccgcgaaggc ctcgatctgg aacgctccac
     1501 gctggccgat tgg
//
LOCUS       DASWPU010000520         8626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_16958,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000520 DASWPU010000000
VERSION     DASWPU010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16958"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VGC27_10990"
     CDS             complement(<1..804)
                     /locus_tag="VGC27_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEY0283135.1"
                     /translation="MNNLRNLALWIAVALLLVLLFNLFQGSAQHTNTSVISYTQFNQE
                     VERGGVKKVTVKGDQVIGTMTNGQAFSTTVPTSDQSLWTTLRSHKVDISVAQADDGAF
                     SIVNILINWFPMLLIIAVWIFFIRQMQSGGGKAMGFGKSRAKLLTERTGRITFEDVAG
                     VDEAKDDLKEIVDFLKDPQKFQRLGGRIPKGVLLVGPPGTGKTLLARAIAGEANVPFF
                     TISGSDFVEMFVGVGASRVRDMFEQGRKSAPCLIFIDELDAVGRHRGHGG"
     gene            complement(969..1973)
                     /gene="tilS"
                     /locus_tag="VGC27_10995"
     CDS             complement(969..1973)
                     /gene="tilS"
                     /locus_tag="VGC27_10995"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY0283136.1"
                     /translation="MTVAGAPWPCAVAVSGGSDSLALMFLLRDWAKRAGLPPPVMLCV
                     DHSLRPESAGEARKVLRQAKAAGLPARILVRKGEAPRSDIEAAAREARYRLMGEWAKK
                     NSLKAIYVGHTRDDQAETFLLRLARGSGVDGLAAMRMVAPYPVKGFSGLMLVRPLLAF
                     GREELRDELRARKQVWLDDPMNADPHFARVRIRNAWPALEAMGLSKERVADAAAHLAR
                     ARAALDKVSQAVLARACRFDRTEALLDPAALTGAPHELGLRALAAVLMEVSENPYRPR
                     FERLERLFSRIADGSIGAGRTLHGCRIGPAPSARACFGRGTLLVRREGNRRKTMKNNG
                     "
     gene            complement(2005..2886)
                     /gene="ybgF"
                     /locus_tag="VGC27_11000"
     CDS             complement(2005..2886)
                     /gene="ybgF"
                     /locus_tag="VGC27_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY0283137.1"
                     /translation="MTGRLTGAAAALVLVSAFAGTAGATQTVSDAPARAQMRGVQLAG
                     LFGESDEEKAARQQHEDNQDAQIADLKQRVQDLEQSLQRLTGQNEQVSYSMQELRAKL
                     DSQQKDYDYKLCTLSAQLLGAGNKGGQFGESLLCNLGADAGAMPVGQPMLGSGGTLGT
                     LSQNAVPDARPQFEAAMNLLARAQYDEARAAFRTFADDNPKDALAPQAVYWVGDVAYV
                     QKDYGAAAQAFAEVIKKYPTSSRGPDSMLKLGQSLLALGQTKEGCLTLGSIKGKYRNA
                     SASVLSQAASARAISCK"
     gene            complement(3016..3522)
                     /gene="pal"
                     /locus_tag="VGC27_11005"
     CDS             complement(3016..3522)
                     /gene="pal"
                     /locus_tag="VGC27_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY0283138.1"
                     /translation="MKNISTGLKLAAIAFAFVLAGCETKPPPAPAPAPAPVVTPAPSV
                     IPGSAEDFRVNVGDTVHFDYDKYAILEVDKGTLQKQATWLAQYPSVRVTIEGNADERG
                     TREYNLALGARRANAVKEYLISLGVAAARIETISYGKERPICSESTESCWAQNRRGVT
                     AIAAGANS"
     gene            complement(3663..4964)
                     /gene="tolB"
                     /locus_tag="VGC27_11010"
     CDS             complement(3663..4964)
                     /gene="tolB"
                     /locus_tag="VGC27_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEY0283139.1"
                     /translation="MRKIGLAMAVALSLAASSPALAALKVDVNQGNIQPLPIAIPDFV
                     AGSSGDVAVSQDIAKVVRADLERSGLFKPLDPKSYIDKVTNINVVPNFANWRIITAQG
                     LVTGSAALQPDGRLRVDFRLWDVYGGSQMLGLQYFTQPANWRRISHMISDAIYQRITG
                     EKGYFDTRIVFISESGPALNRKKRLAVIDEDGANPSFLTNGYLVLTPRFNPTAQMIAY
                     MSYVVGKPRVYLFDMESGRQEKLGDFPNMTFSPRFSPDGNKVVMSLEQGGNSDIYVMD
                     LRTRAVARLTHDPGIDTSPSYSPDGKQIAFESDRGGSQQIYIMNADGSNQHRISFGQG
                     RNGTPVWSPRGDLIAFTKQAGSFRIWVMRPDGTGERMLSNGWQDEGPTWAPNGRVLMF
                     TRTIQGGRGSQIWSVDVTGRNERRVATPGDASDPAWSPLIQ"
     gene            complement(4981..5700)
                     /locus_tag="VGC27_11015"
     CDS             complement(4981..5700)
                     /locus_tag="VGC27_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283140.1"
                     /translation="MGLMGSLVLHAGIIAVTFFTWSHKLDIMDQASPVVPVDLVTVGD
                     KTDIAPMVKEEPKPIEEQIQITAPPLENLQPPKFEVAPDVKPVPAPKPPPKREVFDIN
                     NIQAMLNKRQAAAPKNARVGPQNVKGIGEQSAMTADLKAMLQSEIFRCWSPPVGTPHP
                     ENLIVSFKLFLNRDGSIAQPPQLSADSAAAAAGDPYMRAAAEAARRAIYTCAPYRLPA
                     DRYNQWHDIDFTFYPRDMMGQ"
     gene            complement(5729..6175)
                     /gene="tolR"
                     /locus_tag="VGC27_11020"
     CDS             complement(5729..6175)
                     /gene="tolR"
                     /locus_tag="VGC27_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEY0283141.1"
                     /translation="MASVQMIERGRRSRRRRPISEINVTPFVDVMLVLLIVFMVTAPL
                     LTVGVPVDLPKTRAQALSQDREPLAVTIKSNGQIYLQNTPIAEDELVPKLAAIAANGY
                     DQRIFVRGDRSVDYGRVMEVMGMLSTAGFTHIGLVTDIVRPKPDEK"
     gene            complement(6175..7047)
                     /gene="tolQ"
                     /locus_tag="VGC27_11025"
     CDS             complement(6175..7047)
                     /gene="tolQ"
                     /locus_tag="VGC27_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEY0283142.1"
                     /translation="MNMKRRIAAVASALLIVAALAAPAMAQSGPTTAPTGPAAESAPL
                     AAPVTNVDTIQSGDSLEPSIGGFNVISLFMHADPVVKAAMLLLLAASLWSWAIIINKA
                     LALSNLRRRATKFEKLFWSGQSLDELFAQFASRNDHPFAAVFVSGVREWRRAFEHGAP
                     RESAVAGVKDRVEKAMSVTISREADAIESQIGFLATVANTAPFVGLFGTVWGIMNSFA
                     AIAARHDTTLAIVAPGIAEALFDTAMGLLAAIPAAIFYNRYVGEIGRYTNRLDAFADE
                     LSGILSRQLDEKAR"
     gene            complement(7148..7591)
                     /locus_tag="VGC27_11030"
     CDS             complement(7148..7591)
                     /locus_tag="VGC27_11030"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEY0283143.1"
                     /translation="MTDFQNPGRFEGKVYILPIRIYYEDTDLSGVVYHANYLRFMERG
                     RSEYFYALGVKVAGLDEPEPTAWTLRKVDLEYFRPARVGDLIEVHTRATALTGVRMSA
                     EQTIFCNGALLTRGHVEACIVTLTGKPRRIPQDIRDKLQPFLYET"
     gene            complement(7584..8399)
                     /gene="ruvB"
                     /locus_tag="VGC27_11035"
     CDS             complement(7584..8399)
                     /gene="ruvB"
                     /locus_tag="VGC27_11035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY0283144.1"
                     /translation="MAKRRSTSGPVLARAGDLAAILTNLEPRDVLFIDEIHRLNPAVE
                     EILYPAMEDFELDLVIGEGPSARTVKITLAPFTLIGATTRSGLITTPLRDRFGIPVRL
                     NFYSAEELHSIVERGARVLGFNLTSDGAREIAGRARGTPRIAGRLLKRVRDFASVAGH
                     ARVDAKIADAALTRLEVDGRGLDAFDTRYLRLIAENFGGGPVGIETIAAALGEARDAI
                     EEIIEPFLLQQGFVQRSPRGRILTGNAFAHLGLPSPPRDPAQAALFADEDGDD"
     gene            8410..8589
                     /locus_tag="VGC27_11040"
     CDS             8410..8589
                     /locus_tag="VGC27_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283145.1"
                     /translation="MLDDIVARQWGYLRAVNPLAHTTDTQLAATALAHGFTFATRNLK
                     HFRFDGLKLANPWEA"
BASE COUNT         1620 a         2759 c         2629 g         1618 t
ORIGIN      
        1 cccgccgtgt ccgcgatggc ggccgacggc gtcgagttca tcaataaaga tcagacaggg
       61 cgctgacttg cggccctgtt cgaacatgtc gcgcacgcgg ctggcgccca cgcccacgaa
      121 catctcgacg aaatcggaac cggagatggt gaagaaggga acattcgctt cgccggcgat
      181 ggcgcgcgcc agcaaggtct tgccggtgcc cggcggaccg accagcagca cgcctttggg
      241 aatgcgcccg cccaagcgct ggaatttctg cggatccttg aggaaatcga cgatctcctt
      301 gaggtcgtcc ttggcctcgt caaccccggc gacatcctcg aaggtgatcc ggccggtgcg
      361 ctcggtgagg agcttggcgc ggcttttgcc gaagcccatc gccttgccac caccggactg
      421 catctgcctg atgaagaata tccatactgc gatgatcagc agcatgggga accagttgat
      481 cagaatgttg acgatactga acgcaccgtc gtcggcctgc gcgacactta tatcgacttt
      541 atgcgacctc aatgtcgtcc aaagcgactg gtcgctcgtc ggtacggtgg tcgagaacgc
      601 ctgcccattc gtcatcgtcc caatgacctg gtcgcccttg acggtgacct tcttgacacc
      661 gccccgctcg acttcctgat tgaactgcgt gtaggagatg acagaggtgt tggtgtgctg
      721 agcagacccc tggaacagat tgaacaagag caccagcagc agtgccactg cgatccacag
      781 cgccagattg cgtaggttat tcaagggcgt cctttccgcg aactctcaag gcaacgattc
      841 ggccggtggc cttcttcata tatagatagg tcgcttcgcc cacattgcca ggaccggaaa
      901 cgttaaggaa tttgtgcggc aacggcaatc atattgcccg aatgacctta aaaaggtctt
      961 tggggccgtt agccgttgtt tttcatggtc ttgcggcggt ttccctcccg ccgcaccagc
     1021 aaagtgcccc ggccgaagca ggcgcgtgcg gacggcgccg gcccgatccg gcaaccatgc
     1081 agcgtgcgac ccgccccgat cgaaccgtcc gcaatccggg agaacagccg ttcgagccgt
     1141 tcgaagcgcg gccggtaggg attctccgaa acttccatca gcactgccgc cagggcccgc
     1201 aaacccaatt cgtgcggcgc gccggtcaag gcggcgggat caagtagcgc ctcggtgcgg
     1261 tcgaaacgac aggcccgcgc cagcacggcc tggctgacct tatcgagcgc cgcccgtgcc
     1321 cgcgccagat gggctgccgc atcggccacg cgctccttgc tgagacccat ggcttccagc
     1381 gccggccaag cgttgcgaat cctgacccgg gcgaaatgcg gatcggcgtt catcgggtcg
     1441 tcgagccaga cctgtttgcg cgcccgaagc tcatcccgca actcctcgcg gccaaaggcg
     1501 aggagcggcc gcaccagcat caatccggag aagcccttca cgggataagg cgcgaccatg
     1561 cgcatcgccg caagcccgtc cacgccgctg ccgcgcgcca gtctgagcaa gaacgtctcc
     1621 gcctgatcgt cgcgcgtatg gccgacataa atcgccttca gactattttt ctttgcccac
     1681 tcgcccatca atcgatagcg tgcctcgcgt gctgcggcct cgatatcgga gcgcggcgct
     1741 tcgcctttgc gcacgagaat tcgcgccggc aggcccgccg cctttgcctg gcgcagaacc
     1801 ttgcgcgctt cgccggccga ttccggccga agggaatgat cgacgcagag cataacgggc
     1861 ggcggcaagc ctgccctctt cgcccaatcg cgcagaagga acatcagcgc cagcgaatcg
     1921 ctgccgccgg atactgcgac ggcgcatggc caaggcgcgc ccgccaccgt caccgcatca
     1981 gcaaaacgcg ccgcaagctg cttgttattt gcaggatatc gcgcgcgcgc tggcggcttg
     2041 cgagaggacc gaagcggagg cattccggta tttgcccttg atcgagccaa gcgtcaggca
     2101 gccttctttt gtctggccca gcgcgagaag cgactggccg agcttcagca tgctatcggg
     2161 accgcgcgag cttgtggggt atttcttgat cacctcggcg aaggcctgcg cggcggcgcc
     2221 gtaatccttc tgaacatagg cgacgtctcc cacccagtag acggcctgcg gcgcaagcgc
     2281 gtccttggga ttgtcgtcgg cgaaagtgcg gaaagcggcg cgcgcctcgt cgtattgcgc
     2341 cctggcgaga aggttcatcg ccgcttcgaa ttggggacga gcatcgggca ctgcgttctg
     2401 cgacagcgtc ccgagcgtgc cgccggagcc gagcatcggc tggcctacag gcattgcacc
     2461 ggcgtccgcg cccagattac agagcagcga ctcgccaaat tgccccccct tgttgccggc
     2521 tcccagcaat tgggcggata aggtacagag cttgtaatcg taatccttct gctggctgtc
     2581 gagcttcgca cgcaactcct gcatgctata gctgacttgc tcgttctgcc cggtcagccg
     2641 ttgcagcgac tgttcgaggt cttgaacgcg ctgtttcaga tcggcgatct gggcgtcctg
     2701 attatcctcg tgctgctggc gggcggcttt ttcttcgtcg ctctcgccga acagaccggc
     2761 cagctgcacg ccgcgcattt gcgcgcgcgc cggcgcatcg gaaaccgttt gcgtcgctcc
     2821 ggcagtgccc gcgaacgccg aaaccagaac aagagccgca gccgcgcccg tcaatcgtcc
     2881 cgtcatggtc ctcaccggtc agaaagagtg ttcatgttac gaaacaagtt agccgaaaat
     2941 gcggcccgca gcacgttaag acaaaaaagg gcgccgatgc cggcgccctt tccgtaagct
     3001 ttgcgttgcc taggctcagg aattagcgcc agccgcgatt gccgtcacac cgcgacggtt
     3061 ttgcgcccag caggattccg tcgactccga acaaatcgga cgttccttgc cgtaggaaat
     3121 cgtctcgatg cgcgcagccg caacgcccag gctgatcaga tattccttca ccgcgttggc
     3181 gcggcgggcg cccagcgcca gattgtattc gcgcgtgccg cgttcgtcgg cattgccttc
     3241 gatggtgacg cggacggacg gatattgcgc aagccaggtc gcctgcttct gcaacgtccc
     3301 tttgtcgact tcgaggatgg cgtatttgtc atagtcgaaa tggaccgtat caccgacgtt
     3361 gacgcggaaa tcctcggcac tgcccggtat gaccgacggc gcgggggtga caacgggagc
     3421 cggagccggg gccggggccg gcggcggctt ggtttcgcaa cctgcgagca cgaaggcaaa
     3481 cgcaatggcg gccagtttca gaccggtaga gatattcttc atggcaatcc caactccagt
     3541 ggcacacgcc ctgttccaga tgcttcggga ccgcgtgtat tagtccctct ttcacacccc
     3601 cctaacgcca agtccacccg ggacgggcaa ctcggcgggc atgcattcta aggactaccc
     3661 gcctattgga tcaagggcga ccatgccgga tccgaggcat ctcctggcgt cgcaacacgc
     3721 cgttcgttgc gcccggtaac gtcaaccgac catatttgag agccgcggcc accctggatt
     3781 gtcctggtaa acatcagcac gcgcccgtta ggcgcccagg taggaccttc gtcctgccag
     3841 ccattcgaca acatgcgctc gccggtcccg tccggccgca tgacccaaat gcggaaactg
     3901 cccgcctgtt tggtgaaagc gatcagatcg ccgcgcgggc tccacaccgg cgtgccgttg
     3961 cgcccctgcc cgaaactgat tcgatgctgg ttggaaccgt ccgcgttcat gatatagatc
     4021 tgctgcgagc cgccgcggtc ggattcgaag gcgatctgct tgccgtccgg ggaataggac
     4081 ggcgaggtat cgatcccggg atcgtgagtc aggcgcgcaa ccgcgcgggt gcgcaaatcc
     4141 atcacataga tgtcggaatt gccgccttgc tcaagactca tcaccacctt gttgccgtcg
     4201 ggcgaaaagc gcggggaaaa cgtcatgttc gggaaatcgc cgagcttctc ctggcggccg
     4261 ctttccatat cgaacaggta tacgcgcggc ttgcccacga cgtacgacat gtaggcaatc
     4321 atctgcgccg tgggattgaa gcgcggcgtc aataccaggt agccgttggt gaggaagctt
     4381 ggattggccc cgtcctcgtc gatcaccgcc agacgcttct tgcggttcag cgcgggtccg
     4441 gattccgaaa tgaacacgat gcgcgtatcg aaataaccct tttccccggt gatacgttga
     4501 tagatcgcat cggaaatcat gtgagagatg cgccgccaat tggcgggttg ggtgaaatat
     4561 tgcagaccca gcatttggct tccgccatac acatcccaca ggcgaaaatc gacgcgcagc
     4621 ctgccgtccg gttgtaacgc cgccgaaccc gtcaccagcc cttgtgcggt gatgatgcgc
     4681 caattggcga aattgggcac gacgttgatg ttagtcacct tgtcgatgta ggatttggga
     4741 tcgagcggct tgaacaatcc cgaacgttcc agatcggcgc gcacgacttt ggcgatgtcc
     4801 tggctgaccg ccacgtcgcc cgacgagccc gccacgaaat cgggaatggc gatcggcagg
     4861 ggttggatat tgccctgatt gacgtcgacc ttcagcgccg caagggccgg cgaggacgcc
     4921 gcgagcgaca gcgccaccgc catggccaat ccgatcttcc tcattccctg tctcccattg
     4981 ctactgtccc atcatgtcac gcggatagaa ggtaaagtcg atatcgtgcc actggttgta
     5041 tcggtcggcg ggcaaccggt atggtgcgca ggtatagata gcacgccgcg ccgcttccgc
     5101 cgcggcgcgc atatacggat cgcccgccgc ggcagccgcc gagtctgccg acaattgcgg
     5161 cggttgtgcg atggaaccgt cgcggttcag aaataactta aaggacacaa tcaggttttc
     5221 agggtgcggc gtcccgacgg gcggactcca gcatcggaag atttcactct gcaacatagc
     5281 tttcagatcg gcggtcatcg cgctctgttc gccgataccc ttgacgttct gggggccgac
     5341 tctggcgttc ttcggtgccg ccgcctggcg cttgttgagc atcgcctgga tgttgttgat
     5401 gtcaaacact tcccgcttcg gcggcggctt cggcgcgggc accggcttca cgtccggcgc
     5461 aacctcgaac ttcggcggct gcaggttttc taaggggggc gccgtaatct gtatctgttc
     5521 ctcgataggt ttcggttcct ccttcaccat gggcgcaatg tcggtcttgt cgccgacggt
     5581 gacgagatcg accggcacga cgggcgacgc ctggtccatg atgtcaagct tgtgtgacca
     5641 ggtgaagaac gtcaccgcga tgataccggc atgcagcacc agcgagccca tcagccccaa
     5701 gcgcggcgct tgcctgtctg ctgtagcgct atttctcatc tggcttagga cggacaatgt
     5761 cggtgacgag tccgatatgc gtgaaccccg ccgtgctcaa catccccatc acttccatca
     5821 cccggccgta atcgaccgac ctgtcgccgc gcacgaagat gcgctggtcg tagccgttgg
     5881 cggcgatggc cgcgagtttg ggcaccagtt cgtcctcggc gatgggcgta ttttgcagat
     5941 agatctgccc gttgctcttg atcgtcaccg ccaaaggttc gcgatcctgg ctcagcgcct
     6001 gggcgcgggt cttgggcagg tccaccggca caccgacggt caagagcggc gcggtcacca
     6061 taaacacgat aagcagcacc agcatcacat cgacgaacgg cgtgacgttg atctccgaaa
     6121 tcggacggcg ccgcctggag cgccggcccc gctcgatcat ctgaacgctg gccatcagcg
     6181 cgccttttcg tcgagctggc gcgacagaat ccccgacaat tcgtcggcga acgcgtcgag
     6241 ccggttggta tagcggccga tctcgccgac ataccggttg tagaagatcg ccgccggaat
     6301 cgcggccaga aggcccatcg cggtgtcgaa cagggcctcg gcgatgcccg gcgcgacgat
     6361 ggcgagcgtg gtgtcgtgac gcgcggcgat ggccgcgaag gaattcatga tcccccacac
     6421 cgttccgaac aggccgacga agggcgcggt gttggcgacc gtcgcaagga aaccgatctg
     6481 gctttcgatg gcgtcggctt cgcgcgagat ggtcacgctc atcgccttct cgacccggtc
     6541 cttgacgccg gcgacggcgc tttcgcgcgg cgctccatgc tcgaaggccc ggcgccattc
     6601 gcgcacgccc gacacgaaca ccgcggcgaa gggatgatcg ttcctcgagg cgaactgcgc
     6661 gaacagctcg tcgagcgact ggccggacca gaacaatttc tcgaacttgg tggcgcggcg
     6721 gcgcaggttc gaaagcgcca gcgccttgtt gatgatgatc gcccacgacc aaaggctggc
     6781 cgccagcagc agcagcatcg ccgccttcac caccggatcg gcatgcatga acagcgaaat
     6841 cacgttgaac ccgccgatgg agggttcgag cgaatccccg ctctgaatag tgtcgacatt
     6901 cgtcaccggc gccgccagcg gcgcgctttc cgcggccggt ccggtggggg ccgtagtcgg
     6961 tccgctttga gccatggctg gtgcggccaa ggccgcgacg atcaacaacg ccgaagcaac
     7021 cgcggcgata cgccttttca tgttcatgta cgcaacctca ttcccggccc aacgggccgg
     7081 attatgccga gtgggaatga aaatactcac ttaatattgg ccgtattttg gcggccaatt
     7141 gttaagccta tgtttcatat aggaaaggct gaagtttgtc gcggatatcc tgggggatgc
     7201 gccgcggttt gccggtcagc gtcacgatac aggcctcgac atggccgcgc gtcagcaagg
     7261 caccgttgca aaaaatcgtc tgttcggccg acatccgcac tcccgtaaga gccgtggccc
     7321 tggtgtggac ttcgatgaga tcaccgaccc gcgccgggcg gaaatattcc agatccacct
     7381 tgcgcaaggt ccaggccgtg ggctccggct cgtcaagacc cgcgaccttg acgcccaatg
     7441 catagaaata ttccgagcgg ccgcgctcca tgaatcgcaa gtaattcgca tgatagacga
     7501 cgcccgaaag gtcggtgtcc tcgtaataga tgcgaatggg cagaatgtag accttgccct
     7561 cgaaacgccc gggattctga aaatcagtca tcgccgtcct cgtccgcgaa cagcgcggcc
     7621 tgtgccggat cgcgcggcgg cgacggcagg cccagatgcg caaaagcgtt gccggtgaga
     7681 atgcgtccgc gcgggctgcg ctgcacgaag ccttgttgca ggaggaacgg ctcgatgatc
     7741 tcctcgatgg cgtcgcgcgc ttcgccgagc gccgccgcaa tagtttcgat ccccaccggc
     7801 ccgccgccga aattctccgc gatcaggcga aggtagcgcg tgtcgaaggc atccagcccg
     7861 cgcccgtcca cttccagccg cgtgagcgcg gcatcggcga tcttcgcatc gacgcgggcg
     7921 tgtccggcga cggaagcgaa atcgcgcacc cgcttcagca ggcgcccggc gatgcgcggc
     7981 gtgccccgcg cccgtccggc gatctcgcgc gcgccgtcgg aggtcagatt gaaacccagc
     8041 acgcgcgcgc cgcgctccac gatcgagtgc agttcctccg ccgaatagaa attcagccgc
     8101 accggaatgc cgaaacggtc gcgcaacggc gtggtgatga ggcccgaacg cgtcgtggcg
     8161 ccgatcagcg tgaagggggc cagcgtgatc ttcaccgtgc gcgcggaagg cccttcgccg
     8221 atcaccagat cgagttcgaa atcctccatc gccggataaa ggatttcctc caccgccgga
     8281 ttgaggcgat ggatttcgtc gatgaacagc acgtcgcgcg gttcgagatt ggtgaggatg
     8341 gcggcgagat cgcccgcccg cgccagcacc gggccggagg tcgagcgacg cttcgccaac
     8401 cgtatcgtcg tgctggacga cattgtcgcc cgccagtggg gctatctgcg tgcggttaat
     8461 cccttggcgc atacgaccga cacgcagctt gcagccacgg cgctcgccca cggctttacg
     8521 tttgccacgc gcaacctgaa gcatttccgc ttcgacggtc tcaagctcgc caatccgtgg
     8581 gaggcatgat ggcggtcaag ccgcagcctt atcgactgat gacgcc
//
LOCUS       DASWPU010000521         6201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_90076,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000521 DASWPU010000000
VERSION     DASWPU010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90076"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VGC27_11045"
     CDS             complement(<1..323)
                     /locus_tag="VGC27_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283146.1"
                     /translation="MAKTSACRVAAALLSLAAVALFPAGCGKPQDAVTKPIPEIVQKN
                     GRFALMVDGAPFLMLGAQANNGSNYPAMLPKVWPVLDKLHANTLEIPVAWEQIEPEEG
                     RFDFS"
     gene            complement(462..2132)
                     /locus_tag="VGC27_11050"
     CDS             complement(462..2132)
                     /locus_tag="VGC27_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY0283147.1"
                     /translation="MDHRGDNPAADARFADGRALHRQGRLAEAETQYRAALASDNLHS
                     DALEWLGVLCLQTGRIDEAVRLLGLAAERHPEKAAYHDNLAAALLQAQRPQEAAAAWR
                     RSLDVVPDAIGTRVNLGTLLGRLGRSGEAMVHFAHAVAADPHLKAVCREPKALSSEEE
                     RCRTVLENCRHILARYPNYAPAQYSMACSLLNLGRSAEVRQACERAIALDPTVPVYYH
                     ILVHGGDAEQKAAAVAALERLAGQEAAFPEDGRAMLHFLLARAYDDQRRYGEAFAHLQ
                     HANAIKRGRVVYDEARELDRLSAIAAAFPSARLQADGSGATSDAPVFVLGMPRSGTSL
                     IEQILACHPDVYGAGERTLLGDLVAGDHDAVPAADMHDVEDWRRLGEAYAHQLARIGA
                     GAKRVVDKQPFNFKLIGCIRLALPQARIIHVRRDPRDTCFSCYGLMFAGEIGFAYDLA
                     ELGRYYRAYEALMAHWRAALPEGVMLEVRYEDLVDDLESQARRMLAYCGLEWDVRCLD
                     FHKAERAVATASLMQIRQPLYRSAIGRWRPYEMHLAPLIEALHAEHSA"
     gene            complement(2271..5363)
                     /locus_tag="VGC27_11055"
     CDS             complement(2271..5363)
                     /locus_tag="VGC27_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0283148.1"
                     /translation="MSNKKVLLGSVSAVAITVLIGPALAQDAIETVIVTGTRASLQSA
                     QTIKQEADQMVDSITAVDIGALPDRNVAEALQRVPGVTLQRNDAPNDLTRMGSTGNSV
                     FVRGLSWVKTLVNGRDEFTAVDGRSLSFADVSADLMAGVDVYKNPSAKMIEGGVGGTV
                     DLRTRHPFDQDGQVIAFSGDYTYGDLVDRALPSANALYSNRWETGIGEFGILASVDWQ
                     NQITRTEGINLTEYDCWDVVAGSYNKITDPTYAACAALPTGVTGKLTAPNGWAWRQLE
                     FKQQRLASNLVVQWRPNDKWELTFNALNSYAHVLDMEHYAFNPLTATQVAAGNYDAEG
                     RWLGGPSSTSSIDTRAGTGHNRNTDLSLDIKFAPTDALEISANVQFVESSSPYRNVTM
                     FTGFNGAAPTFDIDVTGETPTIGWTDNSGGRLQDPASYFWLAAMDHLQYNSAHSTNAR
                     LDASYKFSGHGIFSLFRSVDAGFRTEQKIAVSRSTGYNWGATCPTGWGDPWSGCPLLD
                     GTVASPYTAIDGGTVSSGSDAVSNLNGYAELFTYHPVFGNSVPSFWLPKASLAAMNTV
                     DSYKLFAAVEPADAEAWFGVQNWSNWHSYASIAGCTGPDTTCIEAYKNLTGGASSGNR
                     ISTQREETYAGYLQVNYGLETFLGYDIPIDGNFGLRLVRTNDVVSDGKLVMPNMNQNS
                     CVIGTVDANGNVTTDCSGFLAGIDFLGGLSGQGALVDRPGVSSGYTNYLPSFNFRARL
                     SDELQARLAYSESLVRPDFAYTNSSATLQYNFYDANTYQSGIFRSNPSGYGGNPYLKP
                     MRARNYDAGLEYYFAPTGSLTFSVFHKDLSDYIFTSTTRTAFIHPISGNTLDFDYTTY
                     VNGEKGKVEGFELAYTQFYDSLPGWLSGFGVQANYTKIYNSGGHNGAADLTSTVAIAN
                     SGDQSLPLEGMSNDSYNLALMYEKYGIDARLAWNWRSTYLSSSSDSNTKLPVWLENYG
                     QLDGSAFYSFWDHYKIGVQVTNILGASFYTDMGYSDYHPRTNWIKNDRKFAVIFRTSW
                     "
     gene            complement(5813..>6201)
                     /locus_tag="VGC27_11060"
     CDS             complement(5813..>6201)
                     /locus_tag="VGC27_11060"
                     /inference="COORDINATES: protein motif:HMM:NF045341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283149.1"
                     /translation="STLPNLWDTHPPFQIDGNFGASAGIAEMLLQSQDDMLRLLPALP
                     GAWPDGDVRGLKARGDATVDLAWRGGKAVRIAVTAGKDGPVCLASPLFAASYAADDRR
                     RPAEREGRLCFVAAKGERYHFAAATR"
BASE COUNT         1152 a         1903 c         1980 g         1166 t
ORIGIN      
        1 taagagaaat cgaaccggcc ttcctcgggc tcgatctgct cccacgccac cgggatttcc
       61 agcgtgttgg cgtggagctt gtcgagcacc ggccagacct tcggcagcat cgccggatag
      121 ttcgagccgt tgttggcctg cgcgcccagc atcaggaaag gcgcgccatc gaccatcagc
      181 gcgaagcggc cgttcttctg cacgatctcc gggatcggct tggtcaccgc atcctgcggt
      241 ttgccgcagc cggcaggaaa caaggcgacg gccgcaagcg acagcaatgc cgcagcaacc
      301 cggcaggcgc tggtcttcgc catgacgtca tcctccctgg tcttatggct gccccgcagc
      361 ggagcgccga cctttaatat gatttattat tggggcggac tttgaactga aaatgccgat
      421 atttcaactg gatttgcgta tgcctgggga gacaatccgc ctcaagccga atgctccgca
      481 tgcagcgcct ctatcaacgg agcgagatgc atttcatagg gccgccagcg gccgatggcg
      541 ctgcgataca agggctggcg aatctgcatg aggctggcgg tggcaacggc ccgttcggcc
      601 ttatggaaat cgaggcagcg cacatcccat tccaggccgc aataggcgag catccgcctg
      661 gcctggcttt ccagatcgtc caccagatct tcgtagcgca cctccagcat caccccctcg
      721 ggcaaggcgg cgcgccaatg cgccatcagc gcttcgtagg cgcggtaata gcggccgagt
      781 tccgctaggt cgtaggcgaa accgatctcg ccggcaaaca tcaggccgta gcaggaaaag
      841 caggtgtcgc gcggatcgcg ccgcacatgg atgatacggg cctgcggcag tgccaggcgg
      901 atgcacccga tcagcttgaa attgaagggc tgcttatcga cgacgcgttt ggcccccgcc
      961 ccgatgcgtg caagctgatg cgcataagcc tcgcccagcc ggcgccaatc ctccacatcg
     1021 tgcatgtctg ctgccggcac ggcgtcatgg tccccggcga cgagatcgcc cagcaaggtg
     1081 cgctcgccgg caccataaac gtctgggtgg caggccagaa tctgttcgat caggctcgtc
     1141 cccgagcgcg gcatgccaag aacgaacacc ggcgcatcgc tggttgcgcc gcttccatcc
     1201 gcctgcaacc gcgcggacgg aaaggcggcg gcgatggccg acagccggtc gagctcgcgc
     1261 gcttcgtcat agacgacgcg gccccgcttg atggcgttgg cgtgctgcag atgggcgaag
     1321 gcttcgccgt agcgccgctg atcgtcatag gccctggcaa gcaggaaatg cagcatggcc
     1381 cggccgtctt cgggaaacgc cgcttcctgc ccggccaggc gctcgagcgc cgcaacggcg
     1441 gcggctttct gttccgcatc gccgccgtga acgagaatat gataataaac cggcacggtg
     1501 ggatcgagcg cgatggcccg ctcgcacgcc tgacgcacct ctgccgaacg ccccaggttc
     1561 agcaggctgc acgccatgct gtactgggcg ggggcatagt tcggataacg ggcgaggatg
     1621 tgccggcagt tctccagcac cgtccggcag cgttcttctt cgctcgacag cgctttgggc
     1681 tcgcggcaaa cggctttcag atgcggatcg gcggcgacgg catgggcaaa atgaaccatc
     1741 gcttcgccgc tgcgcccaag ccggcctagc agggtgccga gattgacccg cgtgccgatg
     1801 gcatccggca caacgtccag cgaccgccgc cacgccgccg ccgcttcttg cggcctttgc
     1861 gcctgcagca gcgcggcggc gagattgtcg tgataggccg ccttttcggg atgccgttcc
     1921 gccgccaggc cgagcaggcg gacggcctcg tcaattctcc cggtttgcag gcataagacg
     1981 cccagccatt cgagggcatc gctatgaaga ttgtccgagg cgagagcggc acgatactgc
     2041 gtttccgctt ccgcaaggcg accctggcgg tgcagcgccc gcccgtcggc gaaacgcgca
     2101 tcggccgccg gattgtcgcc gcggtggtcc atggaatctc cctgtgcccc ggtggcgctg
     2161 acgagatgca agtttcgcca cgcggccccg cgaatgcaag cgcgcgacgc ggcggcaaaa
     2221 aatgccccct cggcttgcgg ccgagggggc ggtcaaacgt ccatgctcgc ttaccagctg
     2281 gtgcggaaga tcacagcgaa cttgcggtcg ttcttgatcc agttggtccg cgggtgatag
     2341 tccgaatacc ccatatcggt atagaagctg gcacccagaa tgttggtgac ctggacgccg
     2401 atcttatagt ggtcccagaa gctgtagaag gccgagccgt cgagctggcc gtagttttcc
     2461 agccacaccg gcagcttggt attggagtcg gagctcgagg acagataggt cgaacgccag
     2521 ttccaggcca agcgcgcgtc gatgccgtac ttttcgtaca tcagggcgag gttgtagctg
     2581 tcgttcgaca tgccttcgag cggcaggctc tggtcgccgg aattggcgat ggcgacggtg
     2641 ctggtcagat cggcggcgcc gttgtggccg ccggaattgt agatcttggt gtaattcgcc
     2701 tgcacgccga acccgctcag ccagccgggc agcgaatcgt agaactgggt gtaagccagt
     2761 tcgaatcctt cgaccttgcc cttctcgccg ttgacatagg tggtgtagtc gaagtcgagc
     2821 gtgttgcccg aaatcggatg gatgaacgcc gtccgcgtcg tcgacgtgaa gatgtagtcc
     2881 gacaggtcct tgtggaacac cgaaaaagtg aggctgccgg tcggcgcgaa gtaatattcg
     2941 aggccggcat catagttcct ggcccgcatc ggcttgaggt acgggttgcc gccatagccc
     3001 gaggggttcg acctgaagat gcccgattga taggtattcg cgtcgtagaa attatactgc
     3061 agcgtcgcgc tgctgttggt gtaggcgaag tccgggcgaa caagggactc cgaataggcc
     3121 aggcgcgcct gcagctcgtc gctcaaccgg gcgcggaagt tgaagcttgg caggtagttg
     3181 gtgtagccgc tcgacacgcc gggacgatcg accagcgcgc cctgcccgct gagcccgccg
     3241 aggaaatcga tgccggccag gaagccgctg caatcggtgg tgacgttgcc gttggcatcg
     3301 accgtgccga tgacgcagct attctggttc atgttgggca tgaccagctt gccgtcgctg
     3361 acgacgtcgt tggtacggac caggcgcagg ccgaaattgc cgtcgatcgg aatgtcgtac
     3421 cccaggaagg tttccaagcc ataattgacc tggaggtaac cggcataggt ctcttcccgc
     3481 tgggtgctga tccggttgcc cgaactggcg ccgccggtga ggttcttata ggcctcgatg
     3541 caggtggtat cggggccggt acaaccggcg atcgaggcat aggagtgcca gttggaccag
     3601 ttctgcacgc cgaaccaggc ctctgcgtcg gccggctcga ccgccgcaaa cagcttgtag
     3661 gaatcgacgg tgttcatcgc cgccagcgag gccttgggca gccagaagct cggcacgctg
     3721 ttgccgaaga ccgggtgata ggtgaacagc tcggcatagc cgttgaggtt ggacacggca
     3781 tcggagccgg acgaaaccgt gccgccgtct atcgcggtat agggactggc gaccgtgccg
     3841 tcgagcagcg ggcagccgct ccacggatcg ccccagcccg tcgggcaggt ggcgccccag
     3901 ttgtagccgg tcgaacgcga caccgcgatc ttctgctcgg tgcggaagcc ggcatccacg
     3961 cttctgaaga ggctgaagat gccgtggccg ctgaacttat agctggcatc gaggcgggcg
     4021 ttggtggagt gcgccgagtt gtactgcagg tggtccatcg cggccagcca gaagtagctc
     4081 gccggatcct gaagcctgcc gccgctgttg tcggtccagc cgatcgtcgg ggtctcgccc
     4141 gtcacgtcga tgtcgaaggt cggggccgcg ccgttgaagc ccgtgaacat cgtcacgttg
     4201 cgatagggcg agctgctctc gacgaattgg acgttggcgg agatttccaa cgcatcggtc
     4261 ggggcgaact tgatgtcgag gctgagatcg gtattgcggt tatgcccggt gccggcgcgg
     4321 gtatcgatcg acgaagtcga agacggcccg ccgagccacc tgccttcggc gtcgtaattg
     4381 ccggccgcca cctgcgtcgc ggtcagcggg ttgaaggcat aatgctccat gtcgaggaca
     4441 tgggcgtaag agttcagcgc gttgaaggtc agctcccact tgtcgttcgg acgccactgg
     4501 acgacgaggt tggaggcgag gcgctgctgc ttgaattcca actggcgcca ggcccagccg
     4561 ttcggcgccg tgagcttgcc ggtgacgccg gtgggcagcg ccgcacaagc ggcgtaggtc
     4621 ggatccgtga tcttgttata gctgccggcg accacgtccc agcaatcgta ttccgtcagg
     4681 ttaatgcctt cggtgcgggt gatctggttc tgccagtcga ccgacgccag gatgccgaac
     4741 tcgccgatcc cggtttccca gcggttgctg tacagcgcat tggcggaagg cagggcgcga
     4801 tcgaccagat cgccgtaggt atagtcgccc gagaaggcga ttacctgtcc gtcctggtcg
     4861 aaggggtggc gggttctgag atcgaccgtg ccgccgacgc cgccttcgat catcttggcg
     4921 ctggggttct tgtagacatc gacgcccgcc atcagatcgg ccgacacgtc ggcgaaactc
     4981 aaggagcggc cgtcgacagc ggtgaattcg tcgcggccgt tgaccagcgt cttcacccac
     5041 gacagaccgc ggacgaacac cgagttgccg gtgctgccca tgcgggtgag atcgttcggg
     5101 gcgtcgttgc gttgcagcgt cacgcccgga acgcgttgca gcgcttcggc gacgttgcga
     5161 tccggcaagg cgccgatatc gaccgccgtg atcgaatcga ccatctgatc ggcttcttgc
     5221 ttgatcgttt gcgccgactg caagctggcc cgagtgccgg tcacgatgac cgtctcgatg
     5281 gcatcctgcg ccagcgccgg tccgataagg acggtgatcg ccacggccga cacgctgccc
     5341 agcagcactt ttttgttgct catctttttc acggagtccc tcccaaaacg attcagcctt
     5401 gatctgccgg atgattaatg atggtataga tcatataaat catccggtga tacgaacagc
     5461 gaatcacgcc tggaggccca gcaatgaaat aatcaatcta agaaaagtac ggccaaaaac
     5521 cgacgattcc ttcctcgatt atttcgcttg gcaaaggcca gaagcgtatt cactccctgt
     5581 gttcagaact ttcctgacat tctatttcct tatgactttc aaggacatca tggaagttcc
     5641 acttaataat attactatta tcataagtcc tacgctgtca ccagacgacg acacatggcg
     5701 caaccaaaaa aacatcgcca tgcctcataa ttgtcacggt ttgaacaagg ttgccttgat
     5761 tgaaggccgg cacaaacatg cggcagcggc gaaacacgct gcgcccgacg attcatcgtg
     5821 tcgcggcggc gaaatggtag cgctctcctt tggcggcgac gaaacacaac cgcccctccc
     5881 gctccgccgg ccggcggcga tcgtctgcgg cataggacgc ggcgaacagc ggcgaggcga
     5941 gacagaccgg cccgtctttg cccgccgtca ccgcgatgcg gaccgccttg ccgccccgcc
     6001 atgcgagatc gaccgtcgcg tcgccgcgcg ccttgaggcc gcgcacatcg ccgtcaggcc
     6061 aagcccccgg caatgccggc aggaggcgca gcatgtcgtc ctggctttgc agcagcatct
     6121 cggcgatgcc ggcgctggcg ccgaaattgc cgtcgatctg gaaaggcgga tgcgtgtccc
     6181 acagattggg cagggtcgat t
//
LOCUS       DASWPU010000522         5943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_47487,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000522 DASWPU010000000
VERSION     DASWPU010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47487"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="VGC27_11065"
     CDS             <1..1178
                     /locus_tag="VGC27_11065"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY0283150.1"
                     /translation="DESLRADLAAMERAGTLKSYRHITGPMDTEVTMAEKSGRVLVLS
                     SNNYLGLADHPEVVAAGKKALDEYGAGTASVRFICGTFSIHDEIEKALAKLSHSESAL
                     TYVSCWAANTGLIPAVAGEGDALVSDALNHASLIDGCRGSKAKRLVYKHSDMGDLAAK
                     LAEVKGARRIFVVTDGVFSMEGDIARLPDIIAIARKAGAAIILDDSHGTGVMGKHGRG
                     TAEHFGVEGQVDIVTGTLGKALGGAAGGYVAGSQTLIDFLQQVSRPQLFSNALPATIA
                     ASAMKAIEILQREPGRVERLHAITRKMRDGLKARGFKPLDGESAIVPIIVGDTAFAIR
                     MSKELLAEGIFITGFGYPVVPEGTARLRIQACAALTDAQIDFALATFEKVGKTLGVI"
     gene            1347..2141
                     /locus_tag="VGC27_11070"
     CDS             1347..2141
                     /locus_tag="VGC27_11070"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY0283151.1"
                     /translation="MVQFFLPKGSSPKKGKVWPVPVTANGKKAKRTKEFRVYRYDPVE
                     GGNPRIDTYTVDLTDCGPMVLDALIKIKNEIDPTLSFRRSCREGVCGSCAMNIAGSNT
                     LACTKEITDVSGPVTVYPLPHMPVVKDLIPDMTHFYAQYASIEPYLQTRTAPPESEWK
                     QSPEDRAKLDGLYECILCACCSTSCPSYWWNSDRYLGPAALLQSYRWLADSRDEMTGE
                     RLDNLEDPFRLYRCHTIMNCASACPKSLNPAKAIGEIKQMLVKRSV"
     gene            complement(2341..3198)
                     /locus_tag="VGC27_11075"
     CDS             complement(2341..3198)
                     /locus_tag="VGC27_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0283152.1"
                     /translation="MADGKVYAKGWHLDEIDWTKFAPSKVEPWLLTAIKAAALVEFNA
                     PDYVTYLKRIFKDSGPDMFAAIEEWGREESQHGRTLGRWAEMADPAFKLDEAFARFRK
                     GYQPAHFVHPDEVSVRGSRRGEMIARCVVESGTSSYYTAVRDATEEPLLKEIAGRIAA
                     DEFRHYKLFYDTLQQQEERELPFWKKLSIAAGRITESDDDELCYAYYCATVPAGMEAA
                     TPYVRAPYAKAGYNTTLRLYRRRHISKLTQMVAKAVGMNPQGILTRFAELLVWRMLRV
                     RAGLSTELT"
     gene            complement(3348..3737)
                     /locus_tag="VGC27_11080"
     CDS             complement(3348..3737)
                     /locus_tag="VGC27_11080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF001851.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY0283153.1"
                     /translation="MTDKISFDLVSPERLLLSTEAEMVTIPGSEGDMGVMAGHMPLIS
                     TLRPGVITVSEGDQRFYVLGGFAEVNPGKLTVLAEEAVPVAELDAATLDLRIKNAEED
                     VALAKSDGERAKAQETLDYLKGLRAAL"
     gene            complement(3741..5183)
                     /gene="atpD"
                     /locus_tag="VGC27_11085"
     CDS             complement(3741..5183)
                     /gene="atpD"
                     /locus_tag="VGC27_11085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY0283154.1"
                     /translation="MNAVITSKLGRVTQVTGAVVDVQFDGHLPQIQNALETDNHGRRL
                     VLEVAQHLGESTVRTIAMDSTDGLVRGQQVTDTGLAIAVPVGEGTLGRIMNVIGEPVD
                     EAGPIQGAETRPIHAEAPSFVDQSTESQVLVTGIKVIDLLAPYARGGKIGLFGGAGVG
                     KTVIIQELINNIAKKHGGYSVFAGVGERTREGNDLYHEMIESGVNKDPKKGSTEGSKC
                     SLVFGQMNEPPGARARVGLTGLTVAEYFRDQGQDVLFFVDNIFRFTQAGSEVSALLGR
                     IPSAVGYQPTLATDMGELQERITTTNKGSITSVQAIYVPADDLTDPAPATSFAHLDAT
                     TVLSHAISEKGIYPAVDPLDSTSRILDARVIGQEHYDVARQVQQTLQRYKSLQDIIAI
                     LGMDELSEDDKIAVARARKLERFLSQPFHVAEVFTGFPGQFVELKDTIRSFKAVVNGE
                     YDHLPEQAFFMVGTIEDAVAKAQKLAAEAA"
     gene            complement(5265..5669)
                     /locus_tag="VGC27_11090"
     CDS             complement(5265..5669)
                     /locus_tag="VGC27_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEY0283155.1"
                     /translation="MAYTTKATSKGGREGRASLDGGALALSMSFPKELGGAGDGHNPE
                     QLFAIGYAACFNQAVIALGRKHGIDPAKAVVGVQVTLDKDEISFALKVDITLSVPGAD
                     KAAVKALLDDAHTICPYSRATRGNVPVTLTVV"
     gene            complement(5686..>5943)
                     /locus_tag="VGC27_11095"
     CDS             complement(5686..>5943)
                     /locus_tag="VGC27_11095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY0283156.1"
                     /translation="ATVYEYEPDEIEILADLLPRNVNVQIFRALLENVAGEFGAKMTA
                     MDGATRNAGEVIDKLTLLYNRTRQAQITKELIEIISGAEAL"
BASE COUNT         1056 a         1877 c         1857 g         1153 t
ORIGIN      
        1 tcgacgaaag cctgcgcgcc gatctcgcgg ccatggagcg cgcgggcacg ctgaaaagtt
       61 accgccacat caccggcccg atggacaccg aagtgacgat ggccgagaaa tccggccggg
      121 tgctggtgct ttcctccaac aactatctcg gcctggccga tcatcccgaa gtcgtcgccg
      181 cgggcaagaa ggcgctggac gaatacggcg cgggcaccgc ctcggtacgc ttcatctgcg
      241 gcaccttctc catccacgac gagatcgaga aagccctcgc caagctgtcg cacagcgaaa
      301 gcgcgctcac ctatgtctcc tgctgggcgg ccaatacggg gctgattccg gcggtcgcgg
      361 gcgaggggga tgctctggtg tcggatgcgc tcaaccacgc ttcgctgatc gacggctgcc
      421 gcggctccaa ggccaagcgg ctggtctata aacactccga catgggcgac ctcgcagcca
      481 agctcgccga ggtgaagggc gcgcggcgca ttttcgtcgt caccgacggc gtcttcagca
      541 tggaaggcga catcgcgcgc ctgccggaca tcatcgccat cgcgcgcaaa gcgggcgccg
      601 cgatcatcct cgacgattcg cacggcaccg gcgtcatggg caagcacggg cgcggcacgg
      661 ccgagcattt cggcgtcgaa ggccaggtcg acatcgtcac cggcacgctg ggcaaggcgc
      721 tcggcggcgc ggcgggcggc tacgtcgcgg gctcgcaaac gctgatcgat ttcctgcaac
      781 aggtttcgcg tccgcaattg ttctccaacg ccttgccggc caccatcgcc gccagcgcca
      841 tgaaggcgat cgagattttg cagcgcgagc cgggccgcgt cgagcgcctg catgcgatca
      901 cccggaaaat gcgcgacggg ctgaaagcgc gcggcttcaa gccgctcgac ggcgagtccg
      961 ccatcgtgcc gatcatcgtc ggcgacacgg ccttcgccat ccgcatgagc aaggaactgc
     1021 tggcggaagg tatcttcatc accggcttcg gttatcccgt ggtgccggaa ggcaccgcgc
     1081 gcctgcgcat ccaggcctgt gccgcgctga ccgatgcaca gatcgatttc gcgctcgcga
     1141 ccttcgagaa ggtcgggaag acgttgggcg tgatttgaag atgttgatct cttgtgcttc
     1201 gacaggctca gagggattgt ccgttccctt caccctgagc ttgccgaagg gtgacggaaa
     1261 gcaaggttag cggtccaaag cgcgttgcgg ggcggggcgt gctttgctaa gccagttggc
     1321 ggcacgccaa ccttccgggg agtcgcatgg tccagttctt tttgcccaaa ggcagcagcc
     1381 ccaagaaggg caaagtgtgg cccgtgccgg tcacggcaaa cggcaaaaaa gccaaacgga
     1441 ccaaggagtt ccgggtgtat cgttacgatc cggttgaagg cggaaatccg cgcatcgata
     1501 cctacacggt cgatctgacg gattgcgggc cgatggtcct ggacgccctc atcaagatca
     1561 agaacgagat cgacccgacg ctgagcttcc gccgctcttg ccgcgaaggg gtgtgcggct
     1621 cctgcgccat gaatattgcc ggcagcaaca cgctggcctg caccaaggag atcaccgacg
     1681 tttccgggcc ggtcaccgtc tatcccttgc cgcacatgcc ggtggtgaag gacctgatcc
     1741 cggacatgac gcatttctac gcccaatacg cctccatcga gccttatctg caaaccagga
     1801 cggcgccgcc ggagagcgag tggaagcaat cgccggaaga ccgcgccaag ctggacgggc
     1861 tttacgagtg catcctgtgc gcctgctgct cgacctcgtg cccgagctat tggtggaatt
     1921 ccgaccgcta tctgggtccg gcggcgctgc tgcaatccta tcgctggctg gccgacagcc
     1981 gcgacgagat gacgggcgag cggctggaca atctggaaga tccgttccgg ctctaccgct
     2041 gccatacgat catgaattgc gccagcgctt gcccgaagag cctgaacccc gccaaggcca
     2101 tcggcgagat caagcagatg ctggtgaagc gcagcgtgta gagtatttca ccgtttcacg
     2161 gaaacgatga aatactctag attccttgat tttcgcgcaa tctgacggca aaccgcgtcg
     2221 gcacgctgct gctaccgacc actttgccgg attgcgctct agcgctatgc ttcgcgtatg
     2281 tcgcgcttga atctcgccct gctcgccggc gtcgtggtcg ttctgctcct attagcggcc
     2341 ctaggtcagt tccgttgaga ggccggcgcg gaccctgagc atccgccaaa ccagcaactc
     2401 ggcgaacctc gtcagaattc cttgcggatt catgccgacg gccttcgcca ccatctgcgt
     2461 cagtttcgag atgtggcggc ggcgatagag gcgcagcgtc gtgttgtatc cggccttggc
     2521 gtatggcgcg cgaacatagg gcgtggccgc ttccatgccg gcaggaaccg tcgcgcagta
     2581 ataggcgtag caaagttcgt cgtcgtcgga ttcggtgatg cggcccgccg cgatcgacag
     2641 cttcttccag aagggcagct cgcgctcttc ctgctgctgc aacgtgtcgt agaacagctt
     2701 gtaatgcctg aattcgtcgg cggcgatgcg tccggcgatt tctttcagga gtggttcctc
     2761 ggtggcgtcc ctgacggccg tataatagga ggatgtaccg ctttccacga cgcagcgcgc
     2821 gatcatctcg ccgcgccgcg aaccgcgcac cgacacctcg tccgggtgaa cgaaatgcgc
     2881 cggttgataa cccttgcgaa accgcgcgaa ggcctcgtcg agcttgaaag cggggtccgc
     2941 catttcggcc caacggccta gggtcctgcc gtgctggctt tcctcgcggc cccactcctc
     3001 gatcgcggcg aacatgtccg gcccggaatc cttgaagatg cgcttgaggt aagtgacgta
     3061 gtcgggggcg ttgaattcga cgagggcggc ggccttgatg gcggtcagca gccaaggctc
     3121 gaccttggac ggggcgaact tcgtccaatc gatttcgtcc aggtgccatc ccttggcgta
     3181 gactttgccg tcagccatcg tccgatccat cagttgcggt attgcccatt ataccaaaca
     3241 gttgtgccgc ggcggaacct ccgcgcggcc cttccgctac ggaaaggtcc ataccatacg
     3301 ctgatggaat aatacggcaa cttcgcgacc gtcgccgagc cgctgcctta cagagcggcc
     3361 cgcaagcctt tgaggtagtc gagagtttcc tgcgctttgg cgcgctcgcc gtcgctcttc
     3421 gccagcgcga catcttcctc ggcgttcttg atccgcaggt cgagagtggc ggcgtccagt
     3481 tcggcgacag ggaccgcttc ctccgccagt accgtcaact tgccgggatt cacctcggcg
     3541 aacccgccca ggacatagaa acgctgatcg ccctccgata ccgtgatcac gccggggcgc
     3601 agggtggaaa tcaacggcat gtggcctgcc atcaccccca tatcgccttc gctgccggga
     3661 atggtgacca tctcggcttc cgtagacagc agcagccgtt cgggcgaaac cagatcgaag
     3721 gaaatcttgt cggtcatcga ctatgccgct tccgccgcca gtttctgggc cttcgccacg
     3781 gcgtcctcga tggtgccgac catgaagaac gcctgttccg gcagatggtc atattcgccg
     3841 ttcacgacgg ccttgaagct tctgatcgta tccttcaatt cgacgaactg gcccgggaag
     3901 ccggtgaaca cttcggcgac gtggaagggc tgcgacagga agcgctccag cttgcgggcg
     3961 cgcgccaccg cgatcttgtc gtcttccgag agttcgtcca tgcccaggat ggcgatgatg
     4021 tcctgcaggc tcttgtagcg ttgcagggtc tgctgcacct ggcgggcgac gtcgtaatgc
     4081 tcctggccga tgacgcgcgc gtcgagaatg cgcgaggtgg agtccagcgg gtccaccgcc
     4141 ggataaatac ccttttccga gatcgcgtgc gacagcaccg tggtggcgtc aagatgcgcg
     4201 aacgaggtcg ccggggcggg gtcggtcaaa tcgtcggccg gcacgtaaat ggcctgcacc
     4261 gaggtgatcg atcccttatt cgtggtcgtg atgcgttcct gcagctcgcc catgtcggtg
     4321 gccagggtcg gctgatagcc cacggccgaa ggaatgcggc cgagcagggc cgacacttcg
     4381 gaaccggcct gagtaaagcg gaagatgttg tccacaaaga acagcacgtc ctggccctgg
     4441 tcgcggaaat actcggcgac ggtcaggccg gtgaggccga cgcgggcgcg ggcgcccggc
     4501 ggctcgttca tctggccgaa caccagcgag catttcgagc cttcggtgga acccttcttc
     4561 ggatccttgt tgacgccgga ctcgatcatc tcgtgataaa gatcgttgcc ctcgcgcgta
     4621 cgctcgccga cgccggcgaa caccgaatag ccgccgtgct tcttggcgat attgttgatc
     4681 agttcctgga tgatgacggt cttgccgaca ccggcgccgc cgaacaagcc gatcttgccg
     4741 ccgcgggcgt aaggcgccag cagatcaatg accttgatgc cggtgaccag cacctgcgat
     4801 tccgtcgatt gatcgacgaa ggagggggcc tcggcatgga tcggccgcgt ttcagcgcct
     4861 tggatgggac cggcttcgtc caccggctcg ccgatgacgt tcatgatgcg ccccagcgtg
     4921 ccttcgccca ccggaaccgc gatggccagg ccggtgtcgg tgacctgctg gccgcggacc
     4981 agcccgtcgg tggaatccat cgcgatggtg cgtaccgtgg attcccccag atgctgggcc
     5041 acttcgagca ccagccggcg gccgtgattg tcggtctcta gcgcattctg gatttgcggc
     5101 agatgaccgt cgaactggac gtccaccacg gcgccggtga cctgcgtgac ccggcccaat
     5161 ttgctggtga ttacggcatt cattgcatta cttccttcaa atctgaactt actctttggc
     5221 gcattttctg cggcgaaccg ctgcacactt cgccggaaaa tgctttaaac gacggtcagc
     5281 gtgaccggca cgttgccgcg cgtcgccctg gaataggggc agatcgtgtg ggcgtcgtcg
     5341 agcagcgcct tcaccgcggc cttgtccgcg ccgggaacgg agagggtgat gtccaccttc
     5401 agggcgaaac tgatctcgtc cttgtccagc gtgacctgca cgccgaccac cgctttggcg
     5461 ggatcgatgc catgcttgcg gccgagcgca atcaccgcct gattgaagca ggccgcataa
     5521 ccgatggcga agagctgttc cggattgtgg ccgtcgcccg cgccgccgag ttcctttggg
     5581 aaggacatgg aaagcgccaa ggcgccgccg tccaggctgg cgcggccctc gcgcccgcct
     5641 ttgctcgttg ctttcgtcgt gtaagccatc gtctggctcc ttttcctaaa gcgcttccgc
     5701 gcccgaaatg atttcgatca gttccttggt gatctgcgcc tgtcgcgtcc ggttgtagag
     5761 cagggtcagc ttgtcgatca cttcgccggc gttgcgcgtg gcgccgtcca tcgcggtcat
     5821 cttggcgccg aattcgccgg cgacgttttc gagcagcgcg cggaaaatct gcacgttgac
     5881 gttgcgcggc agaagatcgg ccaggatttc gatttcgtcc ggctcgtact cgtaaaccgt
     5941 ggc
//
LOCUS       DASWPU010000523         5708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_17004,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000523 DASWPU010000000
VERSION     DASWPU010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17004"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1343)
                     /locus_tag="VGC27_11100"
     CDS             complement(129..1343)
                     /locus_tag="VGC27_11100"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY0283157.1"
                     /translation="MSAPALAISLDDVRAAAAAIEGAVERTPTRLSRTLSGIAGCDIW
                     LKFENLQFTASFKDRGALNKLLSLTAGERARGVIAMSAGNHAQGVAYHAGRLAIPATI
                     VMPLATPFNKVKHTKGFGARVVLEGATLSESFARAQKIAAAENLVFVHPYDDPKVMAG
                     QGTVALEMLADAPALDALVVPIGGGGLISGISVAAKAINPAIDVYGVQTRVYPSMYNA
                     VKGENLPCSGQTMAEGIAVKEPGALTTPIIRALVKDILLVGESDIEHAIASLLEIEKT
                     LVEGAAAAAYAAVIANRGLFAGRKVGLVLSGGNVDMRLLSNVIVRELSREGRILSLVI
                     EIVDMPGILARVSTLVGQAGGNILEVSHNRMMTDTSAKLADLGMTVEARDADHAEEIK
                     AKLEAAGFKLKA"
     gene            complement(1364..1438)
                     /locus_tag="VGC27_11105"
     tRNA            complement(1364..1438)
                     /locus_tag="VGC27_11105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1404..1406),aa:Met,seq:cat)
     gene            complement(1474..2604)
                     /gene="mnmA"
                     /locus_tag="VGC27_11110"
     CDS             complement(1474..2604)
                     /gene="mnmA"
                     /locus_tag="VGC27_11110"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY0283158.1"
                     /translation="MSAQSSKPRIVVAMSGGVDSSVTAALLKRDGFEVIGVTLQLYGG
                     APKRKGACCAGQDIYDAKRVAEKLGIAHYVLDYERRFRERVIADFVETYAKGETPIPC
                     VRCNERVKFADLLESAQALGAEALATGHYVRRVEGLHGVELHRAADESRDQSYFLFAT
                     TRAQLAYLRFPLGGMEKPAVRAIAAELGLGNAAKPDSQDICFVQDGRYGNLVEKLKPD
                     AARPGEIVDETGRVLGTHSGVIHFTVGQRKGLGLSGNAEQLFVLALDAARARVVVGPR
                     SSLRTRNIVLREVNWLADPAEARACAVKVRSMRAPLPARVAAGAGGMASVELAEAEEA
                     IAPGQACVFYKGDRVLGGGWIASAQNALAAGGGARPAALRAS"
     gene            complement(2647..3594)
                     /locus_tag="VGC27_11115"
     CDS             complement(2647..3594)
                     /locus_tag="VGC27_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY0283159.1"
                     /translation="MTVDRIATNTQAQYLLSQVMNASSALETTLAQIASGKTSSNYVG
                     IGDKTAALEAARSAAARADAYSSSTQLAVTQTDLQNTQLTALSNLAGELKQAIIDAAG
                     KSDGTSLMATADDIFQQASAILNSTDSNGNYIYGGGRGDGKPFTAATLSDLAGGDVSS
                     FFENGTQRKAVLVGDGQSVKIGLLASDIGTGLMIALKNLANADDPAGSLDGALSQTQT
                     ADLTNNVMPSATQAFTDLNAVTAVNGDTYNRLKDAVTNQKSLSTLYTGFVSDIEDVDM
                     AQAVTKLNQNQVALQAALAVTAQLGRLSLLNYLPSTTTG"
     gene            complement(3605..>5708)
                     /gene="flgK"
                     /locus_tag="VGC27_11120"
     CDS             complement(3605..>5708)
                     /gene="flgK"
                     /locus_tag="VGC27_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEY0283160.1"
                     /translation="LSALQANSAALRVVSNNISNLNTPGYARRVVNLETQSAGGQLAG
                     VNLSDVQRVVNQFLNQESLSAGASASNYDTQNTIFGQLNVLLGSPGSGTALTSKLSKV
                     FAALGQAALSPASSSSQVVALNAFQGLADSIASLSDSLSGLRRQVDSQVGASIGSANT
                     LIKQIYDFNRQIKLQSAAGNTDSALLDHRDAALQSLSQLMDVRTVQQPDGSMAVMTQD
                     GISLVGGSTYAQLSYTSGSSGSVFQPIMIQDVNAQNGQTIGTAQALDPHLSGGKLHGL
                     LAMRDGTLADLQNELGSFAQGVALSFNAQHNANSAYPPPTTLDGRNTGLLTGDALNFS
                     GKTTVAVTDSSGNLVKRIDLDFDGNRYSTDGGTTWTAFPSDDIGGMAAALNSALGPNG
                     TASFADGRLSISANGTNGIVVKDDATAPANRGGCGFSQFFGLNDLFQSAVPSILSTGL
                     TAGDASGLSAGDIVFALKDADGNAKQVKVTVPSGTTIGTVVDSLNSQMAGVASFTFNA
                     SDGSITAAVSSSYPGYQLLVDSDTTQRGSTGIGFTGLFGIGANQMAQQATSFSVTSAI
                     AASPSRLAFGRADLSSSGPIVGSDDSRGLLALQNLERAQQIFAKTGNLGQQAVTLGNY
                     AAGFYQDIATRGADIASSMIAQNDRLTEALARQSSTSGVNLDEELSNMVLYQQAYAAN
                     ARMLSVADQLLTTLLQIR"
BASE COUNT          958 a         1793 c         1912 g         1045 t
ORIGIN      
        1 atctttgttg cgctgtcaaa atccgggaac cgtcaactgg gtggcccgca cggagtttac
       61 actccggccg cgctttgcgc ggaccggggt gcgggccatg acaccgagtg ggtggaatgg
      121 gccccagctt acgccttcag cttgaacccg gccgcttcga gcttggcctt gatctcttcg
      181 gcgtggtcgg catcgcgcgc ttccaccgtc atgccgagat cggccagttt ggcggaggtg
      241 tcggtcatca tcctattgtg gctgacttcg agaatgttgc cgcccgcctg gccgaccagc
      301 gtcgagaccc gcgccagaat gcccggcatg tccacgatct cgatcaccag cgacaggatg
      361 cgcccctcgc gagacaattc gcggacgata acgttggaca acagccgcat atcgacgttg
      421 ccgccggaca gcaccagccc gaccttgcgg ccggcgaaca gcccgcggtt ggcgatcacc
      481 gccgcataag cggcggcggc ggcgccttcc accagcgtct tctcgatttc cagcagcgac
      541 gcgatggcgt gttcgatgtc gctttcgccg accagcagaa tgtccttgac cagcgcgcgg
      601 atgattggcg tggtcagcgc gccgggctct ttgaccgcga tgccttcggc catggtctgg
      661 cccgaacaag gaaggttctc gcccttcacc gcgttgtaca tggaaggata aacgcgcgtc
      721 tgcacgccgt agacgtcgat ggcgggattg atcgccttcg ccgcaacgga aattcccgaa
      781 attaatccac cgccgccgat gggcaccacc aatgcgtcga gcgcgggcgc gtcggccagc
      841 atctccagcg ccaccgtacc ctggccggcc atgaccttgg gatcatcgta gggatgcacg
      901 aagacgagat tctccgccgc cgcgatcttc tgcgcccgcg cgaaggactc cgacagcgtc
      961 gcgccttcca gaaccacgcg tgcgccgaag cctttggtgt gtttgacctt gttgaacggc
     1021 gttgccagcg gcatcacgat ggtggcggga atggcgagcc ggccggcgtg ataggcaacg
     1081 ccctgggcgt gattgccggc ggacatggcg atgacgccgc gcgcccgttc cccggcggtc
     1141 agcgacaaca gcttgttcag cgcgccgcgg tccttgaacg aggcggtgaa ctgcaaattc
     1201 tcgaatttga gccagatgtc gcagccggcg atcccggaca gggtgcgcga caggcgggtg
     1261 ggcgtgcgct cgaccgcgcc ctcgatggcg gcggcggcgg cgcgtacgtc gtcgagactg
     1321 atcgcaagcg ccggagcgga caagaaaagt tccttcgcaa gactggtagc agcggaggga
     1381 tttgaacccc cgaccaaggg attatgattc ccctgctcta ccactgagct acgctgccgc
     1441 ggcgggggat gtaggagcgc cgggcgggcc ctgtcaagaa gcgcgcaaag cagcggggcg
     1501 cgcacctccg ccggccgcca aggcgttttg ggccgaggcg atccagcccc cgcccaggac
     1561 ccgatcgccc ttgtagaaaa cgcaggcctg gccgggcgcg atcgcctctt ccgcctcggc
     1621 gagttcgacg ctcgccatgc cccccgcgcc ggccgccacc cgcgccggca acggcgcgcg
     1681 catggagcgg accttgaccg cgcaagcccg cgcctcggcc ggatcggcca gccagttgac
     1741 ctcgcgcaac acgatattcc gtgtacgaag cgatgaacgg ggtccgacga cgacacgcgc
     1801 gcgcgccgcg tccagcgcca gcacgaacag ttgctcggcg ttgcccgaaa gtccgagacc
     1861 cttgcgttgc ccgacggtga aatggatgac gccggaatgc gtgccgagca cccggcccgt
     1921 ctcgtcgacg atctcgcccg gccgcgccgc gtccggcttg agcttttcga ccaggttccc
     1981 gtagcgcccg tcctgcacga aacagatgtc ctggctgtcc ggcttggcgg cgttgccgag
     2041 gccaagttcg gcggcgatgg cgcgcaccgc gggcttctcc atgccgccga gcggaaagcg
     2101 cagataggca agttgcgcgc gcgtggtggc gaacaggaaa tagctctggt cgcggctttc
     2161 gtcggcggcg cggtgcagct ccacgccgtg gagcccctcg acgcggcgga cataatggcc
     2221 ggtcgccagc gcctcggcgc ccagcgcctg ggcgctctcc aagaggtcgg cgaacttcac
     2281 ccgctcgttg cagcgcacgc agggaatggg cgtctctccc ttggcgtagg tctcgacgaa
     2341 gtcggcgatg acgcgctcgc gaaagcgccg ttcgtaatcg agcacgtaat gggcgatgcc
     2401 gagcttctcg gcgacgcgct tggcgtcgta gatgtcctgc ccggcgcagc aggcgccttt
     2461 gcgtttcggc gcgccgccgt aaagctgcag cgtcacgccg atcacctcga aaccgtcgcg
     2521 cttgagcagc gccgcggtga cggaggaatc gacgccgccg gacatggcga ccacgatgcg
     2581 cggtttcgac gactgggcac tcatgggcgc ggtcttatcc gctggccccg gccctcgcaa
     2641 gtgcggttag ccggtcgtcg tactgggcag gtagttcagc agcgaaagcc ggcccaactg
     2701 ggcggttacc gccagagcgg cctggagcgc gacctggttc tgattgagct tggttaccgc
     2761 ttgggccatg tccacgtctt cgatgtccga gacgaagccc gtatagagcg tggaaagcga
     2821 tttctgattg gtgaccgcgt ctttgaggcg gttatacgtg tcgccgttca ccgccgtaac
     2881 ggcgttgaga tcggtgaagg cctgggtcgc cgagggcatg acgttgttgg tcaggtcggc
     2941 ggtctgggtt tggctcaacg cgccgtcaag gctgccggcc ggatcgtcgg cgttggcgag
     3001 gttcttcaac gcaatcatca gcccggttcc gatgtcggaa gcgagcaagc cgatcttcac
     3061 gctttggccg tcgcccacga gtactgcttt cctctgggtg ccgttctcga agaagctcga
     3121 tacgtcgccg ccggcgagat ccgacaacgt ggccgcggta aacggtttgc cgtcgccccg
     3181 gccgccgcca tagatgtagt tgccgttgga gtcggtcgaa ttgaggatcg ccgaggcctg
     3241 ttggaagatg tcgtccgccg tcgccatcag actggtgccg tcgctctttc cggcggcgtc
     3301 gatgatcgcc tgcttcaatt ctccggccaa attcgacagc gcggtcaatt gcgtattttg
     3361 caggtcggtc tgggtgaccg cgagttgggt gctggacgag taggcgtcgg cgcgggcggc
     3421 cgcggaccgc gccgcctcga gagcggccgt cttgtcgccg atgccgacat agttgcttga
     3481 ggttttgccg ctggcgatct gggccagcgt ggtctcaagc gcggaactcg cgttcatgac
     3541 ctgcgagagc agatactgtg cctgggtgtt cgtggcgatg cggtcgacgg tcatggcgca
     3601 ctccctaccg gatctgtagc agtgttgtga ggagctggtc ggcgacgctc aacatgcgtg
     3661 cgtttgcggc ataggcttgc tggtaaagca ccatgttgga cagttcctcg tccagattga
     3721 ctccggatgt cgacgattgg cgcgccaacg cttcggtcag gcggtcgttc tgcgcgatca
     3781 tgctcgaggc gatatcggcg ccgcgcgtgg cgatgtcctg ataaaaaccc gcggcgtaat
     3841 tgccgagcgt gacggcctgc tggccgagat tgccggtctt ggcaaatatc tgttgcgctc
     3901 tttccagatt ctgcaacgcc agcaggccgc gggaatcgtc gctgccgacg atgggcccgc
     3961 tcgacgaaag atcggcccgg ccgaaggcga gccggctcgg cgacgcggca atggccgagg
     4021 tgaccgaaaa gctggtggcc tgctgcgcca tctggttggc accgatgccg aacaatcccg
     4081 tgaagccgat gccggtggag ccgcgctgcg tcgtatcgct gtccaccagg agctgataac
     4141 ccggataact cgaggacacc gccgccgtga tcgacccgtc gctcgcgttg aacgtaaagc
     4201 tcgccacccc ggccatctgc gagttgagcg agtccaccac ggtgccgatg gtcgtgccgg
     4261 acgggaccgt caccttgact tgtttggcgt tgccgtcggc gtccttgagc gcgaaaacga
     4321 tatcgccggc cgacagcccg ctcgcgtcgc cggccgtcaa accggtcgaa aggatggaag
     4381 ggacggcgga ctggaacagg tcgttcaagc cgaagaattg cgagaagccg cagccgccgc
     4441 ggttcgccgg cgcggttgcg tcgtctttga cgacgatacc gttcgttccg ttggccgaaa
     4501 tcgagagccg gccgtccgcg aagctggcgg ttccattggg gccgagcgcg ctgttcaacg
     4561 ccgcggccat cccgccgatg tcgtcgctgg gaaaagccgt ccaagtcgtg ccgccgtcgg
     4621 tcgaatagcg attaccgtca aaatccaagt cgatgcgttt gacgaggttg ccgctggaat
     4681 ccgtcaccgc gaccgttgtc ttgccgctga aattgagcgc atcgcccgtc agaaggccag
     4741 tgttgcgccc atcgagcgtc gtcggcggcg gataggcgct gttggcgttg tgttgggcgt
     4801 tgaaggacaa cgcgacgccc tgcgcgaacg aaccgagttc gttctgcaga tcggccagcg
     4861 tgccatcgcg catggcgaga aggccgtgaa gtttgccgcc cgagaggtgc ggatcgaggg
     4921 cctgggccgt gccaatcgtc tggccgttct gcgcattgac gtcctgaatc atgatcggct
     4981 ggaagacgct gcccgagctg cccgacgtat aagagagctg cgcgtaggtg ctgccgccga
     5041 ccaggctgat gccgtcttgc gtcatcacgg ccatgcttcc gtcgggctgc tggacggtgc
     5101 gcacatccat caactgcgaa aggctttgca gtgccgcatc gcggtgatcc aggagcgccg
     5161 agtcggtgtt gcccgcggcg ctctgcaatt tgatctgccg gttgaaatcg taaatctgct
     5221 tgatgagcgt gttggccgaa ccgatcgagg cgcccacctg gctgtcgacc tgtcgccgca
     5281 ggccggacag ggaatccgat aaggacgcga tcgagtccgc cagcccttga aacgcgttca
     5341 gcgcgacaac ctggctcgac gacgatgccg gcgacaaagc cgcctggccc agcgccgcga
     5401 agactttgct caacttggaa gtcagcgccg tgccgcttcc cggtgagccg agcagaacgt
     5461 tgagctggcc gaagatggta ttctgggtgt cgtagttcga ggcggacgcg ccggcagaga
     5521 ggctttcctg attgagaaac tggttgacga cgcgctggac gtcggaaagg ttcacgccgg
     5581 cgagttggcc gcccgccgat tgtgtttcca gattgaccac gcggcgcgca tagcccggtg
     5641 tattgaggtt ggagatgttg ttcgagacga cgcgcagcgc cgccgaattg gcttgcagcg
     5701 cagacaaa
//
LOCUS       DASWPU010000524         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_108968,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000524 DASWPU010000000
VERSION     DASWPU010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108968"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1727
                     /locus_tag="VGC27_11125"
     CDS             <1..1727
                     /locus_tag="VGC27_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY0283161.1"
                     /translation="NGIAVGMATSIPPHNLGELCAATIALIENPNARDSTLLKYVKGP
                     DFPTGGIIIDDAESIAEAYQTGRGSFRVRARWHKENGTRGMYQLVVDQVPYQIQKAKL
                     IERIAELIEQKKVPLLDDVHDESAEDIRIVLTPKSRVVEPELLMEQLFRQTDLESRIP
                     LNMNVLDKGLVPRVMSLKEALLAFVAHRREVLERRSNYRLTRIATRLEVLEGYLAVFL
                     NLDKVIKIIRTEDEPKPVLMKAFKLTENQAEAILNMRLRSLRKLEEMEIRDEHGALTK
                     EQKELKKLLGSGKLMNERLVAEMKIIDAKFGLKTALGKRRTQIAEAKTVAKIQAIAEE
                     VVTIEAAVEREPITVVCSQKGWLRAFKGHQEPSEAIKYREGDRGRFWIHAETTDKLMM
                     FSTDGRFFTLECSKLPGGRGNGEAIRSFIDLPPEADLVEMFVHKEGRKLLLAATSGHG
                     FVTAEDEAVAMTKAGKRVMNVKAPGEAVVCAFVEGDSVAVIGENRKLVIFPLSEVPEM
                     ARGQGVRLQRYHDGALLDATAFTWKEGLRDQNNRTFAPSELKDWKGARAQAGRLPPRG
                     WAKSGKFA"
BASE COUNT          439 a          647 c          661 g          336 t
ORIGIN      
        1 cgaacggcat cgcggtcggc atggcgacct ccatcccgcc gcacaatctc ggcgagctgt
       61 gcgcggcgac catcgcgctg atcgaaaacc cgaatgcgcg cgactccacg ctgctgaaat
      121 atgtcaaggg gccggacttc cccaccggcg ggataattat cgacgacgcc gagagcatcg
      181 ccgaagccta ccagaccggg cgcggctcgt tccgcgtccg cgcccgctgg cacaaggaaa
      241 acggcacgcg cggcatgtat cagcttgtcg tcgatcaggt tccttaccag atccagaagg
      301 ccaagctgat cgagcgcatc gccgagctga tcgagcagaa gaaggtgccg ctgctggacg
      361 acgtgcacga cgagagcgcc gaagatatcc gcatcgtgct cacgcccaag agccgcgtgg
      421 tcgagccgga gctgctgatg gagcagttgt tccgccagac cgacctggaa tcgcgcatcc
      481 cgctcaacat gaacgtgctc gataagggcc tcgtgccccg cgtcatgagc ttgaaggaag
      541 cgctgctggc cttcgttgcg caccgccgcg aagtgctgga gcgccgttcc aactatcgct
      601 tgaccaggat cgcgacccgg ctggaagtgc tggaaggcta tctcgccgtc ttcctcaatc
      661 tcgacaaggt catcaagatc atccgcacgg aagacgagcc caagcccgtc ctcatgaaag
      721 ccttcaagct caccgaaaac caggccgaag cgattctcaa catgcgcctg cggtccttgc
      781 gcaagctgga ggaaatggaa atccgcgacg aacacggcgc cctgaccaag gagcagaagg
      841 aactgaagaa gctgctcggc tccggcaagc tgatgaacga gcggctggtc gccgagatga
      901 aaatcatcga cgccaagttt gggctgaaga ccgcgctcgg caagcgccgc actcagatcg
      961 cggaagccaa gaccgtcgcc aagatccagg ccatcgcgga agaggtcgtc accatcgagg
     1021 ccgcggtgga gcgcgagccg atcaccgtgg tgtgctcgca aaagggctgg ctgcgcgcct
     1081 tcaagggcca tcaggaaccc agcgaggcga tcaaatatcg cgagggcgac cgcggccgct
     1141 tctggattca cgccgaaacc accgacaagc tgatgatgtt ttcgaccgac gggcgtttct
     1201 tcacgctgga atgctccaag ctgccgggcg ggcgcggcaa cggcgaggcg atccgcagct
     1261 tcatcgacct gccgccggaa gccgatctgg tggagatgtt cgtgcataag gaaggccgca
     1321 agctgctgct ggcggcgacg tccggccacg gcttcgtcac cgccgaagac gaagcggtgg
     1381 cgatgaccaa ggccggcaag cgggtgatga acgtgaaagc acccggcgaa gccgtggtct
     1441 gcgcctttgt cgaaggcgac agcgtcgcgg tgatcggcga gaaccgcaag ctggtgatct
     1501 tcccgctctc cgaggtgccc gagatggcgc gcgggcaggg cgttcgcctg caacgctacc
     1561 atgacggcgc gctgctcgac gccacggcct tcacctggaa ggaaggcttg cgcgaccaaa
     1621 acaaccgcac cttcgcgccg tccgaattga aggactggaa aggcgcccgc gcccaggccg
     1681 gccggcttcc cccgcgcggc tgggcgaagt cgggcaagtt tgcctgagtt ccagtctcgc
     1741 tcggcgcgcc gaagccgcag gcgaaggcgg gctgggcgaa gagcgggaaa tttggctaga
     1801 gcaaaatgca ttcagacgga atcgcccctc acccttcggc aagtcagagt gagggcattt
     1861 tctaatccct catgctgagc ttgtcgaagc atgaagacaa cgtgatctcg tctgaacgta
     1921 cgaagctcta gccgccgcta gtgcagagtg cattcagatg gaaccgtttg tcattcccgg
     1981 ccgaccccgg acttgatccg ggggagggga agggaatcca tgagttgcgc aggtttctgg
     2041 atggttcata cgctgtcgct atgaactccc tcgcgctttg cgc
//
LOCUS       DASWPU010000525         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_53337,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000525 DASWPU010000000
VERSION     DASWPU010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53337"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VGC27_11130"
     CDS             <1..293
                     /locus_tag="VGC27_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0283162.1"
                     /translation="EELSDHPFIGDIRGLGLLAGIEFVADRATKARFPGKLNVAGRIK
                     AELLKRGLCTRVLSDVICLAPPLICTEQQIDQIVGIVVSTLSQPFDQFTSPL"
     gene            311..850
                     /locus_tag="VGC27_11135"
     CDS             311..850
                     /locus_tag="VGC27_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283163.1"
                     /translation="MPRIPFVTLETASPELKAEYEKLAGHHEVTNMKATLLHSPVALH
                     AVLEWYKLFDRVTPFLGERLATLYCDAISRQNRCELCASFMKREIVKWGEDPDNLKLD
                     DRDQTVIAFGRQLAADANKVSDALYAKLESYFTPSQIVDITVFGTLMIVNNLFNSALR
                     IPLDDSLDGYHVDPETYFA"
     gene            862..>1948
                     /locus_tag="VGC27_11140"
     CDS             862..>1948
                     /locus_tag="VGC27_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0283164.1"
                     /translation="MGERKLGFDTQQLHAGHVLDAAHRARAVPIYQSASFVFKDFASA
                     KAINAIEEFGYEYSRTGNPTNDVLEVRIAALEGGTAALSLASGAAAVTYSVLNVAHEG
                     DEVAVGRTLYGGTYSLFAHTLPKHGIKAVFVDSDEPENFRAAVNERTRAIFVETIGNP
                     AVNIVDVEAVGRIAAANGIPLIVDNTFATPYLYRPFEHGANVVVHSATKYIGGHGNSI
                     GGLIVDGGNFNWANGKFPGFTAPDASYNGIVHWERWGDYPGIGNFAFVMKARLQYLRD
                     MGACLSPFNAFLLLLGLETLSFRVERQVTTTQKIAEHLAAHPDVAWVNYPGLRDNRYH
                     DLARRMIPKGPGAILSFGIKGGIARTVK"
BASE COUNT          389 a          638 c          550 g          371 t
ORIGIN      
        1 gcgaagaact ctccgatcat ccgttcatcg gcgacatccg cgggctcggc cttctggccg
       61 gcatcgaatt cgtggccgac agggccacca aggcgcgctt ccccggcaaa ttgaacgtcg
      121 ccggccgcat caaggccgag cttctgaagc ggggcctgtg cacgcgcgtc ttaagcgacg
      181 tcatctgcct ggcgccgccg ctgatctgta ccgagcaaca gatcgaccag atcgtgggca
      241 tcgtcgtcag tacgctgtcc cagccgttcg accaattcac cagccccctc taagacctgg
      301 agaagagccc atgccccgca ttcccttcgt aaccctcgaa accgcctcgc cggaattgaa
      361 ggcggaatac gagaagcttg ccggacacca tgaagtgacc aacatgaagg cgacgctcct
      421 gcactcgccg gtggcgcttc atgccgtcct cgaatggtac aagctgttcg accgcgtgac
      481 gccgtttctc ggcgagcgcc tggcgacgct gtactgcgac gcgatttcgc gccagaaccg
      541 ttgcgaactt tgcgcctcct tcatgaagcg cgaaatcgtc aaatggggcg aagacccgga
      601 caatctgaaa ctggacgacc gcgatcaaac cgtcatcgcc ttcggccgcc aactcgccgc
      661 ggacgccaac aaagtgtcgg acgcgcttta tgccaagctg gaatcctact ttactccttc
      721 ccagatcgtg gatatcacgg tgtttggcac gctgatgatc gtcaacaatc tcttcaacag
      781 cgcgcttcgt attccgctcg acgactcgct cgacggctat cacgtcgatc ccgaaaccta
      841 tttcgcgtga cggctgcttc gatgggtgaa cgcaaactcg gcttcgacac gcaacagctc
      901 catgccggac acgtgctcga cgccgcccac agggcgcggg ccgtgcccat ctatcaatcc
      961 gcgtctttcg tcttcaagga tttcgcctcc gccaaagcca tcaacgccat cgaggagttc
     1021 ggctacgaat acagccgcac cggaaacccc acgaacgacg ttctcgaagt ccgcatcgcc
     1081 gccctggagg gcggcacggc cgctttgtcg ctggcatccg gggccgccgc cgtaacctat
     1141 tccgttctca atgttgcgca tgagggcgac gaagtcgcgg tgggccggac gctctatggc
     1201 ggaacctaca gcctgttcgc ccataccttg cccaaacacg gaatcaaggc ggtattcgtc
     1261 gactccgatg aaccggagaa ctttcgggcc gctgtgaacg agcgcacccg cgcgatcttt
     1321 gtcgagacga tcggaaatcc cgcagtgaac atcgtggatg tcgaggcggt ggggcggatc
     1381 gccgcggcaa acggcattcc attgatcgtc gacaacacct ttgccacgcc ctacctctat
     1441 cggcctttcg agcacggcgc caacgtcgtc gtgcattcgg cgacgaaata catcggcggc
     1501 cacggcaaca gcatcggagg gttgatcgtc gacggcggca atttcaactg ggcgaacggg
     1561 aaattccccg gctttaccgc gccggacgcc tcctataacg ggatagtcca ttgggaacgt
     1621 tggggggatt accccggcat cggcaatttc gccttcgtga tgaaagcgcg tctgcaatac
     1681 ttgcgcgaca tgggggcctg cctcagcccg ttcaatgcct tcctgctgct gcttgggctt
     1741 gaaacgcttt ccttccgcgt cgaacgccag gtaaccacga cgcagaagat tgcggaacat
     1801 ctcgcggcgc atcccgacgt cgcctgggtc aattacccgg ggctccgcga caaccgctat
     1861 catgacctcg ccaggcgcat gatcccgaag gggccgggcg ccattctgtc gttcggcatc
     1921 aagggcggga ttgcgcgaac ggtgaagt
//
LOCUS       DASWPU010000526         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_23326,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000526 DASWPU010000000
VERSION     DASWPU010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23326"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2011)
                     /locus_tag="VGC27_11145"
     CDS             complement(<1..2011)
                     /locus_tag="VGC27_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY0283165.1"
                     /translation="MISQRSRRHEAQAGRITAVLGPTNTGKTHFAIERMLAHKSGMIG
                     LPLRLLAREVYDKISRLKGTNLTALVTGEEKIVPPHAKYFVCTVEAMPIDLGAAFLAI
                     DEIQLCADPERGHIFTDRLLHARGEEETVFLGADTMRGAIQHFVPRTYFTSRPRFSDL
                     AYTGSKKLTRLPRRSVVVGFSTEDVYGIADLIRSQRGGAAVVLGALSPRTRNAQVALY
                     QSGDVDFLVATDAIGMGLNMDVDHVAFSAMEKFDGFVTRPLKPEEIGQIAGRAGRHMN
                     DGTFGITGDAEALDEETVARVEGHRYEPVRVLQWRNSALSFRALDALIASLDEPPPAR
                     GLVRARPSSDVIALRTLSEQDEVRDRTRAPAQVRLLWDACQLPDFHKLSSEEHARLVR
                     TIFLHLTSGDGTLPEDWLAKQIARLDVTEGDVATLSGRLAQVRTWTYAANRPGWVKDG
                     AHWQEVTRAVEDRLSDALHEKLTQRFIDRRTSVLMRQLALDDIALVLDDSGGVAIGGE
                     IVGKLDGFRFSQAESAAGIHERALRAAAMKGLESEFFARARRLAFAADAEITLSEHGR
                     LWWDGAIVARLTAGPSPLAPNVALLADEILKGSPRESIQRRLDEWLSARIAAKLAPLL
                     ALRAGVEAKAGTADAMPAAARGIAHQLCENFASLQTAQATLPLDRR"
BASE COUNT          387 a          796 c          766 g          423 t
ORIGIN      
        1 cccgccgatc caacggcaag gtcgcctggg cggtttgcag cgaagcgaaa ttctcgcaaa
       61 gctgatgggc gatgccgcgc gccgccgccg gcatggcgtc ggcggtcccg gccttcgcct
      121 ccacgcccgc gcgcagggca agcaaaggag cgagcttcgc ggcgatgcgc gccgacagcc
      181 attcgtccag gcggcgctga atgctttcgc gcggactccc cttcagaatt tcgtcggcca
      241 gcagggccac gttcggcgcc agcggcgagg ggcccgccgt cagccttgcc acgatggcgc
      301 cgtcccacca tagccgccca tgttcgctga gggtgatctc ggcgtcggcc gcgaaggcca
      361 gcctccgggc gcgggcgaaa aattcgctct ccaatccctt catcgccgcg gcgcgcagcg
      421 cgcgttcgtg gatgccggcc gcgctttcgg cttgcgagaa gcggaatccg tccagcttgc
      481 cgacgatctc gcccccgatc gccacgccgc cggaatcgtc gagcaccaga gcgatgtcgt
      541 cgagcgccag ttggcgcatc agcaccgagg tgcgccggtc gatgaatcgt tgcgtcagct
      601 tttcgtgcag cgcgtccgac agccggtcct ccaccgcgcg ggtgacctct tgccagtgcg
      661 cgccgtcttt cacccagccc ggccggttcg cggcataggt ccaggtgcgg acctgcgcca
      721 gccgtcccga tagcgtcgcc acgtcacctt cggtgacatc gagccgggcg atctgcttcg
      781 ccagccaatc ctccggcagg gttccgtcgc cgctggttag atggaggaag atcgtgcgca
      841 ccagccgcgc atgttcctcg ctgctgagct tgtggaagtc cggcaactgg caggcgtccc
      901 acagaaggcg cacctgcgcc ggcgcgcgcg tcctgtcgcg cacctcgtcc tgctcgctca
      961 gggtccgcag cgcgatcacg tcgcttgagg gacgcgcccg caccaggccg cgcgccggcg
     1021 gcggctcgtc gaggctggcg atcagcgcat cgagcgcgcg gaacgacagc gccgagttgc
     1081 gccattgcag cacgcgcacc ggctcgtagc ggtggccttc gacgcgcgcc acggtttcct
     1141 cgtccagcgc ctcggcgtcg ccggtgatgc cgaaggtgcc gtcgttcatg tgccgtccgg
     1201 cgcgccccgc gatctgtccg atttcttcgg gtttcagcgg gcgggtcacg aagccgtcga
     1261 acttctccat cgccgagaag gcgacatggt ccacgtccat gttgaggccc atgccgatgg
     1321 catcggtcgc caccaggaag tccacgtcgc cggattggta gagcgccacc tgggcgtttc
     1381 gcgtccgcgg agacagggcg cctaagacca ccgccgcccc gccgcgctgg ctgcggatca
     1441 ggtcggcgat accgtaaacg tcttcggtgg agaaaccgac cacgaccgaa cgccgcggca
     1501 gccgcgtcag cttcttcgag ccggtgtatg ccaagtccga gaagcgcggc cgcgaggtga
     1561 aataggtacg cggcacgaaa tgctggatcg ctccgcgcat cgtgtcggcg ccgagaaaca
     1621 cggtctcctc ttcgccgcgc gcgtgcagaa gcctgtcggt gaagatgtgg ccgcgttcgg
     1681 gatcggcgca aagctggatt tcgtcgatcg ccaggaacgc cgcgccgaga tcgatcggca
     1741 tcgcttccac cgtgcagacg aagtatttgg cgtggggcgg aacgatcttc tcttcgccgg
     1801 tgacgagggc ggtgaggttc gtgcctttca gccgcgagat cttgtcatag acctcgcgcg
     1861 ccagcaggcg cagcggcagg ccgatcatgc cggacttgtg ggccagcatc cgctcgatgg
     1921 cgaaatgggt cttgccggta ttggtggggc cgaggaccgc ggtaatccgg cccgcctgcg
     1981 cctcatgcct gcgcgaacgc tgtgagatca ttatgagcgg aagatggggt ttttcgattt
     2041 gcggtacaag caaggccctg caggcaggcc gcgccgaggc gttcttcaac gcctggaata
     2101 taaagcgaac gaacgcagcc cgaatcacta accaactcaa tatcggcctt tgttctcacg
     2161 ctattaggtg tttgtcaatc aattcctatc gcaacatctt gtggattggt cgagccatgg
     2221 agtctcgcag acttatccac gtaacactcc gtatgcggaa caatgtgacg atttcccgac
     2281 atttccaaga cggcggtttg accgtacttt cgcgggtgcg atactccccc cgcgctatcg
     2341 caccgcatgt ccgaaccggc tcgcgtgcca cg
//
LOCUS       DASWPU010000527         1876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_23334,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000527 DASWPU010000000
VERSION     DASWPU010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23334"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VGC27_11150"
     CDS             complement(<1..554)
                     /locus_tag="VGC27_11150"
                     /inference="COORDINATES: protein motif:HMM:NF005458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY0283166.1"
                     /translation="MKSALAIRHVHFEDLGAFGPVLENAGYEAGYVQVGVDSIAQAGA
                     EVADVLIVLGAPIGAYEEDKYPFLLETMRVLEARLAAGRPTLGICLGAQLMARALGAR
                     VYPGPAKEIGWAPVSLTEEGRQSALRHLEGKPMLHWRSDTFDLPEGAIRLASTALTPN
                     QAFSYGPHALGIQLHPEAVGEKFEL"
     gene            1041..1295
                     /locus_tag="VGC27_11155"
     CDS             1041..1295
                     /locus_tag="VGC27_11155"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0283167.1"
                     /translation="MEAYGIIAWLIIGAVAGWLAGKLVKGGGFGLLGNIVVGIVGALI
                     GGWLAGALGISIGSGLIASILTATLGAVILLVILRMIKRT"
     gene            1459..>1876
                     /locus_tag="VGC27_11160"
     CDS             1459..>1876
                     /locus_tag="VGC27_11160"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY0283168.1"
                     /translation="MALKRATASVSHKKPDRPRNNARGTKPELSYKENQAEGGETHQT
                     AANGVETLTTQQGIPVADDQNTLRYGARGPALLEDAHFREKIFHFDHERIPERIVHAR
                     GYGAHGYFELTESLAKVTRADVFQRVGEKTPAFVRFS"
BASE COUNT          396 a          547 c          543 g          390 t
ORIGIN      
        1 agctcgaact tttctccaac ggcctcggga tggagttgaa tccccaaggc atgagggccg
       61 taggaaaagg cttggttcgg cgtcagcgcc gtcgatgcca gcctgattgc accctccggc
      121 aagtcgaagg tgtcgctacg ccaatgcagc atgggcttgc cctcaagatg ccgcaacgcg
      181 ctctgcctgc cttcttccgt cagcgaaacc ggggcccatc cgatctcctt cgcggggccg
      241 ggatagacgc gcgcgccgag cgcccgcgcc atcaattgcg cgcccaggca aatgccgagc
      301 gtcgggcgcc ccgcggcgag ccgtgcttcc aacacgcgca tcgtttccag caggaaggga
      361 tatttgtctt cctcataagc cccaatcggc gcgcccagca cgatcagaac atccgccacc
      421 tccgcgccgg cttgcgcaat cgaatcgacg cccacctgaa cataaccggc ttcgtacccc
      481 gcattttcaa ggaccggccc gaacgcgccg aggtcctcga aatgcacatg gcgaatggcg
      541 agcgcggatt tcaagaaagg aatctccctg gcggctgata tcacgcagct taaagaagca
      601 atcgcgccgc cggaagtctc tccatccgac gcagcagcgc gccttgatga tgccgctctg
      661 cgagcgcgtc gaacgcaggt ttcggaacaa gccgcaagaa ttggccgaaa atcgtgctat
      721 gatagctcaa ggcttggttc tgcgttgtga atcaatggtg tgtcgcaact ttattgtatc
      781 tctcaacggg caaaccaagc cgtctttact tcttttttac tttactctct gggggacaga
      841 tgtggaggag atcagaatgt ctctgcgata gtcttcgcag ctcccctcca atagtcgctc
      901 cagcaaagac tagattgatt attttattga ttctaacaat tgaaggttgt aaatctatga
      961 ggcatcgcgt gagatttgac gtcgcgttcg gatgacgctt cgcgttccgc tttttcaaat
     1021 caaattttgg gaggaatcac gtggaagcat acggaataat cgcatggctt atcatcggcg
     1081 ccgttgccgg ctggcttgca ggcaagctgg tcaaaggtgg cggatttggg ttgctcggca
     1141 acatcgtggt gggcatcgtc ggcgccctca tcggtggctg gcttgccggg gccctgggca
     1201 ttagcatcgg cagcggcctg atcgcctcga tcctcacggc gacgttgggt gccgtcatcc
     1261 tcctcgtcat tctcaggatg ataaagcgaa cctgacagtt cgccattcgg tttcgctgcc
     1321 tggggaacgc gcgggcagag tgaaggacct tccagatgcg caggagcgtt ttcgggaacg
     1381 ggcacgagcg ggtttccact accggcttcg gctttgcaga cggctgatcg ataaaattcc
     1441 gcaagggagg attaccccat ggcgttaaag agagctacgg cttcggtatc ccacaagaaa
     1501 cctgaccggc cacgcaacaa tgcgcggggc accaaacctg aacttagcta caaagaaaat
     1561 caggcggaag gcggcgagac gcaccagacc gctgcgaacg gcgtcgaaac cttgaccacg
     1621 cagcagggca tcccggtcgc cgacgaccag aacacgctgc gctatggtgc acggggcccg
     1681 gctctcctgg aggacgcaca ttttcgggag aagattttcc acttcgatca cgagcgcatt
     1741 ccggagcgca tcgttcatgc gcgcggttat ggggcccatg ggtatttcga attgaccgag
     1801 tcgcttgcga aagtgacgcg tgccgatgtc tttcagcgcg taggcgagaa aacgcccgcc
     1861 ttcgtgcgtt tctcga
//
LOCUS       DASWPU010000528         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_59665,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000528 DASWPU010000000
VERSION     DASWPU010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59665"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1088
                     /locus_tag="VGC27_11165"
     CDS             138..1088
                     /locus_tag="VGC27_11165"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY0283169.1"
                     /translation="MQQRVLITGGAGFLGSFLCEALLKSGHEVLCVDNFYTSSRRNIE
                     HLIDHKRFELMRHDVTFPLYVEVDKIYNLACPASPIHYQRDPVQTTKTSVHGAINMLG
                     LAKRVKATILQASTSEVYGDPSVHPQTEEYWGNVNTIGPRACYDEGKRCAETLFFDYH
                     RQHGLRIKVARIFNTYGPRMHPNDGRVVSNFIMQALRNEPITLYGDGRQTRSFCYVDD
                     LVAGLIALMETRDEVTGPVNLGNPHEFTMLELAEKTVTIIGSRSKLEFKPLPTDDPKQ
                     RKPDISRAKAVLGWAPKIELDEGLKKTVAYFEGLLSEGLA"
     gene            1126..>1987
                     /gene="galE"
                     /locus_tag="VGC27_11170"
     CDS             1126..>1987
                     /gene="galE"
                     /locus_tag="VGC27_11170"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY0283170.1"
                     /translation="MTNILVTGGAGYIGSHACKALAKAGYTPVAYDNLVYGHEWAVRW
                     GPLEKGDILDPVRLAEVMTKYRPAAVMHFAAFAYVGESVTDPAKYYRNNVMGTLTLLD
                     AMRAHEVGQIVFSSSCATYGVPKRIPISEDAPQQPINPYGVSKLMVERILADYAAAYG
                     LKSVALRYFNAAGADTDGDIGEDHDPETHLIPLVLDAAAGSRPDITIFGSDYATPDGT
                     CIRDYVHVSDIADAHVLALKKLDGGGLRAAYNLGTGAGISVAQAIAAATKVTGRPVRV
                     THGARRAGDPD"
BASE COUNT          409 a          605 c          604 g          369 t
ORIGIN      
        1 gttggcgaaa ggtcgagttc acaaaaccgg atgacgcccg agtcacccgg acgacggtag
       61 tttgctttcg cctcatgtga gcgtcacttg ggttcgaggc ggtaatccgg ccgtcgaagg
      121 gtgaggaaac gatgcatatg caacagcgcg ttctgatcac gggcggggcc ggctttctcg
      181 gctccttcct ctgcgaagca ttgctcaaga gcgggcacga agtgctctgc gtcgataatt
      241 tctacacgag ctcgcggcgc aacatagagc acctgatcga tcacaagcgg ttcgaactga
      301 tgcgccacga cgtgacgttt ccgctctacg tggaagtcga caagatatac aatctggcct
      361 gtccggcttc tcccatccac tatcagcgcg atcccgttca aacgacaaag acgtcggtcc
      421 acggcgccat caacatgctg ggcctggcca agcgcgtgaa ggcgacgatt ttgcaggcgt
      481 cgaccagcga agtgtatggc gatcccagcg ttcatccgca aacggaggaa tattggggca
      541 acgtcaacac catcggcccg cgcgcctgtt acgacgaggg caagcgttgc gccgagacgc
      601 tgtttttcga ttatcaccgc cagcacggtt tgcgcatcaa ggtcgcgcgc atcttcaaca
      661 cttacggccc gcgcatgcat cccaatgacg gccgggtggt ctccaacttc atcatgcagg
      721 ccctgcgcaa cgagccgatc accttgtacg gcgacggccg gcaaacccgt tcattctgct
      781 atgtcgacga tctcgttgcc ggattgatcg cactgatgga aacgcgcgac gaagttaccg
      841 gccccgtcaa tctcggcaat ccgcacgaat tcaccatgct cgaacttgcc gaaaaaaccg
      901 tgacgatcat cggttcgcgc tcgaagctgg aattcaaacc gctgccgacg gacgatccga
      961 aacagcgcaa gccggatatt tcgcgcgcca aggcggttct ggggtgggcg ccgaagatag
     1021 aactcgacga aggcttgaaa aaaacggtgg catatttcga gggattgctt tcggaaggtc
     1081 tggcatagtg aaaagccttc cgatccgtgc gggccgcagc gggcgatgac gaatattctc
     1141 gtcaccggcg gcgcgggata tatcgggtcg cacgcgtgca aggccctcgc caaggcggga
     1201 tacacgccgg tcgcctacga caatctcgtt tatggccatg aatgggcggt ccggtggggc
     1261 ccgctcgaga agggcgacat cctcgatccg gttcgcctcg ccgaggtcat gacgaaatac
     1321 cggccggccg ccgtcatgca tttcgcggcg ttcgcctatg tcggcgagtc ggtcactgat
     1381 ccggcgaaat actaccgtaa caacgtcatg gggaccctta ccttgctcga tgcgatgcgc
     1441 gcgcacgagg ttgggcaaat cgtgttttca agctcgtgcg cgacctatgg cgttcccaaa
     1501 aggatcccga tctcggaaga cgctccgcag caaccgatca atccctacgg cgtctccaag
     1561 ctgatggtcg agcgcattct tgccgattat gcggccgcat acggactgaa gagcgttgca
     1621 ttgcgctatt tcaatgcggc cggcgccgac accgacggcg acatcggcga ggaccacgac
     1681 ccggaaaccc atctcatacc gctggtgttg gatgcggcgg cgggctcccg tccggacatc
     1741 acgatcttcg gcagcgacta cgcgacgccg gacggcacct gcattcgcga ctacgtccat
     1801 gtcagcgaca tcgcggacgc gcacgtgctc gcgctcaaga aattggacgg cggcggattg
     1861 cgcgccgcgt ataatctggg caccggcgcg ggcatatcgg tcgcgcaggc gatcgcggcg
     1921 gcgaccaagg tcacggggcg gcccgttcgg gtgacgcacg gcgcgcggcg cgcgggcgat
     1981 cccgata
//
LOCUS       DASWPU010000529         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_53362,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000529 DASWPU010000000
VERSION     DASWPU010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53362"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VGC27_11175"
     CDS             complement(<1..886)
                     /locus_tag="VGC27_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0283171.1"
                     /translation="MIYLDHNATSPLRAEAKEAGERALGIGGNASSVHGRGRAARAVI
                     EHARVQVAALAGVRIEDVIFTSGGSEANALALWGAIHGAAADGARITRLFVSAIEHES
                     VLANAAALAERVAGLRLEFIAVTGDGGADLEALRAMLREGKGRALIALMAANNETGVL
                     QPIAEVAELAQEAGALLFVDAVQAAGKIDLPKADYLSFSAHKIGGPQGVGALILRAGA
                     PFAAQILGGGQEQGRRAGTENLSGIAGFGAAAEITARELSAAKEIAVLRDRFEDGLKR
                     VAPDVTIFGVHAPRLANTS"
     gene            complement(902..1375)
                     /locus_tag="VGC27_11180"
     CDS             complement(902..1375)
                     /locus_tag="VGC27_11180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0283172.1"
                     /translation="MRLSTKGRYAVMAMTDLAMNEAGARPVSLADIAERQEISLSYLE
                     QLFAKLRRNGLVSSVRGPGGGYRLSRPADEVRVADIIMAVDEPINATRCRPGSAKGCA
                     TQGARCITHDLWEELGRQIHVFLSSISLADVVEKRVLGRAQLDLQPQSGDNRAAA"
BASE COUNT          291 a          536 c          465 g          298 t
ORIGIN      
        1 tcgaggtgtt ggcaagacgc ggagcatgaa cgccgaaaat ggttacatca ggcgccaccc
       61 gcttcaagcc atcttcgaag cgatcgcgca gcacggcaat ttccttcgcc gcggacagtt
      121 cacgcgcggt gatttcagcg gcggcgccga agcccgcgat gccggagaga ttctcggtgc
      181 cggcacggcg gccttgctcc tgtccaccgc ccaggatttg cgccgcgaac ggcgcgccgg
      241 cacgcaggat caaggcaccc acgccttgcg gtccgccgat cttgtgggcc gagaacgaaa
      301 gataatccgc cttcggcaga tcgatctttc ccgcggcctg tacggcgtct acgaaaagca
      361 gcgcgcctgc ttcttgcgcc agctccgcaa cctcggcaat cggctgcaac acgccggtct
      421 cgttgttggc cgccatcagc gcgatcaggg cgcgcccttt gccctcgcgc agcatcgccc
      481 gcaacgcttc gagatcggcg ccgccgtcgc ccgtcaccgc aatgaactcg agccgcagac
      541 ccgcaacccg ttcggcaagc gccgccgcat tggccagaac ggattcatgt tctatggccg
      601 agacgaacag ccgggtaatg cgcgccccgt cggcggccgc gccatggatg gcgccccaga
      661 gcgccagagc gttggcttcg ctgccgccgc tggtgaaaat tacatcctcg atccggaccc
      721 ctgccagtgc cgcaacctgt acgcgcgcgt gctcgatgac ggcgcgcgcg gcgcgtccgc
      781 gcccatgcac cgacgaagca ttgccgccga tgccgagcgc gcgctccccc gcttctttcg
      841 cctcggcgcg caaaggtgag gtggcattgt gatctagata aatcattact ctcacatacc
      901 ctcaagccgc cgcacggttg tctccggatt ggggctgtag atcaagttgg gcccggccca
      961 atacgcgctt ctccaccaca tcggcaagcg atatggacga cagaaatacg tgaatctgcc
     1021 ggccgagttc ctcccagaga tcgtgtgtaa tgcagcgcgc gccctgggtg gcgcaaccct
     1081 tggcactgcc cggcctgcaa cgcgtggcat tgatgggttc gtccaccgcc atgatgatat
     1141 cggcaacacg gacttcgtcc gctggccggc tcagccggta gccgccgccc gggccgcgca
     1201 cgctcgacac caggccgttg cggcgcagct ttgcgaacag ttgctccaga taggaaagcg
     1261 agatttcctg ccgttcggcg atatcggcca acgaaaccgg gcgcgcgccc gcctcgttca
     1321 tggccagatc tgtcatcgcc atcaccgcat agcggccttt ggtactgagt ctcataacct
     1381 cttgctccaa accgcctcgc gcgttttcct tgctttcagg ggggccgctt ggtgtaagaa
     1441 aagcggtcta ccgcgtggcg ttctttcact gcggatatag gattcctgag taccctggtc
     1501 aagaagtcct ccagaccaat cacgaagtat gtactatgcc actgtttttg aacggtttta
     1561 gattgtggca cggcaagctg gaaatccaca
//
LOCUS       DASWPU010000530         3984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_17067,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000530 DASWPU010000000
VERSION     DASWPU010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17067"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1000
                     /locus_tag="VGC27_11185"
     CDS             242..1000
                     /locus_tag="VGC27_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283173.1"
                     /translation="MIRNCLLAAAATAMLSAGAGAAPVPATVKAALNDAARPADDVKR
                     DADRKPAAMLAFAGIKPGARVMDLIPGGGYFTRIFAKAVGPSGYVYAFQPTELDHFFK
                     DKEPPIVVVAAAYPNVSVIHAPVNTLAAPESLDVVWTSQNYHDLKNDFFKPADSALVN
                     KAVYDALKPGGLYIVLDHSAPEGSGVRDTDTLHRIDEAVVKKEVEAAGFKLAGESNVL
                     RNPKDQRTVKVFDPSIRGHTDQFILKFRKPAGHK"
     gene            997..1251
                     /locus_tag="VGC27_11190"
     CDS             997..1251
                     /locus_tag="VGC27_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283174.1"
                     /translation="MMREGKHRGVLMIGALPVGTVVIAALALGALSIGALAIARMAIG
                     RVAVGKLRLKRAEIGELTVHTLRVLDNKPHEPAHPSTGSG"
     gene            complement(1392..>3984)
                     /locus_tag="VGC27_11195"
     CDS             complement(1392..>3984)
                     /locus_tag="VGC27_11195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY0283175.1"
                     /translation="FVFALLLALVPQAVQAAPDRVVLPADVVPAHYDLSIVPDAAAMT
                     FTGAVKIDIEVREQTSRIELNAADLTFSRVALSGVAGQPQVAFDKTKETATLTFAAPI
                     APGRYVLAIDYAGKINQHAAGLFYLDYDTEAGTKRALFTQFENSDARRFVPCWDEPAR
                     KASFVLAATVPADAMAVSNMPIAAREMLADGRAHVRFAETPVMSSYLLFFGLGDFERV
                     VRQVDGVEIGVIVKRGDTAQAQYALDAAVQILPYYEDYFGVKYPLPKLDLIAGPGQSQ
                     FFGAMENWGAMFFFERTLLIDPKISTESDKRGVYVTVAHEMAHQWFGDLVTMEWWDGI
                     WLNEGFASWMELKATNRFNPQWNTWLRSLGSKEGAMRIDARAGTHPVIQPIADALQAN
                     GAFDAITYSKGQAVIRMLENYLGEDAFRAGVRAYIKAHAYGNTVTDDLWAELDKTSAT
                     PISAVAHDFTMQAGVPLIRAAATQDGIRLTQDGLAADETGAAARAWQVPVVATTLDGK
                     SEWRGIVSAAQPVTIALAKGAVPIVNAGQAGYFRTLYDKTLSAKLAVHFRALAPADQL
                     GLLSDARALGTSGAQPLADLLALTSQARPDMNPQVLVKIANDLTDLGVHYRGLPGRAS
                     FRAYALGVLRPLFAKTGWAAQPGEDANVPLLRTALLNALNQLDDPKVIAKARQYFATY
                     VKSPASLSGDLRRSVLSIVAAHADAATWEQLHDLAKTAQSSLEKHQFYALLGSARDRA
                     LAERALDLALTDEAPVTMRPVIINRVAEHYPELAFAFTEAHLDAINAMLEPDSRNEFA
                     PRLAYSATDPAMIARLQAFAEAHIPATARQTTVKAQAAIAHAAKVRSQSLPEIDRWLK
                     TRNPHHR"
BASE COUNT          709 a         1253 c         1325 g          697 t
ORIGIN      
        1 gcgcgcttga cgcaaaacct ccgcccgccc gcttcgaccg cgaacacgtc gcaagacacg
       61 ccgcctgaca gggcgcgaat ggcgcaggat tcgtcctggc cgatcaatcc catgccgcgc
      121 aacgctgccg taaggtcgtc cacttcgccg ttcaacttca ttgctgcggt ctttcggctt
      181 gctcgcgcgg gttgccgccg cgtgccataa tcgcgcgcaa gaccatacca gaggaggaaa
      241 catgatccgc aactgtcttc tggccgcggc tgccacagcc atgctgagtg cgggcgcggg
      301 cgccgcgccg gtgcccgcca ctgtcaaagc cgccttgaac gatgccgcgc gccccgccga
      361 cgacgtcaag cgcgacgccg accgcaagcc tgccgcgatg ctcgccttcg ccggcatcaa
      421 accgggcgcg cgcgtgatgg atttgattcc gggcggaggc tatttcaccc gcatcttcgc
      481 caaggcggtc ggacccagcg gctatgtcta tgcgttccag ccgacggaac tcgaccactt
      541 cttcaaggac aaagagccgc ccatcgtggt ggtggcggcg gcatatccca acgtcagcgt
      601 catccatgcg ccggtcaaca cgctggcggc gccggagtcg ctcgacgtgg tgtggacctc
      661 gcagaattac cacgatctga agaacgactt cttcaaaccc gccgattccg cgctggtgaa
      721 caaggcggtc tacgacgcgc tcaagccggg cggcctctac atcgtgctcg accattcggc
      781 gccggagggt tcgggcgtgc gcgacaccga cacgctgcac cggatcgacg aagcggtggt
      841 gaagaaggaa gtggaagccg ccggattcaa gctggcgggc gaaagcaacg tcctgcgcaa
      901 tccgaaagat caacgcacgg tgaaagtgtt cgatccgtct atccgcggcc ataccgacca
      961 gttcatcctg aaattccgca agccggcggg gcacaaatga tgcgcgaagg aaaacatcgc
     1021 ggggtgctga tgatcggcgc tctgccggta ggaacggtcg tgatcgcggc gctggcattg
     1081 ggcgcgctgt ccatcggcgc gctcgccatc gccaggatgg cgatcgggcg cgtggcggtg
     1141 ggcaagctgc ggctcaaacg cgccgagatc ggtgaattga ccgtgcacac gctgcgcgtg
     1201 ctggacaaca agcctcatga acccgctcac ccttcgacgg gctcagggtg agggacttgt
     1261 tttaaccctc atgccgagct tgtcgaagca tgggcttgcc agccttcgct gcgctacagc
     1321 ggaccaaggg ttgatctctt gcccgccgga gctttagcgt aggcgggtcg aagcatgagc
     1381 ctgctcgccc atcaccggtg atgcgggttc cttgttttca gccagcggtc gatctcgggc
     1441 aggctttggg agcgcacctt ggcggcatgg gcgatggccg cttgggcctt caccgtcgtt
     1501 tggcgcgcgg tggccggaat atgcgcttcg gcgaaggctt ggagcctggc gatcatggcc
     1561 ggatcggtgg cactataggc cagccgcggc gcaaattcgt tgcggctgtc cggctccaac
     1621 atggcgttga tcgcatccag atgcgcttcg gtgaaggcga acgccagttc gggatagtgc
     1681 tccgccactc tgttgatgat cacgggccgc atggtgacgg gcgcttcgtc cgtcagcgcc
     1741 aggtccaggg cgcgttcggc cagagcgcgg tcgcgggcgc tccccagcaa ggcgtagaac
     1801 tggtgttttt cgagcgagct ttgcgcggtc ttcgccaagt cgtgcagttg ctcccaggtc
     1861 gcggcgtcgg catgagcggc gacgatggac agaacgctgc gccgcagatc gcccgacagg
     1921 cttgccggtg atttcacata ggtggcgaaa tattggcggg ccttggcgat gaccttggga
     1981 tcgtcgagct ggttcaaggc attcagcagg gccgtgcgca agagcggaac gttggcgtct
     2041 tcgccgggct gcgccgccca tccggtcttg gcgaagagcg gccgcaagac gccgagagcg
     2101 taggcgcgga acgaagcccg gccgggcagg ccgcgataat gcacacccag gtcggtgaga
     2161 tcattggcga tcttgacgag cacctgaggg ttcatgtcgg ggcgcgcctg actggtcagg
     2221 gccagaagat ccgccaacgg ctgagcgccg gacgtgccca gcgcacgggc gtcggagagc
     2281 aagccgagct gatcggcggg cgccaaggcg cggaaatgca cggcgagctt ggccgaaagg
     2341 gtcttgtcgt acagggtgcg gaaataaccc gcctggccgg cattgacgat cggcactgca
     2401 cctttggcga gcgcgatcgt gacgggttgc gccgcgctga cgatgccgcg ccactcgctc
     2461 ttgccgtcga gcgttgttgc gacgaccgga acctgccacg ccctcgccgc ggcgccggtt
     2521 tcgtcggcgg ccaaaccgtc ctgcgtgagg cggatgccgt cttgcgtcgc ggccgcgcgg
     2581 atcagcggca cgcccgcttg catcgtgaaa tcgtgcgcca cggcggaaat cggcgtcgcc
     2641 gaggtcttgt ccagctcggc ccacagatcg tcggtgacgg tattgccgta ggcgtgcgcc
     2701 ttgatgtagg cgcgcacgcc ggcccggaag gcgtcctcgc cgaggtaatt ctccagcatc
     2761 cggatcaccg cctggccttt cgaataggtg atcgcgtcga aggctccgtt ggcctgcaac
     2821 gcatcggcga tgggctggat gaccggatgg gtgccggcgc gcgcgtcgat gcgcatggcc
     2881 ccttccttcg aacccaggga cctgagccag gtgttccatt gtggattgaa gcggttcgtc
     2941 gccttgagct ccatccagga ggcgaaaccc tcgttcagcc agatgccgtc ccaccactcc
     3001 atggtgacga gatcgccgaa ccattgatgc gccatctcgt gagcgacggt gacatagacg
     3061 ccgcgcttgt cgctctcggt cgagattttc ggatcgatca gcagagtgcg ttcgaagaag
     3121 aacatggcgc cccagttctc catggcgccg aagaactggc tctggcccgg cccggcgatg
     3181 agatcgagct tgggcagcgg gtatttcacg ccgaaataat cttcgtaata gggaaggatc
     3241 tggaccgccg cgtcgagcgc gtattgggcc tgcgccgtat cgccgcgctt gacgatcacg
     3301 ccgatttcga cgccgtccac ttggcgcaca acgcgctcga agtcgcccag gccgaagaac
     3361 agaagatagg acgacatcac cggcgtttcg gcgaagcgca catgcgcgcg cccgtccgcc
     3421 agcatttccc gcgcggcgat gggcatgttg gacaccgcca tcgcgtccgc cgggaccgtc
     3481 gctgcgagta caaaactcgc tttccgggcc ggctcgtccc agcagggaac gaagcggcgg
     3541 gcgtcggaat tctcgaactg ggtgaacaac gcccgtttgg ttcccgcttc cgtgtcgtaa
     3601 tcgagataga acaagcccgc ggcgtgctgg ttgatcttgc cggcataatc gatggcaagc
     3661 acgtaacggc caggcgcgat cggcgcggcg aaggtgagcg tcgcggtttc ctttgttttg
     3721 tcgaaagcga cctgcggctg cccggcgacg ccggaaagcg ccacccggga gaacgtcaga
     3781 tcggcggcat tcagctcgat ccgcgaggtc tgttcgcgca cctcgatatc gatcttgacc
     3841 gcgcccgtga aggtcatggc cgccgcatcc ggcacgatcg aaagatcgta atgcgccggc
     3901 accacgtcgg cgggcagcac gacgcggtcg ggcgccgcct gcacggcttg ggggactaag
     3961 gcaagcagca acgcaaagac aaaa
//
LOCUS       DASWPU010000531         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_28724,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000531 DASWPU010000000
VERSION     DASWPU010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28724"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..282
                     /locus_tag="VGC27_11200"
     CDS             4..282
                     /locus_tag="VGC27_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY0283176.1"
                     /translation="MFQAVGLSRNESAQMVEDILEELCSALARGETVKLSSFGTFAVR
                     QKSQRMGRNPKTGDEVPIAPRRVLVFRPSHVLKAVINGQTPSAEAELD"
     gene            285..788
                     /locus_tag="VGC27_11205"
     CDS             285..788
                     /locus_tag="VGC27_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0283177.1"
                     /translation="MLAGAAQLAVHSKSPEAFRTISEVASELDVPQHVLRFWESRFAQ
                     VRPTKRAGGRRYYRPEDVDLLRGIRSLLYSEGYTIKGVQKLLRERGLRYVAQVGRGEA
                     VAEPAAIMQETEFDGEPASPRAVAEIVRLDGKSQALGEKERVKLEICLAELLRLKSRL
                     RAAAYRA"
     gene            987..1064
                     /locus_tag="VGC27_11210"
     tRNA            987..1064
                     /locus_tag="VGC27_11210"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1022..1024,aa:Pro,seq:ggg)
     gene            complement(1093..1617)
                     /locus_tag="VGC27_11215"
     CDS             complement(1093..1617)
                     /locus_tag="VGC27_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283178.1"
                     /translation="MNTIFDTRTFAKRNTIVVHDVGNCVKSWGLRGILLGGSFGFVAV
                     AILVALPLTTEVLTFGTIGTLIVGTIECAVVGGGFGVLAAALFGQGVLRRKTTGLART
                     LATDHQSAGADWPEGRIPLSEWPVRWSFPGASVTHSVLPILGDDWPTDAPLEQHDQKQ
                     INVIDIWENGSYGP"
     gene            complement(1833..>2128)
                     /locus_tag="VGC27_11220"
     CDS             complement(1833..>2128)
                     /locus_tag="VGC27_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283179.1"
                     /translation="VVQSFIRQSGDEIAKYGSGHRRDPTRQQHFATGSESTHMSLSIT
                     VKDRHPPPLCMTAYEHEVIGLCAGLSFTVSKYPLTYPQTENRQLGRNRLRLRE"
BASE COUNT          427 a          632 c          650 g          419 t
ORIGIN      
        1 gccgtgttcc aggcggtcgg tctgtcgcgc aacgaatccg cacagatggt tgaggatatt
       61 ttggaagagc tatgttccgc gctcgcccgc ggcgagaccg tgaagctctc ttccttcggc
      121 acctttgccg tgcggcagaa atcgcagcgc atgggccgca accccaagac cggcgatgaa
      181 gttcccattg ctccgcgccg cgtgcttgtg tttcgcccca gccatgtgct gaaggccgtc
      241 ataaacggac agacgccgtc cgccgaagcc gagctggatt gaagatgctc gccggcgcag
      301 cgcagcttgc cgttcactca aagtcgcccg aagcgttccg cacgatcagt gaggtcgcga
      361 gcgaattgga cgtgccgcag cacgttctgc gcttctggga gagccgtttc gcgcaagtgc
      421 gcccgaccaa gcgggcgggc gggcggcgct attaccggcc ggaagatgtc gatctgctgc
      481 gcggcattcg ctctttgctc tattccgaag gctacaccat caaaggcgtg caaaagctgt
      541 tgcgcgaacg cggccttcgc tatgtggcgc aggtcggccg cggagaggcc gtcgcggagc
      601 cggccgcgat catgcaagaa acggagtttg acggcgagcc tgcgtcgcca cgggccgtcg
      661 cggagattgt ccggctcgac ggcaagtcgc aggcgcttgg ggaaaaagag cgcgtgaagc
      721 tggaaatctg cctcgccgag ctgttgcggt tgaaaagccg cctgagagcc gccgcttacc
      781 gcgcctgaaa gtcgcgccat tgcgccttta tattgccgcc ctgagcttta cgcgcgcacg
      841 ggcgcggggg ataagtccgg gcaagacagg cagcagccaa aatcgtatgt tccgccgttg
      901 ccgcggggta tcccggcaac tatagtccgc cgcccttggg caacctttgc caaggaatac
      961 tccggcggca ttgccgcctg aagtgtcgga gcgtggcgca gcctggttag cgcacctgtc
     1021 tgggggacag ggggtcggag gttcaaatcc tctcgctccg accaactata ccaagttccg
     1081 gggttggggc cgtcacggcc cgtagctgcc gttttcccag atatcgatca catttatctg
     1141 cttctggtcg tgctgttcca aaggcgcgtc ggtaggccag tcatcgccaa gaatcggcag
     1201 aacagagtgc gtgaccgatg cgccgggaaa cgaccaccgg accggccact cggataatgg
     1261 aattctccct tccggccaat cggcgcctgc tgattggtgg tccgtggcaa gtgtccgcgc
     1321 caatcctgtt gttttacggc gaagaacacc ttggccgaag agcgccgcgg ccaacacgcc
     1381 gaacccgccg ccgacgaccg cgcattcgat agtgcctaca atcaaggtgc cgatggttcc
     1441 gaaggtgaga acttccgtgg tgagaggcag cgccacgagg atcgcgacgg caacaaagcc
     1501 gaagctgcct cccagcagaa tcccgcgcag accccaagac ttgacgcagt tgccgacatc
     1561 atgaacgacg atggtgttcc ttttggcaaa ggtccgagtg tcaaagatcg tgttcatttt
     1621 gcactctcca ctccggcgaa agcgatgctc ttgaagttgg gaagcatcgt tgcgtctcct
     1681 gaatccgacg cccgcccgcc gccgatacgg tggactgcac gggcctatcc tctactcgga
     1741 agcggctgtc ggcggccgat gcagctcgcg cggacttagg aaccaccacg atgttcatag
     1801 gctcggcttt aggccatgcg tagcctcgga atctactcac gcaaacgcag gcgatttcgg
     1861 ccaagctgcc ggttttccgt ctgtggataa gtcagtggat atttggaaac cgtaaaggat
     1921 agaccagcac ataaaccaat gacttcatgt tcatatgccg tcatgcacaa agggggaggg
     1981 tgcctgtcct taacagttat agacaaactc atatgagtag attccgatcc tgttgcgaag
     2041 tgctgctgcc gtgtagggtc acgtcgatgg cctgaaccat atttggcgat ttcgtcgcca
     2101 gattggcgaa taaagctctg aacgaccc
//
LOCUS       DASWPU010000532         6040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_17070,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000532 DASWPU010000000
VERSION     DASWPU010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17070"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1237)
                     /locus_tag="VGC27_11225"
     CDS             complement(107..1237)
                     /locus_tag="VGC27_11225"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY0283180.1"
                     /translation="MLCKRPYIRLCAAVFAAVLAALPQPLFASSRVKDLVEVEGIRDN
                     MLVGYGLVAGLNGTGDSLKNAPFTQQSIQTMLERLGVNTRGTIMQTKNVAAVMVTANL
                     RAFSAQGTRIDVSVSAMGDAKSLQGGTLLVTPLFGADGQIYALGQGPVAIGGFSAQGD
                     AASVTRGVPTAGRISNGAIVEREVGFALANERKLKLSLHNPDLTTATRIAAAVNAYLG
                     GSVAQASDPSTVHVNVPASYPNGVIGLLTDIEQVKVDPDLPAKVVIDAQSGVIVMGAN
                     VRISTVAIAQGNLTIHVNETPQVSQPAPFSNTGTTQVVPRTSIQIDDSKGNKMAVLHD
                     GVALQNLVNGLNALGVGPRDIISILQAIKAAGALQAEIQVIG"
     gene            1654..2415
                     /gene="gpmA"
                     /locus_tag="VGC27_11230"
     CDS             1654..2415
                     /gene="gpmA"
                     /locus_tag="VGC27_11230"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY0283181.1"
                     /translation="MADYKDYQLVILRHGESEWNKKNLFTGWRDVKLTEQGEREARSG
                     GCTLLQDGFAFDIAYTSLLTRAIKTLSLALEEMDLLWLPVIKHWRLNERHYGKLQGSN
                     KLEAVQKFGEDQVKIWRRSYATPPPPVDLSSPDYPKADRRYTGVPDADLPRSESLKDV
                     LGRVMPYWEEAILPELKAGKRILIAAHGNSLRALLKYLEGVSDEDIVNVDLPTGIPKA
                     YRLNAAYKADDARFLGDPQELERRIAAVKAQTKKA"
     gene            2441..3721
                     /locus_tag="VGC27_11235"
     CDS             2441..3721
                     /locus_tag="VGC27_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY0283182.1"
                     /translation="MRLKHVLTAFLVACAPSFAGADKVPVPRPRPAAARPAPPLQDGV
                     TDLSHAIPAKGLSKLPPTADQFQTLKQEIAKNQPEVATARKSSETLTRQAERLQRKLV
                     TMAARVEELEREKAQLGADIARLSAENARLSAGFARDRVAVSRLLAVLERLQHDMPPA
                     MALRPNDALAAARGAMLVGASLPNIYGEAAALARRIETLRNTRLALIARRAEAERNGA
                     YLAQARIELDQLLAMKRLEADAAVTHYGDLKAKLDTIASQAADLKSLLEKVAALRAVQ
                     ADQSVVTVTAEKGAGGTRLGRDLLVCPVVGEPVSGGMDGVGGAAAPGLTYATASGAQV
                     ISPADGRVVFAGPYHKSGQALILEMAGGYDAVLVGLDRLEVRLGNRVLAGEPVGMMSK
                     TNLRPRLYFELQLKGRGLSPAPYLSVTLRKAKRS"
     gene            3718..5016
                     /locus_tag="VGC27_11240"
     CDS             3718..5016
                     /locus_tag="VGC27_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0283183.1"
                     /translation="MKRLAFVGMGIVLGFIVALGVQPAAQGAATVSPYRQFNLFSEAF
                     ERVRANYVRPVQDSELIDGAIKGMVSSLDPHSSYMDAKSFADMQIQTKGEFGGIGIEV
                     TMEDGLVKVVSPIDDTPAAKAGIKTGDYIAAIDGNAIQGMSLNDALDKMRGPAGTKVT
                     LTIMRNGEKKPLDVALVRAVIHVDSVKWRREGDIGYIRLSAFNEATSSGLEKAVRELK
                     KQIGPGIKGYVLDLRNNPGGLLEQAVQVSDDFLSKGEIVSTRGRRADDTERYDARAGD
                     ITDGKPMIVLINAGTASASEIVAGALQDHKRARTLGMTSFGKGSVQTIIPLGEGGGAL
                     RLTTARYYTPSGRSIQAEGIVPDIAVAQSTEDSLPKIERPSEANLPGHLEAEAAAKKK
                     AAAVIRPAPGKKYDDFQLAYALDLLRGKMTVAVAKGEAVR"
     gene            5053..>6040
                     /locus_tag="VGC27_11245"
     CDS             5053..>6040
                     /locus_tag="VGC27_11245"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEY0283184.1"
                     /translation="MAASSMTGDLSPPRSQRRGAQALAFATLFVLVSLLALAGGIAVL
                     GNPHAGDQVASLELHLKRVARGAPPAAPPPAPPQTAALPPEAAPPADETQPAAPELPP
                     PVSAGRALVADPALIEKTPQGPLPRIADDGTPPMRAYAAPVVSAGKPRIAIVINGLGI
                     SAKATTAALAGLPAGVTLAFAPYANDVQRWVTEARQKGHEVLLEVPMEPYDFPDSDPG
                     QYTLRAGVGEDYNTRRLVWALTRFTGYTGVTNLLGGRFLSDSGALEPALTYLTRRGLL
                     FYDNGSAVHSVAPDVAARLGTHFAQATATIDSIRSAMEIDRRLSDLESAARTK"
BASE COUNT         1105 a         1932 c         1965 g         1038 t
ORIGIN      
        1 tcgaactgct ggccgacttt cttcgccgcg gccgcgtcgg cggtcgcttt cggcgtcatg
       61 accggacggt tctgcgcgag cgcgaccgcg ccttcgatct ccgcgttcat ccgatcacct
      121 ggatctcggc ctgcaacgcg ccggccgcct tgatcgcctg caggatggag atgatgtcgc
      181 gcgggccgac gccgagcgcg ttcagtccgt tgaccaggtt ttgcagcgcc acgccgtcgt
      241 gcagcacggc catcttgttg cctttgctgt cgtcgatctg gatcgaggtg cgcgggacaa
      301 cctgcgtcgt gccggtgttg gagaagggcg ccggctggct cacctgcggc gtctcgttga
      361 cgtggatggt cagattgccc tgggcgatgg cgaccgtcga aatccgcaca ttggcgccca
      421 tgacgatgac gccggactgt gcgtcgatga cgactttggc gggcaggtcg ggatcaacct
      481 tcacctgctc gatgtcggtc agcaggccga tgacgccgtt ggggtagctg gcggggacat
      541 tgacatgaac ggtagacggg tcgctggcct gcgcgacgga accgccaaga taggcgttca
      601 ccgcggccgc gatgcgcgtg gcggtcgtca gatcgggatt gtgcagggaa agtttcaatt
      661 tgcgttcgtt ggccagggcg aagccgactt cgcgttcgac gatcgccccg ttggaaatgc
      721 gccctgcggt cggcacgccg cgcgtcacgc tggcggcgtc gccctgggcg ctgaagccgc
      781 cgatggcgac cggaccctgg ccgagcgcgt agatctgtcc gtcggcgccg aacagcgggg
      841 tcaccagcag ggtgccgcct tgcagactct tggcgtcgcc catggcgctg accgaaacgt
      901 cgatgcgcgt gccctgggcc gaaaaggcgc gcagatttgc cgtcaccatc acagcagcca
      961 cgttcttggt ctgcatgatc gtgccgcggg tgttgacgcc cagccgctcg agcatggtct
     1021 ggatgctctg ttgggtgaaa ggcgcgttct tgagcgagtc gccggtgccg ttgaggccgg
     1081 ccaccaagcc atagccaacc agcatattgt cccggatgcc ttcgacctca accagatcct
     1141 tgacccgcga agaggcgaag agaggctgtg gcagcgcggc caggacggcg gcgaaaacgg
     1201 cggcacagag tcttatatac gggcgcttac agagcatggg cggttctgcc tgctgttttt
     1261 cggctttctc catgccagtt ttatgccgcc cggactaaac aaatagtaca atattatcag
     1321 atagttatag ttgtcccgcg ggtcctccaa gcatcactcg gcaggatttt ccccggcagt
     1381 tttgggcccg tcctactggg cgcatttcgg tcgaggtaga agaatctgcg aataactcgc
     1441 atattggccg cgccataggc gggcgaacgc cgttcggccg atgcgtcatg aaggcaaaat
     1501 agtgtcggcg ctggcacaac cgggttctct caggaagttc tcttccgttc tcaattcggc
     1561 cgcatttctg cggcgaaacg cggtgcaatc cgccgcggga aatgctgtag gttcgcgatt
     1621 cactgaacat ccgaaaggac gggaacgagc gacatggcag actataagga ctatcaattg
     1681 gtcattcttc gtcacggcga aagcgagtgg aacaagaaga atctgttcac cggctggcgc
     1741 gacgtaaaac tgaccgaaca gggcgagcgc gaagcgcgca gcggcgggtg cacgctgttg
     1801 caagacggct tcgctttcga catcgcctat acctcgcttt tgacccgcgc gatcaagacg
     1861 ctgtcgctcg ccctggaaga gatggacctg ttgtggctgc cggtgatcaa gcactggagg
     1921 ctcaacgaac gccattacgg caagctgcaa ggcagcaaca agctcgaagc ggtgcagaag
     1981 ttcggcgaag accaagtgaa gatctggcgc cgttcctatg cgacgccgcc gccgcccgtc
     2041 gatctcagtt cgccggacta tcccaaagcc gaccggcgct ataccggcgt gcccgatgcc
     2101 gacctgccgc gcagcgaaag tctcaaggac gtgctgggcc gcgtcatgcc ctattgggaa
     2161 gaggctatcc tgccggaact caaggccggc aagcgcatcc tcatcgccgc ccacggcaat
     2221 tcgttgcgcg ccctgctcaa atatctcgag ggtgtgagcg acgaggatat cgtcaatgtc
     2281 gatctgccga ccgggattcc caaggcctat cggctgaacg cggcctacaa agccgacgac
     2341 gcgagattcc tcggcgaccc gcaggagctc gaacggcgca ttgccgccgt caaggcccag
     2401 acgaaaaagg cgtgacgttg cgctataacg gcgcatgaag atgcggctaa agcacgtcct
     2461 taccgcgttc cttgtcgctt gcgccccgtc ttttgccggg gcggacaagg tgcctgtgcc
     2521 gcgtccaagg cccgcggccg cgcgccccgc gccgccgctg caggacggag tgaccgatct
     2581 cagccatgcg attccggcca aggggctgtc caaactgccc cccaccgcgg atcagttcca
     2641 gacgctgaag caagagatcg ccaagaacca acctgaagtc gcgaccgcca ggaaatccag
     2701 cgaaacgctt acccgccaag ccgagaggct acagcgcaaa ttggtgacga tggcggcgcg
     2761 cgtcgaagaa ctggagcggg agaaggctca gcttggcgcc gacatcgccc gcctgagcgc
     2821 ggagaatgcg cgcctgtccg ccgggttcgc gcgcgatcgc gttgcggtgt cgcggctgct
     2881 tgcggtgctg gagcggttgc agcacgacat gccgccggcc atggcgctga ggccgaacga
     2941 cgccttggcg gcggcgcgcg gcgccatgct ggtcggcgcc tccttgccga acatctatgg
     3001 cgaagcggcg gcgcttgccc gccgcatcga aacgctgcgc aataccaggc tggccttgat
     3061 cgcgcgccgc gccgaagccg aacgcaatgg ggcctatctg gcgcaagcgc gcatcgaact
     3121 cgatcaactt ctggcgatga aacggttgga agccgacgcc gccgtgaccc attacggcga
     3181 tctcaaggcc aagctcgaca cgattgcttc gcaggccgcc gatctgaagt cgctgttgga
     3241 aaaagtggcg gccttgcggg ccgtccaggc cgaccaaagt gtcgttacgg taactgccga
     3301 aaaaggcgcc ggcggcacga ggctggggcg ggacttgctg gtttgtccgg tggtcgggga
     3361 gccggtttcc ggcggaatgg atggggtggg cggggctgcc gcgccgggcc tgacctacgc
     3421 cacggcgtcc ggcgcgcagg tcatttcgcc ggccgacgga agggtcgtat tcgccggtcc
     3481 ttaccataag agcggccaag ccttgatctt ggaaatggca ggcggctacg atgctgtatt
     3541 ggtagggctg gatcggcttg aggtccgtct cggtaatcgc gtgctcgcgg gagagccggt
     3601 cgggatgatg tcaaagacca acctgcggcc tcggctttat ttcgaattgc agctgaaagg
     3661 ccggggactc agcccggcgc cctacctgtc ggtcacgttg aggaaggcaa agagatcatg
     3721 aaacgcctcg ctttcgtcgg catgggcata gttctcggct tcattgtcgc tctcggcgtg
     3781 cagccggcgg cgcagggtgc cgcgacggtc agcccctatc gccagttcaa tctcttcagc
     3841 gaagccttcg agcgcgtccg cgccaactat gtgcggccgg tccaggacag cgagttgatc
     3901 gacggcgcca tcaagggaat ggtgtccagt ctcgatcctc actccagcta catggacgcg
     3961 aagagtttcg ccgacatgca gatccagacc aagggcgagt tcggcggcat cggcatcgaa
     4021 gtaacgatgg aagacggcct ggtcaaagtg gtctcgccga tcgacgacac gcccgccgcc
     4081 aaggctggaa tcaagaccgg cgattacatc gccgccatcg acggcaacgc catccagggc
     4141 atgtcgctga acgatgccct cgacaagatg cgcggccccg ccggcaccaa ggtgacgctg
     4201 acgatcatgc gcaacggcga gaagaagccc ctcgacgtgg cactggtgcg cgcggtcatc
     4261 catgtcgaca gcgtcaaatg gcgtcgcgag ggcgacatcg gctatatccg gctcagcgcc
     4321 ttcaacgagg cgacgtcgag cggcctggag aaggccgtgc gcgaactcaa gaagcagatc
     4381 ggcccgggga tcaagggtta cgtgctcgat ttgcgcaaca atccgggcgg cttgctcgaa
     4441 caggccgtcc aggtgtccga cgatttcctc agcaaaggcg agatcgtctc cacccgcggc
     4501 cgccgtgcgg acgataccga acgctacgac gccagggccg gcgatatcac cgacggcaag
     4561 ccgatgatcg tgctgatcaa cgcgggcacc gcttcggcct ccgaaattgt cgccggcgcg
     4621 ctgcaagacc acaaacgcgc caggacgctg ggcatgacct ccttcggcaa gggatcggtg
     4681 cagaccatca tcccgctcgg cgaaggcggc ggcgcgctca ggctgacgac ggcgcgttac
     4741 tacacgccgt cgggccgttc gatccaggcc gagggcatcg ttcccgacat cgccgtggcg
     4801 cagagcaccg aggacagcct gcccaagatc gaacgcccca gcgaggccaa tctgcccggt
     4861 catctggagg ccgaagcggc ggcgaagaag aaggccgccg ccgtgatccg tcccgctccc
     4921 ggcaagaaat acgacgactt ccagcttgcc tatgcgctcg atctcttgcg cggcaagatg
     4981 acggtcgccg tggccaaggg cgaagccgtg cgttaaccgg ggcgtaacca tgttgaccct
     5041 tagctgaggg ccatggctgc gagcagtatg accggggacc tgtccccgcc gagaagccaa
     5101 cgccgcgggg cgcaggcgct ggcgtttgcc accctttttg tccttgtctc gctgctggcg
     5161 cttgccggcg gaatcgctgt cttgggcaat ccccatgcgg gcgatcaggt cgcatccctg
     5221 gagctgcatc tgaaacgcgt ggcgcgcggc gcaccacctg ccgcgccgcc tcccgccccg
     5281 ccacaaaccg ccgctctgcc gcctgaagcc gcgccgccgg ccgacgaaac ccagcccgcc
     5341 gcacccgaac tgccgccgcc ggtttcagcg ggacgggcgc tcgttgccga tccggctttg
     5401 atcgaaaaga cgccgcaagg tcccttgccg cggatcgccg acgacggaac gccgcccatg
     5461 cgcgcctatg cggcgccggt ggtctcggcg ggtaagccgc gcatcgccat cgtcatcaac
     5521 ggactcggca tcagcgccaa agcgacgacg gcggcgttgg ccggtctgcc tgcgggcgtc
     5581 acgctcgcct tcgcgcccta tgccaacgac gttcagcgct gggtcaccga ggcgcgccag
     5641 aaggggcatg aagtgctcct cgaagtgccg atggagccgt acgattttcc cgacagcgat
     5701 ccgggccaat acacgctgcg cgccggcgtc ggcgaagact acaacaccag acgcctggtg
     5761 tgggcgctga cgcgcttcac cggctatacc ggcgtgacca atctcctcgg cgggcggttt
     5821 ctatcggatt ccggcgcgct ggaaccggcg ctgacctatc tgaccaggcg cgggcttctg
     5881 ttctacgaca acggctcggc cgtgcattcg gtggcgccgg acgtcgccgc gcgcctcggc
     5941 acccatttcg cccaggccac cgccacgatc gactcgatcc gatcggccat ggagatcgac
     6001 cgtcggcttt ccgacttgga atccgcggcg cgcaccaagg
//
LOCUS       DASWPU010000533         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_53379,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000533 DASWPU010000000
VERSION     DASWPU010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53379"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(517..1248)
                     /locus_tag="VGC27_11250"
     CDS             complement(517..1248)
                     /locus_tag="VGC27_11250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0283185.1"
                     /translation="MARLQGKVVLITGASAGIGQASAKALAREGAKLVLTARRKDRLE
                     QLAAEAKKLGSEAVYVIGDAREEETAKQAVAAAQKAFGRIDILIANAGVGNYKNLVDT
                     SAAEYDEMMDSNVRTSWLFARHVAPVMIAQKDGLILFVSSMAGVYGFAGEATYCATKF
                     AQVGFAQGLDKELRPHGIKVGTVNPGGVKTEFAIGKGRSEQGVAQSEMLEAEDVAQAI
                     LFACTQPKGARIIQIQMRTMAEALT"
     gene            complement(1278..1430)
                     /locus_tag="VGC27_11255"
     CDS             complement(1278..1430)
                     /locus_tag="VGC27_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283186.1"
                     /translation="MGTAVESTLEVLDLATCARHTVYRTAGRIEAPNWTPGGRQFAFV
                     SYRLAG"
     gene            complement(1532..1732)
                     /locus_tag="VGC27_11260"
     CDS             complement(1532..1732)
                     /locus_tag="VGC27_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283187.1"
                     /translation="MGRIGQIALGALVAVGIYGTSVGMESPQLDARQPEFAARQLERL
                     YVDAKIADVTQSIIRLLLNILD"
BASE COUNT          400 a          623 c          603 g          413 t
ORIGIN      
        1 atctcaagct gatcccgaac ccgaagttgc agcgcaagac cgtggaacgg ctggaaaaga
       61 tcaaacaggg cgagcgggac ttttatttct agagtcgtat gccatcaagt cgatgcgcaa
      121 aaaacttgtt gtcatggccc ggttcatagc gacagcgtat gaacgcgagc cacccaggtg
      181 acgacggtac gacgccaatg gaacttcgct gagtctcttc ttcgacaatc tcaccacgtt
      241 tcaactggat ggcccgcagt cgcgggccat gacaagcgga tatttgattc cgtccgatca
      301 caagcggttc tagagcgcaa tccggcaaag tggtcggtag cagcagcgtg ccgacgcggt
      361 ttgccgttag attgcgcgaa aaataagaaa tcgagagtgt gatccaattc aattaaatcg
      421 gatcacactc tagcgcaccg ctggaaagat caggtcaatg attccgccat tcggcttcgc
      481 tgaagctgcg ccggacaagc cgtggaggcg gaaagatcag gtcaatgcct cagccatggt
      541 ccgcatctgg atctggatga tgcgcgcgcc cttgggctgg gtgcaggcga acaggatcgc
      601 ctgggcgacg tcttcggctt ccagcatctc ggactgcgcg acgccttgtt cgctgcggcc
      661 tttgccgatg gcgaactcgg tcttgacgcc gccgggattg acggtgccga ccttgatgcc
      721 gtgcggccgc aactccttgt cgagcccctg ggcgaagccg acctgggcga acttggtcgc
      781 gcaataggtg gcctcgccgg caaaaccgta gacgccggcc atcgaggaaa cgaacaggat
      841 cagcccgtcc ttctgcgcga tcatcaccgg cgccacatgg cgcgcgaaca gccagctcgt
      901 gcggacgttg gaatccatca tctcgtcata ttcggcggcg ctggtgtcga ccaggttctt
      961 gtagttgccc acgccggcat tggcgatcag gatatcgatg cggccgaagg ccttttgcgc
     1021 cgccgcgacc gcctgcttgg cggtttcttc ctcgcgcgcg tcgccgatca catagaccgc
     1081 ttcgctgccg agcttcttgg cttcggcggc gagctgctcc agccgatcct tgcgccgcgc
     1141 cgtcagcacc agctttgccc cttcgcgcgc cagcgccttc gcggaagcct gcccgatgcc
     1201 cgcgctcgcc ccggtgatga gaactacctt accctgcaac cgtgccatga cgccttcctt
     1261 ttcgagtttt gcggtactta ccccgccagc cggtagctga caaaggcgaa ctgcctgccg
     1321 ccgggcgtcc agttgggcgc ttcgatgcga ccggcggtgc ggtagaccgt gtgccgcgcg
     1381 caggtggcga ggtccaggac ttcaagcgtg ctttcgaccg cggttcccat ttttccgtcc
     1441 ccatgcccgc gcggcagcgt agcttgagtc ggcgcttccg gcagaagagg ctagaggtcc
     1501 cgcgccggcc gatcacaaaa gccgaaggag cctaatcgag gatgttcaac agcaaccgaa
     1561 tgatggattg cgtcacatcg gcgattttcg cgtcgacata cagacgttcc agttgcctgg
     1621 ctgcgaattc cggttggcgg gcgtcaagct gcgggctttc catgccgacg gatgtgccgt
     1681 aaatgccgac ggccaccaat gccccgagtg caatctgacc aatcctgccc atgtgaatac
     1741 ccaagcggcc gacggactac agaccacata gtaattgtgg cttttttccg aaaacctcgc
     1801 tgcctcggcg gccgcagtcg gagtaaaaaa ttggtgatcc gcggccgggt gtaagtccaa
     1861 ggcaggtcct ggccatgttg cgacaagctg aataattgac tcctaagccg ttttgtccga
     1921 atacgtgaaa ttgagagtgt gtcgcaacaa tgggccgcct tttatgagcc gtgacagcca
     1981 gattgccgct tccaaactcc acactcggct tggccgcgca gtggccggat tccgcggcc
//
LOCUS       DASWPU010000534         6347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_115219,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000534 DASWPU010000000
VERSION     DASWPU010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115219"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VGC27_11265"
     CDS             <1..528
                     /locus_tag="VGC27_11265"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEY0283188.1"
                     /translation="ETDLPPAALSKLADYMRSGGTILIDTRDLTLGAVRGVNSPGQQT
                     LRRLLGKLDLPPLEVLPPDHVLTKTFYLLSDFPGRWDGAPVWVQALPPGNPGEGPVRG
                     GDSVSPVIIGGNDWAAAWAVDGQGRPLADVSPGGERQRELAIRFGVNVVMYALTGNYK
                     TDQVHLPAILERLGK"
     gene            533..2623
                     /locus_tag="VGC27_11270"
     CDS             533..2623
                     /locus_tag="VGC27_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283189.1"
                     /translation="MGMLAFAPHLAPGLLWALIAAASILAGYAFFARARGAWARALAL
                     LVLILALANPLMVRQSREALSDVVAIVVDHSQSMDIGSRLADADKAADELKKRLAGEK
                     NLEVRVVTVTTNASGEDNGTQAFAALNAALADVPPSRVAGGILITDGEVHDAPAKPGA
                     ALPLQALIVGRRGEQDRKLTVVDAARYAIVGQKAQIVVRVDDLGGGKGGFARVGLRID
                     GTDAGTLSVPLGRNTPVSVPITHEGESVVEIEAEPGPAELTLQNNRAVAAVSGVRDRL
                     RVLLISGEPNAGERVWRNLLKGDPSVDLVHFTILRPPEKQDETPLKELSLIVFPTQEL
                     FAEKLDGFDLVIFDRYSEHGILPLAYFENIASYVYNGGALLVSAGPEFAGGLSIYRTP
                     LSSVLPAQPSGEILTQPFKPLVTPEGLAHPVTRDLPGANTDKTPPAWGRWFRLIGAAK
                     ITGATVMSGPGDKPLLVLDRTGKGRVAELLSDQGWLWARGFDGGGPEAELLRRLAHWL
                     MKEPELEEERLTAAISGGDIVIERRTMAAAPPPVTLTDPSGKQSAVALSRVEPGVWRG
                     QAKANALGLYRVTDGKFAAVAAAGPLNPKEVADMRATDTILKPVAEQSGGSVHWLADG
                     MPAIRRVERGHSMAGGHGMAGGRRMAGEDWIGIAANGASRITSVEQTPLLPAWAALLL
                     LLGTLMLAWRQEGR"
     gene            complement(2782..3330)
                     /locus_tag="VGC27_11275"
     CDS             complement(2782..3330)
                     /locus_tag="VGC27_11275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY0283190.1"
                     /translation="MKANEQPAWEIRPERPEDAPLVEALNEASFGPGRFAKSAYRLRE
                     GVDPVAGLSFVAIEAGELRGSLRFWPVAIGMEKALLLGPLAVQSDQRGRGIGIALMRR
                     GIEEARAQGHASIILVGDELYYVRVGFAALPPGRIHFPGPVDPARILGLSLHHNVMLT
                     LKGEVRRSHIDHPVCADGAKTG"
     gene            complement(3384..3842)
                     /locus_tag="VGC27_11280"
     CDS             complement(3384..3842)
                     /locus_tag="VGC27_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0283191.1"
                     /translation="MLSLFRKMLFQLTVLLRAFLSPVDFGALALVEQGGRLVLVRHSY
                     AAGWHLPGGGVRRGEPPEAAMLRELKEELGLAWASEPEFVGIFTRKVGLASNVIALYR
                     VREARFDFKPSLEVREIVLADPASPPPGTAGGVRRRLAELTGQTPQSPYW"
     gene            4049..4564
                     /locus_tag="VGC27_11285"
     CDS             4049..4564
                     /locus_tag="VGC27_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0283192.1"
                     /translation="MPGATFEHLRALIVEDNVHMRILLRSLLSALGITRIYEADNGAE
                     AFALMHENSPDLILTDLSMMPVDGITFTREIRLSHDSPNPYVPIIMITGHTERHKVEA
                     ARDAGVTEFLAKPITAQGLFLRIAEIVERPRPYVRCADYFGPDRRRRADEHFAGPWRR
                     HEDVANDAEFR"
     gene            4561..5052
                     /locus_tag="VGC27_11290"
     CDS             4561..5052
                     /locus_tag="VGC27_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283193.1"
                     /translation="MTGKPPASPPKHARATKIMPGAYPVFPRKTAEGLLQALRNPEVI
                     SAAARVVADRAEELRVAVLAHVDRIADAAHAADPAAIYAQAHEIRGLAGNAGLLAAGR
                     IANGLCRYLDALGRSGRQPDQELVVLHLEAIARAARAEDEATRLGDTVASELAALVDK
                     KLA"
     gene            5168..6043
                     /gene="motA"
                     /locus_tag="VGC27_11295"
     CDS             5168..6043
                     /gene="motA"
                     /locus_tag="VGC27_11295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEY0283194.1"
                     /translation="MLQIGGLVVLFVCVFGGFLISGGSLGVVLEAVPHEVLTIGGAAT
                     AALLIGGSMYTLKKMGGDIGKIISGPKWKPGDYRDLLCLLYLLAKTMKTKGLIALEAH
                     IERPEESSIFRRYPKIGKDRFALSFICDTLRMMTMSLEDPHQVESAMEKQLEKHHHEA
                     LATANALQTMADGLPALGIVAAVLGVIKTMGAISEPPEVLGRMIGSALVGTFMGVFLA
                     YGIVGPISVRLRSIIDEEALFYHVIRDILVAHLHGNAAQISVEIGRGSVPSEAQPTFL
                     ELEEALNNIPPEHHA"
BASE COUNT         1175 a         2065 c         2020 g         1087 t
ORIGIN      
        1 gaaacggatt tgccgccggc ggcgctgtcg aaactcgccg attacatgcg cagcggcggc
       61 accatcctga tcgacacccg cgatctcacc ctcggcgccg tgcgcggcgt gaactcgccc
      121 ggccagcaga ccttgcgccg gctgctcggc aagctcgatc tgccgccgct ggaggtgctg
      181 ccgcccgatc atgtgctgac caagaccttc tatctgctct cggatttccc gggccgctgg
      241 gatggagcgc cggtgtgggt gcaggctttg ccgccgggca atcccggcga agggccggtg
      301 cgcggcggcg acagcgtctc gcccgtgatc atcggcggca acgactgggc cgcggcctgg
      361 gcggttgacg gacaaggaag gccgctggcc gacgtttctc ccggcggcga gcgccagcgc
      421 gaactggcga tccgcttcgg cgtcaatgtg gtgatgtacg cgctcaccgg caactacaag
      481 accgaccagg tgcacctgcc cgccatcctc gaacggttgg ggaaataggc gcatgggcat
      541 gctcgccttc gctccgcacc ttgctcccgg cctgctctgg gcgctgatcg ccgccgcttc
      601 catcttggcc ggttacgctt ttttcgcgcg cgcgcgcggc gcctgggcgc gggcactggc
      661 gttgctggtg ctgatcttgg ccctcgccaa tccgttgatg gtccgccaga gccgcgaagc
      721 cttgtcggac gtcgtggcga tcgtggtgga tcattcacag agtatggata tcggctcgcg
      781 cctcgccgac gccgacaagg ccgccgatga actcaagaag agactcgccg gcgaaaagaa
      841 tctggaggtg cgcgtcgtca ccgtcacgac caacgcctcc ggcgaggaca atggtaccca
      901 agccttcgcg gccttgaacg cggcgctggc cgacgtgccg ccgtcgcgcg tggcgggggg
      961 aattctgatc accgacggcg aagtgcatga cgctccggcc aagccgggcg ccgccctacc
     1021 gctgcaggcg ctgatcgtgg gacggcgcgg cgagcaagac cgcaagctca cggtggtgga
     1081 cgcggcgcgc tacgccatcg tcggacagaa ggcgcagatc gtcgtccgtg tcgacgatct
     1141 gggcggcggc aagggcggtt tcgcgcgcgt cggcctgcgc atagacggca ccgatgccgg
     1201 gacgctgagc gttccgctcg gccgcaacac gcccgtctcg gtgccgatca cccacgaagg
     1261 cgaaagcgtc gtcgagatcg aagccgaacc gggtcccgcg gaactgacgc tgcagaacaa
     1321 tcgcgcggtg gcggcggtga gcggcgtgcg cgaccgcctg cgggtgctgc tgatctcggg
     1381 cgaacccaat gccggcgagc gggtgtggcg caacctgctc aagggcgatc cctcggtcga
     1441 tctggtgcat ttcacgattc tgcgcccgcc ggagaagcag gacgaaaccc cgctcaagga
     1501 attgtctctg atcgtcttcc cgacccagga actgttcgcc gaaaagctcg acggcttcga
     1561 cctggtgatc ttcgaccgct acagcgagca cggcatcctg ccgctggctt atttcgaaaa
     1621 catcgcgagc tatgtctaca atggcggcgc gctcctggta tcggccggac cggaattcgc
     1681 cggagggctg agcatctacc gcacgccgct gtcctcggtg ctgccggcgc agcccagcgg
     1741 cgagatcctc acgcagccct tcaagcctct ggtcacgccg gaaggcctcg cccatccggt
     1801 gacgcgcgat ctgcccggcg ccaacaccga caagacgccg ccggcctggg gccgctggtt
     1861 ccgcctgatc ggcgcggcga agatcacggg cgcgaccgta atgagcggac ccggcgacaa
     1921 gccgctcttg gtgctcgacc gcaccggcaa aggccgcgtg gcggaactgt tgtcggatca
     1981 gggctggctg tgggcgcgcg gcttcgacgg cggcgggccg gaagccgaat tgctgcgccg
     2041 ccttgcgcat tggctgatga aggaaccgga actcgaagag gaaagactga ccgccgcgat
     2101 ttccggcggc gacatcgtca tcgagcgccg caccatggcg gctgcgccgc cgcccgtgac
     2161 gctgaccgat ccatcgggca agcaatcggc tgtcgcgctg tcgagggtcg agcccggcgt
     2221 ctggcgcgga caagccaaag cgaacgcgct gggcctttat cgcgtcaccg acggcaaatt
     2281 cgccgccgtc gcggcggcag gaccgctcaa tccgaaagaa gtcgccgaca tgcgcgcgac
     2341 cgatacgatc ctaaagccgg tggccgaaca aagcggcggt tcggtgcatt ggctggccga
     2401 cggcatgccg gccatccgcc gcgtcgaacg cggccatagc atggcgggcg gccatggcat
     2461 ggcgggcggc cgtcgcatgg cgggtgagga ctggatcggt atcgccgcca acggggcctc
     2521 gcggattacc agcgtcgaac agacgcctct gttgcccgct tgggcggcgc tgctgctgtt
     2581 gctcggaaca ttgatgctgg cttggcggca ggagggccgc tagtgtcgtg gtttcgaagt
     2641 tcgcaccatt cgttacatcg ggctggaagc gaacttcgaa accaaaacga cactaggatt
     2701 caataggttg ctagtgtcct ttcgattcag aaattcgccc gaagccgata tagagagggt
     2761 gcgaatttcg gaatcgggac actagcccgt cttcgcccca tcggcacaga ccggatgatc
     2821 gatatgggag cggcgcactt cgcccttgag cgtcagcatc acattgtgat ggagcgagag
     2881 gcccagaatt cgcgccggat cgaccggccc cggaaagtgg atgcgccccg gcggcagcgc
     2941 cgcgaatccc acgcgcacat aataaagctc atcgccgacc aggatgatgc tggcgtggcc
     3001 ctgggcgcgc gcttcctcga tgccccgccg catcagcgcg atgccgatgc cgcgtccgcg
     3061 ctggtcggac tgcaccgcca aaggccccag cagcagggct ttttccatgc cgatggcgac
     3121 cggccagaag cggagcgagc cgcgcaattc gcccgcctcg atggcgacga aactgagccc
     3181 ggcgaccgga tccacgccct cgcgcaacct atatgccgat ttggcaaagc ggccgggacc
     3241 gaaactcgcc tcgttcagcg cctccaccaa aggggcgtct tccggacgtt cgggtctgat
     3301 ctcccaggcg ggctgctcat tcgccttcat gatctctggc taatgtcggc gcagggcaaa
     3361 agcaaggcgg ggaagacaaa cgatcaccaa tacggacttt gcggcgtttg tccggtgagt
     3421 tccgccagcc gccgacggac gccgcccgcc gtgccgggcg gcggcgacgc gggatcggcc
     3481 agcacgattt cgcgtacctc gaggctgggc ttgaagtcga agcgtgcctc gcgcacgcgg
     3541 taaagcgcga tgacattgct tgccagcccg accttgcgcg tgaaaatccc gacgaattcc
     3601 ggctcgctcg cccaggcgag gccgagttcc tctttcagtt cgcgcaacat ggccgcctca
     3661 ggaggctcgc cacgccgcac gccgcctccg ggaagatgcc atccggccgc ataggaatgg
     3721 cggaccagga caagcctgcc cccctgctcc accaaagcga gggccccgaa atcaaccgga
     3781 gatagaaaag ccctcagcag aacggtgagc tggaaaagca tttttctgaa aagacttagc
     3841 atgggatggc gaaatttcct ttcctgggga caacttcata aggatttgtt aaccatcctt
     3901 acctaacttt agatagcatt gcggtgcgaa ccatgccgtc ggctggagcc ttttacacgc
     3961 gctgcagcgc gccagcacga cacaacgcga tatatcggca tcggcggtat acccggatgt
     4021 gtcgatcctt ttctcgggct gaggggcgat gccgggggca acattcgaac atctgagggc
     4081 gctcatcgtc gaggacaatg tgcatatgcg catattgttg cgctcgctgt tgtcggcgct
     4141 cggcattact cgcatctacg aggccgacaa cggcgccgag gcattcgccc tcatgcacga
     4201 aaattcgccg gacttgatcc tgaccgatct gtccatgatg ccggtggacg gcatcacctt
     4261 cacgcgcgaa atacggttgt cgcacgacag tcctaatccc tacgtgccga tcatcatgat
     4321 caccggccat accgagcgcc acaaggtcga ggccgcgcgt gacgcgggcg tcaccgaatt
     4381 cctggccaag ccgatcacgg cgcagggcct gttcctcagg atcgcggaaa tcgtcgaacg
     4441 gccgcgcccc tatgtgcgtt gcgccgacta ttttgggccc gatcgcaggc gccgcgcgga
     4501 cgagcacttc gccggcccct ggcgccggca cgaggacgtg gcaaacgacg cggaattcag
     4561 atgaccggca agcctcccgc gtctccgccc aaacacgccc gcgcgaccaa gatcatgccg
     4621 ggcgcttatc cggtgttccc gcgcaagacg gcggaggggc tgttgcaggc tttgcgcaat
     4681 cccgaggtga tctccgccgc ggcgcgggtc gtcgccgaca gggcggaaga gttgcgcgtt
     4741 gcggtgctcg cccatgtcga tcgcatcgca gacgcggcgc acgccgccga tccggccgca
     4801 atctatgcgc aggcgcatga aatccgcggc ctggcgggaa acgccggact tctcgccgcc
     4861 ggacgcatcg ccaacggatt gtgcagatac ctggacgcgc ttggccgttc cggacgccag
     4921 cccgaccaag aactggtggt gttgcacctt gaggccattg cgcgggcagc gcgtgccgaa
     4981 gacgaagcga cgcggcttgg cgacacggtc gccagcgagc tggccgccct ggtcgacaag
     5041 aagctggcgt gaattaacga ttcggtaaga aacgtaaaca gtgccgcaag cccccgccgg
     5101 cgccgcccga tttatcgctc tttaaaccct ttcctcaata ctctcccccg ctatacgggc
     5161 gggttgaatg cttcaaatcg gcggacttgt cgtccttttc gtctgcgtgt tcggcggatt
     5221 cctcatttcg ggcgggtcgc tcggcgtggt gctcgaagcc gttccgcatg aagtcctgac
     5281 catcggcggc gccgccaccg cggctttgct gatcggcggc tcgatgtaca cgctgaagaa
     5341 gatgggcggc gacatcggca agatcatctc cggaccgaaa tggaaaccgg gcgattaccg
     5401 cgatctgctc tgcctgttgt acctgctcgc caagacgatg aagaccaagg gcctgatcgc
     5461 gctcgaagcc catatcgaac ggccggaaga atcttcgatc ttcagacgct atcccaagat
     5521 cggcaaggat cgttttgccc tctccttcat ttgcgacacg ctgcgcatga tgaccatgag
     5581 ccttgaagac ccccatcagg tcgagtcggc catggagaag caactcgaga aacatcacca
     5641 cgaggcgctg gccacagcca atgcgctgca aacgatggcc gatgggctgc ccgcgctcgg
     5701 catcgtcgcc gccgtgctcg gcgtcatcaa aaccatgggt gcgatttcag agccgccgga
     5761 agtgctcggc cgcatgatcg gctcggcgct ggtcggcacc ttcatgggcg tgttcctggc
     5821 ctatggcatc gtcggcccaa tcagcgtgcg gctgagatcg atcatcgacg aggaggccct
     5881 gttctatcac gtcatccgcg acatcctggt ggcgcatctg cacggcaacg cggcgcagat
     5941 ttcggtcgag atcggacgcg gcagcgtgcc ctcggaagcc cagccgactt tcctggaatt
     6001 ggaagaagcg ctcaacaaca tcccgccgga acatcacgcc tagagcgcaa tccggcaaag
     6061 tgtgaagcgg tttgccgtca gattgcgcga aatcaaggaa tctagagctt gatccgattc
     6121 aatcaaatcg gatcaagctc taggcgaaga acaggcagga aaccttgttc gctatttgcg
     6181 gaccggcgcc gttccgcgct tctccgccag cgccacgaag aacgtcgcca tcgcgccggc
     6241 caaaacgccg agcggcaccg cgataaatac gtccacatcc gttccgaaca cgacgattgc
     6301 ggtgacgatc atgcccgtca gttgcgccac tttcggcatg cctcgaa
//
LOCUS       DASWPU010000535         5665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_121278,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000535 DASWPU010000000
VERSION     DASWPU010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121278"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VGC27_11300"
     CDS             complement(<1..533)
                     /locus_tag="VGC27_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0283195.1"
                     /translation="MIAPKSLARQAMERDGNKPGHDCPSAPGPFASGGRMPLGDCKMR
                     ILLIEDNAAQASGIKPVLRSEGFDFNSSEIDKEGVELGKRCDYDVIILDRSLSDMNGL
                     NVLKALRAAKVHAPVLVLSGDASTRAMVSSLDAGADDYITKPYHKAELVARICAVARR
                     SRVHPQSLIAEGKIILNL"
     gene            complement(649..1272)
                     /locus_tag="VGC27_11305"
     CDS             complement(649..1272)
                     /locus_tag="VGC27_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0283196.1"
                     /translation="MSTEPVIYVVDDNALLRDSMRTLLECAGYIVRDYPSAKLFLADR
                     VYHSGCVIVDICMPEMNGLEMQEEIVRRGIDLPVIVITGHGDVPLAVRAMKAGAVEFL
                     EKVFKCERLLESVQRALATGEKARAEAEELTTAKHLLALLTPREHGVLDQLVAGHSNK
                     IAAYELGISPRTIEIHRAHIMDKLNARNLSDVVRIALTASWTQKDAA"
     gene            complement(1269..2765)
                     /locus_tag="VGC27_11310"
     CDS             complement(1269..2765)
                     /locus_tag="VGC27_11310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0283197.1"
                     /translation="MIEKARAAKRSEIRNTVAPDNDALLSALISSAADGILVTNAQGM
                     VQIYSGACEEIFGYPKNEVVGRHAKMLMPHYGERGDCPFFDSKISAMGIAKSGETVGR
                     RKDNSIFPVQFSVGEGELHDRRIFVTILRELTDDRNAGDAIKKREIRLRSILEAAPYA
                     IITIDENCLIETFGPAASQLFGYAPDEAIGRDVKILMPQTCHDHQEGYRIVVGHHRDG
                     STFPLELTVVELQGGSKVLFSSFVHGTNGRHDAQKRLYELQAELLHVSRLSAMAQMSF
                     AIARKLTEPLTAVMNYVKAAELVLEAPDQTAVIRTRKIMDKVSEQILHADSIIRTLRI
                     FTERRDCTKTLESLNNLIEEAIAVCFVGDRRPVIKIRLDLDPALPPVPMDRIQIQQVL
                     VNLIRNSIEAMQAADKRELSLTTGVDEPGFVQVTVRDTGHGLTEEARMSLFQPFITTK
                     ENGMGIGLTICQSIIDAHGGNIWTIVEPSPGATFKFQLPLANSKEFPP"
     gene            3145..3795
                     /locus_tag="VGC27_11315"
     CDS             3145..3795
                     /locus_tag="VGC27_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0283198.1"
                     /translation="MLHTSKAVTGPPIRFLTEDSRGCADTAEVSILVARLLTAANGLD
                     ARIASLEAVPALPTSFNGVCLISAGRDAADSIGALLSSCGLAVWSPYSDGASYEETKY
                     ECALCLVLNMPDQAGLETLELFRRYGIWTPAILIVDNKDEMPADRLLQTGTLAVIPRP
                     VETREFLRWIECICATQLSLRGQWAFRAGMHHKISSDEGRPRNAVGVPCPPALTTT"
     gene            3768..4415
                     /locus_tag="VGC27_11320"
     CDS             3768..4415
                     /locus_tag="VGC27_11320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0283199.1"
                     /translation="MPAGPDHDLNNRSFGNAHVRKQLLVVNGHPDPRSERFCAALCDA
                     YEAGAHAGGWETRRLNVGALAFSDIAARDEVSIEMMDALGNIGWSSQLAIVYPLWLNR
                     PPTGLHTFFDYLWRRHQTADPNVANFERLERVARIIVTMDMPALIYRSLDRRGNGGRE
                     GIQTLSLPGIRSGEPILIGSVNTISSEQRRLWLEAVRLYGEQGALVSLFPYRKSC"
     gene            complement(4489..>5665)
                     /locus_tag="VGC27_11325"
     CDS             complement(4489..>5665)
                     /locus_tag="VGC27_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEY0283200.1"
                     /translation="GRAMVFLRLEDPAMDGEVNYRAILDVLPMPVWFRNRNLTLAWAN
                     RAFVTATNHTTLKDAIEADATLDSSERDMAQEAGKGDGIVSRKLCAVVKGQRRALEIK
                     MSRLSNMNVVGTAIDVTEATQAEAMLQLNADAVADVVDRIETAIVIFGKDKRLVTCNK
                     AYARMWGFTEEWLGAHPSFDDVIDRLRTERRLPDQRDFRAWKRDQLQLFEKADLCGDE
                     TWHLPVGGSVRVRTYPYLPGGIACLFEDITERLSMQVSYNMLAQMQRATLNTVDDGMV
                     VFGPDGRLKLHNDAFVRLWRLSESELEGEPHITKVADLCVSHNGHDGIWNIVLEGVES
                     AEPARYSEWGTVTRSDGRVLSLTLSRIPLGATLAVFADLTDIKRFESALKQKPSSAA"
BASE COUNT         1133 a         1656 c         1582 g         1294 t
ORIGIN      
        1 agattcagga ttatttttcc ttccgcgatc agggattgcg gatgcactct tgagcggcgt
       61 gcgacggcgc agatgcgcgc gaccaactcc gctttgtgat atggcttcgt tatatagtcg
      121 tccgctccgg cgtcgagaga actgaccatc gcccgggtgc tcgcatcgcc ggaaagaacc
      181 agaacgggcg catggacctt ggcggcgcgc aaggccttca gaacattcaa cccgttcata
      241 tccgacaagg agcgatcaag gataatgacg tcgtaatcgc aacgctttcc gagttcgaca
      301 ccttctttgt cgatctcgct gctgttgaaa tcgaaccctt cggaacgcag caccggcttt
      361 atgccggaag cttgcgcggc attgtcctca atcagcaaga ttcgcatttt acaatctccc
      421 aaaggcattc gcccgccgga tgcgaagggg cctggagcgc ttgggcaatc atgtccgggt
      481 ttattgccgt cgcgctccat cgcttggcgg gcaagcgatt tcggggcgat catggcgggt
      541 attgccgcaa ctatatattc ggctttttcc cttaggtgag gctacttagc ggccggccgg
      601 ttcgcggcat gcgatcccgg ttcacgcctc ccgcatacgt atatttactt aggcggcgtc
      661 cttttgcgtc caagacgcgg tgagcgcgat acgcaccaca tcggaaagat tgcgtgcgtt
      721 caatttgtcc atgatgtgcg cccggtgaat ttcgatcgtg cgcggcgata tcccaagctc
      781 gtaagccgcg attttattcg agtgtccggc gacaagctga tcgagaacgc catgttcgcg
      841 cggcgtgagc agggccagca agtgttttgc agttgtcaat tcttcggctt cggcgcgcgc
      901 cttttcaccg gtcgccaacg ctcgttgcac gctttccagc aaccgctcgc acttaaatac
      961 cttttcaagg aattctacgg cgccggcctt catggcgcgc acggcaaggg gcacatcgcc
     1021 gtgacccgtg atgacgatca cgggcaaatc tatgccgcgc ctgacgatct cctcctgcat
     1081 ctcgagcccg ttcatttcag gcatgcaaat gtccacgatc acacagccgc tgtgataaac
     1141 gcggtccgcc agaaagagtt tcgcggatgg atagtctctg acgatatatc cggcgcactc
     1201 cagcagcgtg cgcatggaat cgcgaagaag cgcgttgtcg tccactacgt aaatgaccgg
     1261 ttccgtactc atggtgggaa ctccttactg ttggcaagtg gcaactggaa tttaaaggtg
     1321 gcacccggcg acggctctac aatcgtccaa atgtttccgc cgtgggcgtc aatgatcgat
     1381 tgacagatcg ttaagccaat tcccattcca ttctcctttg tcgtgatgaa gggttgaaag
     1441 agagacattc tcgcctcttc ggttaatccg tgacccgtat cgcgaacggt cacctgtacg
     1501 aacccgggct catcgacgcc cgttgtcagc gacagctcgc gcttgtcggc cgcttgcatg
     1561 gcttcgatgc tgttgcggat cagattgacg agaacttgct gaatttgaat cctatccatc
     1621 ggaaccggcg gcagcgcagg gtcgagatcc agcctgattt tgatgacggg ccgcctgtcg
     1681 ccgacaaaac acaccgcgat tgcttcttcg atcaagttat tgagggattc cagcgttttc
     1741 gtgcagtcgc gccgttccgt gaagatgcgc agagtgcgaa ttatcgagtc cgcatgcaga
     1801 atctgttccg acaccttgtc catgattttc cgcgtgcgaa tgacggcggt ttgatcgggc
     1861 gcctccaaga ccagctccgc ggccttcaca tagttcatga cggcggtgag gggttcggtg
     1921 agtttacgag cgattgcaaa ggacatttgg gccatcgcac tcaacctgga gacatgcagc
     1981 aattcggctt gaagttcgta aagcctcttt tgggcatcgt gccgtccgtt ggtcccgtgg
     2041 acaaagctcg aaaacagaac cttgctccct ccctgtaatt cgacaacggt caattccagc
     2101 ggaaacgtgc tgccgtcgcg atgatggcct acgacaatgc gatatccctc ttgatggtcg
     2161 tgacaggtct gcggcatcag tatcttcacg tcccgtccaa tcgcctcgtc aggcgcatat
     2221 ccgaaaagtt gcgacgccgc gggaccaaac gtctcgatga ggcagttctc gtcgatggtg
     2281 atgatggcgt agggagccgc ttcgaggatc gagcgcagcc taatttcccg tttcttgata
     2341 gcgtcgccgg cgtttctatc gtcggtcagt tcgcgcagga tcgtgacgaa aattcgccgg
     2401 tcgtgcagtt cgccttcgcc aaccgaaaat tgtaccggga agatcgagtt gtctttgcgc
     2461 cgcccgacgg tttcgccact cttcgcgatt cccatcgcgc tgatcttaga gtcgaaaaac
     2521 gggcaatcgc cgcgctcgcc gtaatgcggc atcagcatct tcgcgtgccg gccgacaact
     2581 tcgtttttcg ggtatccgaa tatttcttcg caagcaccgc tgtagatctg caccatccct
     2641 tgtgcgtttg ttacgaggat gccgtccgca gccgaggaga taagagcact gagaagcgcg
     2701 tcattatcag gggcgaccgt gtttcgaatt tcgcttcttt ttgccgctct cgctttttcg
     2761 atgattaagt gtccttaagg ttcaaatact actacaggcg cgaggtttgt cataaaggag
     2821 caactcgcag ttgatccatg ccccactaga taaagtgtct gcggaagtac ggatgtctat
     2881 tatgtgccga tttagttacc gtctgccgac ttgttgcatt cgcgcatatc gataccgcga
     2941 agcttgacgc gttgcgcctt caaatttttg ccgcggtcca tgttttccgc ccgcaaaggg
     3001 cagaccaagc aattcccatc ccgttgcttc cgtcgccacc cacgcaattc tacctaggaa
     3061 gaatacctag ctatcaactc caaaggttgt gccgcaaatt aaccaagaga gagaaggtgc
     3121 gaaccgaatg caatggagca gaacatgctg catacgagca aagccgtaac cgggccgccg
     3181 atacgcttct tgaccgagga ttcccgcgga tgcgccgaca ccgccgaagt ttctattctt
     3241 gtggcgagat tgctgaccgc agcgaacgga cttgatgccc gtattgcgag ccttgaggcc
     3301 gtgcctgccc tgcctacctc tttcaacggg gtctgtctga tctcggccgg gcgggatgcc
     3361 gccgattcca tcggcgcact tctttctagc tgcggtcttg ccgtctggtc gccatattcc
     3421 gacggcgcgt cttatgaaga gacaaaatac gaatgcgcgc tctgcctcgt cttgaacatg
     3481 ccggaccagg ccggtttgga gacgctggag ctgttccgca gatacggcat ctggacgccg
     3541 gcgatcctca ttgtcgacaa caaggacgag atgccggcgg acagattgct gcagacaggc
     3601 acgcttgccg tcattcccag accggtcgaa acgcgcgagt ttcttcgctg gatcgagtgc
     3661 atttgcgcga cccaattgtc cttgcgcggc cagtgggcgt ttcgtgcagg catgcaccat
     3721 aaaatatcgt cagacgaagg acgtcctcgg aacgccgttg gcgttccatg cccgccggcc
     3781 ctgaccacga cctgaataac cgctcttttg ggaacgcgca cgtgagaaaa cagctcctcg
     3841 tcgtcaatgg tcacccagac ccaagatcgg aacgattttg cgccgcgctt tgcgatgcct
     3901 acgaagcagg tgcgcacgcc ggcggatggg aaacccggcg tcttaacgtc ggcgcgctgg
     3961 ccttttccga tatcgcggct cgcgacgaag tgtcgatcga gatgatggac gcattgggca
     4021 atatcggctg gtcgagccaa cttgccatcg tctatccgct ttggctcaac cggccgccga
     4081 cgggcttgca tacgtttttc gattatttgt ggcgccggca tcagaccgcc gatcccaatg
     4141 tcgcgaactt cgaacgtttg gaacgcgtag ccagaattat cgtgacgatg gatatgcccg
     4201 cgctcattta ccgctcgctg gaccggcggg gcaacggcgg ccgggaagga attcagacgc
     4261 tatcgcttcc gggcatccgc tcgggcgaac cgatcctgat cggcagcgtc aacaccatat
     4321 cgagcgagca gcgcaggctt tggttggagg ccgtgcgctt gtatggagag caaggggcgc
     4381 tggtatcgct gttcccttat cgcaaatcgt gttgaagcgg agtttggttt cgaagcgacc
     4441 ggctattgag cgatccgccg agcaatatat gttcatcgcg atcttgcttc acgccgcgct
     4501 cgacggcttt tgcttgagag cggattcgaa gcgtttgatg tccgtcaggt ccgcgaatac
     4561 cgcgagcgtc gcgccgagcg gaatccgcga taacgtcaac gacaagacgc ggccatccga
     4621 gcgggtcacc gtaccccatt cgctatagcg cgccggctcg gcggattcca cgccttccag
     4681 cacaatgttc cagatgccgt catggccgtt atgcgagacg cagagatcgg ccacttttgt
     4741 gatatgcggt tctccctcca attcgctttc gctcaaccgc cacaggcgaa cgaacgcatc
     4801 gttgtgcagt ttcagccgcc cgtcgggtcc gaatacgacc atgccgtcgt caacggtgtt
     4861 gagcgtggct ctctgcatct gggccagcat gttatacgaa acctgcatac tgagacgttc
     4921 ggtgatgtcc tcgaacaggc aagcaatgcc gccgggaaga taggggtacg tcctcacgcg
     4981 cacgcttccg ccgaccggca gatgccaagt ctcgtcgccg cacaagtcgg ctttctcgaa
     5041 caactggagt tgatcgcgtt tccacgccct gaaatcgcgc tggtccggca ggcgccgttc
     5101 cgtacgcaga cgatcgatga cgtcgtcaaa ggacggatga gcgcccaacc attcttccgt
     5161 gaatccccac atccgcgcat aggccttgtt gcaggtgacc agccgtttat cctttccaaa
     5221 aatgacgatt gccgtttcga tccggtccac cacgtcggca acggcatccg cgttgagttg
     5281 caacatggcc tcggcctgcg tggcttccgt cacatcaatc gcggtaccga ccacgttcat
     5341 attcgaaagc cgcgacatct tgatctcgag cgcccggcgt tggcctttga cgacggcgca
     5401 gagtttgcgg ctgacgatgc cgtcgccttt gccggcttcc tgcgccatgt cgcgctcgga
     5461 gctatccaga gtcgcatccg cttcgatcgc gtccttcagc gtcgtgtggt tcgttgccgt
     5521 cacaaaggcg cgattggccc acgccaaggt gaggtttcgg ttgcgaaacc agaccggcat
     5581 cggaaggacg tccaagatgg cgcgataatt cacttcgcca tccatggcgg gatcttcgag
     5641 ccgcagaaac accattgcac ggccg
//
LOCUS       DASWPU010000536         3487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_84454,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000536 DASWPU010000000
VERSION     DASWPU010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84454"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VGC27_11330"
     CDS             <1..688
                     /locus_tag="VGC27_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283201.1"
                     /translation="APMAIVAANGGTDFIYVPEDANRRATAKRIFDKLLEAPYVGALF
                     VNDDLLKNADPKDFAGALPMSAVNLIGSSKVPQPAIVIGFRSFVVDGCELGEQLCTAE
                     IADTTLHTGQGMHGSFSRADTRNFMAAIGPDFKAKFVDTAPVGNVDIAPTAAHILCVK
                     LSGPGTLKGRVITEALKGGKMPKVVSQTIASEPAANGLRTIINIQEVDSTKYFAAGGI
                     PGRTVGLSVR"
     gene            803..1990
                     /gene="rnd"
                     /locus_tag="VGC27_11335"
     CDS             803..1990
                     /gene="rnd"
                     /locus_tag="VGC27_11335"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEY0283202.1"
                     /translation="MRIVETTADLKTLVGELAGAPYLALDTEFMRDSTYWPILCLLQI
                     AAPGVAAIVDPLAEGIDLSPFFTLLKDPRIVKVLHAGRQDIEIFWHKGHVIPDPLFDT
                     QIVAMVCGFGEAASYETLTRKIAHVQVDKSARFTDWARRPLAKRQLEYALGDVTHLCT
                     IYEALSAQLAKNKRESWVAEEIAALKSEELYALDPAQAWKRLKPRTGNKRFLTALAAV
                     AAWREREAQTRDVPRNRILKDEVLLEIASQPPEGAEALERIRAVPKGFAASRMGKSLL
                     EAISESREALPPEVAEQPERRRRKREPSPAAIDLLRTLLRLKAEEAGVAPRLIANSDD
                     IERLAAGDDDGVAALSGWRGEVYGKDAVALRKGTLAIALENGEAVVVELEDAEGPSSE
                     TAK"
     gene            complement(2035..2493)
                     /locus_tag="VGC27_11340"
     CDS             complement(2035..2493)
                     /locus_tag="VGC27_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283203.1"
                     /translation="MSENALCRGNKGCVEAAPLGEEQTMKIALKGFVCAIGLIGVVGQ
                     TAYAEPPAAPPPAQRQVCINPGSIEHLAFPDDSTILFHMRTGPVRIWKNELKRACPGL
                     KFEGGVAWEIRGGEICGNMQTFYVLRRWTPCMLGSFTPYTPPAPPASPAQ"
     gene            complement(2530..3288)
                     /gene="scpB"
                     /locus_tag="VGC27_11345"
     CDS             complement(2530..3288)
                     /gene="scpB"
                     /locus_tag="VGC27_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY0283204.1"
                     /translation="MNRIVHMKETLDAAVADDVLISPAEGSNEPVSGVNIEHLRMAEA
                     LLFAASEPLSIDALASSLPEGADVGSLVAELQRMYEGRGVTLVCVAEKWQFRTAADLS
                     FLLRREKPEQRRLSRAALETLAIIAYHQPVTRAEIEDIRGVMLSKGTVDTLMEIGWVK
                     IRGRKRTPGRPVTYGTTEAFLVQFGLESVGHLPGLDELKAAGFLEAIPPSGFDVPLPS
                     DQLGPDEDPFEGEETGEELATGGEDPFAPVSSGE"
     gene            complement(3285..>3487)
                     /locus_tag="VGC27_11350"
     CDS             complement(3285..>3487)
                     /locus_tag="VGC27_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283205.1"
                     /translation="LGRIPDWSGLASLLPFEWSGGQRRRSALASTLLACLELARDGRI
                     ELRQLAPFDEVFVRDRVSEAAK"
BASE COUNT          657 a         1154 c         1072 g          604 t
ORIGIN      
        1 ggcgccgatg gcgatcgtcg cggcgaacgg cggcacggat ttcatctatg tgcctgaaga
       61 cgcaaaccgc cgcgccaccg ccaagcgcat cttcgacaag ctgctggagg cgccctacgt
      121 cggcgcgctg ttcgtcaacg acgacctgct caagaacgcg gaccctaagg acttcgccgg
      181 cgcgctgccg atgagcgccg tgaacctgat cggctcctcg aaagtgccgc agcctgccat
      241 cgtgatcggc ttccggtcct tcgtggtgga cggctgcgag ctcggcgaac aactttgcac
      301 ggcggaaatc gccgacacga ccctgcacac cggccagggc atgcatggca gcttcagccg
      361 cgccgacacg cgcaacttca tggccgccat cggaccggat ttcaaagcca agttcgtcga
      421 cacggcgccg gtcggcaatg tcgacattgc gccgacggcg gcgcacattc tgtgcgtgaa
      481 attatcgggg ccgggtacgc ttaagggccg ggtgatcacc gaagcgctca agggcggcaa
      541 gatgcccaag gtcgtcagcc agacgatcgc ctctgaaccc gccgccaatg gcttacgcac
      601 catcatcaac atacaagagg ttgattccac aaaatatttt gccgcaggag gcattcccgg
      661 acggaccgtc gggctttccg tgcgctgacc ggcccggggc tgaacgatcc tgctgcgaag
      721 cgccttgcgg cgcatcgccc ctggcggcag gaaacgggaa ttaaacaagg cacgtccggc
      781 ccgaatccgc tatcaccatg gcatgcggat cgtggaaacc acggcggatt tgaagacgtt
      841 ggtgggcgag cttgcgggtg cgccttacct tgccctcgat accgagttca tgcgggattc
      901 gacctattgg ccgatccttt gcctgttgca gattgccgct cccggcgtcg cggccattgt
      961 cgatccgctg gccgaaggca tcgatctgtc cccgttcttc acgctcctca aagacccgcg
     1021 catcgtcaag gtgctgcatg ccgggcggca ggacatcgag attttctggc ataaggggca
     1081 cgtcatcccc gatcccctgt tcgacaccca gatcgtggcg atggtctgcg gcttcggcga
     1141 agcggcgtcg tacgagaccc tgacgcgcaa gatcgcccac gtccaggtgg ataagtccgc
     1201 gcgcttcacc gattgggcgc gccgtccgct cgccaaacgt cagctcgaat atgcgctggg
     1261 cgacgtgacc catctgtgta ccatctacga agcgctctcg gcccagctcg ccaagaacaa
     1321 gcgcgagagc tgggtggcgg aagagatcgc cgcgctcaaa agcgaagaat tgtacgcgct
     1381 cgatccggcc caggcctgga agcgcctgaa gccgcgcacc ggcaacaagc ggttcctgac
     1441 cgcgctggcg gcggtggccg cctggcgcga gcgcgaggcg cagacccgcg atgtcccgcg
     1501 caaccgcatt ctcaaggatg aagtgctgct ggagatcgcc tcccagcccc ccgaaggcgc
     1561 cgaggcgctc gaacgcatcc gcgccgtgcc gaagggcttt gccgcctcgc gcatgggcaa
     1621 gagcctgctg gaggcaatat cggaaagccg cgaggcgctg ccgcccgaag tcgccgaaca
     1681 gcccgaacgc agacggcgca agcgcgagcc ttcgcccgct gcaatcgatc tgctcagaac
     1741 cctgctgaga ctcaaggccg aagaagccgg ggtcgcgccg cgcttgatcg ccaacagcga
     1801 cgacatcgaa aggctggcgg ccggcgacga cgacggcgtc gccgcgctgt ccggctggcg
     1861 gggcgaggtg tacggcaagg acgccgtcgc tttgcgcaag ggcacgctcg ccatcgcgct
     1921 cgaaaacggc gaggccgtgg tggtggagct ggaagacgcc gaagggccga gtagcgaaac
     1981 agcgaaatag cgaatagagg ttttttctat tcgctattcg ctactcccgt tcgcctattg
     2041 cgccggcgaa gccggaggcg ccggcggcgt gtagggcgtg aaagagccaa gcatgcacgg
     2101 cgtccagcgg cgcaggacat agaatgtctg catatttccg caaatctcgc cgccgcgaat
     2161 ctcccaggcg acaccaccct cgaatttaag tccgggacag gcccgcttca gctcgttctt
     2221 ccaaatccgc acgggcccgg ttctcatatg aaacaaaatg gtgctgtcgt cgggaaatgc
     2281 caggtgctcg atactgcccg ggttgatgca cacttgtctc tgtgccggcg gcggtgctgc
     2341 cgggggttcg gcataggccg tctggccgac cacgccaatc aaaccgatag cgcaaacaaa
     2401 acccttcaga gcgatcttca tcgtctgctc ctcacctaat ggggctgctt ccacgcagcc
     2461 cttattgccc ctgcacagag cattctcgct catttcgggc tgccgctcaa tcgcacttca
     2521 ggttgcgccc tattcgccgg atgaaaccgg cgcgaagggg tcttcgcccc ccgtggccag
     2581 ctcttcaccg gtctcctcgc cttcgaacgg atcttcgtcc gggccgagct ggtcgctggg
     2641 caacggcacg tcgaagccgg acggcggtat ggcttccagg aagccggcgg ctttcaattc
     2701 gtcgaggccg ggaagatggc cgacactctc cagcccgaac tgaaccagga acgcttccgt
     2761 ggtgccgtag gtcaccggac ggcccggcgt gcgcttgcgc cccctgattt tgacccagcc
     2821 gatttccatc agcgtgtcca cggtgccctt cgacagcatg acgccgcgga tgtcctcgat
     2881 ttcggcccgg gtgacgggct ggtggtaggc gatgatggcc agggtctcga gcgccgcgcg
     2941 cgacagccgc ctctgttccg gcttctcccg gcgcaacagg aatgacaaat ccgccgccgt
     3001 acggaattgc catttttccg cgacacagac gagggttacg ccacgtccct catacatgcg
     3061 ttgaagttcg gcgacgaggc tgccgacatc cgccccttcc ggcagcgacg acgccagggc
     3121 atcgatgctc aacggttcgc tggctgcgaa cagcaaggcc tcggccatgc gtaaatgttc
     3181 gatgttgacg ccggacacag gctcattact cccttccgcc ggcgaaataa gcacgtcgtc
     3241 ggcgaccgct gcgtccagcg tttctttcat atgcacgata cggttcattt cgcggcctcg
     3301 ctgacgcggt cccttacaaa aacttcgtcg aacggcgcaa gctgacgaag ctcgatccgt
     3361 ccgtccctgg ccagttctag gcatgccaga agcgtgctcg caagcgccga acgccgccgc
     3421 tgcccccccg accactcgaa cggcagcaga ctggccaagc cgctccagtc ggggatgcgg
     3481 ccaagca
//
LOCUS       DASWPU010000537         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_72152,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000537 DASWPU010000000
VERSION     DASWPU010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72152"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VGC27_11355"
     CDS             <1..347
                     /locus_tag="VGC27_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0283206.1"
                     /translation="RFGAAGFLAAYNAGPKRLEDYLAGLRPLRDETERYLAILTQMLP
                     DLQIGVAQPAVRVPTDWRVSGLFIASPTSHSPLNITPNILASQNTTTSQTFALGPQSG
                     GLFVPVRLLDQR"
     gene            597..>1712
                     /locus_tag="VGC27_11360"
     CDS             597..>1712
                     /locus_tag="VGC27_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HEY0283207.1"
                     /translation="MPRRDDDFRVRPGRIRDGGTCKIQSYVGEVTRAAKKAGHTGKRF
                     GGGASPKGGSRFGRGRNIAARIGLNASARRVVVMARVVRHHSRKFRAASLALHINYLS
                     REGVTRDGSDARMFNAESDNADGKAFVERCQDDRHHFRFTVSPEDAGQMADLKAFTRE
                     LMKTAERDLETRLDWIAVDHWNTDNPHIHVLVRGKADDGKDLVISRDYISRGLRLRAS
                     ERVTLELGPRSEHEIRTALAKDVVAERWTGLDRALRDMADDGIGIVDLRPTGAKDDPE
                     IRRLLVGRAQTLERLGLADYVAPGRWTIKPRLEETLRGLSIRGDIIKTMHRAMAESGR
                     ELDISGYALHDGPPETPVAGRLTARGLHDELSGTAYAV"
BASE COUNT          328 a          537 c          550 g          297 t
ORIGIN      
        1 accgcttcgg ggcggcgggg tttctcgcgg cttacaatgc aggaccgaag cggcttgagg
       61 actatctggc tggccttcgg ccgctccgcg acgagaccga gcgttacctt gcgatattga
      121 cgcagatgtt gccggatttg cagattggtg tcgcgcaacc tgctgtgcgg gttccaactg
      181 actggcgagt ttcgggcctg ttcatagcgt cgccaacatc gcattcgccg ttgaacatta
      241 cgcctaacat tcttgcatct cagaacacga cgacatcaca gacttttgcg ctcgggccgc
      301 agtcgggcgg actgttcgtg ccggtccgac tgctcgacca gcggtgagcg ccgtgctctt
      361 tgccatcacc cccaaccacg actccgaaat cggtgtcatc atcaggtcca accaccacta
      421 ccagccaacc aggagagcgc aagataaaag ggccgtccgc gttcggccac atgtggttgg
      481 cgcgcggaag aaatttcggg gacaggcgag aacactcgtc cgcggtttgc tgaatttctt
      541 caggtgttct gcgtctttcg cggtccgcac cccgtcgttt ctgcgcgagg ctacgcatgc
      601 cacgacgtga cgacgacttt cgcgtccggc cgggacgcat ccgcgatggc ggcacgtgca
      661 agatacagag ctatgtcggt gaggtcacgc gggccgccaa gaaggccggc cacaccggca
      721 agcgtttcgg cggcggagcg tcgcccaagg gcggctcacg cttcggccgc gggcggaaca
      781 tcgctgcgcg gatcgggctc aatgcatcag cccggcgcgt cgtggtgatg gcgcgggtag
      841 tgcgccatca cagccggaag tttcgggcgg cgtcattggc actgcacatc aactatctca
      901 gccgcgaagg cgtcacccgc gacggcagtg acgcccggat gttcaacgcg gaaagcgaca
      961 atgccgacgg caaggctttt gtagagcggt gtcaggacga ccggcatcac ttccgtttca
     1021 cagtgtcgcc ggaggacgcc ggccaaatgg cggacctcaa ggcgttcacc cgcgagctga
     1081 tgaagacggc cgaacgcgac ctggagaccc gccttgattg gatcgcggtc gatcactgga
     1141 acaccgacaa cccccacatc catgtcctgg tacgcggcaa agccgacgac ggcaaggacc
     1201 tcgtcatcag ccgcgattat atcagccgag gactgcgcct gcgcgcctca gaacgggtta
     1261 cccttgagct ggggcctcgc agcgagcacg agattcgcac ggcgctggcg aaggacgttg
     1321 tggcggagcg ctggaccggg ctcgaccggg cgctacgcga tatggccgat gacgggatcg
     1381 gcatcgtcga tttgcgtcca acaggcgcca aagacgaccc ggagattcgg cggctactgg
     1441 tcggacgagc gcagaccctg gaacgcttgg ggctagccga ctacgttgcg ccagggcgtt
     1501 ggaccatcaa accacggctg gaggagacgc tgcggggcct ctcaatccgc ggtgacatca
     1561 tcaaaacgat gcaccgcgcc atggcggaat ctggccggga actggatatc agcgggtacg
     1621 ccctccacga tgggccgccg gaaacacccg tcgccggacg gctgacagcg cgcggtctcc
     1681 atgacgagtt gtccggtacc gcctatgccg tc
//
LOCUS       DASWPU010000538         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_47609,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000538 DASWPU010000000
VERSION     DASWPU010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47609"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /gene="trbB"
                     /locus_tag="VGC27_11365"
     CDS             complement(<1..876)
                     /gene="trbB"
                     /locus_tag="VGC27_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HEY0283208.1"
                     /translation="MLRTALGPAIAGFLEDASVVEVMLNPDGHVWVDRLSGGLEDTGH
                     RISPNDSERIVRLVAHHVAIEVHAGAPRVSAELPETGERFEGLLPPVVTAPCFAIRKP
                     AVAVFTLDDYVEAGIMTAAQAGLLRTAVLARKNVLVAGGTSTGKTTLVNALLAEIAKT
                     SDRIVLIEDTRELQCTARNLVALRTKDGAASLSDLVRSSLRLRPDRIPIGEVRGAEAL
                     DLLKAWGTGHPGGVGTLHAGSAIGALRRLEHLIQEAVVTVPRALIAETIDIVAVLAGR
                     GAARHLAELAEVDGLG"
     gene            complement(1151..1582)
                     /locus_tag="VGC27_11370"
     CDS             complement(1151..1582)
                     /locus_tag="VGC27_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY0283209.1"
                     /translation="MQRERYSVRLPANLLKQLDELALRKRVGRAAIVETALASYLSPD
                     GADQLETALARRLDRINRQLDRMERHIAITNETLALFIRTWLIQTPPLPDAAQAAAQA
                     KGRQRYEAFVEAVGRRLARGKSLIDEITREAAADPNKQTLE"
     gene            complement(1583..>3293)
                     /locus_tag="VGC27_11375"
     CDS             complement(1583..>3293)
                     /locus_tag="VGC27_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HEY0283210.1"
                     /translation="GGIAAIAVAIAMSVWRAREAKRTVTYGSARWADKHEVRTAGLLH
                     PDGVMIGRLKNTYLRHNGPEHVLCFAPTRSGKGVGLVVPTLLTWSGSVIVHDIKGENW
                     TLTSGWRQRFGRVLLFDPTNPDSAAYNPLLEVRRGEREVRDVQNIADILVDPEGALER
                     RNHWEKTSHSLLVGTILHVLYAEPDKTLAGVANFLSDPKRSIEKTLAAMMTTPHLGEG
                     QVHPVVASAARELLNKSENERSGVLSTAMSFLGLYRDPVVAQVTRRCDWHIADLVEGP
                     PPVTLYLVVPPSDIARTKPLVRLLLNQIGRRLTEQLTPDRSHHRLLLMLDEFPALGRL
                     DFFESVLAFMAGYGLKAFLIAQSLNQIEKAYGLNNSILDNCHVRVSFATNDERTAKRI
                     SDALGIATELRAMKNYAGHRLSPWLGHLMVSRQETARPLLTAGEIMQLPVDDEIVLVS
                     GCPSIRAKKVRYYQDRQLQHRILPPPRPELQETLGCSEPNSEKNGAWKDAIAPQADDD
                     TNGGIRREPELPEHEEVVPKPPEPQREFDLAEDDEAPPLPLFDPRYIARQTALDRDDD
                     MGM"
BASE COUNT          608 a          965 c         1093 g          627 t
ORIGIN      
        1 accgaggccg tcgacttcag cgagttcggc caaatgccgc gcggcgccgc ggccggcgag
       61 gacggcgacg atgtcgatag tttcggcgat gagggcgcgt ggcaccgtga ccacggcttc
      121 ctggatcaga tgctccaacc ggcgtagcgc tccgatcgcc gatcccgcat ggagcgtgcc
      181 aacgccgccg ggatggccgg tgccccaggc tttcaagagg tccaaggctt cggccccgcg
      241 tacctccccg atgggaatgc ggtcggggcg caaccgaagc gacgaacgga cgaggtcaga
      301 caatgatgct gcaccatctt tggtgcgcaa cgccacgaga ttgcgcgcgg tgcattgcag
      361 ctcgcgcgtg tcctcgatga gcacgatgcg atcggaggtc ttggcgatct cggccagcag
      421 cgcgttgacg agggtggtct taccggtgga ggtgccgccg gcaaccagca cgttcttgcg
      481 cgccaaaacg gcggtccgca gaagtccggc ctgcgcagcc gtcatgatgc cggcctcgac
      541 gtaatcgtcg agcgtgaaca ccgcgacggc gggcttgcgg atggcaaagc aaggggcggt
      601 taccaccggc ggcaagagtc cctcgaaccg ctcacctgtc tcgggcaact ccgcggaaac
      661 acgtggggct ccggcatgaa cttctatggc gacgtggtgc gccaccagac gcacaatgcg
      721 ttccgagtca ttgggagata tgcgatgtcc ggtatcttca aggccaccgg agaggcgatc
      781 gacccagaca tgtccgtcgg ggttgagcat gacttcgacg acggacgcgt cctcaagaaa
      841 acctgcgata gccggaccaa gtgccgtacg caacatgcgt gagctgcgtg cgaaggcttc
      901 ggatcggaaa gtggaaacgc tcacgcgatt gaccccaggt attggcgcgc cagccgcgcc
      961 gttgatgggg tcgaatagaa agagcgctaa tctaccaaac gcaacaagag agatgcaggc
     1021 gtcgcaggct ggcgtacaaa gacaggcact cggcggtttg tttgggaaca ggcgcacgaa
     1081 atcggcagcg catggctgtt gcttcttggt gagttgcatc tttgaggagg gtgtcgagga
     1141 agcagggccg ctattccagc gtctgcttgt tggggtcggc tgctgcctcc cgcgtgatct
     1201 catcgatcag ggatttgccg cgggcaagcc tgcggcccac agcctcgacg aaggcttcat
     1261 atcgctgacg gcctttggcc tgtgcggcag cctgcgctgc gtctggcagg ggcggcgttt
     1321 ggatcagcca tgtgcggatg aacagcgcca gcgtctcgtt ggtgatggcg atgtggcgtt
     1381 ccattcggtc aagttggcgg ttgatgcggt ccagccggcg ggcgagtgcc gtttccaact
     1441 gatcggctcc gtctggagac aggtagctcg caagcgcggt ctcgacgatg gccgcacgcc
     1501 cgacccgctt gcggagggca agttcgtcca actgtttgag caggttcgcc ggtagtcgca
     1561 ccgaatagcg ttcgcgctgc atctacatcc ccatgtcatc gtcacggtcg agagctgtct
     1621 gccgcgcgat ataccgagga tcgaacaagg gcagtggcgg cgcctcgtcg tcctccgcga
     1681 ggtcgaactc gcgctggggt tcgggcggtt tcggtacgac ctcttcgtgt tccggcaatt
     1741 ccggctcacg ccgtatcccg ccattggtat catcgtctgc ctgtggagcg atcgcgtcct
     1801 tccaggctcc attcttctcg gaattgggtt ccgagcatcc gagtgtctct tggagttcag
     1861 ggcgcggcgg tgggaggatg cgatgctgca gttgccggtc ctggtaatag cgcaccttct
     1921 tggcgcggat cgatgggcac ccggagacga gcacgatctc atcatcgacc ggcagctgca
     1981 tgatctcgcc ggccgtgagc agcggccgcg ccgtctcctg ccgcgaaacc atcagatgtc
     2041 ccagccatgg gcttaagcga tggccggcat agttcttcat ggcacgcagt tcggtggcga
     2101 tgcccagcgc atcggaaatg cgtttggcgg tgcgctcgtc gttggtggca aagctcaccc
     2161 gcacatggca gttgtcgagg atggaattgt tgaggccata ggccttctcg atctgattga
     2221 gggactgtgc gatcagaaaa gccttcaatc catagcccgc catgaaggcg aggacggatt
     2281 cgaagaagtc gagccggccg agggccggga attcgtccag catcagaagc aggcgatggt
     2341 gtgaccggtc cggcgtcaat tgctcagtca gtcttcgtcc gatctgattg agcagaagcc
     2401 gcaccagagg cttggtgcgt gcgatgtcgg aaggcggcac gaccagatag agcgtcaccg
     2461 gaggggggcc ttccaccaga tcggcgatat gccagtcaca ccggcgtgtg acctgcgcca
     2521 ccacagggtc gcgataaagg cccagaaagc tcatggcggt cgagagcacg ccggagcgtt
     2581 cgttctcgct cttgttgagg agttcgcggg cggcactggc gaccaccgga tgaacttgtc
     2641 cttcgcccag atggggtgtg gtcatcatgg cggccagcgt tttctcgatt gagcgtttgg
     2701 gatcggaaag aaaattggca accccggcca gtgtcttgtc cggttcggcg tacagtacat
     2761 ggaggatcgt tccgaccagc agggaatggc tcgttttctc ccaatgattg cgccgctcca
     2821 aagcgccttc cggatccacc aggatatcgg cgatgttctg cacgtcgcgc acttcgcgtt
     2881 cgccgcggcg cacttccaat aggggattat aggcagcgct gtccgggttg gtgggatcaa
     2941 agaggagcac ccggccaaag cgttggcgcc agcctgacgt gagggtccag ttctcgccct
     3001 tgatatcatg cacgatcaca gaaccggacc aggtcagaag tgtggggacg accaacccca
     3061 cgcccttgcc gctccgggtc ggggcaaagc acaacacatg ctcaggaccg ttgtggcgga
     3121 gataagtgtt cttcagccgt cctatcatca cgccatcggg gtggagcaga ccggcggtgc
     3181 ggacttcatg cttgtccgcc cagcgggccg aaccataggt gacggtgcgc ttggcctcgc
     3241 gggctcgcca caccgacatg gcgatggcga ctgcgatcgc ggcgatgccg ccg
//
LOCUS       DASWPU010000539         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_4346, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000539 DASWPU010000000
VERSION     DASWPU010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4346"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1879
                     /locus_tag="VGC27_11380"
     CDS             221..1879
                     /locus_tag="VGC27_11380"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY0283211.1"
                     /translation="MGGRRESMDYDVVIVGGGPAGLAAAIRLKQLAAEAGRGVGVCVL
                     EKGSEIGAHILSGAILDPVALNELIPDWKEGDAPLDTQVTDDRFYFLTRNASIPLPHF
                     AMPPLMSNHGMYIVSLGNLCRWLAAEAEALGVEIYPGFPAADLIFGEKGEVKGVVTGD
                     FGVARDGHRKETYAPGMELRGKYTLFAEGARGSLTKQLIARYALEEGREPQKFGIGIK
                     ELWELAPEKHKRGLALHTMGWPLANETGGGLFMYHWGEHFCSIGFVLHLNYKNPYISL
                     FDEFQRVKHHPRLRPFLEGGRRIGYGARAVTEGGLQSVPRLAFPGGALAGCGAGFMNV
                     PRIKGSHNAMKSGMLAAEAVFAALGAGRAGDTLESYETAYRHSDVYRDLKRVRNVKPL
                     WSKLGTFPGIPLGALDMWTNQLFGFSFFGTLRHGGPDHMTLRKAGVSNPVAYPKPDGV
                     ISFDKNSSVFLSNTNHEEDQPVHLKLADPALPLRVNLPEYAEPAQRYCPAGVYEIVGE
                     AGEARFQISAQNCVHCKTCDIKDPSQNITWVTPEGGGGPNYSNM"
     gene            1894..>2372
                     /locus_tag="VGC27_11385"
     CDS             1894..>2372
                     /locus_tag="VGC27_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0283212.1"
                     /translation="MRILPLIAALGLSAAIAGCAGARVTVASVSPILDRAPVTEKSTS
                     VYGDYLSARFAADHHDLAEAAKYFRASLDGDPKDPQLLTFAFFYAASAGQIDQAAELA
                     GRLASVSPGDRAARLTLAVAALKKRDYKAARTQIAKSAKGPFTLFTVALIDAWAAAG"
BASE COUNT          424 a          730 c          781 g          437 t
ORIGIN      
        1 agagtcggga actttcttcg caaaccggtc caccggctct tcgccgacag cttcgtccgc
       61 tccggcttcg aggagccaag cgagagcggc gagaggattc gtttcggtcg ggtcggccat
      121 gaccgcagca tagggtagag aagtgccgcg cgcgacggct tcggttgacc gggccgctcg
      181 ccccgccgca taagctgtgg gggttatctc aggtctgggc atgggcggcc ggcgcgaaag
      241 catggactac gacgtggtga tcgtcggcgg cgggccggcg ggccttgccg ccgcgatccg
      301 gctgaaacag cttgcggccg aggccggacg cggcgtcggc gtctgcgttc tggagaaggg
      361 gtcggagatc ggggcgcata ttctttcggg cgcgatcctc gatccggtgg cgctgaacga
      421 attgatcccg gactggaagg aaggggatgc gccgctcgac acgcaagtga ctgacgaccg
      481 cttttatttc ctgacccgaa acgcctccat ccctttgcct catttcgcca tgccgccgct
      541 gatgagcaac cacggcatgt atatcgtaag cctgggcaat ctctgccgct ggctggcggc
      601 cgaggctgag gcgctcggcg tggaaatcta tccgggcttt ccggcggccg acctcatctt
      661 tggagagaag ggcgaggtga agggcgtcgt caccggcgat ttcggcgtgg cgcgcgacgg
      721 ccatcgcaag gaaacctacg cacccggcat ggagcttcgc ggcaaataca cgctgttcgc
      781 ggaaggcgcg cgcggctcgc tgaccaagca gctcatcgcc cgatacgcgc tggaggaagg
      841 ccgcgagccg cagaagttcg gcatcggcat caaggaacta tgggaactgg cgccggaaaa
      901 gcacaagcgc gggctggcgc tgcatacgat gggctggccg ctggccaatg aaaccggcgg
      961 tgggctgttc atgtatcatt ggggcgagca cttctgttcc atcggcttcg tgctgcatct
     1021 gaattataaa aacccgtata tttccctctt cgacgagttt cagcgggtga agcaccatcc
     1081 gcgtctccgc cccttcctcg aaggcggcag gcgtatcggc tacggcgccc gcgcggtgac
     1141 ggaaggcggc cttcagtcgg tgcccaggct ggctttcccc ggcggcgcgc tcgccggttg
     1201 cggcgccggt ttcatgaacg tcccgcggat caagggcagc cacaacgcca tgaaatcggg
     1261 catgctggcg gcagaagccg tttttgcggc gctgggagca ggccgtgccg gcgacacgct
     1321 ggagagctat gagaccgctt accgccacag cgatgtttac agggacctga agcgggtaag
     1381 gaatgtaaag cctctctggt ccaagctggg gacctttccc ggcatcccgc tgggggctct
     1441 cgacatgtgg accaaccaac tgttcgggtt cagctttttc ggtacgctcc ggcacggcgg
     1501 gccggaccat atgacgctgc ggaaggccgg ggtaagcaac cctgtcgcct atcccaagcc
     1561 ggacggcgtc atcagcttcg acaagaattc cagcgtgttc ctctcgaata ctaaccatga
     1621 agaggaccag ccggtgcatt tgaaacttgc ggatccggca cttcctctgc gggtcaatct
     1681 gcccgaatat gccgaaccgg cgcagcgcta ttgcccggcg ggcgtatatg agattgtggg
     1741 cgaggctggc gaggcccgtt tccagatcag cgcccagaat tgcgtgcatt gcaaaacctg
     1801 cgacatcaag gatccgtcgc agaacattac ctgggtgacg ccggaaggcg gaggcggtcc
     1861 gaactattcg aacatgtaag gagtataaga taggtgcgta ttctaccgct catcgccgcg
     1921 ttgggtctgt ccgcggctat cgccggctgt gccggcgcca gggtcacggt tgcgtcggtc
     1981 tcgccgatcc tcgacagggc gccggttacg gagaagtcca ccagcgttta cggcgactat
     2041 ctttccgccc gctttgcggc ggaccatcac gacttggccg aagcggcgaa gtacttccgt
     2101 gccagcctgg atggcgaccc taaggatccg cagcttctga ccttcgcgtt cttctatgcc
     2161 gccagcgccg gccagatcga ccaggcagcc gagcttgccg gccggcttgc ttccgtgtcg
     2221 ccgggggatc gcgccgcgcg cctgacgctc gccgtcgcgg cgctgaagaa acgcgactac
     2281 aaggcagcgc ggacccagat cgccaaatcc gccaaagggc cgttcaccct gttcacggtc
     2341 gcgctgatcg acgcctgggc cgctgccggg at
//
LOCUS       DASWPU010000540         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_90236,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000540 DASWPU010000000
VERSION     DASWPU010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90236"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VGC27_11390"
     CDS             <1..898
                     /locus_tag="VGC27_11390"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0283213.1"
                     /translation="MTENRMKIGFICLGNMGAAIAGNILGAGHQLTVWNRSPAPVAAL
                     VGRGAVAAKTPKDALQGDALFSMLANDAALQEVGLTGPLLEHAAPGLVHVNLATVSIE
                     LARRMAEVHTARGVGYVAAPVFGRPDAAAETKLVVIAAGADKDIARVRPVLEAIGQRV
                     AVVGKAPEQANLFKIAGNFMLAAAIETMGEAVALMRKGGVDPALFIQVLTTTLFAAPA
                     YQGYGALIVAQKYDPAGFKLSLGMKDAGLALAAGNELQVPLPLASLIHDHFIEAMAAG
                     MGEKDWAVLADLAATKAGLPGT"
     gene            1151..2323
                     /locus_tag="VGC27_11395"
     CDS             1151..2323
                     /locus_tag="VGC27_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0283214.1"
                     /translation="MPIGRMGTWPGRVNAAVKENLPSGIRQYITPRMITMLVIVAVVL
                     VLVFGYIKLAPLVSYLMNPGQYAVPPQSVSTVHAQKTLWQTQLRSVGTLHAVEGADLA
                     SEVAGLVTRIGFQPGEDVRAGTLLVQLRDDSEQAAAEEALLTYRRYAALLKTQAISQS
                     SYDSALANLKSTRAAMEKKAIRAPYDGRVGIRQVDVGQYVNAGQVIVTLQQLDPIFVD
                     FQVPQQQLSLLTKGDKVTLVTDAAAGRNFYGEIVAFDPKVDPDTRNVRVRASIRNPDK
                     TLLPGMFATAITNVGSARPLITLPQTAVVYNPYGDTVFVVAKTAQEKGGEQLVAQQRF
                     VTVGETRGDQVAILSGLSTEDIVVSAGQIKLKNGTVVSVNNSVTLPNNPAPTPVDQ"
     gene            2334..>2960
                     /locus_tag="VGC27_11400"
     CDS             2334..>2960
                     /locus_tag="VGC27_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0283215.1"
                     /translation="MHFTDLFIRRPVLSIVISLAIFVIGLRAFTDLPVRQYPLTTNAV
                     VTVSTAYYGASPEVIAGFITSPLENAIAQANGIDYMTSTSTQGVSVIQANLRLNYDAN
                     KALSEIITQVNSVKNQMPADAQQSVINIAVGQSTDAMYISFNSKVLPPNKITDYLIRV
                     VQPQLQAVEGVQQAELLGGKRFAVRAWLKPDKLAAYGLTATDVYAALGS"
BASE COUNT          550 a          944 c          932 g          534 t
ORIGIN      
        1 gatgacggag aaccgcatga agattggctt tatctgtctt ggaaacatgg gcgcggcgat
       61 tgccgggaat atcctcggcg ccggccatca gctcacggta tggaaccgct cgcccgcgcc
      121 cgttgccgct ctcgtcggcc ggggcgcggt ggcggcgaag acgcccaagg acgcgcttca
      181 gggcgatgct ttgttctcga tgctcgccaa cgatgcggcc ttgcaggagg tcgggctgac
      241 cgggccgctg ctggagcatg cggcgcccgg gctggtgcat gtgaatctgg cgaccgtctc
      301 catcgaattg gcccgccgga tggccgaggt tcataccgcg cgcggcgtag gctacgtcgc
      361 cgcgccggta ttcggccgcc cggatgcggc ggcggagacc aagctggtgg tcatcgccgc
      421 cggggcggac aaagacatcg cacgggtgcg gccggtcctc gaagcaatcg gccagcgtgt
      481 agcggttgtg ggcaaggcgc cggagcaggc gaatctgttc aagatcgccg gcaatttcat
      541 gctggctgcg gccatcgaaa ccatgggcga agccgtcgct ttgatgcgca agggaggggt
      601 ggacccggcg ctcttcatcc aggtcctgac caccactttg ttcgccgctc ccgcctatca
      661 gggctatggc gcgctcattg tggcgcagaa atacgacccg gcggggttca aattgagcct
      721 ggggatgaag gatgccggtc tggcgctcgc cgcgggcaat gagctgcagg tcccgctgcc
      781 gctcgccagc ctgattcacg accattttat cgaggcgatg gcggcgggca tgggcgagaa
      841 ggactgggcc gtgctggcgg atctggccgc cacgaaggcg gggctgccgg gcacttaacc
      901 cggattccga tgcatgcgct ggcagcggcc gtcccgcaag ccgctgtcaa catggctttt
      961 caagctgtcc gggcgggtaa ccctaacgaa ttcgtgacac tttgttcgcc cacgcacttg
     1021 tcatttgggg gcggcgagac aatatcctaa ctcaacattc gttggctgtg agaatcacgc
     1081 gcgcaacggt agataccgcc gtaccgcccc gcttccgcgc ggcgtgacgt cttttttgtg
     1141 ggaacggacg atgccgatag gacgtatggg gacctggccg gggcgcgtca acgccgccgt
     1201 taaggagaat ctgccttccg gaataaggca gtacatcacc ccccgcatga tcacgatgct
     1261 ggtgatcgtg gccgtcgtgc tggtgctggt ctttggctac atcaaactgg cccccctcgt
     1321 gtcttacctc atgaaccccg ggcaatacgc cgttcctccg cagtcggtct ccaccgtgca
     1381 cgcgcaaaag acgctctggc agacgcaatt gcgttcggtc ggaacgctgc acgcggtcga
     1441 gggtgccgat ctggcgtcgg aagtcgcggg tctcgtcacg cgaatcggct tccaaccggg
     1501 agaggacgtg cgtgccggta cgcttctcgt ccagttgcgc gacgattccg agcaggctgc
     1561 tgccgaggag gcgctgctaa cttacaggcg ctatgccgcg ctgctcaaaa cccaggcaat
     1621 cagccagtcg agttacgata gcgccttggc caacctgaaa agcacccggg ctgcgatgga
     1681 gaagaaggcc atccgtgcgc cttacgacgg tcgggtcggc atccggcagg tcgatgtcgg
     1741 gcaatatgtg aatgccgggc aagtcatcgt caccctgcag cagctcgacc cgatcttcgt
     1801 cgattttcag gttccgcagc agcagttgag cctgctgacc aagggcgaca aagtcacact
     1861 ggtcaccgac gccgccgccg gcaggaactt ctacggcgag atcgtcgcct tcgatcccaa
     1921 agtggacccc gacacccgca acgtccgtgt ccgggcctcg atccggaatc ccgacaagac
     1981 gctgctgccc ggcatgttcg cgacggccat caccaacgtc ggatcggcgc ggccgttgat
     2041 cacgctcccg caaaccgccg tcgtttacaa tccttacggc gacacggtct tcgtcgtcgc
     2101 gaaaaccgcg caagagaagg gcggcgagca gcttgtggcc cagcagcgtt tcgtcacggt
     2161 cggagaaacg cgcggcgacc aagtcgcgat attgagcggc ctctccaccg aagacattgt
     2221 ggtaagcgcc ggccagatca agctgaagaa cggcaccgtc gtgagcgtca acaacagtgt
     2281 gacgctcccg aacaatcccg cgcccacgcc cgtggaccaa taggccagag gcgatgcatt
     2341 tcaccgacct ttttatccgg cgccccgttc tgtcgatcgt catcagcctg gcgatcttcg
     2401 tgatcgggct gcgtgcgttc acggacttgc cggtgcggca atatcccttg acgacgaacg
     2461 cggtcgtcac ggtttcgacg gcctattacg gcgccagtcc cgaggtcatt gccggcttca
     2521 tcacctcgcc gctcgaaaac gccatcgccc aggccaacgg catcgactac atgacgtcga
     2581 ccagcaccca gggcgtctcg gtcattcagg cgaaccttcg tctcaactac gacgccaaca
     2641 aggcgctctc cgaaatcatc acccaggtca attcggtcaa gaaccagatg ccggccgacg
     2701 cgcagcagtc ggtcatcaac atcgcggtcg gtcaatcgac cgacgcgatg tacatcagct
     2761 tcaacagcaa ggtgctgccg ccgaacaaga tcacggacta tctgatccgc gtggtgcagc
     2821 ctcaattgca ggccgtcgag ggcgtgcagc aggcggagct tctcggcggc aagcgctttg
     2881 ccgtccgcgc ctggctgaag ccggacaagc tggcggccta cggcctgacc gccacggacg
     2941 tttacgcggc gctcggcagc
//
LOCUS       DASWPU010000541         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_84489,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000541 DASWPU010000000
VERSION     DASWPU010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84489"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VGC27_11405"
     CDS             complement(<1..858)
                     /locus_tag="VGC27_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283216.1"
                     /translation="MLALNLHPPGTPADIVMTASYPASCGNPITCNRPTSVTDARGYR
                     TDYTYDATHGGVLTATRSAPSGAPPVGTGTRPQTRYTYGSFYAWYKNSSGNIVQGTAP
                     VYKLTQTSTCATLASCTGTGDESRSTTAYQAGSASTASNILPLTVTTGAGNGSLSATT
                     TTTYDQVGNVIAVDGPLAGDGDKAAYRYDALRRKTGEVAAAYTASDGQTKRRATRMTY
                     HLNGQVQTAEQGAVAGLSDSDWSAFAPLTWSVNDFDALGRVIKTRQQNSAGIAAVTQF
                     SYDTENRVVC"
     gene            complement(957..1430)
                     /locus_tag="VGC27_11410"
     CDS             complement(957..1430)
                     /locus_tag="VGC27_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283217.1"
                     /translation="MSSTEARPAGMWIIAFALLGNGALGAWLLLEQQFGQPTFLSFPM
                     LTGGSRIETLIAYMCAGLMCGCFLIAGIALLFSRKGAIPIYLAGLIFYAGYITAAFLI
                     FFVRGAWLSIVAGDFSVGDALFVLVFLVGGCALSFVVARAVYNYLRSLSRSGLLR"
BASE COUNT          324 a          477 c          457 g          288 t
ORIGIN      
        1 gcagacgacg cggttttccg tatcgtagct gaactgcgtc acggccgcga tcccggcact
       61 gttctgctgg cgggtcttga tgacgcgccc tagcgcgtca aaatcgttca cgctccaggt
      121 gagcggcgca aaggcgctcc aatcggaatc ggagaggccg gcgacggcgc cctgctcggc
      181 cgtctgaacc tggccgttga ggtgataggt catccgcgtg gcgcgccgct tggtttggcc
      241 gtcagatgcg gtgtaggccg ctgcgacctc gcccgtcttc cggcgcaggg catcataacg
      301 atacgccgcc ttgtcgccgt cgcccgccag cggcccatcc accgcaatga cgttgcccac
      361 ctggtcatag gtggtggtgg ttgtggcact cagcgaaccg ttgcctgcgc ccgtggtcac
      421 ggtaagcggc aggatattgc tggccgtgct cgccgagccg gcctgatagg cggtggtcga
      481 acggctttca tcgcccgtgc cggtgcatga tgcgagcgtg gcgcatgtcg aggtttgcgt
      541 cagcttgtac accggcgcgg tgccctgcac gatgttcccg gaactgttct tgtaccaggc
      601 ataaaacgag ccataggtgt agcgcgtctg cggtctcgtg cccgttccga ccggcggcgc
      661 gcccgaagga gccgaccgcg tggccgtcaa gacgccgcca tgggtggcat cgtaggtata
      721 gtccgtgcga tatccgcgtg cgtcggttac cgacgtcggc ctgttgcagg tgatgggatt
      781 gccgcagctc gccggatagg aagctgtcat gacgatgtcg gccggcgtgc cgggcgggtg
      841 cagatttaga gcaagcaccg gagcatatcc cgcagtggaa tcgatgacgc cgcgccgcga
      901 tgtcgatcag attcagagcg ccagaacagt cccagcgggg cgcgattccg gcgcggtcat
      961 cgtagtaatc cgcttcgtga aaggcttcta aggtagttgt acacagcgcg agccacgaca
     1021 aaactgagtg cacatccgcc caccaggaat accaatacaa acagcgcgtc accaaccgag
     1081 aagtcaccag caacgatact cagccacgcc ccgcgcacaa aaaaaatcag gaacgctgct
     1141 gtgatgtatc cggcatagaa gatcaggcca gctaagtaga tcgggattgc cccctttcga
     1201 gagaacagca acgcgattcc tgctatcaga aaacagccac acatcagccc cgcacacata
     1261 taggcgatta gggtctcgat cctgcttccg cccgtcaaca tcggaaacga caaaaaagtc
     1321 ggttgaccaa actgctgctc cagtaacagc catgctccaa gggcgccatt acccagaaga
     1381 gcgaaagcaa tgatccacat tccagctggc cgcgcttcgg tactcgacat gatctttcct
     1441 cccccaagta gcttcagtta cagtaggtca tctgcatgag ttcggaagaa ctgacagatg
     1501 ctccagcctt taatccaagg ccaccaccac caccgccacc gagtat
//
LOCUS       DASWPU010000542         4312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_35813,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000542 DASWPU010000000
VERSION     DASWPU010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35813"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VGC27_11415"
     CDS             complement(<1..426)
                     /locus_tag="VGC27_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283218.1"
                     /translation="MITEASVIRQPVGEGCAPVPSIAFWALKTALLWFALLASAVIAS
                     KCVTIDLPQPPKDGPLSIESAFLLVNGLVAIALGLVASRARVSGIGRAVLLFVAMFGI
                     STAMMQIETLYFNESVRMPPDVLVGLVEQSAIISFIVAIV"
     gene            604..2571
                     /locus_tag="VGC27_11420"
     CDS             604..2571
                     /locus_tag="VGC27_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEY0283219.1"
                     /translation="MSTPSAIKRVTELTLRGLILGVLITLVFTAANVYLGLKVGLTFA
                     TSIPAAVISMAVLAAFKDSSILENNIVQTVASAAGTLSAIIFVLPGLVIVGWWTGFPF
                     WTSFWICASGGVLGVLFTIPLRRAMVTTSDLPYPEGVAAAEVLVVGDQARQAAAGGAS
                     TETKEGLLAVVYGSIASLGLAAFTATNIGAAAVTGYFRLGRGAGGYDMAFSLALLGAG
                     YLVGLSVGAAMFLGLVIAWGAALPILATFIPQGTLPLGDYVHSIWLHKVRFIGAGTIA
                     VAAIWTLTKLAGPVVRGVIQTMAASRAKVEAGDTRDVDMSPLWIGLLTIACLAAIAWL
                     LQTFLATTPLADKTTNLVAAALPFIFFGGFLVAAICGYMAGLIGASNSPLSGVGILAV
                     ICCAVILIAAANPTAEMRSALVAYALFATAIVFAIATISNNNLQDLKTGQLVGATPRA
                     QQWALIVGVIAGAAVIPPVLNLLAQAYGFAGAPRLASAVAQPLAAPQAGLISALAQGV
                     IEQKLDMEMIGLGAAIGVGMIALDQFLGMRKWLRLPPLCVGIGIYLPMSATLPVVTGA
                     VIGYAYDRWSNRLKSQEHAKRLGVLVASGMIVGESLFGVLLAGLIVAFSTDAPLGLVA
                     GDFAPAPYIGLAAFAALIALLYGWMMRRAKA"
     gene            complement(2568..3368)
                     /locus_tag="VGC27_11425"
     CDS             complement(2568..3368)
                     /locus_tag="VGC27_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0283220.1"
                     /translation="MPDEPSFEPHRFRSTVPYYARYRLSYPDLLIRRVAALVGLKPGD
                     LVLDLGCGPGFLAVPFARAGMAVTAVDPEPEMVAAAREAAKEAAVTVDVRQGSSFDLP
                     AGIGPFALVTMGRSFHWMDRAGTAKRLDGLVVPGGALALFNDEHLNTVENLWRNVLLE
                     DVGARYGADEAPHRAARKNPDYRTHESILLDSPFCVLETAGVIVRRELSVDEIVGYAF
                     SLSVTAQQQLGDRAEAFEADLRAELAKLAPDGRLREIAEMRALIARRS"
     gene            3438..4139
                     /locus_tag="VGC27_11430"
     CDS             3438..4139
                     /locus_tag="VGC27_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0283221.1"
                     /translation="MANIALVDDEQGILTSVSMLLEKEGYHVRTFSDGASALTALSAT
                     PPDLAILDIKMPRMDGMELLRRLRQNVDLPVIFLTSKDEEIDELMGLNAGADDYIRKP
                     FSQRLLLERVKAVLRRAEGHKGGGTGPDSKKEALVRGKLALDPLRHECTWEGKPVRLT
                     VTEFLILQSLAQRPGFVKSRDNLMDAAYDDEVYVDDRTIDSHIKRLRKKFKQVDDDFE
                     SIETLYGVGYRYRET"
BASE COUNT          794 a         1378 c         1342 g          798 t
ORIGIN      
        1 cacgatggca acgataaacg atatgattgc ggattgttcg accagcccga caagcacatc
       61 cggcggcatg cggacactct cgttgaagta aagagtctcg atctgcatca tcgcggtcga
      121 gatgccgaac attgcgacga agagcaggac tgcgcgccct atgccgctca cgcgcgcccg
      181 ggaggcgaca aggccaagcg cgatcgcaac cagcccgttc accagcagaa aagcgctctc
      241 gatggatagc ggaccgtcct tcgggggctg cggcagatcg atggtgacgc acttggaagc
      301 gatgacggcg gaagcgagaa gcgcaaacca tagaagtgcg gtcttgagcg cccagaaggc
      361 gattgacgga accggagcgc acccctctcc aacaggctga cggatcacgg atgcttcggt
      421 gatcatgatt tcctccctcc aatagcaaca tccttatact aaaagtactt tattttgtaa
      481 agcacttcat tatcacttcc ttgtagacca agctggcgcg taccaacgat ttgaacttaa
      541 agggcctttc gccatccgcc aggcgtggca ctctcgctta gcgactcgaa cacgagggaa
      601 tccgtgagca cgccgtctgc catcaaacgc gtcaccgaac tcacccttcg cgggctcatt
      661 ctcggcgtcc tcatcaccct cgtgttcacg gcggcgaacg tctatctggg cctcaaggtc
      721 gggctgactt ttgccacctc cattcccgcg gccgtgatct cgatggccgt gctggcggcc
      781 ttcaaagact cgtccatcct ggaaaacaac atcgtgcaga ccgtcgcctc ggcggcgggc
      841 acgctgtccg ccatcatctt cgtgctgccg ggtctggtga tcgtgggctg gtggacgggc
      901 tttccgttct ggacctcgtt ctggatctgc gcctcgggcg gcgtgctggg cgttctgttc
      961 acgatcccct tgcgccgggc gatggtgacg acctccgacc tgccctatcc cgaaggcgtg
     1021 gcggcggcgg aagtcctggt ggtcggcgac caagcacggc aagccgcggc aggcggagcg
     1081 tccaccgaga cgaaagaagg cttgctggcc gtcgtctacg gctcgatcgc ctcgctgggc
     1141 ctggcggcgt tcacggctac gaatatcggt gccgccgcgg tgacaggtta cttccggctc
     1201 ggacgcgggg cgggaggcta cgacatggcc ttttcgctgg cgcttctcgg cgccggatat
     1261 ctggtgggcc tttccgtcgg cgcggcgatg ttcctcggcc ttgtgatcgc ttggggtgcg
     1321 gcccttccga tcctggcaac gttcataccg caaggcacgc tgccgctcgg cgattacgtt
     1381 cactccatct ggctgcataa agtgcgcttc attggagccg gcaccatagc cgtggccgcg
     1441 atctggacct tgacgaagct ggcgggcccg gttgtacgcg gtgtcatcca gaccatggcg
     1501 gcctcgcgcg caaaggtcga agcgggagac acgcgcgacg tcgacatgtc gccgctgtgg
     1561 atcgggctgc tcacgatcgc ctgcctggcc gccatcgcct ggctgctgca gacgttcctt
     1621 gccacaacgc cgctcgccga caagacgacc aatcttgtgg ccgctgcgct tcccttcatc
     1681 ttcttcggtg gcttccttgt ggcggcgatt tgcggctaca tggcggggtt gatcggcgcc
     1741 tccaacagcc cgctctcggg cgttggaata ctggccgtca tctgttgcgc cgttatcctg
     1801 atagcggcgg caaatccgac ggccgagatg cgcagcgcgc tcgtcgctta tgccctgttc
     1861 gcgaccgcca tcgttttcgc catcgccacg atttccaaca acaatttgca agacttgaag
     1921 accggccaac tggtcggcgc gacgccgcgg gcccagcaat gggcgctgat cgtcggcgtg
     1981 atcgcggggg ccgccgtgat cccaccggtg ctgaacctgc tggcccaggc ttacggcttc
     2041 gccggcgcgc cgcgcctggc aagcgccgtg gcacagccgc tagcggcgcc gcaggcaggc
     2101 ttgatatcgg cgctggcgca aggcgtcatc gagcagaagc tcgacatgga aatgatcggc
     2161 ctgggggccg cgatcggcgt cggcatgatc gcgctcgacc aattcctggg catgcgcaaa
     2221 tggctgcggc tgccgccgct gtgcgtgggc atcggcattt acctgccgat gtcggcaacc
     2281 ctgccggtcg tgacgggggc cgtgatcggg tacgcctatg accgctggtc gaaccgcctg
     2341 aagtcgcaag agcatgccaa gcgactcggt gtgctggtcg cttccggaat gatcgtgggc
     2401 gagagtctgt tcggcgtgct gctggcggga ttgatcgtgg cgttttccac cgacgcgccg
     2461 ctggggctgg tggcggggga tttcgcgccc gcgccttata tcgggctggc ggcgttcgcc
     2521 gccctgatcg cgctgttgta cggctggatg atgcgccggg cgaaagctta agatctacgt
     2581 gcgatcagcg cgcgcatctc ggcgatctcc cgcaggcgcc cgtcgggcgc cagcttggcc
     2641 agttccgcgc gcaagtcggc ctcgaacgcc tcggcgcggt cgccaagctg ctgctgcgcc
     2701 gtgaccgaca aagaaaacgc atacccgacg atttcatcca cgctaagctc ccgccgtacg
     2761 atcacgcccg cggtttcaag cacgcaaaat ggcgaatcga gcagaatcga ttcatgcgtg
     2821 cggtagtcgg ggtttttacg tgcggcgcga tggggcgctt cgtccgctcc gtaacgggcg
     2881 ccaacatcct ccagcagcac gttgcgccag aggttttcca ccgtattgag gtgttcgtcg
     2941 ttgaacagcg ccaaggcgcc gcccggcacc accagcccat cgagccgctt tgcggtcccc
     3001 gcccgatcca tccagtggaa cgaccggccc atcgtgacca gggcaaacgg cccgattccg
     3061 gcaggcagat cgaaggaact gccctggcgg acatcgaccg taaccgccgc ctctttcgcc
     3121 gcttcccgcg ccgcggccac catttccggc tccgggtcga cggcggtcac agccattccc
     3181 gcccgggcga agggcaccgc cagaaatcct ggcccacagc ccaaatccag caccaaatcg
     3241 cccggcttca ggcccacaag cgcggcaacg cgcctgatca gcaaatccgg ataagacagg
     3301 cgatagcgtg cataataggg cacggtggaa cggaaccggt gcggttcgaa gctgggctcg
     3361 tcgggcatgg ctcacctaac gttttgattt gaaggctgcg ctcttatact aaggaagcta
     3421 cagccgggct ggaaaaaatg gcgaacatcg cgctcgtaga cgacgaacag ggcattctga
     3481 cttcggtatc gatgctgctg gagaaggagg gctatcacgt ccgcaccttc agcgacggcg
     3541 cttccgcgct gaccgcactc agcgccacgc cgccggacct tgccatcctc gacatcaaga
     3601 tgccgcgcat ggacggcatg gaactactgc gccggctgcg tcagaacgtg gatctgccgg
     3661 tgattttcct cacctcgaag gacgaggaaa tcgacgagtt gatgggactc aacgccggag
     3721 ccgacgatta catccgcaag ccgttctcgc agcggctgct gctcgaacgc gtcaaggccg
     3781 tgctgcgccg cgccgaaggg cacaagggcg gcggcaccgg tccggacagc aagaaggaag
     3841 cgctggtgcg cggcaagctg gcgctcgatc cgctgcgcca cgaatgcacc tgggaaggca
     3901 aaccggtgcg gctgaccgtc accgaattcc tcatcctgca aagcctggcg cagcgcccgg
     3961 gcttcgtcaa aagccgcgac aacctgatgg atgcggccta tgacgacgaa gtgtatgtcg
     4021 acgaccgcac gatcgacagc cacatcaagc gcctgcgcaa gaagttcaag caggtcgacg
     4081 acgatttcga gtcgatcgaa acgctgtacg gagtcggcta tcgctaccgt gagacgtaaa
     4141 atagcgatat tagagcatga tctcctggga tggaagccga aggcgagctt cgaaaaatga
     4201 cgctgtccga agacacgcgc ccttcgcgcc attccaggct gcgccagcgc atcatcgtca
     4261 tcaacatcat cgtcttcctg ctcttgatcg ccggggtgct ggcggtgcaa tc
//
LOCUS       DASWPU010000543         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_72204,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000543 DASWPU010000000
VERSION     DASWPU010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72204"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..>1560
                     /locus_tag="VGC27_11435"
     CDS             271..>1560
                     /locus_tag="VGC27_11435"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0283222.1"
                     /translation="MAISFARWFITVSAAALAAAAAHAEDAGPGETVVVTGTAASGYR
                     VSDFAMGPLGDRAVLDLPYSVQTVSQDLIRNAGVDENWELLKYLPSTQIEYRGGSEVG
                     RPQARGFEADLLGNTRIDGFAVQSHIPQPVELTDHVDVFQGLSGALYGPMNPAGVFNY
                     VLKRPVDGMHFELSGTYRSDENATVRADAGGRIGGSTSFGFRGNFAYTDGKTYVDGSD
                     LHREVEGIAFDVKLPFGTTIEANAAQYVYDRTGYPGSFSVATGAAAQIPDANDIDPSL
                     PGYGYSWAGVKTNIKYYGVRATQLFGDGWKLTGGFLWQNVTRAMHSVGNALSADGTRY
                     RQSGSEAFTHWNTLANQLYLNGKFSLLGLDNEITLGTNGYVNPGYAAKNSAAASTSAG
                     TAANPCSAIAAATCTLAEPEWNGFGSFSRTGLGNRYQT"
BASE COUNT          288 a          505 c          484 g          283 t
ORIGIN      
        1 ccgccttcgc agatgattga caatcaaggc acttcgacaa gatcatgtcc gcgttcgaga
       61 acggtgcttt ccttctcgaa cgtgttgccg gggcgcctgc gcgtctcaag gcaacggaaa
      121 tcggcctgag ccggtgcatc cgacgcgtat tgccgcttcg tcgtaagcgg acaatgccgg
      181 acgacacgga caaggccgcg acagcccaaa caccgtgttt tcccccgtgc cggatgccgt
      241 tcgcaacgtc agtggcaaga ggggtagacc atggccattt cattcgcgcg ttggttcata
      301 accgtgtctg cggcagcgct cgctgctgct gcggcacatg ccgaggacgc cggccccggc
      361 gaaaccgtgg tggtgaccgg aaccgccgcc agcggctatc gcgtctccga ttttgccatg
      421 gggccgctgg gcgacagggc cgtgctcgat cttccctatt cggtgcagac ggtttcgcag
      481 gatctgatcc gcaatgccgg tgtcgatgag aattgggagc tgttgaagta cctgccgtca
      541 acccagatcg aatatcgcgg cggctcggaa gtggggcggc cgcaggcgcg cgggttcgag
      601 gccgatcttc tcggcaatac ccgtatcgac ggcttcgccg tgcagtcgca tatcccccag
      661 ccggtggaac tgaccgacca tgtcgatgtg ttccaggggc tttccggcgc gctctatggg
      721 ccgatgaatc cggcgggcgt tttcaattac gtgctgaagc gccccgtcga cgggatgcat
      781 ttcgaactgt ccggcaccta ccgctccgac gagaacgcca cggtgcgcgc cgatgcgggc
      841 gggcggatcg gcggcagcac ctccttcgga tttcgcggca acttcgccta taccgacggc
      901 aagacctatg tcgacggcag cgacctgcat cgcgaagtgg aaggtatcgc gttcgatgtc
      961 aagctgccgt tcggaaccac gatcgaggcc aacgccgcgc aatatgtcta cgaccgcacc
     1021 ggctatccgg gctcgttctc ggtggcgacc ggcgcggcgg cgcagattcc cgacgccaac
     1081 gacatcgatc cgtcgctgcc cggttacggc tacagttggg cgggcgtgaa gaccaacatc
     1141 aaatattacg gcgtgcgcgc aacgcaattg ttcggcgacg gctggaaact caccggcggc
     1201 tttctctggc agaacgtcac ccgcgccatg cacagcgtcg gcaacgcgct gtccgccgac
     1261 ggcaccagat accgccagag cggttccgag gccttcacgc attggaacac gctggccaac
     1321 cagctttatc tcaacggcaa gttctcgctg ctcggcctcg acaacgagat cacgctcggc
     1381 accaacggct acgtcaatcc cggttatgcg gccaagaata gcgctgcggc ctcgaccagc
     1441 gccggcacgg cggcgaaccc atgttcggcg atcgcggccg cgacctgcac cctggcggag
     1501 cccgaatgga acggctttgg cagtttttcc aggaccggac tcggcaaccg ctatcagacg
//
LOCUS       DASWPU010000544         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_90270,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000544 DASWPU010000000
VERSION     DASWPU010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90270"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VGC27_11440"
     CDS             complement(<1..629)
                     /locus_tag="VGC27_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEY0283223.1"
                     /translation="MAELFEHELDAFPVISGTAANALALAALVPPHGAIFCHSESHIA
                     VDECGAPEFFTHGAKLVPLEGENGKLVPETLERALGRFQKGFVHHVQPAALSLTQSTE
                     LGTVYRPGEIAALSALAHAHGMTVHMDGARFANALATLGCSPADATWRAGVNAISFGA
                     TKNGALGAEAVIFFGSAEARDFEYRRKKAGHLLSKMRFVSAQLDAYLTGT"
     gene            complement(700..1140)
                     /locus_tag="VGC27_11445"
     CDS             complement(700..1140)
                     /locus_tag="VGC27_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0283224.1"
                     /translation="MRYLHSMLRVANLETALDFFCSKLGFREVRRTESEKGRFTLVFL
                     AAPGDWQRAASNGAPLVELTYNWDPEDYTGGRNFGHLAYAVDDIYATCEKLMAAGIVI
                     NRPPREGRMAFVRTPDGISIELLQDGESLPAKEPWLSMPNTGSW"
     gene            1690..2244
                     /locus_tag="VGC27_11450"
     CDS             1690..2244
                     /locus_tag="VGC27_11450"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0283225.1"
                     /translation="MAALAASTAIVIGFAWALSTGLAQTMVEKGFDVLKAEVLPEKLP
                     DKTPPPPPPDLKAPPPPYVPPPDIIIQTDVAPSANTITAQSKVASAPAISSPASIGRP
                     HTCMQNYPAISQRLGEEGVTLLAFTVTAEGGVANITVAKSSGSERLDNAAISCASSWR
                     YKPAIEAGHPVAVSWKTNVVWKLK"
     gene            2314..>2570
                     /locus_tag="VGC27_11455"
     CDS             2314..>2570
                     /locus_tag="VGC27_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283226.1"
                     /translation="MISKHLTIAAAFVLLTGFGVGPVLAQDTGGAPAPAATTTDTADS
                     ADPAEAGPTIIKAPTTAAAEQENPYGLADIIKKKNPVSYSVL"
BASE COUNT          484 a          802 c          797 g          487 t
ORIGIN      
        1 gtgcccgtga gataagcgtc gagctgagcc gagacgaagc gcatcttgga caggagatgg
       61 cccgccttct tgcgccggta ttcgaagtcg cgcgcctcgg ccgagccgaa gaagatcacc
      121 gcctcggcgc cgagcgcgcc gttcttggtg gcgccgaagg atatcgcgtt cacgccggcg
      181 cgccaggtcg cgtcggcggg actgcatccc aaagtggcca gtgcattggc gaagcgcgcg
      241 ccgtccatgt gcaccgtcat gccgtgggca tgcgccagcg ccgaaagcgc cgcgatctcg
      301 ccgggccgat agaccgttcc cagttcggtg gattgagtga ggctgagcgc cgccggctgg
      361 acgtgatgca cgaaaccctt ctggaaacgg ccgagcgccc gctccagggt ctcgggaacc
      421 agcttgccgt tctcgccctc cagcggcacc agcttggcgc cgtgggtgaa gaattcgggc
      481 gcgccgcatt cgtcgaccgc gatgtggctt tcgctgtgac agaagattgc gccgtgcggc
      541 ggcaccagcg ccgccagcgc cagcgcattg gccgccgtgc cgctgatcac ggggaaggcg
      601 tccagctcgt gctcgaacaa ttcggccaag cgcgcgcgca cctaggcggt cagcgcgtcc
      661 tcgccgtagg aattatcgga ggcgaagttc attcgggttc taccagcttc ctgtattcgg
      721 catcgacagc cagggttctt tcgccggcag cgactcgccg tcctgcaaga gttcgatcga
      781 aatgccgtcg ggcgtgcgca cgaacgccat gcgcccctcg cgcggcggcc ggtttatgac
      841 aatgcccgcc gccatcagct tttcgcatgt cgcataaatg tcgtcgacgg cgtaggcgag
      901 gtgtccgaaa ttccggccgc cggtgtaatc ctcgggatcc caattgtaag tgagctcgac
      961 cagcggagcg ccgttgctcg ccgcgcgctg ccagtcgccg ggcgccgcga gaaaaaccag
     1021 cgtaaaccgg cctttctcgc tctccgtccg gcgaacctcg cgaaagccga gtttgctgca
     1081 aaagaagtcg agcgccgttt caaggttggc gacccgcaac atgctgtgca ggtatcgcat
     1141 tgcgtactcc cgttttcaca attcaagggg caggcggaac attctccgca ttcgccgtca
     1201 agagaactca tattgcagcg cggcatagat ttctgcacca tcacgttgcg gttgtggtaa
     1261 gctgttttgc gcaatgcgct tttgggcgcc tcggcgcggc cgcgccgatt ggtcgcgtag
     1321 tgcggaccta tgtgtcacgc ggcaaaacgc aagttttcct cttgcgtttt atgccacaat
     1381 gttatgtgac gctgcactga tgggaagcga ctcgatggtt tgcgcgttgt taaccaaact
     1441 aaacgagaat cagcatttct tgtcagaccc tctggaccgg ttcgcgaacg atgtttacga
     1501 ttagttttta cgatagcgaa gagcgctcgc cgaaacgggc gcctggtggg gggcgcgtga
     1561 gcttggtccg caatggagca gcccggcgcg tttgcgtcgg gttcagtttt atcaggtgcc
     1621 ggtagatcca tggaacagcc aatacacaca attcgcgcca ttcatacatc ttggttggcg
     1681 ccaaaccgta tggcggcgct ggccgcttcg accgccatcg tcataggctt cgcttgggcg
     1741 ctgtcgacgg gtctggcgca gacgatggtg gaaaagggtt ttgatgtcct caaggccgag
     1801 gtgctgccgg aaaagcttcc ggataagacg ccgccgccgc cgccgccgga cctgaaggct
     1861 ccgccgccgc cctacgtgcc gccgcccgac atcatcatcc agacggatgt cgctccgagc
     1921 gccaacacga ttacggcgca gagcaaggtg gcgtccgcgc cggcgatctc ttcgcccgca
     1981 tcgatcggca ggcctcatac ctgcatgcag aactatccgg cgatttcgca gcgcctgggc
     2041 gaagaagggg tcaccctcct tgccttcacc gtcacggcgg agggcggcgt cgccaatatc
     2101 acggtcgcca agtcgagcgg ttcggaacgc ctggacaacg ccgcgataag ctgcgcgtcg
     2161 agctggcgct acaagccggc catcgaagcg ggccacccgg ttgcagtttc ctggaagacg
     2221 aacgtggtgt ggaaactcaa gtgaaacgcc actgagagat acaagagata caacgttacg
     2281 gtttaattac tacagctacg gagtagtact gacatgatct cgaaacacct gacgatcgca
     2341 gccgctttcg tccttcttac cggtttcggc gtcggccctg tgctggcgca ggatacgggc
     2401 ggcgccccgg cgccggccgc aacgacaacc gatacggccg attcggccga tcccgcggaa
     2461 gccggtccga ccatcatcaa ggctcccacc accgccgctg cggagcaaga aaatccctac
     2521 ggcctggccg acatcatcaa gaagaagaac ccggtctcgt attcggtgct
//
LOCUS       DASWPU010000545         3613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_47653,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000545 DASWPU010000000
VERSION     DASWPU010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47653"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VGC27_11460"
     CDS             complement(<1..481)
                     /locus_tag="VGC27_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283227.1"
                     /translation="MSRIGNIKLRHAGFGLAVFLGAMALVAGVFLVAESERSFESGRW
                     EARLRAGGVQPAARVGDWLTDSRRQLSAVALNPTVQIYLSEVAAADFDPRGVPEGEAK
                     AAFLTSYITSLSGRGAFAPDIPGNRGAAASGAVQSATGLAVLDARRRLVASTLGYKPQ
                     "
     gene            614..1321
                     /locus_tag="VGC27_11465"
     CDS             614..1321
                     /locus_tag="VGC27_11465"
                     /inference="COORDINATES: protein motif:HMM:NF017815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEY0283228.1"
                     /translation="MRSVSILKMMVVGLLLMAPTIAFANSVFKSGIAGSHGFLFFPFW
                     QQVLTDMAAAQPNATFVATRTGPAQAQPCANERTCIPAAWISFLDGIRNKPRLAQLNA
                     VNLWANAKPYVEDWAGWRVADYWETPGEFLARGGDCEDYAITKYFSLVRLGFSPDDLR
                     IVIVNDTNLNVFHAVLVARVEGGIWLLDNQLQQTVPMDIAVQYVPVYSLNERGWWIHS
                     VPKIKLGNVTIAAGGAN"
     gene            2274..2777
                     /locus_tag="VGC27_11470"
     CDS             2274..2777
                     /locus_tag="VGC27_11470"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HEY0283229.1"
                     /translation="MRRCASITTALTLLSVMGTIPGYAATTTTTFAVTATVLSGCLVT
                     ATNLIFGNYSSVSATPTDATSTVTATCSLGVSYTIAADAGIGAGATVAARVMTGTIGG
                     GLLNYLMHSDAARTALWGNGTLGTATVGGTAALLPQSYTVYGRIPINQYPAAGLYTDL
                     VTVTLTY"
     gene            2789..3532
                     /locus_tag="VGC27_11475"
     CDS             2789..3532
                     /locus_tag="VGC27_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEY0283230.1"
                     /translation="MRKLAALAMGIMTAGGLAVITAKPAIAGALEVAPTTINFPAGTD
                     RAVIYVTNHGAEAVVIQIQGFDWRQTGGIDQMEKSDALILSPPMARLLPEKRQTIRLA
                     LKSNRASTNEQAFRLILSELPDPQRQPQDPPGVQVLLQLSLPVFAEGSDPRPAPLTWS
                     AAPSTKGVSLAVHNNGTTHVKLLDLVLTTSEGTHIPIAPGTMSYVLAGGSHHWESAIP
                     NVKAGDRLHITGHEDSRGTMIDAPIIVTP"
BASE COUNT          740 a         1139 c         1013 g          721 t
ORIGIN      
        1 gctgcggctt atagccgagc gtggacgcaa cgagccgacg gcgtgcgtcg agtaccgcaa
       61 gccctgtcgc agactgcacg gcgccgctcg cggccgcgcc gcgattgccc ggtatgtccg
      121 gcgcaaaggc gccgcgcccg ctcagactcg ttatatagct cgtgagaaac gcggctttcg
      181 cttctccttc cggcaccccg cgagggtcga aatccgctgc ggcgacctcg gacagataga
      241 tctgcacagt cgggttgagc gcgacggcac tcaattggcg gcggctgtcc gtcaaccaat
      301 cgccaaccct agcggccggt tgcacgccac ccgcacgcag ccttgcctcc caacggccgc
      361 tttcgaagct gcgctcgctt tcggcaacaa ggaaaacacc cgcaaccagc gccatagcgc
      421 ctaagaaaac ggctaggccg aatcccgcat ggcgcagctt gatattgccg attctcgaca
      481 tgcgccctct gccttcataa ccaggataac aaagtgttgc cgaagcctga acaattccgc
      541 gcgattctac aattggacct taatgggaca agatcacact gtcaccgacg gaacctattg
      601 aagcgccggt gtcatgcggt cagtttcaat cctaaagatg atggtcgtcg ggctgctgct
      661 gatggcaccg accatcgcct tcgcgaattc ggtgttcaag agcggaatcg cagggtcgca
      721 cggctttttg ttctttccgt tctggcagca agtattgacc gatatggcgg cggcgcagcc
      781 gaacgccacg tttgtcgcaa cgcgcacagg tccggcgcag gcgcagcctt gcgcaaacga
      841 gagaacctgc attccggccg cctggatttc gtttctcgac ggcatccgaa acaaaccgcg
      901 gctggcgcaa ctgaacgccg tcaatctgtg ggcaaacgca aaaccctatg tcgaggattg
      961 ggccggctgg cgcgttgcgg attattggga aaccccaggc gagttcctcg cccggggcgg
     1021 cgattgcgaa gactacgcga tcaccaagta tttcagcttg gtgcggctcg gcttctcgcc
     1081 ggacgatctg cgcatcgtca ttgtcaacga taccaaccta aacgtcttcc atgccgttct
     1141 cgttgcgcgc gtcgaaggcg gcatttggct gctcgacaac cagcttcaac agacggtccc
     1201 gatggacatc gcggtacaat atgtaccggt ctattcgctc aacgagcgtg gctggtggat
     1261 acattcggtt cccaagatca agcttggaaa cgtcaccatc gcggccggcg gggcgaacta
     1321 aaccgggcgc acggagcgtc ggcaaacacc atagtcggcc ggcgcgccat ccgtctatct
     1381 gcgtaatttc cccgcgctac cggcgtcgag cttcgcgccc gtggccattc cgcgctgcgc
     1441 tcgtgttcaa attgggctcg ggactcattg attgatccag aagacgacag ttgcggtaat
     1501 acagattgcc gagaagaatg tgtgtgcgcc cgattgcaac gccttcgccg ctccggcgtg
     1561 taaatggagg gcgccgccgg ggccgaaagc accgaggaaa tccgttgcga tgagagcgtt
     1621 accagccggt taatccggcc ggttcgccgg cctgcggccc agcgatgggc gcgcagcttg
     1681 gcgatcagcg tgccggccgc cggttcgccg aggatgaagc gcagcgccaa ttccgacagg
     1741 ctcttccctt ccgcgaggac tggcgcatcg aaagggtgac ggccaagcaa gcccggcttt
     1801 ccgccgcctc aggccatatc gtcttgctcg gtcttgaccg catccaaagc ttctatactg
     1861 acccaaatcg agcggatcgc gggctaaact atgggcttct tacccttcat gtccaaataa
     1921 tcgtgagagg ctggcctatg tcaaaaccaa tcttcggctc ggccaatggc ctcttcaacc
     1981 caaagaggcc ataaggaaat ctcggcgttt tacatcatgg tatctataat attatacaag
     2041 aatagggatt taatacctag tttgacagta acatttccta tcacttcttc gaatttcatt
     2101 attttctctt cattcttcta tgcctacata taaggttgtg cgttgtagtt ggactcctat
     2161 aggttgccgt caaggcattt tttgctgtga gtcctgagca atcctgccgc tacagccaga
     2221 tagaggactt gcaaatgttt tacgcaaact ttccatcgcc tcgcggcaga acgttgcgcc
     2281 gctgtgcgag catcacgacg gccctgacac ttctttccgt catgggcaca atccccggct
     2341 atgcggcaac caccacaacc accttcgccg tgacggcgac cgtcctttcc ggttgcctgg
     2401 tcaccgcgac gaatttgatc ttcggcaact attccagcgt ctccgccacg ccgacagatg
     2461 cgacaagcac agtgacggcc acctgctcgc tcggcgtctc ctataccatc gccgccgatg
     2521 cgggcatcgg agcgggcgca acggttgcag cccgtgtcat gaccggcacc atcggggggg
     2581 gattgctgaa ctatctgatg cattcggatg cggcccgcac cgccttgtgg ggcaatggaa
     2641 cgttgggaac agccaccgtc gggggcacgg cggcattgtt gccgcagtcc tatacggtct
     2701 acgggcgcat accgatcaat caatatcctg ccgccggcct ctataccgac ctggtcaccg
     2761 tgacactcac ttactaaggc tatgggcgat gcgtaagctt gccgcattgg ctatgggaat
     2821 catgacggcg ggcgggcttg cggtcatcac cgccaagccc gccatagccg gcgcgcttga
     2881 agtcgccccc accacgatca atttcccggc cggaaccgac agagcggtca tctacgtcac
     2941 caaccacggg gcggaggccg ttgtcatcca aatccaggga ttcgattggc gccaaaccgg
     3001 cggcatcgac cagatggaaa aaagcgacgc gctgatactc agcccgccga tggcacgcct
     3061 gctgcccgaa aagcgccaaa ccatccgctt ggcgctcaag tcaaaccgcg cctccacaaa
     3121 cgaacaagcc ttccgcctta tcctcagcga acttcccgac ccgcagcgac agccgcagga
     3181 tcccccgggg gttcaggtcc tgctgcagtt aagcctcccc gtctttgccg aaggcagcga
     3241 tccaagaccg gcgcctctta cctggagcgc ggcgccaagt accaagggcg tttcgctggc
     3301 ggtgcataac aacggcacca cacatgtaaa gctcctcgat ctggttctca cgacgtcgga
     3361 aggcacgcac atcccgattg cgcccggcac aatgtcctat gtgcttgcag gcggttcgca
     3421 ccattgggag agcgcgattc ccaacgtcaa ggccggagat agactgcata tcaccggaca
     3481 tgaggacagc cggggcacga tgatcgatgc gccgatcatc gtcactccgt gaccaacgtc
     3541 catgttggtg ttctggctct cgcggcggcg ttgagctgga cgtggtgcgc ggcggccttc
     3601 gccggcgata ccc
//
LOCUS       DASWPU010000546         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_109133,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000546 DASWPU010000000
VERSION     DASWPU010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109133"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VGC27_11480"
     CDS             complement(<1..386)
                     /locus_tag="VGC27_11480"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0283231.1"
                     /translation="MAVPHDTTETDAPPWVRVSQDARKLRITAGGAWIARHAALLDPQ
                     LREIGAGGAADVEIDGSGIASLDSTGCWLLVRTKIGFEAAGQRVPLFAVPKLYAALLA
                     KIEAEHVVPPVEILERNTLTSFLERIG"
     gene            complement(485..>1837)
                     /locus_tag="VGC27_11485"
     CDS             complement(485..>1837)
                     /locus_tag="VGC27_11485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0283232.1"
                     /translation="AYTAPQVAGAQKELSKEWWKSFASSELTSFTETAMADNLDLAIA
                     VARMQQAQAQTGLTASALFPAVGLSGDVSRQGSRTPGTTFNTFGVGLGASYELDFWGL
                     AQNNLRAARNSARAAIYAREVVELSTSANVANTYFAVLSLRERLAISRKNIEAAKRIL
                     AITQAKVTNGVSSNLELAQQTAILAGVEAQIPALEQQEREARYSLAILLGRVPEGFDV
                     SADSLEGITAPPVEPGIPSTLLERRPDIAQAEAQLFAAHANLDAARAAFLPSISLNGS
                     GGYNGAEISNLINPSNLAWNIGASLMQTIFDGGRLTSQRDFAKAQEAELVAVYRGTVL
                     NALADVETALGSESSLAERERLTQEQVDNAAEAFRISELQYREGVVDLLTVLQAQQTL
                     FSSQDTLVQVKLARLQAGVGLYRALGGGWTVAAEADRSTRNVFLPFPDITDLPVPNPF
                     "
BASE COUNT          301 a          595 c          621 g          320 t
ORIGIN      
        1 ccgatgcgtt ccaggaacga tgtcagcgtg ttgcgttcca ggatttcaac cggcggcacc
       61 acatgctcgg cctcgatctt ggcaagcagc gccgcataaa gcttcggcac cgcgaacagc
      121 ggcacgcgtt ggcccgccgc ctcgaagccg atcttggtgc gcacgagaag ccagcagccg
      181 gtgctgtcca ggctggcaat tccgctgccg tcgatctcga cgtccgccgc gccgccggcg
      241 ccgatctcgc gcaattgcgg gtcgagcagg gcggcatgcc gcgcgatcca cgctccgccc
      301 gccgttatgc gcagcttgcg cgcgtcctgc gaaaccctga cccagggggg cgcgtcggtt
      361 tcggtcgtgt cgtggggtac cgccattccg gctcatctta gccgggtagg ggctataaga
      421 gcaaagcgca ccccccgcgc cgccgattcg ccggcggcgg gccgactgcg cccggcagag
      481 cggttcagaa cgggttcggg accggcaggt cggtgatatc cgggaacggc aagaagacgt
      541 tgcgcgtgga cctgtccgcc tccgccgcga ccgtccagcc gccgcccagc gcgcgataaa
      601 ggccgacgcc tgcctgcagg cgcgccagct tgacctgaac cagcgtgtcc tgggacgaga
      661 acagcgtctg ctgggcttgc agcaccgtca gcagatcgac gacgccttcg cggtattgca
      721 gttcggagat gcggaaggct tcggcggcgt tatccacctg ttcctgcgtc aaccgctcgc
      781 gctcggccag gctggactcg ctgcccagag ccgtctcgac gtcggcgagc gcattcagca
      841 ccgtgccgcg atagacggcg accagctcgg cttcctgcgc cttggcgaaa tccctttgcg
      901 aggtcagcct gccgccgtcg aagatcgtct gcatcagcga agcgccgata ttccaggcca
      961 gattgctggg gttgatcaga ttggagatct cggcgccgtt atatccgccg ctgccgttca
     1021 ggctgatcga aggcaggaag gcggcgcgcg cggcatcgag attggcatgc gcggcgaaca
     1081 attgggcttc ggcttgtgcg atgtcggggc ggcgctccaa caaggtcgaa ggaatgccgg
     1141 gctcgaccgg cggcgccgtg atgccttcca gcgaatccgc ggacacgtcg aagccttccg
     1201 gcacgcggcc gagcaaaatc gccagcgaat agcgggcctc gcgctcctgt tgttccagcg
     1261 ccggaatctg cgcctctacg cccgccaaaa tcgccgtctg ctgggcgagt tccaggttgg
     1321 aggaaacgcc gttggtgacc ttggcctggg tgatcgccag gatgcgcttg gcggcttcga
     1381 tgttcttcct ggagatggcg aggcgctcgc gcagcgacag cacggcgaaa taggtgttgg
     1441 cgacgttggc gctggtggac agttctacga cttcccgggc gtagatggcg gcgcgcgccg
     1501 aattgcgcgc ggcgcgcaga ttattctgcg ccaggcccca gaaatcgagt tcataactgg
     1561 cgcccagccc gaccccgaag gtattgaaag tcgtgccggg ggtcctggaa ccctggcgac
     1621 tgacatcgcc gctcagaccg acggcgggga agagtgcgga ggcggtcaga cccgtctggg
     1681 cctgggcctg ctgcatgcgc gcgaccgcga tcgccaggtc gagattgtcg gccatcgccg
     1741 tttctgtgaa gctggtcagc tcggaactgg caaaactctt ccaccactcc ttggaaagct
     1801 ctttctgggc gcccgccact tgaggggcgg tgtaggc
//
LOCUS       DASWPU010000547         6462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_4407, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000547 DASWPU010000000
VERSION     DASWPU010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4407"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..212)
                     /locus_tag="VGC27_11490"
     CDS             complement(36..212)
                     /locus_tag="VGC27_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283233.1"
                     /translation="MVLRLIIRTLFLIAALAALKARAIGIDFAGQQVDALPHDVHDWR
                     LDAILTEQGLRRFA"
     gene            284..1096
                     /locus_tag="VGC27_11495"
     CDS             284..1096
                     /locus_tag="VGC27_11495"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEY0283234.1"
                     /translation="MHILFIGDIIGRPGREVVAAELPRLKDSLKLDFVIANGENAAGG
                     FGLTRMVADELFAAGIDCLTTGNHWADQKEILSFIDHEDRILRPRNYPPGTPGKGAGL
                     YETRSGAHVLVTNVMGRVFMDALDDPFAALAAELTACPLGEGADAVVVDIHAEATSEK
                     MAIGHFCDGRASLVVGTHSHIPTADAQILPGGTAYLTDAGACADYDSVIGMEKFEPVQ
                     RFIKKMHGSRFTPATGPATFCAVFVATDAKGFATRIDPVRVGGRLQQALPAL"
     gene            1140..1442
                     /locus_tag="VGC27_11500"
     CDS             1140..1442
                     /locus_tag="VGC27_11500"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0283235.1"
                     /translation="MSITLEQTLGILRANTDLLRAKGVRHAAVFGSVARGGSRDDSDV
                     DVLVEVDTAKHIGLFGYVAIQLALTELIGRKVDVADATTLKPIVREEIMREKVDAF"
     gene            1432..1809
                     /locus_tag="VGC27_11505"
     CDS             1432..1809
                     /locus_tag="VGC27_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEY0283236.1"
                     /translation="MPSDEDRVARRLLDMIENIDKAAGFVAGYTYDRFVDDDRTVYAV
                     VRALEIVSEASRHVPEDIKKRHAAIEWQAIGAAGNVYRHGYHGVNTEVVWDTVVRHLG
                     ALRTAAVTEVERLGQSGHLPKSE"
     gene            1908..2933
                     /locus_tag="VGC27_11510"
     CDS             1908..2933
                     /locus_tag="VGC27_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0283237.1"
                     /translation="MEYRQLGRSGFKVPALTLGTATFGGKGELFKAWGDTDVKGAARL
                     VDICLDAGLTMFDSADVYSSGEAEEVLGAAIKGRRDKVLISTKATFRLGDGPNDAGSS
                     RFHLRQAVEGSLKRLGTDHIDLFQLHGFDALTPVEEVLATLDGFVREGKILYLGVSNF
                     SGWHLMKSLAAADRYGFSRYVAHQIYYSLIGRDYEWELMPLGLDQGVGAVVWSPLGWG
                     RLTGKIRRGQPLPKVSRLHETAKYGPQVSEEHLYRVVDALDEVAKETGKTIPQIALNW
                     LLQRPTVSTVVIGARTEEQLRDNLGAVGWSLTKEQVAKLDAASAVTPAYPYWHQSDFP
                     ERNPPPL"
     gene            complement(2976..3533)
                     /locus_tag="VGC27_11515"
     CDS             complement(2976..3533)
                     /locus_tag="VGC27_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0283238.1"
                     /translation="MSIESLKERLPEYAKDLKLNLSSLANEASLNEQQRAGTFIACAL
                     ASRNQTTTKAIVAEFAPRLSPEANSAARAAAAIMGMNNIYYRFAHLAEAPDYKTMPAK
                     LRMNVIGKPGVDKADFELWCLAVSAINGCGACIDSHEKVLRNAGVTAEQVQAAVRIAA
                     VVHAVAATLDGEEHTADVAAITLAA"
     gene            complement(3752..4294)
                     /locus_tag="VGC27_11520"
     CDS             complement(3752..4294)
                     /locus_tag="VGC27_11520"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0283239.1"
                     /translation="MLSVGDKFPEFSLTGVVSTDPKTAFADFTQASDAGKWKLVFFWP
                     KDFTFVCPTEIAGFGKLYDDFKDRDTVIYGVSVDSEFVHLAWRQNHADLKNLPFTMLS
                     DIKRELSSALGVLDVNAGVAKRASFLVDPEGVIRFVYVTDMSVGRNPQEVLRVLDALQ
                     TDELCPCNWQKGDDVLRPAA"
     gene            4456..5220
                     /locus_tag="VGC27_11525"
     CDS             4456..5220
                     /locus_tag="VGC27_11525"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY0283240.1"
                     /translation="MPPAVFVSHGSPTLPFDDCPARDFLRSLGGRLGRPKAVLVASAH
                     WDTPAPALNAVPRNATIHDFYGFPKPLYELRYEAPGAPAFAERAASLLSGAGLNASID
                     SERGLDHGAWVPLMLMYPGADIPVFQVSVQSGAGAAHHLALGRALAPLRADGVLILGS
                     GGFVHNLSTIDWNGGPEPEWSRAFAEWTHQSLLARDETALADYRLRAPRAAQAHPTEE
                     HFMPLFVAYGAGGESVERLHSSATFGSLRMDAYSFG"
     gene            complement(5254..5610)
                     /gene="queD"
                     /locus_tag="VGC27_11530"
     CDS             complement(5254..5610)
                     /gene="queD"
                     /locus_tag="VGC27_11530"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEY0283241.1"
                     /translation="MKIVQAFHFEAAHSLPNVPPGHRCRRLHGHSYRVELKLEGPVDP
                     ATGFVADFFDIEKAFAPVLARLDHRHLNTVEGLANPTAENIAVWIWQATKPALPQLCA
                     VTVHETKDCRAEYEGE"
     gene            5808..6128
                     /locus_tag="VGC27_11535"
     CDS             5808..6128
                     /locus_tag="VGC27_11535"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0283242.1"
                     /translation="MGESRPERLLEFPKGAQDKAATALTFVAEGAKPDIAKPLAGLGS
                     GVWELAIKSRGDAYRVVYALQLGDDIWVVHAFRKKSKSSIATPKPEIDLVKARIKRLK
                     EQMR"
     gene            6176..6460
                     /locus_tag="VGC27_11540"
     CDS             6176..6460
                     /locus_tag="VGC27_11540"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0283243.1"
                     /translation="MFRDLGRPDAELQQLKALLAAEIVRCLDERGLTVRKAEGLTRIA
                     AADFSRLRNADLGRFTVDRLMTILGRLGRKVEVAVTVRRKRVAEIHPASA"
BASE COUNT         1183 a         1949 c         2096 g         1234 t
ORIGIN      
        1 cgccctgccg atgatatcgc cgagaaaaag aatgttcatg cgaaacgcct gaggccttgc
       61 tcggtcagga tggcatccag ccgccagtca tgcacgtcat gcggcagcgc gtccacctgc
      121 tggccggcga agtcgatgcc gatggcgcgc gctttcagcg ccgccagagc cgcgatgagg
      181 aagagggtcc ggatgatcag tctgaggacc atgaaggtct ccaccgtgac ggatgcggca
      241 gttttttgat gcgccggaca ccggaaggca atatagcaat tggatgcata tccttttcat
      301 cggcgacatc atagggcggc cggggcgcga ggtggttgcc gccgagctgc cgcgtcttaa
      361 ggattcgctc aagctggatt tcgtcatcgc caacggcgag aacgcggccg gcggcttcgg
      421 cctcacccgc atggtggccg acgagctgtt cgccgccggc atcgactgtc tcaccaccgg
      481 caaccattgg gcggaccaga aggagattct ctccttcatc gatcacgagg accgcatcct
      541 gcggccgcgc aactatccgc ccggcacgcc gggcaaaggc gccggcctct acgagacccg
      601 ttccggcgcg catgtgctgg tgacgaatgt gatgggccgg gtgttcatgg acgcgctcga
      661 cgatccgttc gccgcgctcg cggccgagct gaccgcctgt ccgctgggcg aaggcgccga
      721 tgccgtcgtg gtcgatattc acgccgaggc caccagcgag aaaatggcga tcggccattt
      781 ctgcgacggc cgcgccagtc tggtggtcgg cacccattcg catatcccca ccgccgacgc
      841 gcagattctt ccgggcggca ccgcctacct gaccgatgcg ggcgcctgcg ccgattacga
      901 ttccgtcatc ggcatggaga agttcgagcc ggtgcagcgc ttcatcaaga agatgcacgg
      961 cagccgcttc accccggcga cgggtccggc cacgttctgc gccgtgttcg tggcgacgga
     1021 cgccaaaggc ttcgccaccc gcatcgatcc ggtgcgcgtg ggcggaaggt tgcagcaggc
     1081 gctgcctgcg ctttgagcgg taattccgtc tgccggccgg ttcgtgctat agtgacggca
     1141 tgagcattac gctggaacag accttgggga tattgcgcgc caacaccgat ttgttgcggg
     1201 cgaaaggcgt gcggcatgcc gccgtgttcg gctccgtcgc gcgcggcgga tcgcgcgacg
     1261 acagcgatgt tgacgtgctg gtcgaagtcg acacggcaaa gcatatcggc ctgttcggct
     1321 acgtcgccat ccagcttgcc ttgaccgaac tgattgggcg taaggtggac gtggcggatg
     1381 cgacgacctt gaagcccatc gtcagggaag aaatcatgcg cgagaaggtc gatgccttct
     1441 gacgaggacc gggtcgcgcg ccggctgctc gacatgatcg agaatatcga caaggcggcc
     1501 gggtttgtcg ccggttacac ctatgaccgt tttgtcgatg acgaccgcac ggtctacgcc
     1561 gtggtccggg cgctcgaaat cgtgtcggaa gcttcacggc atgttccgga agatatcaag
     1621 aagcgtcacg ccgccatcga atggcaggcg ataggcgcgg cgggaaacgt gtaccggcat
     1681 ggctatcatg gcgtgaacac ggaggtcgtc tgggacacgg tagtccgtca tctcggggcg
     1741 ttgcgaaccg ccgccgtgac cgaagtcgag cgcttggggc aaagcggcca tctgcccaag
     1801 agcgaataag ggtgaagcgc ccgcgttgcg atcgaagccg ggcgcatggt tgaaatcggg
     1861 tccaagagcc ccaatcttac tctcgatgca gatcgaggaa tgagcgcatg gaatatcgtc
     1921 aattgggccg gtccggcttc aaagttcccg cccttacgct ggggacggcc accttcggcg
     1981 gcaaaggaga actcttcaag gcctggggcg acaccgatgt gaaaggcgcc gcgcgcctgg
     2041 tggacatctg cctcgacgcc gggctgacga tgttcgactc ggccgacgtc tattcgtccg
     2101 gcgaggcgga agaggtgctg ggcgcggcga tcaaaggccg ccgcgacaag gtcctgatct
     2161 cgaccaaggc gaccttccgg ctcggcgacg gccccaatga tgccggctcc tcgcgctttc
     2221 atctgcgtca agcggtggag ggctcgctca agcggctcgg cacagatcat atcgatctgt
     2281 tccagcttca cggtttcgac gcgctgacgc cggtcgagga agtgctcgcc acgctcgacg
     2341 gcttcgtgcg cgagggcaag attctctatc tcggcgtgtc gaatttctcc ggctggcact
     2401 tgatgaaatc gctcgccgcc gccgaccgtt acggtttcag ccgttacgtt gcgcaccaga
     2461 tctattattc gctgatcggg cgcgattacg aatgggagct gatgccgctc ggtctcgacc
     2521 agggcgtcgg cgccgtggtg tggtcgccgc tcggctgggg caggctgacc ggcaagatcc
     2581 gccgcggcca gccgctgccc aaggtcagcc gcctgcacga aaccgccaaa tacggcccgc
     2641 aagtgtccga ggagcatctt tatcgcgtgg tggatgcgct cgacgaagtg gcgaaggaaa
     2701 ccggcaagac gatcccgcag atcgcgctca actggctgtt gcagcggccg acggtttcca
     2761 cggttgtcat cggtgcgcgc accgaggaac agctccgcga caatctcggc gcggtgggct
     2821 ggagcctgac gaaggaacag gtcgccaagc tcgacgcggc cagcgccgtg acgccggcct
     2881 atccctattg gcaccaaagc gacttcccgg agcgcaatcc gccgccgcta tgaattaatt
     2941 ttccaccctc cccttgaggg agggtaaacg accatttatg ccgccagcgt tattgccgcg
     3001 acatccgccg tgtgttcttc gccgtcgagc gtcgcggcga ccgcatgcac cacggcggcg
     3061 atgcgtaccg cggcctgcac ctgttcggcg gtgacgccgg cattgcgcag aactttttcg
     3121 tggctgtcga tgcaggcgcc gcagccgttg atggccgaga ccgccaggca ccacaattcg
     3181 aaatcggcct tgtccacgcc cggcttgccg atgacgttca tgcgcaactt ggccggcatg
     3241 gtcttgtaat cgggcgcctc cgccagatgc gcgaagcggt aatagatgtt gttcatgccc
     3301 atgatcgcgg cggcggcgcg cgcggcgcta ttcgcttccg gcgaaagcct gggggcgaac
     3361 tcggcgacaa tggccttggt cgtggtttgg ttccgcgagg cgagcgcgca ggcgatgaag
     3421 gttccggccc gctgctgttc gttgagcgac gcctcgttcg ccagcgagga taagttcagc
     3481 ttcaggtcct tggcgtattc gggcaggcgt tctttcaggc tttctatcga catggtgtcc
     3541 tcggaagaag tcctcaccct tcgacaggct cagggtgagg gttaactcaa atcccctcat
     3601 gctgagccct catcccgagc ctgtcgaggg acgaagcata agggaaaaag ggaagtcccg
     3661 ccggccgggg gaggggcgag ccggcgggat cgggagcgca atccggcaat agttcgccgg
     3721 aaaattgcgc gaccattaaa atccgcaaaa ctcaggcggc cggcctcaga acgtcgtcgc
     3781 ctttttgcca gttgcagggg cacagttcgt cggtctgcaa ggcatcgagc acgcgcagca
     3841 cttcctgcgg attgcgcccg acgctcatgt ccgtcacgta gacgaagcga atgacgcctt
     3901 cgggatcgac caggaaactg gcgcgcttgg cgacgcccgc gttcacgtcg agcacgccga
     3961 gcgcgctgga aagctcgcgc ttgatgtccg acagcatggt gaagggcagg ttcttcagat
     4021 cggcatggtt ctggcgccaa gccaggtgta cgaattcgct atcgaccgag acgccgtaga
     4081 tcaccgtatc gcgatccttg aagtcgtcgt aaagcttgcc gaagccggcg atctcggtgg
     4141 ggcaaacgaa ggtgaaatcc ttgggccaga agaagacgag cttccacttg cccgcatcgg
     4201 aagcctgggt gaagtcggca aacgcggtct tgggatcggt ggacaccacg cctgtcaggg
     4261 agaattcggg gaatttgtcg ccaacgctca gcacgttcat cactcctatg gaagttctta
     4321 gttgcgagtc attcttagag aaattccaat tttagatcaa ggcaaagctg acgcctgcgg
     4381 caactcgccc taggtatatg gttaacatat tgaattcatg ttgttttcaa agcagtcaag
     4441 caggagatcg ccgccatgcc gcccgccgtc tttgtttccc atggttcgcc gactttgccg
     4501 ttcgacgatt gtccggcgcg ggatttcctg cgcagcctgg gtggcaggct gggtaggccg
     4561 aaagcggttc tcgtcgcttc ggcgcactgg gatacgccgg caccggcgct gaacgcggtt
     4621 ccgcgcaacg ccacgatcca cgatttctac ggctttccga aaccgcttta cgagctgcgt
     4681 tacgaagcgc ccggcgcgcc cgcattcgcg gaacgcgccg cgtcgctgct atccggcgcg
     4741 gggctgaacg cctccatcga ttccgaacgc ggcctcgatc acggcgcttg ggtgccgctg
     4801 atgctgatgt atcccggcgc ggacattcct gtgttccagg tttcggtcca gagcggagcg
     4861 ggtgcggcgc atcacctcgc gctgggacgc gcgctggcgc ccttgcgcgc ggacggcgtg
     4921 ctgatcctgg gttcgggcgg cttcgtgcat aatctcagca ccatcgattg gaacggcggg
     4981 ccggaaccgg aatggtcgcg cgccttcgcg gagtggacgc accaaagcct tctcgcgcgc
     5041 gacgaaaccg cgctcgccga ttaccgcctg cgcgcgccgc gggcggcgca ggcccatccc
     5101 accgaagagc atttcatgcc gctgttcgtg gcttacggcg cgggcggcga gagtgtcgaa
     5161 cgcctgcata gcagcgccac cttcggctcg ctcagaatgg atgcatattc ctttggctga
     5221 tcgcgagcgg gtctttgcgt ccgcgcggcc gccctattcg ccctcgtatt ccgcccggca
     5281 atccttggtt tcgtggacgg tcacggcgca cagttgcggc agcgccggct tggtggcttg
     5341 ccagatccac accgcgatgt tctcggccgt cggattggcc agcccttcca ccgtgttgag
     5401 atgccggtgg tcgaggcgcg ccagcaccgg cgcgaacgcc ttctcgatgt cgaagaaatc
     5461 ggcgacgaag ccggtggcgg gatcgaccgg cccttcgagc ttcagttcca cgcgatagga
     5521 atggccgtgc agccggcggc agcgatggcc cggcgggacg ttgggcaggc tgtgcgccgc
     5581 ctcgaaatgg aaagcctgga cgatcttcat ggtctgcctg tttatccggg gcgagggcat
     5641 atatgcaaac cgccctcagg caaagcttca accggctctg caaagggtac ccaaggtcat
     5701 cctgccgcag agaattcgcg atgtcggcag tccaagattg tacttgcatt tgttgtcata
     5761 tttgactaca ttgaggctcg gatggtccga aacacgcggg cgatcagttg ggtgaaagcc
     5821 gccctgaaag acttcttgag ttcccgaagg gcgctcaaga caaggccgcg accgcgttga
     5881 cctttgtcgc cgagggagca aaacccgata ttgccaagcc gctggccggt ttgggaagcg
     5941 gtgtctggga gttggcgatc aagtctcgcg gcgatgccta tcgggttgtt tatgcgctcc
     6001 agcttggcga cgacatttgg gtggttcatg ccttccggaa gaagtccaag agcagcattg
     6061 cgaccccaaa gcctgagatc gatctggtga aggcgcggat caagcgattg aaagaacaaa
     6121 tgcgatgaga aggctcaagg ccgccaatat cgaaaccgtc aaaggaaccg ggaacgtgtt
     6181 ccgcgatctc ggcaggccgg acgctgagct tcagcagttg aaggcgctgc tcgcggccga
     6241 gattgtgcgt tgcctagacg agcgcggtct caccgttcgc aaagccgagg gtctgacgcg
     6301 aattgcggcg gccgattttt cacgcctccg caatgccgat cttgggcgct tcacggtgga
     6361 tcggttgatg acgatcctgg gccgcttggg ccggaaggtc gaagttgccg tcaccgttcg
     6421 acgcaaaagg gtggctgaga ttcacccggc gtcggcgtag tt
//
LOCUS       DASWPU010000548         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_66574,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000548 DASWPU010000000
VERSION     DASWPU010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66574"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VGC27_11545"
     CDS             <1..298
                     /locus_tag="VGC27_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HEY0283244.1"
                     /translation="EVHDQPQLERALALGADMIGINNRDLTTFATELDVTLRLAPLVP
                     ADKLVVAESGLSRRDDLALLATAGVTTFLIGEGLMRQKDLASATSLLTSAKLFP"
     gene            295..1068
                     /gene="moaC"
                     /locus_tag="VGC27_11550"
     CDS             295..1068
                     /gene="moaC"
                     /locus_tag="VGC27_11550"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY0283245.1"
                     /translation="MTKLSHLDEQGAARMVDVPDKQASARKARAEAVIVLSQAAFAAV
                     ASGGAFKGDVFAAARIAGIMAAKKTAELIPLCHTIPLAQAEIAFEMLAEKSAIRIVAE
                     VKTAARTGVEMEALTAASIAALTVYDMTKAVDKSAVVESIRLLTKSGGKGGPYAPAPQ
                     PAAGKSPPPAPQGRVKPSALAGLITAPAPRPDASGRREAFRAFMVSRRLRATDWAKEA
                     GVPAAQIYAFLTGRLPALPPDAAERLARAAHVRLEDMFR"
     gene            1065..>1840
                     /locus_tag="VGC27_11555"
     CDS             1065..>1840
                     /locus_tag="VGC27_11555"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEY0283246.1"
                     /translation="MISVAEAGKRIAEALSPIDTETMDVAHLAGRVLVEDAVARSNQP
                     PVRVSARDGFAVRLADGGVPRRLIGSAPAGHPFAGKLGAGEAVRIFTGGVVPEGADAI
                     VIQEDAASDGVMVSFDASACSPHFVRAAGLDFKTGDVLVPKGRRMTGRDCALLAAGDI
                     AHAKVRRRPRIAIISIGDELSRPGEERKPGGIVASSGYGLSVMVHDWGGAPCDFGILQ
                     DNEADIATVADADADFIITLGGVSVGDHDLVQKALGTKDFA"
BASE COUNT          286 a          621 c          635 g          298 t
ORIGIN      
        1 cgaagtccac gaccagccgc agttggagcg cgccctggcc ctcggcgcgg acatgatcgg
       61 catcaacaac cgcgatttga caaccttcgc gaccgaactc gacgtgacgc tgcgcttggc
      121 gccgctggtt ccggcggaca aacttgtcgt tgccgaaagc ggcctgtcgc ggcgcgacga
      181 tctggcgctg ctggcgacgg ccggggtcac cacgttcctg atcggcgaag gcctgatgcg
      241 gcaaaaggat ctggcgtcgg ccacgtcctt gctgacgagt gctaagctct ttccatgacc
      301 aagctcagcc atctcgacga acagggcgcg gcgcgcatgg tcgatgttcc ggacaagcag
      361 gcgagcgcac gcaaggcccg tgccgaagcg gtgatcgtgc tgtcgcaagc ggcgttcgcg
      421 gccgtggctt cgggtggcgc gttcaaaggc gacgtgttcg cggcggcgcg catcgccggc
      481 atcatggcgg cgaagaagac ggccgaactg atcccgctgt gccataccat cccgctggcc
      541 caggcggaga tcgccttcga gatgctggcg gagaagagcg ccatccgtat cgtcgccgag
      601 gtgaaaaccg cggcgcggac cggcgtggaa atggaagcgc tgacggcggc gtccattgcg
      661 gcgctgaccg tctacgacat gaccaaagcg gtggacaagt ccgccgtcgt cgaatccatc
      721 cgcctactga cgaagtccgg cggcaagggc gggccttatg cccccgcgcc ccagccggcc
      781 gcagggaaat cccccccgcc cgctccgcag ggccgcgtca agccttccgc gctggcggga
      841 ttgatcactg ctcctgcccc gcgtcccgat gcctccggcc ggcgcgaagc ctttcgcgcc
      901 ttcatggtct cccgccgctt gcgcgccacg gattgggcga aagaagccgg tgttccggcg
      961 gcgcagatct atgcttttct gaccgggcgc ttgcccgccc tgccgcccga cgccgcagag
     1021 cggctcgccc gcgccgcgca tgtgcgcctt gaggatatgt tccgatgatc agcgtcgccg
     1081 aagccggaaa acgcattgcg gaagcccttt cgccgatcga caccgaaacc atggacgtcg
     1141 cccatctggc gggccgcgtt ctggtcgagg acgccgtcgc gcggtcgaac cagccgccgg
     1201 tgcgggtctc ggccagggac gggtttgccg tgcggctggc cgacggcggc gttccgcggc
     1261 gtctgatcgg ctcggcgccg gccggacacc ctttcgcggg caagctcggc gccggagaag
     1321 cggtgcgcat cttcaccggc ggcgtggtgc cggagggcgc ggacgccatc gtcattcagg
     1381 aagacgcggc ctccgacggc gtcatggttt cgttcgacgc ctccgcctgt tccccgcatt
     1441 ttgtccgcgc cgccgggctg gatttcaaga ccggcgacgt gcttgtgccc aaaggcaggc
     1501 ggatgaccgg gcgtgattgc gccctgctcg cggcgggcga catcgcccat gcgaaggtgc
     1561 gccgcaggcc gcgcatcgcc atcatctcga tcggcgacga gttgtcccgg cccggcgagg
     1621 agcgcaaacc gggcggcatc gtggcttcgt cagggtacgg attatcggtg atggtccacg
     1681 actggggcgg cgcgccgtgc gatttcggca tcttgcagga caacgaggcg gacatcgcca
     1741 cggtggcgga cgccgacgcc gacttcatca ttacgctggg cggggtttcg gtcggcgatc
     1801 acgacctcgt tcagaaagcg ctgggcacca aggacttcgc
//
LOCUS       DASWPU010000549         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_28895,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000549 DASWPU010000000
VERSION     DASWPU010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28895"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1648)
                     /gene="pheT"
                     /locus_tag="VGC27_11560"
     CDS             complement(<1..>1648)
                     /gene="pheT"
                     /locus_tag="VGC27_11560"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY0283247.1"
                     /translation="AMKFPLSWLKDHLETDADAATIADKLTSIGLEVEAVEDAGARLK
                     DFVVGYVVSAEKHPNADKLKLCMVDTGGGAPVQVVCGAPNAHTGMKGVFARAGVVIPA
                     SGEVLKVGTIRGVESHGMLCSGRELLLSDDHTGIIELPAGAKVGEPAAKALGLTDAVI
                     DVAITPNRGDCTSVWGIARDLAAAGFGKLKTPKVEAIAGKYPCPKTISLNFTPETKSA
                     CPLFAGRFVRGVKNGPSPKWVQDRLKAIGQRPISALVDVSNLIAHDRGRPLHVFDADK
                     LIGNMQARLAKDGETLLALDGKTYTLDAEMCVIADEKAARGIAGVMGGEETGCSDATV
                     NVFIESATFDPIRVAATGRKLGLQSDARYRFERGVDPEFVVPGLELATQLILDWCGGE
                     PSRIVVAGAVPAWRREIEFAPYTVGRLGGLHVAKDEIVAILTGLGFTVRGREVLQVTP
                     PSWRSDIDGPADLVEEVVRIHGLDHVPSVAMARPDAIARPVLTPAQKRRRLVRRTLAA
                     RGFNETISFSFISRAQALLFGGGDEARQLENPIAADLDALR"
BASE COUNT          289 a          540 c          533 g          286 t
ORIGIN      
        1 gcgcaaagcg tccagatcgg cagcgatggg attttcgagc tgacgcgcct cgtcgccgcc
       61 gccgaacagc aaagcctgcg cacgcgagat aaagctgaag gagatcgttt cgttgaagcc
      121 gcgggcggcc agcgtgcggc gcaccaggcg cctgcgcttc tgggccgggg tcagtacggg
      181 gcgcgcgatc gcgtcgggcc gcgccatcgc caccgacggt acgtgatcca ggccgtggat
      241 gcgcaccact tcttcgacca gatcggcagg tccgtcgatg tcgctgcgcc aggacggcgg
      301 ggtgacttgc agcacctcgc ggccgcgcac cgtaaacccg agtccggtca ggatggcgac
      361 gatctcatcc ttcgccacat gaaggccgcc gagccgtccc accgtgtatg gcgcgaattc
      421 gatctcgcgc cgccaggcgg gaaccgcccc cgccacgacg atccgcgaag gctcgccgcc
      481 gcaccaatcc agaatcaatt gcgtggccag ttccaggcct gggaccacga actcgggatc
      541 gacgccacgc tcgaagcggt agcgcgcgtc cgattgcagt ccaagcttgc ggcccgtcgc
      601 ggcaacacgg atcggatcga aggtggccga ctcgatgaag acattcaccg tggcatcgga
      661 acagccggtc tcttcaccgc ccatgacgcc ggcgatgccg cgcgcggcct tctcgtcggc
      721 gatcacgcac atttcggcat cgagggtgta ggtcttgccg tcgagcgcca gcagtgtttc
      781 gccgtccttc gccagacggg cctgcatgtt gccgatcagt ttgtcggcgt cgaagacgtg
      841 caacggccgg ccgcggtcgt gggcgatcag gttggaaaca tcgaccaggg ccgagatcgg
      901 acgctggccg atggccttca ggcgatcctg cacccatttg ggcgacggac cgtttttcac
      961 tccgcgcacg aagcgcccgg caaatagcgg acaagccgat tttgtctccg gcgtgaagtt
     1021 gagcgagatc gttttcggac aagggtattt gcccgcaatg gcttcgactt tgggtgtttt
     1081 cagttttcca aagccggcgg ccgccagatc gcgcgcgatg ccccaaaccg aggtgcagtc
     1141 gccgcgattg ggggtgatgg cgacgtcgat caccgcgtcg gtgaggccga gcgccttagc
     1201 ggcgggttcg cccaccttgg cgccggcagg caactcgatg atgccggtat gatcgtcgga
     1261 aaggagcagc tcgcggccgg aacacagcat accgtgcgac tccacaccgc gaatggtgcc
     1321 gaccttgagc acttcgccgc tcgccggaat gaccacgccg gcgcgcgcga acacgccctt
     1381 catgccggtg tgcgcattgg gcgcgccgca cacgacctgc accggcgcgc cgcccccggt
     1441 atccaccatg cacagtttca gcttgtcggc attgggatgt ttctcggcgg aaacgacata
     1501 accgacgacg aagtctttga gccgggcacc ggcgtcttcc accgcttcca cttcaaggcc
     1561 gatacttgtc agtttgtcgg cgatggttgc ggcatcggcg tccgtctcca gatggtcctt
     1621 cagccaagag agcgggaact tcatggcg
//
LOCUS       DASWPU010000550         9416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_23563,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000550 DASWPU010000000
VERSION     DASWPU010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23563"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VGC27_11565"
     CDS             complement(<1..1147)
                     /locus_tag="VGC27_11565"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY0283248.1"
                     /translation="MDGFLLSLLLAIQRDVIGRFGGTMTPRSGRKMEPDRVPESRIAT
                     IDKDGLQALLDVLAERGYRVVGPALRDQAIVYDDIDSVRDLPAGWADEQDGGRYRLVR
                     RSDNALFGYVVGPHSWKKYLYPPIRRLWSATRTAGAVKVTPEPLPSERFAFIGVRACE
                     LHAIAIQDHVFLDGIVPDAHYRARRENAFFVAVNCGTAGGTCFCVSMNTGPKADGGFD
                     LALTELIDGETHRFTVEAGSDKGREVLAALPHRAAKQEDVAAADAAVAKAAAAMGRSM
                     RTDDIHDLLMGNLEHPRWDDVAERCMTCGNCTMVCPTCFCMTVEDSSDLAGVESSRSR
                     RWDSCFTLDHTYVHGGSVRTSAKSRYRQWLTHKLATWIDQFGSSGCVG"
     gene            complement(1216..3378)
                     /gene="malQ"
                     /locus_tag="VGC27_11570"
     CDS             complement(1216..3378)
                     /gene="malQ"
                     /locus_tag="VGC27_11570"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY0283249.1"
                     /translation="MDADEALRRLAQAAGIEARYWDIRGGLHETAPATMRALLGALGF
                     AADTDGDVATGLASIAQEPWRTPLPSVVVARENCELDVPVRLPEAAGRTIRWTVRLET
                     GEVRTGECGLDTLPIEEMGRFGETQIARRRLKLAPLPAGYHDLHLAASGEARARLIVA
                     PQRCYLPPKFSAAKSWGLAAQLYTLKSSNDWGIGDFGHLRTLIERLAEGGADAVGLNP
                     LHALFLDAPDEASPYSPSSRLFRNPLYLEVTAIPDFAESEEARALIESPDVARLLRRA
                     RGAAFVDYKAVAEVKLAVLQCLYDSFAANHVSRMDARAAAFLGFVEHAGRALDRFATF
                     QMLSEHFGTHAWLRWPAACRDPESGLVSELRGRTAERVSFFKYLQWQCEEQLAAAAEL
                     ARRGGMDVGLYNDLAVSVDEASADHWSRQDLFIGGVSVGAPPDPFNEAGQGWGVIPLN
                     PFRLRAAAYEPFVALLRANMRHAGALRIDHVMGWQRLFLIPHGAKPAAGAYVNYPLDD
                     LLAIAALESQRHRCAVIGEDLGTVSAGFRERMAAANVLSCRVFAFERDGGRFRRPHEY
                     PALASVSAATHDLATLAGFWGGDDIAAKAKLGLFKSPEEEANARSGRSADKRLLLQAL
                     ADEALLPDGVSPPDADRLGWTPELALAVHAYLARSPSLLFLAQLDDLAGVAHQANLPG
                     STTQYPNWRRRQARTLDELFADPATQKEMTVIAAERAK"
     gene            complement(3393..5507)
                     /gene="glgX"
                     /locus_tag="VGC27_11575"
     CDS             complement(3393..5507)
                     /gene="glgX"
                     /locus_tag="VGC27_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY0283250.1"
                     /translation="MGLEFRLKQGWPFPLGATFDGQGVNFALFSAHAKKVKLCLFEDG
                     GRREIARLTLPECTDDVWHGYLPAGRPGLLYGYRVHGPYDPFEGHRFNPNKLLIDPYA
                     RALSGPLDWNEIHCGYTVGDPRGDLSFDGRDNAALMPKCRVVGPVPAWDDDTIPHTPR
                     SHSVIYELHVRGYTMRHPAVGEAVRGTMAGLRHPEVIRHLRELGITAVELLPVQPFCT
                     SQVLAKNGLSEFWGYNSVNFFAVEPRYLGGDSEDFRATVRAFHDAGIEVILDMVFNHS
                     GEGDELGPTFSFRGIDNASYYCLAGDRRRYRDTTGCGNTLNVGHPRVQQMVMDSLRYW
                     ATEMHVDGFRFDLAVSLVRSEHDFSPAAALFACILQEPALARTKLIAEPWDLGPGGYR
                     LGAFPSRWSEWNDRFRDDVRRFWRGDEGRVGDLAFRLSGSSDVFDLSKRKPTAGVNFI
                     TAHDGFTLADLVSYSVKHNEANKENGLDGSDNNFNWNCGAEGPTGDPAIIRLRERQKR
                     NLMATLLLSLGIPMIVAGDEFGRSQRGNNNAYCQDNEIGWVDWTELERNREFLIFVRR
                     LIKLRADHPVFRRSSFFLGDRTEESDIKDIVWLSPAGREMTHADWSAWQCRCLGVRYA
                     ATSEMDTETYTRRLDPHAFVLLMNAGPQSVTFVLPDTGADRKWSRLIDTAAADDAPEA
                     HFEPQSAFRLGAHALALFVSEI"
     gene            complement(5510..7732)
                     /gene="glgB"
                     /locus_tag="VGC27_11580"
     CDS             complement(5510..7732)
                     /gene="glgB"
                     /locus_tag="VGC27_11580"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY0283251.1"
                     /translation="MRPLTQQRMVVGPDTPMDRIAALLAGDEGNPFSFLGMHEDSASG
                     RLVVRAFQPGATRVAVVSRETGAETAELTCTHDAGFFAGAVATGSGRFPYRLRVWYGA
                     TDIVIDDPYSFGPMLGELDLYLHAEGNYFRAYEKMGAHPAVMDGVSGVNFAVWAPNAR
                     RVSVVGDFNFWDGRRNPMRLHPGAGIWEIFLPGVAPGARYKFEIKTRGGALFLKADPY
                     AFEAERPPQTASVVCATPPTPRQMEEAGPRADRHAPVSIYEVHLGSWRRVPEEGERPL
                     TYRELADRLVPYVKDMGFTHLELMPVNEHPFAGSWGYQPTALFAPTSRFGTPDEFRDF
                     VKRCHAEGVGVILDWAAGHFPGDAHGLAQFDGTHLYEHADPREGYQPDWSSYIYNFGR
                     NEVRGFLLSNALFWLSQYGVDALRVDAVASMLYRNYSRKEGEWIPNVFGGVENLEAVD
                     FLRRLNELVYANRPKAVMVAEESTAWPMVSRPTYLGGLGFGYKWNLGWMHDTLDYMAK
                     EPIHRKYHHDRLTFGLLYAFNENFILPLSHDEVVHGKGSLFSKMPGDRWQKFANLRAY
                     YAFQYTQPGKKLLFMGGEFAQEREWDHDTSLDWHLTEDPLHAGVQKLVRDLNRLYRGR
                     KALHERDCEGEGFSWIDCNDNESSVISYLRKAADPNDFVAVVCNFTPVVRHGYRVGVP
                     RAGRYREVLNTDSTHYGGSDVGNAQDVWTEDHPSHGMAASLSLTLPPLGVLVLAPEGM
                     "
     gene            7965..>9416
                     /gene="glgA"
                     /locus_tag="VGC27_11585"
     CDS             7965..>9416
                     /gene="glgA"
                     /locus_tag="VGC27_11585"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEY0283252.1"
                     /translation="MRVLFVTAEAYPLAKTGGLADVSRALPIALARSGIDIRLLLPGY
                     PRAYTRLERPRIVAKLEPTLGITDATLIAGTFPGTDLPVWLIDSRTLFRRNGGLYQDE
                     NGRDWPDNALRFAFLAHVGAQLAMGRTKVAWKPDIVHANDWHTGLLPLILAHEKGPRP
                     SCVFTSHNMAFQGNFPAEVLPSLNIPESCFTTNGMEFYGQVSFLKAGLSYADRVTTVS
                     PTYAMEIVTPEFGCGMDGILRARGSNFSGILNGIDDALWDPATDPHLPQTYRAMDISG
                     KRVCKAALQNELGLPASPDTPLIGFVSRLTQQKMADVILEALPWIAEQKAQLALVGEG
                     DPSLEEAFTAAQVRYGENVAVNIGYDEPLAHRLQAGCDILLAPARFEPCGLTQLYALR
                     YGSLPVVRRTGGLADTVVDATAETIANRTATGFVFEGADMPSLISATGRALAFYREPL
                     TWRRLQLHAMAQDFSWNASAAKYALLYSEASGVP"
BASE COUNT         1777 a         3076 c         2956 g         1607 t
ORIGIN      
        1 agccgacgca gcccgacgag ccgaactgat cgatccaggt cgcgagcttg tgggtaagcc
       61 attggcggta gcgcgatttc gcgctcgtcc gcacgctgcc gccgtgaaca taggtgtgat
      121 ccagggtgaa gcaggaatcc cagcgccgcg aacgcgagct ttccaccccc gccagatcgc
      181 tggaatcttc caccgtcatg cagaagcagg tcgggcacac catcgtgcag ttgccgcagg
      241 tcatgcagcg ctcagccacg tcgtcccagc gcggatgctc gagattgccc atgagcagat
      301 cgtgtatgtc gtcggtccgc atcgaccggc ccatcgccgc cgccgccttg gcgacagcgg
      361 catcggccgc cgctacgtct tcctgcttcg cggcccgatg aggcaaagcg gccagcacct
      421 cccggccctt gtccgacccc gcttcgacgg taaaacgatg cgtctcgccg tcgatgagtt
      481 cggtcagagc aagatcgaac ccgccgtccg ccttcggccc cgtgttcatc gagacgcaga
      541 agcaggtgcc gccggcggtg ccgcaattga cggcgacgaa aaaggcgttc tcgcggcgcg
      601 cccggtaatg cgcatcgggc acgatgccgt cgaggaagac atgatcttga atcgcgatgg
      661 cgtgcagctc gcaggctcgc acgccgatga aggcaaagcg ttcgctggga aggggctcgg
      721 gcgtcacctt cacggcgccg gccgtgcgcg ttgcgctcca cagtctgcgg atcggcggat
      781 agagatattt cttccaggaa tgcgggccca ccacatagcc gaacagggcg ttgtcggaac
      841 gccggaccag gcggtagcgc ccgccgtcct gctcgtccgc ccagccggcg ggcaaatccc
      901 gcaccgaatc gatatcgtca tagacgatcg cctggtcgcg cagcgccggc cccacgacgc
      961 gatagccgcg ttcggccaga acatcgagaa gggcctgaag cccgtccttg tctatggtgg
     1021 caatccggct ttctgggacg cgatcaggtt ccattttccg gccgcttctc ggcgtcatcg
     1081 ttccgccgaa gcggccgatg acgtcgcgct gtatggcgag caatagacta aggagaaatc
     1141 cgtccacgcg caatgccgcg tgcgtccttg agcggcgttg aagatcgcga cggaaaaagt
     1201 aatatgtaaa gtatgttatt tcgcgcgttc ggccgcgatc accgtcattt ctttttgcgt
     1261 ggccggatcg gcgaacagct cgtcgagcgt ccgcgcctgg cgccggcgcc agttcggata
     1321 ttgcgtcgtg ctgccgggaa gattcgcctg atgcgccacg ccggccagat cgtccagctg
     1381 cgcgaggaac aacaggctcg gcgaccgcgc gagataggcg tgtaccgcca aggcaagttc
     1441 cggcgtccag ccgaggcgat ccgcatccgg cggcgaaacg ccgtccggca acagcgcctc
     1501 gtccgcaaga gcttgcagca gcaggcgctt gtcggccgaa cggccggagc gggcgtttgc
     1561 ttcctcttcg ggggatttga acaatccgag cttcgctttc gctgcgatgt cgtcaccgcc
     1621 ccaaaacccg gcgagggtcg ccagatcgtg agtggcggcg gacaccgacg ccagcgccgg
     1681 atactcgtgc ggccgccgga accggccgcc gtcgcgctcg aaggcgaaga cgcggcagga
     1741 cagaacattg gccgccgcca tgcgctcgcg gaagccggcc gagaccgtgc ccaaatcctc
     1801 gccgatgacg gcacagcgat ggcgctggct ctccagagcc gcaatcgcca gcagatcgtc
     1861 gagcggataa ttgacataag cgcccgctgc gggcttggcc ccatggggaa tcaaaaacag
     1921 gcgctgccag cccatcacat gatcgatccg cagcgcgccg gcatggcgca tattggcccg
     1981 cagcaaggcg acgaagggtt cgtaagcggc ggcgcgcaag cggaagggat tgagcggaat
     2041 gacgccccag ccctgccccg cttcgttgaa cggatcgggc ggtgcgccga cgctgacgcc
     2101 gccgatgaac aaatcctggc gggaccaatg gtcggcgctc gcctcatcca cgctgaccgc
     2161 cagatcattg tagagcccga cgtccatacc gccgcggcgc gccagctcgg cggccgcggc
     2221 caattgttct tcgcattgcc attgcaggta tttgaagaac gagacacgct cggcggtgcg
     2281 cccgcgcagt tccgaaacca gaccggattc cggatcgcgg caagcggcgg gccagcgcag
     2341 ccaagcgtgg gttccgaaat gctcgctcaa catctgaaag gtcgcaaagc gatcgagcgc
     2401 ccggccggcg tgctccacaa agcccagaaa cgccgccgcg cgcgcgtcca tgcgcgagac
     2461 atggttcgcg gcgaaactat cgtagaggca ttgcagaacc gccagtttga cctcggcgac
     2521 ggctttgtaa tcgacgaagg cggcgccgcg cgcacgccga agcaaacgcg cgacatcggg
     2581 ggactcgatg agcgcgcgcg cctcctcgga ttcggcgaaa tcgggtatcg ccgtcacctc
     2641 cagatagagc ggattgcgga acaagcggct actcggcgaa taggggctcg cctcgtctgg
     2701 cgcatccagg aacagcgcgt gcagcggatt gaggcccacc gcgtccgccc cgccctcggc
     2761 cagccgctcg atcagcgtcc gtagatggcc gaagtccccg atgccccaat cgttggacga
     2821 cttgagtgtg taaagctgcg ccgccaagcc ccagcttttc gcggcggaga atttaggcgg
     2881 cagatagcag cgctggggcg ccacgatcag acgcgcgcgc gcttcaccgg aggcggcgag
     2941 atgaagatcg tggtagccgg cgggaagcgg cgccagcttg agccgccgcc gcgcgatttg
     3001 cgtttccccg aagcgcccca tttcctcgat cggcaaagta tcgaggccgc attcgccggt
     3061 gcgcacctcg ccggtctcca agcgaacggt ccagcggata gtgcggccgg ccgcttccgg
     3121 cagacggacc ggcacgtcga gttcgcaatt ttcccgcgcc accacgaccg acggcaatgg
     3181 agtgcgccac ggctcctgcg cgatggaagc caacccggtg gcgacatccc catccgtgtc
     3241 cgcggcaaac cccagcgcgc caagcagcgc gcgcatcgta gcgggcgcgg tttcatgcag
     3301 cccgccgcga atgtcccagt agcgcgcctc gatgcccgcc gcctgcgcca gccgtctcag
     3361 agcttcgtct gcatccatgg agcggccggt ctctagattt cgctgacaaa cagggcaagc
     3421 gcgtgcgccc ccaggcggaa ggcggattga ggctcgaaat gtgcttccgg cgcgtcgtcg
     3481 gcagcggccg tatcgatcag gcggctccat ttgcggtccg ccccggtatc gggaagcacg
     3541 aaggtcacgc tttgcggccc ggcattcatc aacagaacaa aggcgtgagg atccaagcgc
     3601 cgcgtatagg tctcggtgtc catttcggag gtcgcggcat aacgcacgcc caggcagcgg
     3661 cattgccaag cgctccaatc ggcgtgcgtc atttcccgcc cggcaggcga cagccagacg
     3721 atgtccttga tgtcgctttc ctcggtgcgg tcgcccagaa agaaagacga gcggcggaaa
     3781 accggatgat ccgcgcggag ttttatcagg cgcctcacga agatcaggaa ttcccgattg
     3841 cgctccagtt ccgtccagtc cacccagccg atctcgttgt cctggcaata ggcgttgttg
     3901 ttgccgcgct ggctgcggcc gaattcgtcg cccgcgacga tcatcggaat gccgagcgac
     3961 aacaacagcg tggccatcag gttgcgcttc tgccgttcgc ggagccttat gatcgcggga
     4021 tcgccggtcg gaccttccgc gccgcagttc caattgaaat tgttatccga cccgtcgagg
     4081 ccgttttcct tgttggcctc gttgtgcttg acgctgtagc tcaccaggtc ggcaagcgtg
     4141 aagccgtcat gcgcggtgat gaaattcact ccggccgtgg gtttgcgctt gctcagatcg
     4201 aacacatcgc tcgaaccgga gagccggaag gccagatcgc cgacccggcc ttcgtcgccg
     4261 cgccagaacc ggcgcacatc gtcgcggaac ctgtcgttcc attcgctcca gcgcgacgga
     4321 aaggccccga gccggtatcc gccggggccc agatcccaag gctccgcgat gagcttggtg
     4381 cgggcgagcg ccggttcctg aaggatgcag gcgaacagcg ccgccgccgg cgagaaatca
     4441 tgttccgacc gcaccaggct gacggcaagg tcgaagcgga agccgtccac atgcatctcc
     4501 gtggcccagt agcgcagcga atccatcacc atctgttgaa ctcgcggatg gccgacgttc
     4561 agcgtgttgc cgcagccggt ggtgtcgcgg tagcgccgcc ggtcgccggc gaggcaatag
     4621 taggaggcgt tgtcgatgcc gcggaacgag aaggtcgggc ccaattcgtc gccctcgccg
     4681 ctgtggttga acaccatgtc gagaatgact tcgatgccgg cgtcatggaa cgccctgacg
     4741 gtggcgcgga agtcttcgct gtcgccgccg agatagcgcg gctcgaccgc aaagaaattg
     4801 accgaattgt agccccagaa ttcgctgagc ccgttcttcg ccagcacttg cgaggtgcag
     4861 aaaggctgca ccggcaagag ttcgaccgcc gtgatcccaa gctcgcgtaa atggcggatg
     4921 acctcgggat ggcgcagccc cgccatggtt ccgcgcaccg cctcgccgac cgccggatgg
     4981 cgcatggtgt agccgcgcac gtgcaattcg tagatcacgc tgtgcgagcg cggcgtatgg
     5041 gggatggtgt cgtcgtccca ggcggggacc ggcccgacga cgcggcactt gggcatcagg
     5101 gcggcattgt cgcggccgtc gaaggaaaga tcgccgcgcg gatcgccgac ggtgtagccg
     5161 caatgaatct cgttccagtc cagcggcccg ctgagcgcac gcgcataagg atcgatcaga
     5221 agcttgttgg gattgaagcg atggccctcg aagggatcgt acggcccgtg gacgcggtag
     5281 ccgtaaagca gccccggcct gccggccggc agatagccgt gccagacatc gtcggtgcat
     5341 tcgggcagcg tcagccgcgc gatctcgcgc cgcccgccgt cctcgaaaag gcaaagtttc
     5401 accttcttcg catgggccga gaacaacgcg aagttgacgc cctgcccgtc gaacgtggcg
     5461 cccagcggaa aaggccaccc ctgcttgagc cggaattcca atcccacggc tacattccct
     5521 cgggcgccag caccaggacg ccgagcggcg gcagcgtcag cgacaaggac gcggccatgc
     5581 cgtgggaggg atggtcttcg gtccatacat cctgcgcgtt gccgacgtcg ctgccgccgt
     5641 aatgggtcga atccgtattg aggacttcgc ggtacctgcc cgcgcgcggc acgccgaccc
     5701 ggtagccatg gcgcacgacg ggcgtgaaat tgcacaccac ggccacgaaa tcgttggggt
     5761 ccgccgcctt gcgcaaatag gaaatcacgc tggactcgtt gtcgttgcag tcgatccagg
     5821 aaaacccctc gccttcgcaa tcgcgctcgt gcagcgcctt tctgccgcga taaagccggt
     5881 tcaaatcgcg gaccagcttc tgcacgcccg cgtgcagggg atcttcggtc aagtgccagt
     5941 ccaggctggt gtcgtgatcc cattcgcgct cctgcgcgaa ctccccgccc atgaacaaca
     6001 gcttcttgcc gggctgggtg tactggaagg cgtaataggc gcgcagattg gcgaatttct
     6061 gccagcggtc ccccggcatc ttgctgaaca gggaaccctt gccgtgaacg acttcgtcgt
     6121 gcgacagggg cagaatgaaa ttctcgttga aggcgtaaag caggccgaag gtcagccggt
     6181 cgtggtgata tttgcgatgg atcggctcct ttgccatgta atccagcgtg tcgtgcatcc
     6241 agccgagatt ccatttgtag ccgaagccca agccgccgag ataggtcggc cgcgagacca
     6301 tcggccacgc cgtcgattct tccgccacca tgaccgcctt cggccggttg gcataaacga
     6361 gttcgttcaa gcggcgcaga aaatcgacgg cctccagatt ctcgacgccg ccgaagacgt
     6421 tgggaatcca ctcgccttcc ttgcggctgt aattgcggta cagcatcgag gcgacggcat
     6481 cgacgcgcag cgcatcgacg ccgtattgcg acagccagaa cagcgcattg gagagcaaaa
     6541 agccgcgcac ctcgttgcgg ccgaaattgt agatgtagct gctccaatcg ggttggtagc
     6601 cctcacgcgg atcggcatgt tcgtaaaggt gggttccgtc gaactgcgcc aggccgtgcg
     6661 cgtcgccggg gaaatggccg gcggcccagt ccaggatcac gcccacccct tccgcatgac
     6721 agcgcttgac gaaatcgcgg aactcgtccg gcgtgccaaa gcggctggtc ggcgcaaaca
     6781 gggccgtcgg ctggtagccc caggacccgg cgaacggatg ctcgttgacc ggcatcaact
     6841 ccagatgggt gaaccccata tccttgacat agggaaccag ccggtcggca agctcgcgat
     6901 aggtcagcgg gcgctctccc tcttcaggca cgcgccgcca cgaaccgaga tgaacctcgt
     6961 aaatcgaaac cggggcatgg cggtcggcgc gagggccggc ttcctccatc tgccttggcg
     7021 tgggcggcgt ggcgcaaacc accgatgcgg tctgcggcgg acgttcggct tcgaaggcgt
     7081 atgggtcggc tttgaggaac agcgcgccgc cccgggtctt gatttcgaat ttgtagcgcg
     7141 cgccgggagc cacgccggga aggaatattt cccaaatccc ggcgccggga tgcagccgca
     7201 tcgggttgcg gcgcccgtcc cagaagttga aatcgcccac cacgctgacg cgccgcgcat
     7261 tgggagccca gacggcgaaa ttcacccccg acacgccgtc cataacggcc ggatgcgcgc
     7321 ccatcttctc ataggcgcga aaataattgc cttcggcgtg gagatagagg tccagttcgc
     7381 caagcatcgg gccgaacgag tagggatcgt cgatcacgat atcggttgcg ccgtaccaca
     7441 cccgcaacct gtagggaaac cggccactac ccgttgcgac ggcgccggcg aagaaccctg
     7501 catcatgtgt acacgtaagc tccgcggtct cggcccccgt ctcgcggctg accaccgcta
     7561 cgcgcgtggc gccgggttgg aacgcccgca ccacgagccg gcccgaagcg gaatcctcat
     7621 gcatgccgag gaacgaaaac gggttaccct cgtctccggc caaaagggcc gctatccgat
     7681 ccataggcgt gtcggggccg acaaccatac gctgttgggt cagcggtctc attggctttc
     7741 ctgtctccgg taagaaggac ggcaccgttg tttccttaga gaaaaaattt caagagagaa
     7801 cccaccctca aaacgataca tagatttaat cgagcccttg tcagtaacaa tagttttacc
     7861 aaaataggat gttatgcttc gtaaaggccg tggaggaaac cagttcccag cggcggaacg
     7921 atacctatca caaacaccgc gtagggtcgc gcgaggtgtt ctcaatgcgg gtgctttttg
     7981 taacggcaga agcctacccc ctcgccaaga ccggggggct tgccgacgta tccagggctc
     8041 tgccgatcgc actcgcgcgt tcaggtatcg atatccggtt gctgcttccc ggctacccgc
     8101 gtgcctatac ccgcctcgaa cggccgcgca tagtcgccaa gctcgaaccc acgcttggaa
     8161 tcaccgacgc cacgctcatc gcgggcacct ttccgggcac ggatctgccc gtctggctga
     8221 tcgattctcg cacccttttc cggcgcaacg gcggcctcta ccaggacgaa aacggccggg
     8281 attggcccga caacgccttg cggttcgctt tcctcgccca tgtcggggcg caattggcga
     8341 tgggacgaac caaggtcgcc tggaagcccg acatcgtgca tgccaacgac tggcacaccg
     8401 gccttctgcc gctgatcctg gcccatgaaa agggccccag gccgagctgc gtgttcacca
     8461 gccacaatat ggccttccag ggaaatttcc cggccgaggt tctgccgtcg ctgaacatcc
     8521 ctgagagctg cttcaccacc aacggcatgg aattctacgg gcaggtttcg ttcctgaagg
     8581 ccggcctcag ctacgccgat cgcgtcacca ccgtcagccc aacctatgcc atggagatcg
     8641 tcacccccga attcggctgc ggcatggacg gcatcctgcg cgcgcgcggc agcaatttct
     8701 cgggaatcct caacggtatc gacgacgcgc tgtgggaccc ggccacggat ccccatctgc
     8761 ctcagaccta tcgggcgatg gacatttccg gcaaacgagt ctgcaaggcc gcgttacaga
     8821 acgaactcgg cttgccggcg agcccggaca cgccgctcat cgggttcgtc agccgcctga
     8881 cgcagcagaa aatggccgat gtgatcttgg aggccctgcc ttggatcgcc gagcaaaagg
     8941 cgcaattggc gctcgtcggc gagggcgatc cgtcattgga agaggcgttc accgcggctc
     9001 aggtccgcta cggcgagaac gttgccgtca atatcggata cgacgaaccc ttggcccacc
     9061 ggcttcaggc cggttgcgac atccttctcg cgccggcacg gttcgagcct tgcggcctga
     9121 cccagcttta cgccctgcgc tacggttccc tgcccgtcgt ccgccgcacg gggggattgg
     9181 ccgacacggt cgtggacgca accgccgaga cgatcgccaa ccggactgca accggattcg
     9241 tcttcgaggg cgccgatatg cccagtctga tatcggccac cggcagggca ttggcattct
     9301 atcgcgagcc gctgacctgg cgccggttgc agcttcacgc catggcgcag gatttcagct
     9361 ggaacgccag tgcggccaaa tacgccttgc tgtacagcga agcgagcggc gtgcct
//
LOCUS       DASWPU010000551         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_72309,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000551 DASWPU010000000
VERSION     DASWPU010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72309"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VGC27_11590"
     CDS             complement(<1..341)
                     /locus_tag="VGC27_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0283253.1"
                     /translation="MLTRLSIRDIVLIDRLDIDFASGLAVLTGETGAGKSILLDAFAL
                     ALGARGDASLVRQGTEQGQVTASFDVPAGHPARDLLAANDIAAEDELILRRVQLADGR
                     TRAFVNDQPVGV"
     gene            complement(626..>1567)
                     /locus_tag="VGC27_11595"
     CDS             complement(626..>1567)
                     /locus_tag="VGC27_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057843149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0283254.1"
                     /translation="TLGAFRVENADLLDRLLSEHLAALAEARLIDLDTLAQDGVRIRA
                     SAGAASFRREATLDRHLALAEAVVEELKREVETSSDAGNQRIKAAKERAAHERSERVK
                     AAQSALAEIKRQRQVREEKRGNGKKPKEPRASTTDADARVMKMADGGFRPGYNVQVAS
                     VAGEQIVVAVEVCNNGSDRGLMRPMLERVRALMGRGPKRHLADGGFGSADDIEWAHGN
                     GIEVYCPPTQSKHGTDPYRLRRGDGPGVSAWRARMASEAGKAQYKPRSICECIHARWR
                     NWHLRQLPVRRAEKVRAIVLWYALTNNILQGHRLAHR"
BASE COUNT          252 a          512 c          527 g          276 t
ORIGIN      
        1 acgccgaccg gctggtcatt gacgaaggcg cgcgtgcgcc cgtcggcgag ctgcacgcga
       61 cgcaggatca gctcgtcctc ggcggcgatg tcgttggcgg cgagcaggtc gcgcgccgga
      121 tggccggccg gcacgtcgaa cgaggccgtg acctgtccct gctcggtgcc ctgacgcacc
      181 aggctcgcat cgccgcgcgc accgagcgcc agcgcaaagg cgtcgagcag gatcgacttc
      241 ccggcgccgg tctcgccggt cagcaccgca aggccggagg cgaaatcgat gtcgaggcga
      301 tcgatcagga cgatgtcgcg gatcgagagc cgggtcagca tggccactcc gcaagctcag
      361 ggaaggcgca agactagagg attcgaatcg gcgcgtctcg cgcggaaaat gtatgtggga
      421 aacgtgtcat tgaaagcgtg gcgaagcgcc gaaacttgct ccacctcatc ctgagaggct
      481 gcgaagcttt tcggattttg gcgtgccgga ggcgaggaat cgagtctccg tcgctcggat
      541 gcaagcgacg catcagcgag cattggctgg tgcggtatgc aggcggcctt ttgacgtggc
      601 ggcgagtttg agaggggtcg tcctcctatc ggtgggcgag acgatgacct tgcaggatgt
      661 tgttggtgag ggcgtaccag agaacgatgg ctcggacctt ttcggcgcgg cgcaccggca
      721 gttgccgcag gtgccagttg cgccagcgtg catggatgca ttcgcagatg gagcgcggtt
      781 tgtactgcgc cttaccggcc tcgcttgcca tgcgggctcg ccacgccgac acgccggggc
      841 cgtcgccacg gcgcagccga tagggatcgg tgccgtgctt ggactgggtg ggcggacaat
      901 agacctcgat gccgttgcca tgtgcccatt cgatatcatc agcgctgcca aacccgccgt
      961 ccgcgagatg gcgcttggga ccacggccca tgagcgcgcg cactcgttcc agcatcgggc
     1021 gcatgagacc gcggtcggag ccgttgttgc agacctcgac agcgaccacg atctgctcgc
     1081 cggctacgct tgccacctgc acgttgtatc ccgggcggaa gccgccatca gccatcttca
     1141 tcacccgtgc gtcggcatcg gtcgtggagg cgcgcggctc cttgggcttc ttgccgttgc
     1201 cgcgcttctc ctcgcgcacc tgacgctgcc gcttgatctc agcgagcgcg gactgcgccg
     1261 ctttcacgcg ctcgctgcgt tcgtgcgcgg cgcgctcctt ggcggccttg atgcgctgat
     1321 tgccggcatc cgaacttgtc tcgacctcac gcttgagctc ctccaccaca gcttcggcca
     1381 gggccaagtg gcgatcgagc gtcgcctcgc gccggaacga ggcagcgccc gcgctcgccc
     1441 gtatccgcac cccatcctgc gccaaggtat cgagatcgat cagacgggcc tcggccagcg
     1501 ccgccaaatg ctcgctcagc agccgatcga gcagatcggc attttcgacc cgaaaggctc
     1561 ccagggt
//
LOCUS       DASWPU010000552         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_77970,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000552 DASWPU010000000
VERSION     DASWPU010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77970"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1175)
                     /gene="lipA"
                     /locus_tag="VGC27_11600"
     CDS             complement(210..1175)
                     /gene="lipA"
                     /locus_tag="VGC27_11600"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY0283255.1"
                     /translation="MTTVFDQIEKDRRAALRHPEKARNPDSSVLRKPDWIRVKAPLGR
                     EYTQTEAVVREHGLHTVCEEAGCPNIGECWTRRHATMMIMGDTCTRACAFCNVKTGLP
                     GPLDAGEPARVADAVARLGLKHVVVTSVDRDDLPDGGAKHFAETIQAIRKASPGTTVE
                     VLTPDFLRKPGAIETVMEARPDVFNHNLETVPRLYLNVRPGARYYVSLRLLDRAKELA
                     PEGFTKSGLMVGLGESREEIMQVMDDLRAAGVDFLTIGQYLQPTRKHAPLDRFWSPEE
                     FKALEATARAKGFQMVSASPLTRSSYHADSDFAALKVARSVSFPP"
     gene            1275..>1927
                     /locus_tag="VGC27_11605"
     CDS             1275..>1927
                     /locus_tag="VGC27_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0283256.1"
                     /translation="MAAASVATASGKELFPLDLEQTKKTVFQALIEARSRFGGTRPAI
                     IDGDKRVLTYDEIVRAALALGHALKKGTARGESVGIMLPTSAAAVIAFFAVSAFGRIP
                     AMLNFTAGAAGVMSALRTAKVKCIVTAHRFIELAKLDALAAEISMASALVYLEDVREK
                     LSIFDKAVAGIGQFLPNLVTSRPAHGSTAVILFTSGTEGEPKGVALSHSNLMANVVQV
                     "
BASE COUNT          347 a          609 c          607 g          364 t
ORIGIN      
        1 cgcagcacgt cgcgatcctt gacctgttcc ctcgccagaa cccgcagggc gacgcaccaa
       61 ggcagaaatt cgggatattt ctccacctcg gccaccacgg cgtacatcag gtcggccgtg
      121 taaggaagaa tgcgggattc agagtgctgc gtcatccacc ccccttccgt ccacctacgc
      181 gctacgcgct tcggcggaca ccttccccct caagggggga aggaaacact ccgcgcaact
      241 ttcagcgccg cgaagtcgga atcggcgtga taggacgagc gcgtcagcgg actggccgac
      301 accatctgga agccctttgc gcgcgccgtg gcttccagcg ccttgaattc ctccggcgac
      361 cagaagcggt cgagcggcgc atgcttgcgc gtgggctgga gatattggcc gatggtgagg
      421 aaatcgacgc cggccgcgcg cagatcgtcc atcacctgca tgatctcttc gcggctctcg
      481 ccaagaccca ccatcaggcc ggatttggtg aaaccttcag gggcaagttc cttggcgcgg
      541 tcaagcagac gcaggctgac gtaatagcgc gcgccggggc ggacgttgag gtagagccgc
      601 ggcacggtct caagattgtg gttgaagacg tcgggccggg cctccatcac ggtctcgatg
      661 gcgccaggtt tacgcaggaa gtcgggtgtc agcacttcca ccgtcgtgcc ggggctggct
      721 ttgcggatgg cctgaatagt ttcggcaaaa tgttttgctc cgccgtcggg cagatcgtcg
      781 cggtccaccg aggtgacgac gacatgctta agcccaagtc tggcgaccgc atccgcgact
      841 cgcgctggtt cgccggcatc cagcggaccc ggcaggccgg tcttgacgtt gcagaaggcg
      901 caggcccgcg tacaggtatc ccccatgatc atcatggtgg cgtggcggcg ggtccagcat
      961 tcaccaatat tggggcaccc ggcctcctcg cagacggtgt gaagcccgtg ctcgcgcacg
     1021 acggcctcgg tctgtgtata ttcgcgcccc aagggcgctt tgacgcggat ccaatcaggt
     1081 ttgcgcagga ctgagctgtc cggattgcgc gccttttcag ggtgacgtag cgccgccctg
     1141 cggtctttct cgatttggtc gaaaactgtg gtcatggtcc tgtacatata gcttactcta
     1201 ccgaaaatca cccggttccg ttaggttgcc gtcataacta tgagggcaaa gcccccatct
     1261 tcaggaggaa tcagttggcc gccgctagcg ttgccaccgc atcgggaaag gaactcttcc
     1321 cgctcgacct ggagcaaacc aagaaaacgg tattccaggc gctgatcgag gcccggtccc
     1381 gtttcggggg cacccgcccc gccatcattg acggcgacaa gcgtgtcctg acctacgacg
     1441 agatcgtgcg cgcggcgctg gccctcggcc atgccttgaa gaaaggaacg gcgcgaggcg
     1501 aatccgtggg catcatgctg ccgaccagcg cggccgcggt gatcgccttt tttgcggttt
     1561 ccgccttcgg gcgcatcccg gcgatgctga acttcaccgc cggcgcggcc ggcgtgatga
     1621 gcgcgctgcg gaccgccaag gtcaaatgca tcgtcacggc gcaccggttc atcgagctgg
     1681 ccaaattgga cgcgctggcc gcggaaattt ccatggcgtc ggcgctggtc tatctggagg
     1741 acgtccgcga gaaattgtcg atcttcgaca aggccgttgc gggcatcgga cagttcctgc
     1801 ctaacctcgt cacctcgcgg ccggcgcatg gatcgacggc ggtcatcctg ttcacctccg
     1861 gcaccgaagg cgaaccgaag ggcgtggcgc tgtcgcattc gaacctgatg gccaacgtcg
     1921 tgcaggt
//
LOCUS       DASWPU010000553         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_90409,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000553 DASWPU010000000
VERSION     DASWPU010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90409"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VGC27_11610"
     CDS             complement(<1..526)
                     /locus_tag="VGC27_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283257.1"
                     /translation="MGLPPKQGLYDPRNEHDSCGVGFVANIKGVKSHGIITQGLQVLS
                     NLDHRGAVGADPLVGDGAGILIQIPDALFRAWAAENGVTLPKPGHYAIAMCFLPREDT
                     ARAIAVAQFEHFLRVEGQVLLGWRDVPTDPTGLGETVISEMPLIRQALIGWGPNIRDE
                     GAFERKLLAVRKQTQ"
     gene            931..1977
                     /locus_tag="VGC27_11615"
     CDS             931..1977
                     /locus_tag="VGC27_11615"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEY0283258.1"
                     /translation="MGFKDTTETRTAAGTAKPGRQRSWAEPWKVKVVEPIRVMTREER
                     ERAIAAAGYNTFLLRSEDVHIDLLTDSGTSAMSDWQWAGMMQGDEAYAGSRNFYHLEE
                     AVREYYGYKHMVPTHQGRGAENLLSKMLIKPGDTVPGNMYFTTTRLHQELAGGSFADV
                     IVPQAHNPADEHPFKGNVDLNALESLIQKAGAKRIPYVCVAATVNMAGGQPISMANMK
                     AVRALTARHGIRIFLDATRAVENAYFIQQREPGHRERGVAAILKEFCSYSDGCTMSGK
                     KDSLVKIGGWLALNDDTLYEDASNLVVVYEGLHTYGGMAGRDMEAMARGIVSRWTTTI
                     SAPASARWNISATS"
BASE COUNT          404 a          632 c          639 g          350 t
ORIGIN      
        1 tctgcgtctg cttgcggacg gcgagcagct tgcgctcgaa ggcgccctcg tcgcggatgt
       61 tcggccccca gccgatgagc gcctggcgga tcagcggcat ttcggaaatg acggtttcgc
      121 ccagtccggt gggatcggtc ggcacgtcgc gccagccgag caggacctgc ccctctactc
      181 tcaggaagtg ctcgaattgc gcgacggcaa tggcgcgcgc agtgtcttcg cgcggcagaa
      241 agcacatggc aatcgcataa tggcccggct tgggcagcgt gacgccgttc tcggccgccc
      301 aggcgcgaaa cagcgcatcg ggaatctgga tcaggatgcc ggcgccgtcg ccgaccagcg
      361 ggtccgcgcc gaccgcgccg cgatggtcca aattggagag aacctggagc ccttgggtga
      421 taatgccgtg ggatttcacg cccttgatgt tggcgacaaa gccgacaccg cacgagtcgt
      481 gctcgtttcg cggatcgtac aggccttgtt tcggcggcag tcccatttgc ttgtatccag
      541 cagagtcaat tcagtccccc ggcggaagcc ggaatttcca tccatacata agctgtccgg
      601 atcggttctg acaacaaaag ttattgcgtg gtagtgacgc attttgtaat ctaattgcgc
      661 aaaaacgata catctttgcg gcgcacggcg ctcccgccac cccttgccga tttgctgccg
      721 aaagcgccat ttgctgtcgc ttacggcctg tggaccgggt gactggtcac agatattaac
      781 tggcaaaaca cggctccaaa agctccccca agcgtgtgga ggaaataccg gatcagcgcg
      841 gttcctaacc ggagctgcct agattatgga catcggtgtg gttgtgcagc agttccgtgc
      901 ctgccgcagc ggacaacaag gacagttaac atgggtttca aagacacgac agaaacgcgg
      961 accgcggccg gcaccgccaa gcctggccgc cagcgctcct gggccgagcc gtggaaagtc
     1021 aaagtggtcg aaccgatccg cgtgatgacg cgcgaagagc gcgagcgggc gatcgccgcc
     1081 gccggctaca acaccttcct gctccgctcc gaggacgtgc atatcgacct tctgaccgac
     1141 agcggcacca gcgcgatgag cgactggcaa tgggcgggca tgatgcaggg cgacgaagcc
     1201 tatgccggca gccgcaattt ctatcatctc gaagaggccg tgcgggagta ttacggctac
     1261 aagcacatgg tgccgacgca ccaagggcgc ggcgccgaaa acctgctgtc gaaaatgctg
     1321 atcaagccgg gcgataccgt gcccggcaac atgtatttca ccaccacgcg gctgcatcag
     1381 gagctggccg ggggaagctt cgccgacgtc atcgtgccgc aagcgcataa tccggcggac
     1441 gagcatccct tcaagggcaa tgtcgatctg aacgccctgg agagtctcat tcagaaggct
     1501 ggagccaagc gcatcccgta tgtctgcgtc gccgccaccg tgaacatggc cggcggccag
     1561 cccatctcga tggcgaacat gaaggcggtg cgcgcgctga ccgcccgcca cggcatccgg
     1621 atctttctcg acgcgacgcg cgccgtcgag aacgcctatt tcatccagca gcgcgaaccc
     1681 ggccaccgcg agcgcggcgt cgccgccatc ctcaaggagt tctgttccta cagcgacggc
     1741 tgcaccatga gcggcaagaa ggactcgctg gtcaagatcg gcggctggct ggcgctcaac
     1801 gatgacacgc tctacgagga tgccagcaat ctcgtcgtcg tctatgaagg cctgcacacc
     1861 tatggcggca tggcgggacg cgacatggaa gccatggcgc gcgggatcgt gagtcggtgg
     1921 acgacgacca tatccgcgcc cgcgtcggcc aggtggaata tctcggcaac aagctgatcg
     1981 cggcgggggt gcccatcgtg cggccggtcg gcgggcacgc ggtct
//
LOCUS       DASWPU010000554         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_10357,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000554 DASWPU010000000
VERSION     DASWPU010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10357"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>2629
                     /locus_tag="VGC27_11620"
     CDS             23..>2629
                     /locus_tag="VGC27_11620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0283259.1"
                     /translation="MIVGILAILKAGCAYLPIDRDCPADRVLTMLEDAGAGKVLLAGG
                     ASWQDEKRLVFNLDDSALYRGNAANIAGRTESRSLAYVIYTSGTTGVPKGVMIEHHSV
                     NNLCVWFNHEFSVTAADRGTKCARFSFDASVWEIFPYLQAGAAIHVVDDEIRLDLARF
                     NRFLERNAITICFLPTQLCELFTEFDNNSLRLLLTGGDRLRRVEARTYRIVNNYGPTE
                     NTVVATSCDAAAGTECLPIGKPVFNTRVYILRDGTELVPVGVPGELCISGAGLARGYL
                     HDPEKTAEKFVPNPFEPGAVMYRTGDLARWLPDGRIAYIGRIDNQVKIRGCRVEPREI
                     EVAILSHQTVKDAVVVARQDSRNTNVLHAYVVWHGVPTPAELRAHLAKRLPDYMVPAF
                     IVTVDEIPLNPRGKIDPTRLPKAEGEVSRDLVMARTNTERRLADIWNRVLDRERVGIH
                     DNFFEIGGDSLRATIMLARANKEFEADVALGAVFDNPTVAALARCYDGVTRHRSTAIG
                     TAEPRLYYPTTPTQSLLYAVCNATKGVEYNLPFVFELRGDLNVERLEAAFRRLIERHE
                     AFRTSFHMVGGSLMLTVSPSADFAIDILPPCSESDIEEVARDFIRPFDLGRAPLLRVA
                     LLPLGPAHHVLLIDVHHLVSDGTSMGLMYQEVAALYAGDEPPLPATTFKDFAVWLKDH
                     LQTKRVNEQEKYWLEVFKEPLPVPRLDTDYPRPKTPSFVGNRLHFTVGPELHGGLRAI
                     CAEHGATLYMVLLAAYDILLSRYTGREDIIVGVPTAGRYVADVQDVIGMFVSTHATRN
                     RVRAELRFDEFLEQVKSGVLGALENQEYQLWSLMIAHTLKNSGSPLFGSVFVVQDQAF
                     VAMRMPGLTVEER"
BASE COUNT          493 a          833 c          806 g          497 t
ORIGIN      
        1 tatcgcgggt ccttcggtcg aaatgatcgt gggcatcctc gccattctga aggcgggatg
       61 cgcctatctg ccgatcgacc gcgactgtcc cgccgaccgg gtgctgacga tgctggagga
      121 cgccggggcc ggcaaagttc tgctcgcagg gggagcaagc tggcaggacg agaagaggct
      181 cgttttcaat ctggacgatt ccgcgctcta tcgcggcaat gccgcgaaca ttgccgggcg
      241 caccgagagc agaagcctcg cctacgtgat ctacacgtca ggcaccacgg gggttcccaa
      301 gggtgtgatg atcgagcacc attcggtgaa caacctctgt gtctggttca atcacgaatt
      361 ctccgtgaca gcggcggacc gtggcacgaa atgcgcccgc ttcagcttcg acgcctcggt
      421 ctgggagata ttcccctacc tgcaggccgg cgccgcgatc catgtcgtcg acgacgagat
      481 ccggctggat ctggcgcgct tcaaccgctt cctcgagagg aacgccatca cgatctgctt
      541 cctgccgacc caactttgcg agctgttcac cgagtttgac aacaattcac tgcggcttct
      601 cctcaccggc ggcgatcgcc ttcgccgcgt cgaggcgcga acctaccgca tcgtgaacaa
      661 ctacgggccg accgagaaca cggtggtggc gacgagctgc gacgccgccg ccggcacgga
      721 gtgccttccg atcggcaaac ccgtgttcaa cacgcgcgtc tacatcctgc gggacggcac
      781 cgaacttgtt cccgtcgggg ttcccggcga gctctgcatc tctggcgccg ggctggcgcg
      841 gggctatctc cacgacccgg aaaagacggc cgaaaagttc gttcccaacc cgttcgagcc
      901 cggggcagtg atgtatcgaa cgggtgatct ggcgcggtgg ctgccggatg gccgcatcgc
      961 gtatattggg cgcatcgaca atcaagtgaa gatcagggga tgccgcgtcg agccccgcga
     1021 gatcgaggtg gcgatccttt cgcaccaaac cgtcaaggac gcggtcgtcg tcgcccgcca
     1081 ggacagtcgg aacaccaacg tgcttcatgc ctatgtggta tggcacggcg tgcccactcc
     1141 ggccgaactt cgcgcccacc tcgccaagcg ccttcccgac tatatggtgc ccgccttcat
     1201 cgttacggtc gacgagattc cgctgaaccc gcgcggaaaa atcgatccga ctagactgcc
     1261 gaaagccgaa ggcgaagtct cgcgcgatct ggtgatggcg cggacgaaca ccgaacggag
     1321 gctcgcggac atctggaacc gcgtgctgga ccgtgaacgt gtcggcatcc acgacaactt
     1381 cttcgagatc ggaggcgatt cccttcgcgc cacaatcatg ctggccagag cgaataagga
     1441 attcgaggca gacgtcgccc tgggcgcagt tttcgacaat ccgacggtgg ccgcgctggc
     1501 acgctgttat gacggcgtca cgcggcacag atcgacagcg atcggtacag cggagccccg
     1561 gctatattat cccaccacgc ccacccagtc ccttctctat gccgtatgca acgcaacgaa
     1621 gggcgtcgaa tacaaccttc cctttgtctt tgagttgcgc ggcgatttga acgtcgagcg
     1681 gcttgaagcg gcattccgcc gcttgatcga gcggcacgaa gccttccgaa cctcgttcca
     1741 tatggttggc ggttcgctca tgctgacagt aagcccatcg gcagatttcg ccatcgatat
     1801 cctgccaccc tgcagcgaga gtgatattga ggaggtcgcg cgcgacttca ttcgcccctt
     1861 cgatctcggg agggcgccgc tgctgcgcgt cgccctgctg ccgctgggac ctgcgcacca
     1921 tgtgctgctg atcgacgtcc accatctggt gagcgacggc acctctatgg gactcatgta
     1981 ccaggaagtg gccgcgcttt acgcgggtga cgagccgccg ctgcccgcga cgacgtttaa
     2041 ggatttcgcg gtatggctga aggaccatct gcagacgaag agggtgaacg agcaggagaa
     2101 gtactggctc gaggtgttca aggagccgct gccggtgccg cggctggaca ccgactatcc
     2161 ccgtccgaag acccccagct tcgtcggcaa ccggctgcat ttcaccgtgg ggccggagct
     2221 ccacggcggt ctcagggcga tctgtgccga gcacggtgcc accctctaca tggtcctgct
     2281 cgccgcgtac gatattctcc tctcccgcta cactggccgc gaggacatca tcgtcggggt
     2341 tccgacggcc ggacgctatg ttgccgacgt ccaggacgtg atcgggatgt tcgtcagtac
     2401 ccacgccaca cgcaatcgcg tgcgcgccga gcttcgcttt gacgaattcc tggaacaggt
     2461 caagagcggc gttctcggtg ccctggaaaa tcaggagtac cagctctgga gcttgatgat
     2521 cgctcatacg ctgaagaata gcggcagccc gctgttcggc tcggtgttcg tcgttcagga
     2581 tcaggcgttc gtggccatgc ggatgccagg attgaccgtc gaagaacgg
//
LOCUS       DASWPU010000555         5487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_4520, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000555 DASWPU010000000
VERSION     DASWPU010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4520"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1243)
                     /locus_tag="VGC27_11625"
     CDS             complement(146..1243)
                     /locus_tag="VGC27_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0283260.1"
                     /translation="MNSAKKPAGQEAPRPQAATSNSDRSYKRSTINDIARIAKVSKKT
                     VSRVINDSPFVKEETRLKINAIIGELGFAPDPQARGLAFRRSFLIGMIYDNPTAQYIV
                     NMQQGILDAMRDTSFELVLRPCNRAAPTFLQDMRRLIERQKLFGVILTPSVSEDDRLV
                     RMLREIDCPYVRVAAVSLDTPATMVVTHDFSGAADAARHLADLGHKIIAHISGPATFR
                     SAHERRRGFVAGLAERGIKFVRNYDREGAYTFETGHALAAELLSLKPRPTAIFAGNDE
                     MAAGVYMAARDLGLEIPKDLSVVGFDDSPVASRMWPLLTSVRLPIRDMGRMAAEKLFH
                     RRTEKRAQKDDVTEVIPTLVVRDSTGRPSGA"
     gene            complement(1453..2832)
                     /locus_tag="VGC27_11630"
     CDS             complement(1453..2832)
                     /locus_tag="VGC27_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283261.1"
                     /translation="MRSLAITLTAALCAAVAADAAATRHATPEPVRHPQTVAAAPLLA
                     FPGAMGWAATTPGGRGGRIIKVTTLANDGPGSLREAVNAKGPRIIVFEVGGVIDLDRK
                     TLTIRENNVTIAGQTAPSPGITLIRGGIDVGAHDVVLQHLRVRVGEAGQPKKSGWEED
                     AFSTVGGAMNVIADHCTFTWATDENMSISGRRFVGDTPDEWRAATSHRITLSNNIAAE
                     GLSWSTHAKIEHSKGSLIHDNATEILIVGNLYAHNYERNVLMKGGTRIEMVNNLIFNP
                     GQRIIHYNLMAEEWGDHPYQLGQLTAVGNVAQLGQSSPAKVAFLDLGGYGDLLYFGKD
                     NIAVDQIGRPIPTLGRYTTSPAKIIVAKAPPLSAPYVKPMAASAVQEYVLHNAGARPW
                     DRDYDDVRLIADVAEGRGTIIDSENDVQGYPVGKPTQRPFNPADWNLIDMTPKSPSAL
                     DSSAKAHGT"
     gene            2983..3825
                     /locus_tag="VGC27_11635"
     CDS             2983..3825
                     /locus_tag="VGC27_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283262.1"
                     /translation="MTEVGVSMEAINPVRFAPAGRIAQRFVAARLNAMALAEFPGPIP
                     PDMASGYDCQEAALALWPEEIVGWKIGRIPPAFESKLGQTRLAGVIFPSGVQRATAEV
                     EFGVFAGGFAAVEAEYIYEIAKDAPAGQLRWSPSEAAAIAGRMFVGIETAGSPLATIN
                     VLGPTVVVSDFGNNAGLILGPEVPNWRDREPVSLTCESFIDDKPVGQGTAANIPGGPL
                     GALQFILEHCAKRGRPLKEGMLISTGAATGIHDIVAGQKARAEFKGIGTVCGLAKPRA
                     AQKP"
     gene            3887..5218
                     /locus_tag="VGC27_11640"
     CDS             3887..5218
                     /locus_tag="VGC27_11640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0283263.1"
                     /translation="MTQLGSPASAAGTPQPAPRVGHYRWVICAMLFAATGLNYVDRQT
                     IGFLKPALSAEYGWDQTDYAHIVQWFMIAYAFGYVVFGRVIDKIGAKLGYSIAVVIWT
                     VAHLCCALLGVLPPALMLTSFMVTQAALGLGQSGNFPAALKAVAEWFPQRERALANGI
                     FNAGTNVGAIVTPLIVPVVMLNFGWQWAFVATGSLSVIWLILWVWVYQKPSENKHLTQ
                     AERDYIESDKPEPVKQLPWLKVMRVKETWAYALGKLLTDPVWFFYLFWLPPYFFETYK
                     DQVGNIAGVAAPIIVIYLISDVGSVAGGWLSSAMIKAGASVNMARKLTMLLCAACVLP
                     VGFATMFVHDLWTVTFVIGIAAAAHQAFSANLYTIPSDTFPKAAVGSVSGIGGTAGAI
                     GGIIMTMGVAQALTGLGGYSSVFFVAGLIYFLAILVIHVLTPRLAPAKLEA"
BASE COUNT          950 a         1721 c         1747 g         1069 t
ORIGIN      
        1 gtccgccgac gccatactgt cggggtgcgt cgcttggtag atcttcttgt acatcgcctt
       61 acctctatgc tgttcgcgcg tcccatgcgc cccttgattt ggtaaccggt gtcatacgcc
      121 caggaccaac acccgtgcaa cgcctttatg cgccagacgg gcgccccgtg gaatcgcgca
      181 ccacaagtgt cgggatgacc tcggtaacgt catccttctg ggcgcgcttt tccgtgcggc
      241 ggtggaacag cttctcggcg gccatccgcc ccatgtcgcg gatcggcagg cgcaccgagg
      301 tcaacagcgg ccacatgcgc gatgcgacgg gactgtcgtc aaagccgacg accgaaagat
      361 cctttggaat ctccagaccc agatcgcgag cggccatgta aacaccggcc gccatttcgt
      421 cgttgccggc gaagatggcc gtcggcctcg gcttcagcga aagcagctcc gccgccaagg
      481 catggccggt ctcgaaggtg taggccccct ctcgatcata gttgcggacg aacttaattc
      541 cgcgttcggc aagccccgct acaaagccgc gccggcgttc gtgggccgag cggaaggtcg
      601 cgggcccgga aatatgcgcg atgatcttgt ggccgagatc ggccaagtgg cgcgcggcgt
      661 cggcggcgcc ggagaagtcg tgagtgacca ccattgtcgc cggcgtatcg agcgacaccg
      721 cggcgacgcg cacataagga caatcgatct cgcgcagcat gcgtacgagc cggtcgtcct
      781 ccgacaccga cggcgtcagg atgacgccga acagcttctg ccgctcgatc aggcggcgca
      841 tgtcttgaag gaacgtcggc gcggcgcggt tgcagggccg gagcaccaat tcgaaactgg
      901 tgtcgcgcat cgcatcgaga atgccctgct gcatgttcac gatgtattgg gcggtggggt
      961 tgtcgtagat catgccgatc aggaacgaac gcctgaaggc gaggccgcgg gcctgcgggt
     1021 cgggcgcgaa gccgagttcg ccgatgatcg cgttgatctt gaggcgcgtt tcttccttca
     1081 cgaagggcga atcgttgatc acccgcgaca cggtcttctt cgacaccttg gcgatgcgcg
     1141 cgatgtcgtt gatcgtcgat cttttgtagc tgcggtcgga attggaggtc gccgcttgcg
     1201 ggcgcggtgc ctcttgcccg gctggctttt tcgctgaatt cacactctat acgggtatgg
     1261 gtcccgcccc ttattagctc tcggccggct tcatgggaat gagagaaaat cccccggctg
     1321 ctgatttgat accggtaaca gtatcagcca cagacagggt tttccatcaa gggaaaggca
     1381 ttatgcctgc cgcttgacgc gccgcagtcc caggccagcc cggacagcct aagcgagtat
     1441 tccgtcgcag aatcaggtcc cgtgcgcttt ggcgctggag tccagagcgc tcggcgactt
     1501 cggcgtcatg tcgatcaggt tccaatcggc gggattgaac gggcgctgcg tcggcttccc
     1561 gaccggatag ccctggacgt cgttctcgct gtcgatgatg gtgccgcggc cctcggcgac
     1621 gtcggcgatc agccgcacat cgtcataatc gcgatcccaa ggacgtgcgc cggcattgtg
     1681 cagaacatat tcctgtaccg cagaggccgc catcggcttt acgtaaggcg cactcagcgg
     1741 gggcgccttc gccacgatga tcttggcggg cgaggtggtg tagcgcccca aggtcgggat
     1801 cggtcggccg atctggtcta cggcgatgtt gtctttgccg aaatagagca ggtcgccgta
     1861 gccgccgaga tccagaaagg cgaccttggc cggcgacgat tggccaagct gcgcgacgtt
     1921 tccgaccgcc gtcaactggc cgagctgata aggatgatcg ccccattcct cggccatcag
     1981 attgtagtga atgatgcgct ggccgggatt gaagatcagg ttgttgacca tctctatgcg
     2041 cgtgccgccc ttcatcagga cgttgcgctc atagttatgg gcataaaggt tgcccacgat
     2101 cagaatctcg gttgcgttgt cgtggatgag cgaacccttg gaatgctcga tcttggcgtg
     2161 ggtggaccag gacagccctt cggccgcaat attgttactg agggtaatgc ggtgcgaggt
     2221 tgccgcgcgc cattcgtcgg gcgtgtcgcc gacaaaacgc cggccggaga tcgacatgtt
     2281 ctcatctgtt gcccaggtga aggtgcagtg atcggcgatc acgttcatcg ccccaccgac
     2341 ggtcgagaag gcgtcctctt cccaaccgct tttcttgggc tgtccggctt cgccgactct
     2401 caccctcagg tgctggagca cgacatcgtg ggcgccgaca tcgatgccgc cgcggatgag
     2461 cgtgattccc ggagacggcg cggtttggcc cgcaatggtg acgttatttt cgcggatggt
     2521 gagcgtcttc cggtccaggt cgatgacccc gccgacctcg aacacgatga tgcgcggccc
     2581 tttggcgttg acggcctcac gcaacgagcc cggcccgtcg ttggccaggg tggtgacctt
     2641 gatgattcgt ccgccgcgtc cgccgggggt ggtcgccgcc cagcccatgg cgccggggaa
     2701 ggccagtaag ggcgcagccg caacggtctg gggatgccgg accggctcgg gggtggcgtg
     2761 tcgcgtcgcc gcagcatcgg ccgccacggc ggcacagaga gcggctgtca aggtgatggc
     2821 taagctgcgc aaagtctttt cctcctgcgt tttcccggcg tttgacggtt gggcagccgg
     2881 gcggcacgct cggttcccca ccatagagcc tttttaatcc tgccgattga aaatgacacc
     2941 ggtttcctct agacacaagc ttcctaagtt gccgaggaga tcatgacgga ggttggcgtg
     3001 tccatggaag cgatcaatcc ggtgcgcttc gcaccggcag gtagaatcgc gcagcgcttt
     3061 gtcgcggcgc ggttgaacgc gatggccctc gccgagtttc ccgggccaat cccccccgac
     3121 atggcaagcg gctacgattg ccaggaggcc gcgctcgcac tgtggccgga agaaatcgtt
     3181 ggctggaaga tcggacgcat tccgcccgcc ttcgagtcga agctcggcca gacgcggcta
     3241 gccggcgtca ttttcccctc cggggtgcaa cgggcgacgg cggaggttga attcggtgtg
     3301 ttcgccggcg gtttcgccgc ggtcgaagcg gaatacatct acgaaatcgc caaggacgcg
     3361 cctgccgggc agttgcgctg gtcgccaagc gaggccgccg ccatcgccgg acgcatgttc
     3421 gtgggcatcg agaccgcggg cagcccgctc gccaccatca acgtactcgg accgacggtc
     3481 gtcgtttccg atttcggcaa taacgccggc ctgatcctcg gtcccgaagt tcccaactgg
     3541 cgcgaccgcg agcccgtaag tttaacctgc gagagcttca tcgacgacaa gccggtcgga
     3601 caaggtaccg ccgccaacat ccccggggga ccgctcggcg cattgcagtt catcttggag
     3661 cactgcgcca agagggggcg gccgcttaaa gaaggaatgc tgatctcaac cggcgcggca
     3721 accggcatcc acgacatcgt cgcgggccag aaggcgcgcg ccgagttcaa aggcatcgga
     3781 accgtctgcg gattggcgaa gccgcgcgcg gcgcaaaagc cgtaaacggt cccgcacagg
     3841 caatgcagga gcggccagct aaggccgccc gctcagggag tgcgagatga cccaattggg
     3901 ctcgcccgcg agcgccgcgg gaacgccgca acctgcgccg cgtgtcggcc actatcgctg
     3961 ggtgatctgc gcgatgttgt tcgccgccac cgggctgaac tatgtcgacc ggcaaaccat
     4021 aggcttcctc aagccggcgc tgtccgcgga atacggatgg gaccagaccg actacgctca
     4081 tatcgtgcag tggttcatga tcgcctatgc cttcggctat gtggtgttcg gccgggtgat
     4141 cgacaagatc ggcgccaagc tgggctactc catcgccgtc gtgatctgga cggttgcaca
     4201 tttgtgttgc gccctcctcg gcgtcctgcc gccggccttg atgctgacaa gcttcatggt
     4261 gacccaggcg gcgctcggcc tcggccaatc gggcaacttc cccgccgcgc tgaaagcggt
     4321 ggccgaatgg ttcccgcagc gtgaacgcgc cctggccaac ggcatcttca acgccggcac
     4381 caatgtcggc gccatcgtca cgccgctgat cgttcccgtc gtcatgctga acttcggctg
     4441 gcaatgggcg ttcgtcgcca ccggttcgct cagcgtgatc tggctcattc tgtgggtctg
     4501 ggtctatcag aagccttcgg agaacaaaca ccttacgcaa gccgagcgcg actatatcga
     4561 gagcgacaag cccgaaccgg tgaagcagct tccctggctc aaggtgatgc gggtgaagga
     4621 aacctgggcc tacgcgctcg gcaaactcct caccgacccg gtatggttct tctatctgtt
     4681 ctggctgccg ccttatttct tcgaaaccta caaggatcaa gtcggcaata tcgcgggcgt
     4741 cgcggcgccc atcatcgtga tctatctgat ctccgacgtc ggctcggtcg ccggcggctg
     4801 gctgtcctcg gcgatgatca aggccggcgc atcggtcaac atggcgcgca agctcaccat
     4861 gctgctgtgc gccgcctgcg tgcttccggt gggcttcgcc accatgttcg tccacgattt
     4921 gtggaccgtg acgttcgtca tcggcatcgc ggccgcggcg catcaggctt tctcggccaa
     4981 cctctacacc attccttcgg acacgttccc gaaggcggcg gtgggttcgg tctccggtat
     5041 cggcggcacc gcaggcgcca ttggcggcat catcatgacc atgggcgtcg cccaggcgct
     5101 gacgggactg ggcggctatt cgtccgtctt tttcgtcgcg gggctgatct atttcctcgc
     5161 gatcctggtc attcacgtgc tgacgccgcg cctcgcaccc gccaagctag aagcatagcc
     5221 ggcctagcca ggctcgttcc cgcctcgaga gcgggccggg cggcacggcg cagccttttg
     5281 cagatggcga ttgaattgca gcggccattt gcctaaccat ccccgcgaaa ttcagccatc
     5341 gcgcggagaa aagagtgccc gaaccagccg ttgagaatgc aggatggggc gacgtcaagg
     5401 tcgttccggt cgagggcggc gccctctggc gcgcctttca ccggttgccc tacgggctgt
     5461 acaagaacga tccgaactgg atcgcgc
//
LOCUS       DASWPU010000556         4731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_84675,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000556 DASWPU010000000
VERSION     DASWPU010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84675"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VGC27_11645"
     CDS             <1..247
                     /locus_tag="VGC27_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0283264.1"
                     /translation="KYGAIDYLPKPADADDIMAALLAPPGSKPKPPENPMSADRVRWE
                     HIQRVYELCGHNVSETARRLNMHRRTLQRILAKRSPR"
     gene            complement(248..676)
                     /locus_tag="VGC27_11650"
     CDS             complement(248..676)
                     /locus_tag="VGC27_11650"
                     /inference="COORDINATES: protein motif:HMM:NF018070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEY0283265.1"
                     /translation="MLVTAADVARGASRLLLQEGFSPILEFTLANGRRLDVAALGPDG
                     TMLGVEIKVALGDLKGDQKWPEYLEFCELFYFAIPPDFPDEYVPTGTGLIVADRYGGA
                     IVRPSPRTVLHASRRKAVTLSFAKVAAERLASILETMGSL"
     gene            complement(733..1332)
                     /gene="hisB"
                     /locus_tag="VGC27_11655"
     CDS             complement(733..1332)
                     /gene="hisB"
                     /locus_tag="VGC27_11655"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY0283266.1"
                     /translation="MRTATVERKTRETEIAVSLDLDGTGDYDVDTGIGFLDHMLESFA
                     RHSMIDLKVRAQGDLHVDFHHTTEDSAIVIGQAVKKALGDLAGITRFGAAAIAMDEAL
                     TRVAVDVSNRPYLVWKVEIPTPKLGEMDTELFKEWFQAFALNAGICLHVENLYGRNSH
                     HIVETCFKGLARALRQAVTPDERLEGGVASTKGVLSDKP"
     gene            complement(1555..2565)
                     /locus_tag="VGC27_11660"
     CDS             complement(1555..2565)
                     /locus_tag="VGC27_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283267.1"
                     /translation="MQPLVKIPYVDLGGYTPLELLFLYRRSAAALVEGVKRTFGGTSG
                     TFALPWTDRAARCWLEKSDNPYLPEIEAMAQALDLPGVYTLNLRFEWGCTSGVWHSDD
                     SPLLRRVLDWPFPALGEHTVVAHQKGPAGDFLNVTWPGLSGILQALAHGRFAAAINLA
                     PRRRRGAGLAGKWALGRFTVARNHALPPSHLLRRAFETASDYAAAKAMLCTAPVAVPA
                     IFTLSGVRRGEGCVIERTEEAFAVREMADGHVCATNHFESHLNDAGSGWLARPIGSRD
                     RLVYARTLGPAADLSWFTPPIANVNSRLALTANAVKGALMVMGTAGAEPVTETFRLPV
                     PD"
     gene            2611..3153
                     /gene="hslV"
                     /locus_tag="VGC27_11665"
     CDS             2611..3153
                     /gene="hslV"
                     /locus_tag="VGC27_11665"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY0283268.1"
                     /translation="MTDLKWRSTTILSVRKGSTVIVAGDGQVTLGNTVMKANARKVRR
                     LGKGDVIAGFAGATADAFALFERLEAKLEQHPGNLARACVELGKDWRTDRYLRRLEAM
                     MAVADATTSLVLSGTGDVVEPEDGLIGIGSGGPYAMAAARALLAFDLPAEEIARRAMK
                     IAADICIYTNENVTIESLSS"
     gene            3150..4460
                     /gene="hslU"
                     /locus_tag="VGC27_11670"
     CDS             3150..4460
                     /gene="hslU"
                     /locus_tag="VGC27_11670"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY0283269.1"
                     /translation="MISSAFTPREIVSELDRYIVGQHEAKRAVAIALRNRWRRQQLPA
                     DLREEVLPKNILMIGPTGVGKTEISRRLAKLAQAPFLKVEATKFTEVGYVGRDVEQIV
                     RDLVEVAIAQVREKKRRDVEAQAEARAEDRVLDALAKSSGGVSRESLRKALRAGEFDG
                     KEIEIEMKDAGGMPSFEIPGMPNAQISMVNLGDMFGKAFGSRSKPRRMSVAEAHAPLL
                     AEEADNLLDQDAMLREAIATVEDNGIVFLDEIDKICARSEFRGGGDVSREGVQRDLLP
                     LIEGTTVATKHGQVKTDHILFIASGAFHTAKPSDLLPELQGRLPIRVELKALTRDDFQ
                     RILTEPEASLIKQYVALLATEEVALDFTADGIGAIADIAAQVNASVENIGARRLQTVM
                     ERVLDEISFSAAERQGEKLVIDGAYVRARVEDLAKDQDLSRFIL"
     gene            4580..>4731
                     /locus_tag="VGC27_11675"
     CDS             4580..>4731
                     /locus_tag="VGC27_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0283270.1"
                     /translation="MARSSYLFTSESVSEGHPDKVCDRISDEVVDLFFREGPKHGFSP
                     WDLRVA"
BASE COUNT          878 a         1513 c         1533 g          807 t
ORIGIN      
        1 gaaatacggc gccatcgatt atctgcccaa gcccgccgac gccgacgaca tcatggcggc
       61 gctgctggcg cctcccggct ccaagcccaa gccgccggaa aatccgatgt cggccgaccg
      121 cgtgcgctgg gagcatatcc agcgcgtcta cgaactgtgc ggccacaatg tttcggagac
      181 ggcgcgccgc ctcaacatgc accggcgcac gctgcaacgc attctggcga aacgcagccc
      241 gcgctaatca gagactaccc attgtttcca aaatgctcgc cagacgctcc gccgcgacct
      301 tggcgaagct gagggtcacg gctttgcgcc gactggcatg tagcaccgtg cgcggcgacg
      361 ggcgcacgat cgccccgccg taacggtcgg cgacgatcag gccggtgccc gtgggcacat
      421 actcgtcggg aaaatcgggc gggatggcga aataaaagag ttcgcagaac tccagatact
      481 ccggccattt ctggtcgccc ttcagatcgc cgagcgcgac tttgatctcc actcccagca
      541 tggtgccgtc ggggccaagc gcggcgacat ccagccggcg gccgttggcc agggtgaatt
      601 ccaggatcgg gctgaagcct tcctgaagca aaaggcggct ggcgccgcgc gcgacatctg
      661 cggccgtgac aagcatggag cgatcataga gtgaaacggc gcggcgtcac cacgatcgcc
      721 agggaagaca attcagggtt tgtcgctcaa cacgcctttg gtcgaagcga cgccgccctc
      781 cagccgctcg tcgggcgtta ccgcctggcg cagcgccctc gccagcccct tgaagcaggt
      841 ttccacgatg tggtggctgt tccggccgta gaggttctcg acgtgcaggc agattccggc
      901 gttcagggcg aaggcctgga accattcctt gaacagttcg gtgtccatct cgcccagctt
      961 gggtgtggga atttccacct tccacaccag atagggccga ttcgagacat cgacggcgac
     1021 gcgggtcagc gcttcgtcca tggcgatggc ggcggcgccg aaacgggtga tcccggccag
     1081 atcgcccagc gccttcttca ccgcctggcc gatgacgatg gccgagtcct cggtggtgtg
     1141 gtggaaatcg acatgcaggt cgccctgggc gcggaccttg aggtcgatca tggagtggcg
     1201 ggcgaagctc tccagcatat ggtcgagaaa gccgatgccg gtatcgacgt cgtaatcgcc
     1261 ggtcccgtcc aggtccagcg aaacggcgat ctcggtctcc cgcgtcttgc gttcaacggt
     1321 ggcggtgcgc atggggcctc cgaagcaatg cccgcccgtt gatagcaagc gccgcgcccc
     1381 ggcgaaagcc ggctagtgtc ccgattccga aattcgcacc atctctatat cggcttcggg
     1441 cgaatttcgg gatcgaaagg acactagcaa tctattgaat cctagtgtcg ttttggtttc
     1501 gaagttcgct tgcagcccga tataacggat gtcgcgaact tcgaaaccac gacactagtc
     1561 gggaaccggc aggcggaagg tctcggtcac gggttcagcg cccgccgtgc ccatgaccat
     1621 gagcgcgccc ttgaccgcat ttgccgtcag cgccagccgg ctgttgacgt tggcgatggg
     1681 cggcgtgaac caggacaggt ccgccgccgg gcccagggtg cgggcgtaga caaggcggtc
     1741 gcggctgccg atcgggcgcg ccagccagcc gcttccggcg tcgttcagat gcgattcgaa
     1801 atggttggtg gcgcagacat gcccgtccgc catttcgcgg accgcgaacg cctcttcggt
     1861 gcgttcgatg acgcagcctt cgccgcggcg cacgccggac aaggtgaaga tggcgggcac
     1921 cgcgaccggc gcggtgcaca gcatcgcctt ggcggcggcg taatccgacg ccgtctcgaa
     1981 ggcccggcgc aacaggtgcg acggcggcaa ggcatggttg cgggcaacgg taaagcgccc
     2041 cagcgcccac ttgcccgcca ggccggcgcc gcgccggcgc cttggcgcca ggttgatcgc
     2101 ggccgcgaag cgcccatggg ccagcgcctg aaggatgccg ctgaggcccg gccaggtaac
     2161 attgaggaaa tcaccggccg gtcccttctg atgggcgacg acggtgtgct cgccgagggc
     2221 gggaaacggc cagtccagca cgcgccgcaa cagcgggctg tcgtcgctgt gccacacgcc
     2281 gctggtgcag ccccattcaa agcgaagatt gagcgtatag acgccgggca ggtcgagcgc
     2341 ctgcgccatg gcctcgattt cgggcagata ggggttgtcc gatttttcga gccagcagcg
     2401 cgcggcgcgg tcggtccaag gcaaggcaaa ggtgccggag gtcccgccga aggtccgttt
     2461 gacgccctcg accagggcgg cggcgctgcg ccggtaaagg aacagcagct ccagcggcgt
     2521 gtacccgcca agatcgacat agggtatttt gaccaagggc tgcatcgggc gttttgcagt
     2581 cggcgcttcg cttcctatat tgagactatc atgaccgatc tcaaatggcg ctccacaact
     2641 attctttccg tccgcaaggg ctctaccgtg atcgtcgccg gcgacgggca ggtgacgctg
     2701 ggcaatacgg tgatgaaggc caatgcccgc aaggtccgcc gcctcggcaa aggcgacgtc
     2761 attgcaggtt ttgccggggc cacggccgac gcttttgccc tgttcgagcg gctggaagcc
     2821 aagctcgagc aacatccggg caatctggcg cgggcctgcg tcgagctggg caaagactgg
     2881 cgcaccgacc gctatctgcg gcggctggaa gccatgatgg cggtggccga cgccaccacc
     2941 tcgctggtcc tgtcgggcac cggcgacgtg gtggagccgg aggacgggct gatcggcatc
     3001 ggttccggcg ggccttacgc catggccgcg gcccgggcac tcttggcctt cgacctgccg
     3061 gccgaggaaa tcgcgcgccg ggcgatgaag atcgcggccg acatctgcat ctacaccaac
     3121 gagaacgtca ccatcgaaag cttgtccagt tgatcagttc cgccttcacc ccccgcgaga
     3181 tcgtttccga gctcgaccgt tacatcgtcg gccagcacga ggccaagcgc gccgtcgcca
     3241 tcgcgctgcg caaccgctgg cgccgccagc agctccccgc cgatctgcgc gaggaagtcc
     3301 tgcccaagaa cattctgatg atcggtccga ccggggtcgg aaagaccgaa atttcccgcc
     3361 ggctggccaa gctggcgcag gcgccattcc tgaaagtgga agcgaccaag ttcaccgaag
     3421 tcggctatgt cgggcgcgac gtcgagcaga tcgtgcgcga tctggtggaa gtcgccatcg
     3481 cccaggtgcg cgagaagaag cgccgcgacg tcgaggccca ggccgaggcg cgcgccgagg
     3541 atcgcgtgct ggacgcgctg gcgaaatctt ccggcggcgt ctcccgcgag tccttgcgca
     3601 aagcgttgcg cgcgggcgaa ttcgacggca aagaaatcga aatcgagatg aaagacgccg
     3661 gcggcatgcc ttctttcgag attccgggca tgccgaacgc gcagatttcc atggtcaatc
     3721 tgggcgacat gttcggcaaa gccttcggca gccgctccaa gccgcggcgg atgagcgtcg
     3781 ccgaagcgca tgcgccgctg ctcgccgaag aggccgacaa tctcctggat caggatgcga
     3841 tgctgcgcga agccatcgcg acggtggaag acaacggcat cgttttcctc gacgagatcg
     3901 acaagatctg cgcccgctcc gaattccgcg gcggcggcga tgtttcgcgg gagggcgtgc
     3961 agcgtgatct tttacctctc atcgaaggca ctaccgtcgc caccaagcac ggccaagtga
     4021 agaccgacca tatcctgttc atcgcctcgg gcgcgtttca caccgccaag ccctcggacc
     4081 tgttgccgga actgcaaggc cgcttgccga tccgcgtgga attgaaggcg ctgacgcgcg
     4141 acgatttcca gcgcatcctc accgaacccg aagccagcct gatcaaacaa tatgtcgccc
     4201 tgctcgccac cgaagaggtt gcgctcgact ttaccgcaga cggcatcggc gccatcgccg
     4261 acatcgccgc ccaggtgaac gccagcgtcg agaatatcgg cgcccgccgg ctgcaaaccg
     4321 tcatggagcg ggtgctcgac gagatcagct tctcggcggc cgagcgccag ggcgagaaac
     4381 tggtcatcga cggcgcttat gtgcgggcgc gggtggaaga ccttgccaaa gaccaggatt
     4441 tgtcccgctt cattctgtga aatctgcgcg ggcctgcggc gccgcgacgg caaaacatat
     4501 gtcaaaatct gcatatgata gttgaccccg ggatcattcc ctgcaaggag tcatctgttt
     4561 tccgagcgga ggggttgata tggcgcggtc gagctatctt ttcaccagcg aaagcgtgtc
     4621 cgaggggcac ccggacaagg tctgtgaccg gatctccgac gaagtcgtcg atctgttctt
     4681 ccgcgaaggc cccaaacacg gtttttcgcc ctgggatttg cgcgtggctt g
//
LOCUS       DASWPU010000557         5178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_17360,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000557 DASWPU010000000
VERSION     DASWPU010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17360"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /gene="msrA"
                     /locus_tag="VGC27_11680"
     CDS             complement(<1..456)
                     /gene="msrA"
                     /locus_tag="VGC27_11680"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY0283271.1"
                     /translation="MEKATFGAGCFWGVESFFRQVPGVLDAVCGYAGGHVDHPTYKQV
                     CTDETGHAEVVEVNFDPAKLSYGKLVEVFFANHDPTTLNRQGPDICTQYRSAIFTHSP
                     EQERVAREKKSALEASGKFKRPIVTTIEAASRFWPAEDDHQRYFEKNGLP"
     gene            585..1523
                     /locus_tag="VGC27_11685"
     CDS             585..1523
                     /locus_tag="VGC27_11685"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF009251.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY0283272.1"
                     /translation="MDVLKETHFTELGERKQGKVRDMYFQPEKLILVSTDRHSSFDRI
                     IAHIPNKGAVLNLSSAFWFRQTADIVPNHVLDVPDPNVTVARRLKPLPVEAVVRGYLT
                     GTTSTSIWTRYRDGERSFGGLTLPDGLKKNTKLAQPIFDPTTKEDTHDRALSLKELVD
                     EGFIGEELLLTLRKTALALFARGQEVAAKHGLLLVDTKYEFGVDEKGVLTLIDEIHTP
                     DSSRFWKLDSYEARLAKGEEPEYFDKEFLRIWFREHCDPYKDAVLPEAPADMVTELSR
                     RYIEIYEQLTGEKFAVDLSLPPVERIGKNLAQYRIR"
     gene            1565..2752
                     /gene="thiO"
                     /locus_tag="VGC27_11690"
     CDS             1565..2752
                     /gene="thiO"
                     /locus_tag="VGC27_11690"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEY0283273.1"
                     /translation="MKVVVIGAGVAGLGIGWKLAKAGASVTILERAQAGRGATTVSAG
                     MIAAAAELGHADTPEAAFAKRANELWPGFVTELEAESGIPVDYRQNGALMVALDSGGR
                     PSPPAQGADVAMLTAGQARAMEPMLRENIAGALWAPREAQIDTHALVRALVTAFAKAG
                     GTILANETAVRIETEGGAAGVGRAFGVRTPFALHHADAYVIAAGAWSSCLEGLPPEAV
                     PPVVPIKGEIVVLTPPVGAELPRHVVRGNGIYVVPRIGRLLVGATMERAGFDTGKTPE
                     ATRWLLGQATGLMPGLRGWEVAEHWAGLRPATPDGMPILGQTVVEGLYVASGQFRNGI
                     LFAPAIAEAMSRLILERVMETPAFDPRRFDGAKPQAPASAIETAHKAPGKETGFREWH
                     TGF"
     gene            2734..3720
                     /locus_tag="VGC27_11695"
     CDS             2734..3720
                     /locus_tag="VGC27_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEY0283274.1"
                     /translation="MAYWFLAGGLLLLIAGSEAAVRGGVGLARVMNLSPVLIGLLVIA
                     SATSAPELFVALRAATAGAPDIALGDIVGGNILMLLLALGLGALVRPLASPPKVVLRD
                     GGAMLAGALLVAFFALNGDLTRRDGLFLFLGFLAYLAGTFFSDWRRSADHSVALSRAL
                     ARSRREPPPVVGSLFLLILGLICLVLGALVTVNGSVALARALNLQQAFVGLTIVAFGT
                     SLPKLIAVLVAAVRGHTDVAVGLLIGAVVFNLLGVLGITAMVAPLAVSRMLASADIYV
                     MVAASAALLPLLAMRWRLSRPRGALLALSYLCYVAFLVWRQGLLPLSLLGIG"
     gene            3779..4180
                     /locus_tag="VGC27_11700"
     CDS             3779..4180
                     /locus_tag="VGC27_11700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY0283275.1"
                     /translation="MDFELDQRLDSASSFIADWPLSRVILMNDARFPWLVLVPRRPGA
                     TEVFDLNEEDRVTLIRECCRAGERLKKLTDCKKINICAIGNNVPQLHVHVIGRNEDDP
                     VWPSPVWSRGYAVPYEPAALAEFLARLINGL"
     gene            4230..5057
                     /locus_tag="VGC27_11705"
     CDS             4230..5057
                     /locus_tag="VGC27_11705"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0283276.1"
                     /translation="MLAEAVNHSSEKLTVVAALQRASAATGSDFHYLLGTAMRESSLK
                     PQAQSATSSAAGLFQFIEQTWLGVVKEFGAKHGLGSYAAAIGKGANGRFHVDNAADRQ
                     AILALRNNPQVSALMAGEYAQQTKTEMQNSLGREVCSGELYAAHFLGADAACRLIRMN
                     DTTPNATAASVFPQAAGANRSVFYRADGAAKTVREVYDWALNQPSAASQTPVATASAA
                     GPVSYGPDYSSLLASLWAPTAKGFFSSGEGGFPFLLTPGVLDILSSFSPVAKDRDGS"
BASE COUNT          853 a         1672 c         1685 g          968 t
ORIGIN      
        1 cggcaggccg ttcttctcga aatagcgctg gtgatcgtct tcggcgggcc agaagcgtga
       61 cgcggcttcg atcgtggtga cgatgggccg cttgaacttg ccggaggcct ccagcgccga
      121 cttcttctcg cgcgccacgc gctcctgttc gggcgagtgg gtgaagatgg ccgagcggta
      181 ctgcgtgcag atatccggtc cctggcggtt cagcgtcgtc ggatcgtgat tggcgaagaa
      241 cacctccacc agcttgccgt aggagagctt tgccggatcg aagttcactt ccacgacttc
      301 cgcgtgtccc gtctcgtcgg tgcagacctg cttgtaggtc ggatgatcga catggccgcc
      361 ggcatagccg cagacggcgt ccagcacgcc cggcacctgc cggaagaagg actcgacgcc
      421 ccagaaacac cctgcgccga atgtggcttt ttccattctc gttctccttg ccgcggccgc
      481 cagcgtagca gaaattcacg ccctgtcagg cactttgacc gggcttccca acggcaatac
      541 ggtggcgaga aacaatcccg attccaaccc ggaaaacgag ccgcatggac gttctcaaag
      601 aaacccattt caccgagctt ggcgagcgca agcagggcaa ggtgcgcgac atgtatttcc
      661 agccggagaa gctgatcctg gtctcgaccg accggcattc ctccttcgac cgcatcatcg
      721 cgcatattcc gaacaagggc gcggtcttga acctttcgag cgccttctgg ttccggcaga
      781 cggccgacat cgtgccgaac cacgtgctcg acgtgccgga cccgaacgtg accgtcgccc
      841 ggcgcctgaa gcccttgccg gtggaagcgg tggtgcgcgg ctatctgacc gggacgacgt
      901 cgacctcgat ctggacccgc taccgggacg gcgagcggtc gttcggcggc ctcacgcttc
      961 ccgacggcct gaagaagaat accaagctcg cgcagccgat cttcgacccg accaccaagg
     1021 aagacacgca cgaccgcgcc ctcagcctga aggagctggt ggacgaagga ttcatcggcg
     1081 aggagctgct gctgacgctg cggaagacgg cgctcgccct gttcgcgcgc ggacaggagg
     1141 tcgcggccaa gcacggtctt ctcctcgtcg ataccaaata cgagttcggc gtggacgaga
     1201 agggcgtgct cacgctgatc gacgaaatcc acaccccgga ctcgtcgcgc ttctggaaac
     1261 tcgattctta cgaggcgcgg ctggcgaagg gagaagagcc ggaatatttc gacaaggaat
     1321 tcctccgcat ctggttccgg gagcattgcg atccgtacaa ggacgcggtc ctgccggagg
     1381 cgcccgcgga tatggtgacc gaattgtcgc gtcgctacat cgagatctac gagcagctta
     1441 ctggagagaa attcgccgtc gatctgtcgc tgccgcccgt ggagcggatc ggcaaaaatc
     1501 tggcgcaata ccgcatccgc tgacgcgcgc ccggccgcgt gtgcgccgcc gcggggagat
     1561 cgacatgaag gtcgtcgtca ttggcgccgg cgtcgccggc cttggcatcg gatggaagct
     1621 ggcgaaggcc ggtgcgagcg tcacgatcct cgaacgggcg caagccggac gcggggcaac
     1681 gacggtctca gccggcatga tcgccgctgc cgccgaactc ggccacgccg atacgccgga
     1741 agcggctttc gccaagcgcg ccaacgagtt gtggcccggg tttgtcacgg agcttgaagc
     1801 ggaatccggc attccggtcg attatcggca gaacggcgcg ctgatggtcg cgctcgactc
     1861 cggcggccgg ccaagcccgc ctgcccaggg cgccgatgtc gccatgctga cggccgggca
     1921 ggcacgggcg atggaaccga tgttgaggga aaatatcgcc ggcgcgctgt gggcgccgcg
     1981 cgaagcgcag atcgacaccc acgcactggt ccgggcgctg gtaacggctt tcgcgaaggc
     2041 cggaggaacc atccttgcca acgaaaccgc cgttcgcatc gaaaccgaag gcggcgccgc
     2101 aggcgtgggc cgcgccttcg gggtgcgcac gccgttcgcg ctgcatcatg ccgacgctta
     2161 cgttattgcc gcgggtgcct ggagttcatg cttggaagga ttgccgccgg aggcggttcc
     2221 gccggtggtt ccgatcaagg gcgagatcgt cgtgctgacg ccgcccgtgg gcgccgaatt
     2281 gccccggcac gtcgtccggg gcaacggcat ctatgtcgtc ccgcggatcg ggcggctgct
     2341 ggtgggtgcg acgatggaaa gggccggctt cgacaccgga aaaacgccgg aggccacccg
     2401 ctggctgctc gggcaggcca ccggcctgat gcccggcctg agaggctggg aggtggccga
     2461 acattgggcc ggtctgaggc ccgcgacacc ggacggaatg ccgatcctgg gtcagaccgt
     2521 cgtggagggc ctttacgtag ccagcggcca attccgcaac ggtatcctgt ttgcaccggc
     2581 aatcgcggag gccatgtccc gtctcattct ggaacgggtc atggaaacgc ctgctttcga
     2641 ccctagacgg ttcgacggcg ccaagccgca ggcgccggcc tcggcgatcg aaacggcgca
     2701 taaggctccc ggaaaggaga ccgggtttcg ggaatggcat actggtttct agcgggcgga
     2761 cttctgttgc tgatcgcggg cagcgaagcg gccgtgcgcg gcggcgtcgg tctggcgcgg
     2821 gtgatgaatc tttcgcccgt tctgatcggc ctgttggtca tcgcaagcgc cacctccgca
     2881 cccgagctgt ttgtggcgct gcgcgcggcg acggccggcg cccccgacat tgcactcggc
     2941 gacatcgtcg gcggcaacat cttgatgctg ctgttggcgc tcggacttgg ggcgctggtc
     3001 cgaccattgg catcgccgcc gaaagtcgtg ctgcgcgacg gcggcgcgat gctggccggc
     3061 gcgcttctcg tcgccttctt tgccttgaac ggagacctta cgcggcgcga cgggttgttc
     3121 ctgttcctcg ggtttctcgc ctacctcgcc gggacgttct tcagcgactg gcgccgttcg
     3181 gcggatcact ccgtggcgct gtcgcgcgcc ctggcgcgct cgcgacgcga accgccgccc
     3241 gtggtcggca gcctgtttct tctgatcctg ggtttgatct gtctggtgct gggcgctctc
     3301 gttacggtga acggcagcgt ggcgctggcg cgcgcgctga acctgcaaca ggcctttgtc
     3361 ggcctaacca tcgtcgcctt cggcacctcg ctgccgaaat tgatcgccgt gctcgtggct
     3421 gcggttcgcg gccacaccga tgtcgccgtc gggcttctga tcggcgccgt cgtcttcaac
     3481 cttctgggcg ttctgggtat caccgccatg gtcgcgccgc tggccgtatc gcggatgctg
     3541 gcatcggcgg acatctatgt gatggtggca gcgagcgccg ctttgctacc gttgctggcg
     3601 atgcgttggc ggctgtcgcg gccgagaggc gctttgcttg cgctctcgta tctctgctat
     3661 gttgcgttct tggtatggcg tcagggattg ctcccgcttt ccctgctagg aattggttaa
     3721 cgcgcgttcc ggcgaaacat ccgccgcgga atcgcgcgaa tatccggaga agtgcattat
     3781 ggactttgaa ctcgaccagc gtctggattc cgcttcgtcc ttcattgcgg actggccatt
     3841 atcgcgcgtc atccttatga acgacgcgcg gttcccgtgg ctggtgctgg tgccgcgccg
     3901 ccccggtgcg accgaagttt tcgacttgaa cgaggaagat cgcgtaacgc tgatccggga
     3961 atgctgccgc gcgggcgagc ggctgaagaa gctgaccgac tgcaagaaga tcaacatctg
     4021 cgccatcggc aacaacgtgc cgcagcttca tgtccatgtc atcgggcgca acgaagacga
     4081 tccggtctgg ccgtccccgg tgtggagccg cggctacgcc gttccctacg aacccgccgc
     4141 gctggccgaa tttttagccc gtctcattaa cggtctttaa ccttattctc ctataggctg
     4201 gctgccgggt ccgtcgggag aacccgaaca tgctggcaga agccgtcaat cactcgagcg
     4261 agaagctcac ggtcgtggcc gctttgcagc gcgccagcgc cgccaccggc tcggatttcc
     4321 attacctgct gggcaccgcg atgcgggagt cgagcctgaa accgcaagcc caatcggcca
     4381 cctcgtcggc cgccggcctg tttcaattca tcgaacagac ctggctcggc gtggtcaagg
     4441 agttcggcgc gaaacacggt ctgggctctt acgcggccgc gatcggcaag ggcgccaacg
     4501 gacgctttca tgtggacaac gccgccgatc gtcaggccat tctcgccctg cgcaacaatc
     4561 cgcaagtttc ggccctcatg gccggcgaat atgcgcagca gaccaagacc gagatgcaaa
     4621 acagtttggg gcgcgaggtt tgcagcggcg aactctacgc cgcgcatttc ctgggggcgg
     4681 acgccgcctg ccgcctgatc cggatgaacg acaccacccc gaatgcaacc gcggcgagcg
     4741 tctttccgca ggccgcgggc gccaacagaa gcgtcttcta tcgtgccgac ggcgccgcca
     4801 agaccgtgcg cgaagtctat gattgggcgc tgaaccagcc ttccgccgcc tcgcaaacgc
     4861 ccgtcgcgac ggcctcggcg gccggcccgg tcagctatgg ccccgactac agcagcctgc
     4921 tcgcctcgct gtgggcgcca accgccaaag ggtttttctc cagcggcgag ggcggtttcc
     4981 cgttcctcct gacgcccggc gtgttggaca ttttgtcgtc cttttcaccg gttgcgaagg
     5041 accgcgacgg ctcgtaaggc ttcccctctt ttggggggaa ccattgcgcg cggcgttgct
     5101 tgccatctgg gcggtgatcc tgtcggtcat cttcatgcag accgcccacg gcctgcaaac
     5161 cgacctcatc ggcgtgcg
//
LOCUS       DASWPU010000558         7947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_36014,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000558 DASWPU010000000
VERSION     DASWPU010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36014"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VGC27_11710"
     CDS             complement(<1..510)
                     /locus_tag="VGC27_11710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0283277.1"
                     /translation="MAKPKIAIIISTTRAARFGDKPAKWIADIAKQRDDMSVELVDLR
                     DYPMPFFDEVASNAWAPSKNEVAQRWQKKVAEFDGYIFVTAEYNRGISGVLKNAIDYA
                     YPEWVKKPAAYVGYGSVGGARAIEQLRLINVELQMAPMRTGVHIQGADFMAVWQQGKD
                     IAELTYLEQN"
     gene            complement(539..1237)
                     /locus_tag="VGC27_11715"
     CDS             complement(539..1237)
                     /locus_tag="VGC27_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0283278.1"
                     /translation="MIDVRPFNQLGGADHGWLKAKHHFSFAEYYEPKRMQWGNLRVWN
                     DDEIAPHSGFPPHPHANMEIVTYVREGAITHEDSMGNKGRTAAGDVQVMSAGTGVKHS
                     EFNLENETTRLFQIWILPTQPGGAPSWGAKPFPKSDRSGRFVALASGFAEDKDALPIR
                     TEARVLGATIKAGERVTLALDPGRHVYLVPAKGVIEVNGVRVNARDGAAVRDENKIEI
                     AVLKDAEIVLVETA"
     gene            1353..2285
                     /locus_tag="VGC27_11720"
     CDS             1353..2285
                     /locus_tag="VGC27_11720"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0283279.1"
                     /translation="MRLPDLEAWGIFAKVASTGSFAKAADELVLSHATVSKAISRLEG
                     RLGERLFHRSSRRLSLTETGRVLATRAARILSEGEDAEAEAQACSVSPRGKLRLAAPM
                     SFGLDQIAPILPQFLAAYPEVSIDLQLDDRVVDMIGAGIDVAVRIAALPDSSLMVRRL
                     CPVERFVVGAPAYFEQHGRPARPGELKDHACLGYTYLATGNVWHFANPAGEEESVAVT
                     GPLSANSGDALEPALLAGLGVALQPDFVVWQALASGKLERVLRDWSAPPLNVNVLTPA
                     GGPRALRVTVLIEFLVRHFSADSVPWATLHKDCS"
     gene            complement(2267..3523)
                     /gene="hydF"
                     /locus_tag="VGC27_11725"
     CDS             complement(2267..3523)
                     /gene="hydF"
                     /locus_tag="VGC27_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HEY0283280.1"
                     /translation="MSQNSTVSALKASRLHIGIAGRTNVGKSSFLNMVAGQDVAITSA
                     EPGTTTDVVEKTMELLPIGPVVFLDTAGMDDVSALSAARIEKAKKALRRADILTLIVT
                     PDAWSDHEDALVNEARAASIPVLVVVNKADLSEPSPDFLSLLDVKRLPWIQGSATGHE
                     AEQETYRQAFKDNIRQLLPRLFSAPPALLGDLLPPGGMAVLVVPIDLGAPQGRLIVPQ
                     VQSLRDLLDNDASALVVKDREYAATLARLAKAPDLVVCDSQVVARVVADTPPSVPLTT
                     FSILFARFKGDLTELARGAGVLNRLKQGDKILVAEGCSHHPLEDDIGRVKIPRWLRQF
                     AGCELDIATCAGHDFPPDLKDYRLVIHCGGCVMTRQEMLSRLTEAQSEAVAMTNYGLA
                     ISVLQGVLHRALSPFPDALAAYEQSL"
     gene            complement(3529..4953)
                     /locus_tag="VGC27_11730"
     CDS             complement(3529..4953)
                     /locus_tag="VGC27_11730"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEY0283281.1"
                     /translation="MKLDGHPKSPGPVRKERDALGEIEIAADALWGIHTERALHNFRF
                     SERRVPTALIGAIATVKHAAAAANAELGYLDKGIADAIVAACAEVCEGGAAMEAQFPL
                     DALQGGAGTSTNMNVNEVVANLALKRLGKAAGDYDSVHPLHHVNLHQSTNDVYPTALR
                     IAAIAGVRRLSGAMAKLQGAFQAKERAFQGIAKMGRTELQAAVPMTLGQEFSAFAEAI
                     ARDRWRTFKCEERLRLVNLGGTAIGTGITAPRRYIFRVIEVLRTLTGMGLSRNENVID
                     ATANADAFVEVSGILKASAANLSKIAGDLRLLHFLGEIALPPLQAGSSIMPGKVNPVM
                     MEAVMQAGMAVRANDAVVTECAAHGTLQINEFMPLLASALLESIAVLEAAALAFAGHV
                     EGIEADPERCAEHLDDSPTLFTAFLPRIGYERADALVKEYAVAKAEDSSLTLRRFLSE
                     RLDAAMVNEVLSAPNLLKLGHTDS"
     gene            complement(4931..6064)
                     /gene="hydE"
                     /locus_tag="VGC27_11735"
     CDS             complement(4931..6064)
                     /gene="hydE"
                     /locus_tag="VGC27_11735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HEY0283282.1"
                     /translation="MTDFARLTAQETFSRNDLLAILSASAGSEIDLVRRAAEAVLLEQ
                     CGSRVYLRGLVETSNACACDCLYCGIRKSNKSVNRYTLSVDQIAACARQCAELGYGSM
                     VIQSGERSDKKFIDLTLAALRRIKHETRSATQPEGLGITLCIGQQSRETYQRLFDAGA
                     HRYLLRIETTNPDLFARIHPEDQTLESRLECLALLREIGYQVGTGVMIGLPGQTLDDL
                     AGDIMFFAKHDIDMIGMGPFIPDPATPLGDESCPGVKDRIRLSLLMIAATRLKLRDVN
                     IASTTALQTLDPIGREKGLRFGANVIMPQVTPPEVRKDYQLYPGKPCLDENALECHSC
                     LGARIAFAGRTIGYNTWGDSAHAVRRNAGGRNGVVPDEARRAS"
     gene            complement(6071..>7947)
                     /locus_tag="VGC27_11740"
     CDS             complement(6071..>7947)
                     /locus_tag="VGC27_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HEY0283283.1"
                     /translation="LKVSPGTTVLEAAVKAGIHIPTLCHHDDLCTEALFSQTTSHAHQ
                     DVHDPHKHAESGMLPTPSSCRLCLVEVEGLSKLHASCALPVYSPIKVHTTSTTIRHAR
                     RDILELMMSEHYGDCFACARNGTCELQKLCADYGVTSERFGRPHEPLFEMADPNNIIQ
                     RDMNKCILCRRCVRMCALYQGVYALTTEGRGDHSRISTFMGREMMDAWCINCGQCINR
                     CPTGALAERDDTEAVWRAIEDPDKIVVIQTAPAPRASIGESFGMEAGTPMTFQMNTAL
                     KRCGFDKVFDTNFSADLTILEEGAELISRLYKAVVLKDNNVALPQFTSCSPGWIKFIE
                     HFYPNYLPNLSSAKSPQQMMGAVIKTYFAEKNGWDPSKIVSVSLMPCTAKKFESGRPE
                     MDASGYRDVDYALTTREVARMFKQAGIDLPKLDKSPFDDPFGIPTGSGVIFAATGGVM
                     EAALRTVIEVVTGDKVERLFDHADIKPVRGFEGVRMAEITIDKVGPVPAILSHLVPDF
                     EWLRGVTVKVAMAHGTANAIRVMQDIAEGGTLSQCHFIEFMACPGGCLGGGGQPIPTS
                     AEIRAARAKAIYGEDADYVVRKSHENEDVLRIYNEFLKEGPCSHKSHELLHTHYTERV
                     "
BASE COUNT         1403 a         2511 c         2514 g         1519 t
ORIGIN      
        1 gttctgttcc aaataggtca gctcggcgat gtccttgcct tgctgccaca ccgccatgaa
       61 atcggcgccc tggatgtgaa cgccggtccg catcggggcc atttgcagtt cgacgttgat
      121 caaccgcaac tgctcgatgg cgcgcgcacc gccgaccgag ccatagccga cataagcggc
      181 gggtttcttg acccattctg ggtaggcgta atcgatggcg ttcttgagaa caccggaaat
      241 gccgcgatta tattcggccg tcacgaagat gtagccgtcg aattccgcca ccttcttctg
      301 ccagcgctgc gccacctcgt ttttggaagg cgcccaggcg ttcgaggcca cttcgtcgaa
      361 aaagggcatg ggatagtcgc gcagatcgac aagttccacg gacatatcgt ccctttgctt
      421 ggcgatgtcg gcaatccatt tcgccggctt gtcgccgaaa cgggccgcgc gggtcgtgct
      481 gatgatgatt gcgattttcg gctttgccat ttgaagctcc aatggttgcg attaggggtt
      541 atgcggtctc gaccaagacg atttcggcgt ccttaagaac ggcaatctcg attttgtttt
      601 cgtcgcggac ggcagcgccg tcgcgggcgt tcacgcgcac gccgttcact tcgatcacgc
      661 ccttggcggg caccagatag acatggcggc ccggatcgag agcgagcgtc acgcgttcgc
      721 cggccttgat tgtggcgccc agcacccgcg cctcggtgcg gatgggcaag gcgtccttgt
      781 cctcggcaaa accgctggcc agcgcgacga agcggcccga acggtcggat ttcgggaacg
      841 gtttcgcccc ccacgacgga gcgccgcccg gctgcgtcgg cagaatccag atctggaaga
      901 gccgggtggt ttcgttctcc agattgaatt ccgaatgttt gacgccggtg ccagccgaca
      961 tcacctgcac gtcgccggct gcggtgcggc ccttgttgcc catcgaatcc tcgtgggtga
     1021 tggcgccctc gcggacatag gtgacgatct ccatgttggc gtggggatgg ggcggaaaac
     1081 ctgaatgcgg cgcgatctcg tcgtcgttcc agactcgcag attgccccac tgcatgcgct
     1141 tcggctcgta atattcggcg aaggagaagt gatgcttggc cttgagccag ccatggtcgg
     1201 ccccgccgag ctggttgaac ggtctgacat cgatcatcgg attgtctcct tgaaactgcg
     1261 tcgcactctt tcaaggacga acatggctat ggatttgtac ctctcaaata gaaataatag
     1321 aatatcatcg tttcaaaaaa tgaggttcca cggtgcggct acccgatctc gaggcctggg
     1381 gcatctttgc caaggtcgcc tcaaccggct ccttcgccaa ggccgccgac gagctggtgc
     1441 tctcccatgc gactgtttcc aaagcgattt cccgcctgga gggccgcctc ggcgagcgcc
     1501 tgttccaccg ctcctcgcgc cgcctgtcac tcaccgagac cggccgggtc ctggcaacgc
     1561 gcgccgcacg tatcttatcc gaaggtgaag acgccgaggc cgaagcccag gcctgctccg
     1621 tctcgccgcg cggcaagctc cggctcgcgg ccccgatgtc gttcggactg gatcagatcg
     1681 cgccgatcct gcctcagttc ctcgcggcct atccggaggt gtcgatcgac ctccagctcg
     1741 atgaccgcgt ggtcgacatg atcggcgccg gcatcgatgt cgcggtccgc atcgcggcct
     1801 tgccggattc gagcctcatg gtgcgcaggc tttgcccggt cgaacggttc gtggtcggag
     1861 caccggctta tttcgagcag cacggacgcc cggcgcggcc gggcgagctg aaggaccacg
     1921 cctgtctcgg ctacacctat ctggcgaccg gcaatgtctg gcatttcgcc aaccccgccg
     1981 gagaagaaga atccgttgcg gtgacgggcc cgctcagcgc caacagcggc gatgcgctgg
     2041 agccggcgct gctggcggga ctgggcgtgg cgctgcaacc ggacttcgtc gtctggcaag
     2101 cgctcgcaag cggcaaactc gaacgcgtgc tgcgcgactg gtccgcgccg ccgctgaacg
     2161 tcaacgttct gaccccggcg ggcggaccgc gcgcgctgcg ggtgacggtg ctgatcgaat
     2221 tcctcgtccg gcatttttcg gcggactcgg tgccttgggc gacgcttcac aaggattgtt
     2281 cgtaagccgc caaggcatcg ggaaacggcg acagcgcgcg atgcagcacg ccttgcagca
     2341 ccgaaatcgc caagccgtaa ttggtcatcg ccacggcttc gctttgggct tcggtcaggc
     2401 gcgagagcat ttcctggcgc gtcatcacgc agccgccgca atggatgacc agacgataat
     2461 ccttcaagtc cgggggaaaa tcatgaccgg cgcaggtggc gatatctaat tcgcagccgg
     2521 cgaattggcg cagccagcgc ggaatcttca cccgcccgat atcgtcttcc agcggatggt
     2581 gggaacagcc ttcggcgacc aggatcttgt cgccttgctt gagacggtta agcacccccg
     2641 caccgcgcgc caactcggtc aggtcgcctt tgaagcgcgc aaagagaatg gagaacgtcg
     2701 tcagcggcac cgacggcggc gtatcggcga cgacgcgcgc caccacctgc gaatcgcaaa
     2761 ccacgagatc gggcgctttg gcgagccgcg ccagggtggc ggcatattcc cggtccttca
     2821 ccaccagcgc gctggcgtcg ttgtcgagca gatcgcgcag gctctgcact tgcggcacga
     2881 tcagccgacc ctgcggcgcc cccaaatcga tcggcaccac cagcaccgcc atgccgcccg
     2941 gcggcagcaa gtcgcccagc aaggccggcg gcgcgctgaa caggcgcggc aagagctggc
     3001 gaatattgtc cttgaacgcc tggcgatagg tctcctgctc cgcttcatgg ccggtggcgc
     3061 tgccttgaat ccagggcagc cttttcacgt ccagcagcga caggaaatcc ggcgacggtt
     3121 cgctgagatc ggccttgttg accacgacca gaaccgggat ggaagccgcg cgcgcttcgt
     3181 tcaccagcgc gtcttcgtga tcgctccatg cgtcgggcgt gacgatgaga gtcagaatgt
     3241 cggcgcgccg cagcgccttc ttggccttct cgatgcgcgc cgcggacagc gccgatacgt
     3301 cgtccatgcc ggcggtgtcg aggaacacca ccgggccgat cggcagcaat tccatcgtct
     3361 tctcgaccac atccgtcgtc gtgcccggct cggcggaggt gatggcgaca tcctgacccg
     3421 ccaccatgtt caggaacgaa gacttgccca cgttggtgcg cccggcgatg ccgatgtgaa
     3481 ggcggctggc tttcaatgcc gatacggtgg aattctgcga catacggctt agctatccgt
     3541 gtgaccgagc ttgagaagat tgggggccga aagcacttcg ttcaccatgg ccgcatcaag
     3601 ccgctccgac aggaagcggc gaagcgtcaa ggaagaatcc tcggctttgg cgacggcgta
     3661 ttccttcacc agcgcatcgg cgcgctcata gccgatccgg ggcaggaagg cggtgaacaa
     3721 ggtcgggctg tcgtccagat gctcggcgca gcgctccggg tctgcctcga tgccttcgac
     3781 atgaccggca aacgccaaag cggcggcttc caggacagcg atggattcga gcagcgctga
     3841 ggccagcagc ggcatgaatt cgttgatctg caacgtgccg tgtgcggcgc attcggtgac
     3901 gacggcgtcg ttggcgcgca ccgccatgcc cgcctgcata accgcctcca tcatcaccgg
     3961 attgaccttt cccggcatga tcgaggaacc cgcctggagc ggcggcaggg cgatttcgcc
     4021 gaggaaatgc agcagccgca gatcgcccgc gatcttgctt aagttggcgg cggaggcctt
     4081 caggatgccg gagacttcga cgaacgcatc ggcattcgcc gtggcgtcga tcacgttttc
     4141 attgcgcgaa agccccatgc cggtgagcgt gcgcagcacc tcaatgacgc ggaagatgta
     4201 gcggcgcggc gcggtgatcc cggtgccgat ggcggtgccg ccgagattga ccaggcgcag
     4261 ccgctcttcg catttgaagg tgcgccagcg gtcgcgcgcg atggcttcgg caaaggcgga
     4321 aaattcctgg cccagcgtca tcggcaccgc ggcttgcagc tcggtgcggc ccattttcgc
     4381 gatgccctgg aacgcccgct ccttggcctg gaaagcgcct tgcagcttcg ccatggcgcc
     4441 gctgagccgc cgcacccccg caatcgccgc gatccgcagc gccgtcggat acacgtcgtt
     4501 ggtcgattga tgcaagttga cgtgatgcag cggatggacg ctgtcgtaat cacccgcggc
     4561 ttttcccaga cgcttcaacg ccagattggc gaccacctcg ttgacgttca tgttggtcga
     4621 ggtgccggcg ccaccctgga gcgcatcgag cggaaactgc gcctccatcg ccgcgccgcc
     4681 ttcgcacacc tcggcgcaag cggcgacgat ggcgtcggcg atgcccttat cgagatagcc
     4741 aagctcggca ttggccgccg ccgctgcgtg cttgaccgtg gcgatggcgc cgatcagcgc
     4801 agtaggcacg cgccgttcgg agaatctgaa attatgcagc gcgcgctcgg tatggatgcc
     4861 ccaaagcgca tccgccgcta tttcgatttc tcccagggca tcgcgttcct ttcgcaccgg
     4921 acctggcgat ttaggatgcc cgtcgagctt catccggcac tactccgttc cttccgccgg
     4981 cgttgcgcct gacggcatgc gcggaatcgc cccaggtatt atagccgatc gtccgcccgg
     5041 caaaggcaat gcgcgcgccc aggcaggagt ggcattccaa ggcgttttcg tccagacagg
     5101 gttttcccgg atagagctgg taatccttgc gcacctccgg cggcgtgacc tgcggcatga
     5161 tcacattggc gccgaagcgc aggcccttct cgcgcccgat cggatcgagg gtttgcaagg
     5221 ccgtggtcga ggcgatgttg acgtcgcgca gcttgagacg cgtggcggcg atcatcagca
     5281 gactgaggcg gatgcgatcc ttgacgccgg ggcagctttc gtcgcccagc ggcgtcgccg
     5341 gatcgggaat gaacggaccc atgccgatca tgtcgatgtc gtgcttggcg aaaaacatga
     5401 tgtcgccggc gagatcgtcc agcgtctgtc cgggcaagcc gatcatgacg ccggtgccga
     5461 cctgatagcc gatctcccgc aacagggcga ggcattccag gcgcgactca agcgtttgat
     5521 cttcgggatg gattctggcg aacaggtccg gattggtggt ttcgatgcgc aacaggtagc
     5581 ggtgcgcgcc ggcatcgaac aggcgctggt aggtctcgcg cgactgctgg ccgatgcaaa
     5641 gggtgatgcc caagccttcc ggctgggttg cgctgcgcgt ttcatgctta atccggcgca
     5701 gggccgcgag cgtcaggtcg atgaatttct tgtcgctgcg ttcgcccgac tggatcacca
     5761 tcgagccgta gcccagctcc gcgcactgcc gcgcgcaggc cgcgatctga tcgacgctga
     5821 gcgtataacg gttaacgctc ttgttggact tgcggatgcc gcaatagaga caatcacagg
     5881 cacaggcgtt ggacgtttca accaggcccc gcagatagac ccgcgacccg cattgttcca
     5941 gaagtacggc ttcggcggcg cgccggacaa gatcgatctc actgccggca ctcgcgctca
     6001 ggatcgccaa caaatcgttg cggctgaacg tttcttgggc cgtcaggcga gcaaaatccg
     6061 tcaacgcaac ctataccctt tccgtgtaat gcgtgtgcaa aagctcgtgg ctcttgtggc
     6121 tacacggccc ttctttaagg aactcgttat agatgcgcaa tacatcctca ttttcgtggc
     6181 tcttgcgcac aacgtaatcg gcgtcctcgc catagatggc tttggcccgc gcggcgcgaa
     6241 tttccgctga tgtcggaatg ggttggccgc cgccgccgag gcagccgccc gggcaagcca
     6301 tgaattcgat gaaatgacac tggctgagcg tgccgccttc cgcgatgtcc tgcatgacgc
     6361 ggatcgcgtt ggcggtgccg tgggccatcg cgactttcac cgtcacgccg cgcagccatt
     6421 cgaaatcggg caccagatgg gacagaatcg cgggaaccgg cccgaccttg tcgatcgtga
     6481 tctcggccat gcgcacgccc tcgaagccgc gcaccggctt gatgtcggcg tggtcgaaga
     6541 gcctctccac cttgtcgccg gtcaccacct cgatcaccgt gcgcagcgcc gcctccatca
     6601 cgccgccggt ggccgcgaag atgacgcccg aaccggtcgg aatgccgaac ggatcgtcga
     6661 acggcgactt gtcgagcttc ggcaagtcga tgcccgcctg cttgaacatg cgggcgactt
     6721 cgcgcgtggt cagcgcatag tcgacgtcgc ggtacccgct ggcgtccatc tccgggcgcc
     6781 ccgactcgaa cttcttcgcc gtgcaaggca tcaaggagac gctgacgatc ttcgacggat
     6841 cccagccgtt cttctccgcg aaataggtct tgatcacggc gcccatcatc tgctgcgggc
     6901 tcttggccga ggaaaggttc ggcagataat tcggatagaa atgctcgatg aacttgatcc
     6961 aaccgggcga gcaactggtg aattgcggca gcgccacgtt gttgtccttg agcaccaccg
     7021 ccttgtagag gcgcgagatc agctcggcgc cctcttccag aatcgtcagg tcggcggaga
     7081 aattggtatc gaagaccttg tcgaagccgc agcgcttcag ggccgtgttc atctggaacg
     7141 tcatcggcgt gccggcctcc atgccgaagc tctcgccgat ggaggcgcgc ggcgccgggg
     7201 ccgtctggat caccacgatc ttgtcgggat cctcgatggc gcgccacacc gcttcggtgt
     7261 cgtcgcgctc ggccagcgcg ccggtggggc agcggttgat gcactggccg caattgatgc
     7321 accaggcgtc catcatctcg cggcccatga aggtggaaat gcggctatgg tcgccgcgtc
     7381 cttccgtggt cagcgcatag acgccttgat agagcgcgca catgcgcacg cagcgccggc
     7441 acaggatgca cttgttcatg tcgcgctgga tgatgttgtt ggggtccgcc atctcgaaaa
     7501 gcggctcgtg cggccggccg aagcgctcgg aagtcacgcc gtaatcggca cacagcttct
     7561 gcaattcgca ggtgccgttg cgggcgcagg cgaaacagtc gccgtaatgc tcggacatca
     7621 tcagttcgag gatgtcgcgc cgcgcatgac ggatggtggt ggacgtcgtg tgcaccttga
     7681 tcgggctgta gaccggcagc gcgcaggagg cgtggagctt ggacagaccc tccacctcga
     7741 cgaggcagag ccgacagctc gaaggcgtgg gcagcatgcc gctttcggcg tgcttgtggg
     7801 gatcgtgaac gtcctgatgg gcatgcgacg tcgtttggga gaacagcgcc tccgtacaca
     7861 gatcgtcgtg atggcaaaga gtcgggatat ggatgcccgc cttgacggca gcttccagca
     7921 ccgtggtccc cgggctgact ttcagtt
//
LOCUS       DASWPU010000559         4126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_10431,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000559 DASWPU010000000
VERSION     DASWPU010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10431"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /gene="pnp"
                     /locus_tag="VGC27_11745"
     CDS             <1..1043
                     /gene="pnp"
                     /locus_tag="VGC27_11745"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY0283284.1"
                     /translation="KESFLLHYNFPPYSVGETGRMGGTKRREIGHGKLAWRAIHPVLP
                     SKLDFPYTIRVVSEITESNGSSSMASVCGASLSLMDAGVPITRPIAGIAMGLILEGDR
                     FAVLTDILGDEDHLGDMDFKVAGTDQGVTSLQMDIKIAGITEEIMRVALGQAKDGRLH
                     ILGEMNKALSHGRDQLGEYAPRIEQIKIPTDKIREVIGTGGKVIREIVEKTGAKINIE
                     DDGTVMVASADGESIRAALKWIKSIVAEPEIGEIYDGKVVKVMDFGAFVNFFGPRDGL
                     VHISELAPKRVAKTTDVVQEGQVVKVKLLGFDDRGKVRLSMKVVDQVTGEDLTKKQAA
                     EAQAVGEVQPGH"
     gene            1250..1711
                     /locus_tag="VGC27_11750"
     CDS             1250..1711
                     /locus_tag="VGC27_11750"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0283285.1"
                     /translation="MPKIDLNSVPERKGSFYPAPFHEKAAGRIKQALGDAGGLSQFGV
                     NLVHLPPGNWSSQRHWHSAEDEFVTVLSGELVLVTDAGEEVLRAGDCAAFPRNVANGH
                     HLINRSAETAVYLEVGTRSEDDFCSYPDIDLQIDCKIDRYAHKDGTFYPQR"
     gene            1841..2140
                     /locus_tag="VGC27_11755"
     CDS             1841..2140
                     /locus_tag="VGC27_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283286.1"
                     /translation="MRFISLVVVVIALMLLGADLVTSLENLDKGGHITVRSLATIWGL
                     LDQGSLDGFRHWTGQLPGFVAAAIDWLLGIYGWFAVGIIGMAMAFFFGRSKREDA"
     gene            complement(2191..2760)
                     /locus_tag="VGC27_11760"
     CDS             complement(2191..2760)
                     /locus_tag="VGC27_11760"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY0283287.1"
                     /translation="MVRLTRIYTKTGDKGLTSLGDGKRIAKHALRVLAFGAVDEANAA
                     VGLARLHAKGKKDAMLARIQNDLFDLGADLCVPEAVESKRSRLRIAEAQVERLEREID
                     AMNARLKPLESFVLPGGSALAAHLHLARAIVRRAERLMVELAQSEKVGEAALRYINRL
                     SDHLFVMSRAANKGRDVLWEPGATRAKSE"
     gene            2955..3122
                     /locus_tag="VGC27_11765"
     CDS             2955..3122
                     /locus_tag="VGC27_11765"
                     /inference="COORDINATES: protein motif:HMM:NF021772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY0283288.1"
                     /translation="MPVDAPEIVEVETTRIACDGGGVLGHPKVYLEMGDEDFVECPYC
                     DRRFVLKVETH"
     gene            3119..>4126
                     /locus_tag="VGC27_11770"
     CDS             3119..>4126
                     /locus_tag="VGC27_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY0283289.1"
                     /translation="MSAPTPLKKGDHLYLIDGSGYLFRAYHALPPLSRKSDGLPTGAV
                     SGYCNMLWKLLEDMKGDDAPSHLAVIFDAGKKTFRNEIYPEYKANRPEPPEDLIPQFP
                     LVRDATRAFGVACVEEPGFEADDLIATYARLAREAGARVTIVSSDKDLMQLVEDGKVR
                     LLDTMKNKKIGSAEVVERFGVPPSKVVEVQALAGDSSDNVPGVPGIGVKTAAELINTY
                     GDLETLLARAGEIKQPKRRQTLLENADKALLSQRLVLLDDHAPLRQEPGLFAVREPNP
                     AKLLAFLQAMEFASLAKRATAHYGIEDTDALPLPKEDAGEAGQQKATPRPASPKAAPD
                     DP"
BASE COUNT          785 a         1294 c         1293 g          754 t
ORIGIN      
        1 acaaagagtc attcctgctg cattataact tccctcccta ctcggtcggc gagaccggcc
       61 gcatgggcgg caccaagcgc cgcgaaatcg gccacggcaa actggcctgg cgcgcgatcc
      121 atccggtgct gccgtcgaag ctcgacttcc cctataccat tcgcgtggtg tcggagatca
      181 ccgagtcgaa cggctcttcc tcgatggcgt cggtctgcgg cgcctcgctg tcgctgatgg
      241 atgcgggcgt gccgatcacc cgcccgatcg ccggcatcgc catgggcctg atcctggaag
      301 gcgaccgctt cgcggtgctc accgacatcc tgggcgacga ggaccatcta ggcgacatgg
      361 acttcaaggt ggcgggcacc gaccaaggcg tcacctcgct gcagatggac atcaagatcg
      421 ccggcatcac cgaagagatc atgcgcgtgg cgcttggtca ggcgaaggac ggccgtctgc
      481 atattctggg cgagatgaac aaggcgctca gccacggccg cgaccagctg ggcgaatacg
      541 ccccacgcat cgagcagatc aagattccga ccgacaagat ccgcgaagtg atcggcacgg
      601 gcggcaaggt gatccgcgag atcgtcgaaa agaccggcgc caagatcaac atcgaggacg
      661 acggcacggt gatggtggcg tccgccgacg gcgaatccat ccgcgccgcg ctcaaatgga
      721 tcaagtccat cgtggccgag ccggaaatcg gcgagatcta cgacggcaag gtcgtcaagg
      781 tgatggattt cggcgctttc gtgaatttct tcggtccgcg cgacggcctg gtccacattt
      841 cggaactggc gcccaagcgc gtggccaaga ccaccgacgt ggtgcaggaa ggccaggtcg
      901 tgaaggtgaa gctgctcggc ttcgacgatc gcggcaaggt gcgcctgtcg atgaaggtgg
      961 tcgatcaggt caccggcgaa gacctcacca agaagcaggc ggccgaggcc caagcggtcg
     1021 gcgaagtcca gccgggtcat tgaacgattg aatcgtgtac agaccagccg cccgcggaac
     1081 gcatggattc cgcgggcggt tttgttttag aacgtgatcc cgtctagctg aattgctccg
     1141 gccgcccttc gacagtcggt agagacagcg taccgaccag catgagtgtg gcgcaatcct
     1201 atgtgggtgg atcgcgcttt agcttgacga aggccaaaga ggattgctca tgcccaagat
     1261 cgatttgaac tccgtgcccg agcgcaaagg ttctttctat cccgcgccgt tccacgaaaa
     1321 ggcggccggc cgcatcaagc aggctttagg cgatgccggc ggcctcagcc agttcggcgt
     1381 caatctggtt catctgccgc cgggcaattg gtccagccag cggcactggc attccgccga
     1441 agacgaattc gtcaccgtgc tgtccggaga gctggtgctg gtgacggacg cgggcgaaga
     1501 agtcctgcgc gccggggatt gcgcggcctt tccccggaac gtggcgaacg gccatcacct
     1561 gatcaaccga tctgccgaga ccgcggtcta tctggaagtc ggcacccgct cggaagacga
     1621 tttctgctcc tatcccgaca tcgacctgca gatcgactgc aagatcgacc gctatgcgca
     1681 caaggacggc accttctatc cgcaaagata aatacgccgc acaccgcccg gaatgttggt
     1741 taacgggaat tcatattatt gtcagccaag ggtgcgatgg ttagcctggc agcgggggaa
     1801 attcccatct ggaacggtcg ggggcggcat gcttggcttt atgcgcttta tcagtctggt
     1861 cgtggtcgtg atcgccttga tgctgctcgg tgcggatctg gtgacatcct tggagaatct
     1921 ggacaagggc ggccacatca ccgtgcgctc gctggcgacg atctggggct tgttggatca
     1981 aggcagcctg gacgggttcc ggcactggac cggccagctt ccgggctttg tcgccgccgc
     2041 gatcgattgg ctgctcggca tctatggctg gttcgccgtc ggcatcatcg gcatggcgat
     2101 ggccttcttc ttcggccgct ccaagcgcga agacgcatag aaaaacgagt agcgaatagg
     2161 gagtagcgaa ttgtttctac tcgccattcg ctactcgctt ttcgcccttg tggctcccgg
     2221 ttcccacagt acgtcgcggc ccttgttcgc cgcccggctc atcacgaaca gatggtcgga
     2281 cagccggttg atatagcgca gcgccgcttc gcccactttt tcgctttgcg ccagttccac
     2341 catcagccgt tcggcgcggc gtacgatggc gcgcgccaga tgcagatgcg ccgccagcgc
     2401 gcttcccccc ggcaggacga aggattcaag cggcttcagc cgcgcgttca tggcgtcgat
     2461 ctcgcgctcc agccgctcca cctgagcctc ggcgatgcgc agccgcgatc ttttgctctc
     2521 gaccgcttcc ggcacgcaga gatcggcgcc caggtcgaac agatcgttct ggatgcgcgc
     2581 cagcatggcg tcctttttgc ccttggcgtg gagccgcgcc agcccgacgg cggcattggc
     2641 ctcgtccacc gcgccgaagg ccagcacgcg caaggcatgt ttggcaatgc gcttgccgtc
     2701 gcccagcgag gttaagccct tgtcgccggt cttggtgtaa atgcgggtca gccgaaccat
     2761 gggacggaac atagggcacg gaaggagaat atggaatctc cctcatcctg aggagccctt
     2821 tcgccccctc cttcgagacg gcgcttcgcg cctcctcagg atgagggggc gaaagggcgt
     2881 cccgaaggac ggcaaaggtt gcagtggcgg gcgcggcagc tatcatccgg cttcctgtca
     2941 tcgagagtga cgccatgccc gttgacgcgc cggaaatcgt cgaagtggaa accaccagga
     3001 tcgcctgcga cggcggcggc gtcctcggcc atcccaaggt ctatctggaa atgggcgacg
     3061 aggatttcgt ggaatgcccc tattgcgacc gccgcttcgt attgaaggtt gaaacgcatt
     3121 gagcgccccc acgccgctta agaagggcga ccatctctat ctgatcgacg gatcgggcta
     3181 cctgtttcgc gcctatcacg ccctgccgcc cttgtcgcgc aagtccgacg gtctgcccac
     3241 gggcgcggtt tccggctact gcaacatgct gtggaagctg ctcgaagaca tgaagggcga
     3301 cgatgcgccg agccatctgg cggtgatctt cgacgccggc aagaaaacct tccgcaacga
     3361 gatctatccc gaatacaagg ccaaccggcc cgagccgccg gaggatttga tcccgcaatt
     3421 tcccctggtg cgcgacgcca cccgcgcctt cggcgtcgcc tgcgtggaag agccgggctt
     3481 cgaggccgac gatctgatcg ccacctatgc gagactcgcg cgcgaggccg gggcgcgcgt
     3541 caccatcgtt tcctccgaca aggacctgat gcagctggtg gaagacggca aggtccggct
     3601 tctcgacacc atgaagaaca agaagatcgg ttcggccgag gttgtggaac gcttcggtgt
     3661 gccgccgtcg aaagtggtgg aggtgcaggc gctggcgggc gattcctccg ataatgtgcc
     3721 gggcgtgccg ggcatcggag tgaagaccgc cgccgagctg atcaacacat acggcgatct
     3781 cgaaaccctg ctggcgcgtg ctggcgagat caaacagccc aagcgccgcc agaccttgct
     3841 ggaaaatgcc gataaggcgc ttctgtcgca aagactggtg ctgctcgacg atcatgcgcc
     3901 gctgcgccaa gagcccggcc tgttcgccgt gcgcgaaccc aatcccgcca aactgctggc
     3961 tttcctgcag gcaatggaat tcgccagcct cgccaagcgc gcgaccgccc attacggcat
     4021 cgaagacacg gatgcgcttc ctctccccaa ggaagatgcc ggtgaggctg gacagcagaa
     4081 ggcgacccct cgaccggctt cgccgaaggc tgcgccggac gacccc
//
LOCUS       DASWPU010000560         5894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_121608,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000560 DASWPU010000000
VERSION     DASWPU010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121608"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..360
                     /gene="rpsU"
                     /locus_tag="VGC27_11775"
     CDS             148..360
                     /gene="rpsU"
                     /locus_tag="VGC27_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY0283290.1"
                     /translation="MQIIVRDNNIDQALKALKKKMQREGIFREMKLRGHYEKPSEKRA
                     RERAEAIRRYRKLQRKRAQREGLLPK"
     gene            complement(435..1367)
                     /gene="dmeF"
                     /locus_tag="VGC27_11780"
     CDS             complement(435..1367)
                     /gene="dmeF"
                     /locus_tag="VGC27_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEY0283291.1"
                     /translation="MTSKTHGHADWTHGHIFLGQAHKRAEARTKLVTILTALFMVVEI
                     AAGLVFGSMALLADGVHMATHVGALGLAAGAYWLARRHAVDPRFSFGSGKFGDLAAFA
                     SAIILGLLSLGVAIESVRRFIEPVSVHYAEALFVAVVGLAVNIVSAFLLRDPHEHGDH
                     HTHHHDHNLRAAYLHVVADAATSVLAIVALASGLAFGISWADPLVGIAGALVIAIWAY
                     GLVRDSALVLLDAEDDPDLARDIKAVVEREAGAQVLDFHLWRLGPGHRGLIVSLVGGG
                     AADSEHIKRLLVERYPSLSHVTVEIAVCADCAVR"
     gene            complement(1675..1797)
                     /locus_tag="VGC27_11785"
                     /pseudo
     CDS             complement(1675..1797)
                     /locus_tag="VGC27_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272375.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
     gene            complement(1853..2635)
                     /gene="fliP"
                     /locus_tag="VGC27_11790"
     CDS             complement(1853..2635)
                     /gene="fliP"
                     /locus_tag="VGC27_11790"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY0283292.1"
                     /translation="MTKRLLILGALLVALAMPGAAHAASQAAAAGAATAGGLSIDFSG
                     GGLFTDRMMQIIALITILSVAPSILIMMTSFVRIVVVLSLLRTALGLQQSPPNAVIVS
                     LALFLTLFVMTPTLKEAYTQGIEPLVAGQITTAQAFDKAALPMKKFMLGHVRGADLKL
                     FMDMSGNKAPSPDAVALTTVAPAFMLSELKRAFEIGFLIFVPFLIIDMAVASILMSMG
                     MMMLPPVIISLPFKLIFFVLVDGWRLVSGSLVESYLHGPPPG"
     gene            complement(2632..2913)
                     /locus_tag="VGC27_11795"
     CDS             complement(2632..2913)
                     /locus_tag="VGC27_11795"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY0283293.1"
                     /translation="MDLLDIARYVGALLLVLGLIGVAGLAARRFGLPGLARPASARRL
                     KVVESLMVSPRQRLVLLRRDDVEHLVMLCPDGATVVEASITPPPAEPPA"
     gene            3232..3651
                     /gene="flgB"
                     /locus_tag="VGC27_11800"
     CDS             3232..3651
                     /gene="flgB"
                     /locus_tag="VGC27_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEY0283294.1"
                     /translation="MEVSNIPLLSMLKDRMAWLSQRQNILSQNVANADTPGYAARDLK
                     PADFERALRNATSPNRFSGALVINNPRHIAGMLPNAGVFADVETADAEASPGGNSVSL
                     EQEMIKVSDTQMQFRAATNLYAKAISMMKTAIGRPGT"
     gene            3664..4074
                     /gene="flgC"
                     /locus_tag="VGC27_11805"
     CDS             3664..4074
                     /gene="flgC"
                     /locus_tag="VGC27_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY0283295.1"
                     /translation="MDFSTSMAIAAAGMRTQSARMKVIAENIANANSTSPVVGGDPYR
                     RQIPTVKSEFDRTLNATVVGAGKPLQDQSPFRLQYDPGNPAADKQGYVKLPNVNSLVE
                     IMDMREAQRSYEADLTVMDATKTMLARTVDLLNK"
     gene            4161..4478
                     /locus_tag="VGC27_11810"
     CDS             4161..4478
                     /locus_tag="VGC27_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY0283296.1"
                     /translation="MAVLPAAAAAAYQAVSQMSTGAMPASGTVSAGGTGSFSAFLTDA
                     VKDSIDTIKHGEVMATKQVAGQANLVDVVNAVNAAELTLDTVVAVRDKVVAAYQSIMN
                     MPI"
     gene            4592..4855
                     /gene="fliQ"
                     /locus_tag="VGC27_11815"
     CDS             4592..4855
                     /gene="fliQ"
                     /locus_tag="VGC27_11815"
                     /inference="COORDINATES: protein motif:HMM:NF004671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEY0283297.1"
                     /translation="MTSADAIDFARTSIYVLLEIITPVMLTALVVGLVIGLLQALTQI
                     QEMTLVFVPKIIAIFLVLLIALPFAGQAMGGLMSDIAQRIAAY"
     gene            4863..5630
                     /gene="fliR"
                     /locus_tag="VGC27_11820"
     CDS             4863..5630
                     /gene="fliR"
                     /locus_tag="VGC27_11820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEY0283298.1"
                     /translation="MTITLPGLSGTVLVYFLVFARTGAMVMLLPAIGEAGVPARVRLI
                     VALAISLALAPTVAGSYPQQAPATVLALALLLAQEVTAGILIGAMARIIVSALSVAGF
                     LIATQTGLAYAQTLDPTTHGEQGAVVGNFFSLLGVVLIFATNLHHLAIGAIAGSYHLI
                     PPGTGLPTGDMAQLTIGLVSGSFALGFQLAAPFLVFGFAVNAAFGVLARMMPQLQVFF
                     VAMPVNILVGFILLTLLLGSMMTLYLDFYTAQMGAFL"
BASE COUNT         1176 a         1902 c         1810 g         1006 t
ORIGIN      
        1 ctaagggtca aaggggcttg agggccgatt ttcgcccttt tttggccctg gggcggcgcg
       61 gccccggtgg acaggccatg acccgtctgt tatctaccgc ggcctttaaa atcgccgctt
      121 tttggaaaat ggcaggagaa agccgctttg cagatcatcg ttcgcgacaa caatatcgac
      181 caggcgctga aagcgctgaa aaagaagatg caacgcgaag gcatcttccg cgagatgaag
      241 ctgagaggcc attacgaaaa gccaagcgaa aagcgggcac gcgagcgggc cgaggcgatc
      301 cgccgctacc gcaagctcca gcgcaagcgc gcccagcgcg agggtctgct gccaaaatag
      361 gcacgccctg tttttgagct tacttccaga ggccgtcctt cagggcggcc ttttcgtttg
      421 cgcctttgac ccgatcagcg cacggcgcag tcggcacaga cggcgatttc caccgtgacg
      481 tgactgaggc tgggatagcg ctcgaccaga agccgcttga tgtgttcgct gtcggccgcg
      541 ccgccgccga ccagcgagac gatcagaccg cgatgtcccg gccccaaccg ccacagatgg
      601 aagtccaaaa cctgggctcc cgcctcgcgt tccaccacgg ccttgatgtc gcgcgccaga
      661 tcgggatcgt cctcggcgtc gagcagcacc agggcgctgt cgcgcaccag cccgtaagcc
      721 cagatggcga tcaccagcgc gccggcaatg ccgaccagcg gatcggccca ggaaatgccg
      781 aaggcaagac ccgacgcgag cgcaacaatc gccagcacgc tggtcgccgc gtcggccact
      841 acatgcagat aggcggcgcg cagattgtga tcgtggtgat gggtgtgatg gtcgccatgt
      901 tcgtgcggat cgcgcaacag aaaagcgctg acgatattca ccgccagccc gacgaccgcg
      961 acgaacagcg cttcggcgta atgcacgctc accggctcga tgaagcggcg cacggattcg
     1021 atcgccacac caagcgaaag caagcccagg atgatggcgc tggcgaaggc ggcaagatcg
     1081 ccgaacttgc cggagccgaa ggaaaagcgc ggatcgacgg catggcgccg cgccagccaa
     1141 taggcccccg ccgccagccc caacgccccg acatgggtgg ccatgtgtac gccgtcggcc
     1201 aacagcgcca tcgaaccgaa cacaagaccc gccgcgattt cgacgaccat gaacaaggcc
     1261 gtcagaatgg tcaccaattt ggtgcgcgct tccgcccgtt tatgggcttg gccaaggaat
     1321 atgtgaccgt gagtccagtc ggcgtggccg tgtgttttgc tcgtcatgca gggcagacta
     1381 aggaccgaag ccaaaaaaga aaacgggccg cgggctctgg gttcccgcgg ccctgctcag
     1441 ccgccaaatc gatggggagt atgacggctc agttccgccg cgccgacaac cggcgcaaaa
     1501 ggcaaagttc gtgtcgcccc atcctgaata atgacagtag aatttagtat tcacgatttt
     1561 aggcgagttt tgccgttttg aaaagaagat tattttgcgc cgctgcggga tgaaagaacc
     1621 tcgtcccaga aaaaatcgct ttttcgtagg gacttaatta cggcggccca gcgggacaac
     1681 gccggcctgc gcggcttccg cgttgaactc ggtgaggccg atcgccgcgg cgaccgccgg
     1741 ctcgaagaaa ttgccggcac cggtcaaggc ggagggtgcc gcgacgcaat gcgcctcgcg
     1801 gaacgcctgc atcttctcgg cggagaactt ccggcgtctc ttgaaagggc tgtcagcccg
     1861 gcggggggcc gtgtaggtag ctttccacca gtgagccgga caccagccgc cagccatcca
     1921 ccagcacgaa gaagatcagc ttgaacggca gcgagatgat caccggaggc agcatcatca
     1981 tgcccatgct catcaggatc gaggccaccg ccatgtcgat gatcaggaac ggcacgaaga
     2041 tcagaaagcc gatctcgaag gcgcgcttca attcggacag catgaaggcc ggggcgacgg
     2101 tggtgagcgc aaccgcatcg ggcgaaggcg ccttgttgcc ggacatgtcc atgaacaatt
     2161 tcagatcggc tccgcggaca tggccgagca tgaatttctt catcggcagc gccgccttgt
     2221 cgaaagcctg tgcggtggtg atctgtccgg cgaccagcgg ttcgatgccc tgcgtgtaag
     2281 cttccttcag cgtcggcgtc atcacgaaca gggtgaggaa cagcgccagg ctgacgatca
     2341 cggcgttggg cgggctttgc tgcaggccga gcgcggtgcg cagcagcgac aagaccacga
     2401 cgatgcgcac gaagctcgtc atcatgatca ggatcgacgg cgccacgctg agaatcgtga
     2461 tcagcgcgat gatctgcatc atgcggtcgg tgaagaggcc gccgccggaa aaatcgatcg
     2521 acaatccgcc cgccgtcgcc gcgcccgcgg ccgccgcctg gctcgcggcg tgggccgcgc
     2581 cgggcatcgc cagcgcgacg aggagcgcgc ccagaataag gagacgtttg gtcatgcggg
     2641 cggctctgcg ggcggcggcg tgatcgaggc ttcgaccacc gtggccccgt cgggacagag
     2701 catcaccaga tgctcgacat catcccggcg cagcagaacc aggcgctggc gcggcgagac
     2761 catcagactc tcgacgactt tgagccggcg cgcggacgcc ggtctggcga ggccgggcaa
     2821 accgaagcgg cgcgcggcca gtccggcgac gccgatcagc cccagtacca gcagcagggc
     2881 gcccacataa cgtgcgatat cgagaaggtc catgttcgtc ttgcagcctc acccttcgac
     2941 aagctcaagg taaggaaggg cgtgtccccc gaccccccat gctgagcctg tccgccttcg
     3001 ctaatgctac ggcggacttt cctgtcctca ctcgcccgcc gcagcccttg cgcaagcggg
     3061 tcgaagcatg agggcatgcc ctatataaaa aagccgtcaa gcttaaccgc gggttaaacg
     3121 ccggaagaat ttgcccggca ggtttttccg ttttcgttaa gcgcggctta atcgttgtcg
     3181 aacaacatgg agcgtcttaa ggctaatttt ccgtatacgg agttgggagc gatggaagta
     3241 tcgaacattc cgctgctttc gatgttgaag gaccgcatgg catggctcag ccagcgccag
     3301 aacatcctgt cgcagaacgt cgccaacgcc gacacgcccg gctacgcggc gcgcgacctc
     3361 aagccggccg atttcgagcg ggcgctgagg aacgcgacct cgccgaaccg tttttcgggt
     3421 gcgctggtga tcaacaatcc gcgccacatc gcgggcatgc tgccgaatgc cggagtgttc
     3481 gccgacgtcg agacggccga tgctgaagcc tctccggggg gcaactccgt ttccctggag
     3541 caggagatga tcaaagtttc cgacacccag atgcaattcc gggccgccac caatctctac
     3601 gccaaggcga tctcgatgat gaagaccgcc atcggccggc cgggcacgta acaggagcga
     3661 agcatggatt tttcgacatc gatggcgatc gccgcagcgg gaatgcgcac acagtccgcc
     3721 cgcatgaagg tcattgccga gaacatcgcc aatgccaact cgacctcgcc cgtcgtcggc
     3781 ggcgatccct accggcgcca gattccgacc gtgaagagcg agttcgaccg cacgctgaac
     3841 gccaccgtcg tgggcgcggg aaaacccctt caggaccaga gcccgttccg cctgcaatac
     3901 gatccgggca accccgccgc cgacaagcag ggctacgtca aactgcccaa cgtcaacagc
     3961 ctcgtcgaaa tcatggacat gcgcgaagcg caacggtcct acgaagccga cctcaccgtg
     4021 atggacgcca ccaaaacgat gctggcgcgc acggtggacc tgctcaacaa atgacgcgga
     4081 gcgtcatccc cgcccccggg cccggccgat ggccggccca agggtaaatt ccagcgggga
     4141 tcctgtaaga ggagaatccg atggccgtct tgccggcagc agccgcagcc gcctaccagg
     4201 cggtttccca gatgagcacg ggcgcgatgc cagcatcggg caccgtttcc gccggaggaa
     4261 cgggaagctt ctccgccttc ctcaccgacg cggtgaagga cagcatcgac accatcaaac
     4321 acggcgaggt gatggcgacc aaacaggtcg ccggccaggc caacctcgtc gacgtggtca
     4381 acgccgtgaa tgccgccgag ctcacgctcg atacggtggt cgcggtgcgc gacaaggtcg
     4441 tagccgccta tcaatccatc atgaacatgc cgatctgaga aacattcctt caccctggtc
     4501 gatagcggca gcgtaccgac cagggtgagg gaccagcaag aaccctcatg ccgagcttgt
     4561 cgaagcatga ggccggaaca cgggaaaaac catgacctcc gccgacgcca tcgacttcgc
     4621 ccgcacttcg atctacgtgc tgctggagat catcactccg gtgatgctga ccgcgctggt
     4681 ggtcggactg gtcatcggcc tgctccaagc gctgacccag atccaggaaa tgacgctcgt
     4741 gttcgtgccg aagatcatcg cgatcttctt ggtgctgttg atcgcgctgc cttttgcggg
     4801 ccaagcgatg ggcgggttga tgagcgatat cgcccagcgc atcgcggctt attagcaccc
     4861 agatgacgat tacccttccc ggcctttccg gcacggtcct ggtctatttt cttgtcttcg
     4921 cgcgcaccgg cgcgatggtg atgctgctgc cggcgatcgg cgaagccggc gtcccggccc
     4981 gcgtgcgcct gatcgtggcc ctggcgattt cgctggcgct cgctcccacc gtggccggct
     5041 cctatcccca gcaagcgccg gccacggttc tggcgctcgc tcttctgctc gcccaggaag
     5101 tcaccgcggg catcctgatc ggcgcgatgg cgcgcatcat cgtcagcgcg ctctccgtcg
     5161 cgggcttcct gatcgccact cagaccgggc ttgcctacgc ccagactctc gatccgacga
     5221 ctcacggcga gcagggcgcc gtcgtcggca actttttttc gctgctcggc gtggtgctga
     5281 tcttcgccac caacctgcat catctggcga tcggcgcgat cgccggctcc tatcatctga
     5341 taccgccggg aacgggcctg cccaccggcg acatggccca gctgacgatc ggactggtga
     5401 gcggctcctt cgcgctgggc ttccagctgg cggcgccgtt cctggtgttc ggtttcgcgg
     5461 tgaacgcggc attcggcgtc ctggcgcgca tgatgccgca gcttcaggtc ttcttcgtcg
     5521 ccatgccggt caacatcctg gtcggcttca ttctgctgac gcttctgctc ggctcgatga
     5581 tgacgctgta cctcgacttc tacacggccc agatgggggc gttcctatga gcgcttccag
     5641 gaaaagtggc ccgcgcgcgt tgcgcgctat cttatttctt ggccgcaacg cggcccgcgc
     5701 gcgttgcgcg ctattgaatt tcttcggcgc aaagcgccgg ggccggtttt ccgtccggaa
     5761 gcgcgacaag agatagtcca tggccgacga tcgcgacccg tcccaacaaa ccgaagagcc
     5821 gaccccaaag cggctcgacg acgcgcgcaa acatggcgac gtcatcaaga gtcccgaact
     5881 gcagacgctg atcc
//
LOCUS       DASWPU010000561         4012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_17446,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000561 DASWPU010000000
VERSION     DASWPU010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17446"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            695..1540
                     /locus_tag="VGC27_11825"
     CDS             695..1540
                     /locus_tag="VGC27_11825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY0283299.1"
                     /translation="MFNLGRDRFKRGAKVGSLAVLALAIVLASLSSSPALAKRSKPRA
                     TPAGATDPAKDAALIIDGGTGKILYARNATAERHPASLTKMMTLYLLFDALKKGQMTL
                     ATPLPFSSHAASQHPTKLYVAAGESIPVDAAIRAIVVRSANDVAVAIGEALGGTEAHF
                     AEMMTAKARALGMRNTFFHNASGLPNPMQITTATDMATLARHLAYDHPEYFHYFSITD
                     FSFRGVHYVGHDKLLGRYKGTDGIKMAIPGPPASIWCRPSFATVRTSSASSWADARRR
                     AATRK"
     gene            1501..2187
                     /locus_tag="VGC27_11830"
     CDS             1501..2187
                     /locus_tag="VGC27_11830"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY0283300.1"
                     /translation="MGGRTAPRRDKEMMRLLDVTFDKIGRNPTLVARVDVPWRKMAQN
                     TVSYVPGSMAQTRLAALPSAKGKLSDADDEDSAETRSYEEGDTSDIVTESAPVPVPAP
                     ARRPVVVASYQPPSFKPTVPKPSLRPQVVESGIAGAALHSLNVHDWTIQIGAFTDLTQ
                     ARTQLATYAEKSMDILGQAQRIVVPFQSIDGQLLYRARFGPFLEREARQVCARLTERG
                     QTCFAAISTR"
     gene            2259..3028
                     /locus_tag="VGC27_11835"
                     /pseudo
     CDS             2259..3028
                     /locus_tag="VGC27_11835"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(3177..>4012)
                     /locus_tag="VGC27_11840"
     CDS             complement(3177..>4012)
                     /locus_tag="VGC27_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0283301.1"
                     /translation="LAGVVLTGSTEAAMTINRTLAARSGPIATLIAETGGQNAMIVDS
                     TALPEQVARDAVASAFDSAGQRCSALRVLFVQEDVADRMLDMVLGAMDQLQIGDPMDP
                     ATDVGPVIDEASRETLEQHAVRMTREAKLLRKLALGPEHKAGVFFPPHVFEIESMAQL
                     EREVFGPVLHVVRYAADRLDKVCEAINASGYGLTLGIHSRIEETAAFVRARAHVGNLY
                     VNRNQIGAVVGVQPFGGEGLSGTGPKAGGPHYLLRFALERTYTVNTAAVGGNAALLSS
                     S"
BASE COUNT          744 a         1328 c         1157 g          783 t
ORIGIN      
        1 tcttggtctt cgggcgcgtc ttggcggcaa cgccggttcc ggggcccgtc ccgtttccgc
       61 cgcctatggt gttccgtcct tcgttgtcgc gtttgtcgct catacccgta tccggctgac
      121 ggcccattat aagagggcga aaataaattt tcgcaactgt ccgtcgccgg cagagtttat
      181 ataggtgcga tctgcctcaa atacaggcgg ctgcgcggac gcctttgcgg aaaagaaaaa
      241 ggagccggaa agacttcccc gcccccttgt catatttgcc cgagtccggc cgcgcacatg
      301 ctctgcacgg cgtccaccca ggccagaacg cgactcgagt gcgtctttcg tcatcgcgac
      361 aaaaaatctc cgagacgttc gcgccctgcc ccaaccttgg cctatacgct cgaaatccct
      421 ttcacctgcc tggcgcctcg ctatcggcaa gcactgcatt cttgaagaat acctgcattt
      481 gttgcgatgc aatatcattt aatataggcg caccatcggc tccggcaaac gccatttgcc
      541 gtgcggcacc aagggccgtt gttagccatc cattaaccag ctgtaaatac cagaagatta
      601 acgtcaattc cgtttacggg cttgcaccgc ccgttctgat ttggtctcat tgtctgtaat
      661 ggtgagccga taacccgagg gtttggggct gtggatgttt aacctggggc gcgaccgctt
      721 caaacggggc gcgaaagtgg ggtcgttggc cgttctggcg ctcgcaatag tgctggcctc
      781 cttaagctcg tcccccgccc tggccaaacg ctccaagcct cgcgccacgc ctgctggtgc
      841 gaccgacccg gccaaagacg cggcgctgat catagacggg gggaccggca agatcctcta
      901 cgcgcgcaac gccacggccg aacgtcatcc ggcgtcgctg accaagatga tgaccctgta
      961 tctgctgttc gacgcgctca agaagggaca gatgacgctg gcgacgccgc tgccgttctc
     1021 ctcccatgca gcgtcgcaac atccgaccaa actctatgtc gctgccgggg aatcgatccc
     1081 ggtcgatgcc gccatcaggg ccatcgtcgt ccgttcggcc aacgatgtag ccgtagcgat
     1141 cggcgaggcg ctgggcggaa ccgaagcgca tttcgcggaa atgatgaccg ccaaggcccg
     1201 tgcgctcggc atgcgcaaca cgttcttcca caatgcgtcg ggcctgccca atcccatgca
     1261 gatcaccacg gccaccgaca tggcgaccct cgcccggcat ctggcctacg accatccgga
     1321 gtatttccat tatttttcga tcacggattt cagctttcgc ggcgttcatt atgtcggcca
     1381 tgacaagctt ttgggacgct acaagggcac cgacggcatc aagatggcta taccggggcc
     1441 tccggcttca atctggtgtc gtccgtcgtt cgcgacagtt cgcacctcat cggcgtcgtc
     1501 atgggcggac gcacggcgcc gcgccgcgac aaggaaatga tgcgtctgct cgacgtcaca
     1561 ttcgacaaga tcgggcgcaa tcccacgctg gtcgcgcgtg tcgatgtgcc atggcgcaag
     1621 atggcgcaga atacggtttc ttatgttccg ggcagcatgg cacagacgcg tctcgccgcc
     1681 ctccccagcg ccaagggcaa gctgtccgac gccgacgacg aagacagcgc cgaaacccgc
     1741 agctacgagg aaggcgatac ctccgacatc gtgaccgaat cggcaccggt accagtgccg
     1801 gcgcctgcgc ggcggccggt ggtcgtggcc tcctatcagc cgccgagttt caaacccacc
     1861 gtccccaagc cgtccttgcg tccgcaagtc gttgaaagcg gcattgccgg ggcggcgctg
     1921 cattcgctga acgtccacga ctggaccatt cagatcggtg cttttaccga tctcacgcag
     1981 gcgcggacac agcttgccac ctatgcggaa aagtcgatgg acattctggg acaagcccag
     2041 cggatcgtcg tgccattcca atcgatcgac ggccagttgc tctaccgcgc ccgcttcggc
     2101 ccgttcctgg agcgcgaagc gcgccaagtt tgcgcccgcc tgaccgagcg cggccagacc
     2161 tgcttcgccg cgatctcgac gcgctaggag acatacccat aaaggggatt cccatttttg
     2221 gcgaacagtg attcaaatct ccaacgctgg aggtttggat ggctcgcttc gatttgacgg
     2281 acttcgagtg gtcggtgata gcaccgctgc tgccgaacaa accgcgcggt gttgcccggg
     2341 tggacgaccg ccgggttttg aacgggatat tctggcgcct gcgtaccgga gcgccgtggg
     2401 cggatattcc cgaccgttat gggccgcaca cgacctgcgt gaaccgcttc aaccggtggc
     2461 gccgggcagg cgtctgggat cgcattctgg ctgcggtttc agaggcttat aagggcgatg
     2521 tccagatgat cgattcgtca tccatccggg tccatcagca tgccgccaac ggcaaaaaaa
     2581 acagacgcga tcccgttgca tgggtcgctc gcggggcggg ctgacgacca aaatccacgc
     2641 tcttgtcgat gcctgtggcc tgccgatcct tctgaagctc accgagggcc aagctcatga
     2701 tggccgcagc gcccaggaca tgctcggcac gcttggccgc ggcgacgttc tgctggggga
     2761 ccgcgcctac gattccgacg ccctgcgcca agacctcgcc gcgcgcggcg cacgagccaa
     2821 tgtccgactg atgccgaacc ggacgaacct gccgcccttc aacaagcgcc tttatcgcca
     2881 gcgcaatctg gtcgaacgct tcttcaacaa actgaagcat tttcgagcgg tcgccacccg
     2941 atacgacaag cgcgacgaca actatctcgc atccgtcaaa ctcgcctcaa tccgtatctg
     3001 gatccgcttt aatgagtcgg tctcctagag tatttcaccg tttcacggaa acgatgaaat
     3061 actctagatt cttggtttgt cgcgttttcg gacggaaaac cgcccggcgc gccttgcgcg
     3121 cctattggtt ccatagcgcg ctttcgcgcg ggcacttttc ctgaaaacgc tctaattcaa
     3181 ctcgaactca gcagcgccgc gttgccgcct accgccgccg tgttcacggt gtaggtgcgt
     3241 tcgagcgcga agcgcagcag ataatgcggc ccgccggctt tcggtccggt gcccgacagt
     3301 ccctcgccgc cgaacggctg cacgcccacc acggcgccga tctggttgcg gttgacgtag
     3361 agattgccga catgcgcccg cgcccgcacg aaggcggccg tttcctcgat gcggctgtgg
     3421 atgccgagcg tcagcccgta gccgctggcg ttgatcgcct cgcagacctt gtcgagccgg
     3481 tcggccgcat agcgcaccac gtgcagcacc ggaccgaaca cttcgcgctc caattgcgcc
     3541 atcgactcga tctcgaaaac atgcggcggg aagaacaccc ccgccttatg ttccggcccg
     3601 agcgccagct tgcgcagcaa cttcgcttcg cgcgtcatgc gcaccgcatg ctgctccagc
     3661 gtttcgcgcg acgcctcgtc gatcaccggc ccgacgtcgg tcgccgggtc catcggatcg
     3721 ccgatctgca attggtccat tgcccccagg accatgtcga gcatcctatc ggcgacgtct
     3781 tcctgcacga acagcacgcg caaggccgag caacgctgac cggcgctgtc gaaggcggaa
     3841 gcgaccgcgt cgcgcgccac ctgttccggc aaagccgttg aatccacgat catcgcgttc
     3901 tggccgccgg tctcggcgat caaggtcgcg atggggccgc tgcgggccgc cagcgtgcgg
     3961 ttaatcgtca tcgccgcttc ggtcgagccg gtcagtacca cgccggccaa gc
//
LOCUS       DASWPU010000562        11297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_66762,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000562 DASWPU010000000
VERSION     DASWPU010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66762"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VGC27_11845"
     CDS             complement(<1..883)
                     /locus_tag="VGC27_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283302.1"
                     /translation="MTRERLFLFDTTLRDGAQTQGVDFSVEDKRQIALALDALGIDYV
                     EGGWPGANPTDTAFFAERPPLKRARFTAFGMTKRAGRSLSNDPGFAAVLEADTDAVCL
                     VGKSWDFHVDVALEIPRSENLEGIAESVAAVKAKGREPLFDAEHFFDGYKANPDYALQ
                     CLRAAHDAGARWLVLCDTNGGSMPEDVSRIVSEVKAKLPDAALGIHAHNDTEQAVAIS
                     LAAVRAGARQVHGTLNGLGERCGNANLCTLLPTLMLKEPFASEFETGVSKEQLARLTH
                     VSRLLDEILNRAANRHAP"
     gene            complement(1029..2444)
                     /locus_tag="VGC27_11850"
     CDS             complement(1029..2444)
                     /locus_tag="VGC27_11850"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY0283303.1"
                     /translation="MTLRLYDTLTKQKQTLEDLPGFDRNNVRMYVCGPTVYDFAHIGN
                     ARPVIVFDVLFRLLKHLYEKDGGKVTYVRNVTDIDDKINARAAETAAAKGISVEAARA
                     EITTATAKQFDEDVAALGCLPPTETPRATQYVPQMIAMIETLVAAGHAYVADPPTPEA
                     SADKSHGPAGALAKAGGHVLFDVSSKPDYGKLAKRSLDEMLAGARVEVAPYKKNPMDF
                     VLWKPSDASTPGWESPWGRGRPGWHIECSTMGGALLGDTFDIHGGGIDLMFPHHENEI
                     AQSEGAHHGRPLARVWMHNGFLQVEGEKMSKSLGNFITIRDLLKDWPGEVLRFNMLRT
                     HYRQPIDWTLNGLKESHQTLERWYPHAATRQGSPLPGEFVDALLDDLNTPKAIAALHE
                     HAPSADALKLLGFSADPSKIARSVVSALPEGEIAAAIEARAAARKAKNFKESDRIRDE
                     LLAKGIVLKDGPTGTTWEVKR"
     gene            complement(2750..4090)
                     /gene="gltX"
                     /locus_tag="VGC27_11855"
     CDS             complement(2750..4090)
                     /gene="gltX"
                     /locus_tag="VGC27_11855"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY0283304.1"
                     /translation="MTDTTVRFAPSPTGLLHVGNARTALLNFLFARKDGGKFLLRIDD
                     TDAERSKPEYEAAILEDLNWLGIAHDLFARQSERAEAHRAAAEKLKAAGRLYPCYETA
                     LELDRRRQRQIAAGHPPVYDRAALKLSDEERSKLKAQGRKAHWRFKLSQSKVKWRDLI
                     RGEVEIDTAHLSDPVLIREDGRFLYTLPSVVDDVDFAISHIIRGEDHVTNTAVQIEIF
                     EALGAAVPAFAHHPLLIGAGGEALSKRLGSLSLRLLREDGIEPITADSYLAKTGTSDA
                     ITPHAAMDELVAGFALEKIGRAPAHFDVAELSNLNAKLLHALPFEAVAARLAALGIDG
                     GAPFWEAVKPNLARFADASDLWTLVQGPIAPVIEDAPVIAEAAALLPPEPWDEATWNA
                     WTGAVAAATGAKGRALYHPLRLALTGRDHGPELKKLLPLIGRARAYARLQGETA"
     gene            complement(4197..4538)
                     /locus_tag="VGC27_11860"
     CDS             complement(4197..4538)
                     /locus_tag="VGC27_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283305.1"
                     /translation="MAEADFLKHGGKLITTKDVDLHNLDQAIPSFFCDRVACFGKVAF
                     EFEVGEDGAPRNVRVTENTWKDRPTEHAAVLEERLLASRYAPPKLRRKLVCIETDWQL
                     VFEAAEVKRQP"
     gene            complement(4709..6361)
                     /locus_tag="VGC27_11865"
     CDS             complement(4709..6361)
                     /locus_tag="VGC27_11865"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY0283306.1"
                     /translation="MTDRLRIALAQLNPCMGDIAGNLAKARAARAEAGDADVLLFSEL
                     FITGYPPEDLVLKRALQDEAREAVQAFAKDTVGGPAVLIGMPWREGGKLYNAVALLDG
                     GRIAARTFKHDLPNYGVFDEKRVFAPGPLTGPLEVRGVRIGVPICEDIWTGEVTECLA
                     ETGAEILLVPNGSPYETGKEDVRLNLVTARVTETRRPLAYLNQVGGQDELVFDGASLV
                     VNADCSVAWALPAWREKIALTEWRRQGEGWVCLPGERAPEEERSQSVYHAMMLGLRDY
                     VNKNNFSGVVLGLSGGIDSALSAAVAVDALGRERVRCVMMPSRYTAQDSLDDAEECAQ
                     LLNVSCETIPIERAVDSFADTLRPLFAGRGADTTEENIQSRIRGLILMALSNKFGPMV
                     LTTGNKSEMSVGYATLYGDMCGGYNVLKDIYKMEVFRLARWRNQMLPSGAMGPPGRVI
                     PERIIAKPPSAELRANQTDQDSLPPYEILDGILECLVEKEMAFEDVVAKGFDPATVRR
                     VEHLLYISEYKRRQAPPGVKIGPRNFGRDRRYPITNAFRDAR"
     gene            complement(6415..7389)
                     /locus_tag="VGC27_11870"
     CDS             complement(6415..7389)
                     /locus_tag="VGC27_11870"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0283307.1"
                     /translation="MSPFRFARQNAPLKVAVVGAGAFGRHHATKYRGLEGVELLAIAD
                     PSAEVRKSATATYDVPAVADWRDLLGKVDLVSVASPAVTHAEIVRAFLNAGAHVLVEK
                     PIATSIEEADALITLAERMGLILTVGHQERFVFAHTGLLDYDDTPLEVSCWRQGPWSG
                     RGADVSVVLDLMIHDLDLVHQLIPGKVRNVRARGRIEKSSFADEVSAAVLFDNGAVAR
                     FETSRIAETRRRGMRAVYADGIVEIDFLTRKVTNTTPRALKALEVGDPLAESVAAFVA
                     AARSGQPPLVRPRQARRALETALLIDEASERSGALRSRQELVAYAAAN"
     gene            7536..8552
                     /locus_tag="VGC27_11875"
     CDS             7536..8552
                     /locus_tag="VGC27_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY0283308.1"
                     /translation="MGSEKNRNVRLIRRPLGLPAPADFQLVESQIPSPNPGQVLVRNL
                     FMSVDPYMRGRMADRKSYFPPFQLGEVLQGRAVGKVTAGEAGDLRPGDYVSSMNGWRE
                     WFVSNGEGLKKIDPASAPLEAYLSVLGATGMTAYIGLTRIDKVKDGDRVFVSDAAGAV
                     GMTACQIARNRGAHVIGSAGSDEKCAFLRDELKIGAINYRKTSDLAAALADAFPAGID
                     LYFDNVGGGHLAAALDNMRVGGRIVVCGMIGQYNTVSPPPAPVNLLNIGVRSLTMRGF
                     IFTDHLDLTEPFEREMRAWIAEGKMIWRQTVITGIENAPAAFIGLFKGENFGKMLVKL
                     GEDE"
     gene            complement(8621..8914)
                     /locus_tag="VGC27_11880"
     CDS             complement(8621..8914)
                     /locus_tag="VGC27_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY0283309.1"
                     /translation="MQKHYWTYIMASARNGTLYIGVTNDLSRRVWEHREGLVPGFTKK
                     YGVKMLVYFEIFEDVNAAIHQEKLWKKWKRRWKLELIEKRNPEWRVLAETLNA"
     gene            complement(9075..9824)
                     /locus_tag="VGC27_11885"
     CDS             complement(9075..9824)
                     /locus_tag="VGC27_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283310.1"
                     /translation="MGVARLLAKGWVLVCLYAGAHALNFVVQGSADPLGALPSLIGCV
                     VLFSAMGLLFIAGYGATAGQSGVPLVRRFMPLRYIPGFNDIVFLVFVCLSFVNQITVA
                     PANLTGTVVDGFEKAMYFAVPGQRALADTLGACALDGGRIFAAAIAWLLAVVFLCSSF
                     SHLKHDARILRLERAMRPDALGPRAVAIMLGIAAVVGFQLLFVGSAAAFLPCSAYADL
                     SGALLIGLAPLLLAYIIPAALTAALAAGRER"
     gene            complement(9850..11229)
                     /locus_tag="VGC27_11890"
     CDS             complement(9850..11229)
                     /locus_tag="VGC27_11890"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEY0283311.1"
                     /translation="MAQSWSPQSWRSKPICQVPVYPDAAALAAVEAKLRTHPPLVFAG
                     EARKLKASLAQVAGGKAFLLHGGDCAESFAEFHANNIRDTFCVFLSMAMVLTFAVNLP
                     VVKVGRIAGQFAKPRSDDIETRDGAVLPSYRGDIINGEGFTAEDRKPDPERMIRAYFQ
                     AAATANLVRAFATGGYADLSRARYWYLDNHHHHDLLQRYLAVVEKIADGIEFLRTFAL
                     ADTVKQQTNRVDFYFSHEALLLPYEEALTRQDSITKENYATSGHMLWIGDRTRQPDGA
                     HVEYCRGIKNPLGLKCGPSMREDDLLKLVALLNPDNEPGRLTLITRFGADKVAAHLPR
                     LIRAVKREGFKLVWSCDPMHGNTVKSSTGYKTRPFDRVLQEVKTFFDVHRAEGTYAGG
                     VHLEMTGQNVTECMGGAQDITDVDLHDRYNTRCDPRLNASQALELAFLLAESLREEAL
                     KAAGPPAPA"
BASE COUNT         2003 a         3656 c         3628 g         2010 t
ORIGIN      
        1 aaggcgcgtg acggttggcg gcgcggttga ggatttcgtc cagcagccgc gaaacatgcg
       61 taagccgcgc caattgttcc ttggagacgc cggtttcgaa ctcagaggcg aacggctcct
      121 tcagcatcag cgtcggcagc agggtgcaga gattggcgtt gccgcagcgc tcgcccagcc
      181 cgttgagcgt tccatggacc tggcgcgccc cggcgcgtac cgccgcgagg ctgatggcga
      241 cggcctgttc ggtatcgtta tgggcgtgga tgccgagcgc cgcgtccggc agcttcgcct
      301 tcacctcgga aacgatgcgc gaaacgtcct ccggcatgga gccgccgttg gtgtcgcaca
      361 gcaccagcca gcgcgcgccc gcgtcatgcg cggcacgcaa gcactgaagc gcgtaatcgg
      421 gattggcctt gtaaccgtcg aagaaatgct cggcgtcgaa cagcggttcg cggcccttgg
      481 ccttcaccgc cgcgacggat tccgcgatgc cttccagatt ctcggaacgc gggatctcca
      541 gcgccacatc gacgtggaag tcccagctct tgccgaccag acagaccgca tccgtatcgg
      601 cctccagcac cgccgcgaaa ccgggatcgt tggagaggct gcgtccggcg cgcttggtca
      661 tgccgaaagc ggtgaagcgc gcgcgcttga gcggcgggcg ttcggcgaag aaggcggtgt
      721 cggtcgggtt ggcgccgggc cagccgcctt cgacatagtc gatgcccagc gcatcgagcg
      781 ccagcgcgat ctggcgcttg tcttccaccg agaaatcgac gccctgggtc tgcgccccgt
      841 cgcgaagggt ggtgtcgaag aggaagaggc gttcgcgggt catcgcaaca cctcttttgt
      901 ttcgtcatgg ccgggcttga cccggccatc cagccggcga acgtccgcga gccgaaagac
      961 tctggatggc gtccttacgc tgtcgctcag gaccaagggc ggccatgacg aataggaggg
     1021 ttgagcattc atcgcttcac ctcccaggtc gtgccggttg ggccgtcttt cagcacgatc
     1081 cccttcgcca gtagttcgtc gcggatgcgg tcggattcct tgaagttctt cgccttgcgc
     1141 gcggcggcgc gcgcttcgat agcggccgca atttctcctt cgggaagcgc cgagacaacg
     1201 gaacgggcga tcttcgacgg atcggccgag aaccccaaca gcttcaacgc atccgccgac
     1261 ggcgcgtgtt catggagggc ggcgattgct ttcggcgtgt tcaggtcgtc aagcagcgcg
     1321 tcgacgaatt cccccggcaa cggcgacccc tgacgcgtcg cggcgtgcgg ataccagcgc
     1381 tccagggttt gatgcgattc cttcaaccca ttcagagtcc agtcgatggg ttggcgataa
     1441 tgcgtgcgca gcatgttgaa gcgcagcact tcgcccggcc agtccttcag caggtcgcga
     1501 atggtgatga agttgccgag cgacttcgac atcttctcgc cctcgacctg gagaaagccg
     1561 ttgtgcatcc agacgcgcgc cagcgggcgg ccgtgatggg cgccttcgct ctgggcgatt
     1621 tcgttttcgt gatgggggaa catcaggtcg atgccgccgc cgtggatatc gaaagtgtcg
     1681 ccgagcagcg ccccgcccat cgtcgagcat tcgatgtgcc agccgggacg gccgcggccc
     1741 cagggcgact cccagccggg cgtggaggcg tccgaaggct tccacagcac gaagtccatc
     1801 gggtttttct tgtaaggcgc gacttccacc cgcgcgccgg ccagcatttc gtccagcgag
     1861 cgcttcgcca gcttgccgta gtcgggcttg gacgagacgt cgaacagcac atggcctccc
     1921 gccttcgcca aggctccggc gggtccgtga gacttgtccg ccgaagcctc aggcgtaggc
     1981 ggatcggcca cgtaggcatg gcccgccgcg accagcgttt cgatcatcgc gatcatctgc
     2041 ggcacatatt gggtggcgcg cggcgtttcg gtcggcggca ggcagcccag cgccgccacg
     2101 tcttcgtcga actgttttgc cgtggccgtc gtgatttccg cccgcgccgc ttcgacggag
     2161 atgcccttcg cggcagccgt ttccgccgcc cgcgcgttga tcttgtcgtc gatgtcggtg
     2221 acgttgcgga cataggtgac tttgccgccg tccttctcat agagatgctt gagcagccgg
     2281 aacagcacgt cgaacacgat caccggccgc gcgttgccga tatgggcgaa atcgtagacc
     2341 gtcggcccgc agacatacat gcggacattg ttgcggtcga agcccggcag gtcttcgagc
     2401 gtctgctttt gcttggtcag cgtgtcgtag aggcgaagcg tcatgaattg tcccttacca
     2461 caatttgctc gcggagacgc ggaggcacgg aggcgggccg gtttcttctc cgcgttctcc
     2521 gcgactccgc gtgatctgat gttgccatag gggtgtttga atcaacgatg ttgacccgct
     2581 gtacgggccc gaattgttct gaagatgtag gcgagcaaaa gggtgtatcg cggcggtcag
     2641 ccgccttcgc tttcagcttc ggcgggccaa gtgtgcctca cttgccgcgg cgtagccaaa
     2701 ggcgaagccg ggccgcaaat tcgggtcgac atcatctggc ggaactggtt cacgccgttt
     2761 ccccttgaag ccgcgcgtag gctcgcgcgc gcccgatcag gggtagcagc tttttcagct
     2821 ccggtccatg gtcgcggccg gtcagcgcca ggcgcagcgg atggtaaagc gcccggccct
     2881 tggcgccggt agcggcggcg accgcccccg tccaggcgtt ccaagtggct tcgtcccaag
     2941 gttccggcgg caacagcgcg gcggcctcgg cgatgaccgg cgcatcctcg atcaccggcg
     3001 caattgggcc ttgcaccagc gtccaaaggt cgctcgcgtc ggcaaagcgc gccaggttcg
     3061 gcttcaccgc ctcccagaac ggcgcgccgc cgtcgatgcc aagcgccgcc agccgcgccg
     3121 ccaccgcctc gaacggcagg gcatgcagca gcttggcgtt gagattggac agttccgcca
     3181 cgtcgaaatg ggcgggggcg cggccgatct tctccagcgc gaagcctgcg accagctcat
     3241 ccatcgcggc gtgcggcgtg atggcgtcgg aggtaccggt cttcgccaaa taggagtccg
     3301 ctgtgatcgg ctcgatgccg tcctcgcgca gcaggcgcag cgacagcgac cccagccgct
     3361 tcgacaaggc ctcgccgccc gcgccgatca gcagcggatg atgggcgaag gcggggacgg
     3421 ccgcgccgag cgcttcgaaa atctcgatct gcaccgccgt gttggtgacg tgatcctcgc
     3481 cgcggatgat atggctgatg gcgaaatcga catcgtccac gaccgacggc aaagtgtaga
     3541 gaaaacgccc gtcctcgcgg atcagcaccg gatcggaaag atgcgcggtg tcgatctcca
     3601 cctcgccgcg gatcaggtcg cgccacttca ccttcgactg cgaaagcttg aagcgccaat
     3661 gcgccttgcg gccttgcgct ttcagcttgg agcgctcttc gtccgaaagt ttcagcgcgg
     3721 cgcggtcgta aaccggcgga tgacctgccg caatctggcg ctggcggcgg cggtcgagct
     3781 ccagcgcggt ctcgtaacag ggataaagcc gccccgccgc cttcagcttt tccgccgccg
     3841 cgcgatgggc ctcggcgcgc tcggactggc gcgcgaacag atcgtgggcg atgccgagcc
     3901 agttcaggtc ttcgaggatg gcggcttcgt attccggctt ggaacgttcg gcgtcggtgt
     3961 cgtcgatgcg cagcaggaac ttgccgccgt ccttgcgcgc gaacaggaaa ttcagcagcg
     4021 ccgtcctcgc attgccgaca tgcagcagcc cggtgggcga aggggcgaaa cggacggtgg
     4081 tgtcagtcac ggtgaggcct aacaaaaaat atagttgtca tccccggccg agcgacgctt
     4141 ttgcgtcgcg aggggaaggg gatccaggtg catagagaag gatgaaacgg tgaagatcat
     4201 ggttgacgct tcacctccgc agcctcgaat acaagctgcc agtccgtctc aatgcaaacc
     4261 agtttcctgc gcagcttggg cggagcatac ctcgaggcta agaggcgctc ctccaggacc
     4321 gccgcatgct ctgtggggcg gtctttccat gtgttctcgg tgacacgcac attccgcggg
     4381 gcgccatctt ctccaacctc gaattcaaac gccaccttcc cgaagcaagc gacgcgatca
     4441 cagaaaaatg aagggatggc ttggtccaga ttatggaggt cgacgtcttt ggtagtaatg
     4501 agcttgccgc cgtgcttcaa aaagtccgct tcagccatac aagcccgctg cgtggggtgc
     4561 gccgtccgtg ccacagagtt tcccgtcgtc gcaatgagca gaagcagcac gcaaatcccc
     4621 gacgttgtgc ggaccgcata acctgggtcc ccttccctcg cacggatcgc tccgcgatcc
     4681 atactcgccg gggatgacag cttatttttc atctcgcatc cctgaacgca ttggtgatcg
     4741 gatagcgccg gtcgcggccg aaattgcgcg ggccgatctt cacgccgggc ggcgcctggc
     4801 ggcgcttata ctccgatata taaagaaggt gttccacccg ccgcaccgtc gccgggtcga
     4861 agcccttggc caccacgtcc tcgaacgcca tttccttctc gaccaggcat tcgagaatgc
     4921 cgtcgaggat ctcgtagggc ggcagcgaat cctggtcggt ctggttcgcg cgcagttcgg
     4981 cgctcggcgg cttggcgatg atgcgttccg gaatgacgcg ccccggcggc cccatcgcgc
     5041 cggacggcag catctggttg cgccagcgcg ccagccggaa cacctccatc ttgtagatgt
     5101 ccttgagcac attgtagccg ccgcacatgt cgccgtagag cgtggcgtag ccgacgctca
     5161 tctcgctctt gttgccggtg gtcagaacca tcgggccgaa tttgttggag agcgccatca
     5221 ggatcaggcc gcggatgcgc gactggatgt tctcctcggt ggtgtcggcg ccgcggccgg
     5281 cgaacagcgg gcgcagcgtg tcggcgaagc tgtccaccgc gcgctcgatg ggaatggtct
     5341 cgcagctcac gttcagcagt tgcgcgcatt cctcggcgtc gtcaaggctg tcttgcgccg
     5401 tatagcgcga cggcatcatc acgcagcgca cccgctcgcg ccccagcgca tcgacggcca
     5461 ccgccgccga cagcgcggaa tcgatgccgc ccgaaagccc cagcaccacg ccggaaaaat
     5521 tgttcttgtt cacgtaatcg cgcaggccca gcatcatggc gtgatagacg gactgcgaac
     5581 gctcttcttc gggggcgcgt tctccgggca ggcagaccca gccctcgcct tggcgccgcc
     5641 attcggtgag cgcgatcttc tcccgccaag caggcagcgc ccaggccacg ctacagtccg
     5701 cattgacgac cagcgaagcg ccgtcgaaca ccagttcgtc ctggccgccc acctgattga
     5761 gataggccag cggccggcgc gtttcggtca cccgcgcggt gacgagattg agccgcacgt
     5821 cttccttgcc cgtttcgtaa ggcgaaccgt tgggcaccag cagaatctcg gcgccggtct
     5881 cggccaggca ctccgtcact tcgccggtcc agatgtcctc gcagatcggc acgccgatgc
     5941 gcacgccgcg cacctccaag gggccggtga gcggccccgg cgcgaacacc cgcttctcgt
     6001 cgaacacacc gtaattgggc aggtcgtgct tgaaggtgcg cgcggcgatg cgcccgccgt
     6061 cgagcagcgc caccgcattg tagagcttgc cgccttcgcg ccagggcatg ccgatcagga
     6121 cggcggggcc gcccaccgtg tccttggcga aggcttggac ggcctcgcgc gcctcgtctt
     6181 gcaaggcccg cttcagaacg aggtcttccg gcggatagcc ggtgatgaac agttcggaga
     6241 atagaagaac gtcggcatcg cccgcctcgg cccgcgccgc gcgcgccttg gcgagattgc
     6301 cggcgatgtc gcccatgcag ggattgagct gggccagagc aatgcgaaga cgatccgtca
     6361 tcgctgctgt ttacagcgcc gcgcggcgga taaaaaggtg cagccttggt tgcgtcagtt
     6421 cgctgccgcg taggcgacca gctcctgccg gctgcgcagc gcgccggaac gctccgacgc
     6481 ctcgtcgatc agcagcgccg tttccagggc gcggcgggcc tgccgcggac ggaccagcgg
     6541 cggctggccg ctgcgggccg ccgcgacgaa ggccgcgacc gattcggcca gcggatcgcc
     6601 cacttcgagc gctttcagcg cgcgcggggt ggtgttggtg accttgcggg tgaggaaatc
     6661 gatttccacg atgccgtcgg catagaccgc gcgcatgccg cggcggcgcg tttcggcgat
     6721 ccggctggtt tcgaaccgcg ccacggcgcc gttgtcgaac agcacggcgg ccgaaacttc
     6781 gtcggcgaag ctgctttttt cgatgcgccc gcgcgcccgc acgttgcgga ccttgcccgg
     6841 aataagctga tggacgagat cgagatcgtg gatcatcaga tcgagcacga cgctgacgtc
     6901 cgcgccgcgg ccggaccaag gtccctggcg ccagcacgac acttccagcg gggtgtcgtc
     6961 atagtcgagc aggccggtat gggcgaaaac gaaccgttcc tgatggccga cggtgagaat
     7021 caggcccatc cgctcggcca gggtgatcag cgcgtcggct tcctcgatgc tggtcgcaat
     7081 cggcttctcc accaggacat gcgccccggc gttgaggaaa gcgcgcacga tctcggcatg
     7141 ggtcacggcc ggcgaggcca cgctgaccag atccaccttg cccaagagat cgcgccagtc
     7201 ggcgaccgcg ggcacatcgt aggttgccgt cgcgctcttg cgcacctccg ccgacgggtc
     7261 ggcaatggcc agcaactcga cgccctccag gccgcgatac ttcgtggcgt gatggcggcc
     7321 gaacgctccg gcgccgacaa cggcgacctt gagcggcgca ttctgacggg caaaacggaa
     7381 gggagacaaa ggaaaatccc cggaatcaca gcgacataag cgcctttgtc ggcttccggg
     7441 gcgagtcata aaatgtttcg tcttgttaca cccaggctcg ctgctaggtt accggccttt
     7501 aggaatcggc cgcctggatg gttaaggaga tgcccatggg ctccgaaaag aaccggaacg
     7561 tccgcctgat acgccgcccg ttagggctgc ccgccccagc cgatttccag ctggtggaga
     7621 gccaaattcc ctccccaaac cctggccaag tgttggtccg gaacctgttc atgtccgtcg
     7681 acccctacat gcgcgggcgc atggcggacc gcaaaagcta cttcccgccc ttccagctcg
     7741 gcgaggtgct gcagggccgc gcggtgggca aggtgacggc cggcgaagcg ggcgacctgc
     7801 gccccggcga ttatgtgtcc agcatgaacg gctggcgcga atggttcgtc tcgaacggcg
     7861 aaggcctgaa aaagatcgat ccggccagcg ccccgctgga agcttatttg agcgtgctgg
     7921 gggcgacggg gatgaccgcc tatatcggcc tgacgcgcat cgacaaggtc aaggacggcg
     7981 accgggtatt cgtttcggac gccgccggcg cggtaggcat gaccgcctgc cagatcgccc
     8041 gcaacagagg ggcccatgtg atcggttcgg ccggctcgga cgaaaaatgc gctttccttc
     8101 gcgacgaact gaaaatcggc gccatcaact accgcaaaac cagcgatctt gccgccgctt
     8161 tggccgatgc gtttcccgcc ggcatcgacc tgtacttcga caatgtcggc ggcggccatc
     8221 ttgccgcggc gctcgacaat atgcgcgtgg gcggacgcat tgtcgtctgc ggcatgatcg
     8281 ggcagtacaa taccgtttcg ccgccgcccg cgcccgtcaa cctcctcaac atcggcgtac
     8341 gcagcctgac gatgcgcggc ttcatcttca ccgaccatct cgatctcacc gagccgttcg
     8401 agcgcgagat gcgggcctgg atcgccgaag gaaagatgat ctggcggcag acggtcatca
     8461 cgggcatcga gaatgcgccg gccgccttca tcggcctctt caaaggcgag aatttcggca
     8521 agatgctggt gaagctgggc gaagacgaat agcgtgcgac ttggaagatt cttccctcat
     8581 tgtcatgacc cgcgaaagcg gccagccaga ggacgcacgc tcaagcgttc aacgtctcgg
     8641 caaggacgcg ccattcggga ttgcgttttt cgatcaattc cagcttccag cgccgcttcc
     8701 atttcttcca gagtttttcc tgatggatgg ccgcgttcac gtcttcaaaa atctcgaaat
     8761 aaaccagcat cttcacgccg tatttcttcg tgaatcccgg aaccagaccc tcgcgatgct
     8821 cccacacacg gcgggaaaga tcgttggtca caccgatgta aagcgtgccg ttgcgtgcgc
     8881 tcgccataat gtaggtccaa tagtgctttt gcattgcggg cgaccgcttt cactttcctc
     8941 taataatgtc atggcccacg aaagttggcc atccagctga cacatcatgg attttgtcag
     9001 tttcaactgg atggcctgct tttgcaggcc atgacatccg aaggaaacgg aggattctcc
     9061 tttcctgata atttctatct ctcccgcccg gccgccagcg ccgcggtcag cgccgccgga
     9121 atgatgtagg caagcagcag cggcgccagc ccgatcagca gggcgccgga aagatcggca
     9181 taggcgctgc acggcaggaa ggcggccgcg gagcccacga acagaagctg gaagccgacc
     9241 accgcggcga tgcccagcat gatcgcgacc gccctcggcc ccagcgcgtc ggggcgcatc
     9301 gcccgttcca gccgcaggat gcgggcatcg tgcttcagat gcgagaacga cgagcacagg
     9361 aaaacgaccg ccagcagcca ggcgatggcc gcggcgaaga tccgtccgcc atcgagcgcg
     9421 caggcgccca gcgtatcggc cagcgcccgc tggcccggca ccgcgaaata catcgccttc
     9481 tcgaagccgt ccacgaccgt tcccgtaagg ttggcgggcg cgacggtaat ttggttgacg
     9541 aaactcaggc agacgaagac cagaaacacg atgtcgttga agcccggaat gtagcgcagc
     9601 ggcatgaagc gccggaccag cggcacgccg ctctgcccgg cggtcgcgcc gtaaccggcg
     9661 atgaacaaca ggcccatcgc ggaaaaaaga acgacgcaac caatcaaaga cggcagcgcg
     9721 ccgagcggat cggcgctgcc ctggaccacg aaattcaagg catgcgcgcc cgcgtacagg
     9781 cagaccagca cccaaccctt cgccaagagg cgcgctacgc ccataagcct acgccccccg
     9841 ccgcgcggtt cacgccggtg ccggcggacc ggcggctttc aacgcctctt cgcgcaggct
     9901 ttcggccagc aggaaagcaa gttcgagcgc ctggctcgca ttgagccgcg gatcgcagcg
     9961 cgtgttgtag cggtcgtgca gatcgacgtc ggtgatgtcc tgcgcgccgc ccatgcattc
    10021 ggtgacgttc tggccggtca tctccaaatg cacgccgccc gcataggttc cttcggcgcg
    10081 atgcacgtcg aagaaggtct tcacttcctg cagcacgcgg tcgaacggcc gcgtcttgta
    10141 gccggtggag gatttcacgg tgttgccgtg catcggatcg caggaccaca ccagcttgaa
    10201 cccctcgcgc ttgaccgcgc gaatgagccg cggcaggtgc gcggcaacct tgtccgcgcc
    10261 gaaacgcgtg atcagcgtca gccggcccgg ttcgttgtcg ggattgagca gcgcgacgag
    10321 cttgagaagg tcgtcctcgc gcatggaagg cccgcatttg aggccgagcg gattcttgat
    10381 gccgcggcaa tactcgacat gcgcgccgtc cggctggcgc gtgcggtcgc cgatccacag
    10441 catgtggccg ctggtggcgt agttttcctt ggtgatggaa tcctgccggg tcagcgcttc
    10501 ctcgtaaggc agcagcagcg cctcgtgact gaaatagaaa tcgacccggt tggtctgctg
    10561 cttgaccgta tcggcgagcg cgaaggtgcg caggaattcg atcccgtccg cgatcttttc
    10621 gaccaccgcg agatagcgct ggagcagatc gtgatgatga tggttgtcga ggtaccaata
    10681 gcgcgcgcgc gacaggtcgg catagccgcc ggtggcgaag gcgcgcacca gattggccgt
    10741 ggccgccgcc tggaaatagg cgcggatcat ccgttcggga tcgggcttgc gatcctcggc
    10801 ggtgaacccc tcgccgttga tgatgtcgcc gcgataggac ggcagcacgg cgccgtcgcg
    10861 cgtttcgatg tcgtcggagc gcggcttggc gaactggccg gcgatgcgcc ccaccttcac
    10921 caccggcaga ttgacggcga aggtcagcac catcgccatg ctgaggaaaa cgcagaaggt
    10981 gtcgcggata ttgttggcgt ggaactcggc gaaactctcc gcgcaatcgc cgccgtgcag
    11041 caggaaagcc ttgccgccgg ccacctgggc cagcgacgcc ttcaatttcc gcgcctcgcc
    11101 ggcgaacacc aggggcggat gggtcctgag cttggcttcc acggccgcga gcgccgccgc
    11161 gtccggatag accggaacct ggcagatggg cttggaacgc cagctttgcg gggaccagga
    11221 ttgggccacg ccaaatctcc tttttcgggc caccggatat aggggaacgg accggcgttt
    11281 accagcgacg caaccgc
//
LOCUS       DASWPU010000563         6017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_102518,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000563 DASWPU010000000
VERSION     DASWPU010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102518"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VGC27_11895"
     CDS             complement(<1..282)
                     /locus_tag="VGC27_11895"
                     /inference="COORDINATES: protein motif:HMM:NF017277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEY0283312.1"
                     /translation="MAGHEVEVSRGDDLLKLASDIVAAYVSNNPVPVSELPAMIKSVH
                     ATLGSLTGGGAGEVVTSQKPAVTVKKSVTPEYLICLEDGKKLKMLKRYLR"
     gene            674..1972
                     /gene="glyA"
                     /locus_tag="VGC27_11900"
     CDS             674..1972
                     /gene="glyA"
                     /locus_tag="VGC27_11900"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY0283313.1"
                     /translation="MTAVASPAVSSGFFNLGLAGADPDLARAIHDELIRQQEKIELIA
                     SENIVSRAVLEAQGSVLTNKYAEGYPGRRYYGGCEYVDVAEQLAIERACKLFDCSFAN
                     VQPHSGAQANQAVFFALLQPGDTFMGLSLAAGGHLTHGSPVNQSGKWFKPVSYTVRRH
                     DHRIDMDEVEALAKEHKPKLIIAGGSAYPRFIDFARFRQIADEVGAIFMVDMAHFAGL
                     VAGGVHPNPLDHAHVVTTTTHKTLRGPRGGMILTRDEALGKKINSAVFPGLQGGPLMH
                     VIAAKAVAFGEALKPEFKLYAKAVVENAKALAETLHAQGLDIVSGGTDTHLMLVDLRP
                     KGATGRAAEKALDRAFITCNKNAVPFDTEKPAVTSGIRLGSPAGTTRGFGAAEFREVG
                     KLIVEVVDALGKKGEDGDADVEAGVRERVRVLCNRFPIYR"
     gene            1982..2449
                     /gene="nrdR"
                     /locus_tag="VGC27_11905"
     CDS             1982..2449
                     /gene="nrdR"
                     /locus_tag="VGC27_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY0283314.1"
                     /translation="MRCPFCGHDDTQVKDSRPSEDNSAIRRRRHCPECGGRFTTFERV
                     QLRELIVIKKNNRREPFDRDKLERSINHALRKRPVERERVDRMITGIVRRLESMGENE
                     IPSQMIGELVMQALSSLDKVAYVRFASVYKNFRETKDFENLIGELEGDDEVKD"
     gene            2501..2944
                     /locus_tag="VGC27_11910"
     CDS             2501..2944
                     /locus_tag="VGC27_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEY0283315.1"
                     /translation="MSDIDKPLDDITGEIVDAAFMLHTGLGPGLLESVYETVLARELL
                     RRGLQVERQKPVSFHYDGLDFYDGFRIDLMVESRVVVEIKSVERLAPVHSKQLLTYLR
                     LLNLSVGLLINFGAPTLKEGLHRIVNGLSRTASPRLRVNQVPKDR"
     gene            2980..4068
                     /gene="ribD"
                     /locus_tag="VGC27_11915"
     CDS             2980..4068
                     /gene="ribD"
                     /locus_tag="VGC27_11915"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY0283316.1"
                     /translation="MTDLHHMRHALALAERALGIVAPNPAVGCVIVSREGYVAGRGWT
                     GRGGRPHAETVALAQAEEAARGATAYVTLEPCSHFGQTPPCANALAEAGIARVVAAVE
                     DPDPRVRGKGFELLREADVDVATGICAKQAAELNAGFFMRVRQGRPLVTLKIAQSLDG
                     KTATAMGESKWITGEAARAYGHLLRARHDAILVGIGTVLADDPLLTCRLPGLEDRSPL
                     RVVLDTRLKLNEWSKLAQSAGETPTLVFTASGEGGALAACGVEVVKTARDARGRPDLA
                     AVLAELGRRGITRLLVEGGATVHAAFLDRGLADRLEIFRSPLVLGAAGHDGIDSLAAL
                     TLGEAPRFRLTGRRSFGRDLLESFARED"
     gene            4078..4671
                     /locus_tag="VGC27_11920"
     CDS             4078..4671
                     /locus_tag="VGC27_11920"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY0283317.1"
                     /translation="MFTGLVSDIGRVRQVEKRGDTHLVIATHYDVSAVDAGASVACAG
                     ICLTVVDKGTAKDRWFAVTASGETLSKTTMGGWKKGTFVNLERPLRVGDEFGGHIVTG
                     HIDGIADVVSVKPEGESVRMVFKAPTPLARFIAAKGSVALDGVSLTVNETKGARFGVN
                     IIPHTSEVTTFGRLKPGDKVNLEIDIFARYVARLVKG"
     gene            4676..5788
                     /gene="ribB"
                     /locus_tag="VGC27_11925"
     CDS             4676..5788
                     /gene="ribB"
                     /locus_tag="VGC27_11925"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY0283318.1"
                     /translation="MFRDYISSIEEVIEEARNGRMFILVDAEDRENEGDLFIPAQMCT
                     PEAINFMARYGRGLVCLALTQERADELQIPMMTQVNSTPHKTAFTVSIEAREGVSTGI
                     SAHDRAHTVSVAIDSTKGPRDIVMPGHVFPLVARPGGALVRAGHTEAAVDISRLAGLN
                     PSGVICEIMNDDGTMARLPDLVQFAQLHGLKIGTISDLIAFRLKYDHFIRRAGETVFT
                     SHNGGEWKMIVYVNTLEYAEHIALVKGDLSLPEPALVRMHAINVLSDVLDDAHGPRDR
                     LGESMRIVAYKGRGVVVVLRQARATFVSDIVLQHVADDFDRRRVKESGVGAQILRDLG
                     VKDMILLTDTPEKKVVALDGYGLNIVGTHPISRKDA"
     gene            5792..>6017
                     /locus_tag="VGC27_11930"
     CDS             5792..>6017
                     /locus_tag="VGC27_11930"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY0283319.1"
                     /translation="MTPNILIVEAPFYPHISRLLLDGATAALERGGAYFDSVSVTGAL
                     EIAPVIHFAVKGGEHGGKGYDGFVALGCVIR"
BASE COUNT         1131 a         1872 c         1913 g         1101 t
ORIGIN      
        1 gcgcaggtag cgcttgagca ttttgagctt cttgccgtct tccagacaga tcagatattc
       61 cggcgtcacg gatttcttga ccgttacggc gggtttctgc gacgtgacca cttcgccggc
      121 gccgccgccc gtgaggctgc ccagcgtcgc atggacgctc ttgatcatcg cgggaagttc
      181 actgaccggt accggattat tgctgacata ggcagcgacg atgtcgctgg ccaatttgag
      241 gagatcgtcg cctcttgaaa cttccacttc gtgaccagcc attctattgg gccctctgtt
      301 caaggtaaaa acgccgcact ctgtttcgtc tgcggtatta cctttgttcc cagcaaaatc
      361 aagatattcc tctgcgacga attggggtca cgcgcaaatg acaacaataa tcgcgcgatc
      421 cgacacttgt ggatgtgtcc atcgtctcta ttgtatgttt tcagctgttg gttgtgaata
      481 cttgcgtgta aaatattgtt gctcaattaa tggttgtgtg tacaaatagc aaacctgccc
      541 ctcagtctga ctgtgacgga tgcccgcctc gcctcactgg cgctagattt ggtaaaggga
      601 taggggtacc acccccgacc ggtgcgacgg ccgacaaagg ctagcgccag gggacgtttc
      661 gagcgaggtg gttatgacgg cggtggcaag tcctgcggtg agttcgggtt tctttaatct
      721 tggattggcc ggggccgatc ctgatctggc ccgcgccatt cacgacgaat tgatccgcca
      781 gcaggagaaa atcgagctga tcgcctccga gaacatcgtc agccgggcgg tgctcgaagc
      841 gcaggggtcc gttctcacca acaaatacgc ggaaggctat cccggccggc gctattacgg
      901 cggctgcgaa tatgtggacg tcgccgagca actcgccatc gagcgcgcct gcaagctgtt
      961 cgattgcagc ttcgccaatg tgcagcccca ttccggcgcc caagcgaacc aggccgtgtt
     1021 cttcgcgctg cttcagcccg gcgacacctt catgggcttg tcgctggcgg cgggcggtca
     1081 tctgacgcac ggctcgccgg tgaaccagtc gggcaaatgg ttcaagccgg tgtcctatac
     1141 ggtgcgccgc catgaccacc gcatcgacat ggacgaagtg gaagccctag ccaaagaaca
     1201 taaaccgaag ctcatcatcg cgggaggctc cgcttatccg cgcttcatcg atttcgcccg
     1261 cttccgccaa atcgccgacg aagtgggcgc gatcttcatg gtggacatgg cgcatttcgc
     1321 gggcctggtg gcgggcggcg tgcatcccaa tccgctggac cacgcccatg tggtgaccac
     1381 caccacgcac aagaccctgc gcggcccgcg cggcggcatg attctcacgc gcgacgaggc
     1441 cctgggcaaa aagatcaatt cggcggtgtt tccgggattg cagggcggcc ctctgatgca
     1501 tgtgatcgcg gccaaggccg tggccttcgg cgaagcgctg aagccggagt tcaagctcta
     1561 tgccaaggcc gtggtggaga acgccaaagc gctggccgag acgctgcatg cgcagggcct
     1621 cgacatcgtt tcgggcggca ccgacacgca tctgatgctg gtcgatctgc gccccaaggg
     1681 cgcgaccggg cgcgccgccg agaaggcgct cgaccgcgcc ttcatcacct gcaacaagaa
     1741 cgccgttccc ttcgacaccg aaaagccggc ggtcacctcc ggcatcaggc tgggttcgcc
     1801 ggccgggacg acgcgcggct tcggggcggc cgaattccgc gaagtcggca aactgatcgt
     1861 cgaagtcgta gacgccctcg gcaagaaggg cgaggacggc gatgcggacg tcgaagccgg
     1921 cgtgcgcgaa cgcgtacgag ttctctgcaa tcgtttcccc atctaccgat aagggattcc
     1981 catgcgctgc cccttctgcg gtcacgacga cacccaggtc aaagacagcc gtccgagcga
     2041 ggacaattcc gccatccgcc gccgccgtca ttgtcccgaa tgcggcgggc ggttcaccac
     2101 cttcgagcgc gtgcagctgc gcgagctgat cgtgatcaag aagaacaacc ggcgcgagcc
     2161 cttcgaccgc gacaagctgg aacgctcgat caaccacgcc ctgcgcaagc gcccggtcga
     2221 gcgcgagcgc gtcgatcgca tgatcaccgg catcgtgcgc cggctggaaa gcatgggcga
     2281 gaacgaaatc ccctcgcaga tgatcggcga gctggtgatg caggcgctgt cgagcctcga
     2341 caaggtggcc tatgtgcgct tcgcctcggt ctacaagaat ttccgcgaga ccaaggattt
     2401 cgaaaacctg atcggcgagc tggaaggcga cgacgaggtg aaggactgaa cgtttcgctg
     2461 ccgtgattaa gcacgcggag gcgcggagta ggcggagcgg atgagcgata tcgataagcc
     2521 cttggacgac atcaccggcg agatcgtcga tgccgccttt atgctgcaca caggtctcgg
     2581 tccggggttg ctggaatccg tttatgaaac agtgctggcg cgtgaattgc tgcggcgagg
     2641 gttgcaggtc gagcgccaga aaccggtttc gttccattac gacggattgg atttctacga
     2701 cggctttcgg atcgacctta tggtcgaatc gcgagtagtt gttgagatca agtcggttga
     2761 gaggctggca ccggtacatt ccaagcaatt actcacctat ctgcgactgt tgaacctttc
     2821 tgttggattg ctgatcaatt ttggcgcgcc gacgctgaag gaaggattgc accggatcgt
     2881 aaatggcttg tcgcgaaccg cgtctccgcg tctccgcgtg aaccaagtgc caaaggaccg
     2941 atagatccgc actatgtcgg ccatgatgat gagcaaacgg tgaccgatct ccatcatatg
     3001 cgccatgcgc ttgcgctggc ggaacgcgcg ttggggatag tcgcgcccaa ccccgccgtc
     3061 ggttgcgtga tcgtgtcgcg cgaaggttat gtcgccggcc gcggctggac cgggcggggc
     3121 gggcggcccc atgccgagac cgtcgccttg gcgcaagcgg aggaggcggc acgcggcgcc
     3181 acggcctatg tcacgctcga accttgttcc catttcggcc agacaccacc ttgcgccaac
     3241 gcgctggcgg aagcgggcat cgcgcgcgtc gtcgcggcgg tggaagaccc cgatccgcgc
     3301 gtccgcggca aaggcttcga attgctgcgc gaagcggacg tggatgtcgc aaccggaatc
     3361 tgcgccaaac aggccgccga actgaacgcc ggtttcttca tgcgcgtgcg ccaaggacgg
     3421 ccgctggtca ccctgaagat cgcccagagc ctcgacggca agacggctac cgcgatgggt
     3481 gaaagcaaat ggatcaccgg cgaggcggcg cgcgcctatg gccatctgct gcgcgcgcgc
     3541 cacgatgcga tcctggtcgg catcggtacc gtcttggccg acgatcccct gctcacctgc
     3601 cgcttgccgg gactggaaga ccgttcgccg ctgcgcgtcg tgctggatac aaggctgaaa
     3661 ctgaacgagt ggtccaagct ggcccagagc gccggcgaaa cgccgaccct ggttttcacc
     3721 gcctccggcg aaggaggggc gctcgccgct tgcggggtcg aagtcgtgaa gaccgcacgc
     3781 gacgcccgcg gccggcccga cctggcggcg gtcctcgcgg aattgggcag gcgcggcata
     3841 acgcgccttc tggttgaagg cggtgcgacg gttcatgcgg cctttctgga tcgcggtctg
     3901 gccgaccggc tggaaatctt ccgcagtccg ctcgttttgg gcgcggccgg ccacgacggg
     3961 atagactctc tggctgcctt gaccctggga gaagcaccgc gcttccgtct cacggggcgc
     4021 cggagcttcg gccgggacct gctggaaagc ttcgcgcgcg aggattagaa ggaaaccatg
     4081 ttcacaggac ttgtcagcga tatcggccgg gtgcgccagg tggaaaagcg cggcgacacg
     4141 catctcgtga tcgccacgca ttacgatgtg tcggcggtgg atgcgggcgc ctcggtcgcc
     4201 tgcgccggaa tatgtctgac ggtcgtggac aagggcaccg ccaaggaccg ctggttcgcc
     4261 gtcacggcgt cgggcgagac cctgtccaaa acgaccatgg gcggctggaa gaagggcacc
     4321 ttcgtcaacc tcgaacggcc gttgcgcgtc ggcgacgagt tcggcggcca tatcgtgacc
     4381 ggacacatcg acggcatcgc cgatgtcgta agcgtgaagc cggaaggcga atccgtccgc
     4441 atggtcttca aggcgccgac cccgctggcg cgcttcatcg ccgcaaaagg ctccgtcgcg
     4501 ctggacggcg tttccctgac cgtgaacgaa accaagggcg cgcgcttcgg cgtcaacatc
     4561 attccgcaca cctcagaggt gacgaccttc gggcgcctga agccgggcga caaagtcaat
     4621 ctggaaatcg atattttcgc ccgctacgtc gcccgcctcg tgaaaggctg accgcatgtt
     4681 ccgcgattac atctcttcga tcgaggaagt gatcgaagaa gcccgcaacg gaagaatgtt
     4741 catcctggtg gacgcggagg accgcgagaa cgaaggcgac cttttcatcc cggcgcagat
     4801 gtgcacgccg gaagcgatca acttcatggc caggtacggc cgcgggctgg tttgcctggc
     4861 gctgacgcag gagcgcgccg acgaattgca gattccgatg atgacgcagg tgaactcgac
     4921 gccgcataag acggcgttca ccgtgtccat cgaggcgcgc gagggcgtct ccaccggcat
     4981 ttcggcgcac gaccgcgccc ataccgtctc ggtcgccatc gattccacca aggggccgcg
     5041 cgatatcgtc atgcccggcc acgttttccc gctggtcgcc cggcccggcg gcgcgctggt
     5101 gcgcgccgga cacaccgaag ccgcggtcga tatctcccgg ctcgccggtc tcaacccctc
     5161 gggcgtgatc tgcgagatca tgaacgacga cggcaccatg gcgcggctgc cggacctggt
     5221 gcagttcgcc caattgcacg ggctgaagat cggcaccatt tccgatctca tcgccttccg
     5281 cctcaaatac gatcatttca tccgccgcgc cggagagacg gtgttcacca gccacaacgg
     5341 cggcgaatgg aagatgatcg tctacgtcaa cacgctcgaa tatgccgagc atatcgcgct
     5401 ggtgaagggc gatctgtcgt tgccggaacc ggcgctggtg cgcatgcacg ccatcaacgt
     5461 cctcagcgac gtgttggacg acgcccacgg cccgcgcgac cggctcggcg agtccatgcg
     5521 gatcgtcgca tacaaaggcc gcggcgtcgt cgtcgtgctg cgccaggcgc gcgccacctt
     5581 cgtgtccgac atcgtgctgc aacacgtggc ggacgatttc gaccgccgcc gcgtgaagga
     5641 gtccggcgtg ggtgcgcaga tacttcgcga cctcggcgtc aaggacatga tccttctcac
     5701 cgacacgccg gagaaaaaag tcgtggcgct cgacggctac ggcctcaaca tcgtcgggac
     5761 gcatcccatc agccgaaagg acgcctaaaa tatgacgccg aatatcctga tcgtggaggc
     5821 gccgttctat ccgcacatct cgcgcctgct gctggacggc gcgacggcgg cgctcgaacg
     5881 gggcggcgcc tatttcgaca gcgtctccgt caccggggcg ctggagatcg cgccggtcat
     5941 tcatttcgcg gtcaagggcg gcgagcacgg cggcaagggc tatgacggtt tcgtggcgct
     6001 gggctgcgtc attcgcg
//
LOCUS       DASWPU010000564         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_53721,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000564 DASWPU010000000
VERSION     DASWPU010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53721"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VGC27_11935"
     CDS             <1..668
                     /locus_tag="VGC27_11935"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HEY0283320.1"
                     /translation="LKSWERLPDLVTHSGQQRNVVLHPEFVNRKYALYTRPQDGFITV
                     GSNGGIGWGLTDDMTRAEIGEESVIDGKVYHTVKEVKNGQGPAPLKTPQGWLHLAHGV
                     RNTAAGLRYVLYMFMTALDRPWVVIHAPGGHFLAPAGEERVGDVSNVVFSNGWVVNEK
                     GEVFIYYASSDTRLHVATSSLERLVDYVSATPPDPLTSAACVQQRRKLIEKNLALLGH
                     DTL"
     gene            844..>2005
                     /locus_tag="VGC27_11940"
     CDS             844..>2005
                     /locus_tag="VGC27_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283321.1"
                     /translation="MHLQLIDIIIIVAFLAASVVVGYWVSHRASRDTKSYFLGGNVLP
                     WYMLGISNASGMFDIAGTMLLVTWLFIYGVKSVWIPWLWPVFNQVFLMVYLSGWLRRS
                     GVTTGAEWIRFRFGNGLGAQLSHLIVVVFALVSVIGFLSYGFKGIGKFAVDFLPWNFS
                     ADPSANENIYALILIGITTFYVVKGGMFSVVITEVMQFCLLSIAAIAIGLIAMSQVSP
                     ETLRSVVPVGWDNIFFGWNTGLDWSKHIPAANDKIIKDGFSLFGFLWMMLLFKGYIVA
                     AAGPAPNYDMQRVLSTRNPREACMMSAVVNVVLNVPRYFMVAGLAILALAFYNSDIRA
                     MGVDMDFEKVLPDALSRFVPVGLLGITVAGLLAAFMSNLAATVNAAPPYIVND"
BASE COUNT          360 a          628 c          593 g          424 t
ORIGIN      
        1 atctcaaatc gtgggagcgg ctgcccgatc tggtgacgca ttcgggccag cagagaaacg
       61 tcgtgctgca tcccgaattc gtcaaccgga aatacgccct ctacacccgc cctcaagacg
      121 gtttcatcac tgtcggcagc aacggcggga tcggctgggg cctgacagac gacatgacgc
      181 gggccgagat cggcgaggag agcgtcatcg acggcaaggt ctatcacacc gtcaaggaag
      241 tgaagaacgg ccagggcccg gcgccgctca agacgccgca gggctggctg catctggcgc
      301 atggcgtgcg caatacggcg gcgggcctgc gctatgtcct ttatatgttc atgacggcgc
      361 tcgaccgccc ctgggtggtg atccacgcgc cgggcgggca tttcctcgcg ccggcaggcg
      421 aggagcgcgt gggcgacgtc tccaatgtgg tgttctcgaa cggctgggtc gtcaacgaga
      481 agggcgaggt gttcatctac tatgcctcct ccgacacgcg cctgcatgtc gcaacctcca
      541 gcctcgaacg cctggtcgat tacgtgagcg caacgccgcc cgatccgctg acctccgcgg
      601 cctgcgtgca gcaacgccgc aaactgatcg aaaaaaactt ggctttgctt gggcatgaca
      661 cgttgtgagc gttaacacgg ccggtgaaac cagggcgccc cggatgtgtt tcgatgcgcc
      721 agattcgcgc gctccagccc aactgtgccg ccccggcttg acagcgctgt caccatgaaa
      781 gaatgtctgc gcgagaggaa ctgcatacaa taccaacgat aaccaggaat gccggggggt
      841 caggtgcatc tacagctcat cgacatcatc atcatcgttg cctttctggc ggcgtccgtc
      901 gtcgtcggat actgggtttc gcaccgcgcc tcgcgcgaca ccaaatccta tttcctcggc
      961 ggcaacgtgc tgccctggta catgctcggc atctcgaacg cctcgggcat gttcgacatc
     1021 gcgggcacta tgctgctcgt tacctggctc ttcatctacg gcgtgaagag cgtctggatt
     1081 ccctggttgt ggccggtctt caaccaggtt ttcctgatgg tctatctctc gggctggctg
     1141 cgccgctcgg gcgtgaccac gggtgccgaa tggatccgct ttcgttttgg caacgggctc
     1201 ggagcccaac tctcgcacct gatcgtggtg gtcttcgcgc tggtcagcgt catcggcttc
     1261 ttgagttacg ggttcaaggg catcggcaag ttcgcggtcg atttcctgcc ctggaacttc
     1321 tctgcggatc cttcggccaa cgaaaatatc tacgccctca tcctcatcgg catcacaacc
     1381 ttctacgtgg tgaagggcgg gatgttcagc gtggtgatca ccgaggtcat gcagttctgc
     1441 ttgttgtcga tcgccgctat tgccatcggc ctcatcgcca tgagccaagt gtcgcccgaa
     1501 acgctgcgca gcgtcgtgcc tgtcggctgg gacaacatct tcttcggctg gaacaccgga
     1561 ctcgactggt ccaaacatat ccctgcggcc aacgacaaga tcatcaagga cggcttttcg
     1621 ctgttcggat tcctatggat gatgctgctt ttcaagggct acatcgtggc ggcggcaggg
     1681 cccgcgccca actatgacat gcaacgcgtc ctctcgacgc gcaatccgcg cgaagcctgc
     1741 atgatgagcg cggtggtcaa cgtcgttctc aacgtgccgc gctacttcat ggtggcgggc
     1801 ctcgccattc tggcgctcgc cttttacaac agcgatatcc gcgccatggg cgtcgacatg
     1861 gacttcgaga aagtgttgcc cgacgcactt tcccgtttcg tgcccgtggg cctgctcggc
     1921 atcaccgtag cgggcctgct ggcggccttc atgtcgaatc ttgctgccac ggtgaacgcg
     1981 gcaccgccct atatcgtcaa cgaca
//
LOCUS       DASWPU010000565         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_23754,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000565 DASWPU010000000
VERSION     DASWPU010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23754"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="VGC27_11945"
     CDS             complement(<1..1165)
                     /locus_tag="VGC27_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283322.1"
                     /translation="MKRLIAGVVCAFALLLASSAQAAPEGKYKNFKVAIYVVVNATKR
                     LADPEVFAAEYARASAQLKFDKVYIEVYRNRLFATDEEIEKVKAAFLAKGVAVAGGIT
                     PAAGGADGQFGTFDYEDEKDRAECRKAAELAAKHFDQVILDDFFFYTSKTEADIAAKG
                     GRSWTEYRLAAMREASKSLILDPAHAANPRAKVVIKYPNWYEHFQGLGYDLEQQSQMF
                     DGIYTGTETRDPTLTDQLLQQYESYQIIRYYDAIRPGGNGGGWVDTYSLRYVDRYAEQ
                     LWDTIFAKAPEITLFNWADLIREEQVPAGERPWAPLKTSFDWAAASKPFPGNVPAGWG
                     KAAGVALEAVDRVAGHLGNPVGIAAYKPYQSSGEDFLQNYLGNLGLPLAMTPEF"
BASE COUNT          332 a          523 c          555 g          360 t
ORIGIN      
        1 ggaattccgg cgtcatcgcc aggggcagcc cgagattgcc gagatagttc tgcaaaaagt
       61 cctcgcccga agactggtag ggcttgtaag cggcgatgcc gaccggattg ccgagatggc
      121 ccgccacgcg gtccaccgct tccagcgcca cgcccgcggc cttgccccag ccggccggca
      181 cattgccggg gaagggcttc gacgccgcgg cccaatcgaa gctggtcttg agcggcgccc
      241 agggccgctc gcccgccggc acctgttcct cgcggatcag atcggcccag ttgaacagcg
      301 tgatctccgg cgccttggcg aagatcgtgt cccacaattg ctcggcatag cgatcgacat
      361 agcgcagcga ataggtatcg acccagccgc cgccgttgcc gcccggacga atggcgtcgt
      421 agtagcggat gatctggtag ctctcatatt gctgcaaaag ctggtcggtc agcgtcgggt
      481 cgcgcgtctc ggtgccggtg tagatgccgt cgaacatctg cgattgctgt tcaaggtcgt
      541 agcccagccc ctgaaaatgt tcgtaccagt tgggatattt gatgacgacc ttggcgcgcg
      601 ggttcgccgc atgggcggga tcgaggatga gggatttcga cgcttcgcgc atcgccgcca
      661 ggcgatattc ggtccaggag cggccccctt tggcggcgat gtcggcctcg gtcttcgagg
      721 tgtagaagaa gaaatcgtcg aggatcacct gatcgaaatg cttggccgcc agttccgccg
      781 ccttgcggca ttcggcacgg tccttctcgt cctcgtaatc gaaggtgccg aactggccgt
      841 ccgcgccgcc cgccgcaggc gtgatgccgc ccgccaccgc cacgcccttg gcaaggaacg
      901 ccgccttgac cttctcgatt tcctcgtcgg tggcgaaaag gcggttgcgg tagacttcga
      961 tataaacctt gtcgaacttc aactgcgcgc tggcgcgggc atattcggcg gcgaacactt
     1021 ccgggtccgc cagccgtttg gtggcgttga cgacgacgta aatcgccacc ttgaagttct
     1081 tgtacttgcc ttcgggcgcc gcctgggcgc tggaggcgag aagcaaggcg aaagcgcaaa
     1141 caactccggc gatcaggcgt ttcatgtctc ctcccgagaa attttaatcg gtcttcctcg
     1201 cctccttggg cgatatcggc cggggcgcag ccgggcattg cgacgggccg ttcgccggct
     1261 cgacgcgcag gcattgtgcc acaagacgcc agcttcgcgt tctctgaatg cggcgctggc
     1321 cgagggggcg gccgcggaag gtgctgtggg cgaggcccgt aaatggctga aaacagacgg
     1381 cttcccggag ccgaattccg tccgtcgagg ttcggtcggt tccgcgccgg agggagccat
     1441 aagagagacg taatatcaag taattatagt gaaaaaacta ttttgaatct ggcagatgcc
     1501 ctcaaatata cccccaactt tggagttgtc gtctatattt atctgtttcc aaacgtcact
     1561 gcgctaactc aaggcatgcc tgccattatg tactcgctcc aaggcacggt ggcttgaggg
     1621 aggtggacaa acaatcaagg acgcgcatat ctcagtttgg aatgaggggc tggttccatg
     1681 actgatggtc ggctggtttt ggaatacgtt gtcgattatt ggggcaaggc gcagccgaca
     1741 gtcagcgagg gccccaaatg gcacccactt
//
LOCUS       DASWPU010000566         1982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_84829,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000566 DASWPU010000000
VERSION     DASWPU010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84829"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VGC27_11950"
     CDS             <1..231
                     /locus_tag="VGC27_11950"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEY0283323.1"
                     /translation="GVVAFVAVADGKASLVVGVTDDLKDRFSAVDLVRLGAEALGGKG
                     GGGRPDLAQAGGPDGTRAADALAAIEARLAAK"
     gene            complement(338..718)
                     /locus_tag="VGC27_11955"
     CDS             complement(338..718)
                     /locus_tag="VGC27_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0283324.1"
                     /translation="MIGYVTIGALDSEASGKFYDAVLGALGEERKFADGGWIGYGPKG
                     EDSHYAYVCPPFDGKPARAGNGIMLAFKGHSKEEIDAAYAAGLANGGSDEGAPGFRPP
                     EGKEFYGAYLRDPTGNKICVYFKT"
     gene            complement(1167..>1982)
                     /locus_tag="VGC27_11960"
     CDS             complement(1167..>1982)
                     /locus_tag="VGC27_11960"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY0283325.1"
                     /translation="HAFGDQYKATDFTFPGKGTLSIKFVGDDGKVIEHEVFRAPGSGV
                     AMAMYNLDESIRDFARASMNYALARKYPLYLSTKNTILKAYDGRFKDLFAEVFEAEFA
                     AKFKAAGITYEHRLIDDMVASALKWSGGYVWACKNYDGDVQSDTVAQGFGSLGLMTSV
                     LMTPDGKIVEAEAAHGTVTRHYREHQKGKPTSTNSIASIFAWTRGLAHRAKLDDNAAL
                     ATFAATLEKVCVDTVESGFMTKDLALLVGSEQKWLTTEAFLDKVDENMKKAMG"
BASE COUNT          389 a          585 c          617 g          391 t
ORIGIN      
        1 ggcgtggtgg ctttcgtggc ggtggccgac ggcaaggcgt cgctggtcgt cggcgtcacc
       61 gacgatttga aggaccgttt cagcgccgtc gatttggtgc ggctgggcgc cgaagcgctg
      121 ggcggcaagg gcggcggcgg acggcccgac ttggcgcaag cgggtgggcc ggacggaacg
      181 cgcgccgctg acgcgctggc ggcgatcgaa gcgcggctgg ccgccaagta gagtatcttc
      241 ggaacagact gagcaactca actcgtcatg gccgcctcaa gggcggccat gacgaattaa
      301 caagacatga tccagttcaa aaccgctcca gcgtcggtca cgtcttgaag tacacgcaga
      361 tcttgttgcc ggtgggatcg cgcaaatacg cgccgtaaaa ttccttgcct tccggcggac
      421 ggaagccggg cgccccttcg tcgctgccgc cattggccag acccgcagca taggcggcgt
      481 cgatctcttc tttggaatgg cccttgaaag ccagcatgat gccgttgccg gcgcgtgccg
      541 gcttgccgtc gaacggcggg cagacatagg cgtaatgact gtcctcgccc ttggggccgt
      601 agccgatcca gccgccgtcg gcgaatttgc gctcctcgcc cagcgcgccc agaaccgcat
      661 cgtagaattt gcccgacgcc tcgctgtcga gcgcgccgat ggtgacgtaa ccgatcataa
      721 aacccctccc gcaaatgctt gccaggacac ctagcttaat gacaagaaca aatcaagaac
      781 atattggaag gcattcacat ggcgtttgac agataagccg atgggcacgc gtccgatttc
      841 cgtcgaggtg tctgtgttaa atgtggaatg actcgtgaag cttacgagga taatggcagt
      901 cccccttgtc aggtagaaca tctctcttca gaaagactga agaagggcgg gatgccaatc
      961 gtcgaagatg gcgattagta agctcagtgt cacggcccgc aagtccgcag cggcagggtg
     1021 cgaacgggac gcccaggtga cacatcgcgg gtttcaaagc cgacactgag attacttgtc
     1081 tcaatttgcg cgcgtcatca cctggatggc ctgcatccgc aggccatgac agcagggtta
     1141 agtgcgacgg ccgagccgaa atagcgtcac cccatcgcct ttttcatatt ttcgtccact
     1201 ttgtcgagga aggcttccgt cgtcagccat ttctgctcgg agccgacgag cagcgcaagg
     1261 tccttggtca tgaagccgga ctccaccgta tcgacgcaaa ctttttccag agtggcggcg
     1321 aatgtcgcca gcgccgcgtt gtcgtccagc ttggcgcggt gggcgaggcc ccgcgtccag
     1381 gcgaaaatgg acgcgatgga attggtggag gtgggtttgc ccttctgatg ttcgcggtaa
     1441 tggcgcgtca cggtgccgtg tgcggcctcg gcttccacaa tcttgccgtc cggcgtcatc
     1501 agcacggatg tcatcagccc cagcgaaccg aagccttgcg ccaccgtgtc ggactgcacg
     1561 tcgccgtcgt aattcttgca ggcccagacg taaccgccgg accatttgag ggcgctcgcc
     1621 accatgtcgt cgatcaaacg atgctcgtag gtgatgccgg ccgccttgaa cttcgccgca
     1681 aactcggcct cgaacacctc ggcgaacagg tccttgaagc gcccgtcata agctttgagg
     1741 atcgtgttct tggtggagag atagagcggg tatttgcgcg ccagcgcgta gttcatcgaa
     1801 gcgcgggcga aatcgcggat ggattcgtcg agattgtaca tcgccatcgc cacgcccgaa
     1861 ccgggcgcgc gaaacacttc gtgctcgatc accttgccgt cgtcgccgac gaatttgatc
     1921 gacagcgtgc ccttgccggg gaaggtgaaa tcggtcgcct tgtactgatc gccgaaggcg
     1981 tg
//
LOCUS       DASWPU010000567         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_47933,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000567 DASWPU010000000
VERSION     DASWPU010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47933"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VGC27_11965"
     CDS             complement(<1..311)
                     /locus_tag="VGC27_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283326.1"
                     /translation="MAKYERDSHEPDDVRVFDGGGDDEDEGGSHLPVVIVISLLVLAA
                     FGGVVWLAYNNGVARGRADVPVSVAAQNTPSEPPAVAPGSGAVPGKQIKIYQQQAGSE
                     E"
     gene            complement(382..1608)
                     /locus_tag="VGC27_11970"
     CDS             complement(382..1608)
                     /locus_tag="VGC27_11970"
                     /inference="COORDINATES: protein motif:HMM:NF002328.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY0283327.1"
                     /translation="MASARAATAQNPPAGRLLGPIYSPPAARAPFATKPENSRGRLYP
                     EQESATRTCYQRDRDRILHSTAFRRLKHKTQVFVQSEGDYYRTRLTHSLEVAQIARTT
                     ARVLALDEDLAEAVSLAHDLGHTPFGHAGEEALAAASAEIGGFDHNAHGLRLVTKLEH
                     RYAGFDGLNLTWETLEGLVKHNGPIAEPVPGPIAGFDARWPLDLSTWPGLEAQIAALA
                     DDIAYLTHDIDDGLRAGLFGIGDLAKAPLAGAPVEAIRKRYGELELSRFIGELVRTLI
                     GAMTADLLAETGRILLHTQPGSAAEVRAARGPAARFSPEMAGEVAALRQFLFVRMYRH
                     LRVMSSMDKAKMVVEELFGALLREPSLLPADWAAGCGQAGDGATARVVRDYIAGMTDR
                     YALQEYRRIFHSEIAL"
     gene            1659..2003
                     /locus_tag="VGC27_11975"
     CDS             1659..2003
                     /locus_tag="VGC27_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020056313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY0283328.1"
                     /translation="MSTETALPVTVSPRAAKRIAEIIAAEGRPAMLRLAVAGGGCSGF
                     QYTYAIVPEAAADDLVLAEAGAKVAIDQVSLGYLLGSKVDFVDDLMGRAFKIQSPAAT
                     ASCGCGTSFSLD"
BASE COUNT          339 a          677 c          639 g          351 t
ORIGIN      
        1 tcctcctccg acccggcctg ctgttgatag attttgatct gtttgccggg aacggcaccg
       61 ctgccgggcg cgaccgccgg cggctcgctg ggagtatttt gcgccgccac ggataccgga
      121 acatcggcgc gtccgcgcgc cacgccgttg ttgtaggcaa gccagaccac gccgccgaaa
      181 gccgccagca ccagcagcga tatgacgatc acgaccggaa gatgcgaacc accctcatcc
      241 tcgtcgtccc cgccgccgtc gaagacgcgc acgtcgtccg gctcatggct gtcgcgttcg
      301 tatttggcca tacgcaagct ccgaatctga acgcctcaat ctagcaccgg cctaccgccg
      361 aatcacggcg tccagccgct tctagagggc tatctcggag tggaatatgc gacgatattc
      421 ctgcagcgca tagcggtccg tcatgccggc gatataatcg cgcacaactc tcgccgtggc
      481 tccgtcgccc gcttggccgc agcccgccgc ccagtccgcc ggcagcagcg acggctcgcg
      541 cagcagcgcg ccgaacaatt cctccacaac catttttgcc ttgtccatgg aggacatgac
      601 gcgcaaatgc cggtacatcc gcacgaagag gaactgcctg agcgcggcga cctcgcccgc
      661 catctccggc gagaatctgg ccgccggccc gcgcgccgcc cgcacctcgg cggccgagcc
      721 gggttgtgta tgcagcagaa ttctgccggt ttcggccaac aggtcggcgg tcatggcgcc
      781 gatcagtgta cggacgagtt caccgatgaa gcggctgagt tccagttcgc cgtaacgctt
      841 gcggatcgcc tccacgggcg cgcccgccag cggcgccttg gccagatcgc cgatcccgaa
      901 cagaccggcg cgcagaccgt cgtcgatatc gtgggtgaga taggcgatgt cgtcggcaag
      961 cgcggcaatc tgcgcttcca gccccggcca agtcgaaaga tcgagcggcc agcgggcatc
     1021 gaagccggcg atcggccccg gcaccggctc ggcgatgggg ccgttgtgct tgaccaggcc
     1081 ctcaagcgtt tcccaggtca aattcagacc gtcgaatccg gcgtagcgat gttccagctt
     1141 ggtgaccagc cgcagcccat gggcgttatg gtcgaagccg ccgatctcgg cgctggccgc
     1201 cgccaaggcc tcctcgccgg catggccgaa cggggtgtgg ccgagatcgt gcgccaggga
     1261 caccgcttcg gcgaggtctt cgtccagcgc cagcacgcgc gcggtggtgc gggcgatctg
     1321 cgccacttcc agcgaatgag tcaggcgggt gcggtagtaa tcgccttcgc tctgcacgaa
     1381 gacctgcgtc ttgtgcttaa gcctacgaaa cgccgtggaa tgaaggatgc ggtcgcggtc
     1441 gcgctgatag caggtgcgcg tggcgctttc ctgctcgggg taaagacgcc cccggctatt
     1501 ctccggcttg gtcgcaaacg gcgcgcgcgc ggcgggcgga ctataaatcg gccccaaaag
     1561 ccgcccggcg ggcgggttct gggctgttgc ggctcttgcg cttgccattt ccaaccctac
     1621 attggactcg ctatgattat gccacgcaaa ggaagtccat gagtacggaa acggccctgc
     1681 ccgtcacggt ttctccccgc gccgccaagc ggatcgccga gataatcgcc gccgaaggca
     1741 ggccagcgat gctgcgcctg gcggtggccg ggggcggctg ctcgggcttc cagtacactt
     1801 atgcgatcgt gccggaggcg gccgcggacg accttgtgct cgccgaggcc ggcgccaagg
     1861 ttgccatcga ccaagtttcg ctcggctatc tgcttggcag caaggtcgat ttcgtcgacg
     1921 acctgatggg ccgcgccttc aaaatccaaa gtccggctgc gacggcctcc tgcggctgcg
     1981 ggacgagctt ttcgctcgat tgaata
//
LOCUS       DASWPU010000568         5128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_4701, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000568 DASWPU010000000
VERSION     DASWPU010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4701"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1489
                     /locus_tag="VGC27_11980"
     CDS             242..1489
                     /locus_tag="VGC27_11980"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEY0283329.1"
                     /translation="MAEPALNSKHDTLAPGEALRLLEQREQSSQHELASRTDAGEDVL
                     HYLAEHGAAATRRAVAANPAASPASNRLLADDEEDEVRVELARKIGRLFPGLLLAEKK
                     HLRDLTIETLERLARDEEPRVRAMLAEEIKHLDCVPKDVVQRLAHDAETVVCAPIIEY
                     SPLLSDTDLIEIVAVSHANAMLAAVARRKGLSGEVSDAVVATGDTTVIAILLANIDAR
                     LRKKTLDTIISRAAEVAEWHGPLVLRADLSPRAIRRIASFVASALIDLLAARQELDEA
                     TRTHLARKLRSRRQQETKTAGKPPASAAAEVEAACRAGKLDDAFVAAAAEAYQKDTVT
                     RALSVLAKTGETTVRRILDSGSAKAVTALCWRAGLSMRIAFKLQNTIMRLKGPDLLPA
                     RGGIDFPLSEDEMRWHLGYFGIV"
     gene            complement(1471..2232)
                     /locus_tag="VGC27_11985"
     CDS             complement(1471..2232)
                     /locus_tag="VGC27_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0283330.1"
                     /translation="MSVEVLAAEAVHFSRQSRAVLAGATLSLSSREIVCLLGRNGAGK
                     TTLLRIMLGFLHPERGIVRLDGRPLSGLSRRTLARRVAYVPQLHQPPFPYLVREVVGM
                     GRMPSTGLFGQISRSDREAVDTVMESLGITPLAGRRYTQLSAGERQLVMIGRALAQGA
                     RLLVMDEPFTGLDYGHQMELMERLRGLKGEGYGMLMTTHQPDHAAIIATRVTTLEAGR
                     IADDGMPEDIITPAVIRRLYGVEMHSFGCAQTIPK"
     gene            complement(2222..3310)
                     /locus_tag="VGC27_11990"
     CDS             complement(2222..3310)
                     /locus_tag="VGC27_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY0283331.1"
                     /translation="MSCSTLAEMERKQVRRHALRRIVAATGPPWELVLAAGLVLAMML
                     ALSVGKYPVAIGEIPRFFLASLGLCDMAADRYAALANVIIDIRLPRVLSAVLVGSALA
                     SSGAAFQAVFRNPLVSPGLLGVLSGASFGAALGIVLGVSWYVVQSLAFVMGLGAVVFG
                     VAVARLFGNAATLTLVLGGMVSGALFASLLSITKYIADPMNALPNIVYWLMGNLAQSN
                     LHQIAWLTIPMFGGVLLLGVLGRALDALAMGDDEARSLGVPVGVIRFAVIATATLISS
                     LTVSIAGMIGWIGLIVPHIVRLALGASNTRLMPSSALFGAMFLLGADYLARTLADTEI
                     PIGIVTELLGIPIFLLVLSRTSRGWNER"
     gene            complement(3307..4332)
                     /locus_tag="VGC27_11995"
     CDS             complement(3307..4332)
                     /locus_tag="VGC27_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0283332.1"
                     /translation="MAGTRRYGWALGLAAALAIAVAPAPAREIVDMSGRPVIVPDTIH
                     RAYAMTHAFPLITAIAPDLVAGFAMPVPPKPEMLEFLPPGMAGLPNIGGGADVNMEKL
                     KAVGIDVAFGWTTPKEMYQVKLLERINVPVVFIDVDRLSQYPGTFRFLGKLFHREARG
                     EELAKTLEVLVARLKKYAAAVPEKEKPRVYYAESIDGLVSQCEGTDRSEVIVLAGGRN
                     ALTCSKADLLADHYPIDLETLLTVDPDVIVTRFPATASAIKSDPRWARLRAVRDGKVF
                     AVPAYPFNWFDRPPSFMRALGAEWLGTKLHPKVYRIDLKAEIRRFHHVFFGTSLSDAQ
                     VEKLLSP"
     gene            complement(4344..>5128)
                     /locus_tag="VGC27_12000"
     CDS             complement(4344..>5128)
                     /locus_tag="VGC27_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0283333.1"
                     /translation="VTDIDDATSYTLGLSYKPRKNVTIYANYASSVNPDPGQAPSTAL
                     NAYDFLDPFESTQWEAGVKTELSGVEIDFALFHIERPTAWTGPDKIYKVQGQQRNRGA
                     EVMAKGKLSDDITLFGGFTWLDTKITGTDTEATDGKRAVGVPEWQGNLFTEYRLPQPF
                     AGVTASLNLHYTGERTANTYGTTTADGYATVDLGLRYTGDLPEGTISGKYTLRLSVNN
                     LFDERYWASLFPGSIDGSGTASGSTAFVGEPRTVKLTLSLLN"
BASE COUNT          973 a         1655 c         1658 g          842 t
ORIGIN      
        1 gtccgccgac gccatactgt cggggtgcgt cgcttggtag atcttcttgt acatcgcctt
       61 acctctatgc tgttcgcgcg tcccatgcgc cccttgattt ggtaaccggt gtcatacgcc
      121 caggaccaac acccgtgcaa cccgttttag agccgcagac ggcggtaaga ttccgtaaat
      181 caaacttttt caatcggtct taggagatgc ggcgccatgc ttccgttgta aggggaagaa
      241 attggccgaa cccgccctca attccaagca tgacactctg gctccgggcg aagccttgcg
      301 cctgttggag cagcgcgagc aatcgtccca gcacgaactg gcttcgcgca ccgatgccgg
      361 cgaggacgtt ctgcactatc tcgcggagca tggcgcggca gccacccgcc gggcggtcgc
      421 cgccaatccc gccgcctcgc ccgcatcgaa ccggctcctg gccgacgacg aggaagacga
      481 agtgcgcgtc gaactggcgc gcaagatcgg ccggctgttc cccggtcttc tgctcgccga
      541 gaaaaagcat ctgcgggatc tcacgatcga aacgctcgaa cggctcgccc gcgacgagga
      601 gccgcgcgta cgcgccatgc tggccgagga aatcaaacat ttggactgcg tgcccaagga
      661 cgtggtgcag cggctggccc acgacgccga gaccgtcgtt tgcgcgccga tcatcgaata
      721 ttcgccgctt ttgagcgata cggacctgat cgagatcgtc gccgtctccc acgccaacgc
      781 gatgctcgcc gcggttgccc ggcgcaaggg cctgtccggc gaggtcagcg acgcggtggt
      841 cgccaccggc gatacgacgg tcatcgccat cctgctggcg aacatcgacg cgcgcctgcg
      901 caagaagacg ctcgacacca tcatttcgcg ggccgccgag gtcgccgaat ggcacggccc
      961 gctggtgttg cgcgccgacc tgtcgccgcg cgcgatccgg cgcatcgcgt ccttcgtcgc
     1021 cagcgccctg atcgacctgc tggccgcgcg ccaagaactg gacgaggcca cccgcaccca
     1081 tctggcgcga aagctgcgtt cgcgccggca gcaagaaacc aagacagccg gcaaaccgcc
     1141 ggcgagtgcc gcagccgaag tcgaggctgc ttgcagggcg ggcaagcttg acgacgcctt
     1201 cgtcgcggcc gcggcggagg cataccagaa ggatacggtg acgcgggcat tgtccgtgct
     1261 ggcgaaaacc ggcgaaacca cggtgcgccg cattctggac tccggctcgg cgaaggcggt
     1321 gacggcgctg tgctggcggg cgggcctcag catgcggatc gccttcaaac tgcagaacac
     1381 gatcatgcgg ctgaaggggc cggacctctt gcctgcgcgc ggcggcatcg atttcccgct
     1441 gagcgaagac gagatgcgct ggcacctggg ctatttcggg atcgtttgag cgcagccgaa
     1501 gctgtgcatc tccaccccat aaagcctgcg gatgaccgcc ggggttatga tgtcttccgg
     1561 catgccgtcg tcggcgatcc gccccgcctc caaggtcgtc acccgggtgg cgatgatggc
     1621 ggcgtgatcg ggctgatgcg tggtcatcag catgccgtag ccctcaccct tcaggccgcg
     1681 caggcgttcc atcagttcca tctgatggcc gtaatcaagg ccggtaaagg gttcgtccat
     1741 caccagcagg cgcgcccctt gcgccagggc ccggccgatc atcaccagtt ggcgctcgcc
     1801 ggcggagagc tgggtatagc ggcggccggc cagcggcgtg atgccgagac tttccatcac
     1861 cgtatccacc gcctctcgat cgctgcggga gatttgaccg aacaggccgg tggaaggcat
     1921 ccggcccatt cccaccacct cgcgcaccag ataggggaag ggcggctgat gcagttgcgg
     1981 tacgtaggcg acccgccgcg ccaaagtccg gcgcgagagg ccggaaagcg gccggccgtc
     2041 aagccggacg ataccgcgtt cgggatgcag gaagccgagc atgatcctga gcagcgtcgt
     2101 ctttccggcg ccgttgcggc ccaacaggca aacgatctcg cgcgaggaca gcgacagcgt
     2161 ggcgcccgcc agcaccgcgc ggctctggcg ggaaaaatgg acggcttcgg cggcgaggac
     2221 ttcaacgctc attccacccc cggctggtgc ggctgagaac caacaggaat atcggaatgc
     2281 cgagcagctc ggtcacaatg ccgatgggga tttcggtgtc ggcaagcgtg cgcgcgagat
     2341 aatcggcgcc aagcaggaac atcgccccga acagagcgga cgacggcatc agccgcgtgt
     2401 tggaggcgcc caaagcgagc cgcacgatat ggggcacgat gagaccgatc cagccgatca
     2461 tgccggcaat cgaaacagtc agggaggaaa tcagcgtcgc cgtggcgatg acggcaaaac
     2521 ggatcacgcc caccggcacg cccagcgagc gcgcttcgtc gtcgcccatg gcgagggcgt
     2581 ccagggcccg gcccagcacg ccgagaagca gcacgccgcc gaacatcggg attgtgagcc
     2641 aggcgatctg gtgaaggttc gactgcgcca gattgcccat cagccaatag acgatgttgg
     2701 gcagcgcatt catcggatcg gcaatgtact tggtgatcga caggagcgaa gcgaacagcg
     2761 cgccgctgac catgccgccg aggaccaaag tcaaggttgc ggcattgccg aacaggcgcg
     2821 ccaccgcgac tccgaacacg accgcgccca gtcccatcac gaaagcgagc gattggacga
     2881 cgtaccagct tacgcccagc acgatgccca aggcggcgcc gaacgaggcc ccgctcaaca
     2941 cgccgagcag tcccggcgat accagcggat tgcggaatac cgcctggaac gccgcgccgg
     3001 aactggccag cgccgatccc accaatacgg cggacaagac gcggggcaag cggatgtcga
     3061 tgatcacatt ggcaagcgcc gcataacggt ccgccgccat gtcgcaaagc ccgagcgagg
     3121 ccaggaagaa acgcgggatt tcgccgatcg cgacgggata tttgccgacg ctgagcgcca
     3181 gcatcatcgc cagcaccagc ccggcggcca atacaagttc ccacggcggg ccggtcgccg
     3241 ccacgatgcg ccgcaaggca tggcggcgga cttgtttcct ctccatctcc gcgagggtcg
     3301 aacagctcat ggtgaaagga gcttttcgac ctgggcgtcg ctcaggctgg tgccgaagaa
     3361 gacatggtgg aagcggcgga tttccgcctt gagatcgatg cgatagacct tggggtgcag
     3421 ttttgtcccc agccattcgg ccccgagcgc ccgcatgaag gaaggcggcc ggtcgaacca
     3481 gttgaaggga taggccggca ccgcaaacac tttgccgtcc ctgaccgccc tcaagcgcgc
     3541 ccagcgcgga tcggatttga tggccgaggc cgtggcggga aagcgcgtga cgatgacgtc
     3601 gggatcgacg gtgagaaggg tttcgagatc gatcggataa tgatcggcca gcagatccgc
     3661 cttggagcag gtgagcgcat tgcgtccgcc cgccaggacg atcacttccg agcggtcggt
     3721 cccttcgcat tgcgatacga gaccgtcgat gctttcggcg taatagacgc gcggtttttc
     3781 cttttccggc acggcggcgg cgtatttttt caaccgggcg acgagaacct ccagtgtctt
     3841 ggccagttcc tcaccgcgcg cctcgcggtg gaacagcttg ccgaggaagc ggaaagtacc
     3901 cggatactgc gacaggcgat caacgtcgat gaacaccacc ggcacattga tccgctccag
     3961 cagcttcacc tgatacatct ctttcggcgt cgtccagccg aaggcaacgt cgatgccgac
     4021 cgccttcagc ttctccatat tgacgtcggc gccgccgccg atattgggca atcccgccat
     4081 acccggcggc aggaattcca gcatctccgg cttgggcggc accggcatgg caaatcccgc
     4141 caccagatcg ggcgcaatcg ccgtgatcag gggaaaggca tgcgtcatcg cataggcgcg
     4201 atggatggtg tccggcacga tgacggggcg gcccgacata tcgacaatct cgcgcgccgg
     4261 cgccggcgca accgcaatcg cgagagccgc cgccaagccc agcgcccagc cgtaacgccg
     4321 cgtgcccgcc atcgcgccct cccctagttc aacagtgaca gtgtgagttt tacagtgcgc
     4381 ggttcgccga cgaaagccgt ggaacccgag gccgtgccgc tgccgtcgat gctgcccggg
     4441 aagagagagg cccaatagcg ctcgtcgaag aggttgttca cggaaaggcg cagcgtgtat
     4501 ttgcccgata tggttccttc cggcagatcg ccggtgtagc gcaggcccag atcgaccgtg
     4561 gcgtagccgt cggccgtcgt cgtgccgtag gtgttggccg tgcgctcgcc cgtgtagtgc
     4621 aggttgaggc tggcggtcac gccggcaaac ggctgcggca ggcgatattc cgtgaacaga
     4681 ttgccctgcc attccggcac gcccaccgcg cgtttgccgt cggtggcctc cgtgtcggtg
     4741 ccggtgatct tggtatcgag ccaggtgaaa ccgccgaaca gagtgatgtc gtcgctcagc
     4801 ttgccctttg ccatgacttc ggcgccgcgg ttccgctgct ggccctggac cttgtaaatc
     4861 ttgtcgggtc cggtccaagc ggtaggccgc tcgatgtgaa acagcgcaaa atcgatttcg
     4921 acgccggaca actcggtctt gacgcccgcc tcccactgcg tgctttcgaa gggatcgagg
     4981 aagtcatagg cgttgagcgc ggtcgacggc gcctggccgg gatcgggatt cacgctggag
     5041 gcgtaattgg cgtagatggt gacgttcttc cgcggcttgt aggaaaggcc gagtgtgtag
     5101 gacgtcgcat cgtcgatatc cgtcacca
//
LOCUS       DASWPU010000569         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_60117,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000569 DASWPU010000000
VERSION     DASWPU010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60117"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..117
                     /locus_tag="VGC27_12005"
     CDS             4..117
                     /locus_tag="VGC27_12005"
                     /inference="COORDINATES: protein motif:HMM:NF023379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3470 domain-containing protein"
                     /protein_id="HEY0283334.1"
                     /translation="MWPNITQKGTPPADAEKYRDEKGKYEKYFSEKPGSGV"
     gene            431..991
                     /locus_tag="VGC27_12010"
     CDS             431..991
                     /locus_tag="VGC27_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY0283335.1"
                     /translation="MTTAKTIVAPVAAKKVPANSNKKHDFKANDHVVYPTHGVGKITR
                     IEEQEVAGTKLELFVITFDKDKMTLRVPTLKAKLVGMRKLSSPDTVATALNTLKGRAR
                     IKRTMWSRRAQEYEAKINSGDLVSIAEVVRDLHRASGQPEQSYSERQLYEAALARMAR
                     EVAAVERTDEQTAVKRVETMLLKKAA"
     gene            complement(1279..2055)
                     /locus_tag="VGC27_12015"
     CDS             complement(1279..2055)
                     /locus_tag="VGC27_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY0283336.1"
                     /translation="MDWRIPAFVAAALTCTGAQASAPPCAGEVEIGDAPVVRVEHNDV
                     LILRDGRALHLEGVRLPHAARDRAPQAVEDQTYDALNALAKNQDVMAHAVWPKQDRYD
                     RVRAQVFTKNGTWLQTELLRRGLARVEIAPDRGECYRELYDAEGVARAARLGLWSLPA
                     YAIRTPETLKADVGTFQIVFGKVLSADKRDGRVFLNFGPDWKTDFTVTISPDDMKTFR
                     RMGVDPLDYQDKLIRVRGIVQWFNGPEIEIGNPKQIELLQ"
     gene            2303..2911
                     /locus_tag="VGC27_12020"
     CDS             2303..2911
                     /locus_tag="VGC27_12020"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY0283337.1"
                     /translation="MRSAILALFALTLAAAASSAQSVDPRQAPTGRYQLEQNHSLVLF
                     AISHLDLTDYYGRFDKLSGTLNFDAAEPEKSAVSITIETASINTPSTKLTGELASAVV
                     FDAGQFPAATFKSTSIQRTGPTTGKITGDLTIKNVTKPVTLDVVFGGGRQNPMGNSYA
                     LGFHATAAIKRSDFGLTSMPWDSFVGDEVKLTIEAMFLHQKE"
     gene            2914..>3258
                     /locus_tag="VGC27_12025"
     CDS             2914..>3258
                     /locus_tag="VGC27_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY0283338.1"
                     /translation="MPAANTQLRYGSVAMTLHWLIAAAVIANLCIGLYMGDLPRGDPD
                     KFTLIALHKSIGLTVLALSVLRVLWRLVNPVPPPPAGLSPWLKTAGRAMQHLLYFLIV
                     AIPLAGWLMVSVG"
BASE COUNT          689 a         1008 c          949 g          612 t
ORIGIN      
        1 aagatttggc ccaacatcac ccagaagggc accccgcccg ccgacgcgga gaagtaccgg
       61 gacgaaaagg gcaagtacga gaagtatttc agtgaaaaac caggttctgg agtgtagaat
      121 taatgatctc gcacttgcga agaagcggta catgggtgac aggatttagc ccgtttcgcc
      181 gccttttgtc tggcaatacc tcattggatt tgaaataagt tttggcaatt caaaggtttg
      241 gtgaaattgc ccttcccttt tctacaacaa tgtgttagct tgtagtcgtt ctgaaataac
      301 tcgaaaggcg ctgccccacc cggattgctg ataggtccgg aggcgccagc attttgttgc
      361 agtgcaaggt gatgcgatgc cttcctctgg acggcgcggt ttcgagtgcg cgaaacggaa
      421 acggaaatag atgacgacgg ctaagacgat agttgctccg gtggctgcca agaaagtccc
      481 ggcgaactcc aacaagaaac atgatttcaa ggccaacgat cacgtcgtgt accccaccca
      541 cggcgtcggc aagatcacgc gtatcgaaga gcaggaagtg gcgggcacga aactcgaatt
      601 gttcgtcatc accttcgaca aggacaagat gaccctgcgg gttccgacct tgaaggccaa
      661 attggtcggc atgcgcaagc tctcctcccc cgatacggtg gcgaccgccc tcaacacgct
      721 gaagggacgc gcgcgcatca agcgcaccat gtggtcccga cgggcgcagg aatacgaagc
      781 gaagatcaac tccggcgatc tggtttcgat cgcggaagtg gtgcgcgacc tgcaccgcgc
      841 cagcggccag ccggaacaat cttattccga gcggcagctc tacgaagcgg cgctggcccg
      901 catggcccgc gaagtcgcgg cggtggaacg cacggacgaa cagaccgcgg tcaagcgcgt
      961 cgaaaccatg ctgctgaaga aggcggcgta agacgcaaac ggaattggca tgagaagagg
     1021 cccggaggaa actccgggcc tttttgtttg gcactcacct ctcctttgcg ggaaaatcga
     1081 tccggcgttt gggtctgagc gacagcgaag acgcacaaag tcgaatctca gatggtagcc
     1141 accctagtgt cgtggtttcg aagttcgcac tatccgctat atcggtctgc aagcgaattt
     1201 cggaatcggg acactagccc ccccacccgg ctcgcattcg ctcgccgacc tccccacgag
     1261 gcggaggtaa tcggcagctc attgcagcag ctcgatctgc ttgggattgc cgatttcgat
     1321 ttccgggccg ttgaaccact gcacgatgcc gcgcacccgt atcagtttgt cctgatagtc
     1381 cagcggatcg acgcccatcc ggcggaaggt cttcatgtcg tcgggcgaaa tcgtgacggt
     1441 gaaatccgtc ttccaatccg gtccgaaatt caaaaagaca cggccgtctc tcttatcggc
     1501 gctgaggacc ttgccgaaca cgatctggaa tgtgccgaca tcggccttca gcgtttcggg
     1561 cgtacggatg gcataggccg gcaaggacca gagccccaga cgagcggcac gcgccacgcc
     1621 ctcggcgtcg tacagctcgc gatagcattc gccgcgatcc ggcgcgatct cgacgcgcgc
     1681 caagccccgg cgcagtagtt cggtttgcag ccaggttccg ttcttggtga agacctgcgc
     1741 gcggacgcgg tcgtagcggt cttgcttggg ccagacggcg tgggccatca cgtcctgatt
     1801 tttcgccaga gcattcaggg cgtcgtaggt ctgatcctcc accgcctgcg gcgcgcgatc
     1861 ccgggctgcg tgaggcaggc ggacgccctc cagatgcagc gcccttccgt ctctcagaat
     1921 cagaacgtcg ttatgttcga cccgaacgac gggagcatcg ccgatttcga cctccccggc
     1981 gcagggcggc gccgacgcct gagcgcctgt gcaggtcaat gccgccgcca caaaggccgg
     2041 aatgcgccaa tccatggatg ccccctcccc gtccccgccg tcattgtgca caagtggagc
     2101 gatttcgcac ctcccgcgca caaaaagacg gcgtcaacaa ttcgcaataa tcgaggaaac
     2161 aaatccgcag gctatggaaa cgcggcacaa aattcgtcaa agtcggcgga tcatgtgtta
     2221 ttgggcaata cactcggcat gctgcgcaag agcgtgacgg tccttgcgta agcttcgccg
     2281 gccaaacaga aagggcaatc ccatgcgatc cgccattctc gcgcttttcg ccctcacgct
     2341 cgcggctgcc gcgtccagcg cccaaagcgt ggaccccaga caggcgccga ccggccgcta
     2401 ccaactggaa cagaaccaca gcctggtgct gttcgcgatc tcgcatctgg atcttaccga
     2461 ttattacggg cgcttcgaca agctttccgg cacgctcaat ttcgatgccg ccgagccgga
     2521 aaaaagcgcg gtctccatca ccatcgagac ggcgagcatc aacacgccga gcacgaagct
     2581 gaccggcgag ctggcaagcg ccgtcgtctt cgacgccggc caattcccgg ccgcgacctt
     2641 caaatcgacc tcgatccaac gtaccggccc caccacgggc aagatcacgg gcgatctcac
     2701 gatcaagaac gtgaccaagc cggtgacgct cgatgttgtc ttcggcggcg gccgccagaa
     2761 tccgatggga aattcctacg ccctcggttt ccacgccacg gccgccatca agcgcagcga
     2821 tttcggcctc accagcatgc cgtgggacag tttcgtcggc gacgaggtga agctgaccat
     2881 cgaagccatg ttcctgcatc agaaggaata gccatgcctg ccgccaacac gcaattgcgc
     2941 tacggcagcg tagccatgac gctgcattgg ctgatcgccg ccgccgtgat cgccaacctt
     3001 tgcatcgggt tgtacatggg cgatctgccc cgcggcgatc cggacaaatt cacgctgatc
     3061 gcgctgcaca agtcgatcgg gctgaccgtg ttggcgctga gcgtgctgcg cgtgctgtgg
     3121 cggctggtca atccggttcc gccgccgcca gcgggattgt cgccctggct gaagaccgca
     3181 gggcgcgcga tgcaacatct tctctatttt ctgatcgtcg cgattcccct ggcgggctgg
     3241 ctgatggtct cggtcggc
//
LOCUS       DASWPU010000570         9675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_102542,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000570 DASWPU010000000
VERSION     DASWPU010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9675
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102542"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..2227
                     /locus_tag="VGC27_12030"
     CDS             101..2227
                     /locus_tag="VGC27_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0283339.1"
                     /translation="MRVGIIGTGIIAKEHAQAIAMLSGSIKLVAAADIAPDRLQSFCA
                     AFGVSRRYSSAAELIADPEVDLVAVATPPSFHEEAVVAALDAGKYVLCEKPLAQSLAS
                     AARIAAAEARHPGHLSVNYQLRYAPQYRRMLWLIENGWIGTVESASVERHGYIPHATI
                     GKGAWWGAWSVAGGGVLMTQMIHELDILLLATGQPRSVNAQMDTRYTSIESEDWIEGK
                     LLFDNGRSARFAASVNSGQMRGGFVVKGSLGTISPGRLTLGDAGREAKALAAVNAALP
                     DTKPASMSLPSRALRKVARKLGAAEKPELSAHALLYRDIAASIAKGAAMPIPASEAMK
                     TLQLCAGAYESAITAKEVALPLDTEAKTYAGVTKEGYASSPRPKPVVIPPVPLLPKTN
                     YVRVGLIGLDTTHVTTFADLLHNPYNGDHIPGAKVVAGFAGGSPDMAASADRVGGFTA
                     ELRDKYGVAILDTPEAVADASDIVFILSCDGRTHPGLFASVAGRSRPVFIDKPLAVSL
                     ADAQQIYELAAKTNTKIFASSAFRWSDGLVAALNSIRASGEKITGCRVRYWGQIQPTQ
                     GRFFWYGIHGADMLMAVMGCGASTVEARTDGTKDYIEIEHKDGRRSTMIGDHYDGTFE
                     VSIDTDKRTIDIPAGGAISARALAAALDVLTPGGYPRLWQASDAGSVSGRPGKTVDPD
                     AAETLDVIALLDAAERSYTGKQKIAL"
     gene            2242..3573
                     /locus_tag="VGC27_12035"
     CDS             2242..3573
                     /locus_tag="VGC27_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0283340.1"
                     /translation="MLRSLRMVVGPLAKKALVAANIEPALIKAIVKKPPVIHGGPRAR
                     RWPWPRRRHFDKRERDAVMRLMNREIRRGGAIVYGGPEEKAYCEAFAEYLGGGFAKAV
                     NSGTNAVYVALRALDLEPGSEVIVPPITDQGGCMPVAMMNCIPIPADSDSGSINTSAE
                     QIAKVITDRTSALLVAHITGHPLDMDPILELAARRKIPVVEDCAQAHGALYKGRMVGT
                     LTEISAFSTMFGKQHSTGAQGGVVFTRDTMLFARARQIADRGKPAGVVCPTGNAVASL
                     NFNQDEISMAIGRVQLEKLPQAVEARRAFVAVVEQGLKGVDGLSLISPPKDSAGSYWF
                     LLVKLDFSKLRCTAAEFAGALELEGIGGVQCGYPFFPSDHPWYRDAIVYGSSGLPWSI
                     VQQKPRHFELPNAHEANRMMVRVDVHEQLGPNEARDLVVALKKLARYFAVK"
     gene            3570..4676
                     /locus_tag="VGC27_12040"
     CDS             3570..4676
                     /locus_tag="VGC27_12040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEY0283341.1"
                     /translation="MTAAVSIITPTKNRLALLCRTMDSVRQQSFQAWEHIVVDDGSDD
                     GTEEEVTRRAAADPRVRYIRRTGAKSGANVCRNIGIRESRGDLIVFLDSDDLLRPKCL
                     ERRVELMGRNADLDFAVFRAGVFSKTPGDLTRLYHNHFHGDDLLRFLSHECIWEITGP
                     IWRKNFLQEIGGFDETLLRMQDLELHVRAICARGIYICLAEVDHDVSWLYDFSRTSVR
                     HMKDGGFIEASETVRKKLRESVLRSGLLTWSRERAIIGLGFENALSWVRLGNLWQALK
                     SWSLCCRNERVPIHLHIGGLLMLAVFRLTVDVTTPHLDKPTLPVRLVNKWSGWVRFRQ
                     EPALVEADQRVGGAESSYGGTNNSKSGKRGTTSP"
     gene            4673..5620
                     /locus_tag="VGC27_12045"
     CDS             4673..5620
                     /locus_tag="VGC27_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283342.1"
                     /translation="MSDEMKSSPPRKQVDCVYIAAFRGDPRLTRICVASVRYFYPDIP
                     LRVLAGSPLLPSLVGELERYWNTRVADLPDAGRDYGSGFVKLEPLFTPPGERFLMLDA
                     DTVLAGPVLDLWREGDAPFLVDEEEQPEAEAKRLYFDWDKLSANGLPTPRPTAVFNTG
                     QWFGTGGLLTRDDFAPLVEWSMPRRLRYPKFFMTGDQGIQNYVFNQKMRDGSIPVQLR
                     KIMFWPVWKMDGMNAAALAARTAPAFVVHWAGLKKRRLRDMVRPDMLDFFERYYYQRV
                     PFGSVLRPFRALTDVLSQWLHDKRVRVRMRLQKIRHGGR"
     gene            5689..6459
                     /locus_tag="VGC27_12050"
     CDS             5689..6459
                     /locus_tag="VGC27_12050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0283343.1"
                     /translation="MSLVKTAKHSLRTVLEAAGYWVRHRSVLPFGIDYQLDIQRMADL
                     MGIPVGTFFDIGAHTGETSLDVLRKFPGVRIFAFEAHPTTFSVLISNVHDARFDGFNI
                     ALSDKNGKAQFYEYGYLATCNSLVGDNQFAVQSKNPSKVLEVECQTLDGFCGAHGIEK
                     IDVLKIDTEGHDLSVLRGAEQTLAARGVSFVYIEFNSMLPRPGTTGGALMPITAILEP
                     LGFHFVASYPECMITTGELLVTSNALFVRPPSRSAPHS"
     gene            6601..7776
                     /locus_tag="VGC27_12055"
     CDS             6601..7776
                     /locus_tag="VGC27_12055"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0283344.1"
                     /translation="MGMLRLFLAISVLVAHTGPIFGLNLMFSDVAVKLFFIISGFYMS
                     LVLSGKYEGGSLRWTFYSNRFLRLYPAFLLVTLTSLGVFLAAEFMPNAPERSIYVSEI
                     VRRFSGAHGQLGGFSLVALMIPNIMIVGSDIAFLFHHSVNTGWEFTFGILAHYPGAVR
                     GGSYLLNPPAWSIGIELWFYLLVPTLSRAKTPIIILLAAASLALRLWMDWKHPWITYF
                     FFPAALCFFLYGMLAHRFWASSLFKSVAIPSRVWAIAGCGLALLMLREFIPGYRNHPW
                     MIYTVAVFSLPFVFQAFKNIRWDRWVGNMSYPVYLFHTVIIAAVYDFLGSHSTPLIIG
                     LTLAVSIVVNFFVEEPLERFRQRRASKQKSAGISGSDTISPAGSTLGVEESRQVPVP"
     gene            7783..8703
                     /locus_tag="VGC27_12060"
     CDS             7783..8703
                     /locus_tag="VGC27_12060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0283345.1"
                     /translation="MSAKFLSVIICTHNPRPDYFGRTLDALRGQSLPREQWELVIVDN
                     RSEPPVAGRIDFGWHPRARVIREEKLGLTPARLCGIREAAGDLFVFVDDDNVLDADYL
                     ETALRIAEQRSFLGSWSGQSRPEFEETPPEWTRRYWGNLVIREFDDDKWSNLPRLPET
                     MPCGAGLCVRREVALHYLHLNESGKRGIQFDRTGTSLLSGGDNDLAACACDLGLGVGL
                     LSALKLTHLIPPVRLTEDYLARLAEGISFSSVLLDHEWGIRNEPRGLAGRVMDFLRTI
                     RLRQPHRRIVKAANRGRNRAIELLASKRAG"
     gene            8747..>9675
                     /locus_tag="VGC27_12065"
     CDS             8747..>9675
                     /locus_tag="VGC27_12065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0283346.1"
                     /translation="MRAPRVLHIVESLDRGAVENWLVRMLRHARARGTDIDWDFYCIL
                     PQPGTLDAEAASLGARVLFSPVPIGEKGAFVRALRAELRHGKYDVLHCHHDIISAVYL
                     LASTGLPINRRIVHVHNADENVLTPSRLKQKLFREPLRRVCLGFADRIVGISNITLDT
                     FLAGRARRPGRDVVHYYGIDPAAFRAASGDRSGFRRSLGFGEETRILLFAGRMVPEKN
                     PLFALDVLAEMRRLDPAVAGVFVGSGSLDGALAARAAELGLSANFRALGWRGDVPDIM
                     CCCDWFILPRPEQPMEGFGIAVVEAQLGGLRMLL"
BASE COUNT         1803 a         2852 c         2976 g         2044 t
ORIGIN      
        1 aaaccgtgac gctagaccac atcctcgcag tgatcgaatt cactctatac ggcgtggcaa
       61 tctcgattgt gtcactcgaa catctgaaag tcttcaaatg atgagagttg gaataatcgg
      121 tactggcatc atcgccaaag agcacgctca ggcgatcgcc atgctctccg gcagcattaa
      181 gctggtcgcc gccgccgaca tcgcccccga ccgtcttcag agtttttgtg cggcgttcgg
      241 cgtgtcgcgc cgatattcga gcgcggccga actgatcgcg gatcccgaag tggaccttgt
      301 cgccgtcgcc acgccgccgt cgttccacga ggaggccgtg gtcgccgcgc tggacgccgg
      361 aaaatacgtc ctgtgcgaaa agccgctggc ccagtcgctg gcgagcgccg cgcgtatcgc
      421 cgccgccgag gcgcgccatc ccgggcatct ctcggttaac tatcagcttc gctatgcgcc
      481 gcaataccgg cggatgctgt ggttgatcga gaacggttgg atcggcacgg tcgaaagcgc
      541 cagcgtcgag cgtcacggct acatcccgca cgccaccatc ggcaagggcg cctggtgggg
      601 cgcatggagc gtcgcgggcg gcggcgtgct gatgacccag atgatccatg aactggatat
      661 tcttctgctg gcgacggggc agccgcgctc ggtcaacgcc cagatggaca cccgctacac
      721 cagcatcgaa tctgaggatt ggatcgaggg caaacttctg ttcgacaacg gccgcagcgc
      781 gcgtttcgcg gcatccgtca attcgggcca gatgcgggga gggttcgttg tcaagggaag
      841 ccttggaacc atttcgccgg gcaggttgac gcttggcgat gccggtcgtg aggcaaaagc
      901 gctcgccgcc gtcaacgccg cgctgccgga caccaagccg gcctcgatgt ctctgcccag
      961 ccgcgcgttg cgcaaggtcg cgcgcaagct tggcgccgcc gagaagcccg agttgagcgc
     1021 tcacgcgctg ctctaccgcg atattgccgc cagcatcgca aaaggggcgg cgatgccgat
     1081 cccggccagc gaggcgatga agacgctgca gctctgcgcc ggagcctatg aatccgccat
     1141 cacggcgaag gaggttgcgc tgccgctcga taccgaggcc aagacgtatg ccggtgtcac
     1201 gaaggaaggc tatgcatcca gcccgcggcc gaagcccgtc gtgattccgc cggttccgct
     1261 cttgccgaag accaattatg tgcgcgtcgg cctgatcgga ctcgacacca cccatgtgac
     1321 gaccttcgcc gatcttctgc acaaccctta caacggcgac cacatccccg gcgcaaaagt
     1381 cgtcgccggc tttgcgggcg gatcgccgga catggccgcg agcgcagatc gcgtcggcgg
     1441 cttcacggcg gaactccgcg acaagtacgg cgtagcgatc ctggatacgc cggaagccgt
     1501 ggcggacgcc tccgacatcg tcttcatcct ttcgtgcgac gggcggaccc atccggggct
     1561 gttcgcgtcc gttgccggcc gcagcaggcc ggtcttcatc gacaagccgc tggccgtgtc
     1621 gttggcggat gcacaacaga tttatgagct tgccgcgaag acgaacacga agatcttcgc
     1681 gagttcggcg tttcgttggt cggacgggct ggttgcggcg ctcaattcga tccgcgcctc
     1741 gggcgagaag atcacgggtt gccgggtccg ctattggggc cagatccagc cgacccaggg
     1801 acggttcttc tggtacggca ttcacggcgc cgacatgttg atggccgtga tgggctgcgg
     1861 cgcttccacg gtggaggcca gaacggatgg cacaaaagac tacatcgaga tcgaacacaa
     1921 ggacggccgg cgctcgacga tgatcggcga tcactacgac ggcacgttcg aagtcagcat
     1981 cgacaccgac aagcgcacca tcgatatccc cgccggcggc gcgatttcgg cccgcgcgtt
     2041 ggcggcagcg ctcgacgtgc tgacgcccgg cgggtatccg aggctctggc aggcttcgga
     2101 cgccggttcg gtctctgggc gtcccggcaa aaccgtggac cccgacgcgg ccgagacgct
     2161 cgacgtgatt gccctgctcg acgccgcaga gcgcagttac accggtaaac aaaagatagc
     2221 gctctgaacg cggagacgaa gatgcttcga tccctgagaa tggtggttgg cccgctggcc
     2281 aagaaagccc tggtggcagc caatatcgag ccggccctga tcaaagccat cgtcaagaaa
     2341 ccgccggtga tacatggcgg cccgcgcgcc cgccgctggc cctggccccg gcggcgtcac
     2401 ttcgacaagc gcgaacgcga cgcggtcatg cgcctgatga atcgcgagat acgtcgcggg
     2461 ggggccatcg tctatggcgg gccggaggaa aaggcgtatt gcgaggcgtt cgccgaatat
     2521 ctcggcggcg gcttcgccaa ggcggtcaat tcgggcacca atgccgtgta cgtcgcgctg
     2581 cgcgcgctgg atctggagcc cggcagcgaa gtgatcgtac cgcccatcac cgaccaaggc
     2641 ggatgcatgc ccgtggcgat gatgaactgc attcccatcc ccgccgattc cgattccggt
     2701 tccatcaata catccgctga gcagatcgcc aaagtcatca ccgatcgcac gtcggcgctt
     2761 ctcgtcgcgc acatcacggg ccatccgttg gatatggacc cgatcctcga actggcggcc
     2821 cggcgcaaaa tcccggtggt ggaagactgc gcccaggccc acggtgccct ctacaaagga
     2881 cgcatggtcg gcacgcttac cgagatttcc gccttttcga cgatgttcgg aaagcagcac
     2941 tccaccggcg cgcaaggcgg agtcgtgttc acgcgcgaca ccatgctttt tgccagggcg
     3001 cggcagatcg ccgaccgcgg caagcccgcc ggcgttgtgt gcccgaccgg caacgcggtg
     3061 gcgtcgctga acttcaatca ggacgagatc agcatggcca tcggccgcgt ccagcttgag
     3121 aagctgcccc aggcggtcga agcgcgaagg gcttttgtcg ccgtcgtcga acagggcttg
     3181 aagggcgttg acggcctgtc gctcatctcc ccgccgaagg actcggccgg ttcgtactgg
     3241 ttcctgctcg tgaaactcga tttttcaaaa ttgcgttgca cggcggcaga gttcgcaggc
     3301 gcactcgaac ttgaaggaat cggcggcgtg cagtgcggct atccgttctt cccgtcggac
     3361 catccctggt atcgcgatgc gatcgtctac ggctcgagcg gccttccttg gtcgatcgtg
     3421 cagcagaaac cgcgacactt cgaattgccg aacgcccacg aggccaaccg gatgatggtt
     3481 cgcgtcgatg ttcatgaaca gcttgggccg aacgaagcta gagatcttgt tgtcgccttg
     3541 aagaagctcg ctcgctattt cgcggtcaaa tgacggcagc cgtttcaatc attacgccga
     3601 ccaagaaccg ccttgcgttg ctgtgccgaa cgatggattc cgtccgccaa cagagcttcc
     3661 aggcatggga acacatcgtc gtcgatgacg ggtcggacga cggcaccgag gaagaggtaa
     3721 cgcggcgggc cgccgccgat ccgcgcgtcc gctatattcg ccgtaccggc gcgaagtccg
     3781 gcgcgaatgt gtgccgcaat atcggtatcc gcgaatcccg gggcgatctg atcgtcttcc
     3841 tggattcgga tgatctgctc aggccgaaat gccttgagcg ccgcgtggaa ctcatggggc
     3901 gaaacgcgga tctggatttc gccgtgttcc gggccggcgt cttctcgaag accccgggcg
     3961 atttgacccg tctgtaccac aatcatttcc acggcgacga tttgcttcgc ttcctttctc
     4021 acgaatgcat atgggagata accggcccca tctggaggaa gaacttcctc caagaaattg
     4081 gcgggttcga cgaaaccctg ctcagaatgc aggacttgga gttgcatgtg cgtgcgatct
     4141 gcgcccgcgg tatatacatt tgtttggcgg aggtggatca cgacgttagc tggctctacg
     4201 atttcagccg aaccagcgtc cgccacatga aggatggcgg ttttatagaa gcgtccgaga
     4261 ccgtccgcaa aaagttgcgg gagtccgttc tccgtagcgg tcttctgacc tggtcgcgcg
     4321 aacgcgccat tatcggactg ggttttgaaa acgctctttc atgggtgcgg ttgggtaacc
     4381 tttggcaggc cttgaaaagt tggagtctat gctgccgcaa cgagagggtt cccatccatc
     4441 tgcatattgg gggcctgctg atgcttgcgg tgtttcgact taccgttgat gtgacgacgc
     4501 cgcaccttga taagccgacg ctgccggttc ggctcgtcaa caagtggagt ggctgggtcc
     4561 gcttcagaca ggaaccggct ctagtagagg cggatcagcg cgtgggcggc gctgaatcgt
     4621 catatggtgg cacaaacaac tccaagagcg ggaaaagggg gacaacatca ccttgagcga
     4681 tgaaatgaaa agctcgccgc cacgaaagca ggtagactgc gtctatatcg cggcattcag
     4741 aggcgatccc cgcctgacgc gtatttgtgt ggccagcgtc cgttatttct atcctgatat
     4801 tccccttcgc gtgctggctg gaagtcccct gctcccaagc ctcgtcggcg aattggaacg
     4861 ctattggaac acacgggtgg ctgatcttcc cgacgcggga cgtgattacg gctcgggttt
     4921 tgtcaagctg gaaccgctct tcactccgcc gggcgagcgc tttttgatgc ttgacgccga
     4981 cacggtgctg gccggaccgg tgctggatct ttggcgggaa ggcgacgcgc cgtttctcgt
     5041 ggacgaggaa gagcaaccgg aggccgaagc caagcggctc tatttcgact gggacaagct
     5101 ctctgcgaac ggacttccta cacctcggcc gacagcggta ttcaacaccg ggcaatggtt
     5161 cggcaccggc ggccttctga cacgagacga cttcgcgccg ctggtcgagt ggagcatgcc
     5221 gcgcagactg cggtatccga agttcttcat gaccggcgac cagggaattc aaaattacgt
     5281 cttcaatcag aaaatgcgag atgggtccat tcccgttcag ctgcgcaaga tcatgttctg
     5341 gccggtgtgg aagatggacg gcatgaacgc cgccgcgctc gcggcacgaa cggcgccggc
     5401 gttcgtcgtc cactgggccg ggctgaagaa gcgccggctg cgcgacatgg tgcgcccgga
     5461 catgctcgat ttcttcgaaa ggtactacta tcagcgggtt cccttcggtt ccgtattgcg
     5521 gccattccgg gccttgacgg atgtcctgtc gcagtggctg cacgacaagc gcgtcagagt
     5581 gcgaatgaga ttgcagaaaa ttcggcacgg tgggcgctag agccgctact gagacgggaa
     5641 ttccgtgaag acatttgagt cgccaaccac tcgttatccg aggcgcgcat gtctctagtg
     5701 aaaacagcga agcattccct gcgcacggtg ctggaagcgg ccggctattg ggtccggcac
     5761 cgctcggtat tgcccttcgg tatcgactac caactcgaca tccagcgaat ggccgatctg
     5821 atgggaattc ccgtcggcac cttcttcgac attggcgccc acacgggtga aacgtcgctg
     5881 gacgtgctgc gcaaattccc aggagtaaga atattcgcgt ttgaagccca tccaaccacg
     5941 ttctccgtgt tgatatcgaa cgtccatgat gcgcggttcg acgggttcaa catcgcgctc
     6001 agcgataaga atggaaaggc gcaattctac gaatatgggt accttgcgac ctgcaacagc
     6061 ttggttgggg ataatcaatt tgcggtgcaa tccaaaaatc catccaaggt actcgaggtg
     6121 gagtgccaga ccttggacgg attttgcggc gcgcacggga ttgagaagat cgatgtgttg
     6181 aagatcgata ccgagggcca cgatctttcc gtgttgcgag gggccgagca aactctcgcg
     6241 gcgcggggcg tcagctttgt ctatattgag tttaatagta tgcttccgag gcccggcaca
     6301 acgggcggcg cgctaatgcc cattactgcc attctcgagc ccctgggatt tcatttcgtc
     6361 gcaagctatc cggaatgcat gatcacgacg ggtgaattgt tggtaacgtc caacgccctg
     6421 tttgttcgcc cgcccagccg atcggcaccg cactcttaat ggccataccg gcgctctccg
     6481 tcatcatctg cacgcacaat cctcggccgg attttacctc gaacgagagc cacgtaattc
     6541 gatgaatctt ccaaagattc gaaagtgacc tcattttcca ggtcgggacg gcgctgaaca
     6601 atgggcatgt tgcggctctt cttggccatt tcagtcctgg tggcgcatac aggccccatc
     6661 tttgggctga atctgatgtt ttccgatgtg gcggtgaagc ttttctttat tatctcaggc
     6721 ttctacatgt cgctggttct ttcggggaaa tacgagggcg ggagcctgcg ttggacattc
     6781 tattccaacc ggttcctgcg cctgtatccc gcttttttgt tggtgactct gacgagcctc
     6841 ggagtgtttc tcgcggcaga atttatgccc aacgccccgg aaaggagcat atatgtcagt
     6901 gaaatagttc gaagattttc aggggcacac ggccagcttg gcggcttttc gttggtcgcg
     6961 ttgatgatcc cgaatatcat gatcgttggc tccgatatcg cgtttctgtt ccatcacagc
     7021 gtcaatactg gctgggagtt caccttcggg attctggcgc actatcccgg cgcagttcgt
     7081 ggcggctcct atcttctcaa tcccccggct tggtctattg gcatcgaatt atggttttat
     7141 ctacttgtgc cgacgctctc gcgtgccaag acgccgatca ttatcctatt ggcggcggcg
     7201 tctttagctc tgcgtttgtg gatggattgg aagcatccat ggattacata tttcttcttt
     7261 ccggcagcac tctgcttttt cctatacggc atgttggcgc atcgtttctg ggcgtcgtcg
     7321 ttgttcaaaa gcgtggcaat accatcgaga gtctgggcaa tagcaggctg tggtctcgca
     7381 cttctgatgt tgcgtgagtt cattccgggt tatcgcaacc atccgtggat gatttacacg
     7441 gtcgcggtat tttccctgcc gttcgtgttc caggcgttca agaacatcag atgggaccgc
     7501 tgggtcggta atatgtccta tccagtgtat ctgtttcaca cagtgatcat cgcggccgtc
     7561 tatgattttc tgggatcaca ctccacgcct ctaattattg gcttgaccct cgcggtgtcc
     7621 attgtcgtca atttcttcgt tgaagagccg ttggagcgct tcaggcagcg ccgggcgagt
     7681 aagcagaaga gtgcgggtat ctcgggttcg gacaccattt ctcctgcggg ctccacactg
     7741 ggcgttgaag aatcccgtca ggtaccggtc ccgtagacat caatgagcgc gaagtttctc
     7801 tccgtcatca tctgcacgca caatcctcgg ccggattatt tcggccggac gctcgatgct
     7861 ttgcgaggcc aatcgctccc gcgcgaacaa tgggagcttg tcattgtaga caatcgcagc
     7921 gagccgccgg ttgcggggcg gatcgatttc ggttggcatc cccgtgcaag ggtcatccgc
     7981 gaggagaagc tgggcctgac gcccgcgcgc ctgtgcggaa tccgcgaggc ggcgggcgat
     8041 ttgttcgttt tcgtggacga cgacaatgtg ctcgacgcgg actatctgga aaccgctttg
     8101 cgcatcgcgg aacagcgctc cttcctgggt tcgtggagcg gacaaagccg cccggaattc
     8161 gaggagacgc cgccggaatg gacgcgacgc tattggggca atctcgtcat ccgcgaattt
     8221 gacgacgaca aatggtcgaa cctgccgcgt ctgcccgaaa ccatgccgtg cggcgccggg
     8281 ctctgcgtgc ggcgtgaggt ggcgctccac tacctgcatt tgaacgaatc cgggaagcgg
     8341 gggattcagt tcgaccgtac cgggacgtcc cttctgtcgg gcggcgacaa cgacttggcg
     8401 gcctgtgcct gcgatttggg cttgggcgtc gggcttttgt ccgcgcttaa gctcactcat
     8461 ttgataccgc cggtgcgatt gaccgaggat tatctggcgc ggttggcgga gggcatttcc
     8521 ttttcgtccg tcctgctgga tcacgaatgg ggcatccgga acgagccgag gggcttggcg
     8581 ggccgcgtga tggatttcct gcgcactatt cgattgcgcc agcctcatcg ccgcatcgtc
     8641 aaggcggcca atcgcggacg aaaccgcgcg atagaactgc tcgcctcgaa acgggccggt
     8701 taagtggacg aatgacaact cgtcagaaac gatcgcagat cgcccgatga gggcgccgcg
     8761 cgttctgcac atcgtcgaaa gcctcgaccg tggggcggtg gagaactggc ttgtgcgcat
     8821 gctgcggcat gcgcgcgcac gcggtaccga catcgattgg gatttctatt gcatcttgcc
     8881 gcaaccggga actctggatg cggaggcagc ttcgctcggc gcccgcgttc ttttctcgcc
     8941 ggttcccatc ggagagaaag gggcgttcgt gcgcgcgttg cgggcggagt tgcggcacgg
     9001 gaaatacgac gtgttgcatt gccatcatga catcatcagc gccgtttacc tgctggcttc
     9061 gacgggactt ccaatcaacc ggcgcatcgt tcacgttcac aacgccgatg aaaatgtcct
     9121 tacgcccagc cgtctcaagc agaagctttt tcgcgagccg ttgcgccgcg tctgcctggg
     9181 ttttgcggat cgcatcgtcg gaatatccaa tatcaccttg gacacttttc ttgccgggcg
     9241 ggcgcggcgg ccggggcgcg acgtcgttca ctattatgga atcgacccgg cggcctttcg
     9301 cgcggcaagt ggagaccgct caggcttccg ccgctcgctc ggctttggcg aagagacgcg
     9361 gatactccta ttcgccggcc gcatggtgcc ggaaaagaac ccgctctttg cgctcgatgt
     9421 cctggcggaa atgcgtcgtc tcgatcccgc cgtcgccggt gttttcgtcg gctccggttc
     9481 gctggacggg gcgcttgccg cccgcgcggc agagttgggc ctgagcgcga attttcgggc
     9541 gctgggctgg cggggcgatg tcccggacat catgtgctgt tgcgactggt tcatcctgcc
     9601 gcggcccgaa cagcccatgg agggcttcgg aattgccgtc gtggaagctc agctcggcgg
     9661 actgaggatg ttgct
//
LOCUS       DASWPU010000571         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_102559,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000571 DASWPU010000000
VERSION     DASWPU010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102559"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VGC27_12070"
     CDS             complement(<1..738)
                     /locus_tag="VGC27_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0283347.1"
                     /translation="MRVKQPRRILAFDIGATGLKAAVVDQKGKLLSEHLRVPTPHPCP
                     PALLIKTFVGMVKPFKNFDCLSIGFPGMVRGSRVLTAPNLGSELWAGYDLAGVLSKKL
                     GKPARIVNDADMQGLAVVKGKGLEFVITLGTGLGTALFRDGELMPHMEIAHMPACDGK
                     DFDGYIGDKVRKKIGLQKWNKRMERVLPYFHTLLHYDRLYIGGGNAGRLTIKLPRKST
                     IVPNIAGLKGGAALWRSDLHAIHGTASN"
     gene            complement(756..1475)
                     /gene="pgl"
                     /locus_tag="VGC27_12075"
     CDS             complement(756..1475)
                     /gene="pgl"
                     /locus_tag="VGC27_12075"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY0283348.1"
                     /translation="MTAPRTEIFDDAEQFAARAAAWIAGLIAAKNGRFRIALTGGETP
                     RALYRELGARFRASVDWTKVEFFWGDERFVPPESPASNYRMARETLLAQISAPEGNIH
                     PIPTGGAPDEAARSYEATLKKAYGAASLDPARPLFDLVLLGLGDDGHICSLLPGSPAL
                     EERRRWAVAVTQGRPEARITLTYPPVQASRVTAFLVAGENKAKAVRSVRAGDLALPGA
                     RLQPEGELVWLLDRAAAGLIR"
     gene            complement(1472..2464)
                     /gene="gnd"
                     /locus_tag="VGC27_12080"
     CDS             complement(1472..2464)
                     /gene="gnd"
                     /locus_tag="VGC27_12080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY0283349.1"
                     /translation="MEIGIVGLGRMGANIARRLMRNGHSVVAFDVNPDAVAALTREGA
                     KGANSLPALIAALKPPRAVWVMLPAGKVTEGAVAELAGHLSAGDTVIDGGNTFYKDDV
                     RRAAELRLKDIHYVDVGTSGGIWGLERGYCMMIGGETSIVDRLDPIFATLAPGAGTIP
                     KTANREGRDPRVENGYVHTGPVGSGHFVKMVHNGIEYGLMQAYAEGFDILRHRSAEAL
                     PGDHRFKLDLADVAEVWRRGSVIASWLLGLTAMALAEHGDLENFSGFVEDSGEGRWTV
                     EAAIEEAVPANVLSAALYARFHSREERSFADRLLSAMRSKFGGHVEGRPPEPDK"
     gene            complement(2476..>3375)
                     /locus_tag="VGC27_12085"
     CDS             complement(2476..>3375)
                     /locus_tag="VGC27_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283350.1"
                     /translation="VIASKGLASFGAWMEQLIAESTGKKGKGLIPVDGEPLGTADSYG
                     SDRVFVALRLAGEDIPEQLLKALAAKGHPVVTIDVDTPSRLPRLFFLWEIATAVAGAV
                     LGINPFDQPDVEAAKIKTRALMEAGGAKQNEKPLVSSGGVSLYADAANAKALAGAGTL
                     EASLKAHFARVKAGDYTALLAFIERNAANTAELDAIRALVRDKTGAASCLGFGPRFLH
                     STGQAYKGGPDTGVFLQIACDHARDIEVPGRGYSFGAVIDAQAAGDLAVLAERGRRAI
                     RIHLHDVPEGLRTLRSAIETALR"
BASE COUNT          538 a         1140 c         1088 g          609 t
ORIGIN      
        1 gttcgaggcg gtgccgtgaa tggcgtgaag gtccgaccgc cacagcgccg cgccgccttt
       61 gaggccggcg atgttgggaa cgatcgtgct cttgcgcggc agcttgatgg tgagccttcc
      121 ggcgttgccg ccgccgatat agagacggtc gtaatggagc agcgtatgaa aatacggcaa
      181 tactcgctcc atgcgtttgt tccacttttg caggccgatc ttcttgcgca ccttgtcgcc
      241 gatatagccg tcgaagtcct tgccgtcgca cgctggcatg tgcgcgattt ccatgtgcgg
      301 catcaattcg ccgtcgcgga acaaggcggt gcccaggccc gttcccagcg tgatcacgaa
      361 ctccagccct ttgcccttga ccacggccag gccttgcatg tcggcgtcgt tgacgatccg
      421 cgccggcttg ccgagctttt tcgacaagac gccggcgaga tcgtagccgg cccacagctc
      481 ggacccgaga ttgggcgcgg tgagcacgcg cgagccgcgc accatgccgg gaaagccgat
      541 ggagagacag tcgaaattct tgaacggctt caccatcccg acgaacgtct tgatcagaag
      601 cgcgggcggg cagggatgcg gcgtcggcac gcgcaggtgc tcgctcaaga gcttgccctt
      661 ctgatcgacg acggcggcct tgagcccggt tgccccgatg tcgaaggcca ggattcgtct
      721 gggttgcttt acgcgcatct caccccctga ttttattatc gtatcaatcc ggcggcggca
      781 cggtccaaaa gccagaccag ttcgccttcc ggctgcaaac gcgccccggg caacgccaga
      841 tcgccggcgc gaacggagcg gacagccttc gctttgttct ctcccgcgac cagaaacgcc
      901 gtcacgcggc tggcctgaac cggcgggtag gtaagcgtga ttctcgcctc cggcctaccc
      961 tgggtgacgg caacggccca gcgcctccgt tcctccaggg ccggcgaacc cggcagcagc
     1021 gaacagatgt gcccgtcgtc gcccagcccc agcagaacca gatcgaacag cggcctggcg
     1081 gggtcgagcg acgccgcgcc ataggccttt ttgagcgtcg cttcgtagct tcgagcggcc
     1141 tcgtccggag cgccgccggt cgggatcggg tggatgttcc cttcgggcgc ggaaatctgc
     1201 gccagcagcg tctcgcgcgc catgcggtag ttgctggccg ggctttcggg cggcacgaag
     1261 cgctcgtcgc cccagaagaa ctccaccttc gtccaatcca cgctggcgcg gaagcgggcc
     1321 ccgagttcgc ggtagagcgc gcgcggcgtt tctccgccgg tcagcgcgat gcggaagcgc
     1381 ccgttcttgg cggcgatcag gccggcgatc caggcggccg cccgcgccgc gaattgctcc
     1441 gcatcgtcga atatttcggt gcgcggcgcg gtcacttgtc cggttcgggc ggacggcctt
     1501 caacatggcc gccgaatttg ctgcgcatcg cggacaacag ccggtcggcg aagcttcgtt
     1561 cttcgcgcga gtggaagcgc gcgtaaagcg ccgcggacag cacgtttgcg ggcaccgcct
     1621 cttctatcgc cgcctcgacg gtccagcgtc cttcgccgga atcctcgacg aagccggaga
     1681 agttttcgag gtcgccatgt tcggccagcg ccatcgcggt gaggcccagg agccaggatg
     1741 cgatcacgct gccccggcgc catacttccg cgacgtcggc gagatcgagc ttgaagcgat
     1801 ggtccccggg cagagcctcg gccgagcggt ggcgcaggat gtcgaagcct tcggcatagg
     1861 cctgcatcaa gccgtactcg atgccgttat ggaccatttt cacgaaatgg cccgaaccca
     1921 ccggaccggt atgcacataa ccgttctcga cgcgcggatc gcgcccctcg cggtttgccg
     1981 tcttcgggat cgtgcccgcc ccaggagcca gcgtcgcaaa gatcgggtcc aggcgatcga
     2041 cgatgctcgt ttcgccgccg atcatcatgc aatagccgcg ttccagcccc cagatccctc
     2101 ccgaagtgcc gacatcgaca taatggatgt ctttgagccg cagctcggcg gcgcgccgca
     2161 catcgtcttt gtagaacgta ttgccgccgt cgatcaccgt gtccccggcg ctcagatggc
     2221 ccgccagttc cgcgaccgcg ccctcggtca cttttcccgc cggcagcatg acccagacgg
     2281 cgcgcggcgg cttgagcgcg gcgataaggg cgggcaagga atttgctccc ttggcgccct
     2341 cgcgcgtcag cgccgccacc gcgtccggat tgacatcgaa cgcgacgacg ctgtgcccgt
     2401 ttcgcatcag ccgccgggcg atattcgcgc ccatgcggcc cagaccgacg atgccgattt
     2461 ccatgccgtg gtccttcagc gcaaggctgt ttcgatggcg ctgcgaagcg tgcgcaagcc
     2521 ctccggaacg tcatgcaggt ggatgcgaat ggcgcgcctg ccgcgttcgg ccagcaccgc
     2581 gagatcgcct gccgcttgtg cgtcgatgac ggcgccgaag ctgtagccgc gccccggcac
     2641 ttcgatgtcg cgggcatgat cgcaggcgat ctgcaggaag acgccggtgt cgggcccgcc
     2701 cttataggct tgcccggtcg aatgcaggaa gcgcggcccg aagcccaggc acgaagccgc
     2761 cccggtcttg tcgcggacga gcgcgcggat ggcgtcgagt tccgccgtgt tcgccgcatt
     2821 gcgctcgatg aacgccagca gggcggtgta gtcgcctgcc ttcacgcggg cgaaatgggc
     2881 cttcagagag gcttccagcg tcccggcccc ggccagcgcc ttggcgttcg ccgcgtcggc
     2941 ataaagcgaa acgccgccgc tcgaaaccag cggtttttcg ttttgtttgg cgccgccggc
     3001 ttccatcagg gcgcgggtct tgatttttgc cgcttccacg tcgggctggt cgaaaggatt
     3061 gatgccgaga accgcgccgg caaccgccgt tgcgatctcc cacaggaaga acagccgcgg
     3121 caggcggctc ggcgtatcga cgtcgatcgt gacgaccgga tgccctttgg cggccagcgc
     3181 cttcagcagt tgctcgggta tgtcttcgcc ggcgaggcgc agcgccacga acaccctgtc
     3241 ggagccatag ctgtcggctg tgccgagcgg ttcgccgtcg accgggatca ggcctttgcc
     3301 tttcttgccg gtcgattccg cgatcagctg ctccatccag gcgccgaagc tcgccagccc
     3361 tttcgaagcg atcac
//
LOCUS       DASWPU010000572         3444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_4735, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000572 DASWPU010000000
VERSION     DASWPU010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4735"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1256
                     /locus_tag="VGC27_12090"
     CDS             60..1256
                     /locus_tag="VGC27_12090"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY0283351.1"
                     /translation="MTTFRTIDDLDVKSKRVLVRADLNVPMKDGIVTDTSRIDRQAPT
                     VTQLAERGARVIVCSHFDRPKGKVVPSMSLAPVSGPLAKAVGRPVAFASDCVGKLAEE
                     AVAKLKDGDVLLLENTRFHAGEEKNDPELAKQLAALADLYVNDAFSSAHRAHASTEGV
                     AHLLPSAAGRSMEAELTHLHKALNDPERPLMAVIGGAKVSTKIALLENLVKRVETLVI
                     GGAMANTFLAAQGKNIGKSLHEHDHLETARRVIAQSVESGVALLLPTDAVVAKEFKAG
                     AASRTVPIDQVGDDEMILDIGPASVAEFEKFLHNTRTVVWNGPLGAFETSPFEKGTVA
                     AAKLVAQRTKAGQLLSVAGGGDTVAALAMAGVKDDFSYVSTAGGAFLEWMEGLVLPGV
                     EALMVK"
     gene            1380..2297
                     /locus_tag="VGC27_12095"
     CDS             1380..2297
                     /locus_tag="VGC27_12095"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY0283352.1"
                     /translation="MSDAVQKILSWYESDNPGVKTNLARMLYHGRLGGTGKLVILPVD
                     QGYEHGPDRCFVPNPPAYDPHYHFQLAIDAGLSAFASPLGMLEAGAATFAGRIPLILK
                     VNSANLLYPKDGPKTQAVTASVHDALRLGCTAVGLTIYPGSLRSYDMFEVARDFVAEA
                     KDVGLPTVIWSYPRGEDISKDGETAIDVDAYATQIAALLGAHIIKVKLPTSFVEAKDI
                     AELYKKGNVKLDTLPDRVALIKKAALGGRRIVIFSGGGKKADEEILEEARAIKAGGGD
                     GSIMGRNAFQRSKADAMALLDKVIEIYKD"
     gene            2388..3008
                     /gene="thiE"
                     /locus_tag="VGC27_12100"
     CDS             2388..3008
                     /gene="thiE"
                     /locus_tag="VGC27_12100"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0283353.1"
                     /translation="MTTRCRLYLITPPKLEPRAFAETLKRTLGAGDVASLQLRLKDVS
                     DEEIVGAADILMFVAQQAGVAFILNDRPDLAAKIGCDGVHVGQEDASYAEARAALGSD
                     KIVGVTCHASRHLAIEAADAGADYVAFGAFFPTATKEAKAHADLDILQWWSELMVVPC
                     VAIGGITVANAKPLVDAGADFLAVSSGVWDHPDGPAAAVKAFNVLF"
     gene            3147..>3444
                     /locus_tag="VGC27_12105"
     CDS             3147..>3444
                     /locus_tag="VGC27_12105"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY0283354.1"
                     /translation="MTKTLRPGVVTGPDYLQLLAACKEGGYALPAVNIVGTSGINAVL
                     ETAAKAKSDVIVQMSNGGARFYAGEGAPDAHRARVLGATSAAQHVHLLAAEYGVC"
BASE COUNT          637 a         1133 c         1099 g          575 t
ORIGIN      
        1 acatgcacgc tttcgtatct cgcaccgcca tcccagggtg gaaacacagg acaagcgcca
       61 tgaccacgtt ccgcaccatc gacgacctag acgtgaaatc gaagcgcgtg ctcgtgcgcg
      121 cggacctcaa cgtgccgatg aaggacggga tcgtcaccga cacctcccgc atcgaccgcc
      181 aggcgccgac cgtaacccag ctcgccgagc gcggcgcgcg cgtcatcgtc tgttcgcatt
      241 tcgaccgccc caagggcaaa gtcgtgccct ccatgtcgct ggcgccggtg agtgggccgc
      301 tggccaaggc cgtgggccgg ccggtcgcct tcgcttccga ttgcgtcggc aagctcgccg
      361 aggaggcggt cgccaagctc aaggacggcg acgtgctgct gctcgaaaac acccgcttcc
      421 atgcgggcga ggaaaagaac gatcccgaat tggccaagca gcttgccgcg cttgccgatc
      481 tctacgtcaa cgacgccttt tccagcgccc atcgcgccca tgcctcgacc gaaggcgtgg
      541 cgcatctctt gcccagcgcg gcgggacgct cgatggaagc cgaactgacc catctgcaca
      601 aggcgctgaa cgatcccgag cgcccgctga tggcggtgat cggcggcgcc aaagtctcga
      661 ccaagatcgc gctgctggag aatctggtga agcgggtgga gacgctggtg atcggcggcg
      721 ccatggcgaa cactttcctg gcggcccaag gcaagaacat cggcaagtcg ctgcacgagc
      781 acgaccatct ggagaccgcg cgccgcgtga tcgcccagtc ggtcgaaagc ggggtggcgc
      841 tgctgctgcc gaccgacgcc gtggtggcga aggaattcaa ggctggcgcc gcttcgcgca
      901 cggttcccat cgaccaggtc ggcgacgacg aaatgatcct cgacatcggc cccgccagcg
      961 tggcggagtt cgagaaattc ctgcacaaca cgcgcaccgt ggtatggaac ggtccgctcg
     1021 gcgcctttga aacctcgccc tttgaaaagg gcaccgtcgc cgcggcgaag cttgtggccc
     1081 agcgcaccaa ggccggtcag cttctgtcgg tggccggcgg cggcgatacg gtcgcagccc
     1141 tcgccatggc cggggtgaag gacgatttct cctacgtctc gacggcgggc ggcgccttcc
     1201 tcgaatggat ggaaggcctg gtgctgcccg gcgtcgaagc cttgatggtg aagtagaact
     1261 cgcgtcgctt tcctcatgct tcgacaggct cagcatgagg gcgcataaca agccctcacc
     1321 ctgagtcctg agcgtgtcga aggatcgaag ggtgggggct tgtcttatgg agaaccgcca
     1381 tgtccgatgc cgtacagaaa atcctgagct ggtacgaaag cgacaatccg ggcgtgaaga
     1441 ccaacctcgc ccgcatgctc tatcacggcc gcctgggcgg caccggcaaa ctggtcatcc
     1501 tgccggtgga ccaaggttat gagcacggcc ccgaccgctg cttcgtgccc aatccgccgg
     1561 cctacgaccc gcattatcac ttccaactcg cgatcgacgc cgggctttcg gccttcgctt
     1621 cgccgctcgg catgctggaa gcgggcgcgg cgaccttcgc cggacgcatt ccgctgatcc
     1681 tcaaggtcaa cagcgccaac ctgctctatc ccaaggacgg gccgaaaacg caggccgtca
     1741 ccgcgtcggt ccacgatgcg ctgcggctcg gctgcacggc ggtgggcctc accatctatc
     1801 ccggttcgct cagatcctac gacatgttcg aagtggcccg cgatttcgtc gccgaggcga
     1861 aggacgtcgg cctgccgacg gtgatctggt cctatccgcg cggcgaggac atcagcaagg
     1921 acggcgagac cgccatcgac gtggacgctt atgcgacgca gatcgcggcc ctgctcggcg
     1981 cccacatcat caaggtcaag ctgccgacga gcttcgtcga agccaaggat atcgccgagc
     2041 tatacaagaa gggcaacgtc aagctcgaca cccttcccga ccgcgtggcg ctgatcaaga
     2101 aggcggcgct gggcgggcgg cgcatcgtga tcttctccgg cggcggcaaa aaggccgacg
     2161 aggaaatcct cgaagaagcg cgcgccatca aggcgggcgg cggcgacggc tcgatcatgg
     2221 gccgcaacgc cttccagcgc tcgaaggcgg acgccatggc cctgctcgac aaggtgatcg
     2281 agatttataa ggactgaggt gcgcaccaag ttaccttcac ccttcgacaa gctcagggtg
     2341 aaggattagt ttaaccctca tgctgagctc gtcgaagcat gagggccatg accacccgct
     2401 gccgcctgta tttgatcacc ccgcccaagc ttgagccgcg cgcgtttgcg gaaacgctga
     2461 agcgcacgct gggcgcgggc gatgtcgcct cgctgcaatt gcggctgaaa gacgtatccg
     2521 acgaagagat cgtcggcgcg gcggacatct tgatgttcgt cgcccaacaa gccggcgtcg
     2581 ctttcatcct caacgaccgg ccggaccttg ccgccaagat cggctgcgac ggcgtgcatg
     2641 tcggccagga ggacgcttct tacgccgagg cgcgcgccgc gctgggatcg gacaagatcg
     2701 tcggcgtcac ctgccacgcc tcgcgccatc tcgccatcga agccgccgac gcaggcgcgg
     2761 actatgtcgc cttcggcgcc ttcttcccga ccgcgaccaa ggaggcgaag gcgcacgccg
     2821 acttagatat tctccaatgg tggtcggagc tgatggtggt gccttgcgtc gccatcggcg
     2881 ggatcacggt ggcgaacgcc aagccgctgg tggacgcggg ggccgatttc ctcgccgtgt
     2941 cgtcgggcgt gtgggatcat ccggacggtc cggccgccgc cgtgaaggcg ttcaacgttt
     3001 tgttttaatt gaagaatatt attgcttaca cgggcttggc gcgaccgaaa gtccgaccgg
     3061 ggcggtgagc cttggcaccg ccgcaacgaa acgctaccac ccttcatatt gtgtggcctg
     3121 tgccgcgacc tgaagaagag gacgagatga ccaagacttt gcgccccggc gtcgtgaccg
     3181 gcccggacta tctgcaattg ctggccgcct gcaaggaagg cggctatgcg ctgccggccg
     3241 tgaacatcgt cggcaccagc ggcatcaatg cggtgctgga aaccgccgcc aaggcgaagt
     3301 ccgacgtcat cgtccagatg tccaacggcg gcgcccgctt ctatgccggc gaaggcgctc
     3361 ccgatgcgca tcgcgcccgc gtgctgggcg cgacctcggc cgcgcagcat gtgcatctgc
     3421 tggccgcgga atacggcgtc tgcg
//
LOCUS       DASWPU010000573         5913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_72555,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000573 DASWPU010000000
VERSION     DASWPU010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72555"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..1714)
                     /locus_tag="VGC27_12110"
     CDS             complement(263..1714)
                     /locus_tag="VGC27_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY0283355.1"
                     /translation="MGKLTGFLEIERQDRGAEKPDVRKTNFREFVKPLPYGEVGRQAA
                     RCMDCGIPFCHTGCPVNNLIPDWNNLVYREQWRAALETLHSTNNFPEFTGRVCPAPCE
                     TSCTLNIQDNPVTIKTIECQIVDRGWEEGWIKPSIAAEKTGKKIAVVGSGPAGLACAQ
                     QLARAGHGVTLFEKNDRIGGLLRYGIPDFKLEKRFIDRRLVQMQAEGVEFRTGVEVGA
                     AHPVSALLSEYDAVVMAGGAESPRDLRVEGRDLVGIHFAMDYLVQQNKRVAGDEESVA
                     APKGTLSAKGKRVVVIGGGDTGSDCIGTANRQGALSVTQLEILPKPPEKENKGLTWPD
                     WPQKLRTSSSQEEGCARDWSVLTKRALGKDGKIEALECVRVEWKLGADGRMQMIEIPG
                     SAFKIECDLVLLAMGFVGPRKAGLVEQAGVELDPRGNVKAGVQDYKTTTPKIFSCGDM
                     RRGQSLVVWAIHEGRECAREVDKFLMGASELPC"
     gene            complement(1717..>5913)
                     /gene="gltB"
                     /locus_tag="VGC27_12115"
     CDS             complement(1717..>5913)
                     /gene="gltB"
                     /locus_tag="VGC27_12115"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEY0283356.1"
                     /translation="QAIIGWGPNIRDEGAFERKLLAVRKQTQNPLAALARKHKLPAIT
                     HFYMPSFSASTVVYKGLLLAPDVGRFYLDLQNPLTASAIALVHQRFSTNTFPSWKLAH
                     PYRFIAHNGEINTLRGNVNWMNARRRTMESDLLGADLDKMWPLIPHGQSDTASLDNAL
                     ELLIAGGYPIAHAVMMLIPEAWAGNKMMDAKRRAFYEYHAALLEPWDGPAAIAFTDGK
                     QIGATLDRNGLRPARFIVTEDNLVIMASESGVLPVPEEKIKRKWRLQPGKMLLIDLEQ
                     GRIVEDEEIKTQLAAAEPYEEWLNAAQYKLEELPDVANGHVEAAGDASALLDRQQAFG
                     YTQEDLKFFLEPMAKGGEDPVGSMGCDTPIAVLSKKPKLLYNYFKQNFAQVTNPPIDP
                     IREELVMSLVSMIGPRPNLLGHQAGAHKRLEVHQPVLTNADVQKIRSIEELVDGAFRT
                     ATIDCTWPASEGAKGLAGALQHICMQATDAVLADFNILILSDRAVSPDRIPVPAALAT
                     AAVHHHLIRQGLRMQTGLVVETGEAREVHHFCVLAGYGAEAINPYMAFETIEQIRVQA
                     GIALEPADVQKNYIKAIGKGVLKVMSKMGISTYQSYCGAQIFDAVGLSSAFVEKYFTG
                     TATTIEGIGLEDIAEECVVRHRGAYGDNPIYRDMLDVGGNYAFRLRGEEHAWTPDSVS
                     RLQHAVRGNNWPEYKSFAATINEQSERLLTLRGLMRFKLAEEPIPLDEVEPAGEIVKR
                     FATGAMSFGSISREAHTNLAIAMNRIGARSNTGEGGEESERFKPLANGDSMRSAIKQV
                     ASGRFGVTTEYLVNSDDIQIKMAQGAKPGEGGQLPGPKVDKNIARVRHSTPGVGLISP
                     PPHHDIYSIEDLAQLIRDLKSVNPKARISVKLVSEVGVGTVAAGVAKCRADHVTISGF
                     EGGTGASPLTSTTHAGSPWEIGLAETQQTLVMNGLRGRIAVQVDGGIRTGRDVAVAAL
                     LGADEFGFATAPLIASGCIMMRKCHLNTCPVGIATQNPVLRKRFTGTPEHVINYFFFV
                     AEELREIMASMGVRTVNEMIGRVDRLDMTDAVRYWKTQGIDLSKILHMAQPKPGVATY
                     NCEQQNHRLETARDHELIAASRPALEKGKQVVLERTIRNVDRTVGAMLSGEIAKRYGH
                     AGLPEDSIVIRFTGTAGLSFGAFLSRGVSLELYGDANDYVGKGLSGGRIVVCQPKGIS
                     REPTENIIVGNTVLYGAIAGEAYFEGVAGERFAVRNSGAVAVVEGTGDHGCEYMTGGV
                     VVVLGKTGRNFAAGMSGGIAYVYDPDGAFEDLCNRSMVTLEKIAPASGGDDEEMPRRR
                     SVNVADSGMGDPLRFDAERLRILVERHLHYTQSARAKALLDDWDAALGKFVKIVPMDY
                     RRALTDLRAEREAAKAEAAE"
BASE COUNT         1024 a         1937 c         1813 g         1139 t
ORIGIN      
        1 tttatatgtc ccaggcccgt tttaccggcg gaaaacgggc tgccatcata agacgtagag
       61 gcgtgatgcg taacgcaatg gccctcgcgt aagattatga ggtttttgaa gcgcgcggca
      121 cacgcagccg tcaaaaaaga cggccggatt gatattttgc gccatgataa acggcgtctt
      181 tttcaaagcg gggggcgccc gctgcagaat cggccggcga gccatcaaac ttgagggcgt
      241 catcgggcgg tgcgccttgg cactaacacg gcagttcgct cgctcccatc aggaacttat
      301 cgacctcgcg cgcgcactcc cgcccctcgt ggatcgccca caccaccagc gattgcccgc
      361 gccgcatgtc gccgcaggag aagattttcg gtgtggtagt tttgtagtcc tgcacgcccg
      421 ccttcacatt gccgcgcgga tcaagctcca cgcccgcctg ttcgacaagc cccgccttgc
      481 gcggcccgac gaaccccatg gcgagcagca ccagatcgca ttcaatcttg aaggcgctgc
      541 ccggtatttc gatcatctgc atccgaccgt ctgcgcctaa cttccattcc acgcgcacgc
      601 attcgagcgc ctcgatcttg ccgtccttgc cgagggcgcg cttggtcagc accgaccagt
      661 cgcgcgcgca accttcttcc tgcgacgacg aggtgcgcag cttctgcggc cagtccggcc
      721 aggtcagccc cttgttttcc ttttccggcg gcttggggag gatttcgagc tgggtcaccg
      781 acagcgcgcc ttgccggttg gcggtgccga tgcaatccga tcccgtatcg ccgccgccga
      841 tcaccacgac acgcttgccc ttggcgctca acgttccctt cggcgccgcg acggattctt
      901 cgtcgcccgc tacgcgtttg ttctgctgga cgagataatc catcgcaaaa tggatgccga
      961 ccaaatcgcg gccttccacc cgaaggtcgc gcgggctttc cgccccgccc gccatcacca
     1021 ccgcatcata ttcgctgagc agcgccgaga ccggatgcgc cgcgcccact tcgacgccgg
     1081 tgcggaactc cacgccctcg gcctgcatct gcaccaagcg gcggtcgatg aagcgcttct
     1141 ccaacttgaa atcgggaatg ccgtagcgca gcaggccgcc gatccggtcg ttcttttcga
     1201 acaaggtgac gccgtggccg gcgcgcgcaa gctgctgcgc gcaagccagg cccgcgggac
     1261 cggaacccac caccgcgatc ttttttccgg ttttttcggc cgcaatcgaa ggcttgatcc
     1321 agccctcttc ccagccgcga tcgacgatct ggcattcgat ggttttgatc gtgaccggat
     1381 tgtcctggat gttgagcgtg cagctcgtct cgcagggcgc ggggcagacg cggcccgtga
     1441 actccggaaa attgttggtc gagtgcagcg tttccagcgc cgcgcgccac tgctcgcgat
     1501 agaccaggtt gttccagtcc gggatcagat tgttgaccgg gcagccggtg tggcagaaag
     1561 gaatgccgca atccatgcag cgcgccgcct gcctgccgac ttcgccgtaa ggcagcggtt
     1621 tgacgaactc gcggaaattc gtcttgcgta catcaggctt ctcggcgccg cgatcctgac
     1681 gctcgatttc cagaaagccg gtgagcttgc ccatgtctat tccgcagcct cggctttggc
     1741 cgcttcgcgc tcggccctca aatcggtcaa ggcgcggcga taatccatcg gcacgatctt
     1801 cacgaatttg cccagcgccg catcccaatc gtcgagcagc gccttggcgc gcgcgctttg
     1861 cgtatagtgc aggtggcgtt cgaccaggat gcgcagccgt tccgcgtcga agcgcaaggg
     1921 atcgcccatg ccggaatccg ccacattgac ggaacgccgc cgcggcatct cttcgtcgtc
     1981 gccgccggac gcgggagcga tcttctccag cgtcaccatc gagcggttgc agagatcctc
     2041 gaacgccccg tccgggtcgt agacataggc gatgccgccc gacatgcccg ccgcgaaatt
     2101 gcgcccggtt ttgccgagca cgaccacgac gccgccggtc atatattcgc agccatgatc
     2161 gccggtgcct tcgaccaccg cgaccgcgcc cgaattgcgc acggcaaaac gctcgcccgc
     2221 cacgccctcg aaataggcct cgccggcaat cgcgccgtaa agaaccgtgt tgccgacgat
     2281 gatgttctcg gtaggctcgc ggctaatgcc cttgggctgg cacaccacga tgcgtccccc
     2341 ggaaagcccc ttgccgacat agtcgttggc gtcgccgtag agttccaagc tgacgccgcg
     2401 cgacaggaag gcgccgaagc tgaggcccgc cgtgccggtg aagcggatga cgatcgaatc
     2461 ttctggcagc ccggcatggc cgtagcgctt ggcgatttcg cccgacagca tggcgccgac
     2521 ggtgcggtcc acattgcgta tggtccgctc cagcaccacc tgcttgccct tttcgagcgc
     2581 cggccgcgag gccgcgatca attcgtgatc gcgcgcggtc tccaagcgat gattctgctg
     2641 ctcgcaattg tacgtggcca cacccggctt gggctgcgcc atgtgcagga ttttcgacag
     2701 gtcgatgcct tgcgtcttcc agtagcgtac cgcgtccgtc atatcgagcc ggtcgacgcg
     2761 cccgatcatc tcgttgacgg tgcgcacccc catcgacgcc atgatctcgc gcaattcctc
     2821 agcgacgaag aagaaatagt tgatcacatg ctccggcgtg ccggtgaaac gcttgcgcag
     2881 caccgggttc tgcgtcgcga tcccgaccgg acaggtgttg agatggcatt tgcgcatcat
     2941 gatgcagccc gaggcgatca gcggcgcggt ggcgaagccg aattcgtcgg cgcccaagag
     3001 cgcggcgacg gccacatcgc gcccggtgcg aatgccgccg tccacctgca cggcgatgcg
     3061 cccgcgcaag ccgttcatca ccaaggtctg ctgggtttcc gccaggccga tctcccacgg
     3121 gcttcccgca tgggtggtcg aggtcagcgg cgaggctccg gtgccgcctt caaaaccgga
     3181 aatcgtcaca tgatcggccc ggcatttggc gacgcccgcc gccaccgtgc cgacgccgac
     3241 ctcggacacc agtttcaccg aaatgcgcgc cttggggttg acgctcttca aatcgcggat
     3301 caactgcgcc agatcctcga tggaataaat gtcgtgatgg ggcggcggcg agatcaagcc
     3361 cacgcccggc gtcgaatggc gcacacgggc gatgttcttg tccaccttgg gaccgggcaa
     3421 ctgcccgccc tcgcccggct tggccccttg cgccatcttg atctggatgt cgtcggagtt
     3481 gacgagatac tccgtggtca cgccgaaacg gcccgacgcc acctgcttga tggcggagcg
     3541 catggaatcg ccgttggcga gcggcttgaa gcgttcggat tcctcgccgc cctcgccggt
     3601 gttggagcgc gccccgatgc ggttcatcgc aatcgccagg ttggtgtgcg cctcgcggga
     3661 aatcgaaccg aaactcatcg ccccggtggc aaagcgcttg acgatctccc ccgccggttc
     3721 gacctcgtcg agcgggatcg gctcttccgc aagcttgaac cgcatcagcc cgcgcaaggt
     3781 caaaagccgc tcgctctgct cgttgatggt ggcggcgaac gatttatatt ccggccagtt
     3841 gttgccgcgc acggcatgtt gcagccgcga caccgagtcc ggcgtccagg cgtgctcttc
     3901 gccgcgcagg cggaaagcgt aattgccgcc gacatccagc atgtcgcgat agatcggatt
     3961 gtcgccatag gcgccgcgat ggcgcacgac gcattcctcg gcgatgtcct ccaggccgat
     4021 tccttcgatg gtcgtcgccg tgccggtgaa gtatttctcg acaaaggccg aggacagccc
     4081 caccgcgtcg aaaatctgcg cgccgcaata ggattgatag gtcgagatgc ccatcttgga
     4141 catcactttg agcacgccct tgccgatggc cttgatgtag ttcttctgca catcggccgg
     4201 ctcaagcgca attccggcct ggacgcgaat ctgctcgatc gtctcgaagg ccatataggg
     4261 attgatcgcc tcggcgccgt acccggccag aacgcagaaa tgatgcactt cgcgcgcttc
     4321 gccggtctcc accaccagcc cggtctgcat gcgcaggccc tggcggatca gatggtgatg
     4381 caccgccgcc gtggccagcg cggcgggaac cggaatacgg tccggcgaaa ccgcccgatc
     4441 cgacagaatg aggatattga agtcggccag caccgcatcg gtcgcttgca tgcagatgtg
     4501 ctgcaaggcg cccgccagcc ccttggcgcc ttccgaggcg ggccaggtgc agtcgatggt
     4561 cgcggtgcgg aaggcgccgt cgaccagttc ctcgatggaa cggatcttct gcacgtcggc
     4621 gttggtcagc accggctggt gaacctccag ccgcttgtgc gcgcccgcct ggtggccgag
     4681 cagattgggc cgcggcccga tcatcgacac caggctcatc accagttcct cgcggatcgg
     4741 atcgatcggc gggttggtga cctgggcgaa attctgcttg aagtaattgt atagcagctt
     4801 gggcttcttc gagagcacgg cgatcggcgt atcgcagccc atcgaaccga cgggatcctc
     4861 gccgcctttc gccatcggct cgaggaagaa tttcaaatct tcctgggtgt agccgaaggc
     4921 ctgctggcga tcgagcagcg cggacgcatc gccggccgct tccacatgcc cgttcgccac
     4981 atcgggcagt tcttccagct tgtactgcgc cgcgttcagc cattcttcgt agggttcggc
     5041 ggcggcgagc tgggttttga tctcttcgtc ttcgacgatg cggccctgtt cgagatcgat
     5101 cagcagcatc ttgccgggct gcaagcgcca tttgcgcttg atcttctctt ccggcaccgg
     5161 cagcacgccg gattccgacg ccatgatgac gagattgtcc tcggtgacga tgaagcgggc
     5221 gggacgaagc ccgttgcggt cgagcgtggc gccgatctgt ttgccgtcgg taaaggcgat
     5281 ggcggcgggc ccgtcccacg gttcgagcag cgccgcatga tattcgtaaa aggcgcggcg
     5341 cttggcatcc atcattttgt tgccggccca ggcttcgggg atcagcatca tcaccgcatg
     5401 ggcgatggga tagccgcccg cgatcaaaag ctccaaggca ttgtccaggc tcgccgtgtc
     5461 ggactggccg tgcggaatca gcggccacat cttgtcgaga tcggcgccca gcaaatccga
     5521 ttccatggtg cgccggcgcg cgttcatcca gttgacgttg ccgcgcaatg tgttgatctc
     5581 gccgttatgg gcgatgaaac gatagggatg ggccagcttc cacgatggga aggtgttggt
     5641 cgagaagcgc tgatgcacca gcgcaatcgc cgatgcggtg agcggatttt gcaggtcgag
     5701 atagaagcgg ccgacatcgg gagccagcaa gaggccctta tagaccaccg tgctcgccga
     5761 aaagctcggc atatagaaat gggtgatggc gggcagcttg tgcttgcggg cgagcgccgc
     5821 cagcggattc tgcgtctgct tgcggacggc aagcagcttg cgctcgaagg caccctcgtc
     5881 gcggatgttc ggcccccagc cgatgatcgc ctg
//
LOCUS       DASWPU010000574         5779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_47971,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000574 DASWPU010000000
VERSION     DASWPU010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47971"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..520)
                     /locus_tag="VGC27_12120"
     CDS             complement(239..520)
                     /locus_tag="VGC27_12120"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY0283357.1"
                     /translation="MKKLDAAAIAAFLREFPEWREGRDAITRLFQFPDFNRAFGFMTR
                     VALLAEKLDHHPEWFNLYNKIEVTLSTHDAGGVTEKDIAMAKAMEEYAG"
     gene            545..943
                     /locus_tag="VGC27_12125"
     CDS             545..943
                     /locus_tag="VGC27_12125"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY0283358.1"
                     /translation="MDWMGMEAPSIDEIERLARQAYAKLPENFRALCGDVLFMVQEFP
                     GNDVIRDLELESEFDLLGLYQGSTLADRVGNRDRPEPTMIFLYRRPIIDYWAEHGGSL
                     SAIVRHVVVHEIGHHFGLSDKDMEAIEEGE"
     gene            complement(1088..1618)
                     /gene="thpR"
                     /locus_tag="VGC27_12130"
     CDS             complement(1088..1618)
                     /gene="thpR"
                     /locus_tag="VGC27_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY0283359.1"
                     /translation="MRLFVAIALPDEVARSLAALQAGVPGARWQAREQLHLTLRFIGE
                     VDGRDAASIDDALAAITAPAFSLELKGVGAFGGKRPHALWAGVVPSEPLLHLQRKIES
                     ALQRIGLEAEGRKYTPHVSLARLKGTPNGHVVDFLTDHALYASPPFTASGFILYSSLL
                     TPNGSIYRAEKAYRLK"
     gene            complement(1618..3156)
                     /gene="murJ"
                     /locus_tag="VGC27_12135"
     CDS             complement(1618..3156)
                     /gene="murJ"
                     /locus_tag="VGC27_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY0283360.1"
                     /translation="MFKRLLTVAGFTALSRVTGFIRDVIMAFVLGAGPMSDAFLVAFR
                     LPNNFRAIFGEGAFNAAFLPRYAWVRTQEGPEAAARFADDIFSWQMAFQLVLLAAALA
                     AMRYIIAALAPGFGAHPGQMELATALARIAFPYLIMTVAAVQLSSMLNAAGKFAAAAA
                     WSIFLNVAMVATLLSARWFPNAAYAAAWGVFLAGILQFAFIVWAAAKERLRLRFHWPR
                     WTPQVKEFLLALGAATIGSASVEISLFIDSWIASFLPSGNLTALYYADRINQLPMGVL
                     GIALGTVLLPEMSNLLAKNRTADANAAQNRAAAMGLLLTLPFVAAFFAIPQTIMHTIF
                     AHGAFVASAADISAAALMAYGIGLPAFVLVRVTSATFYARGDTVRPVRATMLAVAVNI
                     VLKFVLVWGAGLGAVGIALGTAIGAWANVAMLIWMASHRDLLKVSPNFWRALGPILLA
                     AAAAGVGALCGEELGGRIVAHPGLLHDAAALGTAIAFGGACYLLVVVAFRRVLPLGRF
                     AGVQ"
     gene            3387..4157
                     /locus_tag="VGC27_12140"
     CDS             3387..4157
                     /locus_tag="VGC27_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY0283361.1"
                     /translation="MADYDDQIIRGQAAEVSVIDAGLRAYMLRVYNYMLVGLVLTGLT
                     AYSVYSVAVTADPAAAAATMQNGVMLTSFGVTLFGTPLKWLFMLAPLGAVLYINARLR
                     HMSVGGAQSAFWIFAGLMGISFSTIFMVYAAASIAQVFFITAAAFGGLSLYGYTTKRD
                     LSPFGSFLIMGVWGLLIAMVVNIFLQSPMMMWVMSVAGVGIFAGLTAYDTQRIKEMYS
                     VGDDGTLAAKKSIMGALALYLDFINMFQFLLFLMGGRR"
     gene            complement(4456..4821)
                     /locus_tag="VGC27_12145"
     CDS             complement(4456..4821)
                     /locus_tag="VGC27_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283362.1"
                     /translation="MIDTRILQRAAIIGTVLQIALSVAGYFFPWVAIHLFMFGRMMIS
                     ASAGYLYGMEFGRGYAISALGGAVAGGLCVVPAIALSVLLGDTGASMVAVGTGISILT
                     GGVGGAFGQIAANLRKLGY"
     gene            4936..>5779
                     /locus_tag="VGC27_12150"
     CDS             4936..>5779
                     /locus_tag="VGC27_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0283363.1"
                     /translation="MIEIEGLTKRFGTVTAVAGLTLKVGKGEVLGFLGPNGAGKSTTM
                     KMVTGFLAPSEGRIRVCGHDVETDTIRAQASICYLPEGAPSYGDMTASQFLAFIAEVR
                     GFKGAEAKTRVSAAVDKTEIAPVLDQPIETLSKGFKRRVGLAQAILHDPPVLIMDEPT
                     DGLDPNQKHSVRTLIRAMATEKAIVISTHLLEEVEAICTRAVIIDRGAIVADGSPASL
                     LERSRYHNAVTMVLPENEADAAAGKLKALAPVAGVERSQRRDGAARLTAFPKSGALLI
                     EAISA"
BASE COUNT         1115 a         1878 c         1792 g          994 t
ORIGIN      
        1 gccggaggac gcgttctgga aagtcggcgt tttcgtcacc aacgtgctcg atcagaagtt
       61 catcaaggac gccggcaaca gcggcgacaa tctcggcatc ccgaccttca tcgccggcga
      121 gccgcgcttc ttcggcatga gtgtttcctt caaaacgcga taaatgggac ctccctctcc
      181 cgcaatcggg ccgccgtggc gtcacggcgg cccttttttc gccgcatgcg gttgcggttc
      241 agccggcata ctcctccatc gccttggcca tcgcgatgtc cttctcggtc acgccgcccg
      301 cgtcgtgggt ggagagcgtc acctcgatct tgttgtagag gttgaaccat tcgggatgat
      361 ggtcgagttt ctccgccagc agggccacgc gggtcatgaa cccgaacgcc cggttgaagt
      421 ccgggaactg aaacaggcgg gtgatcgcat cgcggccctc gcgccattcg ggaaattccc
      481 gtaagaaggc ggctatagcg gcggcatcga gcttcttcat gttcgctccc ttgagttaga
      541 ggccatggac tggatgggca tggaagcacc ttccattgac gagatcgagc ggctcgcgcg
      601 ccaagcctat gccaagctgc ccgaaaattt ccgcgctttg tgcggcgacg tcctgttcat
      661 ggtgcaggag tttcccggca acgacgtcat ccgcgacctg gaactggaat ccgaattcga
      721 cctgctcggc ctgtaccagg gctcgaccct ggccgaccgc gtcgggaacc gcgaccggcc
      781 ggaaccgacg atgatcttcc tctaccggcg gccgatcatc gattattggg cggaacatgg
      841 cggctcgctc agcgccatcg tgcgccacgt ggtcgtgcac gagatcggcc accatttcgg
      901 tctctcggac aaagacatgg aagcgatcga ggagggggag tgaagtctag cgcgcgatcc
      961 atcaaaatga gatcgtgcca tactcatgct tcgacaagct cagcatgaag gattaggtac
     1021 tcccctcacc ctgagcttgc cgaagggcga caaggaacaa ttcaatcgga ctggattatg
     1081 ctttggccta cttcagccgg tacgccttct cggcccggta aatacttcca ttcggcgtca
     1141 gcaggctgga gtagagaatg aatccgctcg ccgtgaaggg cgggctcgca tagagcgcat
     1201 ggtcggtgag gaaatccacc acatgcccgt tcggcgttcc cttgagcctg gcgagggaaa
     1261 catgcggcgt gtatttgcgg ccttccgctt ccaggccgat gcgctgcagc gcgctttcga
     1321 tcttgcgttg cagatggagg agtggctcgc tggggacgac tcccgcccat aaagcatggg
     1381 gacgcttgcc gccgaacgca cccacgcctt tcaattcaag cgaaaaggcg ggcgcggtaa
     1441 tcgccgccag cgcgtcgtcg atggaggcgg cgtcgcgccc gtccacctcg ccgatgaagc
     1501 gcagcgtcag gtgaagctgc tcgcgcgcct gccagcgtgc gccgggcacg cccgcctgaa
     1561 gcgccgccag ggaccgcgcc acctcgtcgg gcagcgcgat ggccacgaac agccgcatca
     1621 ctgcaccccc gcaaaacgtc cgagcggaag cacgcggcgg aaggcgacga ccaccagcag
     1681 atagcaggcg ccgccgaaag caattgccgt ccccagcgcc gccgcgtcat gcagcagccc
     1741 gggatgcgcg acgatccgcc cgcccaattc ttcgccgcac agcgcgccca cgccggctgc
     1801 cgccgccgcc agtaggatgg ggccgagggc gcgccagaaa ttcgggctga ctttcagaag
     1861 gtcgcggtgc gacgccatcc agatcagcat ggcgacgttc gcccaggcgc cgatcgccgt
     1921 gccgagcgcg atgccgaccg cgcccaaccc ggcgccccac accagcacga atttgagcac
     1981 gatattgacg gcgaccgcca gcatcgtggc gcgcaccggg cgcaccgtgt cgccgcgcgc
     2041 gtagaacgtc gcactggtga cgcgcaccag cacgaaggcc ggcaggccga tgccgtaagc
     2101 catcaaagcg gcggcggaaa tatcggccgc cgatgccaca aaggcgccgt gggcgaaaat
     2161 cgtatgcatg atggtctgag gtatggcgaa aaaggcggcc acgaacggca aggtcagcaa
     2221 gaggcccatc gcggcggcgc ggttctgcgc ggcgttggcg tcggcggtcc tgttcttcgc
     2281 cagcagattc gacatttccg gcaacaacac ggtgccgagc gcgataccca gcacccccat
     2341 cggcaattgg ttgatgcggt cggcgtaata aagcgccgtc aggttgccgg aaggcaggaa
     2401 gctggcgatc caactgtcga tgaaaaggct gatttcgacg ctggccgatc cgatggtcgc
     2461 cgcgcccagc gccagaagga actccttgac ctgcggcgtc cagcgcggcc aatggaaccg
     2521 caggcgcagc cgctctttcg ccgccgccca gacgatgaat gcaaattgca gtatgcccgc
     2581 caggaatacg ccccaggccg ccgcataggc cgcattcgga aaccagcgcg ccgagagcag
     2641 cgtcgccacc atcgcgacgt tcaggaagat cgaccaggcc gcggccgcgg cgaacttccc
     2701 cgccgcgttc agcatcgacg aaagctggac ggcggccacc gtcatgatga ggtaggggaa
     2761 ggcaatccgc gccagggcgg tcgccagttc catctgcccc ggatgggcgc caaagcccgg
     2821 agccagcgcc gcgatgatgt agcgcatcgc cgccagcgcc gcggccagca gcacgagctg
     2881 gaacgccatc tgccaggaga agatatcgtc ggcgaagcgc gccgccgctt ccgggccttc
     2941 ttgcgtgcgc acccaggcat agcgcggcag gaaggcggcg ttgaaggcgc cctcgccgaa
     3001 aatggcgcgg aaattgttgg gcagccggaa ggcgacgagg aaggcgtccg acatcggccc
     3061 cgcgcccagc acaaaagcca tgatgacgtc gcggatgaaa cccgtgaccc gcgagagcgc
     3121 ggtaaagccg gcaacggtga gaaggcgttt gaacatcgga ggtttcagat cacttctcag
     3181 gaatcgcccg cgcccgcctc gaaggctccg gcatagacga tttctctggt gcccaacaga
     3241 gcgcaggatg accgattcgt catgttttcc gcctcaataa ccccatataa tgccggacga
     3301 atcccagcat accttgatcc tggggcgccc aagcacgata ttaggggtgt gatgcgaccc
     3361 ttgcgggccg aaaggaagga ttatccatgg cggactacga cgatcagatc attcgcggcc
     3421 aagcagccga agtaagcgtc atcgacgcag ggctgcgcgc ctacatgttg cgcgtttaca
     3481 actacatgct ggtgggcttg gtcctgacgg gactgaccgc ctacagcgtc tattcagtgg
     3541 cggttaccgc cgacccggca gccgccgcgg ccacgatgca aaacggcgtg atgctgacga
     3601 gcttcggcgt gacgctgttc ggcaccccgc tgaaatggct gttcatgctg gctcccctag
     3661 gggccgtgct ctacatcaac gcccggctgc gccatatgag cgtcggcgga gcgcaatcgg
     3721 cgttctggat cttcgccggc ctgatgggca tctcgttttc gaccatcttc atggtctatg
     3781 cggcggcgag tattgcccaa gtgttcttca tcacggcggc ggcgttcggc gggttgagcc
     3841 tctacggcta caccaccaag cgcgatctct cgccgttcgg ctcgttcctg atcatgggcg
     3901 tatggggcct cctaatcgcc atggtcgtga acatcttctt gcagtcgccg atgatgatgt
     3961 gggtcatgtc ggttgccggc gtcggcatct tcgccgggct gacggcctat gacacccagc
     4021 gcatcaaaga gatgtattcc gtcggcgacg acggcacgct ggcggccaaa aagtcgatca
     4081 tgggcgcgct ggcgctctat ctcgacttca tcaatatgtt ccagttcctg ctcttcctga
     4141 tgggcggccg ccgctaggcg caccgccacg ccgaaacgta aagccccgat ggcgacatcg
     4201 gggctttttc gtttattcgc gcggggggtc aatttcgtct aattcgcgac cgcacaatgt
     4261 cgcagccttg aatctcttaa atattcttca tactaagtca caaaagcgcc acgtttggac
     4321 cacggcaggg cctcactcgg cggcgaaatt ctccccgtcg gctggcttgc gttgaaggac
     4381 cctcctgcga cctgtcaagc cccccagccc ccacagtccg gggcggtcca gattggccag
     4441 ccgacctctt cttcttcaat atcccagctt gcgcaagttc gcggcgatct ggccgaaggc
     4501 gccgccgacg ccgccggtca ggatggaaat gccggtgccg accgccacca ttgaggcgcc
     4561 ggtgtcgccc agcagcaccg acagcgcgat ggccgggaca acgcatagcc cgccggcgac
     4621 ggccccgccc aatgcgctga tcgcataacc cctgccgaat tccattccgt agaggtaacc
     4681 ggcgctcgcc gaaatcatca tgcggccgaa catgaagaga tggatcgcga cccaagggaa
     4741 gaaataaccc gccacggaca gggcgatttg caggacggtc ccgataatcg ccgcgcgttg
     4801 cagaatgcgg gtgtcgatca tggcttttgc cttccttttc ccgggccgat gtctcattcg
     4861 taaccttttc ggggttgcga gccaaaggcg atttctctaa gggattcgag cccttcaccc
     4921 cgccgcttgc cgcccatgat cgaaatcgag ggactgacca aacgcttcgg caccgtcacg
     4981 gcggtggccg gactcaccct caaggtcggc aaaggcgagg ttttgggctt tctcggcccc
     5041 aatggcgcgg gcaaatccac caccatgaag atggtgacgg gttttctggc gcccagcgaa
     5101 ggcaggatcc gcgtctgcgg ccacgacgtc gagaccgaca ccatccgcgc ccaggcctcg
     5161 atctgctatc tgcccgaagg cgcgccctcc tacggcgaca tgacggccag ccagttcctc
     5221 gccttcatcg ccgaagtgcg cgggttcaaa ggggccgagg ccaagacacg ggtgagcgcc
     5281 gcggtggaca agaccgagat cgctcccgtt ctcgaccagc ccatcgaaac cctgtccaag
     5341 ggctttaagc gccgcgttgg cctggcgcag gcgattctgc acgatccgcc ggtgctgatc
     5401 atggacgagc cgaccgatgg gttggacccc aaccagaaac attccgtgcg cacgctgatc
     5461 cgcgccatgg cgaccgaaaa ggccatcgtc atctcgacgc atctgctgga agaagtggaa
     5521 gcgatctgca cccgcgcggt gatcatcgat cgcggcgcca tcgtcgccga tggctcgccg
     5581 gcctcgctgc tggagcgctc gcgctatcat aatgccgtga ccatggtgct gcctgaaaac
     5641 gaggccgacg cggcggcagg caagctcaag gcgctcgccc cggtggccgg cgtcgaaaga
     5701 agccagcgcc gggacggcgc ggcgcggctc accgccttcc ccaagagcgg cgcgctgctg
     5761 atcgaggcca taagcgcgc
//
LOCUS       DASWPU010000575         5626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_115707,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000575 DASWPU010000000
VERSION     DASWPU010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115707"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VGC27_12155"
     CDS             complement(<1..471)
                     /locus_tag="VGC27_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEY0283364.1"
                     /translation="MRLLLASESPQRKALLAQAGITPDAIRAAAIDETPLKDELPRLH
                     ALRLAREKACAAAANLAGAPMLVLGADTVVACGRRILPKAEDAETVRACLKLLSGRRH
                     QVITAVAVVLPDKSIRTRIVVTSVAFRRLSAAEIETYVQSREGEGKAGGYAIQGR"
     gene            complement(479..697)
                     /gene="infA"
                     /locus_tag="VGC27_12160"
     CDS             complement(479..697)
                     /gene="infA"
                     /locus_tag="VGC27_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY0283365.1"
                     /translation="MPKEELLEFEGVVSELLPNATFRVKLQNGHEIIAHTAGKMRKNR
                     IRVLTGDKVMVEMTPYDLTKGRITYRFK"
     gene            complement(999..1481)
                     /locus_tag="VGC27_12165"
     CDS             complement(999..1481)
                     /locus_tag="VGC27_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEY0283366.1"
                     /translation="MGEAPLYCLTGVTLDERTSVRRTHEIEHERDIALVDLLESNNFH
                     PVGSHGGPYHLVLGVDDKHLTLDIRLASGEAHGKLMLSLTPFRRIIKDYFRLCESYFK
                     AVGGAHSAGQIESLDMARRGLHDEGSELLRERLKGKVDIDMDTARRLFTLICVLHLKG
                     "
     gene            complement(1485..2789)
                     /gene="hisD"
                     /locus_tag="VGC27_12170"
     CDS             complement(1485..2789)
                     /gene="hisD"
                     /locus_tag="VGC27_12170"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY0283367.1"
                     /translation="MTHWLDARAADFASGFNAFLALKREADKDVAASVRAIIAEVRER
                     GDAALIDFGKRFDKADLTADTLRLSGKEIAAAQSACDSQALAALDTAAARIEDYHRRQ
                     IPSDVSYTDELGARLGWRWTAMDSVGLYVPGGTANYPSSVLMNAIPAFVAGVKRIVMV
                     TPASGGAVNPLTVEAARRVGIGEIYRVGGAQAVAALAYGTETIAPVDKIVGPGNAYVA
                     AAKREVFGRVGIDSVAGPSEVLIVADGANNPDWIAADLLSQAEHDAAAQSILITDDAA
                     FAKQVEEAVARALALLPRKDIAGASWRDYGAIIVVAELNDAAALVDRIAPEHLEIATA
                     DPEALLKRVRHAGAIFLGRFTPEAMGDYIAGPNHVLPTGRTARFSSGLSVFDFIKRST
                     LLYCTPYVLAKLGPEAIALAQAEGLDAHARSIAARLNYPAGG"
     gene            complement(2791..3234)
                     /locus_tag="VGC27_12175"
     CDS             complement(2791..3234)
                     /locus_tag="VGC27_12175"
                     /inference="COORDINATES: protein motif:HMM:NF022612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEY0283368.1"
                     /translation="MSTRAPVLAAMDAEDLQVISARLQDAVARLGDLVYLPKKRRFVA
                     LFNRFKWESGKRENLRVRAGLHFDGVLSVKSRNLKLGAPEAVVSLLAVRFAPRAEDDP
                     SGTVELVFAGGGAMMLEVECLEAGLVDLGGDWAARGRPEHENEEY"
     gene            complement(3231..4505)
                     /gene="murA"
                     /locus_tag="VGC27_12180"
     CDS             complement(3231..4505)
                     /gene="murA"
                     /locus_tag="VGC27_12180"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY0283369.1"
                     /translation="MDKIVIRGGVRLKGEIPISGAKNAALPLMAACLLTDAPLTLTNV
                     PKLADVAFMADLLRHLGADAEWAPEAAMGGRYRLCAADLTDTTADYDIVRKMRASFLV
                     LGPLLARAGRAKVSLPGGCAIGARPVDLHLKALEALGAVVELADGYVIASAPQGLKGA
                     LIEFPSVSVGATEHVLMAATLAEGRSTIANAAREPEIDDLGHCLIAMGARISGLGTST
                     IVVEGVKSLHAAEHSVLPDRIETGTYAMAVAIAGGEVELTGTRADLIGSIVALLQAAG
                     AQVTETNRGLSVARNGVRPISVSAATAPFPGFPTDLQAQFMALMATAEGTSTIKETIF
                     ENRFMHVPELLRMGADIKIEGDTAVVSGVPRLKGAPVMATDLRASSSLVLAGLGAEGE
                     TAVNRVYHLDRGFERLEEKLGRVGAEIERLPA"
     gene            complement(4595..4783)
                     /locus_tag="VGC27_12185"
     CDS             complement(4595..4783)
                     /locus_tag="VGC27_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283370.1"
                     /translation="MAKPGKSAERRERLAAALRENLKRRKARLRAGAAEEAETPAEAA
                     VHSRKLPAAAPKGPSKPG"
     gene            4866..4940
                     /locus_tag="VGC27_12190"
     tRNA            4866..4940
                     /locus_tag="VGC27_12190"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4898..4900,aa:Thr,seq:ggt)
     gene            complement(5041..>5626)
                     /locus_tag="VGC27_12195"
     CDS             complement(5041..>5626)
                     /locus_tag="VGC27_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase family protein"
                     /protein_id="HEY0283371.1"
                     /translation="VKGLTVAIQGLGKVGMGVAKRLHEEEAKLIVADINEDLVRRAAD
                     AFRARIASPDDVVTVECDVLSPNALGAILNEKTIPRLHARVVAGGANNQLARAEHGAM
                     LTERGILYAPDYAINGGGIIRVAGQILGWSDAEIKRRTLAIADTLTAIFRRADAEKRP
                     TSVIADAIAEERMAGGGGPAKSAEQAAPMAGLQI"
BASE COUNT          930 a         1914 c         1816 g          966 t
ORIGIN      
        1 gcggccctgg atggcgtagc cgcccgcctt gccctcgccc tcgcggcttt ggacataagt
       61 ttcgatctcg gcggcggaca agcggcgaaa ggcgacggac gtcacgacga tccgtgtacg
      121 gattgacttg tcgggcagga cgacggcgac ggcggtgatg acctgatggc ggcggcccga
      181 gagcagcttc aggcaggcac gcaccgtttc ggcgtcttcc gccttgggca ggatgcgccg
      241 cccgcaggcc accaccgtgt cggcgccgag caccagcatg ggcgcgcccg cgagattcgc
      301 ggcggcggcg caggcctttt cgcgcgccag ccggagcgca tggagccgcg gcaactcgtc
      361 tttcagaggg gtttcgtcga tggcggcggc gcggatcgca tcgggcgtaa tgccggcctg
      421 ggccagcagc gccttgcgct gcggactttc gctcgccagc agcaggcgca ccggtgaatc
      481 atttgaagcg ataggtgata cggcctttcg tgaggtcata cggcgtcatc tccaccatga
      541 ccttgtcgcc cgtcagcacg cggatgcggt tcttgcgcat cttgccggcc gtatgggcga
      601 tgatctcgtg cccgttctgc aattttacgc gaaaggtcgc gttgggcaga agctcgctga
      661 ccacgccttc gaattcgagg agttcttcct ttggcattcg ccctctgggt taatatgcaa
      721 atccggccgg gagaatgcac cgaaatcggc caaaagcaag ggtttacgag gtcctatccc
      781 cgagggtaga gcgaagtgcc tcctgatgga aacgtctgtc attcccggcc gagcatcgcg
      841 tcagcgacgt gaggggaagg gaatccatgg gtttgcgcag gtttctggat gcccttccct
      901 cgcccgtctt cgcgtccaca cgctggcgct atggaccgca gcttcgacgc ggctcgccgg
      961 gcacgacagc atcgattcca cgcgatcctc cccccggctc atcccttcaa atgcagcacg
     1021 cagatcagcg taaaaagccg ccgggccgtg tccatatcga tgtccacctt gcccttcagc
     1081 cgttcgcgca acagttcgga gccttcgtcg tgcaggccgc gccgggccat gtcgagggac
     1141 tcgatctggc ccgcggaatg ggcgccgccg accgccttga agtagctttc gcagagccgg
     1201 aaatagtcct tgatgatgcg gcggaagggc gtcagcgaca gcatcagctt gccgtgcgct
     1261 tcgccgctgg caaggcggat gtccaaggtc aggtgtttgt cgtccacgcc cagcaccaga
     1321 tgataggggc cgccgtgcga accgaccggg tggaaattgt tcgactccag cagatcgaca
     1381 agcgcgatgt cgcgctcgtg ttcgatctcg tgcgtgcggc gcacggaggt gcgttcgtcc
     1441 agggtcacgc cggtcagaca ataaagcggc gcctcgccca tcgctcaacc accggcgggg
     1501 taattcagcc tggcggcgat ggagcgcgcg tgcgcgtcca gcccttcggc ctgcgcgagc
     1561 gcaatggcct ccggcccaag ctttgcgaga acgtaaggcg tacaataaag caaggtcgaa
     1621 cgtttgatga agtcgaacac cgaaaggccc gaggagaacc gcgcggtacg gcccgtcggc
     1681 aaaacatggt tgggcccggc gatgtagtcg cccatcgcct cgggcgtgaa gcggccgagg
     1741 aagatggcgc cggcatgacg cacgcgcttg agcagcgcct cgggatcggc ggtggcgatc
     1801 tccagatgct cgggagcgat ccggtccacc agcgccgccg cgtcgttcaa ttccgcgacc
     1861 acgatgatcg cgccgtagtc gcgccagctc gccccggcga tgtccttgcg cggcaacagc
     1921 gccagcgcgc gggcgacggc ttcctccacc tgcttggcga aggcggcatc gtcggtgatc
     1981 aggatgcttt gggccgcagc gtcgtgctcg gcttgcgaca acaaatcggc cgcgatccaa
     2041 tcgggattgt tggcgccgtc ggcgacgatc agaacctcgg acggtccggc gacggaatcg
     2101 atgccgaccc ggccgaagac ctcgcgcttg gccgccgcca cataagcgtt gccggggccg
     2161 acgatcttat cgacgggcgc gatggtttcg gtgccatagg cgagcgcggc caccgcctgc
     2221 gccccgccga cgcggtagat ttcgccgatg cccacgcgcc gcgcggcttc gacggtcaag
     2281 ggattgacgg cgccgccgct cgccggcgtc accatgacga tgcgcttcac gcccgccacg
     2341 aaggcgggaa tggcgttcat caggaccgaa ctgggatagt tcgccgtgcc gccgggcaca
     2401 tagagaccga cgctgtccat cgccgtccag cgccaaccca gccgcgcgcc cagctcgtcg
     2461 gtgtaggaga cgtcggaggg aatctggcgg cggtgataat cctcgatgcg cgccgccgcg
     2521 gtatcgagcg ctgccaacgc ctggctgtcg cacgcgcttt gcgcggcggc gatttccttg
     2581 ccgctcaggc gcagcgtgtc cgccgtgagg tccgccttgt cgaagcgctt gccgaaatcg
     2641 atcagcgccg catcgccgcg ttcgcgcacc tcggcgatga tcgcacgcac ggaggccgcg
     2701 acgtccttat ccgcttcccg cttgagagcg agaaacgcgt taaagccgct tgcgaaatcc
     2761 gcggcgcgcg catcgagcca atgcgtcatc tcagtactct tcgttctcgt gttcgggacg
     2821 gccgcgcgcc gcccagtcgc cgccgagatc gaccaagccg gcttccaaac attcgacttc
     2881 gagcatcatc gcaccacctc ccgcgaagac aagctccacg gtgccggagg gatcgtcttc
     2941 ggccctgggc gcaaagcgga ccgccagcag cgaaaccacc gcctcgggcg cgcccagctt
     3001 cagattccgc gatttgaccg acaaaacgcc gtcgaaatgc aggccggcgc gcacgcgcag
     3061 attctcgcgc ttgccgcttt cccatttgaa gcggttgaac agagcgacaa agcgccgctt
     3121 cttcggcaga tagacgagat cgcccagacg cgcgacggcg tcctgcaagc gggcggaaat
     3181 cacctgcaaa tcctcggcgt ccatcgccgc cagaaccgga gcgcgcgtgc tcatgccggc
     3241 agcctttcga tctcggcgcc gacgcggccg agtttttcct ccagccgctc gaagccgcgg
     3301 tccaggtgat agacgcgatt gacggcggtc tcgccttccg ctcccaagcc cgccagcacc
     3361 aagctcgacg aagcgcgcaa atcggtcgcc atcaccggcg ctcccttcag ccgcggcacg
     3421 ccgctcacca cggccgtgtc gccctcgatc ttgatgtcgg cgcccatccg cagaagttcc
     3481 ggcacatgca tgaagcggtt ctcgaaaatc gtttccttga tggtggacgt tccctcggcc
     3541 gtcgccatca gcgccatgaa ctgcgcttgc aggtcggtgg gaaagcccgg aaaaggcgcc
     3601 gtcgcggcgg agacggaaat cggcctgacg ccgttgcgcg ccaccgacag tccgcgattg
     3661 gtctcggtga cctgggcccc cgccgcttgc agcaacgcga cgatcgagcc gatgagatcg
     3721 gcccgcgttc cggtcagctc gacctcgccg ccggcgatcg ccaccgccat ggcgtaggtg
     3781 ccggtctcga tccggtccgg cagcacggaa tgctcggcgg catgcaggct cttgacgcct
     3841 tcgaccacga tggtcgacgt gcccaggccg gaaatccgcg cccccatggc gatcaggcaa
     3901 tgtcccaaat cgtcgatctc cggctcacgg gcggcattgg cgatcgtgct gcggccttcg
     3961 gcgagcgtgg cggccatcag cacatgttcc gtcgccccca cggagacgct gggaaattcg
     4021 ataagcgcgc ccttgaggcc ctgcggcgcg ctggcgatca catagccgtc ggccagctcg
     4081 accaccgcac cgagcgcttc cagcgccttg agatgcagat ccaccgggcg agcgccgatg
     4141 gcacagccgc caggcagcga gaccttggcg cggccggcgc gggccagcag aggacccaga
     4201 accaggaagc tcgcccgcat cttgcgcacg atgtcgtagt cggccgtcgt gtccgtgaga
     4261 tcggcggcgc aaagccgata gcgtccgccc atcgccgctt ccggcgccca ttccgcgtcg
     4321 gcgcccaaat ggcgcagcag gtcggccatg aaggccacat cggccagctt ggggacgttg
     4381 gtcaaggtca ggggcgcatc ggtgagcagg cacgcggcca tcagcggcag ggcggcgttc
     4441 ttggcgccgc tgatcgggat ttcgcctttg aggcgaaccc cgcctctaat cactatttta
     4501 tccatgaccc ctcaaacgag catcggcccg caaacggtgg gcgagcgttt tagcgcgcaa
     4561 gggccgccaa caacagcgct aatccgaggg tcgattatcc cggttttgag ggcccttttg
     4621 gagccgcggc cggcaacttg cggctgtgga ccgcggcttc ggccggcgtc tcggcctcct
     4681 cggcggcccc agcccgcagg cgcgccttgc ggcgcttcag attctcgcgc agcgcggcgg
     4741 caagccgttc ccgccgctcg gcggatttgc ccggttttgc cacgccgcag ccctttccct
     4801 ttcacccgga cctaggctat agcagccctc ctttcgggcc acggcaacac cgcggtccgc
     4861 cggatgccgg agtagcgtag tggtagcgca accccttggt aaggggtagg tcgacagttc
     4921 aattctgtcc tccggcacca gtcttcgctc gcaaagcgag agaagactgc ccggcgtagt
     4981 ccatttgggc gaagcctggg ctgcatcccg cgagcttcgc ctggcacgcc agccctcgcc
     5041 tcatatttgc aagcctgcca ttggggctgc ttgctccgcg ctcttcgccg gaccgcctcc
     5101 accggccatg cgttcctcgg cgatggcgtc ggcgatgacg ctggtcgggc gtttctcggc
     5161 atcggcgcgg cggaaaatgg cggtcagcgt atcggcgatg gcgagcgtgc gccgtttgat
     5221 ctccgcgtcg ctccagccga gaatttgccc cgccacgcga atgatgcccc cgccgttgat
     5281 ggcgtaatcg ggcgcataga ggatgccgcg ctccgtcaac atcgcgccat gctcggcgcg
     5341 cgccagctgg ttgttggccc cgcccgccac cacgcgcgcg tgaagacggg gaatggtctt
     5401 ttcgttcagg atcgcgccca gcgcgttcgg cgacagcacg tcgcattcca ccgtgacgac
     5461 atcgtcgggc gaggcgatgc gcgccctgaa ggcgtccgcg gcgcggcgca ccaggtcttc
     5521 gttgatgtcg gcgacgatga gcttggcctc ttcttcgtgc aggcgcttgg cgactcccat
     5581 gccgaccttg cccaagccct ggatcgcgac cgtcaatccc ttgacc
//
LOCUS       DASWPU010000576         3786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_29143,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000576 DASWPU010000000
VERSION     DASWPU010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29143"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VGC27_12200"
     CDS             complement(<1..449)
                     /locus_tag="VGC27_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0283372.1"
                     /translation="MLPITINPRMVRVGLAGAGDGLARREALLAESGVEAVSVAGESS
                     DAALHCLSVLFIAGLERPAAAELARRARAGGVLVNVEDAPELCDFHVPATVRRGRLLL
                     TVSTGGRSPGLARRLREWLQARFGPQWDGQLEILGAARTGWRADGLPP"
     gene            complement(564..800)
                     /locus_tag="VGC27_12205"
     CDS             complement(564..800)
                     /locus_tag="VGC27_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEY0283373.1"
                     /translation="MLTNLTNVKVEISVVLGRSVIPMHQLLRMGRGAVIELDSRQDDP
                     VTILANDRPVAKGDIIIHGDKIGVSVTDLLKGYI"
     gene            841..1503
                     /gene="lipB"
                     /locus_tag="VGC27_12210"
     CDS             841..1503
                     /gene="lipB"
                     /locus_tag="VGC27_12210"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY0283374.1"
                     /translation="MLSDTLTQAPKDAVEWQVRDGLIPYPEAVAFMEERAGAIAAGRA
                     AELVWLVEHPPIYTAGTSAKDADLLDARFPVFKTGRGGQFTYHGPGQRVGYVMVDLKR
                     RTADVHAYVRDLEEWLIRTLARFGVKGELREGRVGIWVARPGREDKIAALGVRVRHWV
                     TFHGVSLNVAPELSHFSGIVPCGIREYGVTSLADLGLKTSMAEVDAALKEAFREIFRP
                     TT"
     gene            complement(1510..1812)
                     /locus_tag="VGC27_12215"
     CDS             complement(1510..1812)
                     /locus_tag="VGC27_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY0283375.1"
                     /translation="MSDDDDDDDLTSLRLVIEGFVQAVGFRQFVIGEARKLALDGWVR
                     NCANGTVEALVSGPTKAVETFVGLCMRGPSGARVTNVELHRAEPPLEKGFHRGVSF"
     gene            complement(1809..>3786)
                     /locus_tag="VGC27_12220"
     CDS             complement(1809..>3786)
                     /locus_tag="VGC27_12220"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0283376.1"
                     /translation="NRGEIACRIIKTARRMGIVTVAVYSDADRDALHVEMADEAVRIG
                     PAPSALSYLRADAILEAAHVTGAEAIHPGYGFLSERQAFAEAVTKASGLVFIGPSPEA
                     IAAVGDKIQSKKLAQSVNIATIPGFVGEIVDMGHARRIVEDIGYPVIVKSSAGGGGKG
                     LRIVHAENELPQAIHSAHNETRASFGDDRLFIEKFFPDTRHIEIQVVGDKHGSVVSLG
                     ERECSIQRRHQKVIEETPSPLLDAAMRAAMSERAVALAKASGYDSVGTVEFVVDKNRK
                     FYFLEMNARLQVEHTVTEMVTGLDLVELMIRVAAGEKLPFAQSDVKPQGWAVEARVYA
                     EDPQRGFLPSAGRLLHYCTPPQGTRGGTTLRIDTGVQEGTEISVHYDPIIAKICTRAP
                     TRDAAIEALADALDGIAIGGIRHNVAFLSAIMHNQRFREGRLSTAFVAEEFPHGFRGR
                     TPDAAAKRRFLAAGVAAELVRMRRASAISGTLNGPHSPGSSFTATLGGKPFAISGATL
                     REGRLFAQIDGEGYAAATDWRPGMPILHLREKEREYAMQIASAPGGWKVSQGGIQAFV
                     CVRSLKAAELATLMPKKAIADTSKLLLCPMPGLVSTVYVEAGQAVKTGEPLVVVEAMK
                     MENVLTAERGGTIGKINVKKGDNLALNDVILEFA"
BASE COUNT          665 a         1251 c         1183 g          687 t
ORIGIN      
        1 ggcggcaggc cgtcggcgcg ccagccggtg cgcgcggcgc cgaggatttc gagctgtccg
       61 tcccattggg gaccgaagcg tgcttgcagc cattcgcgca gccgtcgcgc caagcccggc
      121 gagcggccgc ccgtcgagac cgtcaacagc aacctgcctc gccggaccgt ggcgggcaca
      181 tgaaagtcgc aaagctcggg cgcatcctcg acattgacca gcaccccgcc cgcgcgcgcc
      241 cgccgcgcta gttcggccgc tgccggccgt tccagaccgg caatgaaaag cacggacagg
      301 caatgcaggg ccgcgtcgct gctctcgccg gccacgctga cggcctccac gccggactcg
      361 gccagcagcg cttcgcgccg cgccagcccg tcgcctgcgc cagctaaacc gaccctgacc
      421 attcggggat tgatggtgat gggaagcacg aaacgacctt tgcccttgcg tagcccgccg
      481 gaactacaaa gaggtacgtg ctatcgatat ataatgcaag tatatggtgg aaaataaatc
      541 aaactacatc ttacaaagtg tgtttatatg taacccttta gaagatcggt gaccgaaacc
      601 ccgattttat cgccatggat gatgatgtcg ccctttgcca ccggcctgtc attggccagg
      661 atggtcaccg gatcgtcctg gcgggaatcg agttcgatca cggcaccgcg gcccatccgc
      721 agaagctggt gcatcggaat caccgaacgg ccgagcacga cggagatttc gacctttacg
      781 ttggtaagat tggtcaacat gggccatatc cagaggtgtc gctcaaatcc ttaggaccac
      841 atgctttcgg acacgttaac gcaggctcct aaagacgccg tcgaatggca ggttcgggac
      901 gggctcatcc cctatcccga agccgtcgcc tttatggagg aacgtgccgg cgccattgcg
      961 gccggacgcg ccgccgaact cgtctggctc gtcgagcatc cgccgatcta caccgcgggc
     1021 accagcgcca aggacgccga tttgctggac gcacgcttcc ccgtcttcaa gaccggccgc
     1081 ggcgggcagt tcacctatca cggccccggc cagcgcgtcg gctatgtcat ggtcgatctc
     1141 aagcggcgga ctgcggacgt gcacgcctat gttcgcgatc tagaggagtg gctcatccgc
     1201 actttggcgc gtttcggcgt gaagggtgag ctacgcgagg gacgcgtcgg catctgggtg
     1261 gcgaggccgg gccgcgagga caagatcgcg gccctgggcg tacgcgtacg gcactgggtg
     1321 accttccacg gcgtcagcct gaacgttgcg ccggagcttt cgcatttttc cggaatcgtg
     1381 ccgtgcggca tccgcgaata cggcgtcacc agccttgccg acctggggct gaaaacctcc
     1441 atggccgaag tcgacgccgc cctcaaggaa gcctttcggg agatcttccg cccgacgact
     1501 tgaacgaccc tagaacgaaa ctccgcggtg gaagcccttc tccagcggcg gttcggcccg
     1561 gtgcagttcg acattggtga cgcgcgcccc tgacggcccg cgcatgcaca gcccgacgaa
     1621 ggtctcgacg gcttttgtcg gccccgacac cagcgcctcg accgttccgt tcgcgcaatt
     1681 gcgcacccag ccgtcgagcg cgagcttgcg cgcctcgccg attacgaatt ggcggaagcc
     1741 gaccgcctgc acgaagcctt cgatcaccag gcgcagagag gtgagatcgt cgtcgtcgtc
     1801 gtcgtcgctc atgcgaactc caatatcaca tcgttgaggg cgaggttatc gccttttttg
     1861 acgttgatct tgccgatcgt gccgccccgt tcggcggtca ggacattctc catcttcatc
     1921 gcctcgacca cgaccagcgg ttcgccggtc ttcaccgcct gccccgcttc cacatacacg
     1981 gtggagacaa gacccggcat cgggcagagc agcagcttgg aggtatccgc tatcgccttc
     2041 ttcggcatca gcgtggcaag ctccgccgcc ttgaggctgc gcacgcagac gaacgcctgg
     2101 atgccgcctt gcgagacctt ccaaccacca ggggcagagg cgatctgcat ggcgtattcc
     2161 cgttccttct cgcgcagatg caggatgggc attccgggcc gccaatcggt cgccgcggcg
     2221 taaccctcgc cgtcgatctg cgcgaaaagc cggccttcgc gcagagtggc gccgctgatc
     2281 gcgaacggct tgccgccgag cgtggcggta aaagacgatc ccggcgagtg cggaccgttg
     2341 agcgtgccgg agatggcgct ggcccgtctc atccgcacca attccgccgc gacaccggcc
     2401 gccaggaagc ggcgcttggc tgccgcgtcc ggcgtgcggc cgcggaatcc gtgcgggaat
     2461 tcctcggcta cgaaggccgt cgacaaccgc ccttcgcgaa agcgctggtt gtgcatgatg
     2521 gcgctcagaa acgcgacatt gtggcggatg ccgccgatcg cgatcccgtc aagcgcatcc
     2581 gccagcgcct cgatcgccgc gtcgcgcgtg ggtgcgcggg tgcagatctt cgcgatgatg
     2641 ggatcataat gaacggagat ttccgtccct tcctgcacac cggtgtcgat gcgcagcgtc
     2701 gtgccgccgc gcgtaccttg cggcggcgta caatagtgca gcagcctgcc tgccgacggc
     2761 agaaagccgc gctgcggatc ctcggcatag acgcgcgcct cgacggccca gccttgcggc
     2821 ttcacgtcgc tttgcgcgaa aggcagcttt tcccctgcgg cgacccgtat catcagctcc
     2881 accaggtcaa ggcccgtgac catctcggtc accgtatgtt cgacctgaag ccgcgcgttc
     2941 atttcgagga aatagaattt gcggttcttg tcgacgacga actccacggt gccgacgctg
     3001 tcgtaaccgg aggcctttgc cagcgccacc gcccgttcgc tcatggcggc gcgcatcgcg
     3061 gcgtcgagca gcggcgacgg cgtctcctcg atcaccttct gatggcggcg ctggatcgag
     3121 cattcgcgtt cgcccaggga gacgacgcta ccgtgcttgt cgcccaccac ctggatttcg
     3181 atatggcgcg tatcggggaa aaatttctcg atgaacaaac ggtcgtcgcc gaagctggcc
     3241 cgggtttcgt tgtgcgccga atggatcgcc tgcggcaatt cgttttcggc atgcacgatg
     3301 cgcaagccct tgccgccgcc gccggccgac gacttcacga tcaccggata gccgatgtcc
     3361 tcgacgatgc gccgggcatg ccccatatcc acgatctcgc cgacgaagcc ggggatggtg
     3421 gctatgttca ccgactgcgc cagctttttg gattggatct tgtcgccgac ggccgcgatg
     3481 gcttccgggc ttgggccgat aaagaccagg ccgctggcct tggtcaccgc ctcggcgaac
     3541 gcctggcgct ccgataggaa gccatagccg ggatggatgg cctcggctcc cgtgacgtgt
     3601 gcggcttcga ggatcgcatc ggcgcgcaga taggacagag cggagggggc gggaccgatg
     3661 cgaacggcct cgtcggccat ttcgacatgc agggcgtctc gatccgcgtc cgaatagacc
     3721 gcgacggtga caatgcccat ccggcgggca gtcttgatga ttcggcaggc gatttcgccg
     3781 cgattg
//
LOCUS       DASWPU010000577         5926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_121718,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000577 DASWPU010000000
VERSION     DASWPU010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121718"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..204)
                     /locus_tag="VGC27_12225"
     tRNA            complement(130..204)
                     /locus_tag="VGC27_12225"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Gly,seq:gcc)
     gene            complement(422..3322)
                     /locus_tag="VGC27_12230"
     CDS             complement(422..3322)
                     /locus_tag="VGC27_12230"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEY0283377.1"
                     /translation="MTALSISVSALPKPFDRARAGRTFERLAEEGYRPQGNARALLES
                     AFGNSAFLCRLALREHEMLARLFAEGPHALLHEAITGALAAADAEKEAEIMAALRQAK
                     RRAALAVALADISGAWRLEEVTQALTEFADACVKGALRFALREVAQGTPLAGRNGAAL
                     EASTGLLVLAMGKYGAFELNYSSDIDIIVFYDADTFPFRKRGDARGAAVDIVKKLVKL
                     IGEVTGEGYVFRVDLRLRPDAGATQIAISSDAAISYYEEMGQNWERAALIKARACAGD
                     PAAGARFLAAIEPYVWRRNLDFAAIEDIQSIKRQIHAHVGHGEIAVAGHNIKLGRGGI
                     REIEFFAQTQQLILGGRDPALRQRGTIAALEALRDRGHASAAAARELSKAYRFLRTLE
                     HRLQMMEDEQTHTVPKTPQGVAHTACFMGFADTPAFALALLDELETVQGHYAHLFERE
                     APLASAGGNLVFTGVEDDPETLKTLARMGFRDPAHVSGAIRGWHHGRIRAMRSTRARE
                     LLTKLVPALLAALAATADPDAAFAQFDRFLSNLPAGVQLFSLLLAHSELLRLIAEIMG
                     SAPRLAAHLARSPATLDALLDTDFLGTLPSRAALDGALQSQLKRAGTYEAVLDAVRRF
                     AKEQGFRVGVQVIEGAAKADAAGPAFAAIAESVVVNLLPHVAEALVEGAGRVPGGQFA
                     VIAMGKLGGREMTAGSDLDLVFVYDAPTKVEQTDGRKPMPMSFYYGKLAQRLIAALTV
                     ATAEGGLYEVDMQLRPTGSKGPVAVSLESFRRYHQTESWTWERMALTRARVIAGPREL
                     ATKLDAVVSATLCRRSDPAKLFADAREMRAKVAAAYPGKNPWDLKYAPGGLMDIEFAA
                     QILQLRHAHENPSLLSTNTVAALKLLAKAGALGEIDAEALIAAAKLEHALTQALRIAL
                     DGPLDAAAATPGLKALLARAGEVSDFGVLQKLLADLQARARQISERILHIGK"
     gene            complement(3477..4919)
                     /locus_tag="VGC27_12235"
     CDS             complement(3477..4919)
                     /locus_tag="VGC27_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0283378.1"
                     /translation="MRGSLFRHWAPGILRTQAFRIVLIYVFVFAVSATLLVGFTYWNT
                     ERSLDRQTDQIILAEITGLVEQYQRLGAMGLAEAVARRSAREGQSLYILADDRGRWLA
                     GNLDQWPPSITRANEFVEFGYDRRVQGQLTTRKARGMALLLVGGFELLIARDVAERDL
                     TRRLLTTTLPWSVGLMLLFGLIGGALMSRNVLRRLDSINRTSAEIAAGDFSRRVPLTR
                     AHDEFDMLAENLNRLFERIERLIKGVREVADSVAHDLRTPLNRLRNRLEETQRRLDPQ
                     SPHAAEIEAAIAETDGLIATFNALLLIAEADSGVARGAMSQNDLSAVVADVAELYAPL
                     AEEKNIALTVVPTGILTLECNRSLISQALANLIDNAIKYTPAGGRVTISAAETPSGVD
                     LTVADSGPGIPAEDRSRAVERFVRLEASRNSPGTGLGLSLVAAVARLHQARFMLDDNN
                     PGLKATISLPRSPPRPNKGANRESRTGSSE"
     gene            complement(4909..5583)
                     /locus_tag="VGC27_12240"
     CDS             complement(4909..5583)
                     /locus_tag="VGC27_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0283379.1"
                     /translation="MRILVVEDDLEAQRYLVNGLKEAGHVVDDAADGETGLTLALSRP
                     YDVAIVDRMLPKMDGLNVVAEMREHGNNMPILFLSALSEVDDRVKGLKAGGDDYLTKP
                     YAFVELLARIDALMRRHAPASVKTKLAVGDLELDLLTRAATRAGQAIDLQPREFRLLE
                     YLMRHAGQVVTRTMLLESVWEYHFDPQTNVIDVHISRLRAKIDKGFDAALLHTVRGAG
                     YMIRAR"
     gene            complement(5712..>5926)
                     /locus_tag="VGC27_12245"
     CDS             complement(5712..>5926)
                     /locus_tag="VGC27_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0283380.1"
                     /translation="ITKVDPNSDAAEKGLQPGDVLLKVNNRDVHTPQDVEKSVAAAKS
                     AGRKSVLLLVSSDGTSVFVAVDIDKT"
BASE COUNT          998 a         1948 c         1996 g          984 t
ORIGIN      
        1 gtccgggaag cccgcggcgg gtctggatca ggggtggcgg agagggaaag acgacgaacg
       61 tcacgtcgaa gcgcaatgga agcaattcgc ttccaggcgc caagacataa cgattcgatc
      121 ttgggaaatt ggagcgggcg aagggattcg aaccctcgac cctaaccttg gcaaggttat
      181 gctctacccc tgagctacgc ccgcattcca agtgggacgc gggttatcgc ctagcccgca
      241 ccccaattca agccggttct gacgatccgg gccggcagcg gccttttggc aagcgggtgg
      301 ggcggatttg cctgtctgcg ccaactcccg cgccaccgcc cacaccaact cccgccccga
      361 ccacgccgtc cgccaactcg tgcagcctgc cggcgccacc tcctcttgtg cccggccggg
      421 gttacttccc gatatggaga atgcgttcgg agatctgccg cgcccgggcc tgaaggtcgg
      481 cgagcagctt ttgcagcacc ccgaaatccg acacctcgcc cgcccgcgcc agcagcgcct
      541 tgagaccggg agtggccgcc gccgcatcga gcggcccgtc gagcgcgatg cgcagcgcct
      601 gggtcagcgc gtgctcgagc ttggccgcgg cgatcagcgc ctcggcgtcg atctcgccga
      661 gcgcgcccgc tttggccaaa agtttgagcg ccgcgacggt gttggtggac agcagcgaag
      721 gattctcgtg ggcgtggcgc agttgcagga tctgggcggc gaactcgatg tccatcaagc
      781 cgccgggtgc atatttcaag tcccagggat ttttccccgg atacgccgcc gccacctttg
      841 cgcgcatttc ccgggcgtcg gcgaagagct ttgcggggtc cgaccgccgg cacagcgtgg
      901 cggagacgac ggcatcgagc ttggtggcga gttcgcgcgg gccggcgatc accctcgccc
      961 gcgtcagcgc catgcgctcc caggtccagg attcggtttg gtgatagcgc ctaaaggatt
     1021 ccaggctcac cgccaccgga cctttgctgc cggtcgggcg cagttgcatg tccacttcgt
     1081 aaagaccgcc ttccgccgtc gccacggtca gcgccgcgat cagccgctgg gccagcttgc
     1141 cgtaatagaa actcataggc atcggctttc tgccgtcggt ctgttcgacc ttcgtcggcg
     1201 cgtcatagac gaagacgaga tcgagatcgc tgcccgccgt catctcgcgg ccgccgagct
     1261 tgcccatggc gatcaccgcg aactggccgc ccggcacgcg ccccgcaccc tcgaccagcg
     1321 cctcggcgac atgcggcagc aggttgacga cgacgctctc ggcaatggcg gcgaaggcag
     1381 ggccggccgc atcggccttg gccgcgcctt cgatcacctg caccccgacg cggaagcctt
     1441 gttccttggc gaagcggcgc acggcatcga gcaccgcttc gtaagtgccg gcgcgcttaa
     1501 gctggctttg cagggcgccg tcgagcgccg cgcgcgacgg cagcgtgccg aggaaatcgg
     1561 tgtcgagcag cgcgtccagc gtcgccggcg agcgggcgag atgcgcggcc agccgcggcg
     1621 ccgaacccat gatctccgcg atcaggcgca gcagctcgga atgggcaagc agcagcgaga
     1681 aaagctgcac cccagccggc agattgctga ggaagcggtc gaactgggcg aaggcggcat
     1741 cgggatcggc ggtcgccgcc agcgccgcga gcagcgccgg aaccagcttg gtcagaagct
     1801 cgcgggcgcg cgtgctgcgc atggcgcgga tgcggccgtg atgccagccg cggatcgcgc
     1861 ccgagacatg ggcgggatcg cgaaagccca tgcgcgccag cgtcttcaag gtttcgggat
     1921 cgtcttccac cccggtgaag accagattgc cgcccgccga agccagcggc gcctcgcgtt
     1981 cgaacaaatg cgcgtaatgg ccctggacgg tttccagttc gtcgagcagc gccagcgcga
     2041 aggcgggtgt atcggcaaag cccatgaaac aggcggtatg ggccacccct tgcggagttt
     2101 tcgggacggt atgcgtctgc tcgtcttcca tcatctgcag gcgatgttcg agagtgcgca
     2161 ggaagcgata ggccttggac aattcgcgcg cggcggcggc gctggcatgg ccgcggtcgc
     2221 gcaacgcctc caaggcggcg atggtgccgc gttggcgcag cgccggatcg cgtccgccca
     2281 ggatcagttg ctgggtctgg gcgaagaact cgatttcgcg gatgccgccg cgccctaact
     2341 tgatgttatg accggcgact gcgatctcgc catggccgac atgggcgtgg atctgccgct
     2401 tgatggattg gatgtcttcg atggcggcga agtcgagatt gcgccgccag acatagggct
     2461 cgatggcggc gagaaaccgc gcccccgccg ccgggtcgcc ggcacaggcg cgcgccttga
     2521 tcagcgccgc gcgctcccag ttctgcccca tctcttcgta ataggatatg gccgcgtcgc
     2581 tggagatggc gatctgagtg gcgccggcat cgggacgcag gcgcagatcg acgcggaaga
     2641 catagccttc gccggtgact tccccgatca gcttgaccag cttcttgacg atatccacgg
     2701 ccgcgccgcg cgcgtcgccg cgcttgcgga agggaaaggt gtccgcgtcg tagaagacga
     2761 tgatgtcgat gtcgctggag tagttcagct cgaaagcgcc gtatttgccc atggccagaa
     2821 ccagcagacc ggtagaggcc tccagcgccg cgccgttacg cccggccagc ggcgtgccct
     2881 gcgcgacctc ccgcaaagcg aagcgcagcg cgcccttgac acaagcgtcg gcgaattcgg
     2941 tgagcgcttg cgtgacctct tccagcctcc aggcgcccga aatgtcggcc agcgccacgg
     3001 cgagcgcggc gcggcgcttg gcctggcgca gcgccgccat gatctccgct tccttctccg
     3061 cgtccgcagc ggccaaggcg ccggttatag cttcgtgcag aagcgcgtgc ggaccttcgg
     3121 cgaacagacg cgccagcatt tcatgttcgc gcagcgccag ccggcagaga aaggcgctgt
     3181 tgccgaaagc ggattccagc agggcgcgcg cgttgccttg cgggcggtag ccctcctccg
     3241 ccagacgctc gaaggtgcgc ccggcgcggg cgcggtcgaa cggcttgggc agagcggaga
     3301 cggaaataga gagggcggtc atggggaaaa gatacagcga atggcgaatg gcgaatagcg
     3361 aatagggaat agcgaacaat gcccccttcg tccgtcttcg ccaaggcttc ggcggacact
     3421 tcccccgcaa cccgccttcg ctaaagctac ggcgggcatg gcgggggcag aaatccctat
     3481 tcgctactcc ctgttcgcga ttccctattc gcccccttgt tcgggcgcgg gggagagcgc
     3541 gggagcgaga tcgtggcttt caggccgggg ttgttgtcgt ccagcatgaa gcgcgcctgg
     3601 tgcaggcgcg cgaccgccgc caccagactg aggccgaggc ccgtgcccgg cgaattgcgg
     3661 ctcgcttcca gccgcacgaa acgctcgacg gcgcggctgc gatcctccgc cggaataccc
     3721 ggcccgctat cggccaccgt cagatcgacg ccgctcggcg tttcggcggc ggaaatcgtc
     3781 acccgccctc cggccggtgt gtatttgatc gcattgtcga tcaggttggc gagcgcctgg
     3841 ctgatcagac tgcggttgca ttcgagcgtc agaatgccgg tgggcacgac ggtcaaggcg
     3901 atgttcttct cttcggccag cggcgcgtaa agctcggcga cgtccgccac caccgcggaa
     3961 agatcgttct ggctcatcgc cccgcgcgcc acgccggaat cggcttcggc gatcaagagc
     4021 agggcgttga aggtggcgat cagtccgtcg gtctcggcga tggccgcttc gatttcggcg
     4081 gcgtgcgggc tttgcggatc gagccggcgc tgcgtttctt ccagccggtt gcgcaggcgg
     4141 ttgagcgggg tgcgcagatc gtgcgcgacg gaatcggcca cctcgcgcac gccctttatc
     4201 aggcgctcga tgcgttcgaa cagccggttg agattctccg ccagcatgtc gaattcgtcg
     4261 tgcgcccggg tcagcggcac gcggcgcgaa aaatcccccg ccgcgatctc ggcgctggtg
     4321 cggttgatcg aatccaggcg ccgcagcacg ttgcggctca tcagcgcccc gccgatcaga
     4381 ccgaacagca gcatcagccc gacgctccag ggcagcgtcg tggtgagaag ccgccgggtc
     4441 agatcgcgtt cggcgacgtc gcgggcgatc aagagttcga agccgcccac cagcaacaag
     4501 gccatgccgc gggcttttct ggtcgtcaac tggccttgca cgcggcgatc gtagccgaac
     4561 tcgacaaact cattggcgcg cgtgatcgac ggcggccatt ggtcgagatt gccggcgagc
     4621 cagcggcccc ggtcgtcggc cagaatgtag aggctttgcc cttcgcgcgc cgaacggcgg
     4681 gcgaccgctt cggccagccc catggcgccc aggcgctgat attgttcgac caggccggtg
     4741 atctcggcga ggatgatctg gtcggtttgg cggtcgagcg agcgctcggt gttccaatag
     4801 gtgaagccga ccagcagcgt cgccgacacg gcgaagacga agacgtagat cagaacgatc
     4861 cggaacgcct gggtgcgcag gatcccgggc gcccagtggc ggaaaaggct accgcgcacg
     4921 gatcatgtag cccgcgccgc ggaccgtgtg gagcaacgcg gcatcgaagc ccttgtcgat
     4981 cttcgcccgc agccgcgaga tgtgcacgtc gatcacgttg gtctgcggat cgaagtgata
     5041 ttcccagacg ctctccagca gcatggtgcg ggtgacgacc tgcccggcat gacgcatcag
     5101 atattcgagc aggcggaact cgcgcggctg gaggtctatg gcctggccgg cgcgcgtcgc
     5161 cgcgcgggtc agaaggtcca gttccagatc gccgaccgcg agcttggtct tcaccgaagc
     5221 gggggcgtgc cggcgcatca gcgcgtcgat ccgcgcgagc aactcgacga aggcgtaggg
     5281 cttggtgaga tagtcgtcgc cgcccgcctt cagccctttc acccggtcgt ccacctcgga
     5341 caaggcgctg aggaaaagga tcggcatgtt gttgccgtgc tcgcgcatct cggccacgac
     5401 attgaggccg tccatcttcg gcagcatgcg atcgacgatg gcgacatcat aaggccgcga
     5461 caaggccagg gtcagcccgg tttctccgtc ggcggcgtcg tcgacgacgt ggcccgcctc
     5521 cttgagtccg ttgacaagat aacgttgcgc ttcgagatcg tcttccacga cgagaatgcg
     5581 catgcccgcc tccgcttgag gatataaggg ccgcgccgac ggcagcaacg ggggggatgg
     5641 ggccgccgcc gccggcgcgg gggccgggcg ccgccccgct gtcgcgaagc gaagcccggt
     5701 acgcaacaca ctcaggtctt gtcgatgtcc accgcgacga aaacagaggt tccgtcactg
     5761 gataccagca agagcacgct tttgcgcccg gcggacttcg ccgcagcaac actcttctcg
     5821 acatcctgcg gcgtatgcac gtctctgtta ttgaccttga gcagcacatc gcccggctgc
     5881 aggcccttct cggccgcatc gctgttggga tcgaccttgg tgatca
//
LOCUS       DASWPU010000578         3833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_84919,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000578 DASWPU010000000
VERSION     DASWPU010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84919"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /gene="mutL"
                     /locus_tag="VGC27_12250"
     CDS             complement(<1..1373)
                     /gene="mutL"
                     /locus_tag="VGC27_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY0283381.1"
                     /translation="MKIRRLSEGTVNRIAAGEVVERPASAVKELVENALDAGAAHIDI
                     AASNGGCDLLLVEDDGEGMEADDLILAVERHATSKLGGDDLSHILTLGFRGEALPSIG
                     AVAHLSIVSRTKKSEAHEVRVEGGRAQGPMPAGFRAKGQSGTRVEVRELFYATPARLK
                     FLKSARSEDIATQDIVKRLAMARADVAFTLTLDGRRALDLQAEADLFSGRLNRLAKIM
                     GRDFGDNAAPVSADREGVRIAGFAGLPTYNRANSMQQFLFVNGRPVRDKLLIGAVRGA
                     YADFLARDRYPALALFLDCDPAFVDVNVHPVKTEVRFRDAGLVRGLIVSALKLALHEA
                     GHKASTTVAGDALAALRPHSGYAESDFRPSPYRPSSAAYDTARDFQSPLFTGGAPSAR
                     VEAAPEAPSTDNPLGAARAQLHETYIVAQTADGIVIVDQQAAHERLTYEKMKHAMEQG
                     GIARQPLL"
     gene            complement(1557..2879)
                     /locus_tag="VGC27_12255"
     CDS             complement(1557..2879)
                     /locus_tag="VGC27_12255"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0283382.1"
                     /translation="MTPRRAIAVLLFLVAALAPASAANVKTLNAVKGAQVWFAEDHTV
                     PMIALTASLPAGSAYDPSAKAGLAAFAAYLLNEGAGNLDSTAFQAALADRAIQLGVSP
                     GRDSLTVSLAVLSSHAKEAFRLLGLALSRPRFDADAVSRVRAQMLQGLEEAKGDPGSV
                     AEDSFYSFYFGAHAYGHPPEGAARALMATTPEDLKTFARTHWVRGGLRIAVVGDVNAA
                     TATALLHSAFGALPEAAPPLPPVPMRVGAPGLHVVPMDVPQPVALFALPGFVRNDPDF
                     LTGYIANYILGGGGFASRLTKEVRGKRGLTYDISTDLIPYRRAGVLLGQVAARKDAMR
                     QTIAVVREVMRKFAAEGPTPQELAEAKTYLNGSFPLTFASNMGLSAQLNAFQQMGLGI
                     DYLNKRAGLINAITLGDVRRIARRLCNPAKMTIVVAGSLPEEKTRLVQ"
     gene            complement(2876..>3833)
                     /locus_tag="VGC27_12260"
     CDS             complement(2876..>3833)
                     /locus_tag="VGC27_12260"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0283383.1"
                     /translation="LEADRMANLDLSESAVGTERDVVLAERRMRIDNDPEALTQEQAE
                     AALYLSHPYGRPVIGWADEIRHIGRVEAGDFYRRHYAPNNAILVVSGDVTVDEVRAAA
                     EAEYGVVSSHELAVRVEYAQPPRLGATRLVVARKDAKVPLFLRFYRVKSYAEAAPGEA
                     EALEVLARLLGGDATSALYRRLVVERRLATDVSAYYGGYTRDAGEFGFVARPRPGVSL
                     ETLERAIDEVVAPYLRRAPSKAELTRAKTQLVAGVTFRRDSQFEMASAYGRALSIGLT
                     MYDVQQWPGRIQAVTREDVRETASASLVRKESVTAYLLPGSQ"
BASE COUNT          671 a         1259 c         1302 g          601 t
ORIGIN      
        1 agcaagggct ggcgggcgat gccgccctgt tccatcgcat gtttcatctt ctcgtaggtc
       61 aggcgctcgt gcgcggcctg ctgatcgacg atgacgatgc cgtccgcggt ctgtgccacg
      121 atgtaggttt cgtgcaactg cgcccgcgcg gcgccgagcg ggttatccgt tgacggagct
      181 tctggggcgg cttcgacgcg ggcggatggt gcgccgccgg tgaataaagg cgattggaaa
      241 tcacgcgcgg tgtcgtaagc ggcggaagac gggcgataag gcgacggacg gaaatccgac
      301 tcggcatagc ccgaatgcgg gcgcaaggcg gcgagcgcat cacccgcgac ggtggtggac
      361 gccttgtgcc cggcttcgtg cagcgcaagt ttgagcgcgg agacgatcag cccgcgcacc
      421 agcccggcgt cgcgaaagcg gacttcggtt ttcaccggat gcacgttgac gtccacgaac
      481 gccggatcgc aatcgaggaa cagcgccagt gccggatagc ggtcgcgcgc caggaaatcg
      541 gcataggcgc cgcgcactgc gccaatgagc agcttgtcgc gcaccggccg cccgttgacg
      601 aacaggaatt gctgcatcga attggcgcgg ttgtaggtgg gcagccccgc gaagcccgcg
      661 atcctgacac cttcgcggtc cgcagaaacc ggcgcagcat tgtcaccgaa atcgcggccc
      721 atgattttcg caagcctgtt cagccgtccg ctgaacaaat cggcctcggc ttgaagatcg
      781 agcgcgcgac gtccatcgag cgtcagcgtg aaggcgacat cggcgcgcgc catcgccagg
      841 cgcttgacga tatcctgggt ggcgatatct tccgagcgcg ccgatttgag gaacttaagc
      901 cgcgcgggcg tggcgtagaa cagctcgcgc acttcgacgc gcgtgccgga ctgacccttg
      961 gcgcggaagc ccgccggcat cggcccctgg gcgcggccgc cctcgacgcg gacctcgtgc
     1021 gcctcgctct tcttcgtgcg gctgacgatg gacaggtgcg ccaccgcgcc gatggaaggc
     1081 agcgcctcgc cgcggaagcc gagcgtcaga atgtgcgaaa ggtcgtcgcc gccgagcttg
     1141 gaagtggcat ggcgttccac cgcgaggatg agatcgtcgg cctccatgcc ctcgccgtca
     1201 tcctccacca ggagcagatc gcagccgccg ttcgaggcgg cgatgtcgat atgcgcggca
     1261 cccgcgtcga gcgcgttctc caccagctcc ttcacggcgc tggccggacg ttcgaccacc
     1321 tcgcccgcgg caatgcggtt gacggtacct tcggagagac ggcggatctt catggaggga
     1381 gtgtagcaga cgtagtttgg tacagtgcag ccccaatgtg tcatggcccg cccagtgcgg
     1441 gccacccagt tggggacaag tgctttgtcg aaagatagag caacggcctc ggcgcaaccg
     1501 gcaagatcga gcgcgcatca cctgggtagc ccgcattcgc gggccatgac agtgagttat
     1561 tggacaagcc gtgtcttctc ttccggcaaa gaccccgcga cgacgatggt catcttcgcg
     1621 ggattgcaga ggcggcgggc gatgcggcgg acatcgccga gagtgatcgc attgatcagg
     1681 cccgcgcgct tgttgagata atcgatgccc aggcccatct gctggaaagc attgagctgg
     1741 gcggaaagcc ccatattcga ggcgaaagtc agcggaaacg aaccgttcag gtaggtcttg
     1801 gcttcggcca gttcctgtgg cgtcggtccc tcggcggcga atttgcgcat cacctcgcgc
     1861 accaccgcga tggtctggcg catggcatcc ttgcgcgccg cgacctggcc cagcagaacg
     1921 cccgcccggc gataggggat caagtccgtc gagatgtcat aggtgaggcc gcgcttgccg
     1981 cggacttcct tggtcaggcg cgaagcaaag ccgcccccgc ccaggatata gttggcgata
     2041 tagcccgtca ggaaatccgg gtcgttgcgc acgaagcccg gcagggcaaa aagcgccacc
     2101 ggctgcggca catccatcgg cacgacatgc agtcccggcg cgcccacacg catgggcacc
     2161 ggcggaagcg gcggcgcggc ctccggcagc gcgccaaagg ccgaatgcag aagagcggtc
     2221 gccgtcgccg cgttcacgtc gccgacgacg gcaatccgca gaccgccgcg aacccaatgc
     2281 gtgcgggcga aggtcttgag gtcttccggt gtcgtcgcca tgagggcgcg cgcggcgcct
     2341 tcgggcggat ggccgtaagc gtgggcgccg aaataaaaac tgtagaagct gtcctcggca
     2401 accgatccag gatcgccctt tgcctcttca aggccttgca gcatctgggc gcgcacgcgg
     2461 ctcaccgcat cggcgtcgaa gcgcggccgg gacagcgcca ggccgagcag ccggaacgct
     2521 tccttggcgt gggacgacag aacggccagc gacacggtga gagagtcgcg gccgggcgag
     2581 acgccgagct ggatcgcgcg atcggccagc gccgcctgga acgcagtgct gtcgagattg
     2641 cccgcgccct cgttcagcag ataggcggcg aaggcggcca agcccgcttt ggcggacggg
     2701 tcgtaagccg atcccgccgg cagcgaggcc gtcagcgcga tcatcgggac ggtgtggtcc
     2761 tcggcaaacc acacttgtgc gcccttgacg gcgttcagcg tcttgacgtt cgccgccgaa
     2821 gcgggcgcca gcgccgcaac gaggaaaaga agaacggcaa tcgcccggcg cggggtcatt
     2881 gcgagcccgg cagcagatag gccgtcaccg attccttcct gaccaagctc gcggacgcgg
     2941 tttcgcgcac gtcttcccgc gtcaccgcct ggatgcgccc cggccattgc tgcacgtcgt
     3001 acatggtcaa gccgatggac agcgcccggc cgtaagccga ggccatctcg aactgactgt
     3061 cgcggcgaaa ggtgacgccg gcgacgagct gcgtcttggc gcgcgtcagt tccgccttgg
     3121 acggcgcccg gcgcaggtag ggcgcgacca cttcgtcgat ggcgcgttcc aacgtttcca
     3181 acgacacgcc ggggcgcggc cgcgcgacga agccgaactc gccggcatcg cgggtatagc
     3241 cgccgtaata ggcgctgacg tcggtggcga gcctgcgctc caccaccagc cggcggtaga
     3301 gcgccgaggt ggcgtcgccg cccaacagcc tggccaggac ttccagcgcc tcggcctctc
     3361 ccggcgccgc ttccgcatag ctcttgacgc gataaaagcg caggaacaac ggcactttcg
     3421 cgtccttgcg ggcgacgacg aggcgcgtgg cgcccaagcg cggcggctgc gcgtattcga
     3481 cgcgcaccgc aagctcgtgc gaggacacca cgccgtattc cgcttcggcg gcggcgcgca
     3541 cctcatcgac ggtcacgtcg ccggacacga ccaggatggc gttgttgggt gcgtaatggc
     3601 ggcggtagaa atcgccggcc tcgacgcggc cgatatggcg gatctcgtcc gcccagccga
     3661 tcaccggccg cccataggga tgggacagat acagcgccgc ctcggcctgt tcctgcgtca
     3721 atgcttcggg atcgttgtcg atgcgcatgc gccgctccgc cagcaccacg tcgcgctcgg
     3781 tcccgacggc cgattccgaa agatcgagat tggccatgcg gtccgcttcc aga
//
LOCUS       DASWPU010000579         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_78256,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000579 DASWPU010000000
VERSION     DASWPU010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78256"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="VGC27_12265"
     CDS             <1..1607
                     /locus_tag="VGC27_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283384.1"
                     /translation="TRLGAVSDASATTLTGATYAANSLDLGALTLTNDTTLDTSAANG
                     SIILASADGTTAGGQGLTIKSGTGKVSLGNLGATTRLGAVSDASATTLTGATYAANSL
                     DFGALTLTNDTILDTSAANGSIVLASADGTTAGGQGLTLKAGTGKISLGNLGATTRLG
                     AVSDASATTLTGATYAANSLHFNGNLTLTAAVTTFDTSAAAGDIVVAGDIFGTANGIQ
                     SLVLIAGPGTGSAAANGNISLQNAGTQTTRLGNMSANGNNFSALTVYLAGSFGSHLAG
                     NQVFSADTLDTLGSVNSTVAGNASGHIAAGGAVTFGVGGNLSGTISGGAISLTTGSIS
                     NATITGTGNLIVVSPSVTGSTFTAPSVNISGGSFNGIVNAASNANIQADTISGTFTGG
                     VITLVATNAVNATITADTLNMRAPHGNVNGTWNTLVTTGSGTLVINGESNLGNGGINP
                     SQLVVEGFVLPLGAQISPSGEIVLPQGLVLGLLSPSGESKPRMILVHDVWRLGQLLEA
                     GYVAIVIDLSGRKKDKDDETLVVASN"
BASE COUNT          315 a          515 c          512 g          271 t
ORIGIN      
        1 cgacgcggct cggggcggtc tccgacgcca gcgcaacgac gctgacaggc gcgacctatg
       61 ccgccaacag cctggacctc ggcgctctga cgctgaccaa cgacaccacc ctcgacacca
      121 gcgcggccaa cggctcgatc atactggcct ctgccgacgg caccacggcg ggtggtcagg
      181 ggctgacgat caagtcgggc accggcaaag tatcgttggg caatctgggc gccacgacgc
      241 ggctgggggc ggtctccgac gccagtgcga cgacgctgac aggcgcgacc tatgccgcca
      301 atagcctgga ctttggcgct ctgaccctga ccaacgacac gatcctcgac accagcgcgg
      361 ccaatggctc gatcgtactg gcctcagccg acggcacgac ggcgggcggc caggggctga
      421 cgctgaaggc cggcaccggc aagatatcgc tgggcaatct gggcgctacg acgcggctcg
      481 gggcggtctc cgacgccagc gcaacgacgc tgacgggcgc gacctatgcc gccaacagcc
      541 tgcacttcaa tggcaacctc acgcttaccg ccgccgtcac cacattcgat acctcggcgg
      601 ccgcgggcga tattgtcgtc gccggcgata tcttcggcac ggcgaacggc attcagagtc
      661 ttgtccttat cgccggtccg gggacgggat cggctgcggc caacggtaac atctcgctgc
      721 aaaatgccgg aactcagact acccgcctcg gcaatatgag cgcgaacggc aataacttct
      781 ccgcgctcac ggtgtatctg gcgggcagct ttggttccca tctcgcgggc aatcaggtct
      841 tctccgccga cacattggac acgctcggca gcgtgaacag cacggtggcc gggaacgcat
      901 ccggtcacat tgctgcgggc ggcgcggtga cgttcggcgt gggcggaaat ctttccggaa
      961 cgatcagcgg cggcgcaatc tctctgacga cgggcagcat ctcgaatgcc acgatcacgg
     1021 gcaccggaaa cctcattgtt gtgtcgcctt ccgtaacggg ttcgacgttt accgcgccga
     1081 gcgtgaatat cagcggaggg tcgtttaacg ggatagtcaa cgctgccagc aacgccaaca
     1141 tccaggcgga cacgatcagc ggtacgttta ccggcggcgt gatcaccttg gtggccacca
     1201 acgccgtgaa cgcgaccatt acggcggaca cgctcaatat gagggcgccg catggcaatg
     1261 tgaacgggac atggaacacc ctcgtcacga caggcagcgg cacgctcgta atcaacggcg
     1321 agtccaatct cggcaatggc gggatcaacc ccagccagtt ggtggtcgaa ggatttgtcc
     1381 tgccgttggg agcgcagata agcccatccg gcgagatcgt attgccgcag ggcttggtcc
     1441 tcgggctgtt gtcccctagc ggcgaaagca agccgcggat gatcctcgtt cacgacgtct
     1501 ggcgcctcgg gcaattgctg gaggccggtt acgttgccat cgtcatagac ctgtcgggcc
     1561 gcaagaagga taaagacgac gagacgttgg tggtggcttc gaactaggtg ttt
//
LOCUS       DASWPU010000580         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_47992,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000580 DASWPU010000000
VERSION     DASWPU010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47992"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3691)
                     /locus_tag="VGC27_12270"
     CDS             complement(<1..>3691)
                     /locus_tag="VGC27_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cadherin domain-containing protein"
                     /protein_id="HEY0283385.1"
                     /translation="FRDFRYELIGGDTDSFTINQLTGEITTLANLDYETRSNYSIQAR
                     VWDGGSLGAGLYGAATINIDVTDVNEAPTNLVFSNTTTSIAENTPTPTRTKVADLSVE
                     DDALGTNVYGLTGPDASAFEIADSALYLKAGVVLNFEAQQSYTVAVTVDDPTLGTGPE
                     LTRSFTLNVTDVNEAPTSLSLTPLVADVSERDRPDPNGPTPPAIKLGVLSASDPDLPT
                     SGDFAQLAYSVSDPRFEILNGNELWLKTGAMSSIDYEAGTTLSVDVTVKDRNGAPEGL
                     ALTRTFTFAVSDQTDVLEGGPGADVLVGQQGRDILRGMGGDDTLQGADGNDDLLGGSG
                     NDTLYGDAGNDYLQADDGNDQLFGGDGADTLLGGDGNDMLYGGAGNDMLDGGAGDDVL
                     DGGPGADIIQGGTGTDTLSYAGSTAGVNVNLATGLGTGGDAEGDTISGIERLIGSSYA
                     DTLTGTAAAEYIDGGAGNDIISGGGGADELHGGDGDDTITGGNDGGWLYGEAGNDILI
                     GGEGVDHLYGGAGNDILRAMGAGDELVGGAGDDILEGGEGSDSYVLDTTSGHDTIRDF
                     DAEGDDHDQINYNGVDTSWLWFERIGNDLKITIVGSSSTATVEGWYADDPPPANRRVE
                     MVLTSTHYSIDVDGLTALMATKAKPADLAALGALMTDSTYASQWAAFWGINQAPVLGA
                     ISAQSTPEDNSLAIAITATDDFTSKDLLHYELRTYSDAGLTQENTATAVQSWNVALNG
                     SSQVLNIAPSANYGGTVYAQLKVFDLTGRMDQKVFTLTVTPVADMPSLTTPAALSGTL
                     DGGSLPLSISADLLDTDGSESLQIWVLGLPGGLSLNKGTNLGGGTWSLTKADLQGLAI
                     TGPANWATDFTLTVRAISTEAINGSTATTPDHSINVTINARPSGISGSLSVNENAANG
                     TVVGMLTGQDPDGDTLTYSLTDTAGGRFAINSVTGAVTVANGSLLDYESASSAAITAR
                     VTDTSGLTKDVPFTVSIIDVNEAPSLSGGTFSIAENLAAGTGVTTLTASDPDATAAYR
                     DFRYELIGGDTGAFAINQTTGAITTLASFNYEARQSYSVQARVWDAGAIGSGNSAVAT
                     VNINVTDVNEAPSLSNQTLSVQEYPVSNPTQTTINLVNPATDPDTLTPGYRNLYYRAI
                     GGDTNVFSMAPNGVITVQQTLDYETRTSYSLQVQVWDGGAYGVGLSSTATVNINVTNV
                     NEAPYIIGVGTSIEYEEWTPENAYIA"
BASE COUNT          676 a         1136 c         1106 g          773 t
ORIGIN      
        1 gcaatgtacg cattttcggg tgtccactct tcgtattcta tggaggtgcc cacaccgatg
       61 atatacggtg cttcattgac attcgtcaca ttgatattaa cggtcgccgt agaggaaaga
      121 ccgacgccgt aggcgcctcc atcccatacc tgcacctgca ggctgtaact cgtgcgggtc
      181 tcgtagtcga gtgtctgttg aaccgtaatg acgccgttgg gagccatcga aaagacattc
      241 gtatctccgc cgatggctcg gtagtagaga ttacgataac cgggtgtcag cgtgtccggg
      301 tccgtggccg ggttgacgag attgatcgtg gtctgcgtcg ggttcgaaac gggatattcc
      361 tgcaccgata atgtctggtt ggaaagcgac ggcgcctcgt tcacatccgt aacgtttata
      421 ttgacggtcg ccaccgcgga attgccggaa ccgattgccc ccgcatccca cacccgcgcc
      481 tgtacgctgt agctctggcg agcctcgtag ttgaagctcg ccagcgtggt gatcgcaccc
      541 gtcgtctggt tgatcgcaaa ggcgccggtg tccccgccga tcagctcata acggaagtcg
      601 cgatacgccg ccgtcgcgtc gggatcgctc gccgtaagcg tcgttacgcc ggtaccggcc
      661 gcaagatttt ccgcaatcga gaacgtccca ccgctcagac tcggcgcttc gttcacgtcg
      721 atgatggaaa ccgtgaaagg gacatccttg gtcaaaccgc ttgtgtcggt aacccgcgcc
      781 gtaatggccg cgctgcttgc gctttcgtaa tcgagaagcg agccgttggc gaccgtgacc
      841 gcgccggtta cgctgttgat cgcaaaccgc ccgccggcag tgtcggtgag cgaataagtc
      901 agggtgtcgc catccggatc ctgaccggtc agcattccca ctactgttcc gttggcggcg
      961 ttttcgttga cgctaagaga acccgagata ccgcttggcc tggcgttgat ggttacgttg
     1021 atcgaatgat ccggtgtagt cgccgtcgaa ccgtttatcg cttcggtcga aatcgcccgc
     1081 acggtcaaag tgaagtctgt cgcccaattc gccggtccgg tgatggccaa accctgcaga
     1141 tcggctttgg tcaggctcca ggttccgccg ccgaggttgg ttcccttgtt gagcgaaagg
     1201 ccgcccggca gacccaggac ccagatctgc agggactccg aaccgtcggt atcgagcaga
     1261 tccgcgctga tcgagagcgg aagcgaaccg ccgtccagcg taccggaaag cgccgcaggt
     1321 gtcgtcaaac tcggcatgtc ggccacgggc gtgaccgtca gcgtgaacac cttctggtcc
     1381 atgcggccgg taaggtcgaa caccttcagc tgcgcataaa ccgtgccgcc gtaattcgcg
     1441 gacggcgcga tattcagtac ctggctcgag ccgttgagtg cgacattcca ggattgaacg
     1501 gccgtggccg tattctcctg ggtcagccct gcatcactgt aggtgcgcag ttcataatgc
     1561 agcagatcct tgctcgtgaa atcgtccgtc gcggtgatcg cgatcgcgag cgaattgtcc
     1621 tcgggcgtcg attgtgccga tatggcgccc aacacgggcg cctggttgat tccccagaag
     1681 gccgcccact ggcttgcata agtagaatcg gtcatcaggg caccgagggc cgccagatcg
     1741 gccggcttgg ccttcgtggc catcagcgcg gtcagaccat ccacatcgat gctgtaatgc
     1801 gtgctggtaa gcaccatttc gacgcggcgg ttggccggcg gcgggtcgtc ggcataccat
     1861 ccttcaactg tggcggtcga cgaactgccg acgatcgtga tcttgaggtc gttgccgata
     1921 cgctcgaacc acagccagga cgtgtccacg ccgttgtaat tgatctggtc gtgatcatcg
     1981 ccttccgcgt cgaaatcccg gatcgtgtca tgcccggagg ttgtatcgag tacgtaggag
     2041 tccgagcctt cgccgccttc caggatgtcg tctccggccc cgcccacgag ttcgtcaccc
     2101 gcgcccatgg cgcgcagaat gtcgttgccg gcgccgccat agaggtgatc gacgccctca
     2161 ccgccgatca agatgtcgtt gcccgcctcg ccgtagagcc atccaccatc gttgccgccg
     2221 gtaatggtat catcgccatc accgccatgg agttcgtccg caccgccgcc accgctgata
     2281 atgtcgtttc cagcaccacc gtcgatatat tcggccgcgg ccgtgcccgt gagcgtgtcg
     2341 gcgtacgatg agccgatcag ccgttcaata cccgagatcg tgtcgccttc cgcatctcca
     2401 cccgtaccga gccccgttgc caggttgacg ttcacacccg ccgtggagcc agcataagac
     2461 agcgtgtcgg tacctgttcc gccctggatg atgtctgccc ccggaccacc gtccaggaca
     2521 tcatcccccg cgccgccatc gagcatgtca ttgccggcgc cgccatagag catgtcatta
     2581 ccgtcgccgc ccaggagcgt gtcggcgcca tcgccgccga aaagctggtc gtttccatcg
     2641 tcagcctgga gataatcgtt ccccgcatcg ccgtagagcg tatcgtttcc ggaaccgccc
     2701 agaagatcgt cattgccgtc ggcgccttga agcgtatcgt cgccgcccat gccgcgcagg
     2761 atgtcgcggc cctgttggcc aacaagaacg tccgcaccgg gaccgccttc cagtacgtcc
     2821 gtctgatccg aaacggcaaa ggtgaaggtc cgcgtaaggg ccaacccctc cggtgcgccg
     2881 ttacggtctt ttaccgtcac atcgacgctc agggtcgtgc cggcttcgta atcgatcgaa
     2941 ctcatggcgc cggttttgag ccacaactcg ttcccattca ggatctcgaa gcgcggatcg
     3001 gaaacgctgt acgccagttg ggcgaagtcg ccgcttgtcg gcagatcggg atccgaggcc
     3061 gacagcacgc caagcttgat cgcaggcggc gtcggcccgt tgggatcagg ccggtcgcgt
     3121 tccgacacgt cggcgacaag cggcgtcaag ctcagcgagg tgggtgcttc gttgacatcc
     3181 gtgacgttca aggtgaagga cctagtcaac tcggggccgg tgccaagcgt cggatcatcc
     3241 acggttacgg caacggtgta gctttgttgt gcctcaaagt tgaggaccac gcccgccttg
     3301 agatagagcg cggaatcggc aatctcaaag gccgaggcat ccgggccggt taggccgtaa
     3361 acattggttc caagcgcatc gtcctcaacg ctgagatcgg cgactttggt gcgtgtcggg
     3421 gtcggtgtgt tttcggcaat ggatgttgtg gtattggaga agacaagatt cgtcggcgcc
     3481 tcgttgacgt ccgtgacgtc gatattgatc gtggcggcgc catacagccc cgcaccaaga
     3541 ctgccgccgt cccagacccg tgcctgaatg ctatagttcg aacgcgtttc ataatcgagg
     3601 ttggcgaggg tcgtgatttc gccggtcaac tggttgatcg tgaagctgtc cgtatcgccg
     3661 ccgatgagct cgtagcggaa gtcgcggaag t
//
LOCUS       DASWPU010000581         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_23899,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000581 DASWPU010000000
VERSION     DASWPU010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23899"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VGC27_12275"
     CDS             <1..218
                     /locus_tag="VGC27_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283386.1"
                     /translation="HRVLPRNTKEARLQRLAAVSPLPRRITFGCINVPASFFDGVVHP
                     AFTGTNGVVYILPEIKSIREVFFTSIS"
     gene            complement(471..1148)
                     /locus_tag="VGC27_12280"
     CDS             complement(471..1148)
                     /locus_tag="VGC27_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0283387.1"
                     /translation="MIDFYTAATPNGYKVSIALEELRLPYEVHALQFSKMEQKEDWFL
                     KINPNGRIPAIVDRDAGDFAVFESGAILLYLAEKTGKLIPPDAKGRSRVIQWLMFQMG
                     GIGPMMGQANVFYRYWPEKYQAAIDRYQNESRRLLEVLNGQLKDHEYLAGDYSIADIA
                     NWCWVRIHPWSGFSVDGLDHLQRWLKVVGDRPAVQRGVEIPKKTEEADAGDPAKRAAE
                     VQKILQR"
     gene            complement(1205..1663)
                     /locus_tag="VGC27_12285"
     CDS             complement(1205..1663)
                     /locus_tag="VGC27_12285"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY0283388.1"
                     /translation="MNDDEAIALALAEAQAAGLRGEVPVGAVLLSAEGEVLACDGNRI
                     LELRDPTAHAEMLVLRAAAQSLGNERLAGTALYVSLEPCAMCAGAVSMARIARLVFAA
                     EDPKGGAVLHGPRFFQQPTCHHRPEVTRAGDAEEAGRLLKSFFQARRTQT"
     gene            1677..2468
                     /locus_tag="VGC27_12290"
     CDS             1677..2468
                     /locus_tag="VGC27_12290"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY0283389.1"
                     /translation="MTDDSTEKGERIAQESPKGERIAKLIARAGICSRRDAEKLIADG
                     RVALDGEKVTTPATFVGPNQIVSVDGKPLGEPDPARLWRYHKPAGMVTTHRDPQGRPT
                     VFANLPKTLPRVVSIGRLDFNSEGLLLLTNDGEIARRLELPVGGWIRKYRARLFGKVS
                     QADLDKLAEGVTIGGVKYGPIVADLERSKGVHAWASVTLKEGKNREVKRVMESLGLRV
                     ARLIRVQFGPFHLGQLPEGAVEEIPAKLWREQLGIGRKKRAKESP"
     gene            2465..2764
                     /locus_tag="VGC27_12295"
     CDS             2465..2764
                     /locus_tag="VGC27_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283390.1"
                     /translation="MKPRALILICVLAAAGCGRWADLNSDAFAHGNATQERFTIDSRA
                     CDAKAENVRSYTITGIDADNVDKHAIFNRAYAACMKAKGYQEATSALNFWQAYNL"
     gene            2825..3094
                     /locus_tag="VGC27_12300"
     CDS             2825..3094
                     /locus_tag="VGC27_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283391.1"
                     /translation="MKKYVKPQAYAAETKDHRYAGTFEVLVPLPDRVKPHRVAQQFPS
                     LQAAEGWIHSEEGKDAIADVFEAHKPALAKAGPAKSAKTAKTAKR"
     gene            complement(3106..3582)
                     /locus_tag="VGC27_12305"
     CDS             complement(3106..3582)
                     /locus_tag="VGC27_12305"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0283392.1"
                     /translation="MPTYPLSGSCFCGAVPYRLLSAPMFVNCCHCRDCQRQSGGAFII
                     NAVIETDLIALLAGSPVPVSVLTESGQPHDIYRCPVCQTALWSDYGRRPAIRFVRVTT
                     LDDPSAFPPNAHTYTRSKLPWVVLPPGVPAFEEYYDMAKLYPPESLARRRAILGDE"
     gene            complement(3726..4196)
                     /locus_tag="VGC27_12310"
     CDS             complement(3726..4196)
                     /locus_tag="VGC27_12310"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0283393.1"
                     /translation="MPNYSGSCFCGAVKYYLESAPMFVNCCHCTECRRQTSSAFAINA
                     VIETARIALKPGSAEPVPVAVPTESGSPHDIYRCPVCQTALWSDYGRRPAIRFVRVAT
                     LDDPSAFPPNAHIFTRSKLAWVVLPPGVPAFEEYYDMAKLYPPDSLARRRAIDG"
BASE COUNT          887 a         1427 c         1398 g          776 t
ORIGIN      
        1 tgcatcgtgt gttgccaaga aataccaagg aggcgcgcct gcaacgactc gcggcggtat
       61 cgccccttcc ccgccgaatc acttttggct gcatcaatgt tccggcaagt ttcttcgacg
      121 gcgttgtgca ccccgcgttt acggggacga atggcgtcgt ctatattctg ccggaaatca
      181 aatcgatccg cgaagttttc ttcacctcta tctcataggg gccggcgcgt tcgcgcccac
      241 gccttcccgt acaggctcgc ctgcctcgtg aatggcattc gaatcacccc aggcagtaca
      301 acctgtacct agtgtcgtgg tttcgaagtt cgcagcatgc gccatatcgg gctgcaagcg
      361 aacttcgaaa ccaaaacgac actaggattc aataggttgc tagtgtcctt tcgattccga
      421 aattcgcccc atctctatat cggctgcggg cgaatttcgg aatcgggaca ctagcgctgc
      481 aatatctttt ggacttcggc ggcgcgtttg gcgggatcgc cggcatcggc ctcctcggtc
      541 tttttgggaa tctcgacgcc gcgctgcacg gcaggccggt cgccgacgac cttcagccag
      601 cgttgcagat gatcgaggcc atccaccgaa aagccggacc agggatggat gcgcacccag
      661 caccagttgg cgatatcggc gatggagtag tcgccggcca gatattcgtg gtccttgagc
      721 tgcccgttca acacttctaa cagccggcgc gattcgttct gatagcggtc tatggccgcc
      781 tgatatttct ccggccaata gcggtagaac acgttcgcct ggcccatcat cgggccgatg
      841 ccgcccatct ggaacatcag ccattggatg acgcgcgagc ggcccttggc gtccggcgga
      901 atgagcttgc cggtcttctc ggccagatag agcaggatcg cgccggattc gaagacggcg
      961 aaatcgcccg cgtcgcgatc gacgatggcc ggaatgcgcc cgttgggatt gatcttcagg
     1021 aaccagtctt ccttttgctc cattttgctg aattgcagcg catgaacttc gtagggcaat
     1081 ctcagctctt cgagcgcaat ggaaacttta tagccgttcg gcgtggcggc cgtgtagaag
     1141 tcgatcatgg catcctttcc gttcaaaccg cgctacctgc aatgtagggt gcggcccgcc
     1201 aggatcaagt ttgagtccgt cgcgcctgga agaaggactt gagcaaccgc ccggcttcct
     1261 ctgcgtcacc ggcccgcgtg acttccgggc gatgatggca agtgggctgc tggaagaagc
     1321 gcgggccgtg caagaccgcg cctcccttag ggtcttcggc cgcgaacacc agccgggcga
     1381 tgcgcgccat ggatacggcg cccgcgcaca tggcacaggg ttccagcgag acgtaaagcg
     1441 cggtcccggc cagccgctcg tttcccaggc tttgggcggc ggcgcgcagg accagcatct
     1501 cggcatgggc ggtcggatcg cgcagttcca ggatacggtt accgtcgcag gccagcactt
     1561 cgccctcggc ggacagcagc accgccccga ccggcacttc accccgcaga ccggccgctt
     1621 gggcttcggc cagcgccagt gctatggctt cgtcgtcatt catgcggatt ccttctttga
     1681 ccgacgatag cacggagaaa ggcgagcgca tcgcacaaga gagcccgaag ggcgaacgca
     1741 tagcaaagct gatcgcgcgg gcgggcattt gttcgcgccg cgacgccgag aagctgatcg
     1801 ccgacggtcg cgtggcgctc gacggcgaaa aggtgacgac acccgccacc ttcgtcggcc
     1861 cgaaccagat cgtctcggtg gacggcaagc ctctgggcga acccgatccg gcgcggctgt
     1921 ggcgctatca caagccggcc ggcatggtga cgacccaccg cgatccgcag ggccgcccga
     1981 ccgtcttcgc caacctgcct aagaccctgc cgcgcgtggt ttccatcggc cggctggatt
     2041 tcaattccga agggctgctg ctgcttacca acgacggcga gatcgcgcgg cgcctggagc
     2101 tgcccgtggg cggctggatc cgcaaatacc gcgcccgcct gttcggcaag gtcagccaag
     2161 ccgatctgga caaactcgcc gaaggcgtca ccataggcgg agtcaaatac ggccctatcg
     2221 tcgccgacct ggagcgctcc aaaggagtcc atgcctgggc cagcgtcacc ctgaaggaag
     2281 gcaaaaaccg cgaggtcaag cgcgtgatgg aaagcctggg cctgcgcgtg gcgcggctga
     2341 tccgcgtgca gttcggaccg ttccatctcg gccagttgcc cgaaggcgcg gtagaggaaa
     2401 tccccgccaa gctgtggcgc gaacagctcg gcatcggacg gaagaagcgt gctaaggaga
     2461 gtccatgaaa ccgcgtgctt tgatcctgat ctgcgtcctg gccgcggcag gctgcggccg
     2521 ctgggccgac ctcaactccg acgccttcgc ccacggcaac gccacgcagg aacgattcac
     2581 catcgacagc agagcctgcg acgccaaggc cgagaacgtg cgttcctata ccatcaccgg
     2641 catcgatgcc gataacgtgg acaaacacgc gattttcaat cgcgcttatg ccgcctgcat
     2701 gaaagccaag ggctaccagg aggcaacctc cgcgctcaat ttctggcaag cttacaatct
     2761 ttaaggttac acgcccggcc cagggtgata tgcacgccgg cttgccgcgc ggaaggaccg
     2821 gaccatgaaa aaatacgtca agcctcaggc ctacgccgcc gaaactaagg atcaccgcta
     2881 cgccggcact ttcgaggttc ttgtacccct gcccgaccgc gtcaagccgc atcgcgtcgc
     2941 ccagcaattc cccagcctgc aagccgccga aggctggata catagcgagg aaggcaaaga
     3001 cgccatcgcc gacgtcttcg aagcccacaa gccggcgctg gccaaagccg ggcctgccaa
     3061 atcagcgaag acggccaaga ccgccaagcg ctagggcgaa aggggctact catcccccag
     3121 gattgcccgg cggcgggcca ggctttcggg gggatagagt tttgccatgt cgtaatattc
     3181 ctcgaatgcg ggcacgccgg gcggcagcac cacccagggc agcttcgagc gcgtgtaggt
     3241 gtgcgcgttg ggcggaaacg cggacggatc gtccagcgtc gtcacgcgca cgaaacggat
     3301 cgcgggacgg cggccgtaat cgctccatag cgcggtctgg cagacggggc agcgatagat
     3361 gtcgtgcggc tggccgcttt cggtgagcac ggacacggga acgggcgaac ccgccagcag
     3421 cgcgataagg tcggtctcga tcaccgcgtt gatgatgaag gcgccgccgg actggcgctg
     3481 gcaatcgcgg caatggcagc agttcacgaa catcggcgcg gacaggaggc gataaggcac
     3541 cgcgccgcaa aaacaggaac cggaaagggg gtaagtgggc ataagggcct cccgccgggc
     3601 tgcatacccg acacttcatc cgtagacccc aacgattcgc gacactatag taattatgag
     3661 gcgcatgcgc cccttatgta gccgacggta gacgataccc gcagcgcgca ttcgggcggc
     3721 gggggctatc catcaatcgc ccggcggcgg gccaagctat cgggcgggta aagcttcgcc
     3781 atgtcgtaat attcctcgaa ggcgggcacg ccgggcggca acacgaccca agccagtttc
     3841 gagcgcgtga agatgtgcgc gttgggcggg aacgcggagg gatcgtccag cgtcgccacg
     3901 cgcacgaagc ggatcgcggg gcggcggccg taatcgctcc acagcgcggt ctgacagacg
     3961 gggcagcgat aaatatcgtg cgggctgccg ctttcagtgg gtacagccac gggaacgggt
     4021 tcggccgatc cgggcttgag cgcgatgcgg gcggtctcga tcaccgcatt gatggcgaag
     4081 gcgctgctcg tctgccgccg gcactcggtg cagtggcagc agttcacgaa catcggcgcg
     4141 gattcgagat agtacttcac cgcgccgcag aaacaggaac cggaatagtt gggcattagg
     4201 acctccccca ttggcgcgag gataatccaa caggctattc tgccaagggc acgtaggggc
     4261 ataaccccca ctgaaaattc gcaacgcgaa tttttcgccc ccctctcaag ggggaggcaa
     4321 ttgccccctt cggcgcgcgc acgctgtcgc tatgcgccgc accacttccc ccgcaacccg
     4381 tcttcgctac agcttcggtg ggcatagcgg aggtgaagaa agagcggcac accccctccg
     4441 cttcgctcgc gagtgctcgc tcggcacctc ccccgcttcg cgggggag
//
LOCUS       DASWPU010000582         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_109521,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000582 DASWPU010000000
VERSION     DASWPU010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109521"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2696)
                     /locus_tag="VGC27_12315"
     CDS             complement(<1..2696)
                     /locus_tag="VGC27_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0283394.1"
                     /translation="MKLRTRSLLMGTTLLAGIVAFAAPTAALAQDSDQAMETVVVTGY
                     RASLESSTNAKRDSVNFSDTVFAEDIGKFPDTNIAEAFNRIPGVTITRENDGSGMRVA
                     IRGLDTNHVKITLNGAVVSTASTGNTDASGANREVDLNIFPIELFSQLTVSKTSTADQ
                     LEGGASGVISMRSLRPFDNPGTHLSYNVQLSDYENNGNPGYRGTLIGSWTDGPFGVLV
                     GLSGQVNRVLVTGYEGAFNSLTTPNLTNVMYYGNSTTPCAATTLTSPGSSSTAGANTC
                     NSIGGNADWNVPYDTSHDTVSNNVATGVPFNTAALAAMSAAAGYPITTGMTIDRQMLL
                     DLNPGLSIDQVSNAIVPRTGRPMFERGLRNRYNGILSLEYRPSDDLHFYFDSILGVIE
                     NSLDREDLFWIARNGFAVPMNMQVDEHNVLLKGDMANAAVSLEARPYKERSDYFSLNP
                     GMDWQVTDLLHVDAQVNYSRAHFFRDSPTFLFSTPLGILHYDNTGSVPVFTMDGLPGA
                     NGLQDPANYGWYGGSDLRLQQERRYQYTKGAHANAKYGGDEFAVQVGAAWDQAYRLIR
                     GYDNGRAFANANCGNAMSVVLPGPNTTVGCGVTPGAVPAYPGWGTGFSENLGAFPTAG
                     TPALPHADVPNYLLPGPNGFARADYAKLKAVTNYEYFAHNPSLGAAANLQDPGRTNFS
                     RGTNTNISSGIIDEKTTGFYGEIIGTLHRGDQKLRYNIGARWIRTQQTLTGYTLLDDP
                     RNANGTADPADDQQNGGQYPQLVLPGTLSSSYSAFLPSVNLVWEIYDDFQIRGAVSRT
                     MTRANPASMLPQLSGGGSDAKNWNLGNPDLRPFYSTNIDLGAELYTGGEGYIGVGVFK
                     KMISGFPNNFTSQQPFSYLAPLGVTYDVLSPAQQDSIAASGGP"
BASE COUNT          482 a          787 c          908 g          550 t
ORIGIN      
        1 ggtccgccgg acgcagcgat ggaatcctgc tgcgccgggc tcagaacatc gtaggtgacg
       61 cccagcggcg ccagatagct gaacggctgt tgtgaggtga agttgttcgg gaatccggag
      121 atcattttct tgaagacgcc aacgccgata tagccttcgc cgccggtata gagttcggcg
      181 ccgagatcga tgttggtcga gtagaacggc ctcaagtccg ggttgccaag gttccagttc
      241 ttagcgtcag agccgccgcc ggacagttgc ggaagcatgc tggccggatt tgccctggtc
      301 atggtgcggg acaccgcgcc cctgatctgg aagtcgtcgt aaatctccca caccaggttc
      361 acggagggca ggaacgccga gtaggaactg ctcagtgtgc ccggcaggac caactgcgga
      421 tactgcccgc cgttttgctg gtcgtcggcc gggtccgccg tgccgttcgc gttcctcggg
      481 tcatccagca aggtgtagcc ggtcagcgtc tgctgcgtac ggatccagcg ggcgccgata
      541 ttgtaccgga gcttctggtc gccgcgatgc agcgtaccga tgatctcgcc gtagaagcca
      601 gtcgtcttct cgtcgatgat gcccgagctg atgttggtgt tggttccgcg gctgaagttc
      661 gtgcggccgg gatcttgcag gtttgcggcc gcgccgagcg acggattgtg cgcgaagtat
      721 tcgtagttcg tcaccgcctt cagcttagcg tagtccgccc ttgcgaagcc gttggggcca
      781 ggcagcagat aatttgggac gtcggcgtgc gggagtgcgg gagtgcccgc cgtcgggaac
      841 gcaccaaggt tctcggagaa gccggtaccc caaccgggat aggcgggaac tgcgccgggc
      901 gtgaccccgc agcccaccgt cgtgttcggg ccggggagta cgaccgacat ggcgttgccg
      961 cagttggcat tggcgaacgc cctgccgttg tcatagccgc ggatcagacg ataggcttgg
     1021 tcccaggccg caccgacctg gacggcgaat tcgtcgccgc cgtacttcgc gttggcatgg
     1081 gcgcccttgg tgtactgata acggcgctcc tgctgcagac gaaggtcgga gccgccgtac
     1141 cagccgtagt tcgccggatc ctgcaggccg ttggcgcccg gcagcccgtc catcgtgaag
     1201 accggaacgg agcccgtgtt gtcgtagtgc aggatgccaa gcggcgtcga gaacaggaag
     1261 gtcggcgaat cgcggaagaa gtgcgcacgg ctgtagttca cctgcgcatc gacatgcagc
     1321 agatcggtga cctgccagtc catgcccgga ttgaggctga agtagtccga tctttccttg
     1381 tatggacggg cttcgagcga aacagccgca ttcgccatgt cgcctttgag gagcacgttg
     1441 tgctcatcga cttgcatgtt catcggaacg gcgaagccgt tgcgggcgat ccagaacagg
     1501 tcttcgcggt cgaggctgtt ctcgattacg cccaggatgc tgtcgaagta gaagtgcaga
     1561 tcgtcgctcg gacgatattc cagcgaaagg atgccattgt agcgattgcg gaggccgcgc
     1621 tcgaacatcg ggcgtccggt acgcggaacg atggcgttac tgacctgatc gatgctcagg
     1681 cccgggttca aatccagcag catctgacgg tcgatcgtca tgcccgtggt gatcggatag
     1741 ccggctgcgg cactcatagc ggcaagagcc gccgtattga acggcacacc ggtggccacg
     1801 ttgttgctca cggtatcgtg gctcgtgtcg taagggacgt tccagtcggc gttgccgccg
     1861 atgctgttgc aggtattcgc accggccgtg ctgctcgagc cgggagacgt gagcgtcgtc
     1921 gcggcgcacg gcgtcgtcga gttgccgtag tacatcacgt tggtcaggtt cggggtcgtg
     1981 aggctgttga aggcgccttc atagcccgtg accaaaacgc ggttcacctg gccggacagg
     2041 ccgaccagca cgccgaacgg accatcggtc cagctgccga tcaacgtgcc gcgatagccc
     2101 ggattgccgt tattctcgta atccgacagc tgcacgttgt acgagaggtg cgtacccgga
     2161 ttgtcgaacg gacgcaggct gcgcatgctt atcacgccgg aggcgccgcc ttcgagttgg
     2221 tcggcggtgg acgtcttgct caccgtcaac tgcgagaaca gttcgatcgg gaagatgttg
     2281 aggtcgactt cgcggttggc gccgctggcg tcggtattgc cggtagaggc cgtcgaaacg
     2341 accgcgccgt tcagcgtgat cttcacatgg ttggtgtcga ggccgcgaat ggcgacgcgc
     2401 atgcccgaac cgtcgttttc acgggtgatc gtgacgcccg gaatgcggtt gaaagcttcg
     2461 gcgatgttgg tgtccgggaa cttgccgatg tcttcagcga agactgtatc ggagaagttc
     2521 accgaatcgc gcttggcatt ggtcgaagac tccaagctgg cgcgatagcc cgtcaccacg
     2581 acggtttcca tcgcttggtc ggagtcctgg gccagagccg ccgtgggcgc ggcaaacgcc
     2641 acgatccctg ccagcagcgt cgtgcccatg agcaacgagc gcgtgcgtaa tttcacacta
     2701 tccatattcg gatgcatgct ttaatcc
//
LOCUS       DASWPU010000583         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_36244,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000583 DASWPU010000000
VERSION     DASWPU010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36244"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGC27_12320"
     CDS             <1..312
                     /locus_tag="VGC27_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283395.1"
                     /translation="VGRGLDRETVRRIESVAIGDFKPELTFVLDLPAEEGLRRAGARA
                     SAESRFEHFDIGFHERLRQAFLDIAKRAPDRCRVIDASEDEDTVAQAIWQAVAARFAL
                     S"
     gene            317..1369
                     /locus_tag="VGC27_12325"
     CDS             317..1369
                     /locus_tag="VGC27_12325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY0283396.1"
                     /translation="MAKRSRPPVETDRIEGTDHPRRTARLIGQDFALAIVSRALRSGR
                     PPQAWLICGPPGVGKATLAYRIARYLLAYGASGRGPADLSVPTNDPAFVQVAAGSHPG
                     LLVLKRSTDPDTGKTMNVLSVGEVRRLSGFFGLTSGAGGWRVALVDTADDMNDAAANA
                     LLKLLEEPPSRAMLLVLSNTPGRLLPTIRSRCQRLNLRPLGEPDMEAELGRLLPEQTA
                     EERASLARLAHGSLGAALTLAGGEGLVLAKEADRLIDWAAQPDIPALFALAEKLSRIT
                     DGLDMLGAFLTQALAERIRARALGNQPHLTRWVGALEKLSNNFARTGALHLDPRQTLL
                     AAAREMNAAVRRAGVV"
     gene            1613..2425
                     /locus_tag="VGC27_12330"
     CDS             1613..2425
                     /locus_tag="VGC27_12330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY0283397.1"
                     /translation="MLVDSHCHLEYPALASEIDAVMARAHAAGVGACVSISTKLETFE
                     TVRAMAALFDNVWCSVGVHPHEAAAEPLTGAAPLLERADDPKVIGIGETGLDYFYDHS
                     PRAVQRTNFRAHIEASRLTKLPLIVHTRDAEDDTVAILREETAKSAFPGVLHCFTGTA
                     TLAQAALDLGFTISASGIVTFKKSEALRAVLKEVPLDRLLVETDAPYLAPVPHRGKSN
                     EPSFVAHTAAALAELKGVTAETLARATTDNFFRLFTKASRPQSPFAKAAGDT"
     gene            2422..2559
                     /locus_tag="VGC27_12335"
     CDS             2422..2559
                     /locus_tag="VGC27_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283398.1"
                     /translation="MTGKLEITILGCGSSGGVPRLGEFSASDSMVLFIRASRLNSLIS
                     A"
BASE COUNT          429 a          874 c          864 g          451 t
ORIGIN      
        1 gtggggcggg gactcgatcg cgagacggtg cgccggatcg aatccgtggc catcggcgac
       61 ttcaagccgg agctgacgtt tgttctcgat ctgccggcgg aggagggcct gaggcgcgcc
      121 ggcgcccgcg cctcggctga atcgaggttc gagcatttcg acatcggctt ccacgaacgc
      181 ctgcggcaag cgtttctcga tatcgccaag cgggcgccgg accgctgccg ggtgatcgac
      241 gcgagcgagg acgaagacac ggtggcgcag gcaatctggc aagccgtggc cgcgcgattt
      301 gcgctaagct aggcgcatgg ccaaacgctc ccgcccgccc gtcgagaccg accgcatcga
      361 aggcacggat catccgcgcc ggacggcgcg cctgatcggg caggacttcg cgttggcgat
      421 cgtttcgcgc gcgctgcgca gcgggcggcc gccgcaagcc tggctgatct gcggaccgcc
      481 cggcgtgggc aaggcgacgc tcgcctatcg catcgcgcgc tatctgctgg cctacggcgc
      541 cagcggtcgc ggcccggccg atttgagcgt gcccacgaac gatccggctt tcgttcaagt
      601 cgcggccggt tcgcatcccg gcttgctagt gctcaagcgc agcaccgatc ccgatacggg
      661 caagacgatg aacgtattgt cggtcggcga agtgcggcgg ctgtcgggct ttttcggtct
      721 gacctcgggc gcgggcggct ggcgcgtcgc cctggtcgat accgccgacg acatgaacga
      781 tgccgccgcg aacgcgctgc tgaagctgct cgaagagccg ccgtcgcgcg ccatgctgct
      841 ggtgctgtcg aatacgccgg gacggcttct gcctacgatc cgttcgcgct gccagaggct
      901 gaacctgcgg ccgctcggcg aacccgatat ggaggcggag cttgggcgcc tgctgcccga
      961 acagacggcg gaagagcgcg cctcgctggc gcggctggcg cacggctcgc tgggcgcggc
     1021 gctgacgctg gcgggcggcg agggactcgt gctcgccaaa gaagccgacc ggctgatcga
     1081 ctgggcggcg cagccggata ttccggcgct gttcgccttg gccgaaaagc tttcacgcat
     1141 caccgacggc ctggacatgc tgggcgcgtt cctcacccag gcgctggccg agcgcatccg
     1201 cgcgcgcgcg cttggcaacc agccccatct aaccagatgg gtcggcgcgc tggagaagct
     1261 gagcaacaac ttcgcgcgca ccggcgcatt gcatctcgac ccgcgccaga cgctattggc
     1321 ggcggcgcgc gaaatgaacg cggcggtaag gcgggccggc gtggtgtagg agaaggcttt
     1381 ggaacaagat tatgaaccat cctattcgtc atggccgccc ttaccgtctt cgctaaagct
     1441 atggcggccg agtgagcctg ctctcgcccg ccgaagcctt ggcgtaggcg ggaggcgtcc
     1501 atccaggcat agaggtgacc tgccgtgaaa ttgactgctg gatggccggg tcaagcccgg
     1561 ccatgacgaa tttggagatt caatcagctt tgataccgct ctaggattcc cgatgctcgt
     1621 cgatagccat tgccatctgg aatatcccgc gctcgcttcc gagatcgacg cggtgatggc
     1681 gcgtgcgcac gccgccggcg tgggcgcgtg cgtcagcatt tccaccaagc tggagacctt
     1741 cgagaccgtg cgcgccatgg cggcgctctt cgacaatgtg tggtgctcgg tcggcgttca
     1801 tccgcacgag gccgccgccg aaccgctgac cggcgccgcg cctctgctcg aacgggcaga
     1861 cgatcccaag gtgatcggca tcggcgagac cgggctggat tatttctacg atcattcgcc
     1921 gcgcgccgtg cagaggacga acttccgcgc ccacatcgaa gcctcgcgcc tgaccaagct
     1981 gccgctcatc gtccataccc gcgacgcgga agacgacacg gtggcgatcc tgcgcgagga
     2041 aaccgcgaaa agcgcctttc ccggcgtatt gcattgcttc accggaacgg cgacgctggc
     2101 gcaggcggcg ctggatctgg gcttcaccat ctcggcctcc ggcatcgtga cgttcaagaa
     2161 atccgaagcg ctgcgcgcag tcctcaagga ggtaccgctg gaccggctgc tggtggagac
     2221 cgacgcgccc tacctggcgc ccgtgcctca tcgcggcaaa tccaacgaac cgtccttcgt
     2281 ggcgcacacc gccgcggcgc tggcggagct gaagggcgtc acggccgaaa cgctggcgcg
     2341 cgccaccacc gataatttct tccgtctctt cacaaaagca tcacgcccgc aatctccctt
     2401 cgctaaagct gcgggggaca cgtgaccggc aaactcgaaa tcaccatcct cggctgcggc
     2461 tcgtccggcg gcgtgccgcg cctgggcgag ttctccgcaa gcgattcgat ggtgctcttt
     2521 atcagagctt cgcgcctgaa ctcgttgatc tccgcgtgag tcctcggtcg ttccttcgcc
     2581 ttacgcttcc tgcccagcat gcttagacct ctcgtctt
//
LOCUS       DASWPU010000584         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_66957,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000584 DASWPU010000000
VERSION     DASWPU010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66957"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VGC27_12340"
     CDS             <1..933
                     /locus_tag="VGC27_12340"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY0283399.1"
                     /translation="RVAESMYWMSRYLERAEHTSRLIAVKLESMVEQSHEDADSSWGR
                     VVAALSAEEHVPAQPDAFAITQGLAFDRKNPSSLLSSLHNARDNARQVREQLSMEVWE
                     NLNRLYLRLQPMTMDGVWAHHPARLFREVLEDLHTLGGVTYSTLSHGEGWHFLELGRY
                     IERAQLVCRLLDLHFGAAAIGEAPKYFDWLVLLKFCSAFEPYCKAHTAAIRPEKIAEF
                     LLCDGEFPHSVRFSVDRVCEALLHVAPGAPPLRRAGVERLAGRLKASVDFTQIEELMS
                     GSIAPFLEDISRQCEQIHEAVYASYIAYGAETVL"
     gene            1086..2054
                     /locus_tag="VGC27_12345"
     CDS             1086..2054
                     /locus_tag="VGC27_12345"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0283400.1"
                     /translation="MFYSIRHVTRFRYSAPVRESVMELRMQPRSEGPQALRSFQLNTN
                     PRAQLYAYTDHFGNAVYHFNVLRAHEELRIEAQSVIELSRIPALPEAADALEWERFNS
                     YNLTDDHFDLLEPSRFAFSSPALVRFMAEQGLENPRGDPLTALRFLNRAIHNAFAYEA
                     GITQVHSPIDHALEERRGVCQDFAHVMIAIARRWGIPARYVSGYLYHRPQARDRSGAD
                     ATHAWAEAFLPSLGWIGFDPTNDILTGERHIRAAVGRDYADVPPTRGTFKGVAESELA
                     IAVSVEPTQAPVRHEDFLRVARPMSSPPPTPSMPERLYHQQQQQQQ"
     gene            complement(2056..>2641)
                     /locus_tag="VGC27_12350"
     CDS             complement(2056..>2641)
                     /locus_tag="VGC27_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar protein FlbA"
                     /protein_id="HEY0283401.1"
                     /translation="MGTTLQLLRSRLEDFPHRAFLRPDPKRAAEFREKLRAGGPGPTV
                     GICWRSMMLDAKRAKYYSALDMWGPILKTPGVRFVNIQYGECEAELARARELHGVEIQ
                     RFDGLDLTNDIDGAAALSAALDLAISAPTAAAACAAGVGTKVWFLTAGRTWPQLGTDE
                     YPWYRKSHVFSPEKFGDWNELMPRIGERLAAFAG"
BASE COUNT          438 a          907 c          799 g          497 t
ORIGIN      
        1 cgcgtcgccg aaagcatgta ctggatgagc cgctatctgg agcgggccga acacacctcc
       61 cgtctgatcg cggtcaaact cgaatccatg gtcgaacaat cgcacgagga cgccgattcc
      121 tcctggggcc gcgtcgtcgc ggcgctgtcg gccgaggaac acgtccccgc gcagcccgat
      181 gccttcgcca tcacccaggg cctggccttc gaccgcaaga atccgtcctc gctgctgtcg
      241 tccctgcaca acgcgcgcga caacgcccgc caagtgcgcg aacaattgtc gatggaagtg
      301 tgggagaatc tgaaccggct ctatctgcgg ctgcaaccga tgacgatgga tggcgtttgg
      361 gcgcatcacc cggcgcgcct gtttcgcgag gtcttggaag accttcacac gctcggcggc
      421 gtgacctatt cgacgctcag ccacggcgaa ggctggcact tcctcgaact cggccgctat
      481 atcgagcgcg cccagctcgt ctgccgcctc ctcgacctgc atttcggcgc ggccgccatc
      541 ggcgaagcgc cgaaatattt cgactggctc gtgctgctga aattctgctc ggcgttcgaa
      601 ccctactgca aggcccacac ggccgcgatc aggccggaga agatcgcgga atttcttctg
      661 tgcgacggcg aattcccgca ttccgtccgc ttttccgtcg atcgcgtctg cgaggcgcta
      721 ctgcatgtgg cgcccggggc gccgccgctg cgccgcgccg gcgtcgagcg cctggccggc
      781 cgcctgaaag cctcggtcga tttcacccag atcgaggaat tgatgagcgg ctccatcgca
      841 cccttcctcg aagacatcag ccggcaatgc gagcagattc acgaggccgt ctacgcctcc
      901 tacatcgctt acggcgcgga gacggtgctg tgacgcaaat agcgaatggc gaatggcgag
      961 tagggaattg gcgccccctt cgcctcgctg cactcggcac ttcccccgct tcgcgggggc
     1021 agaaacccct attcgctact ttctattcgc tattcgcaaa tccacgatag gaattgcagg
     1081 ttcaaatgtt ctactccatc cgccatgtca cgcgttttcg ctattcggcg ccggtgcgcg
     1141 aaagcgtcat ggaactgcgc atgcagccgc gttcggaagg gcctcaggcg ctgcgctcgt
     1201 tccagctcaa caccaatccg cgggcgcagc tttacgccta caccgatcac ttcggaaacg
     1261 cggtctacca tttcaacgtc ctgcgcgcgc acgaggaact gcgcatcgaa gcccagtcgg
     1321 tcatcgagct ttcccgcatc cccgcgctgc cggaggccgc cgatgcgctc gaatgggaac
     1381 gcttcaattc ctacaatctg accgacgatc atttcgatct cttggagcct tcgcgcttcg
     1441 ccttttcctc accggcgctg gtccgcttca tggccgagca gggcctcgaa aacccgcgcg
     1501 gcgatcctct gaccgccttg cggtttctca accgggccat ccataacgcc ttcgcttacg
     1561 aggccggcat cacccaggtt cattcgccga tcgatcatgc gctcgaggag cggcgcggcg
     1621 tctgccagga tttcgcgcat gtcatgatcg ccatcgcgcg gcgctggggc atcccggcgc
     1681 gctacgtctc cggttacctc tatcaccgtc cgcaggcgcg tgaccgttcg ggagcggatg
     1741 cgacccatgc ctgggcggag gcctttctgc cttccctggg ttggatcggt ttcgacccca
     1801 ccaacgacat tctcaccggc gagcgccaca tccgtgcggc ggtcggccgg gactatgccg
     1861 acgtgccgcc gacgcgcggc accttcaaag gtgttgcgga aagcgagctt gccatcgcag
     1921 tttcggtcga gccgactcag gcgccggtcc gccatgagga cttcttgcgc gtcgcccgcc
     1981 cgatgtcctc gccgccgccc acgccgtcga tgcccgagcg gctctatcat cagcagcagc
     2041 aacagcagca gtgatctagc ccgcgaaggc ggcgagacgc tcgccgatcc gcggcatcag
     2101 ctcgttccaa tcgccgaatt tttccgggct gaagacgtgg ctcttgcggt accaggggta
     2161 ttcgtcggtg ccgagctgcg gccaggtgcg cccggcggtc aggaaccaca ctttggtccc
     2221 gacgccggcg gcgcacgccg ccgctgcggt gggcgcggag atcgccagat cgagcgcggc
     2281 cgatagggcc gctgcgccgt cgatgtcgtt ggtgaggtcg aggccgtcga agcgctgaat
     2341 ctcgacgccg tgcagttcgc gcgcgcgcgc cagctccgct tcgcactcgc cgtattggat
     2401 attgacgaaa cggacgcccg gcgtcttgag gatcggtccc cacatgtcca gcgcgctgta
     2461 gtatttcgcg cgtttggcgt cgagcatcat cgagcgccag caaatgccga cagtgggacc
     2521 ggggcctccc gctcgcagct tttcgcggaa ctccgccgcg cgcttgggat cgggcctgag
     2581 gaatgcccga tggggaaaat cctccaggcg gctgcgcagc agctgcaagg tcgtgcccat
     2641 c
//
LOCUS       DASWPU010000585         3038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_102653,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000585 DASWPU010000000
VERSION     DASWPU010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102653"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGC27_12355"
     CDS             <1..234
                     /locus_tag="VGC27_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283402.1"
                     /translation="VPGLKANIAGVNFVAPKPSLDPFDASHMLKSWRSRIATPIGGLF
                     LCGKAAEPVPALSCRAARIAAAMALTQLEGERR"
     gene            231..1328
                     /locus_tag="VGC27_12360"
     CDS             231..1328
                     /locus_tag="VGC27_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283403.1"
                     /translation="MTALRATPFHVRAAETNRLNAWENRNGFTLASGDPGEALAARFG
                     AIMADISWQARAAITGARAGEFVSRLLTRDASRLQPGEALKALWLNDAGGVRGLGTIV
                     RLGAESFVLVSAVEDFAWLTEAAALYGVAVRDVTAEEGVLALIGPAAGKVLAAAGLET
                     DPGPLALRKFFWRGLDVTISRLNDGYELWCAPDDALIVWDRIAKAGSVFALCPAGQRA
                     LDIIDLESGIVRPGRDFAPARDGFSPEPSPQSLGLSGLVDRDCIFNGRAGYLAGGGGK
                     ALAGILFDSETPMPQTVLMRGNLAVGSTLDSLVSPALRRAAALAILETDAAAPGTALT
                     AGGAACRTCALPFLPTPAPLAPATENPPPGV"
     gene            1358..1903
                     /locus_tag="VGC27_12365"
     CDS             1358..1903
                     /locus_tag="VGC27_12365"
                     /inference="COORDINATES: protein motif:HMM:NF004113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0283404.1"
                     /translation="MKIDPDAELVARAGKGDRAAAQALMVRHLPKMLTLARRMLRDRT
                     EAEDAVQEAFLKLWTHAARWRPGQAKFETWLYRVTLNQCYDRLRRKPTLELEAAAKVP
                     DPAPGPETELENAGLAAEVEAALAELPERQRAAILLCHHEERGNMEAAEILGISVEAI
                     ESLLARGRRTLRMKLAHLKDG"
     gene            1913..2305
                     /locus_tag="VGC27_12370"
     CDS             1913..2305
                     /locus_tag="VGC27_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283405.1"
                     /translation="MAEKDPIDRASATAEAQSDAAFAARALTELPSVPVPGTLEARIL
                     ADFDAVAAKRKASLWARVLRPARGLRDAIWPDAPAWKPASVFALSLLIGLAAGTLVPS
                     SALSGAGSVQDETAVLDTVPNLDIFGDF"
     gene            2307..2831
                     /locus_tag="VGC27_12375"
     CDS             2307..2831
                     /locus_tag="VGC27_12375"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEY0283406.1"
                     /translation="MSNGSPQGKSNVALIISLCINLVLAGVIAMGGYRAFHHGPMFGN
                     TPGFEKGYGGGHGQRPMGSRALMHLLPAETGKVKAVADAHRDRVFQLRSEARQARRDV
                     LRTFASPDFSQQAFDRALERVRNADAALEAEELKVVSESVAALTPDERRAAVERAREH
                     RGFGMHRGGRSRGF"
BASE COUNT          479 a          970 c         1057 g          532 t
ORIGIN      
        1 gtgccgggct tgaaggccaa tatcgccgga gtgaatttcg tcgcgccgaa gccgtctctc
       61 gatccattcg acgcctccca tatgttgaaa agttggcgaa gccgcatcgc cacgccgatc
      121 ggcggattgt tcctgtgcgg caaagccgcc gagccggtgc ccgctctgtc gtgccgcgcg
      181 gcgcgcatcg ccgccgccat ggcgcttacg cagttggagg gagagagaag atgaccgctc
      241 tccgcgctac gccgtttcat gtgcgcgccg ccgaaaccaa caggctcaac gcctgggaaa
      301 accggaacgg tttcacgctg gcttcgggcg atccgggcga ggcgctggcg gcgcggttcg
      361 gcgcgatcat ggcggatatc tcgtggcagg cgcgcgcggc gatcaccggc gcgcgcgcgg
      421 gcgaattcgt gtcgcgcctg ttgactcgcg atgcctcccg gcttcagccc ggcgaagcgt
      481 tgaaagcctt gtggctgaac gatgcgggcg gcgtgcgcgg cctcggcacg attgtgcgcc
      541 tgggcgcgga atcgttcgtg ctggtttcgg cggtggagga tttcgcctgg ctcacggagg
      601 cggcggcgct ttacggcgtg gcggtgcgcg acgtcaccgc ggaggaaggc gtgcttgcgc
      661 tcatcggacc cgccgccggc aaagtgctcg cggcggccgg actggagacc gatccggggc
      721 cgctcgcttt gcgcaaattc ttctggcgcg ggctggacgt gaccatttcg cgtttgaacg
      781 acggttacga actgtggtgc gcgcccgacg atgcgctgat cgtctgggac cgcatcgcca
      841 aggccgggtc tgtcttcgcg ctttgcccgg ctggccagcg cgccctggac atcatcgatc
      901 tggaaagcgg catcgtgcgg cctggccgcg atttcgcgcc tgcccgcgac ggcttttcgc
      961 cggaaccctc gccacagtcg cttggcctga gcggcctcgt cgaccgcgat tgcatcttca
     1021 acggccgtgc cggatatctg gcgggcggtg gggggaaggc cctggccgga atcctgttcg
     1081 actccgagac gccgatgccg caaaccgttc tcatgcgcgg caaccttgcc gttggatcca
     1141 ccctcgactc gctggtctct ccggcgctgc gccgcgccgc cgccttggcc attttggaga
     1201 cggatgcggc cgcgccggga accgcgctga ccgccggggg cgccgcctgc cgcacctgcg
     1261 ccttgccatt tctgccgact ccggctccgc tagccccggc gacggagaat ccgcctcccg
     1321 gcgtttaacg attggagcgg ccggcttgga caccggaatg aaaatcgatc cggatgccga
     1381 gcttgtcgct cgcgctggca aaggcgaccg cgccgccgcg caagccctga tggtgcgcca
     1441 tctgccgaaa atgctgacct tggcccggcg gatgctgcgc gaccggaccg aggccgagga
     1501 cgcggtgcag gaggcgtttc tcaagttgtg gacgcatgcc gcgcgctggc gtccgggaca
     1561 ggcgaaattc gagacctggc tttaccgggt gacgctgaat cagtgctacg accggctccg
     1621 gcgcaagccc acgctcgaac tggaggcggc ggccaaggtt cccgatcctg cgcccgggcc
     1681 ggagacggag cttgagaacg ccggcctggc ggcggaggtc gaggcggcgc tggccgagtt
     1741 gcccgagcgg cagcgcgcgg ccatcctttt gtgccaccat gaagagcgcg gcaacatgga
     1801 agcggcggag attctgggca tcagcgttga agcaatagag tccctcttgg cgcggggacg
     1861 gcggacgtta aggatgaagc tcgcgcatct gaaggacggt taggagaaga agatggcgga
     1921 aaaggacccg atcgatcgcg cctccgctac ggccgaggcg caaagcgacg ccgcattcgc
     1981 cgcgcgcgcg ctgacggaac tgccgagcgt tcccgtaccc ggtacgctgg aagcgcgcat
     2041 cctcgccgat ttcgacgccg tcgcggcaaa gcggaaggct tcgctctggg cccgggtgct
     2101 gcgtccggcg cgcggcctgc gcgacgcgat atggccggat gcgccggcgt ggaagcccgc
     2161 ctcggtgttc gccttgtcgc tgctgatcgg actggcggcg gggacgctgg ttccgtcttc
     2221 ggctttgtcc ggcgccggtt ccgttcagga cgaaaccgcg gtgctcgata ccgttccgaa
     2281 tttggatatc ttcggggact tctaagatga gcaacggatc gccgcagggc aaatcgaacg
     2341 tggcgctgat catatcgctg tgcatcaacc tcgttctggc cggggtgatc gcgatgggcg
     2401 ggtatcgcgc cttccatcac ggcccgatgt tcgggaacac gcccggcttc gagaaaggat
     2461 atggcggcgg acatggacaa aggccaatgg gttcccgcgc gctcatgcac ttgctccccg
     2521 cggaaaccgg caaggtcaag gccgttgccg acgcgcatag agaccgcgtc ttccagctca
     2581 gaagcgaggc gcggcaggcg cgccgggacg tgctgcgcac ctttgcgtct cccgatttca
     2641 gccagcaggc attcgatcgc gcgctggagc gcgtgcgcaa cgccgacgcg gcgctggaag
     2701 ccgaagagct gaaggtcgtg tccgagagcg tcgccgcgct gacgccggac gaacggcgcg
     2761 ccgccgtcga gcgagcgcgg gagcatcgcg gcttcggcat gcaccgcggc ggccgcagcc
     2821 gcgggttcta gagtttgatc cgatttaatt aaatcggatc aaactctaga ttccttgatt
     2881 ttcgcgcaat ctgacggcaa accgcttcac actttgccgg attgcgctct aaatccggta
     2941 actgcttggc ggatgtttgc taacgcggtt atgcggcaac gacggcgagt ttcacgccca
     3001 gcgcgcgcag cacggcgttc acggtttcgt agcgtggg
//
LOCUS       DASWPU010000586         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_109570,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000586 DASWPU010000000
VERSION     DASWPU010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109570"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VGC27_12380"
     CDS             <1..482
                     /locus_tag="VGC27_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY0283407.1"
                     /translation="HPGKGTPTTTIDMRRISDGVKVTERYKTTESMEKAFIDEKTYNY
                     LYQDGEHFVFMDPVHFEQIHVDKDVVGDWAPYLQENMEVQLSLFNGTAVSIVLPQRVT
                     LEVTDTEPVVKGQTASGSFKPAMLSNGVRTMVPTHIVPGTRVVVMTEDGAYVERAKD"
     gene            complement(611..1561)
                     /locus_tag="VGC27_12385"
     CDS             complement(611..1561)
                     /locus_tag="VGC27_12385"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283408.1"
                     /translation="MNLDPKLLIAGLCLLAISFALALILKQWRQWRLKEAVAPRPQAA
                     AGPRLMAAYGAGAAQVQSVGAKTIAVVHDFGHPYGHHSDERKYLSTREAFEILAEIRS
                     VGKDTPIDIVLHTPGGEVFACELIATALRDRPNTTAYVPYCAMSAGTIIALATEKVVM
                     GKYACLGPIDTQFFGFPIESFVRLLKEKPIQNVDDTTVLLGYLAEKELKSARQRACEL
                     LNKHHFGSDDACQLTDFLVAGDLPHTEQIGRARAAELGVNVGKEDCPAAVYKIVEARL
                     ELFKSMDGPAGFGPDMAVKEVPAPQLRKAFDAPPLMWRKP"
     gene            2175..2714
                     /locus_tag="VGC27_12390"
     CDS             2175..2714
                     /locus_tag="VGC27_12390"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEY0283409.1"
                     /translation="MPVPTPKPSLSSSVIGADDAVPGVVTTAQPTFNIIAEVPAPASD
                     TPTFIKSLRHVYCVEFARIRSGIAIFGDAKTWWDHAKGQYARGTYPLPGAVMVFSAVR
                     KMRHGHVAVVKRVVSSREVVIDHANWGRNGDIYLNAPVIDVSEKNDWSKVRVWNTHAG
                     TMGTNVYPIKGFVSRTAWN"
     gene            complement(2926..3297)
                     /locus_tag="VGC27_12395"
     CDS             complement(2926..3297)
                     /locus_tag="VGC27_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283410.1"
                     /translation="MDDTRRQPTPATPPPLPSPDAVLAEQLARCHGAIAGCFEFCGDG
                     NVPVSQQLETLNIASRLMRVSVVLAKALDKSPKEFIHRIIVERPAMIDVTPATAIPPT
                     PAEKVENNLARKVASDEGVTE"
BASE COUNT          713 a         1130 c         1129 g          725 t
ORIGIN      
        1 tccatcccgg caaaggtacg cccaccacga ccatcgacat gcggcgcatc agcgacgggg
       61 tgaaagtaac cgaacgctac aagacgaccg aaagcatgga aaaggccttc atcgacgaga
      121 agacctacaa ctacctttat caggacggcg agcatttcgt cttcatggat ccggtgcatt
      181 tcgagcagat ccatgtcgac aaggacgtgg tcggcgactg ggcgccctat ctgcaggaga
      241 acatggaagt ccaactgtcg ctctttaacg gcacagcggt ctccatcgta ttgccgcagc
      301 gggtgacgct ggaagtgacc gacaccgaac cggtggtcaa aggccagacg gcgtccggct
      361 cgttcaagcc ggcgatgctc tccaacggcg tgcgcaccat ggtgccgacc catatcgtgc
      421 ccggcacgcg cgtggtggtg atgaccgaag acggcgccta tgtggagcgg gcgaaggact
      481 agcgcgcaat ccggtctcgt ggattcgttt cccggcaccc ttcgacaagc tcagggtgaa
      541 ggttgaagcc aatcccctca tgctggtcgg tacgccgcta ccgactatcg aagcgtgagg
      601 atggcgcttg ctacggtttg cgccacatca gcggcggcgc gtcgaacgcc ttgcgcaact
      661 gcggcgcggg aacttctttc acggccatgt ccggaccgaa gccggcgggg ccgtccatgc
      721 tcttgaagag ttccaggcgg gcctcgacga tcttgtagac cgcagccggg caatcttcct
      781 tgccgacatt gacgcccagt tccgccgcgc gggcgcggcc gatctgttcg gtgtgcggca
      841 aatcgccggc gaccaggaaa tccgtcaact gacaggcgtc gtcgctgccg aaatgatgct
      901 tgttgagcag ctcgcaggcc cgctgccgcg cgctcttcaa ttccttctcc gccagatagc
      961 ccagcagcac cgtggtatcg tcgacgttct gaatcggctt ttccttgagc aaccgaacga
     1021 aagactcgat cgggaagccg aagaactgcg tatcgatcgg accgagacag gcatatttgc
     1081 ccatcaccac tttttcggtg gcgagcgcga tgatggtgcc cgccgacatc gcgcaatagg
     1141 gaacataggc agtcgtgttc ggacggtccc tgagcgcggt ggcgatcaac tcgcaggcga
     1201 acacttcgcc gcccggcgtg tgcaggacga tgtcgatggg cgtgtctttg ccgaccgagc
     1261 ggatctccgc cagaatctcg aaggcttcgc gggtggagag atatttgcgc tcgtcgctgt
     1321 ggtggccgta gggatggccg aaatcgtgaa cgacggcgat agtcttggcg ccgacgctct
     1381 gaacctgcgc ggcgccggcg ccgtaagcgg ccatcagcct gggaccggcc gcggcttgcg
     1441 gacgcggcgc caccgcttcc ttcagccgcc actgccgcca ctgtttcagg atcaaggcca
     1501 gcgcgaacga aatcgccagc agacaaagcc cggctatcag caatttcgga tcgagattca
     1561 caacgccccc acatcccgcc tgtcgcggcc gatttccggt tgccgccgag acacttttct
     1621 tgtcttatca taaccgcagg acggcccatc cgaaagtggg cgaaccgccg tcgatttgcg
     1681 gcgccggatg cgcattgtgc gaagcagcaa gcaagtatcc acacctaaat aatgtagctg
     1741 cgatcttttg tcctgcggcg agagatcggc gcgcgcgaac caagcgggtg gaaatttact
     1801 tttacaatcc gtcatgtgtt ttcccccgaa aacgcctagg gcgagcatct gcttggaggt
     1861 cgattcgggg gcaaaattcg gcgcgaagct ccttcccggc ggctcagtgc gcctgtagcg
     1921 ataattgcgc tgcatcttaa tcgcggacca gcacgatcat tattttggtc cccgccgaat
     1981 caattttcga atcacttatt gtggcctcca cacgacaatg tgggattctc gcaactgttg
     2041 gcatcatact tttgctgagc taaacggttt gcatgattca tcttgactgt gcccgtcgcg
     2101 tcgcagcgat cccgcttgca gccatcttga tcctctccgc cggtggagtt gcatacgcac
     2161 gcaaaaacgt gcccgtgccg gtgcccacac ccaaaccttc cctgtcgtcg agcgtcatcg
     2221 gcgccgatga cgcagtgccg ggcgtcgtta ccaccgcgca gccgaccttc aacatcatcg
     2281 ccgaagtgcc ggcgccggcg agcgacacgc cgaccttcat caaatccctg cgccatgtct
     2341 attgcgtgga attcgcccgc atccgttccg gcatcgcgat cttcggcgac gccaagacct
     2401 ggtgggatca cgccaaaggc caatatgcca gaggcaccta cccgcttccc ggcgccgtga
     2461 tggtgttctc cgcggtcagg aaaatgcggc acggccatgt ggccgtggtg aagcgcgtcg
     2521 tctccagccg cgaagtcgtt atcgatcacg ccaattgggg gcgcaacggc gacatctatc
     2581 tcaacgcgcc ggtgatcgac gtcagcgaga aaaacgattg gtccaaggtg cgggtctgga
     2641 atacgcacgc cggaacgatg ggcaccaacg tctatccgat caagggcttc gtttcccgca
     2701 cggcgtggaa ctgaacaaga gtgaacaggg cgcggcgaag tgcgaatagg gattctgttc
     2761 gcagcgcgcc tttcgtcgac ggccgccagt gtccggcgca ccgagcggca caatgcccaa
     2821 gcccgcgccc tgagcgcccg catctcgcgc gtgtgacggc cggtcttcag cgcattgcga
     2881 ttgccgggcg gcgcgccgcc ttggttctta cgacgcaaac ccggcctatt cggtaacgcc
     2941 ttcatccgac gccactttcc ttgcgaggtt gttttcgact ttttcagcgg gggtgggggg
     3001 tattgccgtt gccggcgtga cgtcgatcat cgccggacgc tccacgatga tgcgatggat
     3061 gaactctttg ggcgatttgt cgagggcttt cgcaagcacg acgctgacgc gcatcagccg
     3121 cgaggcgata ttcagcgttt cgagttgctg agagaccgga acattgccgt cgccgcagaa
     3181 ctcaaagcat cccgcgatcg cgccgtggca tcgcgccaat tgctcggcga gcacggcgtc
     3241 gggactgggc agcggcggcg gggttgcagg ggtgggttgt cggcgggtgt catccatggc
     3301 ggttctccgg actttatcag ccgcgcatta tcaggccgga ttcgcgggct tggataagtt
     3361 cgacaccgtg gcgggttgat gaatgagatc aaaggcttca ggcggaaggc agacgggata
     3421 tcttccgctg cttacgagca gggcccctta tgtgtcctca cctggatggc ccgctcttgc
     3481 gggccatgac aagaaggaac aaacgatttc gcccagttcg gaccggtgca acaaaacaaa
     3541 agccccacac ggtttcccgt gcggggcttt ggcgtgatgg cggggctcgc gcgcgttggc
     3601 gcgcggatca aataactcat tctgtccttg gcaggactgg cagcgaccga ctctcccgcg
     3661 gcttgggccg cagtaccatg ggcgctggag gctttaa
//
LOCUS       DASWPU010000587        17294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_10641,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000587 DASWPU010000000
VERSION     DASWPU010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 17294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17294
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10641"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(597..1835)
                     /locus_tag="VGC27_12400"
     CDS             complement(597..1835)
                     /locus_tag="VGC27_12400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEY0283411.1"
                     /translation="MAIEIGAGTDGGLPSARSLRGPLAFAISCFLVWGLAYGLLDVLN
                     KHFQETLHVSKAQSSLLQIAYFGAYLLMSIPAGILMQARGYKFGLVTGLLVTAFGAFL
                     FVPSAQVESFTFFVGSMFVLATGLCILETSADTYVSVLGKPEDAPKRLNLAQSFNSLG
                     VFFGPIIGGSVFFSPTVTEALGGAQHSVQLTYLVIGIAVVLFAFVMSRAWLPEIKLED
                     HAPAGTVQTPSKPLIRQRHFVFGFITQFIYIGAQVGIFAFFLNQATETWQGMTTQSAA
                     FLFSIGVLAYLVGRFATTALMLYVQPRIILTIYGVANVILCLIAAAGIQKVSALALIP
                     VFFFMGTMFPTIFTLGIRDLGDKTKRASSIMVMAIGGGIALPYPMGLIGDAYGMPVAF
                     LLPAVGFALVALYGWIGSRK"
     gene            complement(1934..2989)
                     /locus_tag="VGC27_12405"
     CDS             complement(1934..2989)
                     /locus_tag="VGC27_12405"
                     /inference="COORDINATES: protein motif:HMM:NF044822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283412.1"
                     /translation="MPGGLNVGMQLFWLETAVPEKARRGRIVNWPQYWAWRLSGVAAT
                     EVTSLGCHTDLWIPAEGKPSPLAVARGWAERMAPLRRASDVLGPVTEEWRTRCNLPHD
                     CMVLCGMHDSNAALLATRGYPEIGDSECTIMSTGTWFIAMRALSAQTKMASISLPEAR
                     DCLVNVDVSGTPVPSSRFMGGREMEIMAPEALSCVDLLADKDALVSTVKSLVSEGTFA
                     LPTFQKGVGPFPRSAGHWIRQPTDQLGCRAAAGLYLALMANVSLDLIGSKEALVIEGR
                     FANDPVFTGTLASLRRNQQISLSDANNNLPYGALRLLDDELPPQAKLVRVEPLDHDLS
                     DYAAEWQSMAQNAEAAA"
     gene            complement(3504..4259)
                     /locus_tag="VGC27_12410"
     CDS             complement(3504..4259)
                     /locus_tag="VGC27_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0283413.1"
                     /translation="MPNSIAEDLLTLSHEIGREDRGLALLGEGNTSARLGEDSYLIKA
                     SGSCLGTLRPEDVVECRMSLVLGMLEEDRLTDQQVDDALLASRLDQKAKKPSVEAVFH
                     AWALTLPGIKFVGHCHAIAVNQILCSPRARDFAEHRMFPDEIVCCGVASVFIPPVDPG
                     LKLAQAIRRETKAFIEKHQHTPRIILMQNHGIIALGATWQSVLAGLLMAEKAAKVFIG
                     AAQLGGPIFLSEKDIARIVGRPDEAYRQRVLKL"
     gene            complement(4265..5077)
                     /locus_tag="VGC27_12415"
     CDS             complement(4265..5077)
                     /locus_tag="VGC27_12415"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0283414.1"
                     /translation="MSANLADKIAIVTGGAQGLGRAICQRLVREGCVVVVADVNEKGV
                     EDTAASIAAEPGCRIVGMKVDVTQEADVKALFDRAVREFGRVDIVVSNAAILIAEPII
                     DADAEKWRAVMNVNLFGYFLVTKHACRVMKQQRGGSIININSKSGKKGSAANSAYAAS
                     KFGGIGHTQSVALEMAPFNVRCNAVCPGNLLDSPLWTDPEKGLFVQYLRAGKVPGAKS
                     VEDVREKYVNQVPMKRGCSYDDVCNAVVFLASEQSSYITGIALSVTGGQEMS"
     gene            complement(5074..6648)
                     /locus_tag="VGC27_12420"
     CDS             complement(5074..6648)
                     /locus_tag="VGC27_12420"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0283415.1"
                     /translation="MQLVGPDQLQVNEDKTVFEPGPSQILGRVEVVGLCFSDLKLLKQ
                     FSGHARKSQVTAGIDAAALSQMPHYVPGDKPAVPGHETVVRIVKAGPGVTRHKVGDRF
                     LVQTDYRWLPTVSSNAAFGYNFEGALQEYVLMDERVIVSPEGESMLIPVPEDLSASAL
                     ALVEPWACVEDSYVEKQRQTLKDGGRMLVVGEAQVDESRVASLPGKPGIATFITADKI
                     ADLKNAAYDDVIYFGSNPETAERLFPKVAADGLFNIVQDGGKFGRAVTSQVGRVHYGG
                     IRVIGTTGSDPAAAMAAIPATTEIRQNDKINVIGAGGPMGTMHVIRDLCQGVPGVTVF
                     AGDLSDERLAALRELAEPLALKNHVTLRPYNPSKDKLAEKFDYVVLMAPVPALVTQAV
                     SAAAPRAIINIFAGIPAEITADLDFDAYIEKQLYFVGTSGSVLDDMKQVLAKVVSRRL
                     DTNLSVAAVSGLDGAIEGIRAVEKNLLAGKILIYPSCRGLKLTPLAELGAKVPLENGH
                     WSRQAEDALLKQYPRT"
     gene            complement(6691..7974)
                     /locus_tag="VGC27_12425"
     CDS             complement(6691..7974)
                     /locus_tag="VGC27_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283416.1"
                     /translation="MERQLDSFDPQQRRSALNSLWRLAQEGEVVLPEAGTDINLHAHT
                     FFSYNAYGYSPSKFAWLARKAGLAMAGIVDFDVLDALDEFLEAGRLIGLKTCVSLESR
                     VFMPEFNSRVINSPGEPGVAYHMGVGFSKVVQHPYVAEMRAAAARRTRDIVDRVNLYL
                     RPVELDFEKDVLPLTPKGNATERHLCEAYERKAALLFPDAAQRAAFWMEKIGDAPHAG
                     IKLQNLIRLKLMKKGGVGYVQPEAGSFPLMADMNRFVLESGAIPTLAWLDGTSEGEQW
                     IEEFIHVGMTTGAAALNIIPDRNYTPGVKDQKLRNLYDIVALAQKYDFPVIVGTEMNS
                     PGHKFVDSFETEELKPLVPVFLRGAHIVYAHSVLQRQSGMGYLSPWASKSFATIAAKN
                     EFFEELGRELQPATEERLCALTASATPEQILAKLH"
     gene            8348..10648
                     /locus_tag="VGC27_12430"
     CDS             8348..10648
                     /locus_tag="VGC27_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HEY0283417.1"
                     /translation="MPKSPVHLSDSRPSFAPFEIDCGKIPAYRYKGNLQSELDAKTLA
                     PAEAVAILEDMLVIRELEEMIVKLRSGAYEPIRDFNYRGPTHVSVGQEGTAAGACCAL
                     QLPDNITSTHRGHGESLAKGTVAIRQMTDEQLRKRVPNCKSDKHDDLIEAALEEHVYR
                     TICELFGKDDGYCRGRGGSMHIADFSIGHLGANAIVGGGVPIATGAALANRYFERGNV
                     VCCFAGDGAYANGVVLESLNFAAQAQFTNHYAGGNGYGLPIIFLVCNNHYGMTHRTDD
                     EVMGVDRMARRPAGFADNNMHSEIVNGMDVLAVRDAVLRAAKLCREGKGPVFLDVDCY
                     RYWGHSLSDPRNEYRTKEEEAAWKAVDAVESYKKKLLATRVLDENGIRAVEKRVADRN
                     AVAAKRAAAAADPDAKDVLTFMYTDTACETVPAEFAKVELFGPLPEIKRVNGELSYKD
                     ALKEALVQEMARDNRVVFYGEDVADYGGAFKVTKGLLEAFGRDRVFNSPISEACICGT
                     GCGAAMNGLRPIVELMYFDFALMSSDQISNQAAKWHYMSGAQTEVPLVIRASAGAGKG
                     YGGQHSQTLESMFCHIPGLYVVYPATPADAKGMLKSAIRDNNPILFVESQALYGMKGP
                     VPEGEYLVPLGVAEVKREGSDITLVAWGPLVHDCFTAAEKLKAEKNVSAEVIDLRSLV
                     PLDIETVLRSVRKTGRCVVASQAIHIGSYTGEIASTIQDLAFDYLDAPVKRVGAKNGI
                     APQSHILEAAFLPNVDDILAAANAIL"
     gene            10657..12009
                     /locus_tag="VGC27_12435"
     CDS             10657..12009
                     /locus_tag="VGC27_12435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY0283418.1"
                     /translation="MATPIIMPKFGQMTEESAIIEWLKKEGDAVAKGDILFTVETDKS
                     VMEVESFEEGTLLKIAVKPGISVPVQSVVGFLGKPGEPVPEAAPPVAPAPVKTEPPKA
                     AVRVAPVAASSPAIPVAAPAAVASPVVVAAPVEPALFRISPRAAALARDCVIDPAGIK
                     GSGPGGRVVEKDVKAYLEAKGYYRLRVSPLAKQLAAKESLDLLTIHSADASGRIGVVD
                     VERAVAEKPKPMSQMRQIIAQRLTQSVVTAPHFYVTAEVDMTDLVGFRAQLKAKGAPY
                     TVTDFISLAAVLALKDFPNVNSSTDGRTVRWNSHVHLGIAVSLEQGLVVPVIRNADEL
                     TMAELNTRAKELTEKARAGKLTPDEMAGGTFTISNMGMLDVENFTAIINPGESAILAV
                     SSVLEKPVVRDGKVVIRSIMKMTLSSDHRLIDGAMAARFVNALKKKIEDVELWRSLVP
                     "
     gene            12069..13367
                     /locus_tag="VGC27_12440"
     CDS             12069..13367
                     /locus_tag="VGC27_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY0283419.1"
                     /translation="MAKIQEPLAQETIESHNKRHLGAIREDYDALGRTLARRKIDIDA
                     VKRRVAGFSVAVPSWGAGVGGTRFARFPIPGEPTNVHEKLVDCAVIQQLCRMTPRVST
                     HFPWDYVDDPDGLRQEAEQLGLSFDAVNSNTFEDQPGQPLSYRYGSLSATDKAVREQA
                     VKHNIDCIRIGRKLGSKALTVWVGDGTNFPGQQSLRDSLDRYLEGAHAIYAELPADWR
                     MFIEHKLYEPAFYSTVISDWGTNIMVAKELGPKAKCLVDLGHHAPNVNIEQIVARLSR
                     FGKLGGFHFNDSKYGDDDLDTGSINPHQLFLVFNELVEAERAAPKDFDPAYMIDQSHN
                     ITDPIESLLASAEAIAGAFARAQIVDRDTLATCREQNDVMEAFRTLRHAYEVDVAPIL
                     ALVRVEAGGAADPILAYRESAWRARKAQERSPSKLGSGIV"
     gene            13451..14233
                     /locus_tag="VGC27_12445"
                     /pseudo
     CDS             13451..14233
                     /locus_tag="VGC27_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
     gene            complement(14328..15092)
                     /locus_tag="VGC27_12450"
     CDS             complement(14328..15092)
                     /locus_tag="VGC27_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283420.1"
                     /translation="MTITNCHVSGYDVGSLLDGTYKRSQLKNIEPHLTSGRIKLGTES
                     NGGFRNIAISNCVFDCGNGLAIESVDGGVLEDVVVNNIVMRDTTAAPIFLRLGDRRRA
                     PAGTPIAALRRVTISDIDAYCLDKRYCASITAVPGAMVEDVTLSGIRLIYPGGGTAED
                     AAIKLPEVPEKYPDPDMFGVMPAWGLYMRHVRGIILRDVELTSVAADARPPVVAEDVS
                     GLITDALLAASGPGKPLQPVLMSKPVKQRKHRRNEN"
     gene            complement(15096..15893)
                     /locus_tag="VGC27_12455"
     CDS             complement(15096..15893)
                     /locus_tag="VGC27_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEY0283421.1"
                     /translation="MAFLPAFASSAFARPIDVFDVRDYGAKGNDTALDTAAIQRAIDA
                     ASAAGGGTVYLPTGRYLSFTIRLKSHITLLFASGAVLIAADPAKHGGSYDLPEANPWD
                     AYQDYGHSYFRNSLIWGEDLEDIAIMGPGLIDGDGLTRESPNAPWSIGVGRDWPASPE
                     LDAQLASHNAEVKAMSGKGNKAIALKNSRNVTIRDVSILRGGHFAILATGVDNLTIDN
                     MRIDTNRDGIDLDVVRNARLSNLSVNSPTDDAIVLKSSLALGTARPT"
     gene            16141..>17294
                     /locus_tag="VGC27_12460"
     CDS             16141..>17294
                     /locus_tag="VGC27_12460"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY0283422.1"
                     /translation="MKKLPGIFSRQKRLIAPRLWKVLPLLLFSSLAMAGPSLAAEPTR
                     HHVAKASRDILVNGFRTPPQAAKPRVWWHWMNGNITKDGIRKDIEWMKRIGIGGMDAI
                     DATIETPQVVKHRLIYMTPEWKDAFRYSTELADKYGMELAINSSPGWSMTGGPWVTPQ
                     QAMKKLVWSETEVQGGKPFHGVLPKPPDNPGPFQNIKNAPGYDQFSPAAASFYRDSIV
                     VAYRAPVGKPVVSEAVSDSGAIDAAVLSDGDLTNGVTLIPKEADKDVWIRLAYKDPVR
                     IQGLTLAISTPDGLGFAAAVEVSDDGQSWRHVADVPQRQQIRRFALLQQTISFPPVTG
                     RFFRVVLRPHAPIPTSLRIVFNAPGVVVNTRPTPDKPSYRVYELAFHAGAT"
BASE COUNT         3479 a         5192 c         5246 g         3377 t
ORIGIN      
        1 cggtgctcac aaaatcaggg cgaaatggcg tttcgccatg agcagtgatg atgagtccgg
       61 cagaggaaat gtcaacggca ccaagggttt gtgagaagct atgttgcgct atgagaatct
      121 gaaatatttt tctcttaatc agcgggtcgc aggttcgagt cctgcatcac ccaccattca
      181 gtccggcccc agcctgtgtt ttttaggggc gttcggccgt ctcagacccg cgagcgtttg
      241 tccttggaca tgggaccgga gtgatcagat cggcgcgcgt gtcgcacctg caagcttcgg
      301 tttgtccggc ctcgagaatg tgttgtggca agggcgggcc ttgatcttca cgatccgcgg
      361 ggcagttcta gagtgtgatc cgatttaatt gaatcggatc acactctcga tttcttgttt
      421 ttcgcgcaat ctgacggcaa accgcgtcgg cacgctgctg ctaccgacca ctttgccgga
      481 ttgcgctcta gaaattgcgg acttcaaaga tggggagcgg caggttcagc cgctccctct
      541 tttttaagcg aggctttgac gagcggatgc cgggagttcg ccggtcagcc cctccctcac
      601 ttccttgaac cgatccagcc atacagcgcg accagcgcga agcccaccgc cggcagcagg
      661 aaggcaaccg gcatgccgta ggcgtcgccg atcagcccca tcggataagg cagggcgatg
      721 ccgccgccaa tagccatgac catgatcgac gaggcgcgtt tggtcttgtc gcccagatcg
      781 cggatgccca gcgtgaagat ggtcggaaac atcgttccca tgaagaagaa gaccgggatg
      841 agcgccagtg ccgagacctt ctggatgccg gcggcggcga tcaggcagag gatcacgttc
      901 gccacgccgt agatcgtgag aatgatccgc ggctggacgt acagcatcag cgcggtggtc
      961 gcgaaacggc ccacaagata agccagcacg ccgatggaga agaggaacgc ggcggactgc
     1021 gtggtcatgc cctgccaggt ctcggtcgcc tgattcagga agaacgcaaa gatgcccacc
     1081 tgcgcgccga tatagatgaa ctgcgtgatg aagccgaaca cgaagtgccg ctggcggatc
     1141 agcggtttcg acggggtttg cacggttccg gccggagcat ggtcctccag cttgatctcg
     1201 ggcagccagg ccctcgacat cacgaaggcg aacagcacca cggcgatgcc gatgacgaga
     1261 taggtgagct gcaccgagtg ttgtgccccg cccagcgcct cggttacggt ggggctgaag
     1321 aacaccgaac cgccgatgat gggtccgaag aagacgccga gagaattgaa cgactgcgcc
     1381 agattaagcc gcttgggcgc atcctccggc ttgccgagca cgctgacata ggtgtcggcc
     1441 gaggtctcca ggatgcacaa gcccgtcgcc agcacgaaca tcgagccgac gaagaaggtg
     1501 aagctttcga cctgggcaga gggcacgaaa aggaaagcgc cgaaggccgt gaccagcaat
     1561 cccgtcacca gcccgaactt atagcccctg gcctgcatca ggatgccggc cggaatgctc
     1621 atcaggagat aggcgccgaa ataagcgatc tgaagcaagg acgactgcgc cttgctgacg
     1681 tgcagcgttt cttggaaatg cttgttcagc acgtccaaga gaccgtaggc cagtccccaa
     1741 accaggaaac aactgattgc gaaagccagc ggaccgcgta gagaccgcgc ggagggcaaa
     1801 ccgccgtccg ttcccgcacc gatttcaatc gccatgtttc tccccttgag acgttcttta
     1861 tggttttcca gtttttcaca tcaacgcgaa agccggttat gcgttttgca acagccttgg
     1921 cctggaactg cgatcatgcc gccgcctcgg cattctgcgc catcgattgc cattcagccg
     1981 cgtaatccga caaatcgtga tcgagcggtt ccacgcgtac aagcttggct tgcggaggaa
     2041 gctcgtcgtc gaggagccgc agcgcgccgt agggcagatt gttgttggca tctgacaggc
     2101 tgatttgttg attgcggcgc agcgacgcca gcgttccggt gaacaccgga tcgttggcaa
     2161 aacgcccctc gatcacgagg gcttccttgg agccgatcaa atcgagagaa acattcgcca
     2221 tcaaggcgag ataaagcccg gccgcggcgc ggcagccgag ttggtcggtc ggctgacgga
     2281 tccaatgacc ggcgcttctt gggaatggcc ccacgccttt ttggaacgtc ggcagtgcga
     2341 acgtgccttc actgaccaag ctcttgacag tgctgaccag ggcatcttta tcggccagaa
     2401 gatcgacaca tgacaacgct tccggcgcca tgatctccat ctcccgcccg cccatgaacc
     2461 gcgaagaagg aaccggcgtc cccgatacat caacgttgac caggcagtct cgcgcttcgg
     2521 gaagcgaaat cgaggccatc tttgtctggg cggagagggc gcgcatcgcg atgaaccaag
     2581 tcccggtgga cataatcgtg cattcgctgt cgccgatctc cggatagccg cgcgttgcca
     2641 gcaacgcggc attgctgtcg tgcatcccgc aaaggaccat gcagtcatgc ggcagattgc
     2701 agcgggtgcg ccactcttcc gtgacgggac cgagcacatc gctggcacgc cgcaagggtg
     2761 ccatccgttc ggcccagccg cgcgccaccg ccaaaggaga cggctttccc tcggcgggta
     2821 tccagagatc ggtgtggcag ccgaggctgg tgacttccgt tgcggcgacg ccgctcaatc
     2881 gccaagccca atattgcggc caattgacga tgcgccctcg ccgggccttc tctggaacag
     2941 cggtttcgag ccagaacagt tgcatgccca cattgaggcc gcccggcaat gaaggcgaac
     3001 ccgtcagcga gaacggatcg cgcaggcgga gatattgttc cttcacctca ggcggcggct
     3061 cggcttcgta gtccagcggc ggaaggcaca gaccatcgtc gtccataagg cataccgagg
     3121 cgccgtgagc gacgggaacg attgccgcga tctttcctag gcgggcgaat atggcgagcg
     3181 tttgggccaa ccagaggttg atacccttaa cgtcaagtgt cgggtagccg tcgcacaagg
     3241 catacgtgtt acgatgagtt cggcgctcca caagctgctt gtctggcgac caaatcgtca
     3301 gctttgacag cgtcttgccg atatccagga caacgacgca accgggcagc aatttcctca
     3361 cccttatcta ttttgactga ttttgaacaa atatgattgg attagtcaag gtaacgtaga
     3421 accgcagcct aatacggtat tcggccgctg gcacggctta taatcgttcg caagtagcgc
     3481 tggcgccttg gcggcaggcg ggatcacagc ttcagtacgc gctgccggta ggcttcgtcg
     3541 gggcggccaa cgatgcgcgc gatatccttt tcggagagga agatcgggcc gccaagctgc
     3601 gctgcgccga tgaagacctt ggccgccttc tccgccatca gcaggccggc gagaacggat
     3661 tgccaggttg ctcccagcgc gatgatgcca tggttctgca tcagaatgat tcgcggcgtg
     3721 tgctgatgct tttcgatgaa ggctttggtc tcgcggcgga tggcttgcgc cagcttcagg
     3781 cccggatcga cgggcgggat gaacaccgaa gcgacgccgc aacagacgat ttcgtctggg
     3841 aacatccgat gttcggcgaa gtccctagcg cgcggcgagc acaagatctg attgacagct
     3901 atggcgtggc aatgtccgac gaatttgatg ccgggcaggg taagcgccca ggcatggaac
     3961 accgcttcga cggacggctt tttggccttt tgatccaatc gggaagcaag cagggcgtcg
     4021 tccacctgct gatcggtcag gcggtcttct tccagcatcc ccaacaccag ggacatgcgg
     4081 cattcgacga catcctctgg ccgcaaggtg ccgaggcaac tgccgctggc tttgatgaga
     4141 tacgaatctt cgccgagccg ggcggaggtg ttgccttccc caagcaaggc gaggccgcga
     4201 tcctcgcggc cgatttcgtg ggaaagggtc aacaggtctt cggcgatcga atttggcatg
     4261 atggtcaact catttcctgg ccgccggtca cgctcagcgc aatgccggtg atataagacg
     4321 attgctcgga tgcgagaaac acgaccgcat tgcagacgtc gtcgtaacta cagccgcgtt
     4381 tcatcggtac ctgattgacg tatttctcgc gtacatcttc gacgctcttc gcgccgggaa
     4441 ccttaccagc gcggaggtac tgcacaaaca gtcccttttc gggatccgtc cacagcggcg
     4501 aatcgagcag gttgccggga cagactgcgt tgcagcggac gttgaagggc gccatttcaa
     4561 gcgcgacgct ttgagtgtgc ccgatgccgc cgaacttgct cgcggcatag gcggaattgg
     4621 cggcgctgcc ctttttaccg gacttggaat tgatgttgat gatcgagccg ccgcgctgct
     4681 gcttcatgac gcggcaggcg tgcttcgtta ccaggaaata gccgaacagg ttcacgttca
     4741 tcaccgcgcg ccacttctcg gcgtcggcgt cgatgatcgg ttcggcgatc aggatcgccg
     4801 cattggagac gacaatatcc acgcggccga attcccgcac ggcccggtcg aacagcgcct
     4861 ttacgtcggc ttcctgcgtc acatccactt tcatgccgac gatgcggcag ccgggttccg
     4921 cggcgatgga cgcggccgta tcctcgaccc ccttctcgtt tacgtcggcg acgaccacga
     4981 cgcacccttc gcggaccagt ctttgacaga tcgcgcggcc gagtccctgt gcgccgccgg
     5041 tgacgatcgc gattttgtca gctaggtttg cgctcatgtt cttgggtact gcttcaacaa
     5101 tgcgtcctcg gcctgccggc tccagtgacc gttctcaagg ggaaccttgg ccccaagttc
     5161 cgccaagggg gtcagcttca gtcctcggca ggacggatag atgagaatct tgcccgccag
     5221 caggtttttt tcgaccgcgc gtatgccttc gatggcgccg tccaatccgg acacggcggc
     5281 aaccgaaaga ttcgtatcca gacggcgcga aacgaccttg gccagaacct gtttcatgtc
     5341 gtcgagaacc gacccgctgg tgccaacgaa gtaaagctgc ttttcgatgt aggcatcgaa
     5401 gtcgaggtcg gcggtaatct ctgccggaat gccggcgaag atgttgatga tggcgcgcgg
     5461 ggcggctgcg gacacggctt gcgtcaccag cgccggcacc ggcgccatca gcacgacgta
     5521 atcgaacttc tcagcgagct tatccttgga tggattgtac gggcgcagcg tgacgtgatt
     5581 cttcagggcc aggggttcgg ccaactcccg aagtgccgcc aatcgttcgt cgctgagatc
     5641 gcccgcgaac accgttactc caggcacgcc ctggcagaga tcgcggatga catgcatcgt
     5701 ccccatcggg ccgcccgctc cgatgacgtt gattttgtcg ttctgccgga tttccgtcgt
     5761 ggcgggaata gccgccatcg ccgccgccgg gtcggacccg gtcgttccga tgacgcggat
     5821 gccgccataa tggacgcggc ccacctgcga agtgaccgcg cggccgaact tgccgccgtc
     5881 ctgaacgatg ttgaacaaac cgtcggccgc gactttggga aaaagcctct ccgccgtttc
     5941 gggattcgag ccgaagtaga tgacgtcgtc gtaggcggcg ttcttcagat cggcgatctt
     6001 gtcggccgtg atgaacgtcg caattcccgg cttgcccggc aggctcgcca cgcgactctc
     6061 gtctacttgc gcttcgccga caacgagcat tcggccgccg tccttgaggg tctgccgctg
     6121 tttttcgacg taggaatcct cgacgcacgc ccacggctcg accagcgcca gcgcggatgc
     6181 cgacaggtct tccggcacgg ggatgagcat ggattcgccc tcgggtgaaa cgataacgcg
     6241 ctcgtccatc agcacgtatt cctgcaacgc gccttcgaaa ttgtagccga acgccgcgtt
     6301 ggagctgacc gtcggcagcc agcggtaatc ggtctggacc agaaagcggt cgccgacttt
     6361 atgacgggtg actccggggc cggccttgac gatgcgcacg acggtctcgt gaccgggcac
     6421 cgccggtttg tcgccgggca catagtgagg catctgcgac aaagctgctg cgtcaatgcc
     6481 ggccgtgacc tgcgatttgc gggcgtggcc ggaaaactgt ttgagcagct tcagatcgga
     6541 gaagcacagg ccgacgactt cgacccttcc cagaatctgg ctaggccccg gctcgaagac
     6601 cgtcttgtct tcgttgacct gcaactgatc tggtccgacc aattgcaccg cacgctgctt
     6661 acatggaacg tttgtcgttg tcatatccga tcaatgtagc ttcgccaaaa tctgctccgg
     6721 cgtcgcactc gcggtcaggg cgcacaatct ttcttcggtc gcgggctgga gctcgcgccc
     6781 cagttcttca aagaattcgt ttttcgccgc gatcgtcgca aacgacttgc ttgcccaggg
     6841 actgagatag cccatcccgc tttggcgctg caacaccgaa tgggcgtaga cgatgtgcgc
     6901 gccccgcagg aacaccggaa ccagcggctt gagttcctcg gtttcgaacg agtccacgaa
     6961 tttgtggccc ggcgagttca tctcggtgcc gacgatgacg ggaaaatcgt atttctgcgc
     7021 cagagccacg atgtcgtaaa ggtttcgcag tttctggtcc ttgacgcccg gcgtgtagtt
     7081 gcggtcggga atgatgttca gcgccgccgc gcccgtcgtc atgccgacat ggatgaattc
     7141 ctcgatccac tgttcgcctt cgctcgtgcc gtcaagccac gccagcgtcg gaatcgcgcc
     7201 cgattccaga acgaaacggt tcatgtcggc catcagaggg aacgaacccg cttcgggctg
     7261 cacataaccg acgccgcctt tcttcatcaa tttgaggcgg atcaggtttt gaagcttgat
     7321 cccggcgtgc ggggcgtcgc cgattttctc catccagaaa gcggcgcgct gcgccgcgtc
     7381 cggaaatagc aaggcggctt tgcgctcgta ggcttcgcag aggtggcgct cggtcgcgtt
     7441 acctttgggc gttaatggca gcacatcttt ctcgaagtcg agttcgacgg gccgcaggta
     7501 gaggttgacg cggtcgacga tgtcgcgcgt gcgccgcgcc gctgccgccc gcatttcggc
     7561 cacatacgga tgctgaacga ctttcgaaaa cccgacgccc atatgatagg cgacacccgg
     7621 ttcgccgggc gagttgatca cgcgcgagtt gaattccggc atgaataccc gcgattcgag
     7681 gctgacacag gtcttcagtc cgatgaggcg tcccgcctcg aggaattcgt ccaaggcatc
     7741 cagcacgtcg aaatccacga ttccggccat ggccaatccc gccttgcggg ccagccaggc
     7801 gaatttcgat ggtgaatagc cgtaggcgtt gtaggaaaag aaggtatgcg cgtgaaggtt
     7861 tatgtcggtg cccgcctcgg gcaacacgac ttctccctct tgggcgagcc gccacaagga
     7921 attgagggct gaccggcgct gctggggatc aaaactgtcg agttgacgtt ccaacgattc
     7981 cgtcgcggaa ttcggcttac cgaaagtgcg aggaacatgt accagcatca caagaaaact
     8041 ccgaatagat tcaatgctgg agggcctaaa cgcggtactc caaacgccac tcggccggcg
     8101 accgattcat cagccatgac agcgctttct aagaatcggg gagaccgatg caaccatgcc
     8161 gcacttgccg ccccttattt attttgactg attttgcacg aatatgattg aattagtcaa
     8221 gacaacaacg aattgtagcc cggttctgac gccgaaatag gcaatttgtc ctagctccct
     8281 aggggctcca ttgcggcgat gccgacactg cagcacacaa agaggatgga agtggagaat
     8341 agaaggcatg cctaaatcac ctgtccattt gagcgattct cggccgtcat ttgcaccgtt
     8401 cgaaatcgat tgcggcaaga ttcccgccta tcgctacaag ggcaatctcc aatccgaact
     8461 tgatgccaag acgctcgccc ccgccgaagc cgtcgcgatc ctggaagaca tgctcgtcat
     8521 ccgcgaactc gaagagatga tcgttaagct gcggtccggc gcttacgaac cgattcgcga
     8581 ttttaactat cgcggtccga cgcacgtttc ggtcggccag gaaggcaccg ccgccggcgc
     8641 ctgctgtgcg cttcaactgc ccgacaacat caccagcact catcgcggcc acggcgaaag
     8701 tttggcaaag ggcaccgtcg ccatccgcca gatgaccgac gaacaacttc gcaaacgcgt
     8761 gccgaactgc aagtccgata agcacgacga tctgatcgag gcggcgctgg aagagcatgt
     8821 ttaccgcacc atctgcgaat tgttcggcaa ggacgacggc tattgccgcg gccgcggcgg
     8881 ttcgatgcac attgccgact tttccatcgg tcatctcggc gccaacgcca tcgtcggcgg
     8941 tggcgtgccg atcgccacgg gtgccgccct ggccaaccgc tactttgagc gcggcaacgt
     9001 cgtctgctgc tttgccggcg acggcgccta tgccaatggc gtcgtgctgg aatcgttgaa
     9061 cttcgccgcc caggcgcagt tcaccaacca ttacgccggc ggcaacgggt acggcctgcc
     9121 gataatcttc ctggtctgca acaaccatta cggcatgacg catcgcaccg acgatgaagt
     9181 catgggcgtg gaccgtatgg cgcgccgccc ggccggcttc gccgacaaca acatgcattc
     9241 ggaaatcgtc aacggcatgg atgtgcttgc cgtccgcgat gcggtgttgc gcgccgccaa
     9301 gctctgccgc gagggtaaag gcccggtgtt cctcgacgtg gattgctatc gttattgggg
     9361 ccactcgctc agcgatccgc gcaacgagta ccgcaccaag gaagaggaag cggcctggaa
     9421 ggcggtcgac gcggtcgaat cgtacaagaa gaagctgctc gctacccggg tgctcgacga
     9481 gaacggcatc cgcgcggtgg aaaaacgcgt ggccgaccgc aatgccgtcg ccgccaagcg
     9541 cgccgcggcc gccgcggatc ccgacgccaa agacgtgctg actttcatgt acaccgatac
     9601 ggcgtgcgag actgttcccg ccgagttcgc aaaggtggaa ctgttcggcc cgctgccgga
     9661 aatcaagcgc gtcaacggcg agctgagcta caaggacgcg ctcaaggaag cgctcgtcca
     9721 ggaaatggcg cgcgacaatc gcgtggtgtt ttacggcgaa gacgtggccg attacggcgg
     9781 cgccttcaaa gttaccaagg gattgctcga agcgttcggg cgcgaccggg tgttcaactc
     9841 tccgatcagc gaagcgtgca tctgcggtac cgggtgcggc gcggccatga acggattgcg
     9901 gcccatcgtc gaactgatgt acttcgattt cgcgctgatg tcgtccgacc aaatcagcaa
     9961 tcaggcagcg aagtggcatt atatgagcgg cgcacagacc gaggtgccgc ttgtcatccg
    10021 cgcctccgcg ggcgccggca agggttacgg cggccagcat tcgcagacgc ttgaatccat
    10081 gttctgccac atccccgggc tttacgtggt ttatccggcg acgccggccg acgcgaaggg
    10141 tatgcttaag tcggcgatcc gcgacaacaa tccgatcctc ttcgtcgagt cgcaggcgct
    10201 ctacggcatg aaaggcccgg tgcccgaagg cgagtatctg gttccgctcg gcgttgccga
    10261 ggtcaaacgc gaaggcagcg acatcaccct ggtcgcctgg ggcccgctgg tgcatgactg
    10321 cttcacggcc gccgagaaat tgaaggccga gaagaacgtc agcgccgagg tcatcgacct
    10381 gcgcagtctg gtgccgctgg acatcgaaac cgtgttgcgt tcggtgagga agaccggccg
    10441 ttgcgtcgtc gccagccagg cgattcacat cggcagctac accggcgaga tcgcctcgac
    10501 gattcaggac ttggcattcg attatctcga tgcaccggtc aaacgcgtcg gcgccaagaa
    10561 cggcatcgcg ccgcaatcgc atattctcga agcggcgttt ttgccgaatg tcgacgacat
    10621 ccttgccgcc gccaacgcca tcttgtaggg cacgccatgg ccaccccgat tatcatgccc
    10681 aagttcggtc agatgaccga agaaagcgcc atcatcgaat ggctcaagaa ggaaggcgat
    10741 gcggtcgcca agggtgacat tctattcacc gtcgagaccg acaaatcggt catggaggtc
    10801 gagagcttcg aggaaggcac gttgctcaag atcgccgtga agccgggcat cagcgttccc
    10861 gtgcagagtg tcgtcggatt tctgggcaaa cccggcgaac cggttccgga agcggcgccg
    10921 ccggtggcgc ctgcgcccgt caagacggaa ccgcctaagg cggcggtacg tgtcgctccc
    10981 gtcgcggcgt cttcgcccgc gattccggtt gctgcgccgg cagcggtagc gtcaccggtt
    11041 gtcgtcgcag ctccggttga gccggccctg ttccgcatca gcccgcgcgc cgcagccctg
    11101 gctcgcgact gcgttatcga tccggccggc atcaagggat ccggtccggg cgggcgcgtc
    11161 gtcgagaaag acgtaaaggc ctatctcgaa gcgaagggat attaccggct tcgcgtttcg
    11221 cccctcgcca agcagcttgc ggccaaagag agcctcgatc ttctgactat tcatagcgcg
    11281 gacgcatcgg gccggatcgg cgtcgtcgat gtcgaacgcg ccgtggccga aaagccgaag
    11341 ccgatgagcc agatgcgcca gatcatcgcg cagcgtctga cgcaaagcgt ggtcaccgcg
    11401 ccgcattttt acgtcacggc cgaagtggac atgaccgact tggttggatt ccgcgcccaa
    11461 ctcaaggcaa agggcgcgcc ctacacggtc accgacttca tttcgctggc ggcggttctt
    11521 gccctcaagg acttcccgaa cgtgaacagt tcgacggacg gcaggaccgt ccgctggaac
    11581 agtcacgtcc atctcggcat tgcggtgtcg ctggagcagg gtttggtcgt gccggtcatc
    11641 cgcaatgcgg acgagctgac gatggccgaa ctgaacaccc gcgcgaagga acttaccgag
    11701 aaggcgcgcg ccggcaaact cacgcccgac gaaatggccg gcggcacctt cacgatctcc
    11761 aacatgggca tgctcgacgt ggagaacttc acggcgatca tcaatcccgg cgaatcggcc
    11821 atccttgcgg tttccagcgt gttggagaaa cccgtggtgc gcgacggcaa ggtggtgatc
    11881 cggtcgataa tgaagatgac tctgtcgtca gatcaccgcc tgattgacgg cgcgatggcg
    11941 gctcggtttg ttaatgctct gaagaagaag attgaggacg tcgaattgtg gaggtccttg
    12001 gtgccgtaat catcggtgcc ggtcacagag aataaaacag cgcaacagca caatcggatg
    12061 aggaaacaat ggccaagatt caagagcctt tggcgcagga aacgatcgaa agccacaaca
    12121 aacggcacct gggcgcgatc cgggaagact atgacgcgct cggccgcacg ctggcacgcc
    12181 gcaagatcga tatcgatgcc gtcaagcggc gcgtcgccgg tttttccgtc gccgtgccga
    12241 gttggggcgc gggcgtaggc gggactcgct ttgcccgctt tcccattccc ggcgagccga
    12301 cgaacgttca cgagaagctc gtcgattgcg cagtgatcca acaattgtgc cgcatgacgc
    12361 cgcgcgtttc cacccacttc ccatgggatt atgtcgacga tcccgacggc cttcgtcagg
    12421 aagctgagca actgggccta agcttcgacg ccgtgaattc aaacacgttc gaagatcagc
    12481 caggacaacc tctgagctat cggtacggca gtctcagcgc aactgacaag gccgttcgcg
    12541 aacaagcggt caagcacaat atcgactgca tccggatcgg ccggaagctt ggttccaagg
    12601 ccctgacggt gtgggtcggg gacggcacca attttcccgg ccagcagagt ctgcgcgact
    12661 cgcttgaccg ctatctcgaa ggcgcgcacg ccatctatgc ggagctgccc gcggactggc
    12721 ggatgttcat cgagcacaag ctctatgaac cggccttcta ttcgacggtc atctccgact
    12781 ggggtaccaa catcatggtg gcgaaggaac tgggcccgaa ggcgaagtgc cttgtcgatc
    12841 tgggtcacca cgccccgaat gtcaacatcg aacagatcgt ggcgcgactc agccgcttcg
    12901 gcaagctggg cggtttccat ttcaacgaca gcaaatacgg cgacgacgac ttggatacgg
    12961 gctcgatcaa cccccatcag ttgttccttg tgttcaacga actggtggag gccgaacgcg
    13021 ccgctccgaa ggatttcgat ccggcgtaca tgatcgatca gtcgcacaac atcaccgatc
    13081 cgatcgaaag cctgctggcg agcgcagaag cgatcgcagg cgctttcgcg cgcgctcaga
    13141 tcgtggaccg cgacacgctg gcgacctgtc gtgaacaaaa cgacgtcatg gaggctttcc
    13201 ggacattgcg gcatgcctac gaggtggacg tggcccccat cctggcgctg gtgcgcgtcg
    13261 aggcgggcgg ggcggccgac cccatcctgg cgtatcgcga atcggcttgg cgcgcccgca
    13321 aggcccagga acgcagtcct tcgaaattgg gctccggtat cgtatgatta gcgcgtgaat
    13381 agcgcgggcg tggcattttt cttgacaaat gccggaccat gtcctgtgac ttgtgcacat
    13441 gagagtgaaa atgcacgcaa ccgagcggga tcgagtcatt ctgagccttc ttgaagaaga
    13501 aggttttgtt tccttccgcg agttgtcaca cagggtttca gcctcgccgg cgactttgcg
    13561 gcgggatctc gagcgcttgc agacggtcgg caagctggtg cgcgtccggg gcggcgtaca
    13621 gccgaccggc ggagtcgatc ccgtgagcgg gctgcatctg caaggcgtcc cgttccatga
    13681 gaatatcaac cggaaccgtg ccgcgaagga ggccatcggc aaagccgccg ccgaattatg
    13741 ccggccgggc gaagccatca tcatcgatgg cggcagcacg acgttgcaga tgtgccctca
    13801 cttggagtcg ctgaggctgc aggtactgac gaactctctg catatcgtca gcgcgctgct
    13861 gcaacatcag aacaccggcg tttccatccc cgcctgcgtg gtgttccgct agcagaatat
    13921 cgtgctcgat cctttcgagg atgtgaaaac ccgcaccttt cacgcttcgc ggatgttcgt
    13981 cggcgcggcg gcggtttccc gtcacggcct cttgcaaagc gacatcatcc tcgtacaggc
    14041 ggagcgcaaa ctgctcagcc aggcggacca gttgatcgta ttggtggaca gcagcaagtt
    14101 cgccgcctcc gccggacata tgttatgtga actgtccagg gtgcatacct tgatcactga
    14161 cagcaagatc gacgacgctt ccgccaagat gatcaaaagc catggcgtcg agttgattac
    14221 ggtggacgtc taagattcaa tccaattgag ccggagcggc gggcgaaacg gcttgcgcca
    14281 gcgcccggcg ctcgaacgcg aagggtccgg gcgccggcgc gaccgcctta gttctcgttg
    14341 cggcgatgtt ttctttgctt gacaggcttt gacatcagca ccggctgcaa gggttttccg
    14401 gggccggacg cggcaagaag cgcatcggta attaagccgg acacgtcttc cgccaccacc
    14461 ggcgggcggg catccgccgc cacgctggta agctcgacgt cgcgcagaat gatgccgcgc
    14521 acgtgacgca tgtagaggcc ccaggccggc atgacgccga acatatcggg gtcgggatat
    14581 ttttccggca cttcggggag cttgatcgcg gcgtcttccg ccgtcccgcc ccccggatag
    14641 atcagccgga tgccgctcaa ggtgacgtcc tcgaccatgg ctccgggtac ggcggtgatg
    14701 ctggcgcagt agcgcttgtc caagcagtag gcatcgatgt cgctgatcgt gacgcggcgg
    14761 agcgccgcaa tcggcgttcc cgcgggcgca cgccggcgat cgccgagccg caggaagatg
    14821 ggcgcggccg tcgtgtcgcg catcacgata ttgttcacga ccacgtcttc caatacgccg
    14881 ccgtcgacgc tttctatcgc cagtccgttg ccgcaatcga agacgcaatt ggaaatggcg
    14941 atattgcgga agccgccgtt ggattcggtg cccagcttga tgcgcccgct ggtcaggtgc
    15001 ggttcgatat tcttcagctg gctcctcttg taggtgccgt ccaacaagga gccgacatcg
    15061 tagccgctga cgtgacaatt ggtgatcgtc acgttctatg tgggtcttgc tgtgcccagt
    15121 gccagcgagc ttttcaacac gatggcgtcg tcggtcggcg agttgaccga caggttcgac
    15181 aggcgcgcgt tgcgtacgac gtcgagatcg atgccgtcgc ggttggtgtc gatcctcata
    15241 ttgtcgatag tgagattgtc cacgccggtg gcgagaatgg cgaagtggcc gccgcgcaga
    15301 atcgaaacgt cgcggatggt cacgttgcgt gaattcttca aggcgatcgc tttgttgccc
    15361 tttccgctca tcgccttgac ctcggcatta tggctcgcaa gctgcgcatc cagttcgggg
    15421 ctggcaggcc agtctctgcc aacgccgatg ctccagggcg cattgggact ttcgcgggtc
    15481 aggccatctc cgtcgatcaa gcccggtccc atgatggcaa tgtcttccag atcttcgccc
    15541 cagatcagac tgttgcggaa atagctgtgg ccgtaatctt gataagcgtc ccaaggattg
    15601 gcttccggca aatcgtagct gccgccatgc ttggccggat cggcagctat cagaacggcg
    15661 cctgaagcga agagcagggt gatgtgactc ttcagccgga tcgtgaagct cagatagcgg
    15721 cccgtcggca gataaacagt tccgccgcca gcggcagatg cagcatcgat ggcgcgttga
    15781 atcgccgcgg tatcgagagc cgtgtcatta cctttggcgc cgtagtcgcg aacatcgaag
    15841 acatcgatgg gtctcgcaaa tgcgcttgac gcaaaagcgg gaaggaaagc caaacttgtg
    15901 ctggctagaa actgacggcg aggaacctgc agcatcatcg gaatactcca aggtggatca
    15961 aacaggcgtg aaacctagca cggataagat tgtaattgag cgaatttgtg atagttatgg
    16021 tctataatgc acagtacaac acgcactttg tagtgaccag gcaaccgcac ggctaggaga
    16081 atcacccgga gccgtgcttc tgcagtttaa cggcagcggc gatcaacgag gtgttgcgcg
    16141 atgaagaagc tgcctgggat tttttcccga caaaagaggc tcatcgctcc ccggttgtgg
    16201 aaggtcctgc ctttactgct tttttcttct ctagcgatgg ccggtccctc cctcgctgcg
    16261 gaacccacgc gacaccatgt cgccaaagcg tcccgtgata tcctggtgaa cggattccgc
    16321 acgccgcccc aagcggccaa gccgcgcgtg tggtggcatt ggatgaacgg caacatcacc
    16381 aaggacggca tccgcaaaga tatcgaatgg atgaagcgca tcggtattgg tggcatggac
    16441 gccatcgacg ccaccatcga gacgccgcag gtggtgaagc accgcctcat ctatatgact
    16501 ccggagtgga aggatgcctt ccgctattcc acggaacttg ccgacaaata cggaatggag
    16561 ctggcgatca attcttcgcc cggctggagc atgaccggcg gtccctgggt aactccgcag
    16621 caagcgatga agaagctggt ttggagcgag accgaggtcc aaggcggtaa gccgttccac
    16681 ggcgtattgc ctaagccgcc agacaatccc ggtccgttcc agaacatcaa gaacgccccg
    16741 ggctacgatc agttctcgcc cgcggcggcg tcattctatc gcgacagtat cgtggtcgct
    16801 taccgcgcgc cggtcggcaa acctgtggtc tccgaagcgg tatcggattc gggcgcaatc
    16861 gatgccgccg tcctctccga cggcgatctg acgaacggcg tcacgctgat acccaaagaa
    16921 gccgacaaag acgtctggat tcgtctcgcc tataaggatc cggtacggat ccagggtctg
    16981 acgctggcga tatcgacgcc cgacggcctc ggctttgcag cggccgtaga ggtcagtgac
    17041 gacggccaga gctggcgcca tgtcgccgat gtcccgcaaa ggcagcagat ccgcaggttt
    17101 gcgctgctgc aacagaccat ttccttcccg cccgtcaccg gccgtttttt ccgcgtcgtg
    17161 ctgcgtcctc atgcgccgat cccgacaagt ctgcgcatcg tcttcaatgc gcccggggtg
    17221 gtggtcaata ctcgcccgac cccagacaaa ccttcctatc gtgtttatga gctggcattt
    17281 catgccggcg cgac
//
LOCUS       DASWPU010000588         1993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_67003,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000588 DASWPU010000000
VERSION     DASWPU010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67003"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /gene="argJ"
                     /locus_tag="VGC27_12465"
     CDS             <1..911
                     /gene="argJ"
                     /locus_tag="VGC27_12465"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY0283423.1"
                     /translation="GCRASEVFLASTGVIGESLPAEKITKLLGALAQDMSSAGWRAAA
                     EAILTTDTYPKMATVTASIDGKKVTINGICKGSGMIAPDMATMLAFVFTDANLPASVL
                     QELLDAGVKPSFNSITVDSDTSTSDTLLLFATGKGARHPAITKASDKRLANFRAKLDG
                     VLLDLALQVVRDGEGAQKLIRVDVTGAENDVAAKRIAFSIANSPLVKTAIAGSDANWG
                     RVVMAVGKAGEKADRDTLTIRFGNQLVAEKGDRAAKYDEAAATKAVSGPEVEIVVDLA
                     LGKGTARVWTCDLTHGYIDINGSYRS"
     gene            960..1367
                     /locus_tag="VGC27_12470"
     CDS             960..1367
                     /locus_tag="VGC27_12470"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEY0283424.1"
                     /translation="MTQVKLVLVAACALIDPDGRVLLAQRPAGKNLAGLWEFPGGKME
                     PGERPEDTLIRELREELGIEVKEPCLAPFTFASHSYADFHLLMPLYVCRRWEGTPQAR
                     EHPALKWVRPKDMAGYPMPPADIPLVPMLRDLL"
     gene            1376..1507
                     /locus_tag="VGC27_12475"
     CDS             1376..1507
                     /locus_tag="VGC27_12475"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0283425.1"
                     /translation="MRDERGATAIEYALIASIVSIVIVSALAIIGSDLKAKFESIAF"
     gene            1551..>1993
                     /locus_tag="VGC27_12480"
     CDS             1551..>1993
                     /locus_tag="VGC27_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose 1,5-bisphosphate carboxylase"
                     /protein_id="HEY0283426.1"
                     /translation="MSSRIEVLYRVRSDAKSIAARAEGIAVEQSVEMPVEPIGDPFVL
                     GEVLGRVEDINDIGNGAFDVRIALASVTTGFEAGQLFNMLFGNSSIQDDITLVDARFP
                     EELVGAFGGPNHGLAGLRVRVNANGALTCSALKPQGSTSAVLAELA"
BASE COUNT          361 a          641 c          630 g          361 t
ORIGIN      
        1 tcggttgccg ggcctcggaa gtgtttctcg cctccaccgg tgtcatcggc gaatcgctgc
       61 cggcggagaa gattacgaag cttttgggcg cgctggcgca ggatatgtcg tcggcaggct
      121 ggcgcgccgc cgccgaggcg attctgacca ccgacaccta ccccaagatg gcgaccgtca
      181 cggcgtccat cgacggcaag aaggtcacca tcaacggcat ctgcaaaggt tcgggcatga
      241 tcgcgcccga catggccacg atgctggcct tcgtcttcac cgacgccaat ctgccggcct
      301 ccgtgctgca ggaattgctc gacgccggcg tcaagccttc gttcaactcc atcaccgtag
      361 atagcgacac ctccaccagc gacacgctgc tgctgtttgc gacggggaag ggcgcccgcc
      421 atcccgccat caccaaggcg agcgataaac gtctcgccaa tttccgggcc aagctcgacg
      481 gcgtgttgct cgatctggcg ttgcaggtcg tgcgcgacgg cgagggggcg caaaagctca
      541 tccgcgtgga cgtcaccggc gccgagaacg atgtcgcagc caagcgcatt gcgttttcga
      601 tagccaattc gccattggtg aagaccgcca tcgccgggtc cgacgccaat tggggcaggg
      661 tggtgatggc cgtgggcaag gcgggcgaga aggccgaccg cgacacgctt acgatccgct
      721 tcggcaacca gctggtggcg gagaaaggcg accgcgcagc caagtacgac gaagccgccg
      781 cgacgaaagc cgtttccggt cctgaagtcg aaatcgttgt cgatctggcg ctcggcaagg
      841 gcacggcgcg cgtctggacc tgcgacctta cccacggcta catcgacatc aacgggtcgt
      901 accggagtta agtcttaatc cgcccttaag cgccctcgga ccagaatgga gccgatcgca
      961 tgacgcaggt gaagctggtt ctggtggcgg cctgcgcctt gatcgatccc gacgggcgcg
     1021 tgcttctggc gcaaagaccc gccggcaaga atttggctgg gctatgggaa tttccgggcg
     1081 gcaagatgga gccgggcgag cgcccggagg acacgctgat ccgcgagctt cgcgaggaac
     1141 tggggatcga ggtgaaggag ccatgtttgg cgccgttcac ctttgcatcg cacagctacg
     1201 cggactttca tttgctcatg ccgctttacg tctgccgccg ctgggagggc acgccccagg
     1261 cccgcgagca tccggcgctc aaatgggtgc gccccaagga catggcgggc tacccgatgc
     1321 caccggcgga cattccgctc gtcccgatgc tgcgcgatct cttgtaagca acttcgtgcg
     1381 cgacgagcgc ggtgctacgg ccatcgaata tgctttgatc gcctcgatcg tctccatcgt
     1441 catcgtcagc gctctcgcga tcatcggcag cgacctcaag gccaagttcg agagtatcgc
     1501 gttctaattt ttcttgcgca caggccctcg gccgctgcta tggtcgggcc atgagcagca
     1561 gaattgaagt cctctaccgc gtccgcagcg acgccaagtc catcgccgcc cgcgccgaag
     1621 gcattgccgt cgagcagagc gtcgagatgc ccgtcgaacc gatcggcgat cccttcgtcc
     1681 tgggcgaggt cttgggccgg gtggaagaca ttaacgacat tggcaacggc gcgttcgacg
     1741 tgcgcatcgc gctcgcttcc gtcaccacgg gcttcgaggc cggccagctc ttcaacatgc
     1801 tgttcggcaa cagttcgatt caggacgaca tcacgctggt ggacgcgcgc ttccccgagg
     1861 aactcgtcgg tgccttcggc ggaccgaacc acggccttgc cggcttgcgg gtgcgggtaa
     1921 acgcaaatgg cgcgctcacc tgttcggcgc tgaagccgca aggctcgaca tccgccgtct
     1981 tggccgaact tgc
//
LOCUS       DASWPU010000589         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_29258,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000589 DASWPU010000000
VERSION     DASWPU010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29258"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VGC27_12485"
     CDS             complement(<1..320)
                     /locus_tag="VGC27_12485"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283427.1"
                     /translation="MLKPRSDLQPNSFDFENLPLITANGFREYDARWLFGKEINLLGV
                     QALGQGLGTFLHEVGATKVVTGHDYRSYSLSIKQALTLGLMNAGCEVLDIGLATTPAA
                     YYAQ"
     gene            468..1343
                     /gene="cobA"
                     /locus_tag="VGC27_12490"
     CDS             468..1343
                     /gene="cobA"
                     /locus_tag="VGC27_12490"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY0283428.1"
                     /translation="MSNDLINAAERTAVQRLAKALALPAFDKGWVWLAGAGPGDPGLI
                     TALGLHAIAAADIILYDALVNEALLELARPGAEIVYAGKRAGKASCKQSDISRTLVSL
                     ARKGKRVLRLKGGDPFVFGRGAEEALTLARAGVPFRIVPGVTAGIGGLAYAGIPLTHR
                     DTNHAVTFLTGHGADGKLPRLDWAAIARGSPTIVLYMARKYAGEIAAKLIAAGRDAGE
                     PAAVVSNASFADQSVRVTTLANLGQAAAESAAPAILIIGENVKLRAGLDWLGALAGKT
                     LDPESPEKTLLSGAG"
     gene            complement(1351..1818)
                     /locus_tag="VGC27_12495"
     CDS             complement(1351..1818)
                     /locus_tag="VGC27_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY0283429.1"
                     /translation="MPVASWILLLVVFAVAGRFLIEADPVRFPGFPAAASNAINTGFT
                     LCAERYQANCVVDGDTIHVNGTRIRVEDIDAPETHNPRCASELVLGEQAAQRLLALMN
                     AGPFKIVYTGGRDTDEYGRELRRIERNGRSLGGILVSEGLARLWDGARHPWCN"
     gene            2100..2630
                     /locus_tag="VGC27_12500"
     CDS             2100..2630
                     /locus_tag="VGC27_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283430.1"
                     /translation="MAKAQFHKNQRVFVKPVGTWALIEHVVPQWAKGIEEPIRVFYDC
                     GLGREFAAEELQAEEPLADRGEITTENWRVTRARNKWQPTEDCARHPIPGTYPVVITG
                     QADWGGWRVPGAEYDLDPFRIERQARLIAAAPRFVAFAKALVEWARKSGEEMPAELAG
                     LAHAAQDLLTGIERQG"
BASE COUNT          541 a          835 c          874 g          446 t
ORIGIN      
        1 aactgcgcgt aataggctgc cggcgtcgtc gccaggccga tgtccagcac ttcgcagccc
       61 gcattcatca gcccgagcgt gagcgcctgt ttgatcgaca gcgaatagga acggtaatcg
      121 tggccggtga cgaccttggt cgcgcccacc tcgtgcagaa aagtgcccag cccctgcccc
      181 agcgcctgca cgccgagcag gttgatctcc ttcccgaaca gccagcgcgc gtcatattcg
      241 cgaaagccgt tggcggtaat aaggggaaga ttttcgaaat cgaagctatt gggctgcaaa
      301 tcgctgcgag gtttgagcat ggctttcccg gttttggacg gcccgctgtc tagcccaacg
      361 gaacagaaaa atcagtcgaa tatggggctt tcaccaccaa gaaggtcgca ggctattttc
      421 cgcaaacggc atgtggtaca cttagtttca caaaagagaa gtgtgtaatg tcgaatgacc
      481 ttataaatgc cgccgaacga actgccgtcc agcggctggc gaaggcgctt gccttgcctg
      541 ccttcgacaa gggctgggtc tggctggcgg gagcgggtcc gggcgatccg ggactgatta
      601 cggcgctggg attgcacgcc atcgcggcag cggacatcat tctctacgac gcgctggtca
      661 acgaggcgtt gctggagttg gcgcggccgg gcgccgagat cgtctacgcc ggtaagcgtg
      721 ccggaaaggc gtcgtgcaag caaagcgaca tctcgcgcac gctggtgtcg ctggcgcgca
      781 agggaaagcg cgtgctcagg ctgaaaggcg gcgatccttt cgtcttcggg cgcggcgcag
      841 aagaagccct tacgctggcg cgcgccggcg tgccgttccg catcgtgccg ggggtcacgg
      901 cgggcatcgg cgggctggcc tatgccggca ttcccctgac tcatcgcgat accaaccacg
      961 ccgtcacttt tctcaccggc cacggcgcgg acggcaagct gcccaggctc gactgggccg
     1021 cgatcgcccg cggctcgccg acgatcgtgc tttacatggc gcggaaatat gccggcgaga
     1081 tcgccgccaa gctgatcgcg gcgggccgcg acgccggcga gccggcggcc gtcgtcagca
     1141 acgcgtcttt cgccgatcag agcgtgcgcg tgacgacgct ggcgaacctc ggccaagccg
     1201 ccgccgaaag tgccgcgccc gcgatcctga tcatcggcga gaacgtgaag ctgcgtgccg
     1261 ggctggattg gctgggcgct ctggcgggca aaacgctcga tccggagtcg ccggaaaaaa
     1321 cgcttctgag cggcgcggga tgaccccgat ctaattgcac cagggatggc gtgcgccgtc
     1381 ccacaaccgg gcgagacctt cgctcaccag aatgccgccc agggaacgac cgttgcgctc
     1441 gatacggcga agctcgcggc cgtattcgtc ggtgtcccgg ccgccggtat aaacgatctt
     1501 gaatggcccg gcattcatca acgccagaag acgctgggcg gcttgctctc cgagcaccag
     1561 ctccgacgcg caacggggat tgtgcgtttc gggcgcgtcg atatcctcaa cgcggatacg
     1621 tgtgccattg acatggatcg tatcgccgtc caccacgcag ttcgcctgat accgttccgc
     1681 gcagagggtg aagccggtat tgatggcgtt actggccgca gcggggaaac ccggaaaccg
     1741 caccggatcg gcctcgatca gaaatcgtcc ggccacggcg aagacgacaa gcaatagaat
     1801 ccatgaagca acaggcaccc tatagcgcca atgccgggct ttgggcggcg tccgcgggcg
     1861 gcgtcggtat tcggtgaatt cgtccaaatg cgactgacct cgcgcagcaa tcgcgcaagc
     1921 gtaggctggc tgcattaacg gcgcgtttgc ggcgaggctg gcggttaagg atgaatcgtg
     1981 aggcggtcag gaggcggatc gtctccttgc agaaagccac aggaccgcgg ccgttcggaa
     2041 cttaacgacc acttaaggca gacgtcctag cttcacctca gcggaaacgg ataaatgaaa
     2101 tggccaaagc gcaattccac aagaaccagc gcgtattcgt gaaacccgta ggaacctggg
     2161 cgctgatcga gcacgtcgta ccgcaatggg ccaagggcat cgaggagccg atccgcgtgt
     2221 tctacgattg cggattgggc cgcgaattcg ccgccgagga actgcaagcc gaagaaccgc
     2281 tggccgaccg cggcgagata accaccgaga actggcgggt cacgcgcgcg cgcaacaaat
     2341 ggcagccgac ggaagattgc gcgcgccatc cgataccggg cacctatccc gtcgtcatca
     2401 ccggacaggc cgattggggc ggctggcgcg tgccgggcgc ggaatacgac ctcgatccct
     2461 tcaggatcga gcgccaggcg cggctgatcg ctgccgcgcc cagattcgtg gccttcgcca
     2521 aggctctggt ggaatgggca cgcaaatcgg gcgaggaaat gccggcggag ctggcgggtc
     2581 ttgcgcacgc cgcgcaggac ctgctaaccg gtatcgaacg ccaaggctga gtccttcccg
     2641 acaatccgcc aaatcgacgc cgatgccgca aatcagcgcg cgccacgaaa ccaatg
//
LOCUS       DASWPU010000590         4038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_109582,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000590 DASWPU010000000
VERSION     DASWPU010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109582"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VGC27_12505"
     CDS             <1..687
                     /locus_tag="VGC27_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0283431.1"
                     /translation="LPAAPAAAESMIVERDIRLVELTGARYHFGQISCRASLEAIVAA
                     KRRGLPITCGVAAHHLALNELDVGPYYTFMKVKPPLRSEADRAAMVEGVASGAIDAIV
                     SGHDPQAADTKRQPFAQAAFGAVGLETLLPVALSLAHNGHTDLVHVLKALTATPASIL
                     GLPGGTLAKGMPADLVLIDPDVPFVVDPLKLHSRARNTPFNGRKFHGRATKTYVGGEC
                     VFDHSAETRK"
     gene            684..1304
                     /gene="plsY"
                     /locus_tag="VGC27_12510"
     CDS             684..1304
                     /gene="plsY"
                     /locus_tag="VGC27_12510"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY0283432.1"
                     /translation="MITGASILWPNVLLCLAFGYLLGSIPFGLIITRLAGLGDVRKIG
                     SGNIGATNVLRTGKKWAAALTLLLDGGKGAAAVVIGYAAFGINGGAIAGIAVVLGHIL
                     PVWLRFKGGKGVATFMGVMLAAYWPTGLLAIVTWLVVAYIWKMSSLAALAAAALAPAY
                     TIALGNLPYALLAMVLAIIIFVSHHENVHRLWRGEEPRIGSRKRSD"
     gene            1297..1500
                     /locus_tag="VGC27_12515"
     CDS             1297..1500
                     /locus_tag="VGC27_12515"
                     /inference="COORDINATES: protein motif:HMM:NF045295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283433.1"
                     /translation="MIDRRLSEVERRAWLRLARAPNVGPVTFAQLLARFGSASAALAE
                     VPRLVRRGGGDTAPLPSEADARR"
     gene            1510..2409
                     /gene="dprA"
                     /locus_tag="VGC27_12520"
     CDS             1510..2409
                     /gene="dprA"
                     /locus_tag="VGC27_12520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY0283434.1"
                     /translation="MAKLGGRLIASADPDYPRGLAALDAPPPLISVIGSEAVLRREMI
                     AIVGARNASALGRKLAHRLAAELGAAGLVVVSGMARGIDTAAHEGALKTGTCAVVAGG
                     IDIVYPPENLPLYERIKAEGVIVSEMPVGQPPQARHFPRRNRIISALARGVIVVEAAE
                     GSGSLITANFALEQNREVFAVPGSPLDPRAKGANRLLRQGAVLTETADDVLGVLKPIL
                     GGVFQEPSYRLGDAPPEGEPSEEETDRVRSVVEEALGPAPVAVDELIRLCGAPAAAVL
                     TVLLELELAGRLARHPGNRVSWL"
     gene            complement(2379..2591)
                     /locus_tag="VGC27_12525"
     CDS             complement(2379..2591)
                     /locus_tag="VGC27_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283435.1"
                     /translation="MITTPLQSGSQKLPEMGPAETAQYTKETLESLRKIALKQKQVLL
                     AHLLELAAVEAKALAQSQSQETRFPG"
     gene            2901..>4038
                     /gene="topA"
                     /locus_tag="VGC27_12530"
     CDS             2901..>4038
                     /gene="topA"
                     /locus_tag="VGC27_12530"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY0283436.1"
                     /translation="MNVLVVESPAKAKTINKYLGPGYIVLASFGHIRDLPSKDGSVKP
                     DDDFVMTWHVDERAQKRIKDIADALKGADKLILATDPDREGEAISWHLLEVLRQRKAL
                     GKARIERVVFNAVTKAAVLEAIANPREINDELVDAYLARRALDYLVGFTLSPVLWRKL
                     PGARSAGRVQSVALRLVCERESEIELFKAQEYWTVDAEVRAPAGSFQAHLTYLDGKKV
                     EKLSLANEAAAMRAVTAIEKGRFSIGNVESKPVKRHPSPPFITSTLQQEASRKLGFAA
                     KRTMQVAQQLYEGVDVGGEQVGLITYMRTDGVTMDGAAIREAREMIGQRYGKKYVPHS
                     PRAYSSKVKNAQEAHEAIRPTSFARAPELVSRRLDSDQARLYELI"
BASE COUNT          698 a         1321 c         1274 g          745 t
ORIGIN      
        1 cttcccgccg cccccgccgc cgccgagagc atgatcgtgg aacgcgatat ccgcctcgtc
       61 gaactgaccg gcgcgcgcta tcacttcgga cagatttcct gccgcgcctc gctggaggcc
      121 atcgtggcgg cgaagcggcg cggcctgccc atcacttgcg gcgtcgccgc gcatcatctg
      181 gcgctgaacg agctggacgt ggggccctac tacaccttca tgaaggtgaa gccgccgctg
      241 cgctccgaag ccgaccgggc cgcgatggtg gaaggcgtcg cctccggcgc gatcgacgcg
      301 atcgtctccg gccacgaccc gcaggcggcc gacaccaaac gccagccttt cgcgcaagcc
      361 gcgttcggcg ccgtgggatt ggaaacattg cttccggtgg cgctcagtct cgcccacaac
      421 ggacataccg accttgtcca tgtgctgaaa gccctgacgg caacgccggc gtccatcctc
      481 gggcttcccg ggggaactct ggccaagggc atgccggcgg acctggtgct gatcgatccc
      541 gacgtgccct tcgtcgtcga tcccttaaag ctccactccc gtgcccgcaa cacgcctttc
      601 aacggccgca aattccatgg ccgggcgacg aaaacctacg tcggcggcga atgcgtgttc
      661 gaccactccg cggagacgcg gaaatgatta caggcgcctc catcttgtgg ccgaatgtgc
      721 tgctttgcct ggcgttcggc tacctgctgg gttccattcc cttcggcctg atcataacgc
      781 ggctcgccgg cctgggcgac gtgcgcaaga tcggctcggg caacatcggc gccaccaacg
      841 tgttgcgcac cggcaagaaa tgggcggcag cgctgacctt gttgctcgac ggcggcaagg
      901 gcgccgccgc tgttgtgatt ggatacgccg ctttcggaat caatggcggg gcgattgcgg
      961 gaatcgccgt ggtcctcggc cacatccttc cagtctggtt gcgcttcaag ggcggcaaag
     1021 gcgtggcaac cttcatgggc gtgatgctcg ccgcttattg gccgaccgga ttgctggcga
     1081 tcgtaacctg gctggtcgtc gcctatatct ggaaaatgtc ctcgctggcg gcgctggcgg
     1141 cggcggcgct ggcgccggcc tacacgattg cgctcggcaa cttgccgtat gctttattgg
     1201 caatggtcct ggcgatcatt atcttcgtct cccaccacga gaacgtgcat cggctctggc
     1261 gcggcgagga gccgcgcatc ggcagcagga aacgcagtga ttgaccgtcg cctttcggag
     1321 gtcgaacgcc gggcttggct caggctggcg cgcgcgccca atgtcggccc cgtcactttc
     1381 gcgcaattgc tcgcgcgctt cggttcggca tccgccgcgc ttgcggaagt tccgcgcctg
     1441 gtgcgccgcg gcggcggcga tacggccccg ctgccgagcg aggccgacgc gcgccgctaa
     1501 atcgacgccc tggcgaaact cggcggccgt ctgatcgcct cggcggatcc ggactatccg
     1561 cgcggattgg cggcccttga cgcgccgccg cctctcatct cggtgatcgg cagcgaagcg
     1621 gtgctgaggc gcgaaatgat cgccatcgtc ggcgcgcgca atgcttcggc actgggccgc
     1681 aagctggcgc accgccttgc ggccgagttg ggcgcagccg gcctcgtcgt ggtttccgga
     1741 atggcgcgcg gcatcgatac cgccgcgcat gaaggcgcgc tcaagaccgg tacctgcgcg
     1801 gtcgtcgccg ggggcatcga catcgtctat ccgccggaaa acctgccgct ctacgaacgc
     1861 atcaaggccg aaggggttat cgtttcggag atgcccgtgg ggcagccgcc gcaggcccgc
     1921 cattttccgc gccgcaaccg gatcatctcg gcgctggcgc gcggagtgat cgtggtcgag
     1981 gcggcggaag gctccggctc cctcatcacc gcgaacttcg cgctcgaaca gaaccgcgag
     2041 gtctttgccg tgccgggttc gccgctcgac ccgcgtgcca aaggcgcgaa ccggctgctg
     2101 cgccaaggcg ccgtgctgac cgaaaccgcg gacgacgtgc ttggcgtgct gaaaccgatc
     2161 ctgggcggcg tattccaaga accctcgtac cgcctcggcg acgctccgcc ggagggcgaa
     2221 ccttcggaag aggagaccga ccgggttcgt tccgtggtag aagaagcgct cggtcccgcg
     2281 ccggtggccg tcgacgaact gattcgttta tgcggtgcgc ctgccgcggc cgttctgacg
     2341 gttctcctgg aattggagct ggccggcagg ctggcccgtc accccggaaa ccgggtttct
     2401 tggctctgag actgcgccag agccttcgcc tcgacggccg ccagctccaa cagatgggca
     2461 agcagcacct gcttttgttt gagcgctatt ttcctaagag attcaagggt ttccttggtg
     2521 tactgcgccg tttccgccgg acccatttcc ggcagcttct ggctgccgct ttgaagcggt
     2581 gtggtaatca tttgggcaat cccatctacg cctacctaac aggaaatcta tggttatgac
     2641 gcgccgtatg caaccacaag gatgttagtt gatattttca cttactgtag attgcgcttc
     2701 atgtgcataa aggtcgctaa attggcgcaa ataggaacac tttcttaatc gcggaattga
     2761 tctcgctcga aatcgcgcgt tgacaccgct ccgccgctta catcagtgtc cgcccgccta
     2821 atgagccgct gacatttggc ggtttccgcg gttcccggcc cctccggaaa catgcggaca
     2881 ccgcgtgagc cccatatcgc atgaacgttc tcgtcgtcga atccccagcc aaagccaaga
     2941 caatcaataa gtatctgggc cccggctaca tagtacttgc cagtttcggc catatccgcg
     3001 acctcccctc caaggacggt tcggtgaagc cggacgacga ctttgtcatg acctggcacg
     3061 tggatgagcg tgcccaaaag cggatcaagg acatcgccga cgcgctcaag ggcgccgaca
     3121 agctgatcct ggccaccgac ccggatcgcg agggcgaggc gatttcctgg catctgcttg
     3181 aggtcctcag gcagaggaag gcgctgggca aagcccgtat cgagcgcgtg gtgttcaacg
     3241 ccgtgaccaa ggcggccgtg ctcgaagcca tcgccaaccc gcgcgaaatc aacgatgaac
     3301 tggtcgatgc ctatctggcg cgccgcgcgc tcgattatct ggtcggcttc acgctgtcgc
     3361 cggtcttgtg gcgcaagctg cccggggcgc gctcggcggg gcgcgtgcag tcggtggcgc
     3421 tgaggctcgt ctgcgagcgc gaaagcgaaa tcgaactttt caaggcgcag gaatattgga
     3481 ccgtcgatgc ggaagtgcgc gcgccggccg gcagcttcca ggcgcacctt acctatctgg
     3541 acggcaagaa agtcgaaaag ctatcgctgg ccaacgaagc cgctgccatg cgcgctgtga
     3601 ccgcgatcga gaagggccgc ttctccatcg gcaatgtcga gagcaagccg gtcaagcgcc
     3661 atccttcgcc gccgttcatc acttcgacgc tgcagcagga agcctcgcgc aagctgggct
     3721 tcgcggccaa acgcaccatg caggtcgccc agcagcttta cgaaggcgtc gatgtgggcg
     3781 gcgaacaggt cggcctcatc acttatatgc gtaccgacgg cgtgacgatg gacggcgccg
     3841 ccatccgtga agcgcgcgag atgatcggac agcgctacgg caagaaatat gtgccgcaca
     3901 gcccgcgcgc ctattcgagc aaggtcaaga atgcgcagga agcgcacgaa gccatccgtc
     3961 cgaccagttt cgcgcgcgcg cccgagttgg tgtcgcgccg cctcgattcc gatcaggcgc
     4021 ggctctacga actgatct
//
LOCUS       DASWPU010000591         5726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_78310,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000591 DASWPU010000000
VERSION     DASWPU010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78310"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1409)
                     /locus_tag="VGC27_12535"
     CDS             complement(84..1409)
                     /locus_tag="VGC27_12535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0283437.1"
                     /translation="MDTPVKLKPSVFNAQIPHNGKVLAFNSLIAFSLEMSEPQARIFN
                     DTLDEIARIGRCEDEAFQRCLYALGFVVPADHDEIAAQQAAFRAAKADADTLRLTIAP
                     TMACNMHCTYCFQQDFAACKTMQPEIERGIIEFVRRKSERSKTLVVQWFGGEPLLRLH
                     RIAAMTEAFQSICAERGMRYYAEMLTNGVLLTPETVASLSKLAVRAIQIPLDGDIETY
                     ARRKGISLQRAQAFHDSLLDSMPSLLEATGSVTIRINVDRNNPAAGYDAVRMFKQKGC
                     CDPRLDFRLGFLNNRDGIVDCIPHDCFSNAEFADTEAAFRRFLAEQGFQVYGEPGPRK
                     YPCAAPLQNNFTISPDGRIGKCVPAIGTDDSVFGQIYPDDIERTMQETAAADAPYSDF
                     DPYLGACSGCEFLPVCLGSCPRSHAPGRSVICALKEGLAETLAFYRVHD"
     gene            complement(1420..2919)
                     /locus_tag="VGC27_12540"
     CDS             complement(1420..2919)
                     /locus_tag="VGC27_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0283438.1"
                     /translation="MQMLNLSEGQYPAVKREFVFKVDGSRPILYSLDYINTQIYYLTP
                     VEGFALSLLNGQRSLSELNGLFTRLLPGFDQPVAEILRGADTLVRSSPSQTGIGKDGL
                     FEVSDHPLADAQAFDPREFVIEPADFAARKSSPKTSLRLDAPINIYTVFTHRCLTDCA
                     YCYADRCKMTEMPLARWREIIGEMRRLGIWLASPDNGDTFARRDGVDLIECLLENEMH
                     FLLSTKAHLGKNTIRRLVDAGFTKKVRGVIQRQVQLSIDAVDDDVSRRVLNIKRSNVG
                     RNLETVENFLSFGIMPKVKAVITGLNYDQPEKIVDRFYERGARVFHFVRYARSFHRHT
                     DDLFVDETAVASLHGQFDKIRDRYDDIEIVENLTADSPLQERLDPETARANWINRIGC
                     GGGWSSLGIAPNGKAFLCEQMKMAEPFFVGDASHQSIEEIWHGAPLMAFIHPDREAFS
                     GTACAPCPEFETCIWEKGRCYRDAYFSYGTAFHPPPLCPSNDRPGLRLS"
     gene            complement(3092..3274)
                     /locus_tag="VGC27_12545"
     CDS             complement(3092..3274)
                     /locus_tag="VGC27_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283439.1"
                     /translation="MAIALDKGKSSEAEREKLVEEIQELIRQKHLDVNLTVGRPVSAT
                     LKGCAQCTICPCMICW"
     gene            3797..5509
                     /locus_tag="VGC27_12550"
     CDS             3797..5509
                     /locus_tag="VGC27_12550"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HEY0283440.1"
                     /translation="MTLLFLSYLGGVLTILSPCILPVLPFVFARADQPFVKSGLPLLA
                     GMALTFAVVATLAAVGGGWAVHANQVGRIAALIVLALFGAMLLLPSLADRFTRPLVAL
                     GDRLGRNTGSGIWASLLLGVATGLLWAPCAGPILGLVLTGAAIQGASVGTTFLLLAYA
                     AGAATSLAVALLIGGRAFAAMKKSLGAGEWIRRVLGALVLAGVVAIAFGLDTGLLTRL
                     SAANTAKIEQSLLDRVRPATSRTVQRVAGSSLPMEGTFPALDGATGWLNSPPLSPASL
                     KGKVVLVDFWTYSCINCLRSLPYTKAWAAKYKGHGLVVIGVHAPEFAFERDPRNVRKA
                     VHDLGVIYPVALDSNLAIWQAFHNEYWPAHYFIDAQGRIRAHHFGEGDYDASERIIQQ
                     LLREAGYRNVPGGIVDPKATGALAAADAGGMLSPETYIGYQRAENFASGPVAPEITSN
                     YRTPADLALNQWGLTGNWTIGAQMAVLDRAPGKIVFRFHARDLHLVLGPSESGKPVRF
                     RVTLDGKPPGNAHGVDTDAAGNGIVTGERLYQLIRQKGTIADHTFAIEFLDPGVQAYS
                     FTFG"
     gene            complement(5496..>5726)
                     /locus_tag="VGC27_12555"
     CDS             complement(5496..>5726)
                     /locus_tag="VGC27_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283441.1"
                     /translation="PNQAFSCGPHALGIQFHPEAVAEKFELWLLGHACEIAGVPGLSV
                     GALRADARRYAQAASEAGALLFSEFLAGISRR"
BASE COUNT         1059 a         1733 c         1802 g         1132 t
ORIGIN      
        1 agcgcacccg atcgctggcg gtcagttgtt ttaccctcat aaaggcattc gccatccttc
       61 acacgaacct cgatgagcgc gaatcaatcg tggacgcgat agaaggcgag tgtctccgcc
      121 agaccttctt tcaaggcgca aatcacgctc cgtcccggcg catgggagcg cggacatgaa
      181 cccaagcaaa ccggcaggaa ctcgcagccg ctgcatgcgc ccagataggg atcgaaatcg
      241 gaatagggcg cgtccgctgc ggcggtctcc tgcatggtcc gctcgatgtc gtccggatag
      301 atttggccga agacggagtc gtccgtaccg atggcgggga cgcatttgcc aatccgcccg
      361 tcgggactaa tggtaaaatt attctgcagc ggcgccgcac aaggatattt tcgcggccct
      421 ggttcaccat acacctgaaa cccttgctcc gccagaaagc ggcggaaggc cgcttccgtg
      481 tcggcgaatt cggcattcga aaagcagtcg tgcggaatgc agtccacaat gccgtctcga
      541 ttgttcaaaa aaccgagccg gaagtcgagc cgcgggtcgc agcagccttt ctgtttgaac
      601 atccgaacgg cgtcgtagcc ggcggctgga ttattgcgat cgacgttgat tctgattgtc
      661 acgctgcccg tcgcctccaa cagcgacggc atactgtcga gcaacgaatc gtggaaggct
      721 tgggcccgtt gcaagcttat gcctttgcgt cgtgcatagg tttcgatgtc gccgtcgagc
      781 ggaatctgga tcgctcggac ggcaagcttc gagaggctgg cgacggtctc gggggtcagc
      841 agcaccccgt tcgtgagcat ttccgcgtaa tatcgcatgc ctctttcggc gcaaatactc
      901 tgaaacgctt ccgtcatggc cgcgatgcga tgcaggcgaa gcagcggctc gccgccgaac
      961 cactgcacga ccagcgtctt gctccgttcc gatttgcgcc gaacgaattc aatgattcct
     1021 cgctcgatct cgggctgcat cgtcttgcat gctgcgaagt cctgctggaa gcagtaagtg
     1081 caatgcatgt tgcacgccat cgtcggcgca atcgtgagcc gcagcgtgtc ggcatcggcc
     1141 ttggccgccc ggaaggcggc ctgttgcgcg gcgatttcat cgtggtcggc gggcaccacg
     1201 aagccgaggg cgtagaggca gcgctgaaac gcttcgtcct cgcaccggcc gatacgggcg
     1261 atttcatcca aagtgtcgtt gaaaatgcgg gcctgcggct cgctcatctc cagcgaaaag
     1321 gcgatcaagc tattgaaggc aagcactttg ccgttgtgcg gtatctgcgc attgaaaacg
     1381 gatggcttca atttgacagg cgtgtccatg acggtgtcct caactcaagc gcagtccagg
     1441 gcgatcattg ctggggcaaa gcggcggcgg atggaacgcc gttccatacg agaaataggc
     1501 gtcgcgatag cagcggcctt tttcccagat gcaggtctcg aactccgggc agggcgcgca
     1561 tgcggtgccg gagaacgcct cgcggtcagg atggatgaag gccatcagcg gcgcgccgtg
     1621 ccagatttct tcgatcgatt gatggctcgc gtctccgacg aagaacggtt ccgccatctt
     1681 catctgttcg cacaggaatg ccttgccgtt cggcgcaatg ccgagcgagg accagccgcc
     1741 gccgcagccg atgcggttga tccagttcgc tcgggcggtc tccggatcga gcctctcctg
     1801 caaaggagag tcggccgtga gattttcgac aatctcgatg tcatcgtacc ggtcgcgtat
     1861 cttgtcgaac tggccgtgga gcgaggccac ggcggtttcg tcgacgaaca ggtcgtcggt
     1921 atggcgatgg aaagaacggg cgtagcgcac gaagtggaaa acgcgggcac ctctttcgta
     1981 gaaccggtcg acgatcttct cgggctggtc gtagttgagg cccgtgatga ccgccttgac
     2041 cttcggcatg attccgaatg ataggaagtt ctcgaccgtt tccagattgc ggccgacgtt
     2101 cgaccgtttg atgttcagga cgcgtcggga cacgtcgtcg tcgaccgcgt cgatgctcaa
     2161 ctggacctgc cgctggatca cgccgcggac cttcttggta aatccagcgt cgaccagacg
     2221 ccggatcgtg ttcttgccga ggtgggcctt ggtcgataac aggaagtgca tttcattctc
     2281 gagcaggcac tcgatcagat cgaccccatc gcggcgggca aaggtgtcgc cgttgtccgg
     2341 ggaggccagc caaatgccca gacgccgcat ttctccgatt atctcgcgcc agcgggcgag
     2401 cggcatctcc gtcattttgc agcgatcggc gtagcaatag gcgcaatcgg tcaggcagcg
     2461 atgggtaaaa accgtgtaga tgttgatcgg cgcgtcgagc cgcagtgagg tcttcgggct
     2521 gctcttgcgg gcggcaaaat ccgccggttc gatgacgaac tcgcgcggat cgaaggcctg
     2581 tgcgtcggca agcgggtgat cggacacctc aaacaatccg tccttcccga ttcccgtctg
     2641 cgatggagag ctgcggacga gcgtgtcggc gccgcgcagg atttcggcca ctggctgatc
     2701 gaagcctggc aacaacctcg tgaacaatcc gttcagctcc gacagggatc gctgcccgtt
     2761 gagcaaggac agcgcaaatc cctcgaccgg tgtgaggtag tagatctgcg tgttgatgta
     2821 gtcgagcgag tagaggatcg gtcgggaccc gtcgaccttg aaaacgaatt cgcgtttgac
     2881 ggccggatat tggccttccg acaaattgag catctgcatg atgtcttccg atcgatcttt
     2941 cggcgccgtg atggcggcaa cgcggaaacg ccgtcaatgc gaggtcacgg gtggacgttt
     3001 tgacgaagga gagccgacgg ccccaatttt tcgccatccg cgctacgcac ggatcgcgat
     3061 gaaagcgtgg ccgccggctc gtccgcgacc gtcaccagca aatcatgcag gggcagatcg
     3121 tacactgcgc gcaacccttc agagtggccg aaaccggccg cccgaccgtg aggttcacgt
     3181 cgaggtgctt ctgccggatc agttcctgta tctcttcgac cagcttctcg cgctcggcct
     3241 cggatgactt acccttgtcc aatgctattg ccattgtttc ctccattggt ggacggaaaa
     3301 acctacgagg cacttgcgaa tgcacgcaac tgcacagacg tttgcgcgca tgacaaacgt
     3361 aacagggcgg cggacccggt ccgggctcgg ctgaattgtc ccctacggcc ttgcgtggcg
     3421 tgcggcaggt gataaagcgg tggccgatgc aagcaatgac agcgtgacga gagaaagcgg
     3481 gaggcacgac caggtagagc gtggtgggtg acggtccttc ggccagatcg gcgatgcgcc
     3541 agtcacggcg gcgcgtgacc cgcgccacca ccgggtcgcg ataaaggccc atgcttgtcg
     3601 agcgaggcga acaagggctc gcccgagcct ttaaatccgg gttaacctcc tatttaggtt
     3661 ggccgccccc aaataccaga gatgcgtgcg aaaacagccg cttgaccacg cctgacagat
     3721 cgagtcccgg ctgacgctgt aactgtccta ccggttcgtg cgaatatacc cgggccattc
     3781 ggacgaagtt ctgtcgatga cccttctgtt cctttcctat ctcggcggcg tactgacgat
     3841 cctcagcccc tgcatcctgc cggtgctgcc ctttgtgttc gcgcgcgccg accagccctt
     3901 cgtcaaaagc ggattgccgc ttctggccgg aatggccttg accttcgctg ttgtcgccac
     3961 gctggcggcc gtcgggggcg gctgggccgt ccacgccaat caagttgggc gcatcgccgc
     4021 gctgatcgtt ctggcgctgt tcggcgcgat gctgcttctg ccttcgctcg cggaccggtt
     4081 cacacggccg ctagtggctt taggcgaccg gcttggccgc aacacgggca gcggtatctg
     4141 ggcatcgctg cttctgggtg tcgcgacagg gctgttatgg gcgccttgcg ccgggccgat
     4201 tttgggcctc gtgctgacgg gcgccgcgat ccagggcgcg agcgtgggga cgacgttcct
     4261 gttgctcgct tacgcggccg gcgcggcgac gtcgctcgcg gttgctttgc tgatcggcgg
     4321 gcgcgccttc gcggcgatga agaaatcgct cggcgccgga gaatggatcc gccgcgtgct
     4381 gggcgcgttg gtgctggccg gcgtggttgc catcgccttc ggattggata cgggactcct
     4441 cacgcggctt tcggcggcca acaccgcgaa gatcgaacaa tcgttgctcg acagggtgcg
     4501 gcccgcgacc agtaggacgg tgcagcgcgt ggctggaagc agcctgccca tggaaggcac
     4561 tttccccgcg cttgatgggg cgaccggctg gctcaactcg ccgccgctct cgcctgcgtc
     4621 gctcaagggc aaggtcgttc tggtcgattt ctggacctat tcctgcatca actgcttgcg
     4681 ttcgctgccc tacaccaaag cctgggccgc gaagtacaaa ggccatggcc tggtcgtgat
     4741 cggggttcat gcgccggaat tcgccttcga acgcgatccc cggaatgtgc gcaaagccgt
     4801 gcacgatctg ggcgtcatct atcccgttgc gctcgacagc aatctggcga tctggcaagc
     4861 gttccacaac gaatattggc ccgcgcatta tttcatcgac gcgcaggggc gcatccgcgc
     4921 gcatcatttc ggcgagggcg actacgacgc ctcggagcgc atcatccagc agcttctgcg
     4981 cgaggccggc taccgcaatg tgcccggcgg catcgtcgat ccgaaggcga ccggcgcgct
     5041 cgccgcggca gatgccggcg gcatgctctc gcccgaaacc tatatcggat accagcgggc
     5101 tgagaatttc gcgtcgggtc ccgtcgcgcc ggagattacg tcaaactaca gaacgcccgc
     5161 cgatctggcg ctgaaccaat gggggctgac cggaaactgg accatcggcg cgcagatggc
     5221 ggtccttgat cgcgcgcccg gtaagatcgt cttccgcttt cacgcccgcg atctgcatct
     5281 cgtgctcggc ccgtcggaga gcggaaagcc ggtgcgcttc cgggttacgc tggatggcaa
     5341 accgccgggt aacgcgcatg gcgtggatac ggacgcggcg ggtaacggca tagtgaccgg
     5401 cgagcggctc tatcaactca ttcgccaaaa agggaccatc gccgatcata ccttcgcgat
     5461 cgaatttctc gatccgggcg tgcaggccta ttccttcacc ttcggctgat gccggccagg
     5521 aactcggaaa acaacagcgc tcccgcttcc gatgccgctt gtgcatagcg gcgcgcatct
     5581 gcgcgcagag cgccgaccga gaggcccggc acgcctgcga tctcgcaggc atggccgagc
     5641 agccaaagct cgaacttttc tgccacggcc tcgggatgga attgaatacc caaggcatga
     5701 gggccgcagg aaaaggcttg gttcgg
//
LOCUS       DASWPU010000592         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_67016,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000592 DASWPU010000000
VERSION     DASWPU010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67016"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1937
                     /locus_tag="VGC27_12560"
     CDS             <1..1937
                     /locus_tag="VGC27_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="exopolysaccharide transport family protein"
                     /protein_id="HEY0283442.1"
                     /translation="QRKNNVADQSSVLSALPTDAPSVQNQIQVLSSRELAERVIGKLK
                     LYDDPEFNPALNGNAAPGIGDVVRLLNPANWGAPRPGAGAGYQRDAIVNAFLNRLDVS
                     ALGLSTSITVTFTSRDPAKAARIANALADAYTEDQVAIKVSAARKATQWLTDRMHQLA
                     QQVQAGESAIAQYKAEYNLVDSAQGSSLVDQQLVAINALLIAARSDLAEKKANYDRVQ
                     AMVRSGNTVDVSQVVASPLIVQLRSQEGYLIRQEADLSARYGPNHPKMIAIRTQKRDL
                     ENKVAQEAARVADSVASDLAVARAHVGSIQASLAQVEKQAAGDDISRIKLNALQANLA
                     STRTMYESFVTRLRATQDQDDIQNPESRVISRAPIPNAPSSPHRLLFFAASLPAGLLL
                     GVLLALLAERFQPPPPRPGRRPFEPAAPATAKPAPALPPILGEIQATSDMRAADFVVD
                     NPGAPYSRALSSLLSRMTPARAGRGRVVALTALGSDAGRAVIALGLARVAASQGLRTV
                     LVDGDFTRPAIAPAVGYRSVPAGIVELLDGSAPLSLALLKDPRSAAFVLSAARPTGAA
                     EALWTSPKMAELLNYFRQNCDFVIIAAAPALTDMPFLARLCDQVVLIAPGQAPQAVLE
                     TAARTLTACRAPQAGLIVTR"
     gene            2115..>2341
                     /locus_tag="VGC27_12565"
     CDS             2115..>2341
                     /locus_tag="VGC27_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283443.1"
                     /translation="MSETPSMLTRMASAVGNLPPAAKNAGILIAVLVVGGLAGWIGHG
                     QLAGPADVPTMTVHQEWRLLCPALKDKDVSC"
BASE COUNT          397 a          769 c          744 g          431 t
ORIGIN      
        1 atcagcgcaa gaacaacgtc gccgaccagt cttcggtact gtcggcgtta ccgaccgatg
       61 caccctcggt gcagaaccag atccaggtcc tgtcttcgcg cgaactggcc gagcgcgtca
      121 tcggcaagct gaagctttac gacgacccgg aattcaatcc ggccttgaac gggaacgccg
      181 caccgggcat cggcgatgtc gtgcgcctgc tcaatccggc gaattggggc gcgccgcgtc
      241 cgggggcggg cgccggctat caacgcgatg cgatcgtcaa tgcttttctc aaccggctgg
      301 atgtcagcgc gttgggtcta tcgacctcga ttaccgtgac tttcacctcg cgcgatcccg
      361 ccaaggccgc ccggatcgcc aatgcgctgg cggacgccta caccgaagat caggtcgcca
      421 tcaaagtctc cgccgcgcgc aaggcgacgc aatggctgac cgaccgcatg catcagttgg
      481 cgcagcaggt tcaggccggg gaaagcgcga tcgcgcaata caaggccgag tacaatctgg
      541 tggactcggc gcaaggcagt tcgctggtcg atcagcagct cgtcgcgatc aacgccctct
      601 tgattgcggc gcgatcggat ctggcggaga agaaggccaa ttacgaccgc gtccaggcga
      661 tggtgcgttc gggaaacaca gtggacgtat ctcaggtcgt tgcctcgccg ctgatcgttc
      721 agttgcgttc ccaggaaggc tacctcattc gtcaggaggc cgacctttcg gcgcgctacg
      781 gccccaacca tcctaagatg atcgcgatcc gcacgcagaa gcgcgatctg gaaaacaagg
      841 ttgctcagga agcggcccgc gtcgcggact cggtcgccag cgatctggcg gtcgcccgtg
      901 cgcatgtcgg ttcgatccag gcgagccttg cccaggtcga aaaacaagcg gccggagacg
      961 acatctcgcg catcaagctg aatgcgctcc aggccaatct cgcctcgacg cgcaccatgt
     1021 acgaatcttt cgtcacccgg ctgcgcgcga cccaggacca ggacgacatc caaaatcccg
     1081 agtcgcgcgt catttcccgg gcgcccatcc cgaacgcgcc gtcgtcaccg caccgtcttc
     1141 tgttcttcgc cgcgtccttg ccggcgggat tgctgctggg cgtattgctg gcgctgctgg
     1201 ccgagcggtt tcagccgccg ccgccccggc cgggccgccg ccccttcgaa ccggcggcac
     1261 cggccacggc aaagccggcg ccggccttgc cgcccatcct gggggagatt caggccacct
     1321 cggacatgcg cgcggccgat ttcgtggttg acaaccctgg tgcgccttac agccgggctt
     1381 tgtcctcgct cttgagccgc atgacaccgg ctcgcgccgg ccggggcagg gttgtggcgc
     1441 tgactgcgct cggctccgac gccggacggg cggtgatcgc gctgggcttg gcgcgcgtgg
     1501 ccgccagcca gggcttacgg acggtcctgg tggacggcga cttcacccgg cccgccatcg
     1561 ccccggcggt gggataccgt tccgttccag cgggtatcgt cgagctgctg gacgggtcgg
     1621 cgccgctgtc gcttgctctg ttgaaggatc caagatcggc cgcattcgtg ctctccgccg
     1681 cacggccgac gggcgcggcc gaggcgctgt ggacgtcgcc caagatggcg gaactgctga
     1741 attattttcg gcagaattgc gattttgtaa tcattgccgc cgcacccgcg ctgacggaca
     1801 tgccgttcct ggcgcggctc tgcgatcagg tggttctgat cgcgccgggg caggcgcccc
     1861 aggcggttct ggaaactgcc gcgcgcacgc tgaccgcttg ccgggcgccg caagccggtc
     1921 tgattgtgac ccggtaggac gctgtcgcgc ttgacttttc gttgcggggg cagttcatac
     1981 gcgccacgtt cagggtgacc atcctgcaaa gatttaattg agcgttccgc gaaaagttga
     2041 gatacgcgga acaaaagatt gtgtccttct cggatgcggc caaatttcac ttacggtagc
     2101 aaagcgaacc ccacatgagc gaaacgcctt ccatgctgac gcgtatggcg agtgccgtcg
     2161 gcaatctgcc tcctgcggcg aagaacgccg ggattctcat cgccgtgctt gttgtcggcg
     2221 gtcttgccgg ctggatcggc cacggccaat tggccggacc cgccgatgtt ccgaccatga
     2281 ccgtccatca ggaatggcgt ctgctctgtc cggcgctcaa ggacaaagac gtgtcctgcg
     2341 a
//
LOCUS       DASWPU010000593         3940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_48102,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000593 DASWPU010000000
VERSION     DASWPU010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48102"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VGC27_12570"
     CDS             <1..321
                     /locus_tag="VGC27_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY0283444.1"
                     /translation="MAVVPIDAVYVDANFKEGQLGKVRPGQPATLTSDLYGSAVKFHG
                     RVAGIGGGTGSAFAVIPAQNATGNWIKVVQRVPVRIALDPAELAKHPLRVGLSMNVTV
                     DLSE"
     gene            328..1887
                     /locus_tag="VGC27_12575"
     CDS             328..1887
                     /locus_tag="VGC27_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0283445.1"
                     /translation="MSSIAAGWAGPPPAKAEPSSLMLVIIVFVLAMANFLAILDLTIV
                     NVLVPHISGALAVAPSDGTWVITAYAVAEAITVPLTGWLAERFGPVRVFVVAIAGFGI
                     FSVLCGMATSLPMLIAFRVALGVCGGPLIPLSQTLLLQIVPPRHEITALAAWSMTTII
                     APIVGPALGGLIGDNWGWPWAFYIKVPLAAVLAFAAWWALTPHETPARKIPVDYIGLS
                     LLILWVGALQIMLGNGQNMDWFNSDFIVMLFVTTIIGFLAFVVWEMTEKTPIVDLRIF
                     SNRAFSVSMVVIALSFGALFGSVVLVPLWLQTAMGYTATLAGYNSAFAGVFSVIAAPL
                     TVLMMKHLDHRAVVTIGLFFCAISSLVRVFFTQDMTFEQLLWPQVAQGLGFPMIVIPL
                     MDMSVSSLPPKDTASGAGQFNFIRTLSSAVSTAVVVAVWIDAINVNKAALAGQLHNPQ
                     AVLDRMQAGGFSADRALHLFDLLVQGQAVMLGTNSTFLAVGIVTLVAAAVVWVAPKPP
                     RQGNGKPGLGH"
     gene            1963..2313
                     /locus_tag="VGC27_12580"
     CDS             1963..2313
                     /locus_tag="VGC27_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283446.1"
                     /translation="MKDEDFSAERMAEISLAKLFRHRVDSEHLRRVASWLGLLILGVE
                     KIKDRWWVSHSKQLCFEFDGRRFEARYNHRIEPHGGIEIVEVEPKSGKSDISVAKVIA
                     NLKDAAKFFRSPRL"
     gene            complement(2315..3658)
                     /gene="hemN"
                     /locus_tag="VGC27_12585"
     CDS             complement(2315..3658)
                     /gene="hemN"
                     /locus_tag="VGC27_12585"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEY0283447.1"
                     /translation="MRTPADLFSARVPRYTSYPTAPHFHAGVTGEIFREWLAALPQGR
                     LSLYLHVPFCDTLCWFCGCHTKIVNHYSPLASYLELLLNEIKTVSAVIGPGHPVTHIH
                     WGGGSPTMLSPGDVKRLADTLKAHFEIAPGAEFAIEIDPRGLENEMIAALAEAGVNRA
                     SIGVQDCDETVQRAINRIQSIEVTRSAVERLRAAGIGALNIDLIYGLPHQTQANVART
                     IDMALTLTPQRFAVFGYAHVPHFKKHMRLIDEKALPGAAERLAQFELAHAKLCASGYA
                     AIGLDHFALPEDSLALAQTAGTLARNFQGYTTDDAPALIGLGASSISALPGGYAQNKA
                     EVPEYRQAILAGELPVARGVQLSGDDRLRRAIIERLMCDLEVDLDAVAAPFGKSAADF
                     KPELAALASYAEQGIVAIEGSRIAVPPEARAAVRLVAAAFDRYLGKSQAVHAVAV"
     gene            complement(3659..>3940)
                     /locus_tag="VGC27_12590"
     CDS             complement(3659..>3940)
                     /locus_tag="VGC27_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283448.1"
                     /translation="GKERGRQGGLIKARALSMTAAPYQGLGTWLQCRPALEEGPSLRL
                     ICSQGARHEDHPPELQNGQGNMEHITVYRYCDAWLRDINDSAAAASITG"
BASE COUNT          670 a         1257 c         1234 g          779 t
ORIGIN      
        1 atggcggtgg ttcccatcga cgccgtctat gtcgatgcca acttcaagga aggtcagctc
       61 ggtaaggtac gccccggtca gcccgcgacc ctgaccagcg atctctacgg ctcggcggtg
      121 aagttccacg gccgcgtcgc gggcatcggc ggcggcaccg gttccgcctt cgccgtgatc
      181 ccggcccaga acgcgaccgg gaactggatc aaggtggtgc agcgcgtccc cgtgcgcatc
      241 gcgctcgacc cggcggaatt ggccaagcac ccgctgcgcg tcggcctgtc gatgaacgtc
      301 accgtcgacc tttcggaata agatcgcatg tcgagcatcg ccgccggttg ggcagggccg
      361 ccgcccgcga aagcggaacc gagcagcctc atgctggtca tcatcgtgtt tgtgctggcg
      421 atggccaact tcctggccat tctcgacctc accatcgtca atgtgctggt tcctcatatc
      481 tccggcgcgc tggccgtggc ccccagcgac ggaacctggg tgatcaccgc ttatgcggtg
      541 gcagaggcga tcaccgtgcc gctgaccggc tggctggccg agcgtttcgg gccggtgcgc
      601 gtcttcgtgg tggctatcgc cgggtttgga atcttctccg ttctctgcgg catggcgacg
      661 tcgctgccta tgctgatcgc cttccgcgtg gcgctggggg tctgcggcgg tcccttgatc
      721 cccttgtcgc agaccttgct gctgcagatc gtgccgccgc gccatgagat caccgccttg
      781 gcggcctggt cgatgaccac gatcatcgct ccgatcgtcg ggcccgcgct tggcggtctg
      841 atcggcgaca actggggctg gccctgggct ttctatatca aggtgccgct cgccgcggtg
      901 ctggccttcg cggcctggtg ggctttaacg cctcacgaaa cgccggccag gaaaattccg
      961 gtcgattata tcgggctcag tctcctgatc ctgtgggtcg gcgcgctgca gatcatgctc
     1021 ggcaacggcc agaacatgga ctggttcaat tccgacttca tcgtcatgct gttcgttacc
     1081 acgatcatcg gctttctggc tttcgtcgtc tgggagatga ccgagaagac ccccatcgtg
     1141 gatctgcgca ttttcagcaa ccgcgccttc tcggtgtcga tggtggtgat cgcgctgtcc
     1201 ttcggcgccc tgttcggctc ggtcgtgctg gtgccgctgt ggctgcagac cgcgatgggc
     1261 tacacggcga ccctggcggg ctacaactcc gccttcgcag gcgttttctc cgtcatcgcg
     1321 gcgccgctga cggtcctgat gatgaagcac ctcgatcatc gggcggtggt gacgatcggg
     1381 ctgtttttct gcgcgatttc ctcgctggtg cgcgtgttct tcacccagga catgaccttc
     1441 gagcagcttc tctggccgca agtcgcccaa ggactgggtt tcccgatgat cgtcattccc
     1501 ttgatggata tgtcggtaag ctcgttgcct cccaaggaca ccgcctcggg cgccggccag
     1561 ttcaacttca tccgcacctt gtcgagcgcc gtctccaccg ccgtcgtcgt cgccgtctgg
     1621 atcgacgcca tcaacgtgaa caaagcggcg ctggccggcc aacttcacaa tccgcaggcc
     1681 gtcctcgacc ggatgcaagc cggcggcttt tccgccgacc gggcgctgca cctcttcgat
     1741 cttctggtgc aaggacaggc cgtgatgctg gggacgaact ccaccttcct ggccgtcggc
     1801 atcgtcacgc tggtggcggc ggccgtggta tgggtggcgc cgaagccgcc gcgccaaggc
     1861 aacggcaaac cgggattggg acattagcgt cctgcccgcc ggggcgcaga agaatttcgt
     1921 caaatatgag ctatatagat acggcagcct taggagggtc atatgaaaga cgaggacttt
     1981 tcggccgagc gcatggccga aatatcgctg gcaaagctct ttcgtcaccg ggtggacagc
     2041 gagcatctgc gccgcgtcgc ttcgtggctg ggactgctca tccttggcgt cgagaagatc
     2101 aaggacagat ggtgggtgtc ccactcgaag cagctttgtt tcgagttcga cggaaggcgg
     2161 ttcgaagcgc gatacaatca ccgcatcgag ccgcacggcg gcatcgaaat cgtcgaggtg
     2221 gaacccaaat ccggaaaatc cgacatcagt gtcgccaagg tgatcgcaaa cctgaaagac
     2281 gcggccaagt tcttccgctc gccccggctg taaatcaaac cgctacggcg tggacggctt
     2341 ggctcttgcc caagtaacgg tcgaaggcgg cggcgaccag cctgacggcg gcgcgcgctt
     2401 ccggcggcac ggcgatccgc gaaccttcga tggcgacgat gccctgctcc gcatacgaag
     2461 cgagcgccgc aagctccggc ttaaaatccg ccgccgattt gccgaacggc gccgctaccg
     2521 catcgaggtc cacctcgaga tcgcacatca gccgttcgat gatggcgcgg cgcaggcgat
     2581 cgtcgccgga aagttggacc cctcgcgcga ccgggagttc gccggccagg atcgcctggc
     2641 gatattccgg aacctcggcc ttgttctgcg catagccgcc cggcaaggcg ctgatcgaag
     2701 acgctcccaa tccgatcagg gcgggcgcat cgtcggtggt gtagccctgg aaattgcgcg
     2761 ccagcgttcc ggccgtctgt gccagcgcca gcgaatcctc cggcagcgcg aaatgatcca
     2821 gcccgatggc ggcatagccg ctggcgcata gtttggcgtg cgccagttcg aactgcgcca
     2881 gccgttctgc ggcgccgggc agcgcctttt cgtcgatgag ccgcatatgc ttcttgaaat
     2941 gcggcacgtg agcgtaaccg aacaccgcga aacgctgcgg cgtcagcgtc agcgccatgt
     3001 cgatggtgcg cgcgacgttc gcctgcgtct ggtgcggcag tccgtagatc agatcgatat
     3061 tgagcgcgcc gatgccggcg gcgcgcagcc gttccaccgc gctgcgtgtg acctcgatgg
     3121 actggatgcg gttgatggcg cgctgcacgg tttcgtcgca atcctgcacg ccgatgctgg
     3181 cgcggttgac ccccgcctcc gccagcgccg cgatcatctc gttctcaagg ccgcgcggat
     3241 cgatctcgat ggcgaattcg gcccccggcg caatctcgaa atgcgctttg agcgtatccg
     3301 ccagcctctt cacgtcgccg ggcgagagca tggtcggcga gccgccgccc caatggatgt
     3361 gcgtgacggg atggcccggg cctatgacag cgctgacggt tttgatctcg ttcaacagca
     3421 actcgagata ggacgcgagc ggcgaatagt gattgacgat cttggtgtgg caaccgcaga
     3481 accagcatag cgtgtcgcaa aacggcacat gcagataaag cgacagccga ccctgtggca
     3541 atgctgccag ccattcgcga aaaatttcgc ccgtcacgcc ggcatgaaaa tgcggcgcgg
     3601 tcggatagct cgtgtagcgc ggaacacggg cggaaaaaag atcagcaggc gtacgcattt
     3661 atcccgttat ggaagcggct gcggcgctgt cattgatatc tcgcaaccac gcatcgcaat
     3721 agcgataaac cgttatgtgc tccatattgc cttgaccatt ttgaagttcg ggaggatgat
     3781 cttcgtgcct cgctccttga gagcagatga ggcgcaaaga cggaccctcc tcgagggccg
     3841 gtcggcattg gagccaagtg cccaggccct gatagggcgc tgccgtcatc gacagcgccc
     3901 gcgcctttat gaggccgcct tgcctccccc gctcctttcc
//
LOCUS       DASWPU010000594        14574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_115882,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000594 DASWPU010000000
VERSION     DASWPU010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 14574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14574
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115882"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VGC27_12595"
     CDS             complement(<1..513)
                     /locus_tag="VGC27_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283449.1"
                     /translation="MAATDIDLVAGRACGECTVCCTVMAIDKPEIQKEAGLTCRHCAR
                     GCTIYETRPALCRDFYCGWRQLPILDDSWRPDRSGVFVELEQIDGQTGLSLVLIGNPL
                     KTVRQTWFIEFVAVGVREDVPLQLGIPGPRGHQGASLLLNTSQMLEAARTSRLRVKEM
                     LEKELKRLRSY"
     gene            complement(524..1174)
                     /locus_tag="VGC27_12600"
     CDS             complement(524..1174)
                     /locus_tag="VGC27_12600"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY0283450.1"
                     /translation="MNAFANTFLLCYAALFPIVNPVGNIPLFLGLTRSRTHRERNALA
                     GRVAINSFFLLLASLLVGSHVLEFFGISLPVVRIGGGLVVTAFGWHLLNSGASVDGDA
                     AAAGPAPKPLDTFYPFTMPLTVGPGSIATAIALGSQRPSLAGLPELVLLGLAAIAGLL
                     AISATIYLCYRFAEGTISRLGPGGTNVVVRLSAFVLVCIGIQIIWTGWTGLMALPR"
     gene            complement(1241..2269)
                     /locus_tag="VGC27_12605"
     CDS             complement(1241..2269)
                     /locus_tag="VGC27_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY0283451.1"
                     /translation="MRRRRTIWIVLVGLLAAAFAAAGQAQPPRLSFQVATGSTAGTYF
                     PMGELIAGIVSHPPGLARCRARGVCGPPGLIVSARTSEGAVGNVLAVNAGGVDSGLAQ
                     ANVVADAVAGRGAFRKAGRQSHIRVIANLFPETVQLVVAAKSPIAKVADLSGKRVSLG
                     ADGSGAAVVAREILAAYRVPMKRLKLRNESYDVAAGLLQQGKLDAFFFLGGAPSPLVG
                     DLIARGTAKLIAIDGKARDRVLLRVPSLSAGVIPAGAYRGTGAIQTVSSRTLWVVKDS
                     ASPDVVYGILRALYHPANRALFDKGAPSARYIRLDTATDGLTVPLHPGAERFYRDMGK
                     LAVPPPSH"
     gene            2402..3277
                     /locus_tag="VGC27_12610"
     CDS             2402..3277
                     /locus_tag="VGC27_12610"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0283452.1"
                     /translation="MHRLLVRLGALALSTAALAACASDPAIPSLKQAKNDAAAGTTPS
                     LEGDIRTAQVQRASGDYTGAIRSLSQLMLVAPDDPRVVGEYGKVLVQQGRGPEALNFL
                     KRAIELQPGDWTLYSALGVTYDQIKDPVKARDAYQRALVLKPGEAAVLNNFALSRMLA
                     GDPAQAKTLMAQAAASGSNEPKIAHNLALIDGLAGPKAPPAVQSDAPAATPQATTAPA
                     AEHPPTPLSMAKSDGTAGPTTTFMQQVPYDPQAGPVGKPAKAAPRKLAKAPLKTPAET
                     VKSTIPALRLANDNP"
     gene            complement(3458..4441)
                     /locus_tag="VGC27_12615"
     CDS             complement(3458..4441)
                     /locus_tag="VGC27_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0283453.1"
                     /translation="MFNSEIVNLLFDPKFLATAAVGILAFATIVTLGLPLLERDTLGR
                     RLKAVAERREELRARHHAQLNARRGALRSEPVGTTKLIKQIVERFKLGKLVESEDSRD
                     KLATAGLRSQTHHVVFMFFRFVMPFILFALALFYLFVVTHFAWSLTLKMAIAVAAALF
                     GFYLPDLFVTNLTTRRQTSIMNAFPDALDLLLICVESGMSVEAAFAKVASEIGSASEE
                     LAEEFALTTAELSYLPDRKQAFENLAKRCGHPGVKQVATALNQAEKYGTPMGQALRIC
                     AQENREMRMAAAEQKAAALPAKLTVPMILFFLPCLFVVIMGPAVMKVMHMM"
     gene            complement(4452..5417)
                     /locus_tag="VGC27_12620"
     CDS             complement(4452..5417)
                     /locus_tag="VGC27_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0283454.1"
                     /translation="MVMLAVALLVLAFGGAAFAVAFGSNETSQKRIAVIAKAQPRAAR
                     GAAESQGQRRKGMQQLLKEIEKKQAEKKPTMTLRRRLDRAGFTDVPPRNFWIASGLLA
                     FVVAALCLYTQQSLLITALASFGTGFGLPRWVLNFLRKRREKRFTNDFAGAIDVIVRS
                     VKSGLPTGDALRIVAGEFRDPVGSEFTRLVDSMKVGVTLEQGLRRMYENMPTTEVSFF
                     AIVMTIQQKSGGNLSEALGNLAAVLRDRKRLQGKIKAMSSEAKASAMIIGSLPPGVMA
                     LVYFTSPAYMTLLFTERVGNLLLAGCVLWMGLGIFVMKKMINFKH"
     gene            complement(5423..6868)
                     /locus_tag="VGC27_12625"
     CDS             complement(5423..6868)
                     /locus_tag="VGC27_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY0283455.1"
                     /translation="MFGKRTATDSLAPPPRAEAKAARPPPKAEGTGARELAAAPPPAI
                     AAPPQHTFVADERSDDYYQVKTTIFSALIDTIDLAQLAQLDADSAREEIRDIVNEIIS
                     IKAVVMSIAEQEHLLQDICNDVLGYGPLEPLLARDDIADIMVNGAGRVFIEVAGKVQL
                     TPIRFRDNAQLMNICQRIVSQVGRRVDESSPICDARLLDGSRVNVIAPPLSLDGPTLT
                     IRKFKKDKLRMDDLIRFGSINEAGAKLLGIIGRCRCNVLISGGTGSGKTTLLNCMTAY
                     IDVEERIITCEDAAELQLQQPHVVRLETRPPNLEGQGQITMRDLVRNCLRMRPERIIV
                     GEVRGPEAFDLLQAMNTGHDGSMGTLHANSPREAMNRLESMITMGGFQLPTRTVREMI
                     VGSIDVIIQGERLRDGTRRITKITEVVGTEGDVVITQDLLTYEMSGEDEGGRIKGRHI
                     GTGIVRPAFWERVRYYGLERELAEALDKLQE"
     gene            complement(6872..8119)
                     /locus_tag="VGC27_12630"
     CDS             complement(6872..8119)
                     /locus_tag="VGC27_12630"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0283456.1"
                     /translation="MRHEENRPAFGAEAHERPVPRISIHAFCEFPDTGAAMQRAGADR
                     RLAKANLSVQLGGIAAAIEHYAGQVTPNLLIVETRLSGEQALGELDSLAEVCDAATKV
                     VVIGRVNDVELYRELMRRGASEYLVAPVDPLHLIEVIAGLYSDPEGHPIGRVIAFVGA
                     RGGAGSSTLCHNVGWCIAEEQHINTTILDFDLAYGTLGLDFNDEAGQSIADALTAPER
                     LDEVLLERLLIRRGDYLSLFAAPAILEREYDAGLDAYESVIDAVRQTTPCVLVDLPHS
                     WEPWVKATLIGADDIVVVATPDLASLRNAKNLVETLRRNRPNDAAPRLVLNQVGQPKR
                     PEIPAKDFAETFGLEPSIVLPFDAALFGQAANNGQMLAELGQRSPAVEAVRRLAGLVT
                     GRKQANEKSPASLFSLLKAKKRA"
     gene            complement(8143..8811)
                     /locus_tag="VGC27_12635"
     CDS             complement(8143..8811)
                     /locus_tag="VGC27_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HEY0283457.1"
                     /translation="MKNLLRYASVAAMLLAGSCASPLNQYTLSNDGAANHPITVEAHY
                     NSIKLPFSAPAAGLLPDDAARLDEFVARYLERGNGALSISAPAGRDASAAIGYFGERL
                     ASLGVDRSRIMVGTHQAENGDSRVEIGFVGYVARTDPCGDWSKDLSDTAANQSSPNFG
                     CATQHNIAAMVADPRDLVQPRPMAQSDAVRRGIVVGKYEKGEVTAADKSQDQSGKVSG
                     VVGQ"
     gene            complement(8821..10311)
                     /locus_tag="VGC27_12640"
     CDS             complement(8821..10311)
                     /locus_tag="VGC27_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEY0283458.1"
                     /translation="MHKYLLPVFGIAAALILVAADPVFAAPRADEDGAARVITVATRS
                     NGPVHQRLVLPQDKAAIVQIDADARDVLVSNPEIVDAVVRTPRRIFLLAIKNGQTNAF
                     FFDATGRQLLSLDIRVEKDVTDLNRLMRTELPGSGVKASAMNDNIVLTGKVASAQAST
                     RAQDIAARFAGDPAKVVNMLQISGSEQVMLKVRIAEVQRQVAKQLGIDLSGAAVINGV
                     PLIASTSNPYGLMGQALSDLSGARIGMTCTPTAAGTCLGGPNNVQGAMKALETVGLVH
                     TLAEPNLTAVSGETAKFLAGGEFPVPSGRDTQGNVSITFKQFGVGLSFTPVVLSSGRI
                     SLQLSTEVSELTNTGAFTLQGGTSVDPNGNPVTVGATTIPALSVRRTETTIELPSGGS
                     FAVAGLMQHTTRQQLDGFPGLKDMPILGALFRSRDFQNNETELVVMVTAYLVSPTTEA
                     KLAAPTDGFLPPTDPETILLGRLNETYSRGKRPVSQAQGLTGFIVQ"
     gene            complement(10312..11112)
                     /gene="cpaB"
                     /locus_tag="VGC27_12645"
     CDS             complement(10312..11112)
                     /gene="cpaB"
                     /locus_tag="VGC27_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY0283459.1"
                     /translation="MNTRRLVVILLAAVAAGGAALLVRGLIGGGTTKADARLQPAPIA
                     LSNVLVAASNLQPGQPLSAGLVRWQKWPTSSVDPGFIQQTGALSADAAVAGTVARAPM
                     VAGEPVTYAKIVRSDSSGFMAATLQPGMRAVSIPVSIASVAGGFILPNNHVDIILTET
                     GNDSAKQATSRVALSNVRVLAIDQTVADKSPKSDVKTVTLELSAGQVQTLARAQATGT
                     LSLALRALSDQGAKSASLPADSGDDADGGQVSIIRYGIVRNQSGNGGR"
     gene            complement(11214..11726)
                     /locus_tag="VGC27_12650"
     CDS             complement(11214..11726)
                     /locus_tag="VGC27_12650"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY0283460.1"
                     /translation="MAAELLVIVVLPILLALAAGWDLASYTIPNRLQLALVAAFVVFV
                     PVSGMGFTLLGWHLLAGFLGLAAGFALFALGFIGGGDVKLFAAIALWLGFDLDLMNYM
                     LIAALLGGALTVALLLLRRVPLPARLARQPWLLRLQDDHAGVPYGVALAAGAFLILPQ
                     TEVFRLAAAG"
     gene            complement(11801..11965)
                     /locus_tag="VGC27_12655"
     CDS             complement(11801..11965)
                     /locus_tag="VGC27_12655"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0283461.1"
                     /translation="MNNLFARFVRDESGATAIEYGLIAALIAVVIITVLTTIGSNMNT
                     KLNSVASSLG"
     gene            12301..12759
                     /locus_tag="VGC27_12660"
     CDS             12301..12759
                     /locus_tag="VGC27_12660"
                     /inference="COORDINATES: protein motif:HMM:NF025014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEY0283462.1"
                     /translation="MRRTTALLATLLLTVGAAQARSASVSVPLDEVRMLTFAQPISTL
                     YIGNPAVADVTMIDKRHAFVLGKSYGATNIVALDASGAEISNQQVVVSGSTGGAVVTL
                     QRGAARTTYSCAARCEPSPKPGDGKDFDGSMDQIAKHQQFVAKFASDQAQ"
     gene            complement(12823..14346)
                     /locus_tag="VGC27_12665"
     CDS             complement(12823..14346)
                     /locus_tag="VGC27_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY0283463.1"
                     /translation="MSAGKILRELKGRMLKRVCAFVSARRGNVAMIFALALIPLCLAA
                     GAGLDYARAVVVRSNMAEAIDAAALAVGSSPGLTTAQMRTLAQQYFNANYKADPSFGT
                     PAAIVVTPGTQQITVSVTSQMPTTLLSVIGINALTVSASNKVVWGQMKLWVALVLDNT
                     SSMTESDYTGTSKMTALKTASQSLLTMLKNASSIAGDVQVAVIPFSRDVNIGTSYSNA
                     AWLGWTDFNAGPGAPSTSVGPGSACPWQDKNQGYHCQASPTNGSASVTTIPNSGTYKG
                     YICPSANTVGHYYNGCYDSVQSGSKYTHTWKPNPKSSWSGCITDRDRNYDVQNTTPSA
                     GTPATLFVAENSPYCPGATILPLGYDWTKLNNKINSMVANGSTNQTIGLAWGLQALTQ
                     GAPLSPPALPENTQRVIILLSDGLNTQNRWDSSSDFIRANPQVDARMALACANAKAAG
                     ITVYTVFVDLNGTQGNSAVLQNCASDAGKYFDLTTSGAIISTFNQIGQQITNLRVAQ"
BASE COUNT         2659 a         4754 c         4678 g         2483 t
ORIGIN      
        1 atagctccgg agccgcttta attccttctc cagcatctcc ttcacccgga gccgcgaggt
       61 gcgggccgcc tccagcatct ggcttgtatt gagaagcagc gaagcgccct gatgccctct
      121 aggtccggga attcccaact gcaagggcac atcctcacgc accccgacgg caacgaactc
      181 gataaaccat gtctgccgca ccgtcttgag tggattgcct atcaacacga ggctcaggcc
      241 ggtttggcca tcgatctgct ccaactccac aaacacgccg gatctgtccg gccgccagct
      301 atcatcgagg atcggcaact gccgccagcc gcaataaaag tcgcggcaca aggccgggcg
      361 tgtttcgtag atggtacagc cccgggcgca atggcggcag gtcaaacctg cctccttctg
      421 aatttccggc ttgtcgatcg ccatcacggt gcagcagacg gtgcattcgc cgcacgctcg
      481 ccccgcaacc agatcgatgt cagtggccgc catggcgcgg acgtcagcgg ggcaaggcca
      541 tcagaccggt ccagccggtc cagatgattt ggatgccgat gcagaccagg acgaacgccg
      601 acagccgcac cacgacattc gtgccgcccg gacccaggcg cgagatggtg ccttcggcaa
      661 aacgataaca cagataaatc gtcgccgaga tcgcgagcag gccggcaatg gcggcaagcc
      721 caagcaaaac caattccggc aggccggcca ggctcgggcg ctggctgccc agcgcaatcg
      781 ccgtggcgat ggagccggga ccgaccgtca gcggcatggt gaaaggataa aacgtgtcga
      841 gcggtttcgg cgccgggccc gccgccgccg cgtcgccgtc cacgctggcg ccggaattca
      901 gcaaatgcca gccgaacgcg gtcaccacaa ggccgccccc gatccgcacc accggcaggc
      961 tgatgccgaa gaattccagg acatgcgatc ccaccagcag cgaggccagc aacaggaaga
     1021 agctgttgat cgccacccgc ccggccagcg cattgcgctc ccgatgggtg cgggaccgcg
     1081 tcaagccgag aaacagcggg atgttgccga ccgggttgac gatgggaaac agggccgcat
     1141 aacaaagcag gaacgtattc gcaaaagcat tcatcgaaaa atccaaggct tggtcccacg
     1201 cttcagcgta tcagcaaagc gggcactttt cctgaaaacg ctaatggctc ggcgggggaa
     1261 ccgccagctt acccatgtcg cggtagaagc gctcggcgcc cggatgcagc ggcacggtca
     1321 agccgtcggt tgcggtatcg agacggatat aacgggccga aggtgcgccc ttgtcgaaca
     1381 aggcgcggtt ggccggatga tacagcgcgc gcaagatgcc atacacgaca tccggcgacg
     1441 ccgaatcctt caccacccat agagtgcggc tggagacggt ctggatcgcc cccgtgcccc
     1501 gataggcgcc cgccggaatc acgccggcgg aaagactggg cacgcgcagg agaacgcggt
     1561 cgcgcgcctt gccgtcaatc gcaatcaact tggccgtacc gcgcgctatg agatcgccga
     1621 ccagcggcga cggcgccccg cccaggaaga agaaggcgtc gagcttgccc tgctgcagca
     1681 gaccggcggc gacatcgtag gattcgttgc gcagcttcag gcgcttcatc ggaacccggt
     1741 aggcggcaag gatttcgcgc gccaccacgg ccgcgcccga accgtcagca ccgagcgaaa
     1801 cgcgcttgcc gctgagatcg gcgaccttgg cgataggcga cttggcggcc acgaccaact
     1861 gaacggtctc gggaaacaga ttggcgatga cgcggatatg gctctggcgg cccgctttgc
     1921 ggaacgcccc ccgtccggcc accgcgtcgg ccacgacgtt ggcctgcgcc agaccggaat
     1981 cgacgccgcc cgcattgacc gcgagcacat tgccgaccgc cccttcgctg gtgcgcgccg
     2041 agacgatcag gcccggcggc ccgcataccc cgcgcgcccg gcaacgcgcc agacccggcg
     2101 gatggctgac gatgccggcg atcagctcgc ccatcggaaa ataggtcccg gccgtcgagc
     2161 cggtcgccac ctggaaggac agacgcgggg gctgggcctg gccggcggcc gcaaaagccg
     2221 cagccaagag gcccacgaga acaatccaga tcgtccggcg gcgtctcatg ggacggcaat
     2281 cccatatcat tgcgtcagga attggacgcc aaggcttaat aataggtcaa gtttaacagt
     2341 ttgttgaccg cgttcgcgca tgctgtgcct tggaccagtt cgtctagcaa agggttttgc
     2401 catgcaccgt ctgctcgttc gcctgggcgc gctcgccctg tcgaccgccg cgcttgccgc
     2461 atgcgcgtcc gaccccgcca ttccgagcct gaagcaagcc aaaaatgacg ccgcagccgg
     2521 tacgactcca agcctggaag gcgacatccg cacggctcag gtgcagcgcg ccagcggcga
     2581 ctatacgggc gcgatacgct cgctcagtca attgatgctg gtggccccgg acgatccgcg
     2641 cgtcgtcggc gaatacggca aggtgctggt ccagcaaggg cgcgggccgg aagccctaaa
     2701 cttcctcaag cgcgccatcg agctgcagcc aggcgactgg acgctttatt ccgctctcgg
     2761 cgtcacctac gaccagatca aagatcccgt caaagcgcgc gacgcctatc agcgcgccct
     2821 cgtcctcaag ccgggcgaag ccgcggtcct caacaatttc gccctctcgc gcatgctggc
     2881 gggcgatccg gcccaagcca agacgctgat ggcccaggcc gccgcttccg gttccaacga
     2941 gcccaagatc gcgcacaatc tggcgctgat cgacgggctg gccggcccga aggcgccgcc
     3001 cgcggtgcag agcgatgccc ccgcggcgac gccgcaagca accaccgcgc ctgccgccga
     3061 gcacccgccc acgccgctga gtatggccaa gagcgacggc acggccggcc cgaccaccac
     3121 cttcatgcag caagtgccct acgatccgca ggccggaccc gttggcaagc cggcaaaggc
     3181 agcgccgcgc aagctcgcca aagcgccgct caaaacgccg gccgaaacgg tcaagagcac
     3241 gattccggcg ctcaggctgg cgaacgacaa tccgtaattc acggatcaga gcacgatccg
     3301 cttaaatggg atcgcgccgc ctgcatgctt cgacaagctc agcatgaggg gttgtcttcg
     3361 gccttcatcc tgagcctttc gaagggtgac aggaagcgat tcaatcagat tggatcgtgc
     3421 tccagaatac cgggatcgga gaacggagca tttccgttca catcatgtgc atgaccttca
     3481 tcaccgccgg tcccatgatg acgacgaaca ggcacggcag gaagaacagg atcatcggca
     3541 ccgtcagctt ggcgggcaag gcggccgctt tttgctcggc cgcggccatg cgcatctcgc
     3601 ggttctcctg ggcgcagatg cgcagcgcct gccccatcgg cgtaccgtat ttttcggcct
     3661 gattgagggc ggtcgccacc tgcttgacgc cgggatggcc gcagcgcttg gcgagattct
     3721 cgaacgcctg tttgcggtcc ggcagatagg acagttcggc cgtggtcagc gcgaattcct
     3781 ccgccagctc ctcggaggcc gaaccgattt cggaggccac cttggcgaac gccgcttcca
     3841 ccgacatgcc cgattccacg cagatcaaga gcagatcgag cgcgtcggga aaggcgttca
     3901 tgatcgaggt ctgccggcgt gtggtcaggt tggtgacgaa cagatcgggc agatagaagc
     3961 cgaacagggc ggcggccacc gcgatggcca tcttcaaggt tagcgaccag gcgaagtgcg
     4021 tcaccacgaa gagatagaac agcgccagcg cgaacagaat gaagggcatg acgaagcgga
     4081 aaaacatgaa gacgacatgg tgcgtctgac tgcgcaggcc cgccgtcgcc agcttgtcgc
     4141 gcgagtcttc cgactccacc agcttgccga gcttgaagcg ctccacgatc tgcttgatca
     4201 gcttggtggt gccgaccggc tcgctgcgca gcgcgccgcg cctggcgttg agctgggcat
     4261 gatggcgggc gcgcagttcc tcgcgccgtt cggcgaccgc cttcagacgc cggcccagcg
     4321 tgtcgcgctc caacagcggc agacccaggg tgacgatcgt ggcgaaagcc aatattccca
     4381 ccgccgccgt cgccaggaac ttcggatcga agagcagatt cacgatctcg ctattgaaca
     4441 tccgctccgc ctcaatgctt gaagttgatc attttcttca tcacgaagat gcccagcccc
     4501 atccacagga cgcagcccgc cagcagcagg ttgcccaccc gctcggtgaa caacagcgtc
     4561 atgtaggcgg gggaggtgaa atagacgagc gccatgacgc ccggcggcag cgagccgatg
     4621 atcatggccg acgccttggc ttccgacgac atcgccttga tcttgccttg caggcgtttg
     4681 cggtcgcgca gcacggcggc gaggttgccc agcgcctcgc tgaggttgcc gcccgatttc
     4741 tgctgaatcg tcatcacgat ggcaaaaaaa ctgacttccg tggtcggcat gttctcgtac
     4801 atgcgcctga gaccctgctc cagcgtgacg ccgaccttca tgctgtcgac caggcgcgtg
     4861 aactcgctgc cgaccggatc gcggaactcg ccggccacga tcctgagcgc gtcgccggtg
     4921 ggcaggccgg atttcacgct gcgcacgatc acgtcgatgg cgccggcgaa atcgttggtg
     4981 aagcgcttct cgcggcgttt cctgagaaag ttgagcaccc agcgcggcag gccgaagccg
     5041 gtgccgaagg acgccagtgc ggtgatcagc agcgactgct gggtgtagag gcacaaagcg
     5101 gcgacgacga aagccagcag cccggaagcg atccagaagt tgcgcggcgg aacgtcggta
     5161 aagcccgccc ggtcgagacg gcggcgcagc gtcatcgtcg gctttttttc ggcctgcttc
     5221 ttctcgatct ccttgagcag ctgctgcatg cccttgcgcc gctgaccctg cgattccgcc
     5281 gccccgcgcg ccgcgcgcgg ctgcgccttg gcaatgacgg cgatacgttt ttgcgaggtc
     5341 tcgttcgacc cgaaagcgac ggcgaaagcc gctccgccga acgccagaac cagcaaggcc
     5401 acagcaagca tcaccatctc gcctactcct gcagcttgtc gagggcttcc gccagttcgc
     5461 gttccaggcc gtaatagcgc acccgttccc agaaggccgg acgcacgatg ccggtgccga
     5521 tgtggcggcc cttgatccgc ccgccttcgt cctcgcccga catttcgtag gtcagaagat
     5581 cctgggtgat gacgacgtcg ccttccgtgc ccaccacctc ggtgatcttg gtgatgcgcc
     5641 tggtgccgtc gcgcaggcgt tcgccctgaa tgatgacgtc gatcgagccg acgatcatct
     5701 cgcgcacggt cctggtggga agctggaagc cgcccatggt gatcatggat tccagacggt
     5761 tcatggcttc gcgcggggaa ttggcgtgca gcgtgcccat cgagccgtca tggccggtgt
     5821 tcatcgcctg caacagatcg aaggcttccg gtccgcgcac ttcgccgacg atgatgcgtt
     5881 cggggcgcat gcgcaggcag ttgcgcacca gatcgcgcat ggtgatctgg ccctggcctt
     5941 ccagattggg cggccgggtt tccagccgca ccacatgggg ctgctgcaat tgcagttcgg
     6001 cggcgtcttc gcaggtgatg atgcgctctt ccacgtcgat ataggcggtc atgcagttga
     6061 gcagcgtggt cttgcccgaa ccggtgccgc ccgagatcag cacattgcag cggcagcggc
     6121 cgatgatgcc gagcagcttg gcccccgcct cgttgatcga gccgaagcgg atgagatcgt
     6181 ccatccgcag cttgtccttc ttgaacttgc ggatggtgag ggtgggcccg tcgagcgaca
     6241 agggcggcgc gatgacattg acgcggctgc cgtcgagcag gcgcgcgtcg cagatcggcg
     6301 aggattcatc cacccgccgg ccgacctggc tgacgatgcg ctggcagata ttcatcaact
     6361 gggcgttgtc gcggaagcgg atcggcgtca attgcacctt gccggcgact tcgatgaaga
     6421 cgcgcccggc gccgttgacc atgatgtcgg cgatgtcgtc gcgcgccagc agcggttcga
     6481 gcgggccgta gccgagcacg tcgttgcaga tgtcctgcaa caaatgctcc tgctcggcga
     6541 tcgacatcac caccgccttg atcgagatga tctcgttgac gatgtcgcgg atttcctcgc
     6601 gcgccgaatc cgcgtcgagc tgagccaatt gcgccagatc gatggtgtcg atcaaggcgc
     6661 tgaagatcgt ggtcttgacc tgatagtaat cgtcggagcg ctcgtcggcg acgaacgtat
     6721 gctgcggcgg ggcggcaata gcgggcggcg gcgcggcggc gagctcgcgc gcccccgtcc
     6781 cctcggcttt gggcggcggc cgcgcggctt tcgcctccgc gcgcggcggg ggggccaagc
     6841 tgtcagtcgc ggtgcgcttt ccgaacatcg cctaagctct cttctttgcc ttcaacagcg
     6901 agaagaggga ggcgggggat ttctcatttg cctgcttgcg gccggtgacc agtccggcga
     6961 gccggcgcac ggcctccacc gcgggcgacc tttgccccag ttcggccagc atttgtccgt
     7021 tgttcgccgc ctgcccgaac aaggcggcgt cgaacggcag cacgatggac ggttccaagc
     7081 cgaaggtttc cgcgaaatcc ttcgccggaa tctcgggccg cttgggctgg cccacctggt
     7141 tcagcaccag cctgggcgcg gcgtcgttgg gccggttgcg gcgcagggtt tcgaccagat
     7201 tcttggcgtt gcgcagcgag gccagatccg gcgtcgccac caccacgatg tcgtccgccc
     7261 cgatcagggt cgccttgacc cagggttccc aggaatgggg cagatcgacg agcacacaag
     7321 gcgtcgtctg gcggaccgcg tcgatcacgg attcgtaggc gtcgagcccg gcgtcgtatt
     7381 cgcgttccag tatcgcgggc gcggcaaaca gcgaaagata atcgccgcgc cggatcagca
     7441 ggcgttcgag cagaacctcg tcgagccgct cgggcgccgt cagcgcgtcg gcgatgctct
     7501 gccccgcctc gtcgttgaaa tcgagaccga gggtgccgta ggccagatcg aaatcgagga
     7561 tcgtggtgtt gatgtgctgt tcttcggcga tgcaccagcc gacattatgg cagagcgtgg
     7621 acgagcccgc gccgccgcgc gcgccgacga aagcgatcac ccggccgatc gggtgccctt
     7681 ccggatcgct gtaaagaccg gcgatgactt cgatcagatg cagcggatcg accggcgcca
     7741 ccagatattc ggaggcgccg cggcgcatca gttcgcgata aagctcgacg tcgttgaccc
     7801 ggccgatcac caccaccttg gtcgccgcat cgcagacctc ggcgaggctg tcgagttcgc
     7861 ccaaagcctg ctctcccgat agcctggtct ccacgatcag cagattgggc gtcacctggc
     7921 cggcataatg ttcgatcgcc gcggcgatgc cgccgagctg gacgctcaaa ttggccttgg
     7981 ccaggcggcg gtcggcgccg gcccgctgca tcgccgcgcc ggtgtccgga aattcgcaaa
     8041 acgcatggat ggaaatgcgc ggcaccggac gttcgtgcgc ctccgccccg aaggcgggcc
     8101 ggttctcttc atgccgcatc ctggccatct cgcgcgcgcc ccctactggc cgaccacgcc
     8161 ggacactttg cccgactggt cttgactctt atcggcggcc gtcacttcgc ccttttcgta
     8221 tttgccgacc acgatgccgc ggcgcaccgc atcgctctgc gccatcggcc gcggctgcac
     8281 cagatcgcgc ggatcggcga ccatcgcggc gatattgtgc tgcgtggcgc agccgaaatt
     8341 gggcgacgat tgattggccg ccgtgtcgga aagatccttc gaccagtcgc cgcacggatc
     8401 ggtgcgggcg acgtaaccga caaagccgat ctcgacgcgg ctgtcgccgt tctccgcctg
     8461 atgcgttccc accatgatgc gcgaacggtc cacgcccagc gaggccaggc gctccccgaa
     8521 atagccgatc gcggcggagg cgtcgcggcc ggccggcgcc gagatcgaca gcgcgccgtt
     8581 gccgcgttca agatagcggg cgacgaattc gtcgaggcgc gccgcgtcgt cgggcaacag
     8641 gcccgcggcg ggcgccgaga agggcagctt gatcgaattg taatgcgctt ccaccgtgat
     8701 cggatggttc gcggcgccgt cgttggaaag cgtgtactgg ttcagcggcg aggcgcaact
     8761 gcccgccaac aacatggcgg cgacggaggc gtaacgcaga aggttcttca ttttcgcttc
     8821 ctattgcacg atgaatccgg tcaggccctg ggcctgggac accggacgct tgccgcgcga
     8881 atacgtctcg ttcagacggc cgagcaaaat ggtttcggga tcggtgggcg gcaggaagcc
     8941 gtcggtcggc gcggccagct tggcctccgt cgtcggactg acgaggtagg ccgtcaccat
     9001 caccaccagt tcggtttcgt tgttctggaa atcgcggctg cgaaacagcg cgcccaggat
     9061 cggcatgtcc ttgaggccgg gaaatccgtc aagctgctgc ctggtggtgt gctgcatcag
     9121 gccggcgacg gcaaagctgc cgcccgaggg cagttcgatg gtggtctccg tccggcgcac
     9181 cgacagcgcg ggaatggtgg tggcccccac cgtgaccggg ttgccgttgg gatccacgga
     9241 ggtgccgcct tgcagcgtaa aggcgccggt gttggtgagt tcgctcacct cggtcgagag
     9301 ttgcagcgag atgcggccgg aggaaagcac caccggcgtg aaggaaagcc cgacgccgaa
     9361 ttgcttgaag gtgatcgaga cgttgccctg ggtgtcgcgg cccgacggca ccgggaattc
     9421 gccccccgcc aggaatttcg cggtttcgcc ggagacggcg gtgaggttcg gctcggccag
     9481 ggtatgcacc aggcccaccg tttccagcgc cttcatcgcg ccctgcacat tgttgggacc
     9541 gccgaggcag gtgcccgcag ccgtcggcgt gcaggtcatg ccgatcctgg cgccggacag
     9601 atcgctcaac gcctggccca tcagcccgta gggattggag gtcgaggcta tcagcggaac
     9661 gccgttgatc acggcggcgc cggacagatc gatgccgagc tgcttggcga cctggcgctg
     9721 cacttcggcg atgcgcacct tcagcatcac ctgctcgctg ccggaaatct gcagcatgtt
     9781 gacgaccttg gccggatcgc cggcgaagcg cgcggcgatg tcttgcgcgc gggtggacgc
     9841 ctgggcgctg gcgaccttgc cggtgagcac gatattgtcg ttcatcgccg acgccttcac
     9901 gcccgaaccg ggaagctcgg tgcgcatgag gcggttgaga tcggtgacgt ctttctcgac
     9961 gcggatgtcg agcgacagaa gctggcggcc ggtcgcgtcg aagaaaaagg cgttggtctg
    10021 cccgttcttg atcgccaaga gaaaaatccg ccgcggcgtg cgcaccacgg catcgacgat
    10081 ctcgggattg gaaaccagca cgtcgcgcgc gtcggcatcg atctggacga tcgccgcctt
    10141 gtcctgcggc agcaccaggc gctgatggac cggtccgttc gaacgggtgg cgaccgtgat
    10201 cacccgcgcc gcgccgtcct cgtccgcccg cggggcggca aacacgggat ccgccgcgac
    10261 cagaatcagc gcggcggcga tgccgaagac cgggaggaga tatttgtgca tctatctgcc
    10321 cccgttgccg gactggttgc gcacaatgcc gtagcgaatg atcgaaacct gtccgccgtc
    10381 ggcatcgtcg ccggagtcgg ccggcagcga cgcgctcttg gcgccttgat cgctcagagc
    10441 gcgcagcgcc agcgacaaag tgccggtcgc ttgcgcgcgc gccagcgtct gcacctgacc
    10501 ggcggacagt tccagcgtca ccgtcttgac gtcggacttg ggcgacttgt cggcgacggt
    10561 ctggtcgatc gccagcacgc gcacgttgga gagcgcgacg cgcgaggtcg cctgcttggc
    10621 gctgtcgttg ccggtttcgg tgaggatgat atcgacgtga ttgttcggca ggatgaagcc
    10681 gccggcgacg gatgcaatcg acacaggaat ggaaacggcg cgcatccccg gttgcagcgt
    10741 cgccgccatg aagccgctcg agtcgctcct gacgatcttg gcataggtga ccggctcgcc
    10801 cgccaccatc ggcgcgcgcg ccaccgtacc ggcgaccgca gcgtccgccg acagcgcgcc
    10861 cgtctgctga atgaatccgg gatcgacgct gctcgtcggc catttctgcc agcgcaccaa
    10921 tcccgccgag agcggctgcc cgggctgcaa gttgctcgcc gcaacgagca cgttgctgag
    10981 tgctatcggc gcgggctgga gacgcgcatc ggctttcgtc gtgccgccgc cgatcaatcc
    11041 gcgcaccagc aaggctgcgc cgcccgcggc cacggcggcc aacagaatga caacgagacg
    11101 ccgcgtgttc atgggtattg gctcctttgt gccaatccga gcacggaacc ctttagaaaa
    11161 tgcttaatga aatactgaaa ggtcgcgttt gacgggtatc gcccgcgatg ccgtcaaccc
    11221 gccgctgcaa ggcggaagac ttccgtctgc ggaagaatga ggaaggcgcc cgccgccagc
    11281 gccacgccgt aggggacacc cgcatggtcg tcttgcagcc ggagcagcca gggctgcctc
    11341 gccagccgcg cgggcagcgg cactcgccgc agcaacagca gcgctacggt cagcgcgcct
    11401 cccagtagtg cggcgatcaa catgtagttc atcaggtcga gatcgaagcc aagccagagc
    11461 gcaatcgccg caaacagctt gacgtcgccg ccaccgatga agccgagcgc aaacaaggca
    11521 aaacccgcgg ccagccccag gaatccggcc agcagatgcc agcccagcag ggtgaagccc
    11581 atgccggaca ccggcacgaa aacgacaaac gccgcgacca gcgccaattg cagccggttc
    11641 ggaatcgtat agctcgccaa atcccagccc gcggccagcg ccagcaagat cggcagcaca
    11701 acgatgacca gaagctcagc cgccatcctt caccgcttgg taaatgcgag cggccgtcag
    11761 ccgacggccg ctcgcttctt ttgagtccat gcgcgagcct ttatcccagg ctactcgcga
    11821 ccgagttaag cttcgtgttc atattcgagc cgatagtcgt cagcacggtg atgatcacaa
    11881 ccgcgataag cgcggcaatc aaaccgtatt cgatggccgt ggcgccggat tcgtcgcgga
    11941 cgaatcgcgc aaaaaggtta ttcataatga cctccattgt ggacatccca agacgtccgg
    12001 cgcatgccct ttaaccaagc tctagaccgg accaggcaag cttaggtggt ggcgcttaat
    12061 gctgtgttaa gcggatttcg cgctgcgccc gcggattgtg ccgccgcgat acggaaaggc
    12121 ggccgggcga ggcacgccgt cgttaatttt caccatcggt ttaccatggc gcaagatcgc
    12181 gcggcggcgc gccctcgatc ccgttttgca acgcgccgcc gccgggccgg ggaaatacct
    12241 taaaacttct tttacggcgc agcgcctatt ctgcggcgga acaacccgac ggagccgccc
    12301 atgcgccgca cgaccgcttt gcttgcgacc ctattgctga cggtcggcgc cgcccaggcg
    12361 cgcagcgctt cggtgtcggt cccgctcgac gaagtgcgca tgctcacctt cgcccaaccc
    12421 atctccaccc tctatatcgg aaatccggcg gtcgccgacg tcaccatgat cgacaagcgc
    12481 cacgccttcg tcctgggaaa atcctacggc gccaccaaca tcgtcgcgct cgacgcctcg
    12541 ggcgccgaga tcagcaatca gcaggtggtg gtctccggct cgaccggcgg cgccgtcgtc
    12601 accctgcagc ggggtgccgc gcgcacgacc tattcctgcg ccgcgcgctg cgaaccctcg
    12661 cccaagcccg gcgacggcaa ggacttcgac ggcagcatgg atcagatcgc caagcatcag
    12721 caattcgtcg ccaagttcgc ctccgaccag gcgcagtaac gctgcgcacc gtctcgtgca
    12781 tcaagggata gagcgcctgc atgcctaaac gatcgggcgc gtctactgcg ccacgcgcag
    12841 attggtgatc tgctggccga tctggttgaa ggtcgagatg atcgcgccgg aggtcgtcag
    12901 gtcgaaatat ttgccggcgt ccgaggcgca gttctgcagc accgccgagt ttccttgcgt
    12961 gccgttgaga tcgacgaaga cggtatagac cgtgatgccc gcggccttgg cgttggcgca
    13021 ggccagcgcc atgcgggcat cgacctgcgg gttggcgcgg atgaaatcgc tgctgctgtc
    13081 ccagcggttc tgcgtgttca agccgtcgct gagcaggatg atgacccgtt gcgtgttctc
    13141 aggcagcgcc ggcgggctga gcggggcgcc ttgcgtcagc gcctgcaagc cccaggccag
    13201 accgattgtc tggttggtcg agccgttggc gaccatcgag ttgattttgt tgttcaactt
    13261 ggtccagtcg tagccgagcg gcaggatcgt cgcgcccgga caatagggag agttctcggc
    13321 gacaaacagg gtggcgggcg ttcccgccga gggggtcgtg ttctgcacat cgtagttccg
    13381 gtcgcgatcg gtgatgcagc cgctccagct gctcttggga ttgggcttcc aggtgtgcgt
    13441 gtacttgctg cccgactgaa cgctgtcgta gcagccattg tagtaatggc cgacggtgtt
    13501 ggcgctcggg cagatgtagc ccttgtaggt tccgctgttc ggaattgtcg tgacgctcgc
    13561 gctgccgttg gtgggcgagg cctggcagtg atagccctga tttttgtctt gccacgggca
    13621 cgcggagccg ggtcccacgc tggtcgaggg cgcgccaggg ccggcgttga agtccgtcca
    13681 gccgagccag gccgcgttcg aataggatgt gccgatgttc acgtcgcggg aaaacgggat
    13741 caccgcgact tggacgtcgc ccgctatgct cgaggcgttc ttcagcatgg tcagcagcga
    13801 ctgcgaagcg gttttcagcg cggtcatctt gctggtcccg gtataatcgg actcggtcat
    13861 cgacgaggtg ttgtcgagca ccagcgccac ccacagcttc atctggcccc aaaccacctt
    13921 gttggaggcc gagaccgtga gcgcgttgat gccgatcacg ctgagcagcg tcgtcggcat
    13981 ctgactggtg acggagaccg tgatctgttg cgtgccggga gtgacgacga tcgcggccgg
    14041 cgtgccgaag gacggatccg ccttgtagtt cgcgttgaag tattgctgcg ccagggtccg
    14101 catctgcgcc gtcgtcaggc ccggcgagct gcccaccgcc aaagccgccg cgtcgatcgc
    14161 ctcggccatg ttggagcgca ccaccacggc gcgcgcataa tcgagaccgg cgccggccgc
    14221 cagacaaagc ggaatcagcg ccagcgcgaa aatcatggcg acgttgccgc ggcgggcgga
    14281 cacaaaggcg cacacgcgct tcaacatgcg acccttcagt tcgcgcagta tctttcccgc
    14341 agacatggtc cgtgcctccg acacgtgtgc catcatcgcc cgcaatgcgt gaagatggcg
    14401 ttgcttccgc cggttatggt tagagattcg taaacggcgc gccgccgggc cggacaggtt
    14461 aaggattcct tcagcatgac ggcggctttt cctccgccag cgaccgatct caaccgaata
    14521 acaagagaca agcgttaaaa agcggcgaaa ggtaccgaaa ccgtgaacgc tgtt
//
LOCUS       DASWPU010000595         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_4964, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000595 DASWPU010000000
VERSION     DASWPU010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4964"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VGC27_12670"
     CDS             <1..533
                     /locus_tag="VGC27_12670"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283464.1"
                     /translation="RAAVGLLAARVPGLVCVLPTVPHVAALVRAGTADWPAPLHIVEG
                     EDEKFAAFDAASAALAASGTVTTELALSRTPMVVGYKVVWLTAALGRLLIRVKFATIV
                     NLLLEREAVPEFIQERCTPQALADAVAPLLTDKDAAARQVADLDEAMRRLGQGAEQPS
                     LRAARAILDFVRWRGE"
BASE COUNT          245 a          487 c          517 g          253 t
ORIGIN      
        1 tccgcgcggc ggtcggtctg cttgccgcgc gcgtacccgg actggtgtgc gtgctgccga
       61 cggtgccgca cgtagcggcg ctggtgcggg cgggcacggc ggattggccc gcgccgctgc
      121 acatcgtcga aggcgaggac gagaaattcg ccgccttcga cgccgccagc gcggcgctgg
      181 cggcctccgg cacggtgacg accgagctgg cgttgtcgcg cacgccgatg gtggtcggct
      241 acaaggtcgt ctggctaacg gccgcgctgg gcaggctgct gatccgcgtc aagttcgcca
      301 ccatcgtcaa tctgttgctg gagcgcgagg ccgttcccga attcattcag gagcgctgca
      361 cgccgcaagc cctcgccgac gccgtggcgc cgttgctgac cgacaaggac gccgccgcgc
      421 gccaggtcgc cgatctcgac gaagccatgc gccggctagg gcagggcgcg gagcaaccct
      481 ccctgcgcgc ggcccgggcg atactcgatt ttgtgcgctg gcgcggtgaa tagagttcca
      541 ttttctgccc ccgctccgcc caccgaagct ttagcgtagg tggggagcgg ggaaagtggc
      601 tcgcgcggcc tcagccgcgc gagacgaagg gggcggacgc attcgacctt cgttttgctt
      661 ccccattagg ggatgtaaaa aaccttatcc ctcttgcgca ctcccgaatt ttccaaccgc
      721 atcacacgct tgttggaaat gcgccgaact tatccggcac gcaccggagc ggccgtataa
      781 tgctcgcgtt gcttcccatc gagggggcgg ttccggaccg tccgttccgc gggattcaat
      841 cggcgttcgg cgaagcgcgg cgtaacgaac cgcatggagc cggaggtcgc gccattggct
      901 gctgggccgg acaggacctt gacgcaaggc gtccggagca gtgcggggcc gccccgaggc
      961 cggaggcgca cggagacgca aggaccgccg ttcgcggttg ggatagagac caagggtccc
     1021 taatccgcgg cgcgacgtaa aaccccgagg ggttcctcct tgccgagtgc gccgaaggag
     1081 gaagagccga tgtcgccggc cgaaaatcgc cgggcgcggg atgccgtgag gcggaccacg
     1141 cgagcttcgg ctgcaaggcc ggggcagata caggttgtgc gcacgccgct tccgccttcg
     1201 ctaaagctac ggcggacaag tcgcggcgtc ccgcgccgcc cctcgactat gagggggcca
     1261 ctcaaccctc cgggtccatg ggaccgcggg agtgttgacg tatgtttgga gccgtccaac
     1321 atgaatcaac tttactcacc atgggcggcc ttgagccgcc cagccagtca ctggatggcc
     1381 gcttcggaga gcggccatgg tgaacacgtt ggttcagttc gatccataat gactctaccg
     1441 cttctcgacc ggcacgtagt cgcgctgctt ggagccggta tagatctgcc gcgggcggcc
     1501 ga
//
LOCUS       DASWPU010000596         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_109656,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000596 DASWPU010000000
VERSION     DASWPU010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109656"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /gene="nuoI"
                     /locus_tag="VGC27_12675"
     CDS             <1..362
                     /gene="nuoI"
                     /locus_tag="VGC27_12675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY0283465.1"
                     /translation="SPRFRGEHALRRYANGEERCIACKLCEAICPAQAITIEAEPRDD
                     GSRRTTRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEYATETREELYYNKDRLLANGD
                     RWEREIAQSLELDAPYR"
     gene            537..1136
                     /locus_tag="VGC27_12680"
     CDS             537..1136
                     /locus_tag="VGC27_12680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005164.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY0283466.1"
                     /translation="MIQAIAFYVFSFFLIAAAFMVVAARNPVHSVLFLILAFFNAAAL
                     FVLLGAEFLAMILVVVYVGAVAVLFLFVVMMLNVDFTELKRGVRQYLLPGLAVGGLLA
                     LELIFVIAMRWDIAPHAVAAATPAGVSNTAALGHILYTDYIYYFQVAGLVLLVAMIGA
                     IVLTLRKRPGVKRQSVWKQVSRTPETAMDVVDVKPGEGA"
     gene            1138..1446
                     /gene="nuoK"
                     /locus_tag="VGC27_12685"
     CDS             1138..1446
                     /gene="nuoK"
                     /locus_tag="VGC27_12685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY0283467.1"
                     /translation="MTIGLGHYLAVAAALFTIGVFGIFLNLKNIIVILMSIELILLAV
                     NINFVAFSSFLGDLVGQVFALFVLTVAAAESAIGLAILVVFYRNRRSIAVEDVNLLKG
                     "
BASE COUNT          246 a          483 c          510 g          316 t
ORIGIN      
        1 tatcgcctcg cttccgcggc gagcatgcgc tgcgccgcta tgccaatggc gaggaacgct
       61 gcatcgcctg caagctgtgc gaggcgatct gcccggcgca ggccatcacc atcgaagccg
      121 agccgcgcga cgacggcagc cgccgcacca cgcgctacga catcgacatg gtgaagtgca
      181 tctattgcgg cttctgccag gaggcctgcc cggtggacgc catcgtcgag gggccgaatt
      241 tcgagtacgc gaccgaaacg cgcgaggagc tttactacaa taaggaccgg ctgctggcga
      301 acggcgaccg ttgggagcgc gagatcgcgc aaagcctgga actggatgca ccgtacagat
      361 gagcgccacc cgctccaccc cccaattcgt catggccggg cttggtcgcg agcgccagcg
      421 tagcgacgcc atccagtccg cgcgcgtcgg cgtgcgaaaa gagtcattag cccgcggacg
      481 cgggcttgct ggatggccgg ctcgggggcc ggccatgacg aagatgagtt agagccatga
      541 tccaagccat cgccttctat gtcttctcct tcttcctgat cgcggccgcg ttcatggtgg
      601 ttgccgcgcg caatccggtg cattcggtgc tgttcctgat tctcgccttc ttcaacgccg
      661 cggcgctgtt cgtcctgctc ggcgccgaat tcctggcgat gatcctggtg gtggtctatg
      721 tcggcgcggt cgcagtgctg ttcctgttcg tggtgatgat gctcaacgtg gatttcaccg
      781 agctgaaacg gggggtgcgg caatatcttc ttccgggtct ggcggtgggc ggattgctgg
      841 cgctggagtt gattttcgtg atcgccatgc gctgggacat cgcgccccat gccgtcgccg
      901 ccgcgacgcc cgcgggcgtg tccaacacgg cggcgctcgg gcacattctt tatacggact
      961 acatctacta cttccaggtc gccgggctgg tgctgctggt ggcgatgatc ggggccatcg
     1021 tgctgacgct acgcaagcgg ccgggcgtaa agcggcaatc ggtctggaag caggtctcgc
     1081 gcacgccgga aacggcgatg gacgtggtcg acgttaagcc gggcgagggg gcataggatg
     1141 accatcggac tgggccatta cctggcggtc gcggcggcgc tgttcaccat cggcgtgttc
     1201 ggcattttcc tgaacctcaa gaacatcatc gtcatcctga tgtcgatcga gctgatcctg
     1261 ctggcggtga acatcaactt cgtcgccttc tcctcgttcc tgggcgatct ggtggggcag
     1321 gtgttcgcgc tgttcgtgct gacggtggcg gccgccgaat ccgccatcgg actggccatc
     1381 ctggtcgtgt tctaccgcaa ccgccgcagc atcgcggtgg aagacgtcaa cctgttgaaa
     1441 ggctgaggcg tgtacacagc gatcgtcttc ctgcctatca tcgcgtcgac cattgccggc
     1501 ctcggcggcc gcttcatcgg cgcgcggatg tgcgagctgt tgacgaccgt cgccc
//
LOCUS       DASWPU010000597         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_67087,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000597 DASWPU010000000
VERSION     DASWPU010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67087"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..822
                     /locus_tag="VGC27_12690"
     CDS             121..822
                     /locus_tag="VGC27_12690"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0283468.1"
                     /translation="MKLVICAGPATTGKTSVLRHVIHRLLARGLKPAFFKLDVQFAEE
                     DEEIAREFAIPARKVYSGELCPDHCNVMVLADVMQWARKADTDVLLVETAGLCLRCSP
                     YVEGALGMIVLEATSGINLPLKVGPMLSLADVAVMTKIDRISQAEREVFRARIQDVAP
                     AVRVREADALRGIGIDPLIGMILETPDAGDNMMLRGNPPVGTCTICVGKKEIGWQSHF
                     GVVRALDNQTFYRGE"
     gene            824..2008
                     /locus_tag="VGC27_12695"
     CDS             824..2008
                     /locus_tag="VGC27_12695"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY0283469.1"
                     /translation="MALVYLDYAATTPLDPRVVAAMQPCWSETFGNPSSLHSAGREAR
                     AAVDLARERVAKLLNARPQEIIFTASGTEADNLALLGVVGALSAGSLHITVSGIEHPA
                     VRATCRYLESRGVAVTYLPVRADGIVDPEALKRALRPETRLVSVMAANNVLGTIQPTA
                     VLAAIAHEHGALFHTDAVQAAGRIPIDVREQDIDLLSLSAHKIHGPKGVGALFVRDSV
                     RLAPLIHGGGQERGLRSATENVAGIVGLGAAADIANAQMAEDAAKSVALRDRIIDSVL
                     SDFPNAYLFGHRYRRLPGHICLGFAGQEGEAMKLLLALDEAGIAVSAGSACSALSRSE
                     PSYVLTALGFDPFRARGSIRITLGRFTTDADVDRLLEVLKREVSKMRPISTRAFTGGG
                     VG"
     gene            2005..2700
                     /locus_tag="VGC27_12700"
     CDS             2005..2700
                     /locus_tag="VGC27_12700"
                     /inference="COORDINATES: protein motif:HMM:NF015987.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY0283470.1"
                     /translation="MTAGLGLGDLPGKNCGACGFRSCAEMAERLGDTPDLIRRCIQLS
                     DDKFGEGSGAALREAQPQPVPEAARSVSEAGGDWHDSLGREFDFYLEHFPEDPGPREI
                     ILPHNPMLTREMEIAVGDVLIGRPLGMSCGCPITHCGIVREVDRRTGVIVWCVTGPLG
                     PRKSGFKDIGYYIAEGYEGLITKTRSSIGIGLRYFFQPRMCMLQWRHSGLVNFMNRSP
                     AGLQVRLEGLWIG"
     gene            complement(2713..>2951)
                     /locus_tag="VGC27_12705"
     CDS             complement(2713..>2951)
                     /locus_tag="VGC27_12705"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0283471.1"
                     /translation="DRDLEATKRFLERCQIFTLAESLGAVESLIEHPAIMTHATIPAD
                     QRRHLGISDSLVRLSVGIEDADDLIADLDQALSA"
BASE COUNT          500 a          877 c         1013 g          561 t
ORIGIN      
        1 gaacgaagcc gaactggcgc tgaccccggc ggtcaatcgc ctcgacgaca tgctttccgg
       61 cttgcgggag aagatccgca gcggcgagcg tgtaacgcac cgcgaactgg agggcttggc
      121 atgaaactcg tcatctgtgc cggaccggcc accaccggca agacgtcggt gctgcgccac
      181 gtgatccacc gtctgctcgc gcgcggcctg aagcccgcat ttttcaagct cgatgtgcag
      241 tttgcggaag aggacgaaga aatcgcgcgc gagttcgcca ttccggcgcg caaggtctat
      301 tcgggcgaac tctgccccga ccattgcaac gtcatggttc tggccgacgt catgcagtgg
      361 gcgcgcaagg ccgataccga tgtgcttctg gtggagacgg ccgggctgtg cctgcgctgc
      421 tcgccctatg tcgaaggcgc gctggggatg atcgtcctcg aggccaccag cggcatcaat
      481 ctgccgctca aggtcgggcc catgctgtcg cttgccgacg tggcggtgat gaccaagatc
      541 gaccgcatct cccaggccga acgcgaagtg ttccgtgccc gcattcagga tgtcgctccg
      601 gccgttcgtg ttcgcgaagc cgatgcgctg cgcggcatcg gcatcgatcc tttgatcggc
      661 atgattctcg aaacgccgga cgcgggggac aatatgatgc ttcgcggcaa tccgccggtg
      721 ggaacctgca cgatctgcgt cggcaagaag gaaatcggct ggcagtctca tttcggcgtc
      781 gtgcgggcgt tggacaacca gacgttctac cgcggcgagt gacatggctc tcgtctatct
      841 cgattacgcc gcaaccacgc cgctcgatcc ccgggttgtc gcggcgatgc agccttgctg
      901 gtcggaaacg ttcggcaatc cttcgagcct gcattcggcc ggccgtgagg ctcgcgccgc
      961 cgtggacttg gcgcgagagc gcgtggcgaa gctgctcaac gcgcggccgc aggaaatcat
     1021 tttcaccgcg agtgggacgg aagccgacaa tctggccttg cttggcgttg tcggagcgct
     1081 ttcggccgga agccttcaca tcacggtcag cgggatcgaa catccggccg tgcgggcgac
     1141 atgccggtat ctcgaaagcc gcggcgtcgc cgtgacctat ctgcccgtga gggctgacgg
     1201 cattgtcgat cccgaggcgc taaagcgggc cctgcggccg gaaaccaggc tcgtttcggt
     1261 gatggccgcg aataacgtcc tcgggacaat ccagccgacc gcggtgctcg cggccatcgc
     1321 gcatgagcac ggcgcgctgt tccatacgga tgccgtgcag gcggcgggca ggattccgat
     1381 cgacgtgcgc gagcaagaca tcgatcttct gtcgctttcc gcgcacaaga ttcacggacc
     1441 gaaaggcgtg ggggccttgt tcgtgcgcga tagcgtgcgt cttgcgccgc tgattcatgg
     1501 cggcggacag gaacgcgggc tgcgttcggc aaccgaaaat gtggccggca tcgtgggatt
     1561 gggtgcggcg gcggacatcg cgaacgcgca aatggcggag gatgccgcga agtccgttgc
     1621 gctgcgcgac cgcatcatcg acagcgtgct gtccgatttt ccgaacgcct atctgttcgg
     1681 gcatcgatac aggcggttgc ccgggcacat ctgtcttggc ttcgccgggc aggagggcga
     1741 ggcgatgaaa ctcctcctgg cgctcgatga agccggtatc gcggtttcgg cgggcagcgc
     1801 ctgcagcgca ctcagccgct ccgagccgtc atatgtgctg acggcgctcg gcttcgatcc
     1861 gttccgggcg aggggatcga tccggatcac gctggggcgc ttcacgacgg atgccgacgt
     1921 ggaccgtttg ctggaggttc tgaaacgcga ggtgtcgaaa atgcggccga tctccacgcg
     1981 ggcgttcact ggaggaggtg tcggatgacg gctggcttgg gcttgggcga tttgccgggc
     2041 aagaactgcg gcgcctgcgg ttttcgctcc tgtgcggaga tggcggagag gctgggcgat
     2101 acgcccgatc tgatccggcg ctgtatccag ctttcggacg acaaatttgg cgaagggtcg
     2161 ggcgcagcct tgcgggaggc acagccgcag ccggttcccg aggccgcgcg gtccgtttcg
     2221 gaagccggcg gcgattggca tgacagcttg gggcgcgagt tcgatttcta tcttgaacat
     2281 tttccggaag accccggccc gcgcgagatc atcctgccgc acaatcccat gcttacgcgc
     2341 gagatggaga tcgcggtcgg agacgtgttg atcggacggc cgcttggaat gtcgtgcggt
     2401 tgcccgatca cgcattgcgg catcgtccgg gaggtggacc ggcgtaccgg cgtcattgtc
     2461 tggtgcgtca ccgggccgtt ggggccgcgc aagagcgggt tcaaggatat cggctattac
     2521 atcgccgaag gatatgaggg cctgatcacg aagacccgct cctcgatcgg catcggattg
     2581 cgctatttct tccagccgcg gatgtgcatg ctgcaatggc gtcacagcgg actggtcaat
     2641 ttcatgaacc gttcgccggc gggacttcaa gtcagacttg aaggcctgtg gatcgggtga
     2701 ccgcggcgga tttcaggccg agagcgcctg gtccaggtcc gcgatcaaat cgtcggcgtc
     2761 ttcgatcccg acggagagac ggacgaggga atcggaaatt ccaagatgcc gcctctggtc
     2821 ggccggaatg gtggcgtgcg tcatgatggc cgggtgttcg atgaggctct ccacggcgcc
     2881 gaggctttcc gccagcgtga aaatctggca gcgctcgagg aagcgcttgg tggcctccag
     2941 gtcgcggtcc a
//
LOCUS       DASWPU010000598         4372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_54001,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000598 DASWPU010000000
VERSION     DASWPU010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54001"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            538..2712
                     /locus_tag="VGC27_12710"
     CDS             538..2712
                     /locus_tag="VGC27_12710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0283472.1"
                     /translation="MTALALLVVALGDVMLHGRLSLLAPGAFVALAGIIIAYVLYSLS
                     VAHERRELADRQAEQIKSVVKRLESSLAAADAINARLNQSEARYKGLVDAQGDAIFRR
                     TPDSRLTYGNDAFFKLFGLNPLTALGKPFAPELHPDSRAPAFGSFAGLETGRARVSYD
                     QQVRTAYGWRWLAWEDYVVRDNIGRPVEVQSVGRDITERKALEDALTEARDKAEAASR
                     AKSGFLATMSHEIRTPMNGVLGMARLLLETDLRAEQRSYVEAIEQSGEALLALIGDIL
                     DFSKIESGMLVLDEEEADVRALIDGVVELLGTRAHTKGIELIAVVGPEVPVAIRADGI
                     RLRQVLTNLVGNAVKFTEKGGVRIGASVTNGRERNYLRFEVRDTGVGVPAEKRKEIFD
                     EFVQADSSHARKFEGTGLGLAICRRLVNAMGGEIGIEAPPGGGSLFWFTLPSVVAVPA
                     AEQDAPLAGMRVAVVSRNSVLREGLGAQIRAAGGDVAGLTEDGIRRSDIMLIDAGTGA
                     EPAPPTGPLPQIPALVLLPPVARSKLSELRSMGFTGYLVKPVRQSSLVERLRARPSPE
                     AMEAGQQAEQAATHAPPPAAETGPVGLKILLAEDNPINALLTRELLRRRGHLVTVVTS
                     GEAAVKAMEKDGFDLLLTDIHMPGMDGIEAAKAIRQIEARLSRPRTPIIALTADALET
                     GRRACREAGMDGFLTKPVEPTQLDDMFAAFFPVESPVRPAAA"
     gene            2709..4115
                     /locus_tag="VGC27_12715"
     CDS             2709..4115
                     /locus_tag="VGC27_12715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0283473.1"
                     /translation="MSTPASGAKPHSSFAAYFQPRTLVMLALGFSSGLPFLLVGNTFG
                     YWLRDEGTSLTAIGFISWVGIAYSLKFLWAPVIDRVSAPFFGRLGRRRSWMALAQITV
                     ALALIAMAATGPKLSLVLLGAFALIVAFASATQDIVVDAWRIESAGSSEELGLLSSAY
                     QFGYRAALLAADALILVSAEQFGWPISYTLFGALMTVGFVSTMVAAEPIQADKVIEEK
                     ARQAPLSTLNGLQDAIAGPFIAFFRTYGVFALLMLTAISLYRLPDFVMGPMANPLYHD
                     IGVSKDWVGAVRASIGLIASFAGIAAGGFWAFRFGYISALVVGGILQALAVAAFACLA
                     HVIPSAELPSLVALSFGDFLHVVLNNPHLRLFALVMTGDNFGISFAGVALVTYMSSLT
                     SLGYTATQYALLASTYAWAGKILKGFSGAIVERLSVTYGLMDAYAIFFVGCGLIGLPA
                     VFLFLLLAARHRSARKSI"
BASE COUNT          716 a         1450 c         1368 g          838 t
ORIGIN      
        1 tcggtggcgg tgatgtcggt caaggcgata ccggccgtgg gtgagggtgc ggtattagtt
       61 cggaattggg caacaataca gaagggcttg ccggaaaatc acaatccgta ccgtccccgc
      121 ggtcaggccc gccggcatca ggacgtgtcc tcgcatacaa accccctgca atataacgcc
      181 ttcggcggat tcttcccgct ccggaagcca cccggtccac cgtgcagaag cgctatcacg
      241 acgtctctgc atgcaacgtc catcacagtt ttatgaagct tttgtgacga acgattccgc
      301 gccggcgaaa tataagttaa tatattgata ttactatata aatacgcttt actatcaatc
      361 cgcgaaggag ttttccgaac ggcgtttgac ggccaatccg aacagaccgg gagcagagcg
      421 cgccgattgt cgccgcgccc ctccgtaaac gattttcaag gggtaggcgg ctaagatttt
      481 cacccgctat cggaggatag ggtcacggcc tcgcgcctca ttcgtattgt tttcggcttg
      541 accgcgcttg cccttctggt ggtggcgctg ggcgacgtga tgctgcatgg gcggctgagc
      601 ctgctggcgc ccggcgcttt cgtggcgctg gccggcatta tcatcgctta cgtcctgtac
      661 agcctgtcgg tcgcccacga gcgccgcgag ctggcggacc ggcaggcgga acagatcaag
      721 agcgtcgtca agcgcctgga atcctcgctc gccgccgccg acgccatcaa cgcccggctc
      781 aatcaaagcg aggcgcgcta caaggggctg gtcgatgccc agggcgacgc cattttccgc
      841 cgcacgcccg acagccgcct cacctacggc aacgatgcgt tcttcaaact cttcggactc
      901 aatccgctta ccgctctggg caagcccttc gcgccggaac ttcatcccga cagccgcgcc
      961 cccgccttcg gcagcttcgc cggcctcgaa accggtcgcg cccgcgtcag ctacgaccag
     1021 caagtgcgca ccgcttatgg ctggcgctgg ctcgcctggg aagactatgt ggtgcgcgac
     1081 aatatcggcc gcccggtcga agtccagagc gtcggacgcg acatcaccga gcgcaaagcc
     1141 ctcgaagacg cactgaccga agcgcgcgac aaggcggagg ccgccagccg cgccaagtcg
     1201 ggctttctgg ccaccatgag ccacgaaatc cgcaccccca tgaacggcgt cctcggcatg
     1261 gcgcgcctgc tgctcgaaac cgacctgcgc gccgaacagc gcagctacgt cgaggccatc
     1321 gagcagtccg gcgaggcgct gctggctttg atcggcgaca tcctggattt ttccaagatc
     1381 gagtccggca tgctggtgct ggatgaagag gaggccgatg tccgtgcgct gatcgacggc
     1441 gtcgtcgagc ttctcggcac ccgcgcgcac accaagggca tcgaactgat cgccgtggtc
     1501 gggcccgaag tcccggtggc gatccgcgcc gacggcatcc ggctcagaca ggtgctcacc
     1561 aacctggtcg gcaatgccgt caaattcacc gagaagggcg gggtgcgcat cggcgcctcc
     1621 gtcaccaacg gccgcgagcg caattatttg cgcttcgaag tgcgcgacac cggcgtcggc
     1681 gtgccggccg aaaagcgcaa ggagattttc gacgaattcg tccaggccga ttccagccac
     1741 gcccgcaaat tcgaaggcac cggcctggga ctggccatct gccggcgcct ggtgaacgcc
     1801 atgggcggag aaatcgggat cgaggcgccg ccgggcggcg gcagtttgtt ctggttcacg
     1861 ctgccctccg tcgtcgcagt gccggcagcg gagcaggacg ccccgctcgc cggcatgcgc
     1921 gtcgccgtgg tcagccgcaa cagcgtgctg cgcgaaggct tgggcgccca gatccgcgcc
     1981 gcgggcggcg atgtcgccgg gctgaccgag gacggcatcc gccgctccga catcatgctg
     2041 atcgacgcgg gaaccggcgc cgaaccggcg ccgccgaccg gcccgctgcc gcaaattccc
     2101 gccctggtcc tgctgccgcc ggtggcccgt tccaagcttt ccgagcttcg ctcgatgggt
     2161 ttcacgggct atctggtgaa gccggtgcgc caatcctcgc tggtcgagcg gctgcgcgcg
     2221 cggcccagcc ccgaagccat ggaggccggc cagcaggcgg agcaggcggc cacgcacgcg
     2281 ccgccgccgg cagcggaaac gggtcccgtg ggactcaaaa ttctgcttgc cgaggacaat
     2341 ccgatcaacg cgctgctgac gcgggaattg ctgcgccggc gcggtcatct ggtcaccgtc
     2401 gtcaccagcg gcgaggcggc ggtgaaggcg atggaaaaag acggtttcga cctgcttctc
     2461 accgacattc acatgccggg gatggatgga atcgaagcgg ccaaggccat tcgccaaatc
     2521 gaagcccggc tatcccgccc gcgcacgccg atcatcgcgc tgaccgccga tgcgctggaa
     2581 accggacgca gggcttgccg tgaggccgga atggatggtt tcctcaccaa gcccgtcgaa
     2641 ccaacccaac tggacgatat gttcgccgcc ttctttcccg tcgaatcgcc tgtacgcccc
     2701 gccgccgcat gagcactccg gcgtccggcg cgaaaccgca ttcctctttc gccgcctatt
     2761 tccagccgcg cacgctggtg atgctggcgc tcggcttttc ctcgggcctg ccgttcctgc
     2821 tggtgggcaa caccttcggc tattggctgc gcgacgaagg cacctcgctg accgcgatcg
     2881 ggttcatctc ctgggtcggc atcgcctatt cgctgaaatt cctctgggcg cccgtgatcg
     2941 accgggtgag cgcgccgttc ttcggccgcc tgggccggcg gcggagctgg atggcgctgg
     3001 cccagattac ggtggcgctc gctttgatcg ccatggcggc gacgggtccc aaactatcgc
     3061 tcgtgctgct gggtgcgttc gcgttgatcg tggcgttcgc ctcggccacg caggatatcg
     3121 tcgtcgatgc ctggcgcatc gagtccgccg ggagcagcga agagcttggt ctgctgtcgt
     3181 ccgcttatca gttcggctat cgcgcggccc tgcttgccgc cgacgccttg atcctggttt
     3241 ccgccgagca gttcggctgg cccatctcct atacgctctt tggcgcgctg atgacggtcg
     3301 gctttgtctc gaccatggtg gcggccgaac ccatccaggc cgacaaagtc atcgaagaga
     3361 aggctcgcca ggcgccgctt tcaaccctga acggcctgca agacgccatc gccggaccgt
     3421 tcatcgcctt ctttcgtacc tacggcgtgt tcgcgctgct gatgctcacc gccatcagcc
     3481 tttaccgcct gccggacttc gtcatggggc cgatggccaa tccgctctat cacgatatcg
     3541 gcgtgtcgaa ggattgggta ggcgccgtgc gcgcctccat cgggctgatc gcttcgttcg
     3601 ccggcatcgc cgcgggcggc ttctgggcgt tccgcttcgg gtatatcagc gcgctggttg
     3661 tcggaggcat cctgcaagcg ctggcggtgg cggccttcgc ctgccttgcc catgtcatcc
     3721 ccagcgcgga gcttccttcc ctcgtcgcgc tgtcgttcgg ggatttcctg cacgtcgtgc
     3781 tgaacaaccc gcatctgcgg cttttcgcgc ttgtcatgac aggcgacaat ttcgggatca
     3841 gtttcgcggg cgtggccctt gtcacctata tgtcgagcct gacgagcctc ggttataccg
     3901 cgacgcaata cgccttgctt gcttcgacct atgcctgggc cggaaaaatc ctcaagggat
     3961 tttcgggcgc cattgtcgaa cgcctgagcg tgacctacgg gctgatggac gcctacgcga
     4021 tcttcttcgt cggctgcggc ttgatcggat tgccggccgt gttcttgttc cttttgttgg
     4081 cggcacggca tcgttccgcg cgcaaatcga tctgaacaag cgcgttagcc acgatggctt
     4141 tgggatttgt tttgtccgcc gccgtggggt aaaatccttc tttgcgcagt ggcagatgcc
     4201 cgaaggttcg caatccgcct tcgcccaagg gaagcgcctc cggtggtgaa tattcacgag
     4261 cctggtcccc ttgagggccg cgtcgagcaa tggcccgttc tggccgcgtt cggcgccatg
     4321 gaagtgcggc tggccgaaac cgacaccgag gtcgaggcgg cgcaacgcct gc
//
LOCUS       DASWPU010000599         4679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_60415,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000599 DASWPU010000000
VERSION     DASWPU010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60415"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..688)
                     /locus_tag="VGC27_12720"
     CDS             complement(68..688)
                     /locus_tag="VGC27_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0283474.1"
                     /translation="MLLWTGLFLIVLGVACIAIDRPATHFIYDRVSGRFHAFLAATTH
                     FAKAAHWLAAACLAYVATWAWLRWIGENPGIRLASQAALAFLISLALGSAVLHGTKLV
                     LGRRRPRDELEMNRYGFIWFGFNLDWNSFPSGHALTIMCVATIAAALWPGLAILWFAA
                     ALWLGLTRALLTAHFLSDVFVGAGVGLISAREVLVNLFPPLAQPWF"
     gene            complement(872..2032)
                     /locus_tag="VGC27_12725"
     CDS             complement(872..2032)
                     /locus_tag="VGC27_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY0283475.1"
                     /translation="MPAENLRSVPEEDCGKAYRAYRLMAEEASDIVILHEPDAGILYA
                     SSALERVLKRTTADIEHHRFMDLVHPDDIDAAASIHAIPLPGQMLTATYRVLHGDGHY
                     VWIETTTRGIYDQQTGALRNTISVSRDVTERMNRELEVKAAQERAEAANKAKSRFLAN
                     MSHELRTPLNAVIGFTDLMRQRTFGPLGNERYEEYATLIYDSGQLLLDLISDMLDMAK
                     IEAGRLELNYERVDVNGTVEDCVRMLRDRAENNGLELTLSLPREPLFLIADKRAVKQV
                     LLNLLTNAIKFTPAGGHVGVGVRYGDGLATITVRDTGVGIPESELPRLGNPFEQVNSD
                     PMLAKGGSGLGLALVRALMDKHGGSLVIDSEEGVGTTVRITFPLIANKRAAA"
     gene            2280..2762
                     /locus_tag="VGC27_12730"
     CDS             2280..2762
                     /locus_tag="VGC27_12730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0283476.1"
                     /translation="MRPILTAMLTAAALTGCSPPSSGDPLSVEANAAYLADNAKRSGV
                     VSVPGIQYRVVKSGNGAQPGRHDCVTVNYKGSLINGKVFDQTKPGQPATFPAGALIPG
                     WVEALQMMHAGDQWTLVIPAGLAYGKTGAGDGLIPPNQTLVFEIELLKVAPPTQAGCE
                     "
     gene            complement(2683..3516)
                     /locus_tag="VGC27_12735"
     CDS             complement(2683..3516)
                     /locus_tag="VGC27_12735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0283477.1"
                     /translation="MNRAQWNHLADEFETQVCDIAREETEDRLGRYVRAAHPVPKSSV
                     LVDLGCGLGSFIRKFQDRFLKITAVEYAPRIIARAKKNCAGVTKIKWLAMDIPRSAET
                     IGTVADLTVCMNVITSPSEAKRNAIWNAVETVTKPDGYALIVVPSLESERMVQRVSRR
                     GRHAKVPAAMPGGLVQRDDAVQKHFERQEFGTILSRHGFVAVRLGRAYYPWAKEGMRK
                     PRFAGATGPWDWVCLAQRRSDCPKQTSARAAGGPIRIRPASAGPLSRVRSRKPRSGSA
                     E"
     gene            complement(3584..>4679)
                     /gene="purD"
                     /locus_tag="VGC27_12740"
     CDS             complement(3584..>4679)
                     /gene="purD"
                     /locus_tag="VGC27_12740"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY0283478.1"
                     /translation="ENKIGFAVIGPEVPLVAGLADRFEAAGIKALGPSAKGAILEGSK
                     GFVKDLCDDHGIPTARYRRFREPLPAKSFAKTLGLPVVIKADGLAAGKGVIIAETVDD
                     AIRAIDFMFDGAFGAAGTEIVVEEFLDGEEASFFALTDGEHVLALTGAQDHKRAFDGD
                     QGPNTGGMGAYSPAPVLTQAVIDKTMNRIVKPTVAAMAERGRRYMGVLFAGLMIKNGE
                     PSLIEYNCRFGDPETQVLVTRLRSDLATALLAARDGVLDAIDLRWRDDAALTVVMAAK
                     GYPGDYAKGSEIRGLDEAAKQDGVQIFHAGTARRAGKIVAAGGRVLNVTATAETVADA
                     QKRAYAAVDLIDWPQGFCRRDIGWRAIARG"
BASE COUNT          821 a         1471 c         1529 g          858 t
ORIGIN      
        1 acggctcagg atgaggcgcg ggccatgaca ggccggtgag cgcactgtcg gtaattaagt
       61 ccagactcta gaaccagggc tgggcaagcg gcgggaaaag gttgaccagc acctcgcgcg
      121 cgctgatcag gccgacgccg gcgccgacga acacgtcgct caggaagtgc gcggtgagca
      181 gggcccgcgt cagtcccagc cacagcgccg cggcgaacca caggatcgcc agcccgggcc
      241 acagcgccgc ggcgatcgtg gcgacgcaca tgatcgtcag cgcatgcccg gaaggaaacg
      301 agttccagtc gagattgaag ccgaaccaga taaagccgta gcggttcatc tccagttcgt
      361 cgcgcgggcg cctgcggccg agcaccagct tggttccgtg caacacggcg ctgccgagcg
      421 ccaggctgat gaggaaggcg agagcggcct gcgaggccag ccggatgccg ggattctcgc
      481 cgatccagcg cagccaggcc caggtcgcta cataagcgag acaggccgcc gccagccaat
      541 gcgccgcctt ggcgaaatgc gtcgttgccg cgaggaaggc gtgaaagcgt ccgctcaccc
      601 ggtcgtagat gaaatgcgtg gcgggccggt cgatggcgat gcaggccacg ccgagaacga
      661 tcagaaacag tcccgtccaa agcagcatgg cgactgcttg ccgcgacgga aggccgctgt
      721 caaatcgccc ctagagcgca atccggcaaa gtggtcggta gcagcagcgt gccgacgcgg
      781 tttgccgtca gattgcgcga aaatcaagga atcgagagct tgatccgatt caattatatc
      841 ggatcgcgct cgaggccggg cggcaaaggg atcaggcggc cgcgcgcttg ttggcgatca
      901 acgggaaggt gatgcggacg gtggtgccga cgccttcctc gctgtcgatc accagtgagc
      961 cgccgtgctt atccatcagc gcgcgaacca gcgccaggcc gagacccgag ccgcccttgg
     1021 cgagcatcgg atcgctgttg acttgctcga agggattgcc cagccgcggc aattcgctct
     1081 ccgggatgcc gacaccggtg tcgcgcacgg tgatggtcgc cagaccatca ccgtagcgca
     1141 caccgacgcc gacgtgacct ccggccggcg tgaattttat ggcgttggtc agcaggttga
     1201 gcagaacctg cttcaccgcc cgcttgtcgg cgatcaggaa cagcggctcc cggggaagcg
     1261 acagcgtgag ttccagtccg ttgttttcgg cgcggtcgcg caacatgcga acgcagtctt
     1321 cgacggtccc gttgacgtcg acgcgttcgt aattgagttc gagcctgccc gcttcgatct
     1381 tcgccatgtc gagcatgtcg gagatgaggt cgagcagaag ctggccggaa tcgtagatca
     1441 gagttgcgta ttcctcatag cgctcgttgc cgagcgggcc gaaggtgcgc tggcgcatca
     1501 gatcggtgaa gccgatgacg gcgttgagcg gcgtgcgcag ctcgtggctc atgttggcga
     1561 ggaagcgcga cttcgccttg ttggcggcct cggcgcgctc ctgcgcggct ttgacctcca
     1621 gttcgcggtt catgcgctcg gtgacgtcgc gcgagacgct gatcgtgttg cgcagggcgc
     1681 cggtctgctg gtcgtagatg ccgcgcgtcg tggtttcgat ccagacataa tgtccgtcgc
     1741 cgtgaagtac gcgataggtg gccgtcagca tttgccccgg cagcggaatg gcgtggatgc
     1801 tggcggcggc gtcgatgtcg tcgggatgaa ccaggtccat gaacctgtga tgttcgatgt
     1861 ccgcggtcgt ccgcttgaga acccgttcca gtgcggagga ggcatagagt atgcctgcgt
     1921 cgggttcgtg caggatcacg atatccgagg cttcctcggc catgaggcga taagcgcggt
     1981 acgcctttcc gcaatcctct tccggaacgc tccgaaggtt ttcggccggc atgacattcc
     2041 ccgatctacg caaaaaatac tcgcttcatc acaagcctag ctccatcgtg ttaatcaaca
     2101 atttcgcaac tcaaaggcgt gagagcagcg ttaagtttcg cttaatcgca ggcctgcgga
     2161 gcaaggcttc gttcagtctt tcgcgccggg acgggcgcag gatcgtactg gcgtaagcaa
     2221 gaagccctct ataaaggaga aagccgccga acgatttgcg aagcagagga gttttcgaaa
     2281 tgcgtccgat cttgaccgcc atgcttaccg ccgccgcgct gaccggatgc tcgccgccgt
     2341 cgtccggcga tccgttgagc gtcgaggcga atgccgccta tcttgcagac aatgcgaagc
     2401 ggtccggtgt cgtttccgtg cccggcatcc agtatcgggt cgtcaagagc ggaaacggcg
     2461 cgcagcccgg ccgtcacgat tgcgtcaccg tcaattacaa gggctcgctg atcaacggca
     2521 aagtgttcga ccaaaccaag ccgggccagc ccgccacatt tcccgccgga gcgctgatcc
     2581 caggctgggt cgaggcgctg cagatgatgc atgccggcga ccagtggacc ctcgtcattc
     2641 cggcgggctt ggcctacggc aagaccggcg ccggtgatgg gcttattccg ccgaaccaga
     2701 ccttggtttt cgagatcgaa ctcttgaaag tggccccgcc gacgcaggcc ggatgcgaat
     2761 aggcccgccg gccgcgcgtg cgctcgtctg ctttgggcaa tcactcctgc gctgggctag
     2821 gcagacccag tcccaaggcc cggtcgctcc ggcgaagcgg ggtttgcgca tgccttcttt
     2881 ggcccacggg taataggcgc gccccaggcg cacggcgaca aatccgtgcc gcgacaggat
     2941 ggtgccgaat tcctgccgct cgaaatgttt ctggacggcg tcgtctcttt gcaccagccc
     3001 gccgggcatc gcggccggaa ctttggcgtg gcggccgcgg cggctcaccc gttgcaccat
     3061 gcgttcggat tcgagcgacg gtacgacgat cagcgcatag ccgtccggtt tggtgacggt
     3121 ctcgaccgcg ttccagattg cgttccgctt cgcctcgctc ggcgaggtga tgacgttcat
     3181 gcacacggtg agatccgcga ccgtaccgat ggtctcggcg gaacgcggga tgtccatggc
     3241 aagccatttt attttcgtta cgcccgcgca gttcttcttg gcgcgggcga tgatccgcgg
     3301 ggcgtattcc accgcggtga tcttcaggaa gcgatcctgg aatttgcgga tgaagctgcc
     3361 cagcccgcaa cccagatcga ccagaacgga actcttcggg accggatgtg cggcgcggac
     3421 gtagcggccg agccggtctt ccgtttcttc gcgcgcgata tcgcagacct gcgtctcgaa
     3481 ttcgtccgcg agatgattcc actgcgcgcg attcatcgcc gaacaactcc agaatcatgg
     3541 gtgacacgga agtgtaggcg cttttttcgc gcgagatcac gatttatccg cgtgcgatcg
     3601 cgcgccaacc aatgtcgcgc cggcagaatc cttgcggcca gtcgatcaga tcgacggcgg
     3661 cataggcgcg tttctgcgca tcggccacgg tttcagccgt ggcggtgacg ttcagcacgc
     3721 gccctccggc ggccacgatc ttgccggcgc ggcgcgccgt gcccgcatga aaaatctgta
     3781 cgccgtcctg tttcgccgct tcatccaagc cgcggatctc cgagcctttg gcgtagtcgc
     3841 cgggatagcc tttcgccgcc atgaccacgg tgagcgcggc atcgtcgcgc cagcgcaggt
     3901 cgatggcgtc gagcacgccg tcgcgcgcgg cgagcagcgc ggtcgccagg tcggatctga
     3961 gccgggtcac gagcacctgg gtttccgggt cgccgaaacg gcaattatat tcgatcaggc
     4021 tcggctcgcc gttcttgatc atcagccccg cgaacagaac gcccatatag cgcctgccgc
     4081 gctcggccat cgccgcgacg gtcggcttga cgatgcggtt catcgtcttg tcgatgaccg
     4141 cttgcgtcag caccggcgcc gggctgtagg cccccatgcc gcccgtgttc gggccctgat
     4201 cgccgtcgaa ggcgcgcttg tggtcctgcg cgccggtcag cgccagcaca tgctcgccgt
     4261 cggtgagggc gaagaagctc gcttcctcgc cgtcgaggaa ttcttccacc acgatttcgg
     4321 ttccggccgc gccgaaggcg ccgtcgaaca tgaaatcgat ggcgcggatc gcgtcgtcca
     4381 cggtttcggc gatgatgacg cccttgccgg cggccagccc gtccgccttg atgacgaccg
     4441 gcaaccccag cgtcttggca aaggatttgg cgggcagcgg ctcgcggaag cggcggtagc
     4501 gggcggtcgg gatgccgtga tcgtcgcaca gatccttcac gaagcccttg gagccttcca
     4561 ggatcgcgcc cttggcgctg ggtccgagcg ccttgatgcc ggcggcttcg aagcggtcgg
     4621 ccaggccggc gaccagggga acttccggcc cgatcacggc aaagccgatc ttgttctcc
//
LOCUS       DASWPU010000600         4205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_60419,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000600 DASWPU010000000
VERSION     DASWPU010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60419"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /gene="asnS"
                     /locus_tag="VGC27_12745"
     CDS             complement(<1..1249)
                     /gene="asnS"
                     /locus_tag="VGC27_12745"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEY0283479.1"
                     /translation="MDKKEFSVRAILAGEAPKDAPVAVKGWVRTRRDSKAGISFLQVS
                     DGSTFHPVQVVVPNTLPNYTDEVLKLTAGCAVEAVGMIVPSPSKGQPFEMQASAVKVI
                     GWVEDPDTYPIQPKPHSLEFLREVAHLRPRTNVIGAATRVRHTLAQAIHRFFDENGFF
                     WVNTPIITSSDAEGAGEMFRVSTLDFANLPRTPEGKVDFSKDFFGRDAFLTVSGQLNV
                     EAYCLALTKVYTFGPTFRAENSNTSRHLAEFWMVEPEIAFADLSDDATLAERLLKSAF
                     ETLLKERHEDLAFFDERIEKGLVAKLEGIVGSEFVRMDYSEAVAVLERSKEKFEFPVK
                     WGVDLQSEHERYLTEKHARKPVIVMNYPKDIKAFYMRVNDDGKTVAAMDVLAPGIGEI
                     IGGSQREERLDVLDARMTAMGIDT"
     gene            complement(1337..2539)
                     /locus_tag="VGC27_12750"
     CDS             complement(1337..2539)
                     /locus_tag="VGC27_12750"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEY0283480.1"
                     /translation="MKFGIDRLLADPALRAPLKGRRVALLAHPASVTENLTHSLDALA
                     ACSDIKLAAAFGPQHGLRGDKQDNMVESPDFSDPVHGIPVFSLYGEVRKPTPAMMDTF
                     DVLLVDLQDLGCRIYTFVTTLFYVLEAAAKHGKAVWVLDRPNPVGRPVEGLRLREGWE
                     SFVGAGLLPTRHGLTLGELGSWFVATHKFDVEYRVIEMEGWRPEAAPGFGWPLGERSW
                     INPSPNAPNLWMARAYPGTVMLEGTALSEGRGTTRPLELFGAPDIDAKRVVAEMHALA
                     PPWLLGCRLRKCWFEPVFHKHAGKLCNGVQIHTEDPGYDHAAFKPWRLQALAFKAIRR
                     LYPDYPLWRDFPYEYEQNRLAIDVINGSSLLREWVDDAATNPQDLDALAVPDEQAWLS
                     ERQAYLRY"
     gene            complement(2536..2979)
                     /locus_tag="VGC27_12755"
     CDS             complement(2536..2979)
                     /locus_tag="VGC27_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEY0283481.1"
                     /translation="MAGIGGLVIPSPAASTRADELWRHTCFEAFLRPSPGEAYVEFNF
                     SPSTQWAAYRFSGVRSGRRNTVEIGAPSIELRRNAECFELRVHLKLEGLPDFPGDSAW
                     RLGLSAVVEEAGGRQSYWALAHPPGKADFHHSDCFALELPAAWQV"
     gene            complement(3186..>4205)
                     /locus_tag="VGC27_12760"
     CDS             complement(3186..>4205)
                     /locus_tag="VGC27_12760"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283482.1"
                     /translation="KTQELRPSLAIVDKKGKIITMPNVGEVRYPLSMDAILSVEDGSE
                     VRAGDVLARIPTEGAKTRDITGGLPRVAELFEARKPKEAAVLAELDGFVEFGKDYKNK
                     RRVIVKPKNDKIEPTEYLIPKGKHISVREGDYVEKGDYIVEGNPSPHDILRIKGVEEL
                     ATYLVKEIQDVYRLQGVKINDKHIEVIVRQMMQKTEITDGGDTTLLAGEQVDQLELDE
                     ANQKAVDQSGKAAEGRPVLLGITKASLQTRSVFSAASFQETTRVLTDAAVNGKVDTLE
                     GLKENVIVGRLIPAGTGGAIARLRHIAAERDRVIQAEQASKAPPAQIEGPAPAAPDTP
                     AAAAE"
BASE COUNT          755 a         1375 c         1257 g          818 t
ORIGIN      
        1 ccgtatcgat gcccatcgcg gtcatgcgcg cgtcgagcac gtcgaggcgt tcctctcgct
       61 ggctgccgcc gatgatctcg ccgatgcctg gcgcgagcac atccatcgcc gcgacggttt
      121 tgccgtcgtc gttgacgcgc atgtagaacg ccttgatgtc cttcgggtag ttcatcacga
      181 tgaccggctt cctggcgtgc ttttcggtga gatagcgctc gtgctccgac tggagatcaa
      241 cgccccactt gacgggaaac tcgaacttct ccttcgaccg ctccagcacg gcgaccgcct
      301 cgctgtagtc catgcgcacg aattcggagc cgacgattcc ctccagcttc gccaccagcc
      361 ccttctcgat ccgttcgtcg aagaaggcga gatcttcgtg cctttccttc agcagcgtct
      421 cgaaggcgga cttcaacaac cgttcggcca gcgtcgcatc gtccgaaagg tccgcgaaag
      481 cgatctccgg ctcgaccatc cagaactcgg cgagatgacg gctggtgttc gagttctcgg
      541 cgcggaacgt cggcccgaag gtgtagacct tggtgagcgc gagacaatat gcctctacgt
      601 tcaattgccc cgataccgtc aggaacgcgt cgcggccgaa gaagtccttc gagaaatcca
      661 ctttgccttc cggcgtgcgc ggcagattgg cgaaatcgag cgtcgagacc cggaacatct
      721 ccccggcccc ttccgcatcg gacgacgtga tgatcggcgt gttgacccag aaaaaaccgt
      781 tctcatcgaa gaagcgatgg atcgcttgcg ccagcgtgtg ccttacccgc gtcgccgccc
      841 cgatcacgtt cgtccgcggc cgcaggtgcg ccacctcgcg caggaactcc aaagagtggg
      901 gcttgggctg gatcgggtag gtatccggat cttccaccca gccgatcact ttcacggcgc
      961 tcgcctgcat ctcgaaaggc tggcccttcg acggcgacgg tacgatcatt cccaccgctt
     1021 ccaccgcgca accggccgta agcttcagaa cttcatcggt gtaattcggc agcgtgttcg
     1081 gcacgaccac ctgcaccgga tggaacgtcg agccgtccga tacctgcaaa aacgaaatgc
     1141 cagctttcga gtcccgccgg gtgcgtaccc agcctttgac cgcgaccggc gcatccttcg
     1201 gcgcttcgcc ggccaggatc gcgcggacgc tgaattcttt cttatccatt cggcgagagt
     1261 tacccagtcg gattggcctt gtccagggct tcccgtgatc gacgcgccgc ggatttcggc
     1321 acgtaacgcg agtggttcaa tagcgcaggt atgcctggcg ctcgctcaac cacgcctgtt
     1381 cgtccggcac ggccagcgcg tccaggtctt gcggatttgt tgctgcgtcg tccacccatt
     1441 cccgcagcaa cgacgagccg ttgatgacat cgatggcgag tctgttctgc tcgtattcgt
     1501 aaggaaaatc gcgccacagc ggatagtccg gatacaagcg ccggatcgct ttgaaggcga
     1561 gcgcctggag acgccacggc ttgaacgctg cgtgatcgta accagggtcc tcggtgtgaa
     1621 tctgcacacc gttgcagagc ttgcccgcgt gcttgtgaaa cacaggttcg aaccagcatt
     1681 tgcgtagccg gcagccgagc agccatggcg gcgccagcgc gtgcatctcc gccaccacgc
     1741 gctttgcgtc gatgtctggg gcgccgaaca gttccagcgg ccgggtggtg ccgcggccct
     1801 ccgagagcgc cgtgccttcg agcatcaccg tgcccgggta tgcgcgcgcc atccataaat
     1861 tcggcgcgtt gggactggga ttgatccaac tgcgctcgcc aagcggccag ccgaagcccg
     1921 gcgccgcctc cggccgccaa ccctccatct cgatgacgcg atactcgaca tcgaacttat
     1981 gcgttgcgac gaaccacgaa cctagctcgc cgagcgtgag cccgtgtcgc gtcggcaaga
     2041 gtccggcgcc gacgaagctt tcccagcctt cacgcagtct cagaccctcg acgggacggc
     2101 cgacgggatt gggacggtcc agcacccaca cggcctttcc gtgtttcgcg gccgcttcca
     2161 acacgtaaaa cagcgtcgtg acgaaggtgt aaatgcggca gcccaaatcc tgtagatcga
     2221 cgagaaggac atcgaacgtg tccatcatcg caggcgtcgg ctttcgcact tctccgtaca
     2281 ggctgaagac cggaatgcca tggacaggat cgctgaagtc cggcgactcg accatgttgt
     2341 cttgcttatc gcctcgcaga ccgtgctgcg gcccgaaggc ggcggcgagc tttatgtcgc
     2401 tgcatgcggc cagcgcatcg agcgaatggg tcagattttc ggtgaccgac gcgggatgcg
     2461 cgagcaacgc cacgcgcctg cccttcagcg gcgcgcgcaa agcagggtcg gcgagaagcc
     2521 ggtcgatgcc gaatttcata cctgccacgc tgccggaagt tcgagcgcaa aacaatcgga
     2581 atgatggaaa tcggccttgc ccggcggatg cgccagcgcc caataggatt ggcggccgcc
     2641 ggcttcttcg actaccgccg aaaggccgag gcgccatgcc gaatcgccag gaaaatcagg
     2701 caacccctcc agcttcaaat ggacacgcaa ttcaaagcac tcggcgttgc gcctcaactc
     2761 tatcgacgga gcgccgatct cgacggtatt acgcctcccg ctccggacgc cgctgaaccg
     2821 ataagcggcc cattgcgtcg agggagaaaa gttgaattcg acatacgcct cgcccggcga
     2881 tgggcgcagg aacgcctcga agcaagtgtg ccgccaaagc tcatcggcgc gcgtcgatgc
     2941 ggcaggggac gggatgacga gaccgccgat gccggccaag taataatgca gcagcaaatt
     3001 gccgcgccgg gaacggacgg cgtagacttc gatgcgtgtc gccgcagcgc aacgcgagtc
     3061 cggatgcagc ttcaatgctt ggcgcatacg gacattgtag catctccggc gggggcaaaa
     3121 aggaagggcc ggtctcgcga ccggcccttt ttcgattttc cccttcatgg ggagaacagg
     3181 ttcaactact cggccgccgc agccggtgta tcgggtgcag caggcgccgg accctcgatc
     3241 tgtgccggag gggccttgct ggcctgctcc gcctggatca cgcgatcgcg ttcggccgcg
     3301 atgtgccgca gccgcgcgat ggctccgccc gttcccgccg ggatcaggcg gccaacgatc
     3361 acgttttcct tgagaccttc cagcgtatcg accttgccgt tgacggcggc gtcggtgagg
     3421 acgcgcgtcg tttcctggaa ggacgccgcc gagaacaccg aacgcgtctg caacgacgcc
     3481 ttggtgatgc ccagcagaac aggccggccc tcggcggcct tgccgctttg gtcgacagcc
     3541 ttctgattgg cctcgtccag ctccaactgg tccacctgtt cgccggcgag cagagttgtg
     3601 tcgccgccgt cggttatttc cgtcttctgc atcatctgac ggacgatcac ttcgatgtgc
     3661 ttgtcgttga tcttcacgcc ctgcaaccgg tagacgtcct ggatttcctt caccaggtag
     3721 gtcgcaagct cctcaacgcc cttgatgcgc aggatgtcat gcggcgaagg attgccttcg
     3781 acgatgtaat cgcccttctc gacgtaatcc ccctcgcgca cggaaatgtg cttgcctttg
     3841 gggatcaggt attcggtcgg ttcgattttg tcgttcttcg gcttgacgat gacgcggcgc
     3901 ttgttcttgt agtccttgcc gaactcgacg aacccgtcca gttccgccag caccgccgct
     3961 tccttcggct tgcgcgcctc gaacagctcc gccacgcgcg gcaggccgcc cgtgatgtcg
     4021 cgcgttttgg cgccttccgt cgggatgcgg gcgagcacgt cgccggcgcg cacctcggac
     4081 ccgtcctcga ccgagaggat ggcgtccatc gaaagcggat acctcacctc gccgacgttc
     4141 ggcatggtga tgatcttgcc cttcttgtcg acgatcgcga gactgggacg aagctcctgc
     4201 gtctt
//
LOCUS       DASWPU010000601         9694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_109685,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000601 DASWPU010000000
VERSION     DASWPU010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109685"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2183
                     /locus_tag="VGC27_12765"
     CDS             <1..2183
                     /locus_tag="VGC27_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEY0283483.1"
                     /translation="ATEPAGFQEIAAETDSSNGEKLEHEETRPEADTQGADSEAAQPL
                     ESARPTQRWMRKRHYKIQEVIKRRQVILVQVVKEERGNKGAALTTYLSLAGRYCVLMP
                     NTPRGGGISRKITNAADRKRLKSAAQALELPEGMGLIIRTAGENRTKVEIKRDYEYLL
                     RLWDTIRELTLKSNAPACVHEEGDLIKRAIRDLYNKDVSEVIVEGDEGYRNAKDFMRM
                     LMPSHAKNVKPFKEPLPLFQRYHIEAQLDAMFSPTVTLKSGGYIVINQTEALVSIDVN
                     SGRATREHSIEETATKTNLEAADEIGRQLRLRDLAGLIVIDFIDMEDSRNDRAVEKRM
                     HNAVKNDRARVQLGKISQFGLLEMSRQRLRAGVIAGSTVPCPHCGGQGIVRSVESTSL
                     RVLRGLEEEAQKQRADGLIVRVATEVAIYTLNQKRRELTRLENEYDMTISFDPKEDLK
                     AGDFDIERIGQRDPETRPKPPPPVEDAPPVMEEEIEPEIVEDSEPVPAEDTIHEEIPA
                     ESHHHLQQGEEGSGRRRRRRRRRGGRDRDRPVQGEQRPEAGQPVPQIAEDSGVQETYN
                     GDAAGELVEADAPLLQAPPNGEGQQTLQQGPFGEGPRRKRRRRRGRRGGRDRENSGGR
                     TDNGAPSAVEGTMENRFGQPDEIDTTPRDEPKRPVEIQPARGPEAIPNAASTPVWSLK
                     GERPEIPPQQEQEKVSAEAEAPRETSASPQPAKKGWWQRAFRSD"
     gene            complement(2204..3565)
                     /locus_tag="VGC27_12770"
     CDS             complement(2204..3565)
                     /locus_tag="VGC27_12770"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0283484.1"
                     /translation="MFCSRIVAGTAGFFLLAACSIDLGGSLGLDGSGKTTAQVVGDEP
                     FAVKVGAATLAQGGNAVDAVTAMYFALSVTYPVAAGLGGGGICIVHDPAHNQSEEFDF
                     LARDAAGGGAYAVPGNVRGFALMQSTYGALPWQKVVAPGEGYARAGFPISRALATRLA
                     EAADVIRLDAGLAAEFMDESGRLKSTGSVATNAELAETLATIRTQGAEAFYTGAIAGK
                     IVAYSASQGGSITQADMAAYPAVRATPRVIQMGRDYVFLPASRTGAGAFAGALFDNLS
                     RARATPAGMRDAEAAVITAVKQTLSGFGLNSLPKDLGATGFAAIDTRGQAVACAVTMN
                     GPFGSGHTALGTGVALARAPSSGMVGLASAFLTPVIASPSGDRPATLAGAGAGGPNGS
                     AAIAYALTRITHGEEILSRKDLRSTGVAPYDTVNAIVCTSSICAALSDPAASGLGATV
                     PGR"
     gene            3810..5069
                     /locus_tag="VGC27_12775"
     CDS             3810..5069
                     /locus_tag="VGC27_12775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0283485.1"
                     /translation="MLQDTETERLLQSYETPILRVAGIDPAAVKMYIVNDTGLNAFVA
                     EGQNIFVNAGLFIQLQNPNELIGVLAHETGHMAGGHLIRGSDAIAKASIPMLLSMVVG
                     MAAAVAGAGEAGMVLMGVGQSAAQAQFMQFSRVQEATADKMGQRFLRQTHQSGLGMVN
                     VFNRMANDAARAGINEKEFASTHPADRDRVALMQKEADASPYRDVPDSPASVHAFEMI
                     KAKVIGFLLPVRDVFYRYPLSDTSAPARYARAMVYMRQPDLPKALAEINSLIKDEPKN
                     PYFHEVLGQIYVSMSQPLKGIAPYQAAVDLLPDAPQLRISLAAAQIATERKEIEKIAI
                     EHLKIALQQDSDSPFGWYEMAQAYSDLGNEPMANLATAERYYTFGALQQAAVFAGRAR
                     QKLTKGSTEWERANDIISVAATQPRRR"
     gene            5087..5824
                     /locus_tag="VGC27_12780"
     CDS             5087..5824
                     /locus_tag="VGC27_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0283486.1"
                     /translation="MNEQWKAAIGGGAIGAVLSLACVFAAGQFGFLPGTTDARLHSYL
                     VAHPYLLVEMTEKLQKAEAEKEQQGRQAAIDKVGLKRLFDPSVAYVTGPANPKNTFVE
                     FFDYNCVHCRNSFAAVKKFYDAHKGDTRFAFIEYPVFGDASNAAARVAVAARQQSDRY
                     LALHFLLMSEKNAIDDATLYAGAKKAGLDMAKLSAALLAPDLTRPIVAAHKIGEQAKI
                     DGTPTFVINGKLHIGEITEADFKRLTR"
     gene            complement(5838..6833)
                     /gene="thiS"
                     /locus_tag="VGC27_12785"
     CDS             complement(5838..6833)
                     /gene="thiS"
                     /locus_tag="VGC27_12785"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY0283487.1"
                     /translation="MAVTLTINGETRHLGGSVSVAVLLTGMGLDPAKIAVERNQEIVP
                     RSQYSGTMVADGDKLEIVQFIGGGNALPPEPVDKPFVVAGRTFRSRLIVGTGKYKTYA
                     QNAAALEASGAEIVTVAVRRVNLTDPDQPKLVDFIDPKKYTYLPNTAGCFTGEDAVRT
                     LRLAREAGGWTLVKLEVLGDKKTLYPNMIETLRATELLLNEGFQVMVYCSDDPLMAKR
                     LEELGACAIMPLAAPIGSGLGVQNPVGIRMIVEEAKVPVIVDAGVGTASDATVAMELG
                     CDAVLMNTAIAEAKDPVMMAGAMRHAVEAGRLAYLAGRMAKRRYADPSSPLAGLI"
     gene            6916..7380
                     /gene="aroQ"
                     /locus_tag="VGC27_12790"
     CDS             6916..7380
                     /gene="aroQ"
                     /locus_tag="VGC27_12790"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY0283488.1"
                     /translation="MAKKSKTLPIYVLSGPNLNLLGLREPEIYGRTTLAEIGRMVTKR
                     AKSHGLSVVFHQSNHEGDLIDWVQEARTGSAGLIVNGGAYTHTSVALHDALRAVDRPV
                     VEVHLSNPFAREEFRHHSLISPLAKGVIVGFGALSYVLAVDALAALIESSGV"
     gene            7383..7877
                     /gene="accB"
                     /locus_tag="VGC27_12795"
     CDS             7383..7877
                     /gene="accB"
                     /locus_tag="VGC27_12795"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY0283489.1"
                     /translation="MKDVKSIGARIAAGGKAKPTPPIDAAAIRELADLLAETGLTEIE
                     IEHGGARLRVARQRESTFVASAAAPVAASGAPVADAPAAKGPPAGAVTSPMVGTVYVA
                     PEPGKPPFVKAGDQVQEGDTLLIVEAMKTMNPILSPRGGKVTEILIHDAEPVEFGQPL
                     LVIA"
     gene            7974..9320
                     /gene="accC"
                     /locus_tag="VGC27_12800"
     CDS             7974..9320
                     /gene="accC"
                     /locus_tag="VGC27_12800"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY0283490.1"
                     /translation="MFEKILIANRGEIALRITRACKEMGIATVAVHSTADADAMHVRL
                     ADESVCIGPPPAAQSYLNIPAIISACEITGAQAIHPGYGFLSENARFVEIVKEHGITF
                     IGPEPEHIRLMGDKIAAIEAVKALGIPTVPGSNGAVGSDEEAARIAAGIGYPVLIKAT
                     AGGGGRGMKVAEKPSDLSLALSTARAEAKAAFGNDAVYMEKYLQKPRHIEVQVLADSA
                     GHVVYLGERDCSLQRRHQKVWEEAGSPALNAAQRDEIGAIVTKALEKLGYLGAGTVEF
                     LYEDGRFYFIEMNTRLQVEHPVSEAITGIDIVREQIRIASGAPLDVEQKDIEFSGHAI
                     ECRINAENAFTFQPSPGLITQFHTPGGLGVRFDSAIYAGYRIPPYYDSLAGKLIVHGR
                     TRNECLMRLRRALDELVVGGIDTTIPLHQQLVRERDIIDGNYNIHWLEKFLAKKGA"
     gene            9392..9694
                     /locus_tag="VGC27_12805"
                     /pseudo
     CDS             9392..9694
                     /locus_tag="VGC27_12805"
                     /inference="COORDINATES: protein motif:HMM:NF015546.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
BASE COUNT         1935 a         3236 c         2963 g         1560 t
ORIGIN      
        1 aagcgaccga accggccggt ttccaagaaa tcgcggcaga aacggattcg agcaacggcg
       61 aaaagctcga acacgaagag acgcggccgg aggccgatac gcaaggcgcg gactccgagg
      121 ccgcacagcc gctggagtcc gcccgtccga cacagcgctg gatgcgcaag cgccattaca
      181 agatccagga agtcatcaag cgccgccagg tcattttggt gcaggtggtc aaagaagagc
      241 gcggcaacaa gggcgcggcc cttacgacct atctgtcgct ggccgggcgc tattgcgtgc
      301 tcatgccgaa tacgccgcgc ggcggcggca tcagccgcaa gatcaccaat gccgccgacc
      361 gcaagcggct gaaatcagcg gcccaggcgc tcgaactgcc cgaaggcatg ggtctcatca
      421 tccgcaccgc gggcgagaac cggaccaagg tcgagatcaa gcgcgactac gaatacctgc
      481 tccgcttgtg ggacacgatc cgcgagctga cgctgaaatc caacgcgccg gcctgcgtcc
      541 acgaggaagg cgacctgatc aagcgcgcca tccgcgacct ctacaacaag gacgtgtccg
      601 aggtcatcgt cgagggcgac gaaggctatc gcaacgccaa agacttcatg cggatgctga
      661 tgccgtcgca tgccaagaac gttaagccct tcaaggaacc gctgcctctc ttccagcgct
      721 accatatcga ggcgcaactc gacgcgatgt tctctccgac cgtgacgctc aagtccgggg
      781 gctacatcgt catcaaccag accgaagcgc tggtctccat cgacgtcaat tcggggcgcg
      841 cgacccgcga gcattcgatc gaggaaaccg ccaccaagac caaccttgaa gccgccgacg
      901 agatcggccg ccagttgcgt ttgcgcgacc tggccggcct catcgtcatc gacttcatcg
      961 acatggaaga cagccgcaac gaccgcgccg tcgaaaagcg gatgcacaac gcggtgaaga
     1021 acgaccgcgc ccgggtgcag ttgggcaaga tcagccagtt cggactgctc gagatgtcgc
     1081 gccagcgcct gcgcgccggc gtcatcgccg gctcgaccgt gccctgcccg cattgcggcg
     1141 gccaaggcat cgtgcgctcc gtggaatcca cctcgctgcg cgtgctgcgc ggcttggagg
     1201 aggaagccca gaaacagcgc gcggacggcc tgatcgtgcg cgtcgcgacc gaggtggcca
     1261 tctacacgct caaccagaag cgccgcgaac tgacgcgcct ggaaaacgaa tacgacatga
     1321 ccatcagctt cgatcccaaa gaggatctca aggccggcga tttcgacatc gagcgcatcg
     1381 gtcagcgcga cccggaaacc cgtccgaagc cgccgccgcc cgtcgaggac gccccgccgg
     1441 tcatggaaga ggaaatcgag ccggaaatcg tcgaagacag cgagccggtt cctgccgaag
     1501 acacgatcca cgaagaaatt ccggcggaat cccatcatca tctgcaacag ggcgaagaag
     1561 gcagcggccg ccgcaggcgt cgccgccgcc gccgcggcgg gcgcgatcgc gaccgtccgg
     1621 tccagggcga gcagaggccg gaagccggcc agcctgttcc gcaaatcgcg gaggattccg
     1681 gcgtccagga aacctacaac ggcgatgcgg cgggcgaact cgtcgaagcg gacgctccgt
     1741 tgttgcaggc gcccccgaac ggcgaaggcc agcaaaccct gcagcaaggt ccgttcggcg
     1801 aaggccctcg ccgcaagcgc cggcgcaggc ggggacgccg cggcggacgc gatcgggaaa
     1861 attccggcgg ccgcacggac aatggtgcgc caagtgccgt tgagggtacg atggaaaatc
     1921 gcttcggcca accggacgaa atcgatacga cgccgcgcga cgaaccgaaa cgtccggtgg
     1981 aaattcaacc ggcccgcggc ccggaagcca ttcccaacgc cgcttccacc ccggtctggt
     2041 cgctgaaagg cgaacgcccc gaaatcccgc cgcagcagga acaggaaaag gtctctgcag
     2101 aggccgaagc gccgcgtgaa acgtccgcct cgccgcaacc cgccaagaag ggttggtggc
     2161 aacgggcttt ccgttcggac tgacggacca aaagactctt cggctaccgg cccggcacag
     2221 tggcgcccaa gccgctggcg gcgggatcgg acaacgcggc gcagatactg ctggtacaga
     2281 cgatggcgtt caccgtgtcg tacggcgcca cccccgttga gcgcagatct ttgcggctca
     2341 gaatttcctc gccatgcgtg atgcgcgtca acgcataggc aatagccgcg ctgccgttgg
     2401 gtccgcccgc tccggcgccg gcaagcgtcg ccggccggtc gccgctgggg ctggcgatca
     2461 caggagtgag aaacgcggaa gccagaccca ccatacccga agagggcgca cgcgccaggg
     2521 cgacgccggt gccaagcgcc gtgtgacccg atccgaaggg accgttcatc gtcaccgcac
     2581 aggctaccgc ctgccccctc gtatcgatgg cggcgaagcc cgtggcgccg aggtccttgg
     2641 gcagactgtt gaggccgaag ccgctgagcg tctgcttgac ggcggtgatc accgcagcct
     2701 cggcgtctcg catgccggcg ggcgtcgccc gcgcacgaga cagattgtcg aacagcgcac
     2761 cggcaaacgc tccggccccg gtacgggagg ccggaaggaa cacatagtcg cggcccatct
     2821 gtatcacgcg cggcgttgcg cgcacggcag gataagccgc catgtcggcc tgtgtgatgg
     2881 aaccgccttg cgatgcggaa taagccacga tcttgccggc gatggccccc gtataaaatg
     2941 cttccgcgcc ctgcgtgcgg atcgtcgcca gcgtctccgc cagctcggcg ttggtcgcga
     3001 cgcttccggt gctcttgagg cgtcccgatt catccatgaa ttccgccgca aggccggcgt
     3061 cgaggcggat tacgtccgcc gcttccgcca gacgggtagc cagagcgcgc gaaatcggaa
     3121 agcccgcgcg cgcgtagcct tcacccggcg caaccacctt ctgccaggga agcgcgccat
     3181 aggtgctttg catcagtgca aaaccgcgga cgttgcccgg gaccgcgtat gctcctcccc
     3241 ccgccgcatc gcgcgccaga aaatcgaatt cttcactctg attgtgcgcc ggatcgtgaa
     3301 cgatgcagat gccgccgccg ccaaggccgg ccgcaaccgg ataggtgacg gacaacgcga
     3361 aatacatcgc cgtcacagca tcgactgcgt tgccgccctg cgccagcgtg gccgccccca
     3421 ctttcacggc aaagggttcg tcgccgacaa cttgcgccgt ggtcttgccc gatccgtcga
     3481 gaccaagcga cccgccgaga tcgatcgaac atgctgcgag cagaaaaaag ccggccgtgc
     3541 cggcaacgat ccgtgagcaa aacatgaacc gggtgtaatc cttggctggt tattaacgaa
     3601 acggcacttc cgcgtccgtt gacgcaagcg tgagaggcac ctaccgtctc ctcatgggtt
     3661 ccggggtgta gaggagagtt caagccttgt cgatttgcaa ccctttgcgg cgaatcctac
     3721 cggcttcagg gcgtgtactt gcctttcttg cgggcttctt aggcgcgctc ggcgccttcg
     3781 cgacaccggc tttcgctcag gccatttcct tgttgcagga taccgagacc gagaggctgc
     3841 tgcaatccta cgaaacaccg atcctgcgcg tcgcgggcat cgaccctgcc gccgtcaaga
     3901 tgtatatcgt caacgatacc gggttaaacg cctttgtcgc cgaaggacag aatattttcg
     3961 tcaacgctgg gttgttcatc caactccaga atcccaacga gctcatcggc gtcctggcgc
     4021 acgaaaccgg gcacatggcc ggggggcatc tgatccgcgg ctccgacgcc atcgccaagg
     4081 cctcgatccc catgctgctg tcgatggtcg tgggaatggc cgccgccgtc gccggagcgg
     4141 gcgaagcggg catggtcctg atgggggttg gccaaagcgc cgcacaggcc caattcatgc
     4201 agttcagtcg cgttcaggaa gccaccgccg acaagatggg acaaaggttc ctgcgccaga
     4261 cgcatcagtc cgggctgggc atggtgaacg tgttcaatcg catggccaac gacgccgcca
     4321 gagcgggcat caacgagaag gaattcgcct ccacccaccc ggcggaccgc gaccgcgtcg
     4381 ccctgatgca gaaagaggcg gatgcctctc cgtatcgcga cgtgcccgac agtccggcat
     4441 ccgtccatgc ctttgaaatg atcaaggcca aagtgatcgg cttcctgctg ccggtgcgcg
     4501 acgtgttcta tcgctatccg ctgtcggaca cgagcgcgcc tgcccgctac gcccgcgcca
     4561 tggtctatat gcgccagccg gatcttccca aggcgctcgc ggaaatcaac agcttgatca
     4621 aggatgaacc caagaacccg tacttccacg aggtgctggg ccagatctac gtctcgatga
     4681 gccagccctt aaagggcatt gcgccctatc aggccgcggt ggatctcttg ccggacgcgc
     4741 cccaactgcg catctccctg gccgcggcgc aaatcgccac agaaagaaaa gagatcgaga
     4801 aaatcgcgat cgagcatctc aagatcgctt tgcagcagga cagcgactcg cctttcggat
     4861 ggtacgaaat ggcccaagcc tacagcgatc tgggcaacga accgatggca aatctggcga
     4921 ccgcggaacg ctattatacg ttcggcgccc tgcagcaggc ggccgttttc gctggacgcg
     4981 caagacagaa gctcaccaaa ggctccacgg aatgggagcg ggcaaacgac atcatatccg
     5041 tggcggcaac ccagccgcga cgacggtaac cgcgggaata aatccgatga acgaacaatg
     5101 gaaagcggcc atcggcggcg gcgcgatcgg tgcggtcttg tcgttggctt gcgtgttcgc
     5161 ggccggtcag ttcggctttc ttccaggcac cacggacgcc aggctccaca gttatctggt
     5221 ggcgcatcct tatctgctgg tcgagatgac ggaaaagctg cagaaggcgg aggcggaaaa
     5281 ggagcagcaa gggcgccaag cggcgatcga caaggtggga ctgaagcggc tattcgatcc
     5341 ttccgtcgct tatgtcaccg gccccgccaa cccgaagaat accttcgtcg agtttttcga
     5401 ctataactgc gttcactgtc gcaactcgtt cgcggcggtg aagaaattct acgacgccca
     5461 caaaggcgac acgcgcttcg cgtttatcga atacccggtc ttcggagacg cctcaaacgc
     5521 ggcggcgcgc gtggccgttg ccgcccgcca gcagagcgac cgatatcttg ccctgcactt
     5581 tctcctgatg agcgaaaaga acgcaatcga cgacgcgacg ctgtatgccg gcgccaaaaa
     5641 ggcgggcctc gacatggcca agctcagcgc cgccctgctt gccccggatc tcacccggcc
     5701 gatcgtcgcc gcgcacaaga tcggcgagca ggcgaagatc gacggcacgc cgacgttcgt
     5761 tatcaacggc aagctgcaca tcggcgaaat caccgaagcc gatttcaaga ggctgacgcg
     5821 ctgaagccgc cgcggcgtca aatcaatccc gccagcgggc tcgacggatc ggcgtagcgc
     5881 cgtttcgcca tgcgccccgc gagataagcg agacgcccgg cttccaccgc atggcgcatc
     5941 gcgcccgcca tcatgaccgg gtccttggct tcggcgatgg ccgtgttcat cagcacggcg
     6001 tcgcaaccca gttccatcgc caccgtggcg tccgaggccg tgcccacgcc cgcatcgaca
     6061 atcaccggca ctttcgcttc ctcgacgatc atgcggatgc cgaccggatt ctgcacgccc
     6121 agccccgatc cgatgggcgc ggcgagcggc atgatcgcgc acgcccccag ctcttcgagc
     6181 cgcttggcca tcaggggatc gtcgctgcaa tagaccatca cctggaagcc ctcgttgagg
     6241 agaagctcgg tggcgcgcag ggtctcgatc atgttgggat aaagcgtctt cttgtcgccc
     6301 agcacttcca gcttcaccag cgtccagccg ccggcctcgc gcgccagccg cagggtgcgc
     6361 accgcgtcct cgccggtgaa gcatccggcg gtgttcggca ggtaggtgta tttcttgggg
     6421 tcgatgaaat cgaccagctt gggctgatcg ggatcggtca ggttcacccg gcgcaccgcg
     6481 acggtgacga tctccgcccc gctggcctcc agcgccgccg cgttctgggc ataggtcttg
     6541 tacttgccgg tgccgacgat caggcgggaa cggaaggtcc tgccggcgac gacgaagggt
     6601 ttatccacag gctcgggcgg cagcgcgttg cctcccccga tgaactggac gatctcaagc
     6661 ttgtcgccgt ccgccaccat cgtcccgcta tactgggaac gcggcacgat ctcctggttg
     6721 cgttccaccg cgatcttggc ggggtcgagc cccatgccgg tcagaagcac ggctacgctg
     6781 acggaaccgc caagatgccg ggtttccccg ttaatagtca gtgtcacggc cataaagcct
     6841 gttcccgatt ggttgtttgc ctaacgtcca aggcccagta taaagcccgg atcgacgaag
     6901 ttaacggagc ccttcgtggc caagaaatcc aaaacgctgc cgatatatgt cctgagcggc
     6961 ccgaacctca accttctggg cctgcgcgaa ccggaaattt atggtcgcac cacattggcc
     7021 gaaatcggcc ggatggtcac caaacgggca aaaagccacg gactttccgt cgttttccac
     7081 caatccaacc atgaaggcga tctgatcgac tgggttcagg aagcccgcac cggctccgcc
     7141 ggactgatcg tcaacggcgg ggcttatacg catacctccg ttgcgctgca cgacgctctg
     7201 cgggccgtgg accggccggt ggtggaagtc catctctcca atccgttcgc gcgggaggaa
     7261 ttccgccatc attcgctcat cagcccgctt gccaaaggcg tgatcgtcgg attcggcgcg
     7321 ctgagttatg tattggcggt ggacgcactg gccgccctca tcgaaagctc cggcgtatga
     7381 ccatgaagga cgtaaaatcg atcggcgccc gcattgcggc gggcggcaaa gcaaaaccca
     7441 cgccgcccat cgacgccgcg gccattcgcg aactggccga cctgctcgcc gagaccggcc
     7501 tcacggagat cgagatcgaa catggcggcg cgcgattgcg cgtggcgcgc cagcgggaaa
     7561 gcacattcgt cgcttccgcc gccgcgcccg ttgccgcgtc cggcgcgccg gttgcggacg
     7621 ctcccgccgc caaaggcccg ccggccggcg ccgtcacctc gcccatggtc ggcaccgtct
     7681 atgtcgcgcc ggaacccggc aagccgcctt tcgtcaaagc gggagatcag gttcaagaag
     7741 gcgatacgct tttgatcgtc gaagcgatga agacgatgaa cccgatcctc agcccgcgcg
     7801 gcggcaaggt gaccgaaatc ctcatacacg acgccgagcc ggtcgagttc ggccagcctt
     7861 tgctcgtgat tgcataatgc cgatgaacat cactattgcc ccccacccgg ctcgctactg
     7921 gcgcgccgtc ggtacgctgt cgctaccgac ccccacaagg gggagataag tcggtgttcg
     7981 aaaaaatcct catcgcgaac cgtggcgaaa tcgcgctgcg catcacccgt gcctgcaagg
     8041 aaatggggat cgccaccgtc gcggtgcatt ccaccgccga cgccgacgcc atgcatgtgc
     8101 gcctcgccga cgagagcgtc tgcatcggcc cgccgcccgc cgcccagtcc tatctcaaca
     8161 ttccggcgat catctcggcc tgcgaaatca ccggcgcgca ggcgatccat cccggctacg
     8221 gcttcctgtc ggagaatgcg cgcttcgtgg agatcgtgaa ggaacacggc atcaccttca
     8281 tcgggccgga gcccgagcat atccgcctga tgggcgacaa gatcgccgcc atagaggccg
     8341 tgaaagcgct cggcattccg acggttcccg gctcgaacgg cgcagtcggc agcgacgaag
     8401 aggccgcgcg catcgccgcc ggcatcggct atcccgtcct catcaaggcg acggcgggcg
     8461 gcggcgggcg cggcatgaaa gtggcggaga aaccgtctga tctgtctttg gcactcagca
     8521 cagcccgcgc ggaggccaag gctgcgttcg gcaacgacgc cgtttatatg gaaaaatatc
     8581 tccagaagcc gcgccacatc gaagtgcagg tgctggcaga ctccgccggc catgtcgtct
     8641 atctgggcga gcgcgactgc tcgctgcagc gccgccacca gaaagtctgg gaagaagcgg
     8701 gcagcccggc gctcaatgcc gcccagcgcg acgagatcgg cgccatcgtc accaaggcgc
     8761 tggaaaaact cggctatctt ggcgccggca cggtcgaatt cctctacgaa gacgggcgct
     8821 tctatttcat cgagatgaac acccgcttgc aggttgagca tccggtcagc gaagcgatca
     8881 ccggcatcga catcgtgcgc gagcagatcc gcatcgccag cggcgcgccg ctcgacgtcg
     8941 aacagaagga catcgaattc tcgggccacg ccatcgaatg ccgcatcaac gccgagaacg
     9001 ccttcacctt ccagccctcg ccgggcctga tcacgcaatt ccacacgccg ggcggcttgg
     9061 gcgtgcgctt cgattccgcg atctatgccg gctatcgcat cccgccctat tacgacagcc
     9121 tggcgggcaa gctcatcgtc cacggccgaa cccgcaacga atgcctgatg cgcctgcgcc
     9181 gggcgctgga cgaactggtg gtcggcggga tcgacaccac cattcccctg catcagcagc
     9241 tcgtgcgcga acgcgacatc atcgacggca actacaatat ccactggctg gagaagttcc
     9301 tggcgaagaa gggggcgtga tgccttctcc tccctttcgt catggccggg tcaagcccgg
     9361 ccatgacgaa gtgtgatacg gtggtgatgc catgcactcg ccgcgcctca cgcccgagat
     9421 cctgcttcgc gcctatgccg aagggctgtt cccgatggcg gagcggcgcg acgatcccaa
     9481 cctcttctgg gtgtcgcccg accggcgggg catttttccc ttggaaagct tccacgttcc
     9541 caagcggctg gcgcgcacgg tgcgctagga caaatttgcg gtcaccagcg acaccgcttt
     9601 cgccgaggtc atgcttgcct gcgccgcgcc cgcgccggac cgcgaagaaa cctggatcaa
     9661 cgacgaaatc ctgcgcctgt acacagcgct gcac
//
LOCUS       DASWPU010000602         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_109691,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000602 DASWPU010000000
VERSION     DASWPU010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109691"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /gene="rpsT"
                     /locus_tag="VGC27_12810"
     CDS             <1..203
                     /gene="rpsT"
                     /locus_tag="VGC27_12810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY0283491.1"
                     /translation="SRKTRIRGLVRKVEEAIEADDPKAALAALKAAEPEIMRGVSKGT
                     VHKNTGSRKVSRLTKRVAKLGK"
     gene            684..1124
                     /locus_tag="VGC27_12815"
     CDS             684..1124
                     /locus_tag="VGC27_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0283492.1"
                     /translation="MASEKLSSGYAGDLKPADAWTLLGSEPTAQLVDVRTRAEWNFVG
                     KPDLSGLGRQVQFVEWLDFPAGTPDPDFVAKASAALGGDKNAPVLFLCRSGARSRAAA
                     TAMTAAGYVRAYNIAEGFEGDPDDNYHRGTKNGWKAAGLPWIQT"
     gene            1261..>2620
                     /gene="dnaA"
                     /locus_tag="VGC27_12820"
     CDS             1261..>2620
                     /gene="dnaA"
                     /locus_tag="VGC27_12820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY0283493.1"
                     /translation="MGQYTNKPTARDWYAVWTCVRERLRHELGDPVFDAWIGPLTLDG
                     VENDELRIGAAKPFVRNWVANHYVTRIERAFAAEKAEPASISIVVSHNPPAVATGISK
                     EPVLVEPQPAPSSYPPRSSEGEEAGAAPKGLWTRMLHPQQTFDSFIPGSANEFGHGAA
                     RAFAEGRGGDIPLLYIHGGFGYGKTHLLNAAALEFRKRGKRVLFLRAEDFMRHFLGAL
                     YRKDTLAFKEELRSADVMIIDDLQHICRSTATASEFLYTVNAFADLRRRVVIAADRGP
                     AALEGLGADIKSRMTGGLVIELGKPDRDTRLAILKSRAAEFTRLRPGVILPDCVLERI
                     ADIDDASPREMIGVLTKLATYADLTKKPVTPEMAEEAVGLRSAPGAKTSIEDIQRKTA
                     EFYKLDVKDFYSPQRARRVARPRQVAMYLARKLTTRSLPEIGRRFGGRDHTTVLHACR
                     RIE"
BASE COUNT          534 a          811 c          822 g          453 t
ORIGIN      
        1 attcccgcaa gacgcgcatt cgcggtctgg tgcgcaaggt cgaagaggcc atcgaagctg
       61 acgatccgaa agcggcactg gcggcgctga aagccgccga acccgaaatc atgcgcggcg
      121 tctccaaagg cacggtccat aagaacaccg gctcgcggaa ggtgtcgcgc ctcaccaagc
      181 gcgtggctaa gctcggcaag taagactagt tctctggatt ctttgcggaa tctggtccag
      241 aatgggtccg aaggcatttc gggcccattt ttatttgtcc gtagactgaa cagcgggaca
      301 aacgacggtt gtgcgatgcg gcaaaaagag ccgtcaccat ctaagtgact gattcgttaa
      361 ggttagtgac cgtcgcgcta ttttccgtat gaagaacata tagttagtac gggtacggag
      421 ataagttttt tcgacataaa accgtcatgt tcctgatttg tccccttgca gtcgaatcag
      481 tcttgcgctt atggttatca ggtggtggca cgagaacaaa aaaatgccaa agagaataag
      541 aaataattcc gcaatattcg ctagttctcg atgtacttct cgagcgagtt gcaagtctag
      601 gctttagtga attacctaaa atgggtgcgt aagcggcgta ggcgagagct tgcgacagcg
      661 atctatttct ctgggctggg gccatggcgt ctgaaaaact ctcttctgga tatgccggag
      721 acctgaaacc ggccgatgcc tggactttgc tcggcagcga gccgacggcc caacttgtag
      781 acgttcgcac ccgtgcggaa tggaattttg tcggcaagcc ggacctgtcc ggtctcggcc
      841 gccaggttca gttcgtcgag tggctggact tccccgccgg tacgccggac cccgacttcg
      901 tggccaaggc cagcgcggcc ttggggggcg acaagaacgc gcccgtgctt ttcctgtgcc
      961 gttcgggcgc gcgctcgcgc gccgcagcga cggccatgac ggcggcgggc tatgtccgcg
     1021 cctataacat cgcggaaggt ttcgaaggcg atcccgacga caactatcac cgcggaacca
     1081 aaaacggctg gaaggccgcc ggccttccct ggatccagac ataggaggag gagatgcgac
     1141 gaagcgaagc gccgcctaga cggcgctcag gaggcggata gaccggacaa cagacacgtt
     1201 cgggggaaca acaatcagaa gagcttgccg tgtcggggcg gacaagtagc gggtgtggat
     1261 atgggacaat acacaaacaa gccgacggca agggactggt atgcggtatg gacatgcgtg
     1321 cgcgagagac tgcgccacga attgggagac ccggtattcg acgcctggat cggcccgctg
     1381 acgctggacg gcgtcgagaa tgacgaactg cgcatcggcg cggccaagcc cttcgtccgc
     1441 aactgggtgg caaaccatta tgtgacgcgc atcgagcgcg ccttcgccgc cgaaaaggcg
     1501 gagcctgctt ccatttccat cgtggtctcc cacaatccgc cggccgtcgc cacgggaatc
     1561 tccaaggaac ccgtgctggt cgagccgcag ccggcgccgt cttcctatcc gccgcgcagc
     1621 agcgaaggcg aagaggccgg cgccgcgccc aagggtctgt ggacgcgcat gctgcatccc
     1681 cagcagacct tcgacagctt cattcccggc agcgccaacg agttcggcca cggcgcggcg
     1741 cgggctttcg cggaaggtcg cggcggcgac attccgctgc tctacatcca tggcggcttc
     1801 ggctacggca aaacgcatct gttgaacgcg gcggcgctcg aattccgcaa gcgcggcaag
     1861 cgcgtgctgt tcctccgcgc cgaggacttc atgcgccact tcctgggcgc gctctatcgc
     1921 aaggacacgc tcgccttcaa ggaagagctg cgcagcgccg acgtgatgat catcgacgat
     1981 ctgcagcaca tctgccgctc caccgcgacg gcctcggagt tcctctacac cgtcaacgct
     2041 ttcgccgatc tgcgccgccg cgtggtgatc gccgccgacc gcggccccgc cgccctagag
     2101 ggcctcggcg ccgacatcaa gtcgcgcatg accggcggac tggtgatcga actcggcaag
     2161 cccgaccgcg atacgcggct ggcgatcctg aaatcgcgcg ccgccgagtt cacccggctg
     2221 cggccgggcg tcatcctgcc ggattgcgtt ctggaacgca tcgccgacat cgacgacgcc
     2281 agcccgcgcg aaatgatcgg tgtactgacg aagctggcga cctatgccga cctgaccaag
     2341 aagccggtga cgccggagat ggccgaggaa gccgtgggcc tgcgcagcgc gccgggcgcc
     2401 aagacctcga tcgaagacat ccagcgcaag accgccgagt tctacaagct cgacgtcaag
     2461 gacttctact cgccgcagcg cgcgcgccgg gtggcgcggc cgcgccaggt ggcgatgtat
     2521 ctcgcccgca agctgacgac gcgatcgctg cccgaaatcg ggcggcgctt cggcggacgc
     2581 gaccacacca ccgtgctgca cgcctgccgg cgcatcgagg
//
LOCUS       DASWPU010000603         7217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_115959,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000603 DASWPU010000000
VERSION     DASWPU010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115959"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1820
                     /locus_tag="VGC27_12825"
     CDS             <1..1820
                     /locus_tag="VGC27_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283494.1"
                     /translation="SCTVLTPQTSPYLPGYFMFHWDNGYYYSGLSGTLTLTDSSGTPR
                     NYNVYFGWTGPWGGYYSTQPMFNYWSPVVLDLDGNGIDLVSANSMSAPWFDMDGDGYR
                     DQTGWVGPNDGILVIDRDGDGLITKGEEISFSRDENGAVSDLEGLRAYDTNQNGMIDA
                     SDAQFSELRVWQDANQDGICEAGELTTLAERSIAAINLTLTPTGNSLDDLTDNVIYGT
                     TQFLRSDGSTGLTGDVALLYHHNGFVMQIGDISRAYHIDPTTRQFIPDDGQPNQIPSE
                     LLGPIVLDLDGDGVSLLSRTSSNVYFDMDKSGIRQRTGWVGPNDGLLVLDRNGDGKIT
                     DGSEISFKDDLPGAVSDLEGLRAYDTNQNGFFDEGDARFSDFRVWRDANQNGVCGSSE
                     LKTLASYGITAINLTQNLTGQTIEGASDNVLYATADVLHGDGSVSGAGDVFLAYDQDP
                     EQPKEISQGEQANLLIQAKQSLRSPWMSFNTPRVSPNGPPQDALPASGANDRDENASG
                     IDAHNPGQPSDGTSPDDRNAPWRTILAAGHGAGTQSALESGLGVVDRKVLQMVSAMST
                     FNSTAPGNLEQAGHRQRPKGMEYLTALPDLHAGTSLHAA"
     gene            1810..2379
                     /locus_tag="VGC27_12830"
     CDS             1810..2379
                     /locus_tag="VGC27_12830"
                     /inference="COORDINATES: protein motif:HMM:NF014813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HEY0283495.1"
                     /translation="MLHEGPGAAMSTPPQPNLPPAGTPRTVAEALGQVVWLLSQSPVH
                     RELRIKDLEWSFMPPILYEQFRIFRFGPLPGLEDPAKLAPAGFDKKSLEQLPLGVAIW
                     AKLSTEAEAKLEKGEHLKPEEWRSGDRVWLVELISPFATPQNKLSEAMLLDLIQGPFK
                     TTPFSLHRTDPKTGRRDKIVMASHLQLQN"
     gene            complement(2462..4042)
                     /locus_tag="VGC27_12835"
     CDS             complement(2462..4042)
                     /locus_tag="VGC27_12835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY0283496.1"
                     /translation="MLSWEPNSRDHNSLESTLFAPNSARLAGAVTPGASRKGDRDPQR
                     AIGGWSQPCCANLFRGALMKPTRHAAWALLWAALSTGPVSAQNQAEMRPQIGLPLSEA
                     LAVAYETNPRLAAAQAGLRATDETVAQANANWRPSINASGTYGIENYKVTGSPSWIKT
                     HPLQVQIGVQENIFRGGRTYAEIGRAKAQVRAGRADLIAAEQTVLLVAATAYMDVIRD
                     SAVVRLRQRNVEVLRQQREATARQFSAGMLTRTDVSQSEARLAAAEAELTAAEGQLAI
                     SRSSFLQAVGRPAETLEENPALPAIIPTSDEAAIAAAIKWNPALISARENERAASYAV
                     DDAIGALLPQVAVQGGYSYSQQDYTSSIGAGSVVHGLSIIGTINVPIYQGGAEEATVR
                     QAKEWHTQARLNVNVMDRQVRDAVATAWNQYRAAEAGIVSNRATAEADEIALVGVRKE
                     QQIGGRTILDVLNAEQELLNAQVTVVASERNRVVAAFQLLAATGNLTAQSLALKVKYY
                     DPLVHYESNATRWIGFGE"
     gene            complement(4429..5169)
                     /locus_tag="VGC27_12840"
     CDS             complement(4429..5169)
                     /locus_tag="VGC27_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0283497.1"
                     /translation="MSLVVESIAKRFERFPALNGVSFVVPQGAFVALLGPSGSGKTTL
                     LRILGGLEFPDAGSVHFADLNWLGVPARHRRAGFVFQNYALFRHMTVAQNIAFGLNVR
                     PRRERPSRSEISRRVEELLALVQLDGLGKRYPSQLSGGQRQRVALARALAIEPRMLLL
                     DEPFGALDAKVRKELRGWLRSLHERLGLTTIFVTHDQEEAFAIADLVAIMNAGKLEQY
                     GTPEDVRRAPKSQFVAEFLDSAQANAFN"
     gene            complement(5181..6035)
                     /gene="cysW"
                     /locus_tag="VGC27_12845"
     CDS             complement(5181..6035)
                     /gene="cysW"
                     /locus_tag="VGC27_12845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEY0283498.1"
                     /translation="MSEVHRRKATQDHPLMRVVLSAVAIGFIGLFLVLPLAMVFTQAL
                     SKGLDFALAAITEPDALAAIRLTLLVAAIAVPLNAVFGIAASWAIAKFDFRGKGFLTT
                     LIDLPFSVSPVVSGLVYVLIFGLQGWFGPELRDHGIKVIFALPGIVVATIFVTFPFVA
                     RELIPLMTDQGRQEEEAAATLGASGFQTFLRVTLPNIKWALFYGVLLCNARAMGEFGA
                     VSVVSGHIRGLTNTMPLHVEVLYNDYQFVGAFAVASLLAMLAIVTLTLKSILEWRYAD
                     EIAAAHRH"
     gene            complement(6032..6880)
                     /gene="cysT"
                     /locus_tag="VGC27_12850"
     CDS             complement(6032..6880)
                     /gene="cysT"
                     /locus_tag="VGC27_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEY0283499.1"
                     /translation="MNTAAASSPLHWRNPSVLPGLGLSLGFALFYLSVIVLIPLAALV
                     IRPWSLGFDGVWEILTDRRVLAALRLSFGGAAIAAGINAVFGFVVAWVLVRYRFPGKR
                     LLDALVDLPFALPTAVAGIALSTLYAPQGWLGEPLSHLGVKVAYTPLGVIVALTFIGL
                     PFVVRTLQPVLANLSLEGEEAAATLGATRLQAIWRVVLPTLLPSLVTGTTLAFARAVG
                     EYGSVIFIAGNLPAISEIAPLLIIIKLEQFDYAGASAIGVIMLAASFILILTLNLLQN
                     WAASRQ"
     gene            complement(6877..>7217)
                     /locus_tag="VGC27_12855"
     CDS             complement(6877..>7217)
                     /locus_tag="VGC27_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0283500.1"
                     /translation="KEYGSDKFQIVYPSNTILAEPPVALVDKNVDRHGTRKVAEAYLQ
                     FLYSKEAQEIEARNFYRPRDPAILAAHAADFPKIPLFTIDDVFGGWQKAQQIHFSDGG
                     VFDQIYKPGK"
BASE COUNT         1484 a         2191 c         2174 g         1368 t
ORIGIN      
        1 cgagttgtac ggtcttaact cctcaaacca gtccttacct gccaggctat ttcatgttcc
       61 attgggacaa cgggtactac tatagcggtc tcagtggcac gctgacgctg acagactcta
      121 gcggaacacc gcgcaactat aatgtctact tcggctggac tggcccgtgg ggcggctact
      181 atagcacgca accgatgttc aactactggt cccctgtcgt cctcgatctt gatggaaacg
      241 gtatcgattt ggtctcggca aacagcatgt cagcgccatg gttcgacatg gacggcgacg
      301 gctatcgcga ccagaccggc tgggtcggtc ccaacgacgg cattctggtc atagaccgcg
      361 acggcgacgg cctaatcacc aaaggcgagg aaataagctt ttcacgggac gagaacggtg
      421 ccgtttccga tcttgaaggc ctgagagcat acgatacaaa ccaaaacggt atgatcgatg
      481 ccagtgacgc acaattctcg gaattgcgtg tctggcagga tgccaatcaa gacggtatat
      541 gcgaagcggg tgagcttact acacttgccg aacgatcgat tgcggctatc aacttgacgc
      601 tgacgccgac cgggaattct ctggacgacc tgacagacaa tgtgatctac ggcacaaccc
      661 agttcctgcg ctccgacggt tcgaccggcc tgacgggcga tgttgccctc ctgtaccatc
      721 acaacggctt tgtgatgcaa atcggtgata tttccagagc ctatcacatc gaccccacaa
      781 caaggcagtt cattccggac gatggacagc ctaaccagat accgtcagaa ctgctcggcc
      841 cgatcgttct tgatctcgac ggcgatggcg tgtcgctgct gtctcgcaca tcatcgaacg
      901 tgtatttcga tatggataag agcggcatcc gtcagaggac cggctgggtt ggacccaacg
      961 acggactgct tgtgctcgac cgcaatggcg atggcaagat caccgacggc tcggaaatct
     1021 ccttcaagga cgatttgccg ggtgcggtct cggaccttga aggcctgcgc gcctacgata
     1081 cgaaccagaa cggcttcttc gacgagggtg atgcaagatt ctcagacttc cgtgtctggc
     1141 gcgatgccaa ccagaatggt gtatgcgggt cgagcgaact taagacgctc gccagctacg
     1201 gcatcaccgc gatcaacctc acgcagaacc tgaccggcca gacgatcgag ggcgcaagcg
     1261 ataatgttct ctatgcaaca gccgatgtac tgcacggcga cggcagtgtg agcggtgcag
     1321 gcgacgtctt cctcgcctac gatcaggacc cggaacagcc taaagaaatt tctcaggggg
     1381 aacaggcgaa tcttctgatc caggccaagc agtcgctgcg gtctccctgg atgtcgttca
     1441 atacgccgcg cgtctcaccc aatgggccgc cgcaagatgc actcccggca agtggggcaa
     1501 acgatcgcga tgaaaacgcc agcggcatcg acgcccataa ccctggtcaa ccgtccgatg
     1561 gtacaagccc cgacgacagg aatgcccctt ggcggaccat cttggccgcg ggtcacggcg
     1621 ccggaactca aagcgcgctc gaatcgggtc ttggggtagt tgaccgcaag gtcctgcaaa
     1681 tggtctcggc gatgtccact ttcaacagca cggcgccggg aaaccttgaa caagctggtc
     1741 acagacagcg tccgaaaggg atggagtact tgaccgcatt gcccgatctg cacgcaggga
     1801 ctagcttgca tgctgcatga aggaccggga gcagccatgt cgacgccgcc gcaaccaaac
     1861 ctacctcctg cgggaacgcc gcgcaccgtc gcggaggcgc tgggtcaggt tgtatggctc
     1921 ctgtcgcagt cgcccgtgca tcgggagttg aggatcaagg atctggaatg gtcgttcatg
     1981 ccgcccattc tttacgaaca gttccgcatc ttccgcttcg gcccgcttcc aggattggag
     2041 gaccccgcaa agctcgcccc ggcaggcttc gacaagaaga gtctggagca gttgccgctt
     2101 ggtgtcgcga tctgggccaa gctttctacc gaagccgaag ccaagctgga gaagggcgag
     2161 catttaaaac cggaagaatg gcgctctggg gatcgggttt ggctggtgga attgatctcg
     2221 cccttcgcca cgcctcaaaa caagctgtcc gaggccatgc tgctcgacct tatccagggt
     2281 ccgttcaaga cgaccccatt ttctctgcac cgcacggatc ccaagacggg tcgccgggac
     2341 aagatcgtca tggcctcgca cctccagctc caaaattgac ctgtgcggaa ggccgcaagg
     2401 catctgtttg ttgcgggtcg gacgaggtgg atgggggagc gggagttcgt cctattctta
     2461 cctattcgcc gaagccgatc cagcgcgtcg cgttgctttc gtaatgtact aaaggatcgt
     2521 agtacttcac cttgagagcg agactctgcg ccgtcagatt gcccgtcgct gcgagaagct
     2581 ggaacgccgc cacgactctg ttgcgttcgg aggccacgac cgtaacctgc gcgttcagca
     2641 attcctgctc tgcgtttaga acgtcgagga ttgtgcgccc tccgatctgc tgctcttttc
     2701 tcacgcccac aagagcaatc tcatcggcct cggcggtcgc acgattggag acgataccgg
     2761 cttcagcggc ccgatattga ttccaggctg tggcgacggc gtcacgcact tgcctgtcca
     2821 tcacattgac attgagccgt gcctgcgtat gccattcttt ggcctgtcgt accgtcgcct
     2881 cttccgcgcc tccctgatag atcggtacgt ttatggtccc gatgatactc aggccgtgca
     2941 ccaccgagcc cgcgccaatg ctcgacgtgt aatcctgctg tgagtaggaa tagccgccct
     3001 ggactgccac ctgcggcagc agcgccccga tggcgtcatc gacagcatag cttgcggccc
     3061 gctcgttttc acgggctgat atcagggcgg ggttccactt gatggccgca gcgatcgctg
     3121 cctcgtcact cgtcgggatg atcgccggca acgccgggtt ttcttccagc gtctccgcgg
     3181 gccggccgac ggcttgcagg aaactggaac ggctgattgc caattggccc tctgcggccg
     3241 ttaactccgc ctcggctgcg gcgagcctgg cttcagattg ggacacatcc gtgcgggtca
     3301 acatcccggc cgagaactgg cgcgcagtcg cttcgcgctg ttggcgcagg acttcgacgt
     3361 tccgctggcg caagcgcaca accgctgaat cgcgtatcac atccatgtag gccgtcgctg
     3421 cgacgaggag cactgtctgt tcggcggcaa tcaaatcggc gcggccggca cggacttgtg
     3481 ctttcgctcg gccgatctcg gcatatgtgc gtccgccccg aaagatattc tcttgcacgc
     3541 ctatctgaac ctgcagcgga tgagtcttga tccagctggg cgagccggtg actttgtaat
     3601 tctcgatacc ataggtgccg ctcgcattga tgctgggccg ccaattggcg ttggcctggg
     3661 caacggtttc atcggtcgcg cgcagccccg cctgagcggc ggccaatcgc gggttcgttt
     3721 cgtacgccac ggctagggcc tccgaaagcg gaaggcctat ttgtggtctc atctcagcct
     3781 gattttgcgc cgaaaccggc cccgtcgata acgcagccca tagcaatgcc catgcggcat
     3841 gacgtgtagg tttcatgagt gccccccgga atagatttgc acagcatggc tgtgaccatc
     3901 ccccaatcgc tcgctgagga tcacggtcgc cctttcggct ggcacctgga gtcactgcgc
     3961 cggcaagtct cgctgaatta ggcgcaaata gtgtgctttc gagcgaattg tgatcacgcg
     4021 agttaggctc ccatgatagc atttttggcc gctcattcac atcgtaccca caggccaaac
     4081 acgggcgctt ccggctgctg cgacatcttg gatgattgcg gccgaacgac cgatggactg
     4141 tggattgggg cgattggcga aatgcgcgaa attgattttg gcgacgcggc gttgagtttc
     4201 tgggatcgcc gccacaggaa cggtttccgg atcggacccg gaaaatcggt ccctcaaata
     4261 cgtattgtgt taaacgtgcc gagcgagatt gccgttcggc gacgcctgtt ccaactgctg
     4321 aacggcatca agttcaagag tgtcgatggc ggcagaacat ctggctcgcg accagacggg
     4381 ccacgttaag gcgccgggcg cggagcccat cgcgcggcaa aggggcggtc agttgaaggc
     4441 gttcgcctgc gcgctgtcca ggaactccgc gacgaattgc gactttgggg cgcgccgcac
     4501 gtcttccggc gtgccgtatt gttcgagctt gccggcgttc atgatcgcga ccagatcggc
     4561 aatcgcgaag gcttcttcct gatcgtgagt gacgaaaatc gtggtcagcc ctagccgctc
     4621 gtgcagcgaa cgcagccagc cgcgcagttc ctttcgcacc ttcgcgtcca acgcgccgaa
     4681 cggctcgtcc agcagcagca tgcgcggctc gatggcaagc gcgcgcgcca gggccacgcg
     4741 ttggcgctgg ccgcctgaga gctggcttgg atagcgtttt cccaagccgt cgagctgcac
     4801 cagcgccagc aattcttcca cccgccggga tatttcgctc cgcgacggcc gctcccggcg
     4861 cggacggacg ttgaggccga aggcgatgtt ctgcgccacc gtcatatgac ggaacaaggc
     4921 gtaattctgg aacacgaacc ccgcccggcg atggcgcgcc ggcaccccca gccagttcag
     4981 atcggcgaag tgaacggatc cggcgtccgg gaattccagt ccgcccagga tgcgcagcag
     5041 cgtcgtcttg cccgacccgg aaggtccgag cagcgctacg aaggcccctt gcggcacgac
     5101 aaagctgacg ccgttcaagg ccggaaagcg ttcgaaccgt tttgcgatgg actcaacgac
     5161 cagcgacatg aattactccg tcaatgtcgg tgcgcggcgg cgatttcatc cgcgtaacgc
     5221 cattcgagaa tggatttgag agtaagcgtg acgatggcga gcatcgccag cagcgacgcc
     5281 accgcaaacg cgccgacgaa ctgatagtcg ttgtagagca cttcgacgtg cagcggcatg
     5341 gtgttggtga ggccgcggat gtggccgctg acgacggaca cggcgccgaa ttcgcccatc
     5401 gcgcgcgcgt tgcataaaag cacgccgtag aacaaggccc atttgatgtt gggaagcgtc
     5461 acgcgcagga aggtctggaa gcccgacgcg ccgagtgtgg cggcggcttc ctcttcctgg
     5521 cggccctggt cggtcatcag cggaatcagt tcgcgtgcga cgaacgggaa ggtcacgaat
     5581 atcgtcgcca cgacgatgcc gggcagcgcg aagatgacct tgatgccatg gtcgcgcagc
     5641 tccgggccga accagccctg aagcccgaaa atcagaacat agaccaatcc ggagaccacg
     5701 ggcgaaacgg aaaacggcag atcgatcagc gtcgtcagga accctttgcc gcggaaatcg
     5761 aatttggcaa tggcccagga ggcggcaatg ccgaacaccg cgttcaacgg taccgcgatg
     5821 gccgccacca gcaaagtcag ccgtatggcg gcgagcgcgt ccggttccgt gatggctgcg
     5881 agcgcgaagt caagaccctt cgacagcgcc tgcgtgaaca ccatggccag cggcaggacg
     5941 aggaacaggc cgatgaagcc gatcgcaacg gccgaaagca ccacgcgcat cagcggatgg
     6001 tcctgtgtcg cttttctgcg gtggacctcg ctcattggcg cgatgcagcc cagttctgca
     6061 ggagattcag cgtcaggatg aggatgaacg acgccgccag catgatcacg ccgatcgccg
     6121 acgcaccggc gtagtcgaac tgttccagct tgatgatgat gagcagcggc gcgatctctg
     6181 aaattgcggg caggttgccg gcgatgaaga tcacggaacc gtattcgccg accgcgcgcg
     6241 cgaaggccag cgtcgtgccg gtcaccagcg agggcaggag cgtcggcagg accacgcgcc
     6301 agattgcctg cagccgcgtg gcgccaagcg tcgccgccgc ctcttcccct tccaaactca
     6361 gatttgccag caccggctgc agcgtgcgca cgacgaaggg caggccgatg aaggtcaagg
     6421 cgacgatgac gcccagcggc gtgtaagcga ctttgacacc gagatggctg agcggctcgc
     6481 cgagccagcc ttgcggcgcg tacagcgtcg acagcgcgat gccggccaca gccgtcggca
     6541 aggcgaaggg aagatcgacc agtgcgtcca ggagtctttt acccggaaag cgatagcgca
     6601 ccagtaccca cgccacgaca aaaccgaaaa ccgcattgat gcctgccgca atggccgctc
     6661 ccccgaacga aaggcgcagc gcggccagca cgcgacggtc ggtcaggatc tcccataccc
     6721 cgtcgaagcc caaagaccaa gggcggatga ccagcgccgc caacgggatc aggacgatca
     6781 ccgacagata gaacagcgcg aacccgagcg acaaccccaa accgggtagc acgctcgggt
     6841 ttcgccagtg taaaggggaa gaagctgccg ctgtgttcat tttccgggtt tataaatctg
     6901 atcgaacacg ccgccgtccg agaaatgaat ctgctgcgcc ttttgccatc cgccgaagac
     6961 gtcatcaatg gtaaagagcg ggatcttcgg gaaatcggcc gcatgcgccg ccaaaatcgc
     7021 cggatcgcgc ggacgataga aattcctcgc ttcgatctcc tgtgcctcct tgctgtagag
     7081 gaattgcaga taggcttcgg ccactttgcg ggtaccgtgg cggtctacgt ttttgtcgac
     7141 cagggcaacc gggggctcgg ccaggatggt attggacgga tagacgatct ggaacttgtc
     7201 gctgccgtat tccttca
//
LOCUS       DASWPU010000604         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_48228,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000604 DASWPU010000000
VERSION     DASWPU010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48228"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VGC27_12860"
     CDS             complement(<1..906)
                     /locus_tag="VGC27_12860"
                     /inference="COORDINATES: protein motif:HMM:NF025174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283501.1"
                     /translation="MTMRCDTRRLALALAAIGLMAGGCSESLWGKKEAPASAAQTWQK
                     TDSGVIVSPTAFAGRKVRLVVMTPSIIRVTVAPAGNFDLPESLVVTAKPDPAKFEVAA
                     DDGAVTLKTDAASAKVSLETGAVTLFDAAGKEILTPATAGDAVPIKVGGQDFYAIRQE
                     FNRGSDEGFYGLGQHQNGQMNYNGEDVILAQHNMDVAVPFLVSTRNYGILWDNYSITR
                     FGWPKEYTTLSAELKLRDANGKEGGLTARYTDKGKLLAERVENDPNYQFIEDSVNFPP
                     EVANPKKIKIANGNPVPVSPTLTVTW"
     gene            complement(923..>2221)
                     /locus_tag="VGC27_12865"
     CDS             complement(923..>2221)
                     /locus_tag="VGC27_12865"
                     /inference="COORDINATES: protein motif:HMM:NF037199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HEY0283502.1"
                     /translation="HDKRLVLLWFGTWKNTAPGYTPAWVKRDTVRFPRMVDEKGETIY
                     ALSPHAKTTLAADRRAFVRLMEFLKENDKQRTVILMQVENEAGTYKLVRDHSPAAEAL
                     FNAQVPVKLIAGLEKQSGTWKEVFGKDADEFFHAWSIARFVEDVAAAGKQAYPLPMFV
                     NAALRDPDKDQDPKTYAAGGPTWNVLDVWKIAAPSIFTASPDVYGRTYDDVIGQIRRY
                     TRPDNPLMIVEIGSAAKFARFFYPVLGNHGIGFAPFGFDDTGYSNYPLGAKKIDDETV
                     APFAAHFAQVAPMMRDWAKIAFENETFGVAEPDDHQAQTIALGRWTATVTYGEWQFGE
                     RSWFPDADKPDWADKPTGGMTIAKLGPDEFLVFGYRARIGFALTDPAAGKKAMYERVE
                     EGHFTDGKWVADRRWNGDLTDYGLNFTDLPQVLRVKMMTY"
BASE COUNT          375 a          710 c          701 g          435 t
ORIGIN      
        1 ccaggtgacc gtcagcgtcg gactgaccgg caccggattg ccgttggcga tcttgatctt
       61 cttgggattg gcgacctccg gcgggaagtt caccgaatcc tcgatgaact ggtaattggg
      121 atcgttctcc acccgctcgg caaggagctt gcctttgtcg gtatagcgcg cggtcagccc
      181 gccttccttg ccgttggcat cgcgcagctt aagctcggcc gacagcgtcg tatattcctt
      241 cggccagccg aagcgggtga tggaataatt gtcccacaat atgccgtagt tcctggtcga
      301 aaccaggaac ggcaccgcca cgtccatatt gtgctgggcg aggatcacat cctcgccatt
      361 gtagttcatc tggccgttct gatgctggcc gaggccgtag aagccttcat cgctgccgcg
      421 gttgaattcc tggcggatgg catagaaatc ctgccccccc accttgatcg gcacggcatc
      481 gcccgccgtg gccggggtca gaatttcctt gcccgccgca tcgaacagcg tgaccgcccc
      541 ggtttcgagc gagaccttag cactggccgc atcggtcttc agcgtgaccg cgccatcatc
      601 cgccgctacc tcgaatttcg ccgggtcggg cttggccgtc accaccagac tttccggcag
      661 atcgaaattg ccggccggcg ccaccgtgac gcggatgatc gacggcgtca tcaccacaag
      721 gcgcaccttg cgcccggcga aggccgtggg ggatacgatc acgccgctat cggtcttctg
      781 ccaggtttgc gccgcggagg ccggcgcttc cttctttccc cacagggatt cggagcagcc
      841 gcccgccatc aggccgattg ccgcaagcgc cagcgcaagg cggcgcgtgt cacagcgcat
      901 ggtcatcgaa acactcccgg tctcaatagg tcatcatctt gacccgcagc acctgcggca
      961 ggtcggtgaa gttcaggccg taatcggtca gatcgccgtt ccagcggcga tcggcgaccc
     1021 atttgccgtc ggtgaaatgg ccttcctcga cgcgctcata catcgccttc ttgcccgccg
     1081 ccggatcggt caaggcaaag ccgatgcggg cgcgatagcc gaagaccagg aactcgtccg
     1141 gtccgagttt ggcgatggtc atgccgcccg tgggcttgtc ggcccagtcc ggcttgtcgg
     1201 cgtccgggaa ccaggaacgc tcaccgaact gccattcgcc ataggtgacg gtggcggtcc
     1261 agcggccgag cgcgatggtc tgcgcctggt gatcgtccgg ttcggccacg ccgaaggttt
     1321 cgttctcgaa ggcgatcttc gcccagtcgc gcatcatcgg cgccacctgc gcgaaatgcg
     1381 cggcgaacgg cgcgacggtt tcgtcgtcga tctttttggc gccgagcgga tagttggaat
     1441 agccggtgtc gtcgaagccg aagggcgcaa agccgatgcc gtgattgccg agcaccggat
     1501 aaaagaagcg ggcgaatttc gccgccgagc cgatctcgac gatcatcagc ggattgtcgg
     1561 ggcgcgtata gcggcggatc tggccgatga cgtcgtcgta agtccggcca tagacgtcgg
     1621 gcgacgcggt gaagatgctg ggcgcggcga tcttccagac gtcgagcacg ttccaggtcg
     1681 gcccgcccgc cgcataggtt ttgggatcct ggtccttgtc gggatcgcgc aaagcggcat
     1741 tgacgaacat cggcaacgga taagcctgct tgcctgccgc cgccacgtcc tcgacgaagc
     1801 gggcgatcga ccaggcgtgg aagaactcgt cggcatcctt gccgaacacc tctttccagg
     1861 tgccggactg cttctcgagg ccggcgatca gtttgacggg gacttgcgcg ttgaacagcg
     1921 cctcggccgc aggggaatga tcgcgtacca gcttgtaggt gccggcctcg ttctccacct
     1981 gcatcaggat gacggtgcgc tgtttgtcgt tctccttgag gaattccatc agccgcacga
     2041 aggcgcgccg gtcggccgcc agcgtcgtct tggcgtgggg cgagagcgca tagatcgtct
     2101 cgcccttctc gtcgaccatg cgcgggaagc gcaccgtatc gcgcttcacc caggcaggcg
     2161 tatagccggg cgcggtgttc ttccaggtgc cgaaccacag cagcaccagc cgtttgtcgt
     2221 g
//
LOCUS       DASWPU010000605         5624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_97431,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000605 DASWPU010000000
VERSION     DASWPU010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97431"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="VGC27_12870"
     CDS             complement(<1..1373)
                     /locus_tag="VGC27_12870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0283503.1"
                     /translation="MEASREPLPWRLVRSFSLKLLLLAVILLAVPLILYWQFQRAERQ
                     EFALLQNAAQRSGRIIAAMLRPRFAGFRHEIHGELREALAGAAIDNTKVKVLVRPEGA
                     DKDDFIYIASAPPLPAEYLKQETRDLVRSGIFQRLAPTCDKAAELDVRFVNPAGKQEV
                     ITSMTPVHIDRNCWIVVTAQSTAMLTPSPLNNSFWTIPAMQAAAAIYVLSAALVIWLF
                     LHLWRNVARFRAAAHQIRMHGAGAASFRERNTIPELARVTEDFDSLVAALTASQAFIK
                     QAAEENAHALKAPLGVIAQALEPLKRAAAPSDTTAQRSLQLMERSIARLDTLVSAMRD
                     VEQAAAEAVYPMRRLVNLSRFLAQLLGGYEATLAAQGKHLAVSLEKHVSALANEDVLE
                     PVIENILENAAGFTAQGGTIEVTLERHGALARIVVADRGPGIEPARLVHIFDRYVSYR
                     SDAATDVA"
     gene            complement(1357..2112)
                     /locus_tag="VGC27_12875"
     CDS             complement(1357..2112)
                     /locus_tag="VGC27_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0283504.1"
                     /translation="MEQASPRPHRGHVLVVDDDDLFRESVGTNLADAGFSIDAFADGA
                     SAIRRLKSGPAADLVLLDWKMPGMTGIEVLQRLRGESIEIPVIFLTVLNDQIYEEAGL
                     LGGAVDFIEKSRSFSILLKRIELILTGHKSRMQRPAADGKGIVQIGELTLRFDCRRAS
                     WRQADAGLTLTEFNIVACLTELAGRDVSYREIYDIVHGEGFAAGDGEIGYRTNVRAFI
                     KRIRQKFRNLDPNFRCLENFPGFGYRWRDGSEP"
     gene            complement(2099..2575)
                     /locus_tag="VGC27_12880"
     CDS             complement(2099..2575)
                     /locus_tag="VGC27_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283505.1"
                     /translation="MVAAALIGMALCGCAAIDKATGWSSPDTGQSPAPPQPVAAPPPA
                     APAAPKAKPVGRGTPEQRRPAKLAAINPNSLIGLNPPAVEKLLGPPATVSKDDPSLVW
                     TYAAGDCSFRIFFYPDLKTSSFRALKYGGTDGKDSQIKTSDACIRNILTVKNNGAG"
     gene            3066..3416
                     /locus_tag="VGC27_12885"
     CDS             3066..3416
                     /locus_tag="VGC27_12885"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY0283506.1"
                     /translation="MKKFLMVCAVSALSAGLFFPINAQDAAQPVTSPSGQTATQPDLV
                     GQTVFNSEGSTIGTVSSVATDAQGQQAAVVDISGKKILFPVSSFTPKDGGGYTTTLSA
                     EEIKNLPEAGGSAN"
     gene            complement(3556..3882)
                     /locus_tag="VGC27_12890"
     CDS             complement(3556..3882)
                     /locus_tag="VGC27_12890"
                     /inference="COORDINATES: protein motif:HMM:NF018993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEY0283507.1"
                     /translation="MGDAFEERKKKHEDKWAHDEELRFKIGARRNKLLGLWAAAELGL
                     TGVEVEEYAKSVVVADLEGAGAVFRKLRGDFDAGGLGHSDHVIQRQMAEFLAAATDQL
                     MHESKG"
     gene            4089..4853
                     /gene="purC"
                     /locus_tag="VGC27_12895"
     CDS             4089..4853
                     /gene="purC"
                     /locus_tag="VGC27_12895"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY0283508.1"
                     /translation="MKRRRMIYEGKAKILYEGPEPGTVIQYFKDDTTALDTQKTAVIE
                     GKGVLNNRISEYLMTQLNAIGVPTHFVRRLNMREQLIKEVEIIPLVVVVRNVAAGSLS
                     KRLGIEEGTALPRSIIEYFYKNDALHDPWVSEEHITAFGWASPQDLDDMVNLTIRIND
                     FLSGLFLGVGIKLVDFKLEFGRLWENDYMRIVLADEISPDSCRLCDAATNEKMDKDRF
                     RRDMGGLMEAYSEVARRLGIMPESGQGEAKGPMLVK"
     gene            4944..5186
                     /gene="purS"
                     /locus_tag="VGC27_12900"
     CDS             4944..5186
                     /gene="purS"
                     /locus_tag="VGC27_12900"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY0283509.1"
                     /translation="MKARVFVTLKNGVLDPQGKAIGHALNGLGFQGIGEVRQGKVIDI
                     ELTERDEAKARASLTEMCEKLLANTIIEKYEIELKE"
     gene            5311..>5624
                     /locus_tag="VGC27_12905"
     CDS             5311..>5624
                     /locus_tag="VGC27_12905"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY0283510.1"
                     /translation="MKSAVVIFPASNCDRDAAVALEQMTGIKPHMVWHADSEVPEVDL
                     IVLPGGFCYGDYLRCGAMASQSRVMRDVKAKADKGVAVLGICNGFQALTETRMLPGAL
                     MR"
BASE COUNT         1128 a         1697 c         1754 g         1045 t
ORIGIN      
        1 gcaacgtctg ttgccgcatc gctgcgatac gatacgtaac ggtcgaagat atgcaccagc
       61 ctcgcaggct cgatgcccgg gccgcgatcg gccaccacga tgcgcgccag ggcgccgtgc
      121 cgttcgagag tcacttcgat cgttccgcct tgcgccgtga agccggcggc gttttcgagg
      181 atgttctcga tcacgggctc caggacgtct tcgttggcga gcgcgctgac atgcttttca
      241 agcgacaccg ccagatgttt gccctgcgcg gccagcgtcg cctcgtagcc gccgagcagc
      301 tgggcgagga aacgcgagag atttacgagg cggcgcatgg gataaacggc ctccgcggcg
      361 gcctgttcca cgtcgcgcat ggccgagacc agcgtatcca ggcgggcaat ggaccgctcc
      421 ataagctgca ggctgcgctg cgccgtcgta tcggatgggg ccgccgcgcg cttcagaggc
      481 tcaagagcct gcgcgatgac gcctaaaggc gccttgagag catgcgcatt ctcctccgcg
      541 gcctgcttga tgaacgcttg cgacgccgtc agcgcggcca cgagggaatc gaaatcctcg
      601 gtcacgcgcg ccagctcggg gatcgtgttc cgctcgcgga aggacgccgc gccggcgccg
      661 tgcatgcgaa tctggtgtgc cgccgcacga aacctcgcaa cattgcgcca caggtgaagg
      721 aacagccaga tgacaagcgc cgcgctgagc acataaatcg ccgccgccgc ctgcatggcc
      781 gggatcgtcc agaacgagtt attgagcggc gacggcgtca acattgccgt actctgcgcc
      841 gtgacgacga tccagcagtt cctgtcgata tggacgggcg tcatcgacgt gatgacctcc
      901 tgcttgccgg cgggatttac aaagcggaca tcgagttcgg cggctttgtc gcaggtcggc
      961 gccaggcgct ggaagattcc ggaacggaca aggtcgcggg tttcctgttt caggtattcg
     1021 gcgggcaagg gcggcgccga ggcgatgtag atgaaatcgt ccttgtcggc gccttccggg
     1081 cgcaccagaa ccttcacctt cgtgttgtcg atcgccgcgc ccgccaacgc ttcgcgaagc
     1141 tcgccgtgta tctcatggcg gaacccggcg aaacgggggc gcagcatggc ggcgatgatg
     1201 cgcccgctgc gctgggcggc attttgcaga agggcgaatt cctggcgctc cgcacgctgg
     1261 aactgccaat agaggatcag cgggacggca aggagaatga cggccagaag aagcagcttc
     1321 agggagaacg aacggaccag acgccacggc aacggctcac ggctcgcttc catcgcgcca
     1381 cctgtagccg aatccgggga agttctcgag acaacggaag ttgggatcga ggttcctgaa
     1441 tttctgccgt atccgcttta tgaacgcgcg cacgttggtg cggtagccga tctcgccgtc
     1501 gccggccgcg aagccttcgc catgcacgat gtcgtagatt tcgcgataac tgacgtcgcg
     1561 gccggccaat tccgtcagac aggcgacgat gttgaactcg gtaagcgtca ggccggcgtc
     1621 ggcctgcctc caggaggcgc ggcggcaatc gaagcgcaag gtcagctctc cgatttgcac
     1681 aattcccttg ccgtccgcgg ccgggcgttg catccgcgac ttatgccccg tcagaatcaa
     1741 ttctatccgc ttgagcagaa tggagaagct tctcgacttc tcgatgaaat ccaccgctcc
     1801 gccaagcaga ccggcttcct catagatctg atcgttgagg acggtcagaa agataaccgg
     1861 gatttcgatg ctttcgccgc gcagccgttg cagcacctcg attcccgtca tgccgggcat
     1921 tttccagtcg agcagtacga ggtccgcggc aggtccgctt ttaaggcggc ggatggcgga
     1981 cgctccgtcg gcaaaggcgt ctatcgaaaa gccggcgtcc gcgagattcg tgcccacgga
     2041 ttcgcggaac aagtcgtcgt catcgacgac gagaacatgg ccgcggtgcg gacgcggact
     2101 agcctgctcc attgttcttc accgtcaata tgttccggat acaggcatcc gatgttttga
     2161 tctggctgtc cttgccgtcg gttccgccat atttgagggc gcgaaatgaa gacgtcttga
     2221 ggtcgggata gaagaatatg cggaacgaac agtccccggc agcataggtc cacaccagcg
     2281 aagggtcgtc cttgctgaca gttgccggcg gccccagcag cttttcgacg gcagggggat
     2341 tgagccctat gagactgttg ggattgatgg ccgcgagttt tgccggcctt ctttgctccg
     2401 gcgttccgcg accgacgggt tttgctttcg gtgcagcagg cgcagccgga ggcggagccg
     2461 cgaccggctg cggcggcgcg gggctctgcc cggtgtcggg agaggaccag cctgttgcct
     2521 tgtcgattgc ggcgcagccg cacagcgcca taccgatgag ggccgcagcg accatgggcc
     2581 gccagtctgc ggtccatttg ccgttcgcgc ggcaaggcgt tgtatcgcgt tgggcgcaaa
     2641 aggcctgcac ggcgcgcgaa gcgagcctga cggtcgtttt gtttcggagc aggcgccgcc
     2701 ccggatatgc agagtcacgt ctcttcatcg ctttgccgga ttcaaggcca gggcgggtgc
     2761 atccggcaac atgccggaga gcgccatgcg gtcacagggt tcaagaacgt cgccgaggcg
     2821 catcgggcag atcgtcaagc gaaccgtcgc gatagcgtgg ataaaagccg ccaccccctg
     2881 catgaaacac ctcgcgcagc ttcggacaaa cacgcgcaat ctcttttttg tcccacataa
     2941 gtcacaaccg tacaagcagg ttcggccata caagcagttt gtcgttggct tcactctggc
     3001 gcgaatacgc gcgccggtgc gggggagctg taacgcccga aagggccaga attgaagagg
     3061 acgagatgaa aaagtttctg atggtatgcg cggtttccgc cttgagtgcc ggcctttttt
     3121 tccccatcaa tgcccaggac gcggcgcaac cggtgacatc cccatccggc caaaccgcga
     3181 cgcaacccga cctcgtcggc caaacggtct tcaactccga aggcagcacg atcggcacgg
     3241 tgtcgtccgt cgcgacagac gcccaaggcc agcaagccgc cgtcgtggat ataagcggca
     3301 aaaagattct ctttccggtc agttcgttca cgccgaaaga cggcggcggt tacacgacca
     3361 cattgagcgc cgaagagatc aagaatctgc ctgaagcggg cggcagcgcg aactgacaat
     3421 ggcgcagcgg ccgtttttgc aaaaagaaac accgcaatca cgaacggtct tcgccttgga
     3481 aaaaattaag cggcgcgcgt aaaagacggt tttgcgcgcg ccgttttcgc attgcgcggc
     3541 acagcgtata acgaactagc ccttgctctc gtgcatgagc tggtcggtgg cggcggcgag
     3601 aaactcggcc atctgccgct ggatgacgtg atcggaatgg ccgagcccgc ccgcgtcgaa
     3661 atcgccgcgg agcttgcgaa acacggcgcc ggcaccctcg agatcggcca cgacgacgga
     3721 tttggcgtat tcctcgacct ctacccccgt cagccccagt tcagcggccg cccatagtcc
     3781 caacagcttg ttgcggcgcg cgccgatctt gaagcgcagt tcctcgtcat gagcccattt
     3841 gtcctcatgt ttctttttgc gctcctcgaa cgcgtcaccc atagacaacc tcttgaagaa
     3901 tattgggaaa tctcgccaaa caggctccat tgcccggtac aataccgcac cggggcggca
     3961 actgcgacgg ggcctggcgg gtgtgcgtta cgggcacgtt gccgcgcggg cctggcgccg
     4021 ctattttgcg acctcggcgg ggactcaaaa gtcgaggccc cgtttcgcgt cttaggcgga
     4081 gaagaaacat gaagcgccgg cgcatgatct acgagggcaa ggccaaaatc ctctatgagg
     4141 ggccggagcc cggaacggtc atccagtatt tcaaggacga caccaccgcc ctagacaccc
     4201 agaaaacggc cgtcatcgaa ggcaaggggg tgctcaacaa ccgcatcagc gaatatctga
     4261 tgacgcagtt aaacgcgatc ggcgtgccca cgcatttcgt gcgccgcctc aacatgcgcg
     4321 agcaattgat caaggaggtg gagattattc cccttgtggt tgtggtgcgc aacgtggccg
     4381 cgggatcgct gtccaagcgg ctgggcatcg aggaaggcac cgcgctgccg cgctccatca
     4441 tcgaatattt ctacaagaac gacgcgctgc acgatccctg ggtgagcgag gaacacatca
     4501 ccgccttcgg ctgggccagc ccccaggacc tcgacgacat ggtgaacctg accatccgca
     4561 tcaacgattt tctctccggc ctgttcctcg gcgtcggcat caagttggtc gatttcaagc
     4621 tcgaattcgg gcggctgtgg gaaaatgact atatgcgcat cgttctggcc gacgagatca
     4681 gtcccgattc ctgccggctg tgtgacgcgg ccaccaacga gaagatggac aaggaccgct
     4741 tccgccgcga catgggcggc ctgatggaag cctattccga agttgcgcgg cgcttgggaa
     4801 tcatgcccga atcggggcaa ggagaagcca aggggccgat gttggtgaag tgagtccctc
     4861 catgcttcga caagctcagc atgaaggtac tgctatcgcc ctcatcctga gcccgtcgaa
     4921 ggatgaaggt ctggattgaa cacatgaaag cgcgcgtatt tgtgacattg aagaatgggg
     4981 ttctcgatcc gcaaggaaaa gccatcggcc acgcgctgaa cgggctgggc tttcaaggca
     5041 tcggcgaggt ccgccaaggc aaggtcatcg acatcgaatt gaccgagcgc gacgaagcga
     5101 aagcccgcgc cagcctgacc gagatgtgcg agaagctcct cgctaatacg attattgaaa
     5161 agtatgagat cgagctaaag gaatgaacct tgttctttcg tcatggccgg gcttgacccg
     5221 gccatccagg acaaaaagca aactgcattt cgtcgtgacg aatggatggc cggctcgcgc
     5281 ttcgctggcc ggccatgacg aggtgatgtc gtgaaatccg ccgtcgtcat ttttcccgct
     5341 tccaattgcg accgcgacgc ggccgtggcc ctggagcaga tgaccggcat taagccgcac
     5401 atggtgtggc acgccgacag cgaagtgccc gaggtcgatc taatcgtcct gcccggcggc
     5461 ttttgctacg gcgattatct gcgctgcggg gcgatggcct cccaaagccg cgtcatgcgc
     5521 gatgtgaagg ccaaggcgga caaaggcgtg gcggtgctcg gcatctgcaa tggcttccag
     5581 gcgctgaccg agacccgcat gctgcccggc gccttgatgc gcaa
//
LOCUS       DASWPU010000606         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_102854,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000606 DASWPU010000000
VERSION     DASWPU010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102854"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..979)
                     /locus_tag="VGC27_12910"
     CDS             complement(join(219..640,640..979))
                     /locus_tag="VGC27_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611229.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0283511.1"
                     /translation="MDCDALRDDQWERIRGYVPGGTKGKRGPRTDNRRFLNALLWMAR
                     SGGRWRDLPKRLGNYQSVKRRYYRWIEMGVLDDMLAVLSREADLEWLMIDSTIVRAHQ
                     HAAGARKEKGGPDAQGLGRSRGGLSTKIHAAGDALGNPLRLIGTPGQRNDIAFAHELV
                     DGFNAGATIGDKGYDADHLCNEIAETGAEVVIPPKRNRKIQRAYDADLYEERNIIERF
                     FNKLKQFRRVATRYDKLLVNFMGFVKLAAIAIWLK"
     gene            complement(1214..>1889)
                     /locus_tag="VGC27_12915"
     CDS             complement(1214..>1889)
                     /locus_tag="VGC27_12915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0283512.1"
                     /translation="IVVFLGDYIDRGPASKEVLDLLLKCERTREAIFLKGNHETFVHR
                     FLSEPAVLDEWRLCGGLETLVSYGLKPSINPDASEQMQLAEELARVIPKRHLEFLESF
                     DLSFSCGDFLFVHAGIRPGVPIRKQSEEDLLWIREEFLSCEERFEKFVVHGHTPVPTI
                     DIRSNRINIDTGAFATGRLTCIVIEGTSIVPLIDVRDWITGTADIQSVQTGQTRIEPV
                     AQTLAV"
BASE COUNT          383 a          594 c          527 g          385 t
ORIGIN      
        1 agttgcggcc cagggtcggc attccgcatc cgccggcagc gccgccagcc cccaacccgc
       61 ctgtcgtgag ccagcaggtt gttccgctgc caccgccgat cgagattagt ccggctcctg
      121 gcgccgtgcg ccagccgagg ccacggccgc cgcttgtgct gacgccgccg attccaggtc
      181 cgcagcgacc ggcgttctag ggagcggtga ctaattaact atttgagcca gatagcgata
      241 gcggcaagtt tgacgaagcc catgaagttg acaagcaact tgtcatatcg cgtcgcgacc
      301 cggcggaact gtttgagctt attgaagaag cgctcgatga tgttgcgctc ttcatagaga
      361 tcggcgtcgt aggcgcgctg gatcttgcgg ttccgcttgg gcgggatgac gacctcggct
      421 ccggtttcgg cgatctcgtt gcacagatga tcggcgtcgt agcccttgtc gccgatcgtg
      481 gcgcccgcat tgaagccatc gacgagttca tgtgcgaagg cgatgtcgtt gcgctgcccc
      541 ggcgtaccga tcaggcggag cggattgccc agcgcgtcac cggcggcgtg gatcttggtg
      601 ctcagaccgc cgcgagaccg gcccaggccc tgggcatccg cccccctttt tccttgcgcg
      661 cgccagcggc atgctgatgc gcccgcacga tggtcgaatc gatcatcagc cactccagat
      721 cggcttcgcg cgaaagaacc gccagcatat cgtcgagaac gcccatctca atccagcgat
      781 agtaacgtcg cttcaccgat tgatagttgc ccagccgctt cggcagatca cgccagcgcc
      841 cgccagaacg cgccatccac aacagcgcat tcagaaaccg acggttgtcg gtgcgaggcc
      901 cgcgcttacc cttggtcccg ccaggcacat agcctcgaat tcgttcccac tgatcatccc
      961 gaagagcgtc gcaatccatc gcagatcccc aaaaatcagc gttgaatctg atttgccctc
     1021 tcttgggaat cccaaatcct taaatcgtca ccacgcccta aggcttgcga agccgaacgt
     1081 caggtcctcc accattttct cgatgccggc ctttgttggg gcgcgcgcta tcgcgatcat
     1141 ggcatcctcg gacactcgga agccgcccat tggctcccgc cggaagatca cgccacgagg
     1201 cgcctgtgac attctagact gccagggtct gcgcaacagg ctcaatcctc gtttgccccg
     1261 tctgtacaga ctggatgtcc gccgtgccgg ttatccagtc gcgtacgtcg atgagcggca
     1321 cgattgaggt gccctcgatc acgatgcaag tcaggcgtcc ggtcgcgaag gctccggtgt
     1381 caatattaat ccgatttgac cggatatcga tcgtcggaac cggggtatgg ccgtgcacga
     1441 caaacttctc gaatcgctct tcacaggaga gaaactcctc acgaatccaa agtaaatctt
     1501 cctcgctctg cttccgaatg ggaacgccgg ggcgaatacc ggcatggaca aacagaaaat
     1561 ccccgcaact aaacgacaga tcgaaagatt caagaaattc caggtgccgt ttgggaatga
     1621 ccctggccag ttcttcggcc agttgcattt gttcggaggc gtcgggattg atcgatggct
     1681 tgagtccgta ggacacgagg gtttccagac cgccgcatag ccgccattca tccaggactg
     1741 ccggctcgct cagaaagcga tggacaaagg tctcatggtt gcctttgaga aagatcgcct
     1801 ctcttgtccg ctcgcattta agcagaagat ccagcacttc ttttgaggcc ggtccccggt
     1861 cgatatagtc gccaagaaaa accacgatc
//
LOCUS       DASWPU010000607         6091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_60497,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000607 DASWPU010000000
VERSION     DASWPU010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60497"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VGC27_12920"
     CDS             <1..1071
                     /locus_tag="VGC27_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0283513.1"
                     /translation="NVTDCPAVPNSDRAAACTFVGSDNDGTVTYPGVSNHYTNVLPSF
                     NFRAGLTDTLQLRLAYSQGLVRPDLERMRNYTQLSYSFGSMQQHDSFAAVNALRGQGS
                     NPYLKPTYAQNYDASIEWYFAPTGNVALALFYKTIANYIMSGVAPMSFTRNGVTQIFN
                     VGSYVNGSKGTVEGFEFAYTQFFDSLPGAWGGLGVQANYTKLYNTGGANPVAAIYNPI
                     NGVNVYQGINGTDFAPQALGYAGDTRLPMEGMSNDSFNLALMYEKYGISSRVAYNWRS
                     RNLVNSYPANIFQPVFQRSYGQLDASLLYTFLDHYKIGVQAANLLKQTAVLEVGQTVS
                     TKHEYQWVEGERKFSVVLRASW"
     gene            1200..3077
                     /locus_tag="VGC27_12925"
     CDS             1200..3077
                     /locus_tag="VGC27_12925"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY0283514.1"
                     /translation="MNSETGTENSTVSYEAALALHMRGRVGEAEHMYEAFLKSHPDHS
                     EAWHGLGIIRLRSGRTDTAIDNLRKAVAAGGSAVVKNNLGVALCAAQRFAEAADVYRA
                     VVQSDPDAVSSLGNLGQVLNQLRAFAEAVEVLQKGLRLAPDNARLHNQLAIALAECRR
                     LDEAQAHFEKAVALDPGRSEFHCDLGALLLKRDRIRQAAECYRRALSVTPASPMALCG
                     LGEALGGLNRHEEAVACFRQAVALAPGYAAAHYNCGTALTYLGRMAEAETAFVRAAEL
                     EPDNPAYRGALIAMKKTTASSAHLKALQALAIDADRLGEREAMELQFTLAKAYDDIGD
                     HARAFEALRLGNAAKRRQNPYELQQDLDRFRAIADAFTAELLAAHAGQGDPTEVPVFV
                     VGMPRSGTTLVEQILASHPDVFGAGELTILPDLISSGRAGGDFPAAVATLGAEAWRAI
                     GETYAGTLRAQAAEAVRITDKLPLNFQLVGLIRLALPKARIIHVMRDPLDTCFSCYFT
                     LFAHELGFSDDLADLGRYYRGYQSLMAHWRSVLLEGAMLEIQYENLVADLETEARRLI
                     GYCGLPWDARCLDFHNTVRPVETASMLQVRRPLFQTSVGRAGPYRPWLAPLERALAGE
                     T"
     gene            3121..4569
                     /locus_tag="VGC27_12930"
     CDS             3121..4569
                     /locus_tag="VGC27_12930"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY0283515.1"
                     /translation="MVSRLLAEGIHLCRTGRFAQAKAIYDRIMMLAPQRVEPYLNCGQ
                     IGLAEGQYDDALSWFEKVRRLKPDLPPALEGAGAALLALGRPAEALAIFERLQTLDAK
                     SSAALHGAGEALKQLGRLKEARAVLERAIELAPDVAGHHYLLAQLARFVENDPRLAGL
                     ERLRNKAASLPEPDRCQLHFALGKAYDELGRHAQAFEHWRAANAIKRRHIAYDESMFL
                     GILRDLAAAFTPEVMAARYGAGDPSRLPVFVVGMPRSGTTLVAQIIASHPDAFNVGES
                     MYLHYLLGQNRAGPDFPAHFTSVPKEGLRNMGAFYVAHLNASAPKASRIVDKLTANFM
                     LCGLIRIILPNARIIHVRRDPLDTCLSCYANLFSQNIDYSYDLGELGRYYRAYETLMA
                     HWRRILPEGAMLEVSYEELVSDFPAQARRIVGYCGLGWDEACLSFHLTKRVVHTLSAV
                     QVRQPVYGSSVGRWKAYASHLGPLIEALGAAR"
     gene            4711..5244
                     /locus_tag="VGC27_12935"
     CDS             4711..5244
                     /locus_tag="VGC27_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283516.1"
                     /translation="MRIFAAIAACSCLFLLSAPAFGETFTSEEYGFTAEFPARPTVGP
                     PQGSETDSKGNFISTSVMIKTQVLGVYTAMVTVDSYLVPLKIDPSSTLLAMSRSFVAQ
                     LDATTTSSKPGRLDGFRARFFSYDTADHSASGKGIVVIVPAKKPRTYLVLTMHTSAAS
                     ADEIAALDKFLDSFHVK"
     gene            5639..>6091
                     /locus_tag="VGC27_12940"
     CDS             5639..>6091
                     /locus_tag="VGC27_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0283517.1"
                     /translation="MTDKSDAGPIVSLRNVVKTYSDDGLKVSAVNDISFDIQRQRFTM
                     IVGPSGSGKTTLLNLIGCIDVPTAGTIEVCGEKVAKLSDNKITDFRARNIAFVFQNFN
                     LYPVLSAYENVEYPLLLIGMPAKQRKERTLAMLESVGLADQRRHRPNQL"
BASE COUNT         1128 a         1929 c         1845 g         1189 t
ORIGIN      
        1 aatgtcactg attgcccggc cgtccccaac agcgaccgcg ccgcggcttg tacattcgtc
       61 ggcagcgaca acgacgggac cgtcacctat ccgggcgtca gcaaccacta caccaacgtg
      121 ctgccgtcgt tcaatttccg cgccggtttg accgacacgc tgcagctccg tttggcctac
      181 tcgcaaggtc tggtcaggcc agacctcgaa aggatgcgca actacaccca gctttcctat
      241 tcgtttggct cgatgcagca acatgactcg tttgcggccg tcaacgcgct gcggggccag
      301 ggcagcaatc cgtacctcaa gcccacctac gcgcagaact acgacgcgag cattgagtgg
      361 tactttgcac cgaccggcaa cgtggcgctg gctctgtttt ataagaccat cgccaactac
      421 atcatgagcg gcgttgcgcc catgagcttc acgcgcaatg gcgtgaccca gatcttcaac
      481 gtcggcagct acgtcaacgg ctcgaagggc acggtggaag gcttcgaatt cgcctacacc
      541 cagttcttcg attcgttgcc gggtgcttgg ggcggtctgg gcgtgcaggc caactacacc
      601 aagctctaca acaccggcgg tgccaatccc gtcgccgcga tctacaaccc gatcaacggc
      661 gtgaatgtct atcaaggcat caacggcacc gatttcgcgc cgcaggccct tggttacgcc
      721 ggcgacacgc gacttccgat ggaaggcatg tcgaacgaca gcttcaacct ggcgctgatg
      781 tacgaaaagt acgggatttc gtcccgcgtc gcctacaact ggcgttcgcg caacttggtc
      841 aactcctacc cggccaacat cttccagccg gtgttccaga ggagttacgg ccagctcgac
      901 gcctcgcttc tgtacacgtt cttggaccac tacaagatcg gcgtgcaggc cgccaacctg
      961 ctgaagcaga ccgcggtcct ggaagtcggc cagacggtat ccaccaaaca cgaataccag
     1021 tgggtcgagg gcgaacggaa gttctccgtg gtcctgcgcg caagctggta atcgcgcaaa
     1081 gtctaggtgc aacgaaaccc cccggcggcg tgccgccggg gggttttctt tgtcgaaaac
     1141 tacttcgcac aaaccggccg gattggatat tctctccgtg tcgcgcattc ggatgatcga
     1201 tgaacagcga aaccggcacg gaaaacagta ccgtttccta tgaagctgca ctcgccttgc
     1261 atatgcgggg gcgcgtcggt gaggccgagc atatgtacga ggcctttctc aagtcccacc
     1321 ccgaccatag cgaggcatgg cacggtctgg gcatcatccg cttgcgaagc gggcgcaccg
     1381 atacggcgat cgacaatttg cgcaaggcgg tcgctgccgg cggatcggcg gtcgtcaaaa
     1441 acaatctggg cgtggcgctg tgcgccgccc agcgcttcgc cgaggcggct gacgtgtatc
     1501 gcgcggtggt gcagagcgat cccgatgccg tttcatcact tggcaatctc ggacaggttc
     1561 tcaatcagct gcgggctttt gcggaagcgg tcgaggtctt gcagaagggg ctgcggctgg
     1621 ctccggataa tgcgcgcttg cacaatcagc tggctatcgc tctggccgaa tgccggcgcc
     1681 tcgacgaggc ccaggcgcat ttcgagaagg ccgtcgccct tgaccccggc cgaagcgaat
     1741 ttcattgcga tctgggggcg ctgctgctca agcgcgaccg gatccggcaa gccgccgagt
     1801 gctaccgtcg cgccctgtcg gtgacgcctg cctcgcccat ggcgctgtgc gggctgggcg
     1861 aggcgctggg cggcttgaac cgccacgagg aggctgtcgc ctgtttccgg caggccgttg
     1921 cgttggcgcc cggctatgcc gccgcccatt acaattgtgg caccgccttg acctatctcg
     1981 gccgcatggc cgaggcggaa acggcctttg tccgcgccgc ggaactggag cccgacaatc
     2041 ccgcttaccg cggcgctttg atcgccatga agaagacgac ggcttccagt gcgcatctca
     2101 aggcgctgca agccctggca atcgacgcag accgcctcgg cgagcgcgaa gccatggaac
     2161 tgcaatttac attggcgaag gcctatgacg atatcggcga ccatgcgcgt gcgttcgagg
     2221 cgctgcgtct cggcaacgcg gccaagcgcc gtcagaaccc ttacgaactg caacaggacc
     2281 tcgaccgttt ccgagccatc gccgatgcct tcacggccga attgcttgcg gcccatgccg
     2341 gtcagggcga tccgacggag gttccggtgt tcgtggtcgg catgccgcga tccggcacca
     2401 cgctggtcga gcagattctc gccagccatc ctgatgtgtt cggcgccggc gaactgacca
     2461 tcctgccgga cctgattagt tccgggcgcg cggggggcga ttttcccgcc gccgtcgcca
     2521 cgctcggtgc ggaggcgtgg cgcgccatcg gggaaaccta tgccggaacg ctgcgtgccc
     2581 aggcggccga ggcggtgcgc atcaccgaca agcttccgct caacttccaa ttggtcggct
     2641 tgatccgctt ggccctgccc aaggctcgca tcattcatgt gatgcgcgat cctttggata
     2701 cgtgtttttc ctgctatttc accttgttcg cccacgagct gggcttttcc gacgaccttg
     2761 cggatttggg acggtattac cgcggctatc agagcctgat ggcgcattgg cgcagcgtgc
     2821 tgctcgaagg cgcgatgctg gaaattcaat acgaaaatct ggtggcggat cttgaaaccg
     2881 aggcgcggcg gctgatcggc tattgcggtc ttccctggga cgcacgctgt cttgactttc
     2941 acaataccgt gcggccggtc gagaccgcca gcatgctcca ggtgcgccgg cctttgtttc
     3001 aaacctccgt cggccgggcc gggccctatc ggccgtggct ggcgccgctc gaacgggcgc
     3061 tggccggcga aacctgaaac cggcggatgg acggcgatgc aacaatacgg ccaggatgca
     3121 atggtaagcc ggctgttggc cgagggaatt cacctgtgcc ggacgggccg attcgcacag
     3181 gccaaggcga tttatgaccg gataatgatg ctggcgccgc agcgggtcga accgtatctc
     3241 aactgcggcc agatcgggct cgccgaaggg caatatgacg acgccttgtc ctggttcgag
     3301 aaggtccgcc gcctcaagcc ggatctgccg ccggcgttgg aaggcgccgg cgccgcactg
     3361 cttgcgctcg gccgtccggc cgaggcgctg gcgattttcg aaaggctgca aactctcgac
     3421 gccaagtcga gcgcagcgct tcacggcgcc ggcgaagcct tgaagcagct cggccggttg
     3481 aaggaggcgc gcgccgtgct ggagcgcgcg atcgaactgg cgccggatgt cgccggtcat
     3541 cattacctgc tcgcgcaact cgccaggttc gtcgagaatg atccgcgcct tgcagggctg
     3601 gaacggctga ggaacaaggc cgcgtccttg cccgaacccg atcgatgcca actccacttc
     3661 gcgctcggca aggcttacga cgaactcgga cggcatgcgc aggcgttcga acactggcgg
     3721 gccgccaatg cgatcaagcg gcgccacatt gcgtatgacg aatcgatgtt tctcggcatc
     3781 ctccgcgatc tggcggccgc cttcacccct gaggtgatgg ccgcgcgata cggtgcgggc
     3841 gatccgtcac ggctcccggt gttcgtggtc ggcatgccgc gctccggcac cacactggtc
     3901 gcgcagatca tcgccagcca tcccgacgct ttcaatgtcg gcgagagcat gtatttgcac
     3961 tatctgctcg ggcagaaccg ggcgggaccg gattttccgg cccatttcac ttcggttccc
     4021 aaagagggct tgcgcaatat gggcgcattc tatgtcgccc acctcaacgc gagcgcgcca
     4081 aaagcaagcc gcatcgtcga caagctgacg gcgaatttca tgctctgcgg cctgatccgc
     4141 atcatcctgc ccaacgcgcg gatcatccat gtcaggcgcg atccgcttga cacctgcctt
     4201 tcctgctacg ccaacctgtt cagccagaac atcgattaca gctacgacct cggcgagctg
     4261 ggccggtatt accgcgctta cgaaacgctg atggcgcact ggcgccgcat cctgcccgaa
     4321 ggcgcgatgc tcgaggtgag ctacgaagag ctggtgtcgg atttccccgc gcaagctaga
     4381 cggattgtcg gctattgcgg gctcggatgg gacgaagcct gcctttcctt tcacctgacc
     4441 aagcgcgtgg tgcatacctt gagcgccgtc caagtgcgcc agccggttta cgggagttcg
     4501 gtgggccgtt ggaaagctta tgcctcccat ctcggtccgt tgatcgaagc tctgggcgcg
     4561 gcccgataag gaccgcatgc cggccgaggc ggcgcgccct tgccgagcgc ctgcgacagg
     4621 cgtcgcaagc ccttgtttgc gttgacacct gtgccgcacc ccgcgcataa ttctctggca
     4681 tacccatccg gggaaaatgg ggggcatctc atgcgcattt ttgcggccat tgcggcgtgt
     4741 tcctgcctgt ttcttctatc ggctccggcg ttcggcgaaa cattcacttc ggaagagtac
     4801 ggcttcacgg cggagtttcc ggcgcggccg acggtcggcc cgccgcaagg ctcggaaacc
     4861 gactcaaagg gcaacttcat ctcgacgtcg gtgatgatca aaacgcaggt gctgggcgtc
     4921 tatacggcga tggtcaccgt ggactcttat cttgtgccgt tgaagatcga tccgtcgtcg
     4981 acgctgctgg cgatgtcgcg cagcttcgtg gcgcaactgg atgcgacgac cacgtcgtcc
     5041 aagccgggtc ggctcgacgg cttcagagcg cgcttcttct cgtacgacac ggcggaccat
     5101 tcggcgtcgg gcaaagggat cgtggtaatc gttccggcaa agaaaccacg gacgtatctt
     5161 gtcctcacca tgcatacgtc cgcggcgtcg gcggacgaaa ttgccgccct cgacaagttt
     5221 ctcgactcct tccacgtgaa gtagcttttg tcgtcccggc aagaggacga gcggaggatg
     5281 tttccgcgac aggtctctct gacgtcgcac aaggtttttg ccggccgatt tctctgttcg
     5341 atcacaaaga aatggcttga cacagataac tctgtcatac agagtaatct acagtgatca
     5401 gacggtggag tggtcgtgtc gtttgccggg aagcgttttg ccagacctgc tggtttgccc
     5461 atgcggcgat ttgtaccgag tttcacgata gacattccgc tcgatattaa tttacagcgt
     5521 atatatatac cgcagcagga cggtgatccc gctccgcttg tcacagaaaa gccttttttc
     5581 ccaatcggtt ggcatgccgc atcggacggc actgctggag tttcggaggc cgtaagccat
     5641 gaccgataaa tcagacgccg ggccgatcgt cagcctgcgc aacgtcgtca aaacctacag
     5701 cgacgacggg ctgaaagtat ccgccgtcaa cgacatcagc ttcgacatcc agcgccagcg
     5761 cttcaccatg atcgtcgggc cgtcgggcag cggcaagacc accttgctca acctgatcgg
     5821 ctgcatcgac gttccgacgg cgggcaccat cgaggtctgc ggcgagaagg tcgccaagct
     5881 ttccgacaac aagatcaccg atttccgcgc ccgcaacatc gccttcgtgt tccagaactt
     5941 caatctgtac ccggtcctgt cggcctacga gaacgtcgag tatccgctgc tcctgatcgg
     6001 catgccggcg aagcagcgca aggaacgcac gctggcgatg ctcgaatccg tcggcctggc
     6061 ggaccagcgg cggcaccgcc cgaaccagct c
//
LOCUS       DASWPU010000608         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_54099,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000608 DASWPU010000000
VERSION     DASWPU010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54099"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VGC27_12945"
     CDS             complement(<1..542)
                     /locus_tag="VGC27_12945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0283518.1"
                     /translation="MKRVANPRFLAALAALAALRLLAGALLPLSADEAYYWLWSRHLA
                     AGYFDHPPSVAFVIRAGTELFGATPFGVRIGSILLSFAASWFVWRTGAILGRDEKVGA
                     LACLFFNLTPMISVEMLAATPDAPSIATAAVFFWGLAKVSETKDGRWWLMAGAAAGLG
                     LLSKYSAFFLGLGTLVWLLA"
     gene            complement(539..1672)
                     /locus_tag="VGC27_12950"
     CDS             complement(539..1672)
                     /locus_tag="VGC27_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0283519.1"
                     /translation="MSLSTVAEKTDAAPDQARGVLLSVVIPTYKERGNVAELIKRLER
                     TLEGVAWEAVFVDDNSPDGTADAVKEIAARDPRIRCLRRVGRRGLAGACIEGMLSSCA
                     TYVAVMDADLQHDERILPEMLAKLQTGKFDLVAATRYVEGGSAGSFSEGRGKISRIAT
                     RLTQRILGVPLSDPMSGFFMMRRDSFDAVAGRLSPAGFKILLDIATAGSGLRIAEQPF
                     VFRSRNEGESKFNVQIGLEFLGLLFAKLSHGLVEPRFIFFALVGAVGIAVNLAVLNIA
                     MLVWPVSFMLAKSVATFFAMVSNFLLNNGLTYRDRRLRGWGMLRGFIGFCLIGAVGAL
                     TDVGLASQLYAEREVWWVAGLAGAVMGVLWNYAMSSMFIWRTR"
     gene            1911..3080
                     /locus_tag="VGC27_12955"
     CDS             1911..3080
                     /locus_tag="VGC27_12955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY0283520.1"
                     /translation="MPSIANSIIETRWLEALSRLEFGTLEFVAPNGEVHTAKGRQPGP
                     HARFVIHDWNVLSRIMARGDIALGEEYIAGAWETDDIETLVTLFLMNMEALEKYANGN
                     LLTRLALMIQDRFVRRNSLSGSKRNIEAHYDVGNEFYALWLDKSMTYSSALFGNTANS
                     LEQAQTNKYERILGKFLKPCSSVLEIGCGWGGFAERAAAHGHRLTGLTISPSQYRFAR
                     ERLKGAADILLEDYRKARGLFDMIVSIEMFEAVGEQYWPQYFRSVAERLKRGGRAVIQ
                     TITVRDELFSGYRLRSDFIRHYVFPGGMLPSLQRFREEAERAGLKVVDSFSFGKDYAK
                     TLREWSSRMREAKNEIFALGYDERFLRNWQFYLGICAASFSVERTDVVQVELVNA"
BASE COUNT          676 a         1082 c         1037 g          568 t
ORIGIN      
        1 ctggcgagaa gccaaaccag ggtgccgagg ccgagaaaga acgcggaata tttggacagc
       61 agcccgaggc cggcggcggc acccgccatc agccaccagc gcccgtcttt ggtttcggag
      121 accttcgcca agccccagaa gaaaaccgcc gccgtggcga tggagggcgc atcgggcgtc
      181 gccgccagca tctccaccga aatcatcggc gtgagattga agaagaggca ggccagcgcg
      241 cccaccttct cgtcgcgccc caggatcgcg ccggtgcgcc agacgaacca gctcgccgcg
      301 aacgacagca ggatggagcc gatacgcacg ccgaacggcg tcgcgccgaa cagttcggtt
      361 ccggcgcgga tgacgaaagc gacggaaggg ggatgatcga aatagcccgc cgccagatgg
      421 cgcgaccaca gccagtaata ggcctcgtcg gcggacagcg gcagcagcgc gcctgcgagc
      481 aggcgcaagg cggcaagagc cgccaacgcc gcgaggaacc gcgggttggc gacgcgcttc
      541 atcgcgtgcg ccagatgaac atgctggaca tggcgtaatt ccacagcacg cccatgacgg
      601 cgcccgcaag gcccgccacc caccagactt cgcgctcggc ataaagctgc gacgccaagc
      661 caacatcggt gagcgcgccg accgcgccaa tcaggcagaa gccgatgaaa ccgcgcagca
      721 tcccccagcc gcgcagccgc cggtcgcgat aggtcaggcc gttgttgagc aggaagttgc
      781 tcaccatggc gaagaaggtg gcgacggact tggctagcat gaaagatacc ggccaaacca
      841 gcatcgcgat gttgagcacg gccagattca ccgcgatgcc gaccgcgccg accagggcga
      901 agaagatgaa ccgcggctcg accagcccgt gcgacagctt ggcgaacaga aggcccagga
      961 actccagccc gatctggaca ttgaatttgc tttcgccttc attgcggctc ctgaagacga
     1021 aaggctgttc ggcaatgcgc agtccgctcc cggcggtggc gatgtcgagc aggatcttga
     1081 accccgcggg cgacagcctt ccggcgacgg cgtcgaagga gtcgcggcgc atcatgaaaa
     1141 agccgctcat cggatcgcta agcggaacgc cgagaatccg ctgagtaaga cgggtggcga
     1201 tgcggctgat cttgccgcgt ccttcgctga aggagccggc gctgccccct tccacatagc
     1261 gcgtcgccgc gaccagatcg aatttgccgg tttgcagctt cgccagcatc tcgggcaaaa
     1321 tccgttcgtc gtgctgcaaa tccgcgtcca tcaccgcgac ataggttgcg caggatgaca
     1381 gcatgccttc gatgcaggcg ccggccaggc cgcggcggcc gacccggcgc aggcagcgaa
     1441 tgcgcggatc gcgcgcggcg atctccttaa ccgcgtcggc cgtgccgtcg ggcgagttgt
     1501 cgtcgacgaa aaccgcttcc caggcgacgc cttccagcgt gcgctccagc cgcttgatca
     1561 gttcggcgac gttgccgcgc tccttgtagg tcgggatgac gaccgaaagc agcacgcccc
     1621 ttgcctgatc gggcgcggca tccgtttttt cagcgacggt ggaaagcgac atggcgccag
     1681 cgactcctcg atggggcgct ttggtgaccg cacccggccc tccgcgtcaa gagccggccg
     1741 gccgcaggcc gcaccaaccg gatctttacc gacaaatatc aattatatca atatgttact
     1801 tgctttctgc caaagcaagc ttttactttt ctcctacctc gcaggttggt ttaagatgga
     1861 ttcgacgagc gttcctacgt ctcacgtctc aaatgacagg cgggattgtg atgccttcga
     1921 ttgcgaactc catcatcgaa acacgctggc tcgaagcttt gtcgcggctg gaattcggca
     1981 ccctcgaatt cgtggcgccg aacggcgaag tgcacaccgc gaagggccgc cagcccggtc
     2041 ctcatgcgcg cttcgtcatc cacgattgga acgtgctgag ccgcatcatg gcgcgcggcg
     2101 acatcgcctt gggcgaagaa tatatcgccg gcgcctggga aaccgacgac atcgaaacat
     2161 tggtgacgct gttcctgatg aacatggagg cgctggagaa atacgccaac ggcaacctgt
     2221 tgacccgcct cgccttgatg atccaggacc gcttcgtccg gcgcaattcg ttgagcggat
     2281 cgaagcgcaa catcgaagcc cattacgacg tcggcaacga gttctacgcg ctctggctcg
     2341 acaagagcat gacctattcc tccgccctgt tcggcaacac cgccaacagc ctggagcagg
     2401 cgcagaccaa caaatatgag cgcatcctgg gcaagttcct caagccctgt tcctcggtgc
     2461 tggagatcgg ctgcggctgg ggcggcttcg ccgaacgcgc ggcggcgcac ggccatcgcc
     2521 tcaccggcct gacgatttcg ccttcgcaat accgcttcgc gcgcgaacgg ctgaaaggcg
     2581 cggccgacat ccttctggaa gattaccgca aggcgcgcgg cctgttcgac atgatcgtct
     2641 ccatcgagat gttcgaagcg gtcggcgaac aatattggcc gcaatatttc cgcagcgtgg
     2701 ccgaacggct caaacgcggc gggcgcgcgg tgatccagac catcaccgtc cgcgacgaat
     2761 tgttctccgg ctaccggctg cgcagcgact tcatccgcca ttacgtcttc ccgggaggca
     2821 tgctgccgtc cctacagcgc ttccgcgagg aagcggagcg cgccggcctg aaggtggtgg
     2881 acagtttttc cttcggcaag gattacgcca agaccctgcg cgaatggtcc tcgcgcatgc
     2941 gcgaggcaaa aaacgaaatc ttcgcgctcg gctacgacga acgtttcctg cgcaactggc
     3001 agttttatct cggcatctgc gcggcgtcgt tcagcgtcga acgcaccgac gtggtgcagg
     3061 tcgaactggt caacgcataa atccgcccgc ccgtctccgt cgcgcaccgg cttgcgcagc
     3121 ggggtctttt ttccacatgc gattgaatca cagtcgcgtt gcacgcggtc ggctgtgcca
     3181 atccgcctgc tggagcaggc gtgaaatgag tcctagagat gaatgtttgg ggcacaaagg
     3241 cagcgctaag cgatgacctt atcctggggg cgcaacggac cggcggcggc gcttgcatta
     3301 gcggcaggcg tactcatcgc tccgctatgg tgcgtgtatc ttcgcctgcc ggcagacctg
     3361 ggc
//
LOCUS       DASWPU010000609         6975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_97450,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000609 DASWPU010000000
VERSION     DASWPU010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97450"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..915
                     /locus_tag="VGC27_12960"
     CDS             79..915
                     /locus_tag="VGC27_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY0283521.1"
                     /translation="MRAQASFANLAVASRRIERRSGPRLYDRVVVGGLRTACVSRRQL
                     GRLMIGDCLAARGGKRSPKLVFASNGHAVALAAMNPAFRRQMEQADLIHADGTPIVFA
                     SRMLTDTPVPERSATTDFIFDAAGVAGQNGLKFFLLGSTEEINARCAEILRQTYPGVE
                     IAGRRNGYFAADEEAQVCDEINRSGADVVWVGLGVPLEYEFCLRNKDRLKVGWIVTCG
                     GCFNFAAGEYVRAPIWMQKAGLEWLHRLWCEPKRLFWRYLITNPVAMAMLLLKTASAK
                     AG"
     gene            983..2227
                     /locus_tag="VGC27_12965"
     CDS             983..2227
                     /locus_tag="VGC27_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283522.1"
                     /translation="MPAILRAVTRYGLSVTGPVTVSAAHFVASLIFLRLLSAHEFGQF
                     SFLLIVVPFCLSAGGALLGAPAALTRGKDEATARTEISTLQKASLAVSGVAGLAVAAL
                     MFSTHAGVTEAILFGLYGAGATLRAFARSLANIHARLLRVAGSDIVYAVLLVAGLAGL
                     IVFGRLTMLNAAMVLVLSTLASFLPFGWRHWRDLIRSVATQPFGRYLPMWQDVTRWSL
                     LGVVLTELTANAHAYFVTFISGPQVFGLLALGALFMRPASLVLGALPDIDQPLMTRRI
                     AQGDFRGALRVVKEFRTAAGAVLAATIALSVALVNWYPQLFLKKGYDESDVMIVLALW
                     IAITVVRVLRTPEAVLMQAVGKYSTLARVSGWSSAVSLAVTFVLLVTVGPVASLGGVL
                     AGEIVVVLVLFPMTRAWRRQHA"
     gene            2220..3149
                     /locus_tag="VGC27_12970"
     CDS             2220..3149
                     /locus_tag="VGC27_12970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0283523.1"
                     /translation="MLDVVVAIPTFRRPRSLARLLAALEKIETEAHIVVVVADNDAER
                     HEGYDLCLTLRAQGYRWPLDPVIAAERGIAQVRNVLVERALSYPDAGFVAMLDDDEWP
                     SPCWLDEFLRVQRQTGADALQGSILFESVSAPKSWAVGFDGISSIRRPTGPVKMIEGA
                     GNLLLTRACLESLEAPWFDPAFALTGGEDREFFERLAQAGKRFAWADEAIARAVVPAA
                     RTGLKWMLARAYRIGNSDMRIFLKHRPKRLARAKEVAKIALTLLLSPLLLVILAAVPN
                     RAADALRRLFRNLGKTAALLGRHYNEYAVTHGE"
     gene            3139..4437
                     /locus_tag="VGC27_12975"
     CDS             3139..4437
                     /locus_tag="VGC27_12975"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HEY0283524.1"
                     /translation="MVNEGLAIGLNRARPASWIDRAVLLAFLLLAFVGLSPFSPPPPE
                     VTAFGGVATTGAGDLLRQVCYLAVFAAIIIGAVRANGLNAFSPVPILLALLLVWCLAS
                     ALWSGEPGVTLRRAGLAVVLVISALLAVETVGAERSLVLWRWVLAAVLAVNVASIHFI
                     PQAVHLAGEADPSLTGAWRGLYGHKNIAGSVGAMTAIVFLFAPHPSPGLFRKVLDVAI
                     AVAAVGFTVMTRSKSSLGLLAVALIFGVVYRVAWKRDLDRTIVIVMGALLAIVAAVFV
                     IADQSIIARLFQDPREFTGRIEIWAAQIAYIRDHALIGAGFGTFSDTGGLSPLRNYIG
                     DSWVGAASHGHNGYLQLLVTIGAVGFLLAFAGLIVAPALTFWKRGGAVEMKALLFALF
                     AFLLLHNLMETDFLEGDGVTWVGYLLMLGMLGNLARRGLA"
     gene            4437..5369
                     /locus_tag="VGC27_12980"
     CDS             4437..5369
                     /locus_tag="VGC27_12980"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0283525.1"
                     /translation="MTTMPFFSVVIPVYNRAALLGRALRSVLAQSEQDFEIIVIDDGS
                     ADNPSRVVEELADPRIVFVRQDNRGGGAARNTGIAKARGRFIAFLDSDDRFFPHHLAT
                     MRKLLENAPGTAGYARILVDRGEGRIFLKPPRAIRAGEDMATYLLCDRGFLATSTIVV
                     ERKMAEVIRFHENLAAAEDTDFAIRLALAGQRFVMAEEPGVHWNDLSDPNRLSAGRRS
                     ATMRQWIEDLRPRIPAKAYYGCLGWAYAKYVAMNNRFAALKLYLAALVRGCYRPSLAG
                     IVFLQIFLPDSAYRKLADTAIARLGAGLRPGSAP"
     gene            5391..6659
                     /locus_tag="VGC27_12985"
     CDS             5391..6659
                     /locus_tag="VGC27_12985"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0283526.1"
                     /translation="MVDHRLLCWGRMTQRILLATTVKWPSAAYLANAFASFGCSVEAV
                     FPRRHVLGVSRDVARAHAYHPLRPHASFAAAIAAAKPDLIVPCDDRAVLHLLALQVRT
                     PQIAALLVRSMGRLESYPAMMARSSAIIAAQAEGIAAPLTIAVANEAEFARALDRVGL
                     PAVLKADGSWGGDGVAVVRTREEAGLAFRKLADPPSRLRSLTRAVLRKDAHFLGAAFV
                     PKAAPVQVQRFIEGKPATSAFACREGEVLAAIHMNVVEAQGTTGPAALIERTDCARMD
                     KAARRIARRFGLSGLHGLDFVRDEDGIPHLIEINPRATQICHLALGAGRDLPSALLGR
                     RPRPAVTDKALIALFPQAWSTGRLPPNASAAYLDVPWDDPAVVSAALGPGISLLKAQP
                     GRKAPAASDTLDFLTGRLEESDVRSHRLPL"
     gene            6774..>6975
                     /locus_tag="VGC27_12990"
     CDS             6774..>6975
                     /locus_tag="VGC27_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283527.1"
                     /translation="MAIDRTASTFASTASTPFEAAFGFDDFLRVLDERRSLIRNVTLA
                     VAALTLLVLFTLPTLYSSSAVII"
BASE COUNT         1012 a         2239 c         2263 g         1461 t
ORIGIN      
        1 ccacgcgagc ttcggcctcg acgtcgagat cctgctccgc acgcctctgg aaatccttcg
       61 ccgcaggaac gcctattgat gcgcgcgcag gcgtcattcg cgaacctcgc cgtcgcctcc
      121 cggcggatcg agcgccgctc cggaccgcgg ctttatgatc gcgtcgtcgt gggcggcttg
      181 cgcacggctt gcgtgtcgcg ccgccaactc ggcagactga tgataggcga ttgcctcgcg
      241 gcgcgcggcg gcaagcggtc gcccaagctc gtttttgcct cgaacggcca tgccgttgcg
      301 cttgcggcca tgaatcccgc cttccgccgg cagatggaac aggcggattt gatccacgcc
      361 gacggcacgc ccatcgtctt cgcgtcgaga atgctgaccg atacgccggt gccggaacgc
      421 agcgcgacta cggatttcat cttcgatgcc gccggcgtcg ccgggcagaa tggattgaaa
      481 ttctttctcc tcggttcgac cgaggagatc aacgcgcgct gcgccgaaat tctgcgccag
      541 acttatcccg gcgtcgagat cgcgggccgg cgcaacggct atttcgcggc cgatgaggaa
      601 gcgcaggtct gcgacgagat caatcgttcc ggcgccgacg tggtgtgggt cgggcttggg
      661 gttccgctgg aatatgaatt ctgcctgcgc aacaaggaca ggctcaaggt cggctggatc
      721 gtgacctgcg gcggctgctt caatttcgcg gccggcgaat atgtccgcgc gcccatttgg
      781 atgcagaagg ccggcctcga atggctgcac cggctatggt gcgaacccaa gcggttgttc
      841 tggcgctatc tgatcaccaa tccggtcgcc atggcgatgc tgctgctgaa gaccgccagc
      901 gcgaaagccg gataggggat cggccatgtc cgttggcgcg cagctcgcac ttcttctgcc
      961 ccgaggtgtt gatcggcttc ggatgccggc gattctgcgc gccgtcaccc gctatggctt
     1021 atccgtcacc ggtccagtca ccgtctccgc cgcgcacttc gtcgcttcac tgatctttct
     1081 gcgcctgctg tcggcgcatg aattcggcca attctcgttc ttgctgatcg tcgtgccgtt
     1141 ttgcctgagc gccgggggtg ctctcttggg tgcgccggcc gcgctgacgc gcggtaaaga
     1201 cgaggccacg gcgcgtaccg aaatctccac tttgcagaag gcgagcctgg ccgtttccgg
     1261 cgttgccggg ctggccgtcg ccgctctgat gttctccacc cacgccggtg tcaccgaagc
     1321 gatcctgttc ggcctctacg gcgcgggcgc gaccctgcgc gctttcgccc gcagtctcgc
     1381 caatatccac gcgcggcttc tgcgggttgc aggttcggat attgtctatg cggtactgct
     1441 ggtcgcgggc cttgccgggc ttatcgtttt cggccgcctg accatgctga acgctgcaat
     1501 ggtgctggtg ctgtccacgc tggcgagctt tctgcccttc ggctggcgcc attggcgcga
     1561 tctgatccgt tcggtggcga cgcagccgtt tggccgctat ctgccgatgt ggcaggacgt
     1621 gacgcgctgg tcgctgctcg gcgtcgtcct caccgaactg acggccaacg cccacgccta
     1681 tttcgtgacc ttcatttccg gcccccaggt attcggcctt ctggcgcttg gcgcgctgtt
     1741 catgcgcccg gcctcgctgg tgctgggggc gctgcccgac atcgaccagc cgctgatgac
     1801 gcggcgcatc gcccaaggcg atttccgcgg cgcgcttcgg gtggttaagg aatttagaac
     1861 ggcagccggc gcagtgttgg ccgccaccat cgccctttcg gtcgcgctcg tgaactggta
     1921 tccgcaactc ttcctgaaaa agggctacga cgagtccgac gtcatgatcg tgctcgctct
     1981 gtggatcgcg attacggtgg tcagggtgct gcgcacgccc gaggcagtgc tgatgcaggc
     2041 cgtgggcaaa tattcgacgc tggcgcgcgt cagcggctgg tcgagcgcgg tgtcgcttgc
     2101 cgtcactttt gttctcctgg ttacggtagg tccggttgcg tccttgggcg gcgtgctggc
     2161 gggcgagatt gtggttgtgc tggtgttgtt cccgatgacg cgggcctgga ggcggcaaca
     2221 tgcttgatgt cgtcgtcgcc attccgacct tccgccgtcc gcgcagtttg gcgaggcttc
     2281 tggcggcgct ggagaagatc gagaccgagg cgcatatcgt cgtggtggtc gccgacaacg
     2341 acgccgaacg tcacgaaggc tacgatctct gcctgacgtt gcgcgcgcaa ggctatcgct
     2401 ggccgctcga tcctgtgatt gcggccgagc gcggcatcgc gcaggtgcgc aacgtgctgg
     2461 tcgagcgcgc actgtcctat cccgatgccg gtttcgtggc gatgctcgac gacgacgaat
     2521 ggccttcgcc gtgctggctc gacgaattcc tgcgcgtgca gcggcagacc ggcgcggatg
     2581 cgctgcaagg ctctatcttg ttcgagtccg tctccgcccc gaaatcatgg gccgtgggct
     2641 tcgacgggat aagttcgatc cgtcggccca ccgggccggt gaagatgatc gagggagcgg
     2701 gcaatctgct gttgacgcgc gcctgcctgg aaagcctgga agcgccctgg ttcgatccgg
     2761 cgtttgcgct gaccggcggc gaggatcgcg aattcttcga gcggctggcg caggcgggca
     2821 agcgcttcgc ctgggcggac gaggccatcg cccgcgccgt cgtaccggcc gcgcgaaccg
     2881 gcctgaaatg gatgctggcg cgcgcctacc ggatcggcaa ttccgatatg cgtatcttcc
     2941 tcaaacatcg cccgaagcgt ctcgcccgcg ccaaggaagt cgccaagatc gccttgacgc
     3001 tcttgttgtc gccgctcttg ttggtcatcc ttgcagcagt tccgaatcgc gccgccgatg
     3061 cgctgcgcag gttgttccgg aacttgggaa agaccgcggc gcttctgggc cggcattata
     3121 acgagtatgc cgtcacgcat ggtgaatgaa ggtctggcca tcgggttgaa ccgcgcgcgt
     3181 ccggcgtcgt ggatcgatag agctgtcctt ctggcgtttc tgcttctggc ctttgttggc
     3241 ctatcgccct tctcccctcc tccgcccgag gtgacggcgt ttggaggggt tgccacgacc
     3301 ggagcgggcg atctgctgcg ccaagtgtgc tacctcgcgg tgtttgctgc catcatcatc
     3361 ggcgccgtca gagccaacgg actgaacgcg ttctcgccgg tgcccatcct gctcgcgctg
     3421 ttgctggttt ggtgtttggc gagcgccttg tggtccggcg agccgggcgt cacgctgcgc
     3481 cgcgccggac ttgccgtcgt cctggtgata tccgcgctgc tggccgtgga aacggtcggc
     3541 gccgagcgca gtctggtact gtggcgctgg gtgctggccg ccgttctggc cgtgaacgtt
     3601 gcctcgatcc attttattcc ccaggcggtg catttggcgg gcgaggccga tcccagcctg
     3661 acgggtgctt ggcggggtct ctacggccac aagaacatcg ccggctccgt cggcgcgatg
     3721 accgccattg ttttcctgtt tgcgccgcac ccttcccccg ggctgtttcg caaagtgctg
     3781 gatgtggcga ttgccgttgc ggcggtcggc ttcaccgtga tgacgcggtc gaaatcctcg
     3841 ctcggcctgt tggcggtcgc gcttattttc ggcgtcgttt atcgcgtcgc ctggaagcgg
     3901 gatttggacc gcacgatcgt gatcgtgatg ggcgcgctgc tggcgatcgt tgcggcggta
     3961 ttcgtgattg ccgaccagtc gatcatcgcg cgtctatttc aggatccgcg ggagtttacc
     4021 ggccgcatcg aaatctgggc ggcccagatc gcttacatcc gcgatcacgc cttgatcggg
     4081 gccggcttcg gtaccttctc cgacaccggc gggctgtcgc cgctgcgcaa ttatatcggc
     4141 gattcctggg tgggcgcggc cagccacggc cacaacggct atctgcaact gttggtgacc
     4201 atcggcgcgg tggggttcct tctggctttc gcaggactca tcgtcgcgcc ggcgctgacc
     4261 ttctggaaac gcggcggcgc cgtcgagatg aaggcgctgc tgttcgcgct gttcgcgttc
     4321 ctgctgctgc acaatttgat ggaaaccgat tttctcgaag gcgatggagt cacctgggtc
     4381 ggctatctct tgatgctcgg catgctcggt aatctcgccc gccgaggact ggcctgatga
     4441 cgacgatgcc cttcttctcg gtggtaatcc ccgtttacaa ccgcgcggcg ctgctgggcc
     4501 gcgccttgcg ctcggtgctg gcgcagagcg aacaggattt cgagatcatc gtgatcgacg
     4561 acggctccgc cgacaatcct tcgcgcgtag tcgaggagct tgccgatccg cgcatcgttt
     4621 tcgtccgtca ggacaatcgc ggcggcggtg cggcgcgcaa caccggcatc gccaaagcgc
     4681 gcggacgctt catcgccttt ctggattccg acgaccgctt ttttccccac catctggcaa
     4741 cgatgcgcaa gctcttggag aacgcgccgg gcacggcggg ctatgcacgg attttggtgg
     4801 accgcggcga agggcgcatc ttcctcaaac cgccgcgcgc gatccgcgcc ggcgaagaca
     4861 tggcgacata tctgctatgc gaccgcggct tcctcgccac cagtacgatc gtggtggagc
     4921 ggaaaatggc cgaggtcatc cgctttcatg agaatctggc cgccgccgag gacacggatt
     4981 tcgccatccg cttggcgctt gccggtcagc gcttcgtgat ggccgaggaa cccggcgtac
     5041 attggaacga tctcagcgac cccaaccgtt tgtcggcggg gcgccggagc gcgacgatgc
     5101 gccaatggat cgaggacctc aggccccgga ttccggccaa ggcttattac ggctgtctcg
     5161 gctgggccta tgcgaaatat gtggcgatga acaaccgctt tgccgcgctg aagctttatc
     5221 tggccgcgct tgtgcgcggc tgttaccgcc cgtcgctggc cgggatcgtc ttcttgcaga
     5281 tattcctgcc cgacagcgcc tatcgaaaac tggccgatac cgcgattgca cggcttggcg
     5341 cgggcttacg ccctgggtct gctccctgac gccggcaggc gcgttcacgg atggttgacc
     5401 atcgcctcct atgctggggc cgcatgacgc aaagaattct cctggccacc acggtcaaat
     5461 ggccgtccgc cgcttatctg gcgaacgcct tcgcttcgtt tggctgttcc gtggaagccg
     5521 tattcccacg ccgccatgtt cttggcgtga gccgcgatgt cgcccgtgcg cacgcctatc
     5581 atccgctgcg tccgcatgct tcttttgccg cggcgattgc ggccgcaaag ccggacctga
     5641 tcgttccttg cgatgatcgc gccgtattgc atctgctggc gctccaggtg cgcacgcccc
     5701 agatcgccgc gttgcttgtc cgctccatgg gaaggctgga atcctatccg gcgatgatgg
     5761 cgcgcagcag cgccatcatc gccgcgcaag cggaaggcat tgcggcaccg ctcaccatcg
     5821 cggtggcgaa cgaagcggaa tttgcgcgcg ctttggaccg cgttggcctg ccggcggtgt
     5881 tgaaggcgga cggaagctgg ggcggcgacg gcgttgccgt ggtgcggacg cgcgaagaag
     5941 caggcttggc ctttcgcaag ctcgcggacc cgccctccag actgcgtagt ctcacgcgtg
     6001 cggtgctgcg caaggacgcg catttcctgg gtgcggcgtt tgttccgaaa gccgcgccgg
     6061 tgcaggtgca gcggttcatc gagggcaagc cggcgaccag cgcctttgcc tgccgtgagg
     6121 gagaggtgct ggcggccatc catatgaatg tcgtcgaagc gcaagggaca accggccccg
     6181 ccgcgctgat cgaacggacg gattgcgccc ggatggacaa ggcggcccgc cggatcgctc
     6241 gccggttcgg gctcagcggc ctgcacgggc tggatttcgt ccgcgatgag gacggcattc
     6301 ctcatctcat cgagatcaac cctcgtgcga cccaaatttg ccatttggcc ctgggggcag
     6361 gccgcgatct gccgtctgcg ctgctggggc ggaggccgcg cccggccgtc acagacaagg
     6421 ccctgatcgc gctttttccc caggcatgga gcactggccg cctgccgccc aatgcctctg
     6481 ccgcctatct cgatgtcccc tgggacgatc cggcggtggt ttccgccgcg cttggtccgg
     6541 gcatctcctt gctcaaggcg cagcccgggc gaaaggcgcc tgccgcctcc gataccctcg
     6601 atttcttaac aggccggctg gaagaaagtg acgtgcgttc gcaccggctg ccgctttgat
     6661 aagtttacgc cccgccccgg tgttaaccat ttatcagcgc aatcggctgt aaatcggttc
     6721 aaggcgccga aggaaggctc gcagccgacc cgaagcacag ggcaactcgg tccatggcca
     6781 tcgacagaac cgcgtcaacc tttgcttcaa cggcatcgac accgttcgag gcggccttcg
     6841 gttttgacga tttcctgcgc gtcctcgacg aacggcgaag cctgatccgc aacgtcacgc
     6901 tggcggtcgc cgcgctgacc ttgctggtgc tgttcactct gccgacgctg tactcgtcgt
     6961 cggccgtcat catcc
//
LOCUS       DASWPU010000610         5817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_60522,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000610 DASWPU010000000
VERSION     DASWPU010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60522"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VGC27_12995"
     CDS             complement(<1..323)
                     /locus_tag="VGC27_12995"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0283528.1"
                     /translation="MTYILYGDKGSGAFCVEAALAEAGAPYEFQTISLEKNEQKAPAF
                     LALNPSGKIPALRLPSDEIVTETAALLVIVAERHAQAALLPPLGTPERAQALRWIAFM
                     ASEIYP"
     gene            complement(394..1011)
                     /gene="rpsD"
                     /locus_tag="VGC27_13000"
     CDS             complement(394..1011)
                     /gene="rpsD"
                     /locus_tag="VGC27_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY0283529.1"
                     /translation="MSKRATAKFKIDRRMGENIWGRGKSPVNKRSYGPGQHGQRRAKK
                     LSDYGTQLQAKQKLKGYYGNITERQFRKYYTKASRRPGDTGENMIGLLERRLDAVIFR
                     AKFAPTPFSSRQLVSHGHVRVNGKRITVSSYIVKEGDEIALTPKARDLALVIDAAKSP
                     ERDTPDYLEVDHGKMAAKFLRTPKLSDVPYAVQMQPHLIVEFYSR"
     gene            1237..1325
                     /locus_tag="VGC27_13005"
     tRNA            1237..1325
                     /locus_tag="VGC27_13005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1271..1273,aa:Leu,seq:taa)
     gene            complement(1469..2296)
                     /locus_tag="VGC27_13010"
     CDS             complement(1469..2296)
                     /locus_tag="VGC27_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283530.1"
                     /translation="MTAFRSATKRACRAALLILLLCAVPGLLPAQAAEAPVAPEWAQP
                     GSATHVQVAPPAGFSRATTNFNTPIGIFEGQSDVGSALVPGSASYDPATRQYTINSAG
                     YNVWYTRDEFRYLWKKMSGDVSLAADIAYPDPKGYDDRKAVLVIRQSLDDDSKEALVA
                     LHGGGMIHLAQRPEKGARVKDMEYRIGGRGRPGGASPDSLVPVIAQRIGIEKRGDSFA
                     LFVSLEGEPMHQFGPPIALHIDGPFYVGIGFCSHLPVTPDTAVLSNVVLENEAGKVK"
     gene            2601..3536
                     /locus_tag="VGC27_13015"
     CDS             2601..3536
                     /locus_tag="VGC27_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0283531.1"
                     /translation="MNLSRFVAPSTFALIAAAIVAQAAPVRPPITSVSHLSVYSADAA
                     KTEYFYVHNLGAVKRDDPENAKGVRYYFNALQFIEVLPLPEAEAKSANRMDHVAYNTP
                     DVKAMKRYLAAHGVKAQDAIRKGADGSQWFDVSDPEGNIVQFVQPPKAPQPVADSPLS
                     NHIIHVGFIVHDRTLEDGFYLKLLGFRPYWYGGKEGEKPAWISAQVPDGTDWMEYMMV
                     SGPQTRGIPAAMSQSTAGVLNHFSLGVANMEKTVTLLREGGRISEKNDGPKIGADGKW
                     QFNTYDPDGTRAEFMEFQAVVKPCCSPFTASDPEK"
     gene            4178..4894
                     /locus_tag="VGC27_13020"
     CDS             4178..4894
                     /locus_tag="VGC27_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283532.1"
                     /translation="MLRLRIAVMVLGAMAILTTSAPAQDAGSLETVVVTGARAEYDAQ
                     TVPHVFLLRRADHLITKVRVVCDTRELSKRRQELKDTLRNMIREASKSGTISLGVGDE
                     IVADLTEKMLDKVIAPDARADTSIAAVVVKTRVTKEDAFDDATGRIERFIEATPKSGR
                     TEILRVNRWDLTIVGPEQYRAALIAKVAKDAKQTAALFGADYAVRIEGLQHPIAWYQK
                     GPLDLALYIPYTLTIAPPVR"
     gene            complement(4934..5446)
                     /locus_tag="VGC27_13025"
     CDS             complement(4934..5446)
                     /locus_tag="VGC27_13025"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY0283533.1"
                     /translation="MKLRTLLMALSVLPFLAGASIAATAPKVHIASLADLPVVERSPY
                     NPNANATAAVDAAFARAKKNGKRVLIDLGGNWCPDCIVLANLMQLPEMKPFLAAHFEI
                     VAVDVGRFDKNLQIPARFGITKRLEGVPSVIIAEPNGKFVNPGRIAALADARHMTPQA
                     IADWLAQWAK"
     gene            5620..>5817
                     /locus_tag="VGC27_13030"
     CDS             5620..>5817
                     /locus_tag="VGC27_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283534.1"
                     /translation="MKRIVLVAALAFLPALAPAYAADPVQPAMMWDLGDLYASPEAWS
                     AAHAKALAEAQKLDGYKGTLAN"
BASE COUNT         1115 a         1765 c         1865 g         1072 t
ORIGIN      
        1 ggatagattt cggacgccat gaacgcaatc cagcgaaggg cttgggcgcg ctccggcgtg
       61 ccaaggggag ggagaagcgc cgcctgcgca tgacgttcgg cgacgatcac aagcaaagcc
      121 gccgtttcgg tgacgatttc atcggacggc aaacgcagcg ccggaatctt gccgctcgga
      181 ttgagcgcca gaaacgcggg cgctttctgt tcgttctttt ccagcgaaat cgtttggaac
      241 tcgtagggcg cgcccgcttc ggccagcgcc gcttccacgc agaacgcccc ggaacccttg
      301 tcgccgtaaa gaatgtaagt catcgcgcgc ccctttaaac aaaaagcccc gcgatattcg
      361 cggggctttc cgaaattgca agctcgcgat gtcttaccgc gaatagaact cgacgatcag
      421 atgcggctgc atctgcaccg cataaggcac atcggaaagc ttcggcgtgc gcaggaactt
      481 ggccgccatc ttgccgtgat cgacttcgag gtaatcgggc gtatcgcgct cggggctctt
      541 ggcggcgtcg atcaccagcg ccaggtcgcg ggccttgggg gtcagcgcga tctcgtcgcc
      601 ttccttgacg atgtaggacg aaaccgtgat gcgcttgccg ttgaccctga catggccgtg
      661 ggagacgagc tggcgcgagg agaacggcgt gggcgcgaac ttggcgcgga agatcaccgc
      721 gtcgagccgg cgctccaaca ggccgatcat gttctcgccg gtgtcgccgg ggcggcgcga
      781 agccttggtg tagtatttgc ggaactgccg ttcggtgatg ttgccgtaat agcccttcag
      841 cttctgcttg gcctggagct gggtgccgta gtccgacagt ttcttggcgc ggcgctgacc
      901 gtgctggccg gggccatagg agcgcttgtt cacgggactc ttgccgcggc cccagatgtt
      961 ttcgcccata cggcggtcga tcttgaattt ggcagttgcg cgcttgctca ttcttcgtcc
     1021 agattttgcg gaaagccgct ggtttggagg cgggttcgcc ccgagaaagg cgggcaatat
     1081 agcggccgga ctttgcctgt caatggcgtc caaactattg ggatgtttgg gcttttgtga
     1141 ggagactcga ctacaagttg caattgacgc acaagtcttg cggcatgcgt gtgcttgcgg
     1201 cggcgagagt tttgacgcaa ctcagcccgc gatttcggcc ccgtggcgga actggtagac
     1261 gcaacggact taaaatccgt agttccgaaa gggacgtgac cgttcgactc ggtccggggc
     1321 caccacccta cgctgctgcg cagcttcggg tggctacgcc acaatttcaa ttcagcgaag
     1381 gcggaagtcc gcaagggcga aggagggctg ctgatgggac cgcgcggctt cgggtggcaa
     1441 gccgcccttc gcttcgtcac attcggcgct acttcacctt ccccgcttcg ttctcgagaa
     1501 cgacattgga aagcacggcc gtatccggcg tgaccggcag gtgcgagcag aacccgatgc
     1561 cgacatagaa cggcccgtcg atgtgcaagg cgatgggcgg gccgaactgg tgcatcggtt
     1621 cgccttccaa gctgacgaag agggcgaaag agtcgccgcg cttttcgatg ccgatgcgct
     1681 gggcgatcac gggaacgagg ctgtcggggc tggcgccgcc gggacggccg cggccgccga
     1741 tacggtactc catatccttg acgcgagcgc ccttctcggg acgctgcgcc agatggatca
     1801 tgccgccgcc atgcagggcg acgagagctt ccttggaatc gtcatcgagg ctctggcgaa
     1861 tgacgagaac ggccttgcgg tcgtcatagc ctttggggtc cgggtaggcg atgtcggcgg
     1921 cgagagaaac gtcgccggac atcttcttcc acagatagcg gaactcgtcg cgcgtgtacc
     1981 agacgttata accggcggag ttgatggtgt actgccgcgt ggcggggtcg taactggcgc
     2041 tgcccggcac caaggcgctg ccgacatcgg actggccctc gaaaatgccg atcggagtgt
     2101 tgaaattcgt cgtggcccgg ctgaagcccg ccggcggcgc aacctggaca tgcgttgcgg
     2161 aacccggctg cgcccactcc ggcgcaaccg gcgcttcggc ggcttgcgcg ggaagcaaac
     2221 cgggcacggc gcaaagcaac aaaatcaaca gtgccgcgcg gcacgcgcgt ttcgtggcgg
     2281 aacgaaatgc ggtcatagtt agaatcctcg atctgaattt cggcgcgccc ggcttttctc
     2341 cattcgacgc aatggccttg aacccgacgc cgccaagcgc ctagtgtccc gattccgaaa
     2401 ttcgcaccct ctctatatcg gcttcgggcg aatttcggaa tcgaaaggac gctagcaacc
     2461 tattgaatcc tagtgtcgtt ttggtttcaa agttcgcttg cggcccgata gtgcgcatgg
     2521 tgcgaactta gaaaccacga cactagaaga atgcacattt ccaggaccgg ttccagtccg
     2581 tgaccggcag gagaaacagg atgaaccttt cgcgctttgt cgccccgtct actttcgcgc
     2641 tgatcgcggc ggcgatcgtt gcccaagcgg cgccggtccg cccgccgatc accagtgttt
     2701 cgcatctgag cgtgtacagc gccgacgccg ccaagacgga gtatttctac gtccacaatc
     2761 tgggcgcggt gaaacgcgac gatccggaaa atgccaaagg tgtgcgctac tatttcaacg
     2821 cgctgcaatt catcgaagtg ctgccgctgc ccgaggcgga ggccaagtcc gccaaccgga
     2881 tggaccatgt ggcctacaac acgcccgacg tgaaggcgat gaagcgctat ctcgccgcgc
     2941 acggcgtcaa ggcgcaggac gccatccgca aaggggccga cggcagccaa tggttcgacg
     3001 tcagcgatcc tgaaggcaac attgttcagt tcgtgcagcc gccgaaggcg ccgcagccag
     3061 ttgccgacag cccgctcagc aaccacatca tccatgtcgg cttcatcgtc cacgaccgga
     3121 cgctggaaga cggcttctat ctcaagctgc tcggcttccg tccctattgg tacggcggca
     3181 aggagggcga aaagccggcc tggatctcgg cgcaggtccc cgacggcacc gactggatgg
     3241 aatatatgat ggtcagcggc ccgcagacgc gcggcatccc ggcggccatg tcgcaaagca
     3301 cggcgggcgt tctcaaccac ttctcgctcg gcgtggcgaa catggaaaaa acggtgacgc
     3361 tgctgaggga aggcggccgt atctcggaaa agaacgacgg cccgaaaatc ggcgccgacg
     3421 gcaagtggca gtttaacacc tatgaccccg acggcacacg cgccgaattc atggaattcc
     3481 aagccgtggt caagccttgc tgctcgccgt tcaccgccag cgatccggag aagtaaccgg
     3541 ccagccggca cgcaaagtcc gccttgggcg gctacgtgga taagcgcgag gtgcgcgccg
     3601 gtgtcggcat gcgcgtccac tatggcgttg accagcgccc aggtccattt gcagcgctcg
     3661 acatgggcgc ggatcgcggc gcggcgggcc cgcgccttcg cggtttttgc cccagttcgg
     3721 attttgcctg ctggcccggc ggcttgcgcg gggttcggca agataattgt tccctgcgcg
     3781 cggcagcccc ctcccccatc ccaaggttct ttgacgtgag ccacccttcg acaagctcag
     3841 ggtgaaggta tcattcgaac tttcatgctg aaccccgcat cccgagcttg tcgagggacg
     3901 aaacatgcgg cgcggacggc gcgcgtatgc cgccgcgacg atgcggggcg tttgcgacgg
     3961 agaaagagcg cctgccttca tgggcgcatt ttacgcccga ggcggagagt cttggataag
     4021 ccggacggcg gcgaaagaag cgcgtgatcc ggttgaagaa aacggcgcgg acgaagaggg
     4081 atagggttca atggccgcag tgcccgccca acgctcatct gtgctaatct gctcggcgga
     4141 ttggcgggga taattccgcg caaccaaggg gggaaccatg ttgcgattgc gtattgcggt
     4201 catggtgctt ggcgccatgg cgatcctgac gacatctgct ccggcgcaag atgccggcag
     4261 tctcgaaacg gtcgtggtca cgggggcgcg cgcggaatac gacgcgcaga ccgtgcctca
     4321 tgtgttcctg ctgcgccggg ccgaccacct catcaccaaa gtgcgcgtgg tctgcgatac
     4381 ccgcgagctg agcaagcgcc ggcaagagct gaaggacacg ctgcgcaaca tgatccgcga
     4441 ggcgtcgaag agcggcacca tctctctggg cgtcggcgac gaaatcgtcg cagacctgac
     4501 ggagaagatg ctcgacaagg ttatcgcgcc cgacgcgaga gcggatacca gcatcgcagc
     4561 tgtcgtcgtc aaaacccgtg tcacgaagga agatgcgttc gacgatgcaa ccgggcggat
     4621 cgaacggttc atcgaggcca cgcccaaatc cgggcgcacc gaaatcctgc gcgtgaaccg
     4681 ctgggacttg acgatcgtcg ggccggaaca ataccgcgcc gcgctgatcg ccaaagtggc
     4741 caaagacgcc aagcagacgg cggcgctgtt cggagcggac tatgcggtcc gcatcgaggg
     4801 cctgcagcat cccattgcct ggtatcaaaa ggggccgctc gacctggcgc tatacattcc
     4861 gtacacgctc accatcgcgc cgccggtccg gtgaccgggc ggccccggcg aaccgggcgg
     4921 cgcgttcgcg cgcctatttc gcccattgcg ccagccagtc ggcgatggct tgcggcgtca
     4981 tgtggcgcgc gtcggccagc gcggcgatgc ggcccggatt gacgaatttt ccattcggct
     5041 cggcgatgat gaccgaaggc acgccttcca atctcttggt gatgccgaag cgcgccggga
     5101 tttgcaggtt cttgtcgaag cggccgacat cgacggcgac gatctcgaaa tgcgccgcca
     5161 gaaaaggttt catttcgggt agctgcatca gattggcgag cacgatgcag tctgggcacc
     5221 agttgccgcc caggtcgatc agcacccgct tgccgttctt cttggcgcgg gcaaaggccg
     5281 catccaccgc ggcggtggca ttggcattgg gattataggg cgagcgctcg accaccggca
     5341 gatcggccag cgaagcgata tggaccttgg gcgccgtggc ggcgattgaa gcgccagcaa
     5401 gaaacggcaa gaccgacagc gccatgagca atgttcttag cttcatacga actctcccta
     5461 cgtttccggc ctttttggcg atgcgccgcg gtttgcgaaa ggacggactg aagctatcct
     5521 agcggcggcc gtctccttcg cgcaacaagg cgcgagaagt tgaacgcttt cgcttggtct
     5581 tttgctgtaa gacggcctgt gcatcatcag gggaaactca tgaaacgaat tgtgcttgtc
     5641 gccgctctgg cattcttgcc ggcgctggct cccgcttatg ccgcggatcc cgtccagccc
     5701 gccatgatgt gggacttggg cgatctttac gcctcgcccg aagcctggag cgcggcccac
     5761 gccaaagcct tggccgaagc ccagaagctc gacggctaca aagggacgct ggccaac
//
LOCUS       DASWPU010000611         7348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_29476,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000611 DASWPU010000000
VERSION     DASWPU010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29476"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..680
                     /locus_tag="VGC27_13035"
     CDS             72..680
                     /locus_tag="VGC27_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283535.1"
                     /translation="MPLRHEAVDKTKFNKKAVLPYQLRLTDFESAMQDAYDFFHDVNE
                     LLLNRGLHRLDDMLRPAAMSGIISDMLTASMAKHARSLVENNHFNGHPDLIVQGHYAN
                     NSVKAGKDGVEIKSTRKKGGAVDTHGARNQWMCVFVYETDSATEPAMARAPMRFIQVY
                     LGQVTQTDFRRNARGELGTRTATLDRAGIQKLRANWVYLLER"
     gene            complement(645..1775)
                     /locus_tag="VGC27_13040"
     CDS             complement(645..1775)
                     /locus_tag="VGC27_13040"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY0283536.1"
                     /translation="MNHRTAPGLVRDSIVTYLAAAESASIAEICEAVASRIGDVPASS
                     VRSYLNIKTPELFERTERGHYRLRKANVRAAGVPLEPKYTIGRAKLYHANCFDWMRAA
                     ELHSIHAIVTDPPYGLLEYTETEQAKLRKGRGGVWRIPPSYDGHQRAPLPRFTVLNDG
                     DLSVMRDFFFEFGQLSLRVTVPGANILIASNPLLIHVVANAMSDAGLEVRGQLVRLVM
                     TMRGGDRPKNAHEEFPHVSVMPRSMFEPWLVLRHPLDGRAQDTLRKWNTGGFRRLSAE
                     RPFGDVIASQPTHKSEKLIAPHPSLKPQAFLRAIVAAVLPMGKCVILDPFAGSGSTLA
                     AANVLGIESIGIEKDARYVGMAQKAIPKLAALSLKKIDPIGA"
     gene            1972..3663
                     /locus_tag="VGC27_13045"
     CDS             1972..3663
                     /locus_tag="VGC27_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY0283537.1"
                     /translation="MAHSFDRRGVLKIGAATVAAPYLWLPARAEEPAWPTQTVGPFVE
                     VEINSGRIRGGHSRGALAFKNIPYAGPVSGKNRFKAPPKVTPWAGVRDATRLGPPALQ
                     SPGSTYGEHEPAYSEDCLVLNVWTPAVNDGGKRPVMFYSHGGGFTSGSGGQNIQDGAH
                     LAVTYDVVVVASNHRLGLFGYLYLGELGGEEYATSGNQGMLDIAAALAWVKENIATFG
                     GDPVNVMAFGESGGGSKTCALMAMPAAHGLFHKAGIQSGPMLQGTTKEAATETARRVL
                     AGLGIAPQDASKLAEVPAEKLLAIQLAGGKGPLSAPTKDWLSRHPKPPTAAQAEGHDQ
                     PGNWGPVVDGAVLPQNPFDPAAPVISAEIPLLIGNNRDEATFFMRDRPDFFHGGEAAL
                     TAMAHQLLGDAADRVVALYRRTRPHATPQELGIAILTAVTFGNGTVLVADRKAAQPAP
                     VYRYRYDFESNTPIEGTDWTLRAGHASDISLVFYNYEFKDLQGQGPGLAEASKAMSGY
                     FTGFARSGVPSAEGQPQWPRYDTNTRAVMLLNSQCRVVNDPDSEERKFWQSLGKG"
     gene            complement(3677..4144)
                     /locus_tag="VGC27_13050"
     CDS             complement(3677..4144)
                     /locus_tag="VGC27_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY0283538.1"
                     /translation="MPDIPSSVAEFLAGKRFAVAGVSRQPQQAANAIYRRLVNSGFEV
                     LPVNPNAAEVEGVRCYPDLRSIPGAIDGVVVATHPRAALDIVRQCADKGIRRVWFHRS
                     FGAGSVSKEAVKACEESGIHCIAGGCPLMYCEPVDAGHRCIRWWLRLFGKVPG"
     gene            complement(4235..4810)
                     /locus_tag="VGC27_13055"
     CDS             complement(4235..4810)
                     /locus_tag="VGC27_13055"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY0283539.1"
                     /translation="MTRLSPDERARLRAACEAAEKRTSARFELVTVPVSDRYTLYPVV
                     YGAIAGLTALGALALFWPELSLRMGFLAAAALFAAASFLFGWLPLRLMLVPRYAKSWE
                     CWELAHRSFASRILAQTDRKTGILLFVSLGEHYVEVVTDRDVDLKVLQSTWDAIIKDF
                     TAAAKEKRLADGLVAAVDACAKVLGTHYPAK"
     gene            complement(4807..5553)
                     /locus_tag="VGC27_13060"
     CDS             complement(4807..5553)
                     /locus_tag="VGC27_13060"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY0283540.1"
                     /translation="MTKMARSFAHAFLVALIGVVVAVGAAGAAPSFPPLTGRVVDEAG
                     ILSQAAQGRLTALLAEHERQTGNQIVVATVKSLQGEDIAEYGVQLGRAWGIGQKGKNT
                     GALVIVSPSSRDVRIEVGYGLEGDLTDAQSKLIIENTMLPFFKKGDYDSGLLAGTVQI
                     LQALGGKPTGAESIPQPESQPDGAGAIPIFVLLIILWAVFGRFFWPLFFLGGIGRGGG
                     FGGGSGGGFGGGGFSGGGGSFGGGGASGRW"
     gene            complement(5553..6155)
                     /locus_tag="VGC27_13065"
     CDS             complement(5553..6155)
                     /locus_tag="VGC27_13065"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY0283541.1"
                     /translation="MSNGFTRIAGRIAVLGVLGFALAGCGINKIPAQDEQVKAAWSQV
                     QNQYQRRADLVPALVATVKGYAKQELTVLTQVTEARAKASQTVLPDDILSDPKAFQQF
                     EQNQAQLGGALSRLLVTVEAYPQLKSDKNFLTLQAQLEGTENRIAVARRDYILAVQSY
                     NTLLRTIPTRWTAAIFYPELKPYQTFATSEKNQSAPQVQF"
     gene            complement(6536..>7348)
                     /locus_tag="VGC27_13070"
     CDS             complement(6536..>7348)
                     /locus_tag="VGC27_13070"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0283542.1"
                     /translation="QSAVSRQISALEDEITTPLFQRHARGLTLTDEGEMLYAAVSDVL
                     TRLAQAEEALKNVREAPRGTLKVTASNGIATYWLVPRLHKFLEECPEVEFHLVIEDRE
                     LDLAQREADIAVRMRAPVQADLIQRKLLTVHYHIYATREYLDANGTPDCFDDLARHTI
                     IAYGETAAPEIREVNWLIEQTRRLGRPAGKGRTVRINNVTGILRAAQSSLGIAAMPDY
                     VAAEYPDLIRVLPEIPGPSFDVHLVYADALRQSKRVAAFRDFLVREAKDWHY"
BASE COUNT         1393 a         2294 c         2342 g         1319 t
ORIGIN      
        1 ggttacgatc tatataacat cctgaccgat tcggctgata tgctgagaaa aaggaaactg
       61 ggttccccgc cgtgcccctt cgccacgaag ccgtagataa aaccaaattc aacaagaagg
      121 cggtactgcc gtatcagctc cggcttaccg acttcgagtc ggcaatgcag gacgcttatg
      181 attttttcca tgacgtgaac gagttgttac ttaaccgagg cctgcaccgg ttagacgata
      241 tgctgcggcc ggctgcgatg tcaggaatca tctccgacat gctgacggcc tcaatggcga
      301 agcatgcacg atccctcgtt gaaaataatc atttcaacgg gcatcccgat ttgatcgtgc
      361 aaggtcatta cgcgaacaac tccgttaagg ccggtaagga cggcgttgag atcaagagca
      421 cgcgaaagaa aggtggagcg gtcgatactc acggcgcgcg taatcaatgg atgtgtgttt
      481 tcgtctacga gacggacagc gctaccgagc cagcaatggc acgcgcgccg atgcgcttca
      541 tccaggtata cttggggcaa gtgacccaaa ctgatttccg gaggaacgcc cgcggcgaat
      601 tggggacgcg cacagcgact cttgatcgcg ccggcattca gaagttacgc gccaattggg
      661 tctatcttct tgagagatag cgcggcgagt ttcggaatag ccttctgggc catgccgaca
      721 tagcgcgcat ctttttcgat gccaatgctt tcaatgccaa gcacattggc tgcggccaga
      781 gtcgaaccgg aaccagcgaa cgggtcgagt atcacacact ttcccattgg cagcactgcg
      841 gccactatgg cgcgcaagaa agcctgtggt tttaaggaag gatggggtgc aatcagcttt
      901 tcacttttgt gtgtcggttg cgacgcgatg acgtcaccaa acgggcgctc ggccgagaga
      961 cgacgaaatc ctcccgtatt ccacttgcga agcgtgtcct gcgcgcggcc gtcgagcggg
     1021 tggcgcagca ctagccacgg ctcaaacatc gaacggggca tgacactgac atggggaaat
     1081 tcctcgtgtg cgttctttgg cctgtcaccg ccgcgcattg tcatgacaag gcgcacgagc
     1141 tggccgcgca cttcaagtcc agcgtcgctc attgcgtttg cgacgacgtg aatcagaagc
     1201 ggattactcg caataaggat gtttgcaccg ggtactgtca cgcgcaggga gagttgaccg
     1261 aactcgaaaa agaaatcgcg cataaccgag agatcgccgt cattcaaaac agtaaagcgg
     1321 ggcagaggcg cacgctgatg tccgtcgtat gagggcggta ttcgccacac gccgccacgt
     1381 ccctttcgga gctttgcttg ctctgtctcg gtgtactcga gcaacccgta gggcgggtct
     1441 gtgacgatcg cgtgaatgct gtgcaactcc gccgcccgca tccaatcgaa gcagtttgca
     1501 tgatagagct ttgcccggcc gatagtgtat ttcggctcca acggaacacc ggcggcgcgg
     1561 acattggcct tgcgaagcct ataatgtccg cgctctgtgc gctcgaatag ttccggcgtc
     1621 ttgatgttaa gatatgaacg aaccgacgag gcggggacgt ctccgatgcg cgacgctacc
     1681 gcctcacaga tttcagcaat tgaggcgctt tcggcggcgg ccagataggt cacgatggag
     1741 tcgcggacga ggccgggtgc ggtgcgatga ttcattgttt cctcgaatga aagaataggt
     1801 ttcagacgtc tagacgtcaa tacaaaagag acggcggaca gccgccggcg atgcagtggt
     1861 gccgctttcc ttgcacgcct tgatcgcttg cttcgacacg ccgccggtgg tgataatccg
     1921 ctctattgcc tgcaaagcgc gcatcaccaa gaataaatca gggaggagat cgtggcacat
     1981 tcctttgacc gtcgcggcgt tttgaaaatt ggcgcggcga cggtcgccgc accttatctg
     2041 tggctgccgg cgcgcgccga agaacccgcc tggcccaccc agacggtcgg ccccttcgtc
     2101 gaggtcgaga tcaacagcgg gcgcatccgc ggcggccaca gccgcggcgc gctggccttc
     2161 aagaacattc cctatgccgg cccggtgtct ggcaagaacc gcttcaaggc gccgcccaag
     2221 gtaacgccgt gggcgggcgt gcgcgacgcc acccgcctcg gcccgccggc gctacagtcg
     2281 ccgggtagca cgtacggcga gcacgagccc gcctacagcg aagattgcct ggtgctcaat
     2341 gtctggaccc cggcggtgaa cgacggcggc aagcggccgg tgatgttcta tagccatggc
     2401 ggcgggttca cctccggctc cggcggccag aacatccagg acggcgcgca tctcgctgtg
     2461 acctatgacg tggtggtggt ggcgagcaat caccggctcg gcttgttcgg ctatctctat
     2521 ctcggcgaac tgggcggaga agaatacgcc acatcgggca accagggcat gctcgacatc
     2581 gccgccgcgc ttgcctgggt gaaagagaat atcgccactt tcggcggcga tcccgtcaat
     2641 gtgatggcgt tcggcgagtc cggcggcggc tccaagacct gcgcgctgat ggccatgccc
     2701 gccgcgcacg ggctgttcca caaggctggg attcaaagcg gccccatgct gcaaggcacc
     2761 acgaaggagg ccgccaccga aaccgcgcgc cgcgtgctgg cgggcctcgg catcgcaccg
     2821 caggacgcaa gcaagctggc cgaggttccg gcggaaaagc ttctcgccat acagcttgcg
     2881 ggcggcaagg gaccgctgtc ggcgccgacc aaggattggc tttcccggca ccccaaaccg
     2941 cccaccgccg cacaagcgga aggccacgat cagccgggca attggggccc ggtggtggac
     3001 ggcgctgtcc tgccgcagaa tcccttcgat cccgcggcgc ccgtgatttc ggccgagatt
     3061 ccgcttctga tcggcaataa ccgcgacgag gccaccttct tcatgcgcga ccgtccggat
     3121 ttcttccacg gcggcgaggc ggcgctgacg gcgatggcgc atcaactcct cggcgacgcg
     3181 gccgaccgcg tcgttgcgct ttaccgccgg acaaggccgc acgcgacgcc gcaagaactc
     3241 ggcatcgcca tcctgaccgc ggtcactttc ggcaacggca cggtgttggt ggccgaccgc
     3301 aaagcggcgc agcctgcgcc ggtctatcgc taccgctacg atttcgaatc gaatacgccg
     3361 atcgagggca cggattggac cctccgcgcc ggccatgcca gcgacatctc cctggtgttc
     3421 tacaattacg aattcaagga cctgcagggc caaggccccg gcttggccga ggcttcgaag
     3481 gcgatgagcg gctatttcac cggcttcgcg cggagcggtg ttccgtccgc cgaagggcag
     3541 cctcaatggc cgcgctacga taccaacacg cgcgcggtga tgctgctcaa cagtcaatgt
     3601 cgggtggtga acgacccgga tagcgaagaa cgcaaatttt ggcagtctct cggcaagggg
     3661 tgagatcggc ttacccctat cccggcactt tgccgaacag gcgcagccac cagcggatgc
     3721 agcgatggcc cgcgtccacc ggctcgcaat acatcagcgg gcagccgccc gcgatgcagt
     3781 ggatgccgct ctcttcgcac gccttgaccg cttctttcga cacgctgccg gcgccgaagg
     3841 agcggtggaa ccatacgcgg cgaatgccct tgtcggcgca ctggcgaacg atatccagcg
     3901 ccgcgcgcgg atgggtggcg accaccacgc cgtcgatggc tccggggatc gatctgagat
     3961 cgggatagca gcgcacgcct tcgacctcgg ccgcgttcgg attgaccggc agcacctcga
     4021 agccggagtt gaccagtctg cgatagatgg cgttcgccgc ttgctggggc tgcctggaaa
     4081 cgcccgcgac ggcgaagcgc ttgccggcga gaaattcggc gacgctggat ggaatgtctg
     4141 gcatggatga tccccccttc gtcccccttc gcttgcttcg caagcttcgg aggacacttc
     4201 ccccgctaaa gcgggggcag aaatcaaatc gccctcactt cgccgggtaa tgcgtcccca
     4261 gcaccttggc gcaggcgtcc accgccgcga ccaagccgtc ggccaggcgc ttctctttcg
     4321 cggcggcggt gaagtccttg atgatcgcgt cccaggtcga ttgcagcacc ttgagatcga
     4381 cgtcgcggtc ggtcaccact tccacataat gctcacccag cgagacgaac agcaggatgc
     4441 cggtcttacg gtcggtctgg gccagaatgc gcgaagcgaa ggaacggtgg gccagctccc
     4501 agcattccca gctcttggcg tagcgcggca ccagcatcag gcgcagcggc agccatccga
     4561 acaggaacga cgccgccgca aacagggcgg ccgcggcgag aaatcccatg cgcaatgaaa
     4621 gttccggcca gaacagcgcc agggcgccga gagcggtcaa tccggcgatc gcgccgtaga
     4681 ccaccggata aagcgtgtag cggtcgctga cgggaaccgt gaccagttcg aaacgcgcgc
     4741 tggtgcgctt ctcggcggct tcgcaggctg cacgcagccg ggcgcgttcg tcaggggaaa
     4801 gacgcgtcac caacgtcccg atgcgcctcc gccgccgaaa gaaccgccgc cgcccgagaa
     4861 tccgccgccg ccgaagccac caccggagcc gccgccaaac ccgccgccgc gcccgatgcc
     4921 gccgaggaag aacagcggcc agaagaagcg cccgaacacc gcccacagga tgatgaggag
     4981 cacgaagatc ggaatggcgc cggcgccgtc cggctggctc tcgggttgcg ggatcgattc
     5041 cgcgcccgtc ggcttgccgc ccagcgcttg cagaatctgc accgtgccgg cgagcaggcc
     5101 ggaatcgtaa tcgcccttct tgaagaaggg cagcatggtg ttttcgatga tgagcttgga
     5161 ttgcgcgtcg gtgagatcgc cttccaggcc gtagccgacc tcgatgcgca cgtcgcggct
     5221 gctcggcgag acgatcacca gcgcgccggt gttcttgcct ttctggccga taccccaggc
     5281 gcggccgagc tgaacgccgt attccgcgat gtcctcgcct tgcagcgatt tgaccgtggc
     5341 gacgacgatc tggttgccgg tctgccgctc gtgctcggcg agcagggcgg tgaggcggcc
     5401 ctgcgccgcc tgcgaaagaa tcccggcttc atcgacgacg cgcccggtga gcggcggaaa
     5461 gctgggggcg gccccggccg cgccgaccgc cacaacgacg ccgatcagcg caacgaggaa
     5521 ggcgtgcgcg aaggatcgcg ccatcttggt catcagaact gaacctgcgg ggcggattgg
     5581 ttcttctcgc tggtggcgaa ggtctggtac ggcttcaatt cggggtagaa gatcgcggcc
     5641 gtccagcggg tgggaatggt gcgcagcaaa gtattgtagc tctgcacggc aagaatgtaa
     5701 tcgcgccgcg ccacggcgat gcggttctcg gtgccttcaa gctgcgcctg caaggtcagg
     5761 aagttcttat cggacttaag ctgcggatag gcttcgaccg tcaccagcag ccggctcaac
     5821 gcgccgccca actgcgcctg gttctgttcg aactgctgga acgccttcgg atcggaaaga
     5881 atatcgtcgg gcaggaccgt ctgcgacgcc ttggcgcgcg cttcggtcac ctgcgtcagc
     5941 acggtgagtt cctgcttggc atagcctttc acggttgcga cgagagcggg cacaaggtcg
     6001 gcccggcgct ggtactggtt ctggacctga ctccaggcgg ctttcacctg ctcgtcctgc
     6061 gcgggaatct tgttgatgcc gcagccggcc agggcaaaac ccagcacgcc caggaccgca
     6121 atacggcccg cgattctcgt aaacccattc gacatggcaa tacctcccgg attggccgaa
     6181 ccgcgtccgc ggctctttgt accacgcctg cctgttcggc cggatacggt taagcaggcg
     6241 ctcccaaaaa aaagacgccg gagcgggggg gcgctccggc gtccagcttg ggatgcccgc
     6301 gtgtgttggg gcgcgagccg agccgggcag cgcgtgcgcc atccacggct ctcgcgaagg
     6361 ccacttcgcg acggcagacc tggggggagg cgctgccgta ttatttagat agggcgcgtt
     6421 tttagacttt caataggtgg aaaaccccgg ctgtcctcat cctgaggagc cctttcgccc
     6481 gtccgcacgc tgtcgctacc ggccttcaat gagggcgaaa gggcgtctcg aaggatcaat
     6541 agtgccagtc tttggcttcc cggaccagaa aatcgcggaa tgccgccacg cgcttggact
     6601 ggcgcagggc gtcggcatag accagatgga cgtcgaagct ggggccgggg atttccggca
     6661 ggacgcggat gagatcgggg tattcggcgg cgacataatc gggcatggcg gcgatgccca
     6721 ggctcgattg cgccgccctt aggattccgg tcacgttgtt gatacggacg gtgcggccct
     6781 tgccggccgg ccggcccagc cggcgcgtct gttcgatcag ccagttgacc tcgcggattt
     6841 ccggcgcggc ggtctcgcca taagcgatga tcgtgtgccg ggcgaggtcg tcgaagcaat
     6901 ccggcgtgcc gttggcgtcc agatactccc gtgtcgcgta aatatgataa tgcacggtca
     6961 gaagcttgcg ctggatcagg tcggcctgca ccggcgcgcg catgcgaacg gcgatatcgg
     7021 cttcgcgctg ggcgagatca agttcgcggt cttcgatcac cagatggaat tcgacctcgg
     7081 ggcactcttc caggaatttg tgcaggcgcg gcaccagcca ataggtggca atgccgttgc
     7141 tggcggtgac cttcaaggtg ccgcgcggcg cctcgcgcac gttcttgagc gcttcttcgg
     7201 cctgcgccag acgggtcagc acgtcgctca ccgcggcgta gagcatctcg ccttcgtcgg
     7261 tcagcgtcag gccgcgggcg tggcgctgga acaaaggcgt ggtgatctcg tcttcgaggg
     7321 cgctgatctg ccggctcacc gccgactg
//
LOCUS       DASWPU010000612         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_36562,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000612 DASWPU010000000
VERSION     DASWPU010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36562"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGC27_13075"
     CDS             complement(<1..267)
                     /locus_tag="VGC27_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283543.1"
                     /translation="MSSRPLLVFSLLAALTLACNKVPENTVVAKRQATPQENRKFEQL
                     ALEARGAFLKEPRDIGTVETSARKYAEATAIRANDYVVMWEAARS"
     gene            418..1140
                     /locus_tag="VGC27_13080"
     CDS             418..1140
                     /locus_tag="VGC27_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283544.1"
                     /translation="MATAPIAPETAPAGSHRRIFESRYRALPQEAREQTAATASEPDL
                     SALCYDPVPAVIRGILDNPLAGLAHARLIAAHHPNPIGLQAVAARLVFAQDREVQRLF
                     LRNIQTPVSVVRSVFGSRRLTDIFNFSRSHDLPDRHRETARATLKQRFLTAPPEERAD
                     LIVATEGRTLTMLSGLPLDGPTVSLLCARGTLSMPLIENLARWPATPPKLIVHLLKQP
                     LVGQFPALKQAVRRHPNCPSNA"
     gene            1221..1877
                     /locus_tag="VGC27_13085"
     CDS             1221..1877
                     /locus_tag="VGC27_13085"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0283545.1"
                     /translation="MLRINDEAPNFTAESSKGTINFHDWLGNGWGILFSHPKDFTPVC
                     TTELGYMAGLQPEFEKRNCKIIGLSVDQVSNHSKWSVDIEETQGHKVNYPMIGDPELK
                     VAKLYGMLPAETGETCEGRTAADNATVRTVFMVGPDKKIKLMLSYPMSTGRNFDEVLR
                     VLDSMQLTAKHKVATPVNWKQGDDVIIAGSVNEEDAKKQYPNGWKAPKPYLRIVPQPK
                     "
BASE COUNT          377 a          630 c          606 g          326 t
ORIGIN      
        1 cgagcgcgca gcctcccaca tcacgacgta gtcgttcgcg cgaatggccg tggcctccgc
       61 gtatttccgc gcactggttt ccacggtgcc gatgtcgcgc ggctccttga gaaaggcacc
      121 gcgagcctcg agcgcgagtt gctcgaactt gcgattttcc tggggggtgg cctggcgctt
      181 cgcgaccacc gtgttctccg gcaccttgtt gcaagccaag gtaagggccg cgagcagcga
      241 gaagacgagc aggggacggg atgacatcac agtggccctc ctgggaaaga gacccagaaa
      301 gacgatgatg cggggggggc cctaggcggc gccatggggt attgaccccg gtcggggttg
      361 cgcggcggga ctccagtcac caagtttgcc ctggttccgg actcactcga ggatcagatg
      421 gcaactgccc ccattgcgcc cgaaaccgca ccggccgggt cccaccggcg gatcttcgag
      481 tctcggtacc gcgcgcttcc gcaagaagct cgcgagcaaa cggccgccac cgcgtccgaa
      541 ccggacctct ccgcgctctg ctatgacccg gtccccgcgg tgattcgtgg aattctggat
      601 aacccgctgg caggactcgc gcacgcgcga ctgatcgcgg cgcaccaccc gaatccgatt
      661 gggcttcagg cggtcgccgc gcgcctcgta tttgcgcaag atcgcgaggt gcagcgcttg
      721 tttctccgca acatccagac cccggtgtcc gtggttcgaa gcgtgttcgg ctcgcgacgg
      781 ctgaccgaca ttttcaattt cagccggagc catgacttgc ccgatcgcca ccgtgagacc
      841 gcgcgcgcca cgctgaagca gaggttcctg acggcaccgc cggaagagcg agccgacctg
      901 atcgtcgcaa ccgaaggacg cacgctcacg atgctctccg gactcccgct ggacggaccc
      961 accgtgtccc tcctctgcgc acgcggcacg ttgtccatgc ctctcatcga gaacctggct
     1021 cgctggcccg cgacgccgcc gaagttgatc gtgcatctgc tcaagcagcc gctcgtcggc
     1081 cagttccccg cgctgaaaca ggccgtgagg cgtcacccga actgcccctc caacgcgtaa
     1141 tcctcgctga aaccgcgcct tccgcgtggt aaactcgccg ccacttttcg cgagttgaac
     1201 acactggaag gagacgggtt atgttgcgca tcaacgacga agcgccgaac ttcacggcgg
     1261 aatcctcgaa gggcacgatc aactttcacg attggctcgg caacggctgg ggcattttgt
     1321 tctcgcaccc caaggatttc acgccggtct gcacgacgga gctgggctac atggccgggc
     1381 tacagcccga attcgaaaag cgcaattgca agatcatcgg gctgagcgtg gatcaagtga
     1441 gcaaccacag caagtggagc gtcgacatcg aagagaccca gggccacaag gtgaactacc
     1501 cgatgatcgg cgaccccgag ctgaaggtcg ccaagctcta cggcatgctg ccggccgaga
     1561 cgggcgagac ctgcgagggg cgcacggccg ccgacaacgc gacggtccgt acggtgttca
     1621 tggtgggacc cgacaagaag atcaagttga tgctcagcta cccgatgagc acgggacgca
     1681 atttcgacga ggtgttgcgc gtgctcgact cgatgcagct caccgccaag cacaaggtcg
     1741 cgacgccggt gaactggaag cagggcgacg acgtgatcat cgcgggctcc gtgaacgaag
     1801 aggacgcgaa gaagcagtac ccgaacggct ggaaggcgcc gaagccctac ctccgcatcg
     1861 tgccgcagcc gaagtagttt tttctcgtgc agtggaagca ggccgtgtcc cccgaagggc
     1921 gcggcctgtt tcatttgat
//
LOCUS       DASWPU010000613         4177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_5137, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000613 DASWPU010000000
VERSION     DASWPU010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5137"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VGC27_13090"
     CDS             <1..594
                     /locus_tag="VGC27_13090"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283546.1"
                     /translation="LSPEDAPDKPTIIEIGFDKGDAVSIDGAALSPASLLTRLNELGK
                     ANGIGRVDLVENRFVGMKSRGVYETPGGTILLAAHRAMESITLDRGAAHLKDELMPRY
                     AELVYYGFWFAPEREMLQAAIDKSQEFVAGSVRLKLYKGNVIVIGRASPYSLYSKDLV
                     TFEDDQGAYDQKDAAGFIKLNALRLRTLAMRKRKMKR"
     gene            complement(821..3283)
                     /gene="gyrB"
                     /locus_tag="VGC27_13095"
     CDS             complement(821..3283)
                     /gene="gyrB"
                     /locus_tag="VGC27_13095"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY0283547.1"
                     /translation="MSEAAKIHAAELAAQQASQQASDDYGADSIKVLKGLDAVRKRPG
                     MYIGDTDDGSGLHHMVYEVVDNAIDEALGGFADRIDVILNPDGSCTVRDNGRGVPTDI
                     HSEEGVSAAEVIMTQLHAGGKFEQSVYKVSGGLHGVGVSVVNALSERLDLRVWRDGKE
                     HYMQFRHGEPQAPLKVVAEVKNKRGTEVTFLPSPQTFTMTEFDYPTLEHRLRELAFLN
                     SGVTIFLADKRGVEPKESKLHYEGGLEQFVRYLDRAKNSLIPNPINIIAEREGITVEA
                     ALTWNDSYHESMLVFTNNIPQRDGGTHLAGFRAALTRVVTKYADESMGAKKDKTALAG
                     DDCREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPVVEGVVLEKLSGWFEEHPAEAKA
                     IVGKVIEAAAAREAARRARELTRRKGALDGASLPGKLADCQERDPAKCELFLVEGESA
                     GGSAKQARNRANQAVLPLRGKILNVERARFDKMLASQEIATLITALGTGIGSEEFNPD
                     KLRYHKIIIMTDADVDGAHIRTLLLTFFYRQMPALIERGHLFIAQPPLYKAARGKSER
                     YLKDENELQDHLIAEGTAGASLTLHTGEVVTGADLAALVEKARVAVTALEGFPRHYPR
                     FVLEQAAIAGALNPEILGDAAKATEAAAYIARRLDLLSDETERGWHGEPTADGGLKFW
                     RDVRGVREAVAIDGALIASTDARKLDRMAPDLQAAYLKFGVVARKDESREVRAPSELL
                     GAIFEWGRKGLTLQRYKGLGEMNPQQLWETTLDDNARTLLRVKVEHADEADDLFAKLM
                     GEIVEPRREFIQDNAMNAALDV"
     gene            complement(3293..3901)
                     /locus_tag="VGC27_13100"
     CDS             complement(3293..3901)
                     /locus_tag="VGC27_13100"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY0283548.1"
                     /translation="MLSWHAYLLYCGLYAIAIAVPGPGIVAIVARALGSGFRATIPAA
                     IGTAAGDLILMSLSAFGLALVAQAMGGLFLIVKLMGAAYLVYLGYKYWTAPVADLETV
                     VPASARQGFLAQMALTLGNPKAIAFFVALLPTVVDLGRLNAFGYLQLCAATFVLIPAI
                     TLSYAALASRVRGFLASRRARGRLNKGAAVIMAGAGAGVAVS"
     gene            complement(3906..>4177)
                     /locus_tag="VGC27_13105"
     CDS             complement(3906..>4177)
                     /locus_tag="VGC27_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283549.1"
                     /translation="TGEQKALLVSIVLADAWELTRARGGIAPLLLLDEIAAHLDARRR
                     AALFEELFALGTQAWMTGTDLSLFDSLSSRADVFTVENGRFVRQE"
BASE COUNT          696 a         1342 c         1381 g          758 t
ORIGIN      
        1 ctgtcgccgg aggacgcgcc cgacaagccg acgatcatcg aaatcggttt cgacaagggc
       61 gacgcggtgt cgatcgacgg tgcggcgctg tcgcccgcgt cgctgctgac caggctgaac
      121 gagttgggca aggcaaacgg catcgggcgc gtcgatctgg tggagaatcg cttcgtcggc
      181 atgaaatcgc gcggggtgta cgaaacgccg ggcggcacga tcctgctcgc cgcccatcgc
      241 gcgatggaat ccatcacgct ggatcgcggc gcggcgcatc tgaaagacga gctgatgccg
      301 cgctacgccg agctggtcta ttacggtttc tggttcgcgc ccgagcgcga gatgctgcaa
      361 gccgccatcg acaagtcgca ggagttcgtc gccggctcgg tgcggctcaa gctctacaag
      421 ggcaacgtca tcgtcatcgg ccgcgcctcg ccttattcgc tctattccaa ggaccttgtc
      481 accttcgagg acgaccaggg cgcttacgac cagaaagacg cggccggctt catcaagctc
      541 aacgccctgc gccttcgcac cctggcgatg cggaagagga agatgaaacg ttgattgtca
      601 tggcccgcga gttcgtagcg acagcgtgcg aacgggccac ccaggtgacg ttgctcgaat
      661 ttcttccttt cgtcctggcc gccacaaggg cgaccatcaa atgaaatggc aagatcacgc
      721 ggaggcgcgg agtggaggcc cattgcgcgc ctccgcgtac aaattcggat gtcgttctta
      781 cgctgccgct aacgatcaag tgcgtccatg acgagaacgg tcacacatcc agcgccgcgt
      841 tcatggcgtt gtcctgaatg aactcgcggc gcggctccac gatctcgccc atcagcttgg
      901 cgaacagatc gtcggcttcg tcggcgtgct ccactttcac ccgcagcagc gtacgggcgt
      961 tgtcgtcgag ggttgtttcc cagagctgct gcggattcat ctcgcccagg cccttgtagc
     1021 gctggagcgt aagtcccttg cggccccact cgaagatcgc gccgagaagt tcgctgggcg
     1081 cgcgcacttc gcggctttcg tccttgcgcg ccacgacgcc gaacttcaga tacgccgcct
     1141 gcagatcggg cgccatgcgg tcaagcttgc gcgcgtcggt cgaggcgatc agcgcgccgt
     1201 cgatggcgac ggcctcgcgc acgccgcgca cgtcgcgcca gaatttgagg ccgccgtcgg
     1261 cagtcggctc gccgtgccag ccgcgctcgg tttcgtcgga cagcagatcg aggcggcgcg
     1321 cgatataagc agccgcttcg gtcgctttgg cggcgtcgcc caggatttcc gggttaaggg
     1381 cgcccgcgat cgccgcctgt tccagcacga agcgcggata gtggcgcggg aagccctcca
     1441 gcgcggtcac cgccacgcgc gccttttcca ccagcgccgc cagatcggcc ccggtcacga
     1501 cctcgccggt gtgcagggtg agggacgcgc ccgccgtacc ttcggcgatc agatgatcct
     1561 gaagttcgtt ctcgtccttc aaatagcgct cggacttgcc gcgcgcggcc ttgtagagcg
     1621 gcggctgggc gatgaagaga tggccgcgtt cgatcagcgc cggcatctgc cggtagaaga
     1681 aggtgagcag cagggtgcgg atatgggcgc cgtccacgtc ggcgtcggtc atgatgatga
     1741 tcttgtgata gcgcagcttg tcgggattga actcctcgct gccgatgccg gtgccgagcg
     1801 cggtgatcag ggtggcgatt tcctggctcg ccagcatctt gtcgaagcgc gcgcgctcga
     1861 cgttgaggat tttcccgcgc aggggcagta cggcctggtt ggcgcggttg cgcgcctgct
     1921 tggcgctgcc gccggcgctt tcgccctcga cgaggaaaag ctcgcatttg gccgggtcgc
     1981 gttcctggca gtcggcgagc ttgccgggaa gggaagcgcc gtcgagcgca cccttgcggc
     2041 gggtgagttc gcgggcgcgg cgcgcggctt cgcgggcggc tgccgcttcg atcaccttgc
     2101 cgacgatggc cttggcctcg gcgggatgtt cctcgaacca gccgctcagt ttttcgagga
     2161 ccacgccttc caccaccggg cggacttcgg acgacaccag cttgtccttg gtctgcgagg
     2221 agaatttcgg gtccggcact ttcaccgaca acacgcaggt gaggccttcg cggcagtcgt
     2281 cgccggcgag cgcggtcttg tccttcttgg cgcccatgga ttcgtcggcg tatttggtca
     2341 ccacccgcgt cagcgccgcg cggaaaccgg ccagatgagt gccgccgtcg cgttgcggga
     2401 tgttgttggt gaagaccagc atgctctcgt ggtagctgtc gttccaggtc agcgcggctt
     2461 ccaccgtgat gccttcgcgt tcggcgatga tgttgatcgg gttcgggatc agcgaattct
     2521 tggcgcggtc gagatagcgc acgaactgtt ccagcccgcc ttcgtaatgc agcttggatt
     2581 ccttcggctc cacgccgcgc ttgtcggcga ggaagatcgt cacgccggaa ttgaggaagg
     2641 ccagctcacg caggcggtgc tccagcgtcg gatagtcgaa ctcggtcatg gtgaaggttt
     2701 ggggcgaggg caggaaggtc acttccgtgc cgcgcttgtt cttcacctcg gctacgacct
     2761 tgagcggcgc ttgcggctcg ccgtggcgga actgcatgta atgctctttg ccgtcgcgcc
     2821 agacgcgcag gtcgagccgt tcgctgagcg cattgaccac cgaaacgccg acgccgtgca
     2881 ggccgccgga gaccttgtag acgctctgct cgaattttcc gccggcatga agctgggtca
     2941 tgatgacctc ggccgccgaa acgccttctt cggagtggat gtcggtcggc acgccgcggc
     3001 cgttgtcgcg caccgtgcag gagccgtcgg gattgaggat cacgtcgatg cggtcggcaa
     3061 agccgccgag ggcctcgtcg atggcgttgt cgacgacttc atagaccatg tgatgcaggc
     3121 ccgagccgtc gtcggtgtcg ccgatataca tgcccggccg cttgcgcacg gcgtcgaggc
     3181 ccttcagcac cttgatgctg tcggcgccgt aatcgtcgga ggcctgttgc gaggcctgct
     3241 gcgcggccag ttccgccgcg tggatttttg ccgcttcgct catctcttca ccttaactca
     3301 ccgccacgcc ggcgccggcg cccgccatga tgactgcggc gcctttgttc aggcgtccgc
     3361 gcgcccttct gctggccaga aacccgcgca cgcgcgaagc aagcgccgcg tatgacaagg
     3421 ttatcgccgg gatcagcacg aacgtcgccg cgcagagctg caaatagccg aaggcgttga
     3481 gacggccgag atcgaccacc gtgggcagca gcgcgacgaa gaacgcaatc gctttgggat
     3541 tgcccagcgt cagcgccatc tgcgccagga aaccctgacg cgcgctcgcg ggaaccaccg
     3601 tttccaggtc ggcgaccggc gcggtccagt atttgtagcc gagatagacc agataggccg
     3661 cgcccatcag cttcacgatc aggaacagtc cgcccatcgc ctgcgcgacc agcgccagcc
     3721 cgaaggccga caaagacatc aggatcaggt cgcccgccgc cgtgccgatc gccgctggga
     3781 tggtggcgcg gaagccgctg cccagggcgc gggccacgat ggcgacgatg cccggtcccg
     3841 gcaccgcgat ggcgatggcg taaagcccgc aatagagaag ataggcgtgc cacgaaagca
     3901 tacggttact cctggcgcac gaagcggccg ttctcgaccg tgaaaacatc ggcgcgcgac
     3961 gaaaggctgt cgaacagcga aagatcggtg cccgtcatcc aggcttgggt gcccagggcg
     4021 aaaagctcct caaataaggc ggcacggcgg cgtgcgtcca gatgtgcggc aatttcgtcg
     4081 agcagcaaca gcggcgcgat cccgccgcgc gcccgcgtca gctcccaggc gtcggccaat
     4141 acgatggaaa ccagcagcgc cttctgctcg ccggtgg
//
LOCUS       DASWPU010000614         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_78577,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000614 DASWPU010000000
VERSION     DASWPU010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78577"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VGC27_13110"
     CDS             complement(<1..343)
                     /locus_tag="VGC27_13110"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY0283550.1"
                     /translation="MSVELNLALVLACGVLALLYGAWTVRWVLAKSPGNARMQEIAAA
                     IQEGARAYLNRQYTTIAIVGVVIFALAWYFLGWEVGVGFLIGAALSGAAGYIGMNVSV
                     RANVRTAEASRQ"
     gene            complement(468..983)
                     /locus_tag="VGC27_13115"
     CDS             complement(468..983)
                     /locus_tag="VGC27_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283551.1"
                     /translation="MTAAGQPAQAAGAKAVAPATSVEMPFLIAPMSKDGKLLGYAYIS
                     SKMITSSPATAIAVREKLVFIQDAYVRDVYAQPIGKAEDPGAVDEALLNARLVAAAKR
                     IVGSGKIVSMVFLSIQFMPLHPGEAPVPPPENASGTGDAAQADRNNGQTDASAAASAP
                     ATSKPAQDAAH"
     gene            complement(1042..2013)
                     /gene="thiL"
                     /locus_tag="VGC27_13120"
     CDS             complement(1042..2013)
                     /gene="thiL"
                     /locus_tag="VGC27_13120"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY0283552.1"
                     /translation="MPNRPSEFALIAQVFAPLAGTPGAFGLTDDAAALPARVGHDLVV
                     TTDALVEGVHFLAGDPPVSIAQKALRVNLSDLAAKGAEPAGYLLALSLPERIGLTWIE
                     EFARGLGEDQKRFSVPLLGGDTTATPGPLTLAITAMGYVPAGAMIRRSGARAGDGVYV
                     SGTIGDAGGGLALLSGESADIGADEAGLLIGRYRLPSPRLALGRALRGVASAALDVSD
                     GLIADLSHIADVSRVRIVVEAGLIPRSSALRALWGDSPEAIVRAATAGDDYEIAFTAS
                     EAKAGEAAGLGGAAVTRIGRVEEGAGVALLDSDGREIEVAHTGFTHF"
     gene            complement(2018..2512)
                     /gene="nusB"
                     /locus_tag="VGC27_13125"
     CDS             complement(2018..2512)
                     /gene="nusB"
                     /locus_tag="VGC27_13125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY0283553.1"
                     /translation="MSAKPDASARRAARLAAIQALYQMELAGVDAETVAEEFAAHRFG
                     REPEMLALGEPDEAFFSDIVRGVPHRQAEIDAAITKCLASDWRLSRVDSILRAILRAG
                     AYELIGRADVPVKVIIDEYVDLSYAFFSGEEPAFVNAALDKLARRKRAAEFGEAPPED
                     EPGF"
BASE COUNT          470 a          885 c          834 g          415 t
ORIGIN      
        1 cctggcgcga cgcttcggcg gtacgcacat tggcgcgcac cgagacgttc atgccgatat
       61 agccggccgc tcccgacaaa gccgccccga tcaggaaccc gacgccgact tcccagccga
      121 ggaagtacca ggcgagcgcg aagatgacga cgccgacgat cgcaatcgtc gtgtattggc
      181 gattcagata ggcgcgcgcg ccttcctgaa ttgccgcggc gatttcctgc atgcgggcgt
      241 tgccggggct cttcgccaga acccagcgga ccgtccatgc gccatacaac agcgccaaaa
      301 cgccgcaagc caataccaat gcaagattaa gctccacgct catggtgttc ctcgtcccga
      361 tccttggacc aaattaaacg gcgcacaccg ccgaattcca cagcgtgcgg gaatcccgcc
      421 gcttcgaagg cggccggaac atgccaaatg cgggtggggg aagcaactta atgggctgcg
      481 tcttgcgccg gtttagatgt cgcgggggct gacgcagcgg cggaggcgtc ggtttggccg
      541 ttgttccggt cagcctgtgc ggcatcgcct gttccggagg cattttccgg cggcggaacc
      601 ggggcctcac cgggatgcag cggcataaat tggatggaga ggaaaaccat gctcacgatc
      661 ttgccgctcc ctacgattcg cttggccgcg gcgaccaggc gagcattcag aagcgcctcg
      721 tcgacggccc cggggtcttc cgccttgccg atgggctgcg cgtagacgtc gcgcacatag
      781 gcgtcttgga tgaagacgag cttctcgcgc acggcgatgg cggtggctgg cgacgacgtg
      841 atcatcttgc tcgaaatata agcatatcca agcagtttcc cgtccttgct catgggagcg
      901 ataaggaacg gcatctcgac actggtggcg ggcgcgactg ctttcgcgcc ggccgcctga
      961 gcgggctggc ccgctgccgt catgacaagg agcaaaagga aaacgagaac cccgcgcatg
     1021 ccggagcctc gcaaatcgcg gctaaaaatg tgtgaatccg gtatgagcga cctcgatttc
     1081 gcgtccgtcc gaatcaagca gggcaacgcc cgcgccctct tccacccggc cgatgcgggt
     1141 cacggcggcc ccgccaagcc ctgccgcttc gccggccttt gcctcggagg cagtgaatgc
     1201 gatttcgtaa tcgtcgcctg ccgtggcggc tcggacgatg gcctccggcg aatcacccca
     1261 cagtgcccgc agagcggacg agcgggggat tagccctgcc tcgacaacga tgcgcacccg
     1321 cgatacgtcg gcgatatgtg acagatcggc gatgagacca tcagatacat ccagcgcagc
     1381 gctggcgacg ccccgtaaag cccggcccag agccaggcgc gggctgggca ggcgataacg
     1441 cccgatcagc agcccggctt catctgcgcc gatgtccgcg ctctcgccac ttagaagcgc
     1501 cagcccgccg ccggcatcgc cgatagtgcc gctgacatac acgccgtccc ctgccctggc
     1561 gccggagcgg cggatcatgg cgcccgccgg aacatatccc atcgccgtga tcgccagcgt
     1621 cagcggccct ggcgtcgcgg tcgtgtcgcc gccgagcaac ggcacggaaa acctcttctg
     1681 gtcctcgccc agcccgcgcg caaactcctc gatccaggtc aagccgatgc gctccggcag
     1741 cgacagcgcc agcagataac cggcaggctc cgcccctttt gccgcgagat cggacaggtt
     1801 tacgcgcaac gccttctggg cgatcgagac gggcggatcg ccggcaagaa aatgcacgcc
     1861 ttcgaccaga gcgtccgtcg tcacgaccag atcgtggccc acgcgcgccg gcagcgcagc
     1921 ggcatcgtcc gtcagcccga atgcgcccgg cgttcccgcc agcggtgcaa agacttgcgc
     1981 gatcagcgcg aattcggagg gtcggttggg cattgggcta aaatcccggc tcgtcctccg
     2041 gcggggcttc gccgaattcc gcggcgcgct tgcgccgtgc cagcttgtcc agcgccgcgt
     2101 tcacgaaagc aggctcctcg ccggagaaga aagcgtagga cagatctaca tattcgtcga
     2161 tgatcacctt caccggcacg tcagcccggc cgatcagttc ataggcgccc gcccgcagga
     2221 tggcgcgcag gatcgaatcc acccgcgaaa gccgccagtc gctggcaagg catttggtga
     2281 tggcggcgtc gatttccgcc tgacggtgcg gcacgccgcg cacgatgtcg gaaaagaaag
     2341 cttcgtccgg ctcgcccagc gccagcatct ccggttcgcg gccgaagcga tgggcggcga
     2401 attcctccgc caccgtttcg gcgtccacgc cggccagttc catctgatag agcgcctgga
     2461 tggcggccag ccgcgccgcg cgccgcgccg acgcgtcggg cttggcgctc atcggcggac
     2521 gccgaggcga tggcgcagtt cgatcagtga caggcaggcg cgggcggcgt cgccgccctt
     2581 gtcgcgcagc gtacggtcgg cgcg
//
LOCUS       DASWPU010000615         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_10887,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000615 DASWPU010000000
VERSION     DASWPU010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10887"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VGC27_13130"
     CDS             complement(<1..1128)
                     /locus_tag="VGC27_13130"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0283554.1"
                     /translation="MRLRLSWFLLLLACLTAPAWAKDTITVGLQLEPPILDPTASADS
                     AIGEVVYANVFEALTGANRDGSVRPWLASSWTISPGGLAYVFHLRPGVRFHNGGVLSA
                     QDVKFSLDRARAANSTNPQKPRLQAIRSVEIVDPLTVRIWLHEPDSGLLFTLSLPSFV
                     IVAPDTAGGNKQTPIGTGPFRFDSWKRGEAIALVRFPAYWGKPARLEHAVFRFMADST
                     AAYAAVKSGDVDVFPDFPAPESIAQIKRDPRLAVSIGSTEGQVILAINNRKPPFDNLL
                     VRRAVSHAIDRKAVIDGAFYGLGTPIGSHYAPQDEGYVDLTARYPHDPARARALLAQA
                     GFPNGFTATLKLPPPMYARRSGEIIASQLAAVGIKVKLENLE"
     gene            complement(1178..>1954)
                     /locus_tag="VGC27_13135"
     CDS             complement(1178..>1954)
                     /locus_tag="VGC27_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0283555.1"
                     /translation="GPLLVLVHGFGDALGTWDGWTAALKDQYRIIRVDLPGHGLTQAP
                     ATYRIDVEAYADLIDELAAKLSLPKFAIAGNSLGGAVAWEFAVRHPSRLNALILVDAA
                     GWPSKGLTKNPPLAFRILQYKLGRDILKTIDNKPLIRDSLRREVGDPSVITPQLVERW
                     AELQRAPGHREILMSGPVAGKGLAAKEKLAGIKLPALVLWGENDPLLDLDGGKKFAAA
                     IPGARFVVYPHVGHLPQIESPIPSARDVALFLGSPAAGTN"
BASE COUNT          376 a          628 c          625 g          325 t
ORIGIN      
        1 ttcgagattc tcgagtttga ctttgatccc cacggcggcc aattgagagg cgatgatctc
       61 accgctgcgc cgggcgtaca tgggcggcgg cagcttgagc gtggccgtga acccgttcgg
      121 aaagccggcc tgcgccagca acgccctcgc ccgcgcggga tcgtgcggat agcgcgccgt
      181 gagatcgaca tagccttcgt cctgaggcgc ataatggctg ccgatcggcg tccccaggcc
      241 gtagaacgcg ccgtcgatca ccgccttgcg gtctatggcg tgcgaaaccg cccggcgcac
      301 gagaagattg tcgaacggcg gcttgcggtt gttgatggcg aggatcacct gcccctccgt
      361 cgacccgata gagacggcaa ggcggggatc gcgcttgatc tgggcgatgc tctccggcgc
      421 gggaaaatcc ggaaacacgt ccacgtcgcc cgatttcacc gcggcgtagg ccgccgtcga
      481 atccgccatg aagcggaaga cggcatgttc caggcgcgca ggtttgcccc agtaggcggg
      541 aaaccggaca agcgcaatcg cttcgccgcg cttccaactg tcgaaccgga acggccccgt
      601 tccaatcggg gtctgcttgt ttccgcctgc cgtgtccggc gcgacgatga cgaaggatgg
      661 caaggacaac gtgaacaaga ggccgctgtc gggttcgtgc agccatatgc ggaccgtcaa
      721 gggatcgaca atctcgaccg agcggattgc ctgcaggcgc ggcttttgcg ggttcgtcga
      781 gttcgcggcg cgcgcgcggt ccaggctgaa tttgacatcc tgcgcagaca gcacgccccc
      841 gttatgaaac cggacaccgg ggcgaagatg gaagacatat gccagcccgc cgggcgagat
      901 cgtccaggac gacgccagcc acggacgcac cgagccgtcg cgattggcgc cggtcagcgc
      961 ctcgaacacg tttgcgtata cgacttcgcc gatcgccgaa tccgcgctgg ccgtgggatc
     1021 gaggatcggc ggctcaagtt gcagtccgac cgtgatggtg tccttggccc aagccggagc
     1081 cgtcaggcaa gccagcagga ggagaaacca agaaagcctg agcctcaagc aaacacccgg
     1141 gaaagcagca caaaagttat tcggccgacg gctatgctca gttcgttccc gccgccggac
     1201 tgccgagaaa cagagcgacg tcacgggccg agggaatggg actctcgatc tgcggcaaat
     1261 ggccgacatg gggatatacc acaaaccggg cgcccggaat cgccgcggcg aacttcttgc
     1321 cgccgtcgag atcaagcaac ggatcgttct cgccccacaa gaccagcgcg ggcagcttga
     1381 tcccggccag tttttccttg gctgccagcc cctttcccgc caccggcccc gacatcaata
     1441 tctcgcggtg acccggcgcg cgctgcagtt ccgcccagcg ctccaccagc tgcggcgtga
     1501 tgacggaagg atcgccgact tcgcgcctca gcgagtcccg gattagaggc ttgttgtcga
     1561 ttgtcttcag gatatcccgg ccgagtttgt attgcaggat gcggaatgcc agcggcgggt
     1621 tcttcgtcag ccccttgctc ggccagccgg cggcatcgac caagatgagc gcattcaggc
     1681 gcgaagggtg acgaaccgcg aactcccaag ccaccgcgcc gccgagcgag tttcccgcaa
     1741 tggcgaattt cggcagggat agcttggccg ccagttcgtc gatcagatcg gcgtaagcct
     1801 ccacgtcaat ccggtacgtc gcgggcgctt gcgtcaggcc gtgacccggg agatcaacgc
     1861 ggatgatccg gtactgatct ttcagggctg cggtccagcc gtcccacgtt ccaagcgcat
     1921 ccccgaaacc gtgaaccagg acgagaagcg ggcc
//
LOCUS       DASWPU010000616         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_85313,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000616 DASWPU010000000
VERSION     DASWPU010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85313"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..549)
                     /locus_tag="VGC27_13140"
     CDS             complement(4..549)
                     /locus_tag="VGC27_13140"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0283556.1"
                     /translation="MTVASPVQACAEFNALAETKGWPTRCHCGETFERSDLNALCELW
                     RSKAANGVPPRSAFGMRVLAPYLRNITILEREGENGTKRYRFRLFGSTLALLFGEHTG
                     RTLDQMVSADMLPSWLAFYDTVLACRQPLRVDTHYRTASEGYVRGEILAAPLADDSGE
                     VRMVLAATYVGFQDGVLSPFG"
     gene            complement(648..>1721)
                     /locus_tag="VGC27_13145"
     CDS             complement(648..>1721)
                     /locus_tag="VGC27_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0283557.1"
                     /translation="ALPLYHATGGVCAVGMALTVGGAVIIRRKFSAREFWDDCRSYRA
                     TLFPYVGELCRYLLNVPPAPNDRRHMLRAITGNGLRPEIWPAFQERFAIPRIVEFYGA
                     TEGNVAMLNYDGKVGAVGRVPRYMRWYMQIRLVRFDIAEEAPVRDPGGFCIECDPGEV
                     GEAAGKITTRPGHGFEGYTRSADTGKKILHDAFEKGDAWFRTGDLLRRDEQGYFYFVD
                     RIGDTFRWKGENVATGEVAKALGSVAGIKEVNVYGVVVPGADGRAGMAALVAAPGFDP
                     ASLGEKIAADLAPFARPMFLRLRPEMEITGTFKLKKADLVKDGFDPGRIDDPLYWYDP
                     ASGRYEPVTQEVYAGIAAGQVKL"
BASE COUNT          316 a          569 c          543 g          293 t
ORIGIN      
        1 actctacccg aaaggcgaga gcacgccgtc ctgaaagcca acataggttg cggccaggac
       61 catccgcacc tcgccggaat cgtcggccaa aggcgccgcg aggatttcac ccctgacata
      121 gccctcggac gccgtgcggt aatgggtgtc cacgcgcaac ggctggcggc acgccagaac
      181 cgtgtcgtag aaggccagcc agctcggcag catgtcggcg gacaccatct ggtcgagcgt
      241 gcggcccgta tgttctccga acagaagcgc caaggtggaa ccgaacaagc ggaagcgata
      301 acgctttgtg ccgttctctc cctcgcgctc caggatggtg atgttgcgca agtatggcgc
      361 cagcacgcgc atgccgaagg cggagcgcgg cggcacgccg ttcgccgcct tcgaccgcca
      421 cagttcgcaa agcgcgttga gatcgctccg ctcgaaggtc tcgccgcaat ggcagcgggt
      481 cggccagccc ttcgtttcgg cgagagcgtt gaactccgca caagcttgga cggggctggc
      541 tacagtcatg gcgggattct gcattgcagg gtttacgaat ccgtaagtaa atctcgttaa
      601 cgcggcaggg atgcctagtt aactccatta aaaccgccat ccgagtctca gagctttact
      661 tggcccgcgg cgatccctgc gtaaacttcc tgggtcacgg gctcgtagcg tccgctcgcc
      721 ggatcgtacc agtagagcgg atcgtcgatg cggccgggat cgaatccgtc cttcaccaga
      781 tcggctttct tgagcttgaa agtgccggtg atctccattt ccggccgcag cctgagaaac
      841 atcggccgcg cgaacggcgc cagatcggcc gcgatcttct cgcccagaga ggccgggtcg
      901 aagcccggcg ccgccaccag cgccgccatt cccgcgcgcc cgtccgcgcc gggaacgacg
      961 acgccgtaaa cgttgacttc cttgatgccg gcaacgctcc cgagcgcctt ggcgacctcg
     1021 ccggtggcca cgttctcacc cttccagcgg aaggtatcgc cgatgcggtc cacgaaatag
     1081 aaatagcctt gttcgtcgcg gcgcagcaga tcgccggtgc ggaaccaggc gtcgcccttc
     1141 tcgaacgcgt cgtgcaggat cttcttgccg gtgtcggcgg aacgcgtata gccctcgaag
     1201 ccgtgaccgg gacgggtggt gatcttgccg gccgcttcgc ccacttcgcc cgggtcgcat
     1261 tcgatgcaga agccgccggg gtcgcgcacc ggtgcttcct ccgcgatgtc gaagcgcacc
     1321 agcctgatct gcatgtacca gcgcatgtag cgcggcaccc ggccgaccgc gcccaccttg
     1381 ccgtcgtaat tcagcatcgc gacattgcct tcggtggcgc cgtagaactc gacgatccgc
     1441 ggaatggcga agcgctcctg gaacgcgggc cagatttccg ggcgcaagcc gttgccggtg
     1501 atcgcgcgca gcatgtggcg cctgtcgttc ggagcgggcg gcacattcag cagataacgg
     1561 caaagctcgc cgacataagg gaagagcgtg gcgcgatagc tccggcaatc gtcccagaat
     1621 tcgcgcgccg agaatttgcg ccggatgatc accgcgccgc cgacggtgag cgccatgccc
     1681 acggcgcaga ccccgccggt ggcgtgataa agcggcaagg c
//
LOCUS       DASWPU010000617        12574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_29500,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000617 DASWPU010000000
VERSION     DASWPU010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 12574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12574
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29500"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VGC27_13150"
     CDS             complement(<1..1078)
                     /locus_tag="VGC27_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0283558.1"
                     /translation="MSYDVIIIGGGHAGCEAAAASARAGAKTLLLTHKRATLGEMSCN
                     PAIGGVGKGHLVREIDAADGLMGRVADAAGIQFRMLNRRKGPAVRGPRAQADRKLYRQ
                     AMRAALEATANLEIRESAAEDLIIADGRVAGVITAEGEALRAGAVVLTTGTFLKGLIH
                     CGELKVAAGRAVAGHSKFEGDGIEAPSIGLANTLYGLGFALGRLKTGTPPRLDGKTID
                     WASLEVQHGDEPPSPFSFLTKAITTPQIACHITRTNEQTHAVIRANLDRAPLYSGQIE
                     GRGPRYCPSIEDKVVRFAHRASHQIFLEPEGLDDDTVYPNGISTSLPEDVQHALLKTI
                     PGLENVAVLRPGYAIEYDFVDPREL"
     gene            complement(1143..2459)
                     /gene="mnmE"
                     /locus_tag="VGC27_13155"
     CDS             complement(1143..2459)
                     /gene="mnmE"
                     /locus_tag="VGC27_13155"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY0283559.1"
                     /translation="MSRDDTIFALASAAGRAGVAVFRVSGPVAGDCVKHLTGHDIPPS
                     HRAVLRSLRGRRGAIDEALVLWFEAPASLTGEDVAEFQVHGGRAIAEALAAEIGTLPG
                     CRPAEPGEFTRRAVEHGKLDLTQAEAIADLVAAETEGQRRQALRQYDGALGALYEGWR
                     RELIKALAWAEAVIDFADEELPDDVLAETRRTAERVREEVETHLNDARRGELVREGLF
                     LTVIGPPNAGKSSLVNALAGRDVAIVSETAGTTRDVIEVRMDLGGYAVILADTAGLRA
                     ASEAVEAEGVRRALARAEAADLVLLLIDGSEEDTAVVLPGNMVNKPSLTIWNKADLPW
                     PVPREGLRLSLKTGEGLDVVIAAIAAAAKERLDAPGEAPLLTRPRHRHALGEAAAALQ
                     RAAGAREPELFAEDLRLALRSLGRITGRVDVEELLDTIFKDFCIGK"
     gene            2751..4805
                     /locus_tag="VGC27_13160"
     CDS             2751..4805
                     /locus_tag="VGC27_13160"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0283560.1"
                     /translation="MSTNRLAEETSPYLLLHKDNPVEWRPWGPEAFQEAEISGKPILL
                     SIGYTACHWCHVMNKESFADPETAAAMNQGFINIKVDREERPDIDQLYQTAAQTMGYQ
                     GGWPLTIFLTPKGEPFFVGGYFPNEERFGQPPFKKVLEDVSGRFRDQAEAVATDATRI
                     SEAVGAQWTRDLRGPFDPRLLDVVAVHIAQRFDIFYGGVTGAPKFPQTALTEAIWRSF
                     LRTGAMQFPQLVQTTLDTMCLASIYDHIGGGFHRYAVDERWLIPHFEKMLYDNALMID
                     LLTTVGVHNRVPLYRVRVEETIGWVLREMMVGDAFASSIDADVNGEEGAYYLWGEAEI
                     DATLMGTFAQRFKDVYAIRNEGPFNGRNIISRTGPFPFPLPDADEALLKRQRELLLAA
                     RNQTRTAPLRDDKVLADWNGMMIAALVNAGTTQGNPPWIAAAERAFAFIVKAMAEGDR
                     LYHSWRDGKRGHMGFADDYAQMARAALSLWEATGNKQYLDHAKRWTLVLNEHFWDGKN
                     GGYCQSADDDAPLLHRVRTVFDQATPCANGTMVGVLAKLHMATGETSHRDRAGNIVTA
                     FAGELGRSVLAMGTFLNGLETLLTGLQIVIVGPKDSPKTQDLVAAVLGRSLPNRLLLV
                     VGPAESLPETHPAFGKKMENGQPTAYVCQHQNCSAPIANAPTLSQLLQLPQRPPQGTR
                     PQ"
     gene            4877..5575
                     /locus_tag="VGC27_13165"
     CDS             4877..5575
                     /locus_tag="VGC27_13165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0283561.1"
                     /translation="MSGQQVTIGGPDGSFAAYLASSAAGRGPGIVVIQEIFGVNAVMR
                     AVADEFAASGFFALVPDLFWRLEPGVELTDRTDAEWQRAFALMNRFDAALGVTDIQAT
                     IDHLRTVSGCTGKVGAVGYCLGGLLAYLTAARTDSDATVGYYGVNIQAFLGEATAIEK
                     PLMLHLAGKDEFMPPPAQKEIAEGLAAHPLVTLHHYPEMNHAFARPGGKHYDQANAGL
                     ANSRTATFFRRYLS"
     gene            5736..6707
                     /locus_tag="VGC27_13170"
     CDS             5736..6707
                     /locus_tag="VGC27_13170"
                     /inference="COORDINATES: protein motif:HMM:NF008024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY0283562.1"
                     /translation="MKAIRFAKAGGPEVLTLHDVELPPPAPGQVRVRHTAIGINFLDT
                     YHRSGLYPVALPSGLGMEAAGTVEALGEGVAELKLGDRVGYCSGPIGAYAEANNVPAD
                     KVVKLPATVSDETAAASLLKGMTAQYLLKRTYAVKPGQTILIHSAAGGVGLIAGQWAK
                     HLGATVLGTVGSDDKIDLAKAHGYDQVLNLRMDDWVARVRELTNGEGVPVVYDSVGKD
                     TFAGSLDCLAVRGMLVSFGNSSGPVPAFAPAILSAKGSLYLSRPTLFHYTRTPRELQE
                     TAADLFAMIASGAVKIEINQRFALKDARAAHEALHARKTTGATILIP"
     gene            complement(6834..8090)
                     /gene="rho"
                     /locus_tag="VGC27_13175"
                     /pseudo
     CDS             complement(6834..8090)
                     /gene="rho"
                     /locus_tag="VGC27_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020054903.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
     gene            complement(8359..8802)
                     /locus_tag="VGC27_13180"
     CDS             complement(8359..8802)
                     /locus_tag="VGC27_13180"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY0283563.1"
                     /translation="MKEFLLDHYLVIKALHVIAVIAWMSGMFYLPRLFVYHTQTKPGA
                     ADYERFMVMERKLLKIIMAPAVITVWLLGLTMAWLMDYWSLHWFEMKLALVAAMTLTH
                     FSYAIWAKDFALGRNQRSARFFKVWNEVPTVLMIGIVVLAIVKPI"
     gene            complement(8806..9819)
                     /gene="hemH"
                     /locus_tag="VGC27_13185"
     CDS             complement(8806..9819)
                     /gene="hemH"
                     /locus_tag="VGC27_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY0283564.1"
                     /translation="MKLAVVLFNLGGPDSPEAVEPFLRNLFGDPAILSLPSFVRKPLA
                     RFIAKRRAPIARAIYAKIGGRSPILEETQAQARALEKALDEHEARAFIAMRCWKPFSD
                     EAARAIKAWGAEHIVLLPLYPQFSTTTTGSALTDWRRAASAAGLTAPQSGPCCYPRLP
                     GFIEAMAGLTLEAMAKTQPGLSYRLLLSAHGLPERIVAKGDPYRWQVEQTAAALVERL
                     GVADWTVCYQSRVGPVKWITPATDAEIRRAGADGKGAIVVPISFVSEHSETLVELDME
                     YGDLARQAGVRDYLRVPTVSTRPEFISGLARLVRQSLAHPRESAGGRLCPAAQGRCGH
                     GRN"
     gene            complement(9816..10850)
                     /gene="hemE"
                     /locus_tag="VGC27_13190"
     CDS             complement(9816..10850)
                     /gene="hemE"
                     /locus_tag="VGC27_13190"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY0283565.1"
                     /translation="MQSSKKLLETLNGKVLTPPPIWLMRQAGRYLPEYREARNKAGSF
                     WKLCLTPELAAEVTLQPVRRFGFDAAIIFSDILVVPAALGVSVTFEDGAGPRLSPVAS
                     IAGQMDDPEQWGAMLEPVYEALRITRTGLGDQTALLGFAGAPWTLASYLAAGAGGDEQ
                     KAAKLWAYRDPVGFAKLIDVLAECVSQHLIGQLKAGADAVQLFDSWASGLPPQAFARW
                     IVEPTKKIVAKVRAAVPQARIIGFPRAATLKGYEHYTAETGVDAVSLDTAVPVGWAAR
                     TLAPQVVLQGNLDPVALVAGGSALAEAVEHILKAAGEVPFIFNLGHGILPETPTQHVE
                     EMIRLVRSAR"
     gene            11105..11926
                     /locus_tag="VGC27_13195"
     CDS             11105..11926
                     /locus_tag="VGC27_13195"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY0283566.1"
                     /translation="MNRVFHLHLVSDATGETLHAVTKAALAQFEGLTVQEHSYTLVRS
                     TRQLLRAIEYIRDHPGLVLFTMVHQELRLELVNQCTLMNVPYFDLMDGPVSLMRRFFG
                     ADQTYRPGGQHEIDQHYLQRIEALNYTIQHDDGQVLDDLAQAEVILAGASRTSKTPTC
                     VYLAMRGIRAANVPLVLGLPAPAQLLACTGPLIVGLWASPERLVQIRRNRLATIGEQK
                     LTDYVDLEKVRSEVMKTRRLYEEYSWPTIDVTRRSVEETAATVLNLLAERRGENP"
     gene            11923..12516
                     /locus_tag="VGC27_13200"
     CDS             11923..12516
                     /locus_tag="VGC27_13200"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY0283567.1"
                     /translation="MNALILASASAVRARLLREAGVPFDVRPAEIDEGDIKALLAGKL
                     GADGIAGVLAERKAIKISTAHPQALVLGCDQTLAFDGRLIDKAADLAAAQALLHEMRG
                     KEHSLFTACVLAQDGVPVWRRLEHSRLWMRPFSDAFLDDYLRTEGNKLLGSVGCYHLE
                     GSGIQLFERVEGDYFSVLGLPLIPLLAALREHRMIGR"
BASE COUNT         2292 a         4129 c         3823 g         2330 t
ORIGIN      
        1 tgagctcgcg cggatcgacg aaatcatatt cgatggcata gcccggccgc agcactgcaa
       61 cattttccag ccccggaatg gttttcagca gcgcatgttg tacgtcttcg ggcaaagagg
      121 tggaaatccc gttgggatag accgtgtcgt cgtccagccc ttccggttcc aggaaaatct
      181 gatggctggc gcgatgggcg aagcgcacca ccttatcctc gatggaggga caatagcgcg
      241 ggccgcgccc ttcgatctgg ccggaataga gcggcgcgcg gtccagattg gcgcggatca
      301 ccgcgtgggt ctgctcattg gtccgggtga tgtggcaggc gatctgaggg gtggtgatcg
      361 ccttggtcag gaacgagaaa ggcgagggcg gctcgtcgcc atgctgcact tccaggcttg
      421 cccaatcgat ggtcttgccg tcgagcctgg gcggggttcc ggtcttcagc cggcccaggg
      481 caaagccaag cccatacagc gtgttagcaa gcccaatcga cggcgcttcg atgccgtcgc
      541 cttcgaactt ggagtgcccg gccacggcgc gcccggccgc gaccttcagc tcgccgcaat
      601 ggatcaagcc cttcaggaat gtcccggtgg tcagcaccac ggctccggca cgcaaagcct
      661 cgccctcggc cgtgataact cccgccactc gcccgtctgc aataatcaga tcctcggcgg
      721 cgctttcgcg aatttccagg tttgccgtgg cttcaagcgc ggcccgcatc gcttggcgat
      781 aaagcttgcg gtcggcctgg gcgcgcggcc cgcgcacggc cgggcctttg cgccggttga
      841 gcatgcggaa ctggattccg gcggcatccg cgacgcggcc catcagcccg tcggcggcat
      901 cgatctcgcg caccagatgg cctttgccga ccccgccgat ggcgggattg cacgacatct
      961 cgcccagcgt ggcgcgcttg tgggtgagga gcagggtctt cgccccggcg cgcgccgatg
     1021 ccgccgccgc ctcgcagccg gcatggcctc cgccgatgat gatgacgtcg tagctcatgg
     1081 cgcgctcaat aagtaaggga ccttctttga gcaagcatgg ggcgctgttt cacgtgaaac
     1141 aattatttcc cgatgcagaa atccttgaag atcgtatcca gcagttcttc cacatcgacc
     1201 cggccggtga tgcggccgag cgagcgcagc gccaggcgca aatcctcggc gaagagttcc
     1261 ggctcgcgcg cgccggccgc ccgttgcaag gccgccgccg cttcgcccaa ggcatgacga
     1321 tggcgcggcc gggtcagcag cggcgcctcg cccggcgcgt ccagccgctc tttggccgcg
     1381 gcggcgatgg cggcaatcac gacgtccaaa ccctctccgg tcttgagcga cagtctcagc
     1441 ccctcgcgcg gcaccggcca gggcaggtcg gccttgttcc agatcgtcag agagggttta
     1501 ttaaccatat ttcccggtag aacgactgcc gtatcctctt cgctgccgtc tatcaacagc
     1561 agcaccaggt ccgccgcttc ggcccgcgcc aaggcccggc gcacgccttc ggcttctacc
     1621 gcctcgctgg ccgcgcgcag cccggcggta tcggccagga tcacggcata accgcccaga
     1681 tccatccgca cctcgatcac gtcccgggtg gtgccggcgg tttcggagac aatcgccacg
     1741 tcgcgcccgg caagggcatt caccagcgaa ctctttccgg cgttcggtgg gccgatcact
     1801 gttaggaaca atccctcccg caccaattcg ccgcgtcgcg catcgttcag atgggtttcc
     1861 acctcttcgc ggacacgttc cgccgtccgg cgcgtctcgg ccagaacgtc gtcgggcagt
     1921 tcctcgtccg caaaatcgat gaccgcttcc gcccaggcca gcgctttgat cagctccctc
     1981 cgccagccct catagagcgc gcccagcgct ccgtcatatt gccgcagggc ttggcgccgt
     2041 tgcccctcgg tctcggccgc aaccagatcg gcaatggcct ccgcctgggt tagatcgagc
     2101 ttgccgtgct cgaccgcgcg tcgggtgaac tcgcccggct cggctggacg gcagccgggc
     2161 agggtgccta tctccgccgc cagagcttca gcaatggcgc gtccgccatg aacctggaac
     2221 tcggcgacat cttcgccggt caggcttgcc ggagcttcaa accacagtac cagagcctcg
     2281 tcgatcgctc cccggcggcc gcgcaaggac cgcaatacgg cccgatgcga tggaggaata
     2341 tcgtgtcctg tcaggtgctt aacacagtcc ccagcgaccg gcccggaaac ccggaacacc
     2401 gccaccccgg cgcgtccggc cgcgctcgcc agggcaaaga tggtgtcgtc ccggctcacc
     2461 ggcgcctgca ccaatcgccg gcacccacac tgcccggcgc cacgcgtccg ctcagcggga
     2521 ttggcgatct gttcgttccc ggcgcccctg gcatgcgctg tgcccttccg gccatgactt
     2581 gctcccgctc tccaccggcc tccactcgat ttcgcccccc gtcttaatgg tccgccgcct
     2641 tccagcttgt gagtgcgcgc gtcctcgcgc taaaggaagc ttggtctagt tcagtccatc
     2701 aacgcaatgc gtgcgtcccg ccgcttgcgc gccaattgga aaaaaaacag atgagcacca
     2761 atcgcctcgc agaggaaacc agcccgtatc ttctccttca caaggataac ccggtcgaat
     2821 ggcgcccttg ggggcccgaa gccttccagg aagcggaaat cagcggcaag ccgatcctct
     2881 tgtccatcgg ttacaccgcc tgccattggt gccatgtgat gaacaaggaa agtttcgccg
     2941 atcccgagac cgccgcggcg atgaaccaag gcttcatcaa catcaaggtc gaccgcgaag
     3001 agcgtcccga catcgatcag ctttatcaga ccgccgctca gaccatgggc tatcagggcg
     3061 gctggccgct caccattttc ctgaccccga agggcgaacc gtttttcgtc ggcggctact
     3121 tccccaacga ggaacgcttc ggccagcctc cgttcaagaa ggtgctcgaa gacgtctccg
     3181 gccggttccg cgaccaggcc gaggccgtcg ccaccgacgc gaccaggatt tccgaagcgg
     3241 tcggcgccca atggacgcgc gacctcagag gcccgttcga tcccaggctg ctggatgtcg
     3301 tcgccgtgca tatcgcgcag cgtttcgaca tcttctacgg cggggtcacc ggcgcgccga
     3361 aattccccca gaccgcgttg accgaagcca tctggcgcag cttcctgcgc accggcgcga
     3421 tgcagttccc gcagctggtg cagaccacgc tcgacaccat gtgcctcgcc tcgatctacg
     3481 atcacatcgg cggcggcttt caccgctacg cggtcgacga gcgctggctg attccgcatt
     3541 tcgaaaagat gctctacgac aacgcgctga tgatcgatct gttgacgacg gtcggcgtcc
     3601 acaaccgcgt cccgctctat cgcgtgcgcg tcgaggagac gatcggctgg gtgctgcgcg
     3661 agatgatggt cggcgacgcc ttcgcttcca gcatcgatgc cgacgtgaac ggcgaagaag
     3721 gcgcctatta cctgtggggc gaggcggaaa tcgacgccac gctgatgggc accttcgccc
     3781 agcgcttcaa ggacgtctac gccatccgca acgaaggccc gttcaacggc cgcaacatca
     3841 tcagccgcac cggcccgttc cccttcccgc tgcccgacgc cgacgaagcc ctgctcaagc
     3901 gccagcgcga acttttgctc gccgcccgca atcagacccg cacggcgccg ctgcgcgacg
     3961 acaaggtgct cgccgactgg aacggcatga tgatcgccgc cctggtcaac gccggcacca
     4021 cccagggcaa tccgccatgg atcgccgcgg ccgagcgcgc cttcgccttc atcgtcaagg
     4081 cgatggcgga gggcgaccgc ctttatcatt cctggcgcga cggcaagcgc ggccatatgg
     4141 gcttcgccga cgactatgcg cagatggcgc gcgcggcgct ctccttgtgg gaggccacgg
     4201 gcaacaagca gtacctcgat cacgccaagc gctggacctt ggtgctgaac gaacatttct
     4261 gggacgggaa gaacggcggc tactgccagt cggcggacga cgatgcgccg ctattgcacc
     4321 gtgtacggac ggtcttcgat caggccacgc cctgcgccaa cggaaccatg gtcggcgtgc
     4381 tcgccaagct tcatatggct accggcgaaa cgagccaccg cgaccgcgcc ggcaatatcg
     4441 tcaccgcctt tgccggcgaa ctcggccgct cggtgctggc gatgggaacc ttcctcaacg
     4501 gactggaaac gctgctcacc gggctgcaga tcgtgatcgt cggtcccaag gacagcccca
     4561 agacgcagga tctcgtcgcg gcggtcttgg gccgcagcct gcccaaccgc ttgctgctgg
     4621 tggtcggtcc cgcggagtcc ttgcccgaaa cccatcccgc cttcggcaag aagatggaga
     4681 acggccagcc caccgcttat gtctgtcagc atcagaactg ctcggcgccg atcgccaacg
     4741 cgccgacgct cagtcagttg ctgcagttgc cgcagcgtcc gccgcaggga acccgcccgc
     4801 aatagcggat cgcaggctgg cctcaacgcg ccgctccgtc taacctcaca gcgaggttgg
     4861 acggagcgtt gctgtgatgt cgggacaaca ggttacgatc ggcggaccgg acggttcctt
     4921 tgccgcctat ctggccagct ccgccgccgg ccggggcccc ggcatcgtcg tcatccagga
     4981 gattttcggc gtcaacgcgg tgatgcgcgc cgtcgccgat gaattcgccg caagcggctt
     5041 cttcgccctg gtccccgatt tattttggcg tctcgaaccc ggcgtcgaac tcactgatcg
     5101 gaccgacgcg gaatggcagc gtgccttcgc tctgatgaac cgcttcgatg cggctcttgg
     5161 cgtgacggac atccaggcga cgattgatca tctgcgcacg gtctccggtt gcacgggcaa
     5221 ggtgggcgcg gtcggctatt gcctgggcgg cttgctcgcc tatctcaccg ccgcgcgcac
     5281 cgacagcgac gccacggtcg gttactacgg cgtgaacatc caggctttcc tgggtgaagc
     5341 tacggcgatc gaaaagccgc tgatgctgca cctcgcgggc aaggacgaat tcatgccgcc
     5401 gccagcgcag aaagagatcg cggaagggct ggccgcccat ccgctcgtca cgctccacca
     5461 ttatccggaa atgaatcacg ccttcgcccg gcccggcggc aagcattacg accaggccaa
     5521 tgccggtctc gccaacagcc gcaccgccac cttcttccgc cggtatttga gctgacctgc
     5581 gattctaagc ggcattgatc ggcgcgcccg agcgctcatc tttcctgtag ccggatcaag
     5641 ctatcgccgg cgaggagagg gggcacctgc ccgcttatct cgaaaacaac tttcgcgcgc
     5701 ttattcagca agaacaaaca gttagggatt ttcgcatgaa agccatccgt tttgccaaag
     5761 ccggcggccc cgaggttctc accctgcatg atgtcgagct gccgccgccc gcgcccggcc
     5821 aggtgcgtgt gcgccacacc gccatcggca tcaatttcct cgacacctat caccgctccg
     5881 gtctttaccc ggtcgccctg ccttcgggcc tggggatgga agcggccgga accgtcgaag
     5941 cgctgggcga aggcgtcgcc gagctgaaac tcggcgatcg ggtgggctat tgctccggcc
     6001 ccatcggcgc ttatgccgag gccaacaatg tcccggccga taaggtggtg aaactgcccg
     6061 ccacggtaag cgacgaaacc gccgccgcct ccctgctcaa gggcatgacg gcgcaatatc
     6121 tgctgaagcg tacctatgcg gtgaagcccg gccagaccat tctgattcat tcggccgccg
     6181 gcggcgtcgg cctgatcgcg ggccaatggg ccaagcatct gggcgccacc gtcctcggca
     6241 ccgtcggctc cgacgacaag atcgacctcg ccaaggccca tggctacgac caggtgctga
     6301 acctgcgcat ggacgattgg gtggcgcggg tccgcgaatt gacgaacggc gagggtgtgc
     6361 cggtggtcta cgattccgtc ggcaaagaca cgttcgccgg ctcactcgac tgtctggcgg
     6421 tgcgcggcat gctggtcagc ttcggcaatt cgtcgggtcc cgtgcccgcc ttcgcgccgg
     6481 ccatcttgag cgccaaaggg tcgctctatc tttcccgtcc gacgctgttc cactacacgc
     6541 gcacgccacg cgaactgcaa gagaccgccg ccgacctatt cgcgatgatc gcttcgggcg
     6601 cggtcaagat cgaaatcaac cagcgcttcg cgctcaagga cgcgcgcgcc gcccacgagg
     6661 ccctgcatgc gcgcaaaacc acgggcgcga cgatactgat tccgtgactt atttttgccg
     6721 gcgttctttg ctactcagcg gggtgagtca atctctcgaa tgctttaact gggactttat
     6781 gaccggcagc gacgtgcgat tcagacttct ggcttttgtc ttccgatttc cagctacgtg
     6841 ttcatgctct cgaagaactc ggcgttgttc ttggacgaac gcagcttgtc gagcagaaat
     6901 tcgatggcgt cgatggtgcc catgggcgcg aggatgcggc gcagtacgta agctttggcc
     6961 agcgtgccct tgccgacaag cagctcgtcc ttgcgggttc ccgaacgcag aatgtcgatg
     7021 gccgggaaga cgcgcttgtc ggcgactttg cggtcgagaa tgatttccga attgccggtg
     7081 cctttgaact cttcgaagat cacttcgtcc atgcggctgc cggtatcgat cagagcggtg
     7141 gcgatgatgg tgagcgaacc gccttcctcg atgttgcgcg cggcgccgaa gaagcgcttg
     7201 ggcttttgca gcgcgttggc gtcgacgccg ccggtgagga ccttgcccga cgacggcacg
     7261 acggtgttgt aggcgcggcc caggcgggta atggaatcga gcaggatcac cacgtcgcgc
     7321 ttgtgttcga ccaggcgctt ggccttctcg atcaccattt cggcgacctg gacgtggcgg
     7381 ctggccggtt cgtcgaaggt ggacgaaatc acttcgccct tcacctaacg ctgcatgtcg
     7441 gtcacttctt cgggccgctc gtcgatcaag agcacgatta aaaagcattc gggatgattg
     7501 gtggagatcg ccttggcgat gttctgcagg atcacggtct tgccggtgcg cggctgagcg
     7561 gtgatcagcg cgcgctggcc catgccctgc ggcgacacga tatcgatcac ccgtccggtc
     7621 ttgtccttga tggtggggtc ggccagctcc atcttgagcc agcgtgtcgg atagagcggc
     7681 gtcagattgt cgaagtggac cttgtgcttg acgttttccg ggtcctcgaa attgatggtg
     7741 gtgaccttga gcagcgcgaa atagcgctcg ccgtccttgg ggctgcggat cgggccttcc
     7801 accgtgtcgc cggtgcgcag cccgaagcgg cggatctgcg aaggcgaaac gtagatgtcg
     7861 tccggtccgg ccagatagtt ggattccggc gagcgcagga agccgaagcc gtcctgaagg
     7921 gtttccagca cacccgcgcc ggtgatctcc acattccgct cggcgagttc cttgaggatc
     7981 gcgaacatca tgtcctgctt gcgcatggac gaggcgttct cgatctccag ctcctcggcg
     8041 aacgacagaa ggtcggtggg ggatttggtc ttgagttcct gcagcttcat cgcctgcagg
     8101 ccggtttctt gcactgtcat ggggagttac acctggtacg acgctggaga tcgtctgttg
     8161 cccgcaagcc ccgcttcgtg cgcggtccgg cgagatcgtt tatggaggtc gaatcggtcc
     8221 gcccgcggcc gttccggctg acacggaatt cgcgctgacc ggcgcgtacc gcttgcgggc
     8281 ttcaatgggg aactgccgtc ttataccgcg atttggagcc tttgcaaact gcccgccgcc
     8341 gaattcgggc cggaaacgct aaatcggctt aacgatggca agaacaacga tcccgatcat
     8401 cagcacggtc gggacttcgt tccatacctt gaagaaccgc gccgaccgct ggttccggcc
     8461 cagcgcgaaa tccttggccc agatggcata cgaaaagtgt gtcaaggtca tggcagccac
     8521 cagcgcaagc ttcatttcga accagtgcag cgaccaatag tccatcagcc aggccatcgt
     8581 caggcccaat agccacaccg tgatcacggc cggcgccatg atgatcttca aaagcttgcg
     8641 ctccatgacc atgaagcgtt catagtcggc ggcgcccggc ttggtctggg tgtggtaaac
     8701 gaacagccgc ggcaggtaga acatcccgct catccaggcg atgacggcga tcacatgcag
     8761 cgctttgatc accagatagt ggtcgagaag aaattctttc atcccctaat tccgtccatg
     8821 cccgcacctt ccctgcgccg ccgggcagag ccggccgccg gcgctttccc gcgggtgcgc
     8881 caaactttgc cgcaccagtc tggcgaggcc tgaaataaat tcaggccgcg tgcttacggt
     8941 tggtacgcga agataatcgc gaacgccagc ctggcgcgcc aaatcgccat attccatgtc
     9001 gagttcgacc agggtttcgg aatgctccga gacaaacgag atcggcacca cgatggcacc
     9061 tttgccgtcg gcgccggcgc ggcggatttc agcgtccgtt gcgggcgtga tccatttcac
     9121 cggtccgacg cggctctggt agcagaccgt ccagtcggcc acccccaatc gttccacaag
     9181 ggcggcggcg gtttgttcaa cctgccagcg ataaggatcg cccttggcga caatccgttc
     9241 cggcaggccg tgcgccgaca acagcaaacg atacgagagt ccaggttgag tcttggccat
     9301 agcctccagc gtcaatcccg ccatggcctc gatgaagccg ggcaggcgcg gataacagca
     9361 ggggccagat tgaggcgcgg taaggcctgc cgccgaagcc gcccggcgcc aatccgtcag
     9421 cgccgacccg gtcgtggtgg tcgagaactg ggggtatagc ggcaagagca cgatgtgctc
     9481 ggcaccccac gccttgatcg cgcgcgccgc ttcatcgctg aacggcttcc agcagcgcat
     9541 ggcgatgaag gcccgcgcct catgctcgtc cagcgctttt tccaaggcgc gggcctgggc
     9601 ctgcgtttct tccagaatgg gagaccggcc gccgatcttg gcgtagatcg cgcgcgcgat
     9661 cggggcgcgg cgcttggcga tgaaccttgc caacggcttg cggacaaaag acggcagcga
     9721 caggatcgcc gggtcgccga acaggttgcg cagaaacggc tcgaccgctt ccggcgaatc
     9781 cgggccgccg agattgaaca ggacgacagc cagtttcacc gcgcgctccg caccagacgg
     9841 atcatctctt cgacatgctg ggtcggcgtt tccggcaaga ttccatgccc aagattgaaa
     9901 atgaacggaa cctcacccgc cgctttcaga atatgctcca cagcctccgc caaggcgctc
     9961 cctccggcca ccaatgcaac cggatcgaga ttgccttgca gcacgacttg cggagcaagc
    10021 gtccgcgccg cccagcccac aggcaccgcc gtgtcgagcg aaaccgcatc aacacccgtt
    10081 tccgcggtat agtgctcgta gcccttcaag gttgcggctc gcgggaagcc gatgatgcgc
    10141 gcctgcggca ccgccgcccg caccttggcc acaatcttct tggtaggctc gacgatccag
    10201 cgcgcaaacg cctgcggcgg caatcccgaa gcccagctat cgaagagctg aaccgcatcc
    10261 gcgcccgctt tcaattggcc gatcagatgc tgtgatacac attccgctaa aacatcgatc
    10321 aacttcgcga acccgaccgg atcgcggtaa gcccacagct ttgcggcttt ctgctcgtcg
    10381 ccgcccgcac cggccgccag atagctcgcc aaggtccacg gcgccccggc gaaacccagc
    10441 aacgcggtct gatctcccag acccgtccgc gttatccgca gggcttcata caccggctcc
    10501 aacattgcgc cccattgctc gggatcatcc atctgcccgg cgatagaagc gaccggcgac
    10561 agtcttggcc ccgcaccatc ctcgaatgtg acagacacgc cgagcgcagc aggtacgaca
    10621 aggatatcgg aaaaaatgat cgccgcatcg aagccaaaac gacgaaccgg ttgcagcgtc
    10681 acctcggcgg ccaattccgg cgtcaggcag agcttccaaa acgagcctgc cttgtttcta
    10741 gcctcacgat attcgggaag atagcgtccc gcctgccgca tcaaccagat aggcggtggt
    10801 gtcagaactt ttccattcag tgtttcaagg agcttcttac tgctctgcaa aacaagactc
    10861 cttacttaag gattatgtaa gtgatgtagg tcctgtgagt tgtggagtat ctcaagattg
    10921 tggatttcct ctaagatgga atatcaatgg actcaagagg ttcgaatagt ctcggagtag
    10981 ccggccctgt gtggggatga ggcggagaac ttggcctggt ctccacaggc aggcacaggc
    11041 gaaacgcaag ccgtgattct atccgccggt cgaggcacgg catgtatcga ttagggacga
    11101 aactgtgaac agagtttttc atctccatct ggtttcggat gcgacgggcg aaacgctcca
    11161 tgccgtcacc aaggcggcgc tcgcgcagtt cgagggcctg accgttcagg agcattccta
    11221 tacgctggtg cgcagcacgc gccaacttct gcgcgcgatc gaatatatcc gcgaccatcc
    11281 cggtcttgtc ctcttcacca tggttcatca agagctgcgt ctcgaactcg tcaatcaatg
    11341 caccttgatg aatgttccct atttcgacct catggacggg cccgtgagtc tgatgcggcg
    11401 gtttttcggc gccgaccaaa cctaccggcc gggggggcag cacgaaatcg atcaacacta
    11461 cttgcagcgg atcgaggccc tgaactacac gatccagcat gacgacggac aggtgctgga
    11521 cgatctggcg caagccgaag tgattctggc gggcgcaagc cgcacgtcca aaacgccgac
    11581 ctgtgtctat ctggcgatgc gcgggatacg ggccgcgaac gtgcctctcg ttctcggcct
    11641 gcccgccccg gcgcagcttc tcgcctgcac cgggccgctg atcgtcggcc tatgggcctc
    11701 gcccgagcgc ctggtgcaga ttcggcgcaa ccgtctggcg acgatcggcg aacaaaaact
    11761 gaccgattat gtcgatctgg aaaaagtgcg cagcgaggtg atgaagacgc ggcggctcta
    11821 tgaggagtat agctggccca ccatcgacgt gacgcggcgc tcggtcgaag aaaccgcggc
    11881 cacggttctc aatctgttgg ccgaacgccg gggcgaaaac ccgtgaacgc gctgatcctg
    11941 gcttccgcca gcgccgtgcg cgcccggctt ttgcgcgagg ccggcgtacc cttcgatgtc
    12001 cgcccggccg agatcgacga gggcgacatc aaagccttgc tggccgggaa gctgggggcg
    12061 gatgggattg ccggcgtctt ggccgagcgc aaggcgataa agatttccac tgctcaccca
    12121 caggcgctgg tgctgggatg cgaccagacc ctggccttcg acgggcggct gatcgacaaa
    12181 gcggcggatt tggcggcggc gcaggcgctg ctgcacgaaa tgcgcggcaa ggagcacagc
    12241 ctgtttacgg cgtgcgttct ggcgcaagac ggcgtccccg tctggcggcg gcttgagcat
    12301 agccggctat ggatgcggcc attcagcgat gcattcctgg acgattacct gcgaaccgag
    12361 ggaaacaagc tgctgggcag cgtgggctgc tatcatctgg aaggaagcgg catccagctt
    12421 ttcgagcggg tggaaggcga ctatttttcg gtgcttggcc tgccgctgat ccccctcttg
    12481 gcggcgctgc gcgagcatag gatgatcggg cgatgaacct cacgggcagc gcaaaacttg
    12541 ccggcgtggt gggctggccc atcgcgcatt cctt
//
LOCUS       DASWPU010000618         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_85334,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000618 DASWPU010000000
VERSION     DASWPU010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85334"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1345)
                     /locus_tag="VGC27_13205"
     CDS             complement(join(207..898,898..1345))
                     /locus_tag="VGC27_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611739.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY0283568.1"
                     /translation="MAVKKYVVRLSAEERAQLDELTRKGKRSAQLLTKARILQKADAS
                     ERGEGWSDSRIAAALDTSIATIERTRRQLVEEGFEAVLRRKYNPNSARPRIFDGAAEA
                     KLIALTLSPAPEGFARWSLRLLEEKVVELHIVERASDNTIGRTLKKTFLKPHRKQKWV
                     IPPDTSAAFVADMEDVLEVYQRPHDPQRPLVCLDETSKQLIIETRAPIPAKPGRKARH
                     DYEYERNGVANLFMVFAPLEGWRLVKVTDRHTAIDYAHMLRDLSDVHFPDAAKIALVQ
                     DNLNTHKPASLYEAFPAAEARRLVERFEWHYTPKHGSWLNMAESELGVLASQCLDRRI
                     ADKQILTHEVNAWQRHRNKHHAKANWQFKTADARVKLKRLYPQFG"
BASE COUNT          297 a          518 c          533 g          382 t
ORIGIN      
        1 cgcttgatga ggaagttgat cgccgtaacg ggtggccgtc gttgaaacgc gaaccgaacg
       61 ccgtgcgccg caaggttctc gcgggccgag ggttcattct ccttctctgc cttcgagggt
      121 aatgccgccc acgcatcaca aatcggagcc agaatgcgtt cgggctgatc gccgcaacaa
      181 taggatgcgg cgactagtcg cgcgagtcac ccgaactgag ggtacagtct cttcagcttg
      241 acgcgggcgt cggcggtttt gaattgccag ttggccttgg cgtggtgctt gttgcggtgg
      301 cgctgccatg cgttgacttc gtgggtgagg atttgcttgt cggcaatgcg gcgatccaga
      361 cactgcgacg cgagcacgcc gagttcggat tcggccatgt tgagccaact gccgtgcttg
      421 ggcgtatagt gccattcgaa acgctcgacg agccggcgcg cctcggcggc gggaaaggct
      481 tcgtagagcg aggcgggctt gtgggtgttg agattgtcct gcaccagcgc gatcttcgcc
      541 gcgtcgggaa aatgcacgtc cgacaaatcc ctgagcatat gggcataatc gatggcggta
      601 tggcggtcgg tcaccttcac aagccgccac ccttccagcg gcgcgaacac catgaacaga
      661 ttggcgacac cattgcgttc gtattcgtaa tcgtgccgcg ccttgcgtcc cggcttcgca
      721 gggatcggag cgcgcgtctc gatgatcagc tgcttcgagg tttcgtcgag gcacacgagc
      781 ggacgttgcg gatcgtgcgg cctctgatag acctccagca cgtcttccat gtcggcgaca
      841 aaggccgcgc tggtatccgg cggaatcacc catttctgct tgcgatgcgg tttgagaatg
      901 ttttttttag cgtccgcccg atcgtgttgt cgctggcgcg ctcgacaatg tgcagttcga
      961 cgaccttctc ctcgagcagg cgcaggctcc aacgggcgaa cccctccggg gccggagaga
     1021 gggtgagcgc gatcaatttt gcttctgctg cgccgtcgaa aatccgcggc cgcgcggaat
     1081 tgggattgta tttgcgtctc aacaccgcct cgaacccctc ttcgaccagc tggcgcctgg
     1141 tccgctcgat agtggcgatg ctggtgtcca gcgccgcggc aatccggctg tcgctccagc
     1201 cttcaccgcg ctccgatgcg tctgccttct gcaggatgcg ggccttcgtc agcagctggg
     1261 cggaacgctt gcctttgcgg gtcaactcgt cgagttgggc gcgttcctcc gcgcttaacc
     1321 gcaccacgta cttcttgacg gcaatctctt tcccgaccac gaatcacctc catcgctgcc
     1381 ttgcagcgac agtgattcag gggttgccgt cattttcaat gacgggagcg actaggcagc
     1441 gtggccatac acgacatcgc agcggcgtcc ggtcacgccg gtatccgggg cggtactctt
     1501 tcagagacta caacggcccc gcgccgcacc gaatgtgacc gagtgggtat tggcgtatcg
     1561 cttcgtccag gcaattcaac gcgctctcaa ggtcgctggc ttcgaagatc acagagcggt
     1621 ctttcttcga aatcagttcg atttcatatt tgctcatgtt ttctcgctac gccgctgaaa
     1681 cggcggacgc cttgatgtgg gtcaaagcta tccggccgcg agtccgcgaa
//
LOCUS       DASWPU010000619         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_60587,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000619 DASWPU010000000
VERSION     DASWPU010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60587"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VGC27_13210"
     CDS             complement(<1..712)
                     /locus_tag="VGC27_13210"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283569.1"
                     /translation="MALGQRLEMRQGQSLVMTPQLQQAIKLLQLSNVELAEYCEQELE
                     KNPLLERDESAPAGEAERAPAEREAPDEPLEASLAREDFSKCEEMDTSHDNLYDNDEP
                     AAVPALLPSAPLADWTTVKSSQRFEGDEDLLESTVAERGSLKDYLLEPLAIAALDPGK
                     RLICVSLIDAVDDAGYLRGDLGELAERLGTERSAVEEVVTVLQGFDPVGVGARDLAEC
                     LALQLKEKDRLDPAMRTML"
     gene            complement(730..1515)
                     /gene="lptB"
                     /locus_tag="VGC27_13215"
     CDS             complement(730..1515)
                     /gene="lptB"
                     /locus_tag="VGC27_13215"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY0283570.1"
                     /translation="MANETLSPAETKALRPRVLEGGKGLVVTRIGKSFNKRPVVRSVS
                     LSVKRGEVVGLLGPNGAGKTTCFYMITGLIPVDGGTIEVDGYDVTRLPMYRRARLGIG
                     YLPQEASIFRGLTAEENIRATAELVEPEPGLLEAAVEELLAEFGVTHVRNTPALALSG
                     GERRRVEIARALATHPSYILLDEPLAGIDPIAVGEIRNLVKHLKNRGVGVLITDHNVR
                     DALDIIDRAYILHDGQVMKEGTPQEIVADRDVRRVYLGERFSL"
     gene            complement(1556..2062)
                     /gene="lptA"
                     /locus_tag="VGC27_13220"
     CDS             complement(1556..2062)
                     /gene="lptA"
                     /locus_tag="VGC27_13220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY0283571.1"
                     /translation="MRELLALAVVFGLAAVAYAQQGGQAMIGMKDPNAPIEVTADNFD
                     ADANAKTAAYSGNVIVRQGDVRMRANVVRVAVVDGKPSKIFAQGRVVVDAPSGTATGD
                     NGVYDVGPRLVTLTGRVVLTRDKNVMRGSVLTVNLISGMATLDGGKQQGGRVQGLFTP
                     EAQKTGNP"
     gene            complement(2062..2769)
                     /gene="lptC"
                     /locus_tag="VGC27_13225"
     CDS             complement(2062..2769)
                     /gene="lptC"
                     /locus_tag="VGC27_13225"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY0283572.1"
                     /translation="MARDKFGREALAQPLAPPEAAADRPLRDWTDRKRSTALQALRYS
                     RFVGFMKRALPIGAVAILVAVIAYSVAPRHQDRFSLTYQQTGTLRNDLAMTKPRLSGT
                     DARGNPFVITAEAAVQDPANRRRATLKQVQADMQFDQQHWLNATAGTGLFDMDAGTLR
                     LDGGISLYTDSGYELHTNSADADLRNNIFRGDERVTGHGPLGSLSADSFQIDRVKRHL
                     KLSGRVHMIMYPQKAKR"
     gene            complement(2770..>3472)
                     /locus_tag="VGC27_13230"
     CDS             complement(2770..>3472)
                     /locus_tag="VGC27_13230"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0283573.1"
                     /translation="QDALLVLSWRGESAELSDLITHAKRFRIPLIGMASVPGSTLMQA
                     ADVQLLLPEVREACPMGLAPTTSTTLMLVLGDALAVALMERRGFSADQYRNFHPGGSL
                     GRALIRVSDLMHGADELPLAEENAPMRQALAAMQKCSFGCVGLTDKKGALVGIITDGD
                     LRRHWDRDLRNSKASEVMTRDPKVARSNQLAAEAIAFMNEKKITQLFVLDPKDKAKKP
                     VGILHIHDCLRAGLR"
BASE COUNT          567 a         1151 c         1118 g          636 t
ORIGIN      
        1 ccagcatcgt ccgcatggcc ggatcgagcc ggtctttttc cttcaattgc agcgccagac
       61 attcggccag atcgcgtgcg ccgacgccga ccggatcgaa gccttgcagc acggtcacga
      121 cttcctcgac ggcgctgcgc tcggtgccca gccgttcggc cagttcgccg aggtcgccgc
      181 gcagatagcc cgcgtcgtcg acggcgtcga tcagcgacac gcagatcagc cgcttgccgg
      241 ggtccagcgc cgcgatggcg agtggttcga gcaggtaatc cttcagcgac cccctttcgg
      301 cgacggtgga ttccagaagg tcttcgtcgc cctcgaaacg ctgcgaggat ttgaccgtcg
      361 tccaatcggc gagcggcgcg ctcggaagca aagcgggaac ggcggccggc tcgtcattat
      421 cgtagagatt gtcgtgcgac gtatccattt cctcgcactt ggagaaatcc tcgcgcgcca
      481 gcgaggcttc gagcggctcg tccggcgcct cgcgctccgc cggcgcgcgc tcggcttcgc
      541 cggcgggcgc gctttcgtcg cgttccagca gcggattctt ctccagctcc tgttcgcaat
      601 attcggcgag ttcgacgttc gacaattgca gcagcttgat cgcctgctgc aactgcgggg
      661 tcatgaccag ggactggccc tggcgcatct ccaatctttg acccagcgcc atgttcagcc
      721 tcccggcgcc tagagcgaaa accgctctcc gagatagacc cgccgcacgt cgcggtcggc
      781 gacgatctcc tgcggcgtgc cttccttcat gacctgaccg tcatgaagaa tgtaggcgcg
      841 gtcgatgatg tcgagcgcgt cgcggacatt gtggtcggtg atcagaacgc cgacgccgcg
      901 attcttcaga tgcttcacca gattgcggat ttcgcccacc gcgatggggt cgatgccggc
      961 cagcggctcg tcgagcagaa tgtacgaggg atgcgtcgcc agcgcgcgcg cgatctcgac
     1021 ccggcggcgt tcgccgcccg acagcgccag cgcgggcgtg ttgcgcacat gggtgacgcc
     1081 gaattccgcc agcagctctt ccaccgcggc ttcgagcaga cccggttcgg gctcgaccag
     1141 ctcggcggtg gcgcggatgt tctcctcggc ggtcaggccg cggaagatgg aagcctcttg
     1201 cggcagatag ccgatgccca gccgggcgcg gcggtacatg ggcaggcgcg tcacgtcgta
     1261 gccgtcaacc tcgatggtgc cgccgtccac ggggatcaaa ccggtgatca tgtagaagca
     1321 agtcgtcttg ccggcgccgt tgggaccgag cagacccacc acctcgccgc gcttgaccga
     1381 caggctgacc gaacgcacga ccgggcgctt gttgaagctc ttgccgatgc gggtgacgac
     1441 gagacccttg ccgccctcca aaacgcgcgg gcgcagggct tttgtttcgg ccggtgagag
     1501 ggtttcatta gccatgcgag aactgtgcca ccttgtgctt aaggaactgt gaaacctacg
     1561 gatttcctgt tttctgcgcc tcgggcgtaa acaatccctg aactcttcca ccctgctgct
     1621 tgccgccgtc cagtgtcgcc ataccgctga tcaggttgac ggtcaggacc gatccccgca
     1681 tcacgttctt gtcccgcgtc agcaccacgc gccccgtcag cgtcaccagg cgcgggccca
     1741 cgtcgtagac gccgttgtcg ccggtggccg tgccggacgg cgcatcgacc accacgcggc
     1801 cctgggcgaa aatcttgctc ggcttgccgt ccaccacggc gacccgcacc acgttggcgc
     1861 gcatgcgtac gtcgccctgg cggacgatga cgttgccgga ataggccgcg gtcttcgcat
     1921 tggcgtcggc gtcgaaattg tcggccgtga cttcgatcgg cgcgttggga tccttcatgc
     1981 cgatcatcgc ctgaccgccc tgctgggcat aggccacggc agcaagaccg aacactacgg
     2041 caagagccag caattcgcgc atcaacgctt cgccttctgc ggatacatga tcatgtgtac
     2101 gcggccgctc agcttcaaat gccgcttcac ccggtcgatt tggaaactgt cggcgctgag
     2161 cgagccaagc ggcccgtgtc ccgtcacacg ttcatcgccc cggaagatgt tgttcctgag
     2221 atcggcatcg gcgctgttgg tatgaagttc gtagccgctg tcggtgtaga gcgagatgcc
     2281 gccgtcgagc ctgagcgttc cggcatccat gtcgaacagg ccggtgccgg cggtggcgtt
     2341 gagccagtgc tgttggtcga actgcatatc ggcctggacc tgtttcagcg tagcgcgacg
     2401 gcggttggcg ggatcctgga cggccgcttc ggcggtgatg acgaaaggat tgcctctggc
     2461 gtcggtgccc gacagccgcg gcttcgtcat cgccagatcg ttgcgcagcg tgcccgtttg
     2521 ctgataggtg agcgaaaagc ggtcctggtg gcgcggagcg accgaatagg cgatcaccgc
     2581 gaccaggatc gccaccgccc caatcggcag cgcccgtttc atgaagccga cgaaacggct
     2641 gtagcgcagc gcctggagcg ccgtgctgcg cttgcggtcc gtccaatccc gcagcggacg
     2701 gtcggcggcg gcctcaggcg gagccaaagg ctgcgccagc gcttcgcgcc cgaatttgtc
     2761 gcgcgccatc tagcgcagcc ccgcgcgcag gcaatcgtgg atgtggagga taccgacggg
     2821 ctttttcgcc ttgtccttgg gatcgagcac gaaaagctgg gtgatcttct tttcgttcat
     2881 gaaggcgatc gcctcggcgg cgagctgatt ggagcgcgcg accttgggat cgcgcgtcat
     2941 cacctcgctc gccttgctgt tacgcagatc gcggtcccag tgccggcgca agtcgccgtc
     3001 ggtgatgatg ccgaccaacg cgcccttctt gtccgtcagg ccgacgcagc cgaagctgca
     3061 tttctgcatc gccgccagcg cctgccgcat cggcgcgttc tcttccgcca gcggcagttc
     3121 gtccgcgccg tgcatcaggt ccgaaacgcg gatcagcgcg cgtcccagcg agccgccggg
     3181 atggaagttg cgatactgat cggcggaaaa gccgcgccgc tccatcaacg ccaccgccag
     3241 cgcgtcgccc agaaccagca tcagcgtcgt cgaggtggtg ggggcaaggc ccatcgggca
     3301 cgcctcgcgc acttcgggca gcagaagctg gacatcggcc gcctgcatca gcgtggaccc
     3361 cggaacgctc gccatgccga tcagcggaat gcgaaagcgc ttggcatggg tgatgaggtc
     3421 ggaaagctcg gcggactcgc cgcgccagga cagcaccagc agcgcgtcct gc
//
LOCUS       DASWPU010000620         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_36606,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000620 DASWPU010000000
VERSION     DASWPU010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36606"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VGC27_13235"
     CDS             complement(<1..671)
                     /locus_tag="VGC27_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009586196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0283574.1"
                     /translation="MPRTTPLDKYRNIGIAAHIDAGKTTTTERILYYTGKSHKIGEVH
                     DGAATMDFMEQEQERGITITSAATTCYWRGHRINIIDTPGHVDFTIEVERSMRVLDGA
                     VAVFDAVAGVEPQSETVWRQADKYRVPRICFVNKMDRVGADFARCVQMMIDRLGSRPL
                     VITWPIGAEAGFKGIVDLVTMKALIWHEEQLGAKFDEVEIPANIADKVKELHTHLVEM
                     AVEED"
     gene            complement(682..1152)
                     /gene="rpsG"
                     /locus_tag="VGC27_13240"
     CDS             complement(682..1152)
                     /gene="rpsG"
                     /locus_tag="VGC27_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002006497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY0283575.1"
                     /translation="MARRRRADRREINPDAKFGDLVLAKFMNSLMYDGKKSAAEGIIY
                     GAFDTIQAKTKQDPLAVFHEALRNVSPALEVRSRRVGGATYQVPVEVRTDRSRALAIR
                     WIITAARGRNEPTMTGRLSGEILDAANNRGNAVKKREDTHKMAEANRAFSHYRW"
     gene            complement(1235..1606)
                     /gene="rpsL"
                     /locus_tag="VGC27_13245"
     CDS             complement(1235..1606)
                     /gene="rpsL"
                     /locus_tag="VGC27_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY0283576.1"
                     /translation="MPTINQLIRKPRGHKPKRNKVPALQHCPQKRAVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGYEALTYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGVL
                     DTQGVKDRRQRRSLYGAKRPK"
     gene            complement(2103..>2438)
                     /locus_tag="VGC27_13250"
     CDS             complement(2103..>2438)
                     /locus_tag="VGC27_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY0283577.1"
                     /translation="VMNYPKDIKAFYMRVNDDGRTVAAMDVLAPGIGEIIGGSQREER
                     LDVLDGRMTALGIDKEHYAWYRDLRRYGTVPHAGFGLGFERTLAYVTGLANVRDAIPF
                     PRTPGNAKY"
BASE COUNT          404 a          768 c          757 g          509 t
ORIGIN      
        1 tcgtcttctt cgaccgccat ctcgacaaga tgggtatgga gttccttgac cttatccgcg
       61 atgttcgcgg gaatttcgac ctcgtcgaat ttcgcgccga gctgctcttc gtgccagatc
      121 aaggccttca tcgtcacgag atcgacgata cccttgaacc cggcttccgc gccgatcggc
      181 caagtgatca ccagcggccg gctgcccagc cggtcgatca tcatctgaac gcaacgcgcg
      241 aaatccgctc cgacgcggtc catcttgttg acgaagcaaa tgcgcggcac ccggtacttg
      301 tcggcctggc gccagaccgt ctcggattgc ggctcgacgc cggccaccgc gtcgaacacg
      361 gcgacggcac cgtcgagcac gcgcatcgaa cgttccacct cgatggtgaa gtcgacatgg
      421 cccggcgtgt cgatgatgtt gatgcgatgc ccgcgccaat aacaggtcgt cgcggccgac
      481 gtgatggtga tgccgcgttc ctgctcctgc tccatgaaat ccatggtggc ggcgccgtcg
      541 tgcacttcgc cgatcttatg actcttgccc gtgtaataga gaatacgctc ggtcgtcgtc
      601 gtcttgccgg catcgatgtg cgcggcaatg ccgatgttac gatatttgtc gagcggggtc
      661 gttcggggca tcttgagtcc gttaccagcg ataatgcgag aaagcgcgat tggcttcggc
      721 catcttgtgc gtatcctcgc gcttcttgac cgcgttgccg cgattgttgg cggcgtcgag
      781 aatttcgccc gaaaggcgtc cggtcatcgt cggctcgttg cggccgcgcg cggccgtgat
      841 gatccagcgg atcgccagcg cgcgcgaacg gtcggtgcgg acttccaccg gcacctgata
      901 ggtggcgccg ccgacgcgcc gcgagcgcac ttccagcgcc gggctgacgt tgcgcagcgc
      961 ctcgtggaaa acggccagcg gatcctgctt ggtcttcgcc tgaatcgtgt cgaacgcgcc
     1021 gtagatgatg ccttcggcgg ccgacttctt gccgtcgtac atcaggctgt tcatgaattt
     1081 cgcgagcacg aggtcgccaa acttggcgtc cgggttgatt tcgcggcggt ctgcgcggcg
     1141 gcggcgggcc atatcgtagt tcctgtcttt ctctcttcct taactggatc ccggatcgtc
     1201 gcttcgcgcc gtccgggatg atcctaggct cggatcactt cggacgcttg gcgccgtaga
     1261 gcgaacggcg ttgcctgcgg tccttcacac cctgcgtgtc gagcacgcca cggatgatgt
     1321 ggtagcgcac gcccggaagg tccttcacgc ggccgccgcg gatcagcacc acggagtgtt
     1381 cctggaggtt gtggccttcg ccgggaatgt aagtgagggc ctcatagccg ttcgtcagac
     1441 gcaccttcgc gaccttgcgc agggcggagt tcggcttctt cggcgtcgtc gtatagacgc
     1501 gcgtgcagac ggcgcgcttc tgcgggcaat gctgcagcgc aggcaccttg ttgcgcttgg
     1561 gcttgtggcc acgcggcttg cggatcagct ggttgatcgt cggcattcgt cttccattca
     1621 tttgcgcgca acgcgcgcgg gcctgattta cttcattgcg cgcaacacgc gcgggctgac
     1681 cgctcgcacc atgcgcgcgg caaaaacgaa atccgaagca ggacacggcc tgcctcggtc
     1741 gaaacgcgga ggactggggc ctcgcccaag tcacgcgatc acacgcaatt aactggcttt
     1801 ccgacagcgt ttccgcgaat tcacgcggac tgcctgcgtc tgaaaggtgg cgctatataa
     1861 gcggcgtggg atgggccgtc aatggcagaa atccccgcag attccgtgcg ttccggccaa
     1921 agggctgcga gtggttcgcg ccccgccacg accatcggaa cgcgctttca gaagggatgg
     1981 accaagaacc tcgccccagc ctccaacagg atggttgtga ttgtgtctta ttatcgcctt
     2041 ttgaggcaat aaaatgtctt tgaggccgac gcgatcgtta ggtgactgcg ttccaccccg
     2101 tttcagtact tcgcgttacc gggcgtgcgc gggaacggga tagcgtcgcg cacgttggcc
     2161 aagcccgtga cataggcaag ggtgcgctcg aagccgaggc cgaaacctgc gtgcggcacc
     2221 gtgccgtagc gccgcaggtc gcggtaccaa gcgtaatgct ccttgtcgat gccgagggcc
     2281 gtcatgcgcc catccaggac atcgagccgc tcctcgcgct ggctgccgcc gatgatctcg
     2341 ccgatgcccg gagcaagcac atccatcgcc gccacggtcc tgccgtcgtc gttcacccgc
     2401 atgtagaacg ccttgatgtc cttcgggtag ttcatcac
//
LOCUS       DASWPU010000621         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_24250,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000621 DASWPU010000000
VERSION     DASWPU010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24250"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1285)
                     /locus_tag="VGC27_13255"
     CDS             complement(191..1285)
                     /locus_tag="VGC27_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEY0283578.1"
                     /translation="MEAFRECFSGVEDPRTGNARRHDLQELLVIALCTVLCGGESCVD
                     MADFAEEKEDFLREFLSLTNGLPSHDTFSRVFRALEPEQFRRCFQDFMVRFSETCQGV
                     IAIDGKVLRRSFDTASAKSSLHMVSAWGCEQRLVLGQIATDAKSNEITAVPKLLEMLS
                     LTGCVVTVDALNCQRDIAKQIVDQGGDYALALKGNQGTLHDDVRRFLDDPATTEITTE
                     TSVDADHGRIETRASTISDAIDWLQDAHHWPGLKAIGKIVRTRETGAKTTTETAYYLL
                     STPMTAERFGQVVRAHWAVENSLHWTLDVTMNEDQARNRLDNGPNNLAVLRHMALNLL
                     NAKKSKISNRRKIKRAGWSNAFLANLLAQI"
BASE COUNT          310 a          491 c          444 g          339 t
ORIGIN      
        1 catttccgcc acttcgtggc ggaggcgggc acggaggccc gccccactaa aaggcatttt
       61 ttcacaggct cggacgcccg cgctcccgat gacgcctcgg gtgccaagtg ttcgagtctt
      121 tccactcggc ggacggccgt gccgggcgga tcgtgcccag ccgacacacg cgggccaggg
      181 caatcgcata tcagatttga gcgaggaggt tggcgaggaa tgcgttgctc catccggctc
      241 gcttgatttt ccgtcgattt gagattttgg attttttggc gtttaggagg tttaacgcca
      301 tgtgacggag gaccgccaga ttgttcggac cgttatcgag acgatttctg gcttggtctt
      361 cgttcatggt gacatcgagc gtccagtgca ggctgttctc gaccgcccag tgagcgcgga
      421 cgacttggcc gaagcgttca gccgtcatgg gtgtgctgag aagataatag gcggtttccg
      481 ttgttgtttt ggccccggtc tcgcgggtcc gcacgatttt gccgatcgct ttaagccccg
      541 gccaatgatg agcgtcctgg agccagtcga tcgcgtcgga gattgtggat gcccgagtct
      601 cgatgcggcc atggtcggca tcgacgcttg tctcggtggt gatttccgtt gtcgccggat
      661 cgtccagaaa cctccggaca tcgtcatgca gggtgccttg attacccttg agggccaaag
      721 cataatcgcc accctggtca acgatctgct tggcaatatc gcgctggcag ttgagagcat
      781 cgacggtcac gacgcagccc gtcaacgaca gcatctccaa aagtttggga accgccgtga
      841 tttcgttcga cttggcatcg gtcgcgatct ggccgagaac cagccgctgt tcgcagcccc
      901 aggcactgac catgtgcaac gacgatttgg cgctcgcagt gtcgaatgac cggcgcagaa
      961 ccttgccatc aatcgcgata acgccctgac aggtctcgct gaaacgcacc ataaagtcct
     1021 ggaagcagcg gcgaaactgc tcgggctcca gcgcccggaa cacccggctg aacgtatcgt
     1081 gcgacggcaa gccattggtc aaagacagaa actcccgaag aaaatcctcc ttctcctcgg
     1141 caaagtccgc catgtccacg caagactcac cgccgcacag aaccgtgcac agcgcgatta
     1201 ccagaagttc ctgaagatcg tgccgccgag cgttgccggt gcgcggatct tccaccccag
     1261 aaaaacattc ccgaaacgcc tccatccaat cccccgagcg ccatcacaac caagcctctc
     1321 gaagaatcca tttcagcccc atatgcaaga aaattccgta tgcgattccc ctgggtcggc
     1381 ctgcctggac gctcccaggg gcggtctttg cgtgctacta tccttccgtt tccacttgga
     1441 ttaatctcag cggccatgtc agacgaggct cctgcccccc tccccaccga tcgcggtgcg
     1501 gccgacagca cgcccggcgc ggttccacgc gtcgttttcg aacatgcttt tttcggcaag
     1561 ctcgaagacg ccttttttca gaag
//
LOCUS       DASWPU010000622        10470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_91096,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000622 DASWPU010000000
VERSION     DASWPU010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10470
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91096"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VGC27_13260"
     CDS             <1..566
                     /locus_tag="VGC27_13260"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0283579.1"
                     /translation="HPHPQFGGTMNNPLVYDLFHMFHGLGCTAVRFNFRGVGRSQGTF
                     DSGSGELSDAAAILDWMNTLYPNPSFVWVCGISFGAWIGMQLLMRRPEITGFVSIAPP
                     ASMYDFAFLAPCPASGLIVHGTKDLVVPEPDVQKLVDRLTAQKGIKITYDKIEGANHF
                     FDKHETEVVETVKAYVSNRMNKPHEGR"
     gene            597..1856
                     /gene="tyrS"
                     /locus_tag="VGC27_13265"
     CDS             597..1856
                     /gene="tyrS"
                     /locus_tag="VGC27_13265"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY0283580.1"
                     /translation="MTDAPQFRSEFLRTVVARGQYYACTEPEALDALFAKERVTAYIG
                     FDCTAPSLHVGSLVQIMTLRRLQQAGGKPIVLMGGGTTKVGDPSGRDETRQILTPAQI
                     EANKQSIFKIFERFLGFGDGPSDAVMIDNAEWLDKLEYIPFLRDVGKHFSINRMLTMD
                     SVKLRLERDQPLSFLEFNYMILQAYDFVELYRRTGCRLQCGGSDQWGNIVSGTDLGRR
                     VADADLFGLTSPLITTASGAKMGKTAAGAVWLNADLCSPYDYWQYWRNTEDADVGRFL
                     RLFTDLPEADIARLEALQGAELNEAKKVLATEAAALAHGREAANSAAETARRTFEEGA
                     IDEGLPTFEIARDKLEGIAVTALAQLAGLTSSASEARRFIEGGGLRLNDGAVKDVKAT
                     VSAADAGAKGFIKLSVGKKKHILVKPV"
     gene            complement(2118..5615)
                     /locus_tag="VGC27_13270"
     CDS             complement(2118..5615)
                     /locus_tag="VGC27_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY0283581.1"
                     /translation="MKLSDLYPWKLHLLLRRYVKAHHVSRAALIAAGIAAAILFFTIG
                     AAIRLFIGPVSLGPFAGTLANALAEALPGIAVKYDQAAVEWARDEGRVNLVILGTRIF
                     DVDGRIIAQAPKAEIGLAAKPFLHGKIVVKRIALVGVQLTLVRTKDGGLRLGVEKDKH
                     ESDILSRITDAINANSGRASSLESFAVRNARLAFFDETTGLFVVSPQAAFLLTTVGSS
                     LDVKLDAAVEISGRPAHVSGEFVFPPKHGKVRGTLSVTGFELGALAANSKTFAAVKDT
                     ALKLDFSTSFVTEGTHLLSADFGVDARGAFGLPGIKGEPVQVRDLRAIGRYDGASGRL
                     LIEDATIDSDKIKARLRGTVDLVAGEDGALSDVRANLRLDRITLALPDVFASPVPFQL
                     VDLRAGWRPATRELVLDHLGVNGKPLTLQVSGKLTLPEGQSPAIELKGTIAPIGVRDL
                     IRYWPVVAAEGARSWAEENMFAGTVGPIAFETHISAGALDQPALPADALSVTFAVNGG
                     EINYIKGLTHMTALQGSAKVTGASFVADVPTARIGPLIVTAAKFTIPDLNVDDEVGDI
                     TGRIQGAMPDILTLVNLPPLGYPSRFGVVPADTKGTTTLDLSVRVPMRKAVTVDEIKI
                     GIKAAVNGLVVALGPRTQLTDGTVDFAIDNNKLHATGTTGIGGSASRLALDWTEDFKS
                     ASVSTITTRIAVKGALDEIGRAGLGLHVKDFIKGPLGVNGTLTGRRGALKQANLTFDL
                     TPVTATFDLIGVNKPAGFPITARVVTDFGPHSVLAEATMRMSGQGTAVTATAKFDADG
                     RLALLQAPIVRIGQLNDFALNVTRGPAGVDIAVRGRSLDGSRLAGRGSDGDEEELDEP
                     FHISARLDRLMLREGVAISSFALDVTGVAGRPATMSLTGALSKTATVAGTITPADAGR
                     RAVFTAGDMGLLSKGLFGFNSMRGGKLSFEATLPGRASDTAPKEPNVPDFQGKAVLKD
                     FRIVNQPFLARLFTAGSLGGLANLMQGQGIVVDTLEVPLSSRNGVVSIREGRAAGPAI
                     GGTAEGYIDRPKNSIALKGSLVPLFGINSALGNIPLLGDLLTSKQGEGIIGMTYSVTG
                     NADEPSVSVNPLSALAPGILRRIFEGKIPNASQAPSNLPKPVVPTPAPPPSNAVRTPS
                     PKPATPPDSEPASQPPKGQ"
     gene            5746..6210
                     /locus_tag="VGC27_13275"
     CDS             5746..6210
                     /locus_tag="VGC27_13275"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0283582.1"
                     /translation="MDLQPGDKAPAFKLATDGGGEVSLSKLKGRPFVLYFYPKDSTSG
                     CTQEAIEFTRLSKKFSELGVSLIAVSKDSIASHDKFKASYKLNLVLASDLDTKIAQAY
                     GVWGEKSLYGRKYMGMERATFLVDAKGKIRQIWRKVKVSGHAEAVLAAAKSL"
     gene            6351..7799
                     /locus_tag="VGC27_13280"
     CDS             6351..7799
                     /locus_tag="VGC27_13280"
                     /inference="COORDINATES: protein motif:HMM:NF039598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY0283583.1"
                     /translation="MTESLVERVWVWLHETFPERQVYIRSDGRVQFFTFGPTLQATLA
                     GLSLIFLGWVAFSTVNVIFKDRIIAAKDHRYQQMQSAYENRVADLQLSYDEANGALVS
                     AEDNFKSIADELQAKQNTIIHFLDRKRQVDATLMSGGMPASAKEADASTLENAPASDS
                     LGVAPPLTGMGGSAADDDDGGSSELTVSPQPVKPQPRAAKPTHAFMDIGGAVGRIAGA
                     IFGKRQPNAIPDATFARHPVLRRLAAETERVRRLSASETILMARAQEEIVEGVREINA
                     VLRRTGINPNQFAQRIESIEGVGGPEIPLQSVHIKGIADEAFQGAFLRASAVLGQLNQ
                     LLMGMRHVPLATPVHGSEFERTSGFGARIDPFTGRYAFHPGIDFAGPWGSAVRSTAPG
                     KVVWAGPRGTYGKMVEIDHGFGIHTRYGHLASILVQVGAKVTKGVPVGKLGSTGRSTG
                     PHVHYEVWYDDVVKNPRNFIEAGRYVLQQKQR"
     gene            7858..8349
                     /locus_tag="VGC27_13285"
     CDS             7858..8349
                     /locus_tag="VGC27_13285"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY0283584.1"
                     /translation="MRTSAAPSIISADLTVTGTLTSTGDIQIDGRVEGDVQSAGLVIG
                     DKAFIHGDVMAEEVTIRGRVQGSVRARKVLLASTCHVEGNILHEAFAVETGAFFEGNC
                     RHADNPLAEEAPRKTSEFRPAGAVAAVHPSTPAPSAVSSMVSRPMATSAATAATFTPL
                     KTN"
     gene            complement(8566..9633)
                     /gene="prfB"
                     /locus_tag="VGC27_13290"
     CDS             complement(8566..9633)
                     /gene="prfB"
                     /locus_tag="VGC27_13290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY0283585.1"
                     /translation="MFDWDKSVRRLDELNAKAEDASIWNNPQEAQKLMRERQRLEEGL
                     SSVRKVEAELADAQGLIEMAQAENDAAMVAEAEKSVAALARRSDQLRLESMLSGEADA
                     NDTYLEIHAGAGGTESQDWAEMLLRMYMRWAEKKGYKVDFLEESEGEGAGIKSATVLV
                     KGPNAYGWLKSEAGVHRLVRISPFDSNARRHTSFSSVTVYPVVDQTIEIDIPEKEVRI
                     DVYRSSGAGGQHVNKTESAVRITHLPTGIAVSCQTERSQHQNRAICWDMLRSRLYEIE
                     LAKKQAETGAFIGEKTDIGWGHQIRSYVLQPYQLVKDLRTGVESRSPDDVLDGDLDPF
                     MASALAQAGGGKAKEKIEDLE"
     gene            complement(9891..>10470)
                     /locus_tag="VGC27_13295"
     CDS             complement(9891..>10470)
                     /locus_tag="VGC27_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0283586.1"
                     /translation="GCSDSSWHGQSEPMLADTREQIIDPRTAYQIVSMLQGVVEYGTG
                     RSVASVGKPVAGKTGTSNDSKDAWFVGFSPDLACGVYVGFDNPRTLGKMEQGATVAAP
                     IFRDFMKGALAEVPPTPFRVPPGIEFVSVDRLTGTPVLVGTPGSIMEAFKSGTAPGDP
                     GAPPQLVIGGDTPSGPDAQSPNDVGEGTGGLY"
BASE COUNT         1964 a         3428 c         3204 g         1874 t
ORIGIN      
        1 tgcaccccca tccgcagttt ggcggcacga tgaacaaccc gctggtctac gacctgttcc
       61 acatgttcca cggactgggc tgcaccgccg tgcgcttcaa cttccgcggc gtgggccgca
      121 gccaggggac attcgattcg ggctccggcg agttgtccga cgccgccgca atcctcgact
      181 ggatgaacac gctctatcct aatccctcct tcgtctgggt ctgcggcatt tccttcggcg
      241 cctggatcgg catgcagctt ctgatgcgca ggcccgagat caccggcttc gtctccatcg
      301 ctccgcccgc gagcatgtac gatttcgctt tcctggcgcc gtgcccggct tccggcctga
      361 tcgtccacgg caccaaggac ctggtggtgc ccgagcccga cgtgcagaag ctggtcgacc
      421 ggctgaccgc gcaaaagggc atcaagatca cctacgacaa gatcgaaggc gccaaccact
      481 ttttcgacaa gcacgaaacc gaggtggtgg agaccgtgaa ggcgtacgtc tccaaccgga
      541 tgaacaaacc gcacgaagga cggtaactcc ccgccctttc cgctccgagt tgaagcatga
      601 cagacgcgcc gcaattccgc tccgaattcc tccgcaccgt ggtggcccgc ggccagtatt
      661 acgcctgcac cgagccggag gcgctggacg cactgttcgc caaggagcgc gtcacggctt
      721 atatcggctt cgactgcacg gcgccgagcc tgcatgtggg aagcctggtc cagatcatga
      781 ccctgcgccg gctgcaacag gcaggcggca agcccatcgt gctgatgggc ggaggcacca
      841 ccaaggtcgg cgacccctcc ggccgggacg aaacgcgcca gatcctgacc ccggcgcaga
      901 tcgaggccaa caagcagagc atcttcaaaa tcttcgagcg cttcctcggc ttcggcgacg
      961 ggccttcgga cgcggtgatg atcgacaacg ccgagtggct cgacaagctc gaatacatcc
     1021 cgttcctgcg cgacgtcggc aagcactttt cgatcaaccg catgctgacc atggacagcg
     1081 tgaagctgcg cctggagcgc gaccagccgc tgtcgttcct cgaattcaac tacatgatcc
     1141 tgcaagccta cgacttcgtg gagctgtacc ggcgcaccgg ctgccggctg caatgcggcg
     1201 gctcggacca atggggcaat atcgtcagcg gcaccgatct gggacggcgc gtggcggatg
     1261 cggacctgtt cgggctgacc tcgccgctga tcacgacggc gtcgggcgcg aagatgggca
     1321 agaccgccgc gggcgcggtg tggctcaacg ccgacctgtg cagcccctac gattactggc
     1381 aatactggcg caacaccgag gacgccgacg tcggccgctt cctgcgcctg ttcaccgacc
     1441 tgccggaagc ggacattgcg cggctggaag cgctgcaagg cgctgagttg aacgaggcca
     1501 agaaagtact ggcgacggaa gccgccgcct tggcgcatgg gcgcgaagcg gcgaacagcg
     1561 cggccgaaac ggcgcggcgc accttcgagg aaggcgccat cgacgaaggc ttgccgacct
     1621 tcgaaatcgc acgcgacaaa ctggagggga ttgcggtcac tgcgctggcg caactggccg
     1681 gattgacctc gtcggccagc gaagcgcgcc gcttcatcga aggcggcgga ctgcgcctca
     1741 acgacggcgc cgtgaaggat gtgaaggcga cggtgagcgc ggccgatgcc ggcgccaaag
     1801 gcttcatcaa gctctcggtc ggcaagaaga aacatattct ggtcaagccg gtttagggca
     1861 ataccctttc tgctttcggc gcgctaccac accttgagga ctctaaatcc atctccacaa
     1921 cccgttgcca ttcgtttttc agagttcggc cccctccccc cttatccagg gtgcctccat
     1981 ccgcatcttc cgtatcctct tcatccatcg ccaacaatcc cggcagccat catcataagc
     2041 ccgcccggcg aaccatggat aaggaacgaa gcgcaagtgc gactggaggg ccagcttaga
     2101 acagatcttt tctgccgtca ctgccccttc ggcggctgcg atgccggttc gctgtcaggc
     2161 ggagttgccg gcttgggtga gggagtccga acagcattgg agggcggcgg cgcaggggtc
     2221 ggcaccaccg gcttgggcaa gttcgacggc gcctgggagg cgttgggtat tttgccctcg
     2281 aaaatgcggc gcaggattcc cggcgccagc gccgaaagcg gattgacgct gaccgatggc
     2341 tcgtcggcat tgccggtgac gctgtaggtc atgccgatga tgccttcgcc ctgcttcgag
     2401 gtgagcagat cgcccagaag cggaatgttg cccagcgcac tgttgatccc gaacaacggc
     2461 accagcgaac ccttcagcgc gatgctgttc ttgggccggt cgatatagcc ctcggcggtg
     2521 ccgccgatgg ccggtcccgc ggcgcggcct tcgcgaatgg agaccacgcc gttgcgcgaa
     2581 gaaaggggaa cttccagcgt atcgacgaca atgccctgcc cttgcatcag attggccaac
     2641 ccgccgagcg aaccggcggt gaacaggcgc gccaggaacg gctggttgac gatgcggaaa
     2701 tccttcagaa ccgccttgcc ctggaaatca ggcacattcg gttccttcgg cgccgtgtcg
     2761 gacgccctgc cgggcagcgt cgcctcgaac gacagcttgc cgccgcgcat gctgttgaag
     2821 ccgaacaatc ccttgctgag caggcccatg tcgccggcgg tgaagacggc gcgccggccc
     2881 gcatccgcgg gcgtgatggt gcccgccacg gtcgcggtct tcgacagcgc gccggtcagc
     2941 gacatcgtcg cgggccggcc ggcaacgccg gtcacgtcga gcgcaaagga cgagatcgcg
     3001 accccttccc gcagcatcag gcggtcgagc ctggcgctga tatggaaggg ttcgtcaagc
     3061 tcctcctcgt cgccgtcgct gccgcgcccc gccaagcgcg aaccgtcgag cgaacgcccc
     3121 cgcaccgcga tatcgacgcc cgcggggccg cgcgtcacgt tgagcgcaaa atcgttcaac
     3181 tggccgatgc gcacgatggg cgcctgcaac aaggccaagc ggccgtcggc atcgaatttc
     3241 gcggtcgccg tcaccgccgt gccctgaccg gacatgcgca tggtcgcttc ggcgagcacg
     3301 ctgtgggggc cgaaatccgt caccacgcgc gccgtgatcg gaaatcccgc cggcttgttg
     3361 acgccgatca gatcgaaggt cgcggtcacc ggcgtcagat caaaggtgag gttggcctgc
     3421 ttcagcgcgc cgcggcggcc ggtcagcgtg ccgttgacgc cgagcggtcc cttgatgaaa
     3481 tccttgacgt gcaagcccag cccagcgcgg ccaatctcgt cgagcgcacc cttcacggcg
     3541 atcctggtgg tgatcgtgct cacgctcgcg gacttgaaat cctccgtcca atccagcgcc
     3601 aggcgcgacg ccgagccgcc gatccccgtc gtgcccgtgg catgcagctt gttgttgtcg
     3661 atggcgaaat cgaccgttcc gtcggtgagc tgcgtgcgcg gtccgagcgc cacgacaagt
     3721 ccgtttacgg ccgccttaat cccgatcttg atctcatcga cggtcactgc cttgcgcatc
     3781 ggcacgcgga ccgacaagtc aagcgtcgtg gttcccttgg tgtcggcggg gacgacgccg
     3841 aaccgcgacg gatagccgag cggcggcagg ttcacgagcg tcagaatgtc gggcatcgcc
     3901 ccctggatgc ggcccgtgat atcgccgacc tcgtcgtcca cgttgaggtc ggggatcgtg
     3961 aatttcgcgg cggtcacgat cagcggaccg atgcgcgccg tgggaacgtc cgcaacgaac
     4021 gaggcgccgg tcaccttggc cgacccttgc agcgccgtca tatgcgtgag gcccttgata
     4081 tagttgattt cgccgccgtt gaccgcgaac gtcacggaca atgcgtcggc cggcagtgcg
     4141 ggctgatcga gcgctccggc ggaaatatgc gtctcgaagg cgatcggccc caccgttccg
     4201 gcgaacatgt tctcttcggc ccagctgcgc gccccttctg ccgccaccac aggccagtaa
     4261 cggatcagat cgcgcacccc gatcggcgca atcgtgccct tgagttcgat ggcgggcgac
     4321 tggccctcgg gcagcgtcag cttgccggaa acctgcagcg tcagaggctt gccgttcacg
     4381 cccagatgat cgaggacaag ctcgcgcgtg gccggccgcc agccggcgcg cagatcgaca
     4441 agctggaacg gaaccggact ggcgaaaaca tccggcaagg ccaaggtgat cctgtccagg
     4501 cgcaagttgg cgcgcacgtc cgatagggcg ccgtcctcgc cggcgacgag atcgaccgtg
     4561 ccccgcagac gcgccttgat cttgtcggag tcgatggtcg cgtcctcgat cagcaaccgt
     4621 ccgctggccc cgtcatagcg gccgatagcg cgcaggtcgc gcacctgcac gggctcaccc
     4681 ttgatgccgg gcaagccgaa tgccccgcgc gcatcgacgc cgaaatccgc cgacaacaga
     4741 tgcgtgccct cggtcacgaa cgacgtcgaa aaatcgagct tgagcgccgt atccttgacg
     4801 gcggcgaacg tcttgctgtt ggcggccagt gcgcccagtt cgaagcccgt aaccgaaagc
     4861 gtgcccctga ctttgccatg cttgggcgga aacacgaact cgcccgagac atgggccggg
     4921 cgcccggaga tttcgactgc ggcatcgagc ttgacgtcga ggctggatcc gaccgtcgtc
     4981 aaaagaaacg cggcttgcgg agagaccacg aacaaacccg tcgtctcgtc gaagaacgcc
     5041 agcctggcgt tgcggaccgc aaagctctcc agcgacgagg cgcggccgct gttggcattg
     5101 atcgcatcgg tgatgcggct caagatgtcg ctttcgtgct tgtccttctc cacgcccagc
     5161 cgcaggccgc catccttggt gcgcaccagc gtcaactgca cgccgacgag cgcaatgcgt
     5221 ttgacgacga tcttgccgtg taggaacggc ttggccgcga ggccgatttc cgccttggga
     5281 gcctgggcga tgatccggcc gtcaacgtcg aatatccggg tgcccaggat gaccagattc
     5341 accctgccct cgtcgcgggc ccactcgacc gcggcctggt cgtatttgac ggcgatcccg
     5401 ggcagcgcct cggcgagcgc attcgccagc gtgcctgcaa aagggccaag cgacaccgga
     5461 cctataaaca ggcgtattgc cgcaccgata gtgaagaaca ggatcgcagc agcaattccg
     5521 gccgcaatca gcgccgcgcg actgacatga tgggctttta cgtaccgccg cagcagcaga
     5581 tgcaatttcc acgggtatag gtcggacaat ttcaaggtct gtacgcctcg tccacccacc
     5641 caatggccag ccaatcaggc gcaatcgggg tcttgcgctg aatcgcgcat cgctttatcg
     5701 cctatacggc cagacgttcg actgcaactt actttggaaa caggtatgga cctacagcct
     5761 ggcgacaaag cccccgcgtt caagctcgca accgatggtg ggggcgaggt ttccctatcc
     5821 aaattgaagg ggcgcccctt cgttctctat ttctatccca aggatagcac ctccggttgt
     5881 acgcaggagg ccatcgagtt cacaagacta tctaagaaat tcagcgagtt aggggttagt
     5941 ctgatcgctg tgtccaagga tagcattgcc tcgcacgaca aattcaaagc cagttacaag
     6001 ttgaatctgg tcctggcgtc cgacctcgac accaaaatcg cccaagccta tggcgtgtgg
     6061 ggcgaaaaaa gcctgtatgg aaggaaatac atgggcatgg agcgtgcgac cttccttgtg
     6121 gatgcgaaag gcaagattcg ccagatttgg cgcaaagtga aagtctccgg acatgcggag
     6181 gccgtgctcg ccgcggcgaa gtccctttga agccgccgcg acctttccgt cctcggttaa
     6241 ccgcaacgtc atttttctgg gctaagagct tgattgatcg ccctcaattg agtgaaatgc
     6301 gcctgactgg ggtaggcgta agcatagacg ccaaaaaggg aagtccggag atgacggaat
     6361 cgctcgtgga gcgcgtctgg gtatggcttc acgaaacatt tcctgaacgt caagtttata
     6421 ttcggagcga tggccgggtg cagttcttca ccttcgggcc cactctgcag gcgacgcttg
     6481 ccggtctctc gctcattttc ctcggctggg tagccttttc gaccgtaaac gtcatcttca
     6541 aggaccgcat tatcgcggcc aaggaccacc gctatcaaca aatgcagtcg gcttatgaga
     6601 atcgggtcgc cgatctgcaa ctgtcctacg acgaagccaa cggcgctctg gtttcggccg
     6661 aagacaactt caaatcgata gcggacgagt tgcaggccaa acagaacacg atcatccatt
     6721 tcctcgaccg caaacggcag gtcgatgcca cgctgatgag cggcggaatg cccgcaagcg
     6781 cgaaggaagc cgacgcctcc acactggaga acgcgccggc cagcgacagt ctgggcgtcg
     6841 cgccgccgct caccgggatg ggcggctccg cggccgacga cgatgacgga ggttcttccg
     6901 aactgaccgt cagtccgcag ccggtcaagc cgcagcctcg cgccgccaag ccgacgcatg
     6961 ctttcatgga tatcggcggc gccgtcgggc gcatcgccgg agcgatcttc ggcaaacgcc
     7021 agccgaacgc cattcccgac gcgaccttcg cccgccatcc cgtgctcagg cgcttggcgg
     7081 ccgaaaccga acgcgtccgc cgcctcagcg ccagcgaaac catcttgatg gcgcgggcgc
     7141 aggaagaaat cgtcgaaggc gtgagagaga tcaacgccgt cctgcgccgc accggcatca
     7201 atcccaacca gttcgcccaa cgcatcgaga gtatcgaggg tgtcggcgga ccggaaattc
     7261 ccctgcaatc cgtgcatatc aaggggatcg ccgacgaagc cttccagggt gccttcctgc
     7321 gcgcttccgc cgtgctcggc cagttgaacc agcttctcat gggcatgcgg catgtgccct
     7381 tggccacgcc ggttcacggc tcggaattcg agcgcaccag cggcttcggc gcgcgcatcg
     7441 atccgtttac cggccgttat gccttccacc cgggcatcga ttttgccggg ccctggggct
     7501 ccgccgtgcg ctctaccgcg ccgggtaaag tggtctgggc ggggcccaga ggaacgtacg
     7561 gtaaaatggt tgaaattgac catggtttcg gaattcatac gcgatacgga catctggcat
     7621 cgatcttggt gcaagttggc gctaaggtga cgaaaggcgt tccagtcgga aaactcggat
     7681 cgacggggcg cagcaccggg ccgcacgtgc attacgaggt ctggtacgac gacgttgtga
     7741 aaaacccgag aaacttcatc gaggcaggcc gctatgttct ccagcaaaaa caaagataag
     7801 gatacagctc ccgtgccccc ctcctctgct ccgggcactc tgcaatccaa gcgcctagtg
     7861 cgtacgtcgg ccgcaccatc gatcatcagc gccgatctca ccgtgaccgg cacgctgacc
     7921 tccacaggcg atatccagat cgacggccgc gtcgaaggcg acgtgcagag cgccggcctg
     7981 gtgatcggcg acaaggcatt catccatggc gacgtgatgg cggaagaagt gacgatccgc
     8041 ggccgcgtgc aaggcagcgt ccgtgcccgc aaagttctgc tcgcctccac ctgccatgtc
     8101 gaaggcaaca tcctgcacga agcctttgcg gtcgaaaccg gcgcgttctt cgaaggcaat
     8161 tgccgccacg cggacaatcc gctggccgaa gaagcaccga ggaagacttc cgaattccgc
     8221 ccggcgggcg cggtcgcagc cgtgcatccg tccacaccgg cgccgtccgc ggtttcctcg
     8281 atggtgtccc ggccgatggc gaccagcgcc gcaaccgccg cgaccttcac accgctcaaa
     8341 accaattagc cgatcgagct tccggtctgc caaaccccgc ttcgagaaat cgaggcgggg
     8401 tttttgcttg tggccgggct cgacccggcc atccagcaat aaaataaaag tcttccggcg
     8461 agatgacgcg cgcactggat ggtcgcctca agagcttgca ctccggccgc gtttcgcgga
     8521 ccggggaggc ggccatgacg aaaagatgat ttgcgaaccg gagctttatt ccaaatcttc
     8581 gatcttctct ttcgccttgc cgccgccggc ctgggccaag gccgacgcca tgaagggatc
     8641 gaggtcgccg tcgagcacgt cgtccggcga acggctttcc acgcccgtgc gcagatcctt
     8701 caccaactga tagggctgca gaacgtagga acggatctga tggccccagc cgatatcggt
     8761 cttctcgccg atgaaggcgc cggtctccgc ctgcttcttt gccagctcga tctcgtaaag
     8821 acgtgaacgc agcatgtccc agcagatcgc gcggttctga tgctgcgaac gctcggtctg
     8881 gcacgacact gcgatgccgg tcggcaaatg ggtgatgcgc accgcgcttt cggtcttgtt
     8941 gacatgctgg ccgcccgcgc ccgaggagcg atagacgtcg atgcgcacct ccttttcggg
     9001 aatgtcgatc tcgatggtct ggtccaccac cggatagacc gtcacggagg aaaagctggt
     9061 gtgccgccgc gcgttggaat cgaagggcga gatgcgcacc agccggtgta cgcccgcctc
     9121 gctcttcagc cagccatagg cgttaggccc cttgaccaga accgtcgccg atttgatccc
     9181 cgcgccctcg ccttcgcttt cttccagaaa atcgaccttg tagcctttct tctcggccca
     9241 gcgcatatac atgcgcagca gcatctcggc ccaatcctga ctttcggtgc cgcccgcgcc
     9301 cgcatggatt tccagataag tatcgttggc gtccgcctcg cccgacagca tggactccag
     9361 ccgcaactgg tcggagcgcc tggccagcgc ggcgacggac ttctcggcct ccgccaccat
     9421 cgcggcgtcg ttctcggcct gcgccatttc gatcaggcct tgcgcgtccg ccaattcggc
     9481 ctcgaccttg cgcaccgacg aaagcccttc ctcaagccgc tgacgctcgc gcatcagctt
     9541 ctgggcctcc tgagggttgt tccagatgga ggcgtcttcg gctttggcgt tcagttcgtc
     9601 gaggcggcgg acggatttgt cccagtcaaa gatgcctcct cagcagggcg acggcctgct
     9661 cgatgtcggc aatgttgcgt tcggtttccg ggcgcatatt cggtgattcc tgtttgcctt
     9721 gtgcctgtcg ttacttgccc acatccccgc cctccccttg agggagggtc gaaaaattca
     9781 agcgcagcga gaaatttttc ggggaggggt tcggcggcgg cgattcctac ccctccccga
     9841 attgcttcgc aattcgaccc tccctcaagg ggagggtaaa ggcaaccggt tcagtacaac
     9901 ccgccggtgc cttcgccgac atcgttgggc gattgggcat cggggccgga cggggtgtcg
     9961 ccgccgatca caagctgcgg cggcgcgccg ggatcgccgg gcgcggttcc cgatttgaag
    10021 gcttccatga tcgatccggg cgtgcccacc agtaccggcg tgcccgtcaa ccggtcgact
    10081 gaaacgaact cgattccggg cggcacgcgg aacggcgtcg gcggcacctc ggccagcgcg
    10141 cccttcatga aatcgcggaa gatcggcgcg gcgaccgtgg cgccttgttc catcttgccc
    10201 aaggtgcgcg ggttgtcgaa gccgacatac acgccgcacg ccagatcagg cgagaagccc
    10261 acaaaccacg catccttgga gtcgttggag gttcccgtct tgccggccac cggcttgccg
    10321 acgctggcca ccgagcgccc ggtgccgtat tccacgacgc cctgcagcat cgaaacgatc
    10381 tgataggccg tgcgcggatc gatgatctgt tcgcgggtgt cggccagcat cggctcggac
    10441 tgcccgtgcc agctcgaatc gctacagccc
//
LOCUS       DASWPU010000623         4256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_91099,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000623 DASWPU010000000
VERSION     DASWPU010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91099"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VGC27_13300"
     CDS             <1..858
                     /locus_tag="VGC27_13300"
                     /inference="COORDINATES: protein motif:HMM:NF040149.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5700 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HEY0283587.1"
                     /translation="RLKQREASVGWPFTDAEFKDFLLSPETVQRRYALRNALMAWELL
                     PLEAIASQALEYLPAAARLDAKLFPVIKPRPNSFVHGTSDDAMVFINVDPALQTRQIE
                     ALIVHELHHIGLFPFECVDDVRQELRAPVRRAVGLMRNFREGYAMLAAAGGPDVHPRH
                     FDGAAARHEWDRAMAHFEADLKAIERFLFDVIHDRLTPEQAERLAAEFLGRQGPWYTV
                     GWRMAATVEQYFGRALLIDSMPDPRLLLLCYNSAAVQAASDRAADRFPIWSLDLMRTL
                     VPDRAGPAP"
     gene            complement(875..1525)
                     /locus_tag="VGC27_13305"
     CDS             complement(875..1525)
                     /locus_tag="VGC27_13305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0283588.1"
                     /translation="MSVGRLGKILDCTADAGFATNREGVIRVWNRSAERLLGYGASEA
                     LNTTCADLLSCHTNTSPHCKQCGVISQCILGSTIPNYDTKVRTASGALIWINVSIIVY
                     QNEQNDGDLLVVHLVRDITRQKEAAQLSQQLVELVRQIAQTSRIEERLAPILPLTEQE
                     HSILRDIGEGKHPTVVARERQITPGTLRNHLHKVNQKLGTRNRLEAVLEAKRRGVI"
     gene            complement(1810..1962)
                     /locus_tag="VGC27_13310"
                     /pseudo
     CDS             complement(1810..1962)
                     /locus_tag="VGC27_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2383..2576
                     /locus_tag="VGC27_13315"
                     /pseudo
     CDS             2383..2576
                     /locus_tag="VGC27_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047193.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
     gene            3011..3178
                     /locus_tag="VGC27_13320"
     CDS             3011..3178
                     /locus_tag="VGC27_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283589.1"
                     /translation="MLTRIRRQRFSKRKSIVTILKEVMTNEASVLMDSDAANKTLDAD
                     IAPLAAHLDTP"
     gene            3379..>4256
                     /gene="dusA"
                     /locus_tag="VGC27_13325"
     CDS             3379..>4256
                     /gene="dusA"
                     /locus_tag="VGC27_13325"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEY0283590.1"
                     /translation="MEWSERHCRMLHRLLSDHARLYTEMVTAQAAIRGRRERLLGYDA
                     REHPVALQLGGAEPAALAEAAKIGESFGYDEINLNAGCPSDRVQSGRFGACLMREPEL
                     VAECVAAMRATVSVPVTVKCRIGVDDQEPGEALRALIAACAAAGVDTFIVHARKAWLE
                     GLSPKENRDVPPLDVELVYAVKRENPALTIVLNGGITTLDVAEAHLNQVDGVMLGRAA
                     YQSPALLAEVDARFFNGAEHTVEAAVEAYLEYVERRLAEGVPLNAMTRHMLGLFNARP
                     GARLFRRHLSENATRPG"
BASE COUNT          809 a         1264 c         1320 g          863 t
ORIGIN      
        1 cggctgaaac agcgtgaagc ttccgtcggg tggccgttca ccgacgccga gttcaaggat
       61 ttcctgctct ctcccgaaac ggtgcagcga cgatatgcgc tgcggaacgc gctgatggca
      121 tgggaactgc tgccgctcga agcgatcgct tcgcaagcgc tcgagtacct gccggcggcc
      181 gcgcgcctcg atgcgaagct cttccccgtc atcaaaccgc ggccgaacag cttcgtgcac
      241 ggaacgagcg acgatgcaat ggttttcatc aatgtcgatc ctgccttgca gacgaggcag
      301 atcgaggccc tgattgtgca cgaactgcat cacattggtc tctttccatt cgagtgtgtc
      361 gatgacgtgc ggcaggagct gcgagcgccg gtgcggcggg ctgtcggatt gatgcggaac
      421 ttccgcgaag gttacgcaat gctcgccgca gctggcggac cggacgtgca tccgcgccac
      481 ttcgacgggg cggccgcgcg ccatgaatgg gatcgcgcga tggcgcactt cgaagccgac
      541 ctgaaagcga ttgagcgctt tctgttcgac gtcattcacg accggctcac gccggaacag
      601 gcggagcggc tcgcggccga attcctcggc cggcaaggtc cttggtatac ggtcggctgg
      661 cgtatggctg cgaccgtcga acagtacttc ggcagagccc tgctgatcga ctccatgccg
      721 gacccgcgcc ttctactgct ctgctataac tcggccgccg tccaagctgc atccgatcgg
      781 gccgccgatc gattcccgat ctggtcgctc gatctcatgc gcaccctcgt gccggaccgg
      841 gcaggaccgg cgccctgacc gtgcagccgg ccggtcagat gacgccgcgc cgcttggcct
      901 ccagcactgc ctccaatcga ttgcgcgtcc cgagcttctg attgaccttg tggaggtgat
      961 tgcgcagcgt gccgggcgtg atttgccttt cgcgggcaac caccgtcggg tgcttgccct
     1021 caccgatatc tctcaggatg ctgtgttcct gctcagtcag cggcaaaatc ggcgcgagcc
     1081 gttcctcgat acgggacgtc tgtgcaattt gccgcacgag ttcgacgagc tgttggctca
     1141 actgcgccgc ctccttctgc cgcgtaatgt cacgaaccag gtggacgacc aggagatcgc
     1201 cgtcgttttg ttcgttttga tagacgataa tggacacgtt gatccaaatg agcgccccgg
     1261 atgcggtcct cacctttgtg tcgtaatttg gaattgtaga accgagaatg cactgagaga
     1321 tgaccccgca ttgtttgcag tggggactgg tgttcgtgtg acaggaaagc agatcggcgc
     1381 aggtcgtgtt cagagcctcc gaagcgccgt agccgagcaa ccgctcggcg ctgcgattcc
     1441 aaacgcggat cacgccttcc cggttcgtgg cgaagccggc gtcggccgtg cagtcaagga
     1501 ttttccctaa acggccgacg ctcatgtctc gcaatctcta tccaaagatg agctgccggg
     1561 agcctgcatc taagggcgcg ggtaattcat tgatctagat caaccagaac atgtattcta
     1621 taaatattgc tacttaaaga cgaaacgtgg cgtggtaccg tttgcccatc atgcgggacg
     1681 agggactggg actagtaggt ccgcctgcgc tgattttgtt gaaaaatcga cctcgaacgt
     1741 acctgatctg acctgatatt ttggagcgga gtactgtgtc cggctcccgg cctcagattg
     1801 ccaaggtggt tgagggagcg ggatcagtat ggcgagcttt ttgaggtttg gggcggtggc
     1861 ggccaggagg aactcgtcgc gggtgcggca ggggcctcgc gatctgaggc gcacgagatg
     1921 aaggacgcgt ctcaagtgag cgaacagcat ctcgattttt tttgcgctcg cgctgtgact
     1981 aaaggcaaga tgttcgccgt ctttggtacc ccaaggcgcg agctgaagta caaaaccggc
     2041 gacacacgaa cgattacgaa gggtgcacga tggccgtcat gcgcaaaccc gaggacacgt
     2101 gtgtaaaacc gagactcgcc gccacatcat tgacggccag aatgatgtga tcgatctgcg
     2161 cggtcgtgga cggtccttcg tattggcagt cccagtctag cgcggcacac cggcaatttc
     2221 tttccttcgc caatctgcga cgccatctga tccttgttac taaacgcaga attcggttct
     2281 ttctactcga ccccaacaac gacgcgtcct tgcgtcgtta aattggggtc cgatgtgaac
     2341 gccatcgctt ttcgatccga aaccctcgcg cgcagcgcgc gcatgctgcg cacagcacta
     2401 ggtccagcta tcgcaagcta tcttgaagac ccgtcgatcg tggaagtcat gctcaatccc
     2461 gatgggtggc tttggatcga ccgtctatca ggagggctcg aagatactgg ctgtcatgtg
     2521 acgccgccga cgcggaacgc atcgtgcgtc tcgtcgccca tcatatcggc gtcgaggcgc
     2581 agcgcacccc ccggccgtta tccgcagagt gaattccccg atcccatctc gcgccttgtc
     2641 gaccaggagc gccgggcgta atttgaagaa gaaggctcac atttcgagag ccggtatttt
     2701 cttagtcttg tctggctgcc accggccgaa gacgcaacgt gcgtcgaaaa ctggctctat
     2761 gagggccgcg cgcatgaagg cgccgattgg cgcgcagcac tcatcggctt catcgaccgc
     2821 accgaccgtg tcctcgccct cttggacggc tttatgccgg aggtgggttg gctcgatgac
     2881 atcggcacgc tcaccatcat gggctttccg tcgcagacct gcccggcctg ctcgatgacc
     2941 ttaaccgtct cgcttttccc taccgctggt cgacacgcgc tatctgtctc acaagactga
     3001 tgcgaccagg gtgctgaccc gtatccgccg gcagcggttt tccaagcgca aatccatcgt
     3061 aacgatcctg aaggaggtga tgacgaatga agcgtcggtg ctgatggatt ccgatgccgc
     3121 caacaagacg cttgacgccg acatagcgcc ccttgcggcg cacttggaca cgccatgacc
     3181 ccggaaggga gtttcgtgaa ggtggccatt ttcgtgtacg cttcccgtgt gcaaaacgtg
     3241 tgcactgagg tgtcgaaacg tggcgaaaaa cgtcgtattt cgggatacaa aaaagtgcac
     3301 acgacgctca ctaacctttt gaagaatata caaataatgc aacaaaatca agcacataga
     3361 ttctgtattg cgccaatgat ggaatggagc gagcggcatt gccggatgct gcaccgcctg
     3421 ctcagcgatc acgcgcgtct ctacaccgag atggtgacgg cgcaagcggc gatccgcggc
     3481 cggcgcgagc ggctgctcgg ctacgacgcg cgcgaacatc cggtggcgtt gcagctcggc
     3541 ggcgccgaac cggccgcgct ggccgaagcc gcgaagatcg gtgagagctt cggttacgac
     3601 gagatcaacc tgaacgccgg ctgtccatcg gaccgggtgc agtccgggcg ctttggcgcc
     3661 tgcctgatgc gcgagccgga actggtggcg gagtgcgtgg cggctatgcg tgctacggtg
     3721 tcggtccccg tcacggtgaa atgccgcatc ggcgtggacg atcaggaacc gggcgaggcg
     3781 ctgcgcgcgt tgatcgccgc gtgcgccgct gcgggcgtgg atacgttcat cgtgcatgcg
     3841 cgcaaggcgt ggctggaagg cttgtcgccg aaggagaacc gcgatgtgcc gccgctcgat
     3901 gtcgaactcg tctatgcggt caagcgggag aatccggcgc tgaccatcgt gctcaacggc
     3961 ggcatcacga cgcttgatgt cgccgaggct cacctgaatc aggtggacgg cgtgatgctg
     4021 ggccgcgccg cttatcaatc gcccgcgctc ctggcggagg tggacgcgcg gttcttcaac
     4081 ggcgcggaac atacggtcga agcggcggtg gaggcttatc tcgaatatgt ggagcgccga
     4141 ttggccgagg gggtaccgct gaacgccatg acccggcaca tgctgggcct gttcaatgcg
     4201 cggcctggcg cgcgcctgtt ccgccgccat ctgagcgaga acgcgacgcg gcccgg
//
LOCUS       DASWPU010000624         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_72975,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000624 DASWPU010000000
VERSION     DASWPU010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72975"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..848
                     /gene="rplB"
                     /locus_tag="VGC27_13330"
     CDS             6..848
                     /gene="rplB"
                     /locus_tag="VGC27_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019297986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY0283591.1"
                     /translation="MALKQYRPTSPGQRQLVLIDRSGLHKGPPVKGLTEGQTRNGGRN
                     NTGRVTVRWQGGGHKQRYRIIDFKRLKFDIPATVERLEYDPNRTSFIALVKYKDGELA
                     YILAPQRLAVGDTVVSGDKVDVKPGNAMRLSAMPIGSIIHNIEMKPGKGGQIARSAGT
                     YAQYLGRDAGYAILRLNSGEVRRVRLECLATVGAVSNPDHMNEVISKAGRNVWKGKRP
                     HVRGTAMNPIDHPHGGGEGRTKGGRNPVTPWGKPTKGAKTRKNKRTTKYIVRSRHGKT
                     TQGQ"
     gene            850..1137
                     /gene="rpsS"
                     /locus_tag="VGC27_13335"
     CDS             850..1137
                     /gene="rpsS"
                     /locus_tag="VGC27_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY0283592.1"
                     /translation="MTRSVWKGPFVDGYLLKKAEVARGSGRKDVIKTWSRRSTILPQF
                     VGLTFGVYNGKKHIPVLVTEDMVGHKLGEFSPTRTFHGHSGGGDKKAKRAE"
     gene            1137..1502
                     /gene="rplV"
                     /locus_tag="VGC27_13340"
                     /pseudo
     CDS             1137..1502
                     /gene="rplV"
                     /locus_tag="VGC27_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            1646..>1863
                     /locus_tag="VGC27_13345"
     CDS             1646..>1863
                     /locus_tag="VGC27_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY0283593.1"
                     /translation="MGQKVNPTGLRLGINRTWDSRWYAGKKEYGKLLQEDIKIRNHLC
                     ERLKQAGVSRVVIERPHKKCRITIHSARP"
BASE COUNT          429 a          561 c          592 g          281 t
ORIGIN      
        1 gtaagatggc gctcaaacaa tatcggccta cctcgcccgg tcagcgccaa ttggtgctca
       61 tcgaccgttc cggcctgcac aaagggccgc cggtcaaagg gctcaccgaa ggccagacgc
      121 gcaacggagg ccgtaacaat acgggccgcg tcaccgtgcg atggcaaggc ggcggtcaca
      181 agcaacgcta tcgcatcatc gatttcaagc ggctcaagtt cgacatcccc gctacggtcg
      241 aacgtctgga atacgacccg aaccgcacct ctttcatcgc cctcgtaaaa tacaaggacg
      301 gcgagctggc ttacatcctg gcgccgcagc gcctggcggt cggcgacacg gtcgtttcgg
      361 gcgacaaggt ggacgtaaag ccgggcaacg ccatgaggct ttcggcgatg cccatcggca
      421 gcatcatcca caatatcgag atgaagccgg gcaagggcgg gcagatcgcg cgctcggccg
      481 gcacctatgc gcaatatctc ggccgcgacg ccggttacgc cattctgaga ctcaattcgg
      541 gcgaagtgcg ccgggtccgg ctggaatgcc tggcgacggt cggcgccgtg tcgaaccccg
      601 accacatgaa cgaggttatc agcaaagccg gccgcaacgt ctggaaaggc aagcgtccgc
      661 atgtgcgcgg cacggcgatg aacccgatcg atcatccgca cggcggcggc gagggccgca
      721 ccaagggcgg ccgcaatccg gtcacgccgt ggggcaagcc gaccaagggc gccaagacgc
      781 gcaagaacaa gcgcaccacg aagtacatcg tgcgttcgcg ccacggcaag acgacgcaag
      841 gacagtgata tgacacgttc ggtttggaaa ggtccctttg tcgacggtta tctgcttaag
      901 aaggcagagg tcgctcgtgg ctccggccgc aaggacgtga tcaaaacctg gtcgcgccgt
      961 tcgacgatcc tgccgcagtt cgtgggcctc acttttggcg tttacaacgg caagaaacac
     1021 atccccgtgc tcgtaacgga ggacatggtc ggccacaagc tcggcgagtt ctcgccgacg
     1081 cggacgttcc acggccactc cggcggcggc gacaaaaaag cgaagagggc ggaataatgg
     1141 gtaagaatgc acgcgcccgc gttcttgccg atcatcaggc caaggccatc ggccggatga
     1201 tccgcatttc tccgcggaag ctgaacctcg ttgccgagac aatccggggc aagaaggcgg
     1261 aggcggctct ttacgagctt actttctcgc cgaagcgtat tgccaagacg gtgaagaagg
     1321 tcttgcagtc cgcaatcgcc aatgcggaaa acaaccacga tctcgatgtc gacgatttgg
     1381 tcgtgagcga agccagcgtc ggcaagaaca tggtgttgaa gcgctttcat gcccgcgccc
     1441 gcggacgcgg cgcggggatt gagaagcctt tcagtcagat caccatcgtg gtcgaggaac
     1501 ggcgcgagac ggagccggag gccaaggccg agccggcgaa gcccgccgaa ggaaagacgg
     1561 tcggcaagcc gaagaagaag gcgcctgcga agaagaagca ggcggcgaag aagccagcgc
     1621 ccaagcgcgc gaaggagaat gcgtaatggg tcagaaggtc aatccgaccg gcttgcggct
     1681 tggcatcaac cgcacctggg attcgcgctg gtacgcgggc aagaaggaat acggcaagct
     1741 ccttcaggaa gacatcaaga tccgcaatca cctgtgcgaa cgcttgaagc aggcgggcgt
     1801 cagccgcgtc gtaatcgaac ggccgcacaa gaagtgccgg attacgatcc attccgcccg
     1861 tcc
//
LOCUS       DASWPU010000625         3510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_18072,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000625 DASWPU010000000
VERSION     DASWPU010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18072"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VGC27_13350"
     CDS             <1..541
                     /locus_tag="VGC27_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283594.1"
                     /translation="APARGPARGPARGGRFAPQPDPVSPAVKNSLLARAGGGGARKVK
                     ETELCRLLLQDPEIAVRQGETLAAIHFSDPLLDRLRQELLNLAASGFRLEKGGLENHL
                     VRAGMAELVERLLAPEAPAGQESANGEDARFLRAAAELREMAELAPERNRAVERFKSE
                     ATEENWLQVRRLLGGRSEE"
     gene            627..935
                     /locus_tag="VGC27_13355"
     CDS             627..935
                     /locus_tag="VGC27_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283595.1"
                     /translation="MAKKSVKKKKAVKKKAKLAPKRRAAKAAKARKPARKVAKKAVKT
                     SAKKAVKKTVRKPAPKKTKKRVKKAAPEVVAPLTDIGDLSGGASGLGEVSSPGIPKLF
                     "
     gene            1020..3413
                     /gene="rpoD"
                     /locus_tag="VGC27_13360"
     CDS             1020..3413
                     /gene="rpoD"
                     /locus_tag="VGC27_13360"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY0283596.1"
                     /translation="MATKAKKTAKPKAVKRPPAKAARAKSAPAKSVRTAAKSKPAPKP
                     AKAVAAKSSAKTQPKTQPKAKPELKSAAKVQAPAKSAAAVRVSVVPPVAAKVNGKPAV
                     PVKPGVKPGENESPGDPDSPLLDMSDVGVRKMIARAKTRGYVTYDELNKVLPSDKTSS
                     EQIEDTMSMLSEMGINVIESEEQEEGADAEVPALAEGKAVATTKEGAAEQYDRTDDPV
                     RMYLREMGSVELLSREGEIAIAKRIEAGRELMIGALCESPLTFEALTVWRDELNEGKV
                     LLRDIIDLEAMYGGGPDAQAAAAGVAGGPPVLPLVQMNGNAAAPPVADFRRPEPPKPA
                     AAPPPAAPKPEGAEGEELDPAEAQAKADAEAAEADDFDDEGNLPLSAMEAALKPQVLE
                     TLDNIAALYKKLGRLQDASVEAALASDELSSGQERRFKKLRHETVDLVKGLKLNNNRI
                     EALVDQMYGINRRLVSLEGKLLRLAEAHGVAREDFLKEHFGNELDPNWTRRVGRLSRM
                     GWKDFIAEDRDKVHPLRDEIQTLAQEMRQSIADFRRIVQTVQKGERESGVAKKEMIEA
                     NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMIETINKLVRTSRQMLHEIGREPTPEELAERLAMPLEKVRKVL
                     KIAKEPISLETPIGDEEDSHLGDFIEDKNAILPIDAAIQANLRETTTRVLATLTPREE
                     RVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDN"
BASE COUNT          757 a         1116 c         1148 g          489 t
ORIGIN      
        1 ggccccggcg cggggtcccg ctcggggtcc ggcgcggggc gggaggttcg cgccgcagcc
       61 cgatccggtg tccccggcag tgaagaacag cctgctcgcc cgtgccgggg gagggggcgc
      121 gcgcaaggtt aaggaaacgg agctttgccg gctcctgctc caggaccccg aaatcgccgt
      181 ccgccagggc gaaacccttg cagcgattca tttttccgat cctttgcttg acaggctgcg
      241 ccaggaactc ttaaacctcg ccgcctccgg ctttaggctt gaaaaagggg ggctggagaa
      301 ccatctcgtc cgtgcaggca tggccgaact cgtggagcgc ctattggcgc cggaggcgcc
      361 ggcgggccag gagagcgcaa atggcgagga tgcccgcttt ctgcgagccg ccgctgagct
      421 tcgcgagatg gcggaattgg ccccggaacg aaatcgcgcg gtcgagcgtt tcaaatccga
      481 ggcgacggag gagaattggc tccaagtccg gcgcctgctc ggcggccgct ccgaagaata
      541 gaacaggcgg tcaatagcga ttttgctccg tttaggttag gaacgcttcg ttgcgcgcgc
      601 aacggaccga aagatgaggg aaccagatgg caaagaagtc ggtgaagaag aagaaggcag
      661 tcaagaagaa ggcgaagctg gcgccaaagc gccgggccgc caaggcggcg aaagcgcgca
      721 agcccgcgcg caaagtcgcg aagaaggcag tgaagacgtc ggcgaagaaa gcggtaaaga
      781 agacggtgcg gaagcccgcc ccgaagaaga ccaagaagcg ggtgaagaag gcggctcccg
      841 aggtcgtggc gcccttgacg gatatcggcg atctgtccgg cggcgcatcc ggtctgggcg
      901 aagtgtcctc gcccggtatt ccaaagctgt tttagacggc aagaagacgc gctcggcgcg
      961 ttatgtggtg tggaacattc cgttgcgcgc gtaacggata gacgagtgac ggtacggacg
     1021 tggcgaccaa agcgaagaag acggcaaaac ccaaggcggt caagcggccg cctgccaagg
     1081 cggcgcgcgc gaagtccgcg cctgccaaga gcgtgcgcac ggccgccaag tccaaaccgg
     1141 cgcccaagcc ggcgaaggcc gtagccgcca aaagctccgc caagactcaa cctaagaccc
     1201 aacccaaggc caaacccgag ttgaaatccg ccgctaaagt gcaagctccc gccaagagcg
     1261 cggcggccgt acgtgtgtct gtagttccgc cggttgcggc caaggtgaac ggcaagcccg
     1321 cggtgcccgt caagcccggt gtgaagcccg gcgaaaacga gtcgcctggc gatcccgaca
     1381 gccctttgct ggatatgtcc gacgtcggcg tgcgcaagat gatcgcgcgc gccaagacgc
     1441 gcggctacgt cacctatgac gaactgaaca aggtcctgcc ctccgacaag acgtcttccg
     1501 aacagatcga agacaccatg tcgatgctgt ccgagatggg catcaacgtc atcgagagcg
     1561 aggagcagga agagggcgcc gacgccgaag tgccggcgct ggccgaaggc aaggccgtcg
     1621 ccaccaccaa ggaaggcgcc gccgaacaat atgaccgcac cgacgatcct gtgcgcatgt
     1681 atctgcgcga gatgggctcg gtggagctct tgtcgcgcga aggcgaaatc gccatcgcca
     1741 agcgcatcga ggccggccgc gagctgatga tcggcgcctt gtgcgaaagc ccgcttacct
     1801 tcgaggcgct caccgtctgg cgcgacgaac tcaacgaagg caaggtgctg ctgcgcgaca
     1861 tcatcgacct cgaagccatg tatggcggcg ggcccgacgc tcaggccgct gcggccggcg
     1921 tcgctggcgg gccgccggtt ctgccgctgg tgcagatgaa cggcaatgcg gccgccccgc
     1981 cggtcgccga tttcaggcgg cccgaaccgc ccaagcccgc ggccgccccg ccgccggccg
     2041 cgcccaagcc ggaaggcgcc gaaggcgagg aactcgatcc cgccgaggcg caggccaaag
     2101 ccgatgccga agccgcggaa gcagacgatt tcgacgacga gggcaatctg ccgctctcgg
     2161 cgatggaagc cgcgctcaag ccgcaggtgc tggagaccct cgacaacatc gccgcgctgt
     2221 acaagaagct cggccgcttg caggacgctt cggtggaggc ggcgctcgcc tccgacgagc
     2281 tttccagcgg ccaggaacgc cgcttcaaga agctgcgcca cgagaccgtc gatctggtca
     2341 agggcctcaa gctcaacaac aaccgcatcg aagccctggt cgatcagatg tacggcatca
     2401 accgccggct ggtcagcctg gaaggcaagc tgctgcgcct ggccgaggcg catggcgtgg
     2461 cgcgcgagga tttcctcaag gagcatttcg gcaacgagct cgatcccaat tggacgcggc
     2521 gcgtcggccg gctcagccgc atgggctgga aggacttcat cgccgaagac cgcgacaagg
     2581 ttcatccgct gcgcgacgag atccagacct tagcgcagga gatgcgccag tcgatcgccg
     2641 atttccgccg catcgtgcag acggtgcaga agggcgagcg cgagtccggc gtcgccaaga
     2701 aggaaatgat cgaagcgaac cttcgcctcg tcatctccat cgccaagaaa tacaccaacc
     2761 gcggcttgca gttcctcgac ctgatccagg aaggcaatat cgggctgatg aaggcggtcg
     2821 ataagttcga ataccgccgc ggctacaaat tctccaccta tgccacctgg tggattcgcc
     2881 aggcgatcac gcgcagcatc gccgatcagg cgcgcaccat ccgtattccc gtccacatga
     2941 tcgagaccat caacaagctg gtgcgtacca gccgccagat gctgcacgag atcggccgcg
     3001 agccgacgcc ggaggaactg gccgaacgcc tcgccatgcc gctcgaaaaa gtgcgcaagg
     3061 tgctcaagat cgccaaggag ccgatcagcc tcgaaacgcc catcggcgac gaggaggatt
     3121 cgcatctggg cgatttcatc gaggacaaga acgcgatcct gccgatcgac gcggcgatcc
     3181 aggcaaattt gcgcgagacc accacgcggg tgctcgccac gctgaccccg cgcgaagagc
     3241 gcgtgctgcg catgcgcttc ggcatcggca tgaacaccga tcacacgctg gaagaagtcg
     3301 gccagcaatt ctcggtgacg cgcgaacgca tccgccagat cgaagccaag gcgctgcgca
     3361 aactgaaaca cccctcgcgc agccgcaaac tgcgctcatt tttggataac tgatctcaat
     3421 ggcattcaag gaaaagactc tcgcggatcg gcagagcgac ggcgcggccg cgcgtgccgc
     3481 ccaaatcgaa agggcgcggg ccgcggccct
//
LOCUS       DASWPU010000626         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_10973,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000626 DASWPU010000000
VERSION     DASWPU010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10973"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VGC27_13365"
     CDS             complement(<1..291)
                     /locus_tag="VGC27_13365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0283597.1"
                     /translation="MKEPAVRAPEDAQSDDSLFQTLIATAVDGIMVIDERGTVLIYND
                     ACERLFQYRAEEVVGQNVKMLMPAPHRDEHDSYLDRYKRTGQAHIIGVGREVQ"
     gene            651..821
                     /locus_tag="VGC27_13370"
     CDS             651..821
                     /locus_tag="VGC27_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283598.1"
                     /translation="MTQLVRRPLLWREIAILLGAKLLALTCLYFLFFGPSHRPLADAA
                     AVSARILGLPHG"
     gene            831..2387
                     /locus_tag="VGC27_13375"
     CDS             831..2387
                     /locus_tag="VGC27_13375"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY0283599.1"
                     /translation="MDIVELSRLQFAVTALYHFLFVPLTLGLAILLGIMETVYVMTNR
                     PIWRDITKFWGALFGINFAMGVATGITMEFQFGTNWAYYSHYVGDVFGAPLAIEGLMA
                     FFLEATFIGLFFFAWDKLSKIAHLIVTWCLAIATNLSALWILIANGWMQFPAGSRFNA
                     DTMRMEVSNFMEVLFNPVAQAKFVHTVSAGYVVGAVFVLAISAYYLLRRRHVELAKRS
                     MTVAVSFGLASALSVVVLGDESGYTAGLNQKMKIAAIEAMWNTEPAPASFTVFGIPDL
                     KARTTHYEVKVPWVLGLIATRSADTPVPGINDLVALAEARIRNGIVAYDALAKLKADR
                     GNAELRRTLDAHVEDLGYALLLKRFRPDVQNATEAEIVAAANSTIPDVPVLFWSFRFM
                     VGLGFYFIALFAFAFWLSAKRKLDAYPLFLKVAFWSLPLPWIAAELGWIVAEYGRQPW
                     VIEGVLPTALGVSSTTAGNVLFSLLGFVVFYSSLAVADVYLMVKYIRLGPDGTLGHAV
                     ASPSTREPAE"
     gene            2393..>2748
                     /gene="cydB"
                     /locus_tag="VGC27_13380"
     CDS             2393..>2748
                     /gene="cydB"
                     /locus_tag="VGC27_13380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY0283600.1"
                     /translation="MDYETLRLIWWALLGILLIGFAIMDGFDLGSAILLPFVGRTDLE
                     RRVVINTVGPVWEGNQVWLILGAGAIFAAWPAIYAVAFSGFYLAMLLLLCSLILRPVG
                     FKFRSKMPGERWRTIW"
BASE COUNT          436 a          864 c          860 g          588 t
ORIGIN      
        1 ctgaacctcc cgcccgacgc cgatgatatg ggcctggccg gtgcgcttgt agcggtcgag
       61 ataggaatcg tgttcgtcgc ggtgaggcgc cggcatcagc atcttgacgt tctgtcccac
      121 cacctcctcg gcgcgatact ggaacagccg ttcgcaggcg tcgttataga tgagcacggt
      181 tccgcgttcg tcgatcacca tgatgccgtc cacggcggtg gcgatcagcg tctgaaacag
      241 cgagtcgtcg ctttgcgcgt cttcaggcgc ccgtacggca ggttccttca tgtgcctttg
      301 cgagttgaac ttagtccacg tgtaacagga cggccggtcc gcgccgacac gcacggcgca
      361 aaaccgggaa aatgaccggc cggacatcgg ccggcagcgt caaaacgtcc cctggcactt
      421 ccctggcacc cttcattcga ataattcaaa tttgtaccgc agaattgatt tccgtcaaat
      481 acgagattcg gggcgtcatg acaaaagcga gacttgaccg gggaaaggga gcgtctcatg
      541 ccggggggtg gagctggagt cgtgggggtg gggattctcg cattaaccgc tttttccgcc
      601 gtgccggccg tcgtaccctc gaagcgaagt cgggcaaaga agaacggccg atgactcagc
      661 ttgttcgcag gccgcttttg tggcgcgaga tcgcgattct gcttggcgcc aaactgctgg
      721 cgctgacctg cctttatttc ctgtttttcg gcccgtcgca tcgcccgctg gccgatgccg
      781 ctgcggtctc cgcccggatt ctgggcctcc ctcatggcta ggtaaccatc atggatatcg
      841 tcgaactttc tcggctgcaa ttcgccgtca ccgcgctcta ccatttcctg ttcgtgccgc
      901 tgacgctcgg cctcgccatc ctgctcggca tcatggaaac cgtgtacgtg atgacgaacc
      961 gcccgatctg gcgcgacatc accaaattct ggggcgcgct gttcggcatc aatttcgcga
     1021 tgggggtggc gaccggcatc accatggaat tccagttcgg caccaactgg gcctattact
     1081 cgcattacgt cggcgacgtg ttcggcgcgc cgctggccat cgaaggattg atggccttct
     1141 tcctggaagc gacgttcatc ggcctgtttt ttttcgcctg ggacaagctg tcgaagatcg
     1201 cccacctgat cgtgacctgg tgccttgcca tcgccaccaa cctgtcggcg ctgtggatcc
     1261 tgatcgccaa cggctggatg cagttccccg ccggctcgcg cttcaacgcc gacaccatgc
     1321 gcatggaagt ctcgaatttc atggaggtgc tgttcaaccc ggtggcgcag gccaagttcg
     1381 tgcacacggt cagcgccggc tacgtcgtcg gtgcggtctt cgtgctggcg atcagcgcct
     1441 attatctgct gcgccgccgc catgtggaac tggccaagcg ctcgatgacg gtggcggtaa
     1501 gcttcgggct ggcctccgcg ctcagcgtgg tcgtgctggg cgacgagagc ggctacaccg
     1561 ccggtttgaa ccagaagatg aagatcgccg ccatcgaggc gatgtggaac accgagcccg
     1621 cgccggcgtc gttcaccgtc ttcggcattc ccgacctcaa ggcccgcacc acccattacg
     1681 aggtgaaagt gccctgggtg ctcggcctga tcgccacacg ctcggccgat acgccggttc
     1741 cgggcatcaa cgatctggtc gcgttggccg aggcgcgcat cagaaacggc atcgtcgcct
     1801 acgacgcgct ggccaagctg aaagccgacc gcggcaacgc cgagctgcgc cggacgctcg
     1861 acgcccatgt ggaagatttg ggctatgcgc tgctgctcaa gcgcttccgt cccgacgtgc
     1921 agaacgcgac cgaggcggaa atcgtcgccg cggccaactc gacgattccc gacgtgccgg
     1981 tgctgttctg gtcgttccgt ttcatggtgg ggctgggctt ctacttcatc gcgctgttcg
     2041 ccttcgcgtt ctggctctcg gccaagcgca agctcgacgc ctatccgctg ttcctgaagg
     2101 tggcgttctg gagtctgccg ctgccctgga tcgccgccga actgggctgg atcgtcgccg
     2161 aatacggccg ccagccctgg gtgatcgaag gcgtgctgcc gaccgcgctg ggcgtctctt
     2221 cgaccaccgc cggcaacgtg ctgttcagtc tgctcggctt cgtcgtcttc tattcgagcc
     2281 tcgcggtcgc tgacgtatac ctgatggtga aatatatccg gctcggtccg gacggcacgc
     2341 tcggccacgc cgtggcgtcg ccgtccaccc gcgaaccggc ggagtgacgg ctatggatta
     2401 cgaaacgctg cggcttatct ggtgggcgct gctcggcatt ctgctgatcg ggttcgccat
     2461 catggacggt ttcgatctcg gctccgcgat cctgctgccg ttcgtcgggc gcaccgatct
     2521 ggagcgccgg gtggtgatca atacggtcgg gccggtgtgg gaaggcaacc aggtctggct
     2581 gatcctcggc gccggcgcga tcttcgcagc gtggccggca atctatgcag tggcgttctc
     2641 gggtttctac ctggcgatgc tgttgctgct gtgttcgctg atcctgcggc ccgtgggctt
     2701 caagttccgc agcaagatgc cgggcgagcg ttggcggacg atttggga
//
LOCUS       DASWPU010000627         7060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_73001,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000627 DASWPU010000000
VERSION     DASWPU010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73001"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1071)
                     /locus_tag="VGC27_13385"
     CDS             complement(142..1071)
                     /locus_tag="VGC27_13385"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY0283601.1"
                     /translation="MTAWTKDEGARGWLAYGALLLAGGVLYVVCREFPADLPFWMPWE
                     FSWPAYLATALSLAWFFRGLSRLPASERPAVWRSAAFVLGVVADYAVLQTHVDYLAQH
                     MFFVHRWAHFVLHHAGAFLIALGVAGPVVRAGMPDFLKPLLDTRPVRRAVDYMQHPAV
                     APVLFVGLLYFWLVPGLHTIVMLDRNLYDIMNWTMAINGIFFWCLILDPRPKPPARIS
                     SLMRALLILVIELPQMVLGAILSLSNTDYYPVYNICGRIFDMTALNDQHYGGLIIWLP
                     GTLTSFAAIIAVLVVLRINEEKAEKVRTSLPSP"
     gene            1132..1761
                     /locus_tag="VGC27_13390"
     CDS             1132..1761
                     /locus_tag="VGC27_13390"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0283602.1"
                     /translation="MAGLDPAMTKWDGHDIPPIMNWSRPALSLALAVTLAGCGPDKPW
                     HATDITGAMPRLEFAMTRANDGMKVSAGDYLGRVTILYFGYTNCPDICPTTLANLSDM
                     LQQLGRDADKVRVLFVTVDPNRDTPPILKKYAAAFAPQIDGLRGSDNALAGLARRYRV
                     AYGVKTRPAYEVMHSNAVFFFDATGRARLVTLNTDDTAELAEDVKRLLR"
     gene            complement(1826..2533)
                     /locus_tag="VGC27_13395"
     CDS             complement(1826..2533)
                     /locus_tag="VGC27_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0283603.1"
                     /translation="MSAPADDTISTANGPVEIHAVHHASLMLTWNGKHVLVDPAPVSE
                     AGDPAAAYRALPKPDEIVITHIHGDHFNVPIIEAVAGEDTVIVVPKEVYDALPAGLKA
                     RAEIMNNGDKDVVDAIPIEAVPAYNTTPGRTKFHVKGAGNGYVLTFGDKRVYIAGDTE
                     ETPELAHLSGIDAAFLPMNLPYTQEVGAAAKWVKNFKPGVVYPYHFRNADGTMSDLAA
                     FKALVDGASEVRVLNWY"
     gene            complement(2712..4118)
                     /gene="lpdA"
                     /locus_tag="VGC27_13400"
     CDS             complement(2712..4118)
                     /gene="lpdA"
                     /locus_tag="VGC27_13400"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY0283604.1"
                     /translation="MVDNSNSFDVIVVGGGPGGYVCAIRAAQLGLKTAVVERDKLGGI
                     CLNWGCIPTKALLRSSEIWHLMHRLNEFGFSADNLKFDLAKIVERSRKVSQQLSNGVG
                     YLMKKHKIAVIAGEAKISGRGKLAVTKDGATTEVEGKNIVLAIGARPRTLPGLEADGK
                     LVWTAREAMIPTSLPKSLLVVGSGAIGIEFASFYRTLGSEVTVVEVLDRVLPVEDEEI
                     SQLAHKAFVKQGMKIKVSTTVEKLEKGADSVTATLKTNDGKTETITADRVILAVGIVG
                     NVEKIGLENVGAKVEKTHVVVNQWGETGVPGLWAIGDLVGPPWLAHKAMHEGVIVAER
                     IAGVKGVHPLDASKVPGCTYCWPQVASVGMTEAKAKADGRKVKVGRFPFIGNGKAIAL
                     GDSEGLVKTVFDADTGELLGAHMIGAEVTELIQGYTVARSGELTDVELASTIFAHPTL
                     SEMMHEAVLDADNKALHI"
     gene            complement(4209..5522)
                     /locus_tag="VGC27_13405"
     CDS             complement(4209..5522)
                     /locus_tag="VGC27_13405"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEY0283605.1"
                     /translation="MSIQILMPALSPTMEEGKLAKWLVKEGDTVKSGDILAEIETDKA
                     TMEFEAVDEGRIGKILVPEGTEGVKVNAPIAILLAEGESAGDADIPAAMTAIKEAVTE
                     VAQPKAAAPVKPAPVAVPAAAPSGTRLFASPLARRLARQKGIDLASLAGSGPRGRIVK
                     SDIEGAKPGAAKPAAGAAVSGIAPLPDAKLFYKPDDYVEIPHDLMRKSIAKRLSSATA
                     LIPHFYLTIDCNIDALMATRAHLNEASPKPVLRPAEGGTGGDEGAYKLSVNDFIVKAA
                     ALALMHVPEANASWTDSAILRHKHADVGVAVALDFGLITPIVFHAEEKGLAVISGEVK
                     ALAERARAKKLKPQEYEGGSFAISNLGMFGIKNFTAVINPPHAGILAVGAGEQRAVVT
                     GGKLEIATVMTVTMSCDHRVIDGATGARFLQVFKQYIEEPAAMLL"
     gene            complement(5813..>7060)
                     /locus_tag="VGC27_13410"
     CDS             complement(5813..>7060)
                     /locus_tag="VGC27_13410"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF008854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEY0283606.1"
                     /translation="EGTEGVKVNSPIAELIGEGEDSSSSASPLEGEAVLGARARATET
                     VGGTAAASPLPPARAPQATPASRDLPLKGGGKDVTADPSIPAGTEFDTITVREALRDA
                     MAEEMRRDDTVFLMGEEVAQYQGAYKVSQGLLEEFGERRVIDTPITEHGFAGLGVGAA
                     FNGLKPIVEFMTWNFAMQAIDQIVNSAAKTRYMSGGQMHCPIVFRGPNGPASRVGAQH
                     SQDFSAWYAHVPGLKVISPYFAADAKALLKAAIRDPNPVIFLENEIVYGRSFPVPKLD
                     DYVLPIGRARVIKQGADVTIVAHSISVGMILEAAEVLAKEGIDAELIDLRTLRPLDTG
                     TVIESVKKTNRLVTVEQGWPVCSIGSEIASQVVETAFDWLDAPPVKIAGKDVPTPYAA
                     NLERLALPTVDDIVAAAKAVCYR"
BASE COUNT         1250 a         2271 c         2222 g         1317 t
ORIGIN      
        1 cccaccctcc ccttgaggga gggtcgaaat tttccgcgag cgatagcgag aagaaaattt
       61 cggggagggg tgatgcgcca acaatacccc tccccgaaaa attcttgagc gtacgctgcc
      121 gctcgaattt ttcgaccctc ctcaagggga gggtaaagat gtgcgcacct tttccgcttt
      181 ctcctcgttg atcctgagca cgaccagcac ggcgatgatc gcggcgaagc tggtcagcgt
      241 tccggggagc cagatgatca gcccgccgta atgctggtcg ttcagcgccg tcatgtcgaa
      301 gatgcggccg cagatgttgt agacggggta atagtcggtg ttcgacagcg acaggatcgc
      361 gcccagcacc atttgcggca gctcgatgac caggatgagc agcgcgcgca tcaggctgga
      421 aatccgcgcc ggcggcttgg ggcgcggatc gaggatcagg caccagaaga agatgccgtt
      481 gatcgccatg gtccaattca tgatgtcgta gagattgcgg tcgagcatga cgatggtgtg
      541 caggcccggg accagccaga aataaagcag ccccacgaac agaaccggcg cgacggcggg
      601 atgctgcatg tagtccaccg cgcgccgtac cggccgggtg tcgagcagcg gctttaagaa
      661 gtccggcatg ccggcgcgca ccaccggtcc cgcaacaccc agcgcgatta ggaaggcgcc
      721 cgcatgatgc agcacgaaat gggcccagcg atggacgaag aacatatgct gcgcgagata
      781 gtccacatgg gtttgcagca cggcgtaatc ggccactacg cccagcacga acgccgcgct
      841 gcgccacacg gccgggcgct cggaggcggg caagcgcgac aggccgcgga agaaccaggc
      901 aagcgaaagc gccgtcgcca gataggcggg ccaggaaaat tcccacggca tccagaatgg
      961 caggtcggcg gggaactcgc ggcagacgac gtaaagcacg ccgcccgcga gcagcagcgc
     1021 gccataggcg agccagccgc gcgcgccctc gtcttttgtc caggcagtca tcaccgtcat
     1081 tttgccgatt tacacggccc gcgccaggag gtcccaaccc acgcattcgt catggccggg
     1141 ctcgacccgg ccatgacgaa atgggatggg catgatattc caccgattat gaattggagc
     1201 cgtcctgcac tttctttggc gctagccgtc accctcgccg gctgcggccc ggataaaccc
     1261 tggcacgcga ccgacatcac cggcgccatg ccccggctcg aattcgccat gacccgcgcg
     1321 aacgacggca tgaaggtatc cgccggtgat tacctcggcc gcgtcaccat cctctatttc
     1381 ggctacacca actgtccgga catctgcccc accacgctgg ccaacctgtc cgacatgctg
     1441 caacagttgg ggcgcgacgc cgacaaggtg cgcgtgctgt tcgtcaccgt cgatcccaac
     1501 cgcgacacgc cgccgatcct gaagaaatac gcggcggcgt tcgcgccgca gatcgacggg
     1561 ctgcgcggca gcgacaacgc gcttgccggt ctggcgcgcc gctatcgcgt cgcctacggc
     1621 gtgaaaaccc ggcccgccta tgaggtgatg cattccaacg ccgtgttctt cttcgacgcc
     1681 accggccgcg cccggctggt gacgcttaac accgacgaca cggcggaact ggcggaggat
     1741 gtgaaaaggc tcctcagata agtcacctcc ccataagggg ggaggtaaat atacagatat
     1801 tgcttcgagc gcttggcgcg gccgatcaat accagttcag cacgcgcacc tcgctcgcgc
     1861 cgtccaccag cgccttaaag gcggcaagat cgctcatcgt cccatcggcg ttgcggaagt
     1921 gatagggata gacgacgccc ggcttgaaat tcttcaccca cttcgccgcc gcgcccacct
     1981 cttgggtgta aggcagattc atcggcagga acgccgcgtc gatgccggac aggtgagcca
     2041 gctccggcgt ttcttcggtg tcgcccgcga tgtaaacgcg cttgtcgccg aacgtcagga
     2101 catagccgtt gccggcgccc ttgacatgga acttcgtccg ccccggcgtc gtgttgtacg
     2161 ccggcaccgc ctcgatcgga atcgcatcca ccacatcttt gtcgccgtta ttcatgatct
     2221 cggccctggc cttcagaccg gcgggcagcg cgtcatagac ttccttcggc acgacgatca
     2281 ccgtgtcctc gcccgcgacc gcctcgatga tcggcacgtt gaaatgatcg ccatggatgt
     2341 gggtgatcac gatctcgtcg ggcttcggca aggcgcggta cgcggctgcc gggtccccgg
     2401 cctcggagac cggcgcggga tcgaccagca cgtgcttgcc gttccaggtc agcatcagcg
     2461 aggcatgatg gacggcgtgg atctccacgg gtccgttcgc ggttgaaatc gtgtcgtctg
     2521 cgggtgcgct catggcttcc cccatgcaaa gagtaagaac ggcaagaccg gcggcaatgc
     2581 gaaagcggtt catggcttgg ctttctcctt ctagagcgca atccggcaaa gtgtgaagcg
     2641 gtttgccgtc agattgcgcg aaaatcaagg aatctagagc ttgatccgat tcaattaaat
     2701 cggatcaagc tctagatatg caacgccttg ttgtcggcgt cgagcacggc ttcgtgcatc
     2761 atctcggaca atgtcggatg cgcgaagatc gtggacgcca gctcgacatc ggtaagctcg
     2821 ccggagcgcg cgacggtgta gccctggatc agttcggtga cttccgcgcc gatcatgtgc
     2881 gcgccgagca gctcgccggt gtcggcgtcg aacaccgttt tcaccaagcc ctcggaatcg
     2941 ccgagcgcga tcgccttgcc gttgccgata aaggggaagc ggccgacctt gaccttgcgg
     3001 ccgtccgcct tggccttcgc ttccgtcatg ccgacgctgg ccacctgcgg ccagcaatag
     3061 gtgcagcccg gaaccttgga ggcgtcgagc ggatgaacgc ccttcacgcc ggcgatgcgc
     3121 tccgccacga tcacgccttc atgcatcgcc ttgtgcgcca gccagggcgg gccgaccaga
     3181 tcgccgatgg cccacaggcc cggcacgccc gtctcgcccc attggttgac caccacatgg
     3241 gtcttctcga ccttcgcgcc cacgttttcg agaccgattt tttccacatt gccgacgatg
     3301 ccgacggcga ggatcacgcg atccgcggtg atggtttccg tcttgccgtc gttggtcttc
     3361 agcgtggcgg taacgctgtc ggcgcccttc tccagcttct cgacggtggt ggaaaccttg
     3421 atcttcatgc cctgtttgac gaaggccttg tgcgcaagct gcgagatttc ttcgtcttcc
     3481 accggcagca cgcggtcgag cacttccacc accgtgacct cggacccgag ggtacgatag
     3541 aagctggcga actcgatgcc gatggcgccg gagccgacca ccagcagcga tttggggagc
     3601 gatgtcggga tcatcgcctc gcgagcggtc cagaccagct tgccgtccgc ctccaggccc
     3661 ggaagcgtgc gggggcgcgc gccgatggcg agcacgatgt tcttgccctc gacttcggtt
     3721 gtagctccgt ccttggtcac agccagcttg cccctgcccg aaatcttcgc ctcgccggcg
     3781 atgacggcaa ttttgtgctt cttcatcaga taaccgacgc cgttcgacaa ttgctgcgac
     3841 accttgcgcg agcgttcgac gatcttcgcc agatcgaatt tgagattgtc ggccgagaat
     3901 ccgaactcgt tcagccggtg catcaggtgc cagatttccg aggagcgcag cagcgccttg
     3961 gtcgggatgc agccccaatt gaggcagatg ccgccgagct tgtcgcgctc gaccacggcg
     4021 gttttcaggc cgagctgcgc ggcgcggatg gcgcacacat agccgccggg cccgccgccc
     4081 accacgatga cgtcgaaact attggaattg tcaaccatcg gtctattgcc tcgatctttc
     4141 gacaagctct gaacaccaca tgagggcctt catccgagct tgtcgcagga tgaaggcaga
     4201 cgcatcgatt atagcagcat tgcggccggc tcttcgatgt attgcttgaa gacctggagg
     4261 aagcgcgcgc ccgtcgctcc gtcgatgacg cggtggtcgc aggacatggt gacggtcatc
     4321 accgtggcga tttcgagctt gccgcctgtc accacggcgc gctgttcgcc cgctccgacg
     4381 gcaagaatgc ccgcatgcgg cggattgatg acggcggtga agtttttgat cccgaacatg
     4441 ccgagattgg agatggcgaa gctgccgcct tcatattcct gcggcttgag cttcttggcg
     4501 cgggcgcgct cggccagcgc tttcacttcg ccggaaatga cggccaggcc cttttcctcg
     4561 gcatggaaca cgatcggcgt gatgagcccg aaatcgagcg ccaccgccac gccgacatcg
     4621 gcgtgcttgt ggcgcaggat ggcgctgtcg gtccagctcg cattggcttc cggcacgtgc
     4681 atgagcgcta gcgcggcggc cttgacgatg aaatcgttga ccgagagctt gtaggcgcct
     4741 tcgtctcccc cggtcccgcc ttcggcgggc cggaggacag gttttggcga cgcttcgttg
     4801 aggtgcgcac gcgtagccat cagcgcatcg atgttgcagt cgatggtcag atagaaatgc
     4861 gggatcaagg cggtcgcgga agaaagccgc ttggcgatcg acttgcgcat cagatcgtgc
     4921 gggatttcga catagtcgtc cggcttgtag aacagtttgg cgtcgggcag cggcgcgatg
     4981 ccggagacgg ccgcgccggc ggcaggcttc gcggcgccgg gcttggcgcc ctcgatgtcg
     5041 gacttcacga tgcgcccgcg cgggccggag ccggccaggc tcgcgagatc gatgcctttc
     5101 tgccgggcga ggcggcgcgc cagcggcgaa gcgaacaggc gcgtgccgga tggcgcggcg
     5161 gcgggcaccg ccaccggtgc gggcttaacc ggagcggctg cttttggctg ggcgacttcg
     5221 gtcactgcct ctttgatcgc cgtcatcgcc gccggaatat cggcgtctcc ggcgctctcg
     5281 ccttccgcga gcaggatcgc gatgggcgcg ttgaccttca cgccctcggt gccttcggga
     5341 accaggattt taccgatgcg gccttcgtcc accgcttcga attccatcgt cgccttgtcg
     5401 gtttctatct cggccaggat gtcgccggat ttcaccgtat cgccttcctt gaccagccac
     5461 ttggcgagct tgccctcttc catggtgggc gacagggcgg gcatgaggat ttggatcgac
     5521 atcagttcgc ctcaagcggc cccagttcgg ccgcatgttg ttcccagtgc gtgccgtcga
     5581 acggcgccgc gccgcagtgg cagccgccct ttttccaggt catgctggag gactcagcca
     5641 tcggcccctc attcattcgt catggccgcc accccggtcc gcgcgaagcg cggccggagt
     5701 gcaagcgctt gaggcggcca tccagtagct gcgcggcgga gcggctcttc gctggatggc
     5761 cgggtcaagc ccggccatga cgaaaaagga agatacgacc gccaattgtc gctcatcgat
     5821 aacacaccgc tttcgcagcc gcgacgatat cgtccaccgt cggcagcgcc agcctttcca
     5881 gattggcggc atagggcgtc ggcacgtcct tgccggcgat tttcacgggc ggcgcgtcga
     5941 gccagtcgaa cgccgtctcc accacctgcg aagcgatttc cgagccgatg gagcagaccg
     6001 gccagccctg ctcgaccgtg acgaggcggt tggtcttctt cacggattcg atcaccgtgc
     6061 cggtgtcgag cggccgcagc gtgcgcaggt cgatcagctc ggcgtcgatg ccttccttgg
     6121 ccagcacttc ggcggcctcg aggatcatcc cgaccgagat cgaatgcgcc acgatggtca
     6181 cgtccgcgcc ctgcttgatc actcgcgccc tgccgatggg cagcacgtaa tcgtcgagct
     6241 tgggcaccgg gaaggagcgg ccatagacga tttcgttttc caggaagata accggattgg
     6301 ggtcgcggat cgcggccttc aacagcgcct tggcgtcggc ggcgaaatag ggcgagatca
     6361 ctttcaggcc cggcacatgc gcgtaccagg cggaaaaatc ctggctatgc tgcgcgccga
     6421 cgcgcgacgc cggcccgttc ggcccacgga agacgatggg gcagtgcatc tgaccgcccg
     6481 acatgtagcg cgtcttggcg gcggaattga cgatctggtc gatcgcctgc atggcgaagt
     6541 tccaggtcat gaactcgacg atgggtttca gcccgttgaa cgccgcgccg acgccaaggc
     6601 ccgcgaagcc gtgctcggtg atcggcgtgt cgatgacgcg acgctcgccg aattcctcca
     6661 gcagcccctg gctgactttg taggcgccct ggtactgcgc gacttcctcg cccatcagga
     6721 acacggtgtc gtcgcggcgc atttcttcgg ccatggcgtc gcgcaaggcc tcgcgcaccg
     6781 tgatggtgtc gaattcggta ccggcgggga tcgaagggtc ggcagtaacg tctttacctc
     6841 cccccttgag ggggaggtcg cgagacgcag gcgtagcctg cggcgcgcgg gcgggaggta
     6901 atggggatgc tgccgcagta ccccccaccg tttcggtcgc gcgtgcccgc gcgcccaaaa
     6961 ccgcctcccc ctcaagaggg gaggctgaac ttgaactgtc ctcgccctcg ccgatcagtt
     7021 cggcgatggg agaattcacc ttgacgcctt cggtgccttc
//
LOCUS       DASWPU010000628         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_48419,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000628 DASWPU010000000
VERSION     DASWPU010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48419"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /gene="nuoG"
                     /locus_tag="VGC27_13415"
     CDS             <1..1747
                     /gene="nuoG"
                     /locus_tag="VGC27_13415"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY0283607.1"
                     /translation="ADFHRFDENKRGVEDKYMGPLIKTVMTRCIHCTRCIRFATEVAG
                     VPELGATGRGEDMEVTTYLEKAFASELSGNVVDLCPVGALTSKPYAFNARPWELKKTE
                     SIDVMDAQGCNIRVDTRGREVLRVLPRLNEEINEEWISDKARHACDGLSRQRLDTPYV
                     RKNGKLAPATWAEAFAAIAARVEESKPERMEAIVGDLAAAEETKALKDLMAALGVVHL
                     DCRQDGAKLSGPRPAYLFNTGIAGVEAADALLLIGTNPRWEAPVLNARIRKTWLTGNL
                     KIANLGTPVDLTYPAEQLGASAKVLEALADGSHPFAAVLKNAKRPMLIVGASAVARSD
                     GAAILRLAAKIAMETGMIGPAAPLSEGGWNGFNVLHAAASRVAALDLGFLPGKGGRDV
                     AGIVDGAEKGEIDFLYSLGADELPAGKLGQAFVVYQGSHGDAGAHRADVILPGAAYTE
                     KDGLYVNFEGRVQEAERAAFPPGEAKEDWAILRALSEALGMPLPYDNREALRVAIIVA
                     APHFAVLGKTPVHDAADPALWSAVGAAGPLDPALPLGSAIADYYLTNPIARASRTMAE
                     CSRVLLHGAPSMAAE"
     gene            1751..>2497
                     /locus_tag="VGC27_13420"
     CDS             1751..>2497
                     /locus_tag="VGC27_13420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY0283608.1"
                     /translation="MIFVPYLQHLGLPYAWAWTIGQVVIIGVFQVVLLVSVAFWILAD
                     RKIWAAVQMRRGPNVVGVFGLLQTFADAFKFMFKESIIPAGANKVIFLLAPLITTILA
                     FDGWAVVPFDSGWVVADINVGILYLFAVSSLGVYGVIMGGWASNSKYPFLAALRSAAQ
                     MVSYEVSIGFVLITVLLFAGSLNLSAIVRAQEHGWFAFTILFPMFVIFFISALAETQR
                     PPFDLLEAESELVAGFMVEYSSTPFLLYFLG"
BASE COUNT          396 a          813 c          823 g          465 t
ORIGIN      
        1 cgccgacttc caccgcttcg acgagaacaa gcgcggcgtc gaagacaaat atatgggccc
       61 gctgatcaag acggtgatga cccgttgcat ccactgcacg cgctgcatcc gcttcgccac
      121 ggaagtggcg ggcgtgccgg aactgggcgc caccggccgc ggcgaggaca tggaggtcac
      181 gacctatctg gaaaaggcat tcgcctccga gctttccggc aatgttgtgg acctttgccc
      241 ggtgggtgcg ctgacctcca agccttatgc cttcaacgcg cggccgtggg aattgaagaa
      301 gacggaatcc atcgacgtga tggacgccca aggctgcaac atccgcgtcg atacgcgcgg
      361 tcgcgaagtg ctgcgggtgc tgccgcgcct caacgaagag atcaacgagg aatggatttc
      421 cgacaaggcg cgccatgcct gcgacgggct ttcgcgccag cgcctcgaca cgccctatgt
      481 ccgcaagaac ggcaagctgg cgcccgcgac ctgggcggaa gcctttgccg ccatcgccgc
      541 gcgcgtggag gagagcaagc ccgaacgcat ggaggccatc gtcggcgatc tggcggcggc
      601 ggaggagacc aaggcgctga aggatttgat ggcggcgctc ggcgtcgtcc atctcgactg
      661 ccgccaggat ggcgccaagc tctccggccc gcgtccggcg tatttgttca acaccggtat
      721 cgcgggcgtg gaagccgccg atgcgctgct cttgatcggt accaatccgc gctgggaagc
      781 gccggtgctg aacgcgcgca tccgcaagac ctggctcacc ggcaatctca agatcgccaa
      841 tctcggaacg ccggtcgatc tcacctatcc ggccgagcag ctcggcgcga gcgcgaaggt
      901 gctggaagcg ctggccgacg gcagccatcc gtttgccgcc gttctgaaga atgccaagcg
      961 gccgatgctg atcgtcggcg ccagcgcggt ggcgcgctcc gatggtgcgg cgatcctgcg
     1021 gctggcggcg aagatcgcca tggagaccgg catgatcggc cccgccgcac ctttgtctga
     1081 aggcgggtgg aacggattca atgtgctgca cgcggccgcc tcgcgcgtgg cggcgctcga
     1141 cctcggcttc ctgccgggca aaggcgggcg cgacgtggcg ggcatcgtgg acggcgccga
     1201 gaagggcgag atcgacttcc tctattcgct cggcgcggac gaattgcccg ccggcaaact
     1261 cggccaagcc ttcgtcgttt atcaaggcag ccacggcgat gcgggcgcgc accgggccga
     1321 cgtgatcctg cccggcgcgg cttacaccga gaaggacggg ctttacgtca atttcgaagg
     1381 ccgggtgcag gaggccgaac gcgccgcttt cccgccgggc gaggcgaaag aggattgggc
     1441 gatcctgcgc gcgctatccg aggcgctggg catgccgctg ccttacgaca atcgcgaagc
     1501 gttgcgtgtg gcgattatcg tggccgcgcc gcatttcgcc gtcctgggca aaacgcccgt
     1561 gcatgacgcc gccgatccag cgctctggag cgcggtgggc gcggcaggtc cgctggaccc
     1621 ggcactgccg ctcggctcgg cgattgcgga ctattacctc accaatccca tcgcgcgggc
     1681 cagccgcacg atggccgaat gctcgcgcgt gctcctgcac ggcgcgcctt cgatggcggc
     1741 ggagtaggcg atgatcttcg ttccctacct ccagcacctc ggactgcctt acgcctgggc
     1801 gtggacgatc ggccaggtcg tcatcatcgg ggttttccag gtggtgctgc tggtgtcggt
     1861 cgccttctgg attctcgccg accgcaagat ctgggcggcg gtgcagatgc gccgcggccc
     1921 caacgtggtg ggcgttttcg gtttgctgca aaccttcgcc gacgctttca agttcatgtt
     1981 caaggaatcg atcatcccgg ccggggccaa caaggtgatc ttcctgctgg ccccgctgat
     2041 caccaccatc ctggccttcg acggctgggc ggtggtgccg ttcgacagcg gctgggtggt
     2101 ggccgacatc aatgtcggca tcctctatct cttcgcggtg tcctcgctcg gcgtctatgg
     2161 cgtcatcatg ggcggctggg cgtcgaattc gaaatatccg ttcctcgcgg cgctgcgttc
     2221 cgcggcgcag atggtgtcct acgaagtttc catcggcttc gtgttgatca cggtgctgct
     2281 gttcgccggg tcgctgaacc tctcggccat cgtgcgcgcc caggagcatg gctggttcgc
     2341 cttcaccatc ctgttcccga tgttcgtgat ctttttcatt tccgcgctcg ccgaaaccca
     2401 gcgcccgccg ttcgacctgc tggaagcgga atccgaactc gtcgccggct tcatggtgga
     2461 atattcctcg acgccgttcc tgctctactt cctcggc
//
LOCUS       DASWPU010000629         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_18140,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000629 DASWPU010000000
VERSION     DASWPU010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18140"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..1304)
                     /gene="argC"
                     /locus_tag="VGC27_13425"
     CDS             complement(354..1304)
                     /gene="argC"
                     /locus_tag="VGC27_13425"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY0283609.1"
                     /translation="MKARIFIDGGAGTTGLEIRERLAGRSDLKLLQLAEAERKDPRAR
                     AKALNEADLVILCLPDEAAREAVRLIENPAVRVIDASTAHRTAPGWVYGFPEMEPDQR
                     EKIAAAKRVSNPGCYPTGFLALVRPLTRAGIIPADFPLTVNAVSGYSGGGKAMIAEFR
                     DKASPAYVETVVRTYGLALSHKHAPEMQVHAKLAHQPVFAPSVGRFYRGMIVEVPLQL
                     WALPGKPLAAELHAVLEGAYRGQALVEVESLEGTMQLKSLDAEMLKLTNRLKLFVFGN
                     DKTGQARLAAVLDNLGKGASGAAVQNLNLMLGLPETAGLV"
     gene            complement(1373..1741)
                     /gene="rpsI"
                     /locus_tag="VGC27_13430"
                     /pseudo
     CDS             complement(1373..1741)
                     /gene="rpsI"
                     /locus_tag="VGC27_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
     gene            complement(1950..2378)
                     /gene="rplM"
                     /locus_tag="VGC27_13435"
                     /pseudo
     CDS             complement(1950..2378)
                     /gene="rplM"
                     /locus_tag="VGC27_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
     gene            2661..>2978
                     /locus_tag="VGC27_13440"
     CDS             2661..>2978
                     /locus_tag="VGC27_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY0283610.1"
                     /translation="MLTYPDDLIKSILRSVKTIAMIGASGNEMRPSYFAMKYLLAKGY
                     VIHPVNLGLAGKEILGQKVYASLKEVPAPVDMVDIFRGSDAAPAIVAEALAEKDRLGV
                     KVIW"
BASE COUNT          546 a          962 c          876 g          594 t
ORIGIN      
        1 gcttgggctg aggaatcatc tccaaaccac gtcgtcggat cgacccaatc gggcacactc
       61 ggcaagctgg aacaggcact tgcgctgatc gcgaacatga acactacggc aacacgcaag
      121 atgtctttcc cagtggcgtg aaagcctagc atcatggaac actccttagc cgcggttgcg
      181 ctgcgccagc ctgcctacac catatacaag gccctccgac gcgatccaag catttcaagc
      241 cacgcaacta catgaaatct tgtgacaaac ttgcacaaag acacagcctt acagacagcg
      301 ccaataggat aggcgccgcg ctgcagactt acaggaagat caaggcgtaa acctcagacc
      361 agccctgcgg tttcgggcaa gcccagcatc agattgaggt tctggacggc cgccccgctt
      421 gcgcccttgc cgagattgtc gagcacagct gcgaggcgcg cctggccggt cttgtcgttg
      481 ccgaagacaa acagcttcaa acggttcgtc agcttcagca tctccgcgtc caagcttttg
      541 agctgcatcg tgccttcgag gctttcgacc tcgaccagcg cctgtccgcg ataggcccct
      601 tcgagcacgg catgaagttc cgccgccaac ggcttgcccg gcaaagccca tagctgcaac
      661 ggcacttcca caatcatgcc gcgatagaag cgccccacgc tcggtgcgaa gaccggttgg
      721 tgggccagct tggcatgcac ctgcatttcc ggcgcatgct tgtgggagag tgctagcccg
      781 taggtgcgca ccaccgtttc gacataggcg ggcgatgcct tgtcccggaa ctccgcgatc
      841 atcgccttgc cgccgcccga atagccggag accgcgttca cggtgagggg aaaatccgcc
      901 ggaatgattc cggcgcgcgt cagcggccgc accagcgcca ggaagccggt cggatagcag
      961 cccggattgc tcacccgctt ggcggcggcg atcttctcgc gctgatcggg ttccatttcg
     1021 ggaaagccgt aaacccaacc gggcgccgtg cgatgcgctg tggaggcgtc gatcacccgc
     1081 acggccggat tttcgatcag cctcaccgcc tcgcgcgccg cctcgtccgg caggcaaagg
     1141 atgacgaggt ccgcctcgtt caaagccttc gcgcgcgcgc gcggatcctt gcgttccgcc
     1201 tcggcgagct gcagtagctt aagatcgctg cggccggcca gccgctcgcg aatttccagg
     1261 ccggtcgtac cggccccccc atcgatgaaa attcttgcct tcatggctgc cacctataac
     1321 aaaaagggcg ccccggcggg acgccctttt tcgacttaag atcgaaagac cgtcaccgct
     1381 tcgagaactg gaagctgcgg cgggcctttg gcctgccgta cttcttgcgt tcgactgcgc
     1441 gcggatcgcg ggtgaggaag ccgcccggct tgagcaccgc gcgcaactcc ggttcgtagt
     1501 tcaccagggc gcggctgatg ccgtggcgca ccgcgcccgc ctgtccggaa agcccgccgc
     1561 ccgtgaccgt ggcgatcaca tcgaactgtc cgtcgcgggc cgccgcgatc agcggctggc
     1621 gcaggatcat gcgcagcacg ggacgtgcaa aatagatctt ctcttccctg ttgttgacca
     1681 tgatgcggcc gctgcccggc ttgatccaga cccgcgccac cgcgttcttg cgccggccgg
     1741 tggcataagc cctgcccttg gagtcgcgct tggaggcgct gggttccgcg gcctgcgccg
     1801 cactcgtctg cggcttcgcc ttgatcagcg tcgttttcag atcgccgaag ctttttacct
     1861 cttccgccat atcaagccgc cttcggatcg ttttcgctgc gcgcaacgcg cgcggggcga
     1921 tagttggtgt tcttggggtt catcttcgcg acgtcgagcg cttctggctg ctgcgcctcg
     1981 tgatgatgct ccgcgcccgc atagacccgc agattggcga gctggcgctt gccgagcggt
     2041 ccgtgcggca gcatgcgttc caccgccttt tcgacgatcc gctcgggaaa tttgccgcgc
     2101 aggatctggc tggccgtgcg ttccttgatg ccgccgatgt agcccgtgtg gtggtaatag
     2161 actttcttct tcagcttatc gcctgtaaga agcgccttcg ccgcgttgac gatgatgaca
     2221 ttgtcgccgc agtccatatg gggcgtatag ccgggcttgt gtttgccgcg caggcgcgtc
     2281 gcgacgatcg cagcgaggcg gccgacgacg agcccttcgg catcgatcac cacccacttc
     2341 ttctcaatct cggaggcctt ggccgagtag gttttcatta taacttctcc atggccgggc
     2401 agcggagcgt atgcgtagcg cgacccggcc accattgaac cggcgcggca tatagggggt
     2461 acggcgatga gcgtcaagca gaatgcctga ttttaccgtt gttttatgta tacggtaaca
     2521 taataccgtt gtaatgcggc accttgtttt gggctaaccc gtcatggccg cgaatgcggg
     2581 ccacaaaaca tttcaaccca accaagcgcc ggctctccgg ccgcagttgc aggccgtgac
     2641 agttacggct taattgagca atgctcactt accccgacga cctcatcaag agcattctgc
     2701 gctcggtcaa gaccatcgcc atgatcggcg ccagcggcaa cgaaatgcgc cccagctact
     2761 tcgcgatgaa atatctgctg gccaaaggct acgttattca tccggtcaat ctgggcttgg
     2821 cgggcaagga aatcctgggg cagaaggttt atgcctcgct gaaagaggtg cccgcccctg
     2881 tcgatatggt cgatatcttc cgtggcagcg acgccgcgcc cgccatcgtc gccgaagctc
     2941 tggccgaaaa ggaccgcctc ggcgtcaagg tgatctgg
//
LOCUS       DASWPU010000630         5308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_54300,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000630 DASWPU010000000
VERSION     DASWPU010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54300"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VGC27_13445"
     CDS             complement(<1..213)
                     /locus_tag="VGC27_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283611.1"
                     /translation="MSTADGRWALVSTSLTAVIAGSIAWLAIGTMAPANSRGFEAANF
                     LPYRLFQQLSQSSGNSLYAALGISGAP"
     gene            complement(558..1460)
                     /locus_tag="VGC27_13450"
     CDS             complement(558..1460)
                     /locus_tag="VGC27_13450"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEY0283612.1"
                     /translation="MNSDRPPETVRAYLEQLRRALKGAPPGLISDALADCEEHLNIEV
                     AQNPELDEPTILASVVETYGSPQEIAEEYRDMEATISTPFPKSEERENRRYGFFNVIS
                     DPRTYGALLYMLLALITGIFYFTWTIVGISLTAGFAILILGIPFALLFVGSIRMLSHV
                     EGRIVEGLLGVRMPRRLPSTMPSDESIWTRIKEALLDMRTWTSMFYLLLMLPLGGIYF
                     VIAIVGLSLSLGVSGCAIYALITNHSNFQVDGLPWLDHLLHTAPGLMLVTLLGILLFF
                     VVLHVARAVGWMHGRIAEGLLVRL"
     gene            complement(1438..1788)
                     /locus_tag="VGC27_13455"
     CDS             complement(1438..1788)
                     /locus_tag="VGC27_13455"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0283613.1"
                     /translation="MAGLSSLVLLAVLARAGREMYGYEIAKAVKAEGEGGLIFKQGAI
                     YPVLRSLNALGLLESRVEASAFGPPRRYYNITDDGRKALADWQFAWRDMREFVDDALK
                     GGTGGMAHEFRPTA"
     gene            complement(2019..2636)
                     /locus_tag="VGC27_13460"
     CDS             complement(2019..2636)
                     /locus_tag="VGC27_13460"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0283614.1"
                     /translation="MVGGKIPQPMRPHGFWGRVFCWFMDRRNARAHAFALKRLDMQKN
                     DILLEIGFGTGRLARKLAKAAHEGFVGGVDPSELMLRTAEKRTKKFRKAGRVELKLGD
                     ASALPWPDGSFDKAAALHCFHFWKDPAHDIAEVRRVLKPGGLLLLILRAHKKAGPKWL
                     PNAISRSGQEIAGTLQLLANNGFGQTRIEGKAGSSAVLTAIKAEQ"
     gene            complement(2639..4222)
                     /locus_tag="VGC27_13465"
     CDS             complement(2639..4222)
                     /locus_tag="VGC27_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0283615.1"
                     /translation="MAVATVAAASGEDVVLLDMTRTKKTIFQALVDARSRFGGTRPAI
                     IDGDERVVTYDEILRASLALGHALKKGTRKGESVGIMLPTSIGAIVSFFAVSAFGRVP
                     TMGNFTNGAAAILSALRTAKVKRVVTARRFIELAKLDALAAEISMTAELIYLEDVREN
                     LSVFDKAVAGIGQFVPGLVTSRPAHESTAVILFTSGTEGEPKGVALSHSNLLSNIEQV
                     RAHVAFYPTDVMFNPLPTFHCFGLLAGALLPLILGIKAAFHPTPLQPHEIVRRVRETG
                     ATILLTTDTFMSQYARAGEQGDLNSLRLAVCGAERVRDETRQLLRKKYAIDLIEGYGV
                     TETSPVAAANQPGANRPGTVGRLLPGMQARLEPVEGIPNAGRLFLKGPNVMLGYLRPE
                     NPGVVVPLEGGWHDTGDVVSIDSEGFISIKGRLKRFAKVGGETVSLAVVENCASSLWP
                     DHSHAAIAVPDGRKGEQIVLLTTNPEAQRVDLVGWAQNHGVAEIAVPRRLVHTDDIPV
                     LGTGKTDYVKVEKIVLAES"
     gene            complement(4406..>5308)
                     /locus_tag="VGC27_13470"
     CDS             complement(4406..>5308)
                     /locus_tag="VGC27_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0283616.1"
                     /translation="DVLLSPLPTFHCFGLMAGVLSPLFLGIKAVLHPTPLRPHEIVRR
                     VRETGATILLSTDTFMSQYARAGEQGDLNSLRIAVCGAERVRDETRQLLRKKYAIDLI
                     EGYGMTETSPFAAANQPGANRPGTVGHLMAGMQARLEPAEGIPGAGRLFLKGPNVMLG
                     YLRPENPGVVVPLEGGWHDSGDVVSIDGEGFISIKGRLKRFAKVGGETVSLAVVENCA
                     SSLWPDHSHAAIAVPDGRKGEQIVLLTTNPEAQRVDLVGWAQNHGVAEIALPRRILHT
                     DDIPVLGTGKPDYVKVEKIVLAES"
BASE COUNT         1029 a         1657 c         1641 g          981 t
ORIGIN      
        1 aggcgcgccc gagatgccta acgccgcata aagcgagttg ccgcttgact gcgacagttg
       61 ctggaacagg cggtagggca ggaaattggc ggcctcgaac ccgcgcgaat tcgcgggcgc
      121 catcgtgcct atggcaagcc aggcgatgga tccggcaata acggctgtca gggaggttga
      181 aacgagggcc cagcggccgt cggcggtcga caaggcggag gcggccttcc gggcggagaa
      241 gggccaattt gggaaaaagg aaatctgcgt cggctcggat tgaccgtcca cgatggctcc
      301 tgggttatca gttccgcggc tgtacgaaag tcttcgtccc cctgtattgc gcggacgaac
      361 cggcgatacc gtcatttagc ccgaccctat actgggccgt caaccattac tgcggcgtta
      421 agtatacggc gctccgtccc tggacacttg tgccaacctg tgaatctcag tgcaaattag
      481 caaactttgg gccaaaacgg ctgcggctca tgagcattgc gctcatgagc cgcatttctg
      541 tcgctcgaac ttcgggacta aaggcgaacg agaagccctt ccgcgattcg tccgtgcatc
      601 cagccgacgg ctctggcgac gtgcaggacc acgaagaaca aaaggatgcc gagcagcgtc
      661 accagcatca ggccaggtgc cgtgtggagc agatgatcga gccatggaag gccgtccacc
      721 tggaaattgc tgtggttggt gatcagagca tagatcgcgc agcccgagac gccgagcgag
      781 agcgacagcc cgacgatggc gatcacgaaa tagatgccgc ctagcggcag catgagcagc
      841 agatagaaca tcgacgtcca ggtccgcatg tcgagcagcg cttccttgat gcgcgtccag
      901 atggactcat cggacggcat ggtggagggc aggcggcgcg gcatgcgcac ccctagcaag
      961 ccttcgacga tgcgcccctc cacatggctg agcatgcgga tcgaaccgac gaacagcagc
     1021 gcgaagggaa tgcctagaat gaggatcgcg aatccggccg tcagcgatat tccgacaatc
     1081 gtccaggtga aatagaagat gccggtgatc aaggccagca gcatgtaaag aagcgcgccg
     1141 taggtgcgcg ggtccgaaat gacgttgaaa aagccgtaac ggcgattttc ccgctcttcg
     1201 gacttcggaa acggcgtgga aatcgtggct tccatgtcgc gatactcctc tgcgatctct
     1261 tgcggcgaac cgtaggtttc caccacggag gccagaatag tgggttcgtc caattccggg
     1321 ttctgggcga cttctatgtt cagatgctct tcacaatccg ctagcgcatc ggagatcagt
     1381 ccgggcgggg cgcctttcag ggcgcggcgc agctgttcca gataggcgcg gaccgtttca
     1441 ggcggtcggt ctgaattcat gggccattcc tccagtgccg cccttaagcg cgtcgtcgac
     1501 gaactcgcgc atgtcgcgcc aggcgaactg ccaatctgcc agcgccttcc gtccgtcgtc
     1561 cgtgatgttg tagtagcgcc ggggcggacc gaaggccgag gcttccaccc ggctttccaa
     1621 gaggcccaac gcgttcaacg agcgcaggac ggggtagatc gcgccctgct tgaagatcag
     1681 cccgccttcg ccctcggcct tcacggcttt ggcgatttcg tatccgtaca tctcccggcc
     1741 cgcgcgagcg agcacagcca ggagcacgag cgatgagagc ccggccatca gttccttacg
     1801 gaatttgcgc gtgaattgag attcttcagc cactgactaa acgcgccttt cttccgccct
     1861 ccaaactatt acgaactgat atctagcatg aacttcataa tatgcaaggg gtccctatag
     1921 cgaagttgaa aataattcga ttcgggtagc aaatggttaa tgggacgttt cgccgcagcc
     1981 gtgcctttgt gaagcacgga acgcctcgtc ccagcagctt actgttccgc tttgatcgcc
     2041 gtgagtacag ccgaggagcc ggccttgcct tcgatccgcg tttggccgaa cccattgttc
     2101 gccagaagct ggagcgtgcc cgcgatctcc tggcccgaac ggctgatcgc gttggggagc
     2161 cacttcggcc ccgccttctt gtgcgcccgc aagatcagca gcagcaggcc gcccggcttg
     2221 agcacgcgac gcacttcggc gatgtcgtgc gcgggatctt tccagaaatg aaaacaatgc
     2281 agtgccgccg ctttgtcgaa actgccgtcg ggccagggca gggcggacgc atcgcccaac
     2341 ttcaactcca cgcgcccggc cttgcgaaac ttcttggtgc gcttttccgc ggtccgcagc
     2401 atgagttcgg acggatcgac gccgccgaca aatccttcgt gcgccgcctt ggctagcttg
     2461 cgcgccagcc gtccggtgcc gaagccgatt tccagaagga tgtcgttctt ctgcatgtcg
     2521 agccgcttga gtgcgaaggc gtgtgcgcga gcgttgcgcc ggtccatgaa ccagcagaag
     2581 acgcggcccc agaagccatg aggacgcatc ggttgcggga ttttgccgcc gaccatattc
     2641 agctctcggc caggacgatc ttctcgacct ttacataatc cgttttcccg gtgccgagca
     2701 cggggatgtc gtcggtatga acaaggcggc gcggcacggc gatctcggca acgccgtggt
     2761 tttgcgccca gccgaccaaa tccacacgtt gggcttcggg gttcgtcgtc agcaggacga
     2821 tttgctcgcc cttgcgcccg tccggaacgg cgatggccgc atggctgtga tccggccaca
     2881 acgacgaggc gcaattttcg accaccgcca gcgacaccgt ttcgccgccg actttggcga
     2941 agcgtttcag gcggcccttg atcgagataa agccctcgct gtcgatggag accacgtcgc
     3001 ccgtgtcgtg ccagccgcct tccagcggaa cgacgacgcc aggattctcc ggcctgagat
     3061 agccgagcat gacgttcggc cctttcagga acagccgccc ggcattcgga ataccttcca
     3121 ccggttccag ccgggcctgc attccgggca gcagccggcc gaccgtaccg ggacgattgg
     3181 cgccgggctg attggcggcg gcgacgggag aggtttccgt cacgccgtag ccttcgatca
     3241 ggtcgatcgc gtacttcttg cgcagcagct ggcgcgtttc gtcgcgcacc ctttcggcgc
     3301 cgcacacggc aaggcgcagc gaattgagat cgccttgctc gcccgcgcgg gcatattgcg
     3361 acatgaaggt atccgtcgtg agcaggatcg tggcgcccgt ttccctgacg cggcgcacga
     3421 tttcgtgcgg ctgcagcggc gtgggatgga acgccgcctt gatccccagg atgagcggca
     3481 acagcgcgcc cgccaacagc ccgaagcaat ggaaagtcgg cagcggattg aacataacgt
     3541 cggttggata aaacgcgaca tgcgcacgca cctgctcgat attggacagc aggttcgaat
     3601 gcgacagcgc cacgcccttc ggttcgcctt cggtgccgga ggtgaacagg atgaccgccg
     3661 tcgattcatg tgccggccgc gaggtgacga ggcccggcac gaactgtccg atgcccgcaa
     3721 cggccttgtc gaagaccgac aaattctccc ggacgtcctc cagatagatc aactccgccg
     3781 tcatcgagat ttcagcggcc agcgcgtcca atttggccag ctcgatgaac cggcgcgccg
     3841 tgacgacccg tttgaccttg gcggtccgca atgcgctcaa aatggcggcg gcaccgttgg
     3901 tgaagtttcc catggtcggc acgcggccga aggcggaaac cgcaaagaag gaaacgatcg
     3961 cgccgatgct ggtcggcagc atgatgccga cggactcgcc ttttcttgtt ccctttttca
     4021 aggcatgacc gagcgccagc gacgcgcgca ggatctcgtc ataggtcacg acccgctcgt
     4081 cgccgtcgat gatggcgggg cgggtgcccc cgaaacggga ccgggcgtcg accagagcct
     4141 ggaatatcgt tttcttggta cgggtcatgt cgagcaggac gacgtcctcg cccgaagcgg
     4201 cagcgacggt agcgacagcc aattgctcct cccccggagg cgggcctcgc ccgctcttag
     4261 tattgtgaca caaactaacg gaacctagaa attgtcggta gagcaaccct tatgtagagg
     4321 atcgcggcca aggttttcaa ccggtcgggt aaaggcccgc gcccctgcgt cacccgcaaa
     4381 aggcgaaccg gcccggtaac cctgttcagc tctcggccag gacgatcttc tcgaccttta
     4441 cataatccgg tttcccggtg ccgagcacgg ggatgtcgtc ggtgtggaga atacggcgcg
     4501 gcaaggcgat ctcggccacg ccatggtttt gcgcccagcc gaccaaatcc acgcgctggg
     4561 cttcggggtt tgtcgtcagc agaacgattt gctcgccctt gcgcccgtcc ggaaccgcga
     4621 tggccgcatg gctgtgatcc ggccacaacg acgaggcgca attctcgacc accgccaagg
     4681 atacggtttc gccgccgact ttggcgaagc gtttcaggcg gcccttgatc gagataaagc
     4741 cctcgccgtc gatggagacc acgtcgccgg agtcgtgcca gccgccttcc agcggaacga
     4801 cgacgccagg gttctccggc ctgagataac cgagcatgac gttcggccct ttcaggaaca
     4861 gccggccggc gcccgggatt ccttccgccg gctccagccg cgcctgcatg cctgccatca
     4921 ggtggccgac ggttccggga cggttggcgc cgggctggtt ggcggcggcg aagggagacg
     4981 tctccgtcat gccgtagcct tcgatcaggt cgatcgcgta cttcttgcgc agcaactggc
     5041 gcgtttcgtc gcgcaccctt tcggcgccgc acaccgcaat gcgcaacgag ttcagatcgc
     5101 cttgctcgcc cgcgcgggca tattgcgaca tgaaggtgtc cgtcgaaagc aggatcgtgg
     5161 cgcccgtctc tctgacgcgg cgcacgattt catgcggccg cagcggcgtg ggatgaagca
     5221 ccgccttgat ccccagaaac agcggcgaca gcacgcccgc catcaacccg aagcaatgga
     5281 aggtcggcag cgggctcaac agaacatc
//
LOCUS       DASWPU010000631         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_48491,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000631 DASWPU010000000
VERSION     DASWPU010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48491"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3062
                     /locus_tag="VGC27_13475"
     CDS             <1..3062
                     /locus_tag="VGC27_13475"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0283617.1"
                     /translation="VEVWKDLPAQARKTIRDKKIKFFVIDGFKVAKPHAPTPELETRM
                     MGIAFIGAVCGHVDRIVGGVSHDAMLAKIREDLTKKFGAKGGAVVEGNLTVIREAFEA
                     TQKVDYTAPEFAAAEKAPAAATGRSVAISASMCRAAGQASSGGIFDRQYYDDIVAGPF
                     RDGTIAEAPVLPGTGLFMPSGSAAFKDKGLFRRDVPEYIADLCTGCMECALVCPDAAI
                     PNTVHDIHALMLTAIAQLDIAEPQREALRDQVHALSDAVRETYRHEKEPKPFHEIVAA
                     AAAELDIDNATLRRNLDKLAAALAVFPVAKTRPFFDAMEKAVPGSGGLYSAAVDPWKC
                     SGCLECIDVCGPHALVERHQDAALLETLQARFEFLSRTPNTPARFFEGATKPGGDTKR
                     LTLDRNNYYATTGGHGACRGCGEVTAIRLVTSANHAIHDARRKVHIREVEDVIERLNA
                     KLATVQPGENDPKRRERIVETIKTLEKRLYLLESGPTGNGPASMVIANATGCSSVYAS
                     TFPFNPYNDPWVNSLFQDTPAVAKGIFEGLSAEASDGIRALRVAKLELDDEYDPQVYD
                     RFFKMFSWADYTAEELAMLPTVISMGGDGATYDIGFGALSRLLATSTPIKVVVLNSGT
                     YSNTGGQASTSSLTGQDSDLARFGAVHKGKQEDRKELGLIAAFHPNVFVVQSCTALQG
                     HFMKSVMEYLNHNDSPAVLDVYAPCQAENGIGDSAGSRHGRMAVESRVSPVFVHDPRR
                     AASLRGRFVLDGNPDADKDWTTTSIEYVEDGATKLLEVPLTPADFALTEGRFKKQFRR
                     LAADAAGVPVAEYIDLGEAEKEGKTPFVWSTGADKKLVKYEVSPSLVHLVLERRKYWR
                     TLQYLSGMEVAKLDADHHAELEALQRQYKESLQARESSIDSIARAMSELAASSKAPPA
                     GGLGALMPGAAVAVQPAAPAAAEATNGSALVSLSEEDIAKCSNCKTCYQDLPELFEKT
                     KIVVDGVPKEVGHLIPGALSRVKITPELKSKVARVAANCDSEIVHEQ"
     gene            3052..>3538
                     /locus_tag="VGC27_13480"
     CDS             3052..>3538
                     /locus_tag="VGC27_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283618.1"
                     /translation="MSSEASTIAGAGPKELERYRQVATALETTRSEIEELEHSEAFEV
                     FQKQLGLLRKRLLNSPQSLRQVFVDDGTQAIVWEFKQDHLGEDFTKTLWNLLLRDDDM
                     STILMRFVWALPLKFKRKFIAALDAHLGGRYPMFKGLSKDWPGGNYIPPYIRPAEERK
                     ND"
BASE COUNT          676 a         1139 c         1151 g          572 t
ORIGIN      
        1 ccgtcgaggt ctggaaggat ttgccggcgc aggcccgcaa gaccatccgc gacaagaaga
       61 tcaaattctt cgtcatcgac ggatttaagg ttgccaagcc gcacgccccc acgccggagc
      121 tcgaaacccg catgatgggc attgccttca tcggcgcggt ttgcggccat gtcgaccgga
      181 tcgtgggcgg cgtctcgcac gacgcgatgc tggcgaagat ccgcgaggac ctgaccaaga
      241 agttcggcgc caagggcggc gcggtcgtcg agggcaacct caccgtcatc cgcgaagcct
      301 ttgaagcgac gcagaaggtc gactacaccg ctccggaatt cgcagcggct gaaaaggccc
      361 ctgcggccgc gaccggccgc agcgtggcga tttcggcctc gatgtgccgg gccgccgggc
      421 aggcgtcgag cggcggaatt ttcgaccgcc agtattatga cgacatcgta gccggtccgt
      481 tccgggacgg caccatcgcc gaagccccgg tgctgccggg caccggcctg ttcatgccgt
      541 cgggcagcgc cgcgttcaag gacaaaggcc tgttccgccg cgacgtgccc gagtacatcg
      601 ctgatctttg caccggctgc atggaatgcg cgctggtctg tccggatgcg gcgatcccca
      661 acacggtgca cgacatccat gccctgatgc tgaccgccat cgcgcagctc gatatcgcgg
      721 agccgcaacg cgaggcgctg cgcgatcaag tccatgcgct gagcgacgcg gtgcgcgaaa
      781 cctatcgcca tgagaaggag cccaagccgt tccacgagat cgtggcggcg gcggcggccg
      841 aactcgacat cgacaacgcc acgctgcgcc gcaacctcga taagctggcc gccgcgctgg
      901 cggtcttccc ggtcgccaag acgcggccgt tcttcgacgc gatggagaag gcggttcccg
      961 gcagcggcgg cctctattcg gcggccgtcg atccgtggaa gtgcagcggc tgtctcgagt
     1021 gcatcgacgt ctgcggaccg catgcgctgg tcgagcgtca ccaggatgcc gcgctgctcg
     1081 agaccttgca ggcgcggttc gaattcctca gccgcacgcc caatacgccg gcccgcttct
     1141 tcgaaggggc gaccaagccg ggcggcgata ccaaacggct gacgctcgac cgcaacaatt
     1201 attacgcaac caccggcggc cacggcgctt gccgcggctg cggcgaagtc accgcgatca
     1261 ggcttgtcac ctcggccaac cacgccatcc acgacgcgcg gcgcaaggtc cacatccgcg
     1321 aggtcgagga tgtgatcgag cggttgaacg ccaagctggc gacggtgcag cccggcgaaa
     1381 acgacccgaa gcggcgcgag cgcatcgtcg aaaccatcaa gacgctggag aagcggctgt
     1441 acctgctgga aagcggtccg accggaaacg gtccggcctc gatggtgatc gccaacgcga
     1501 ccggctgttc gagcgtgtat gcctcgacct tcccgttcaa tccctacaac gatccctggg
     1561 tcaacagcct gttccaggac actccggcgg tcgccaaggg cattttcgaa ggcctcagcg
     1621 cggaggcgag cgacggcatt cgtgcgttgc gcgtcgccaa gctggagctt gacgacgaat
     1681 acgatccgca ggtttacgac cgcttcttca agatgttcag ttgggccgac tacacggccg
     1741 aggaattggc catgctgccg accgtgatca gcatgggcgg cgacggagcg acctacgaca
     1801 tcggcttcgg cgccttgtcg cgtctgttgg ccacctcgac gccgatcaag gtcgtggtgc
     1861 tcaactccgg tacctattcg aacaccggcg ggcaggcatc gacctcgagc ttgacgggtc
     1921 aggattccga tcttgcccgc ttcggcgccg tccacaaagg caagcaggaa gatcgcaagg
     1981 aacttggtct gatcgccgcc ttccacccga acgtgttcgt ggtgcagagc tgcacggcgc
     2041 tgcaagggca cttcatgaag agcgtcatgg agtacctgaa ccacaacgac tcgccggcgg
     2101 tgctcgacgt ctatgcgccc tgtcaggcgg agaacggcat cggcgattcg gccggcagcc
     2161 gtcacggccg gatggcggtg gagagccggg tgagcccggt gttcgtgcat gatcctcgcc
     2221 gcgccgcgag cctgcgcgga cgcttcgtgc tcgacggcaa tcccgacgcc gacaaggact
     2281 ggacgaccac ctcgatcgaa tatgtcgagg acggcgcgac caagctgctg gaagtgccgc
     2341 tgaccccggc cgacttcgcg ttgaccgaag gccggttcaa gaagcagttc cgccgcctcg
     2401 ccgccgatgc ggccggcgtg ccggtcgccg agtacatcga tctcggcgag gccgagaagg
     2461 aaggcaagac gccgttcgtc tggtcgaccg gcgccgacaa gaagctggtc aaatacgaag
     2521 tgtcgccgtc gcttgttcat ctggtgctcg aacggcggaa atactggcgg accctgcaat
     2581 atctgagcgg gatggaggtg gcgaaactgg atgcggacca tcacgccgag ctggaagcct
     2641 tgcagcgcca gtacaaggaa tcgctgcagg cgcgcgaatc ctcgattgac tcgatcgccc
     2701 gcgcgatgtc cgaactggcg gcttcgtcca aggcgccgcc cgcgggcggc cttggggcgt
     2761 tgatgccggg agcggccgtt gcggttcagc cggcggcgcc ggccgcagcc gaggcaacca
     2821 acgggtcggc gctggtctcg ctttccgaag aggacatcgc caagtgcagc aactgcaaga
     2881 cctgctatca ggatttgccc gagctgttcg agaaaaccaa gatcgtcgtc gacggcgttc
     2941 ccaaggaagt gggccatctc attccgggag cgctctcccg cgtcaagatc acgccggagc
     3001 tgaagagcaa agtggctcgc gtggccgcca actgcgactc ggagatcgtc catgagcagt
     3061 gaagcatcga caatcgcggg ggccggcccc aaggagcttg agcgctatcg ccaggtggcg
     3121 accgcgctcg agaccacccg gagcgagatc gaggagcttg aacacagcga ggcgttcgag
     3181 gtcttccaga agcagctcgg cctgttgcgc aagcggctgc tgaacagtcc gcaatcgctg
     3241 cggcaggtgt tcgtcgacga cggcacccag gcgatcgtct gggagttcaa gcaagaccat
     3301 ctcggcgaag atttcaccaa gacgctgtgg aacctgctgc tgcgcgacga cgacatgagc
     3361 acgattttga tgcggttcgt ctgggctctg ccgctcaaat tcaagcgcaa attcatcgcc
     3421 gcgctcgacg cccatctggg cggtcgctat ccgatgttca aaggcctgtc gaaggattgg
     3481 ccgggcggca actacatccc gccctacatc cggccggccg aggaacgcaa gaacgact
//
LOCUS       DASWPU010000632         5088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_54388,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000632 DASWPU010000000
VERSION     DASWPU010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54388"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1880)
                     /locus_tag="VGC27_13485"
     CDS             complement(<1..1880)
                     /locus_tag="VGC27_13485"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEY0283619.1"
                     /translation="MNPLKDLERFGQAPWLDYVSRGLLRGGELALLVERDGVKGVTSN
                     PSIFEKAIGHSDEYDEEIGTLLQRGCADAGAIFRQLAVADIRAAADVLAPVYEATKAA
                     DGYVSMEVSPYIAHDTDATIAEAREIWSEVARDNLMVKVPGTPEGLPAIRQLTSEGVN
                     INVTLLFSKAVYIDVVEAYIAGLEGLPKTHDLSRVAGVASFFVSRIDAKIDAKLEDML
                     KTAPAAERARLESLRGKAAIANAKLAYREYERLFSGPRWEALAKRGARPQRLLWASTG
                     TKSKTYSDVLYVEELIGPDTVNTMPPETMNAFRDHGRAQASLQTGLADAEKTLADLKA
                     VGISLDEVTDWLVGDGVDKFADAADLLYGAIAEKCEKLSGSKVKIGYDLAEKAEGVNA
                     ELKSWAKKGNIRRLWAKDATLWSGADESKWLGWLDIAKRENAGLAALQDFAERVKKKG
                     WSDVVLLGMGGSSLGAEVLREVLGGQASAPRFHILDSTDPGEIGTLDKAIAPATTLFI
                     VASKSGTTLEPNIFKDYYFDRVAHAVGRDRAGGHFVAITDPGSLLEKTAAQDGIADVF
                     HGDPTIGGRYSVLSNFGLVPAAAIGIDLKSLIAEARWMQAACGPLSPPANNPGARLGL
                     ALG"
     gene            2097..2951
                     /locus_tag="VGC27_13490"
     CDS             2097..2951
                     /locus_tag="VGC27_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0283620.1"
                     /translation="MKLVTFTGGADAELGVVDGDEVVALSRAVPRLATGMIDLIARWS
                     EAEGEVRRAVQSGPRLSLASVRLLPPVPRPGKILAIGLNYADHILETGHEMPAAQVWF
                     CKQPTAANGPYDPVRLPKVSVQVDYEAELVAVIGKGGRAIPKERAPASVFGFCCGNDV
                     SVRDWQFATTQWMLGKSFDTHAPFGPWITTADEAGDPHTLGVRSFVNGEKRQDSNTRH
                     FVFGLWDQIEHVSKVMTLEPGDLLFTGTPGGVGMAMKPPAYLRAGDRVRVEVDGLGFL
                     EAVCRPEE"
     gene            3102..4877
                     /gene="sppA"
                     /locus_tag="VGC27_13495"
     CDS             3102..4877
                     /gene="sppA"
                     /locus_tag="VGC27_13495"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY0283621.1"
                     /translation="MIAFLRWAGSIVAGTFNGLLKFALAIVVVFVAIVVIGLARGDGL
                     PDNILLSLDLRKALADSSPSEFTLGARSVTVMDIVLGLDAAQRDKRVKGVFVRAGSGN
                     LPVSQAEEIGSALKRFRTSGKFVIAHAQGFDGSGLGDYLAAASASQIWMQPKSPFMAS
                     GEGGGKLFLRGLLDKIQAEPQIAKRAEYKSGADTYMEKNMTAADREQLTALMQSWYDN
                     AVAGAAADRKLDAAALKAAFEASPQFTEDVKKTGLIDKVGYDDDALAAATKLAGAKAQ
                     SVPLGQYVRVKSAASRYGKGQRIALVEAAGEIVEGTASGSLFGDNSVIAGDDMARAIR
                     EAAKDKGIKAIVLRVDSPGGSATASDQILDAVKKAQAAGKPVIVSMGQVAASGGYYIA
                     ASADKIVAEPGTVTGSIGAYTGKVSIGKSLSLIGVGTDQVGIGKNALMNSALTPYTPE
                     QWAAVDAQAETIYADFTQKVADGRKLPLAKVQEIARGRVWSGADAKAHGLVDQLGGFW
                     TAVDLAKKQARIAASERVVFERYPRRKGFFEAVNEFFGGPSEEVRAVGTFIALMNAPL
                     AREAAAAVKSAPRGGVELRAVNLPR"
BASE COUNT          859 a         1563 c         1737 g          929 t
ORIGIN      
        1 cccagcgcca gccccagacg cgcgcccgga ttgttggcgg gagggctgag cggcccgcag
       61 gcggcctgca tccagcgggc ttcggcgatg aggctcttga ggtcgatccc gatggcggcg
      121 gccgggacca ggccgaaatt cgagagcacg gaatagcgcc cgccgatcgt gggatcgccg
      181 tggaagacgt cggcgatgcc gtcctgtgcc gccgtctttt ccagcaagga gccgggatcg
      241 gtgatggcga cgaagtgccc gccggcgcgg tcgcggccga ccgcatgcgc gacgcgatcg
      301 aaataataat ccttgaagat gttgggttcg agcgtggttc cggatttgga ggcgacgatg
      361 aaaagcgtgg ttgccggggc gatcgctttg tcgagcgtgc cgatctcgcc ggggtccgtg
      421 gaatcgagga tgtggaagcg cggggccgag gcttgccccc cgagaacctc gcgcagcacc
      481 tccgcgccca ggctcgatcc gcccatgccg agcagaacga cgtcgctcca gcctttcttt
      541 tttacgcgct cggcgaaatc ctgaagcgcg gcgagaccgg cgttctcgcg cttggcgatg
      601 tcgagccagc ccagccattt gctttcgtcg gctccgctcc acagcgtcgc gtccttggcc
      661 cagaggcggc ggatattgcc tttcttggcc caggatttca gttcggcgtt gacgccctcc
      721 gccttttccg cgaggtcgta gccgatcttc accttggagc cggacagctt ttcgcatttt
      781 tccgcgatgg cgccgtagag caggtcggcg gcgtcggcga atttgtctac gccgtcgccg
      841 accagccaat ccgtcacttc gtccagcgag atgccgacgg ctttcaagtc ggcgagcgtc
      901 ttttcggcgt ccgcgaggcc cgtttgcagc gacgcctgcg cgcggccgtg atcgcggaag
      961 gcgttcatgg tttcgggcgg catcgtattg accgtgtccg gtccgatcag ctcttcgacg
     1021 taaagcacgt cggaataggt tttgctcttg gttccggtcg aggcccacaa caaccgttgc
     1081 ggacgcgcgc cgcgtttggc cagcgcctcc cagcgcggcc cggagaacag gcgctcatat
     1141 tcccggtagg ccagtttcgc gttggcgatg gccgctttcc cgcgcaggct ttccaggcgc
     1201 gcccgctcgg ctgccggggc cgtcttgagc atgtcttcga gcttggcgtc gatcttggcg
     1261 tcgatgcggc tgacgaagaa gctcgccacg ccggcgacgc gggaaaggtc gtgcgtcttc
     1321 ggcaagcctt ccagtccggc gatataggct tccaccacgt cgatatagac cgccttggag
     1381 aacagcagcg tgacgttgat gttcacgcct tccgaggtga gctggcggat ggcgggcagg
     1441 ccctccggcg ttccgggcac tttcaccatc agattgtcgc gggcgacctc ggaccagatt
     1501 tcgcgcgcct cggcgatggt ggcgtcggtg tcgtgggcga tgtagggcga gacttccatg
     1561 ctgacatagc cgtccgccgc tttggtggcc tcatagaccg gggccagaac gtccgccgcc
     1621 gcgcggatgt cggcgacggc cagttggcgg aagatcgcgc cggcgtcggc gcagccgcgc
     1681 tgcaacaagg tgccgatttc ctcgtcatat tcgtcggaat ggccgatggc tttctcgaag
     1741 atcgaaggat tggaggtaac ccctttgacc ccgtcccgtt ccaccagcag cgccagttcg
     1801 ccgccccgaa gtaagccccg gctgacataa tcgagccaag gcgcctgtcc aaatcgttcg
     1861 aggtctttca acgggttcat gttcggttcg ctgtcctatg ttttggtgtt gtcaggaaat
     1921 agcaggatcg gccggctggc gcagcctccg gccggggcag tctagtcatc gcggccgcgg
     1981 aacggaataa gaattgagcg tgccgcaccg tcgcaacgat tccctatcgg gcggtttccg
     2041 cagccttttc gttgctgtag gtacatggcg gcattggcaa gggaaggctg gcggccatga
     2101 aactcgtgac tttcaccggc ggcgcggacg ccgaactggg cgtcgtggac ggcgacgagg
     2161 tcgtagcgct ttccagggcc gtgccgcggc ttgccacggg gatgatcgat ctgatcgccc
     2221 gctggagcga ggccgagggc gaggtccggc gcgcggtcca gtccggcccg cggctgtccc
     2281 tggcctcggt gcggctgctg ccgccggtcc cgcgccccgg caagattctc gccatcggcc
     2341 tcaattacgc cgaccatatc ctcgaaaccg gccatgagat gccggccgcg caggtgtggt
     2401 tctgcaagca gccgacggcc gcgaacgggc cctacgatcc ggtccgcctg ccgaaggttt
     2461 ccgtacaggt ggattatgaa gcggagctgg tcgcggtgat cggcaagggc gggcgtgcca
     2521 tccccaagga gcgcgcgccg gcttccgtgt tcggcttttg ttgcggcaac gacgttagcg
     2581 tgcgggattg gcagttcgcc acgacgcaat ggatgctcgg caaatccttc gacacccatg
     2641 caccgttcgg gccgtggatc accacggccg acgaggcggg cgatccgcac acgcttggcg
     2701 tgcgcagctt cgtcaatggc gagaaacggc aggattcgaa cacgcgccat ttcgtcttcg
     2761 gcctctggga ccagatcgag catgtttcca aggtgatgac cctcgagccg ggcgaccttt
     2821 tgttcacggg cacgcctggg ggcgtcggca tggccatgaa gccgccagcc tatctcaggg
     2881 ccggcgaccg ggtccgggtc gaggtggacg ggcttggctt cctcgaagcc gtctgccggc
     2941 cagaggaata aattcagcgt tactttgatg tttggtgtga agagcaaggg ttacggcggg
     3001 gcagccgtat cccggccatt cacaatcctt aatatgctcc ccatgtataa ggcgaatgcg
     3061 gacggcgctg gccggttccg ggacgaagga tggattgcat catgatcgct tttttgcgct
     3121 gggccggttc catcgtggcc ggaacgttta acgggcttct caaattcgcg ctggccattg
     3181 ttgttgtctt tgtggcgatc gtggtgatcg gactggcgcg cggtgacggc ctgccggaca
     3241 acatcctcct gtcgctggac ctgcgcaaag ccctggcgga ttcgtcgcct tcggagttca
     3301 cgctgggagc gcggtccgtc acggtgatgg acatcgtgct cggcctggat gccgcccagc
     3361 gcgacaagcg cgtcaagggc gtgttcgtaa gggcgggaag cgggaacctt ccggtctcgc
     3421 aagccgaaga gatcggcagc gcgctcaagc gcttcagaac ctcgggcaag ttcgtgatcg
     3481 cgcatgcgca agggttcgac ggcagcgggc tgggcgatta tctggcggcc gcttccgcca
     3541 gtcagatctg gatgcagccc aagagcccgt tcatggcgtc cggcgagggc ggcggcaagt
     3601 tgttcctgcg tggcctcctc gacaagatcc aagccgaacc gcagatcgcc aagcgcgccg
     3661 agtataagag cggcgccgac acgtacatgg aaaagaatat gacggcggcg gatcgcgagc
     3721 agctcacggc gctcatgcag tcctggtacg acaatgcggt ggcgggcgcg gcggccgacc
     3781 gcaagctcga tgcggccgcg ctcaaggccg ccttcgaggc cagcccgcaa ttcacggaag
     3841 acgtgaaaaa gacgggtttg atcgacaagg tcggctacga cgacgatgct ctggccgccg
     3901 cgaccaagct tgccggcgcg aaggcgcaaa gcgttccgct ggggcaatat gtgcgcgtca
     3961 agagcgcggc gtcgcgttac ggcaaaggcc agcgcatcgc tctggtcgaa gcggcaggcg
     4021 aaatcgtcga aggcactgcc agcggcagtc tgttcggcga caattcggtg atcgcgggcg
     4081 acgacatggc gcgcgccatc cgcgaggccg cgaaagacaa gggcatcaag gcgatcgttc
     4141 tgcgcgttga ttcccccgga ggctcggcga cggcttccga ccagattctc gatgcggtga
     4201 agaaggcgca ggcggccggc aagccggtga tcgtcagcat ggggcaggtc gcggcctcgg
     4261 gcggctatta catcgccgcc tcggcggaca agatcgtcgc cgagccgggc accgtcaccg
     4321 gatcgatcgg cgcctacacg ggcaaagtgt cgatcggaaa gtcgctcagt ctgatcggcg
     4381 tgggaaccga ccaggtcggc atcggcaaga acgcgctgat gaattccgct ctgacgccct
     4441 acacgccgga acagtgggcc gcggtagacg cccaggccga gacaatctat gcggacttca
     4501 cccagaaagt ggcggacggg cgcaagctgc cgctcgccaa ggtccaggag atcgcgcgcg
     4561 ggcgcgtgtg gtcgggcgcg gacgccaagg cgcacggcct ggtggatcag ctcggcggct
     4621 tctggactgc ggtcgatctc gccaagaagc aggcgcgcat cgccgcttcg gaacgcgtcg
     4681 tgttcgagcg ctatccgcgc cgcaagggtt tcttcgaggc ggtgaacgag ttcttcggcg
     4741 gcccctccga agaggtgcgc gcggtgggaa ctttcatcgc cctgatgaat gcgccgctcg
     4801 cgcgcgaagc ggcggcggcg gtgaaatcgg cgccgcgcgg cggcgtcgaa ctgagggccg
     4861 tcaacctgcc gcggtaagac agggcgggtt cgcgggacgc ggacgaaaaa ggaaagggcg
     4921 gagctttcgc ttcgcccttc ttcttgccgg catggcgtgc tagacgcgtc gcttcgctcg
     4981 gcccctgccc gacagagtaa cattataaat aatcggccgg caggcgatca taaggcttag
     5041 ggacgcaatg ccatgaccgg tcaaaatcgt gtcctcctcg ccatagat
//
LOCUS       DASWPU010000633         6831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_85532,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000633 DASWPU010000000
VERSION     DASWPU010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85532"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(440..1306)
                     /locus_tag="VGC27_13500"
     CDS             complement(440..1306)
                     /locus_tag="VGC27_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0283622.1"
                     /translation="MSFVKILVPVAGASRDRFALATAFAAAKPFNAHVVAMFVHADPR
                     EAVPYGELPLSSDVVQDLIDTAADLELAASKAARSTLATLAADAGIRIVGAPQKADAV
                     TVSYDEMTGHLSRALDREALLCDVVVFPPIRAADDSEIQDGFIRALTKLERPVLLSAE
                     TAPVEIGKKVAVGWDGGVACAHALMSALPYLEKAEAIELFTVQNGSHQGRGVECAAAY
                     LALHGLSSSQRVLKRGERSVAEVLLETAAADGCDLLVAGGYGHSRLMESVFGGVTEHI
                     VSHPKLPVFMMH"
     gene            complement(1418..2164)
                     /locus_tag="VGC27_13505"
     CDS             complement(1418..2164)
                     /locus_tag="VGC27_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0283623.1"
                     /translation="MQRRVADLEMSGGSVLPSGEPLPVRPAACEDGCRFFMEEACVRK
                     ADMDKVGVTVKFGRNETIYSKAEEARYSYKVIEGAVRLSRIFSDGRRQIVNFFLPDET
                     FGIELADEYTSTAEAVGEVVALRCPRLCIAQLTEGDPDIGQKRLAMLSKSLSAAEQHV
                     AMLGHQSAKERVASFFLALEMQRRAEAEHTLDLPLSRQDIADYLGLTIETTCRALSEL
                     KRQRIIDAPSRRRIVIRDLAGLKAISEGGG"
     gene            complement(2296..4983)
                     /locus_tag="VGC27_13510"
     CDS             complement(2296..4983)
                     /locus_tag="VGC27_13510"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEY0283624.1"
                     /translation="MSIRNLDAFFHPASIALIGASARPNSVGAVVASNLFTGGFKGPI
                     MPVNPKSLAINGVLAYPDVAHLPITPDLAIIATPPDSIPGIVAELAERGTRAAVVVTA
                     GLRDIKDASGVSLEQKMLAAAKPKLLRIAGPNCIGIISTPAGINASFAEAKPRKGKVA
                     FVAQSGAMIATMLDWASTRGLGFSHLVSMGDQADVDFGDMLDYMATDPDTDSIVLYIE
                     AAGHARDFNARKFMSAARAAARVKPVIAIKAGRSAAAAKAASSHTGALAGVDGVYDAA
                     FQRSGILRAMDLDEIFDAVETLSMRPRVTGDRLMIVTNGGGAGVMAVDALSDLNGTLA
                     DISQQTIEKLDAVCPPTWSRGNPVDIIGDAPGKRFSDTLDILAEAPDVDGVIVLYCPT
                     AVASSLEGAQAVIDSTKGTQRPVLTNWLGSSEETNEARRAFQKAGIPTYETPEKAVRG
                     FMHMARYARGQTVLLEVPPSASEDFVPDEAKARAIVAEAMAEEQVWLDAPRLQMLFDC
                     YAIPIARSAKAVTPDDVAKKSAEFGAPVVIKILSPDITHKSDVGGVALNLEDPAAAKA
                     AAEAMLVRVAKNAPKAKIDGFLVQEMIRRPGAYELILGMAVDNTFGPFLLFGQGGVSV
                     EVVGDKALALAPINSALAHDMISRTRIYRQLKGFRDRPPVALNDIAAVLVRLSQLVCD
                     FPEIVELDVNPLLADSKGVISVDARVKLAKCKPGPRDARLAIRPYPRELEHKEKIPGL
                     GEFFLRPVRPQDAASFTRFFARLQPDDVRLRFFSPLKSLPANLLARLTQIDYDRDMAF
                     VLLDADDELMGVAHFSSDPDKLRGEYSVLVRSDLKGNGIGRALMQQTIAYAKRAGVGE
                     IFGDVLEENAMMISLCKELNFTMAPLAGSKGIVRTSLVL"
     gene            complement(5043..5636)
                     /locus_tag="VGC27_13515"
     CDS             complement(5043..5636)
                     /locus_tag="VGC27_13515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0283625.1"
                     /translation="MQHALIVAHPNPGSFVASLAMAYGEAVEGLGQKTIMRDLYGMSF
                     DPCLKADELPTAAGFAPRADVAGERVLLRGVDVFALFYPFWLNSPPAMLKGYLERVFG
                     MGFGYSKGSGGNEPLLRGRKLISFTASGAPAEWVKKTGALEAVRALFDDHVAAICGLQ
                     VIEHLHFGGIVPGIRGDVVMRHQQSVRDLAARQFGSH"
     gene            5971..6246
                     /locus_tag="VGC27_13520"
     CDS             5971..6246
                     /locus_tag="VGC27_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283626.1"
                     /translation="MPNEDVQKLSDDITELRADFTRIAETLADLVRLRGQEAAAKFQG
                     SAEETWADAKQHFEGVKKKIKDEPVTAAAVAFGVGLILGILLFGGRR"
     gene            6312..6707
                     /locus_tag="VGC27_13525"
     CDS             6312..6707
                     /locus_tag="VGC27_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283627.1"
                     /translation="MAAALLVAAVGIGATVVLLCLALYSLLLTLLAAPPLAALAAAAL
                     MFFLSLFVIFLAGALSRGIARRERERRAQRGGIGVALCVELGKILGEDAQKFITRKPV
                     LTLVLAVAGGFAVSVSPRLRALLLTLLRY"
BASE COUNT         1285 a         2201 c         2159 g         1186 t
ORIGIN      
        1 gctggaaatt cggtcaggtc ctccgggaga gagattctac accgcgtatt cgtttttgac
       61 tttccggacc cccctccccc taaatgaccc tgcccaattg aacctcccag aaccggaggg
      121 tgccccaccc ggaaccgggg acccatcgca cgcagtatag ggcaagccgg cagatcgtgg
      181 ataggaaacg agacgcaagc gcaatcggcg ctctcctctc ccattcaagc cgcacaatca
      241 tcgcaactgc cgcaacggca caatgtgcga gatgccatcg caaggccgga atcgcccgac
      301 cccaatcgtc gcgaaatcat gctgcgccca tcccccgaca atctgcattc cgtcgccgaa
      361 ttctctccca tcacctggat ggcctgcatt cgcaggccat gacagaggag tatcggggcg
      421 acgcagacgc ctgccgctat cagtgcatca tgaagacggg aagtttcgga tgcgagacga
      481 tgtgttcggt gaccccgccg aagaccgatt ccatcagccg gctgtgcccg tagccgccgg
      541 ccaccagcag gtcgcagccg tcggccgccg cggtttccag caaaacctcg gccacgctcc
      601 gctcgccgcg cttcagcacg cgctgcgaag aggaaaggcc atgcagcgcc agataggccg
      661 cggcgcactc cacgccgcgc ccctgatggg agccgttctg gaccgtgaaa agctcgatcg
      721 cttcggcctt ttccagataa ggcagcgccg acatcagcgc atgggcgcag gccacgccgc
      781 cgtcccagcc cacggccacc ttcttgccga tctcgacggg cgccgtctcg gccgacagca
      841 gcaccggacg ctccagcttg gtcagcgcac ggataaatcc gtcctggatt tcggaatcgt
      901 cggccgcgcg gatgggcgga aacaccacca catcgcagag cagcgcctcg cgatcgagcg
      961 ccctggacag atggccggtc atttcgtcgt aggaaaccgt caccgcatcc gccttctgcg
     1021 gcgcgccgac gatacggatg cccgcatccg cagccagggt cgcgagggtc gagcgcgcgg
     1081 ccttcgacgc ggccagttcc agatcggcgg cggtgtcgat caggtcctgc acgacatccg
     1141 acgacagagg aagctcgcca tacggtacgg cctcgcgcgg atcggcgtga acgaacatcg
     1201 ccacgacatg ggcgttgaag ggcttggcgg ccgcgaaggc cgtggccaaa gcaaaacgat
     1261 cgcggcttgc tccggcgacc ggcacgagaa tcttgacgaa cgacatggcg cctccggatg
     1321 cagctcctac ctcgcccgca cagcaaaacc gaacgcgcga aaaaacgcat tgagacttgt
     1381 caatattgag ccttgtcaat gaacggccgg cggcgattca gccgccgccc tcggagatcg
     1441 ccttcaggcc ggcaaggtcg cggatcacga tccgcctgcg gctcggcgcg tcgatgatcc
     1501 gctggcgctt gagctcgctc aaggcgcggc aggtggtctc gatggtcagc ccgagataat
     1561 cggcgatgtc ctggcggctc agcggcaggt cgagcgtgtg ctcggcttcc gcgcgccgct
     1621 gcatttccag cgccaggaag aacgaggcga cacgctcctt ggcgctctgg tggccgagca
     1681 tggcgacgtg ctgttcggcg gcggaaagac tcttgctcaa catcgccagc cgcttctggc
     1741 cgatgtcggg atcgccttcg gtcaattggg cgatgcaaag gcgcggacag cgcaaggcga
     1801 cgacctcgcc caccgcttcg gcggtcgagg tatattcgtc tgcaagctcg ataccgaagg
     1861 tctcgtcggg aagaaaaaaa ttgacgatct ggcggcggcc gtcggagaaa atgcgcgaca
     1921 ggcgcaccgc gccttcgatc accttgtagg aatagcgcgc ctcttcagcc ttggaataga
     1981 tcgtctcgtt gcggccgaat tttacggtga cgccgacctt gtccatgtcg gccttccgga
     2041 cgcaggcctc ctccatgaaa aagcggcagc catcctcgca ggcggcagga cgcaccggga
     2101 gcggttcgcc cgacggcaaa accgaaccgc cgctcatttc gaggtcggca actctgcgct
     2161 gcatagggcc acatgtctcc cggcaaggac aattttgccg ggatgatagc cttgcagcgc
     2221 gcggttaagc aaaggcccgc cgtttcccgc acggagccta tgccccgcgg gcccgcccga
     2281 gtcttcgcct tagggtcaga gaaccaaact tgtccttacg atacctttgg atccggccag
     2341 cggcgccatg gtgaagttga gttctttgca cagcgagatc atcatcgcgt tctcttccag
     2401 cacgtcgccg aagatttcgc cgaccccggc gcgcttggca taggcgatgg tctgctgcat
     2461 cagcgcgcgt ccgatgccgt tgcccttcaa gtcgctgcgc accagcaccg aatattcgcc
     2521 ccgcagcttg tcgggatcgg aggagaagtg cgccacgccc ataagctcat catcggcatc
     2581 aagaagaacg aacgccatat cgcggtcgta gtcgatctgc gtcaggcggg cgagcagatt
     2641 ggccggcagg ctcttgagcg gcgagaagaa gcgcaggcgg acgtcgtcgg gctgcagccg
     2701 cgcgaagaaa cgcgtgaaac tcgccgcatc ctgcgggcgc accggacgca ggaagaattc
     2761 tcccaggccg gggatcttct ctttgtgctc gagttccctc ggataaggac ggatggcaag
     2821 gcgggcatcg cgcggaccgg gcttgcactt ggccagtttc acgcgcgcgt ccaccgagat
     2881 cacgcccttg gaatcggcca ggagcgggtt gacgtcgagt tcgacgatct ccgggaaatc
     2941 gcagaccaac tgcgacagcc gcaccagaac ggcggcgatg tcgttgagcg ccacgggcgg
     3001 gcggtcgcgg aagcccttga gctggcgata gatgcgcgtg cgggaaatca tgtcgtgggc
     3061 gagcgcgctg ttgatcgggg ccaaggccag ggccttgtcg ccgaccactt ccaccgagac
     3121 gccgccttgg ccgaacagca ggaacggacc gaaggtgttg tccaccgcca tgccgagaat
     3181 caactcatag gcacccggac ggcggatcat ttcctgaacc aggaagccgt cgatcttggc
     3241 cttcggcgca ttcttggcga cgcgcaccag catcgcttcg gcggcggcct tggcggcggc
     3301 agggtcttcc agattcagcg ccacgccgcc gacatcggac ttatgggtga tgtccggcga
     3361 cagaatcttg atcaccaccg gagcgccgaa ttcggccgac ttcttcgcga catcgtcggg
     3421 cgtcaccgcc ttggcggagc gcgcaatcgg aatggcgtag caatcgaaca gcatctgcag
     3481 acgcggcgcg tcgagccaga cctgctcttc cgccatggct tccgccacga tggcgcgcgc
     3541 cttcgcttcg tcgggcacga aatcctcgga cgccgacggc ggaacttcca gcagcacggt
     3601 ctggccgcgc gcataacggg ccatatgcat gaagccgcgc accgcctttt ccggcgtctc
     3661 gtaagtcggg atgccggctt tctggaaggc acggcgggct tcgttggttt cttcgctcga
     3721 tcccagccag ttggtcagca ccgggcgctg ggtgcccttc gtcgaatcga tcacggcctg
     3781 ggcgccttcc agactcgagg cgacggccgt cgggcaatag agcacgatca cgccgtcgac
     3841 gtcgggcgcc tcggcgagga tgtcgagcgt atccgaaaag cgtttgccgg gggcgtcgcc
     3901 gatgatgtcc accggattgc cgcgcgacca agtgggcgga cacaccgcgt ccaatttttc
     3961 gatcgtctgc tggctgatgt cggcaagcgt gccgttcagg tcggacaaag cgtccaccgc
     4021 catcacgccg gcgccgccgc cgttggtgac gatcatcagc cggtcgccgg tcacgcgcgg
     4081 acgcatcgac agcgtttcca ccgcgtcgaa aatctcgtcg aggtccatcg cgcgcaggat
     4141 gccggagcgc tggaaggcgg cgtcgtaaac cccgtccacg ccggccagcg cgccggtgtg
     4201 cgaagacgcg gccttcgccg ccgcggcgct gcggcccgcc ttgatggcga tgaccggctt
     4261 gacgcgcgcc gccgcgcggg cggccgacat gaacttgcgc gcattgaaat cgcgcgcatg
     4321 cccggccgcc tcgatgtaga gcacgatcga atcggtgtcc ggatcggtcg ccatgtagtc
     4381 gagcatgtcg ccgaaatcga cgtcggcctg gtcgcccatg gagacgagat gggaaaaacc
     4441 gagaccgcgg gtggaggccc aatcgagcat ggtggcgatc atggcgccgg attgcgccac
     4501 gaaggcgacc ttgcccttgc gcggcttggc ctcggcgaac gacgcgttga tgccggccgg
     4561 ggtcgaaatg atgccgatac agttcgggcc cgcaatccgc aacagcttgg gtttcgccgc
     4621 ggcgagcatt ttctgttcga gcgaaacgcc cgacgcatcc ttgatgtcgc gcagtcccgc
     4681 ggtgaccacc acggcggccc tggtgccgcg ctcggccagc tcggcgacaa tgcccggaat
     4741 gctgtcggga ggcgtggcga tgatcgccag atcgggggtg atcggcagat gcgccacgtc
     4801 cggataggcg agcacaccgt tgatcgccag gctcttcggg ttgaccggca tgatcgggcc
     4861 tttgaagccg ccggtgaaaa ggttggacgc caccacggcg ccgaccgaat tcggccgggc
     4921 gctggcgccg atcagagcga tacttgcggg atggaaaaag gcgtcgagat tgcgaattga
     4981 catgtgtcac atccctaaaa aagcagcccc tactaaccca ccggccgacg cattactacc
     5041 ggtcaatgtg agccgaattg tctcgctgcc aggtcccgta cggactgctg gtgacgcatc
     5101 acgacatcgc ctcgaatacc gggcacaatt ccgccaaaat gcaggtgttc aataacctgt
     5161 aggccgcata tcgccgccac atgatcgtca aataacgcac ggacggcttc cagggcgccg
     5221 gttttcttca cccattcggc cggggcgccg gaggcggtga agctgattaa tttccggccg
     5281 cgcaacaagg gttcgttgcc gccgctgcct ttgctatagc caaagcccat cccgaatacg
     5341 cgctcgagat agcccttcag catggccggc ggggagttca accagaacgg atagaacagc
     5401 gcgaagacat cgacgcctcg cagaagcacc ctttcgccgg cgacgtcggc gcgcggcgca
     5461 aatccggccg ctgtcggcag ctcgtcggcc ttcaagcaag gatcgaacga catgccatag
     5521 agatcgcgca tgattgtttt ctggcccagc ccttccaccg cttcgccata ggccatggcc
     5581 agagaggcca caaagctgcc cggattgggg tgggcgacga tcagcgcatg ctgcatgacc
     5641 agctttcctg ctttgccgtt tcgtatattg acagtcattc tgaaaaagcg ccaacgaggg
     5701 cgcattgcgc aatgtcaaat atcccgcaga cgcctcggtc aagaggttac ggacgcgtgc
     5761 ggtgtttacc catgccgaga gcgctttgcg gctgccgtca ccccgtccgg ttcgcgcccc
     5821 ggaggcggac ctggccgagg cccgcaggcc ggcgtccgcc cagcggccat ccgctgcccg
     5881 cggccggggg cggccagagc agcgacttga ctattgcccg gtcaaggaca acctatagaa
     5941 aattaacttc tgtcagacag gatcgctgcc atgccaaacg aagatgtcca aaaactaagc
     6001 gacgacataa ccgaattgcg ggccgatttc acgcgcatcg cggagacgct ggctgatctg
     6061 gtgcgtctgc gcggtcagga agcggctgcc aagttccaag gatcggccga ggaaacctgg
     6121 gctgacgcca agcaacattt cgaaggcgtg aagaagaaga tcaaggacga acccgtgacg
     6181 gccgccgcgg tggctttcgg ggtcggcctg atcctcggga tcctgctgtt cggcggacgg
     6241 cgttagacca tcctttcggc aatcctggaa gggccgggcg gatgagagaa ttgccgttgc
     6301 gcatcgcaat catggctgca gcgctgctgg tggctgcggt cggcatcggc gcgactgtcg
     6361 tgttgctctg cctggcgctc tattccctgc tgttgacact gctcgccgcg ccgccgctgg
     6421 ctgccctggc ggcggcggcg ctgatgttct tcctgtcgct gttcgtgatc ttcctggccg
     6481 gagcgctctc aagggggatt gcccggcggg agcgggagcg ccgcgcccag aggggcggca
     6541 tcggcgtcgc cctctgcgtc gaactgggaa agatcctggg cgaagacgca cagaaattca
     6601 tcacccgcaa gccggttctg acgctggtct tggcggtggc cggcgggttt gccgtcagcg
     6661 tcagcccgcg gctgcgcgct ctgcttctga cgctgctgag atactagagc agcgggcgtt
     6721 ccgatgaaat cgtcacgccg ctctatgccc ttgttttgtc gcaacgctta tgcggaaaac
     6781 cgcacacact tttccgcgcg ttgctctaaa cgaaatccga ggttccaatg a
//
LOCUS       DASWPU010000634         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_73152,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000634 DASWPU010000000
VERSION     DASWPU010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73152"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..532)
                     /locus_tag="VGC27_13530"
     CDS             complement(167..532)
                     /locus_tag="VGC27_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009802170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0283628.1"
                     /translation="MPVLKVGIGEAGESRKAMPGDPTVWFPSTESFARLLTADNRTML
                     RLIAERAPESLDELVELTGRAKPNLSRTLHRLASYGIVRTERRGRKIVPTLLCDRIEL
                     ILPLTGHGLPGATGKGHRA"
     gene            complement(612..779)
                     /locus_tag="VGC27_13535"
     CDS             complement(612..779)
                     /locus_tag="VGC27_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283629.1"
                     /translation="MGYPWPFPGRPPKHDLSTWTVTDDWPHPVPVTETEIEVFEQWFD
                     DLFDELSGPGD"
     gene            complement(850..1065)
                     /locus_tag="VGC27_13540"
     CDS             complement(850..1065)
                     /locus_tag="VGC27_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HEY0283630.1"
                     /translation="MLVADRIVRMKTVLARTGLSRSTIYRKMTEGTFPARIKISVNGA
                     GWRESDINRWVANPVTWRPRSEFDFDD"
     gene            complement(1196..>2912)
                     /locus_tag="VGC27_13545"
     CDS             complement(1196..>2912)
                     /locus_tag="VGC27_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0283631.1"
                     /translation="SGSGIASRTIASMEHGWGQGRDALTSRDVLVIDEAGMVGTRQLE
                     RVLSHAADAGAKVVLVGDPQQLQSIEAGAAFRSIFERHGGAEIGEVRHQREDWQRDAT
                     RELAAGRTGEAIHAYDAHGMVHAAETREQARDDLIEGWNRERQASPDKSRIILTHTND
                     EVRALNEAARERLREAGDLGDDVRITVERGERSFASGDRVMFLQNERGLGVKNGTLGT
                     IEQVSARSMSVRTDNRRSISFDLKDYDRIDHGYAATIHKAQGMTVDRTHVLATPGLDA
                     HGSYVALSRHCDGMDLHYGRDDFADQDRLIRTLSRDRAKDMASDYERADPAQDFAERR
                     GITFRERVERVVEIVKQVPEKVRGMFDGLRLPADGGQAQERAVPEREKAADPEEALRR
                     ARTRALVRHARAVDTIFEAQDRGGRASPEQVTELRDARKAFEEVRPYGPHDAEAAYKK
                     NPELASEAATGQVNRTIRALQLETELRTDPQIRADRFVESWQRLDRASQRQYQAGDIS
                     GYRATRSAMGDMAKSLERDPQLESILEGRKRDLGIGIDSGRSLGQELAFSHGIDLGRG
                     RGIGL"
BASE COUNT          453 a          937 c          948 g          574 t
ORIGIN      
        1 gcgcctggct ccacataggt ctcgacgagc tgatagccgc gtgaggcaca ataggcttcg
       61 ccctggcgct tctgatcggg aatggagaca tcgtgttcgg cctgccgtgc tgtcgagacg
      121 cgcaggtaaa gggcggcgcg cagggggatt gtggaggcgg gagcggtcat gcccggtgtc
      181 cttttccggt cgcgccgggc agtccatgtc cggtaagggg gaggatcagt tcgatgcggt
      241 cgcaaagcag cgtggggaca atctttcgcc cacggcgctc ggtgcggacg atgccgtagc
      301 tcgccagtct gtgaagtgtg cgcgacaggt tgggcttggc gcgccctgtc agttcgacca
      361 attcatcgag ggattcgggc gcgcgctccg cgatcaagcg cagcatcgtg cgattgtctg
      421 ccgtcaacaa gcgcgcaaaa gactccgttg atggaaacca cacggtgggg tcgccgggca
      481 tcgccttgcg gctttcgcca gcctctccga ttccgacttt cagaacgggc atcgtttgtc
      541 ctcgtgcggt tacgagaaca aatattatca tcacaagaca actgttcaag cgccaatcga
      601 gcatcccacg ctcagtcgcc ggggccagac agttcatcga acaaatcgtc gaaccattgc
      661 tcaaacacct cgatctcggt ttccgtaacc ggaacgggat ggggccaatc gtcggttacg
      721 gtccacgtgg acagatcatg tttgggcggc ctgccgggga atggccacgg atagcccaac
      781 agctcctcaa gctgttccga tgccgtcggc ggcctacttc ctttgcgacg cggtgctgtg
      841 cccgcccggt cagtcatcga agtcgaactc gcttctcgga cgccacgtca cgggattggc
      901 gacccagcga ttgatgtcag attcccgcca gccagcgcca ttgacgctga tcttgatccg
      961 agccgggaac gtgccttcgg tcatcttgcg gtagatggtg gaccgggaca gcccggtgcg
     1021 ggcgagaacg gttttcatgc ggacgatacg gtctgctacg agcatggctg cgctgcctcc
     1081 tgccgggtgt ttctgacgat tgcagagacc agacagagcg atgatttgtt gttgtgcaat
     1141 agatatttag aggtcgtagt agtgtggcgt ggaggcggcg gaaaatagga tagttctaca
     1201 gtccgatacc ccggcctctg ccgaggtcga tgccgtggct gaatgccagt tcctggccga
     1261 ggctgcggcc ggagtcgatc ccgataccga ggtctcgctt gcggccttcg aggatggatt
     1321 caagctgcgg atcgcgttca aggctcttcg ccatgtcgcc catcgctgac cgcgttgccc
     1381 tgtagccgga tatgtcaccc gcctggtact ggcgctggct ggcgcggtcc agcctctgcc
     1441 aactctccac gaagcgatcc gcacgaatct gcggatcagt gcgcagttcg gtttcgagct
     1501 ggagggcgcg gatggtgcga ttgacctggc ccgtggccgc ttccgaggca agctccggat
     1561 tcttcttgta ggcggcttcg gcgtcgtgcg ggccgtaggg ccgcacctcc tcgaaggctt
     1621 tgcgggcgtc ccgcaattcc gtcacctgct ccgggctggc cctgccgccc cgatcctgcg
     1681 cctcgaagat ggtatccacg gcgcgggcat ggcggacgag cgctctggtc cgggcgcggc
     1741 gcaaagcctc ctccgggtct gccgccttct ccctttccgg caccgcccgt tcctgcgcct
     1801 gcccgccatc ggcgggcagg cgcaggccat cgaacatgcc gcgcaccttc tcgggaactt
     1861 gcttgacgat ctcgaccacc cgctccaccc gctcgcggaa ggtgatcccg cgccgctcgg
     1921 caaagtcctg cgccggatcg gcgcgctcgt aatccgacgc catatccttg gcccggtcgc
     1981 gcgacagagt gcggatgagc cggtcctgat cggcgaagtc atcgcggcca tagtgcaggt
     2041 ccatgccgtc gcagtggcgc gacagggcaa catagctgcc gtgggcgtcc aggcccggcg
     2101 tcgccaggac atgggtgcgg tccaccgtca tgccctgcgc cttgtggatg gtcgcggcat
     2161 agccgtggtc gatgcggtcg taatccttga ggtcgaacga aatgctgcgc cggttgtcgg
     2221 tgcgcacgga catggatcgt gcgctgactt gctcgatggt cccgagcgtg ccgttcttca
     2281 cgccaagccc gcgctcgttt tgcaggaaca tgacgcgatc cccgctggcg aagctgcgct
     2341 cgccgcgttc gaccgtgata cgcacgtcgt cgcccagatc accggcttcc cgcagccgct
     2401 cgcgcgccgc ctcgttgagc gcgcgcacct cgtcattggt gtgggtgagg atgatgcggc
     2461 tcttgtcggg cgacgcttgc cgctcgcggt tccagccctc gatcagatcg tcgcgcgcct
     2521 gctcgcgtgt ttcggcggca tggaccatgc cgtgcgcgtc ataggcatgg atcgcttcgc
     2581 ccgtccggcc ggccgccagt tcgcgcgtgg cgtcgcgctg ccagtcctca cgctggtggc
     2641 gcacctcgcc gatctccgcg ccgccgtggc gctcgaagat cgagcggaag gccgcgcccg
     2701 cctcgatgga ttgcaactgt tgcggatcgc cgaccaagac tactttggcg cccgcgtcgg
     2761 cggcatggga cagcacgcgc tccaactggc gcgtgccgac catgccggcc tcgtcgatca
     2821 caagcacatc gcgggacgtg agtgcgtcgc ggccctgtcc ccaaccatgt tccatgctgg
     2881 cgatggtgcg cgacgcgatg cccgatccgc tt
//
LOCUS       DASWPU010000635         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_54427,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000635 DASWPU010000000
VERSION     DASWPU010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54427"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2105
                     /locus_tag="VGC27_13550"
     CDS             <1..2105
                     /locus_tag="VGC27_13550"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY0283632.1"
                     /translation="TATFSDIANAIAEERGFWLGDAFASGGSHGYDHKVMGITAKGAW
                     EAVKRHFRELGLDIQSRPFTCIGIGDMSGDVFGNAMLLSRCTKLLAAFDHRHIFIDPD
                     PDPAASWNERKRLFDLPRSSWADYDKTKISKGGGVFARSLKEIPLSDEIKALTGIARG
                     KAAPSELMKALLKADVDLLWLGGIGTYVKAAAQSNLDVGDRANDAVRVNGAELRAKVI
                     GEGANLGLTQLGRIEYALAGGRINTDAIDNSAGVDTSDHEVNLKILMGGPLRRGEISA
                     AERDAILAEMTGDVAALVLTDNYDQTLALSVAQSRGAADLDAHGRFMRDLEARGRLDR
                     AVEFLPDDGELRRRAQNGKGLTRPELAVLLAYAKLDLKAELIASDLPDDPHFLPELTG
                     YFPPAANAKFPGELERHRLKREIVTDVIANRVINLAGPVFVARMKEMSGAPAARIVRA
                     FAVADGAFGFGRLRARIDALDGRVDAAAQIGMYADIAEILRRLGLWFITNVPADANLG
                     ETVALYRRGMENLRGTFSGLISPYEAQDTEARIAALAAAGAPADVAEDVGVLPLTAAA
                     PEVALLAHNQALNLDLAAGAYFAMGAEVGLDRLRGLAGRITGGEHWDRLAIRRISDDL
                     FAGQRALTAAALAMLPADKAPGARAEGAEAVKLWAASHAGALARTKSFLAALERTGEL
                     SVAKLTLANSQIHELAEH"
     gene            2173..>2538
                     /locus_tag="VGC27_13555"
     CDS             2173..>2538
                     /locus_tag="VGC27_13555"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEY0283633.1"
                     /translation="MQSQLPDPAAVQLPAVIARNRSTVERGFWKKLLKVAGRIPFAED
                     LAAAYFCAVDPLSPGRVRGVLIAALAYFVIPTDAIPDFIAGFGFTDDAAVLATAIGLV
                     AGHIKPRHRQRARAALDLTN"
BASE COUNT          410 a          877 c          851 g          400 t
ORIGIN      
        1 gaaccgccac cttttccgac atcgccaacg ccatcgccga agagcgcggc ttctggctgg
       61 gcgacgcctt cgcctcgggc ggcagccacg gctacgacca caaggtaatg ggcatcacgg
      121 cgaagggcgc ttgggaagcg gtgaagcgcc atttccgcga actcggcctc gatatccaga
      181 gccggccctt cacctgtatc ggcataggcg acatgtcggg tgacgtgttc ggcaacgcca
      241 tgctgctctc ccgctgcaca aagcttctgg ccgccttcga ccaccgccac atctttatcg
      301 accccgatcc cgatcccgcc gcaagctgga acgaacgcaa gcggctgttc gacctgccgc
      361 gttcgagctg ggccgattac gacaaaacga agatttccaa gggcggcggc gttttcgccc
      421 gttcgctcaa ggaaatccct ctgtcggacg agatcaaggc gctgaccggc atcgcgcgcg
      481 gcaaggctgc gccgagcgaa ttgatgaaag cgctgctcaa ggcggatgtc gatctgctgt
      541 ggctgggcgg tatcggcacc tatgtgaagg cggccgcgca gagcaatctc gacgtcggcg
      601 accgcgccaa cgacgcggtg cgcgtgaacg gcgccgagtt gcgcgccaag gtgatcggcg
      661 aaggcgccaa tctcggcctc acccagctcg gccgcatcga atatgccctg gccggcggac
      721 ggatcaacac cgacgccatc gacaattcgg ccggcgtgga cacttccgac catgaggtga
      781 atctcaagat cctgatgggc gggccgctgc gccgcggaga aatctcggcg gcggagcgcg
      841 acgcgatcct ggccgagatg accggcgatg tcgccgcgct ggtgctgacg gacaattacg
      901 accagacgct ggcgctgtcg gtggcgcagt cgcgcggcgc cgcggacctc gacgcccatg
      961 gccgcttcat gcgcgatctg gaagcgcgcg gacggctcga ccgcgccgtg gagttcctgc
     1021 ccgacgacgg cgagttgcgc cgccgcgccc agaacggcaa aggcctgacg cggccggagc
     1081 tggcggtgct gctggcttac gccaagctcg acctcaaggc cgaattgatc gcaagcgatc
     1141 tgcccgacga cccgcatttc ctgccggagc tgaccggcta tttcccgccc gccgcgaacg
     1201 cgaagtttcc gggggagttg gaacgccatc gcctgaagcg cgagatcgtc accgacgtca
     1261 tcgccaaccg cgtgatcaat ctggcggggc cggtgttcgt ggcgcggatg aaagagatgt
     1321 cgggtgcgcc cgccgcgcgc atcgtccgcg ccttcgccgt cgccgacggc gccttcggct
     1381 tcggccggtt gagggcgcgc atcgacgcgc tggacgggcg ggtggatgcg gcggcgcaga
     1441 tcggcatgta cgccgatatc gcggaaatcc tgcgccggct cggcctgtgg ttcatcacca
     1501 acgttccggc cgacgccaat ctcggcgaga cggtggcgct ctaccgccgc ggcatggaaa
     1561 atctgcgcgg caccttctcg ggtctgatct cgccttacga ggcgcaggat accgaagcgc
     1621 gcatcgccgc gcttgcggcc gccggcgcgc cggccgacgt ggccgaggat gtcggtgtgc
     1681 tgccgctgac ggcggccgcg cccgaggttg cgctcctggc ccacaaccag gcgctcaatc
     1741 tcgatcttgc ggccggcgcc tattttgcga tgggggcgga agtgggcctc gaccggctgc
     1801 gcgggctggc cgggcgcatc accggcggcg aacattggga ccgtctggcg atccggcgca
     1861 tcagcgacga tctttttgcc ggccagcgcg cgctgacggc ggcggccctg gcgatgctgc
     1921 cagcggacaa agccccaggc gcgcgcgccg agggtgccga ggcggtgaag ctgtgggccg
     1981 caagccatgc cggcgcgctg gcgcgcacca agtccttcct cgctgcgctg gagcgcacgg
     2041 gcgagctgtc ggtggcaaaa ctgaccctgg ccaacagcca gatccacgaa ctggcggaac
     2101 actgagcgcc gttgcggcac ccgtgcgcaa ttccgccctt gcattgcccc aatccggcgc
     2161 gatgataggc ggatgcagag ccaactcccc gatccggcgg cggtccagct tcccgccgtc
     2221 atcgcgcgca accgcagcac ggtcgaacgc ggtttctgga aaaagctgct caaggtggcg
     2281 gggcgcattc ccttcgccga agaccttgcg gccgcttatt tctgcgccgt cgatccgctg
     2341 tcgccgggcc gcgtcagagg cgtgctgatc gcggcgctgg cctatttcgt gatccccacc
     2401 gacgccattc ccgatttcat cgccggcttc ggcttcaccg acgatgccgc cgtgctggcc
     2461 accgccatcg ggctggtggc cggtcacatc aagccgcgcc accgccagcg cgcccgcgcg
     2521 gcgctcgacc tcaccaac
//
LOCUS       DASWPU010000636         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_18302,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000636 DASWPU010000000
VERSION     DASWPU010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18302"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGC27_13560"
     CDS             <1..217
                     /locus_tag="VGC27_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283634.1"
                     /translation="RVCRANIEDYLRAARDQGFVCLPQDLSLEAATTQEIKWLRKNGA
                     TDDKLNQSSVEDAQWTAINALWPCKEE"
     gene            474..1169
                     /locus_tag="VGC27_13565"
     CDS             474..1169
                     /locus_tag="VGC27_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283635.1"
                     /translation="MTARLEVIVGPMFSGKTELLIAKLHRAQYARKRIRIIKPAHDTR
                     TQGFIASRAVNPDGTTEVTDKLSAIMVRGEKDFARLVGPADFDVLAVDEAQFFPLDEP
                     LKDSLGWFGRALRGLLRERRDSHLRIIVAGLDMDFAEQPFGPIPGLLAIADQVEKLTG
                     VCMVCGSDSGYISQRLIADEQQLVVGDAGEYQVRCRACYEPPAAARGLKFSADEWDAD
                     LPGTRQGRSQAAR"
     gene            complement(1223..>1852)
                     /locus_tag="VGC27_13570"
     CDS             complement(1223..>1852)
                     /locus_tag="VGC27_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283636.1"
                     /translation="TGPDLGPPLRYRPPPPPVASYANAVQVAEDATIDDAWPWVNLKA
                     LTLFPTDEGTLGTEVLSVSGSQMIILRFDLSQFKGKTADGWGILELTTQAVQWAPTNQ
                     EEFGYLRTVEINAGDPGWTRDTVTYDSLFRGKPELDVLNGQLIFDILPALQRGGKTLI
                     SVNPAVMARLLSGETKGLAIYPQGSLNAAFASSQAPGPNVRPTLYFNVK"
BASE COUNT          331 a          566 c          640 g          315 t
ORIGIN      
        1 acgtgtctgt cgcgcaaaca tcgaggatta tctgcgggcc gcgagggacc aggggttcgt
       61 ctgcctgccg caggatctgt cgctggaagc ggctacgacc caagagatca agtggctgcg
      121 caagaacggc gccaccgacg acaaactcaa ccagagcagt gtcgaagacg cgcaatggac
      181 tgccatcaac gccctgtggc cgtgcaagga agagtgaggc ggcggactcg cgcgtctcgt
      241 tgcctcgtca gcggaatgac gcggctgcta ggcagggggc ttggctgcgg aaggaagcgc
      301 aagacaatcc cgcttcgcca aggaagacct gctggaggcg caatggaccg cggccgacgc
      361 gctgtggtcg tgcagtaaga agtacccgcg gcgggttttc cacaggcaga ttttcgggac
      421 gcggatcgaa tcatgctcta gtaagggact gaaaacgaag tgaggtccgg cccatgacgg
      481 cgcgtctgga agtcatcgtc gggccgatgt tctcgggcaa gacggagctg ctgatcgcca
      541 agctccatcg cgcccagtac gccaggaagc gcatccgcat catcaagccg gcgcatgaca
      601 cgcgcaccca gggcttcatt gcctcgcggg cggtgaaccc cgacggcacc accgaagtca
      661 ccgacaagct ttcggccatc atggtgcggg gcgagaagga tttcgcgcgc ctggtcggcc
      721 cggccgattt cgacgtgctg gcggtggacg aggcgcagtt cttcccgctc gacgagccgc
      781 tgaaggattc gctcggctgg ttcggacggg cgctgcgcgg cctgttgcgc gagcgccgcg
      841 acagccatct gcgcatcatc gtcgccggat tggacatgga tttcgcggaa cagcccttcg
      901 gaccgatccc cggcctcttg gcgattgccg accaggtgga gaagctgacc ggcgtgtgca
      961 tggtgtgcgg ctccgacagc ggctacatct cgcagcggct gatcgcggac gagcagcagc
     1021 ttgtggtggg cgacgccggc gaatatcagg tgcgctgccg cgcctgctac gaaccgcccg
     1081 cggcggccag gggcttgaag ttttcggcag acgagtggga tgcggacctg ccgggaactc
     1141 ggcaaggaag aagccaagct gcccgttgat ctcatcgccg gcgaactcgc cggcctcatc
     1201 gtcaaaagcg cggaatcgcg ggctatttga cgttgaagta cagcgtcggg cggacgttcg
     1261 gccccggcgc ctggctcgag gcgaaggcgg cgttcaggct gccttgcgga tagatcgcca
     1321 ggcccttggt ctcgcccgac aacagccgcg ccatcacggc cggattgacg gaaatcagcg
     1381 tcttcccgcc gcgctgcaag gcgggcaaga tgtcgaatat caactgcccg ttgagcacgt
     1441 cgagttccgg cttgccccgg aacaggctgt cataggtgac ggtgtcgcgc gtccagcccg
     1501 gatcgccggc attgatctcg acggtcctga gatagccgaa ctcttcctga ttggtcggcg
     1561 cccattgcac cgcttgcgtc gtcagttcga ggatgcccca gccgtcggcc gtcttgcctt
     1621 tgaactgcga caggtcgaag cgcaggatga tcatctgcga accgctgacg gagagcactt
     1681 cggttcccag tgtgccttcg tcggtgggga acagggtcag ggctttgaga ttcacccagg
     1741 gccaggcgtc gtcgatggtg gcgtcctcgg cgacctgcac cgcgttggcg tagctggcga
     1801 ccggcggcgg cggcgggcgg tagcgcagcg gcgggcccag atcggggccc gt
//
LOCUS       DASWPU010000637         7966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_29793,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000637 DASWPU010000000
VERSION     DASWPU010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29793"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..1947)
                     /locus_tag="VGC27_13575"
     CDS             complement(316..1947)
                     /locus_tag="VGC27_13575"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF005737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HEY0283637.1"
                     /translation="MKVKTVATTPFDGQRPGTSGLRKKVAEFQQPRYLENFLQSIFDG
                     VGVGKTLVVGGDGRFHNREAIQTILRMAAANGVGRVLLGRGGLLSTPAISCVIRKYGA
                     SGGIVLSASHNPGGPGGDFGIKYNIANGGPAPERVTEAIYAESRKLDRYLIAGGEADF
                     DIDRLGASVFAGMKVEVIDPVADYAALMETLFDFGRISKLLTSGRFRMRFDAMHAITG
                     PYAHDIFEKRLGAPAGTVMNGTPLPDFGGGHPDPNLVHAHDLVAIMNGPGAPDFGAAS
                     DGDGDRNMILGRRFFVSPSDSLAVLAANARLVPGYAEGLAGVARSMPTSLAVDRVAAA
                     LKIPCFETPTGWKYFGNLLDAGRITLCGEESFGTGSNHVREKDGVWAVLFWLDLLAGR
                     QESVEAVVKAHWRAYGRNYYARHDYEGIDGKIATALIGDLRGKLAALEGGTFGGLKVV
                     RSDDFSYLDPVDGSVTEHQGVRVAFAGDARIVYRLSGTGTEGATLRVYLERYEPDPSR
                     HDCDVQEYLKDLASVAGQIAEIVPRTGRQSPSVAT"
     gene            complement(1944..4253)
                     /gene="hypF"
                     /locus_tag="VGC27_13580"
     CDS             complement(1944..4253)
                     /gene="hypF"
                     /locus_tag="VGC27_13580"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEY0283638.1"
                     /translation="MIRRVRIDVRGAVQGVGFRPHVYRSATRLGLAGWVENTSFGVRL
                     EVEGEAGAIAALRRAIEESPPPNAAIVSVEVEELEPRGEKSFVIGASASQGARVAQIL
                     PDLATCPDCLGELFDPKDRRYLYPFINCTHCGPRYSIVEDVPYDRARTSMRHFAMCPA
                     CRAEYDDPSNRRFHAEPNACPVCGPRLSLWNGDGAVLAQEHDALLDAAKAIRAGKIVA
                     AKGIGGFHLIADARNGEAVRRLRARKLRAEKPFAVMFPSLAVVERECAVSPEERALLE
                     SPARPIVLLRRKPGAIAASVAPGNPRLGVMLPYAPLHHLLLRELGFPVVATSGNLSDE
                     PIATGEAEALVRLKGVADLFLVHDRPIVRPIDDSVARIVCGRDQLLRRARGYAPAPIL
                     MDGLTPGILALGGHLKTTVAVTLEDGVLLSQHLGDLETVAAREAHARAVDDLVKLRGL
                     APRLVVRDLHPDYATSRMAEETGLPVTGVQHHLAHVASCMAEHGIAPPVLGVAWDGTG
                     YGTDGTIWGGEFLLVTESGWRRVAHLRCFRLPGGEAAMREPRRAAIGLLYEAFGDEAF
                     AMDDLPPLADFSPDERRVIQTMLQSGVNSPRTSSVGRLFDGFASLSGLRQSTSYEGQT
                     AAEFEWAADGIAAERPYDFPLIPGPAKDAPWIVDWKPALSGLLADRRKGAAPGVLSAA
                     FHDGLAAALVALARRIGEPRVILTGGCFQNARLTETSVSGLRAAGHEPIWHRHVPPND
                     GGIALGQAAWAAWLMSGRIASYEALGETA"
     gene            complement(4250..4513)
                     /locus_tag="VGC27_13585"
     CDS             complement(4250..4513)
                     /locus_tag="VGC27_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEY0283639.1"
                     /translation="MHEASLMKNLMRRLDEIAAAEKAKRITGVSVWLGAMSHMSPAHF
                     TEHFVESSTGTIADGATLDIAVSGDMNDANAQEILVRSVTVET"
     gene            complement(4574..5059)
                     /locus_tag="VGC27_13590"
     CDS             complement(4574..5059)
                     /locus_tag="VGC27_13590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEY0283640.1"
                     /translation="MPAPSRIIVLGIGNIERGDDGAGRAVVRLLRYMLSGVEIAEQDG
                     EAASLLDKLDGASTAYLVDACVSNAPPGTIHRIDAHLAPLPQASSDLSTHGFGLAAAI
                     ELGRALGRLPPRTIVYAIEAESFASGAPLSEAVATATVIVADRIRSEIDECFHPSTSS
                     G"
     gene            complement(5063..6352)
                     /locus_tag="VGC27_13595"
     CDS             complement(5063..6352)
                     /locus_tag="VGC27_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEY0283641.1"
                     /translation="MARKTIKVDYLARVEGESAFKVVVRDGVVKRAELRVFEPPRFYE
                     AFLRGRSYMEAPDITARICGICPVAYQMSAVHSMEKILGIRLGEPLRELRRLLYCGEW
                     IESHVLHIYMLHAPDFLGYDSAIHMAKDHGDIVKKGLELKKIGNDILNLVGGREIHPI
                     NVRVGGFYKTPAKRDLAPLAEKLKWARDAALDTVRLVARFDFPDNEREYEFVSLRHPS
                     EYPFNEGRVVSSKGIDIPVEDYDKHFREIHVEHSTALHSQIVGRGPYFVGPLARYSLN
                     FDRLSPLAQQAAREAGLGRSCANPFRSIVVRAVEVVYACDEALRVIESYVEPDRPYIA
                     AEPRAGVGCAATEAPRGLIYHHYELNADGSIKSARIVPPTSQNQPTIEKDLTSFVGGW
                     LDLPDIELQHRCEQAIRNYDPCISCSTHFLRLEMDRG"
     gene            complement(6342..7118)
                     /locus_tag="VGC27_13600"
     CDS             complement(6342..7118)
                     /locus_tag="VGC27_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283642.1"
                     /translation="MAGKKPRLAVWKFASCDGCQLSLLDCEDELLAVAGALDIAYFPE
                     ATRGEISGRYDVSLVEGSVTTAHDAERIKAVRARSRVLITIGACATAGGIQALRNFAD
                     VKAYIPAVYAHPEYIDTLATSTPIADHVKVDFELRGCPISKKQLVELISAFIAGRRPV
                     TPPHSVCMECKLKGNLCVMVAHGTPCLGPVTQAGCDALCPSYDRGCFGCYGPMETPNT
                     QALSKQLAALGMDGKAIERVYSTFNVNAKAFREEMKRHGS"
     gene            complement(7123..>7966)
                     /locus_tag="VGC27_13605"
     CDS             complement(7123..>7966)
                     /locus_tag="VGC27_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEY0283643.1"
                     /translation="ENTAAAADPMVPRVARVLRRRRDLKDVATLDLEMEDGGDLAFAP
                     GQFNMLTMFGVGEVAISISGDPAKPGGGLVHTIRDVSAVSGALMRLGPGSTVGLRGPF
                     GLGWPLADAKGRDVVVLAGGLGLAPLRPVIYSLLANRRDYGKIALLYGARGPGEILFR
                     AELEQWRRLLDIEVLVTVDHADADWHGSVGVVTTLIPRADFDPQHTLALVCGPEVLMR
                     FGSAVLRDVGVSDKSIYVSMERSMKCAVGLCGHCQVGPVFVCRDGPVFRSDQAAPLMA
                     VKEL"
BASE COUNT         1438 a         2588 c         2647 g         1293 t
ORIGIN      
        1 gccgccacga agaacactgg gccaaggcgg agaaggaaat tctgagcgag gccaaaccgg
       61 cgcgaaaaaa ggccaaagca gcgcccgagc ctaagccgct tgccaagcct tcggccaagc
      121 gaagcggcaa gcctaagaag aaatagggcg ggtaagagtg ttaagagcgt gatccgtccg
      181 aatgggaccg tgccgtacgc atgcttcgac aagctcagca tgaaggataa aacactgccc
      241 tcaccctggc cggtacgctg tcactaccgg ctgccgaagg gtgactggcg cgattcagcc
      301 agactcgatc acgttctacg tcgcaaccga ggggctttgg cgtccggttc tcggcacaat
      361 ctcggcgatc tgcccggcga cggaagcaag gtcctttaga tattcctgca catcgcaatc
      421 gtgacgggac gggtctggct cgtagcgctc caaatagacg cgcaaggttg cgccctcggt
      481 tcccgtgccg gacagccgat agacgatcct ggcgtcgccc gcgaacgcga cgcggacgcc
      541 ctgatgctcc gtcacgctgc cgtccaccgg atcgagataa gaaaagtcgt cgctacggac
      601 gaccttaagc ccgccgaacg tgccgccttc gagcgccgca agcttgccgc gcagatcgcc
      661 gatcagcgcg gtcgcgattt taccgtcgat gccttcataa tcgtggcgcg cataatagtt
      721 gcggccatag gcgcgccaat gagccttgac caccgcctcc accgattcct gccggccggc
      781 gagcagatcg agccagaaca gcaccgccca gacgccgtcc ttctcgcgca catggttcga
      841 gccggtgccg aaactttcct cgccgcacag cgtgatccgc ccggcatcca gaagattgcc
      901 gaaatatttc cagccggtcg gggtttcaaa acagggaatc ttcaacgcgg cggcgacgcg
      961 atccacggca aggctcgtcg gcatcgaacg cgcgaccccg gccaggcctt ccgcatagcc
     1021 cggaaccaga cgggcgttgg cggccagcac cgccagactg tcgctcggcg aaacgaagaa
     1081 gcgccggccc aggatcatgt tgcggtcgcc gtcgccgtcc gacgcggcgc cgaaatccgg
     1141 cgcgccgggc ccgttcatga tcgcgaccag gtcgtgggcg tggacgagat tgggatcggg
     1201 atggccgccg ccgaaatcgg gcaacggcgt gccgttcatc accgtgccgg cgggcgcgcc
     1261 caggcgcttc tcgaagatgt cgtgcgcgta agggccggtg atcgcgtgca tggcgtcgaa
     1321 gcgcatgcgg aaacgcccgg aggtcagcag cttcgagatg cggccgaaat cgaacagggt
     1381 ttccatcagc gccgcgtaat cggccaccgg atcgatgacc tccaccttca ttccggcgaa
     1441 gacggaggcg ccaagccggt cgatgtcgaa atccgcttcg ccgccggcga tgaggtaacg
     1501 gtcgagcttg cggctctcgg catagatcgc ctcggtcacc cgttcgggcg ccgggccgcc
     1561 attggcgata ttgtatttga tgccgaaatc gccgccgggc ccgcctggat tgtggctggc
     1621 cgaaagaacg atgccgcccg aggcgccgta tttgcggatg acgcaggata tcgccggtgt
     1681 ggacaacagg ccgccgcggc ccaaaagcac acggcccacg ccgttggccg cggccatgcg
     1741 caagatggtc tggatggctt cgcgattgtg aaagcggccg tcgccgccga cgacaagcgt
     1801 cttgcccacg cccacaccgt cgaagatcga ttggaggaaa ttctccagat aacggggctg
     1861 ttggaattcg gcgactttct tgcgcaggcc ggacgttccg gggcgctggc cgtcgaaagg
     1921 cgtggttgcg accgtcttca ccttcatgcg gtttctccaa gagcttcgta cgaggcaatt
     1981 ctgcccgaca tcagccaagc cgcccaggcc gcctggccga gcgctatgcc gccgtcgttg
     2041 ggcggcacat ggcgatgcca aataggttca tgcccggcgg cgcgcaagcc ggaaacggac
     2101 gtttcggtga ggcgcgcgtt ctgaaagcag ccgccggtga ggatcacgcg cggctcgccg
     2161 attcgccgcg ccaacgcgac cagcgccgca gctaaaccgt catggaacgc agccgacagg
     2221 acgccgggcg ccgcgccttt gcgccggtcc gccagcaggc cgctaagtgc tggtttccaa
     2281 tccacgatcc agggcgcgtc cttggccgga cctgggataa gaggaaagtc gtagggccgt
     2341 tccgccgcga taccgtcagc ggcccattcg aattccgccg cggtctggcc ttcatagctg
     2401 gtgctttggc gcaggccgct gagggacgcg aaaccgtcga acagacggcc gacgctggaa
     2461 gtgcgcggcg aattcacgcc gctctggagc atggtttgga tgaccctgcg ctcgtccggc
     2521 gaaaagtcgg ccagcggcgg aaggtcgtcc atggcaaagg cttcgtcgcc aaacgcttcg
     2581 tagagcaggc cgatggcagc gcggcgcggc tcgcgcatgg cggcttctcc acccggcagg
     2641 cggaaacagc gcaaatgcgc cacgcggcgc cagccagatt cggtcaccag caggaactcg
     2701 ccgccccaga tggtgccgtc ggtgccgtag ccggttccat cccaagcgac gcccagaacg
     2761 ggcggggcga ttccgtgttc ggccatgcaa gacgcgacat gggcgagatg atgctgtacg
     2821 ccggtgaccg gcagccccgt ctcttcggcc atgcggctgg tggcgtaatc ggggtgcagg
     2881 tcgcgcacca ccaggcgcgg ggccagtccg cgcagtttca ccaggtcgtc caccgcgcgt
     2941 gcgtgcgcct cgcgcgccgc gacggtttcc agatcgccca ggtgttgact caataacacg
     3001 ccgtcttcga gcgtgaccgc gacagtcgtt ttcaaatgcc caccgagcgc caggatgccg
     3061 ggagtaagcc catccatcag gatcggcgcg ggcgcatagc cgcgcgcgcg gcgcagcaac
     3121 tggtcgcgcc cgcagacgat ccgcgccacg gaatcgtcga tgggcctgac aatcggccgg
     3181 tcgtgaacca gaaaaagatc ggcgacgccc ttaagccgga ccagcgcttc ggcttcgccg
     3241 gtggcgatcg gctcgtcgga caggttgccg ctggtggcga cgaccggaaa gccaagttcg
     3301 cgcagcagaa gatgatgcag cggcgcgtaa ggcagcatga cgcccagccg gggattgccg
     3361 ggcgcaaccg acgcggcaat cgcgccgggc ttgcgccgca agaggacgat ggggcgcgcg
     3421 ggactttcga gcagcgcccg ctcttccggc gaaacagcgc attcgcgctc caccacggcg
     3481 agcgacggga acatcacggc aaagggcttt tccgcacgca gcttgcgggc gcgcaagcga
     3541 cggacggctt cgccgttgcg cgcatcggcg atcaggtgga agccgccgat gcctttggcg
     3601 gcgacgattt tccccgcgcg aattgccttg gcggcgtcga gcagcgcgtc atgctcctgc
     3661 gccagaaccg cgccatcgcc gttccacagg gacaagcgcg gcccgcaaac cggacaggcg
     3721 ttgggctcgg catggaagcg gcggttcgac gggtcgtcat attccgcgcg gcaggccggg
     3781 cacatcgcaa aatggcgcat cgaggttctg gcgcggtcgt aaggaacatc ttcgacgatg
     3841 ctgtagcgtg ggccgcaatg ggtgcagttg atgaagggat agagatagcg ccggtctttc
     3901 ggatcgaaca attcgccgag gcaatcgggg caggtcgcca gatcgggcag gatttgcgcg
     3961 acccgcgcgc cttgcgaggc gctggccccg atgacgaacg atttttcgcc gcgcggctcc
     4021 agctcctcga cttcgacgga gacgatggcg gcgttgggcg gcggcgattc ctcgatggcg
     4081 cggcgcaggg cggcaatggc gccggcctcg ccttcgactt ccagcctgac gccgaaactc
     4141 gtgttctcga cccaaccggc aaggccaagc ctggtcgcgc ttcggtagac gtgcgggcgg
     4201 aaccccaccc cctgcaccgc gccgcgaacg tcgatgcgaa cccgccggat cacgtctcca
     4261 ccgtgacgct ccgcaccagg atttcctggg cgttggcgtc gttcatgtcg cccgagaccg
     4321 cgatgtcgag cgtggcgccg tcggcgatgg tcccggtgga ggattccacg aaatgctcgg
     4381 tgaaatgggc gggagacatg tggctcatgg cgccgagcca gaccgatacg ccggtgatgc
     4441 gcttggcctt ctccgccgcc gcgatctcgt ccaggcggcg catcaggttc ttcatcagcg
     4501 atgcttcgtg catgtcctca ttccgcagcc ctcatgcttc gacaagctca gcatgaggga
     4561 ttagccaagc ccttcaccct gagcttgtcg aagggtggaa gcactcgtcg atctcggaac
     4621 gtatcctatc ggcgacgatc acggtagcgg tggcgacggc ctccgacagc ggggcgccgg
     4681 aggcgaagga ctccgcctcg atggcgtaga cgatggtgcg cggcggcaaa cggcccaggg
     4741 cgcggccgag ttcgatcgcc gccgccagac cgaagccatg cgtcgaaaga tcggaagagg
     4801 cctgcggcag cggggccaga tgggcgtcga tgcggtggat ggtgcccggc ggcgcgttcg
     4861 agacgcaggc gtcgacgaga taggccgtcg aagcgccgtc gagcttgtcc agcagcgaag
     4921 ccgcctcgcc gtcctgttcg gcgatttcca cgccggaaag catatagcgc agcagccgga
     4981 cgaccgcgcg ccccgccccg tcgtcgccgc gttcgatgtt gccgatgccg agcacgatga
     5041 tgcgggaagg cgcgggcatg ctctaacccc ggtccatttc cagccgcagg aaatgggtgg
     5101 agcaggaaat gcacggatcg taattgcgga tcgcctgctc gcagcgatgt tgcagttcga
     5161 tatccggcag atcgagccag ccgccgacga agctggtgag atccttttcg atggtgggct
     5221 ggttctggga agtcggcggc acgatgcgcg cgctcttgat gctgccgtcc gcattcagct
     5281 cgtaatggtg atagatcagc ccgcgcggcg cttcggtcgc ggcgcagccg acgccagcgc
     5341 gtggttcggc cgcgatatag ggccgatccg gctccacata ggactcgatg acgcgcaagg
     5401 cttcgtcgca ggcgtagacc acttcgacgg cgcgcaccac gatgctgcgg aacgggttgg
     5461 cgcagctcct gcccagcccc gcctcgcgcg ccgcttgctg cgccagcggc gacagtctgt
     5521 cgaaattcag gctgtaacgc gccagcggcc cgacgaaata ggggccgcgc ccgacgatct
     5581 gcgagtgcag cgcggtcgaa tgctcgacgt gaatttcccg gaaatgcttg tcgtaatcct
     5641 cgaccgggat atcgatgccc ttgctggaaa cgacgcggcc ctcgttgaag ggatattcgc
     5701 tcggatggcg cagcgagacg aattcatact cgcgttcgtt gtcggggaaa tcgaacctgg
     5761 ccaccaagcg aacggtgtcg agcgccgcgt cgcgcgccca tttcagcttc tcggccaaag
     5821 gcgccagatc gcgcttggcg ggcgtcttgt agaagccgcc gacccggacg ttgatggggt
     5881 ggatttcgcg cccgcccacc agattcagga tatcgttgcc gattttcttg agttccaggc
     5941 ccttcttcac gatgtcgccg tgatccttgg ccatgtggat cgcgctgtcg tagccgagga
     6001 aatccggcgc gtgcagcatg tagatgtgga gcacatggct ctcgatccat tcgccgcagt
     6061 aaagcagccg gcgcagctcg cgcagcggtt cgccgaggcg gatgccgagg atcttctcca
     6121 tcgaatgcac ggcgctcatc tggtaggcga ccgggcagat gccgcagatg cgcgccgtga
     6181 tgtcgggcgc ttccatgtag gagcggccgc gcaggaacgc ctcgtagaaa cgcggcggct
     6241 cgaagacgcg cagctccgcg cgcttcacga cgccgtcgcg caccaccacc ttgaacgcgc
     6301 tctcgccctc gacgcgggcg agataatcca ccttgatggt cttacgagcc atggcgcttc
     6361 atctcttcgc ggaacgcctt ggcgttcacg ttgaacgtgc tgtagacgcg ctcgatcgcc
     6421 ttcccgtcca taccgagcgc ggcaagctgt ttgctgaggg cctgggtgtt cggcgtttcc
     6481 atcggcccat agcagccgaa acagccgcga tcgtaggacg ggcacagcgc gtcgcaaccc
     6541 gcctgggtaa cggggccgag gcagggcgtg ccgtgcgcca ccatcacgca gagattgccc
     6601 ttgagcttgc attccatgca gacgctgtgc ggcggggtga cggggcggcg gccggcgata
     6661 aaggccgata tcagttcgac aagctgcttc ttgctgatcg ggcagccgcg cagttcgaaa
     6721 tcgaccttga cgtgatcggc gatcggcgtc gaagtcgcca gcgtgtcgat gtactccggg
     6781 tgggcataga ccgccgggat gtacgccttc acgtccgcga aattgcgcaa cgcctggatg
     6841 ccgcccgccg tcgcgcaggc gccgatggtg atcagcaccc gcgaacgcgc gcgcacggcc
     6901 ttgatgcgtt cggcgtcgtg ggcggtggtg acggagcctt cgaccaggga gacatcgtag
     6961 cggccgctga tctcgccccg cgtggcttcc gggaaatagg cgatgtcgag ggcgccggcg
     7021 accgcgagca attcgtcttc acaatcgagc aggctgagct ggcagccgtc gcaggaagcg
     7081 aacttccaaa ccgccagtct tggttttttc ccggccatcg cgtcaaagtt ccttcacggc
     7141 catcagcggg gccgcctgat cgctgcggaa gaccgggccg tcgcggcaga cgaagaccgg
     7201 gccgacctgg caatggccgc acagcccgac ggcgcatttc atgctgcgtt ccatcgacac
     7261 gtagatcgac ttgtcgctca cgcccacgtc gcgcagaacg gccgagccga aacgcatcaa
     7321 cacttccggc ccgcacacca gcgccagcgt gtgctgcgga tcgaagtcgg cccgtggaat
     7381 cagcgtggtg acgacgccga cggagccgtg ccaatccgcg tcggcgtgat cgaccgtcac
     7441 tagcacctcg atatcgagaa ggcggcgcca ctgttcgagt tccgcgcgga agaggatttc
     7501 gccggggccg cgcgcgccat aaagcaaggc gatcttgccg taatcgcgcc gattggcgag
     7561 cagggaatag atcacgggcc tgagcggcgc cagcccgagc ccgccggcca gcacgacgac
     7621 gtcgcgtcct ttcgcgtccg ccagcggcca gccgagaccg aacgggccgc gcaagccgac
     7681 agtgctgccg gggccgagcc gcataagcgc ccccgatacc gcgctgacgt cgcgaatggt
     7741 gtggaccaga ccgccgcccg gcttggccgg atcgccgctg atgctgatgg ccacctcgcc
     7801 gaccccgaac atggtcagca tgttgaactg gccgggcgcg aaggcgagat cgccgccgtc
     7861 ctccatctca aggtccagcg tggcgacatc cttgagatcg cgccggcgcc gcaacacgcg
     7921 cgccacccga gggaccatcg ggtccgcggc cgcggcggta ttttca
//
LOCUS       DASWPU010000638         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_91391,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000638 DASWPU010000000
VERSION     DASWPU010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91391"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VGC27_13610"
     CDS             complement(<1..821)
                     /locus_tag="VGC27_13610"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: protein motif:HMM:NF005715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY0283644.1"
                     /translation="MSIERIGVIGAGQMGNGIAHVLSLAGYDIVLEDIDPDQLGRAIA
                     TIKKNMQRQAGRGFIKEEEIEPALARIHPSQGLEGMRDRELVIEAAVEDEAVKRKLFQ
                     ELCPRLSDKAMIATNTSSISVTRLAASTDRPEHFIGLHFMNPVPVIQLVEVIRGIATN
                     DETFQAALDIVKKVGKTAAYAEDFPAFIVNRVLLPMINEAVYTLYEGVGNVDAIDTAM
                     LLGANHPMGPLQLADFIGLDTCLSVMQVLYEGLAYSKYRPCPLLVKYVEAGWLGR"
     gene            complement(853..1794)
                     /locus_tag="VGC27_13615"
     CDS             complement(853..1794)
                     /locus_tag="VGC27_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0283645.1"
                     /translation="MTALVIAEHDNKVLKDATAKTVTAATELGSGVHILVAGHDCGPV
                     ADAAAKLAGVEKVIVADDALYARMLAETMETLILSLAGNYGAILAPATSNGKNTLPRV
                     AAKLDTPQISEIVKVIAPDIFERPIYAGNAIQTVQAGAGVKVISVRTTAFKAAAATCS
                     AVIEKVAAAADPGSSKFLGEELSKSERPELTSAKIVISGGRGLQSAENFKLLEKIADK
                     LHAAVGATRAAVDAGFVPNDYQVGQTGKVVAPELYIAVGISGAIQHLAGMKDSKVIAA
                     INKDEEAPIFQIADIGLVADLFTAIPEMNEKLTEPGA"
     gene            complement(1791..2537)
                     /locus_tag="VGC27_13620"
     CDS             complement(1791..2537)
                     /locus_tag="VGC27_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY0283646.1"
                     /translation="MKVLVPVKRVIDYNVKVRVKTDQTGVDLANVKMSMNPFDEIAVE
                     EAVRLKEKGKASEVIAVSIGPVQASETIRTALAMGADRGILVKTEGEVEPLAVAKILK
                     AICEAERPVLVIAGKQAIDDDAGQTGQMLAALLGWPQATFAHTLDLGETSAKVEREID
                     GGLQTLEVKLPAVVTTDLRLNEPRYASLPNIMKAKKKPVEEKTPADYGVDPAPRLKVL
                     KVSEPPKRQGGVKVKTMSEFLAKLREAGVL"
     gene            complement(2806..>3021)
                     /locus_tag="VGC27_13625"
     CDS             complement(2806..>3021)
                     /locus_tag="VGC27_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY0283647.1"
                     /translation="GNGETGDDLYDKALAIVARERKATTSYIQRRLQIGYNRAARIIE
                     RMEEENVISKPNHKGLREVLLPEHEER"
BASE COUNT          485 a          920 c          956 g          660 t
ORIGIN      
        1 ttgcggccga gccagccggc ctcgacgtac ttcaccagca gtggacacgg gcggtacttg
       61 gagtaggcca ggccctcata gagtacctgc atgacggaca ggcaggtatc caggccgatg
      121 aagtcggcca gttgcagcgg ccccatcgga tggttggcgc ccagcagcat cgcggtgtcg
      181 atggcatcga cattgccgac gccctcatac agcgtataga ccgcctcgtt gatcatcggc
      241 agcaggacgc ggttgacgat gaaggcgggg aaatcctcgg cataggcggc ggtcttgccg
      301 accttcttga cgatgtcgag cgccgcctgg aaggtctcgt cattggtggc gatgccgcgg
      361 atcacttcca ccagctggat caccggcacc ggattcatga aatgcaggcc gatgaaatgc
      421 tccggccggt cggtcgaggc cgccagccgg gtcaccgaga tcgacgaggt attggtggcg
      481 atcatcgcct tgtcggaaag gcgcgggcaa agctcctgga agagcttgcg cttgacggct
      541 tcatcttcga cggccgcctc gatcaccagt tcgcggtcgc gcatgccttc caggccttgc
      601 gaagggtgga tgcgcgccag cgcgggttcg atttcctctt ccttgatgaa gccgcgcccc
      661 gcttggcgct gcatgttctt tttgatggtg gcgatggcgc ggcccaattg atcggggtcg
      721 atatcttcga gaacgatatc gtatccggca agcgacagga catgggcaat gccgttgccc
      781 atctggcccg cgccgatcac tccaattcgc tcgatactca tgcctggtgc cctagggttt
      841 gtcttctgtg gttcacgcgc ctggttccgt cagcttctcg ttcatttccg ggatcgcggt
      901 gaagagatcg gccaccaggc cgatgtcggc gatctggaaa atcggcgctt cttcgtcctt
      961 gttgatggcg gcgatgacct tggaatcctt catgcccgcc agatgctgga tggcgccgga
     1021 aatccccacc gcgatgtaga gttcgggggc cacgaccttg ccggtctgac ccacctggta
     1081 atcgttcgga acgaagccgg cgtcgaccgc ggcgcgggtg gcgcccacgg cggcatgcag
     1141 cttgtcggcg atcttctcca gcagcttgaa attctccgcc gattgcaaac cgcgtccgcc
     1201 ggagatgacg attttcgccg aggtgagttc gggccgttcg gacttcgaca attcctcgcc
     1261 gaggaatttc gaactgccgg ggtcggcggc cgcggcgacc ttctcgataa ccgccgagca
     1321 ggtcgctgcc gcggctttga aggcggtcgt gcgcacgctg atgaccttca cgcccgcgcc
     1381 cgcttgcacg gtctggatgg cgttgccggc atagatcggg cgctcgaaaa tgtcgggggc
     1441 gatcaccttc acgatttcgg aaatctgcgg ggtgtccagc ttggcggcga cgcgcggcag
     1501 ggtgtttttt ccgttgctcg tcgccggcgc caggatggcg ccgtagttgc cggcgaggga
     1561 gagaatgagc gtttccatcg tctcggcgag catcctggcg tagagcgcgt cgtcggcgac
     1621 gatcaccttc tccaccccgg ccagcttggc ggcggcgtcg gcgacggggc cgcaatcatg
     1681 gccagcgacc aggatatgca cgccggaacc gagttctgtg gctgcggtca ccgtcttggc
     1741 ggtggcatcc tttagtacct tgttatcgtg ctcggcaatg acaagcgcgg tcataaaacc
     1801 cccgcttcgc gcagcttggc gaggaactcg ctcatggttt tcactttgac gccaccctgg
     1861 cgcttgggag gttcgctgac cttgagcact ttcaggcgcg gggccggatc gacgccgtaa
     1921 tcggccgggg tcttttcttc gaccggcttc ttcttcgcct tcatgatgtt gggcagagag
     1981 gcgtagcgcg gctcgttgag gcgcagatcg gtcgtcacca ccgcgggcag cttgacttcc
     2041 agcgtctgca atccgccgtc gatctcgcgt tcgaccttgg cgctggtctc gcccaggtcc
     2101 agggtgtgcg cgaaggtggc ttgcggccag ccgagcaagg ccgccaacat ctgtccggtc
     2161 tggcccgcat cgtcgtcgat ggcctgcttg ccggcgatca ccaacaccgg tctctcggct
     2221 tcgcagatgg ctttgaggat cttggcgacg gccagcggct ctacctcacc ctcggttttc
     2281 accaggatgc cgcggtcggc acccatggcg agagcggtgc ggatggtttc ggaggcctgg
     2341 accgggccga tggagacggc gatcacttcg ctggctttgc ctttttcctt gaggcgcacc
     2401 gcttcctcga cggcgatttc gtcgaagggg ttcatcgaca ttttgacgtt cgccaaatcc
     2461 acgccggtct ggtccgtctt gacgcggact ttcacgttat aatcgatcac ccgcttcacg
     2521 ggcaccagga ccttcatggc gttagaaatt ccctgaatga acggccccat aacggccaat
     2581 gtgtctcgcg cccccgtatg ccaacggatt cgctgttttt agccgcccac tgtttgtgcg
     2641 gcgcaaaaac taggcccggc tccgccgccc tgtcaacgaa gcgccatcgg gccattcctt
     2701 agggggaagg gtggttactt agacatatta ttgcgtcgca gcatattgcc gcgtcattcc
     2761 accgtagatt gccgtttcag ggcgagcaga caggcaaaag tctacttatc gttcttcgtg
     2821 ttccggtagc agcacttcgc gcaacccttt atggttgggt ttggagatga cgttctcctc
     2881 ctccatccgc tcgatgatgc gggcggcgcg gttgtagccg atctgcaagc ggcgctggat
     2941 gtagctggtg gtggccttgc gttcgcgcgc cacgatggcc agcgccttgt cgtagagatc
     3001 gtcgccggtt tcgccgttgc c
//
LOCUS       DASWPU010000639         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_103230,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000639 DASWPU010000000
VERSION     DASWPU010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103230"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VGC27_13630"
     CDS             <1..1005
                     /locus_tag="VGC27_13630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HEY0283648.1"
                     /translation="ERAAALAPVAARYAVALSDTVAKLIDRTDPSDPIARQFIPSEAE
                     LIATPQERADPIGDDAHSPVPGIVHRHRDRVLFKLVHACPVYCRFCFRREMVGPGGET
                     ALSPQAFEAALAYIESHPEIWEVILTGGDPFILGARRAGEVGVRLASIPHVKVIRWHT
                     RVPVVDPAHIDDEFVASLIVPGAATIVALHANHSRELTAEARAACARLIDAGIPMLSQ
                     SVLLRGVNDDVETLEALMRAFVETRIKPYYLHQADLAPGTSHFRVPIEEGQALMRELR
                     ARVSGLCVPAYVLDIPGGYAKAPLNSSDVEQSGSGYRVRDHAGRWHRYPPEGFSDIPR
                     "
     gene            complement(986..2041)
                     /locus_tag="VGC27_13635"
     CDS             complement(986..2041)
                     /locus_tag="VGC27_13635"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY0283649.1"
                     /translation="MTYIKKPKLHHPGLAANKLGFTRRDYEGRISTLCAGCGHDSISA
                     AIVQACFELDIEPHRVAKLSGIGCSSKTPDYFLGQSHGFNAVHGRMPSVLTGANLANR
                     ELIYLGVSGDGDSASIGLGQFAHCLRRGVNMVYICENNGVYGLTKGQFSATADRGSKS
                     KTGSVNADEPIDLVSLAILLGATFVARSFSGDKKQLVPLIKAAISHRGSAVLDVISPC
                     VTFNNHAGSTKSFDFVRAHNEAVNKVDYVPAAKEITADYEEGATEIVRLHDGSLLKLA
                     KLRPDYDPSDRVGAMAHLQDRYEAGEIATGLLYLDPEPQNLHRHLGTIDTALNRLTQE
                     ELCPGAEALAAVNAEYR"
     gene            complement(2038..>2862)
                     /locus_tag="VGC27_13640"
     CDS             complement(2038..>2862)
                     /locus_tag="VGC27_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283650.1"
                     /translation="LLLAAYAGHGDTKHVLLFPEDPHEAFAFAADAFDLAERLQTPIF
                     VMMDLDIGMNDWLSKPFVWEKDRKLDRGKIMTAEELEAGKPFGRYADVDGDAIPYRTL
                     PGAHPALGAYFTRGSSHDRNARYTEEGSVYVDGMQRLLRKFETAKSLVPKPIIHKAKE
                     KAKYGVIYYGSTSAAMDEALDALERQGLYLDALRVRAFPFSHEVIDFVNTYETVFMLE
                     QNRDAQLQKLMVNECAIDPARFVSIPHYDGTPITARFITGAIADRMKLAKAKEAAE"
BASE COUNT          479 a          883 c          933 g          567 t
ORIGIN      
        1 gaacgcgccg ccgcgctcgc gcccgtcgcc gcgcgctatg ccgtcgcgct gtcggatacg
       61 gttgccaaac tgatcgaccg caccgatccg tccgatccca tcgcgcgcca gttcattcct
      121 tcggaagccg agttgatcgc aacaccgcag gagcgcgccg atcccatcgg cgatgacgcg
      181 cattcgccgg ttccgggaat cgtccatcgc caccgggacc gggtgctgtt caagctggtg
      241 catgcctgtc cggtctattg ccgcttctgc ttccgccgcg agatggtggg gccgggggga
      301 gaaaccgcgc tgtcgccgca agcgttcgag gccgcgctcg cctacatcga atcccatccg
      361 gaaatctggg aagtgatcct caccggcggc gatcccttca tcctcggcgc ccggcgcgcg
      421 ggcgaggtcg gcgtgcgcct ggcttcgatc ccgcatgtga aggtcattcg ctggcacacg
      481 cgtgtgccgg tcgtcgatcc ggcgcatatc gacgacgagt tcgtggcttc tttgatcgtg
      541 cccggtgccg ctaccattgt cgccctgcac gccaatcact cgcgcgaact gaccgccgag
      601 gcgcgcgcgg cctgtgcgcg cctgatcgat gcaggcatcc cgatgttgag ccagtccgtc
      661 cttctgcgcg gcgtgaatga cgatgtcgaa acgctggaag cgctgatgcg cgccttcgtg
      721 gaaacgcgca tcaaacccta ttacctgcac caagctgacc tcgcgcccgg cacctcgcat
      781 ttccgcgttc ccatcgaaga ggggcaggcg ttgatgcgcg aattgcgcgc tcgcgtttcc
      841 ggcctctgcg ttcccgccta tgtgctcgat attcccggcg gctacgccaa ggcgccgctc
      901 aactcaagcg atgtagagca gagcggaagc ggctaccgtg tgcgcgacca tgccggacgc
      961 tggcatcgct atccgccgga agggttcagc gatattccgc gttgaccgcg gcaagcgcct
     1021 cggcgccggg gcacagctct tcctgggtca accggttgag agccgtgtcg atcgtgccca
     1081 ggtgccggtg caggttctgc ggttcgggat cgaggtagag aaggccggtc gcgatctcgc
     1141 ccgcctcgta gcgatcctgc aaatgggcca ttgcgcccac gcggtcgctc gggtcgtaat
     1201 cggggcggag cttggccagc ttcagcagcg acccgtcgtg caggcgcacg atctccgtgg
     1261 cgccttcttc gtaatcggcg gtgatctctt tggccgcggg cacataatcg actttgttca
     1321 ctgcctcgtt gtgcgcgcgt acgaaatcga agctcttggt cgagccggca tgattgttga
     1381 aagtcacgca gggactgatg acatccagca ccgccgagcc gcgatgggaa atggccgctt
     1441 tgatcagggg gacgagctgc tttttgtcgc ccgaaaagct gcgcgccacg aaggtcgcgc
     1501 cgagcaggat cgccagggac accagatcga tcggctcgtc ggcattgacg ctgccggtct
     1561 tggattttga tcccctgtcg gcggtggccg agaactggcc tttggtcaga ccgtaaacgc
     1621 cgttgttctc gcagatgtag accatgttca cgccgcgacg cagacaatgg gcaaactggc
     1681 cgaggccgat ggaggcgctg tcgccgtcgc ccgagacgcc gaggtagatc agttcgcgat
     1741 tggcgaggtt ggcgccggtc agcacgctgg gcatgcgccc atgcacggcg ttgaagccgt
     1801 ggctttgtcc caggaaatag tccggcgtct tcgaagagca gccgatgccc gacagcttgg
     1861 cgacgcggtg cggctcgatg tcgagttcga agcaggcttg gacgatggcg gcgctgatgg
     1921 aatcgtggcc gcagccggcg cacagggttg aaatgcggcc ttcgtaatcg cggcgggtaa
     1981 agccgagctt gttcgccgcc agtcccggat gatgcagttt gggctttttg atgtaggtca
     2041 ttcggccgct tccttggctt tggcgagttt catgcggtcg gcgatggcgc cggtgataaa
     2101 ccgcgcggtg atcggcgtgc cgtcgtaatg agggatggaa acgaagcgcg ccggatcgat
     2161 ggcgcactcg ttgaccatca gcttctgcaa ctgggcgtcg cggttctgct caagcatgaa
     2221 cacggtttcg taggtgttca cgaaatcgat gacctcgtgc gagaacggaa aggcacgcac
     2281 gcgcagcgcg tcgagataaa gtccctgccg ttccagcgcg tccaacgctt cgtccattgc
     2341 ggcggaagtc gagccgtaat agatcacgcc gtattttgcc ttttccttcg ccttatgaat
     2401 gatcggcttg ggcaccaacg atttggcggt ctcgaatttc cgcagcaggc gctgcatccc
     2461 gtccacataa acgctgccct cttcggtgta gcgagcattg cggtcgtggc tcgatccgcg
     2521 ggtgaaatag gcgcccagcg cgggatgggc gcccggcagg gtgcgatagg gaatggcatc
     2581 gccgtccacg tcggcatagc ggccgaacgg tttgccggct tccagctcct ctgcggtcat
     2641 gatcttgccg cggtcgagct tgcggtcctt ctcccagacg aaaggcttgg acagccagtc
     2701 gttcatgccg atgtcgaggt ccatcatcac gaagatcggc gtttgcagcc gttccgccag
     2761 atcgaaggca tcggccgcga aggcaaaggc ttcgtgcggg tcttcgggga acagcaggac
     2821 atgcttggtg tcgccgtggc cggcataggc cgccagcaac ag
//
LOCUS       DASWPU010000640         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_73268,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000640 DASWPU010000000
VERSION     DASWPU010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73268"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /gene="guaA"
                     /locus_tag="VGC27_13645"
     CDS             complement(<1..1514)
                     /gene="guaA"
                     /locus_tag="VGC27_13645"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008108317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY0283651.1"
                     /translation="MTAPVLVIDFGSQVTQLIARRVREAGVYCEIVPFNLAEAALKEG
                     MPRAIILSGGPASVTEAHTPRAPQSVFEAGIPVLGICYGEMTMCEQLGGRVEAGHARE
                     FGRADIHIMKSSPLFKGLGEEGGREPVWMSHGDKITSIPPTFDVVATSENSPFAAIAD
                     ETRRFYGVQFHPEVMHTPRGAVLLRNFVVGIAGISPDWNMHAFRTRAIARIREQVGQG
                     RVICGLSGGVDSSVAAVLLHEAIGDQLTCIYVDTGLMRAGETEQVLSLFRGHYNIPLL
                     HADATDLFLSSLEGVGDPEKKRKIIGAAFIDVFEYEAAKAGGADFLAQGTLYPDVIES
                     VSATGGPSATIKSHHNVGGLPEHMKMKLVEPLRELFKDEVRALGRELGLPEEFVGRHP
                     FPGPGLAIRIPGAVTRDKLEVLRKADTIYLDEIRKAGLYDKIWQAFAVLLPVKTVGVM
                     GDDRTYDFVCALRAVTSSDGMTADSYPFQHAFLSRVATRIVNEVKGINRVVYDVT"
     gene            complement(1775..>2555)
                     /locus_tag="VGC27_13650"
     CDS             complement(1775..>2555)
                     /locus_tag="VGC27_13650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY0283652.1"
                     /translation="AEGFTVEPTRYAPHGLRLPSGEGLAKLSATALFADGAFEFQDEA
                     AQIAALLCAAKPGMRVLDLAAGAGGKSLALAAAMQNQGEIVAHDASPARLRPLALRAA
                     RAGATIIETCTDFSAPALAGEVASGASRRGLFDIVLIDSPCSGTGTWRRQPELRWRTT
                     PDGLKSLTGLQDGLLEQGAPFVTAGGRLVYATCSLLPCENEDRIERFLSRHGDFAVLP
                     ASAVWPETVGTAPPGLDRFFRATPLRTGTDGFFTAILQRFR"
BASE COUNT          460 a          846 c          842 g          407 t
ORIGIN      
        1 gtcacgtcgt agaccacgcg gttgatgccc ttgacttcgt tgacgatgcg cgtggccacg
       61 cgcgagagga aggcgtgctg gaagggatag gaatccgccg tcatcccgtc cgacgaggtc
      121 accgcgcgca aggcgcaaac gaaatcgtag gtgcggtcgt cgcccatcac gcccaccgtc
      181 ttgaccggca ggagcacggc gaaggcctgc cagatcttgt cgtaaagccc ggccttgcgg
      241 atttcgtcga gatagatcgt gtcggccttg cgcagaacct cgagcttgtc cctggtcacg
      301 gcgccgggga tgcggatcgc cagtcccggc cccgggaagg gatgacggcc gacgaattcc
      361 tcgggcaggc caagctcgcg gccgagcgcg cgcacttcat ccttgaacag ctcgcgcagc
      421 ggctccacca gcttcatctt catgtgttcg ggcaagccgc cgacattgtg atgcgacttg
      481 atggtggcgg aaggcccgcc ggtggccgag acgctctcga tcacgtcggg atagagcgtg
      541 ccctgggcga ggaaatccgc gccgcccgcc ttcgcggcct cgtattcgaa cacgtcgatg
      601 aaggccgcgc cgatgatctt gcgcttcttc tcgggatcgc cgacgccttc caggctggag
      661 aggaaaagat cggtggcgtc ggcatgcagc agcgggatgt tgtaatggcc gcgaaataaa
      721 gaaagcacct gttcggtctc gcccgcccgc atcaagccgg tatcgacgta aatgcaggtg
      781 agctgatcgc cgatggcttc gtgcagcagc accgcggcga ccgaggagtc cacgccacct
      841 gaaagcccgc agatcacgcg gccctggccg acctgctcgc ggatgcgcgc gatggcgcgg
      901 gtgcggaagg cgtgcatgtt ccagtcgggc gagatgccgg cgatgccgac gacgaaattg
      961 cgcagcagca cggcgccgcg cggggtatgc atgacctcgg gatggaactg cacgccataa
     1021 aaccggcgcg tttcgtcggc gatggcggcg aagggcgagt tctccgaagt cgccaccacg
     1081 tcgaaggtgg gcgggatcga ggtgatcttg tcgccgtggc tcatccacac cggctcgcgg
     1141 ccgccttcct cgccgagacc tttaaacagc ggcgacgatt tcatgatgtg gatgtcggca
     1201 cgtccgaact cgcgcgcatg ccccgcctcg acgcggccgc cgagctgctc gcacatcgtc
     1261 atctcgccgt agcagatgcc gagcaccggg atgcccgcct caaacacgct ttggggcgcg
     1321 cggggcgtgt gcgcttcggt gacgctggcc ggcccgccgg aaagaatgat cgcgcgcggc
     1381 atcccctcct tcagcgcggc ctccgccagg ttgaagggga cgatctcgca atagaccccc
     1441 gcctcgcgca cgcgccgcgc gatgagctgg gtcacttgag agccgaagtc gataaccaga
     1501 acgggagctg tcatggccgg atttaggcgg tcgcgcgccg ggttgtccag agaggtttcg
     1561 gactaaacag aatcattcct gtcattcccg gccgcccgcc tacgccaagg cttcggcggg
     1621 tcatgagtcg cacattgccc gccgtcgctt cagcgaaggc ggaagcgaag cgacggggag
     1681 ggaatccatg aacctgctca ggtttctgga ttcccttccc tcgcgctacg cgctcgccgg
     1741 gaatgacaat gggtatattc aaagcagacc cgccctagcg aaaccgctgc aaaatagccg
     1801 tgaagaaacc gtcggtcccg gttcgcaagg gcgtcgcccg gaagaagcgg tccaggccgg
     1861 gcggtgcggt ccccacggtt tccggccaga cggcggaggc gggcagaacg gcaaagtcgc
     1921 catggcgcga cagaaaacgc tcgatacggt cctcgttctc gcaaggcagg agcgaacagg
     1981 tcgcatagac cagccgcccg cctgcggtca cgaacggcgc cccctgttcc aagagaccgt
     2041 cctgcaagcc ggtcagtgac ttcagaccgt cgggcgtggt gcgccagcgc agttcgggct
     2101 gccgccgcca ggtgcccgtg ccgctgcagg gtgagtcgat caacacaatg tcgaagagcc
     2161 cccttcggct cgcaccgctc gccacttccc ccgccaaggc gggggcagaa aagtccgtgc
     2221 aggtttcgat gatcgtcgca ccggcgcgcg ccgcccgcag cgccagcggc cgcagccgtg
     2281 ccggactagc gtcatgggcg acgatctcgc cctggttttg catcgccgcc gccaaggcca
     2341 gggatttgcc gcccgcgccc gccgccaagt ccaggacgcg cataccgggc ttggccgcgc
     2401 acaacagcgc ggcgatctgc gccgcttcgt cctggaactc gaacgcccca tcggcgaaca
     2461 gtgccgtcgc gctcagcttc gccaagcctt cgcccgacgg aagtcgcaga ccgtgcggtg
     2521 catagcgcgt gggctcaacc gtaaagccct cggca
//
LOCUS       DASWPU010000641         4031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_79004,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000641 DASWPU010000000
VERSION     DASWPU010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79004"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VGC27_13655"
     CDS             <1..160
                     /locus_tag="VGC27_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEY0283653.1"
                     /translation="EAEMLRTPNFGRKSLNEIKAVLAEMGLHLGMEVPGWPPENIEDL
                     AKRYEDQY"
     gene            215..643
                     /gene="rplQ"
                     /locus_tag="VGC27_13660"
     CDS             215..643
                     /gene="rplQ"
                     /locus_tag="VGC27_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003850180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY0283654.1"
                     /translation="MRHRNQGRKLNVTSSHRIAMFANMAAALIKHEQITTTLPKAKEL
                     RPVVEKLVTLSRRGTADLHARRQALSQVRDITQVKKLFDVIGPRYAGRPGGYTRVLKA
                     GFRRGDNAPMAIIEFVDRDPEAKGKDSGPVVVSDEEKTEE"
     gene            794..2248
                     /locus_tag="VGC27_13665"
     CDS             794..2248
                     /locus_tag="VGC27_13665"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEY0283655.1"
                     /translation="MNRSSLLAAAGVVAFGFAVHLLVPSVTPAQPALAASPTLAAAPA
                     GKVPRAMSEVQLTFAPVVRHVAPAVVNVYSRSVVQAPAMNPLFNDPLFQQFFGSPQMR
                     QRVQQSLGSGVIVRADGLILTNNHVIQGGQDIVVALSDKREFKAKVVLADARTDLAVL
                     KIDTKGERLPTVEFGDSDRLQVGDLVLAIGDPFGVGQTVTMGIVSALARTEVSASNYQ
                     FFIQTDAAINPGNSGGALVTTDGKLAGINTAIFSRSGGNIGIGFAIPANLARRVVEGA
                     TSGGIKLPWIGADGQSVTGDIAASLGLSRPQGVLLKDIYPGGPAAQAGLKTGDVVLSI
                     DGTEVNDMQSLNYRVATRKPGDVVRAQVSSHGRASDVNIKLALPPGSGARDTATIGGR
                     NPLTGVQVESLSPAVALDLQMNLMAKGVVIRAVAAGTPAARYGFQPGDIVRAVNGQVI
                     TGVDQLKRVLDAAGGHWALAIDRGGQQMNLRVDG"
     gene            2356..3657
                     /locus_tag="VGC27_13670"
     CDS             2356..3657
                     /locus_tag="VGC27_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY0283656.1"
                     /translation="MPELPSDSVGNAPRPLADRLRPKTLAEVVGQDHLIGQEGPIGRM
                     VAENRPHSIMLWGPPGTGKTTIARLLSTAFKLHFEQLSAVFSGVADLKKSFDGARQRR
                     KIGQGTLLFVDEIHRFNRSQQDSFLPVVEDGTIILVGATTENPSFELNGPLLSRTQVL
                     VLRRLDDAALETLLQRAEAHYGEKLKLTADARDTLRAMADGDGRYLLNLVEEVAALKI
                     VGPLDPAALMTAVQKRAPLYDKSREEHYNLISALHKSVRGSDPDAALYWLARMLAGGE
                     RPLYILRRMVRASVEDIGLADPQALVQALAAKDMYDFLGSPEGEIAVVQCVLYLASAP
                     KSNAVYLAQGAAKRAAAEYGSLMPPAHILNAPTKLMKNLGYGKGYEYDHEAEEGFSGQ
                     NYFPDGMARQHFYQPKEMGFEREIQKRLDYWAKLRAKKSAE"
     gene            complement(3697..>4031)
                     /locus_tag="VGC27_13675"
     CDS             complement(3697..>4031)
                     /locus_tag="VGC27_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283657.1"
                     /translation="RLLERTQPEPFAAFHMEGHWDADGTRMIAAIRKAAGNPDIRIRK
                     MPWPLIRLLSPIVPLFRELSEMRYLWKLPIRMDNACLKAVLGEEPHTALDIAVRDTLT
                     GLGCLPAG"
BASE COUNT          735 a         1256 c         1347 g          693 t
ORIGIN      
        1 cgaagcggaa atgctccgca cgccgaactt cgggcgcaag tcgctgaacg agatcaaggc
       61 ggtgctcgcc gaaatgggcc tgcatctcgg catggaagtg ccgggctggc cgccggagaa
      121 catcgaagac ctcgccaaaa ggtatgagga tcagtattga gtttggtttc ccggctcgcg
      181 cttcgctggc cgggaatgac gaggttttaa ggaaatgcgc catcgcaatc agggccgtaa
      241 gctcaacgta acgtcgtctc accgcatcgc gatgttcgcg aacatggcgg cggcgctgat
      301 caagcacgag cagatcacca ccacgctgcc caaggcgaag gagcttcgcc ccgtggtcga
      361 gaagctggtg acgctgtcgc ggcgcggcac ggccgatctg catgcgcgcc gccaggcgct
      421 ctcgcaggtg cgcgacataa cgcaggtcaa gaagctgttc gacgtgatcg ggccgcgcta
      481 tgccggccgc cccggcggtt atacgcgcgt gctcaaggcc gggttccgcc gcggcgacaa
      541 cgcgccgatg gcgatcatcg agttcgtgga ccgcgatccg gaggccaagg gaaaggattc
      601 cgggccggtg gtcgtttctg acgaggaaaa aacggaggag taatcttccg tacagattcg
      661 tgcgcaacat tcggggcggc ccggctccga ccaaaggctt cgccgtggca agagagactc
      721 tcttggcgcg ccgaagcttt agcgaaggcg gctcggcgac gggtcgcctc tttgcgtctt
      781 ggggagtgag atcatgaatc gttcttcgct gttggctgcc gcgggcgtgg ttgcttttgg
      841 cttcgcggtt cacctgctgg ttccttccgt aacccccgcg cagccggcgc tggccgcgag
      901 tcctacgctt gccgccgcac ccgccggaaa agtcccccgc gcgatgagcg aggtgcagct
      961 caccttcgcg ccggtcgtgc ggcatgtggc gccggccgtg gtcaacgtct attcgcgcag
     1021 cgttgtccag gcgccggcga tgaatccgct gttcaacgac ccgctcttcc agcagttctt
     1081 cggaagcccg caaatgcgcc aacgcgtcca gcaatcgctg ggatcgggcg tgatcgtgcg
     1141 tgccgacggg ctgatcctga ccaacaacca cgtcatccag ggcgggcagg acatcgtcgt
     1201 ggcgctgtcc gacaagcgcg agttcaaggc caaggtggtt ctggccgacg cccgcaccga
     1261 tctcgcggtg ctgaaaatcg atacaaaagg cgaaagattg ccgacggtgg aattcggcga
     1321 cagcgaccgg ctgcaagtcg gcgatctggt gctcgccatc ggcgatccgt tcggcgtcgg
     1381 ccagaccgtg acgatgggca tcgtctcggc cctggcgcgc accgaagtca gcgcctcgaa
     1441 ctaccagttt ttcatccaga ccgacgccgc catcaatccg ggcaattcgg gtggggcgct
     1501 ggtgacgacg gacggcaagc tcgcgggcat caacacggcc atcttctcgc gttccggcgg
     1561 caatatcgga atcggttttg ccatccccgc caatctggcg cggcgcgtag tcgagggcgc
     1621 taccagcggc ggtatcaagc ttccctggat cggtgcggac ggccagtccg tcacgggcga
     1681 catcgccgcc agcctggggc tttcccggcc gcaaggcgtg ctgctgaaag atatctatcc
     1741 gggcggtccg gcggcgcagg cggggctgaa gaccggcgat gtggtgctat ccatcgacgg
     1801 caccgaagtg aacgacatgc agtcgctcaa ttatcgcgtc gctacccgta agcccggcga
     1861 cgtggtgcgg gcgcaggttt cgtcccacgg gcgtgcctcc gatgtgaaca tcaaactggc
     1921 cttgccgccg ggaagcgggg cgcgcgacac cgcgacgatc ggcgggcgca atccgctcac
     1981 cggcgtacag gtggagagcc tgtcgcccgc cgtggccctc gacctccaga tgaacctgat
     2041 ggccaagggg gtggtgatcc gcgcggttgc ggcgggcacg ccggcagcca gatacggctt
     2101 ccagccgggc gatatcgtcc gcgcggtcaa cgggcaggtc atcaccggcg ttgaccagtt
     2161 gaagcgcgtc ctcgacgcgg ccggcggcca ttgggcgcta gcgatcgacc gcggcggcca
     2221 gcaaatgaac ctgcgcgtgg acgggtagaa aggcgagtgg cgaataggga aaaagcaacc
     2281 attcgctatt cgctattcgc tattcgctat tcgcgctaat cttcggcgat gaccgacctc
     2341 tttgaagcag gcggattgcc cgagttgcct agcgacagcg taggcaacgc ccctcggccc
     2401 ttggccgacc ggctacgccc caagacgctg gccgaagtcg tgggccagga tcatctgatc
     2461 gggcaggaag gccccatcgg gcggatggtg gcggagaacc gtccgcattc gatcatgctg
     2521 tggggccccc cgggcaccgg caaaaccacc atcgcgcgcc ttctgtccac agcgttcaaa
     2581 ctgcatttcg agcaattgtc ggcggtgttt tccggcgtcg ccgatctgaa gaagtcgttc
     2641 gacggcgcgc gccagcggcg caagatcggg cagggcacgc ttttgtttgt ggacgaaatc
     2701 caccgcttca accgcagcca gcaggattcg ttcctgccgg tggtggaaga cggcaccatc
     2761 atcctggtcg gcgctaccac cgagaatccg tcgttcgagt tgaacggccc gctgctgtcg
     2821 cgcacgcagg tgctggtgct gcgccggctc gacgacgcgg cgctggaaac cctgttgcag
     2881 cgcgccgagg cgcattacgg cgagaagctg aagctgaccg ccgatgcgcg cgacacgctg
     2941 cgcgccatgg ccgacggcga cgggcgctat ctgctcaatc tggtggaaga ggtggcggcg
     3001 ctgaagatcg tggggccgct cgatcccgcg gcgctgatga cggcggtgca gaagcgcgcg
     3061 ccgctttacg acaaatcccg cgaggagcat tacaacctca tctcggcgct gcataagtcg
     3121 gtgcgcggct ccgaccccga cgcggcgctc tactggctgg cgcggatgct ggcgggcggc
     3181 gagcggccgc tctacatcct gcgccggatg gtgcgcgcca gcgtcgagga tatcggactg
     3241 gccgatcccc aggccctggt gcaggcgctg gcggcgaagg acatgtacga tttcctcggc
     3301 agcccggaag gcgagattgc cgtggttcaa tgcgtgcttt atctcgcttc agcgccgaaa
     3361 tcgaatgcgg tctacctggc gcaaggcgcg gcgaagcgcg cggccgccga gtatggctcg
     3421 ctgatgccgc cggcgcacat cctgaacgcg ccgaccaagc tgatgaagaa tctgggctac
     3481 ggcaaaggct acgaatacga ccacgaagcg gaagaaggct tttccggcca gaattatttt
     3541 cccgacggca tggcgcggca gcatttctat cagccgaagg aaatgggctt cgaacgcgaa
     3601 atccagaagc ggctggatta ttgggccaag ctgcgggcga agaagagcgc ggagtagacg
     3661 gtcagcacct gatgaggcga atgtgcttca tgcgtctcat ccggccggaa ggcacccaag
     3721 cccggtcaag gtatcgcgga cggcgatgtc cagcgcggta tggggttctt cgcccagcac
     3781 ggccttgagg caagcattgt ccatgcggat cggcagcttc cacagatagc gcatttccga
     3841 caattcccgg aacagcggca cgatgggcga caacagccgt atcagcggcc agggcatctt
     3901 gcgtatcctg atgtccggat tgccggcggc cttgcggatg gccgcgatca tccgcgtgcc
     3961 gtctgcatcc cagtggcctt ccatatggaa cgccgcgaaa ggctccggct gcgtccgttc
     4021 cagcaggcgc a
//
LOCUS       DASWPU010000642         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_48692,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000642 DASWPU010000000
VERSION     DASWPU010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48692"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VGC27_13680"
     CDS             <1..196
                     /locus_tag="VGC27_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY0283658.1"
                     /translation="QPICNPGKDSIAAVLHPAETKDLFFVADGSGGHVFAATAEEQAK
                     NVANWRKIEREQSASKPASR"
     gene            357..1244
                     /locus_tag="VGC27_13685"
     CDS             357..1244
                     /locus_tag="VGC27_13685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY0283659.1"
                     /translation="MAIVSMTGFAEAQGSREGAHWRWEVKSVNGRSLDLRLRLPPGFD
                     SIEAAARVLASSRFKRGNMQASLTFEAAASARGLRIDAAALASAVKIAKEVAAETGLR
                     PASIDGLLALKGVIVQDEGLEADAHEHAGRDAAVLESLAATFDALARARAGEGAKLAV
                     LLDAQIAEIARLTEAAGAAAAAQPAALRERLVQQIADLVAPGTVSPERLEQEIALLVV
                     KFDIREELDRLEAHVEEARRMLASGEAVGRKLDFLAQEFNREANTLCSKSSDIALTRI
                     GLDLKAVIDQFREQAMNVE"
     gene            1367..>1603
                     /locus_tag="VGC27_13690"
     CDS             1367..>1603
                     /locus_tag="VGC27_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY0283660.1"
                     /translation="MTVEIKRRGLMLVLSSPSGAGKTTLSRLLLDSDPNVAISVSATT
                     RPKRPGEVDGRDYFFIGDKKFQRRIEAGEFLEHAT"
BASE COUNT          288 a          517 c          544 g          254 t
ORIGIN      
        1 gcagccgatc tgcaatcccg gcaaggattc gatcgcggcg gttctccacc cggcggagac
       61 caaggacctg tttttcgtcg ccgacggcag cggcgggcat gtcttcgcgg ccaccgccga
      121 ggagcaggcc aagaacgtcg ccaactggcg caaaatcgaa agagagcaga gcgcttccaa
      181 gcccgcgtca cgttagctga tcatggggca atttgaattt ttcttcgtca tggccgctct
      241 tgagacagcc gtgacgaatg aggagaaggt tcaaaccaag ccacttccct ccggcgccac
      301 gggatgacag gcgggtattg ccggcggtaa gttctgcgtt cattcggagt cgccccatgg
      361 ccattgtcag catgaccggt tttgccgaag cgcaaggcag ccgcgaaggc gcgcactggc
      421 gctgggaggt caagagcgtc aacggccgca gcctcgacct gcgccttcgc ctgcctcccg
      481 gtttcgattc catcgaggcg gcggcgcggg tgctggcgtc gagccggttc aagcgcggca
      541 acatgcaggc ttcgctcacc ttcgaggcgg cggcgagcgc gcgcggattg cgcatcgacg
      601 cggcggcgct ggcctcggcc gtcaagatcg ccaaggaagt cgccgccgaa accggcctca
      661 ggcccgccag catcgacgga ctgctcgccc tcaagggcgt catcgttcag gatgaagggt
      721 tggaagcgga tgcgcacgaa catgccggcc gcgacgccgc cgttctcgaa agtctcgccg
      781 ccaccttcga cgctttggcg cgggcgcgcg ccggcgaggg cgccaaactg gccgttctgc
      841 tcgatgccca gatcgccgag atcgcgcggc tgaccgaggc cgccggagcc gccgccgccg
      901 cccagcccgc cgccttgcgc gagcggctgg tgcagcagat cgccgatctg gtcgcgcccg
      961 gaacggtgtc gcccgagcgc ctggagcagg agattgcgct gctggtggtg aagttcgaca
     1021 tccgcgagga actcgaccgg ctggaagcgc atgtcgagga ggcccgccgg atgctggcct
     1081 cgggcgaggc cgtcggccgc aagctcgact tcctggcgca ggagttcaac cgcgaggcca
     1141 acacgctgtg ctcgaaatcg tccgacatcg cgctgacgcg catcgggctg gacctcaagg
     1201 ccgtgatcga ccaattccgc gagcaggcga tgaacgtcga atgatccttc gagacgccct
     1261 ttcgcccgca ttctgagggg ccgtgcagcg gccgtctcga agggcgaggg cgaaagggct
     1321 cctcaggatg agggctggtg atggggagaa ggggcaataa tccggaatga ccgtcgaaat
     1381 caaacgccgc gggctgatgc tggtcctgtc ttcgccgtct ggcgcgggca agacgacgct
     1441 gtcgcggctg ctgctggact ccgatccgaa cgtcgccata agcgtctcgg ccacgacgcg
     1501 ccccaaacgt cccggcgagg tcgatgggcg cgattacttc ttcatcggcg acaagaaatt
     1561 ccagcgaagg atcgaggcgg gcgagttcct cgaacacgcc acg
//
LOCUS       DASWPU010000643         7700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_11210,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000643 DASWPU010000000
VERSION     DASWPU010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11210"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VGC27_13695"
     CDS             <1..807
                     /locus_tag="VGC27_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0283661.1"
                     /translation="ALGPRTALASIMWANNETGTIFPVAELADLARAAGALFHTDAVQ
                     AAGRLAIDVSATKIDMLSLSGHKLHGPKGIGVLYVRKGVQFSPLIRGGRQERARRGGT
                     ENVPAIVGLGKAAELAALRLAADSGRIKFLRDRFEDGVLKRIENCLINGERRNRLCNT
                     SNIAFDEAEAEAILHHLNGAQIAASAGAACAAGRMEPSHVLRAMNLPYTAVHGAIRFS
                     FSRDNTMEDVDRVLYVLPGIVAAARAVSPFGRERKAASPRPKRLAGMSTR"
     gene            804..1937
                     /gene="nifV"
                     /locus_tag="VGC27_13700"
     CDS             804..1937
                     /gene="nifV"
                     /locus_tag="VGC27_13700"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR02660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HEY0283662.1"
                     /translation="MKPPVIINDSTLRDGEQAPGVAFCREEKLAIARMLDEAGVDEIE
                     AGIPAMGQEEIETIAAIGKNLKHAVPIAWCRMLEADVDAAVKTGLSRVNLSVPVSDRQ
                     IRAKFRTGRADVLSRVTRVVAYARSMGLGVAVGGEDSSRADFAFVKRVIAAAEEAGAH
                     RYRFADTLGVMDPFAVFDTFKRLRAETGLELEFHGHDDLGLATANTLAAIRGGASHAS
                     VCVLGLGERAGNAALEEVVAGIDQILHRPTNVDLASLPALAELISSAARRPIPPGKSI
                     VGSAAFAHESGIHVAGLLRDPMTYEAFNPARFGRERHIVLGKHSGASAVRDALRSRGL
                     EADEGRLQCILDRVRRSASANKRPVSEIELLECFRATAPEAIQ"
     gene            2247..3047
                     /locus_tag="VGC27_13705"
     CDS             2247..3047
                     /locus_tag="VGC27_13705"
                     /inference="COORDINATES: protein motif:HMM:NF019005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--dinitrogen-reductase
                     ADP-D-ribosyltransferase"
                     /protein_id="HEY0283663.1"
                     /translation="MLPDRGHSTNLVSVPTGLLASTAFNAFPVPLHISSVRETNRTLF
                     NMLNDAQTAEDAADLFQSYMAAVFDLDDQDRNGRGPARFRASSYLRLLRGWGYEANSP
                     EGAVLKGWVESRFGLYPTFHKEPITRFPCPAWIGYVEEKMSSRFHSNAILAQLDLLYE
                     FCQWSLSRFHTVGRRHVTLYRGTNNYEEHLLVERLDKRRVVVRLNSLVSFTSERDVAE
                     SFGDIILEMDVPIVKILFFNALLPRHPLKGEAEFVVIGGDYEATATYY"
     gene            3072..3974
                     /gene="draG"
                     /locus_tag="VGC27_13710"
     CDS             3072..3974
                     /gene="draG"
                     /locus_tag="VGC27_13710"
                     /EC_number="3.2.2.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047407 - ADP-ribosyl-[dinitrogen
                     reductase] hydrolase activity [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyl-[dinitrogen reductase] hydrolase"
                     /protein_id="HEY0283664.1"
                     /translation="MLLDRALGAYLGLAIGDALGATVEFMTRREIESRYGVHNRLTGG
                     GWLKLKPGQVTDDTGMSLHLGRAIIRAGGWDPKAVCDEFVAWFRSRPVDVGNTCRRGI
                     SRYIADGSLSKPYNDGDAGNGACMRNLPVALATLGNDEEFERWTLDQCHVTHNHPLSD
                     DATLTVGRMVQTLLAGGGVKDCRRHTGALVEKHKAFHFEPYHGMSSAYIVDTIQTVLH
                     FYYRTDSFQTCLVEVVNQGGDADTTGAIAGMIAGATYGLAAIPDAWLRKLDAKVAAEI
                     RQQVPALLTLARGLDMSHPSTRLC"
     gene            complement(4140..4952)
                     /locus_tag="VGC27_13715"
     CDS             complement(4140..4952)
                     /locus_tag="VGC27_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEY0283665.1"
                     /translation="MTGALARIADGLATGRLIPYLGPETLLLGGTLSIPASSRALAEL
                     LSAKHTVPARARGNMWASAQFIETRRHRQTLSKLMADIFAPPTPPNPLHAWLAGLALP
                     LIVDAWYDDAMAAALSGSWGQIQAVTRNGKHEDIWYRPLRPDGATATAEEADSWTTIL
                     YKPHGAVKPNGEFLLSDSDYVEVLTEIDIQTPIPPLVQARRKSRGFVFLGCRFRDQME
                     RTFARQIMKRSAGPHFAVLAGETTRNEQRFLEEMAIERIDLPLGEAVSQLTR"
     gene            complement(5031..5228)
                     /locus_tag="VGC27_13720"
     CDS             complement(5031..5228)
                     /locus_tag="VGC27_13720"
                     /inference="COORDINATES: protein motif:HMM:NF024207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY0283666.1"
                     /translation="MAYKIIAEKCTGCGACEFECPNKAICEKGGVFLINEKKCTECAG
                     FFDTPQCAAACPIPKTCVLAF"
     gene            complement(5254..6741)
                     /gene="nifB"
                     /locus_tag="VGC27_13725"
     CDS             complement(5254..6741)
                     /gene="nifB"
                     /locus_tag="VGC27_13725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HEY0283667.1"
                     /translation="MVQHLVALPTIGDRIPGKSSCGGGCSASSAKGQSSCGTAAGQSD
                     LPPALWNKIKDHPCYSEDAHHYFARMHVAVAPACNIQCHYCNRKYDCSNESRPGVVSE
                     KLTPEEALRKVLAVAAEVPQLSVLGIAGPGDALANADKTFRTFELAAKALPHLKLCLS
                     TNGLAVPDHVDSIAAFNIEHVTMTINMVDPEVGARIYPWIFFKHKRWEGRDAARILHE
                     RQMEGLEALTGRGVLVKVNSVLIPGVNDTHLAEVNEAVRARGAFLHNIMPLISDPAHG
                     THYGLSGRRGPTPAELKAMQDRLGGGAKLMKHCRQCRADAVGLLGEDRGAEFMLDKLG
                     KAPVFDPGAREIYLTVVERERADRQRAKELAEMSLNRSNAEQPLLVAVATKGGGRINL
                     HFGHASEFQIYEVDSRGARFAGHRRADAYCQGGMGEEDALDGILKSLDGVSAVLCAKI
                     GAAPRQKLEAAGIAASEQYAFEYIEASAIAWYLARRQSAAMAQTA"
     gene            7138..>7700
                     /locus_tag="VGC27_13730"
     CDS             7138..>7700
                     /locus_tag="VGC27_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0283668.1"
                     /translation="MAALRQIAFYGKGGIGKSTTCQNTLAALAELGQKILIVGCDPKA
                     DSTRLILHAKAQNSVLSLAAEAGSVEDLEIEDVLKTGYRDIRCVESGGPEPGVGCAGR
                     GVITSINFLEENGAYDGVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALY
                     AANNISKGILKYASSGGVRLGGLICNSR"
BASE COUNT         1366 a         2478 c         2466 g         1390 t
ORIGIN      
        1 gcgctggggc cgaggaccgc gctcgcctcc atcatgtggg ccaacaacga gaccggcaca
       61 attttcccgg tcgcggagct tgcggacctc gcgcgcgcgg ccggcgcact gttccacact
      121 gacgccgttc aggccgccgg caggcttgcc atcgatgttt ccgcaaccaa aatcgacatg
      181 ctgtcactgt cgggccacaa attgcacggg ccaaaaggca tcggtgtact ctatgtgcgc
      241 aaaggcgttc agttcagccc gctgatccgc ggcggccgtc aggagcgcgc ccggcgcggc
      301 ggcacggaaa acgttccggc catcgtaggg ctcggcaagg cggcggaatt ggccgccctg
      361 cgcctcgccg cggatagcgg ccgcatcaaa ttcctgcgcg accgcttcga ggacggcgtg
      421 ctgaagcgca tcgagaattg tctcatcaac ggcgagcgca ggaaccggct ctgcaacacc
      481 tccaacatcg ccttcgacga ggcggaagcg gaggcgatct tgcatcatct caacggcgcg
      541 cagatcgccg cgtcggcggg cgccgcctgc gcggcagggc gcatggagcc ctcccatgtt
      601 ctccgggcga tgaaccttcc ctataccgcg gtgcatggcg cgatccgctt ttccttctcg
      661 cgcgacaata cgatggagga cgtggatcgg gttctttacg tgctgccggg catcgtcgcg
      721 gcggcgcgcg ccgtctcgcc ctttgggcgc gaacgcaagg cggcctcgcc gcggccgaag
      781 cgcctggccg ggatgtccac gcgatgaaac cgcccgtcat catcaacgac tccacgctca
      841 gggacggcga gcaggcgccc ggggtcgcct tttgccgcga agaaaaactc gcgattgccc
      901 gaatgctcga cgaggcgggg gtcgatgaaa tcgaagcggg cattccggcg atggggcaag
      961 aggagatcga aaccattgcc gccatcggca agaacctgaa acatgccgtg ccgatcgcct
     1021 ggtgccgcat gctcgaagcc gatgtcgatg cggcggtcaa aaccggtctg tcgcgggtga
     1081 acctgtcggt gccggtgtcg gaccggcaga tccgggcaaa gttccgcacc ggccgcgccg
     1141 atgtactgtc gcgcgtgacg cgggtggtgg cttatgcgcg cagcatggga cttggcgttg
     1201 cggtcggcgg cgaggattcg agccgggcgg atttcgcttt cgtcaaacgg gttatagccg
     1261 cggcggaaga ggccggcgcc catcgctacc gtttcgccga cacgctgggc gtgatggacc
     1321 cgttcgcggt tttcgatacg ttcaagcggc tgcgcgccga aaccggtctg gagcttgagt
     1381 tccacggcca cgacgatctc ggcctggcga ccgccaacac gctggcggcg atccgcggcg
     1441 gcgccagcca tgccagcgtt tgcgtgctcg gcctcggcga gcgcgccggc aacgccgcgc
     1501 tggaagaagt ggtcgccggg atcgatcaga ttctccaccg ccccaccaat gtcgatctgg
     1561 cgagcctgcc cgccttggcc gagttgatct cgtccgccgc ccgccggccg attccgccgg
     1621 gaaaatccat cgtcggctcg gccgccttcg cgcatgaatc cggcatccac gtcgcggggc
     1681 tgttgcgcga tccgatgacc tatgaagcgt tcaatcccgc gcgcttcggc cgcgagcgcc
     1741 atatcgtgct cggcaaacat tccggcgcct ccgccgtccg cgacgcgctt cgcagccgcg
     1801 gcctggaagc cgacgagggc aggctgcaat gcatcctcga ccgggtgcgc cgttcggcga
     1861 gcgcgaacaa gaggccggtc agcgaaatcg aactcttgga atgcttccgc gcgacggcgc
     1921 ctgaggcgat ccaatgaccg gagctgcgcc ttttcaaggt ccaccgggat gccgcggcgc
     1981 ccaagcccga acgcaccaag cctttcgttc cgtttgcggc gctgaccaag gccatcggca
     2041 aatgaggcac tagagcatga ttcaatctga ttaaatcgtt tccaatcacc cttcgacaag
     2101 ctcagggtga ggggagtggc cagaccttca tgctgagctt gtcgaagcat gtagttggcg
     2161 cgatccccat gctctagccg ggccgccggc aaactgtggt aaggtttgga ggggccagtg
     2221 tcgtggtttc tcggagatga aggtccatgc tgccggatcg cggccacagc accaacctcg
     2281 tcagtgttcc caccgggctg ctggcctcga ccgcgttcaa cgcctttccg gtgccgctgc
     2341 acatctccag cgtccgcgaa accaatcgca ctttgttcaa tatgctgaac gacgcgcaga
     2401 ccgccgagga tgcggccgac ctgttccagt cctatatggc ggcggtgttc gatctggacg
     2461 atcaggatcg caacgggcgc ggaccggcgc ggtttcgcgc ctcgagctat cttcgcctgc
     2521 tgcggggatg gggctacgaa gccaactcgc cggaaggcgc cgtgctcaag ggctgggtgg
     2581 aaagccgctt cggtctctat cccaccttcc acaaggagcc gataacccgg ttcccttgtc
     2641 cggcctggat cggctatgtc gaagaaaaga tgtcgagccg gttccactcc aacgccatcc
     2701 tggcccagct cgatctgctc tacgagttct gccagtggag cctctcgcgg tttcatacgg
     2761 tcggccgaag gcacgtcacc ttgtaccggg ggaccaacaa ttacgaggag catctgctgg
     2821 tcgagcgcct ggacaagcgc cgcgtcgtcg tgcggctgaa cagtctggta tccttcacct
     2881 cggagcgcga cgtcgccgag agtttcggcg acatcatcct tgagatggac gtgcccatcg
     2941 tgaagattct cttcttcaat gcgctgctgc cgcgccaccc tttgaaaggc gaagccgaat
     3001 tcgtcgtgat cggcggagac tatgaagcga ccgccacata ttactgatat ggtacgggca
     3061 tgaaggaagc gatgctgctc gatcgcgcgc tgggggccta tctcggcctg gccatcgggg
     3121 atgctctggg cgccaccgtc gaattcatga cccgccgcga gatcgagagt aggtacggcg
     3181 tgcacaaccg gctgaccggc ggcggctggc tcaagctcaa gcccggccaa gtgaccgacg
     3241 atacgggcat gtcgctccat ctgggccgcg ccatcatccg cgccggcggc tgggatccga
     3301 aggccgtttg cgacgagttc gtcgcctggt tccgctcgcg cccggtcgat gtcggaaata
     3361 cctgccggcg cggcatctcc cgctacatcg ccgacggctc gttgagcaaa ccttataacg
     3421 acggcgatgc cggcaacggg gcctgcatgc gcaacctgcc ggtggcgctg gcgacgctgg
     3481 gcaacgatga ggaattcgag cgctggacgc tggaccagtg tcacgtcacc cacaatcatc
     3541 ctttatccga cgacgccacc ctcaccgtcg ggcgcatggt ccagaccctg ctggcgggcg
     3601 gcggcgtcaa ggactgccgg cggcataccg gcgccctggt cgaaaagcac aaagccttcc
     3661 atttcgagcc ttatcacggc atgtcatcgg cttatatcgt cgataccatt cagacggtgc
     3721 tgcatttcta ttaccgcacc gacagttttc aaacctgcct ggtggaggtg gtcaaccaag
     3781 gcggcgatgc cgataccacc ggcgccatcg ccggcatgat cgccggcgca acctatgggc
     3841 ttgcggccat ccccgacgcc tggctgcgca agctggatgc aaaggtcgcg gcggaaatcc
     3901 gccagcaggt ccccgccctg ctcacattgg ccagaggatt agatatgtct cacccttcga
     3961 cccgcctatg ctaaagctcc ggcgggcgag cgataaagtt ctggcccgcc gtagccatgc
     4021 gaaggcgggc aagctcaggg tgagggactt ttacattccc ctcatgctgg gcctgtcgaa
     4081 gcatgagttt gtcgaagcat acggaggtcc atggcgtctt gataacaatc ggctcctggt
     4141 caccgcgtca gttgtgacac ggcttcgccg agcggcaggt cgatccgctc gatggccatc
     4201 tcttcgagaa agcgctgttc gttgcgcgtc gtttcgccgg ccaacacggc gaaatgaggg
     4261 ccggccgagc gtttcatgat ctggcgggcg aaggttcgtt ccatctggtc gcggaaccgg
     4321 cagcccagga agacgaagcc gcggctcttg cggcgcgcct ggaccagcgg cggaatgggc
     4381 gtctggatat cgatttcggt cagcacctcg acgtaatcgg aatcggagag cagaaattcg
     4441 ccgttcggct tcaccgcgcc gtgcggtttg tagagaatcg tggtccagct atccgcttcc
     4501 tcggccgtcg ccgtggcgcc gtccgggcgg agcgggcgat accagatgtc ctcatgcttg
     4561 ccgttgcggg tcactgcctg gatctgcccc cagcttccgc tcaacgcggc cgccatcgcg
     4621 tcgtcgtacc aggcatcgac gatcagcggc aaagccaagc cggccagcca ggcatgcagc
     4681 ggattgggcg gcgtaggcgg cgcaaaaata tcggccatca gcttggacaa ggtttgccga
     4741 tggcgcctgg tctcgatgaa ctgggcggaa gcccacatat tgccccgcgc ccgcgccggc
     4801 accgtgtgct tggccgacag aagctccgcc aaggcgcggc tcgaggccgg aatcgatagc
     4861 gttccgccga gcaggagcgt ttccggtccc agatagggga tcagcctgcc cgtcgccaga
     4921 ccgtccgcga tgcgggcgag ggcgccggtc aaggatcgat ccgccgggcc tcgacgccgg
     4981 tcggcccgcg ctcgacgaaa tcgccgtctc tcatgatccg cgccgcccgc tcaaaaggcc
     5041 agaacgcagg ttttgggtat cgggcaggcg gcggcgcatt gcggcgtgtc gaaaaagccg
     5101 gcgcattcgg tgcatttctt ttcgttgatc aggaagacgc cgcccttctc gcagatcgcc
     5161 ttgttggggc attcgaactc gcacgcgccg caaccggtgc atttctcggc aatgatttta
     5221 taggccatgt cggaacactt cctcagaggc tggtcatgcg gtttgcgcca tggccgcgga
     5281 ttgccggcgc gcgaggtacc aggcgatggc ggaggcttcg atatattcaa aggcgtattg
     5341 ctcgctcgcc gcgatgccgg cggcctccag cttctggcgc ggagcggcgc cgatcttggc
     5401 gcagagaacg gcggaaacgc cgtcgagact cttcaggatg ccgtccagcg cgtcctcctc
     5461 gcccatgccg ccctgacaat aggcatcggc acgccggtgg ccggcgaaac gggcgccgcg
     5521 cgaatcgacc tcgtaaatct gaaattcgct ggcgtggccg aagtgaaggt tgatgcgtcc
     5581 accgcccttg gtcgccaccg ccaccagcaa gggctgctcc gcgttcgatc tattcagcga
     5641 catctccgcc aactccttcg cgcgctgacg atcggcgcgc tcgcgttcga ccaccgtcag
     5701 gtagatttcg cgcgcgccgg ggtcgaagac gggcgccttg cccagcttgt ccagcatgaa
     5761 ttcggcgccg cgatcttcgc ccaacaggcc gaccgcgtcg gcccggcact ggcggcaatg
     5821 cttcatcagc ttggcgccgc cgcccaaacg atcctgcatc gccttcaatt ccgccggcgt
     5881 cgggccgcgc cggccgctga ggccgtaatg cgtgccgtgg gcgggatcgg aaatcagcgg
     5941 catgatgtta tgcagaaagg cgccgcgcgc ccgcaccgct tcgttgactt ccgccagatg
     6001 ggtgtcgttg acgccgggga tcagcacgga attcaccttg accagaacgc cccggccggt
     6061 cagcgcttca agcccctcca tctggcgctc gtgcagaata cgggcggcgt cgcggccctc
     6121 ccagcgtttg tgtttgaaga aaatccacgg ataaatccgc gcgccgacct caggatcgac
     6181 catgttgatg gtcatcgtca catgttcgat gttgaaggcg gcgatgctgt ccacatggtc
     6241 cggcacggcc aagccgttgg tcgagaggca cagcttgaga tgcggcagcg ccttggcggc
     6301 caattcgaag gtgcggaacg tcttgtccgc attggccagc gcgtcgcccg gcccggcaat
     6361 gcccaggacc gacaattgcg gcacctcggc ggccaccgcc aggactttgc gcagggcctc
     6421 ttcgggggtc agcttctcgc tgaccacgcc cggccggctt tcgttcgaac aatcgtattt
     6481 ccggttgcag tagtggcact ggatgttgca ggccggcgcc accgccacat gcatgcgggc
     6541 gaagtaatga tgggcatcct cgctgtagca gggatggtcc ttgatcttgt tccatagcgc
     6601 aggcggcagg tcgctttgtc cggccgccgt gccgcaagag gattgcccct tggcgctgga
     6661 ggcgctgcaa ccgccgccac acgagctctt gccgggaata cggtcgccga tggtcggcag
     6721 agcgacgaga tgttgaacca tgctgcactt ccgccggcga tgtctttcac acgcagcgaa
     6781 aattgcatgc tccgtgccac gcgggaaaaa ggcggaattg ctggcgtgag gcacgctgcg
     6841 tgacgtgtcg ggatggcgac aacccgacgg ccgcgttgca gatgcgacag cgagggcggg
     6901 ccggccgcgc ccgccatccg cgtccagaat tgtcggccat tgtcggcttc gcaacagacc
     6961 ggcaaagcct tgtcgcgatc cgctatgcgg ccatatcagc ctgaaaacaa agaagaattc
     7021 accgtccgcg acgagcgggg gaggttggca tggtcattgc gaaactctcc tcaagccggg
     7081 ccggccggtc cgtccgagtt cgcagttcgt gtggtgcatt cagcaaggag tatcccaatg
     7141 gcagcgttgc gacagatcgc attctacggc aagggcggga tcgggaagtc caccacctgc
     7201 cagaacacct tggccgcctt ggctgagttg ggccaaaaaa tactcatcgt cggctgcgat
     7261 cccaaggccg actcgacccg cttgatcctg catgccaagg cgcagaacag cgtgctgtcc
     7321 ctcgccgccg aggcgggcag cgtcgaggat ctggaaatcg aagacgtgtt gaaaaccggc
     7381 taccgcgaca tccgctgcgt cgaatcgggc gggccggagc cgggcgtcgg ctgcgccggc
     7441 cgcggcgtca tcacctcgat caacttcctg gaagagaacg gcgcctatga cggcgtcgat
     7501 tatgtgtcct acgacgtgct gggcgacgta gtgtgcggcg gtttcgccat gcccatccgc
     7561 gagaacaagg cccaggaaat ctacatcgtg atgtcgggcg agatgatggc gctctacgcc
     7621 gccaacaaca tctcgaaggg catcttgaaa tacgcctcca gcggcggcgt ccgcctcggc
     7681 gggctgatct gcaattcgcg
//
LOCUS       DASWPU010000644         6648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_11221,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000644 DASWPU010000000
VERSION     DASWPU010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11221"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VGC27_13735"
     CDS             <1..1108
                     /locus_tag="VGC27_13735"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF007186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEY0283669.1"
                     /translation="GMSVTALGALDPRSLIGTGRVGLLTATGTYDVERYPWAYEFWKR
                     QQQTHWMGEEVPLGNDIKDWSSDRVTPAERALLTQIFRFFTQSDVEVGDNYLKRYIPL
                     FQPLELQMMMAAFSNMETVHIDAYALLLKTLGMPKAEFEAFRDYAEMRAKADYMHSFG
                     VGTISDVARTLAMFGAFTEGMSLFASFAMLLNFPRFNKMSGMGQIVSWSVRDESLHCE
                     GIIRLFHEWNGETGAVTRAVRDDIIDVAKTMVALEERFVDLAFGIGGIEGMTAADINS
                     YVRYIADWRLTQLRLTPVFGYFEESASGYRQTKPHPLPWLVAILNGVEHANFFEQRAT
                     EYSKGASRGSWDGESGVWTLFDKREHKRSLEGKL"
     gene            1111..1659
                     /locus_tag="VGC27_13740"
     CDS             1111..1659
                     /locus_tag="VGC27_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283670.1"
                     /translation="MHTPDSITIRRAGWIALLTGATVLGSFIFACATPFAALATLAAI
                     HMNRRDAFMLMGVNWAANQIVGYTYLNYPQSWDSFAWGAAIGIAALAATGAAAGVESV
                     LRRAGWVVAAIGAFAIAFAAYEGLLYAATAILPSGSGAFSARVVLYVLSVNGLAFIAL
                     LALQRAGLALGFAVPRRGAAAA"
     regulatory      1808..2016
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2091..4127
                     /locus_tag="VGC27_13745"
     CDS             2091..4127
                     /locus_tag="VGC27_13745"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HEY0283671.1"
                     /translation="MQAQDGAVDELTVRVPEFVVKRRGDLVLFEAGKIRSAIERAGKV
                     TGEVDSTEAQLLAIQAIKVLSHLYRGAPPQVEQIQDVVEQVLISANHFKTARAYIVYR
                     EQHARLRHDRETIISVVSSINEYLDRKDWRVNANANQGYSLGGLILNTSGKVTANYWL
                     SHVYPPEIGRAHREGDFHIHDLDLLAGYCAGWSLRTFLNEGLNGVPGKVEAGPPKHLS
                     SAVGQIVNFLGTLQNEWAGAQAFSSFDTYMAPFVRADRLSFEDVRQGIQELVYNLNVP
                     SRWGTQTPFSNLTFDWTCPEDLREQTPLVGGKEMPFVYGDLQAEMDMINRAYIEVMTA
                     GDAKGRVFTFPIPTYNITADFPWESENAERLFEMTAKYGLPYFQNFLNSDLTPNMVRS
                     MCCRLQIDLRELLKRGNGLFGSAEQTGSVGVVTINCARLGHCHRGDAEALFARLDTLM
                     DLGRDSLEIKRKVIQRYMDDGLFPYTKRYLGTLRNHFSTLGVNGINEMIRNFTGDAEN
                     ITTEWGHRFALSFLDHIRARIVSFQEETGHLYNLEATPAEGTTYRFAREDRKRFPGIL
                     QAGTPDKPYYTNSSQLPVGFTDDPFEALERQDDLQRKYTGGTVLHLYMGERISSATAC
                     KKLVRRALERFHLPYITVTPTFSICPKHGYLEGEHEFCPKCDAEAIARKLRAAN"
     gene            4141..4314
                     /gene="nrdD"
                     /locus_tag="VGC27_13750"
     CDS             4141..4314
                     /gene="nrdD"
                     /locus_tag="VGC27_13750"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEY0283672.1"
                     /translation="MDRQDSQARELSDDERQRCEVWTRVMGYHRPVSSFNKGKQGEFA
                     ERVCFREPALRDA"
     gene            4307..4981
                     /locus_tag="VGC27_13755"
     CDS             4307..4981
                     /locus_tag="VGC27_13755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEY0283673.1"
                     /translation="MPELRIGGLSRLSSCDWPGELVATVFCQGCSWRCPYCHNPDLLP
                     ATGKSSIPWEEVRAFLATRRNLLDAVVFSGGEPLLQQALSEAMRDASAMGFKIGLHTG
                     GAVSSRFAEVLPLVDWVGFDVKAPFDDYARITGIDGSGRHARESLIALLASGVANEVR
                     TTVHPRLLGPGILDQMAHELAGLGVVCWVLQAFRGRSTTSLAPEPYEEDMLGTLKQCI
                     AQVEWR"
     gene            5421..5885
                     /locus_tag="VGC27_13760"
     CDS             5421..5885
                     /locus_tag="VGC27_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0283674.1"
                     /translation="MLSQKAKYALKALIALAESDEGELLQANEIAEKHNIPKKFLDLI
                     LLELRRHRLIDSRRGKKGGYLLLRPAETITIGDIVRAIDGPLAPIPCASVTAYRPCPD
                     CNDHKACMIRRIMREVRDAAAGILDNTSLAEAAQKKPSRKGERARRAARHAA"
     gene            complement(5952..6128)
                     /locus_tag="VGC27_13765"
     CDS             complement(5952..6128)
                     /locus_tag="VGC27_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283675.1"
                     /translation="MVLRSPRKNNGTASPILSDGIVARLMFVIVMSFLVGVVLLAPVH
                     AAEPARINTAAYSG"
     gene            complement(6151..>6648)
                     /gene="cysE"
                     /locus_tag="VGC27_13770"
     CDS             complement(6151..>6648)
                     /gene="cysE"
                     /locus_tag="VGC27_13770"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY0283676.1"
                     /translation="TAFLFFKGFHALESYRISHWLWRQKRQGLALLFQSSISQRFGVD
                     IHPAARLGRGIMIDHASGVVIGETAVVEDDASMLHGVTLGGTGKERGDRHPKVRAGVL
                     LSIGATVLGNVEVGDHARIGAGSVVLKPVPAHCTAVGVPARIVNCSNPQNPSYEMDHF
                     IGDAI"
BASE COUNT         1349 a         2059 c         2014 g         1226 t
ORIGIN      
        1 tggcatgtca gtgacggcgc tcggcgctct cgatccgcgc agcttgatcg gcacggggcg
       61 cgtaggcctt ctcaccgcga ccggcactta cgacgtggaa cgctatccct gggcctacga
      121 attctggaag cgccagcagc agacccactg gatgggcgaa gaggttccgc tcggcaacga
      181 catcaaggac tggtcctcgg accgggtgac gcccgccgag cgcgccctgc tgacgcagat
      241 ttttcgcttc ttcacccagt cggacgtgga ggtgggcgac aattacctga agcgctacat
      301 cccgctcttc cagccgctgg aactgcagat gatgatggcc gcgttctcca atatggaaac
      361 ggtccatatc gacgcctatg cgctgcttct aaagacgctt ggcatgccga aggcggaatt
      421 cgaggccttc cgcgactacg ccgaaatgcg cgccaaggcc gattacatgc acagcttcgg
      481 cgtcggcacg atctccgacg tggcgcgcac gctcgccatg ttcggcgcgt tcaccgaagg
      541 catgtcgctg tttgcaagtt tcgccatgct gttgaatttt ccgcgcttca acaagatgag
      601 cggcatgggc caaatcgtga gctggtcggt gcgcgacgaa agcctgcatt gcgagggcat
      661 catcagactc ttccacgaat ggaacggcga aacgggcgcg gtcacccggg ccgtgcgcga
      721 cgacatcatc gacgtggcaa agacgatggt cgcgttggaa gaacgcttcg tcgatctcgc
      781 cttcggcatc ggcggaattg aaggcatgac cgcagcggac atcaattcct atgtacgcta
      841 catcgccgat tggcggctga cccagctccg gttgacgccg gtgttcgggt atttcgagga
      901 aagcgcaagc ggttaccggc agacgaaacc ccatccgttg ccctggctgg tggccatcct
      961 caacggcgtc gagcacgcca acttcttcga gcagcgcgcc accgagtatt ccaaaggcgc
     1021 gagccgcgga agctgggacg gtgagagcgg cgtctggaca ctgttcgaca aacgggaaca
     1081 caagcgcagc ttggagggga agctttaggc atgcatacgc cggacagcat caccatcagg
     1141 cgcgccggtt ggatcgcgct gctcaccgga gcaacggttc tcggaagctt tatctttgcg
     1201 tgcgccaccc ccttcgctgc gcttgcaacg ctcgcagcaa tccacatgaa ccggcgcgat
     1261 gcctttatgc tcatgggcgt caattgggcc gccaaccaga tcgtcggcta tacttacctg
     1321 aactatccgc aaagctggga cagctttgcc tggggtgcag ccatcggcat tgcggcgctt
     1381 gcggcgacgg gcgcggcggc cggggtggaa tcggtactgc gccgggcggg ctgggttgtg
     1441 gccgcaattg gcgcattcgc tattgccttc gcggcgtacg aaggcttgct ttatgcggcc
     1501 accgcgatcc ttccgtccgg atctggggcc ttcagcgcga gggttgtcct ctatgtcctg
     1561 agcgtgaacg ggcttgcctt tatcgcgctc cttgcgctcc agcgtgccgg tcttgcgtta
     1621 gggttcgcag tacccaggcg cggtgcggca gccgcgtaag atgcctcgca ttgccgggat
     1681 ggcccgctgt tccggcggcc gcgaatcccc cgcaccgtct cgaaaccttg cccggcgccg
     1741 gccttcatca ttgtcacatg ttccctttcc cccaagtctg cgcttgactt cgcagcaagc
     1801 ggaaaactac gctatctggt gttacggttc tctggatgcg acaacatcta gggctaagag
     1861 ggaaaccggt aaggcttcgg ccaagccggt gctgcccccg caactgtgtg cggtgagtgg
     1921 ttgccaaccg gatgtcactg acgcttttgc gtcgggaaga ccggcaacga cgacgacccg
     1981 tgagccagga gacctgccgt aacgcgaaac gtccacgggc ggggtgtccg gctggccggc
     2041 tgcgggcgat tgctccgccg tcccgccctt ccgcctagaa agaggatcgt atgcaagcgc
     2101 aagacggcgc agttgatgaa ttgacggttc gcgtcccgga attcgtggtc aagcggcgcg
     2161 gcgacctggt cctgttcgaa gcggggaaaa tccgctccgc gatcgagcgg gcgggtaagg
     2221 tcaccggcga agtcgattcc acggaagccc agcttctcgc catccaggcc atcaaagtgc
     2281 tgagtcatct ctatcgcggc gcgccgccac aggtggagca gatccaggac gtcgtggaac
     2341 aagtgctgat cagcgccaac cacttcaaga cggcgcgcgc ctatatcgtc tatcgcgagc
     2401 agcatgcgcg gctcaggcac gaccgcgaaa ccatcatcag cgtggtctcg tccatcaacg
     2461 aatatctcga ccgcaaagac tggcgcgtga acgccaatgc caaccagggc tattcgctgg
     2521 gcgggctgat cctcaacacc tccggcaagg tgacggcaaa ctactggcta agccacgtct
     2581 acccgccgga gatcggccgc gcccaccgcg agggcgattt ccatatccac gatctcgatc
     2641 tgctcgccgg ttattgcgca ggctggtcgc ttcgcacgtt tctcaacgaa ggcctgaacg
     2701 gcgtaccggg aaaagtcgag gccggtccgc ccaaacacct gtccagcgcg gtcggccaga
     2761 tcgtgaattt cctgggtacg ctgcagaacg aatgggcggg agcgcaggcg ttcagttcct
     2821 tcgataccta tatggcgccg ttcgtgcgcg cggaccggct ctccttcgag gacgtgcgcc
     2881 aaggcatcca ggaactcgtc tacaacctca acgtcccgtc gcgctggggc acccagacgc
     2941 ccttcagcaa tctcaccttc gactggacct gccccgagga cttgcgcgag caaacgcccc
     3001 tggtcggcgg aaaagaaatg cccttcgtct acggcgattt gcaggccgaa atggacatga
     3061 tcaaccgcgc ctatatcgag gtgatgaccg cgggtgacgc caaggggcgc gtgttcacct
     3121 tcccgattcc gacctacaac atcacggcgg attttccctg ggagagcgaa aacgcagaac
     3181 gcctgttcga gatgacggcg aagtacgggc tgccctattt ccagaatttc ttgaactcgg
     3241 acctcacgcc caacatggtg cggtccatgt gctgccgcct gcaaatcgat ctgcgcgaac
     3301 tgctcaaacg cggcaacgga cttttcggca gcgccgagca aaccggatcg gtgggcgtgg
     3361 tcacgatcaa ctgcgcaagg ctcggccatt gtcatcgcgg cgacgccgag gcgttgttcg
     3421 cgcgcctgga tacgctgatg gacctcggac gcgacagcct agagatcaag cgcaaggtga
     3481 tccagcgcta catggacgac gggttgtttc cctataccaa gcggtatctc ggcacgctgc
     3541 gcaatcattt ctcgacgctg ggcgtcaacg gcatcaacga gatgatccgc aacttcacgg
     3601 gcgatgccga gaatatcacg accgagtggg ggcaccgctt cgccctgagc ttcctcgatc
     3661 acatccgggc gcgcatcgtg tcgttccagg aagaaaccgg ccatctctac aacctggaag
     3721 ccacccccgc cgaaggaacc acctatcgct tcgcgcgcga agaccgcaaa cgctttcccg
     3781 gcattctcca ggccggaaca ccggataaac cctattacac gaactcgagc cagcttccgg
     3841 tcggcttcac cgacgatccg ttcgaggcgc tggaacgcca ggatgacctg caacggaaat
     3901 acaccggcgg caccgtgttg cacctttaca tgggggaacg gatttcttcc gcgacggcat
     3961 gcaaaaaact tgtgcggcgg gcgctggagc gattccacct gccctacatc accgtaacac
     4021 cgaccttctc gatctgcccc aagcacggct atctcgaagg cgaacacgaa ttctgcccca
     4081 aatgcgacgc ggaggcgatt gcgcgcaaac tacgggcggc caattgaacg cgaggaacca
     4141 atggaccggc aagacagcca agcacgcgaa ctttcggacg atgagcgcca gcgctgcgaa
     4201 gtgtggacgc gcgtgatggg ctatcaccgc ccggtctcgt cgttcaacaa aggcaagcag
     4261 ggtgaattcg cggaacgcgt ctgtttccgc gaacccgcgt tacgcgatgc ctgagttgcg
     4321 gatcggcggt ctttcgcggc tgtccagttg cgactggccg ggagaacttg tcgcgaccgt
     4381 gttctgtcag ggttgttcat ggcgctgtcc ctattgccac aatccggacc tgttgcccgc
     4441 gaccggcaaa agctcgatcc cgtgggaaga ggttcgcgcg ttcctggcga cgcggcggaa
     4501 tctgctggat gctgtcgtgt tctcgggcgg cgaaccgctg ctgcaacagg ccttgagcga
     4561 ggccatgcgg gatgcctctg cgatgggatt caaaatcggg cttcacacag ggggggccgt
     4621 gtcgtcgcgg ttcgcggagg ttctgccgct tgtcgattgg gtgggattcg acgtgaaagc
     4681 cccgttcgac gattacgccc gcatcaccgg catcgacggc agcggcagac atgcccgcga
     4741 gagcctgatc gcccttctcg ccagcggtgt tgcgaatgaa gtgcggacga ccgtccaccc
     4801 gcgcctcctc ggccccggca ttctggacca gatggcgcac gaattggccg gtttgggcgt
     4861 ggtttgctgg gttttgcagg cattccgggg ccgcagcacg acatcacttg ccccggagcc
     4921 ctatgaagag gatatgctgg gcacgcttaa acagtgcatt gcgcaagtcg aatggcggtg
     4981 atcggacgat gacgggctat cccaatcgcc tttcgcatta cgtcgattgg tgcaaaaggg
     5041 ctggcgcagc gttgcgggcg gaggagacgg taagacaccg cccagcgcat agtcgggctt
     5101 gctgctgatc gccgctgctt cagcgcccct gcatcccacc tccgcaccag taaataacaa
     5161 caaaatcaaa gagttattga gaaatgaccg gaaaccgttc gccaacgcga acggaaggaa
     5221 aggcccggaa gcggagttca gtaccgacct aatcgaatgg ggatcggcct cgcaggctgg
     5281 gaccaaaacg gaaaaacgtg cccgggtaca attaccctat cgatgttata gggtcacata
     5341 tcagattctt tgaatataac cgtgctcgcc agggatgctc atgtcccggt gggcggaaga
     5401 taattgcgga gagaatttca gtgctatccc aaaaagccaa atacgcgctc aaggcgctca
     5461 tcgcgctcgc cgagagcgat gagggcgaac tgctgcaggc caacgagatt gccgagaagc
     5521 acaatattcc gaagaagttt ctggatttga ttctgctgga gcttcgccgg catcgcctga
     5581 tcgacagccg caggggaaag aagggcggct atctgctcct gcgccccgcc gagacgatca
     5641 cgatcggcga catcgttcgc gccatcgacg gtccgctcgc ccccattcca tgtgccagtg
     5701 tgacggccta tcggccttgt cccgattgca acgaccacaa agcctgcatg atccggcgca
     5761 tcatgcgcga agtgcgtgac gccgcggcag gcatactgga caacacctct ctggcagagg
     5821 ccgcgcagaa gaagccgtct cggaagggcg aacgagcgcg ccgggccgcg cgccacgcgg
     5881 cctgaggccg aaccaaactc cgcacacgat tacggcgagc aatcggaagg accggcgggc
     5941 gcgacgatcg cttatccgga gtatgcggcc gtgttgattc tcgccggctc tgccgcgtgg
     6001 acgggggcaa gaagcaccac gccaaccaga aatgacatga cgatcacgaa catcaggcgc
     6061 gcaacaattc cgtcgctgag aattggcgat gccgttccgt tgtttttgcg cgggcttctc
     6121 aacaccatcg gccactccct ccaaacgcga tcaaatggcg tccccgataa agtggtccat
     6181 ctcgtacgaa ggattctgcg ggtttgaaca attgacgatt ctcgcgggca cgccgaccgc
     6241 ggtgcaatgc gccggaacgg gcttcaagac gacggagccc gccccaatgc gcgcatggtc
     6301 tccgacttcg acgttcccga gcacggtcgc acctatggaa agcagcacgc ccgcgcgcac
     6361 ttttggatgc ctgtcacccc tctccttgcc cgtgccgccc aaggtcacgc cgtgcagcat
     6421 ggaagcgtcg tcttccacta cggccgtttc gccgataaca acaccgctgg cgtgatcgat
     6481 catgatgccg cgcccaagcc gggcggcggg atgaatatca acgccgaacc gttgcgagat
     6541 gctgctttgg aaaagcaaag ccaagccttg gcgcttctgc cgccacagcc agtgggaaat
     6601 acgatacgac tcgagcgcgt ggaaaccctt gaagaacagg aaggccgt
//
LOCUS       DASWPU010000645         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_79057,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000645 DASWPU010000000
VERSION     DASWPU010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79057"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VGC27_13775"
     CDS             complement(<1..1576)
                     /locus_tag="VGC27_13775"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0283677.1"
                     /translation="MPLIETIVAFCCRHARAVIAAGVLAAVASCLYTANNFAMNTNSV
                     TLTSADLPWRKEQARFDKLFPQRDNLILVVIDGATAERANGAANMLAAKLAENKMMFP
                     AVRRPDGGEFFERNGLLFLSEREVRETMQKLVTAQPFLGALAADPSLRGVMDSVDTAL
                     IGVARGDAKLADIDEPMAAFAGTLEKVQQGQASFLSWRTLFTGNTGGERLRAFVEVQP
                     KLDFDALSPGAVASKTIRQEALALGMTPDKGLRVRLTGPVPLADEEFASISDSAGLMS
                     LAMLLAVTFSLWLAVRSFRIIFCILATLITGLSLTMALGLLVVGVFNIISIAFVALFV
                     GLGVDFAIQFAVRYRSERYRNDDLVAALKRTGWRVGTPLALAAAATAAGFFSFIPTDY
                     VGVAELGLIAGMGMIVAFVLSVSLLPALIMLVKPAGEEMHVGFAFFAPVDEFMVTHRR
                     RILIAGAVVGAAALATVPFLKFDFNPLNLRNRHVESMATVFDLMKDPDTSPNTIDVMA
                     PSLADTDKLAAKLQQNP"
     gene            complement(1610..>2127)
                     /locus_tag="VGC27_13780"
     CDS             complement(1610..>2127)
                     /locus_tag="VGC27_13780"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY0283678.1"
                     /translation="GIHNFLSNLTLPVTVANNMLQGEATQAGKMICRFGINTTVGILG
                     VLDPASGMGYPEDEQDFGRTLAVYGVPSGPYLVLPLLGSTMPRDLAGKLLVDHYFNPL
                     GYVTYDGKLYVSLTENLLKLVDQGSRKINALRDVERTSIDYYAAMRSIHMQRRDNGTG
                     NLNVAPGVPTP"
BASE COUNT          399 a          642 c          735 g          351 t
ORIGIN      
        1 gcggattttg ctgcagtttg gcggcgagct tgtcggtgtc ggcgagcgag ggcgccatga
       61 cgtcgatggt gttgggcgag gtatcgggat ccttcatcag gtcgaacacg gtcgccatcg
      121 attcgacgtg acggtttctc agattcaacg gattgaagtc gaacttgagg aacgggaccg
      181 tggccagcgc ggccgcgccc acgaccgcgc cggcgatcag gatgcggcgg cggtgcgtca
      241 ccatgaattc gtcgacgggc gcgaaaaagg cgaagccgac atgcatttct tcgccggcgg
      301 gcttgaccag catgatcagc gccggcagca gcgaaacgct gagaacgaag gcgacgatca
      361 tgcccatgcc ggcaatgagg ccaagctcgg cgacgccgac ataatcggtg ggaatgaacg
      421 agaagaaccc cgccgccgtc gccgccgccg ccagcgccag aggcgtaccg acgcgccagc
      481 cggtccgttt caaggcggcg accaaatcgt cgttgcggta gcgctccgag cggtagcgca
      541 ccgcgaattg gatggcgaaa tcgacaccaa ggccgacgaa cagggcgacg aaggcgatcg
      601 agatgatgtt gaacacgccc accaccagca ggcccagggc catggtcagc gacagacccg
      661 tgatcagcgt ggccaggatg cagaagatga tgcggaacga acgcaccgcc agccagagcg
      721 aaaaggtgac ggcgagcagc atcgcgaggc tcatcagccc ggcgctgtcg gagatgctgg
      781 cgaactcctc gtccgccagg ggaaccggcc cggtcaggcg tacgcgcaag cccttgtccg
      841 gcgtcatccc gagcgcgagc gcttcctgac ggatcgtctt gctggcgacg gcgcccggcg
      901 acagggcgtc gaaatcgagc ttgggctgca cctcgacgaa tgcgcgcaac cgttcaccgc
      961 cggtgttgcc ggtaaacagc gtccgccacg acaggaagga agcttgtcct tgctggacct
     1021 tctccagcgt gccggcaaag gcggccatcg gttcgtcgat gtcggccagc ttggcgtcgc
     1081 cccgggcgac gccgatcagc gccgtgtcga cgctgtccat caccccgcgc agggaaggat
     1141 cggcggcaag cgcgcccagg aacggctgcg cggtgacgag cttctgcatc gtctcgcgta
     1201 cttcccgttc ggagaggaac aacaggccgt tgcgctcgaa gaactctccg ccgtcgggcc
     1261 ggcgcaccgc cgggaacatc atcttgtttt cggcgagctt ggcggccagc atgttggccg
     1321 cgccgttggc ccgttcggcc gtcgccccgt cgatcaccac caggatgagg ttgtcgcgct
     1381 gggggaacag cttgtcgaag cgcgcctgtt ctttgcgcca gggaagatcg gccgaggtca
     1441 gcgtcacgct gttggtgttc atggcgaaat tgttcgccgt gtagaggcag gacgcgacgg
     1501 ccgcgagaac gcccgccgcg atgacggcgc gcgcgtggcg gcagcagaac gcgacgatgg
     1561 tttcgatgag cggcatcaga acgccttggg cccgaatggc ggcaggatac tatggtgtcg
     1621 gaacgccggg cgcgacattc aagttgccgg tcccgttgtc gcgcctttgc atgtggatgc
     1681 tgcgcatggc ggcgtaatag tcaatggagg tccgctcgac gtcgcgcagc gcgttgatct
     1741 tgcgcgaccc ctgatcgacc agcttaagga ggttttcggt gagggagacg tagagcttgc
     1801 cgtcgtaggt gacgtagccc aaggggttga agtagtggtc gacaagaagc ttgccggcga
     1861 ggtcgcgggg catggtcgag ccgagcagag gcaacaccag atacggtccg ctcggcacgc
     1921 cgtaaaccgc cagggtccgg ccgaagtcct gttcgtcctc gggatagccc atcccgctcg
     1981 cggggtccaa gacgcccaga atgccgaccg tggtgttgat gccgaagcgg cagatcatct
     2041 tgccggcctg ggttgcctcg ccctgcagca tattgttggc gaccgtgacc ggcagcgtca
     2101 ggttcgagag gaaattatgg atgcctt
//
LOCUS       DASWPU010000646         4852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_54590,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000646 DASWPU010000000
VERSION     DASWPU010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54590"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="VGC27_13785"
     CDS             <1..1679
                     /locus_tag="VGC27_13785"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY0283679.1"
                     /translation="YVPKRAERLAWLTAFTGSAGVAVVLADKAAIFVDGRYTLQVRKQ
                     TDTSLFEPRDLVTEGPAAWIEANAKGAKLGYDPWLHTAAVVEGLKASAKKAGATLVAC
                     DTNPIDAVWSDQPDAPAAKAVPHGLPLAGESAEAKRIRIAEEVKKKGADATVLTMPDS
                     ICWLLNIRGADVPHTPFALAFSILNEDGSTDLFMDERKSSPELALHLGNAVRLRAPNE
                     FAPALDALSGKTVAADPGTAAAAIFDRLGKAGAKILHLSDPCQLAKACKNSIEVEGAR
                     KAHIRDGAALTKFLCWFAREAPKGKLDEIGASAKLEEFRKATGALSDLSFDSISAASE
                     HAAIPHYRVTRSSNLPIRQGEIYLIDSGAQYPDGTTDVTRTVIAGTPSAEMKDRFTRV
                     LKGHIALATAKFPEGTTGAALDSFARRPLWDAGLDYDHGTGHGVGSFLSVHEGPQSIS
                     KRQVTQALKPGMIVSNEPGYYKAGEYGIRIENLIVVRALGLAPGGDRQMMEFETITLA
                     PIDLNLVEVSLLTAEERAWLNDYHARVREKLTRLVDDETRAWLEQATRGI"
     gene            1692..1997
                     /locus_tag="VGC27_13790"
     CDS             1692..1997
                     /locus_tag="VGC27_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283680.1"
                     /translation="MRTYKVKRGARDKFIEVLRTTGFAELKRIGVKCAGPFASIEDET
                     TIFWMRGFPDEASRGPMSNQFYGGDAWKNELADAFMPNLEKYDVVAVEMPESAVQWV"
     gene            complement(2232..2651)
                     /locus_tag="VGC27_13795"
     CDS             complement(2232..2651)
                     /locus_tag="VGC27_13795"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0283681.1"
                     /translation="MSLLVVDASVALKWFLAEEGSGEADRLQAGHELIAPDIIVAELV
                     NALWKAFQKKLAPVDQLERSVDALAEPFSKLVPSVLLAAQAWHVATTLHYPAYDAFYI
                     ALAEREGCELVTADRRLLQATAGTDFEKFVRPLESRA"
     gene            complement(2648..2884)
                     /locus_tag="VGC27_13800"
     CDS             complement(2648..2884)
                     /locus_tag="VGC27_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283682.1"
                     /translation="MGQIIIRNLDDAVLAQLKKRAAEQGLSLEESLRRQLAEIARPSK
                     EEVLAKLRSIREMSPLRTAPPFAEDLIREGRDER"
     gene            complement(2931..>4852)
                     /gene="ligA"
                     /locus_tag="VGC27_13805"
     CDS             complement(2931..>4852)
                     /gene="ligA"
                     /locus_tag="VGC27_13805"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY0283683.1"
                     /translation="VHRVPMLSLDNAFAGEDVTDFAARIRRFLGLKEEDDLVFTAEPK
                     IDGLSASLRYENGVFVQGATRGDGAEGEDITANLRTLKDVPLRLAGQPPGVLEVRGEV
                     YMTHADFAALNRRHEAEGEKLFANPRNAAAGSLRQLDPSITAARPLHFFAYAWGEVST
                     LPEKTQWDMLQAFKSYGFQVNEEIRRCKKTDELLKFYRDIEAKRAKLGYDIDGVVYKV
                     DDLGLQERLGFVSRSPRWAIAHKFAAEQAETILEGIDIQVGRTGKLAPVARLKPVTVG
                     GVVVSNATLHNEDEIARKDVRLGDTVVVQRAGDVIPQIVRVILEKRPKTAKPYQFPDK
                     CPACGSHAVREVDLKTGKEDVDRRCTGGLICPAQAVERLKHFVSRDAFDIEGLGETMI
                     QLFHAKGLLKEPADIFALPRQADRVSRLLAEHRASLSAERREREGKEPVRKKAKKDEE
                     KEDKVVENLVASIDRRRDISLDRFINALGIRHVGETTAKLIARNFRDLDAFITAMESD
                     HAIVDLDSIEGVGETVAEAIKDFFDEPHNRAVLDHLTKREELKVLPVEAAKTSGSPVA
                     SKTVVFTGTLEKMTRHEAKARAEALGAKVASSVSKKTDLVVAGPNAGSKLADAQKFGV
                     EVIDEDAWLKLIGGG"
BASE COUNT          905 a         1513 c         1556 g          878 t
ORIGIN      
        1 aatacgtccc caagcgcgcc gagcggctgg cttggctgac cgccttcacc ggctcggccg
       61 gcgtggcggt ggtgctggcc gacaaggccg cgatcttcgt ggacgggcgc tacacgctcc
      121 aggtgcgcaa gcagacggac acgagcctgt tcgagccgcg cgatctggtg accgaaggac
      181 cggcggcatg gatcgaggcc aatgctaagg gcgccaagct cggatatgac ccttggctgc
      241 acacggcagc cgtggtggaa ggcttgaagg cttcggcgaa aaaggccggg gccacgctgg
      301 tcgcctgcga taccaatccc atcgacgcgg tgtggagcga ccagccggac gcgcccgccg
      361 ccaaggcggt gccgcacggc ttgccgctgg cgggcgagag cgccgaagcc aagcgcatac
      421 gcatcgccga agaggtaaag aaaaaaggcg ccgacgccac cgtgctgacc atgccggact
      481 cgatctgctg gctgctcaac atccgcgggg cggacgtgcc gcacacgccg ttcgcgctcg
      541 ccttctccat tttgaacgaa gacggttcga ccgacctgtt catggacgag cgcaaaagct
      601 cgccggaact ggcgctgcat ctgggcaatg cggtgcggct gcgcgcgccg aacgaatttg
      661 cgcccgcgct ggatgcgctg tccggcaaga cggtggcggc cgatcccggc accgccgccg
      721 cggcgatctt cgaccggctg ggcaaggcgg gagcgaaaat cctgcatctc tccgacccct
      781 gccaattggc gaaggcctgc aagaattcca tcgaggtcga aggcgcgcgc aaggcccata
      841 tccgcgacgg cgcggcgctg acgaagttcc tctgctggtt cgcacgcgag gcgcccaagg
      901 gcaagctgga cgagatcggc gcctccgcaa agctggagga gttccgcaag gcgaccggcg
      961 cgctatccga cctttccttc gattccattt cggcggcgtc ggaacacgcg gcgatccccc
     1021 attaccgcgt gacccgctcg tccaacctgc cgatccggca aggcgagatt tatctcatcg
     1081 attccggcgc gcaatatccc gacggcacca cggacgtgac ccgcaccgtg atcgcgggaa
     1141 cgccgagcgc ggaaatgaaa gaccgcttca cccgcgtatt gaagggccat atcgcgctcg
     1201 ccaccgcgaa gtttccggaa ggcaccaccg gcgcggcgct cgattccttc gcgcggcggc
     1261 cgctgtggga tgcggggctg gactacgacc acggcaccgg ccacggcgtc ggcagctttc
     1321 tctcggtgca tgaagggccg cagagcatct ccaagcggca agtgacgcag gcgttgaagc
     1381 cgggcatgat cgtttccaac gagccgggct attacaaggc gggcgaatac ggcatccgca
     1441 tcgagaatct gatcgtggtg cgcgcgcttg gcctggcgcc gggcggcgac cgccagatga
     1501 tggagttcga aaccatcacg ctcgcgccca tcgacctcaa tctggtggaa gtctcgctgc
     1561 tgacggcgga ggaacgcgcc tggctcaacg actatcacgc ccgcgtgcgc gaaaagctga
     1621 cccggctggt ggacgacgaa acgcgggcgt ggctggaaca ggcaacaagg gggatttgaa
     1681 tgatcgtcga gatgcggaca tacaaggtca aacgcggcgc gcgcgacaag ttcatcgaag
     1741 tgctgcgtac cacgggcttc gcggaactca agcgaatcgg cgtgaagtgc gccgggccgt
     1801 tcgcctcgat cgaggacgag acgacgatct tctggatgcg cggcttcccc gacgaggcct
     1861 cgcgcggccc gatgtcgaac cagttctacg gcggcgacgc ctggaagaac gaactggccg
     1921 acgccttcat gccgaacctc gaaaagtacg acgtggtggc ggtggaaatg cccgagagcg
     1981 cggtgcagtg ggtgtgaagc gacaagggcg gcttagaact gattgtggtc agacggaatc
     2041 aaatactaat ttgtcatggc ccgcgagttc gtagcagcag cgtacgaacg ggccatccag
     2101 tagaaatccg aagaaatctt tgaagaatag cgcagcaaaa cccttgggag gcgcgtagat
     2161 gtcacctggg tagcccgcat tcgcgggcca tgacaccggg gtgacatgag tcacctcgat
     2221 gccaatctgc tctaggcgcg gctttcgagc gggcggacga acttttcgaa atcagtgcct
     2281 gctgttgcct gcaaaagccg cctgtccgcg gtgaccaact cgcagccttc gcgctccgcc
     2341 aaggcgatat agaacgcgtc atacgcagga tagtggagcg ttgtcgcaac gtgccatgcc
     2401 tgagcggcaa gcaaaaccga cggaacgagc ttcgaaaaag gctccgccaa agcatcgacg
     2461 gaacgttcaa gctgatcgac cggcgccagt ttcttttgaa acgctttcca aagcgcattc
     2521 accagctcgg cgacgatgat gtccggggcg atcaactcat gtccggcctg caagcggtcg
     2581 gcttcgccgc tgccttcttc cgccagaaac catttcagtg caacactcgc atcgacgacc
     2641 agcaggctca tcgctcatcg cgtccttcac ggatcaaatc ctccgcaaaa gggggcgcgg
     2701 tgcgcagggg cgacatttcc cgaatgctgc gcagcttcgc gagcacctcc tctttcgaag
     2761 gacgcgcgat ttcggcaagt tgccggcgca gcgattcctc cagcgagagc ccctgttcgg
     2821 ccgcgcgctt cttcagttgc gccaagacgg catcgtcaag attgcggatg atgatttggc
     2881 ccacagacgc ctccatgcta tcacgatgat agcacaaatg cggcgggctg tcatccgccg
     2941 ccgatcagct tcagccacgc atcttcgtcg atcacttcga cgccgaattt ctgggcgtcg
     3001 gcgagcttgg agcccgcgtt gggcccggcg acgacgagat cggttttctt ggagaccgag
     3061 ctggcgacct tcgcgcccag cgcttcggcg cgcgccttgg cttcgtggcg ggtcatcttt
     3121 tccagcgtgc cggtgaagac tacggtcttg cttgcgaccg gcgaaccgga cgttttcgcg
     3181 gcttcgaccg gtagaacctt gagttcttcg cgcttcgtca aatgatcgag caccgcgcga
     3241 ttgtgcggct catcgaagaa atccttgatc gcttcggcga ccgtctcgcc caccccttcg
     3301 atggaatcga gatcgacaat ggcgtgatcg ctttccatcg cggtgatgaa ggcgtccagg
     3361 tcacggaaat tgcgggcgat gagcttggcc gtggtctcgc cgacatggcg gatgccgagc
     3421 gcgttgatga aacggtcgag cgaaatatcg cggcggcggt cgatggaggc gacgaggttc
     3481 tcgacaacct tgtcttcctt ctcctcgtcc ttcttcgcct ttttcctgac gggttccttg
     3541 ccttcccttt cgcggcgctc ggcactcagg ctggcgcgat gttcggcgag aagccggctc
     3601 acgcggtcag cttggcgagg cagagcgaag atgtcggcag gttccttgag cagtcctttc
     3661 gcatggaata gctggatcat ggtttcaccc agcccttcga tgtcgaaagc gtcgcgcgag
     3721 acgaaatgtt tgagccgctc caccgcctgg gccgggcaga tcaacccgcc ggtgcagcgc
     3781 cggtccacgt cttccttgcc ggtctttaag tccacctcgc gcaccgcatg gctgccgcag
     3841 gccgggcatt tgtcgggaaa ttggtagggc ttggcggttt tcggacgctt ctccaggatc
     3901 acgcgcacga tctggggaat gacgtcgccg gcgcgctgga ccacgacggt gtcgccgagg
     3961 cgcacatcct tgcgggcgat ctcgtcctca ttgtgcaagg tggcgttcga caccaccacg
     4021 ccgcccaccg tgacgggctt gaggcgggcg accggggcga gcttgccggt gcggccgacc
     4081 tggatgtcga tgccttcgag gatcgtttcg gcctgttcgg cggcgaattt atgggcgatg
     4141 gcccagcgcg ggctgcgcga aacgaagccc agccgttcct gaaggccgag atcgtccacc
     4201 ttatagacca cgccgtcgat gtcgtagccg agcttggcgc gtttggcttc gatgtcgcga
     4261 tagaatttca acagctcgtc cgtcttcttg cagcggcgaa tttcttcgtt gacctgaaag
     4321 ccgtaggatt tgaacgcttg cagcatgtcc cattgggttt tctcgggaag cgtgctcacc
     4381 tctccccagg cataggcgaa gaaatgcagt ggccgcgcgg cggtgatcga cggatcgagc
     4441 tggcgcagcg agcctgcggc cgcattgcgc ggattggcga agagcttctc gccttccgcc
     4501 tcatggcgcc ggttcagcgc cgcgaaatcg gcatgggtca tatagacctc gccgcgcact
     4561 tccagcacgc cgggcggctg gccggcgagg cgcaacggaa cgtccttaag ggtgcgcaga
     4621 ttggcggtga tgtcttcgcc ctcggcgccg tcgccgcgcg tggcgccctg cacgaagaca
     4681 ccgttctcat aacgcagcga ggcggacagc ccgtcgatct tcggttcggc tgtgaaaacc
     4741 agatcgtcct cctccttcag ccccaggaag cggcgtatgc gggcggcgaa atcggtcacg
     4801 tcctcgccgg caaaggcatt gtcgagcgac agcatcggca cgcgatggac ga
//
LOCUS       DASWPU010000647         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_48729,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000647 DASWPU010000000
VERSION     DASWPU010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48729"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VGC27_13810"
     CDS             <1..404
                     /locus_tag="VGC27_13810"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY0283684.1"
                     /translation="DLMYAVVAEVEKYPEFLPWCVALRVLSREHVKDRDVLRAEMLVG
                     FGALRERYTSRVVLDKAAHSIDVVQTAGPFRQLENHWRFTPEGEGCSVDFSITFEFKN
                     RLLDTVAGKAFELVLLKMTAAFETRAKALSH"
     gene            complement(396..884)
                     /locus_tag="VGC27_13815"
     CDS             complement(396..884)
                     /locus_tag="VGC27_13815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY0283685.1"
                     /translation="MFSPALLSLAKTVLDEARANKLRLATAESCTGGLIAGLLTEIPG
                     SSDVLERGFVVYSNKAKRELLGVPGALITHDGAVSKSVVCAMAEGALARADAELAVAV
                     TGVAGPGGGTAEKPVGLVHFAFCRKGGATLHEEHRFGDIGRTQVRLETVKTALELVRR
                     LM"
     gene            complement(884..1336)
                     /locus_tag="VGC27_13820"
     CDS             complement(884..1336)
                     /locus_tag="VGC27_13820"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY0283686.1"
                     /translation="MSLAGNCAILFGVGRVPLMPGTAASAVALPFAWLIATAFGPVAL
                     LLAAVAVGLLAVPISGAYARETGREDPSDCVIDELAGQWLACACAPLTLWGFALAFLL
                     FRLFDMTKLWPVSAAEELKGGWGIVADDMVAGGLAGVIVLAAVHFGLL"
     gene            complement(1333..2484)
                     /locus_tag="VGC27_13825"
     CDS             complement(1333..2484)
                     /locus_tag="VGC27_13825"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HEY0283687.1"
                     /translation="MPRTAVLIVAAGKGERAGTSTPKQYVPLLGQSILRRSIEAFGGL
                     GADCVIKVAIGPGQEALYAEAIAGLDLLPPIVGGATRQDSVRRGLEALAGDAPEFVLI
                     HDAARPLVSPRLIGGVIGALQAGAEAAIPLLPVADTLRKQENGKWITVPREGLLRAQT
                     PQGFRFAAILKAHRDHAAHEVTDDMALAELAGLNIAAVPGEETNMKVTNAEDFTVAEG
                     LLSARLGETRTGSGYDVHRFVPGDHVWLCGVKVPHDRALEGHSDADAGLHALTDALLG
                     AIGEGDIGLHFPPTDERWRGAPSWTFLAHAADLVRGKGGAIVHCDVTIVCERPKLAPH
                     REAMRAKIAEILHLDLSRVSVKATTTEGLGFTGRREGLAAQAVATVRLP"
BASE COUNT          481 a          890 c          841 g          445 t
ORIGIN      
        1 ccgacctgat gtacgccgtg gtggccgagg tggaaaaata tcccgaattc ctgccttggt
       61 gcgtcgccct gcgggttctg tcgagagagc atgtgaagga tcgcgacgtg ctgcgcgccg
      121 agatgctggt cggcttcggc gcgctgcgcg agcgctatac cagccgcgtc gtgctcgata
      181 aagcggcgca cagcatcgat gtggtgcaga ccgccgggcc gttccggcaa ttggaaaacc
      241 attggcgctt tacgccggaa ggcgagggtt gcagcgtgga tttctccatc accttcgagt
      301 tcaagaaccg cctgctcgat accgtcgccg gcaaggcttt cgaactcgtg ctgctgaaaa
      361 tgaccgctgc tttcgagacg cgggcgaagg cgctgtcaca ttagccggcg caccagttcc
      421 agcgctgttt ttaccgtttc caggcgaacc tgcgtcctgc cgatgtcgcc gaagcgatgt
      481 tcttcgtgca gcgttgcgcc gcctttgcga cagaatgcga aatgcaccag ccccacgggt
      541 ttttcggccg tgccgccgcc cggcccggca acgccggtaa cggccacggc caattcggca
      601 tcggcgcggg ccaaagcgcc ttccgccatc gcacagacca cggatttgga cacggcgccg
      661 tcatgggtga taagtgcgcc tggtacgccg agcaactccc tcttcgcctt gttcgaataa
      721 accacgaagc cgcgctccag cacatccgac gaaccgggaa tctcggtgag caggcccgcg
      781 atcaagccgc cggtgcagct ttccgccgtg gcgaggcgca gcttgttcgc gcgcgcctcg
      841 tccagcaccg ttttcgccag cgaaagaaga gcaggggaga acatcacagc agtccgaaat
      901 gcacggccgc aagcacgata acacccgcca gcccgcctgc caccatgtcg tcggccacga
      961 tgccccaacc gcctttcagt tcttccgccg ccgacaccgg ccacagcttc gtcatgtcga
     1021 acaggcggaa caacaagaac gccagcgcga agccccacaa ggtcaagggc gcgcaggcgc
     1081 aggccagcca ttgcccggcc aactcatcaa tcacgcagtc ggaagggtcc tcgcgtccgg
     1141 tttctctcgc ataggccccg gaaatgggaa cggccaacag cccaaccgcg accgcggcaa
     1201 gcagcaatgc tacaggtccg aacgccgtcg cgatcagcca agcgaagggc agggcaaccg
     1261 cgctcgccgc cgttcccggc atgagcggca cgcggccgac gccgaacagg atggcgcaat
     1321 ttcccgccag gctcacggca gccgcaccgt cgccacggcc tgcgccgcca gtccttcgcg
     1381 ccgcccggtg aagcccaggc cttccgtcgt cgtcgccttg acgctgacgc gcgagaggtc
     1441 gagatgcaga atctcggcga tcttcgcgcg catcgcttcg cgatgaggtg cgagtttcgg
     1501 gcgctcgcag acgatcgtga cgtcgcaatg gacgatggcg ccgcccttgc cgcgcaccaa
     1561 gtccgctgca tgcgcgagga acgtccacga cggcgcgccg cgccagcgtt catccgtagg
     1621 cggaaaatgc aggccgatgt cgccttcgcc gatggcgccc aggagcgcgt cggtcagggc
     1681 gtgcaggccg gcgtcggcgt cggaatggcc ttccagcgcg cgatcgtgcg ggactttcac
     1741 gccgcacagc cagacgtgat cgcccggcac gaagcgatga acgtcgtagc ccgaccccgt
     1801 gcgcgtttcg cccagccgcg cggacagcag accttcggcc accgtgaaat cctccgcgtt
     1861 cgtcactttc atgttggttt cctcgcccgg cacggcggcg atgttcaagc ccgccagttc
     1921 ggccagcgcc atgtcgtcgg tcacttcgtg cgcggcgtga tcgcgatgcg ccttcaggat
     1981 cgccgcgaag cggaagcctt gcggcgtctg cgccctgagc agcccctcgc gcggcaccgt
     2041 gatccacttg ccgttctctt gtttgcgcag cgtatcggcg acgggaagca aagggatcgc
     2101 cgcctccgcg ccggcctgga gagcgccgat cacgccgccg atcaagcgcg gcgaaacaag
     2161 ggggcgtgcc gcatcgtgga tcagcacgaa ctccggtgcg tcgccggcca aagcttcgag
     2221 cccgcggcga acggaatcct gccgcgtcgc gccgccaacg atcggcggca gcaaatcgag
     2281 accggctatt gcctcggcgt agagtgcttc ctggcccgga ccgatcgcga ctttgataac
     2341 gcagtccgca ccaagcccgc cgaaggcctc gatggagcgg cgcagaatcg attgccccag
     2401 cagtgggaca tattgtttgg gggtgcttgt ccccgcgcgt tcgcccttgc cggcggcgac
     2461 gatcagaaca gcagttcgcg gcatgaaggc tccggattcg caggctttct tggtagcacg
     2521 gggacgcagg tctgaggggg cctgggtgtg ttttaccaat cattttccca agatggcgac
     2581 ttggggacgc agagggtata aagaggggcg atacggcgga aatggcactt ttttttcgcc
     2641 atagacagta gttgcac
//
LOCUS       DASWPU010000648         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_79120,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000648 DASWPU010000000
VERSION     DASWPU010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79120"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /gene="nuoN"
                     /locus_tag="VGC27_13830"
     CDS             <1..1303
                     /gene="nuoN"
                     /locus_tag="VGC27_13830"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004440.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY0283688.1"
                     /translation="LMAVAAYLVLTSPDAGAFHGAFVTDGFARFAKALILLSAALCTM
                     MAQSFFEDEGRVRFELPVLIALSTLGMMLMVSASNFISLYLGLELQSLALYVLAAFNR
                     DSARSTEAGLKYFVLGALSSGMLLYGISLIYGFTGTVDFAGVAFAVKLSGVGIGLIFG
                     LVFLIAGLAFKLSAVPFHMWAPDVYEGAPTPVVAFFSSAAKVAAFALFIRAILVPFPD
                     ALHQWQQVIVVVSVLSMALGAVAAIGQENIKRLMAYSSIGHMGYGLLGLAAGTELGVQ
                     GMLFYLAFYAVTNIGVFVCIQAMRRDGEAVETIADLAGLARTRPRMAFALAALMLSLA
                     GLPPLAGIFAKIYVFLAAVEAHLYIPAVLGFLASAVGAYYYLRIVKVMYFDEPARPFD
                     TVMGRGMGAILALSSLFTILFVFLPGPLVDAAGIAAQALFR"
     gene            1300..2043
                     /locus_tag="VGC27_13835"
     CDS             1300..2043
                     /locus_tag="VGC27_13835"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY0283689.1"
                     /translation="MSTAWPPGYALLELDEIDSTNEEARRRAAAGEERPLWIAAARQS
                     AGRGRRGREWRSAPGNLFATLLLRPEKPAGECAQLSFVAALAVSDMLARFAPHTEFFL
                     KWPNDVLAGGRKIAGILLESENGADGRTAWLAAGIGVNLTVYPEDTEFPAVSLAALGA
                     VPPAPKDALLHLAARFAKWYEVWRSAGFAPIREAWLARASGLGARIRVRLTNEEIMGV
                     FQGIDGSGALLLGLPGGTTREIAAGEVFL"
BASE COUNT          275 a          665 c          715 g          424 t
ORIGIN      
        1 gctgatggcg gtggcggcct atttggtgct gacgtccccg gatgcgggcg cattccacgg
       61 cgccttcgtc accgacggct tcgcgcgctt tgccaaagcg ctgatcctgc tctcggcggc
      121 gctgtgcacc atgatggcgc agagcttctt cgaggacgaa ggccgggtcc gcttcgaact
      181 gccggtgctg attgcgcttt cgaccctcgg catgatgctg atggtgtcgg cctcgaactt
      241 catttcgctg tatctcggcc tggaactgca aagcttggcg ctttacgtgc tggcggcttt
      301 caaccgcgac agcgcgcgct cgaccgaagc gggcctgaaa tatttcgtcc tgggggcgct
      361 gtcctccggc atgctgctct acggcatttc gctgatctac ggcttcaccg gcacggtgga
      421 tttcgccggc gtggcgttcg cggtgaaact gtcgggcgtc ggcatcggcc tgatcttcgg
      481 gctggtcttc ctgatcgccg ggctggcgtt caagctctcc gccgtgccgt tccatatgtg
      541 ggcgcccgat gtgtatgaag gcgcaccgac gcccgtcgtg gccttcttca gttcggccgc
      601 gaaagtggcc gccttcgccc tcttcatccg cgcgatcctg gtgccgttcc cggatgcgct
      661 gcaccagtgg cagcaggtga tcgtcgtggt gtcggtgctg tcgatggcgc tgggggcggt
      721 ggcggcgatc ggccaggaga acatcaagcg gttgatggcc tacagttcca tcggccatat
      781 gggctacggg ctgctcggtc tggcggcggg aaccgaactg ggcgtgcagg gcatgctgtt
      841 ctatctcgcc ttctacgcgg tcacgaatat cggcgtgttc gtctgcatcc aggcgatgcg
      901 gcgcgacggc gaggccgtgg aaaccatcgc cgatctcgcc gggctggcgc ggacgcgccc
      961 gcgcatggcc ttcgccctgg cggcgctgat gttgagcctg gcgggcctgc cgccgctggc
     1021 cggcatcttc gccaagatct acgtcttcct ggcggccgtg gaagcgcatc tttacatccc
     1081 cgccgtgctg ggcttcctcg ccagcgccgt gggcgcctat tactatctgc gcatcgtcaa
     1141 ggtgatgtat ttcgacgaac cggcgcggcc cttcgacacc gtcatgggcc gcggcatggg
     1201 agccattctg gcgctttcca gcctgttcac gatcctgttc gtgttcctgc cggggccgct
     1261 ggtcgatgcg gcggggatcg ccgcgcaggc cctgttccgg tgagtacggc atggccgccc
     1321 ggttacgcgc tgctggaact cgacgagatc gactccacca acgaggaagc gcgccgccgg
     1381 gcggcggcgg gcgaggagcg tccgctgtgg atcgccgccg cgcgccagag cgcggggcgt
     1441 ggaaggcggg gcagggagtg gcgatcggcg cccggcaatc tcttcgccac acttctgctg
     1501 cgtcccgaaa aaccggcggg cgaatgcgcc cagctttcct tcgttgccgc tttggccgtc
     1561 tcggacatgt tggcgcgctt tgccccgcat accgaattct tcctgaaatg gcccaacgac
     1621 gtgctcgccg gcgggcgcaa gatcgcgggc atcctgttgg aatccgaaaa cggcgctgac
     1681 ggaagaacgg cctggctcgc cgccggcatc ggcgtcaacc ttaccgttta ccccgaagac
     1741 acggagtttc cggcggtttc gctggccgcg ctcggcgccg tgccgcccgc gccaaaggac
     1801 gcattgctgc atctcgctgc ccggtttgcg aaatggtatg aggtctggcg gtcggccggc
     1861 ttcgcgccca tacgcgaggc gtggctggcg cgcgcgtccg ggctgggagc gcgcatccgc
     1921 gtccggctga ccaacgagga gatcatgggc gtgtttcagg gaatcgacgg cagcggcgct
     1981 ctgcttctgg gccttccggg cgggacgacg cgagagatcg ccgcgggaga ggtgttcttg
     2041 tgatggcgcg cattagtttt tctccccccg tttacgggg
//
LOCUS       DASWPU010000649         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_48803,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000649 DASWPU010000000
VERSION     DASWPU010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48803"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VGC27_13840"
     CDS             complement(<1..587)
                     /locus_tag="VGC27_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HEY0283690.1"
                     /translation="MDRVAAAVADRDVRYLAASSETASELNFSGTDWKREINVRDFIQ
                     HNVTPYLGDESFLAPPTERTKALWAVARKLMDEEHAKGGTLDMDTEIVSTIVSHQPGY
                     ISKELEQVVGLQTDKPLKRAFMPFGGIRVAESALEAYGYKAAPQLHDFFTHYRKTHND
                     GVFDVYTDPVRKARKAGIITGLPDGYGRGRIIGDYR"
     gene            816..1079
                     /locus_tag="VGC27_13845"
     CDS             816..1079
                     /locus_tag="VGC27_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283691.1"
                     /translation="MENTGRPVVERVQTGIRIEKRILKVLKGLAEAFDITLGDLLEGM
                     VLHNFEGKCPFRPETIQLIEKLKVVYGLDLDASASHHMVERKP"
     gene            1398..>2744
                     /gene="ahcY"
                     /locus_tag="VGC27_13850"
     CDS             1398..>2744
                     /gene="ahcY"
                     /locus_tag="VGC27_13850"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY0283692.1"
                     /translation="MAAQYVVKDIALADWGRKELAIAETEMPGLMATRAEFGASKPLK
                     GARIAGSLHMTIQTAALIETLKVLGADVRWASCNIYSTQDHAAAAIAAGGTPVFAVKG
                     ESLPDYWDYTHRIFEWADGGTPNMILDDGGDATLLVHLGVRAESGDTAFLDKASNEEE
                     EALFASIKQRLQTKPGWYGAIAKNIRGVTEETTTGVHRLYQMEKDGKLLFPAINVNDS
                     VTKSKFDNLYGCRESLVDGIRRGTDVMMAGKVAMVAGFGDVGKGSAASLRQAGCRVLV
                     SEIDPICALQAAMEGYEVVTLEDAAPRADIFVTCTGNVDVITVDHMRAMKDRAIVANI
                     GHFDSEIQVGGLRNFKWHNVKPQVDEVEFPGGKRIILLSEGRLVNLGNAMGHPSFVMS
                     ASFTNQTLAQMELFGNPGKYEKKVYVLPKHLDEKVARLHLEKIGVKLTTLSDRQSAY"
BASE COUNT          560 a          830 c          832 g          522 t
ORIGIN      
        1 cggtagtcgc cgatgatccg cccgcggccg taaccatcgg gcagtccggt gatgatgccc
       61 gccttgcgcg ccttgcgcac cggatcggtg taaacgtcga acacgccgtc attgtgggtc
      121 ttgcggtagt gggtgaagaa atcgtgcagt tgcggggcgg ccttgtagcc gtaagcctcg
      181 agcgcgcttt ccgccacgcg gatgccgccg aaaggcatga aggcgcgctt gagcggcttg
      241 tcggtctgca ggccgacgac ctgctccagt tccttgctga tgtaaccggg ctggtgcgaa
      301 acgatggtgg aaacaatctc ggtatccatg tcgagggtac cgcccttggc gtgctcctcg
      361 tccatcagct tgcgagcaac ggcccacagc gccttggtgc gttcggtcgg aggcgccaga
      421 aacgattcgt cgccgagata gggcgtcaca ttgtgctgga tgaaatcgcg gacgttgatc
      481 tcgcgcttcc agtccgtgcc ggaaaaattc agctccgaag cggtttcgga gctggcggcg
      541 aggtaacgga catcccgatc tgcgacagct gctgcaactc tatccacggt ttcaactcct
      601 cggtaacggt cgccgctcac gttcgggcgg cacggcgttc tctatctcgg tctacgagcc
      661 tgtttagcat ataggtgcaa aatggaagca ttataagagc gtcaatttgc catgcgtgcc
      721 ggccctagga agcgcggcga aatcgagcgc cgcacttacg ggaagaggcc ggaaaaccac
      781 ttgacaatac atatatacat tattatatag ataccatgga aaacacgggg cggcctgtcg
      841 tcgagcgcgt gcagacaggc atacgcatcg aaaagcgcat cctgaaggtc ctgaaggggc
      901 tcgccgaagc tttcgacatc acgcttggcg atcttctgga gggcatggtc ctgcacaatt
      961 tcgaaggcaa atgtccgttc cggcctgaga ccattcagct catcgaaaag ctgaaagtgg
     1021 tttacggact agatctcgat gccagcgcca gccaccacat ggttgaacgc aagccatagg
     1081 cccaacgata taattcttcc gttgcgcgtc gcatgccggt gcgcgctgtc cgcgatcagc
     1141 gaattgatcg ggtctctgag atgaccgtac tccggccgtt tcagcgccgg ccgttttgcc
     1201 gcatctgtgg aattcccttt ttcccgtggc tttttgaggc ccggttaggc accggataag
     1261 catatgcata tttctttatg tgtttctaga cagacccgga accggctgct atatcccgcc
     1321 cgctatcaac gccctctgag cctgccgaag ggtcatgctt cgacaagctc tgagggtttt
     1381 tcaaaggatc gcccgccatg gccgctcaat acgtcgtcaa ggacatcgcg ctggccgatt
     1441 ggggacgcaa ggaactcgca atcgccgaga ccgagatgcc cggcctgatg gccacgcgcg
     1501 ccgaattcgg cgcctccaag ccgctgaaag gcgcgcgcat cgccggctcg ctgcatatga
     1561 cgatccagac cgccgcgctg atcgagacgc tgaaagtgct gggtgcggac gtgcgctggg
     1621 cctcgtgcaa catctattcg acgcaggatc atgccgcggc ggcgatcgcc gcgggcggca
     1681 cgccggtctt cgcggtcaag ggcgagagcc tgccggacta ttgggactac acccaccgca
     1741 tcttcgaatg ggccgacggc ggcacgccca acatgatcct cgacgatggc ggcgacgcca
     1801 ccctgctcgt ccatctcggc gtgcgcgcgg aaagcggcga caccgccttc ctcgacaagg
     1861 cgtcgaacga agaagaggaa gcgctgttcg cttcgatcaa gcagcggctt caaaccaagc
     1921 cgggctggta tggcgccatt gccaagaaca tccgcggcgt gaccgaggaa accaccaccg
     1981 gtgtgcatcg cctctatcag atggagaagg acggcaagct gctgttcccc gccatcaacg
     2041 tcaacgacag cgtcaccaag tcgaaattcg acaacctcta cggctgccgc gagtcgctgg
     2101 tggacggcat ccgccgcggc accgacgtga tgatggccgg caaggtggcg atggtcgccg
     2161 gcttcggcga cgtgggcaaa ggctccgccg cttcgctgcg ccaagccggc tgccgggtat
     2221 tggtcagcga gatcgatccg atctgcgcgc tccaggccgc catggaaggc tatgaagtcg
     2281 tcaccttgga agacgccgcg ccgcgcgccg atatcttcgt cacctgcacc ggcaatgtgg
     2341 atgtcatcac cgtggaccat atgcgggcca tgaaggaccg cgccatcgtc gccaacatcg
     2401 gccatttcga ttccgaaatt caggtcggcg gcctgcgcaa tttcaaatgg cacaacgtca
     2461 agccgcaggt ggacgaagtg gaatttcccg gcggcaagcg catcatcctg ttgtcggaag
     2521 gccggctggt gaacttaggc aacgccatgg gccatccgag cttcgtgatg agcgcgtcgt
     2581 tcaccaacca gacgctggcg cagatggagc tgttcggtaa tcccggcaaa tacgagaaga
     2641 aggtctacgt cctgcccaaa cacctcgacg agaaggtcgc gcgcctgcat ctggagaaga
     2701 tcggcgtcaa gctgaccacg ctgagcgaca ggcagtccgc ctac
//
LOCUS       DASWPU010000650         6992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_11300,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000650 DASWPU010000000
VERSION     DASWPU010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11300"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VGC27_13855"
     CDS             <1..784
                     /locus_tag="VGC27_13855"
                     /inference="COORDINATES: protein motif:HMM:NF039288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY0283693.1"
                     /translation="ILFGNEKVVATPHLGASTEEAQENVALQVAEQIADYLLTGAIIN
                     ALNMPSIGAKEAALVRPWITLAERLGAFAGQLTDTSIEAVEILYEGAAANLNQRALTQ
                     AALAGLLKPHLPEVNMVNAPVVAKERGIKVSETRRDRQGVYEGYIKISVTTPEMTRGV
                     AGTVFSDGRPRLIQVKDINLDAEFAPHMLYVTNEDKPGFIGRLGTALGEAKVNIANFT
                     LGRTAPGADAIALVEVDGGVSEKVLADIRKLPLVRQAKALAF"
     gene            921..1529
                     /locus_tag="VGC27_13860"
     CDS             921..1529
                     /locus_tag="VGC27_13860"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY0283694.1"
                     /translation="MAPLVAIYLLWGMWFLSWVCSRIWVSPTIKRAGTGREILYRVMM
                     LIGIVLLFGLYSNGYEIQHRFWDPLRGALGWAMLVPVVAGLSFCWWARIHLGPLWSGA
                     ITRKNNHHIVDTGPYALVRHPIYAGTMLAVLATAFLNGTPSSFLGAALMALGFYTKAR
                     REERFLREELGPDAYDAYAKRVPMLVPFLKRAVAAVHMAKRR"
     gene            1599..3014
                     /locus_tag="VGC27_13865"
     CDS             1599..3014
                     /locus_tag="VGC27_13865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0283695.1"
                     /translation="MAAEDSVLRNEERARERNAKLALDQTALSLRSLALNHWIMPVFT
                     ATVCVMFARWIEPLRLAVWFAVVTVSTVPLAMVCRMFRRHKPDASRRQYWVAAAAGSY
                     FVFTAAWASLGFVLWAPNDSLNHMVVLLILASTIAGSSALTSASKPLALVAFATYGTV
                     LVLTPLQQGGAVYKGLSLLAVFFTLYLVYMWRHMHETARDMLLLRNDKDDLIVALARA
                     KTESDEACARAEAANKAKSQFLANMSHELRTPLNAILGFSEIIFTNAVHGDTAKDHDY
                     AKLINKSGHHLLTLINDVLDLAKIEAGGLTLRDAELDLDRIIDEAHALMTPKAEAGGC
                     LLAKEIGASLPYLFADERAVKQVLLNMLSNAVKFTRAGGTVTSFVRVEDDGRIALGVS
                     DTGVGIAKEDQALVFQKFGQGRHDIVTEQGTGLGLPIVKGLVEAHGGSIVLESETGVG
                     TAITARFPASRARPARLRAAS"
     gene            complement(3181..4233)
                     /locus_tag="VGC27_13870"
     CDS             complement(3181..4233)
                     /locus_tag="VGC27_13870"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0283696.1"
                     /translation="MLKTLAYAAPAAKSQLAPYSFERREPKEHDVVIEIKYCGICHTD
                     VHQVRDEWGGGIFPMVPGHEIAGIVTRVGSHVTKFKPGDKVGIGCLVDSCRVCEHCRD
                     HLEQYCLNGVSLTYSGYEQDKKTITQGGYSDKIVTDENFVLKIPDNLALDAAAPLLCA
                     GITLYSPLVHWKAGPGKKVAILGLGGLGHMGVKIAHALGAEVTVLSHSLKKQADAKRL
                     GADHFYATSDPETFAKLKGSFDLIVCTVGAGIDWNQYLELLKLDATMVIVGVPAEQVP
                     LGAFPLLSLRRSLAGSMIGGIKETQEMLDFCGKHDIVSDIELIPIQKVNEAYDRIVKS
                     DVRYRFVIDLASLKAA"
     gene            4332..4706
                     /locus_tag="VGC27_13875"
     CDS             4332..4706
                     /locus_tag="VGC27_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0283697.1"
                     /translation="MKPLSHPHIDDVTVEGILHAFSSPIRIQIYAQLVAADCPQCCSA
                     YLRIKGGKLPKSTLSQHFAVLREAGLIRSERKGNELQNTTRCDELEKRFGAMIDAVLD
                     AYLAQRTARKKKRRRRSSLTPP"
     gene            complement(4693..5490)
                     /locus_tag="VGC27_13880"
     CDS             complement(4693..5490)
                     /locus_tag="VGC27_13880"
                     /inference="COORDINATES: protein motif:HMM:NF006699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY0283698.1"
                     /translation="MSVLLYEKHGFVATLTINRPESRNALGEEGDGELFAAAAAKINA
                     DRELRCAILTGAGTAFSAGGNVKAMREKSGIFAGTPDQVRESYKREIHQIVRSLWGLE
                     VPLIAAVNGPAIGLGNDIACLADIRIASRFAVFGTPFLKIGLVPGDGGAWILPRVIGQ
                     AHAAELFFIADTIDAETALKWGLVSRTVPPEMLMNEARGLAAEICRQPPGVLRMTKKL
                     MREALGADLETILERSAAFQAQAHASEDHAEALAAFFEKRDPVFKGG"
     gene            5705..6013
                     /locus_tag="VGC27_13885"
     CDS             5705..6013
                     /locus_tag="VGC27_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283699.1"
                     /translation="MFLKGAAIALALAGATFATAGTSNAAGVSFGITDNGRHNSSTAM
                     SFNFGEVAFAYQDGYWDNNRRWHKWRNRRDHDNYRKQYSNKYRNGNHTRYSGQGWQRR
                     "
     gene            6181..6534
                     /locus_tag="VGC27_13890"
     CDS             6181..6534
                     /locus_tag="VGC27_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283700.1"
                     /translation="MLIQVNTDDNVEGREKFVLYVEAEAARILSRFSDQITRLEIHLS
                     DENGDKSGSTDKRCLMEARPAGRPPVAVSHDGASLEDAWSGAAKKMLRLLESTLRRVS
                     DRKGGASIRTDGIAE"
     gene            6670..>6992
                     /locus_tag="VGC27_13895"
     CDS             6670..>6992
                     /locus_tag="VGC27_13895"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0283701.1"
                     /translation="MNSDSMMTPPFPSALEEQDAYISPEEVDSPILKHGFAYWKDLRA
                     GREFPARSDITPRGLGSLLRNALLLRVIDRGEDYEYRIVGDAHVLAHGVSVQGKLWSQ
                     VNNSK"
BASE COUNT         1330 a         2221 c         2172 g         1269 t
ORIGIN      
        1 catcctgttc ggcaacgaga aggtcgtggc gacgccgcat ctgggcgctt ccaccgaaga
       61 ggcgcaggag aacgtcgcct tgcaggtggc cgagcagatc gcggattacc ttcttaccgg
      121 cgccatcatc aacgccctca acatgccctc gatcggcgcc aaggaagcgg cgctggtgcg
      181 gccttggatc acgctggcgg aaaggctcgg cgcttttgcc ggccaactga cggacaccag
      241 catcgaagcg gtggaaatcc tctacgaagg cgccgccgcg aacttgaacc agcgcgcgct
      301 gacgcaggcc gcgctcgccg gactgctcaa gccgcacctg cccgaggtga acatggtcaa
      361 cgctcccgtc gtggccaagg agcgcggcat caaggtgagc gaaacccgcc gcgaccggca
      421 aggcgtctat gagggctaca tcaagatctc ggtcacgacg ccggaaatga cccgcggcgt
      481 cgccggaacc gtgttctccg acggcaggcc gcggctcatc caggtcaagg acatcaacct
      541 cgacgccgag ttcgccccgc acatgctcta tgtcaccaac gaggacaagc cgggcttcat
      601 cggccggctg ggcaccgccc tgggcgaagc caaggtcaac atcgccaact tcaccctggg
      661 ccgcaccgcc cccggcgccg acgccatcgc cctggtcgaa gtcgatggcg gcgtcagcga
      721 aaaggtcctg gccgatatcc gcaagctgcc gctggtccgg caggcaaaag ctcttgcatt
      781 ctgatcacac tcttgccgct ttatcgatcg tttccggccg aaacacggac gtttcggcct
      841 gaaatgcgct aataagaggg taagagcggc ttgccgattc ggcgtggccg ccggccgtcc
      901 tagtgggcgg aggggcgaaa atggcgccat tggtcgcgat ctatctgtta tggggcatgt
      961 ggttcctgtc gtgggtttgc tcgaggatat gggtcagtcc caccatcaag cgggccggca
     1021 cggggcggga aatcctctac cgggtcatga tgctgatcgg tatcgtgctg ctgttcggcc
     1081 tctactccaa cggctatgag attcagcatc gcttttggga tccgctgaga ggcgcgctgg
     1141 gctgggcgat gctggtgccg gtcgtagcgg gattgagctt ctgctggtgg gcgcggattc
     1201 atctcggccc actgtggtcg ggagcgataa cccgcaagaa caatcaccac atcgtcgata
     1261 cgggccccta tgcgctggtg cgtcatccga tctacgccgg caccatgctc gccgtgctcg
     1321 cgacggcctt tctcaacgga accccgtcgt catttcttgg cgccgcactg atggcgctgg
     1381 gattctatac caaggcgcgg cgggaagagc gtttcctgcg cgaagaactc ggaccggacg
     1441 cttatgacgc ttacgcgaag cgcgttccca tgctggtgcc gttcctgaaa cgcgccgttg
     1501 cggctgtgca catggctaag aggcgttaag caagtcccgc aagcttcttt taaccccggc
     1561 gcgacacact ggcagctcga tccggggatt agtcgccgat ggccgccgaa gactccgtct
     1621 tgcggaacga agagcgcgcg cgcgaacgca acgccaagct ggcgctcgac cagacggcgc
     1681 tatcgctgcg cagcctcgcg ctcaaccact ggatcatgcc cgtcttcacc gccaccgtct
     1741 gcgtgatgtt cgcgcgctgg atagagccgc tgcggctggc cgtctggttc gccgtcgtga
     1801 ccgtcagcac ggtgccgctt gctatggtct gccggatgtt ccggcggcac aagccggatg
     1861 caagccgccg gcaatactgg gtggccgcgg ccgccggcag ctacttcgtc tttaccgccg
     1921 cctgggcctc gctcggcttc gtgctgtggg cgcccaacga cagtctcaac cacatggtgg
     1981 tgctgctgat actggcctct accatcgccg gcagcagcgc gctgaccagc gcgtccaagc
     2041 cgctggccct ggtggcgttc gccacctacg gcacggtgct ggttctgacg ccgctgcaac
     2101 agggaggcgc cgtttataaa ggcctgtccc tgctggcggt cttctttacg ctctatctcg
     2161 tttatatgtg gcggcatatg cacgaaacgg cgcgcgacat gctgctgctg cgcaacgaca
     2221 aggacgacct catcgtcgcg ctcgcccgcg ccaagaccga atccgacgaa gcgtgcgccc
     2281 gcgccgaagc cgcgaacaaa gccaaatcgc agtttctcgc caatatgagc cacgagctgc
     2341 gcacgccgct caacgcgatt ctcggctttt cggagatcat cttcaccaac gcggtgcacg
     2401 gcgacacggc gaaggatcac gactacgcca aactcatcaa caagtccggc catcatctgc
     2461 tgacgctgat caacgacgtc ctcgatctgg ccaagatcga agccggcggg cttaccctgc
     2521 gcgacgcgga gctggacctg gaccgcatca tcgacgaagc ccacgcgctg atgacgccca
     2581 aggcggaggc gggcggctgc cttcttgcca aggagatcgg cgcctccctg ccctatctct
     2641 tcgccgacga gcgcgccgtc aagcaggtgc tgctcaatat gctgtcgaat gccgtcaagt
     2701 tcacacgggc gggcggcacg gtgacctcct tcgtccgcgt cgaggacgac ggacgcatcg
     2761 cgctcggcgt cagcgacacc ggcgtcggca tcgccaagga ggatcaggcg ctggtcttcc
     2821 agaaattcgg acaaggccgc cacgacatcg tgacggaaca aggcaccgga ctgggactgc
     2881 ccatcgtcaa gggcctggtg gaagcccatg gcggcagcat cgtgctcgaa agcgaaaccg
     2941 gcgtgggcac ggcgatcacg gcgagattcc cggcgagccg ggcgcggccg gcaaggcttc
     3001 gcgccgcgtc gtgagggttt ccgccggagc gtgatcccat ccgattgagt tgccgtccct
     3061 cacccttcga caagctcagg gtgaggggag tgcatggtcc ttcgtgccga gcccctcatc
     3121 ccgagcttgt cgagggacga agcatgtgcg cggcgcgatc ccatccggac ggatcatgcg
     3181 ctacgcggct ttcagcgacg ccaggtcgat cacgaaacgg tagcgcacgt cgctcttgac
     3241 gatgcggtcg taagcctcgt tcaccttctg gatcgggatc agctcgatat cgctgacgat
     3301 atcgtgcttg ccgcagaaat ccagcatctc ctgcgtttcc ttgatgccgc cgatcatcga
     3361 tccggcgaga ctgcggcgca agctgagcaa agggaaggcc ccgagcggaa cctgctccgc
     3421 cggcaccccg acgatgacca tcgtggcgtc cagcttgagc agttcgagat actggttcca
     3481 gtcgatcccg gcccccacgg tacagacgat gagatcgaag gagcctttca gcttcgcgaa
     3541 ggtttccgga tcggacgtcg cgtagaaatg gtcggcgccc aaacgcttgg cgtcggcctg
     3601 cttcttcagc gagtggctga ggaccgtgac ctccgcgccc agcgcgtgcg cgatcttgac
     3661 gcccatatga cccagtccgc cgaggccgag gatcgccacc ttcttgcccg gccccgcctt
     3721 ccagtgcacc agcggcgaat agagcgtgat gcccgcgcac agaagcggcg ccgccgcgtc
     3781 gagcgccaga ttgtcgggaa tcttgagaac gaagttctcg tcggtcacga tcttgtcgga
     3841 ataaccgcct tgggtgatcg tcttcttgtc ctgttcgtag ccgctgtagg tgaggctcac
     3901 gccgttcagg caatattgtt cgagatgatc gcggcaatgc tcgcagaccc ggcaggaatc
     3961 gaccaggcag ccgatgccga ccttgtcgcc gggtttaaac ttcgtgacgt ggctgccgac
     4021 gcgcgtgacg atgccggcga tctcgtgacc cggcaccatg gggaagatgc cgccgcccca
     4081 ttcgtcgcgc acctgatgaa cgtcggtgtg gcagataccg caatatttga tctcgatgac
     4141 gacatcgtgc tccttgggtt cgcgccgttc gaacgaatag ggcgcgagct gagacttggc
     4201 ggcgggagcg gcataagcca gggtcttgag catggcgaat cctttcgaga agcggcggag
     4261 cggctgaagt ttggtagttc gggaattacg aactattgaa gcttgagtca agtcggaata
     4321 gcgtgacaat catgaaaccg ctgtcgcacc cgcatatcga cgacgtcacg gtggaaggca
     4381 tcctccatgc gttctccagc cccatccgca tccagatcta cgcccaactg gtggcggcag
     4441 attgtccgca atgctgctcg gcctatttgc ggatcaaagg cggcaagctg ccgaaatcga
     4501 ccttgtcgca gcatttcgcg gtcctgaggg aagccggcct catccgcagc gagcgcaaag
     4561 gcaacgaact tcaaaacacg acgcggtgcg acgagctgga aaagcggttc ggcgccatga
     4621 tcgatgccgt tctcgacgcg tatctggcgc agagaacggc gcgcaagaaa aagcgccggc
     4681 ggcgatcgtc gctcaccccc ccttgaaaac gggatcgcgc ttttcgaaaa aggcggcgag
     4741 cgcctcggcg tgatcctcgc tcgcgtgcgc ctgcgcctga aacgccgcgg acctttccaa
     4801 aatggtttcg aggtccgccc ccagggcttc gcgcatcagc ttcttggtca tgcgcagaac
     4861 gccgggcggc tggcggcaga tttccgccgc aaggccccgc gcttcgttca tcagcatttc
     4921 gggcggcacg gtcctggaaa ccagacccca tttcagcgcc gtctcggcgt cgatggtgtc
     4981 ggcgatgaag aaaagttcgg ccgcgtgcgc ctgaccgata acgcgcggca gaatccaggc
     5041 gccgccgtcg cccggcacca ggccgatctt gaggaagggg gtgccgaaaa ccgcgaacct
     5101 ggaggcgatg cggatgtcgg caaggcaggc gatatcgttg cccagcccga tggccggacc
     5161 gttcaccgcc gcgatcagcg gcacttccag cccccacagg ctgcggacga tctggtggat
     5221 ctcgcgcttg tagctctcgc ggacctgatc cggggtcccc gcgaagatgc cgctcttctc
     5281 gcgcatcgcc ttgacgttgc cgccggccga gaacgccgtg cccgcgccgg tcagaatggc
     5341 acagcgcaat tcgcggtcgg cattgatttt cgcggcggcc gcggcgaaca gctcaccgtc
     5401 gccctcctcg cccagcgcgt tgcggctctc gggccggtta atggtaagcg tggcgacgaa
     5461 gccgtgtttc tcgtacagca gcacagacat gcgttccctg cttcgccggg ggtgacagcg
     5521 cagtctagaa catgatgccc tcggagagaa ccagctacat tagctgcatt acgcgcttgc
     5581 gcgggccggt ctgagtagat tccgagcctt tctggccgaa ttgggggatg acaatatcct
     5641 cagatcgctg ccaacccgtg cgcctccggc gtataccgca gcatggaagg aatcacacat
     5701 gcatatgttc ttgaaaggcg cggcgatcgc gctggcgctg gccggcgcaa cattcgcgac
     5761 cgccggcacg tccaatgccg cgggcgtttc cttcggcata acagacaatg gccgccacaa
     5821 cagcagcacc gccatgtcgt tcaattttgg cgaagtcgca ttcgcgtacc aggacggcta
     5881 ttgggacaac aatcgccgct ggcacaagtg gcgcaaccgt cgcgaccacg ataattatcg
     5941 caagcaatac agcaacaagt accgcaacgg gaatcatacg cgctactccg gccaaggctg
     6001 gcagcggcgc taagttcgcc tcaatcgaag tccccgaccg catcggcccc ctgttgctcg
     6061 tcggggactt cggctttccg cggcggcttc cgtaatccaa actgtcccat ccgtgttcga
     6121 ttaccgtccg atcggttcaa cagccgccgg caagttgaac gatttggcag gaacaccttt
     6181 atgcttattc aagtgaatac ggacgacaat gtcgaaggcc gcgagaaatt cgttctctat
     6241 gtcgaggcgg aagccgcccg catcttaagc cggttcagcg accagattac ccggctggaa
     6301 atccatctca gcgacgaaaa cggcgataaa tccggcagca cagacaagcg gtgcctcatg
     6361 gaggctcgtc ccgccggacg cccgcccgtg gcggtcagcc acgacggcgc gagcttggag
     6421 gatgcctgga gcggggccgc caagaagatg ctgcggctgc tcgaatccac cctgaggcga
     6481 gtgagcgatc gcaagggcgg ggcgtcgata cggacggacg gcatcgctga gtgatcgagc
     6541 cagccggaac gcaaccgtcc ggtccttcgg gttgacgcag aaaacattcc gcgcttttcg
     6601 ccgcccgtcg cggacactat agtgttgttc cgtcgacgga cagatcggcc ggcaattctg
     6661 ggaatgccaa tgaattctga ttcaatgatg acgcctccat ttccgtctgc gctggaggag
     6721 caagacgctt atatttcgcc ggaagaggtc gacagcccga ttctgaagca cggtttcgcc
     6781 tattggaagg atttgcgcgc aggacgggaa tttcccgcgc gcagcgacat cacgccgcgc
     6841 gggctgggaa gcctgctgcg caacgccttg ttgttgcgcg tgatcgaccg aggcgaggac
     6901 tacgagtacc gcatcgtcgg agacgcgcat gtgctggcgc acggcgtcag cgttcagggc
     6961 aagctgtggt cgcaggtcaa caattcgaaa ag
//
LOCUS       DASWPU010000651         4875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_91615,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000651 DASWPU010000000
VERSION     DASWPU010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91615"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1056)
                     /locus_tag="VGC27_13900"
     CDS             complement(40..1056)
                     /locus_tag="VGC27_13900"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY0283702.1"
                     /translation="MFQKNWQELIKPQKLRVDAGQDAGRSATIVAEPLERGFGLTLGN
                     ALRRVLLSSLQGAAVTSIQIDGVLHEFSSIAGVREDVTDIVLNVKQIALRMHAEGPKR
                     LSLKATGPGEITAGQIQAVADVEILNPDLVLCTLDEKVDFRMELTVATGKGYVPADRN
                     RPEDAPIGLIPVDSLFSPAKKVSYKVENTREGQILDYDKLTLTVETNGAIAPDNAVAY
                     AARILQDQLQVFINFEEPRERVAEEAKPDLAFNPVLLKKVDELELSVRSANCLKNDNI
                     VYIGDLIQKTEAEMLRTPNFGRKSLNEIKAVLAEMGLHLGMEVPGWPPENIEDLAKRY
                     EDQY"
     gene            complement(1189..1590)
                     /gene="rpsK"
                     /locus_tag="VGC27_13905"
     CDS             complement(1189..1590)
                     /gene="rpsK"
                     /locus_tag="VGC27_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY0283703.1"
                     /translation="MAAPADKKAPRARKKEKKNVVVGVAHVAATFNNTIITITDTAGN
                     AVSWSSAGMMGFKGSRKSTPYAAQVAAEDAGKKAVDHGMRTLEVEVSGPGSGRESALR
                     ALQTVGLVVTSIRDVTPIPHNGCRPPKRRRV"
     gene            complement(1591..1968)
                     /gene="rpsM"
                     /locus_tag="VGC27_13910"
     CDS             complement(1591..1968)
                     /gene="rpsM"
                     /locus_tag="VGC27_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY0283704.1"
                     /translation="MARIAGVNIPTQKRVLIGLRYIHGIGPAKAKEICDKVGIAPERR
                     VHDLTDAEVIQIRETIDRDYLVEGDLRREVSMNIKRLMDLGCYRGLRHRKSLPVRGQR
                     THTNARTRKGPAKAIAGKKKVTK"
     gene            complement(2172..2768)
                     /locus_tag="VGC27_13915"
     CDS             complement(2172..2768)
                     /locus_tag="VGC27_13915"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004970717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY0283705.1"
                     /translation="MNLVLFGPPGSGKGTQAKILQERHKWPQLSTGDMLRAAIAAGTE
                     LGKKCKAIMDSGELVPDEVVVGIIAERYDQPDCSEGAIFDGFPRTIPQAEALDAMLKG
                     RGRKIDMVIELKVDDAVLIGRVEQRIKQSGGVARADDTPETLKNRLEVYYKNTAPLIE
                     FYKKQGKLVTIDGMAPIEEVTKAIAEVLDRAGRKGCLG"
     gene            complement(2765..4090)
                     /gene="secY"
                     /locus_tag="VGC27_13920"
     CDS             complement(2765..4090)
                     /gene="secY"
                     /locus_tag="VGC27_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY0283706.1"
                     /translation="MPSAAEQLAANFNFSSFAKAKELRQRLLFTLGALIVARLGTYIP
                     MPGIDPAGMANFINTQGGGLLDVFNTLAGGAVQRMAIFALGIMPYISASIIMQLMTAV
                     FPELEALKKEGAAGQKVINQYTRYGTVALAAIQAFGIAVGLEQGQGIVLEPGMFFRMT
                     AVITLTGGTILLMWIGEQITSRGVGNGISLIIFAGIVARFPQIAGQSLESARTGATNP
                     LMLIIFMVAAVALVSFIVFMERAQRRLLVQYPKRQVGQRMFGGESSHLPLKLNVSGVI
                     PPIFASSILLMPMTVASFNAQNIPDWLQTVVIYLSRGHPVYLLIYGAFVLFFAFFYTS
                     IVFNPGETAENLKKYGGFIPGIRPGKKTADYIDFVLTRCTTVGAIYLAVVCLIPEYFQ
                     SDVSFALGGTSILIVVSVTMDTVAQIQSHLVAQQYEGLIKKAKLRGARR"
     gene            complement(4278..>4875)
                     /gene="rplO"
                     /locus_tag="VGC27_13925"
     CDS             complement(4278..>4875)
                     /gene="rplO"
                     /locus_tag="VGC27_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY0283707.1"
                     /translation="GRVAAFGLPGHGDVKMKLNQIANNPGAHKHKHKVGRGSSSGLGK
                     TSGRGVKGAKARTGTKVYGFEGGQMPLHMRMPKRGFNNVFAKDFAEVNLGRIQKAIDD
                     KRLDASGKIDLEVLKKAGLIAKNRDGVRLLAKGEIKAKLEIEVAGASASAVEAVSKAG
                     GSVVTTFKKKAYMNKKGTPGKRVQRRVKSAEKRAARGG"
BASE COUNT          905 a         1531 c         1427 g         1012 t
ORIGIN      
        1 gagcttacgg ccttggttac gatgacgcat gtgagtcccc tagtactgat cctcgtaacg
       61 cttcgcgagg tcttcgatgt tctccggcgg ccagcccggc acttccatgc cgagatgcag
      121 acccatttcc gcgagcaccg ccttgatctc gttcagcgac ttgcggccga agttcggtgt
      181 acggagcatc tccgcttccg tcttctggat cagatcgccg atgtagacga tgttgtcgtt
      241 cttcaggcag ttcgccgaac gcaccgacag ctcgagttcg tcgaccttct tgagcagcac
      301 cggattgaag gcgagatcgg gcttggcctc ttccgcgacg cgctcgcgcg gctcctcgaa
      361 attgatgaag acctgaagct ggtcctgaag gatgcgcgcg gcataggcga cggcgttgtc
      421 gggcgcgatg gcgccgttgg tttccaccgt cagcgtcagc ttgtcatagt cgagaatctg
      481 gccctcgcgg gtgttctcga ccttgtaaga gactttcttg gccggggaga acaggctgtc
      541 gaccgggatc aggccgatcg gcgcgtcctc cggacggttg cggtcggcgg ggacgtagcc
      601 cttgccggtg gcgaccgtca gctccatgcg gaagtccacc ttctcgtcca gggtgcagag
      661 cacgaggtcg ggattgagga tttccacgtc ggcgacggcc tgaatctggc ccgccgtgat
      721 ttcaccgggg ccggtcgcct tcagcgacag ccgcttgggt ccttcggcat gcatgcgcag
      781 cgcgatctgc ttcacgttga gcacgatgtc ggtgacgtcc tcgcgcacgc ccgcgatcga
      841 agagaattcg tggagcacgc catcgatctg gatcgaggtc accgccgcgc cttgcagcga
      901 cgacagcagc acgcggcgca gcgcgttgcc gagcgtaagc ccgaatccgc gttccagcgg
      961 ctccgcgacg atcgtcgcgg aacggccggc atcctgcccc gcatcgacgc ggagcttttg
     1021 cggcttgatc agttcctgcc aattcttctg aaacatagtg cctcatcgtt tcccggacgt
     1081 gtcggaccac gcccatttcg ttcgacggga agattcttcc gaactaaact ctctttcggt
     1141 cgcacggccc cacggaaaac cgcttcacac ttttcctggc cgatgctcct aaaccctgcg
     1201 gcgcttgggc ggccggcaac cattgtgcgg gatgggggtc acatcgcgga tcgacgtgac
     1261 caccagaccg acggtttgca gcgcgcgcaa ggcggattcg cgacccgaac ccggaccgct
     1321 cacttccacc tccagcgtac gcatgccgtg atcgacggcc ttctttcccg catcctcggc
     1381 ggcgacctgt gcggcatagg gcgtcgactt gcgcgagcct ttgaagccca tcatgccggc
     1441 cgacgaccag gatacggcgt tgccggcggt atccgtgatc gtgatgatcg tgttgttgaa
     1501 ggtcgcggcg acatgcgcca cgccgacgac gacgttcttc ttttccttct tgcgggcgcg
     1561 aggcgctttc ttgtcggcgg gagcagccat ttacttcgtc accttcttct tgccggcaat
     1621 cgccttggcg gggcctttgc gcgtgcgcgc attggtatgg gtacgctgcc cgcggacggg
     1681 caggctcttg cggtgacgca ggccgcgata acagccgaga tccatcagcc gcttgatgtt
     1741 catcgacact tcgcggcgca ggtcgccttc gacgagataa tcgcggtcga tcgtctcgcg
     1801 aatctggatg acttccgcgt cggtcaggtc atggacccgg cgttccgggg cgatgcccac
     1861 tttgtcgcag atctccttgg ccttggccgg gccgatcccg tggatgtagc gcaggccaat
     1921 aaggacgcgc ttctgggtag gaatattgac gccggcgatg cgtgccacga gcttctccga
     1981 atgcctttaa ggcgttgaat taactacatt attctcttac gtacgacccc ggcaaacccc
     2041 atccgccact ggcagaaaag ccaatcacgc cggaattttc ctggaaaatc ggccgcaagg
     2101 gcgcggatta tattcgtgcg cacacctcag tcaactggcc aactccaagg attccccaag
     2161 gtctaccaag tctatcccag gcatcccttc cgtcctgccc tgtccagaac ctcagcgatg
     2221 gccttggtaa cctcctcgat cggcgccata ccgtcgatcg tcacaagctt cccctgcttt
     2281 ttgtagaatt cgatcaaagg cgccgtgttc ttgtaataga cctccagccg gtttttgagg
     2341 gtttccggcg tatcgtcggc gcgggcgacg cccccgctct gcttgatgcg ctgttcgacc
     2401 cggccgatca gcacggcatc gtccaccttc agttcgatca ccatgtcgat cttccggccg
     2461 cggcccttca gcatcgcgtc cagagcctcg gcctgcggaa tggtgcgggg gaaaccgtcg
     2521 aaaatggcgc cctccgagca gtccggctgg tcgtagcgtt cggcaatgat gccgaccacg
     2581 acttcgtccg gcacaagctc gccggaatcc atgatcgctt tgcacttctt gccaagctcg
     2641 gttccggcgg cgatggccgc acgcaacatg tcgcccgtgg atagctgcgg ccacttgtgg
     2701 cgttcctgca gaatctttgc ctgcgtgcct ttgcctgaac ccggcggtcc aaaaagaaca
     2761 agattcaacg tcgtgctccc cgaagcttcg ccttcttgat caatccctca tactgctggg
     2821 cgaccagatg actctggatt tgcgccacag tgtccatggt cacgctgacc acaataagga
     2881 tcgacgttcc gccaagcgcg aacgagacat cgctctgaaa gtactccgga atcagacaca
     2941 ccaccgccag atagatggcg ccgaccgtgg tgcagcgcgt aagaacgaaa tcgatgtagt
     3001 cggcggtttt ctttccgggc cggatgccag ggatgaagcc tccgtatttc ttcaggttct
     3061 cggcggtctc tcccggattg aaaacgatgg aggtgtagaa gaacgcgaag aacaagacaa
     3121 acgcgccgta gatgagaaga tacaccggat gaccgcgact gagatagatg acgacggttt
     3181 gcagccaatc gggaatgttc tgcgcgttga acgaggcaac cgtcatcggc atcagaagga
     3241 tggacgacgc gaaaatcggc gggatcacgc ccgagacgtt gagcttgagc ggcaaatgcg
     3301 aggattcccc gccgaacatc cgttggccga cttgccgctt gggatactgc accagaagcc
     3361 gccgttgggc gcgctccatg aagacgatga acgacacaag cgcgaccgcc gcgaccatga
     3421 agataatcag catcaacgga ttggtcgcgc ccgtccgcgc cgactccaga gactggcccg
     3481 cgatctgcgg aaaccgggcc acgatgcccg cgaaaatgat cagggaaatt ccgttaccga
     3541 cgccgcggct ggtaatctgc tcgccgatcc acattaaaag gatcgtgccg cccgtgagcg
     3601 ttatcaccgc ggtcatgcgg aagaacatgc cgggctccag gacgatcccc tggccctgtt
     3661 ccaggccgac ggcgatgccg aacgcctgga tcgccgccaa ggcgaccgta ccgtaacgcg
     3721 tatattggtt gatgaccttc tgcccggctg cgccttcctt cttcagcgct tccaactcgg
     3781 gaaacaccgc cgtcatcaac tgcatgataa tcgaggcgga aatgtaaggc atgatgccga
     3841 gcgcgaagat cgccatgcgc tgcacggcgc cgcccgccag ggtgttgaag acgtcgagga
     3901 gaccgccccc ttgcgtgttg ataaaattgg ccatgcccgc aggatcgatg cccggcatcg
     3961 ggatgtaggt gccaagccgc gccacgatca acgcgccaag agtgaacaag agacgctgcc
     4021 gtagttcctt cgccttggcg aaagagctga aattaaaatt tgctgccagt tgttcggctg
     4081 ctgatggcat cttctactcc cgactcgcgc cgactttata tcgggccggc ctctcgcttc
     4141 gacccgcctg cgctgaagca acggcaggca agcgcaatat aatcagcccg ccggagcatt
     4201 gcgaaggcgg acaagcccag ggcgaggaga tgtttagact gcgtgccgag cttgccgaag
     4261 cacgcaggcg gattggtcta tccacctctt gcggcccgct tttccgccga tttaacacga
     4321 cgctgcaccc tcttcccagg cgtacccttc ttgttcatgt aggccttctt cttgaaggtc
     4381 gtgacaaccg agccgcccgc cttgctcacc gcttcgacgg cactcgccga cgcacccgca
     4441 acttcaattt caagcttagc cttgatttcg ccttttgcca gcagacgcac gccatcgcgg
     4501 ttcttggcga taaggccggc ttttttcagc acttccaagt cgatcttgcc ggaggcgtct
     4561 agccgcttat cgtcgatcgc tttctggatc cggccgagat tcacttccgc gaagtctttg
     4621 gcgaagacgt tattgaagcc gcgcttcggc atgcgcatgt gcagcggcat ctggccgcct
     4681 tcgaagccat agaccttcgt accggtgcgt gccttggcgc ccttgacgcc gcggccggag
     4741 gtcttgccca ggcccgagga ggagccacgg ccgaccttgt gcttgtgctt gtgggcgccg
     4801 ggattgttgg cgatctggtt gagcttcatt ttaacatctc catggccggg caggccgaag
     4861 gccgcgaccc ggcca
//
LOCUS       DASWPU010000652         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_79181,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000652 DASWPU010000000
VERSION     DASWPU010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79181"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2808)
                     /locus_tag="VGC27_13930"
     CDS             complement(<1..>2808)
                     /locus_tag="VGC27_13930"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0283708.1"
                     /translation="YRSTSFSTWAIRNPIPPIVLFLVLTVSGLVAYWQLPVNNMPAIV
                     IPVVTVQIVQPGAAAPEIETQITRIVESSLAGMQGVKHITSSISEGASLTTIEFTEET
                     DFDRAVNDARDAVAKVRDRLPQSILEPQVQRVDVDGGAILTYSVLAPEMRAEDLSWFV
                     DDTLNRELLSVTGVARVQRQGGVDQEITLTLDPAKLMALGISAAEISRALAQMNVDRP
                     GGRLTISGTEYTVRTLGSAKSLAALRDMRVPLSGGHDVRLADLGTLSDSSAEQRSIAR
                     LDGEIAVTFQVFRTKGSSEVTVARAVEKKLAEIRRTHPGIAFRPIFSLATSTEISFEA
                     TMANFAEGAVLTIFVVFLFLKDKRATVIAALAIPLSIIPTFLFMYAIGFTLNSVTLLA
                     ISLVTGVLVDDAIVEIENIHRHMREGRSPYAAAMFAADEIGLAVIATTAVICAVFLPV
                     SFMSGIAGQFFKQFGLTVAVAAFFSLIVARLITPMLAAYLLKTPERREEKPSRWKDAY
                     HRLIAWTLDHRLKTMGLAAASLAASFGLIPLIPGGFIPYEDVGQSRLTMDLPRGATLA
                     ETDAAAQEVMQILKRHKEVEYVLTSIGGGVVASDGPQMGEGESGGVNKAEIDIKLVPR
                     GKRALDQRAFERKVQSELTALPDRRIAFANSGGEKDISIALTGDDGAALERTAAAVEK
                     QMRTIPELAGVSTTASLKQPEIVVTPDFAKAAKLGVSVAAISDAINVGTIGYLEANLA
                     KFNVGDRQIPIRVRLRSQNQHLSTLENLKLPTAAGASVPLSAIAAITYASGPTTIERY
                     DHQRKIALEANLNGVVIGDALKKIDALPAMKEMPAGVRVQNSGDAEKMGELFSGFAKA
                     IGAGLLMVYAVQVLLYKDWIQPLTRMAALPLSIGGAFVLMLLTGTELSMPALIGILML
                     MGIADKNAILLVDYMLERIRQ"
BASE COUNT          561 a          856 c          834 g          557 t
ORIGIN      
        1 cctggcgaat acgctcgagc atatagtcca ccagcaggat tgcgtttttg tcggcaatgc
       61 ccatcagcat cagaatgccg atcagggccg gcatgctgag ctcggtgccg gtcagcagca
      121 tcaagacgaa cgcgccgcca atcgacagag gcaatgccgc catgcgggta agcggctgga
      181 tccaatcctt gtatagcaac acctgcaccg catagaccat caagaggccc gcgccgatgg
      241 ccttggcaaa tccggaaaag agttcgccca tcttctcggc atcgccggaa ttctgcaccc
      301 gcactccggc cggcatctcc ttcattgccg gtaacgcgtc gatcttcttg agcgcatcgc
      361 cgatcaccac tccattgaga ttggcttcaa gggcgatctt gcgctgatga tcatagcgct
      421 cgatggtggt ggggccgctg gcataagtta tggccgctat cgccgaaagc ggaacactgg
      481 cgccagcggc ggtgggcagc ttcaggtttt ccaatgtgct caaatgctgg ttttggcttc
      541 gcaggcgcac gcggatgggg atttgccggt cgccaacatt gaacttggcg agattggcct
      601 cgagataccc gatggttccg acgttgatcg cgtcgctgat cgccgcaaca ctgacgccca
      661 gttttgctgc cttggcaaaa tcgggagtga cgacaatctc cggctgtttg aggctcgctg
      721 tggtgctgac acccgcaagc tccggtattg tccgcatttg cttctcgacg gctgcggccg
      781 tccgttccaa cgctgccccg tcatccccgg tcagagcgat ggagatatcc ttttcaccac
      841 cggagttcgc aaaggcaatg cgcctgtcgg gcaatgccgt cagttcggat tgcacctttc
      901 gttcgaaagc gcgttgatcg agggcgcgct ttcctcgggg aaccagcttt atatcgatct
      961 ccgctttgtt cacgccgcca gactcgcctt cgcccatttg cgggccgtcg gacgccacca
     1021 ccccgccgcc gatgctggta aggacatatt cgacttcctt gtggcgtttg agtatctgca
     1081 tcacctcctg ggcggcggcg tctgtctcgg cgagggtggc tccgcgcggc aggtccatcg
     1141 tgaggcggga ctgaccaaca tcttcatagg ggatgaagcc gcccgggatc agcggtatca
     1201 atccaaagga ggccgccaaa ctggccgccg cgagacccat cgtctttagg cgatgatcca
     1261 gcgtccaggc gatcagccgg tgatacgcgt ctttccagcg cgatggtttt tcctcccggc
     1321 gttcgggggt cttcaacaga taagcggcca gcatgggcgt gatgaggcgt gccacgatca
     1381 acgagaaaaa cgcggccact gcgaccgtca ggccaaattg cttgaagaac tgtccggcga
     1441 tcccactcat gaagctcacc ggcaggaaga cggcgcagat cacagccgtc gtggcgatga
     1501 cggcaaggcc gatctcatcg gcggcaaaca tcgctgccgc atagggcgat cggccctccc
     1561 gcatgtggcg atggatgttc tcgatctcca cgatggcatc atccaccagc acgcccgtga
     1621 ccagagagat cgcgagcagg gtcacgctgt tgagcgtaaa gcctatggcg tacatgaaca
     1681 aaaatgtcgg aatgatggaa agcgggatgg ccaacgcggc aatcacggtg gcgcgtttgt
     1741 ccttaagaaa caagaacacc acaaagatgg tcaacaccgc gccttcggcg aaattggcca
     1801 tggtggcctc gaagctgatc tccgtcgatg tggcgagcga aaagatcgga cgaaaggcaa
     1861 tgcctggatg cgtccgccgt atctccgcca acttcttttc gacagcccgt gcgaccgtca
     1921 cctcgctgct gcccttggtc cggaacacct gaaaggtgac ggcaatctcg ccgtccaacc
     1981 gcgcaatgga acgctgttcc gcactgctat cgctgagcgt tcccaaatcg gccaaccgca
     2041 cgtcatgacc gccgctcagc ggaacacgca tgtcccgcaa tgctgcaagt gatttggcac
     2101 tgcccagggt gcgcacggta tattcggttc cggaaatcgt taggcgcccg ccggggcgat
     2161 cgacattcat ctgcgccagc gcgcggctga tctcggcggc cgaaatccca agcgccatca
     2221 gcttggcggg atcgagcgtg agcgtgattt cctggtctac gccgccctgc cgctgaaccc
     2281 gagcgacacc agtgacactc agtagctctc ggttcagcgt gtcatctaca aaccagctca
     2341 aatcttccgc gcgcatctct ggcgccaaga cgctgtaggt caggatcgca ccgccatcga
     2401 cgtcgacgcg ttgcacctgc ggctcgagaa tggactgcgg cagccggtca cgaaccttgg
     2461 ctaccgcgtc gcgggcatcg ttcacggcgc ggtcgaagtc cgtctcctct gtgaattcga
     2521 tggtggtcag cgacgctccc tccgaaatgg atgaggtgat gtgcttgacg ccctgcatcc
     2581 cggcgagcga gctttcgacg atgcgcgtga tctgggtttc gatctccgga gctgcggcgc
     2641 cgggctggac aatttgtacg gtcacgaccg gaatgacgat ggccggcatg ttattgacgg
     2701 gcaactgcca gtaagcgacc aatcccgata cggtcagcac cagaaacagc acgatgggcg
     2761 gaatgggatt gcggatcgcc caggtcgaga acgacgtgct gcggtagt
//
LOCUS       DASWPU010000653        11447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_5768, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000653 DASWPU010000000
VERSION     DASWPU010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11447
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5768"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VGC27_13935"
     CDS             complement(<1..910)
                     /locus_tag="VGC27_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY0283709.1"
                     /translation="MHKVLALAPDWLIATLVLILTIAAAAALFNLLRVILHAALKSEQ
                     KLLQAIVQRAGRLAQFAFILLAAALVVPALPLDRGATDILHRILIAAFIVLLGWIAVL
                     ASDLAIERYVGRFKLDVEDNLMARKTITQMRMLKRIGEVLIGLVTAAFALMTFDTVRE
                     YGISLFASAGIAGLAVGLAARPLLGNLIAGVQLAITQPIRLDDAVVVEGEWGRIEEFT
                     STYVVIRLWDSRRLIVPLSYFLEHPFQNWTRTSSSILGSAIFHLDYTADIGRIRARFE
                     EIVKAAKQWDGRVANMQVTDISERSIM"
     gene            complement(912..2327)
                     /locus_tag="VGC27_13940"
     CDS             complement(912..2327)
                     /locus_tag="VGC27_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY0283710.1"
                     /translation="MARIVIVSNRVPVPNSRGPQAGGLAIVLKDAVKPGTLWFGWSGR
                     TAAETSTDAHIVTHNSVDYATIDLSQDAYKKFYIGFANGTLWPLLHFRLGLMEFRREH
                     LEEYRAANELFASALVKLLRPDDLIWIHDYHLIPLANALRKLGMTNRMGFYLHIPFVP
                     SSVFGALPQGEELLKEFCTYDVVGFQTEEYRRNFLDCTRQVLHYPIDGGGNISAPGGN
                     VRSIVAPVGIGGTTFHKQAERSATSRNARRLLDSLVGRHLMIGVDRLDYSKGIPNRFE
                     AFNRLLSRFPEHRMNVSFLQIAAPSREDVKEYAALRPVLNRMAGDINGRHGAFDWVPL
                     RYISQGLARSTLAGFYRLARIGVVTPLRDGMNLVAHEYVAAQNPEDPGVLVLSRFAGV
                     ANYFDDALIVNPFDPDEIAEAMHEALIMPLEERRRRHRKLLDRLSVLTAEAYCATFLE
                     ALAGKDGHKTPALRSEEDVIL"
     gene            complement(2312..2713)
                     /locus_tag="VGC27_13945"
     CDS             complement(2312..2713)
                     /locus_tag="VGC27_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283711.1"
                     /translation="MSHLNLAPIGNCAVAGLIDARACHVWFCFQRLDADPVFNALASG
                     QPHITLRMRPTFGNGSAAPQVSIGSNHARFIGSENVLGVTTDTAVSERSHYLHPLYPI
                     VSRTERKEYEAPDLAVFCGMCSWEHGLWHAS"
     gene            complement(2722..3495)
                     /gene="otsB"
                     /locus_tag="VGC27_13950"
     CDS             complement(2722..3495)
                     /gene="otsB"
                     /locus_tag="VGC27_13950"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY0283712.1"
                     /translation="MTVVDTGRMPNIELTQSDFEVPKPCQNWALFLDIDGTLIDIAAG
                     PDAVIVPEALAPTLASASDWLGGALAIVSGRPFGKIDELMAPLKLPCAGEHGAVLRLP
                     DGTFRNAKAECAVPEAWKSHVRAATQGWSGVVVEDKPYSIAAHFRRVPERERDVHDLL
                     ESVVAGNSQGFEVLSAHMAFEVRHHKLTKAAAVHEFMKHAPFCDRIPVFVGDDVTDED
                     GFRAAQELGGIALHVHGAFKGRTSNVRRWLEAFRSADIG"
     gene            complement(3581..4585)
                     /locus_tag="VGC27_13955"
     CDS             complement(3581..4585)
                     /locus_tag="VGC27_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEY0283713.1"
                     /translation="MKIVVFEAEDQERGAFDRLLPAHEVVFVTDPLKASNVEEYADAD
                     VVSTFIYSDLSLNIIEQLPRLKLIATRSTGYEHINIEECKRREIAVSNVPTYGANTVA
                     EHIFALLLAISHRLPEAIERAQRGHFSPLGLQGFDLAGKVLGVIGTGSIGRHVIKIAK
                     GFDMEVAAYDVRPDTKLAETLGFHYMSLDELLARADIISLHVPSSPENHHILNTKTFA
                     SMKTGVVIINTSRGGLISTSGLIQALTSGKVAAAGLDVLPAEPMIREEAELIRSIFNK
                     EHDLRNLVATHVLLRMRNVIITPHSAFNTREAVGRIVETTADNIAAFLDGHPKNIVGG
                     "
     gene            4895..6205
                     /locus_tag="VGC27_13960"
     CDS             4895..6205
                     /locus_tag="VGC27_13960"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY0283714.1"
                     /translation="MQALFRLWDFDRILPETAGTLLTRIETPVILIQLGAIVAAGAAA
                     FWLSMLLRPRLTELTLHLLPRNWTPTFVKVAASVAPPVIWLLLLWPVTVIGTLLGYAL
                     PLASAGVGLLVAWVCIRLLSFSVKSPLVSATISVVVWSIAALSILGLLDPLMHELDAS
                     AIRLGKYRVSALTAINALFVLAALLWLTTILFRFLQRQITHMTSLTPSLQVLLTQLLQ
                     ILLPAIAVIVALSYAGIDLTVLTVVFGAVGLGIGLGLQRVVGNLVAGLTLLMGKSIKP
                     GDIIEYKNGYGWVSAMGARYVTLRTRDGVEHLVPNDYFLENGVENWSYSDVRLRLRAA
                     VGIAYDSDVHRAIALCIEAAKSVKRVLEYPEPLCLLKGFGNNSIDLEIRMWIEDPRNG
                     TKNVISEVLLAVWDRFKEAGISFPLPPRDVHIVSKPRESGTPLS"
     gene            complement(6354..6920)
                     /locus_tag="VGC27_13965"
     CDS             complement(6354..6920)
                     /locus_tag="VGC27_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEY0283715.1"
                     /translation="METLLISAVVVALAEIGDKTQLLAIVLTARFKKPVPIILGIFCA
                     TIANHILAATGGYFLSDLLSGAWFQYVVAASFIAMALWTLFPDRLDTKEKDHALAGAF
                     ITALIAFFLVEIGDKTQVATAALAARFHSVFLIAAGTTMGMLLADIPAVLLAERAMNF
                     VPLKYVRTGAAFLFFVLGVWGLIATWTR"
     gene            complement(7094..10681)
                     /locus_tag="VGC27_13970"
     CDS             complement(7094..10681)
                     /locus_tag="VGC27_13970"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0283716.1"
                     /translation="MFRKIAAFELRYQLTSPVFWVAFGIFFLLTFGATTNSQIQIGSA
                     GNVFVNSPYSIIQKLAIMSVFAIFILTAFVANVVVRDDETGYGPIIRATPVGKFDYLF
                     GRFTGAFAAGMIAFASVPLGILIGSWMPWLDPEKLGPFRVEYYLYAYFLVAMPTLLVM
                     GAMFFAIATATRSMLLTYVGVVGFLVAYLVLIGLFNQPQYDHLIGLIEPFGLGAVGEV
                     TKYWTTFDRNTMLPPLNGVIAENRLIWFGASFAMLALAYFIFRFETKGAKRERAAGPD
                     TAPAPRKTGPLPAPRFDSYATRVQSWKWARFEMAQVFKSPAFFVLLAIGLFNAGGSMW
                     YADQFHDHTIYPVTRVMINALRGAFTIIPVIIALYYAGELVWRERDRRTHEIFDACPV
                     PDWAFMAPKMVAIALVLLATSAISVLAAVGIQTLKGYYNYELTHYLLWYVIPETLAAV
                     ELAVLSVFVQTISPNKYVGWGLMGLYLVATMTFTLVGFEDRLYLYGATPDVPLTDMNG
                     IGKFWIGAAWFDFYWCAFALALTVLTYALWRRGTETRLMPRIARLPHRLKGSAGVIMA
                     VALCAFAGSGAYNFYNTHVLNEYRTSIGDDKFSADFEKALLPFEKLPQPRITDVTLNV
                     AVYPHDARIETEGSYAIVNRTAHPIPDLHIRLDRDLKAVALNIDGATLKKDYGRFNYR
                     IYAFDKPMQPGEKRVVRFRTLLEQKGFRNSGNTTRIVDNGTFVNNTEIAPTIGMDRSA
                     SLTDPVKRRRYGLPSQLRPPKLEDDSARANNYLTHAADWVMSDITVSTVADQTPIAPG
                     YKVAESTANGRRTVRFKSDAPIIPYFSIQSADYAVKRDKWKNVDLAVYHDPRHPYNVD
                     LMIAAMKASLEVYSRVFSPYQFQQVRILEFPAYATFAQSFANTIPYAEGIGFIQNSNA
                     IKNDPEKIDLVTYVTAHEIGHQWWAHQVIGADMQGMTLLSETFAQYSAILVMEKLYGP
                     EHVRKFLKYSLDRYLRARGSEVVEELPLERVENQGYIHYDKGAVVMYRLKEAVGEEVV
                     NRALRRLIAEYAFKPAPYPASKDFVKILRQEAEPKYDQLITDLFQRITVYDLKAADVR
                     SHKRKDGKFDVTLSVEAHKYYADGKGKETETPLHEDVDVGAFLVEPGKKGFDKSKILV
                     FERLPLKSGTQVLHLVTDRAPAFAGIDPYNEWIDRNSDDNVKSVPAPGS"
     gene            complement(10683..>11447)
                     /locus_tag="VGC27_13975"
     CDS             complement(10683..>11447)
                     /locus_tag="VGC27_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0283717.1"
                     /translation="GKSTLMRCVATLQAPTSGNIRFDDIDIVKEPEKLRRVLGYLPQD
                     FGVYPRVSAYAMLDHMAVLKGIARPGERRDTVEALLQQTNLWNVRKKAIAGFSGGMRQ
                     RFGVAQALIGRPALLIVDEPTAGLDPEERNRFLNLLSEVGENVVVILSTHIVEDVSDL
                     CQRMAIIAEGRIVTVGVPSELTASLKGRIWRKTIAKAELEAARQRYAVILTRLLGGRT
                     LIHILSDDNPGEGFEPVDPGLEDVYFTSLPQKSRAA"
BASE COUNT         2214 a         3376 c         3589 g         2268 t
ORIGIN      
        1 ccatgatgct gcgttcggag atatcggtca cctgcatgtt tgccacccgg ccgtcccatt
       61 gtttcgccgc cttgacgatc tcctcgaatc tggcgcggat gcgtccgatg tcggcggtgt
      121 agtcgagatg gaagatggcg cttcccagaa tcgatgagga agttcgcgtc cagttctgga
      181 acgggtgctc caggaaatag ctgagcggca cgatcagacg gcgcgaatcc cacaggcgga
      241 tgacgacata ggtggaggtg aattcctcga tccggcccca ctcgccttct acgaccacgg
      301 cgtcgtcgag gcggatgggc tgggtgatgg cgagctgcac gccggctata aggttgccta
      361 acagaggtct cgccgccaaa ccgacggcca gtcccgcgat gccggccgat gcgaacaggc
      421 tgatgccgta ttcgcgcacg gtatcgaagg tcatcagggc gaaagccgcg gtgaccaggc
      481 cgatcaggac ttcgccgatg cgcttgagca tgcgcatttg cgtgatggtc ttgcgcgcca
      541 tcagattgtc ttcgacgtcg agcttgaagc ggccgacata acgttcgatc gccaaatcgc
      601 tggcgaggac ggctatccat cccagcagca cgatgaaagc cgcaatcagg atacggtgga
      661 ggatatccgt cgctccgcga tccagcggca gggccggcac caccagagcc gccgccagga
      721 gaatgaatgc gaattgggcg agtcttccgg cacgttgcac gatggcttgc agcaattttt
      781 gttccgactt cagcgccgcg tgcagaatga cgcgcagcaa gttgaacaac gctgccgccg
      841 ctgcgatcgt cagaatcagc accagcgtgg caatcaacca gtcaggcgcc aaggccagaa
      901 ctttgtgcat ctcagagaat gacatcttct tcactccgca gagccggcgt cttgtggccg
      961 tccttgcccg caagcgcctc caggaaggtg gcgcaatagg cttccgccgt gagcacactc
     1021 agacggtcga ggagcttgcg gtgccgccgg cgccgttctt ccagcggcat gatcaaggcc
     1081 tcgtgcatcg cttcggcgat ttcgtctgga tcgaagggat tgacgatcag cgcgtcgtcg
     1141 aaatagttcg ccacaccggc gaaacgcgac aggaccagga cgcctgggtc ctcgggattc
     1201 tgagccgcca cgtattcgtg cgccacgaga ttcatgccgt cgcgcaaagg ggtaacgacg
     1261 ccaatccgcg ccagccggta gaatcccgcc agcgtggagc gcgccaatcc ctggcttata
     1321 tagcgcagcg gcacccagtc gaaggcgccg tgccgcccgt tgatgtcacc cgccatgcga
     1381 ttgagcaccg gccgtaaggc ggcgtattct ttcacgtctt cccgcgacgg cgccgcgatc
     1441 tgcaggaagc tcacattcat gcgatgttcg ggaaagcgcg acagtaagcg gttgaacgcc
     1501 tcgaaccggt tggggatacc cttcgaataa tccagtctgt ccacgccgat catcaggtga
     1561 cggcctacca aactgtccag aagccggcgt gcattcctgc tggtggcgga gcgttccgcc
     1621 tgcttgtgga aagttgtgcc gccgataccg acgggagcga caatcgagcg gacgttgcct
     1681 cccggcgccg atatgttgcc gccgccgtcg atcgggtaat gcaggacttg ccgcgtgcaa
     1741 tccagaaaat tacggcggta ttcctcggtt tggaaaccga cgacgtcgta ggtgcagaat
     1801 tccttgagca gctcttcgcc ttgaggcaaa gccccgaaca cgctggacgg cacgaatgga
     1861 atatggaggt agaaacccat cctattggtc atccccagct tgcggagcgc gttcgccaag
     1921 ggaatgaggt gatagtcgtg tatccagatc agatcatcgg ggcgcagaag tttcaccagg
     1981 gcgctggcga ataattcgtt cgcggcacga tattcttcaa gatgctcgcg gcggaactcc
     2041 atcaggccga ggcggaaatg cagaagaggc cacaaggttc cgttggcaaa cccgatatag
     2101 aatttcttgt aagcatcctg cgagaggtcg atggtggcat agtccacgga attgtgggtg
     2161 acgatatgcg catcggtgct ggtctcggcc gccgtgcgtc cgctccatcc gaaccacagc
     2221 gttcccggtt tgacggcgtc cttgagaacg atggccagcc cgcccgcctg gggaccgcgg
     2281 ctgttgggta cggggacccg gtttgaaacg attacgatgc gtgccatagg ccgtgctccc
     2341 atgaacacat gccgcaaaaa acggccaaat ctggtgcttc gtattcctta cgttcggtgc
     2401 gcgacacgat cggataaagg ggatgcaaat aatgggagcg ttcactgacc gccgtgtccg
     2461 tcgtgacgcc caggacattt tcgctaccga tgaagcgcgc gtggttgcta ccgatgctga
     2521 cttgtggggc ggcgctgccg ttaccgaacg tcgggcgcat acgtagcgtg atgtgcggct
     2581 ggccgctggc tagggcattg aagaccggat cggcatccag ccgctgaaag cagaaccaga
     2641 catggcacgc gcgcgcatcg atcaggccgg cgacggcaca gtttccgatg ggtgcgagat
     2701 taagatgcga catgtctcgg attaccctat atcggcggaa cggaacgctt cgagccagcg
     2761 gcgcacgttc gatgtccgcc ctttgaatgc tccgtgaacg tgaagcgcta tgccgcccaa
     2821 ttcctgtgcc gcacggaaac cgtcttcgtc ggtcacgtcg tctccgacaa agaccgggat
     2881 acggtcgcag aacggcgcgt gcttcatgaa ttcatgcacg gccgcggcct tggtcagctt
     2941 gtgatgccga acctcaaacg ccatatgcgc ggagagaact tcgaacccct ggctgtttcc
     3001 ggcgacgacg gactcgagga ggtcgtgtac gtcgcgctcg cgctccggca cgcggcggaa
     3061 atgcgcggcg atgctgtacg gcttatcctc caccacgacc ccgctccacc cctgcgtagc
     3121 ggcgcgcacg tggcttttcc acgcctccgg cacggcgcac tccgccttgg cattgcggaa
     3181 cgtgccatcg ggcaggcgga ggacggcgcc atgttcgcct gcacaaggca gcttcagcgg
     3241 cgccatcagt tcatcgatct tgccgaacgg gcggccgctg acaatcgcca acgccccgcc
     3301 gagccaatcg ctggcgctcg ccagcgtcgg tgcaagagct tcagggacaa tgacggcgtc
     3361 agggccggcg gcgatatcga tcaaagtgcc atcgatatcg aggaaaagcg cccagttctg
     3421 acacggcttc gggacttcga agtccgattg agtcaactcg atattgggca tgcggccagt
     3481 gtccacaacc gtcatgccac gtcaaccaaa aacgtatcga tatgaagcat ccgccaagtt
     3541 cgcgacggct tcggcgaatt tgtccgatcg cttctggata ttatccgcct acgatgtttt
     3601 tcggatggcc gtcgaggaag gccgcgatgt tgtcggccgt cgtctcgacg atccggccga
     3661 cggcttcccg cgtattgaag gcgctgtgcg gcgtgatgat cacgttgcgc atgcgcagca
     3721 ggacgtgcgt ggcgacgaga ttgcgcagat cgtgctcttt gttgaagatc gagcggatca
     3781 gttcggcttc ctcgcgaatc atcggttcgg ccggtaatac gtcgagcccg gcggcggcaa
     3841 ccttgccgct ggtcagcgcc tgaatcaacc cgctcgtcga gatcagaccg ccgcgcgacg
     3901 tgttgattat cactacgccg gtcttcatgc tggcgaaggt tttcgtgttc aagatatgat
     3961 gattctcggg cgaggacggc acatgcaggg aaataatgtc cgcgcgggcc agcagttcgt
     4021 cgagactcat atagtggaag ccgagcgttt ccgcgagctt ggtgtcgggc ctgacatcgt
     4081 acgcagccac ttccatatcg aagcccttgg cgatcttgat gacatggcgg ccgatgctgc
     4141 cggtgccgat tacgcccagg accttgcctg ccaggtcgaa gccttgcagg ccgagaggcg
     4201 agaaatggcc gcgctgggcg cgctcgatgg cttccggcag gcggtggctg atggcgagca
     4261 acagcgcgaa gatatgttcg gccaccgtgt tggcgccgta ggttggcacg ttgctgacgg
     4321 cgatttcccg cctcttgcac tcttctatat tgatatgctc gtagccggtc gaacgcgtgg
     4381 cgatcaactt cagccgcgga agctgctcga tgatgttgag gctcaggtcg gaatagatga
     4441 aggtcgaaac gacgtcggcg tccgcatatt cttcgacgtt cgacgccttg agcggatcgg
     4501 tcacgaacac gacctcatgt gcgggcagca gacgatcgaa ggcgccacgc tcctggtctt
     4561 cggcttcgaa cacaacaatt ttcatctgtc gtctcctaaa ctgccgctcg gcatcgaagg
     4621 tggttcttgc tataagcggt atctcatata cgtatttagg gtgaaaatgt gcgtttgcaa
     4681 tacttcgttg tgcactgcga catttggact tgcaatagtc tcgccgccgg agggggttaa
     4741 acggagccgg tgagagaatc accctccaat ggtccctgca agccgcggtg accatcaata
     4801 gcggcggttg agggtttttt ggcgggcgcg cccggcggct tgaacgccac gtgcccggag
     4861 acgatgatag cgcggtcata atgggcggcg atcgatgcag gcacttttcc ggctttggga
     4921 ttttgaccgt atcttgccgg agacggccgg cacgctgctg acgcggatcg agactcccgt
     4981 catcctgatt cagcttggtg cgatcgtggc ggcgggggcg gccgccttct ggctcagcat
     5041 gctgctgcgc ccgcgcctga cagagctgac ccttcacttg ctgccgcgga actggacgcc
     5101 gactttcgtc aaggtggccg cttcggtcgc gccgcccgtg atctggctgc ttttgctctg
     5161 gccggtgaca gtgatcggga ctctgctggg ttatgctttg ccgctggcaa gcgccggggt
     5221 cggcctgttg gtcgcctggg tctgcatccg ccttttgtcc ttttcggtca aaagcccgct
     5281 ggtgtcggca acgatttccg tcgtcgtgtg gagtatcgcc gcactgagca ttctggggct
     5341 gctcgatcct ctgatgcacg agcttgacgc ctcggcgatt cgtctgggca aataccgggt
     5401 ttcggctctc accgccatca acgcgctgtt tgttctggcg gcgctgctct ggctgaccac
     5461 gatcctgttt cgcttcctgc aacggcagat cacgcacatg acgtcgctga cgccgtccct
     5521 gcaggttctg ctcacgcagc tgctgcagat cctcctgccg gccatcgcgg tgatcgtcgc
     5581 cttgtcgtat gccggcatcg atctcaccgt gctgaccgtc gtattcggcg ccgtcggcct
     5641 gggcatcggc ctgggcttgc agcgcgtggt ggggaatctc gtcgccggac ttaccctgct
     5701 gatgggcaag tcgattaagc cgggcgacat catcgaatac aagaacggct atggctgggt
     5761 atcggcgatg ggcgcgcgct atgtgacgtt gcgcacccgc gacggtgtcg agcatttggt
     5821 gcccaacgat tactttctcg agaacggggt cgagaactgg tcctattcgg atgtccgcct
     5881 caggctgcgc gccgccgtcg ggatcgccta cgactccgac gtgcaccggg caatcgcgct
     5941 ctgcattgag gcggcgaaaa gcgtgaagcg cgtcctggaa taccccgagc ctctgtgcct
     6001 gctgaaaggc ttcggcaaca attcgatcga cctggaaatc cgcatgtgga tcgaggatcc
     6061 gcgcaacggc acaaagaacg tgataagcga ggttctgctg gcggtgtggg atcgattcaa
     6121 ggaggccggc atcagttttc cgctcccgcc acgcgatgtg catatcgtct ccaagccgcg
     6181 ggagagcggg acgccgctgt cgtgagcgcc gggagttcgc cgctggaact ggcgcttggc
     6241 gggcgtcgac tctaccggtg gttctggcgg ggctcggttt tgcggcagca gacctttacg
     6301 atctctagtc tctcctgtcc cacgccgtgc tccgcacggt ttggtccgcg tgtttatctg
     6361 gtccaggtcg cgatcaatcc ccacacgccg aggacgaaga aaagaaatgc cgcgccggtg
     6421 cggacatatt tcagcggcac gaagttcata gcccgttccg ccagcagcac ggccgggata
     6481 tccgcaagaa gcattcccat tgtcgttccg gctgcgatca ggaacacgct gtggaagcgc
     6541 gccgccaggg ccgcggttgc cacttgcgtc ttgtcgccga tctcgactag gaagaaggcg
     6601 ataagggccg tgataaacgc gcccgcgagc gcatggtcct tctccttggt atccagtctg
     6661 tcgggaaaca gcgtccagag cgccatcgcg atgaaggaag ccgcgacaac atactggaac
     6721 caggcgccgg acagaaggtc ggagaggaaa tagccgcccg tcgccgccaa tatgtgattg
     6781 gcgatcgttg cgcagaaaat gccgagaatg atgggaaccg gcttcttgaa tcgcgccgtg
     6841 agaacaatgg caagcaattg cgtcttgtca ccgatctcgg caagcgcgac cacaacggcc
     6901 gagatcagca gggtttccaa ttgcgcaccg ctagccagga aaggtaccaa ttagcgggcg
     6961 gagcgcgcca ggtcaacgcg gcgagaaagg cagcgataaa ttagctgtac cggtgccgta
     7021 tgaaagcgat gcccgcagca cgccgctggc gcctccccag ccacaattca atgttctccg
     7081 catcacgcgc ctttcaactg ccgggtgcag gcacgctctt cacattgtcg tccgagttgc
     7141 ggtcgatcca ttcattgtag ggatcgatac cggcgaaggc gggtgcgcgg tcggtgacca
     7201 ggtgcagaac ctgagtgcca gatttcaaag gaagccgctc gaagacgagg attttcgatt
     7261 tgtcgaatcc cttcttgccc ggctccacca ggaaagcgcc gacatcgacg tcttcgtgca
     7321 gcggcgtctc cgtctccttg cctttgccgt cggcatagta tttgtgcgct tccacgctga
     7381 gcgtcacgtc gaatttcccg tccttgcgct tatggctgcg aacgtcggcg gctttcaggt
     7441 cgtagaccgt gatgcgctgg aacaggtcgg tgatgagctg gtcgtatttc ggctcagcct
     7501 cctggcgcag gattttcacg aagtccttgg aggcgggata aggcgcgggc ttgaaggcgt
     7561 actcggcgat cagccggcgc agggcgcggt tgaccacctc ttcgccgacg gcttccttca
     7621 accggtacat gacgacggcg cccttgtcgt aatggatata gccctggttc tcgacgcgct
     7681 cgagaggaag ctcctcgacc acttcgcttc cgcgcgcgcg caaatagcgg tcgagcgagt
     7741 atttcaggaa tttccggaca tgctcggggc cgtagagctt ttccatcacc agtatcgcgg
     7801 aatattgcgc gaaggtctcc gacagcaggg tcatgccctg catgtcggcg ccgatcacct
     7861 gatgcgccca ccattgatgg ccgatctcgt gcgcggtcac ataggtaaca aggtcgatct
     7921 tctccggatc gttcttgatt gcgttggaat tctggatgaa gccgatgcct tcggcatagg
     7981 gaatggtgtt ggcgaaggac tgcgcgaagg tcgcgtaagc gggaaattcc agaatgcgta
     8041 cctgctggaa ctggtagggg gaaaacaccc tgctgtagac ctcgagcgat gccttcatcg
     8101 ccgcaatcat caagtcgacg ttatagggat ggcgcggatc gtgataaacc gccaggtcga
     8161 cgttcttcca cttgtcgcgc ttgaccgcgt agtccgccga ttggatggag aaatagggaa
     8221 tgatcggcgc gtcggacttg aagcgcaccg tgcgccgtcc gttggccgtg ctttcggcaa
     8281 ccttgtagcc cggcgcgatg ggcgtctggt cggcgaccgt cgagaccgtg atgtccgaca
     8341 tcacccagtc cgcggcatgg gtaaggtaat tgttggcgcg cgcggaatcg tcttccagct
     8401 tcggcggacg caattgcgag ggcaggccgt agcggcggcg tttgaccgga tcggtcagag
     8461 acgcgcttct gtccatgccg atcgtaggcg cgatttcggt attgttcacg aaggtgccgt
     8521 tgtcgacgat gcgggtcgta ttgccgctgt tcctgaagcc cttctgttcc agcagcgtcc
     8581 tgaaccgcac cacgcgcttt tcgccgggct gcatcggttt gtcgaaggca tagatccggt
     8641 agttgaatct gccatagtcc ttcttgagcg ttgcgccgtc gatattcagg gcaaccgcct
     8701 tcaaatcccg gtcgagacga atgtgcaaat ccggaatcgg gtgcgcggtg cggtttacga
     8761 tcgcgtagct gccttccgtc tctatccgcg catcgtgcgg atagacggcc acgttcagcg
     8821 tcacgtcggt gatgcgcggc tgcggcagtt tctcgaaagg cagcagcgcc ttctcgaaat
     8881 cggcggagaa tttatcgtcg ccgatggagg tgcggtattc gttcaagaca tgcgtattgt
     8941 aaaaattgta cgcgccgctg ccggcgaagg cacacaaggc gaccgccatg atcacgcccg
     9001 cgctgccttt gagcctgtgc ggcaggcgcg caatacgcgg catcagccga gtctccgtac
     9061 cgcgccgcca cagcgcatag gtcaaaacgg tcagcgccag cgcgaacgcg caccagtaga
     9121 agtcgaacca agccgcgccg atccagaatt tgcctatgcc gttcatgtcg gtcaggggca
     9181 cgtccggcgt cgcaccgtag agatagagac ggtcctcgaa acccaccaac gtgaaggtca
     9241 tcgtggcgac gagatagagc cccatgagac cccagcccac atatttgttc ggcgagatgg
     9301 tctgtacgaa gaccgagagc actgccagtt cgacggccgc tagcgtctcc gggatcacgt
     9361 accacagcag gtaatgagtc agttcgtagt tgtaataccc cttgagcgtc tggatgccga
     9421 cggcggcgag caccgaaatc gcgctggtgg caagaagcac cagcgcgatc gccaccattt
     9481 tcggcgccat aaaggcccaa tccggcaccg ggcaggcgtc gaaaatctcg tgcgtgcggc
     9541 ggtcgcgctc gcgccacacg agttcgcccg cgtagtagag cgcgatgata acgggaatga
     9601 tggtgaatgc gcctcgcagc gcattgatca taacccgggt caccgggtag atcgtgtggt
     9661 cgtggaactg atccgcgtac cacatggacc cgcctgcatt gaacaggccg atcgccagca
     9721 gaacgaagaa ggccggactc ttgaagacct gcgccatctc gaaacgcgcc catttccagc
     9781 tttgcacgcg cgttgcataa gaatcgaagc gcggcgcggg cagcggaccg gtcttgcgcg
     9841 gcgcgggcgc cgtgtcgggg ccggccgcgc gctcgcgttt ggcgcccttg gtctcgaagc
     9901 ggaagataaa ataggcgagc gccagcatgg cgaacgacgc gccgaaccag atcaggcgat
     9961 tttccgcgat gacgccgttc agcggcggca gcatggtgtt gcggtcgaag gtggtccagt
    10021 attttgtgac ctcgccgacg gcgcccagtc caaacggctc gatcaggccg atcaagtgat
    10081 cgtactgcgg ctgattgaac agtccgatga gaacaaggta ggcgaccaga aacccgacga
    10141 cgccgacata agtgagcagc atagagcggg ttgccgtcgc gattgcgaag aacatcgcgc
    10201 ccatcaccag cagcgtcggc atcgccacca ggaaataggc gtagaggtag tattcgacgc
    10261 ggaacggccc cagcttctcc gggtcgagcc acggcatcca gcttccgatc aggatgccga
    10321 gcggcacgct ggcaaaggcg atcatgccgg cagcgaacgc gccggtgaag cggccgaaca
    10381 gatagtcgaa cttgccgacc ggcgtggcgc ggatgatggg gccgtagccc gtttcgtcgt
    10441 cgcgcaccac cacattggcg acgaacgcgg tcaggatgaa gatcgcgaac acgctcatga
    10501 tggcgagctt ctggatgatc gagtacggcg aattgacaaa gacgttgccg gccgagccga
    10561 tttggatctg cgaattcgtg gtcgcgccga acgtcagcag aaagaagatt ccgaacgcga
    10621 cccagaatac gggcgaggtg agctgatagc gcagctcgaa cgcggcgatt ttgcggaaca
    10681 tctcaggcgg cccggctctt ttgggggagc gaagtgaaat agacgtcttc caggccggga
    10741 tcgacgggtt cgaagccctc tcccggattg tcgtccgaca ggatgtggat gagggtccgg
    10801 ccgccgagca ggcgggtcag gatcaccgca tagcgctgcc gggcggcttc cagctccgcc
    10861 ttggcgattg tcttgcgcca gatacggccc ttcaggcttg ccgtcagctc ggacggcacg
    10921 ccgaccgtca cgatgcgccc ttcagcgatg atcgccatgc gctggcacaa atcggagacg
    10981 tcttccacga tatgcgtgga aagaatgacc acgacgttct cgcccacttc cgagagcagg
    11041 ttgaggaagc ggttgcgttc ttccgggtcg aggccggccg tcggttcgtc gacgatcagc
    11101 agcgcaggcc ggccgatcag cgcctgggcg acgccgaagc gctgccgcat accgccggag
    11161 aaacccgcaa tcgccttctt gcgcacgttc cataagttgg tctgctggag cagggcctct
    11221 acggtatcgc gccgctcgcc cggccgcgcg atgcccttca gcaccgccat atggtcgagc
    11281 atggcataag cggaaacccg cggataaacg ccgaagtcct gcggcaggta gccgagcacg
    11341 cggcgcaatt tctccggctc cttgacaatg tcgatatcgt cgaaccggat attgccgctg
    11401 gtcggggcct gtagcgttgc cacgcagcgc ataagagtgg acttgcc
//
LOCUS       DASWPU010000654         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_30057,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000654 DASWPU010000000
VERSION     DASWPU010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30057"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /gene="nadA"
                     /locus_tag="VGC27_13980"
     CDS             complement(<1..541)
                     /gene="nadA"
                     /locus_tag="VGC27_13980"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY0283718.1"
                     /translation="MQTEEALLDYTSEIARAARGLYDKVAHVVPEIEWPAFAPYVMAI
                     NALKREKNAVILAHNYMTPEIFHCVADFMGDSLQLARQAAKTKAKIIVQAGVHFMAET
                     SKILSPDKTVLIPDPEAGCSLAASITGADVRLLKQKYPGLPVVTYVNTSAEVKAESDV
                     CCTSSNAAAVVEDIAREWGT"
     gene            933..1673
                     /locus_tag="VGC27_13985"
     CDS             933..1673
                     /locus_tag="VGC27_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283719.1"
                     /translation="MTSIAPIALAIAFAGAAYAGGADLPSGTLSAGYAYGLNDGTSNI
                     YGANGTLQIPVDLDWAAKTGFEAVGGYHRPDSGGMDFWYLGGALYAGGEPGRVAANYT
                     YHNMAGQGIHAYGAGAEWFVAPNATLAVRGGGLSRDHIDGGYVGAQGTWYLIPDFALS
                     AGVDHLSVGYHETGETIQAEWLPSQTTPISIYAGYQHLDYSVASADLIFAGVKLYLNG
                     DGAATLADRQRQGTNGYIVQSPKDLDQY"
     gene            complement(1854..2813)
                     /locus_tag="VGC27_13990"
     CDS             complement(1854..2813)
                     /locus_tag="VGC27_13990"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283720.1"
                     /translation="MSPAKHVVAIGLSAAIVSVVEERPSVLIVRNPASGGDALPFGPF
                     DPLLHRTLESGLRTWVGEQTYLDLGYAEQLYTFGDRGRHELKPGEGPRVVSVGYLALT
                     RQCGKLKAPATLWRDWYRYFPWEDWRAERPALIDEAIAPALASFAKSAPDSKTAEQRR
                     DRARLCFGLDRLAWDEEKVLERYELLYEAGLVQESVRDGRASAAGLLLLGTGMIFDHR
                     RILATAIARLRGKMKYRPVVFELMPPSFTLLELQRTVEAISGIRLHKQNFRRLVEAQG
                     LVEGTGEFSTQTRGRPAELFRFRREVVRERPSPGVRLGARRAG"
     gene            complement(2810..3334)
                     /locus_tag="VGC27_13995"
     CDS             complement(2810..3334)
                     /locus_tag="VGC27_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283721.1"
                     /translation="MTMILVTPLSAVLDSIRAYHPSHLVTLLSPEDMIDTPEGIAPER
                     HLRLSLNDIADPSSGLAPPGERHVAQLIAFSRKWDAAEPMLVHCWAGVSRSMAAAFTI
                     LCDRAGPGSEYEIGQEIRARASHAQPNRLLVRLADQSLDRNGRMVRAVEAMGPGRLVD
                     EGIPVELPLTIAGR"
BASE COUNT          670 a         1088 c         1127 g          659 t
ORIGIN      
        1 ccgtgcccca ttcgcgcgcg atgtcttcca ccacggcggc ggcgttggac gaggtgcagc
       61 acacgtcgct ttcggctttc acctccgcag acgtgttcac ataggtgacg accggcaggc
      121 cgggatattt ctgcttcagc aggcgaacgt ccgcgcccgt gatcgaagcg gccagcgagc
      181 agccggcttc gggatcgggg atcagcaccg tcttgtccgg cgacaggatc ttcgaggtct
      241 ccgccatgaa atgcacgccc gcctgcacga tgatcttcgc cttggtcttg gccgcctggc
      301 gcgcgagctg gagcgagtcg cccatgaagt cggcgacgca gtggaagatt tccggcgtca
      361 tgtaattgtg ggcgaggatg acggcgttct tctcgcgctt gagcgcgttg atcgccatca
      421 cgtaaggcgc aaaggccggc cattcgatct cgggcacgac atgcgcgacc ttgtcgtaaa
      481 ggccccgtgc cgcccttgcg atttcggacg tgtagtcaag cagggcttcc tcggtctgca
      541 tctcaacgcc ttcgtctcat cgcctattaa tactcgttat gagtatatat atggggcgaa
      601 aagaccggcc ttcaaggccg gctcagactt ttatgctaca tctgagcaca aagaggtcaa
      661 gccaaatcgt gaatggtccg caggcaaatt ggcccttgcc tgtttgggca gccaggagcc
      721 ttggcggcta aatttcagtc aacaccggca aaggcgtgcc tgcggcaaaa tggaaggcgc
      781 cgtggctctc ttcaatgcgc ggcccacaac aaaaccacaa ttcaaacatg gatatgccca
      841 acaccatcac caaatagcct gccagcgatg acccgggatt tggtcagcga gcaacatcga
      901 cggaaagggc agtgataatg aaggagagat tgttgacatc cattgcgccg atcgccttgg
      961 cgatcgcgtt cgccggcgcg gcttatgccg gaggcgccga cctcccgtcc gggacgttga
     1021 gtgccggcta tgcgtacgga ttgaacgacg gcaccagcaa catctacggg gcgaacggca
     1081 cgctgcagat acctgtagac ttggactggg ccgcaaaaac gggttttgaa gcagtcggcg
     1141 gctaccacag gcccgacagt gggggcatgg atttctggta cctcggcggg gcgctttacg
     1201 ccggcggcga gcccggccgc gttgccgcga actacaccta tcacaacatg gccggccaag
     1261 gcatccacgc ctatggcgcc ggcgcggagt ggttcgtggc cccaaacgcg accttagcgg
     1321 ttcgcggcgg cggcctgtca cgggatcaca ttgacggcgg ctatgtcggc gcgcagggga
     1381 cttggtatct catccccgat ttcgcgttga gcgccggtgt cgatcatttg tcggttggat
     1441 atcatgagac gggcgagacc atccaagccg aatggttgcc gtcgcaaacc actccgatat
     1501 cgatctacgc cggttatcag catctcgact attccgtagc cagcgcggat ttgatttttg
     1561 ccggcgtcaa attgtacctg aacggcgacg gcgcggccac gctggccgat cgccaacgcc
     1621 agggcacgaa cggctatatc gtccaatcgc ccaaggatct tgaccagtac taagtctttt
     1681 ctgaaccagc tgaagcgggc tctccttcgg gagggctcgt ttgcatttgg gcgctagagt
     1741 gtgatccgat ttaattgaat cggatcacac tctcgatttt ttgtttttcg cgcaatctga
     1801 cggcaaaccg cgtcggcacg ctgctgctac cgaccacttt gccggattgc gctctagccc
     1861 gcgcgccggg ctcccagccg caccccgggc gaggggcgtt cgcgcacgac ctcgcggcgg
     1921 aagcggaaca gctccgccgg ccggccgcgc gtctgggtgg agaactcgcc ggtgccttcc
     1981 accaaaccct gggcttccac cagccggcgg aaattctgct tgtgcaggcg gatgcccgaa
     2041 atcgcttcca ccgtgcgctg caactccagc agcgtgaagg acggcggcat cagctcgaac
     2101 accaccgggc ggtacttcat cttcccgcgc aggcgcgcga tcgccgtggc gaggatgcgg
     2161 cgatggtcga agatcatgcc ggtccctaag agcaagagcc cggcggcgct cgcccgtccg
     2221 tcgcgcacgc tttcctgaac cagccccgct tcgtagagca gttcgtagcg ctccagaact
     2281 ttttcttcgt cccaggccag acggtccagt ccgaagcaaa gccgggcgcg gtcgcgccgc
     2341 tgttcggcgg ttttcgaatc cggcgcggat ttggcgaatg aagccaacgc tggcgcgatc
     2401 gcttcgtcga tcaaggcggg ccgttcggcg cgccagtcct cccacggaaa atagcggtac
     2461 cagtcgcgcc acagcgtggc cggcgccttg agttttccgc attgacgggt cagcgccaga
     2521 tagccgacgg aaacgacgcg cgggccctcg ccgggtttga gttcgtggcg gccgcgatcg
     2581 ccgaaagtgt aaagctgctc ggcataaccg aggtcgagat aggtctgctc gcccacccag
     2641 gtccgcaggc cgctttccaa ggtgcggtgc aggagcggat cgaacgggcc gaagggcagg
     2701 gcgtcgccgc cgcttgcggg attgcgcacg atcaggacgg aagggcgttc ctcgacgacg
     2761 gaaacgatgg ccgccgagag gccgatggcg acaacatgtt tggccgggct catcggcctg
     2821 cgatggtcag gggcaattcc accggaatgc cctcgtcgac cagcctgcca ggacccatgg
     2881 cttccaccgc ccgcaccatc cgcccattcc ggtccagcga ctggtccgcc agccgcacca
     2941 gcagccggtt gggctgcgca tgcgaagccc tggcgcgaat ttcctgcccg atctcgtatt
     3001 cgctgccggg tccggcacgg tcgcataata tggtgaaggc ggcggccatg gaacggctga
     3061 cgccggccca gcaatgtacc agcatgggct ccgcggcgtc ccatttgcgg ctgaacgcaa
     3121 tcagttgcgc aacatgccgc tcgccgggag gcgccaggcc cgaacttgga tcggcgatgt
     3181 cgttcaagct gaggcggaga tggcgttctg gagcgatgcc ctccggcgta tcgatcatgt
     3241 cctcgggcga caatagggtc accagatgcg aaggatgata cgcacggatg gaatcgagga
     3301 cggcggaaag cggcgtgacg agaatcatgg tcatgggacc attctgctag cggaacatta
     3361 tggcacgcaa aagtcgcgat tcggggcggg ttgacgcggg gctagctgga cagacggcga
     3421 aaatgctcta ggaacagagc ttgggcctcg gcggttgcga gcggcgccaa gcggggtgtt
     3481 ctaagggcct tgggcggggt cccgaaatag cgtttggcct ctcccggctc aaatccagcg
     3541 agct
//
LOCUS       DASWPU010000655         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_30086,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000655 DASWPU010000000
VERSION     DASWPU010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30086"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VGC27_14000"
     CDS             <1..673
                     /locus_tag="VGC27_14000"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEY0283722.1"
                     /translation="GKTAINTAEGKNLVGVFHQPKIVLADTGVLSTLPRRELLAGYAE
                     MVKYGALGDAAFFEWLEANAGKALDGDDAAIVHAVAHSCRMKAGIVARDEHEAGERAL
                     LNLGHTFGHALEAATGYSERLLHGEGVALGSALAFRLSVKLGLCSGQDAERFIRHLKT
                     LGLPADIADIPGPQPSADELLGHMTHDKKVKDGKPAFVLLHGIGRAFVTSDVPREAVR
                     DVLAG"
     gene            736..>1584
                     /locus_tag="VGC27_14005"
     CDS             736..>1584
                     /locus_tag="VGC27_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEY0283723.1"
                     /translation="MYTSLLIPFAAIAVLMIAAAFFAGSETALTAVSHGKMHQLEKDG
                     SRAAGAVNKLIHNRERLIGALLLGNTFMEVLASAIATSVFEVRFGPQAVAIVAVIMTL
                     LILVFSEVLPKTLAIARTERFALAVATPVRHAVTILGPIARSVQLFVWKVLRLFGVKE
                     EDVGMAALAHDEIRGTVFLRHKEGMVERESRDMISGVLDLRELTASDVMIHRKNMMTV
                     SIDQNAEQIVRALMATHHARVPVWREQPENIVGVLSTKDMVRAVLAHRGVLQDLDIAA
                     LVTPPWF"
BASE COUNT          271 a          498 c          533 g          282 t
ORIGIN      
        1 cggcaagacg gcgatcaata cggcggaggg caagaacctg gtcggcgtgt tccatcagcc
       61 caagatcgtg ctggccgaca ccggcgtgct ctccaccctg ccccggcgcg aattgctggc
      121 cggctatgcg gaaatggtga agtacggcgc gctgggcgac gcggcattct tcgaatggct
      181 ggaagcgaac gccggcaagg cgctcgacgg cgacgacgcg gcgatcgtcc atgccgtggc
      241 gcattcctgc cgcatgaagg ccgggatcgt ggcgcgcgac gaacatgagg cgggcgaacg
      301 cgccttgctc aatctcggcc acactttcgg gcatgcgctg gaagcggcga cgggttattc
      361 ggagcggctg ctgcacggcg aaggcgtggc gctggggtcg gcgctggcgt tccggctctc
      421 ggtgaagctc ggcctgtgca gcggtcagga cgcggagcgc ttcatccggc atctgaagac
      481 tctcggcctg cccgctgaca ttgctgacat tcccgggccg cagccttcag ccgacgaact
      541 tctgggccat atgacccacg acaagaaagt caaggacggc aagccggcct tcgtgctgct
      601 gcacggcatc ggccgagcct tcgtcaccag cgacgtgccg cgcgaggcgg tgcgggacgt
      661 gctggcaggg tagctttccg ccttcccgca gaacgggagg taaacgatac tcccttcccc
      721 gcttcgaggt ttgccatgta cacgtcactt ctgatcccct tcgcggcgat tgccgtgctg
      781 atgattgccg ccgcgttttt tgccggttcc gagaccgcgt tgaccgcggt ttcgcacggc
      841 aagatgcatc agctcgaaaa ggacggctcg cgcgcggcgg gggccgtcaa caagctgatc
      901 cacaaccgcg agcggctgat cggcgccttg ttgctgggca ataccttcat ggaagtcctg
      961 gcctcggcga tcgcaacctc ggttttcgag gtcaggttcg ggccgcaggc ggtggcgatc
     1021 gtggccgtca tcatgaccct gctcatcctc gtgttctccg aagtgctgcc caagacgctg
     1081 gcgatcgccc gcaccgaaag gtttgcgctg gcggtcgcaa ccccggtacg gcacgccgtg
     1141 acgatcctcg gccccatcgc ccggtcggtg cagttgttcg tctggaaggt gctgaggctg
     1201 ttcggagtca aggaagaaga tgtggggatg gcggcattgg cgcacgacga aatccgcggc
     1261 acggtcttcc tgcgccacaa ggaaggcatg gtcgagcgcg aaagccgcga catgatcagc
     1321 ggcgtgctcg atctgcgcga gctcaccgcc agcgacgtta tgatccatcg caagaacatg
     1381 atgacggtct ccatcgacca gaacgccgag cagatcgtgc gggcgctgat ggcgacgcat
     1441 cacgcgcgtg tgccggtgtg gcgcgaacag ccggaaaaca tcgtgggcgt cctgagcacc
     1501 aaggacatgg tgcgcgccgt gctcgcccat cgcggcgtgt tgcaggatct cgatatcgcc
     1561 gcgctggtca ccccgccctg gttc
//
LOCUS       DASWPU010000656         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_54785,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000656 DASWPU010000000
VERSION     DASWPU010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54785"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            520..714
                     /locus_tag="VGC27_14010"
     CDS             520..714
                     /locus_tag="VGC27_14010"
                     /inference="COORDINATES: protein motif:HMM:NF041949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thivi_2564 family membrane protein"
                     /protein_id="HEY0283724.1"
                     /translation="MSLVSLVIVLIVVGVLLWLVNTYIPMDGKIKSILNAVVVIAVVL
                     WLLSVFGVLDNLSTFRVGRA"
     gene            complement(753..2183)
                     /locus_tag="VGC27_14015"
     CDS             complement(753..2183)
                     /locus_tag="VGC27_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0283725.1"
                     /translation="MSFSEYGRCDGLGLAEHVARKDVTAAELLEEAVARAEKLNPALG
                     AIVFKDYEAARAAAQAPLPSGPFAGVPFLFKDIFLNAKGTPTRQGSRFFPPFPADHDS
                     FLAVRFKKAGLIAFGKTNVPEFGLLPTTEGRLYGPAHNPWNLAYSTGGSSGGSAAAVA
                     AGIVPLAHANDGGGSIRIPASCCGLVGLKPSRGRVSAGPDMGEAVDGLSCELVLSRSV
                     RDTAAALDVAAGYEPGDPYCAAPAPASYLKAAQEKPKRLRIGFANKKLGGSPLHPDCL
                     AAVEGAAKLCADLGHSVEEASPDLDVAMLLPSFMAIWTSNLASFVDLIARLTGQTPSM
                     DNLEGTTIGLYEAGKRVGASEYLQAKMMLNQVARTAAKFHRSHDLWLTATLGAPPLRL
                     GTLDIDERDPQKAFAPLIDYVAYTALQNVTGQPAISLPLHWNDAGLPIGVHFAAPYGD
                     ELTLLRLAAQLEHAAPWSGRYEAIKL"
BASE COUNT          395 a          709 c          697 g          403 t
ORIGIN      
        1 gccggcaagt tcaagcccgg caaaatggaa aagctccaca ggaaaatccc tggccgcctc
       61 gcggttctca agcccgggca gacggggtga gtagattccg aaccttcccc cccgcaatcg
      121 gcaatggcat cacttcaaag acctcaaacg gctccctgac tggcaaatca ctttcggagc
      181 cctcaatcgt ggctttcccc ttcaccatat tgagggctcc gtttttttcc gggagactgt
      241 tccataggcc acatcattcg attccagcgc ccggaaacgc cttacggcgt atccaaggtg
      301 catgttcccg aggaagcaaa cgcggcccag cagcttcgcc gtttggaagc gctcggctat
      361 accatccttg aagtcacgcc gccgcttgcg gcagccggaa agtccagtgg ccgcaatacc
      421 cgagtttaag cgccgcccgg cggcgagggc tgagtagatt ccgaacctac cgtcgaagga
      481 tggcggccgg ataccttcgc catatcgaag gagattctca tgtcgctcgt tagcttggtc
      541 attgtcctga tcgtcgtcgg cgttctgctc tggctcgtca atacctacat cccgatggac
      601 ggtaagatta agagcatcct caacgccgtc gtcgtgatcg cggtggttct atggctcttg
      661 agtgtgttcg gcgtcctcga caacctctcg acattccgtg tcggcagggc gtgatacggc
      721 cgttagatcg agagtaacgc cggcattcac cgtcacagct ttatcgcttc atacctcccg
      781 gaccacggcg ccgcgtgttc gagttgagcg gcgaggcgaa gcagcgtgag ctcgtcgccg
      841 tagggcgccg cgaaatgcac gccgatgggc aggccggcat cgttccaatg caagggcagg
      901 ctgatcgctg gctggccggt gacgttctgg agcgcggtgt acgccacata gtcgatcagc
      961 ggcgcgaagg ctttttgcgg atcgcgttcg tcgatgtcga gggtgccgag cctcagcggc
     1021 ggggcgccca gcgtggccgt cagccacaaa tcatggctgc ggtggaattt tgccgccgtg
     1081 cgcgccacct gattgagcat catcttggcc tgaaggtatt cggaagcgcc gacgcgcttg
     1141 cccgcttcgt aaaggccgat ggtggtgcct tccagattgt ccatggacgg agtttgcccc
     1201 gtcagccgcg cgatcagatc gacgaacgag gcgaggttgc tggtccagat cgccatgaag
     1261 gacggcagca acatcgccac atcaagatcg ggcgaggctt cttccacgct atggccgaga
     1321 tccgcgcaga gcttggcggc gccttccacc gcggcgaggc aatcggggtg cagcgggctg
     1381 ccgcccagct tcttgttcgc aaagccgatg cgcaggcgct tcggcttttc ctgcgccgcc
     1441 ttgagatagg aggcgggcgc gggcgcggcg caatagggat cgcccggctc gtagcccgcc
     1501 gccacgtcga gcgccgccgc cgtgtcgcgc acgctgcgcg agagcaccag ctcgcaggac
     1561 aagccgtcca ccgcttcgcc catgtcaggc ccggccgaaa cgcggccgcg cgagggcttc
     1621 aagccgacca ggccgcagca cgaggcggga atgcggatcg aaccgccgcc gtcattggca
     1681 tgggccagcg gcacgatgcc ggccgccacc gccgccgccg accctcccga cgagccgccg
     1741 gtggaatagg ccaggttcca cggattgtgc gccgggccgt agagcctgcc ttcggtcgtc
     1801 ggcagcaggc cgaattccgg cacgttggtc ttgccgaagg cgatcaggcc cgccttcttg
     1861 aaccgcaccg ccagaaaaga gtcgtgatcg gcgggaaaag gcgggaagaa gcgcgagcct
     1921 tgccgcgtgg gcgtgccctt cgcgttcaga aaaatgtcct tgaacaggaa tgggacgccg
     1981 gcgaacggcc ccgatggcag cggcgcttgc gcggcggccc gcgccgcctc gtagtccttg
     2041 aagacgatgg cgcccagcgc ggggttcaac ttctcggcgc gcgcgaccgc ttcttccaga
     2101 agctccgcgg cggtgacgtc tttcctggcc acatgttcgg cgagacctaa tccgtcgcag
     2161 cggccatatt cgctgaagga catgtttcct ccgcccgttt acca
//
LOCUS       DASWPU010000657         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_103479,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000657 DASWPU010000000
VERSION     DASWPU010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103479"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1631
                     /gene="sdhA"
                     /locus_tag="VGC27_14020"
     CDS             <1..1631
                     /gene="sdhA"
                     /locus_tag="VGC27_14020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY0283726.1"
                     /translation="GVAAALGNMGEDDWRWHMYDTVKGSDWLGDQDAIEYLCKNAPEA
                     VYELEHFGVPFSRTEDGKIYQRAFGGMTSHFGQGVAQRTAAAADRTGHAMLHTLYGQC
                     VKHEVNFFVEYFALDLIMEDGAVRGLMAWCLDDGTIHRFRARKVILATGGYGRIYFTC
                     TGAHTQTGDGNGMVLRAGLPLQDMEFIQFHPTGVRGVGVLITEGVRGEGGFLTNSEGE
                     RFMERYAPHAKDLASRDVVSRAITVEIREGRGCGPDKDYANLHLAHLDPKIIHERLPG
                     IAESARIFAGVDVTKDPIPVQPTVHYNMGGIPTNHLGEALTLRNGEPDAVVPGLMAVG
                     EGACVSVHGANRLGSNSLIDLVVFGKAAGEQAARTLEGRSNNHADLPKHAGEEALARL
                     DRLRHAKGKTSTAEMRLAMQKAMQEDAAVFRTGETLAKGKQRIADIYAAIGDIGVSDR
                     SLVWNTDLVETLEYENLIAQASVTIAGALNRTESRGAHAREDYPERDDANWMKHTLSW
                     FDAKTGEVRLDYRPVHTYTLTYEVEYIPPKARVY"
     gene            complement(1776..>2187)
                     /locus_tag="VGC27_14025"
     CDS             complement(1776..>2187)
                     /locus_tag="VGC27_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283727.1"
                     /translation="IYGMKLPSEIYAKHNFHTRMKSAEIPPPKIHLVAKNRLTQYVRT
                     AKGYAAVLDGIQNDLTAVHKTNPEYFTFKNIDDGMFEIRDFQTAGVVAFARGTPFSTM
                     NSGTLSVATQRVQVNKEQLDQNRAIINTLAKKLW"
BASE COUNT          430 a          700 c          662 g          395 t
ORIGIN      
        1 gcggcgttgc cgcggcactc ggcaacatgg gcgaggacga ttggcgctgg cacatgtacg
       61 acacggtgaa gggatcggac tggctcggcg atcaggacgc catcgagtat ctctgcaaga
      121 acgcgccgga ggccgtctac gagctggagc atttcggcgt gccgttctcg cgcaccgagg
      181 acgggaaaat ctatcagcgc gccttcggcg gcatgaccag ccattttggc caaggcgtcg
      241 cccagcgcac cgccgccgcc gcagaccgca ccggccacgc catgctgcac acgctgtacg
      301 gccagtgcgt caagcacgag gtgaatttct tcgtcgaata ttttgccctc gacctgatca
      361 tggaagacgg cgccgtgcgc ggcctgatgg cctggtgcct ggacgacggc accatccacc
      421 gcttccgcgc ccgcaaggtg atcctggcga ccggcggcta tggccgcatc tatttcacct
      481 gcaccggcgc gcacacccag accggcgacg gcaacggcat ggtgctgcgc gccggcctgc
      541 cgctgcagga catggagttc atccagttcc atcccaccgg cgtgcgcggc gtcggcgtcc
      601 tgatcaccga gggcgtgcgc ggcgaaggcg gcttcctcac caattccgaa ggcgagcgct
      661 tcatggagcg ctatgcgccc catgccaagg acctggcctc gcgcgacgtg gtcagccgcg
      721 ccatcaccgt agaaatccgc gaaggccgcg gctgcggccc cgacaaggac tacgccaacc
      781 tgcatctggc gcatctcgac ccgaagatca tccacgagcg cctgcccggc atcgccgaga
      841 gcgcgcgaat cttcgcgggc gtcgatgtca ccaaggaccc catcccggtg cagccgacgg
      901 tccactacaa tatgggcggc attcccacca accatttggg cgaagcgctc accttgcgga
      961 acggcgaacc cgacgcggtg gtccccggcc tgatggcggt gggcgaaggc gcctgcgtct
     1021 cggtgcatgg cgccaaccgg ctgggctcga actcgctgat cgatctggtg gtgttcggca
     1081 aggcggcggg cgaacaggcg gccaggacgc tggaaggccg cagcaacaac cacgccgacc
     1141 tgcccaagca tgcgggcgaa gaagccctgg cgcgcctgga cagactccgc cacgccaagg
     1201 gcaagacctc caccgccgag atgcgcctcg ccatgcagaa ggcgatgcag gaagacgccg
     1261 ccgtgttccg taccggcgaa accctcgcca aaggcaagca gcgcatcgcg gacatctacg
     1321 ccgccatcgg cgatatcggg gtcagcgacc gctcgctggt gtggaacacc gatctggtgg
     1381 aaacgctgga atacgagaat ctaatcgccc aggcctcggt caccatcgcc ggcgcgctca
     1441 accgcaccga aagccgcgga gcgcacgcgc gcgaggatta tcccgagcgc gacgacgcca
     1501 actggatgaa gcacaccttg tcgtggttcg acgccaagac cggcgaagtc cgcctcgact
     1561 accggccggt ccacacctac acgctgacct atgaggtgga gtacatcccg ccgaaagcaa
     1621 gggtgtattg atctttcatc cctctcccct ttgtgggaga gggcgggttg ccttgcactt
     1681 acgaagcgcg agcgcagcga gcgccgtaag tgcttaggca aaccgggtga ggggtaacgt
     1741 gctcctgaca ccgccgcccc tcgaattcgc ccttgctacc agagcttctt agcgagcgtg
     1801 ttgattatag ctcggttctg gtcgagttgt tctttattca cctgaactct ttgtgtcgca
     1861 acactcagag ttccagaatt catcgtagag aatggcgttc cacgcgcgaa tgcaactaca
     1921 ccagcggttt gaaagtctcg gatttcaaac ataccatcgt caatgttctt gaaggtgaag
     1981 tattcaggat tcgtcttgtg gaccgccgtc aggtcgttct ggataccgtc gagaacagct
     2041 gcatatccct tagcagtcct aacatattgg gttagtctgt tcttcgccac cagatgaatt
     2101 ttcggcggag gtatttcggc gcttttcatc cgtgtatgga agttgtgttt cgcgtaaatc
     2161 tcagatggaa gcttcattcc atatatg
//
LOCUS       DASWPU010000658         3413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_79354,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000658 DASWPU010000000
VERSION     DASWPU010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79354"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="VGC27_14030"
     CDS             complement(<1..1367)
                     /locus_tag="VGC27_14030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0283728.1"
                     /translation="MLSPTQQKKAAEGVAPLRAAQDAERLQLAVTGASVVAFSWTIGD
                     DVIVWDGARDILPPAVVAGITNRGKTLLGLMSPEGRNKLDALIENRVRENVHFDIDVE
                     LASALGSVWYVMLGVRISDADGATERLTGIMREITEQKRESQRLNYLATRDELTGHLN
                     RNSLRAELAEAIERANREERHCAFLVASIDRLAMINDSYGFGAADEVIVAVGERLSRS
                     LRSSDVIGRTAGNKFGVILRNCTDQEIVIVADRLRAAVRGSVIETRSGQVAATTSVGA
                     VWLPSGASTSQEAMLRAEEALERARIGGRDGFTMYRDSPQREGARLRLMAMADEVAAA
                     LKDKRLVFAYQPIVNARTRKAVEYECLLRMVREDGSITAASQFIPAAEKLGLVRLVDR
                     HALEMVVATLHAYEGVTLSVNVSGTTSRDTAWLQSFVDYVQENASIARRLVVELTETA
                     ALNHF"
     gene            complement(1784..2371)
                     /locus_tag="VGC27_14035"
     CDS             complement(1784..2371)
                     /locus_tag="VGC27_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283729.1"
                     /translation="MISAFAAVLALSAAAAWMTAKGARPASRLYLRFACVLFVALAAS
                     AFAGVAPEAVALIATTLATALLMLAAAAGFRSAVKPPAAASVLALACAGGIWSAASGA
                     MAPAVVLQVFCLLVMIALAVRERRSGLRLGLASAALLAAACSLLVENSLAFVALLLFS
                     AAGLLGTTLALARASEIPLEGRRRSRGAAAIRGMD"
     gene            complement(2406..3296)
                     /locus_tag="VGC27_14040"
     CDS             complement(2406..3296)
                     /locus_tag="VGC27_14040"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEY0283730.1"
                     /translation="MKRVFLAVLFLSAALAGATANAFAGEGRPVLVELYTSQGCGACV
                     PADKLLIKLAKRKGLIAVSLPITYWDMLGWKDTLASEANTRRQKAYAGAMGRGGVYTP
                     QIVIDGVKDVVGSHEDLVEGAVEAAEEARDDASEAIADARDALDDASDGDLPIGVSAF
                     GALRRKTEMLTARLPPSVDIGLKRMPQKLNVAIAAAPDVAAKNKLNATIWLLRLRSAV
                     TVRIDAGENAGRTVTYRNVVSDIKELGRWNGRAVSIDLPRVDAKNPPHDGVVVVLQQG
                     GYGRVLGAAYLGHAEFYAQQ"
BASE COUNT          584 a         1160 c         1114 g          555 t
ORIGIN      
        1 tcgaaatggt tgagggccgc ggtttcggtc agctcgacca ccaggcggcg ggcgatggag
       61 gcgttctcct ggacataatc gacgaaggat tgcagccagg cggtgtcgcg cgaggtggtg
      121 cccgagacgt tgacgctcaa ggtgacgccc tcgtaggcgt gcagcgtggc gacgaccatt
      181 tccagcgcgt gacggtcgac caggcggacc aggccgagct tttcggccgc gggaatgaac
      241 tggctggcgg cggtgatgct gccgtcttcg cgcaccatcc gcagcaggca ttcgtattcc
      301 acggctttgc gcgtgcgggc gttgacgatg ggctggtagg caaacaccag acgcttgtcc
      361 ttcagcgcgg ccgcgacttc gtccgccatc gccataaggc gcaggcgcgc gccttcgcgc
      421 tggggcgaat cccggtacat ggtgaaaccg tcgcggccgc ctatgcgggc gcgctcaagc
      481 gcttcctccg cgcgcagcat cgcttcctgg ctcgtcgaag cgcccgaagg cagccacacc
      541 gcgcccaccg aagtggtcgc ggccacctgt ccgctgcggg tttcgatcac actgccgcgc
      601 accgccgctc gcagcctgtc ggcgacgatg acgatctcct gatccgtgca gttcctcagg
      661 atgacgccga acttgttgcc cgcggtgcgg ccgatcacgt cgctggagcg cagcgagcgc
      721 gacagccgct cgccgaccgc gacgatcact tcgtcggcgg cgccgaaacc gtaggaatcg
      781 ttgatcatcg ccagccggtc gatggaagcg accaggaaag cgcaatggcg ctcctcgcga
      841 ttggcccgtt cgatggcctc ggccagttcg gcgcgcaacg aattgcgatt gaggtggccg
      901 gtcagttcgt cgcgcgtggc gaggtaattc agcctctggc tttcgcgctt ttgctcggtg
      961 atctcgcgca tgatgccggt gaggcgttcg gtggcgccgt cggcgtcgga aatgcgcacg
     1021 cccagcatga cgtaccacac cgaaccgagc gcgctggcca gttcgacgtc gatgtcgaaa
     1081 tgcacgtttt cgcgcacgcg gttctcgatc agggcgtcaa gcttgttgcg tccttccgga
     1141 ctcatcaggc cgagcagcgt cttgccgcgg ttggtgattc cggcgacgac ggcaggcggg
     1201 agaatatcgc gcgcgccgtc ccagacgatg acgtcgtcgc cgatggtcca actgaacgca
     1261 acgacgctcg cgcccgtcac ggccagttgc agccgttcgg cgtcttgcgc ggcgcgcaaa
     1321 ggagcaacgc cttccgcggc cttcttctgt tgtgtcgggc tcagcacagt ccaccacccc
     1381 aagccgattt tcggcctttg cgggtgatga tgacgttgcc gccttaaaaa actcgtaacc
     1441 tctgaggcaa tcggagcagt ttcggcgcgc ggtccgcact ttgccttaag aacgggttat
     1501 gacggcgatc gcggcttttc cacgtcttgc cagtccggcg gcactccccc gcaacggaca
     1561 gagttgcgtc gtcgagaaag ccagagcccg caggcaattc gatggtgaat acacgatgcg
     1621 gcagcgggcg caaacgcgcg tcgcgcccca tcgcgcggca agcaatccgt gccaatccgg
     1681 cacagaagac catgcttctt tctggaacgg ggagcgcctg atgccgtcgt tcacggccca
     1741 ggtcatcggc caagccctgg gcaggccgcg cgcgcaggcc gcttcagtcc atgccgcgta
     1801 tggcggccgc gccgcgggag cggcgccgtc cttcgagcgg tatctctgac gcccgcgcca
     1861 gcgccagcgt ggttcccagc aaaccggcgg ccgaaaacaa cagcagcgcc acgaacgcca
     1921 gcgaattttc caccagcagc gaacaggcag cagccaggag agcggcagag gccaagccaa
     1981 gacgcagacc ggaacggcgc tcgcgcacgg cgagcgcgat cataaccaac agacaaaaga
     2041 cctgaagaac gacggccggc gccatcgcgc cgctcgcggc ggaccagatg ccgccggcgc
     2101 aggctagcgc cagtaccgaa gcggccgccg gcggtttgac cgcactgcgg aatcccgcag
     2161 cggccgccag catcaagagc gccgtcgcca gtgtcgttgc gatcagcgcg accgcttccg
     2221 gcgcgacgcc ggcgaacgcg ctcgccgcca gcgcgacaaa aagcacgcag gcgaaacgca
     2281 gatagagtcg cgacgcgggc cgcgcgcctt tcgccgtcat ccaggcggcg gcggcggaga
     2341 gcgcaagaac ggcggcaaaa gcgcttatca ccgcttcgcc ttgcgccgga aattcgtcat
     2401 ggccgctatt gctgcgcgta aaattcggcg tggccgagat aagccgctcc gagcacgcgg
     2461 ccgtagccgc cttgctgcag gaccacgaca acgccgtcat gcggcgggtt cttggcgtcg
     2521 acgcgcggca gatcgatgga tacggcgcgg ccgttccagc ggcccagctc tttgatgtcg
     2581 ctcacgacgt tgcgataggt caccgtgcgg cccgcatttt cgccggcgtc gatgcgcacg
     2641 gtgacggcgg aacgcaggcg cagcagccag atcgtggcgt tgagcttgtt cttcgccgcg
     2701 acgtccggcg ccgcggcgat ggcgacattc aatttctgcg gcatccgctt gaggccgata
     2761 tcgacggacg gcggcagccg cgccgtcagc atctccgtct tgcggcgcag cgccccgaac
     2821 gccgacacgc caatcggcag atcgccgtcg gaggcgtcgt ccagcgcatc ccgcgcgtcc
     2881 gcgatcgcct ccgaggcgtc gtcacgcgct tcctcggccg cttcgaccgc cccctcgacg
     2941 aggtcttcgt ggctgccgac cacatccttc acgccgtcga tgacgatctg gggcgtgtaa
     3001 acgccgcccc gtcccatcgc ccccgcatag gctttctgcc gacgggtgtt cgcttcgctg
     3061 gccagcgtgt ccttccagcc cagcatgtcc caataggtga tcggcaggct gaccgcgatc
     3121 agtcccttgc gcttggccag cttgatcagc agcttatcgg ccggcacgca ggcgccgcag
     3181 ccctggctgg tgtaaagctc gaccagaacg ggccggccct cgcccgcaaa ggcgtttgcc
     3241 gtcgcgcccg ccagcgccgc cgacagaaaa agaaccgcca ggaaaacccg tttcatgtct
     3301 gaaccatagg gcgggggcgg gcggctcacc agtcacttcg gcttgaggtg tgttgcggaa
     3361 ttcgggctgc aagcagccgg gaaggcgctc aaccgccctc gtcaccgcgc cga
//
LOCUS       DASWPU010000659         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_5994, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000659 DASWPU010000000
VERSION     DASWPU010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5994"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /gene="uppS"
                     /locus_tag="VGC27_14045"
     CDS             complement(<1..542)
                     /gene="uppS"
                     /locus_tag="VGC27_14045"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY0283731.1"
                     /translation="MSTDIDARINALQKAEGLPRHIAVIMDGNGRWAKQRHRPRIVGH
                     HAGAEPVRKCVRTCSKLGLTHLTLYTFSIENWTRPKTEVIGLMAFLEEVLRREVLELD
                     QNNVRLNAIGRLDALPAATRRTVERSIETLSKNTGLVLTLCLSYGGRAEIVDAARSLS
                     QRVASGELAAGDIDEDMFGKA"
     gene            complement(558..1118)
                     /gene="frr"
                     /locus_tag="VGC27_14050"
     CDS             complement(558..1118)
                     /gene="frr"
                     /locus_tag="VGC27_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY0283732.1"
                     /translation="MVLDELYHKTEDHMKKTLEMLTSELGGIRTGKASPALLDSLRVN
                     YYGSMVPVKQVANVSVPDPRMITIQPWDRGIIQEIEKAIRASELGLNPQNDGGLIRLP
                     IPPLTEERRKDLVKVVKRIGEDSKVAIRNVRRDANDRIKKLEKEHDLSEDDMRAKQDA
                     IQKLTDSFVHKVDEAVTAKEKDILEI"
     gene            complement(1134..>1820)
                     /gene="pyrH"
                     /locus_tag="VGC27_14055"
     CDS             complement(1134..>1820)
                     /gene="pyrH"
                     /locus_tag="VGC27_14055"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY0283733.1"
                     /translation="LLKFSGEILARDDGGGIDFAKLSSFCKQIAAVKAEGIEIGLVIG
                     GGNLIRGASISENGIDRVTADYMGMLATVINALAFQSALERAGVDTRVQTAIKMEQLA
                     EPYIKRRAIRHLEKGRVVIFAGGTGNPYFTTDTAAALRAIEIEADVVMKGTKVDGVYD
                     GDPMRDPNAKRFDSLTYIDVLNRRLGVMDATAISLCMENKLPVIVFKLTGENNLIRAV
                     RGEAVGTVVK"
BASE COUNT          297 a          553 c          587 g          383 t
ORIGIN      
        1 gctttgccaa acatgtcttc gtcgatatcg cccgcggcca gctcgccact ggcgacacgc
       61 tgcgagaggc tccgggccgc gtccacgatt tcagcacggc cgccgtagga gagacacagg
      121 gtcaacacga ggcctgtatt cttcgagagc gtctcgatgg aacgctccac cgtgcggcgc
      181 gtggcagccg gcaacgcgtc cagccgcccg atcgcgttca ggcgcacgtt gttctgatcg
      241 agctccaata cttcgcggcg cagcacttct tccaggaatg ccatgagccc gatgacctcg
      301 gtcttggggc gcgtccagtt ttcgatcgag aacgtgtaga gggtgaggtg ggtcagcccg
      361 agcttggaac acgtgcgcac gcatttgcga accggctccg cgcccgcgtg gtgccccacg
      421 atgcgcgggc ggtggcgttg cttggcccag cggccgttgc cgtccatgat gacggcgatg
      481 tggcgcggca gtccttccgc cttctggaga gcgttgatgc gcgcatcaat gtctgtactc
      541 ataggatgtg cgcgcgccta gatttccagg atgtccttct ccttggcagt gacggcctcg
      601 tcgaccttgt gcacgaagct atccgtgagt ttctggatgg cgtcctgctt ggcgcgcatg
      661 tcgtcctcgg agagatcgtg ctctttttcg agcttcttga tgcggtcgtt ggcgtcgcgg
      721 cgcacattgc ggatggccac cttggaatcc tcgccgatgc gcttgaccac cttgacgagg
      781 tccttgcggc gctcctcggt gagcggcgga atgggcagac gaatgaggcc gccgtcgttc
      841 tgcggattca agccgagctc cgacgcgcgg atggccttct cgatttcctg gatgatgccg
      901 cggtcccagg gctggatggt gatcatgcgc ggatcgggca cggagacgtt ggccacctgt
      961 ttcacgggca ccatcgatcc gtaataattc acgcgcagcg aatcgagcag ggccgggctg
     1021 gccttgccgg tccggatgcc gcccagctca ctggtcagca tctccagcgt ctttttcatg
     1081 tggtcttcgg tcttgtgata cagctcgtcg agcaccatgg ggaaaaacct ccgctacttg
     1141 acgacggtgc cgacggcttc tccgcgcacc gcacgaatca gattgttctc gcccgtcagc
     1201 ttgaacacga tgacggggag tttgttctcc atgcacagcg agatggcggt tgcgtccatc
     1261 acgccgaggc gacggttgag aacgtcgatg taggtgagtg aatcgaagcg cttggcgttg
     1321 ggatcgcgca tgggatcgcc gtcgtacacg ccgtccacct tggtcccctt catcaccaca
     1381 tcggcttcga tctcgatggc gcgcagcgcg gccgcggtgt cggtggtgaa gtagggattg
     1441 ccggtgccgc cggcgaaaat gacgacgcgc cccttctcca ggtggcggat ggcgcgacgc
     1501 ttgatgtacg gctctgccag ctgctccatc ttgatggcgg tctgcacacg ggtgtcgacg
     1561 ccggcgcgct cgagcgcact ctggaacgcg agtgcgttga tcaccgtcgc cagcatgccc
     1621 atgtagtcag cggtgacgcg gtcgatgccg ttttcggaga tggaagcgcc gcggatcaga
     1681 ttgccgccgc cgatgacgag accgatctcg atcccttccg ccttgacggc tgcaatctgt
     1741 ttgcagaaag aagaaagctt ggcgaaatcg atgccgcccc cgtcgtcgcg ggcgaggatt
     1801 tcaccgctga acttgagcag
//
LOCUS       DASWPU010000660         1779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_6058, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000660 DASWPU010000000
VERSION     DASWPU010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6058"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VGC27_14060"
     CDS             <1..210
                     /locus_tag="VGC27_14060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0283734.1"
                     /translation="KVVDSFSLGKDYAKTLREWSSRMREAKNEIFALGHDERFLRNWQ
                     FYLGICAASFSVERTDVVQVELVNA"
     gene            531..>1779
                     /locus_tag="VGC27_14065"
     CDS             531..>1779
                     /locus_tag="VGC27_14065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0283735.1"
                     /translation="MTNKIALSAFKLVVFGTALFCVFAFWRTAISMGLRLPLGPNEGW
                     NAYHAAAAMSGGPLYPGPHSMMINNYPPLSFYLIGAFGRVLDDNILAGRIVSLLSFFL
                     IILGIFAAALRLGCKRLEASFAALLFAGGMLVFTDYVGMNDPQMLAHAIAMAGFLLLL
                     KAPRGTVHVALAALLFVLAVFVKHNVIAMALAATVWLIDYDRKNALRLAGLGVAFLLA
                     GLVLFRLIYGTGLLAELISARIYSIDNLHAGFDTWLRWSLVPLVGLTVLASLRWPDRY
                     VRLVSFYAAAGTILGLCFLGGAGVDANAMFDADIALALGAALLVQQAARGAFAPVTAA
                     AYVLPLLLGAWTNAGWRDAHGWLHPMREETAAAQKDIAFLAARPGPALCEMQSFCYWA
                     RKPAAVDVFNLSQQFATGARSDAK"
BASE COUNT          257 a          583 c          554 g          385 t
ORIGIN      
        1 aaggtggtgg acagtttttc cttaggcaag gattacgcca agaccctgcg cgaatggtcc
       61 tcgcgcatgc gcgaggcgaa aaacgaaatc ttcgcgctcg gccacgacga acgtttcctg
      121 cgcaactggc agttttatct cggcatctgc gcggcgtcgt tcagcgtcga acgcaccgac
      181 gtggtgcagg tcgaactggt caacgcataa atccgcccgc tcgtctccgt cgcgcaccgg
      241 cttgcgcggc ggggttttcc acatgcgatt gaatcacagt tgcgcctcgc gcggccggct
      301 gtgccaatcc gcctgctgga gcaggcgtga aatgagtcct agagatgaat gtttggggca
      361 cgaaggcagc gctaagcgat gaccttatcc tgggggcgca acggaccggc ggcggcgctt
      421 gcattagcgg caggcgtact catcgctccg ctatggtgcg tgtatcttcg cctgccggca
      481 gacctgggcg cgatcgccga cctccgcccc accaaagcct gtgccaagcg atgacgaaca
      541 aaatcgcctt gagtgccttc aagctcgtcg tcttcggcac ggcgctgttc tgcgttttcg
      601 ctttttggcg cacggccatc tccatgggcc tgcgcctgcc gctcggcccc aacgaaggct
      661 ggaacgccta tcatgcggcg gcggcgatgt cgggcgggcc gctctatccc ggcccccaca
      721 gcatgatgat caacaattat ccgcccttgt cgttctacct gatcggcgct ttcggccgcg
      781 ttctcgacga caacatcctg gccggcagga tcgtttccct gctctccttc ttccttatta
      841 tcctggggat tttcgccgcc gcgctgcgcc tgggctgcaa gcggctcgaa gcgtccttcg
      901 ccgcgctctt gttcgccggc ggcatgctcg ttttcaccga ttatgtcggt atgaacgatc
      961 ctcagatgct ggcgcacgcc attgcgatgg cggggttcct gcttctgctc aaggcgccgc
     1021 gcggaaccgt tcatgtcgcc ctcgcggcgc tgctgttcgt gctagccgtc ttcgtcaagc
     1081 acaatgtgat cgcgatggcg ctggcggcca cggtctggct cattgattac gaccgcaaga
     1141 acgcgctgcg tctggccggc ctcggcgtcg cgtttttgct cgccggcctg gttctcttcc
     1201 gtcttatcta cgggacgggg ctgctcgcag agctgatctc ggcgcgcatt tattctatag
     1261 ataatttaca cgccggattc gacacttggc tgcgctggag cttggtgccg ctcgtcggcc
     1321 tgacggtcct tgcctccctg cgctggcccg accggtatgt gcggctcgtt tccttctatg
     1381 cggcggccgg cacgatcctc ggcctttgtt tcctgggcgg cgccggcgtg gacgcgaatg
     1441 cgatgttcga cgccgacatc gcgctggcgc tgggtgcggc cttgctggtc caacaggccg
     1501 cgcgaggcgc gtttgcgccc gtcacggccg ccgcctatgt cctgccgctg cttctcggcg
     1561 cctggaccaa tgccggctgg cgtgatgccc atggctggct gcatccgatg cgggaagaga
     1621 ccgctgcggc gcaaaaggac atcgccttcc tggcggcgcg gccgggaccg gcgctgtgcg
     1681 agatgcagtc cttctgctac tgggcgcgca agcccgccgc ggtggacgtt ttcaacctca
     1741 gccagcagtt cgccaccggc gcgcgcagcg atgcaaagc
//
LOCUS       DASWPU010000661         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_6075, whole
            genome shotgun sequence.
ACCESSION   DASWPU010000661 DASWPU010000000
VERSION     DASWPU010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6075"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VGC27_14070"
     CDS             complement(<1..1052)
                     /locus_tag="VGC27_14070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY0283736.1"
                     /translation="MDRRYGWGLSGFFGRFVSVAGVVLASAAIAFATAFFLNARTRTV
                     QTNAPPPPQQMIKVAAPALPPGSQFWFSSTYKPGDTRLVEIVAAGDVMMGSADSGLNP
                     DIGPATDPAALIGDDLAAIFRRADIAFVNLEGPLYDGANGPAKNCTNCYAFRSPTIYA
                     GVLAGLGIDAVSLANNHSGDYGEAGRASTMAALRAHGIGFGGLDRDGARAATLLLPGG
                     RKAAFAAFAPNDGTLDLNDIPAAEKLIRAWKKTHDLVIVSFHGGAEGWDHVHVAWGTE
                     MFHGEDRGDVVRFAHAAIDAGADIVIGQGPHVPRAVEVYRGHLIAYSLGNFWTYNGVQ
                     TYAVSGMGPVLEAWVTP"
     gene            complement(1124..>1813)
                     /locus_tag="VGC27_14075"
     CDS             complement(1124..>1813)
                     /locus_tag="VGC27_14075"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0283737.1"
                     /translation="IGRSMERRLHAAGVPDIATLWALSPSRARAIWGSIGGERFWYAL
                     HGIDPPDIETHRQSIGHSHVLGPALRNRIAAYRVARRLLARAASRLRRTEHKAGKLTL
                     WLRVEGEGTRETAAHFSHTADTFSLLTALDRLWNGIVRQDEVLPVKHVGITLLDLIST
                     TGIAPDLFGWAPDIDERPERLRLSLAIDRLNQRYGVDTVAIGTAPMGLPAYMGAKIAF
                     NRIPERKDFAG"
BASE COUNT          341 a          591 c          588 g          293 t
ORIGIN      
        1 ggagtcaccc aggcttcgag caccggcccc atgcccgaaa ccgcgtaggt ctgcacgccg
       61 ttataggtcc agaaatttcc caggctgtag gcgatcagat ggccgcgata gacctcgacg
      121 gcgcgcggca catgcggccc ctgcccgatc acgatatccg cgcccgcgtc gatggcggcg
      181 tgggcgaagc gcacgacatc gccgcggtcc tcgccgtgga acatctcggt gccccaagcg
      241 acgtggacat gatcccagcc ttcggctccg ccgtggaagg agacgatcac cagatcgtgc
      301 gtcttcttcc aggcgcggat gagtttttcc gccgccggga tgtcgttcag gtcgagcgtg
      361 ccgtcgttgg gcgcgaaggc cgcaaaggca gctttgcgtc cgcccggaag cagaagcgtc
      421 gcggcgcggg cgccgtcgcg atcgagaccg ccgaaaccta tgccgtgcgc gcgcagagcc
      481 gccatcgtgg aggcgcggcc ggcttcgccg tagtcgccgc tatgattgtt ggcaaggctg
      541 accgcgtcga tgccgaggcc ggccagcacg cccgcgtaga tggtcgggct gcggaaggcg
      601 tagcaattcg tgcagttctt cgccggtccg ttcgctccgt cgtagagcgg tccttcgaga
      661 ttgacgaacg cgatatcggc gcgtctgaag atcgccgcca gatcgtcgcc gatcagcgcg
      721 gcgggatccg tcgcgggccc gatgtcggga ttgagaccgg aatcggcgga gcccatcatc
      781 acatcgcccg ccgcgacgat ctccaccaaa cgggtatcac ccggcttata ggtcgaggag
      841 aaccaaaact gactacccgg cggcaaggcg ggtgcggcga cctttatcat ctgttgcggc
      901 ggaggtggcg cgttcgtctg taccgtgcgc gtgcgcgcat tcagaaagaa cgccgtagca
      961 aaggcgatgg cggccgacgc gagcactacc cccgccacgc tcacgaacct gccgaagaaa
     1021 ccgctcagac cccagccgta tcgccgatcc atcgcccacc cctgcgggaa aggagattgt
     1081 agatggcaaa tatgtcaagt cgagagcggc atggttaata cggtcagccc gcgaaatcct
     1141 tgcgctccgg aatccggttg aacgcgattt tcgcgcccat ataggcgggc aagcccatcg
     1201 gcgccgtacc gattgcgacc gtgtcgacgc cataacgctg attgaggcgg tcgatggcga
     1261 gcgacaaacg cagccgttcc ggccgttcgt ctatatcggg agcccagccg aacaagtccg
     1321 gcgcgatgcc ggttgtggag atcaaatcca gcaaggtgat gccgacgtgt ttaacgggca
     1381 gaacctcatc ctggcgcacg atgccgttcc acaaacgatc aagtgccgtc aaaagagaga
     1441 acgtatcagc cgtatgggaa aaatgcgccg cggtttcgcg cgtgccctcg ccctccacgc
     1501 gcagccacaa cgtcagcttg cccgccttgt gttcggtgcg gcgcagccgg ctcgccgccc
     1561 gcgccagcag gcgccgcgct acccgataag cggcgatgcg gttgcgcaac gccggtccca
     1621 gcacgtggct gtggccgatc gattggcggt gcgtttcgat atccggcgga tcgatgccgt
     1681 gcagcgcgta ccagaaccgc tcgccgccga tggaacccca gatggcgcgc gcccgcgaag
     1741 gcgacagcgc ccacaaagtc gcgatatcgg ggacgccggc ggcatgaaga cggcgctcca
     1801 tgctgcggcc aat
//
LOCUS       DASWPU010000662         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U3475 k141_12048,
            whole genome shotgun sequence.
ACCESSION   DASWPU010000662 DASWPU010000000
VERSION     DASWPU010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959293
            Sequence Read Archive: SRR10968254
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:19:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,779
            Genes (coding)                    :: 2,749
            CDSs (with protein)               :: 2,749
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 3 of 30
            Pseudo Genes (incomplete)         :: 21 of 30
            Pseudo Genes (internal stop)      :: 9 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12048"
                     /isolate="SMAG_U3475"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66729653 N 14.70436811 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VGC27_14080"
     CDS             complement(<1..249)
                     /locus_tag="VGC27_14080"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0283738.1"
                     /translation="MDALTLLHERRSCTALQAPAPQGEALDNILRAALRVPDFQGLHP
                     YEFIIACGEGLDRLGAVMQDAAHEAKLPQSVVERARRMP"
     gene            complement(254..1051)
                     /locus_tag="VGC27_14085"
     CDS             complement(254..1051)
                     /locus_tag="VGC27_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0283739.1"
                     /translation="MTKIPLSLTILGGHDKSGNPERMKLVLKAGDVLCIVGPTGSGKS
                     QLLSDIECLAQGDTPSGRRVFIDGAPSAANGRGCADGKLIAQISQNMNFVVDLAVGEF
                     LAMHADCRMVTGRGEMVRDVIACANELTGEPFSAEISLTQLSGGQTRALMIADTALLS
                     ASPVVLIDEIENAGIDRKRSLELLIAKEKIVLISTHDPILALLGGRRIVIRNGAVADI
                     IETSAAEKANLSSLDAIDRGLMRLRHCLRSGERIDAPLAWLAEVEKA"
     gene            complement(1048..1746)
                     /locus_tag="VGC27_14090"
     CDS             complement(1048..1746)
                     /locus_tag="VGC27_14090"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0283740.1"
                     /translation="MRVITIAGPPSVGKTSVAIKTAKSLAASGRRIGIVKFDALSTTD
                     DELYERAGLPVVVGLSGSVCPDHFFVSNIDDCLGWGRSIGVDILIAESAGLCNRCSPH
                     IRDMLAVCVVDNLSGIHTPRKIGPMLKLADVVVVTKGDIVSQAEREVFAFNVLRANPK
                     ATIISFNGITGQGASALARHIAEAPHMDGLEGRRLRFPMPSAVCPYCIGETAIGCDHQ
                     RGNVRKMTFDGLPA"
BASE COUNT          364 a          588 c          576 g          340 t
ORIGIN      
        1 gggcatgcgc cgggcgcgtt cgacgaccga ttgcggcaat tttgcctcat gcgcggcgtc
       61 ctgcatcacc gcacccaagc gatcgagccc ctcgccgcaa gcaatgatga attcataggg
      121 atgcaggcct tggaaatccg gcacccgcaa ggcggcccgc agaatattgt cgagcgcctc
      181 gccttgcggc gccggcgctt gcaaggcggt acaggaccgc cgttcgtgca gcagggtcaa
      241 ggcgtccatt gccctaagcc ttttcgactt ccgccagcca agccagcggg gcatctatcc
      301 gctcgccgga acgcaggcag tgacgcaacc gcatcaagcc gcggtcgatc gcgtccaggc
      361 tggagaggtt ggctttttcc gcggcgctcg tctcaataat gtcggcaacg gcaccgttgc
      421 ggatcacgat ccgccgcccg cccagcaagg cgagaatagg atcgtgggtc gagatcagaa
      481 cgattttttc cttggcaatc aaaagctcca gcgaacgctt gcggtcgatc ccggcgttct
      541 cgatttcatc gatcagcacc accggcgagg cggagagaag cgcggtatcg gcaatcatca
      601 aggcgcgggt ctgtccgccg ctgagctggg tcagcgagat ttcggcggaa aacggctctc
      661 cggtcagttc gttggcgcag gcgatgacgt cgcgcaccat ctcgccgcgt cccgtcacca
      721 tgcggcaatc ggcatgcatg gcaaggaatt cgccgaccgc aagatcgacc acgaaattca
      781 tgttttgcga tatttgggcg atcagcttgc cgtcggcgca gccgcgtccg ttcgctgcgg
      841 acggcgcacc gtcgatgaaa acgcgccgcc ccgacggcgt gtcgccctgc gccaggcatt
      901 ctatgtcgga gagcaactgg ctcttgcccg agccggtcgg gccgacgatg caaagaacat
      961 cgcccgcttt gaggacgagc ttcatcctct cggggtttcc cgatttgtcg tgcccgccca
     1021 atatggtcaa tgacaatggg atctttgtca tgccggtaaa ccgtcaaaag tcatcttgcg
     1081 cacattgccg cgctgatgat cgcagccgat ggcggtttcg ccgatgcaat agggacagac
     1141 ggcggagggc atgggaaagc gcaaacggcg cccttccaat ccgtccatgt gcggggcttc
     1201 ggcgatgtgg cgggcaagcg cactggcgcc ctggccggtg atgccgttga acgaaatgat
     1261 cgtcgccttc ggattggcgc gcagcacgtt aaaggcgaag acttcgcgct ccgcctgaga
     1321 gacgatatcg cccttggtga cgaccaccac atcggccagc ttcagcatcg gtccgatctt
     1381 gcgcggggta tggatgcccg acagattatc gaccacgcaa accgccagca tgtcgcggat
     1441 atgcggcgaa cagcggttgc acagcccggc gctttccgcg atcaggatat cgaccccgat
     1501 gctgcgcccc caccccagac aatcgtcgat gttgctgacg aagaaatgat ccgggcaaac
     1561 gctgccggac agcccaacca caaccggcag accggcgcgt tcgtaaagct cgtcgtcggt
     1621 ggtcgaaagc gcatcgaact tgacgatgcc gatacgccgc cccgatgcgg caaggctttt
     1681 ggcggtcttg atggcgaccg aggttttgcc gaccgacggc ggcccggcaa tggtgatcac
     1741 gcgcatgcgc gcccccttac cggcggcgtg agatggtccg catcgcggaa aatccggcat
     1801 gccgatttca aggtgtccgc cgtggcggga tggcgaacat aatcccaacc cagccatttc
     1861 agcttggc
//
